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Sample records for acid includes usda

  1. Acid rain and related air-pollution research. A directory of USDA and state projects in CRIS. Final report

    SciTech Connect

    Not Available

    1987-05-01

    The directory is a listing of the ongoing and recently completed research projects from the Current Research Information System (CRIS) database of the U.S. Department of Agriculture (USDA), which deal with acid rain and the related effects of air pollutants on crops and livestock. The projects were identified in March 1986, and include research active as of October 1982. The CRIS database contains projects conducted or sponsored by the USDA, State agricultural experiment stations and land-grant institutions, State forestry schools and other cooperating State institutions. CRIS is operated by the Cooperative State Research Service, USDA. Project abstracts appear in the main entry section, which is organized into chapters based upon plant, animal and natural resource categories used for classifying projects in the CRIS database. Projects assigned more than one category are repeated in each of the applicable chapters. Additional points of access are provided by a keyword/title index, investigator index, and performing institution index.

  2. Screening of the entire USDA castor germplasm collection for oil content and fatty acid composition for optimum biodiesel production.

    PubMed

    Wang, Ming Li; Morris, J Bradley; Tonnis, Brandon; Pinnow, David; Davis, Jerry; Raymer, Paul; Pederson, Gary A

    2011-09-14

    Castor has tremendous potential as a feedstock for biodiesel production. The oil content and fatty acid composition in castor seed are important factors determining the price for production and affecting the key fuel properties of biodiesel. There are 1033 available castor accessions collected or donated from 48 countries worldwide in the USDA germplasm collection. The entire castor collection was screened for oil content and fatty acid composition by nuclear magnetic resonance (NMR) and gas chromatography (GC), respectively. Castor seeds on the average contain 48.2% oil with significant variability ranging from 37.2 to 60.6%. Methyl esters were prepared from castor seed by alkaline transmethylation. GC analysis of methyl esters confirmed that castor oil was composed primarily of eight fatty acids: 1.48% palmitic (C16:0), 1.58% stearic (C18:0), 4.41% oleic (C18:1), 6.42% linoleic (C18:2), 0.68% linolenic (C18:3), 0.45% gadoleic (C20:1), 84.51% ricinoleic (C18:1-1OH), and 0.47% dihydroxystearic (C18:0-2OH) acids. Significant variability in fatty acid composition was detected among castor accessions. Ricinoleic acid (RA) was positively correlated with dihydroxystearic acid (DHSA) but highly negatively correlated with the five other fatty acids except linolenic acid. The results for oil content and fatty acid composition obtained from this study will be useful for end-users to explore castor germplasm for biodiesel production.

  3. Effects of USDA beef quality grade and cooking on fatty acid composition of neutral and polar lipid fractions.

    PubMed

    Legako, J F; Dinh, T T N; Miller, M F; Brooks, J C

    2015-02-01

    The effects of USDA beef quality grade (QG; Prime, Low Choice, and Standard; n=8) and cooking (RC) on fatty acid (FA) concentrations (mg/g dry matter) and percentages of neutral and polar lipid fractions (NL and PL, respectively)from strip steaks were explored. An increase in QG led to an accumulation of most FA, especially in the NL fraction (P < 0.001). Common effects on FA percentages were two-way interactions of either QG or RC with LF (P ≤ 0.019). Fatty acids were affected differently by QG and RC depending on their originating LF. Monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) percentages of the PL were dependent on QG (P ≤ 0.014). Cooking and QG had minimal impact on FA percentages of the NL, however, greatly influenced PL MUFA and PUFA percentages (P b 0.001). There was evidence indicating that dry heat cookery affected not only PUFA, as generally thought, but also the MUFA of PL fraction.

  4. 2,4-Dichlorophenoxyacetic acid (2,4-D)- and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)-degrading gene cluster in the soybean root-nodulating bacterium Bradyrhizobium elkanii USDA94.

    PubMed

    Hayashi, Shohei; Sano, Tomoki; Suyama, Kousuke; Itoh, Kazuhito

    2016-01-01

    Herbicides 2,4-dichlorophenoxyacetic acid (2,4-D)- and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)-degrading Bradyrhizobium strains possess tfdAα and/or cadABC as degrading genes. It has been reported that root-nodulating bacteria belonging to Bradyrhizobium elkanii also have tfdAα and cadA like genes but lack the ability to degrade these herbicides and that the cadA genes in 2,4-D-degrading and non-degrading Bradyrhizobium are phylogenetically different. In this study, we identified cadRABCK in the genome of a type strain of soybean root-nodulating B. elkanii USDA94 and demonstrated that the strain could degrade the herbicides when cadABCK was forcibly expressed. cadABCK-cloned Escherichia coli also showed the degrading ability. Because co-spiked phenoxyacetic acid (PAA) could induce the degradation of 2,4-D in B. elkanii USDA94, the lack of degrading ability in this strain was supposed to be due to the low inducing potential of the herbicides for the degrading gene cluster. On the other hand, tfdAα from B. elkanii USDA94 showed little potential to degrade the herbicides, but it did for 4-chlorophenoxyacetic acid and PAA. The 2,4-D-degrading ability of the cad cluster and the inducing ability of PAA were confirmed by preparing cadA deletion mutant. This is the first study to demonstrate that the cad cluster in the typical root-nodulating bacterium indeed have the potential to degrade the herbicides, suggesting that degrading genes for anthropogenic compounds could be found in ordinary non-degrading bacteria. PMID:27296963

  5. 2,4-Dichlorophenoxyacetic acid (2,4-D)- and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)-degrading gene cluster in the soybean root-nodulating bacterium Bradyrhizobium elkanii USDA94.

    PubMed

    Hayashi, Shohei; Sano, Tomoki; Suyama, Kousuke; Itoh, Kazuhito

    2016-01-01

    Herbicides 2,4-dichlorophenoxyacetic acid (2,4-D)- and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)-degrading Bradyrhizobium strains possess tfdAα and/or cadABC as degrading genes. It has been reported that root-nodulating bacteria belonging to Bradyrhizobium elkanii also have tfdAα and cadA like genes but lack the ability to degrade these herbicides and that the cadA genes in 2,4-D-degrading and non-degrading Bradyrhizobium are phylogenetically different. In this study, we identified cadRABCK in the genome of a type strain of soybean root-nodulating B. elkanii USDA94 and demonstrated that the strain could degrade the herbicides when cadABCK was forcibly expressed. cadABCK-cloned Escherichia coli also showed the degrading ability. Because co-spiked phenoxyacetic acid (PAA) could induce the degradation of 2,4-D in B. elkanii USDA94, the lack of degrading ability in this strain was supposed to be due to the low inducing potential of the herbicides for the degrading gene cluster. On the other hand, tfdAα from B. elkanii USDA94 showed little potential to degrade the herbicides, but it did for 4-chlorophenoxyacetic acid and PAA. The 2,4-D-degrading ability of the cad cluster and the inducing ability of PAA were confirmed by preparing cadA deletion mutant. This is the first study to demonstrate that the cad cluster in the typical root-nodulating bacterium indeed have the potential to degrade the herbicides, suggesting that degrading genes for anthropogenic compounds could be found in ordinary non-degrading bacteria.

  6. Joint USDA/DOE meeting

    SciTech Connect

    Not Available

    1994-07-01

    This documents contains the details from the USDA/USDOE meeting on April 11--12, 1994. Topics discussed include: genetic research, environmental research, renewable energy sources, food supply, improved fertilizers, new pesticides, research programs combining the efforts of the two agencies to develop new products for use in the agriculture industry, environmentally safe products, getting more with less money, and various other subjects dealing with how cooperation among these agencies can improve the agriculture industry.

  7. Extraterrestrial Amino Acids in Ureilites Including Almahata Sitta

    NASA Technical Reports Server (NTRS)

    Burton, A. S.; Glavin, D. P.; Callahan, M. P.; Dworkin, J. P.

    2011-01-01

    Ureilites are a class of meteorites that lack chondrules (achondrites) but have relatively high carbon abundances, averaging approx.3 wt %. Using highly sensitive liquid chromatography coupled with UV fluorescence and time-of-flight mass spectrometry (LC-FD/ToF-MS), it was recently determined that there are amino acids in. fragment 94 of the Almahata Sitta ureilite[l]. Based on the presence of amino acids that are rare in the Earth's biosphere, as well as the near-racemic enantiomeric ratios of marry of the more common amino acids, it was concluded that most of the detected amino acids were indigenous to the meteorite. Although the composition of the Almahata Sitta ureilite appears to be unlike other recovered ureilites, the discovery of amino acids in this meteorite raises the question of whether other ureilites rnav also contain amino acids. Herein we present the results of LC-FDlTo.F-MS analyses of: a sand sample from the Almahata Sitta strewn held, Almahata Sitta fragments 425 (an ordinary H5 chondrite) and 427 (ureilite), as well as an Antarctic ureilite (Allan lulls, ALHA 77257).

  8. Antiradical activity of gallic acid included in lipid interphases.

    PubMed

    Salcedo, C L; Frías, M A; Cutro, A C; Nazareno, M A; Disalvo, E A

    2014-10-01

    Polyphenols are well known as antioxidant agents and by their effects on the hydration layers of lipid interphases. Among them, gallic acid and its derivatives are able to decrease the dipole potential and to act in water as a strong antioxidant. In this work we have studied both effects on lipid interphases in monolayers and bilayers of dimyristoylphosphatidylcholine. The results show that gallic acid (GA) increases the negative surface charges of large unilamellar vesicles (LUVs) and decreases the dipole potential of the lipid interphase. As a result, positively charged radical species such as ABTS(+) are able to penetrate the membrane forming an association with GA. These results allow discussing the antiradical activity (ARA) of GA at the membrane phase which may be taking place in water spaces between the lipids.

  9. Research careers for microbiologists in the USDA Agricultural Research Service

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The United States Department of Agriculture (USDA) and the Agricultural Research Service (ARS) employees microbiologists in a wide variety of diverse positions. This includes work involving animal health, infectious diseases and food safety. Various agencies within the USDA are responsible for monit...

  10. 7 CFR 170.3 - What products may be sold at the USDA Farmers Market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false What products may be sold at the USDA Farmers Market...) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.3 What products may be sold at the USDA Farmers Market? Products that may be sold at the market include, but...

  11. 7 CFR 170.3 - What products may be sold at the USDA Farmers Market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false What products may be sold at the USDA Farmers Market...) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.3 What products may be sold at the USDA Farmers Market? Products that may be sold at the market include, but...

  12. 7 CFR 170.3 - What products may be sold at the USDA Farmers Market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false What products may be sold at the USDA Farmers Market...) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.3 What products may be sold at the USDA Farmers Market? Products that may be sold at the market include, but...

  13. 7 CFR 170.3 - What products may be sold at the USDA Farmers Market?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false What products may be sold at the USDA Farmers Market...) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.3 What products may be sold at the USDA Farmers Market? Products that may be sold at the market include, but...

  14. 7 CFR 170.3 - What products may be sold at the USDA Farmers Market?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false What products may be sold at the USDA Farmers Market...) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.3 What products may be sold at the USDA Farmers Market? Products that may be sold at the market include, but...

  15. Evaluation of a novel food composition database that includes glutamine and other amino acids derived from gene sequencing data

    PubMed Central

    Lenders, CM; Liu, S; Wilmore, DW; Sampson, L; Dougherty, LW; Spiegelman, D; Willett, WC

    2011-01-01

    Objectives To determine the content of glutamine in major food proteins. Subjects/Methods We used a validated 131-food item food frequency questionnaire (FFQ) to identify the foods that contributed the most to protein intake among 70 356 women in the Nurses’ Health Study (NHS, 1984). The content of glutamine and other amino acids in foods was calculated based on protein fractions generated from gene sequencing methods (Swiss Institute of Bioinformatics) and compared with data from conventional (USDA) and modified biochemical (Khun) methods. Pearson correlation coefficients were used to compare the participants’ dietary intakes of amino acids by sequencing and USDA methods. Results The glutamine content varied from 0.01 to to 9.49 g/100 g of food and contributed from 1 to to 33% of total protein for all FFQ foods with protein. When comparing the sequencing and Kuhn’s methods, the proportion of glutamine in meat was 4.8 vs 4.4%. Among NHS participants, mean glutamine intake was 6.84 (s.d.=2.19) g/day and correlation coefficients for amino acid between intakes assessed by sequencing and USDA methods ranged from 0.94 to 0.99 for absolute intake, −0.08 to 0.90 after adjusting for 100 g of protein, and 0.88 to 0.99 after adjusting for 1000 kcal. The between-person coefficient of variation of energy-adjusted intake of glutamine was 16%. Conclusions These data suggest that (1) glutamine content can be estimated from gene sequencing methods and (2) there is a reasonably wide variation in energy-adjusted glutamine intake, allowing for exploration of glutamine consumption and disease. PMID:19756030

  16. Catalysis of the Carbonylation of Alcohols to Carboxylic Acids Including Acetic Acid Synthesis from Methanol.

    ERIC Educational Resources Information Center

    Forster, Denis; DeKleva, Thomas W.

    1986-01-01

    Monsanto's highly successful synthesis of acetic acid from methanol and carbon monoxide illustrates use of new starting materials to replace pretroleum-derived ethylene. Outlines the fundamental aspects of the acetic acid process and suggests ways of extending the synthesis to higher carboxylic acids. (JN)

  17. Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid

    DOEpatents

    Nasarabadi, Shanavaz

    2011-01-11

    A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.

  18. USDA perspective on environmental enrichment for animals.

    PubMed

    Kulpa-Eddy, Jodie A; Taylor, Sylvia; Adams, Kristina M

    2005-01-01

    This article provides a brief historical background of the events and circumstances that led to the 1985 Animal Welfare Act (AWA) amendments. It describes the development of the regulations promulgated by the US Department of Agriculture (USDA) in 1991 as a result of these amendments, the reasoning given for the proposals, and the revisions that were made during the process. Information is included on USDA implementation of the regulations regarding exercise for dogs and environmental enhancement for nonhuman primates. Also mentioned briefly are the requirements for socialization of marine mammals and space requirements for certain other regulated warm-blooded species. These requirements apply to animal dealers (breeders and brokers), exhibitors, commercial transporters, and research facilities. The standards for exercise and environmental enhancement were different from any others previously contained in the AWA regulations, and required more research and understanding of species-specific needs by the regulated community. Finally, this article describes some of the initiatives being undertaken by the research community and USDA-Animal and Plant Health Inspection Services (APHIS)-Animal Care to provide the necessary education and guidance indicated by the violation history data.

  19. 7 CFR 996.20 - USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE MINIMUM QUALITY AND HANDLING STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.20 USDA. USDA means...

  20. Operational Use of Remote Sensing within USDA

    NASA Technical Reports Server (NTRS)

    Bethel, Glenn R.

    2007-01-01

    A viewgraph presentation of remote sensing imagery within the USDA is shown. USDA Aerial Photography, Digital Sensors, Hurricane imagery, Remote Sensing Sources, Satellites used by Foreign Agricultural Service, Landsat Acquisitions, and Aerial Acquisitions are also shown.

  1. USDA lettuce breeding and genetics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The lettuce industry of California requires continued development of improved, adapted cultivars to meet new disease and insect problems, changes in the market, and changes in growing procedures. The USDA lettuce breeding and genetics project aims to incorporate valuable traits into crisphead, mixed...

  2. 7 CFR 996.21 - USDA laboratory.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false USDA laboratory. 996.21 Section 996.21 Agriculture... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.21 USDA laboratory. USDA laboratory means laboratories of the Science and Technology Programs, Agricultural...

  3. 7 CFR 996.21 - USDA laboratory.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false USDA laboratory. 996.21 Section 996.21 Agriculture... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.21 USDA laboratory. USDA laboratory means laboratories of the Science and Technology Programs, Agricultural...

  4. 7 CFR 996.21 - USDA laboratory.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false USDA laboratory. 996.21 Section 996.21 Agriculture... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.21 USDA laboratory. USDA laboratory means laboratories of the Science and Technology Programs, Agricultural...

  5. 7 CFR 996.21 - USDA laboratory.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false USDA laboratory. 996.21 Section 996.21 Agriculture... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.21 USDA laboratory. USDA laboratory means laboratories of the Science and Technology Programs, Agricultural...

  6. 7 CFR 996.21 - USDA laboratory.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false USDA laboratory. 996.21 Section 996.21 Agriculture... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.21 USDA laboratory. USDA laboratory means laboratories of the Science and Technology Programs, Agricultural...

  7. 7 CFR 65.265 - USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false USDA. 65.265 Section 65.265 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections..., AND GINSENG General Provisions Definitions § 65.265 USDA. USDA means the United States Department...

  8. 7 CFR 996.20 - USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false USDA. 996.20 Section 996.20 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND... DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.20 USDA. USDA means...

  9. 7 CFR 996.20 - USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false USDA. 996.20 Section 996.20 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND... DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.20 USDA. USDA means...

  10. 7 CFR 65.265 - USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false USDA. 65.265 Section 65.265 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections..., AND GINSENG General Provisions Definitions § 65.265 USDA. USDA means the United States Department...

  11. 7 CFR 60.129 - USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false USDA. 60.129 Section 60.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.129 USDA. USDA means the United States Department...

  12. 7 CFR 60.129 - USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false USDA. 60.129 Section 60.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.129 USDA. USDA means the United States Department...

  13. 7 CFR 65.265 - USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false USDA. 65.265 Section 65.265 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections..., AND GINSENG General Provisions Definitions § 65.265 USDA. USDA means the United States Department...

  14. 7 CFR 60.129 - USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false USDA. 60.129 Section 60.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.129 USDA. USDA means the United States Department...

  15. 7 CFR 65.265 - USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false USDA. 65.265 Section 65.265 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections..., AND GINSENG General Provisions Definitions § 65.265 USDA. USDA means the United States Department...

  16. 7 CFR 996.20 - USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false USDA. 996.20 Section 996.20 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and... DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.20 USDA. USDA means...

  17. 7 CFR 60.129 - USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false USDA. 60.129 Section 60.129 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.129 USDA. USDA means the United States Department...

  18. 7 CFR 205.311 - USDA Seal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false USDA Seal. 205.311 Section 205.311 Agriculture... PROVISIONS NATIONAL ORGANIC PROGRAM Labels, Labeling, and Market Information § 205.311 USDA Seal. (a) The USDA seal described in paragraphs (b) and (c) of this section may be used only for raw or...

  19. 7 CFR 205.311 - USDA Seal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false USDA Seal. 205.311 Section 205.311 Agriculture... PROVISIONS NATIONAL ORGANIC PROGRAM Labels, Labeling, and Market Information § 205.311 USDA Seal. (a) The USDA seal described in paragraphs (b) and (c) of this section may be used only for raw or...

  20. 7 CFR 205.311 - USDA Seal.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false USDA Seal. 205.311 Section 205.311 Agriculture... PROVISIONS NATIONAL ORGANIC PROGRAM Labels, Labeling, and Market Information § 205.311 USDA Seal. (a) The USDA seal described in paragraphs (b) and (c) of this section may be used only for raw or...

  1. 7 CFR 205.311 - USDA Seal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false USDA Seal. 205.311 Section 205.311 Agriculture... PROVISIONS NATIONAL ORGANIC PROGRAM Labels, Labeling, and Market Information § 205.311 USDA Seal. (a) The USDA seal described in paragraphs (b) and (c) of this section may be used only for raw or...

  2. Assessment of volatile compounds, neutral and polar lipid fatty acids of four beef muscles from USDA Choice and Select graded carcasses and their relationships with consumer palatability scores and intramuscular fat content.

    PubMed

    Hunt, M R; Legako, J F; Dinh, T T N; Garmyn, A J; O'Quinn, T G; Corbin, C H; Rathmann, R J; Brooks, J C; Miller, M F

    2016-06-01

    Fatty acids (FA) in neutral and polar lipids (NL and PL) and volatile compounds were determined in Gluteus medius (GM), Longissimus lumborum (LL), Serratus ventralis (SV), and Semimembranosus (SM) muscles from upper 2/3 USDA Choice and Select quality grades (QG). Concentrations of NL FA (mg/g) were influenced by intramuscular fat (IMF) content being greater in upper 2/3 Choice compared with Select. The SV contained greater concentrations of NL FA; meanwhile, the SM contained the lowest quantities of NL FA. Percentages (g/100g of total FA) of NL SFA and MUFA were increased in beef with greater IMF content. Concentrations and percentages of PL FA had muscle specific differences between QG. Volatile compounds were primarily affected by muscle. Increases in SFA and MUFA were related with consumer liking, regardless of lipid fraction. Overall the influence of QG on SFA and MUFA was muscle specific. Therefore, each muscle may require specific considerations when considering FA, volatile compounds, and ultimately consumer liking.

  3. Effect of omega-3 fatty acids on the modification of erythrocyte membrane fatty acid content including oleic acid in peritoneal dialysis patients.

    PubMed

    An, W S; Lee, S M; Son, Y K; Kim, S E; Kim, K H; Han, J Y; Bae, H R; Park, Y

    2012-01-01

    Erythrocyte membrane fatty acids (FA), such as oleic acid, are related to acute coronary syndrome. There is no report about the effect of omega-3 FA on oleic acid in peritoneal dialysis (PD) patients. We hypothesized that omega-3 FA can modify erythrocyte membrane FA, including oleic acid, in PD patients. In a double-blind, randomized, placebo-controlled study, 18 patients who were treated with PD for at least 6 months were randomized to treatment for 12 weeks with omega-3 FA or placebo. Erythrocyte membrane FA content was measured by gas chromatography at baseline and after 12 weeks. The erythrocyte membrane content of eicosapentaenoic acid and docosahexaenoic acid was significantly increased and saturated FA and oleic acid were significantly decreased in the omega-3 FA supplementation group after 12 weeks compared to baseline. In conclusion, erythrocyte membrane FA content, including oleic acid, was significantly modified by omega-3 FA supplementation for 12 weeks in PD patients.

  4. USDA-ARS Riesel Watersheds, Riesel, Texas, USA: Water quality research database

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The 75 year legacy database including discharge, sediment loss, land management, and meteorological data for the USDA-ARS Riesel Watersheds has been available on the web for more than a decade (www.ars.usda.gov/spa/hydro-data); however, only recently have additional water quality data been added. T...

  5. Genotyping by sequencing reveals the genetic diversity of the USDA pisum diversity collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA expanded Pisum Single Plant (PSP) core collection is a unique resource that represents the breadth of the genetic diversity of the genus in an inbred format that facilitates genetic study. The collection includes inbred accessions from the refined pea core collection, parent lines of USDA r...

  6. USDA, ARS Soft Wheat Quality Laboratory, Annual Report 2011

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The report describes new activities for the lab including the a transitions in the laboratory and activites on the USDA, NIFA-funded Triticeae CAP project. Recent research on milling and quality evaluations, data management, molecular evaluations, stem rust resistance from the lab are highlighted, ...

  7. Climate programs update: USDA Southwest Regional Climate Hub update

    Technology Transfer Automated Retrieval System (TEKTRAN)

    PROGRAM OVERVIEW: The overarching goal of the USDA SW Climate Hub is to assist farmers, ranchers and foresters in addressing the effects of climate change including prolonged drought, increased insect outbreaks and severe wildfires. In the first year of operations, the SW Climate Hub (est. Februa...

  8. USDA Mosquito Control Product Research for the US Military

    Technology Transfer Automated Retrieval System (TEKTRAN)

    New techniques that were developed at the USDA to protect deployed military troops from the threat of vector-borne diseases and are also applicable for use by civilian mosquito control program use are described. Techniques to be illustrated include: (1) novel military personal protection methods, (2...

  9. Novel USDA Mosquito Control Techniques for the US Military

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Novel techniques that we developed at the USDA to protect deployed military troops from the threat of vector-borne diseases are described. Some of the methods developed included (1) novel military personal protection methods, (2) barrier treatments of artificial materials and natural vegetation, and...

  10. What's New in USDA's Food Distribution Program for Schools? School Programs USDA Foods Update. Volume 14, Issue 1

    ERIC Educational Resources Information Center

    US Department of Agriculture, 2009

    2009-01-01

    This paper provides an update on all the new products and projects that U.S. Department of Agriculture (USDA) Food and Nutrition Service (FNS) has been working on, in its effort to help schools meet the nutritional needs of the students and to help in the President's challenge to End Childhood Hunger by 2015. This issue includes: (1) Back to…

  11. Fatty Acid Composition of Egg Yolk from Chickens Fed a Diet including Marigold (Tagetes erecta L.)

    PubMed Central

    Altuntaş, A.; Aydin, R.

    2014-01-01

    The objective of this study was to determine the effects of diet supplemented with marigold on egg yolk fatty acid composition and egg quality parameters. Sixty hens were assigned into three groups and fed diets supplemented with 0 (control), 10 g kg−1, or 20 g kg−1 marigold for 42 days. Eggs collected at the 6th week of the study were analyzed for fatty acid analysis. Laying performance, egg quality parameters, and feed intake were also evaluated. Yolk color scores in the group fed the 20 g kg−1 marigold-supplemented diet were found greater than control (10.77 versus 9.77). Inclusion of 20 g kg−1 marigold in diet influenced egg weights adversely compared to the control. Diet supplemented with 10 g kg−1 or 20 g kg−1 marigold increased the levels of C16:0 and C18:0 and decreased levels of C16:1 (n-7) and C18:1 (n-9) in the egg yolk. Also, diet including marigold increased total saturated fatty acids (SFA) and decreased monounsaturated fatty acids (MUFA) in the egg yolk. PMID:25587451

  12. Fatty Acid Composition of Egg Yolk from Chickens Fed a Diet including Marigold (Tagetes erecta L.).

    PubMed

    Altuntaş, A; Aydin, R

    2014-01-01

    The objective of this study was to determine the effects of diet supplemented with marigold on egg yolk fatty acid composition and egg quality parameters. Sixty hens were assigned into three groups and fed diets supplemented with 0 (control), 10 g kg(-1), or 20 g kg(-1) marigold for 42 days. Eggs collected at the 6th week of the study were analyzed for fatty acid analysis. Laying performance, egg quality parameters, and feed intake were also evaluated. Yolk color scores in the group fed the 20 g kg(-1) marigold-supplemented diet were found greater than control (10.77 versus 9.77). Inclusion of 20 g kg(-1) marigold in diet influenced egg weights adversely compared to the control. Diet supplemented with 10 g kg(-1) or 20 g kg(-1) marigold increased the levels of C16:0 and C18:0 and decreased levels of C16:1 (n-7) and C18:1 (n-9) in the egg yolk. Also, diet including marigold increased total saturated fatty acids (SFA) and decreased monounsaturated fatty acids (MUFA) in the egg yolk. PMID:25587451

  13. 7 CFR 1250.509 - USDA costs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false USDA costs. 1250.509 Section 1250.509 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Regulations General § 1250.509 USDA costs. Pursuant to § 1250.347 of the Order, the Board shall pay...

  14. 7 CFR 1250.509 - USDA costs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false USDA costs. 1250.509 Section 1250.509 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Regulations General § 1250.509 USDA costs. Pursuant to § 1250.347 of the Order, the Board shall pay...

  15. 7 CFR 1250.509 - USDA costs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false USDA costs. 1250.509 Section 1250.509 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Regulations General § 1250.509 USDA costs. Pursuant to § 1250.347 of the Order, the Board shall pay...

  16. 7 CFR 1250.509 - USDA costs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false USDA costs. 1250.509 Section 1250.509 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Regulations General § 1250.509 USDA costs. Pursuant to § 1250.347 of the Order, the Board shall pay...

  17. 7 CFR 1207.508 - USDA costs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false USDA costs. 1207.508 Section 1207.508 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Rules and Regulations General § 1207.508 USDA costs. Pursuant to § 1207.341 of the Plan the Board...

  18. 7 CFR 1207.508 - USDA costs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false USDA costs. 1207.508 Section 1207.508 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Rules and Regulations General § 1207.508 USDA costs. Pursuant to § 1207.341 of the Plan the Board...

  19. 7 CFR 1250.509 - USDA costs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false USDA costs. 1250.509 Section 1250.509 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Regulations General § 1250.509 USDA costs. Pursuant to § 1250.347 of the Order, the Board shall pay...

  20. 7 CFR 996.20 - USDA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE MINIMUM QUALITY AND HANDLING STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.20 USDA. USDA means...

  1. An algorithm for computing nucleic acid base-pairing probabilities including pseudoknots.

    PubMed

    Dirks, Robert M; Pierce, Niles A

    2004-07-30

    Given a nucleic acid sequence, a recent algorithm allows the calculation of the partition function over secondary structure space including a class of physically relevant pseudoknots. Here, we present a method for computing base-pairing probabilities starting from the output of this partition function algorithm. The approach relies on the calculation of recursion probabilities that are computed by backtracking through the partition function algorithm, applying a particular transformation at each step. This transformation is applicable to any partition function algorithm that follows the same basic dynamic programming paradigm. Base-pairing probabilities are useful for analyzing the equilibrium ensemble properties of natural and engineered nucleic acids, as demonstrated for a human telomerase RNA and a synthetic DNA nanostructure. PMID:15139042

  2. CycloPs: generating virtual libraries of cyclized and constrained peptides including nonnatural amino acids.

    PubMed

    Duffy, Fergal J; Verniere, Mélanie; Devocelle, Marc; Bernard, Elise; Shields, Denis C; Chubb, Anthony J

    2011-04-25

    We introduce CycloPs, software for the generation of virtual libraries of constrained peptides including natural and nonnatural commercially available amino acids. The software is written in the cross-platform Python programming language, and features include generating virtual libraries in one-dimensional SMILES and three-dimensional SDF formats, suitable for virtual screening. The stand-alone software is capable of filtering the virtual libraries using empirical measurements, including peptide synthesizability by standard peptide synthesis techniques, stability, and the druglike properties of the peptide. The software and accompanying Web interface is designed to enable the rapid generation of large, structurally diverse, synthesizable virtual libraries of constrained peptides quickly and conveniently, for use in virtual screening experiments. The stand-alone software, and the Web interface for evaluating these empirical properties of a single peptide, are available at http://bioware.ucd.ie .

  3. Amino acid composition, including key derivatives of eccrine sweat: potential biomarkers of certain atopic skin conditions.

    PubMed

    Mark, Harker; Harding, Clive R

    2013-04-01

    The free amino acid (AA) composition of eccrine sweat is different from other biological fluids, for reasons which are not properly understood. We undertook the detailed analysis of the AA composition of freshly isolated pure human eccrine sweat, including some of the key derivatives of AA metabolism, to better understand the key biological mechanisms governing its composition. Eccrine sweat was collected from the axillae of 12 healthy subjects immediately upon formation. Free AA analysis was performed using an automatic AA analyser after ninhydrin derivatization. Pyrrolidine-5-carboxylic acid (PCA) and urocanic acid (UCA) levels were determined using GC/MS. The free AA composition of sweat was dominated by the presence of serine accounting for just over one-fifth of the total free AA composition. Glycine was the next most abundant followed by PCA, alanine, citrulline and threonine, respectively. The data obtained indicate that the AA content of sweat bears a remarkable similarity to the AA composition of the epidermal protein profilaggrin. This protein is the key source of free AAs and their derivatives that form a major part of the natural moisturizing factor (NMF) within the stratum corneum (SC) and plays a major role in maintaining the barrier integrity of human skin. As perturbations in the production of NMF can lead to abnormal barrier function and can arise as a consequence of filaggrin genotype, we propose the quantification of AAs in sweat may serve as a non-invasive diagnostic biomarker for certain atopic skin conditions, that is, atopic dermatitis (AD).

  4. Fatty acid composition including cis-9, trans-11 CLA of cooked ground lamb

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Little information is available on effect of cooking on beneficial fatty acids such as conjugated linoleic acid (CLA) and n-3 polyunsaturated fatty acids (PUFA). The objective of this study was to examine impact of cooking on the FA composition of ground lamb of two different muscles. Samples were p...

  5. USDA Stakeholder Workshop on Animal Bioinformatics: Summary and Recommendations.

    PubMed

    Hamernik, Debora L; Adelson, David L

    2003-01-01

    An electronic workshop was conducted on 4 November-13 December 2002 to discuss current issues and needs in animal bioinformatics. The electronic (e-mail listserver) format was chosen to provide a relatively speedy process that is broad in scope, cost-efficient and easily accessible to all participants. Approximately 40 panelists with diverse species and discipline expertise communicated through the panel e-mail listserver. The panel included scientists from academia, industry and government, in the USA, Australia and the UK. A second 'stakeholder' e-mail listserver was used to obtain input from a broad audience with general interests in animal genomics. The objectives of the electronic workshop were: (a) to define priorities for animal genome database development; and (b) to recommend ways in which the USDA could provide leadership in the area of animal genome database development. E-mail messages from panelists and stakeholders are archived at http://genome.cvm.umn.edu/bioinfo/. Priorities defined for animal genome database development included: (a) data repository; (b) tools for genome analysis; (c) annotation; (d) practical application of genomic data; and (e) a biological framework for DNA sequence. A stable source of funding, such as the USDA Agricultural Research Service (ARS), was recommended to support maintenance of data repositories and data curation. Continued support for competitive grants programs within the USDA Cooperative State Research, Education and Extension Service (CSREES) was recommended for tool development and hypothesis-driven research projects in genome analysis. Additional stakeholder input will be required to continuously refine priorities and maximize the use of limited resources for animal bioinformatics within the USDA. PMID:18629125

  6. Influence of Fatty Acid Precursors, Including Food Preservatives, on the Growth and Fatty Acid Composition of Listeria monocytogenes at 37 and 10°C ▿

    PubMed Central

    Julotok, Mudcharee; Singh, Atul K.; Gatto, Craig; Wilkinson, Brian J.

    2010-01-01

    Listeria monocytogenes is a food-borne pathogen that grows at refrigeration temperatures and increases its content of anteiso-C15:0 fatty acid, which is believed to be a homeoviscous adaptation to ensure membrane fluidity, at these temperatures. As a possible novel approach for control of the growth of the organism, the influences of various fatty acid precursors, including branched-chain amino acids and branched- and straight-chain carboxylic acids, some of which are also well-established food preservatives, on the growth and fatty acid composition of the organism at 37°C and 10°C were studied in order to investigate whether the organism could be made to synthesize fatty acids that would result in impaired growth at low temperatures. The results indicate that the fatty acid composition of L. monocytogenes could be modulated by the feeding of branched-chain amino acid, C4, C5, and C6 branched-chain carboxylic acid, and C3 and C4 straight-chain carboxylic acid fatty acid precursors, but the growth-inhibitory effects of several preservatives were independent of effects on fatty acid composition, which were minor in the case of preservatives metabolized via acetyl coenzyme A. The ability of a precursor to modify fatty acid composition was probably a reflection of the substrate specificities of the first enzyme, FabH, in the condensation of primers of fatty acid biosynthesis with malonyl acyl carrier protein. PMID:20048057

  7. Influence of fatty acid precursors, including food preservatives, on the growth and fatty acid composition of Listeria monocytogenes at 37 and 10degreesC.

    PubMed

    Julotok, Mudcharee; Singh, Atul K; Gatto, Craig; Wilkinson, Brian J

    2010-03-01

    Listeria monocytogenes is a food-borne pathogen that grows at refrigeration temperatures and increases its content of anteiso-C(15:0) fatty acid, which is believed to be a homeoviscous adaptation to ensure membrane fluidity, at these temperatures. As a possible novel approach for control of the growth of the organism, the influences of various fatty acid precursors, including branched-chain amino acids and branched- and straight-chain carboxylic acids, some of which are also well-established food preservatives, on the growth and fatty acid composition of the organism at 37 degrees C and 10 degrees C were studied in order to investigate whether the organism could be made to synthesize fatty acids that would result in impaired growth at low temperatures. The results indicate that the fatty acid composition of L. monocytogenes could be modulated by the feeding of branched-chain amino acid, C(4), C(5), and C(6) branched-chain carboxylic acid, and C(3) and C(4) straight-chain carboxylic acid fatty acid precursors, but the growth-inhibitory effects of several preservatives were independent of effects on fatty acid composition, which were minor in the case of preservatives metabolized via acetyl coenzyme A. The ability of a precursor to modify fatty acid composition was probably a reflection of the substrate specificities of the first enzyme, FabH, in the condensation of primers of fatty acid biosynthesis with malonyl acyl carrier protein.

  8. Fatty acid-releasing activities in Sinorhizobium meliloti include unusual diacylglycerol lipase

    PubMed Central

    Sahonero-Canavesi, Diana X.; Sohlenkamp, Christian; Sandoval-Calderón, Mario; Lamsa, Anne; Pogliano, Kit; López-Lara, Isabel M.; Geiger, Otto

    2016-01-01

    Summary Phospholipids are well known for their membrane forming properties and thereby delimit any cell from the exterior world. In addition, membrane phospholipids can act as precursors for signals and other biomolecules during their turnover. Little is known about phospholipid signalling, turnover and remodelling in bacteria. Recently, we showed that a FadD-deficient mutant of Sinorhizobium meliloti, unable to convert free fatty acids to their coenzyme A derivatives, accumulates free fatty acids during the stationary phase of growth. Enzymatic activities responsible for the generation of these free fatty acids were unknown in rhizobia. Searching the genome of S. meliloti, we identified a potential lysophospholipase (SMc04041) and two predicted patatin-like phospholipases A (SMc00930, SMc01003). Although SMc00930 as well as SMc01003 contribute to the release of free fatty acids in S. meliloti, neither one can use phospholipids as substrates. Here we show that SMc01003 converts diacylglycerol to monoacylglycerol and a fatty acid, and that monoacylglycerol can be further degraded by SMc01003 to another fatty acid and glycerol. A SMc01003-deficient mutant of S. meliloti transiently accumulates diacylglycerol, suggesting that SMc01003 also acts as diacylglycerol lipase (DglA) in its native background. Expression of the DglA lipase in Escherichia coli causes lysis of cells in stationary phase of growth. PMID:25711932

  9. Fatty acid-releasing activities in Sinorhizobium meliloti include unusual diacylglycerol lipase.

    PubMed

    Sahonero-Canavesi, Diana X; Sohlenkamp, Christian; Sandoval-Calderón, Mario; Lamsa, Anne; Pogliano, Kit; López-Lara, Isabel M; Geiger, Otto

    2015-09-01

    Phospholipids are well known for their membrane-forming properties and thereby delimit any cell from the exterior world. In addition, membrane phospholipids can act as precursors for signals and other biomolecules during their turnover. Little is known about phospholipid signalling, turnover and remodelling in bacteria. Recently, we showed that a FadD-deficient mutant of Sinorhizobium meliloti, unable to convert free fatty acids to their coenzyme A derivatives, accumulates free fatty acids during the stationary phase of growth. Enzymatic activities responsible for the generation of these free fatty acids were unknown in rhizobia. Searching the genome of S. meliloti, we identified a potential lysophospholipase (SMc04041) and two predicted patatin-like phospholipases A (SMc00930, SMc01003). Although SMc00930 as well as SMc01003 contribute to the release of free fatty acids in S. meliloti, neither one can use phospholipids as substrates. Here we show that SMc01003 converts diacylglycerol to monoacylglycerol and a fatty acid, and that monoacylglycerol can be further degraded by SMc01003 to another fatty acid and glycerol. A SMc01003-deficient mutant of S. meliloti transiently accumulates diacylglycerol, suggesting that SMc01003 also acts as diacylglycerol lipase (DglA) in its native background. Expression of the DglA lipase in Escherichia coli causes lysis of cells in stationary phase of growth.

  10. Altering the fatty acids in milk fat by including canola seed in dairy cattle diets.

    PubMed

    Chichlowski, M W; Schroeder, J W; Park, C S; Keller, W L; Schimek, D E

    2005-09-01

    The objective was to evaluate the effects of feeding ground canola seed on the fatty acid profile, yield, and composition of milk from dairy cows. Twenty-four multiparous Holstein cows (548.3 +/- 11.9 kg body weight and 28 +/- 9 d in lactation) were randomly assigned to 1 of 2 treatments: Control (CON) or ground canola seed treatment (GCS) with 14% [of diet dry matter (DM)] of the total ration as ground canola seed containing 34% lipid. Diets contained 20% crude protein, but varied in net energy as a result of fat content differences of 2.5% and 6.4% (DM) for CON and GCS, respectively. Diets were composed of corn, corn silage, alfalfa (50:50 ground hay and haylage, DM basis), soybean and blood meal, and vitamins and minerals. Mechanically extruded canola meal was used in the CON diet to adjust for the protein from canola seed in the GCS diet. Cows were housed in tie-stalls and fed and milked twice daily for 10 wk. The inclusion of ground canola seed did not alter DM intake, weight gain, or body condition score of cows. Milk fat from GCS cows had greater proportions of long-chain fatty acids (> or = 18 carbons) and a lower ratio of n-6 to n-3 fatty acids. Feeding GCS reduced the proportion of short- and medium-chain fatty acids. Milk fat from cows fed GCS had a greater proportion of vaccenic acid and tended to have a higher proportion of cis-9,trans-11 conjugated linoleic acid. Actual and 3.5% fat-corrected milk yields were similar between treatments. The milk fat and protein percentages were lower for GCS cows, but total yield of these components was similar between treatments. Milk urea nitrogen was lower and serum urea nitrogen tended to be lower in cows fed canola seed. Serum glucose, insulin, and nonesterified fatty acids were not altered, but serum triglycerides were higher in GCS cows. Ammonia and total volatile fatty acids tended to be lower in ruminal fluid from GCS cows; rumen pH was unchanged. Feeding canola seed to lactating dairy cows resulted in milk

  11. Final work plan : investigation of potential contamination at the former USDA facility in Powhattan, Kansas.

    SciTech Connect

    LaFreniere, L. M.; Environmental Science Division

    2007-02-02

    This Work Plan outlines the scope of work to be conducted to investigate the subsurface contaminant conditions at the property formerly leased by the Commodity Credit Corporation (CCC) in Powhattan, Kansas (Figure 1.1). Data obtained during this event will be used to (1) evaluate potential contaminant source areas on the property; (2) determine the vertical and horizontal extent of potential contamination; and (3) provide recommendations for future action, with the ultimate goal of assigning this site No Further Action status. The planned investigation includes groundwater monitoring requested by the Kansas Department of Health and Environment (KDHE), in accordance with Section V of the Intergovernmental Agreement between the KDHE and the Farm Service Agency of the U.S. Department of Agriculture (USDA). The work is being performed on behalf of the CCC/USDA by the Environmental Science Division of Argonne National Laboratory. A nonprofit, multidisciplinary research center operated by the University of Chicago for the U.S. Department of Energy, Argonne provides technical assistance to the CCC/USDA with environmental site characterization and remediation at former CCC/USDA grain storage facilities. Argonne issued a Master Work Plan (Argonne 2002) that has been approved by the KDHE. The Master Work Plan describes the general scope of all investigations at former CCC/USDA facilities in Kansas and provides guidance for these investigations. It should be consulted for the complete details of plans for work associated with the former CCC/USDA facility at Powhattan.

  12. Case Studies in Systems Chemistry. Final Report. [Includes Complete Case Study, Carboxylic Acid Equilibria

    ERIC Educational Resources Information Center

    Fleck, George

    This publication was produced as a teaching tool for college chemistry. The book is a text for a computer-based unit on the chemistry of acid-base titrations, and is designed for use with FORTRAN or BASIC computer systems, and with a programmable electronic calculator, in a variety of educational settings. The text attempts to present computer…

  13. Antibiosis of some lactic acid bacteria including Lactobacillus acidophilus toward Listeria monocytogenes.

    PubMed

    Raccach, M; McGrath, R; Daftarian, H

    1989-08-01

    Eleven strains of lactic acid bacteria were tested by the 'spot' on the 'lawn' method for their antagonistic activity against four strains of Listeria monocytogenes. Four out of the five strains of lactic acid bacteria most antagonistic toward the pathogen were those cultures known to produce bacteriocins. Four other strains of lactic acid bacteria were not antagonistic against Listeria by this method. Seventeen inhibition zones of the pathogen were obtained at 25 degrees C as compared to 10 at 32 degrees C. Lactobacillus acidophilus strains NU-A and 88, growing in the presence of L. monocytogenes in milk prevented the latter from attaining populations it would have in pure culture (P less than 0.01). 10(1.4)-10(3.5) lower numbers were noted. L. acidophilus in most cases exhibited a bacteriostatic effect toward the pathogen except for strain 88 which appeared to have a bactericidal effect (P less than 0.01) against Listeria strain OH. The lactobacilli reduced the pH of the milk to 4.7 over a 24 h period, showing that acid played a role in the observed antibiosis.

  14. Kinetic model of water disinfection using peracetic acid including synergistic effects.

    PubMed

    Flores, Marina J; Brandi, Rodolfo J; Cassano, Alberto E; Labas, Marisol D

    2016-01-01

    The disinfection efficiencies of a commercial mixture of peracetic acid against Escherichia coli were studied in laboratory scale experiments. The joint and separate action of two disinfectant agents, hydrogen peroxide and peracetic acid, were evaluated in order to observe synergistic effects. A kinetic model for each component of the mixture and for the commercial mixture was proposed. Through simple mathematical equations, the model describes different stages of attack by disinfectants during the inactivation process. Based on the experiments and the kinetic parameters obtained, it could be established that the efficiency of hydrogen peroxide was much lower than that of peracetic acid alone. However, the contribution of hydrogen peroxide was very important in the commercial mixture. It should be noted that this improvement occurred only after peracetic acid had initiated the attack on the cell. This synergistic effect was successfully explained by the proposed scheme and was verified by experimental results. Besides providing a clearer mechanistic understanding of water disinfection, such models may improve our ability to design reactors. PMID:26819382

  15. Research update USDA-PBARC and CTAHR

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A new management method was developed for consistent flowering and fruiting of ‘Kaimana’ lychee at the USDA/ARS PBARC lychee orchard. This method consisted of pruning lychee trees after harvest followed by a foliar fertilizer application one week after pruning. Average production of ‘Kaimana’ tree...

  16. USDA-EPA Collaborative Ammonia Research

    EPA Science Inventory

    In 2014, a work group was formed between USDA and EPA to facilitate information exchange on ammonia emissions from agriculture, air quality impacts and emission mitigation options and to identify opportunities for collaboration. This document provides background on the work grou...

  17. USDA dietary supplement ingredient database, release 2

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Nutrient Data Laboratory (NDL),Beltsville Human Nutrition Research Center (BHNRC), Agricultural Research Service (ARS), USDA, in collaboration with the Office of Dietary Supplements, National Institutes of Health (ODS/NIH) and other federal agencies has developed a Dietary Supplement Ingredient ...

  18. USDA/ARS Organic Production Research

    Technology Transfer Automated Retrieval System (TEKTRAN)

    For much of its history, USDA/ARS had little to do with research on organic agriculture, however research in organic systems has made considerable gains at the agency over the past decade. In the 1980's and 1990's, as the organic food industry was taking off, ARS researchers who wanted to serve orga...

  19. USDA Southwest climate hub for climate change

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Southwest (SW) Climate Hub was created in February 2014 to develop risk adaptation and mitigation strategies for coping with climate change effects on agricultural productivity. There are seven regional hubs across the country with three subsidiary hubs. The SW Climate Hub Region is made up...

  20. The USDA Pearl Millet Germplasm Collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA National Plant Germplasm System pearl millet collection is maintained at the Plant Genetic Resources Conservation Unit located in Griffin, Ga. The germplasm collection contains 1297 unique accessions collected from 31 different countries. The majority of the accessions were collected or d...

  1. 7 CFR 1207.508 - USDA costs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false USDA costs. 1207.508 Section 1207.508 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION...

  2. 7 CFR 1207.508 - USDA costs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false USDA costs. 1207.508 Section 1207.508 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION...

  3. 7 CFR 1207.508 - USDA costs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false USDA costs. 1207.508 Section 1207.508 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION...

  4. An update on USDA swine gene banking

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA, Agricultural Research Service, National Animal Germplasm Program (NAGP) collects, preserves, evaluates and utilizes germplasm (semen, eggs, embryos, DNA) from all of the agricultural species in the US in order to create a secure collection. The material can be released for any of a number...

  5. 7 CFR 996.22 - USDA-approved laboratory.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false USDA-approved laboratory. 996.22 Section 996.22... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.22 USDA-approved laboratory. USDA-approved laboratory means laboratories approved by the Science and Technology...

  6. 7 CFR 996.22 - USDA-approved laboratory.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false USDA-approved laboratory. 996.22 Section 996.22... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.22 USDA-approved laboratory. USDA-approved laboratory means laboratories approved by the Science and Technology...

  7. 7 CFR 996.22 - USDA-approved laboratory.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false USDA-approved laboratory. 996.22 Section 996.22... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.22 USDA-approved laboratory. USDA-approved laboratory means laboratories approved by the Science and Technology...

  8. 7 CFR 996.22 - USDA-approved laboratory.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false USDA-approved laboratory. 996.22 Section 996.22... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.22 USDA-approved laboratory. USDA-approved laboratory means laboratories approved by the Science and Technology...

  9. 7 CFR 996.22 - USDA-approved laboratory.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false USDA-approved laboratory. 996.22 Section 996.22... STANDARDS FOR DOMESTIC AND IMPORTED PEANUTS MARKETED IN THE UNITED STATES Definitions § 996.22 USDA-approved laboratory. USDA-approved laboratory means laboratories approved by the Science and Technology...

  10. 7 CFR 930.5 - Department or USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Department or USDA. 930.5 Section 930.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Definitions § 930.5 Department or USDA. Department or USDA means the United States Department of Agriculture....

  11. 7 CFR 983.10 - Department or USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Department or USDA. 983.10 Section 983.10 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements..., ARIZONA, AND NEW MEXICO Definitions § 983.10 Department or USDA. Department or USDA means the...

  12. 7 CFR 63.3 - Department or USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Department or USDA. 63.3 Section 63.3 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... IMPROVEMENT CENTER General Provisions Definitions § 63.3 Department or USDA. Department or USDA means...

  13. 7 CFR 983.10 - Department or USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Department or USDA. 983.10 Section 983.10 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS..., ARIZONA, AND NEW MEXICO Definitions § 983.10 Department or USDA. Department or USDA means the...

  14. 7 CFR 3022.10 - Reporting to USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 15 2012-01-01 2012-01-01 false Reporting to USDA. 3022.10 Section 3022.10..., DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.10 Reporting to USDA. Following completion of an investigation into allegations of...

  15. 7 CFR 3022.10 - Reporting to USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false Reporting to USDA. 3022.10 Section 3022.10..., DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.10 Reporting to USDA. Following completion of an investigation into allegations of...

  16. 7 CFR 1217.7 - Department or USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false Department or USDA. 1217.7 Section 1217.7 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS..., and Industry Information Order Definitions § 1217.7 Department or USDA. Department or USDA means the...

  17. 7 CFR 63.3 - Department or USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Department or USDA. 63.3 Section 63.3 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... IMPROVEMENT CENTER General Provisions Definitions § 63.3 Department or USDA. Department or USDA means...

  18. 7 CFR 930.5 - Department or USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Department or USDA. 930.5 Section 930.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Definitions § 930.5 Department or USDA. Department or USDA means the United States Department of Agriculture....

  19. 7 CFR 3406.6 - USDA agency cooperator requirement.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false USDA agency cooperator requirement. 3406.6 Section... PROGRAM Program Description § 3406.6 USDA agency cooperator requirement. (a) Each application must provide documentation that at least one USDA agency or office has agreed to cooperate with the applicant institution...

  20. 7 CFR 3022.10 - Reporting to USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false Reporting to USDA. 3022.10 Section 3022.10..., DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.10 Reporting to USDA. Following completion of an investigation into allegations of...

  1. 7 CFR 930.5 - Department or USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Department or USDA. 930.5 Section 930.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Definitions § 930.5 Department or USDA. Department or USDA means the United States Department of Agriculture....

  2. 7 CFR 983.10 - Department or USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Department or USDA. 983.10 Section 983.10 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements..., ARIZONA, AND NEW MEXICO Definitions § 983.10 Department or USDA. Department or USDA means the...

  3. 7 CFR 63.3 - Department or USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Department or USDA. 63.3 Section 63.3 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... IMPROVEMENT CENTER General Provisions Definitions § 63.3 Department or USDA. Department or USDA means...

  4. 7 CFR 930.5 - Department or USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Department or USDA. 930.5 Section 930.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Definitions § 930.5 Department or USDA. Department or USDA means the United States Department of Agriculture....

  5. 48 CFR 432.770 - USDA specific funding limitations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false USDA specific funding... CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 432.770 USDA specific funding limitations. (a) The USDA is authorized to subscribe for newspapers as may be necessary to carry out its...

  6. 48 CFR 432.770 - USDA specific funding limitations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false USDA specific funding... CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 432.770 USDA specific funding limitations. (a) The USDA is authorized to subscribe for newspapers as may be necessary to carry out its...

  7. 7 CFR 1217.7 - Department or USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false Department or USDA. 1217.7 Section 1217.7 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS..., and Industry Information Order Definitions § 1217.7 Department or USDA. Department or USDA means the...

  8. 7 CFR 3022.10 - Reporting to USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Reporting to USDA. 3022.10 Section 3022.10..., DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.10 Reporting to USDA. Following completion of an investigation into allegations of...

  9. 48 CFR 432.770 - USDA specific funding limitations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false USDA specific funding... CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 432.770 USDA specific funding limitations. (a) The USDA is authorized to subscribe for newspapers as may be necessary to carry out its...

  10. 7 CFR 930.5 - Department or USDA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Department or USDA. 930.5 Section 930.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Definitions § 930.5 Department or USDA. Department or USDA means the United States Department of Agriculture....

  11. 7 CFR 1217.7 - Department or USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false Department or USDA. 1217.7 Section 1217.7 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS..., and Industry Information Order Definitions § 1217.7 Department or USDA. Department or USDA means the...

  12. 7 CFR 983.10 - Department or USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Department or USDA. 983.10 Section 983.10 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS..., ARIZONA, AND NEW MEXICO Definitions § 983.10 Department or USDA. Department or USDA means the...

  13. 7 CFR 63.3 - Department or USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Department or USDA. 63.3 Section 63.3 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... IMPROVEMENT CENTER General Provisions Definitions § 63.3 Department or USDA. Department or USDA means...

  14. New USDA rootstocks for better disease tolerance and fruit productivity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-ARS has a long-term citrus rootstock breeding program in Florida, and in the past two decades the Florida citrus industry has also provided strong financial support for USDA rootstock development. Four new rootstocks have been released by the USDA in Florida over the past 12 years. These ro...

  15. Fate of N-nitrosodimethylamine, trihalomethane and haloacetic acid precursors in tertiary treatment including biofiltration.

    PubMed

    Farré, Maria José; Reungoat, Julien; Argaud, Francois Xavier; Rattier, Maxime; Keller, Jürg; Gernjak, Wolfgang

    2011-11-01

    The presence of disinfection by-products (DBPs) such as trihalomethanes (THMs), haloacetic acids (HAAs) and N-nitrosamines in water is of great concern due to their adverse effects on human health. In this work, the removal of N-nitrosodimethylamine (NDMA), total THM and five HAA precursors from secondary effluent by biological activated carbon (BAC) is investigated at full and pilot scale. In the pilot plant two filter media, sand and granular activated carbon, are tested. In addition, we evaluate the influence of ozonation prior to BAC filtration on its performance. Among the bulk of NDMA precursors, the fate of four pharmaceuticals containing a dimethylamino moiety in the chemical structure are individually investigated. Both NDMA formation potential and each of the studied pharmaceuticals are dramatically reduced by the BAC even in the absence of main ozonation prior to the filtration. The low removal of NDMA precursors at the sand filtration in comparison to the removal of NDMA precursors at the BAC suggests that adsorption may play an important role on the removal of NDMA precursors by BAC. Contrary, the precursors for THM and HAA formation are reduced in both sand filtration and BAC indicating that the precursors for the formation of these DBPs are to some extent biodegradable.

  16. Differential regulation of host genes including hepatic fatty acid synthase in HBV-transgenic mice.

    PubMed

    Zhang, Hongmin; Li, Hong; Yang, Yixuan; Li, Sanglin; Ren, Hong; Zhang, Dazhi; Hu, Huaidong

    2013-06-01

    Hepatitis B virus (HBV) is the most common of the hepatitis viruses that cause chronic liver infections in humans, and it is considered to be a major global health problem. To gain a better understanding of HBV pathogenesis, and identify novel putative targets for anti-HBV therapy, this study was designed to elucidate the differential expression of host proteins in liver tissue from HBV-transgenic mice. Liver samples from two groups, (1) HBV-transgenic (Tg) mice, (2) corresponding background normal mice, wild-type (WT) mice, were collected and subjected to iTRAQ and mass spectrometry analysis. In total, 1950 unique proteins were identified, and 68 proteins were found to be differentially expressed in HBV-Tg mice as compared with that in WT mice. Several differentially expressed proteins were further validated by real-time quantitative RT-PCR, Western blot and immunohistochemical analysis. Furthermore, the association of HBV replication with fatty acid synthase (FASN), one of the highly expressed proteins in HBV-Tg mice, was verified. Silencing of FASN expression in HepG2.2.15 cells suppressed viral replication through the IFN signaling pathway, and some downstream antiviral effectors. The implicated role of FASN in HBV replication provides an opportunity to test existing compounds against FASN for adjuvant therapy and/or treatment of HBV replication. PMID:23675653

  17. Chemical Genetics Uncovers Novel Inhibitors of Lignification, Including p-Iodobenzoic Acid Targeting CINNAMATE-4-HYDROXYLASE.

    PubMed

    Van de Wouwer, Dorien; Vanholme, Ruben; Decou, Raphaël; Goeminne, Geert; Audenaert, Dominique; Nguyen, Long; Höfer, René; Pesquet, Edouard; Vanholme, Bartel; Boerjan, Wout

    2016-09-01

    Plant secondary-thickened cell walls are characterized by the presence of lignin, a recalcitrant and hydrophobic polymer that provides mechanical strength and ensures long-distance water transport. Exactly the recalcitrance and hydrophobicity of lignin put a burden on the industrial processing efficiency of lignocellulosic biomass. Both forward and reverse genetic strategies have been used intensively to unravel the molecular mechanism of lignin deposition. As an alternative strategy, we introduce here a forward chemical genetic approach to find candidate inhibitors of lignification. A high-throughput assay to assess lignification in Arabidopsis (Arabidopsis thaliana) seedlings was developed and used to screen a 10-k library of structurally diverse, synthetic molecules. Of the 73 compounds that reduced lignin deposition, 39 that had a major impact were retained and classified into five clusters based on the shift they induced in the phenolic profile of Arabidopsis seedlings. One representative compound of each cluster was selected for further lignin-specific assays, leading to the identification of an aromatic compound that is processed in the plant into two fragments, both having inhibitory activity against lignification. One fragment, p-iodobenzoic acid, was further characterized as a new inhibitor of CINNAMATE 4-HYDROXYLASE, a key enzyme of the phenylpropanoid pathway synthesizing the building blocks of the lignin polymer. As such, we provide proof of concept of this chemical biology approach to screen for inhibitors of lignification and present a broad array of putative inhibitors of lignin deposition for further characterization. PMID:27485881

  18. Longitudinal distributions of dicarboxylic acids, ω-oxoacids, pyruvic acid, α-dicarbonyls, and fatty acids in the marine aerosols from the central Pacific including equatorial upwelling

    NASA Astrophysics Data System (ADS)

    Hoque, Mir Md. Mozammal; Kawamura, Kimitaka

    2016-03-01

    Remote marine aerosol samples (total suspended particles) were collected during a cruise in the central Pacific from Japan to Mexico (1°59'N-35°N and 171°54'E-90°58'W). The aerosol samples were analyzed for dicarboxylic acids (C2-C11), ω-oxoacids, pyruvic acid, α-dicarbonyls, and fatty acids as well as organic and elemental carbon, water-soluble organic carbon, and total nitrogen (WSTN). During the study, diacids were the most abundant compound class followed by fatty acids, ω-oxoacids, and α-dicarbonyls. Molecular compositions of diacids showed a predominance of oxalic (C2) acid followed by malonic (C3) and succinic (C4) acids. Oxalic acid comprises 74% of total diacids. This result suggests that photochemical production of oxalic acid is significant over the central Pacific. Spatial distributions of diacids, ω-oxoacids, pyruvic acid, α-dicarbonyls, and fatty acids together with total carbon and WSTN showed higher abundances in the eastern equatorial Pacific where the upwelling of high-nutrient waters followed by high biological productivity is common, indicating that their in situ production is important in the warmer central Pacific through photochemical oxidation from their gaseous and particulate precursors. This study demonstrates that there is a strong linkage in biogeochemical cycles of carbon in the sea-air interface via ocean upwelling, phytoplankton productivity, sea-to-air emissions of organic matter, and formation of secondary organic aerosols in the eastern equatorial Pacific.

  19. Relationship between USDA and Japanese beef grades.

    PubMed

    Harris, J J; Lunt, D K; Savell, J W; Hawkins, E W; Orme, L E

    1995-01-01

    Angus steers (n = 78) were feedlot-finished to approximately 703 kg live weight. The finished steers were slaughtered at a commercial packing plant, chilled, and evaluated for USDA and Japanese yield and quality-grade characteristics. The resulting carcasses were excessively heavy and fat for the domestic (US) beef industry. Over 70% of the carcasses were US Yield Grade 5, and almost 40% were Japanese Yield Score 'C'. Although excessively fat externally, fewer than 50% of the carcasses qualified for US Prime, and none of the carcasses qualified for Japanese Meat Quality Score 5. Moderate to high correlations were observed between US twelfth-rib carcass traits and Japanese sixth-rib traits; however, multiple-regression equations based on USDA carcass characteristics explained only 46 and 25% of the observed variation in Japanese marbling score and estimated percentage wholesale-cut yield, respectively.

  20. The Animal Welfare Act, USDA, & research.

    PubMed

    Koch, V Wensley

    2003-03-01

    In the above discussion, the concept and evolution of IACUC oversight of research facility animal care and use programs and common USDA citations concerning these programs was reviewed. The majority of USDA citations are program-related and involve both IACUC and veterinary care functions. Common IACUC-related citations concern inadequacies involving required information in protocols (such as rationales for the species and numbers used and descriptions of the procedures proposed), searches for alternatives to painful or distressful procedures, and minimization of pain and distress. Common veterinary care citations concern inadequacies involving veterinary care facilities, daily observation of the animals, and veterinary care itself (e.g., maintaining inadequate records or using expired medications). IACUC's are advised to ensure that their program records are comprehensive enough to demonstrate that their facility's animal care and use program complies with the AWA and USDA regulations. The overall ongoing success of self-regulation in the research industry is acknowledged, and APHIS's current concentration on the recognition and alleviation of distress, as well as pain, is noted. In the future, APHIS will continue in its oversight role as IACUC programs continue to evolve in their awareness and application of the advances in pain and distress recognition and management. Together, we will continue to work for the benefit of the animals used in research, whose welfare is so important to the quality of that very research.

  1. Genetic structural analysis for germplasm accessions in the USDA Rice World Collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rice is grouped into five genetic structures including indica, aus, aromatic, temperate japonica, and tropical japonica. A core collection having 1,785 accessions from 114 countries has been developed that is representative of the USDA rice world collection which includes over 18,000 accessions. The...

  2. Evaluation of the USDA Rice Core Collection for sheath blight disease using micro-chamber

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA rice core collection, including 1,794 accessions from 114 countries, was developed using a stratified random sampling method to represent the entire NSGC collection including over 18,000 accessions. Sheath blight (Rhizoctonia solani) is one of the most important and widely distributed disea...

  3. The USDA-ARS Walnut Gulch Experimental Watershed, Arizona, USA

    NASA Astrophysics Data System (ADS)

    Nichols, Mary; Nearing, Mark; Goodrich, Dave; Heilman, Phil

    2015-04-01

    The United States Department of Agriculture (USDA) has been supporting research and data collection at instrumented watershed throughout the country since the dust bowl era of the 1930's. In 1953, the USDA established the Walnut Gulch Experimental Watershed (WGEW) in southeastern Arizona near Tombstone to conduct hydrologic and erosion research to quantify the unique rainfall-runoff characteristics of semiarid regions and to understand the downstream effects of conservation practices implemented in watershed uplands. Instrumentation and research on the WGEW has expanded to include meteorological and flux measurements, soil moisture, and ecosystem responses. In addition, the WGEW serves as a validation site for aircraft and satellite based remotely sensed instruments. Core measurements have been used to quantify semiarid rainfall, runoff, infiltration, and transmission losses; develop and validate simulation models, and support broader, regional, basin scale research. The long-term database is a critical resource for advancing the scientific understanding of semiarid ecohydrological processes. The WGEW, its history, significant contributions to instrumentation development, and the current WGEW data collection program in the context of contemporary research questions will be presented.

  4. Selected nutrient contents, fatty acid composition, including conjugated linoleic acid, and retention values in separable lean from lamb rib loins as affected by external fat and cooking method.

    PubMed

    Badiani, Anna; Montellato, Lara; Bochicchio, Davide; Anfossi, Paola; Zanardi, Emanuela; Maranesi, Magda

    2004-08-11

    Proximate composition and fatty acid profile, conjugated linoleic acid (CLA) isomers included, were determined in separable lean of raw and cooked lamb rib loins. The cooking methods compared, which were also investigated for cooking yields and true nutrient retention values, were dry heating of fat-on cuts and moist heating of fat-off cuts; the latter method was tested as a sort of dietetic approach against the more traditional former type. With significantly (P < 0.05) lower cooking losses, dry heating of fat-on rib-loins produced slightly (although only rarely significantly) higher retention values for all of the nutrients considered, including CLA isomers. On the basis of the retention values obtained, both techniques led to a minimum migration of lipids into the separable lean, which was higher (P < 0.05) in dry heating than in moist heating, and was characterized by the prevalence of saturated and monounsaturated fatty acids. On the whole, the response to cooking of the class of CLA isomers (including that of the nutritionally most important isomer cis-9,trans-11) was more similar to that of the monounsaturated than the polyunsaturated fatty acids.

  5. Final work plan : investigation of potential contamination at the former USDA facility in Ramona, Kansas.

    SciTech Connect

    LaFreniere, L. M.

    2006-01-27

    This Work Plan outlines the scope of work that will be conducted to investigate the subsurface contaminant conditions at the property formerly leased by the Commodity Credit Corporation (CCC) in Ramona, Kansas (Figure 1.1). Data obtained during this event will be used to (1) evaluate potential source areas on the property, (2) determine the vertical and horizontal extent of potential contamination, and (3) provide recommendations for future actions, with the ultimate goal of assigning this site No Further Action status. The planned investigation includes groundwater monitoring requested by the Kansas Department of Health and Environment (KDHE), in accordance with Section V of the Intergovernmental Agreement between the KDHE and the Farm Service Agency of the United States Department of Agriculture (USDA). The work is being performed on behalf of the CCC/USDA by the Environmental Research Division of Argonne National Laboratory. Argonne is a nonprofit, multidisciplinary research center operated by the University of Chicago for the U.S. Department of Energy. Under the Intergovernmental Agreement, Argonne provides technical assistance to the CCC/USDA with environmental site characterization and remediation at former CCC/USDA grain storage facilities. Argonne has issued a Master Work Plan (Argonne 2002) that describes the general scope of all investigations at former CCC/USDA facilities in Kansas and provides guidance for these investigations. The Master Work Plan was approved by the KDHE. It contains materials common to investigations at locations in Kansas and should be consulted for the complete details of plans for work associated with the former CCC/USDA facility at Ramona.

  6. Food Safety Research at USDA: Hormones in Water and POPs in Food

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Large concentrated animal farms have been the subject of intense public scrutiny, in part due to concern about environmental release of endocrine disruptors, including natural hormones. Surface waters in proximity to farms were evaluated for hormones and estrogenic activity (E-Screen) by the USDA. ...

  7. Applications of UAV imagery for agricultural and environmental research at the USDA Southeast Watershed Research Lab

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The ARS is the USDA's in-house scientific research agency, whose mission is to conduct research to "develop and transfer solutions to agricultural problems of high national priority..." This includes enhancing the natural resource base and the environment, a dimension of particular relevance to the ...

  8. Program review of the USDA Center for Medical, Agricultural and Veterinary Entomology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-ARS Center for Medical, Agricultural and Veterinary Entomology (CMAVE) has a history that starts in 1932 in Orlando to develop methods to control mosquitoes, including malaria vectors under conditions simulating those of the south Pacific jungles, and other insects affecting man and animals...

  9. Genomic characterization of a core set of the USDA-NPGS Ethiopian sorghum germplasm collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Agriculture Research Service National Plant Germplasm System (NPGS) preserves the largest sorghum germplasm collection in the world, which includes 7,217 accessions from the center of diversity in Ethiopia. The characterization of this exotic germplasm at a genome-wide scale will improve co...

  10. Assessing the effects of USDA conservation programs on ecosystem services provided by wetlands

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Conservation Effects Assessment Project (CEAP) is led by the U.S. Department of Agriculture (USDA) in an effort to quantify the environmental effects of conservation programs and practices on privately owned agricultural landscapes across the United States. CEAP’s approach includes application ...

  11. Rhizoctonia root rot resistance of Beta PIs from the USDA-ARS NPGS, 2007.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Thirty-two plant introductions (PI) from the USDA-ARS National Plant Germplasm System (NPGS) (including garden beet, sugar beet, leaf beet, fodder beet, and wild beet) were evaluated for resistance to Rhizoctonia root rot. The trial was a randomized complete-block design with five replications in ...

  12. EXAMINING PHYTOSTEROLS IN NUTS AND SEEDS FOR THE USDA NATIONAL NUTRIENT DATABASE FOR STANDARD REFERENCE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Objective: The current release of the USDA National Nutrient Database for Standard Reference (SR) includes total phytosterols for 30 of the 128 nut and seed items. Twenty-two of those have values for individual sterols – Beta-sitosterol, stigmasterol, and campesterol. The current literature was st...

  13. USDA-ARS-SPA Wheat, Peanut and Other Field Crops Research Unit Annual Report

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A Report on the research activities of the Small Grains and other Crops Research Unit of the USDA-ARS, Plant Science Research Laboratory in Stillwater, Oklahoma, was compiled for WERA-066 Meeting that was held in Stillwater, Oklahoma, February 24-26, 2009. Research summaries included predicting the...

  14. USDA-ARS-SPA Wheat, Peanut and Other Field Crops Research Unit annual report

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A Report on the research activities of the Small Grains and other Crops Research Unit of the USDA-ARS, Plant Science Research Laboratory in Stillwater, Oklahoma, was compiled for WERA-066 Meeting that was held in Ft. Collins, Colorado, September 2010. Research summaries included predicting the impa...

  15. A Model of Agricultural and Food Research Excellence-USDA/ARS/WRRC

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Agricultural Research Service is the principal in-house research arm of the U.S. Department of Agriculture (USDA), responsible for solving agricultural problems of national importance. Included among ARS's 100 locations in the U.S. are regional centers such as the Western Regional Research Cent...

  16. The USDA quality grades may mislead consumers.

    PubMed

    DeVuyst, E A; Lusk, J L; DeVuyst, M A

    2014-07-01

    This study was designed to explore consumers' perceptions about and knowledge of USDA beef quality grades. Data were collected from over 1,000 consumers in online surveys in November and December 2013, and estimates were weighted to force the sample to mirror the U.S. population in terms of age, gender, education, and region of residence. When asked to rank Prime, Choice, and Select grades in terms of leanness, only 14.4% provided the correct ranking with 57.1% of respondents incorrectly indicating steaks grading Prime were the leanest. Despite perceptions that the Prime name indicated the leanest product, in a subsequent question, 55.6% of respondents thought Prime grade to be the juiciest of the 3 grades. In addition to inquiring about perceptions of the grade names, respondents also indicated perceptions of pictures of steaks. Only 14.5% of respondents correctly matched the steak pictures with their corresponding USDA quality grade name, an outcome that is statistically worse than would have occurred through pure random matching (P = 0.03). When asked to match pictures of steaks with expected prices, 54.8% of respondents incorrectly matched the picture of the Prime steak with the lowest price level. More highly educated consumers with greater preferences for steak consumption were more likely to provide correct answers. Results reveal substantial confusion over quality grading nomenclature and suggest the need for more education or for a transition toward more descriptive terminology at the retail level.

  17. Temporal changes (1997-2012) of perfluoroalkyl acids and selected precursors (including isomers) in Swedish human serum.

    PubMed

    Gebbink, Wouter A; Glynn, Anders; Berger, Urs

    2015-04-01

    Concentrations (including isomer patterns) and temporal changes (1997-2012) of perfluoroalkyl acids (PFAAs) and selected perfluorooctane sulfonate (PFOS) and perfluoroalkyl carboxylic acid (PFCA) precursors were determined in serum samples from Swedish women. Perfluorooctane sulfonamide (FOSA) and perfluorooctane sulfonamidoacetic acid (FOSAA), as well as its N-methyl and N-ethyl derivatives (MeFOSAA and EtFOSAA) were consistently detected. Highest PFOS precursor concentrations were found for EtFOSAA (before year 2000) or MeFOSAA and FOSAA (after 2000). Disappearance half-lives for all PFOS precursors were shorter compared to PFOS. 4:2/6:2 and 6:2/6:2 polyfluoroalkyl phosphate diesters (diPAPs) were detected in <60% of the samples, whereas 6:2/8:2 and 8:2/8:2 diPAPs were detected in >60% of the samples, but showed no significant change in concentrations over time. Linear and sum-branched isomers were quantified separately for three PFAAs and three precursors. Significant changes between 1997 and 2012 in the % linear isomer were observed for PFOA and FOSA (increase) and PFOS (decrease).

  18. USDA food and nutrient databases provide the infrastructure for food and nutrition research, policy, and practice.

    PubMed

    Ahuja, Jaspreet K C; Moshfegh, Alanna J; Holden, Joanne M; Harris, Ellen

    2013-02-01

    The USDA food and nutrient databases provide the basic infrastructure for food and nutrition research, nutrition monitoring, policy, and dietary practice. They have had a long history that goes back to 1892 and are unique, as they are the only databases available in the public domain that perform these functions. There are 4 major food and nutrient databases released by the Beltsville Human Nutrition Research Center (BHNRC), part of the USDA's Agricultural Research Service. These include the USDA National Nutrient Database for Standard Reference, the Dietary Supplement Ingredient Database, the Food and Nutrient Database for Dietary Studies, and the USDA Food Patterns Equivalents Database. The users of the databases are diverse and include federal agencies, the food industry, health professionals, restaurants, software application developers, academia and research organizations, international organizations, and foreign governments, among others. Many of these users have partnered with BHNRC to leverage funds and/or scientific expertise to work toward common goals. The use of the databases has increased tremendously in the past few years, especially the breadth of uses. These new uses of the data are bound to increase with the increased availability of technology and public health emphasis on diet-related measures such as sodium and energy reduction. Hence, continued improvement of the databases is important, so that they can better address these challenges and provide reliable and accurate data.

  19. USDA Nutrient Data Set for Retail Veal Cuts

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The U.S. Department of Agriculture (USDA) Nutrient Data Laboratory (NDL), in collaboration with Colorado State University, conducted a research study designed to update and expand the data on veal cuts in the USDA National Nutrient Database for Standard Reference (SR). This research has been necess...

  20. The USDA Table of Cooking Yields for Meat and Poultry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Nutrient Data Laboratory (NDL) at the USDA conducts food composition research to develop accurate, unbiased, and representative food and nutrient composition data which are released as the USDA National Nutrient Database for Standard Reference (SR). SR is used as the foundation of most other foo...

  1. 7 CFR 634.30 - Appeals in USDA administered projects.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 6 2013-01-01 2013-01-01 false Appeals in USDA administered projects. 634.30 Section 634.30 Agriculture Regulations of the Department of Agriculture (Continued) NATURAL RESOURCES... RCWP Contracts § 634.30 Appeals in USDA administered projects. The participant in a...

  2. 7 CFR 634.30 - Appeals in USDA administered projects.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 6 2012-01-01 2012-01-01 false Appeals in USDA administered projects. 634.30 Section 634.30 Agriculture Regulations of the Department of Agriculture (Continued) NATURAL RESOURCES... RCWP Contracts § 634.30 Appeals in USDA administered projects. The participant in a...

  3. 7 CFR 634.30 - Appeals in USDA administered projects.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 6 2014-01-01 2014-01-01 false Appeals in USDA administered projects. 634.30 Section 634.30 Agriculture Regulations of the Department of Agriculture (Continued) NATURAL RESOURCES... RCWP Contracts § 634.30 Appeals in USDA administered projects. The participant in a...

  4. 7 CFR 4280.107 - USDA Departmental Regulations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Program General General § 4280.107 USDA Departmental Regulations. All projects funded under this subpart are subject to the provisions of the Departmental regulations (7 CFR subtitle A), as applicable. ... 7 Agriculture 15 2012-01-01 2012-01-01 false USDA Departmental Regulations. 4280.107 Section...

  5. 7 CFR 3406.6 - USDA agency cooperator requirement.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false USDA agency cooperator requirement. 3406.6 Section... AND AGRICULTURE 1890 INSTITUTION CAPACITY BUILDING GRANTS PROGRAM Program Description § 3406.6 USDA agency cooperator requirement. (a) Each application must provide documentation that at least one...

  6. 7 CFR 634.30 - Appeals in USDA administered projects.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Appeals in USDA administered projects. 634.30 Section 634.30 Agriculture Regulations of the Department of Agriculture (Continued) NATURAL RESOURCES... RCWP Contracts § 634.30 Appeals in USDA administered projects. The participant in a...

  7. 7 CFR 4280.107 - USDA Departmental Regulations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Program General General § 4280.107 USDA Departmental Regulations. All projects funded under this subpart are subject to the provisions of the Departmental regulations (7 CFR subtitle A), as applicable. ... 7 Agriculture 15 2013-01-01 2013-01-01 false USDA Departmental Regulations. 4280.107 Section...

  8. 7 CFR 4280.107 - USDA Departmental Regulations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Program General General § 4280.107 USDA Departmental Regulations. All projects funded under this subpart are subject to the provisions of the Departmental regulations (7 CFR subtitle A), as applicable. ... 7 Agriculture 15 2014-01-01 2014-01-01 false USDA Departmental Regulations. 4280.107 Section...

  9. 7 CFR 3406.6 - USDA agency cooperator requirement.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 15 2012-01-01 2012-01-01 false USDA agency cooperator requirement. 3406.6 Section... AND AGRICULTURE 1890 INSTITUTION CAPACITY BUILDING GRANTS PROGRAM Program Description § 3406.6 USDA agency cooperator requirement. (a) Each application must provide documentation that at least one...

  10. 7 CFR 634.30 - Appeals in USDA administered projects.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Appeals in USDA administered projects. 634.30 Section 634.30 Agriculture Regulations of the Department of Agriculture (Continued) NATURAL RESOURCES... RCWP Contracts § 634.30 Appeals in USDA administered projects. The participant in a...

  11. 7 CFR 3406.6 - USDA agency cooperator requirement.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false USDA agency cooperator requirement. 3406.6 Section... AND AGRICULTURE 1890 INSTITUTION CAPACITY BUILDING GRANTS PROGRAM Program Description § 3406.6 USDA agency cooperator requirement. (a) Each application must provide documentation that at least one...

  12. 7 CFR 3406.6 - USDA agency cooperator requirement.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false USDA agency cooperator requirement. 3406.6 Section... AND AGRICULTURE 1890 INSTITUTION CAPACITY BUILDING GRANTS PROGRAM Program Description § 3406.6 USDA agency cooperator requirement. (a) Each application must provide documentation that at least one...

  13. USDA National Nutrient Database for Standard Reference, Release 20

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Nutrient Database for Standard Reference, Release 20 contains data for 7,517 food items for up to 140 food components when a complete profile is available for a food item. It replaces the previous release, SR19, issued in August 2006. Data in SR20 supersede values in printed USDA handbooks ...

  14. What's the latest on USDA citrus scion breeding?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In its 120 year history, the USDA citrus scion breeding program has provided the mainstays of the Florida specialty citrus industry. In the last few years, the USDA has released US Early Pride mandarin, US Seedless Pineapple sweet orange and most recently, US Furr/US Furr-ST mandarin. Clearly huangl...

  15. 48 CFR 432.770 - USDA specific funding limitations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false USDA specific funding limitations. 432.770 Section 432.770 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 432.770 USDA specific funding limitations....

  16. 48 CFR 432.770 - USDA specific funding limitations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false USDA specific funding limitations. 432.770 Section 432.770 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 432.770 USDA specific funding limitations....

  17. Campylobacter Database Update: USDA VetNet (2004-2009)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In March 2004, USDA VetNet was launched with the intention of providing Pulsed-Field Gel Electrophoresis (PFGE) on Salmonella isolates originating from animals as a complement to the CDC PulseNet program. The objectives of USDA VetNet are to determine PFGE patterns of Salmonella and Campylobacter i...

  18. 77 FR 1052 - Solicitation of Nominations for Members of the USDA Grain Inspection Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-09

    ... Grain Inspection, Packers and Stockyards Administration Solicitation of Nominations for Members of the USDA Grain Inspection Advisory Committee AGENCY: Grain Inspection, Packers and Stockyards Administration, USDA. ACTION: Notice to solicit nominees. SUMMARY: The Department of Agriculture's (USDA)...

  19. 76 FR 86 - Solicitation of Nominations for Members of the USDA Grain Inspection Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-03

    ... Grain Inspection, Packers and Stockyards Administration Solicitation of Nominations for Members of the USDA Grain Inspection Advisory Committee AGENCY: Grain Inspection, Packers and Stockyards Administration, USDA. ACTION: Notice to solicit nominees. SUMMARY: The Department of Agriculture's (USDA)...

  20. 78 FR 74102 - Solicitation of Nominations for Members of the USDA Grain Inspection Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ... Grain Inspection, Packers and Stockyards Administration Solicitation of Nominations for Members of the USDA Grain Inspection Advisory Committee AGENCY: Grain Inspection, Packers and Stockyards Administration, USDA. ACTION: Notice to solicit nominees. SUMMARY: The Department of Agriculture's (USDA)...

  1. Employing microsatellite and SNP markers to track functional mutations and evaluate genetic diversity in the USDA Arachis germplasm collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Peanuts (Arachis hypogaea L.) are nutritious because their seeds typically contain high amounts of oil, protein and other phytochemicals such as folic acid, tocopherol, and antioxidants; therefore, they are an important oil seed crop worldwide. The USDA Plant Genetic Resources Conservation Unit mai...

  2. Influence of cold stress on contents of soluble sugars, vitamin C and free amino acids including gamma-aminobutyric acid (GABA) in spinach (Spinacia oleracea).

    PubMed

    Yoon, Young-Eun; Kuppusamy, Saranya; Cho, Kye Man; Kim, Pil Joo; Kwack, Yong-Bum; Lee, Yong Bok

    2017-01-15

    The contents of soluble sugars (sucrose, fructose, glucose, maltose and raffinose), vitamin C and free amino acids (34 compounds, essential and non-essential) were quantified in open-field and greenhouse-grown spinaches in response to cold stress using liquid chromatography. In general, greenhouse cultivation produced nutritionally high value spinach in a shorter growing period, where the soluble sugars, vitamin C and total amino acids concentrations, including essential were in larger amounts compared to those grown in open-field scenarios. Further, low temperature exposure of spinach during a shorter growth period resulted in the production of spinach with high sucrose, ascorbate, proline, gamma-aminobutyric acid, valine and leucine content, and these constitute the most important energy/nutrient sources. In conclusion, cultivation of spinach in greenhouse at a low temperature (4-7°C) and exposure for a shorter period (7-21days) before harvest is recommended. This strategy will produce a high quality product that people can eat. PMID:27542466

  3. Influence of cold stress on contents of soluble sugars, vitamin C and free amino acids including gamma-aminobutyric acid (GABA) in spinach (Spinacia oleracea).

    PubMed

    Yoon, Young-Eun; Kuppusamy, Saranya; Cho, Kye Man; Kim, Pil Joo; Kwack, Yong-Bum; Lee, Yong Bok

    2017-01-15

    The contents of soluble sugars (sucrose, fructose, glucose, maltose and raffinose), vitamin C and free amino acids (34 compounds, essential and non-essential) were quantified in open-field and greenhouse-grown spinaches in response to cold stress using liquid chromatography. In general, greenhouse cultivation produced nutritionally high value spinach in a shorter growing period, where the soluble sugars, vitamin C and total amino acids concentrations, including essential were in larger amounts compared to those grown in open-field scenarios. Further, low temperature exposure of spinach during a shorter growth period resulted in the production of spinach with high sucrose, ascorbate, proline, gamma-aminobutyric acid, valine and leucine content, and these constitute the most important energy/nutrient sources. In conclusion, cultivation of spinach in greenhouse at a low temperature (4-7°C) and exposure for a shorter period (7-21days) before harvest is recommended. This strategy will produce a high quality product that people can eat.

  4. Impact of hedonic evaluation on consumers' preferences for beef attributes including its enrichment with n-3 and CLA fatty acids.

    PubMed

    Baba, Yasmina; Kallas, Zein; Costa-Font, Montserrat; Gil, José María; Realini, Carolina E

    2016-01-01

    The impact of hedonic evaluation on consumers' preferences for beef attributes was evaluated (origin, animal diet, fat content, color, price) including its enrichment with omega-3 (n-3) and conjugated linoleic acid (CLA) fatty acids. One group of consumers (n=325) received information about n-3 and CLA, while the other group (n=322) received no information. Consumers conducted a Discrete Choice Experiment (DCE), using the recently developed Generalized Multinomial Logit model; followed by a blind hedonic evaluation of beef samples, which were identified after tasting, and finally repeated the DCE. Results showed that hedonic evaluation had a significant impact on consumers' preferences, which were similar after tasting for all consumers, with less emphasis on the fat content, color, and origin attributes and greater emphasis on animal diet. Preference for n-3 enriched beef increased, while preference for CLA enriched beef was still not significant after tasting. The information provided had a significant effect on consumers' beef preferences, but no significant impact on beef liking scores.

  5. Impact of hedonic evaluation on consumers' preferences for beef attributes including its enrichment with n-3 and CLA fatty acids.

    PubMed

    Baba, Yasmina; Kallas, Zein; Costa-Font, Montserrat; Gil, José María; Realini, Carolina E

    2016-01-01

    The impact of hedonic evaluation on consumers' preferences for beef attributes was evaluated (origin, animal diet, fat content, color, price) including its enrichment with omega-3 (n-3) and conjugated linoleic acid (CLA) fatty acids. One group of consumers (n=325) received information about n-3 and CLA, while the other group (n=322) received no information. Consumers conducted a Discrete Choice Experiment (DCE), using the recently developed Generalized Multinomial Logit model; followed by a blind hedonic evaluation of beef samples, which were identified after tasting, and finally repeated the DCE. Results showed that hedonic evaluation had a significant impact on consumers' preferences, which were similar after tasting for all consumers, with less emphasis on the fat content, color, and origin attributes and greater emphasis on animal diet. Preference for n-3 enriched beef increased, while preference for CLA enriched beef was still not significant after tasting. The information provided had a significant effect on consumers' beef preferences, but no significant impact on beef liking scores. PMID:26331961

  6. Understanding the USDA and EPA structure for SBIR Funding

    EPA Science Inventory

    Presentation is part of SBA webinar series on all of the different SBIR agencies. With USDA, this presentation will describe funding opportunities with EPA's SBIR Program. Audience will be small businesses interested in support for their environmental technologies.

  7. 7 CFR 3.79 - Review of USDA records related to the debt.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Review of USDA records related to the debt. 3.79....79 Review of USDA records related to the debt. (a) Notification by employee. An employee who intends to inspect or copy USDA records related to the debt must send a letter to USDA stating his or...

  8. 7 CFR 3.79 - Review of USDA records related to the debt.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Review of USDA records related to the debt. 3.79....79 Review of USDA records related to the debt. (a) Notification by employee. An employee who intends to inspect or copy USDA records related to the debt must send a letter to USDA stating his or...

  9. 7 CFR 3.79 - Review of USDA records related to the debt.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Review of USDA records related to the debt. 3.79....79 Review of USDA records related to the debt. (a) Notification by employee. An employee who intends to inspect or copy USDA records related to the debt must send a letter to USDA stating his or...

  10. 7 CFR 3.79 - Review of USDA records related to the debt.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Review of USDA records related to the debt. 3.79....79 Review of USDA records related to the debt. (a) Notification by employee. An employee who intends to inspect or copy USDA records related to the debt must send a letter to USDA stating his or...

  11. 7 CFR 3.79 - Review of USDA records related to the debt.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Review of USDA records related to the debt. 3.79....79 Review of USDA records related to the debt. (a) Notification by employee. An employee who intends to inspect or copy USDA records related to the debt must send a letter to USDA stating his or...

  12. NIH and USDA funding of dietary supplement research, 1999-2007.

    PubMed

    Regan, Karen S; Wambogo, Edwina A; Haggans, Carol J

    2011-01-01

    Over one-half of U.S. adults use dietary supplements, so federally supported research into the safety and effectiveness of these compounds is important for the health of many Americans. Data collected in the Computer Access to Research on Dietary Supplements database, which compiles federally sponsored dietary supplement-related research, are useful to scientists in determining the type of dietary supplement research that federal agencies are currently funding and where research gaps exist. This article describes the dietary supplement-related research funded by the NIH and the USDA. Between fiscal years 1999 and 2007, the number of research projects and funding for dietary supplement research more than doubled. During that period, NIH funded 6748 dietary supplement-related projects at a cost of $1.9 billion and the USDA funded 2258 projects at a cost of $347 million. The top funded dietary supplement ingredient categories were vitamins and minerals, botanicals, phytochemicals, and fatty acids. Cancer was by far the most frequent health outcome in dietary supplement research funding, nearly double the next closest health outcome category. Other health outcomes with the greatest funding were cellular and molecular mechanisms, cardiovascular health, women's reproductive health, and immune function. The greatest number of dietary supplement research projects are funded by the NIH National Cancer Institute, the NIH National Center for Complementary and Alternative Medicine, the NIH Office of Dietary Supplements, and the USDA Agricultural Research Service.

  13. Isolation and identification of microorganisms including lactic acid bacteria and their use in microbial deacidification of wines from domestic vineyards.

    PubMed

    Drozdz, Iwona; Makarewicz, Malgorzata; Tuszyński, Tadeusz

    2013-01-01

    The aim of this study was to identify various bacteria isolated from grapes and their wines. Additionally we investigated the capacity of lactic acid bacteria for microbiological deacidification of wines produced in Poland. We have identified Oenococcus oeni, Lactobacillus acidophilus and Lactobacillus delbrueckii. During the microbial deacidification process, we observed decreases of total acidity and increases of volatile acidity, with statistically significant changes noted for O. oeni in Marechal Foch and Seyval Blanc, and for Lb. acidophilus in Frontenac. On the other hand, a statistically significant increase in pH was observed in Marechal Foch and Seyval Blanc following deacidification by O. oeni.

  14. Separation of fatty acids or methyl esters including positional and geometric isomers by alumina argentation thin-layer chromatography.

    PubMed

    Breuer, B; Stuhlfauth, T; Fock, H P

    1987-07-01

    This paper describes novel and rapid thin-layer chromatography procedures for the analysis of fatty acids and methyl esters using silver-impregnated alumina sheets. These techniques are known in most laboratories, and the equipment is readily available. The fatty acid method allows a separation of petroselinic (C18:1 delta 6c), oleic (C18:1 delta 9c), elaidic (C18:1 delta 9t), erucic (C22:1 delta 13c), and brassidic acids (C22:1 delta 13t), and the methyl ester method gives an excellent resolution with respect to the number, configuration, and position of the unsaturated centers. Sufficient separation for the subsequent ozonolysis and chromatographic quantification of isomeric C18 and C22 fatty acid methyl esters is obtained with both methods.

  15. Fatty acid composition, including CLA's isomers and cholesterol content of m. longissimus lumborum and m. semimebranosus of Katahdin, Suffolk, Katahdin x Suffolk, and Suffolk x Katahdin lambs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Lipids in meat products have important human health implications. Muscle tissues from Katahdin (KK), Suffolk (SS), Katahdin x Suffolk (KS), and Suffolk x Katahdin (SS) lambs were analyzed to determine the effect of breed-type on muscle fatty acid composition, including conjugated linoleic acid (CLA)...

  16. Rapid identification of triterpenoid sulfates and hydroxy fatty acids including two new constituents from Tydemania expeditionis by LC-MS

    PubMed Central

    Zhang, Jian-Long; Kubanek, Julia; Hay, Mark E.; Aalbersberg, William; Ye, Wen-Cai; Jiang, Ren-Wang

    2011-01-01

    Tydemania expeditionis Weber-van Bosse (Udoteaceae) is a weakly calcified green alga. In the present paper, liquid chromatography coupled with photodiode array detection and electrospray mass spectrometry was developed to identify the fingerprint components. A total of four triterpenoid sulfates and three hydroxy fatty acids in the ethyl acetate fraction of the crude extract were structurally characterized on the basis of retention time, online UV spectrum and mass fragmentation pattern. Furthermore, detailed LC-MS analysis revealed two new hydroxy fatty acids, which were then prepared and characterized by extensive NMR analyses. The proposed method provides a scientific and technical platform for the rapid identification of triterpenoid sulfates and hydroxy fatty acids in similar marine algae and terrestrial plants. PMID:21915955

  17. Breeding citrus for HLB resistance at the USDA/ARS U.S. Horticultural Research Laboratory, Ft. Pierce, Florida

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Citrus breeding has been conducted by the USDA since 1893. The initial objectives included improved disease-resistance, cold hardiness, and easy peeling fruit, which are still important breeding objectives today. The emergence of huanglongbing (HLB) in the US has propelled the development of HLB r...

  18. Genotypic and phenotypic characterization of genetic differentiation and diversity in the USDA rice mini-core collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A rice mini-core collection consisting of 217 accessions has been developed to represent the USDA core and whole collections that include 1,794 and 18,709 accessions, respectively. To improve the efficiency of mining valuable genes and broadening the genetic diversity in breeding, genetic structure ...

  19. Fatty acid composition of ruminal digesta and longissimus muscle from lambs fed silage mixtures including red clover, sainfoin, and timothy.

    PubMed

    Campidonico, L; Toral, P G; Priolo, A; Luciano, G; Valenti, B; Hervás, G; Frutos, P; Copani, G; Ginane, C; Niderkorn, V

    2016-04-01

    This work investigated the effects of feeding silage mixtures of a plant containing polyphenol oxidase (PPO; red clover [; RC]), a plant containing tannins (sainfoin [; SF]), and a grass species not containing these compounds (timothy [; T]) on ruminal and intramuscular (i.m.) fatty acids of lambs. Forty 4-mo-old castrated male Romane lambs, divided into 5 groups, received 1 of the following silages: 1) T (100%), 2) a binary mixture of timothy and tannin-containing sainfoin ( cv. Perly; 50:50 [T-SF]), 3) a binary mixture of timothy and PPO-containing red clover ( cv. Mervius; 50:50 [T-RC]), 4) a ternary mixture of timothy, sainfoin, and red clover containing both tannins and PPO (50:25:25, respectively [T-SF-RC]), and 5) a binary mixture of tannin-containing sainfoin and PPO-containing red clover (50:50 [SF-RC]). In the rumen digesta, the partial or total replacement of T with forage legumes was associated with greater concentrations of PUFA ( < 0.001) and 1esser concentrations of MUFA ( < 0.001). The inclusion of forage legumes in the silage favored the accumulation of 18:3 -3 ( < 0.001), with the greatest concentrations being observed in SF-RC. This latter diet also led to the greatest percentage of 18:2 -6 ( < 0.001). Forage legumes decreased the -11 18:1 to 30% of T in rumen digesta ( < 0.001). Forage legumes decreased the total concentration of branched-chain fatty acids in the rumen digesta (on average, -28%; < 0.001), this effect being less marked (-17%; = 0.014) in T-RC in comparison with T. The dietary treatment tended to affect the proportion of MUFA ( = 0.081) and of PUFA ( = 0.079) in the i.m. fat of the LM, respectively, at the highest and lowest numerical value in the T group. The sum of -3 fatty acids was less in the T and T-SF groups compared with the mixture of legumes without T (SF-RC; < 0.001 and < 0.008, respectively). The latter group had also a lesser -6-to--3 ratio than the T-SF group ( = 0.01). -11 18:1 was greater ( < 0.03) in lambs given T

  20. Fatty acid composition of ruminal digesta and longissimus muscle from lambs fed silage mixtures including red clover, sainfoin, and timothy.

    PubMed

    Campidonico, L; Toral, P G; Priolo, A; Luciano, G; Valenti, B; Hervás, G; Frutos, P; Copani, G; Ginane, C; Niderkorn, V

    2016-04-01

    This work investigated the effects of feeding silage mixtures of a plant containing polyphenol oxidase (PPO; red clover [; RC]), a plant containing tannins (sainfoin [; SF]), and a grass species not containing these compounds (timothy [; T]) on ruminal and intramuscular (i.m.) fatty acids of lambs. Forty 4-mo-old castrated male Romane lambs, divided into 5 groups, received 1 of the following silages: 1) T (100%), 2) a binary mixture of timothy and tannin-containing sainfoin ( cv. Perly; 50:50 [T-SF]), 3) a binary mixture of timothy and PPO-containing red clover ( cv. Mervius; 50:50 [T-RC]), 4) a ternary mixture of timothy, sainfoin, and red clover containing both tannins and PPO (50:25:25, respectively [T-SF-RC]), and 5) a binary mixture of tannin-containing sainfoin and PPO-containing red clover (50:50 [SF-RC]). In the rumen digesta, the partial or total replacement of T with forage legumes was associated with greater concentrations of PUFA ( < 0.001) and 1esser concentrations of MUFA ( < 0.001). The inclusion of forage legumes in the silage favored the accumulation of 18:3 -3 ( < 0.001), with the greatest concentrations being observed in SF-RC. This latter diet also led to the greatest percentage of 18:2 -6 ( < 0.001). Forage legumes decreased the -11 18:1 to 30% of T in rumen digesta ( < 0.001). Forage legumes decreased the total concentration of branched-chain fatty acids in the rumen digesta (on average, -28%; < 0.001), this effect being less marked (-17%; = 0.014) in T-RC in comparison with T. The dietary treatment tended to affect the proportion of MUFA ( = 0.081) and of PUFA ( = 0.079) in the i.m. fat of the LM, respectively, at the highest and lowest numerical value in the T group. The sum of -3 fatty acids was less in the T and T-SF groups compared with the mixture of legumes without T (SF-RC; < 0.001 and < 0.008, respectively). The latter group had also a lesser -6-to--3 ratio than the T-SF group ( = 0.01). -11 18:1 was greater ( < 0.03) in lambs given T

  1. Liquid chromatographic resolution of amino acid esters of acyclovir including racemic valacyclovir on crown ether-based chiral stationary phases.

    PubMed

    Ahn, Seong Ae; Hyun, Myung Ho

    2015-03-01

    Valacyclovir, a potential prodrug for the treatment of patients with herpes simplex and herpes zoster, and its analogs were resolved on two chiral stationary phases (CSPs) based on (3,3'-diphenyl-1,1'-binaphthyl)-20-crown-6 covalently bonded to silica gel. In order to find out an appropriate mobile phase condition, various mobile phases consisting of various organic modifiers in water containing various acidic modifiers were applied to the resolution of valacyclovir and its analogs. When 30% acetonitrile in water containing any of 0.05 M, 0.10 M, or 0.15 M perchloric acid was used as a mobile phase, valacyclovir and its analogs were resolved quite well on the two CSPs with the separation factors (α) in the range of 2.49 ~ 6.35 and resolutions (RS ) in the range of 2.95 ~ 12.21. Between the two CSPs, the CSP containing residual silanol protecting n-octyl groups on the silica surface was found to be better than the CSP containing residual silanol groups.

  2. DMPS-arsenic challenge test. II. Modulation of arsenic species, including monomethylarsonous acid (MMA(III)), excreted in human urine.

    PubMed

    Aposhian, H V; Zheng, B; Aposhian, M M; Le, X C; Cebrian, M E; Cullen, W; Zakharyan, R A; Ma, M; Dart, R C; Cheng, Z; Andrewes, P; Yip, L; O'Malley, G F; Maiorino, R M; Van Voorhies, W; Healy, S M; Titcomb, A

    2000-05-15

    The administration of sodium 2,3-dimercapto-1-propane sulfonate (DMPS) to humans chronically exposed to inorganic arsenic in their drinking water resulted in the increased urinary excretion of arsenic, the appearance and identification of monomethylarsonous acid (MMA(III)) in their urine, and a large decrease in the concentration and percentage of urinary dimethylarsinic acid (DMA). This is the first time that MMA(III) has been detected in the urine. In vitro biochemical experiments were then designed and performed to understand the urinary appearance of MMA(III) and decrease of DMA. The DMPS-MMA(III) complex was not active as a substrate for the MMA(III) methyltransferase. The experimental results support the hypothesis that DMPS competes with endogenous ligands for MMA(III), forming a DMPS-MMA complex that is readily excreted in the urine and points out the need for studying the biochemical toxicology of MMA(III). It should be emphasized that MMA(III) was excreted in the urine only after DMPS administration. The results of these studies raise many questions about the potential central role of MMA(III) in the toxicity of inorganic arsenic and to the potential involvement of MMA(III) in the little-understood etiology of hyperkeratosis, hyperpigmentation, and cancer that can result from chronic inorganic arsenic exposure.

  3. Liquid chromatographic resolution of amino acid esters of acyclovir including racemic valacyclovir on crown ether-based chiral stationary phases.

    PubMed

    Ahn, Seong Ae; Hyun, Myung Ho

    2015-03-01

    Valacyclovir, a potential prodrug for the treatment of patients with herpes simplex and herpes zoster, and its analogs were resolved on two chiral stationary phases (CSPs) based on (3,3'-diphenyl-1,1'-binaphthyl)-20-crown-6 covalently bonded to silica gel. In order to find out an appropriate mobile phase condition, various mobile phases consisting of various organic modifiers in water containing various acidic modifiers were applied to the resolution of valacyclovir and its analogs. When 30% acetonitrile in water containing any of 0.05 M, 0.10 M, or 0.15 M perchloric acid was used as a mobile phase, valacyclovir and its analogs were resolved quite well on the two CSPs with the separation factors (α) in the range of 2.49 ~ 6.35 and resolutions (RS ) in the range of 2.95 ~ 12.21. Between the two CSPs, the CSP containing residual silanol protecting n-octyl groups on the silica surface was found to be better than the CSP containing residual silanol groups. PMID:25626672

  4. Final corrective action study for the former CCC/USDA facility in Ramona, Kansas.

    SciTech Connect

    LaFreniere, L. M.

    2011-04-20

    Past operations at a grain storage facility formerly leased and operated by the Commodity Credit Corporation of the U.S. Department of Agriculture (CCC/USDA) in Ramona, Kansas, resulted in low concentrations of carbon tetrachloride in groundwater that slightly exceed the regulatory standard in only one location. As requested by the Kansas Department of Health and Environment, the CCC/USDA has prepared a Corrective Action Study (CAS) for the facility. The CAS examines corrective actions to address groundwater impacted by the former CCC/USDA facility but not releases caused by other potential groundwater contamination sources in Ramona. Four remedial alternatives were considered in the CAS. The recommended remedial alternative in the CAS consists of Environmental Use Control to prevent the inadvertent use of groundwater as a water supply source, coupled with groundwater monitoring to verify the continued natural improvement in groundwater quality. The Commodity Credit Corporation of the U.S. Department of Agriculture (CCC/USDA) has directed Argonne National Laboratory to prepare a Corrective Action Study (CAS), consistent with guidance from the Kansas Department of Health and Environment (KDHE 2001a), for the CCC/USDA grain storage facility formerly located in Ramona, Kansas. This effort is pursuant to a KDHE (2007a) request. Although carbon tetrachloride levels at the Ramona site are low, they remain above the Kansas Tier 2 risk-based screening level (RBSL) and the U.S. Environmental Protection Agency (EPA) maximum contaminant level (MCL) of 5 {micro}g/L (Kansas 2003, 2004). In its request for the CAS, the KDHE (2007a) stated that, because of these levels, risk is associated with potential future exposure to contaminated groundwater. The KDHE therefore determined that additional measures are warranted to limit future use of the property and/or exposure to contaminated media as part of site closure. The KDHE further requested comparison of at least two corrective

  5. Growth and development of the arborescent cactus Stenocereus queretaroensis in a subtropical semiarid environment, including effects of gibberellic acid.

    PubMed

    Pimienta, Eulogio; Hernandez, Gerardo; Domingues, Alejandro; Nobel, Park S.

    1998-01-01

    In Stenocereus queretaroensis (Weber) Buxbaum, an arborescent cactus cultivated in Jalisco, Mexico, for its fruits but studied here in wild populations, stem extension occurred in the autumn at the beginning of the dry season, flowering and fruiting occurred in the spring at the end of the dry season, and new roots grew in the summer during the wet season. The asynchrony of vegetative and reproductive growth reduces competitive sink effects, which may be advantageous for wild populations growing in infertile rocky soils. Seasonal patterns of sugars in the roots and especially the stems of S. queretaroensis were closely related to the main phenological stages, becoming lower in concentration during periods of major stem extension. Cessation of stem extension occurred in 100-year-old plants for which injection of GA(3) reinitiated such growth. Isolated chlorenchyma cylinders had maximum extension in a bathing solution containing 0.1 &mgr;M gibberellic acid.

  6. Skin aging and photoaging alter fatty acids composition, including 11,14,17-eicosatrienoic acid, in the epidermis of human skin.

    PubMed

    Kim, Eun Ju; Kim, Min-Kyoung; Jin, Xing-Ji; Oh, Jang-Hee; Kim, Ji Eun; Chung, Jin Ho

    2010-06-01

    We investigated the alterations of major fatty acid components in epidermis by natural aging and photoaging processes, and by acute ultraviolet (UV) irradiation in human skin. Interestingly, we found that 11,14,17-eicosatrienoic acid (ETA), which is one of the omega-3 polyunsaturated acids, was significantly increased in photoaged human epidermis in vivo and also in the acutely UV-irradiated human skin in vivo, while it was significantly decreased in intrinsically aged human epidermis. The increased ETA content in the epidermis of photoaged human skin and acute UV-irradiated human skin is associated with enhanced expression of human elongase 1 and calcium-independent phosphodiesterase A(2). We demonstrated that ETA inhibited matrix metalloproteinase (MMP)-1 expression after UV-irradiation, and that inhibition of ETA synthesis using EPTC and NA-TCA, which are elongase inhibitors, increased MMP-1 expression. Therefore, our results suggest that the UV increases the ETA levels, which may have a photoprotective effect in the human skin.

  7. Identifying the future needs for long-term USDA efforts in agricultural animal genomics.

    PubMed

    Green, R D; Qureshi, M A; Long, J A; Burfening, P J; Hamernik, D L

    2007-02-10

    Agricultural animal research has been immensely successful over the past century in developing technology and methodologies that have dramatically enhanced production efficiency of the beef, dairy, swine, poultry, sheep, and aquaculture industries. In the past two decades, molecular biology has changed the face of agricultural animal research, primarily in the arena of genomics and the relatively new offshoot areas of functional genomics, proteomics, transcriptomics, metabolomics and metagenomics. Publication of genetic and physical genome maps in the past 15 years has given rise to the possibility of being able finally to understand the molecular nature of the genetic component of phenotypic variation. While quantitative geneticists have been remarkably successful in improving production traits, genomic technology holds potential for being able to lead to more accurate and rapid animal improvement, especially for phenotypic traits that are difficult to measure.Recently, the agricultural research community has been able to capitalize on the infrastructure built by the human genome project by sequencing two of the major livestock genomes (Gallus domesticus and Bos Taurus). The 2005 calendar year is truly unprecedented in the history of agricultural animal research since draft genome sequences were completed for chickens and cattle. In addition, sequencing the swine and equine genome was initiated in early 2006. We now have in place a powerful toolbox for understanding the genetic variation underlying economically important and complex phenotypes. Over the past few years, new challenges have emerged for animal agriculture. Enhancements in production efficiency have not come without some negative side effects on animal well-being and longevity in production environments, including losses in reproductive efficiency, increased stress susceptibility, increased animal waste issues, and increased susceptibility to animal metabolic and infectious diseases. When considered

  8. 7 CFR 170.13 - What are the operating guidelines for the USDA Farmers Market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...,” 41 CFR 20.3. ... 7 Agriculture 3 2014-01-01 2014-01-01 false What are the operating guidelines for the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA...

  9. 7 CFR 170.13 - What are the operating guidelines for the USDA Farmers Market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...,” 41 CFR 20.3. ... 7 Agriculture 3 2012-01-01 2012-01-01 false What are the operating guidelines for the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA...

  10. 7 CFR 170.13 - What are the operating guidelines for the USDA Farmers Market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...,” 41 CFR 20.3. ... 7 Agriculture 3 2010-01-01 2010-01-01 false What are the operating guidelines for the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA...

  11. 7 CFR 170.13 - What are the operating guidelines for the USDA Farmers Market?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...,” 41 CFR 20.3. ... 7 Agriculture 3 2011-01-01 2011-01-01 false What are the operating guidelines for the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA...

  12. Chemical Genetics Uncovers Novel Inhibitors of Lignification, Including p-Iodobenzoic Acid Targeting CINNAMATE-4-HYDROXYLASE1[OPEN

    PubMed Central

    Van de Wouwer, Dorien; Decou, Raphaël; Audenaert, Dominique; Nguyen, Long

    2016-01-01

    Plant secondary-thickened cell walls are characterized by the presence of lignin, a recalcitrant and hydrophobic polymer that provides mechanical strength and ensures long-distance water transport. Exactly the recalcitrance and hydrophobicity of lignin put a burden on the industrial processing efficiency of lignocellulosic biomass. Both forward and reverse genetic strategies have been used intensively to unravel the molecular mechanism of lignin deposition. As an alternative strategy, we introduce here a forward chemical genetic approach to find candidate inhibitors of lignification. A high-throughput assay to assess lignification in Arabidopsis (Arabidopsis thaliana) seedlings was developed and used to screen a 10-k library of structurally diverse, synthetic molecules. Of the 73 compounds that reduced lignin deposition, 39 that had a major impact were retained and classified into five clusters based on the shift they induced in the phenolic profile of Arabidopsis seedlings. One representative compound of each cluster was selected for further lignin-specific assays, leading to the identification of an aromatic compound that is processed in the plant into two fragments, both having inhibitory activity against lignification. One fragment, p-iodobenzoic acid, was further characterized as a new inhibitor of CINNAMATE 4-HYDROXYLASE, a key enzyme of the phenylpropanoid pathway synthesizing the building blocks of the lignin polymer. As such, we provide proof of concept of this chemical biology approach to screen for inhibitors of lignification and present a broad array of putative inhibitors of lignin deposition for further characterization. PMID:27485881

  13. 7 CFR 983.10 - Department or USDA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Department or USDA. 983.10 Section 983.10 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE PISTACHIOS GROWN IN...

  14. Pulsed electric field (PEF)research at USDA, ARS, ERRC

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This article summarizes the effects of pulsed electric fields on the microbiological safety and quality aspects of various liquid food matrices, obtained at USDA, ARS, Eastern Regional Research Center under CRIS Project No. 1935-41420-013-00D, Processing Intervention Technologies for Enhancing the S...

  15. Postharvest treatment research at USDA-ARS: stored product fumigation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The overall goal of this USDA-ARS research is to ensure the protection and quality of stored product foodstuffs. The results of this research directly enhance production, distribution, and safety of foodstuffs, promote and retain access of United States-grown crops to domestic and foreign markets, a...

  16. Introductory Soil Science Exercises Using USDA Web Soil Survey

    ERIC Educational Resources Information Center

    Post, Christopher J.; Mikhailova, Elena; McWhorter, Christopher M.

    2007-01-01

    The USDA, Natural Resource Conservation Service (NRCS) Web Soil Survey is a valuable teaching tool for soil science education. By incorporating the Web Soil Survey into an undergraduate-level course, students are able to use the most detailed digital soil survey information without the steep learning curve associated with geographic information…

  17. USDA National Nutrient Database for Standard Reference, Release 25

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA National Nutrient Database for Standard Reference, Release 25(SR25)contains data for over 8,100 food items for up to 146 food components. It replaces the previous release, SR24, issued in September 2011. Data in SR25 supersede values in the printed handbooks and previous electronic releas...

  18. USDA develops a database for flavonoids to assess dietary intakes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The beneficial health effects of dietary flavonoids continue to interest the scientific community in associating the flavonoid intakes and certain chronic diseases. Scientists at the Nutrient Data Laboratory (NDL) and the Food Surveys Research Group (FSRG), USDA planned a study of the intakes of fl...

  19. USDA Research in Support of Deployed Military Troops

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The US Department of Agriculture has a long history of collaborating with the US military to conduct research in support of war efforts. The predecessor laboratory of the current USDA-ARS Center for Medical, Agricultural and Veterinary Entomology (CMAVE) located in Gainesville, Florida has a history...

  20. USDA Research on New Strategies for Controlling Aedes aegypti.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    USDA researchers are currently studying new methods to control Aedes aegypti. One involves molecular pesticides which target critical genes/proteins (such as inhibitors of apoptosis proteins, IAPs) in mosquitoes using RNA interference (RNAi). RNAi constructs are evaluated in vivo in adult mosquito...

  1. USDA National Nutrient Database for Standard Reference, release 28

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA National Nutrient Database for Standard Reference, Release 28 contains data for nearly 8,800 food items for up to 150 food components. SR28 replaces the previous release, SR27, originally issued in August 2014. Data in SR28 supersede values in the printed handbooks and previous electronic...

  2. USDA-ARS and EMBRAPA Scientists Build Bridges With Strawberries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    At the '3rd Simposio Nacional do Morango'and '2nd Encontro de Pequenas Frutas e Frutas Nativas do Mercosul' at Pelotas, Rio Grande Do Sul, Brazil, held 7 - 10 November 2006, I was invited to describe strawberry breeding in the United States and also my own USDA-ARS strawberry breeding program at Bel...

  3. USDA National Nutrient Database for Standard Reference, Release 24

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Nutrient Database for Standard Reference, Release 24 contains data for over 7,900 food items for up to 146 food components. It replaces the previous release, SR23, issued in September 2010. Data in SR24 supersede values in the printed Handbooks and previous electronic releases of the databa...

  4. USDA National Nutrient Database for Standard Reference, Release 23

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Nutrient Database for Standard Reference, Release 23 contains data for over 7,600 food items for up to 146 food components when a complete profile is available for a food item. It replaces the previous release, SR22, issued in September 2009. Data in SR23 supersede values in the printed h...

  5. USDA National Nutrient Database for Standard Reference, Release 22

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Nutrient Database for Standard Reference, Release 22 contains data for over 7,500 food items for up to 143 food components when a complete profile is available for a food item. It replaces the previous release, SR21, issued in September 2008. Data in SR22 supersede values in the printed Han...

  6. USDA National Nutrient Database for Standard Reference, Release 21

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Nutrient Database for Standard Reference, Release 21 contains data for 7,414 food items for up to 140 food components when a complete profile is available for a food item. It replaces the previous release, SR20, issued in September 2007. Data in SR21 supersede values in the printed Handbook...

  7. The creation and role of the USDA biomass research centers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Five USDA Biomass Research Centers were created to facilitate coordinated research to enhance the establishment of a sustainable feedstock production for bio-based renewable energy in the United States. Scientists and staff of the Agriculture Research Service (ARS) and Forest Service (FS) withi...

  8. USDA updates nutrient values for fast food pizza

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Consumption of quick service pizza has increased as Americans are spending more on food away from home. Pizza is consistently a primary Key Food in the USDA National Food and Nutrient Analysis Program (NFNAP) because it is a contributor of more than 14 nutrients of public health significance to the...

  9. Hungry for Nutrient Data? Navigating the USDA Nutrient Database

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA National Nutrient Database for Standard Reference (SR) is the major source of food composition data in the United States, providing the foundation for most food composition databases in the public and private sectors. Most nutrition professionals are familiar with the basics of the SR onlin...

  10. Retinoic Acid Induced 1, RAI1: A Dosage Sensitive Gene Related to Neurobehavioral Alterations Including Autistic Behavior

    PubMed Central

    Carmona-Mora, Paulina; Walz, Katherina

    2010-01-01

    Genomic structural changes, such as gene Copy Number Variations (CNVs) are extremely abundant in the human genome. An enormous effort is currently ongoing to recognize and catalogue human CNVs and their associations with abnormal phenotypic outcomes. Recently, several reports related neuropsychiatric diseases (i.e. autism spectrum disorders, schizophrenia, mental retardation, behavioral problems, epilepsy) with specific CNV. Moreover, for some conditions, both the deletion and duplication of the same genomic segment are related to the phenotype. Syndromes associated with CNVs (microdeletion and microduplication) have long been known to display specific neurobehavioral traits. It is important to note that not every gene is susceptible to gene dosage changes and there are only a few dosage sensitive genes. Smith-Magenis (SMS) and Potocki-Lupski (PTLS) syndromes are associated with a reciprocal microdeletion and microduplication within chromosome 17p11.2. in humans. The dosage sensitive gene responsible for most phenotypes in SMS has been identified: the Retinoic Acid Induced 1 (RAI1). Studies on mouse models and humans suggest that RAI1 is likely the dosage sensitive gene responsible for clinical features in PTLS. In addition, the human RAI1 gene has been implicated in several neurobehavioral traits as spinocerebellar ataxia (SCA2), schizophrenia and non syndromic autism. In this review we discuss the evidence of RAI1 as a dosage sensitive gene, its relationship with different neurobehavioral traits, gene structure and mutations, and what is known about its molecular and cellular function, as a first step in the elucidation of the mechanisms that relate dosage sensitive genes with abnormal neurobehavioral outcomes. PMID:21629438

  11. 7 CFR 170.2 - Is the USDA Farmers Market a producer-only market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Is the USDA Farmers Market a producer-only market? 170...) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.2 Is the USDA Farmers Market a producer-only market? Yes. A producer-only market is one that does not...

  12. 7 CFR 170.2 - Is the USDA Farmers Market a producer-only market?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Is the USDA Farmers Market a producer-only market? 170...) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.2 Is the USDA Farmers Market a producer-only market? Yes. A producer-only market is one that does not...

  13. 7 CFR 1735.9 - USDA Rural Development State Director notification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 11 2014-01-01 2014-01-01 false USDA Rural Development State Director notification....9 USDA Rural Development State Director notification. Applicants shall complete a notification form which will be a public document that the RUS provides to USDA Rural Development State Directors...

  14. 7 CFR 58.122 - Approved plants under USDA inspection and grading service.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Purpose § 58.122 Approved plants under USDA inspection and grading service. (a... 7 Agriculture 3 2010-01-01 2010-01-01 false Approved plants under USDA inspection and...

  15. 7 CFR 900.40 - Written testimony and USDA data request requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Written testimony and USDA data request requirements... and Nut Marketing Agreements and Marketing Orders § 900.40 Written testimony and USDA data request... testimony, if prepared as an exhibit, and any other exhibits available to USDA before testimony is given...

  16. 7 CFR 170.14 - What circumstances will prevent participation in the USDA Farmers Market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... USDA Farmers Market? 170.14 Section 170.14 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.14 What circumstances will prevent participation in the USDA Farmers Market?...

  17. 7 CFR 170.14 - What circumstances will prevent participation in the USDA Farmers Market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... USDA Farmers Market? 170.14 Section 170.14 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.14 What circumstances will prevent participation in the USDA Farmers Market?...

  18. 7 CFR 170.6 - How are potential market participants identified for the USDA Farmers Market?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... the USDA Farmers Market? 170.6 Section 170.6 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.6 How are potential market participants identified for the USDA Farmers...

  19. 7 CFR 3022.4 - USDA Panel to determine appropriateness of research misconduct policy.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false USDA Panel to determine appropriateness of research...) OFFICE OF THE CHIEF FINANCIAL OFFICER, DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.4 USDA Panel to determine appropriateness...

  20. 7 CFR 900.26 - Requesting USDA data for use at an amendatory hearing.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Requesting USDA data for use at an amendatory hearing... Marketing Agreements and Marketing Orders § 900.26 Requesting USDA data for use at an amendatory hearing. Requests for preparation of USDA data to be used at a Federal milk marketing agreement or order...

  1. 7 CFR 170.5 - Is there a fee to participate in the USDA Farmers Market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Is there a fee to participate in the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.5 Is there a fee to participate in the USDA Farmers Market? No, there are no fees charged...

  2. 7 CFR 170.5 - Is there a fee to participate in the USDA Farmers Market?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Is there a fee to participate in the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.5 Is there a fee to participate in the USDA Farmers Market? No, there are no fees charged...

  3. 7 CFR 3022.6 - Notification of USDA of allegations of research misconduct.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Notification of USDA of allegations of research... OF THE CHIEF FINANCIAL OFFICER, DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.6 Notification of USDA of allegations of...

  4. 7 CFR 170.6 - How are potential market participants identified for the USDA Farmers Market?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... the USDA Farmers Market? 170.6 Section 170.6 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.6 How are potential market participants identified for the USDA Farmers...

  5. 7 CFR 900.26 - Requesting USDA data for use at an amendatory hearing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Requesting USDA data for use at an amendatory hearing... Marketing Agreements and Marketing Orders § 900.26 Requesting USDA data for use at an amendatory hearing. Requests for preparation of USDA data to be used at a Federal milk marketing agreement or order...

  6. 7 CFR 3022.6 - Notification of USDA of allegations of research misconduct.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false Notification of USDA of allegations of research... OF THE CHIEF FINANCIAL OFFICER, DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.6 Notification of USDA of allegations of...

  7. 7 CFR 900.40 - Written testimony and USDA data request requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Written testimony and USDA data request requirements... and Nut Marketing Agreements and Marketing Orders § 900.40 Written testimony and USDA data request... testimony, if prepared as an exhibit, and any other exhibits available to USDA before testimony is given...

  8. 7 CFR 170.14 - What circumstances will prevent participation in the USDA Farmers Market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... USDA Farmers Market? 170.14 Section 170.14 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.14 What circumstances will prevent participation in the USDA Farmers Market?...

  9. 7 CFR 3022.6 - Notification of USDA of allegations of research misconduct.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 15 2012-01-01 2012-01-01 false Notification of USDA of allegations of research... OF THE CHIEF FINANCIAL OFFICER, DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.6 Notification of USDA of allegations of...

  10. 7 CFR 58.122 - Approved plants under USDA inspection and grading service.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Approved plants under USDA inspection and grading... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Purpose § 58.122 Approved plants under USDA inspection and grading service....

  11. 7 CFR 170.6 - How are potential market participants identified for the USDA Farmers Market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... the USDA Farmers Market? 170.6 Section 170.6 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.6 How are potential market participants identified for the USDA Farmers...

  12. 7 CFR 170.14 - What circumstances will prevent participation in the USDA Farmers Market?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... USDA Farmers Market? 170.14 Section 170.14 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.14 What circumstances will prevent participation in the USDA Farmers Market?...

  13. 7 CFR 4290.509 - Employment of USDA or SBA officials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false Employment of USDA or SBA officials. 4290.509 Section... (âRBICâ) PROGRAM Managing the Operations of a RBIC General Requirements § 4290.509 Employment of USDA... of SBA or USDA within one year before such date; and (2) In that capacity, occupied a position...

  14. 7 CFR 658.7 - USDA assistance with Federal agencies' reviews of policies and procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 6 2014-01-01 2014-01-01 false USDA assistance with Federal agencies' reviews of... PROTECTION POLICY ACT § 658.7 USDA assistance with Federal agencies' reviews of policies and procedures. (a... the purpose and policy of the Act. (c) USDA will provide certain assistance to other Federal...

  15. 7 CFR 658.7 - USDA assistance with Federal agencies' reviews of policies and procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false USDA assistance with Federal agencies' reviews of... PROTECTION POLICY ACT § 658.7 USDA assistance with Federal agencies' reviews of policies and procedures. (a... the purpose and policy of the Act. (c) USDA will provide certain assistance to other Federal...

  16. 7 CFR 170.2 - Is the USDA Farmers Market a producer-only market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Is the USDA Farmers Market a producer-only market? 170...) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.2 Is the USDA Farmers Market a producer-only market? Yes. A producer-only market is one that does not...

  17. 7 CFR 3022.6 - Notification of USDA of allegations of research misconduct.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false Notification of USDA of allegations of research... OF THE CHIEF FINANCIAL OFFICER, DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.6 Notification of USDA of allegations of...

  18. 7 CFR 900.40 - Written testimony and USDA data request requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Written testimony and USDA data request requirements... and Nut Marketing Agreements and Marketing Orders § 900.40 Written testimony and USDA data request... testimony, if prepared as an exhibit, and any other exhibits available to USDA before testimony is given...

  19. 7 CFR 3022.4 - USDA Panel to determine appropriateness of research misconduct policy.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 15 2012-01-01 2012-01-01 false USDA Panel to determine appropriateness of research...) OFFICE OF THE CHIEF FINANCIAL OFFICER, DEPARTMENT OF AGRICULTURE RESEARCH INSTITUTIONS CONDUCTING USDA-FUNDED EXTRAMURAL RESEARCH; RESEARCH MISCONDUCT § 3022.4 USDA Panel to determine appropriateness...

  20. 7 CFR 170.5 - Is there a fee to participate in the USDA Farmers Market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Is there a fee to participate in the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.5 Is there a fee to participate in the USDA Farmers Market? No, there are no fees charged...

  1. 7 CFR 58.122 - Approved plants under USDA inspection and grading service.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Approved plants under USDA inspection and grading... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Purpose § 58.122 Approved plants under USDA inspection and grading service....

  2. 7 CFR 58.122 - Approved plants under USDA inspection and grading service.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Approved plants under USDA inspection and grading... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Purpose § 58.122 Approved plants under USDA inspection and grading service....

  3. 7 CFR 170.5 - Is there a fee to participate in the USDA Farmers Market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Is there a fee to participate in the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.5 Is there a fee to participate in the USDA Farmers Market? No, there are no fees charged...

  4. 7 CFR 170.14 - What circumstances will prevent participation in the USDA Farmers Market?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... USDA Farmers Market? 170.14 Section 170.14 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.14 What circumstances will prevent participation in the USDA Farmers Market?...

  5. 7 CFR 658.7 - USDA assistance with Federal agencies' reviews of policies and procedures.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 6 2013-01-01 2013-01-01 false USDA assistance with Federal agencies' reviews of... PROTECTION POLICY ACT § 658.7 USDA assistance with Federal agencies' reviews of policies and procedures. (a... the purpose and policy of the Act. (c) USDA will provide certain assistance to other Federal...

  6. 7 CFR 170.6 - How are potential market participants identified for the USDA Farmers Market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... the USDA Farmers Market? 170.6 Section 170.6 Agriculture Regulations of the Department of Agriculture... AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.6 How are potential market participants identified for the USDA Farmers...

  7. 7 CFR 58.122 - Approved plants under USDA inspection and grading service.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Approved plants under USDA inspection and grading... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Purpose § 58.122 Approved plants under USDA inspection and grading service....

  8. 7 CFR 900.26 - Requesting USDA data for use at an amendatory hearing.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Requesting USDA data for use at an amendatory hearing... Marketing Agreements and Marketing Orders § 900.26 Requesting USDA data for use at an amendatory hearing. Requests for preparation of USDA data to be used at a Federal milk marketing agreement or order...

  9. 7 CFR 4290.509 - Employment of USDA or SBA officials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false Employment of USDA or SBA officials. 4290.509 Section... (âRBICâ) PROGRAM Managing the Operations of a RBIC General Requirements § 4290.509 Employment of USDA... of SBA or USDA within one year before such date; and (2) In that capacity, occupied a position...

  10. 7 CFR 900.26 - Requesting USDA data for use at an amendatory hearing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Requesting USDA data for use at an amendatory hearing... Marketing Agreements and Marketing Orders § 900.26 Requesting USDA data for use at an amendatory hearing. Requests for preparation of USDA data to be used at a Federal milk marketing agreement or order...

  11. 7 CFR 900.40 - Written testimony and USDA data request requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Written testimony and USDA data request requirements... and Nut Marketing Agreements and Marketing Orders § 900.40 Written testimony and USDA data request... testimony, if prepared as an exhibit, and any other exhibits available to USDA before testimony is given...

  12. 7 CFR 900.40 - Written testimony and USDA data request requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Written testimony and USDA data request requirements... and Nut Marketing Agreements and Marketing Orders § 900.40 Written testimony and USDA data request... testimony, if prepared as an exhibit, and any other exhibits available to USDA before testimony is given...

  13. 7 CFR 900.26 - Requesting USDA data for use at an amendatory hearing.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Requesting USDA data for use at an amendatory hearing... Marketing Agreements and Marketing Orders § 900.26 Requesting USDA data for use at an amendatory hearing. Requests for preparation of USDA data to be used at a Federal milk marketing agreement or order...

  14. 7 CFR 170.5 - Is there a fee to participate in the USDA Farmers Market?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Is there a fee to participate in the USDA Farmers... (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.5 Is there a fee to participate in the USDA Farmers Market? No, there are no fees charged...

  15. USDA Northeast Climate Hub: delivering science-based knowledge and practical information

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Northeast Climate Hub is one of seven regional hubs created in February 2014 and is a partnership among USDA and other federal agencies, universities, Tribal governments, and state and private organizations within the northeast region from Maine to West Virginia. The USDA Northeast Climate ...

  16. Optimal hematologic variables for oxygen transport, including P50, hemoglobin cooperativity, hematocrit, acid-base status, and cardiac function.

    PubMed

    Winslow, R M

    1988-01-01

    The two important blood properties that affect O2 delivery are the O2 equilibrium curve (OEC) and blood viscosity with its subsequent effect on flow (cardiac output). To quantitate these properties blood OEC's were analyzed in terms of the Adair 4-step oxygenation model and the resulting parameters were used to construct a computer nomogram to reproduce the OEC at any combination of effectors that regulate P50 (pH, PCO2, and 2,3-DPG). In this way, the P50 could be changed systematically and the effects on overall O2 transport could be studied. Hematocrit-viscosity-cardiac output relationships were taken from the literature and validated using data from human subjects with various pathological states and high-altitude natives. A model was then developed, using the Bohr integration, to predict the O2 transport function of blood under a variety of conditions including exercise and hypoxia. The results indicate that the optimal hematocrit is about 43-45%, even in hypoxia. The optimal P50, however, depends on the availability of O2: a high P50 is not necessarily beneficial in hypoxia and high cardiac output states. This model and general approach should prove useful in the design of blood substitutes.

  17. USDA Section 9006 Program: Status and Energy Benefits of Grant Awards in FY 2003-2005

    SciTech Connect

    Walters, T.; Savage, S.; Brown, J.

    2006-08-01

    At the request of the U. S. Department of Agriculture (USDA) Rural Development, the National Renewable Energy Laboratory reviewed projects awarded in the Section 9006 Program: Renewable Energy Systems and Energy Efficiency Improvements Program. This report quantifies federal and private investment, outlines project status based on recent field updates, and calculates the effects on energy and emissions of energy efficiency and renewable energy projects awarded grants in FY 2003, FY 2004, and FY 2005. An overview of the program challenges and modifications in the first three years of operation is also included.

  18. Omega-3 fatty acid concentrate from Dunaliella salina possesses anti-inflammatory properties including blockade of NF-κB nuclear translocation.

    PubMed

    Chitranjali, T; Anoop Chandran, P; Muraleedhara Kurup, G

    2015-02-01

    The health benefits of omega-3 polyunsaturated fatty acids (ω-3 PUFA), mainly eicosapentaenoic acid (EPA 20:5) and docosahexaenoic acid (DHA, 22:6), have been long known. Although various studies have demonstrated the health benefits of ω-3 PUFA, the mechanisms of action of ω-3 PUFAs are still not completely understood. While the major commercial source is marine fish oil, in this study we suggest the marine micro algae, Dunaliella salina as an alternate source of omega-3 fatty acids. Treatment with this algal omega-3 fatty acid concentrate (Ds-ω-3 FA) resulted in significant down-regulation of LPS-induced production of TNF-α and IL-6 by peripheral blood mononuclear cells (PBMCs). The concentrate was also found to be a potent blocker of cyclooxygenase (COX-2) and matrix metalloproteinase (MMP-2 and MMP-9) expression. The present study reveals the anti-inflammatory properties of Ds-ω-3 FA concentrate including the inhibition of NF-κB translocation.

  19. USDA/ARS and dielectric properties research

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An overview of the research is presented, including RF dielectric heating for seed treatment, insect control, product conditioning, and moisture and quality sensing applications, equipment used, dielectric properties measurement techniques, broad- frequency- range data obtained, and research results...

  20. New force field parameters for metalloproteins I: Divalent copper ion centers including three histidine residues and an oxygen-ligated amino acid residue.

    PubMed

    Wise, Olivia; Coskuner, Orkid

    2014-06-30

    Transition metal ion complexation with proteins is ubiquitous across such diverse fields as neurodegenerative and cardiovascular diseases and cancer. In this study, the structures of divalent copper ion centers including three histidine and one oxygen-ligated amino acid residues and the relative binding affinities of the oxygen-ligated amino acid residues with these metal ion centers, which are debated in the literature, are presented. Furthermore, new force field parameters, which are currently lacking for the full-length metal-ligand moieties, are developed for metalloproteins that have these centers. These new force field parameters enable investigations of metalloproteins possessing these binding sites using molecular simulations. In addition, the impact of using the atom equivalence and inequivalence atomic partial charge calculation procedures on the simulated structures of these metallopeptides, including hydration properties, is described.

  1. USDA-ARS perspective on PAM

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Polyacrylamide (PAM) described herein is a synthetic organic polymer used globally by a number of important industries. It also has a number of valuable applications in irrigated agriculture, including its use in furrow irrigation to control erosion and sediment loss in runoff, manage infiltration,...

  2. NETL, USDA design coal-stabilized biomass gasification unit

    SciTech Connect

    2008-09-30

    Coal, poultry litter, contaminated corn, rice hulls, moldly hay, manure sludge - these are representative materials that could be tested as fuel feedstocks in a hybrid gasification/combustion concept studied in a recent US Department of Energy (DOE) design project. DOE's National Energy Technology Laboratory (NETL) and the US Department of Agriculture (USDA) collaborated to develop a design concept of a power system that incorporates Hybrid Biomass Gasification. This system would explore the use of a wide range of biomass and agricultural waste products as gasifier feedstocks. The plant, if built, would supply one-third of electrical and steam heating needs at the USDA's Beltsville (Maryland) Agricultural Research Center. 1 fig., 1 photo.

  3. Extension of a PBPK model for ethylene glycol and glycolic acid to include the competitive formation and clearance of metabolites associated with kidney toxicity in rats and humans

    SciTech Connect

    Corley, R.A.; Saghir, S.A.; Bartels, M.J.; Hansen, S.C.; Creim, J.; McMartin, K.E.; Snellings, W.M.

    2011-02-01

    A previously developed PBPK model for ethylene glycol and glycolic acid was extended to include glyoxylic acid, oxalic acid, and the precipitation of calcium oxalate that is associated with kidney toxicity in rats and humans. The development and evaluation of the PBPK model was based upon previously published pharmacokinetic studies coupled with measured blood and tissue partition coefficients and rates of in vitro metabolism of glyoxylic acid to oxalic acid, glycine and other metabolites using primary hepatocytes isolated from male Wistar rats and humans. Precipitation of oxalic acid with calcium in the kidneys was assumed to occur only at concentrations exceeding the thermodynamic solubility product for calcium oxalate. This solubility product can be affected by local concentrations of calcium and other ions that are expressed in the model using an ion activity product estimated from toxicity studies such that calcium oxalate precipitation would be minimal at dietary exposures below the NOAEL for kidney toxicity in the sensitive male Wistar rat. The resulting integrated PBPK predicts that bolus oral or dietary exposures to ethylene glycol would result in typically 1.4-1.6-fold higher peak oxalate levels and 1.6-2-fold higher AUC's for calcium oxalate in kidneys of humans as compared with comparably exposed male Wistar rats over a dose range of 1-1000 mg/kg. The converse (male Wistar rats predicted to have greater oxalate levels in the kidneys than humans) was found for inhalation exposures although no accumulation of calcium oxalate is predicted to occur until exposures are well in excess of the theoretical saturated vapor concentration of 200 mg/m{sup 3}. While the current model is capable of such cross-species, dose, and route-of-exposure comparisons, it also highlights several areas of potential research that will improve confidence in such predictions, especially at low doses relevant for most human exposures.

  4. USDA Finances Wind for Rural Economic Development (Poster)

    SciTech Connect

    Newcomb, C.; Walters, T.

    2005-05-01

    To foster rural economic development and growth, Congress passed the Renewable Energy Systems and Energy Efficiency Improvements Program as Section 9006 of the 2002 Farm Bill. This program provides financial assistance to farmers, ranchers, and rural small businesses to purchase renewable energy systems or make energy efficiency improvements. The Rural Business and Cooperative Services of the United States Department of Agriculture (USDA) administers this program. This conference poster provides an overview of Section 9006.

  5. Functional definition and design of a USDA system

    NASA Technical Reports Server (NTRS)

    Evans, S. M.; Dario, E. R.; Dickinson, G. L. (Principal Investigator)

    1979-01-01

    The fundamental definition and design of a U.S.D.A. system utilizing the LACIE technology avaliable as of June 1976, is discussed. The organization and methods described are focused on LACIE technology in terms of its transfer for use applications. The simulation of a feasible system design provided timely answers to system design questions, such as the ability of a minicomputer to handle the proposed geometrical correction of MSS data.

  6. Assessing human health risk in the USDA forest service

    SciTech Connect

    Hamel, D.R.

    1990-12-31

    This paper identifies the kinds of risk assessments being done by or for the US Department of Agriculture (USDA) Forest Service. Summaries of data sources currently in use and the pesticide risk assessments completed by the agency or its contractors are discussed. An overview is provided of the agency`s standard operating procedures for the conduct of toxicological, ecological, environmental fate, and human health risk assessments.

  7. 7 CFR 15f.5 - How do I request that USDA consider my complaint under these procedures?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false How do I request that USDA consider my complaint under... UNDER SECTION 741 I Filed a Complaint With USDA Prior to July 1, 1997, How Do I Request That USDA Consider My Complaint Using These Procedures? § 15f.5 How do I request that USDA consider my...

  8. 2 CFR 417.221 - How would the exclusions from coverage for the USDA's foreign assistance programs apply?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... the USDA's foreign assistance programs apply? 417.221 Section 417.221 Grants and Agreements Federal... SUSPENSION Covered Transactions § 417.221 How would the exclusions from coverage for the USDA's foreign... into between USDA and a program participant, such as a U.S. private voluntary organization. USDA...

  9. 7 CFR 15f.2 - Who may use these procedures for processing their discrimination complaint with USDA?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... discrimination complaint with USDA? 15f.2 Section 15f.2 Agriculture Office of the Secretary of Agriculture... USDA? A person may use these procedures if he or she filed a nonemployment related discrimination complaint with USDA prior to July 1, 1997, that alleged discrimination by USDA at any time during the...

  10. 2 CFR 417.221 - How would the exclusions from coverage for the USDA's foreign assistance programs apply?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... the USDA's foreign assistance programs apply? 417.221 Section 417.221 Grants and Agreements Federal... SUSPENSION Covered Transactions § 417.221 How would the exclusions from coverage for the USDA's foreign... into between USDA and a program participant, such as a U.S. private voluntary organization. USDA...

  11. 7 CFR 15f.2 - Who may use these procedures for processing their discrimination complaint with USDA?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... discrimination complaint with USDA? 15f.2 Section 15f.2 Agriculture Office of the Secretary of Agriculture... USDA? A person may use these procedures if he or she filed a nonemployment related discrimination complaint with USDA prior to July 1, 1997, that alleged discrimination by USDA at any time during the...

  12. 7 CFR 15f.5 - How do I request that USDA consider my complaint under these procedures?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false How do I request that USDA consider my complaint under... UNDER SECTION 741 I Filed a Complaint With USDA Prior to July 1, 1997, How Do I Request That USDA Consider My Complaint Using These Procedures? § 15f.5 How do I request that USDA consider my...

  13. 7 CFR 170.12 - What are the selection criteria for participation in the USDA Farmers Market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... the USDA Farmers Market? 170.12 Section 170.12 Agriculture Regulations of the Department of... OF 1946 USDA FARMERS MARKET § 170.12 What are the selection criteria for participation in the USDA.... Participants should commit to supporting the USDA food gleaning/food recovery initiative. This...

  14. 7 CFR 15f.2 - Who may use these procedures for processing their discrimination complaint with USDA?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... discrimination complaint with USDA? 15f.2 Section 15f.2 Agriculture Office of the Secretary of Agriculture... USDA? A person may use these procedures if he or she filed a nonemployment related discrimination complaint with USDA prior to July 1, 1997, that alleged discrimination by USDA at any time during the...

  15. 7 CFR 170.11 - How are farmers and vendors selected for participation in the USDA Farmers Market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... in the USDA Farmers Market? 170.11 Section 170.11 Agriculture Regulations of the Department of... OF 1946 USDA FARMERS MARKET § 170.11 How are farmers and vendors selected for participation in the USDA Farmers Market? USDA reviews all applications and selects participants based primarily on the...

  16. 7 CFR 15f.5 - How do I request that USDA consider my complaint under these procedures?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false How do I request that USDA consider my complaint under... UNDER SECTION 741 I Filed a Complaint With USDA Prior to July 1, 1997, How Do I Request That USDA Consider My Complaint Using These Procedures? § 15f.5 How do I request that USDA consider my...

  17. 2 CFR 417.221 - How would the exclusions from coverage for the USDA's foreign assistance programs apply?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... the USDA's foreign assistance programs apply? 417.221 Section 417.221 Grants and Agreements Federal... SUSPENSION Covered Transactions § 417.221 How would the exclusions from coverage for the USDA's foreign... into between USDA and a program participant, such as a U.S. private voluntary organization. USDA...

  18. 2 CFR 417.221 - How would the exclusions from coverage for the USDA's foreign assistance programs apply?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... the USDA's foreign assistance programs apply? 417.221 Section 417.221 Grants and Agreements Federal... SUSPENSION Covered Transactions § 417.221 How would the exclusions from coverage for the USDA's foreign... into between USDA and a program participant, such as a U.S. private voluntary organization. USDA...

  19. 7 CFR 15f.2 - Who may use these procedures for processing their discrimination complaint with USDA?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... discrimination complaint with USDA? 15f.2 Section 15f.2 Agriculture Office of the Secretary of Agriculture... USDA? A person may use these procedures if he or she filed a nonemployment related discrimination complaint with USDA prior to July 1, 1997, that alleged discrimination by USDA at any time during the...

  20. 7 CFR 170.11 - How are farmers and vendors selected for participation in the USDA Farmers Market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... in the USDA Farmers Market? 170.11 Section 170.11 Agriculture Regulations of the Department of... OF 1946 USDA FARMERS MARKET § 170.11 How are farmers and vendors selected for participation in the USDA Farmers Market? USDA reviews all applications and selects participants based primarily on the...

  1. 7 CFR 15f.2 - Who may use these procedures for processing their discrimination complaint with USDA?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... discrimination complaint with USDA? 15f.2 Section 15f.2 Agriculture Office of the Secretary of Agriculture... USDA? A person may use these procedures if he or she filed a nonemployment related discrimination complaint with USDA prior to July 1, 1997, that alleged discrimination by USDA at any time during the...

  2. 7 CFR 170.12 - What are the selection criteria for participation in the USDA Farmers Market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... the USDA Farmers Market? 170.12 Section 170.12 Agriculture Regulations of the Department of... OF 1946 USDA FARMERS MARKET § 170.12 What are the selection criteria for participation in the USDA.... Participants should commit to supporting the USDA food gleaning/food recovery initiative. This...

  3. 7 CFR 15f.5 - How do I request that USDA consider my complaint under these procedures?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false How do I request that USDA consider my complaint under... UNDER SECTION 741 I Filed a Complaint With USDA Prior to July 1, 1997, How Do I Request That USDA Consider My Complaint Using These Procedures? § 15f.5 How do I request that USDA consider my...

  4. 7 CFR 15f.5 - How do I request that USDA consider my complaint under these procedures?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false How do I request that USDA consider my complaint under... UNDER SECTION 741 I Filed a Complaint With USDA Prior to July 1, 1997, How Do I Request That USDA Consider My Complaint Using These Procedures? § 15f.5 How do I request that USDA consider my...

  5. Impacts of climate change on Southwestern working lands and water resources: a report from the inaugural year of the USDA Southwest climate hub

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In February 2014 the USDA established seven regional climate hubs to assist farmers, ranchers and foresters in adapting to the effects of climate change. The SW region encompasses six states (including Hawaii) and provides highly diverse agricultural crops including cotton, stone fruit and grapes. ...

  6. Targeted mutation of Δ12 and Δ15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil.

    PubMed

    Bielecka, Monika; Kaminski, Filip; Adams, Ian; Poulson, Helen; Sloan, Raymond; Li, Yi; Larson, Tony R; Winzer, Thilo; Graham, Ian A

    2014-06-01

    We used expressed sequence tag library and whole genome sequence mining to identify a suite of putative desaturase genes representing the four main activities required for production of polyunsaturated fatty acids in hemp seed oil. Phylogenetic-based classification and developing seed transcriptome analysis informed selection for further analysis of one of seven Δ12 desaturases and one of three Δ15 desaturases that we designate CSFAD2A and CSFAD3A, respectively. Heterologous expression of corresponding cDNAs in Saccharomyces cerevisiae showed CSFAD2A to have Δx+3 activity, while CSFAD3A activity was exclusively at the Δ15 position. TILLING of an ethyl methane sulphonate mutagenized population identified multiple alleles including non-sense mutations in both genes and fatty acid composition of seed oil confirmed these to be the major Δ12 and Δ15 desaturases in developing hemp seed. Following four backcrosses and sibling crosses to achieve homozygosity, csfad2a-1 was grown in the field and found to produce a 70 molar per cent high oleic acid (18:1(Δ9) ) oil at yields similar to wild type. Cold-pressed high oleic oil produced fewer volatiles and had a sevenfold increase in shelf life compared to wild type. Two low abundance octadecadienoic acids, 18:2(Δ6,9) and 18:2(Δ9,15), were identified in the high oleic oil, and their presence suggests remaining endogenous desaturase activities utilize the increased levels of oleic acid as substrate. Consistent with this, CSFAD3A produces 18:2(Δ9,15) from endogenous 18:1(Δ9) when expressed in S. cerevisiae. This work lays the foundation for the development of additional novel oil varieties in this multipurpose low input crop.

  7. Irradiation of ready-to-eat foods at USDA'S Eastern Regional Reasearch Center-2003 update

    NASA Astrophysics Data System (ADS)

    Sommers, Christopher; Fan, Xuetong; Niemira, Brendan; Rajkowski, Kathleen

    2004-09-01

    Ionizing radiation is a safe and effective method for eliminating bacterial pathogens from food products and disinfestation of fruits and vegetables. Since 1980 research has been conducted at USDA's Eastern Regional Research Center pertaining to the elimination of food-borne pathogens from meat, poultry, fruit and vegetable products. Recent work has focused on elimination of pathogens such as Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes from ready-to-eat (RTE) food products including hot dogs, bologna, lettuce, cilantro, sprouts and seeds, and frozen vegetables. The ionizing radiation dose required to eliminate those pathogens from RTE foods has been found to be commodity, formulation and temperature dependent. The need to eliminate bacterial pathogens from RTE food products must always be balanced with the maintenance of product quality. In addition to determining the effective ionizing radiation doses required for pathogen elimination the effects of irradiation on product chemistry, nutritional value and organoleptic quality have also been determined. A review of the studies conducted at USDA's Eastern Regional Research Center in 2002 and 2003 is presented in this article.

  8. Total lipids of Sarda sheep meat that include the fatty acid and alkenyl composition and the CLA and trans-18:1 isomers.

    PubMed

    Santercole, Viviana; Mazzette, Rina; De Santis, Enrico P L; Banni, Sebastiano; Goonewardene, Laki; Kramer, John K G

    2007-04-01

    The total lipids of the longissimus dorsi muscle were analyzed from commercial adult Sarda sheep in Sardina taken from local abattoirs, and in the subsequent year from three local farms in the Sassari region that provided some information on the amount and type of supplements fed to the pasture-fed sheep. The complete lipid analysis of sheep meat included the fatty acids from O-acyl and N-acyl lipids, including the trans- and conjugated linoleic acid (CLA) isomers and the alk-1-enyl ethers from the plasmalogenic lipids. This analysis required the use of a combination of acid- and base-catalyzed methylation procedures, the former to quantitate the O-acyl, N-acyl and alkenyl ethers, and the latter to determine the content of CLA isomers and their metabolites. A combination of gas chromatographic and silver-ion separation techniques was necessary to quantitate all of the meat lipid constituents, which included a prior separation of the trans-octadecenoic acids (18:1) and a separation of fatty acid methyl esters and the dimethylacetals (DMAs) from the acyl and alk-1-enyl ethers, respectively. The alk-1-enyl moieties of the DMAs were analyzed as their stable cyclic acetals. In general, about half of the meat lipids were triacylglycerols, even though excess fat was trimmed from the meat. The higher fat content in the meat appears to be related to the older age of these animals. The variation in the trans-18:1 and CLA isomer profiles of the Sarda sheep obtained from the abattoirs was much greater than in the profiles from the sheep from the three selected farms. Higher levels of 10t-18:1, 7t9c-18:2, 9t11c-18:2 and 10t12c-18:2 were observed in the commercial sheep meat, which reflected the poorer quality diets of these sheep compared to those from the three farms, which consistently showed higher levels of 11t-18:1, 9c11t-18:2 and 11t13c-18:2. In the second study, sheep were provided with supplements during the spring and summer grazing season, which contributed to higher

  9. Thermodynamic models of aqueous solutions containing inorganic electrolytes and dicarboxylic acids at 298.15 K. 2. Systems including dissociation equilibria.

    PubMed

    Clegg, Simon L; Seinfeld, John H

    2006-05-01

    Atmospheric aerosols contain a significant fraction of water-soluble organic compounds, including dicarboxylic acids. Pitzer activity coefficient models are developed, using a wide range of data at 298.15 K, for the following systems containing succinic acid (H(2)Succ) and/or succinate salts: [H(+), Li(+), Na(+), K(+), Rb(+), Cs(+)]Cl(-)-H(2)Succ-H(2)O, HNO(3)-H(2)Succ-H(2)O, H(+)-NH(4)(+)-HSucc(-)-Succ(2-)-NH(3)-H(2)Succ-H(2)O, NH(4)Cl-(NH(4))(2)Succ-H(2)O, H(+)-Na(+)-HSucc(-)-Succ(2-)-Cl(-)-H(2)Succ-H(2)O, NH(4)NO(3)-H(2)Succ-H(2)O, and H(2)SO(4)-H(2)Succ-H(2)O. The above compositions are given in terms of ions in the cases where acid dissociation was considered. Pitzer models were also developed for the following systems containing malonic acid (H(2)Malo): H(+)-Na(+)-HMalo(-)-Malo(2-)-Cl(-)-H(2)Malo-H(2)O, and H(2)Malo-H(2)SO(4)-H(2)O. The models are used to evaluate the extended Zdanovskii-Stokes-Robinson (ZSR) model proposed by Clegg and Seinfeld (J. Phys. Chem. A 2004, 108, 1008-1017) for calculating water and solute activities in solutions in which dissociation equilibria occur. The ZSR model yields satisfactory results only for systems that contain moderate to high concentrations of (nondissociating) supporting electrolyte. A practical modeling scheme is proposed for aqueous atmospheric aerosols containing both electrolytes and dissociating (organic) nonelectrolytes.

  10. Isolation and partial characterization of the extracellular polysaccharides and lipopolysaccharides from fast-growing Rhizobium japonicum USDA 205 and its Nod- mutant, HC205, which lacks the symbiotic plasmid.

    PubMed Central

    Carlson, R W; Yadav, M

    1985-01-01

    The extracellular polysaccharides and lipopolysaccharides (LPSs) from two fast-growing Rhizobium japonicum strains, USDA 205 and HC205, were isolated and partially characterized. Strain HC205 is a Nod- mutant of USDA 205 which lacks the symbiotic plasmid. The extracellular polysaccharides from both strains are very similar in composition, having galactose, glucose, glucuronic acid, and acyl groups. The extracellular polysaccharides do not contain detectable levels of pyruvate. Methylation analysis shows that the extracellular polysaccharides from both strains have the same glycosyl linkages. The LPSs were purified by a modified phenol-water extraction procedure and gel filtration chromatography. The LPSs from USDA 205 and HC205 elute as broad peaks from the gel filtration column and contain 2-keto-3-deoxyoctonic acid as one of the major sugar components. Each broad 2-keto-3-deoxyoctonic acid-containing peak has a distinct shoulder on its leading edge. The shoulder and the remainder of the broad peak are separated and labeled LPSI and LPSII, respectively. Glucose (and 2-keto-3-deoxyoctonic acid) is a major sugar in the LPSI fractions. Both the LPSII fractions contain 2-keto-3-deoxyoctonic acid as the major sugar (about 20% of the mass). There are a number of quantitative differences in these LPS fractions between strain USDA 205 and HC205. Polyacrylamide gel electrophoresis shows that the LPSs are heterogeneous molecules but less heterogeneous than the LPSs from Salmonella minnesota or Rhizobium leguminosarum. The LPSI fractions from both USDA 205 and HC205 show a single lower-molecular-weight band and a higher-molecular-weight banding region which contains several bands. No bands are observed for the LPSII fractions from either USDA 205 or HC205. Images PMID:4091554

  11. A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication.

    PubMed

    Koonin, E V

    1993-06-11

    A new superfamily of (putative) DNA-dependent ATPases is described that includes the ATPase domains of prokaryotic NtrC-related transcription regulators, MCM proteins involved in the initiation of eukaryotic DNA replication, and a group of uncharacterized bacterial and chloroplast proteins. MCM proteins are shown to contain a modified form of the ATP-binding motif and are predicted to mediate ATP-dependent opening of double-stranded DNA in the replication origins. In a second line of investigation, it is demonstrated that the products of unidentified open reading frames from Marchantia mitochondria and from yeast, and a domain of a baculovirus protein involved in viral DNA replication are related to the superfamily III of DNA and RNA helicases that previously has been known to include only proteins of small viruses. Comparison of the multiple alignments showed that the proteins of the NtrC superfamily and the helicases of superfamily III share three related sequence motifs tightly packed in the ATPase domain that consists of 100-150 amino acid residues. A similar array of conserved motifs is found in the family of DnaA-related ATPases. It is hypothesized that the three large groups of nucleic acid-dependent ATPases have similar structure of the core ATPase domain and have evolved from a common ancestor.

  12. NASA's Agricultural Program: A USDA/Grower Partnership

    NASA Technical Reports Server (NTRS)

    McKellip, Rodney; Thomas, Michael

    2002-01-01

    Ag20/20 is a partnership between USDA, NASA, and four national commodity associations. It is driven by the information needs of U.S. farmers. Ag20/20 is focused on utilization of earth science and remote sensing for decision-making and oriented toward economically viable operational solutions. Its purpose is to accelerate the use of remote sensing and other geospatial technologies on the farm to: 1) Increase the production efficiency of the American farmer; 2) Reduce crop production risks; 3) Improve environmental stewardship tools for agricultural production.

  13. Multi-Biomarkers for Early Detection of Type 2 Diabetes, Including 10- and 12-(Z,E)-Hydroxyoctadecadienoic Acids, Insulin, Leptin, and Adiponectin

    PubMed Central

    Umeno, Aya; Yoshino, Kohzoh; Hashimoto, Yoshiko; Shichiri, Mototada; Kataoka, Masatoshi; Yoshida, Yasukazu

    2015-01-01

    We have previously found that fasting plasma levels of totally assessed 10- and 12-(Z,E)-hydroxyoctadecadienoic acid (HODE) correlated well with levels of glycated hemoglobin (HbA1c) and glucose during oral glucose tolerance tests (OGTT); these levels were determined via liquid chromatography—mass spectrometry after reduction and saponification. However, 10- and 12-(Z,E)-HODE alone cannot perfectly detect early impaired glucose tolerance (IGT) and/or insulin resistance, which ultimately lead to diabetes. In this study, we randomly recruited healthy volunteers (n = 57) who had no known history of any diseases, and who were evaluated using the OGTT, the HODE biomarkers, and several additional proposed biomarkers, including retinol binding protein 4 (RBP4), adiponectin, leptin, insulin, glycoalbumin, and high sensitivity-C-reactive protein. The OGTT revealed that our volunteers included normal individuals (n = 44; Group N), “high-normal” individuals (fasting plasma glucose 100–109 mg/dL) with IGT (n = 11; Group HN+IGT), and diabetic individuals (n = 2; Group D). We then used these groups to evaluate the potential biomarkers for the early detection of type 2 diabetes. Plasma levels of RBP4 and glycoalbumin were higher in Group HN+IGT, compared to those in Group N, and fasting levels of 10- and 12-(Z,E)-HODE/linoleic acids were significantly correlated with levels of RBP4 (p = 0.003, r = 0.380) and glycoalbumin (p = 0.006, r = 0.316). Furthermore, we developed a stepwise multiple linear regression models to predict the individuals’ insulin resistance index (the Matsuda Index 3). Fasting plasma levels of 10- and 12-(Z,E)-HODE/linoleic acids, glucose, insulin, and leptin/adiponectin were selected as the explanatory variables for the models. The risks of type 2 diabetes, early IGT, and insulin resistance were perfectly predicted by comparing fasting glucose levels to the estimated Matsuda Index 3 (fasting levels of 10- and 12-(Z,E)-HODE/linoleic acids, insulin

  14. Enteric Bacterial Metabolites Propionic and Butyric Acid Modulate Gene Expression, Including CREB-Dependent Catecholaminergic Neurotransmission, in PC12 Cells - Possible Relevance to Autism Spectrum Disorders

    PubMed Central

    Nankova, Bistra B.; Agarwal, Raj; MacFabe, Derrick F.; La Gamma, Edmund F.

    2014-01-01

    Alterations in gut microbiome composition have an emerging role in health and disease including brain function and behavior. Short chain fatty acids (SCFA) like propionic (PPA), and butyric acid (BA), which are present in diet and are fermentation products of many gastrointestinal bacteria, are showing increasing importance in host health, but also may be environmental contributors in neurodevelopmental disorders including autism spectrum disorders (ASD). Further to this we have shown SCFA administration to rodents over a variety of routes (intracerebroventricular, subcutaneous, intraperitoneal) or developmental time periods can elicit behavioral, electrophysiological, neuropathological and biochemical effects consistent with findings in ASD patients. SCFA are capable of altering host gene expression, partly due to their histone deacetylase inhibitor activity. We have previously shown BA can regulate tyrosine hydroxylase (TH) mRNA levels in a PC12 cell model. Since monoamine concentration is known to be elevated in the brain and blood of ASD patients and in many ASD animal models, we hypothesized that SCFA may directly influence brain monoaminergic pathways. When PC12 cells were transiently transfected with plasmids having a luciferase reporter gene under the control of the TH promoter, PPA was found to induce reporter gene activity over a wide concentration range. CREB transcription factor(s) was necessary for the transcriptional activation of TH gene by PPA. At lower concentrations PPA also caused accumulation of TH mRNA and protein, indicative of increased cell capacity to produce catecholamines. PPA and BA induced broad alterations in gene expression including neurotransmitter systems, neuronal cell adhesion molecules, inflammation, oxidative stress, lipid metabolism and mitochondrial function, all of which have been implicated in ASD. In conclusion, our data are consistent with a molecular mechanism through which gut related environmental signals such as

  15. Amino acids 16-275 of minute virus of mice NS1 include a domain that specifically binds (ACCA)2-3-containing DNA.

    PubMed

    Mouw, M; Pintel, D J

    1998-11-10

    GST-NS1 purified from Escherichia coli and insect cells binds double-strand DNA in an (ACCA)2-3-dependent fashion under similar ionic conditions, independent of the presence of anti-NS1 antisera or exogenously supplied ATP and interacts with single-strand DNA and RNA in a sequence-independent manner. An amino-terminal domain (amino acids 1-275) of NS1 [GST-NS1(1-275)], representing 41% of the full-length NS1 molecule, includes a domain that binds double-strand DNA in a sequence-specific manner at levels comparable to full-length GST-NS1, as well as single-strand DNA and RNA in a sequence-independent manner. The deletion of 15 additional amino-terminal amino acids yielded a molecule [GST-NS1(1-275)] that maintained (ACCA)2-3-specific double-strand DNA binding; however, this molecule was more sensitive to increasing ionic conditions than full-length GST-NS1 and GST-NS1(1-275) and could not be demonstrated to bind single-strand nucleic acids. A quantitative filter binding assay showed that E. coli- and baculovirus-expressed GST-NS1 and E. coli GST-NS1(1-275) specifically bound double-strand DNA with similar equilibrium kinetics [as measured by their apparent equilibrium DNA binding constants (KD)], whereas GST-NS1(16-275) bound 4- to 8-fold less well.

  16. An L-glucitol oxidizing dehydrogenase from Bradyrhizobium japonicum USDA 110 for production of D-sorbose with enzymatic or electrochemical cofactor regeneration.

    PubMed

    Gauer, Sabrina; Wang, Zhijie; Otten, Harm; Etienne, Mathieu; Bjerrum, Morten Jannik; Lo Leggio, Leila; Walcarius, Alain; Giffhorn, Friedrich; Kohring, Gert-Wieland

    2014-04-01

    A gene in Bradyrhizobium japonicum USDA 110, annotated as a ribitol dehydrogenase (RDH), had 87 % sequence identity (97 % positives) to the N-terminal 31 amino acids of an L-glucitol dehydrogenase from Stenotrophomonas maltophilia DSMZ 14322. The 729-bp long RDH gene coded for a protein consisting of 242 amino acids with a molecular mass of 26.1 kDa. The heterologously expressed protein not only exhibited the main enantio selective activity with D-glucitol oxidation to D-fructose but also converted L-glucitol to D-sorbose with enzymatic cofactor regeneration and a yield of 90 %. The temperature stability and the apparent K m value for L-glucitol oxidation let the enzyme appear as a promising subject for further improvement by enzyme evolution. We propose to rename the enzyme from the annotated RDH gene (locus tag bll6662) from B. japonicum USDA as a D-sorbitol dehydrogenase (EC 1.1.1.14).

  17. An update on potato crisps contents of moisture, fat, salt and fatty acids (including trans-fatty acids) with special emphasis on new oils/fats used for frying.

    PubMed

    Gonçalves Albuquerque, Tânia; Sanches-Silva, Ana; Santos, Lèlita; Costa, Helena S

    2012-09-01

    Eighteen brands of potato crisps, frequently consumed, were analyzed to establish their nutritional value in relation to salt, fat and fatty acid (FA) composition. The purpose of the present study was to determine moisture, total fat, salt contents and FA profiles (including trans-FAs), and to identify the oil/fat used for frying of the 18 brands of potato crisps. Our results show that salt content ranged from 0.127 to 2.77 g/100 g and total fat content of potato crisps varied between 20.0 and 42.8 g/100 g. With respect to FAs analysis, palmitic acid (C16:0), oleic acid (C18:1) and linoleic acid (C18:2) were the major FAs found in the analyzed potato crisps. It is clear from our work that nowadays most potato crisps are currently produced using oils with high contents in unsaturated FAs, which can be considered as healthier from a nutritional point of view. Nevertheless, some brands of potato crisps still use palm oil or a blend of palm oil and other fats/oils, which are very rich in saturated FAs.

  18. Complete genome sequence of the broad-host-range strain Sinorhizobium fredii USDA257

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Here we announce the complete genome sequence of the symbiotic and nitrogen fixing bacterium Sinorhizobium fredii USDA257. The genome shares a high degree of similarity with the closely related broad-host-range strains S. fredii NGR234 and HH103. Most striking, the USDA257 genome encodes for a wealt...

  19. Development of novel repellents using structure-activity modeling of compounds in the USDA archival database

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The United States Department of Agriculture (USDA) has developed repellents and insecticides for the U.S. military since 1942. Repellency and toxicity data for over 30,000 compounds are contained within the USDA archive. Repellency data from subsets of similarly structured compounds were used to dev...

  20. 7 CFR 170.4 - Who may participate in the USDA Farmers Market?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Who may participate in the USDA Farmers Market? 170.4 Section 170.4 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING... MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.4 Who...

  1. 7 CFR 170.4 - Who may participate in the USDA Farmers Market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Who may participate in the USDA Farmers Market? 170.4 Section 170.4 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING... MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.4 Who...

  2. 7 CFR 170.4 - Who may participate in the USDA Farmers Market?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Who may participate in the USDA Farmers Market? 170.4 Section 170.4 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING... MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.4 Who...

  3. 7 CFR 170.4 - Who may participate in the USDA Farmers Market?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Who may participate in the USDA Farmers Market? 170.4 Section 170.4 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING... MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.4 Who...

  4. USDA's Systematic Entomology Laboratory (SEL): Global leadership and innovation in insect systematics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A longstanding agreement between the USDA and the Smithsonian Institution created the National Insect Collection, one of the premiere entomological resources on the planet. Since its inception, USDA scientists associated with the National Insect Collection have been at the forefront of documenting i...

  5. 7 CFR 1945.18 - United States Department of Agriculture (USDA) Food and Agriculture Council (FAC).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... the SFACs. (b) Local Food and Agriculture Council (LFAC). These councils are composed of... 7 Agriculture 13 2012-01-01 2012-01-01 false United States Department of Agriculture (USDA) Food...) EMERGENCY Disaster Assistance-General § 1945.18 United States Department of Agriculture (USDA) Food...

  6. 7 CFR 1945.18 - United States Department of Agriculture (USDA) Food and Agriculture Council (FAC).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the SFACs. (b) Local Food and Agriculture Council (LFAC). These councils are composed of... 7 Agriculture 13 2010-01-01 2009-01-01 true United States Department of Agriculture (USDA) Food...) EMERGENCY Disaster Assistance-General § 1945.18 United States Department of Agriculture (USDA) Food...

  7. 7 CFR 1945.18 - United States Department of Agriculture (USDA) Food and Agriculture Council (FAC).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... the SFACs. (b) Local Food and Agriculture Council (LFAC). These councils are composed of... 7 Agriculture 13 2011-01-01 2009-01-01 true United States Department of Agriculture (USDA) Food...) EMERGENCY Disaster Assistance-General § 1945.18 United States Department of Agriculture (USDA) Food...

  8. USDA nutrient data set for beef cuts, release 2.0

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Nutrient Data Laboratory (NDL), in collaboration with beef industry and various universities, has conducted three different research studies designed to update or expand the data on beef cuts in the USDA National Nutrient Database for Standard Reference (SR). This research has been necessar...

  9. 7 CFR Appendix 1 to Part 799 - Organization Chart FSA-USDA

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 7 2013-01-01 2013-01-01 false Organization Chart FSA-USDA 1 Appendix 1 to Part 799 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF...-USDA EC19SE91.000...

  10. 7 CFR 15f.25 - Will USDA pay my attorneys fees if I win?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 CFR part 1, subpart J. The ALJ must follow those rules, and not these Section 741 Complaint... 7 Agriculture 1 2013-01-01 2013-01-01 false Will USDA pay my attorneys fees if I win? 15f.25... Request a Hearing, What Will Happen? How Will the Hearing Be Conducted? § 15f.25 Will USDA pay...

  11. 7 CFR Appendix 1 to Part 799 - Organization Chart FSA-USDA

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Organization Chart FSA-USDA 1 Appendix 1 to Part 799 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF...-USDA EC19SE91.000...

  12. 7 CFR 15f.25 - Will USDA pay my attorneys fees if I win?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 CFR part 1, subpart J. The ALJ must follow those rules, and not these Section 741 Complaint... 7 Agriculture 1 2014-01-01 2014-01-01 false Will USDA pay my attorneys fees if I win? 15f.25... Request a Hearing, What Will Happen? How Will the Hearing Be Conducted? § 15f.25 Will USDA pay...

  13. 7 CFR Appendix 1 to Part 799 - Organization Chart FSA-USDA

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false Organization Chart FSA-USDA 1 Appendix 1 to Part 799 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF...-USDA EC19SE91.000...

  14. 7 CFR 4290.509 - Employment of USDA or SBA officials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Employment of USDA or SBA officials. 4290.509 Section... (âRBICâ) PROGRAM Managing the Operations of a RBIC General Requirements § 4290.509 Employment of USDA... professional services, any person who: (1) Served as an officer, attorney, agent, or employee of SBA or...

  15. 2 CFR 417.870 - When do I know if the USDA debarring official debars me?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... transactions and contracts that are subject to the Federal Acquisition Regulation (48 CFR chapter 1... 2 Grants and Agreements 1 2012-01-01 2012-01-01 false When do I know if the USDA debarring... know if the USDA debarring official debars me? (a) The debarring official must make a written...

  16. 7 CFR 4290.509 - Employment of USDA or SBA officials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Employment of USDA or SBA officials. 4290.509 Section... (âRBICâ) PROGRAM Managing the Operations of a RBIC General Requirements § 4290.509 Employment of USDA... professional services, any person who: (1) Served as an officer, attorney, agent, or employee of SBA or...

  17. 2 CFR 417.870 - When do I know if the USDA debarring official debars me?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... transactions and contracts that are subject to the Federal Acquisition Regulation (48 CFR chapter 1... 2 Grants and Agreements 1 2011-01-01 2011-01-01 false When do I know if the USDA debarring... know if the USDA debarring official debars me? (a) The debarring official must make a written...

  18. 2 CFR 417.870 - When do I know if the USDA debarring official debars me?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... transactions and contracts that are subject to the Federal Acquisition Regulation (48 CFR chapter 1... 2 Grants and Agreements 1 2013-01-01 2013-01-01 false When do I know if the USDA debarring... know if the USDA debarring official debars me? (a) The debarring official must make a written...

  19. 2 CFR 417.870 - When do I know if the USDA debarring official debars me?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... transactions and contracts that are subject to the Federal Acquisition Regulation (48 CFR chapter 1... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false When do I know if the USDA debarring... know if the USDA debarring official debars me? (a) The debarring official must make a written...

  20. 7 CFR 15f.25 - Will USDA pay my attorneys fees if I win?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 CFR part 1, subpart J. The ALJ must follow those rules, and not these Section 741 Complaint... 7 Agriculture 1 2012-01-01 2012-01-01 false Will USDA pay my attorneys fees if I win? 15f.25... Request a Hearing, What Will Happen? How Will the Hearing Be Conducted? § 15f.25 Will USDA pay...

  1. 7 CFR 15f.25 - Will USDA pay my attorneys fees if I win?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 CFR part 1, subpart J. The ALJ must follow those rules, and not these Section 741 Complaint... 7 Agriculture 1 2011-01-01 2011-01-01 false Will USDA pay my attorneys fees if I win? 15f.25... Request a Hearing, What Will Happen? How Will the Hearing Be Conducted? § 15f.25 Will USDA pay...

  2. 7 CFR Appendix 1 to Part 799 - Organization Chart FSA-USDA

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Organization Chart FSA-USDA 1 Appendix 1 to Part 799 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF...-USDA EC19SE91.000...

  3. 7 CFR 15f.25 - Will USDA pay my attorneys fees if I win?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 CFR part 1, subpart J. The ALJ must follow those rules, and not these Section 741 Complaint... 7 Agriculture 1 2010-01-01 2010-01-01 false Will USDA pay my attorneys fees if I win? 15f.25... Request a Hearing, What Will Happen? How Will the Hearing Be Conducted? § 15f.25 Will USDA pay...

  4. USDA-ARS extension activities in medical, veterinary and urban entomology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Within the USDA Agricultural Research Service (USDA-ARS), National Program 104 conducts research on veterinary, medical, and urban entomology. The goal of this program is to develop more effective methods of preventing or suppressing insects, ticks, and mites that affect animal and human well-being....

  5. Conservation of the USDA-ARS National Plant Germplasm System Apple Collection using dormant bud cryopreservation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-ARS National Plant Germplasm System maintains a grafted collection of apple accessions representing 49 taxa in Geneva, NY. Since 1993, dormant buds of many of these accessions have been routinely cryopreserved at the USDA-ARS National Center for Genetic Resources Preservation (NCGRP) in For...

  6. Beet curly top resistance of USDA-ARS National Plant Germplasm System plant introductions, 2009

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Thirty wild beet (Beta vulgaris subsp. maritima (L.) Arcang) accessions from the Beta collection of the USDA-ARS National Plant Germplasm System were screened for resistance to Beet severe curly top virus (BSCTV) in 2009. The curly top evaluation was conducted at the USDA-ARS North Farm in Kimberly...

  7. Harvesting Greatness: With Support from the USDA, Rural Colleges Are Expanding Campuses and Helping Communities

    ERIC Educational Resources Information Center

    Boerner, Heather

    2015-01-01

    Partnerships between the USDA and rural colleges are one approach that allows individual students and regional economies to fulfill their potential. The U.S. Department of Agriculture (USDA) has made a decision to be involved with rural community colleges, which has really made a difference on what is happening on campus. Through a dizzying array…

  8. Particle-Size Distribution Models for the Conversion of Chinese Data to FAO/USDA System

    PubMed Central

    Dai, YongJiu; García-Gutiérrez, Carlos; Yuan, Hua

    2014-01-01

    We investigated eleven particle-size distribution (PSD) models to determine the appropriate models for describing the PSDs of 16349 Chinese soil samples. These data are based on three soil texture classification schemes, including one ISSS (International Society of Soil Science) scheme with four data points and two Katschinski's schemes with five and six data points, respectively. The adjusted coefficient of determination r 2, Akaike's information criterion (AIC), and geometric mean error ratio (GMER) were used to evaluate the model performance. The soil data were converted to the USDA (United States Department of Agriculture) standard using PSD models and the fractal concept. The performance of PSD models was affected by soil texture and classification of fraction schemes. The performance of PSD models also varied with clay content of soils. The Anderson, Fredlund, modified logistic growth, Skaggs, and Weilbull models were the best. PMID:25121108

  9. Nutritional assessment of genetically modified rapeseed synthesizing high amounts of mid-chain fatty acids including production responses of growing-finishing pigs.

    PubMed

    Böhme, Hartwig; Rudloff, Eike; Schöne, Friedrich; Schumann, Wolfgang; Hüther, Liane; Flachowsky, Gerhard

    2007-08-01

    The nutritive value of genetically modified myristic acid-rich rapeseed, in which a acyl-thioesterase gene inserted, was studied. Crude nutrients, amino acid and fatty acid profiles as well as mineral and glucosinolate contents were determined and compared with those of the non-transgenic parental cultivar. The concentration of crude nutrients, minerals and amino acids were found to be within the range of natural variance. The myristic and palmitic acid content increased from 0.1 - 11.4% and from 3.6-20%, respectively, at the expense of oleic acid, which decreased from 68.6-42.6% of total fatty acids. The glucosinolate contents increased from 12.4 micromol/g in the parental plant to 19 micromol/g DM in the GM-plant. Full-fat rapeseed of both cultivars was incorporated in pig diets at a level of 15%, and the digestibility and the production efficiency were tested under ad libitum feeding conditions with ten pigs each over the growing finishing period from 32-105 kg BW. The experimental diets did not show significant differences in digestibility and energetic feeding value. However, feed intake and weight gain decreased presumably due to the increasing glucosinolate intake associated with the feeding of transgenic rapeseed. The dietary fatty acids profile influenced the fatty acid profile of body fat. Myristic acid accumulated in back fat and intramuscular fat while the oleic acid content decreased. The increased glucosinolate intake affected the weight of thyroid glands and their iodine concentration.

  10. Real-time augmentation of USDA yield grade application to beef carcasses using video image analysis.

    PubMed

    Steiner, R; Wyle, A M; Vote, D J; Belk, K E; Scanga, J A; Wise, J W; Tatum, J D; Smith, G C

    2003-09-01

    In two phases, this study assessed the ability of two video image analysis (VIA) instruments, VIASCAN and Computer Vision System (CVS), to augment assignment of yield grades (YG) to beef carcasses to 0.1 of a YG at commercial packing plant speeds and to test cutout prediction accuracy of a YG augmentation system that used a prototype augmentation touchpanel grading display (designed to operate commercially in real-time). In Phase I, beef carcasses (n = 505) were circulated twice at commercial chain speeds (340 carcasses per hour) by 12 on-line USDA graders. During the first pass, on-line graders assigned a whole-number YG and a quality grade (QG) to carcasses as they would normally. During the second pass, on-line graders assigned only adjusted preliminary yield grades (APYG) and QG to carcasses, whereas the two VIA instruments measured the longissimus muscle area (LMA) of each carcass. Kidney, pelvic, and heart fat (KPH) was removed and weighed to allow computation of actual KPH percentage. Those traits were compared to the expert YG and expert YG factors. On-line USDA graders' APYG were closely related (r = 0.83) to expert APYG. Instrument-measured LMA were closely related (r = 0.88 and 0.94; mean absolute error = 0.3 and 0.2 YG units, for VIASCAN and CVS, respectively) to expert LMA. When YG were augmented using instrument-measured LMA and computed either including or neglecting actual KPH percentage, YG were closely related (r = 0.93 and 0.92, mean absolute error = 0.32 and 0.40 YG units, respectively, using VIASCAN-measured LMA; r = 0.95 and 0.94, mean absolute error = 0.24 and 0.34 YG units, respectively, using CVS-measured LMA) to expert YG. In Phase II, augmented YG were assigned (0.1 of a YG) to beef carcasses (n = 290) at commercial chain speeds using VIASCAN and CVS to determine LMA, whereas APYG and QG were determined by online graders via a touch-panel display. On-line grader YG (whole-number), expert grader YG (to the nearest 0.1 of a YG), and VIASCAN

  11. A highly acid-resistant novel strain of Lactobacillus johnsonii No. 1088 has antibacterial activity, including that against Helicobacter pylori, and inhibits gastrin-mediated acid production in mice

    PubMed Central

    Aiba, Yuji; Nakano, Yasuhiro; Koga, Yasuhiro; Takahashi, Kenji; Komatsu, Yasuhiko

    2015-01-01

    A novel strain of Lactobacillus johnsonii No. 1088 was isolated from the gastric juice of a healthy Japanese male volunteer, and characterized for its effectiveness in the stomach environment. Lactobacillus johnsonii No. 1088 was found to have the strongest acid resistance among several lactobacilli examined (>10% of cells survived at pH 1.0 after 2 h), and such a high acid resistance property was a specific characteristic of this strain of L. johnsonii. When cultured with various virulent bacteria, L. johnsonii No. 1088 inhibited the growth of Helicobacter pylori,Escherichia coli O-157, Salmonella Typhimurium, and Clostridium difficile, in which case its effectiveness was more potent than that of a type strain of L. johnsonii,JCM2012. In addition to its effect in vitro, L. johnsonii No. 1088 inhibited the growth of H. pylori in human intestinal microbiota-associated mice in both its live and lyophilized forms. Moreover, L. johnsonii No. 1088 suppressed gastric acid secretion in mice via decreasing the number of gastrin-positive cells in the stomach. These results taken together suggest that L. johnsonii No. 1088 is a unique lactobacillus having properties beneficial for supporting H. pylori eradication by triple therapy including the use of a proton pump inhibitor (PPI) and also for prophylaxis of gastroesophageal reflux disease possibly caused after H. pylori eradication as a side effect of PPI. PMID:25771812

  12. A highly acid-resistant novel strain of Lactobacillus johnsonii No. 1088 has antibacterial activity, including that against Helicobacter pylori, and inhibits gastrin-mediated acid production in mice.

    PubMed

    Aiba, Yuji; Nakano, Yasuhiro; Koga, Yasuhiro; Takahashi, Kenji; Komatsu, Yasuhiko

    2015-06-01

    A novel strain of Lactobacillus johnsonii No. 1088 was isolated from the gastric juice of a healthy Japanese male volunteer, and characterized for its effectiveness in the stomach environment. Lactobacillus johnsonii No. 1088 was found to have the strongest acid resistance among several lactobacilli examined (>10% of cells survived at pH 1.0 after 2 h), and such a high acid resistance property was a specific characteristic of this strain of L. johnsonii. When cultured with various virulent bacteria, L. johnsonii No. 1088 inhibited the growth of Helicobacter pylori, Escherichia coli O-157, Salmonella Typhimurium, and Clostridium difficile, in which case its effectiveness was more potent than that of a type strain of L. johnsonii, JCM2012. In addition to its effect in vitro, L. johnsonii No. 1088 inhibited the growth of H. pylori in human intestinal microbiota-associated mice in both its live and lyophilized forms. Moreover, L. johnsonii No. 1088 suppressed gastric acid secretion in mice via decreasing the number of gastrin-positive cells in the stomach. These results taken together suggest that L. johnsonii No. 1088 is a unique lactobacillus having properties beneficial for supporting H. pylori eradication by triple therapy including the use of a proton pump inhibitor (PPI) and also for prophylaxis of gastroesophageal reflux disease possibly caused after H. pylori eradication as a side effect of PPI.

  13. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA)

    PubMed Central

    Li, Huinan; Wu, Cheng; Aramayo, Rodolfo; Sachs, Matthew S.; Harlow, Mark L.

    2015-01-01

    Synaptic vesicles (SVs) are neuronal presynaptic organelles that load and release neurotransmitter at chemical synapses. In addition to classic neurotransmitters, we have found that synaptic vesicles isolated from the electric organ of Torpedo californica, a model cholinergic synapse, contain small ribonucleic acids (sRNAs), primarily the 5′ ends of transfer RNAs (tRNAs) termed tRNA fragments (trfRNAs). To test the evolutionary conservation of SV sRNAs we examined isolated SVs from the mouse central nervous system (CNS). We found abundant levels of sRNAs in mouse SVs, including trfRNAs and micro RNAs (miRNAs) known to be involved in transcriptional and translational regulation. This discovery suggests that, in addition to inducing changes in local dendritic excitability through the release of neurotransmitters, SVs may, through the release of specific trfRNAs and miRNAs, directly regulate local protein synthesis. We believe these findings have broad implications for the study of chemical synaptic transmission. PMID:26446566

  14. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA).

    PubMed

    Li, Huinan; Wu, Cheng; Aramayo, Rodolfo; Sachs, Matthew S; Harlow, Mark L

    2015-01-01

    Synaptic vesicles (SVs) are neuronal presynaptic organelles that load and release neurotransmitter at chemical synapses. In addition to classic neurotransmitters, we have found that synaptic vesicles isolated from the electric organ of Torpedo californica, a model cholinergic synapse, contain small ribonucleic acids (sRNAs), primarily the 5' ends of transfer RNAs (tRNAs) termed tRNA fragments (trfRNAs). To test the evolutionary conservation of SV sRNAs we examined isolated SVs from the mouse central nervous system (CNS). We found abundant levels of sRNAs in mouse SVs, including trfRNAs and micro RNAs (miRNAs) known to be involved in transcriptional and translational regulation. This discovery suggests that, in addition to inducing changes in local dendritic excitability through the release of neurotransmitters, SVs may, through the release of specific trfRNAs and miRNAs, directly regulate local protein synthesis. We believe these findings have broad implications for the study of chemical synaptic transmission.

  15. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA).

    PubMed

    Li, Huinan; Wu, Cheng; Aramayo, Rodolfo; Sachs, Matthew S; Harlow, Mark L

    2015-01-01

    Synaptic vesicles (SVs) are neuronal presynaptic organelles that load and release neurotransmitter at chemical synapses. In addition to classic neurotransmitters, we have found that synaptic vesicles isolated from the electric organ of Torpedo californica, a model cholinergic synapse, contain small ribonucleic acids (sRNAs), primarily the 5' ends of transfer RNAs (tRNAs) termed tRNA fragments (trfRNAs). To test the evolutionary conservation of SV sRNAs we examined isolated SVs from the mouse central nervous system (CNS). We found abundant levels of sRNAs in mouse SVs, including trfRNAs and micro RNAs (miRNAs) known to be involved in transcriptional and translational regulation. This discovery suggests that, in addition to inducing changes in local dendritic excitability through the release of neurotransmitters, SVs may, through the release of specific trfRNAs and miRNAs, directly regulate local protein synthesis. We believe these findings have broad implications for the study of chemical synaptic transmission. PMID:26446566

  16. A nopA deletion mutant of Sinorhizobium fredii USDA257, a soybean symbiont, is impaired in nodulation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sinorhizobium fredii USDA257 employs type III secretion system (T3SS) to deliver effector proteins into the host cells through filamentous surface appendages, called pili. The NopA protein is the major component of USDA257 pili. The promoter region of USDA257 nopA posses a well conserved tts box. Se...

  17. 7 CFR 170.12 - What are the selection criteria for participation in the USDA Farmers Market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the USDA Farmers Market? 170.12 Section 170.12 Agriculture Regulations of the Department of... OF 1946 USDA FARMERS MARKET § 170.12 What are the selection criteria for participation in the USDA Farmers Market? The selection criteria are designed to ensure a consistently high level of quality...

  18. 7 CFR 1.215 - Subpoenas duces tecum for USDA records in judicial or administrative proceedings in which the...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Subpoenas duces tecum for USDA records in judicial or... the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Appearance of USDA Employees as Witnesses in Judicial or Administrative Proceedings § 1.215 Subpoenas duces tecum for USDA records in judicial...

  19. 2 CFR 417.222 - How would the exclusions from coverage for USDA's export credit guarantee and direct credit...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... USDA's export credit guarantee and direct credit programs apply? 417.222 Section 417.222 Grants and... DEBARMENT AND SUSPENSION Covered Transactions § 417.222 How would the exclusions from coverage for USDA's... guarantee issued by the USDA to a U.S. exporter. The U.S. exporter usually assigns the guarantee to a...

  20. 2 CFR 417.222 - How would the exclusions from coverage for USDA's export credit guarantee and direct credit...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... USDA's export credit guarantee and direct credit programs apply? 417.222 Section 417.222 Grants and... DEBARMENT AND SUSPENSION Covered Transactions § 417.222 How would the exclusions from coverage for USDA's... guarantee issued by the USDA to a U.S. exporter. The U.S. exporter usually assigns the guarantee to a...

  1. 7 CFR 1.215 - Subpoenas duces tecum for USDA records in judicial or administrative proceedings in which the...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Subpoenas duces tecum for USDA records in judicial or... the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Appearance of USDA Employees as Witnesses in Judicial or Administrative Proceedings § 1.215 Subpoenas duces tecum for USDA records in judicial...

  2. 2 CFR 417.222 - How would the exclusions from coverage for USDA's export credit guarantee and direct credit...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... USDA's export credit guarantee and direct credit programs apply? 417.222 Section 417.222 Grants and... DEBARMENT AND SUSPENSION Covered Transactions § 417.222 How would the exclusions from coverage for USDA's... guarantee issued by the USDA to a U.S. exporter. The U.S. exporter usually assigns the guarantee to a...

  3. 7 CFR 1.215 - Subpoenas duces tecum for USDA records in judicial or administrative proceedings in which the...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Subpoenas duces tecum for USDA records in judicial or... the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Appearance of USDA Employees as Witnesses in Judicial or Administrative Proceedings § 1.215 Subpoenas duces tecum for USDA records in judicial...

  4. 7 CFR 1.215 - Subpoenas duces tecum for USDA records in judicial or administrative proceedings in which the...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Subpoenas duces tecum for USDA records in judicial or... the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Appearance of USDA Employees as Witnesses in Judicial or Administrative Proceedings § 1.215 Subpoenas duces tecum for USDA records in judicial...

  5. 2 CFR 417.222 - How would the exclusions from coverage for USDA's export credit guarantee and direct credit...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... USDA's export credit guarantee and direct credit programs apply? 417.222 Section 417.222 Grants and... DEBARMENT AND SUSPENSION Covered Transactions § 417.222 How would the exclusions from coverage for USDA's... guarantee issued by the USDA to a U.S. exporter. The U.S. exporter usually assigns the guarantee to a...

  6. 7 CFR 1.215 - Subpoenas duces tecum for USDA records in judicial or administrative proceedings in which the...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Subpoenas duces tecum for USDA records in judicial or... the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Appearance of USDA Employees as Witnesses in Judicial or Administrative Proceedings § 1.215 Subpoenas duces tecum for USDA records in judicial...

  7. Simulated instrument augmentation of USDA yield grade application to beef carcasses.

    PubMed

    Belk, K E; Scanga, J A; Tatum, J D; Wise, J W; Smith, G C

    1998-02-01

    Because no instrument technology has been shown to predict beef carcass composition better than USDA yield grades, this study was conducted to determine whether an instrument could be used to augment and improve the accuracy of USDA yield grade placement. Adjusted preliminary yield grade (PYG), ribeye area (REA), estimated percentage of kidney, pelvic, and heart fat (KPH), hot carcass weight (HCW), and USDA yield grade (called and computed) were determined by five on-line USDA graders and two USDA grading supervisors for beef carcasses (n = 550) selected randomly in a commercial beef packing plant. Data were compared (2,737 comparisons) to Gold Standard yield grades and yield grade factors determined by an expert panel of carcass evaluators (unrestrained in access or time to evaluate carcasses). On-line USDA grader PYG were closely related (mean absolute error of .15 +/- .14 yield grade units; r = .91), and on-line REA and KPH were nominally related (mean absolute error of .51 +/- .35, .06 +/- .07 yield grade units and r = .48 and .66, respectively), to Gold Standard yield grade factors. On-line USDA graders determined adjusted PYG effectively, but they may require instrument assistance to evaluate carcass muscling traits and perform time-sensitive computations. To explain why instrument technology may not estimate beef carcass fatness as accurately as USDA yield grades, the absolute mean difference between Gold Standard measured PYG and adjusted PYG were compared. Only 5.6% of the sample population required no PYG adjustment, 94.4% required some adjustment, and 11.0% required over a .5 yield grade unit adjustment. Yield grades for beef carcasses, called by the USDA graders and supervisors at chain speeds, resulted in greater accuracy (absolute mean error of .24 +/- .43 yield grade units; r = .82) than when yield grades were computed for carcasses using the yield grade factors determined by on-line USDA graders and supervisors at chain speeds (absolute mean error

  8. Serous tubal intraepithelial carcinoma upregulates markers associated with high-grade serous carcinomas including Rsf-1 (HBXAP), cyclin E and fatty acid synthase.

    PubMed

    Sehdev, Ann Smith; Kurman, Robert J; Kuhn, Elisabetta; Shih, Ie-Ming

    2010-06-01

    Serous tubal intraepithelial carcinoma (STIC) has been proposed as a precursor for many pelvic high-grade serous carcinomas. Our previous analysis of the ovarian cancer genome identified several genes with oncogenic potential that are amplified and/or overexpressed in the majority of high-grade serous carcinomas. Determining whether these genes are upregulated in STICs is important in further elucidating the relationship of STICs to high-grade serous carcinomas and is fundamental in understanding the molecular pathogenesis of high-grade serous carcinomas. In this study, 37 morphologically defined STICs were obtained from 23 patients with stage IIIC/IV high-grade serous carcinomas. Both STICs and the high-grade serous carcinomas were analyzed for expression of Rsf-1 (HBXAP), cyclin E, fatty acid synthase (FASN) and mucin-4. In addition, they were examined for expression of established markers including p53, Ki-67 and p16. We found that diffuse nuclear p53 and p16 immunoreactivity was observed in 27 (75%) of 36 and 18 (55%) of 33 STICs, respectively, whereas an elevated Ki-67 labeling index (>or=10%) was detected in 29 (78%) of 37 STICs. Cyclin E nuclear staining was seen in 24 (77%) of 35 STICs, whereas normal tubal epithelial cells were all negative. Increased Rsf-1 and FASN immunoreactivity occurred in 63%, and 62% of STICs, respectively, compared with adjacent normal-appearing tubal epithelium. Interestingly, only one STIC showed increased mucin-4 immunoreactivity. Carcinomas, when compared with STICs, overexpressed p16, Rsf-1, cyclin E and FASN in a higher proportion of cases. In conclusion, STICs express several markers including Rsf-1, cyclin E and FASN in high-grade serous carcinomas. In contrast, mucin-4 immunoreactivity either did not change or was reduced in most STICs. These results suggest that overexpression of Rsf-1, cyclin E and FASN occurs early in tumor progression.

  9. USDA Snack Policy Implementation: Best Practices From the Front Lines, United States, 2013–2014

    PubMed Central

    Chriqui, Jamie; Chavez, Noel; Odoms-Young, Angela; Handler, Arden

    2016-01-01

    Introduction The Smart Snacks in Schools interim final rule was promulgated by the US Department of Agriculture (USDA) as authorized by the Healthy, Hunger-Free Kids Act of 2010 (PL 111–296) and implementation commenced beginning July 1, 2014; however, in the years leading up to this deadline, national studies suggested that most schools were far from meeting the USDA standards. Evidence to guide successful implementation of the standards is needed. This study examined snack policy implementation in exemplary high schools to learn best practices for implementation. Methods Guided by a multiple case study approach, school professionals (n = 37) from 9 high schools across 8 states were recruited to be interviewed about perceptions of school snack implementation; schools were selected using criterion sampling on the basis of the HealthierUS Schools Challenge: Smarter Lunchrooms (HUSSC: SL) database. Interview transcripts and internal documents were organized and coded in ATLAS.Ti v7; 2 researchers coded and analyzed data using a constant comparative analysis method to identify best practice themes. Results Best practices for snack policy implementation included incorporating the HUSSC: SL award’s comprehensive wellness approach; leveraging state laws or district policies to reinforce snack reform initiatives; creating strong internal and external partnerships; and crafting positive and strategic communications. Conclusion Implementation of snack policies requires evidence of successful experiences from those on the front lines. As federal, state, and local technical assistance entities work to ensure implementation of the Smart Snacks standards, these best practices provide strategies to facilitate the process. PMID:27309416

  10. Enzymatic preparation of optically pure (+)-2-azabicyclo[2.2.1]hept-5-en-3-one by (-)-γ-lactamase from Bradyrhizobium japonicum USDA 6.

    PubMed

    Zhu, Shaozhou; Ren, Lu; Yu, Songzhu; Gong, Cuiyu; Song, Dawei; Zheng, Guojun

    2014-10-15

    Whole cells of Bradyrhizobium japonicum USDA 6 showed both (+)-γ-lactamase activity and (-)-γ-lactamase activity. Insight into the genome of B. japonicum USDA 6 revealed two potential γ-lactamases: a type I (+)-γ-lactamase and a (-)-γ-lactamase, making it the first strain to contain two totally different enantioselective lactamases. Both recombinant enzymes could easily be used to prepare either optically pure (+)-γ-lactam ((+)-2-azabicyclo[2.2.1]hept-5-en-3-one) or optically pure (-)-γ-lactam ((-)-2-azabicyclo[2.2.1]hept-5-en-3-one), which are versatile synthetic building blocks for the synthesis of various carbocyclic nucleosides and carbocyclic sugar analogues. Bioinformatic analysis showed that the type I (+)-γ-lactamase belongs to the amidase signature family, with 504 amino acids; the (-)-γ-lactamase, which consists of 274 amino acids, belongs to the hydrolase family. Here, we report that B. japonicum USDA contains a (-)-γ-lactamase in addition to a (+)-γ-lactamase, and it is the (-)-γ-lactamase from this strain that is examined in detail in this Letter. Enzymatic synthesis of optically pure (+)-γ-lactam with nearly 50% isolated yield and >99% ee was achieved.

  11. Metabolic evidence of vitamin B-12 deficiency, including high homocysteine and methylmalonic acid and low holotranscobalamin, is more pronounced in older adults with elevated plasma folate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: An analysis of data from the National Health and Nutrition Examination Survey indicated that in older adults exposed to folic acid fortification, the combination of low serum vitamin B-12 and elevated folate is associated with higher concentrations of homocysteine and methylmalonic acid ...

  12. 75 FR 29311 - Solicitation of Nominations for Members of the USDA Grain Inspection Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-25

    ..., 2009, GIPSA published a notice in the Federal Register (74 FR 57991) soliciting nominations to serve on... Grain Inspection, Packers and Stockyards Administration Solicitation of Nominations for Members of the USDA Grain Inspection Advisory Committee AGENCY: Grain Inspection, Packers and...

  13. AmeriFlux US-AR2 ARM USDA UNL OSU Woodward Switchgrass 2

    SciTech Connect

    Billesbach, Dave; Bradford, James

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site US-AR2 ARM USDA UNL OSU Woodward Switchgrass 2. Site Description - The ARM USDA UNL OSU Woodward Switchgrass 2 tower is located on public land owned by the USDA-ARS Southern Plains Range Research Station in Woodward, Oklahoma. The site is on a former wheat field that is in the process of changing to switchgrass. A companion site (ARM USDA UNL OSU Woodward Switchgrass 1) is on a former native prairie. Previous wheat was planted in Fall 2008. In Spring 2009, herbicide was applied to kill the wheat prior to switchgrass planting. Later in the year, the site was sprayed with post-emergence herbicide. In 2010, fertilization occurred before herbicide was sprayed for broadleaf control.

  14. Intergeneric hybrids in Rosaceae subtribe Pyrinae (formerly subfamily Maloideae) at USDA genebank

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Agricultural Research Service maintains clonal germplasm collections representing world diversity of Pyrus, Cydonia and Mespilus at its National Clonal Germplasm Repository in Corvallis, Oregon, USA. Smaller collections of Amelanchier, Aronia, Crataegus, Sorbus and other genera in Rosaceae ...

  15. AmeriFlux US-AR1 ARM USDA UNL OSU Woodward Switchgrass 1

    SciTech Connect

    Billesbach, Dave; Bradford, James

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site US-AR1 ARM USDA UNL OSU Woodward Switchgrass 1. Site Description - The ARM USDA UNL OSU Woodward Switchgrass 1 tower is located on public land owned by the USDA-ARS Southern Plains Range Research Station in Woodward, Oklahoma. The site is on a former native prairie that is in the process of changing to switchgrass. A second companion site (ARM USDA UNL OSU Woodward Switchgrass 2) is on a former wheat field. In Spring 2009, the former native prairie site was burned, cattle were put on the pasture to graze down emergent grass, and broadleaf herbicide was sprayed. In Summer 2009, the cattle were removed from the pasture, and the site was sprayed with herbicide to kill all grass. In Spring 2010, prior to the planting of switchgrass, final herbicide was sprayed to kill cheat grass and to control broadleaf plants.

  16. 7 CFR 170.2 - Is the USDA Farmers Market a producer-only market?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... fruits, vegetables, flowers, bedding plants, and potted plants. The USDA Farmers Market is a producer-only market since only farmers who may sell products that they grow or produce will be selected...

  17. 7 CFR 170.2 - Is the USDA Farmers Market a producer-only market?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... fruits, vegetables, flowers, bedding plants, and potted plants. The USDA Farmers Market is a producer-only market since only farmers who may sell products that they grow or produce will be selected...

  18. 7 CFR 170.13 - What are the operating guidelines for the USDA Farmers Market?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS... for all items being sold. (e) Clean-up. Participants are responsible for cleaning all trash and...

  19. PHOTOCOPY OF STANDARD USDA/USFS PLAN FOR 13' X 13' STEEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PHOTOCOPY OF STANDARD USDA/USFS PLAN FOR 13' X 13' STEEL LOOKOUT HOUSE (CAB); ELEVATIONS, SECTIONS, MISC. DETAILS; DATED 1961 - North Mountain Lookout, Stanislaus National Forest, Groveland, Tuolumne County, CA

  20. 7 CFR 15f.3 - If I want to use these procedures to have USDA consider my complaint, how long will it take for...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false If I want to use these procedures to have USDA consider my complaint, how long will it take for USDA to make a decision? 15f.3 Section 15f.3 Agriculture... USDA consider my complaint, how long will it take for USDA to make a decision? To the maximum...

  1. 7 CFR 15f.3 - If I want to use these procedures to have USDA consider my complaint, how long will it take for...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false If I want to use these procedures to have USDA consider my complaint, how long will it take for USDA to make a decision? 15f.3 Section 15f.3 Agriculture... USDA consider my complaint, how long will it take for USDA to make a decision? To the maximum...

  2. 7 CFR 15f.3 - If I want to use these procedures to have USDA consider my complaint, how long will it take for...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false If I want to use these procedures to have USDA consider my complaint, how long will it take for USDA to make a decision? 15f.3 Section 15f.3 Agriculture... USDA consider my complaint, how long will it take for USDA to make a decision? To the maximum...

  3. 7 CFR 15f.3 - If I want to use these procedures to have USDA consider my complaint, how long will it take for...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false If I want to use these procedures to have USDA consider my complaint, how long will it take for USDA to make a decision? 15f.3 Section 15f.3 Agriculture... USDA consider my complaint, how long will it take for USDA to make a decision? To the maximum...

  4. 7 CFR 15f.3 - If I want to use these procedures to have USDA consider my complaint, how long will it take for...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false If I want to use these procedures to have USDA consider my complaint, how long will it take for USDA to make a decision? 15f.3 Section 15f.3 Agriculture... USDA consider my complaint, how long will it take for USDA to make a decision? To the maximum...

  5. Final work plan : Phase I investigation of potential contamination at the former CCC/USDA grain storage facility in Savannah, Missouri.

    SciTech Connect

    LaFreniere, L. M.; Environmental Science Division

    2007-10-12

    . This work will be performed in accord with the Intergovernmental Agreement established between the Farm Service Agency of the USDA and MoDNR, to address carbon tetrachloride contamination potentially associated with a number of former CCC/USDA grain storage facilities in Missouri. The investigative activities at Savannah will be conducted on behalf of the CCC/USDA by the Environmental Science Division of Argonne National Laboratory. Argonne is a nonprofit, multidisciplinary research center operated by UChicago Argonne, LLC, for the U.S. Department of Energy (DOE). The CCC/USDA has entered into an agreement with the DOE, under which Argonne provides technical assistance to the CCC/USDA with environmental site characterization and remediation at its former grain storage facilities. The site characterization at Savannah will take place in phases. This approach is recommended by the CCC/USDA and Argonne, so that information obtained and interpretations developed during each incremental stage of the investigation can be used most effectively to guide subsequent phases of the program. This site-specific Work Plan outlines the specific technical objectives and scope of work proposed for Phase I of the Savannah investigation. This Work Plan also includes the community relations plan to be followed throughout the CCC/USDA program at the Savannah site. Argonne is developing a Master Work Plan specific to operations in the state of Missouri. In the meantime, Argonne will issue a Provisional Master Work Plan (PMWP; Argonne 2007) that will be submitted to the MoDNR for review and approval. The agency has already reviewed and approved (with minor changes) the present Master Work Plan (Argonne 2002) under which Argonne currently operates in Kansas. The PMWP (Argonne 2007) will provide detailed information and guidance on the investigative technologies, analytical methodologies, quality assurance-quality control measures, and general health and safety policies to be employed by

  6. Temporal trends of perfluoroalkane sulfonic acids and their sulfonamide-based precursors in herring from the Swedish west coast 1991-2011 including isomer-specific considerations.

    PubMed

    Ullah, Shahid; Huber, Sandra; Bignert, Anders; Berger, Urs

    2014-04-01

    A method was developed for simultaneous analysis of perfluoroalkane sulfonic acids (PFSAs) and their sulfonamide-based precursors (perfluoroalkane sulfonamidoacetic acids (FASAAs), sulfonamides (FASAs), and sulfonamidoethanols (FASEs)) in fish muscle. Extraction was performed with acetonitrile followed by a clean-up and fractionation step and instrumental analysis by UPLC/MS/MS and GC/MS. Time trends of PFSAs and their precursors in herring muscle samples originating from the Kattegat at the west coast of Sweden were investigated covering the years 1991-2011. The following analytes were detected, all with decreasing or unchanged trends between 1991 and 2011: Perfluorobutane sulfonic acid (PFBS, below the method detection limit (acid (PFHxS, 9-38pg/g), perfluorooctane sulfonic acid (PFOS, 240-930pg/g), perfluorodecane sulfonic acid (PFDS, acid (MeFOSAA and EtFOSAA, 2-39 and 2-31pg/g, respectively) and perfluorooctane sulfonamide (FOSA, 78-920pg/g). The highest concentrations were found for PFOS and FOSA around the turn of the century. Shorter disappearance half-lives were observed for precursors compared to PFSAs. Assuming that these trend differences are representative for fish consumed by the general Swedish population, this observation suggests that the relative contribution of precursors to total human exposure to PFOS via fish intake has decreased in Sweden over the study period. PFOS precursors in fish may have constituted a significant indirect exposure pathway for PFOS in the 1990s. Isomer-specific analysis of PFOS and FOSA revealed <10% relative contributions of branched isomers to total PFOS and total FOSA. Furthermore, the percentage branched isomers decreased over time for both compounds. These findings are contrary to patterns and temporal trends of PFOS isomers commonly found in human serum. In combination with literature data on isomer patterns

  7. Arsenic Metabolites, Including N-Acetyl-4-hydroxy-m-arsanilic Acid, in Chicken Litter from a Roxarsone-Feeding Study Involving 1600 Chickens.

    PubMed

    Yang, Zonglin; Peng, Hanyong; Lu, Xiufen; Liu, Qingqing; Huang, Rongfu; Hu, Bin; Kachanoski, Gary; Zuidhof, Martin J; Le, X Chris

    2016-07-01

    The poultry industry has used organoarsenicals, such as 3-nitro-4-hydroxyphenylarsonic acid (Roxarsone, ROX), to prevent disease and to promote growth. Although previous studies have analyzed arsenic species in chicken litter after composting or after application to agricultural lands, it is not clear what arsenic species were excreted by chickens before biotransformation of arsenic species during composting. We describe here the identification and quantitation of arsenic species in chicken litter repeatedly collected on days 14, 24, 28, 30, and 35 of a Roxarsone-feeding study involving 1600 chickens of two strains. High performance liquid chromatography separation with simultaneous detection by both inductively coupled plasma mass spectrometry and electrospray ionization tandem mass spectrometry provided complementary information necessary for the identification and quantitation of arsenic species. A new metabolite, N-acetyl-4-hydroxy-m-arsanilic acid (N-AHAA), was identified, and it accounted for 3-12% of total arsenic. Speciation analyses of litter samples collected from ROX-fed chickens on days 14, 24, 28, 30, and 35 showed the presence of N-AHAA, 3-amino-4-hydroxyphenylarsonic acid (3-AHPAA), inorganic arsenite (As(III)), arsenate (As(V)), monomethylarsonic acid (MMA(V)), dimethylarsinic acid (DMA(V)), and ROX. 3-AHPAA accounted for 3-19% of the total arsenic. Inorganic arsenicals (the sum of As(III) and As(V)) comprised 2-6% (mean 3.5%) of total arsenic. Our results on the detection of inorganic arsenicals, methylarsenicals, 3-AHPAA, and N-AHAA in the chicken litter support recent findings that ROX is actually metabolized by the chicken or its gut microbiome. The presence of the toxic metabolites in chicken litter is environmentally relevant as chicken litter is commonly used as fertilizer.

  8. Effect of including carob pulp in the diet of fattening pigs on the fatty acid composition and oxidative stability of pork.

    PubMed

    Inserra, L; Luciano, G; Bella, M; Scerra, M; Cilione, C; Basile, P; Lanza, M; Priolo, A

    2015-02-01

    The effect of feeding pigs with carob pulp on meat quality was investigated. Nine pigs were finished on a conventional concentrate-based diet (control), while two groups received a diet comprising of the same ingredients with the inclusion of 8% or 15% carob pulp (Carob 8% and Carob 15%, respectively). Feeding carob-containing diets reduced the concentration of saturated fatty acids in the muscle, increased the concentration of monounsaturated fatty acids in meat (P < 0.01) and of n-3 polyunsaturated fatty acids (PUFAs) and reduced the n-6/n-3 PUFA ratio (P < 0.001). The meat underwent slow oxidative deterioration over 9 days of storage. However, the Carob 15% treatment increased meat susceptibility to lipid oxidation across storage (P = 0.03), while the dietary treatment did not affect meat colour stability. In conclusion, feeding pigs with carob pulp could represent a strategy,in the Mediterranean areas, to naturally improve meat nutritional value and to promote the exploitation of this local feed resource.

  9. Effect of including carob pulp in the diet of fattening pigs on the fatty acid composition and oxidative stability of pork.

    PubMed

    Inserra, L; Luciano, G; Bella, M; Scerra, M; Cilione, C; Basile, P; Lanza, M; Priolo, A

    2015-02-01

    The effect of feeding pigs with carob pulp on meat quality was investigated. Nine pigs were finished on a conventional concentrate-based diet (control), while two groups received a diet comprising of the same ingredients with the inclusion of 8% or 15% carob pulp (Carob 8% and Carob 15%, respectively). Feeding carob-containing diets reduced the concentration of saturated fatty acids in the muscle, increased the concentration of monounsaturated fatty acids in meat (P < 0.01) and of n-3 polyunsaturated fatty acids (PUFAs) and reduced the n-6/n-3 PUFA ratio (P < 0.001). The meat underwent slow oxidative deterioration over 9 days of storage. However, the Carob 15% treatment increased meat susceptibility to lipid oxidation across storage (P = 0.03), while the dietary treatment did not affect meat colour stability. In conclusion, feeding pigs with carob pulp could represent a strategy,in the Mediterranean areas, to naturally improve meat nutritional value and to promote the exploitation of this local feed resource. PMID:25460134

  10. The influence of sex-class, USDA yield grade and USDA quality grade on seam fat trim from the primals of beef carcasses.

    PubMed

    Jones, D K; Savell, J W; Cross, H R

    1990-07-01

    Beef carcasses (129 steers and 80 heifers) differing in weight, muscling, fatness and marbling score were selected to represent the full spectrum of USDA yield grades; one side was fabricated into boneless primal cuts. Primals were trimmed of all external fat and intermuscular (seam) fat and all components were weighed. Regression equations were developed to predict the percentage of seam fat on an external fat-free primal basis using USDA yield grade (YG), marbling score and a squared function of YG as the independent variables. YG (.77) and marbling score (.67) were highly correlated to seam fat. Heifers tended to have a higher predicted percentage of seam fat than did steers across all YG. Primals from USDA Choice carcasses had approximately 1.0 percentage point more predicted seam fat than did USDA Select primals at the same YG and sex-class. The YG 2.5 heifers had similar proportions of predicted seam fat from primals as YG 3.5 steers, but YG 3.5 heifers tended to have more seam fat than YG 4.5 steers. The same trend was noted between YG 4.5 heifers and YG 5.5 steers, indicating a sex-related deposition of seam fat in fed cattle.

  11. Final work plan : phase I investigation of potential contamination at the former CCC/USDA grain storage facility in Montgomery City, Missouri.

    SciTech Connect

    LaFreniere, L. M.; Environmental Science Division

    2010-08-16

    former grain storage facility, the CCC/USDA will conduct investigations to (1) characterize the source(s), extent, and factors controlling the possible subsurface distribution and movement of carbon tetrachloride at the Montgomery City site and (2) evaluate the health and environmental threats potentially represented by the contamination. This work will be performed in accord with the Intergovernmental Agreement established between the Farm Service Agency of the USDA and the MoDNR, to address carbon tetrachloride contamination potentially associated with a number of former CCC/USDA grain storage facilities in Missouri. The investigations at Montgomery City will be conducted on behalf of the CCC/USDA by the Environmental Science Division of Argonne National Laboratory. Argonne is a nonprofit, multidisciplinary research center operated by UChicago Argonne, LLC, for the U.S. Department of Energy (DOE). The CCC/USDA has entered into an agreement with DOE, under which Argonne provides technical assistance to the CCC/USDA with environmental site characterization and remediation at its former grain storage facilities. The site characterization at Montgomery City will take place in phases. This approach is recommended by the CCC/USDA and Argonne, so that information obtained and interpretations developed during each incremental stage of the investigation can be used most effectively to guide subsequent phases of the program. This site-specific Work Plan outlines the specific technical objectives and scope of work proposed for Phase I of the Montgomery City investigation. This Work Plan also includes the community relations plan to be followed throughout the CCC/USDA program at the Montgomery City site. Argonne is developing a Master Work Plan specific to operations in the state of Missouri. In the meantime, Argonne has issued a Provisional Master Work Plan (PMWP; Argonne 2007) that has been reviewed and approved by the MoDNR for current use. The PMWP (Argonne 2007) provides

  12. Retrospective Analog Year Analyses Using NASA Satellite Data to Improve USDA's World Agricultural Supply and Demand Estimates

    NASA Technical Reports Server (NTRS)

    Teng, William; Shannon, Harlan

    2011-01-01

    The USDA World Agricultural Outlook Board (WAOB) is responsible for monitoring weather and climate impacts on domestic and foreign crop development. One of WAOB's primary goals is to determine the net cumulative effect of weather and climate anomalies on final crop yields. To this end, a broad array of information is consulted, including maps, charts, and time series of recent weather, climate, and crop observations; numerical output from weather and crop models; and reports from the press, USDA attach s, and foreign governments. The resulting agricultural weather assessments are published in the Weekly Weather and Crop Bulletin, to keep farmers, policy makers, and commercial agricultural interests informed of weather and climate impacts on agriculture. Because both the amount and timing of precipitation significantly affect crop yields, WAOB often uses precipitation time series to identify growing seasons with similar weather patterns and help estimate crop yields for the current growing season, based on observed yields in analog years. Historically, these analog years are visually identified; however, the qualitative nature of this method sometimes precludes the definitive identification of the best analog year. Thus, one goal of this study is to derive a more rigorous, statistical approach for identifying analog years, based on a modified coefficient of determination, termed the analog index (AI). A second goal is to compare the performance of AI for time series derived from surface-based observations vs. satellite-based measurements (NASA TRMM and other data).

  13. Suppression of the ELO-2 FA elongation activity results in alterations of the fatty acid composition and multiple physiological defects, including abnormal ultradian rhythms, in Caenorhabditis elegans.

    PubMed Central

    Kniazeva, Marina; Sieber, Matt; McCauley, Scott; Zhang, Kang; Watts, Jennifer L; Han, Min

    2003-01-01

    While the general steps of fatty acid (FA) biosynthesis are well understood, the individual enzymes involved in the elongation of long chain saturated and polyunsaturated FA (PUFA) are largely unknown. Recent research indicates that these enzymes might be of considerable physiological importance for human health. We use Caenorhabditis elegans to study FA elongation activities and associated abnormal phenotypes. In this article we report that the predicted C. elegans F11E6.5/ELO-2 is a functional enzyme with the FA elongation activity. It is responsible for the elongation of palmitic acid and is involved in PUFA biosynthesis. RNAi-mediated suppression of ELO-2 causes an accumulation of palmitate and an associated decrease in the PUFA fraction in triacylglycerides and phospholipid classes. This imbalance in the FA composition results in multiple phenotypic defects such as slow growth, small body size, reproductive defects, and changes in rhythmic behavior. ELO-2 cooperates with the previously reported ELO-1 in 20-carbon PUFA production, and at least one of the enzymes must function to provide normal growth and development in C. elegans. The presented data indicate that suppression of a single enzyme of the FA elongation machinery is enough to affect various organs and systems in worms. This effect resembles syndromic disorders in humans. PMID:12586704

  14. The Saccharomyces cerevisiae poly(A)-binding protein is subject to multiple post-translational modifications, including the methylation of glutamic acid.

    PubMed

    Low, Jason K K; Hart-Smith, Gene; Erce, Melissa A; Wilkins, Marc R

    2014-01-10

    Poly(A)-binding protein in mouse and man was recently found to be highly post-translationally modified. Here we analysed an ortholog of this protein, Pab1 from Saccharomyces cerevisiae, to assess the conservation and thus likely importance of these modifications. Pab1 showed the presence of six sites of methylated glutamate, five sites of lysine acetylation, and one phosphorylation of serine. Many modifications on Pab1 showed either complete conservation with those on human or mouse PABPC1, were present on nearby residues and/or were present in the same domain(s). The conservation of methylated glutamate, an unusual modification, was of particular note and suggests a conserved function. Comparison of methylated glutamate sites in human, mouse and yeast poly(A)-binding protein, along with methylation sites catalysed by CheR L-glutamyl protein methyltransferase from Salmonella typhimurium, revealed that the methylation of glutamate preferentially occurs in EE and DE motifs or other small regions of acidic amino acids. The conservation of methylated glutamate in the same protein between mouse, man and yeast suggests the presence of a eukaryotic l-glutamyl protein methyltransferase and that the modification is of functional significance.

  15. Estimates of subprimal yields from beef carcasses as affected by USDA grades, subcutaneous fat trim level, and carcass sex class and type.

    PubMed

    Griffin, D B; Savell, J W; Morgan, J B; Garrett, R P; Cross, H R

    1992-08-01

    One hundred beef carcasses were selected to represent the mix of cattle slaughtered across the United States. Selection criteria included breed type (60% British/continental European, 20% Bos indicus, and 20% dairy carcasses), sex class (beef and Bos indicus: 67% steers, 33% heifers; dairy: 100% steers), USDA quality grade (4% Prime, 53% Choice, and 43% Select), USDA yield grade (10% YG 1, 43% YG 2, 40% YG 3, and 7% YG 4), and carcass weight (steers: 272.2 to 385.6 kg, heifers: 226.8 to 340.2 kg). One side of each carcass was fabricated into boneless subprimals and minor cuts following Institutional Meat Purchase Specifications. After fabrication, subprimals were trimmed progressively of fat in .64-cm increments beginning with a maximum of 2.54 cm and ending with .64 cm. Linear regression models were developed for each individual cut, including fabrication byproduct items (bone, fat trim) to estimate the percentage yield of those cuts reported by USDA Market News. Strip loin, top sirloin butt, and gooseneck rounds from heifers tended to have a higher percentage yield at the same USDA yield grade than the same cuts from steers, possibly resulting from increased fat deposition on heifers. Percentage of fat trimmed from dairy steers was 2 to 3% lower than that from other sex-class/carcass types; however, due to increased percentage of bone and less muscle, dairy steers were lower-yielding. Fat trimmed from carcasses ranged from 7.9 to 15.6% as the maximum trim level decreased from 2.54 to .64 cm.

  16. Validation of the Soil Moisture Active Passive mission using USDA-ARS experimental watersheds

    NASA Astrophysics Data System (ADS)

    Cosh, M. H.; Jackson, T. J.; Bindlish, R.; Colliander, A.; Kim, S.; Das, N. N.; Yueh, S. H.; Bosch, D. D.; Goodrich, D. C.; Prueger, J. H.; Starks, P. J.; Livingston, S.; Seyfried, M. S.; Coopersmith, E. J.

    2015-12-01

    The calibration and validation program of the Soil Moisture Active Passive mission (SMAP) relies upon an international cooperative of in situ networks to provide ground truth references across a variety of landscapes. The USDA Agricultural Research Service operates several experimental watersheds which contribute to the validation of SMAP soil moisture products. These watersheds consist of a network of in situ sensors that measure soil moisture at a variety of depths including the 5 cm depth, which is critical for satellite validation. Comparisons of the in situ network estimates to the satellite products are ongoing, but initial results have shown strong correlation between satellite estimates and in situ soil moisture measurements once scaling functions were applied. The scaling methodologies for the in situ networks are being reviewed and evaluated. Results from the Little Washita, Fort Cobb, St. Joseph's and Little River Experimental Watersheds show good agreement between the satellite products and in situ measurements. Walnut Gulch results show high accuracy, although with the caveat that these domains are semi-arid with a substantially lower dynamic range. The South Fork Watershed is examined more closely for its detailed scaling function development as well as an apparent bias between satellite and in situ values.

  17. USDA Regional Climate Hubs - Partnering to bring information and tools to managers of working lands

    NASA Astrophysics Data System (ADS)

    Johnson, R.

    2014-12-01

    In February 2014, USDA announced the location of seven Regional Hubs for Risk Adaptation and Mitigation to Climate Change (Climate Hubs) and three "Sub Hubs". The mission of these Climate Hubs is to develop and deliver science-based region-specific information and technologies to agricultural and natural resource managers that enable climate-smart decision-making and to direct land managers to USDA programs that can assist them in implementing those decisions. This mission is similar to that of Cooperative Extension and the Agricultural Experiment Stations (both of which benefit from USDA funding); therefore it is crucial that we partner with Land Grant Universities in order to achieve this mission. As USDA stands up these Climate Hubs we are working closely with USDA agencies, Land Grant Universities, other federal climate science programs, and other partners to determine how best to provide usable information and tools to farmers, ranchers and forest land managers to enable them to make climate-smart decisions.

  18. School Meal Programs: More Systematic Development of Specifications Could Improve the Safety of Foods Purchased through USDA's Commodity Program. Report to the Ranking Member, Committee on Education and the Workforce, House of Representatives. GAO-11-376

    ERIC Educational Resources Information Center

    Shames, Lisa

    2011-01-01

    Through its commodity program, the U.S. Department of Agriculture (USDA) provides commodity foods at no cost to schools taking part in the national school meals programs. Commodities include raw ground beef, cheese, poultry, and fresh produce. Like federal food safety agencies, the commodity program has taken steps designed to reduce microbial…

  19. UPDATE ON US NATIONAL PRRS PROJECT PLANS: THE USDA FUNDED PRRS CAP PROGRAM, THE NPB FUNDED PRRS HOST GENETIC CONSORTIUM, AND US NATIONAL SWINE RESPIRATORY DISEASE NC229 PROJECT

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There are several major US efforts to address swine respiratory diseases. These include the US Department of Agriculture (USDA) funded PRRS coordinated agricultural project (PRRS CAP), the US National Pork Board (NPB) funded PRRS Host Genetic Consortium (PHGC), and the US national Swine Respiratory ...

  20. [Quality of data on folic acid content in vegetables included in several Spanish Food Composition Tables and new data on their folate content].

    PubMed

    Olivares, A B; Bernal, M J; Ros, G; Martínez, C; Periago, M J

    2006-01-01

    The relationship between adequate folate intake, adequate serum levels, and lowering the risk of suffering from cardiovascular diseases, neural tube defects, neural illness and some kind of cancers have been widely studied. Because of the expected health benefits, the consumption of foods with high folate content or enriched foods is increasing. Therefore, an adequate folate intake is important in order to reach acceptable serum levels. Reliable food composition data are necessary in order to evaluate and estimate the populations folate intake, elaborate diets and formulate recommended dietary intakes. For this reason, we revised folic acid data in Spanish Food Composition Tables (FCT). The quality of the data was evaluated and compared with other well-known international Food Composition Tables as well as with a high-resolution liquid chromatographic method (HPLC) validated in our laboratory. We evaluated all data about folate content, as well as all the information given like data origin, analytical method, sampling or original database. For the HPLC method, the food samples were incubated with hog kidney conjugase. After that, the samples were purified and concentrated by strong anion exchange (SAX), then the folate content was quantified by HPLC with a combination of two ultraviolet and fluorescence detectors. The evaluation and comparison of data was established according to some parameters, which define the quality of data, giving punctuation depending on the compliance with these parameters. The study of different sources showed that nutrients were different in definition, analysis method, units and expression of data, and that this fact could have a potential influence on TCA data values. In addition, it has been possible to show a wide variation in food number, name of these foods as well as the analysis of raw or cooked products with different composition. When the quality conditions were tested, the Spanish FCT had the lowest punctuation in folate

  1. Molecular mechanism for H2 release from BH3NH3, including the catalytic role of the Lewis acid BH3.

    PubMed

    Nguyen, Minh Tho; Nguyen, Vinh Son; Matus, Myrna H; Gopakumar, G; Dixon, David A

    2007-02-01

    Electronic structure calculations using various methods, up to the coupled-cluster CCSD(T) level, in conjunction with the aug-cc-pVnZ basis sets with n = D, T, and Q, extrapolated to the complete basis set limit, show that the borane molecule (BH3) can act as an efficient bifunctional acid-base catalyst in the H2 elimination reactions of XHnYHn systems (X, Y = C, B, N). Such a catalyst is needed as the generation of H2 from isoelectronic ethane and borane amine compounds proceeds with an energy barrier much higher than that of the X-Y bond energy. The asymptotic energy barrier for H2 release is reduced from 36.4 kcal/mol in BH3NH3 to 6.0 kcal/mol with the presence of BH3 relative to the molecular asymptote. The NH3 molecule can also participate in a similar catalytic process but induces a smaller reduction of the energy barrier. The kinetics of these processes was analyzed by both transition-state and RRKM theory. The catalytic effect of BH3 has also been probed by an analysis of the electronic densities of the transition structures using the atom-in-molecule (AIM) and electron localization function (ELF) approaches.

  2. How the CATCH eat smart program helps implement the USDA regulations in school cafeterias.

    PubMed

    Hoelscher, Deanna M; Mitchell, Paul; Dwyer, Johanna; Elder, John; Clesi, Ann; Snyder, Patricia

    2003-08-01

    This article describes the implementation of the U.S. Department of Agriculture's National School Lunch Program (NSLP) standards in school lunch menus in 56 intervention and 20 control schools from the Child and Adolescent Trial for Cardiovascular Health (CATCH) 5 years after the main trial, compared with 12 schools previously unexposed to CATCH. School food service personnel completed questionnaires to assess CATCH guideline implementation, demographic data, behavioral constructs, training, program material use, and participation in competing programs. Five days of menus and recipes were collected from school cafeteria staff, averaged, and compared to USDA School Meal Initiative (SMI) standards. Significant differences between intervention and unexposed schools were found for training and knowledge of CATCH and in mean percentage energy from fat and carbohydrates. Intervention schools most closely met USDA SMI recommendations for fat. Thus, the CATCH Eat Smart Program assisted school cafeterias in meeting USDA guidelines 5 years postimplementation. PMID:12929895

  3. DOE/USDA joint project to design and manufacture prototype equipment

    SciTech Connect

    Fuller, J.G.

    1996-07-01

    Design, assembly, prove-in, and performance testing of prototype equipment for the United States Department of Agriculture`s Subtropical Agriculture Research Laboratory (SARL) were completed by the Department of Energy`s Kansas City Plant. The plant is operated by AlliedSignal Federal Manufacturing & Technologies (FM&T). The prototype equipment was developed as part of the USDA`s efforts to mass produce parasitic wasps for organic control of boll weevils in cotton crops. This development was part of the Production Capability Assurance Program and also part of the Work For Others program. Design and assembly of this prototype equipment led to some new FM&T processes and technologies and exercised many others as described in the text and, at the same time, met the needs of the USDA.

  4. [Evaluation of ten fish species to be included as part of renal diet, due to their protein, phosphorus and fatty acids content].

    PubMed

    Castro-González, Maria Isabel; Maafs-Rodríguez, Ana Gabriela; Pérez-Gil Romo, Fernando

    2012-06-01

    Because renal disease is highly complex, its nutritional treatment is complicated and many foods are restricted, including fish because its phosphorus content. The aim of the present study was to analyze ten fillet fish species, commonly consumed in Mexico (Cyprinus carpio carpio, Ophichthus rex, Symphurus elongatus, Eucinostomus entomelas, Chirostoma patzcuaro, Bairdiella chrysoura, Salmo salar Oreochromis urolepis hornorum, Sphyraena guachancho, Istiophorus albicans), to determine their phosphorus (P), protein (Pr), cholesterol, sodium, potassium, vitamins D3 and E, and n-3 PUFA (EPA+DHA) according to the AOAC techniques, in order to identify which species could be included in renal diet; particularly because of their risk:benefit relations (calculated with those results). Protein values ranged from 16.5 to 33.5g/100 g of fillet; the specie with the highest phosphorus contest was Salmo salar, and with the lowest, Symphurus elongatus. EPA+DHA quantity ranged from 79.64 mg/100 g to 1,381.53 mg/100 g. Considering de P/Pr relation recommended to renal patients, all analyzed species (except Salmo salar, Ophichthus rex and Istiophorus albicans) could be included in their diet. As for the P/EPA+DHA relation, the species most recommended to renal patients are Symphurus elongatus, Bairdiella chrysoura and Sphyraena guachancho. PMID:23610899

  5. Consumer assessment of beef tenderloin steaks from various USDA quality grades at 3 degrees of doneness.

    PubMed

    O'Quinn, Travis G; Brooks, J Chance; Miller, Markus F

    2015-02-01

    A consumer study was conducted to determine palatability ratings of beef tenderloin steaks from USDA Choice, USDA Select, and USDA Select with marbling scores from Slight 50 to 100 (USDA High Select) cooked to various degrees of doneness. Steaks were randomly assigned to 1 of 3 degree of doneness categories: very-rare, medium-rare, or well-done. Consumers (N = 315) were screened for preference of degree of doneness and fed 4 samples of their preferred doneness (a warm-up and one from each USDA quality grade treatment in a random order). Consumers evaluated steaks on an 8-point verbally anchored hedonic scale for tenderness, juiciness, flavor, and overall like as well as rated steaks as acceptable or unacceptable for all palatability traits. Quality grade had no effect (P > 0.05) on consumer ratings for tenderness, juiciness, flavor, and overall like scores, with all traits averaging above a 7 ("like very much") on the 8-point scale. In addition, no differences (P > 0.05) were found in the percentage of samples rated as acceptable for all palatability traits, with more than 94% of samples rated acceptable for each trait in all quality grades evaluated. Steaks cooked to well-done had lower (P < 0.05) juiciness scores than steaks cooked to very-rare or medium-rare and were rated lower for tenderness (P < 0.05) than steaks cooked to a very-rare degree of doneness. Results indicate consumers were not able to detect differences in tenderness, juiciness, flavor, or overall like among beef tenderloin steaks from USDA Choice and Select quality grades.

  6. Long-Term Network Experiments and Interdisciplinary Campaigns Conducted by the USDA-Agricultural Research Service

    NASA Astrophysics Data System (ADS)

    Goodrich, D. C.; Kustas, W. P.; Cosh, M. H.; Moran, S. M.; Marks, D. G.; Jackson, T. J.; Bosch, D. D.; Rango, A.; Seyfried, M. S.; Scott, R. L.; Prueger, J. H.; Starks, P. J.; Walbridge, M. R.

    2014-12-01

    The USDA-Agricultural Research Service has led, or been integrally involved in, a myriad of interdisciplinary field campaigns in a wide range of locations both nationally and internationally. Many of the shorter campaigns were anchored over the existing national network of ARS Experimental Watersheds and Rangelands. These long-term outdoor laboratories provided a critical knowledge base for designing the campaigns as well as historical data, hydrologic and meteorological infrastructure coupled with shop, laboratory, and visiting scientist facilities. This strong outdoor laboratory base enabled cost-efficient campaigns informed by historical context, local knowledge, and detailed existing watershed characterization. These long-term experimental facilities have also enabled much longer term lower intensity experiments, observing and building an understanding of both seasonal and inter-annual biosphere-hydrosphere-atmosphere interactions across a wide range of conditions. A sampling of these experiments include MONSOON'90, SGP97, SGP99, Washita'92, Washita'94, SMEX02-05 and JORNEX series of experiments, SALSA, CLASIC and longer-term efforts over the ARS Little Washita, Walnut Gulch, Little River, Reynolds Creek, and OPE3 Experimental Watersheds. This presentation will review some of the highlights and key findings of these campaigns and long-term efforts including the inclusion of many of the experimental watersheds and ranges in the Long-Term Agro-ecosystems Research (LTAR) network. The LTAR network also contains several locations that are also part of other observational networks including the CZO, LTER, and NEON networks. Lessons learned will also be provided for scientists initiating their participation in large-scale, multi-site interdisciplinary science.

  7. Inframolecular acid base studies of the tris and tetrakis myo-inositol phosphates including the 1,2,3-trisphosphate motif

    NASA Astrophysics Data System (ADS)

    Dozol, Hélène; Blum-Held, Corinne; Guédat, Philippe; Maechling, Clarisse; Lanners, Steve; Schlewer, Gilbert; Spiess, Bernard

    2002-12-01

    The intrinsic acid-base properties of the phosphate groups of three myo-inositol derivatives which display the 1,2,3-trisphosphate motif, i.e. (±)- myo-inositol 1,2,3-trisphosphate (Ins(1,2,3)P 3), (±)- myo-inositol 1,2,3,6-tetrakisphosphate (Ins(1,2,3,6)P 4), and (±)- myo-inositol 1,2,3,5-tetrakisphosphate (Ins(1,2,3,5)P 4) are reported. The studies were performed in 0.2 M KCl solution at 37 °C, near physiological ionic strength and temperature. In addition, in order to shed light on the transition metal complexation properties of Ins(1,2,3)P 3, the influence of the Zn 2+ cations on its 31P NMR titration curves was investigated. From the titration curves as well as from the determined protonation microconstants, it appears that for Ins(1,2,3)P 3, the two lateral P1 and P3 phosphates strongly contribute to stabilise a proton on the central P2 phosphate. However, in the fully deprotonated form of Ins(1,2,3)P 3, P1 and P3 repulse each other so that they establish hydrogen bonds with, respectively, their neighbouring OH6 and OH4 hydroxyls. The 1,2,3-trisphosphate motif of Ins(1,2,3,5)P 4 behaves very similarly to that of Ins(1,2,3)P 3 indicating a poor interaction with the distant P5 phosphate. By contrast, moving a phosphate group from position 5 to position 6 on the myo-inositol ring as in Ins(1,2,3,6)P 4, leads to major changes in the basicity and cooperativity of the phosphate groups. Finally, the presence of Zn 2+ cations has a marked influence on the 31P NMR titration curves of Ins(1,2,3)P 3, leading to the conclusion that two equatorial phosphates, assisted by a middle axial one, afford an optimal chelating moiety that is able to occupy all sites of the metal coordination polyhedron which could be the reason for its antioxidant properties.

  8. Final master work plan : environmental investigations at former CCC/USDA facilities in Kansas, 2002 revision.

    SciTech Connect

    Burton, J. C.; Environmental Research

    2003-01-23

    The Commodity Credit Corporation (CCC) of the U.S. Department of Agriculture (USDA) has entered into an interagency agreement with the U.S. Department of Energy (DOE) under which Argonne National Laboratory provides technical assistance for hazardous waste site characterization and remediation for the CCC/USDA. Carbon tetrachloride is the contaminant of primary concern at sites in Kansas where former CCC/USDA grain storage facilities were located. Argonne applies its QuickSite(reg sign) Expedited Site Characterization (ESC) approach to these former facilities. The QuickSite environmental site characterization methodology is Argonne's proprietary implementation of the ESC process (ASTM 1998). Argonne has used this approach at several former CCC/USDA facilities in Kansas, including Agenda, Agra, Everest, and Frankfort. The Argonne ESC approach revolves around a multidisciplinary, team-oriented approach to problem solving. The basic features and steps of the QuickSite methodology are as follows: (1) A team of scientists with diverse expertise and strong field experience is required to make the process work. The Argonne team is composed of geologists, geochemists, geophysicists, hydrogeologists, chemists, biologists, engineers, computer scientists, health and safety personnel, and regulatory staff, as well as technical support staff. Most of the staff scientists are at the Ph.D. level; each has on average, more than 15 years of experience. The technical team works together throughout the process. In other words, the team that plans the program also implements the program in the field and writes the reports. More experienced scientists do not remain in the office while individuals with lesser degrees or experience carry out the field work. (2) The technical team reviews, evaluates, and interprets existing data for the site and the contaminants there to determine which data sets are technically valid and can be used in initially designing the field program. A basic

  9. Technical justification for a request to reclassify the former CCC/USDA facility at Canada, Kansas.

    SciTech Connect

    LaFreniere, L. M.; Environmental Science Division

    2007-12-21

    Contamination in groundwater at Canada, Kansas, was discovered in 1997, during limited private well sampling near former grain storage facilities of the Commodity Credit Corporation, U.S. Department of Agriculture (CCC/USDA). Subsequent investigations by the Kansas Department of Health and Environment (KDHE) confirmed carbon tetrachloride and nitrate concentrations in groundwater above the respective maximum contaminant levels (MCLs) of 5.0 {micro}g/L and 10.0 mg/L. The KDHE investigations identified both the former CCC/USDA grain storage facility and a private grain storage facility as likely sources for the carbon tetrachloride contamination. The CCC/USDA funded extension of a rural water district line to provide a permanent alternate water supply, and the KDHE has conducted long-term monitoring under the State Water Plan. This document presents an analysis of the available information for the Canada site, acquired in previous investigations and the long-term KDHE monitoring. This analysis forms the technical justification for a request to reclassify the former CCC/USDA grain storage facility at Canada as a site requiring no further action under the Intergovernmental Agreement (IGA) between the KDHE and the USDA's Farm Service Agency. The KDHE's long-term water level monitoring results indicate a consistent groundwater flow direction to the east-southeast. Consequently, the wells with the highest overall concentrations of carbon tetrachloride are downgradient from the private grain storage facility but not downgradient from the former CCC/USDA facility. The KDHE criterion for reclassification of a site is that contamination there should not pose an unacceptable risk, on the basis of analytical results for four consecutive, equally timed, sequenced sampling episodes over a period of no less than two years. In seven KDHE sampling events over a period of six years (2001-2007), the concentrations of carbon tetrachloride in the monitoring well on the former CCC/USDA

  10. Process of formulating USDA's Expanded Flavonoid Database for the Assessment of Dietary intakes: a new tool for epidemiological research.

    PubMed

    Bhagwat, Seema A; Haytowitz, David B; Wasswa-Kintu, Shirley I; Pehrsson, Pamela R

    2015-08-14

    The scientific community continues to be interested in potential links between flavonoid intakes and beneficial health effects associated with certain chronic diseases such as CVD, some cancers and type 2 diabetes. Three separate flavonoid databases (Flavonoids, Isoflavones and Proanthocyanidins) developed by the USDA Agricultural Research Service since 1999 with frequent updates have been used to estimate dietary flavonoid intakes, and investigate their health effects. However, each of these databases contains only a limited number of foods. The USDA has constructed a new Expanded Flavonoids Database for approximately 2900 commonly consumed foods, using analytical values from their existing flavonoid databases (Flavonoid Release 3.1 and Isoflavone Release 2.0) as the foundation to calculate values for all the twenty-nine flavonoid compounds included in these two databases. Thus, the new database provides full flavonoid profiles for twenty-nine predominant dietary flavonoid compounds for every food in the database. Original analytical values in Flavonoid Release 3.1 and Isoflavone Release 2.0 for corresponding foods were retained in the newly constructed database. Proanthocyanidins are not included in the expanded database. The process of formulating the new database includes various calculation techniques. This article describes the process of populating values for the twenty-nine flavonoid compounds for every food in the dataset, along with challenges encountered and resolutions suggested. The new expanded flavonoid database released on the Nutrient Data Laboratory's website would provide uniformity in estimations of flavonoid content in foods and will be a valuable tool for epidemiological studies to assess dietary intakes.

  11. Prediction of individual milk proteins including free amino acids in bovine milk using mid-infrared spectroscopy and their correlations with milk processing characteristics.

    PubMed

    McDermott, A; Visentin, G; De Marchi, M; Berry, D P; Fenelon, M A; O'Connor, P M; Kenny, O A; McParland, S

    2016-04-01

    The aim of this study was to evaluate the effectiveness of mid-infrared spectroscopy in predicting milk protein and free amino acid (FAA) composition in bovine milk. Milk samples were collected from 7 Irish research herds and represented cows from a range of breeds, parities, and stages of lactation. Mid-infrared spectral data in the range of 900 to 5,000 cm(-1) were available for 730 milk samples; gold standard methods were used to quantify individual protein fractions and FAA of these samples with a view to predicting these gold standard protein fractions and FAA levels with available mid-infrared spectroscopy data. Separate prediction equations were developed for each trait using partial least squares regression; accuracy of prediction was assessed using both cross validation on a calibration data set (n=400 to 591 samples) and external validation on an independent data set (n=143 to 294 samples). The accuracy of prediction in external validation was the same irrespective of whether undertaken on the entire external validation data set or just within the Holstein-Friesian breed. The strongest coefficient of correlation obtained for protein fractions in external validation was 0.74, 0.69, and 0.67 for total casein, total β-lactoglobulin, and β-casein, respectively. Total proteins (i.e., total casein, total whey, and total lactoglobulin) were predicted with greater accuracy then their respective component traits; prediction accuracy using the infrared spectrum was superior to prediction using just milk protein concentration. Weak to moderate prediction accuracies were observed for FAA. The greatest coefficient of correlation in both cross validation and external validation was for Gly (0.75), indicating a moderate accuracy of prediction. Overall, the FAA prediction models overpredicted the gold standard values. Near-unity correlations existed between total casein and β-casein irrespective of whether the traits were based on the gold standard (0.92) or mid

  12. 76 FR 20305 - USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2011 Raw Sugar Tariff...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ...; ] DEPARTMENT OF AGRICULTURE Office of the Secretary USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2011 Raw Sugar Tariff-Rate Quota AGENCY: Office of the Secretary, USDA. ACTION: Notice. SUMMARY: The Secretary of Agriculture today announced a reassignment of surplus sugar under domestic...

  13. 75 FR 38764 - USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2010 Raw Sugar Tariff...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ...; ] DEPARTMENT OF AGRICULTURE Office of the Secretary USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2010 Raw Sugar Tariff-Rate Quota AGENCY: Office of the Secretary, USDA. ACTION: Notice. SUMMARY: The Secretary of Agriculture today announced a reassignment of surplus sugar under domestic...

  14. 75 FR 22095 - USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2010 Raw Sugar Tariff...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-27

    ...; ] DEPARTMENT OF AGRICULTURE Office of the Secretary USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2010 Raw Sugar Tariff-Rate Quota AGENCY: Office of the Secretary, USDA. ACTION: Notice. SUMMARY: The Secretary of Agriculture today announced a reassignment of surplus sugar under domestic...

  15. 7 CFR 170.11 - How are farmers and vendors selected for participation in the USDA Farmers Market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... USDA Farmers Market? USDA reviews all applications and selects participants based primarily on the type... products to be sold. The selection of the participants is conducted by the market management to ensure a balanced product mix of fruits, vegetables, herbs, value-added products, and baked goods....

  16. 2 CFR 417.755 - When will I know whether the USDA suspension is continued or terminated?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 2 Grants and Agreements 1 2012-01-01 2012-01-01 false When will I know whether the USDA suspension is continued or terminated? 417.755 Section 417.755 Grants and Agreements Federal Agency Regulations... § 417.755 When will I know whether the USDA suspension is continued or terminated? The...

  17. 2 CFR 417.755 - When will I know whether the USDA suspension is continued or terminated?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 2 Grants and Agreements 1 2013-01-01 2013-01-01 false When will I know whether the USDA suspension is continued or terminated? 417.755 Section 417.755 Grants and Agreements Federal Agency Regulations... § 417.755 When will I know whether the USDA suspension is continued or terminated? The...

  18. 2 CFR 417.755 - When will I know whether the USDA suspension is continued or terminated?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false When will I know whether the USDA suspension is continued or terminated? 417.755 Section 417.755 Grants and Agreements Federal Agency Regulations... § 417.755 When will I know whether the USDA suspension is continued or terminated? The...

  19. 2 CFR 417.755 - When will I know whether the USDA suspension is continued or terminated?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 2 Grants and Agreements 1 2011-01-01 2011-01-01 false When will I know whether the USDA suspension is continued or terminated? 417.755 Section 417.755 Grants and Agreements Federal Agency Regulations... § 417.755 When will I know whether the USDA suspension is continued or terminated? The...

  20. Prioritization of Malus accessions for collection cryopreservation at the USDA-ARS National Center for Genetic Resources Preservation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-ARS National Plant Germplasm System maintains a grafted collection of apple accessions representing 49 taxa in Geneva, NY. Dormant buds of many of these accessions have been routinely cryopreserved at the USDA-ARS National Center for Genetic Resources Preservation (NCGRP) in Fort Collins, C...

  1. The effects of ursodeoxycholic acid treatment for intrahepatic cholestasis of pregnancy on maternal and fetal outcomes: a meta-analysis including non-randomized studies.

    PubMed

    Grand'Maison, Sophie; Durand, Madeleine; Mahone, Michèle

    2014-07-01

    Objectif : Les avantages de l’utilisation d’acide ursodésoxycholique (AUDC) pour la prise en charge de la cholestase intrahépatique de la grossesse (CIG) demeurent incertains. Une analyse Cochrane de 2010 ayant porté sur des essais comparatifs randomisés n’a pas été en mesure de se prononcer pour ou contre l’utilisation d’AUDC pour la prise en charge de la CIG. Nous avons mené une méta-analyse de la littérature, en englobant tant les études non randomisées (ENR) que les ECR. Nous avions pour objectif de déterminer si les patientes ayant participé aux ENR étaient comparables à celles qui avaient participé aux ECR; nous avions également pour objectif de déterminer si l’inclusion des ENR pouvait renforcer les données probantes disponibles et orienter la pratique clinique quant à l’utilisation d’AUDC chez les femmes qui présentent une CIG. Sources de données : Nous avons mené des recherches dans Medline (Ovid), Embase (Ovid), EMB Reviews, Cinahl (Ebsco) et Web of Knowledge (Thomson Reuters) en vue d’en tirer les articles publiés entre 1966 et juin 2012. Sélection des études : Nous avons inclus tous les ECR admissibles ayant comparé l’AUDC à un placebo ou à d’autres traitements et toutes les ENR ayant comparé l’AUDC à tout autre traitement chez des femmes présentant une CIG. Synthèse des données : Nous avons inclus 11 ECR (n = 625 grossesses) et six ENR (n = 211 grossesses). Bien que les femmes ayant participé aux ECR et aux ENR aient été comparables, la qualité des études était plus faible dans le cas des ENR. De façon générale, les femmes traitées à l’AUDC ont connu une atténuation du prurit dans 73 % des ECR et dans 100 % des ENR disposant de données disponibles. Les épreuves de fonction hépatique ont présenté une amélioration dans 82 % des ECR et dans 100 % des ENR disposant de données disponibles. Bien que l’utilisation d’AUDC n’ait pas affecté le taux de c

  2. Geochemical investigations and interim recommendations for priority abandoned mine sites on U.S.D.A. Forest Service lands, Mineral Creek watershed, San Juan County, Colorado

    USGS Publications Warehouse

    Nash, J.T.

    1999-01-01

    Field observations, sampling of mine dumps and mine drainage waters, and laboratory studies of dump materials have been made at mining areas deemed to be on public lands administered by the USDA Forest Service in the Mineral Creek watershed. Results of chemical analyses of dump materials, leachates of those materials, and of surface waters draining mines or dumps provide indications of where acid is generated or consumed, and what metals are mobilized below mines or dumps. Information on 25 sites is reviewed and reclamation priorities are ranked into four classes (high, medium, low priority, or no work required). The western side of the upper Animas watershed (the Mineral Creek watershed) has a history of mining and prospecting for about 130 years. The intensity of miningrelated disturbance is higher than in most parts of the San Juan Mountains region, but actually is much less than the eastern half of the watershed (US BLM lands) and none of the mines moved millions of tons of rock and ore as in some of the eastern mines. The majority of the roughly one thousand mining sites on the USFS lands are very small (less than 100 tons or 70 cubic yards of dump material), are more than 2 miles from a major stream, or are so inaccessible as to prohibit reclamation. Twenty five sites have been considered by others to have significant size and potential for significant environmental degradation. These most significant mining areas were evaluated by multiple criteria, including tendency to generate acid or liberate toxic metals, observed acidic pH or dead vegetation (?kill zones?) below dumps or adits, potential mobility of metals, and likelihood of transport into streams of the watershed. In the author?s opinion, no single measurable parameter, such as metal concentration, is reliable for ranking significance or feasibility of reclamation. Rather, subjective estimates are required to evaluate combinations of, or interactions among, several parameters. The most subjective

  3. Beet curly top resistance in germplasm from the USDA-ARS Ft. Collins program, 2014

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ninety-seven sugar beet (Beta vulgaris L.) germplasm lines from the USDA-ARS Ft. Collins sugar beet program, a resistant control germplasm (1996A008), and three commercial control cultivars [SV2012RR (susceptible), Monohikari (susceptible) and HM PM90 (resistant)] were screened for response to Beet ...

  4. Assessment of USDA-NRCS Rangeland Conservation Programs: Recommendation for an Evidence-based Conservation Platform

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Conservation Effects Assessment Project (CEAP) was created in response to a request from the Office of Management and Budget that the U.S. Department of Agriculture - Natural Resource Conservation Service (USDA – NRCS) document the societal benefits anticipated to accrue from a major increase in...

  5. Genetic diversity among wheat accessions from the USDA National Small Grains Collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Accessions of Triticum aestivum subsp. aestivum from the USDA-ARS National Small Grains Collection (NSGC) are a resource for wheat scientists worldwide. The genetic diversity of the wheat core subset, representing approximately 10% of the collection’s 42138 T. aestivum accessions, was examined using...

  6. 76 FR 36512 - USDA Increases the Domestic Sugar Overall Allotment Quantity, Reassigns Domestic Cane Sugar...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-22

    ... Office of the Secretary USDA Increases the Domestic Sugar Overall Allotment Quantity, Reassigns Domestic Cane Sugar Allotments, and Increases the Fiscal Year 2011 Raw Sugar Tariff-Rate Quota AGENCY: Office of... in the domestic sugar Overall Allotment Quantity (OAQ); a reassignment of surplus sugar...

  7. 76 FR 61074 - USDA Increases the Fiscal Year 2011 Tariff-Rate Quota for Refined Sugar

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-03

    ... Office of the Secretary USDA Increases the Fiscal Year 2011 Tariff-Rate Quota for Refined Sugar AGENCY... increase in the fiscal year (FY) 2011 refined sugar tariff-rate quota (TRQ) of 136,078 metric tons raw... MTRV for sugars, syrups, and molasses (collectively referred to as refined sugar) described...

  8. 7 CFR 170.6 - How are potential market participants identified for the USDA Farmers Market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...? Potential market participants are recruited by AMS market management through local farm organizations in the... 7 Agriculture 3 2010-01-01 2010-01-01 false How are potential market participants identified for the USDA Farmers Market? 170.6 Section 170.6 Agriculture Regulations of the Department of...

  9. Dietary intake of children participating in the USDA Summer Food Service Program

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA summer food service program (SFSP) provides free lunches during the summer. This study examined the foods selected and consumed by participating children. Three hundred and two children were observed in 14 schools during a 4-week period in June, 2011; 50% were male; 75% were in elementary s...

  10. World Saccharum Collection at the USDA Subtropical Research Station, National Germplasm Repository Miami, Florida

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In 1980, the U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS), established a genebank, the National Clonal Germplasm Repository (NCGR) in Miami, Florida. This repository is devoted to conservation of subtropical and tropical fruit, sugarcane and related grasses and ornamen...

  11. 17. Photocopy of Sheet 5 of Building Plan R41A, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Photocopy of Sheet 5 of Building Plan R4-1A, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) FRAMING PLAN, KITCHEN CABINET PLAN AND BATHTUB ARCH PLAN - Buffalo Guard Station, Residence, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  12. Research Update: The USDA-ARS-Conservation and Production Research Laboratory, Bushland, Texas

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This presentation/manuscript provide a brief summary of beef cattle feeding-related research conducted at the USDA-ARS-Conservation and Production Research Laboratory, Bushland, Texas, over the past four years. It summarizes data that has been published in scientific journals, in symposia and confer...

  13. Integrating Near Infrared Spectroscopy (NIR) into the USDA-ARS sugarcane breeding program in Houma, LA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Near Infrared Spectroscopy (NIRs) is a relatively new technique that has the potential to benefit Louisiana’s sugarcane industry, and is being successfully used in other parts of the world (e.g., South Africa) and even Florida. Recently, the USDA-ARS in Houma, LA purchased a NIR Cane Presentation Sy...

  14. 2 CFR 417.800 - What are the USDA causes for debarment?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 2 Grants and Agreements 1 2013-01-01 2013-01-01 false What are the USDA causes for debarment? 417... causes for debarment? A Federal agency may debar a person for— (a) Conviction of or civil judgment for... requirement applicable to a public agreement or transaction; (c) Any of the following causes: (1)...

  15. 2 CFR 417.800 - What are the USDA causes for debarment?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 2 Grants and Agreements 1 2012-01-01 2012-01-01 false What are the USDA causes for debarment? 417... causes for debarment? A Federal agency may debar a person for— (a) Conviction of or civil judgment for... requirement applicable to a public agreement or transaction; (c) Any of the following causes: (1)...

  16. The social sciences, philosophy, and the cultural turn in the 1930s USDA.

    PubMed

    Jewett, Andrew

    2013-01-01

    One of the more unusual attempts by the American state to mobilize academic expertise unfolded in the late 1930s, when the United States Department of Agriculture (USDA) hired scholars in the "culture and personality" fields and philosophy to aid its efforts to promote economic, social, and cultural change in the countryside. USDA progressives also reached out to disciplinary scholars in other ways as they sought to institute a deliberative mode of planning in local communities and to remake the curricula of the land-grant colleges in support of that project. These USDA initiatives and scholars' responses reveal that scientific knowledge was mobilized in the 1930s not just for the instrumental purpose of regulating economic behavior but also to explain and legitimate federal programs and to inform ambitious projects for cultural change. At the USDA, as at many other sites between the wars, scientific thinkers turned to the social sciences and philosophy in order to understand and then change the public mind.

  17. Adaptation to climate variability: The role of the USDA Southern Plains Climate Hub

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Southern Plains USDA Climate Hub was established in 2014 in El Reno, Oklahoma to develop and deliver science-based, information and technologies to agricultural and natural resource land managers that enable climate-informed decision-making, and to provide access to assistance to implement those...

  18. Evaluation of the USDA Northeast Area-wide Tick Control Project by Meta-analysis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    As part of the Northeast Area-wide Tick Control Project (NEATCP), meta-analyses were performed using pooled data on the extent of tick-vector control achieved through seven concurrent studies, conducted within five states, using USDA ‘4-Poster’ devices to deliver targeted-acaricide to white-tailed d...

  19. 7 CFR 658.7 - USDA assistance with Federal agencies' reviews of policies and procedures.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... policies and procedures. 658.7 Section 658.7 Agriculture Regulations of the Department of Agriculture... PROTECTION POLICY ACT § 658.7 USDA assistance with Federal agencies' reviews of policies and procedures. (a... current provisions of law, administrative rules and regulations, and policies and procedures applicable...

  20. 7 CFR 658.7 - USDA assistance with Federal agencies' reviews of policies and procedures.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... policies and procedures. 658.7 Section 658.7 Agriculture Regulations of the Department of Agriculture... PROTECTION POLICY ACT § 658.7 USDA assistance with Federal agencies' reviews of policies and procedures. (a... current provisions of law, administrative rules and regulations, and policies and procedures applicable...

  1. 7 CFR 1450.5 - Performance based on advice or action of USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false Performance based on advice or action of USDA. 1450.5 Section 1450.5 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOMASS CROP ASSISTANCE...

  2. 7 CFR 1450.5 - Performance based on advice or action of USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false Performance based on advice or action of USDA. 1450.5 Section 1450.5 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOMASS CROP ASSISTANCE...

  3. 7 CFR 1450.5 - Performance based on advice or action of USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false Performance based on advice or action of USDA. 1450.5 Section 1450.5 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOMASS CROP ASSISTANCE...

  4. 7 CFR 1450.5 - Performance based on advice or action of USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false Performance based on advice or action of USDA. 1450.5 Section 1450.5 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOMASS CROP ASSISTANCE...

  5. School Nutrition Directors' Perspectives on Preparing for and Implementing USDA's New School Meal Regulations

    ERIC Educational Resources Information Center

    Yon, Bethany A.; Amin, Sarah A.; Taylor, Jennifer C.; Johnson, Rachel K.

    2016-01-01

    Purpose/Objectives: The U.S. Department of Agriculture's (USDA) new school meals regulations went into effect in July 2012. The purpose of this research was to explore school nutrition director's (SNDs) perspectives and attitudes about the new regulations and to identify strategies used to prepare for and subsequently implement the regulations.…

  6. 75 FR 3892 - USDA Section 538 Guaranteed Rural Rental Housing Program; 2010 Industry Forums-Open...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-25

    ...--Open Teleconference and/or Web Conference Meetings AGENCY: Rural Housing Service, USDA. ACTION: Notice. SUMMARY: This notice announces a series of teleconference and/or Web conference meetings regarding the... in the teleconference and/or web conference meetings. DATES: The dates and times for...

  7. The USDA-ARS experimental watershed network-Evolution, lessons learned and moving forward

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-Agricultural Research Service’s Experimental Watershed Network grew from Dust Bowl era efforts of the Soil Conservation Service in the mid 1930’s with the establishment of watersheds in three States; one of which is still in operation. In the mid-50’s five centers with intensively instrumen...

  8. Associating mapping of stigma characteristics using the USDA rice core collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A mini-core from the USDA rice core collection was phenotyped for nine traits of stigma and spikelet and genotyped with 109 DNA markers. Marker-trait association mapping was used to identify the regions associated with the nine traits. Resulting associations were adjusted using false discovery rate ...

  9. Musa spp. germplasm management: microsatellite fingerprinting of USDA-ARS National Plant Germplasm System (NPGS) collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-ARS Tropical Agriculture Research Station (TARS) is responsible for conserving germplasm of a number of important agricultural crop species. A banana (Musa spp.) collection has been established at TARS that is comprised of diploid, triploid and tetraploid accessions of cultivated, ornament...

  10. 77 FR 49774 - Notice of Request for Extension of a Currently Approved Information Collection; USDA Registration...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-17

    ... must be identity proofed, in addition to completing an electronic self registration. Identity proofing... verification of identity, authorization, and electronic signatures. USDA's eAuth service obtains customer information through an electronic self registration process provided through the eAuth Web site....

  11. Evaluating citrus germplasm for huanglongbing (HLB) resistance: USDA-ARS Inoculation Program

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Asian citrus psyllid (ACP), Diaphorina citri, is an important pest because it vectors bacteria responsible for a serious disease of citrus known as huanglongbing (citrus greening disease). USDA-ARS researchers recently established a program for screening citrus germplasm for resistance to the di...

  12. USDA food and nutrient databases provide the infrastructure for food and nutrition research, policy and practice

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA food and nutrient databases provide the basic infrastructure for food and nutrition research, nutrition monitoring, and dietary practice. They have had a long history that goes back to 1892, and are unique, as they are the only databases available in the public domain that perform these fu...

  13. 2 CFR 417.800 - What are the USDA causes for debarment?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Federal agency taken pursuant to 48 CFR part 9, subpart 9.4, before August 25, 1995; (2) Knowingly doing... 2 Grants and Agreements 1 2011-01-01 2011-01-01 false What are the USDA causes for debarment? 417... DEPARTMENT OF AGRICULTURE NONPROCUREMENT DEBARMENT AND SUSPENSION Debarment § 417.800 What are the...

  14. 11. Photocopy of Sheet 3 of Building Plan R451, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Photocopy of Sheet 3 of Building Plan R4-51, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) Construction Details and Floor Framing. - Buffalo Guard Station, Office, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  15. 12. Photocopy of Sheet 5 of Building Plan R453 (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Photocopy of Sheet 5 of Building Plan R4-53 (USDA, Forest Service Intermountain Region, Ogden. File 7300, 'Buildings'.) PLAN OF KITCHEN CABINETS AND CONSTRUCTION DETAILS - Buffalo Guard Station, Residence, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  16. 9. Photocopy of Sheet 1 of Building Plan R451, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Photocopy of Sheet 1 of Building Plan R4-51, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) Front, Rear, and Side Elevations and Floor Plan. - Buffalo Guard Station, Office, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  17. 16. Photocopy of Sheet 4 of Building Plan R41A, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Photocopy of Sheet 4 of Building Plan R4-1A, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) SIDE ELEVATION AND DINING ALCOVE PLAN - Buffalo Guard Station, Residence, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  18. 15. Photocopy of Sheet 1 of Building Plan R41A, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Photocopy of Sheet 1 of Building Plan R4-1A, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) FRONT ELEVATION AND FLOOR PLAN - Buffalo Guard Station, Residence, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  19. 11. Photocopy of Sheet 4 of Building Plan R453 (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Photocopy of Sheet 4 of Building Plan R4-53 (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) SIDE ELEVATION AND FRAMING - Buffalo Guard Station, Residence, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  20. 10. Photocopy of Sheet 2 of Building Plan R451, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Photocopy of Sheet 2 of Building Plan R4-51, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) Framing and Foundation Plans. - Buffalo Guard Station, Office, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  1. 2 CFR 417.800 - What are the USDA causes for debarment?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Federal agency taken pursuant to 48 CFR part 9, subpart 9.4, before August 25, 1995; (2) Knowingly doing... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false What are the USDA causes for debarment? 417... DEPARTMENT OF AGRICULTURE NONPROCUREMENT DEBARMENT AND SUSPENSION Debarment § 417.800 What are the...

  2. 3. Photocopy of Sheet 1 of Building Plan R495D, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopy of Sheet 1 of Building Plan R4-95D, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) ELEVATION, FRAMING AND CONSTRUCTION DETAILS - Buffalo Guard Station, Pumphouse, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  3. 5. Photocopy of Sheet 2 of Building Plan R421, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Photocopy of Sheet 2 of Building Plan R4-21, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings') BACK SIDE ELEVATIONS AND CONSTRUCTION DETAILS - Buffalo Guard Station, Garage, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  4. 7 CFR 4290.1940 - Integration of this part with other regulations applicable to USDA's programs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... National Appeals Division within the USDA of adverse decisions, as defined in 7 CFR 11.1, pursuant to 7 CFR... applicable to this part, the Secretary will comply with subpart V of 7 CFR part 3015, “Intergovernmental... to this part, the Secretary will comply with subpart B of 7 CFR part 1806. The Secretary has...

  5. 6. Photocopy of Sheet 3 of Building Plan R421, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Photocopy of Sheet 3 of Building Plan R4-21, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings') FRAMING PLAN AND GARAGE DOOR DETAIL - Buffalo Guard Station, Garage, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  6. 7 CFR 4290.504 - Equipment of USDA or SBA officials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false Equipment of USDA or SBA officials. 4290.504 Section 4290.504 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS... (âRBICâ) PROGRAM Managing the Operations of a RBIC General Requirements § 4290.504 Equipment of...

  7. 7 CFR 4290.1940 - Integration of this part with other regulations applicable to USDA's programs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... National Appeals Division within the USDA of adverse decisions, as defined in 7 CFR 11.1, pursuant to 7 CFR... applicable to this part, the Secretary will comply with subpart V of 7 CFR part 3015, “Intergovernmental... to this part, the Secretary will comply with subpart B of 7 CFR part 1806. The Secretary has...

  8. 10. Photocopy of Sheet 1 of Building Plan R453 (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Photocopy of Sheet 1 of Building Plan R4-53 (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings'.) FRONT AND SIDE ELEVATIONS AND FLOOR PLAN - Buffalo Guard Station, Residence, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  9. 4. Photocopy of Sheet 1 of Building Plan R421, (USDA, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Photocopy of Sheet 1 of Building Plan R4-21, (USDA, Forest Service, Intermountain Region, Ogden. File 7300, 'Buildings') FRONT AND SIDE ELEVATIONS, FLOOR AND FOUNDATION PLANS. - Buffalo Guard Station, Garage, U.S. Highway 20/191 at Buffalo River, Island Park, Fremont County, ID

  10. 7 CFR 4290.504 - Equipment of USDA or SBA officials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false Equipment of USDA or SBA officials. 4290.504 Section 4290.504 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS... (âRBICâ) PROGRAM Managing the Operations of a RBIC General Requirements § 4290.504 Equipment of...

  11. 7 CFR 4290.1940 - Integration of this part with other regulations applicable to USDA's programs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... National Appeals Division within the USDA of adverse decisions, as defined in 7 CFR 11.1, pursuant to 7 CFR... applicable to this part, the Secretary will comply with subpart V of 7 CFR part 3015, “Intergovernmental... to this part, the Secretary will comply with subpart B of 7 CFR part 1806. The Secretary has...

  12. 7 CFR 4290.1940 - Integration of this part with other regulations applicable to USDA's programs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... National Appeals Division within the USDA of adverse decisions, as defined in 7 CFR 11.1, pursuant to 7 CFR... applicable to this part, the Secretary will comply with subpart V of 7 CFR part 3015, “Intergovernmental... to this part, the Secretary will comply with subpart B of 7 CFR part 1806. The Secretary has...

  13. 7 CFR 12.11 - Action based upon advice or action of USDA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... make such benefits available to the extent that similar relief would be allowed under 7 CFR part 718. ... 7 Agriculture 1 2011-01-01 2011-01-01 false Action based upon advice or action of USDA. 12.11 Section 12.11 Agriculture Office of the Secretary of Agriculture HIGHLY ERODIBLE LAND AND...

  14. 7 CFR 12.11 - Action based upon advice or action of USDA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... make such benefits available to the extent that similar relief would be allowed under 7 CFR part 718. ... 7 Agriculture 1 2012-01-01 2012-01-01 false Action based upon advice or action of USDA. 12.11 Section 12.11 Agriculture Office of the Secretary of Agriculture HIGHLY ERODIBLE LAND AND...

  15. 7 CFR 12.11 - Action based upon advice or action of USDA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... make such benefits available to the extent that similar relief would be allowed under 7 CFR part 718. ... 7 Agriculture 1 2014-01-01 2014-01-01 false Action based upon advice or action of USDA. 12.11 Section 12.11 Agriculture Office of the Secretary of Agriculture HIGHLY ERODIBLE LAND AND...

  16. 7 CFR 12.11 - Action based upon advice or action of USDA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... make such benefits available to the extent that similar relief would be allowed under 7 CFR part 718. ... 7 Agriculture 1 2013-01-01 2013-01-01 false Action based upon advice or action of USDA. 12.11 Section 12.11 Agriculture Office of the Secretary of Agriculture HIGHLY ERODIBLE LAND AND...

  17. 7 CFR 12.11 - Action based upon advice or action of USDA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... make such benefits available to the extent that similar relief would be allowed under 7 CFR part 718. ... 7 Agriculture 1 2010-01-01 2010-01-01 false Action based upon advice or action of USDA. 12.11 Section 12.11 Agriculture Office of the Secretary of Agriculture HIGHLY ERODIBLE LAND AND...

  18. Formulation and Recipe Calculations in the USDA National Nutrient Databank System

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objectives of the presentation are to: 1) familiarize representatives of the Office of Pesticide Programs of the Environmental Protection Agency with the Nutrient Data Laboratory's USDA National Nutrient Database for Standard Reference and its relationship to the Food Surveys Research Group's Fo...

  19. USDA Estimates of the Cost of Raising a Child: A Guide to Their Use and Interpretation.

    ERIC Educational Resources Information Center

    Edwards, Carolyn S.

    This guide describes estimates of the cost of raising a child made by the Family Economics Research Group of the United States Department of Agriculture (USDA). The guide starts with a description of what estimates are available, giving short profiles of the cost of raising urban, rural nonfarm, and rural farm children. The next section defines…

  20. Brook trout nutritional analysis for inclusion into the USDA National Nutrient Database for Standard Reference

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Many species of wild game and fish that are legal to hunt or catch do not have nutrition information in the USDA National Nutrient Database for Standard Reference (SR). Among those species that lack nutrition information are brook trout. The research team worked with the Nutrient Data Laboratory wit...

  1. Model development and applications at the USDA-ARS National Soil Erosion Research Laboratory

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The United States Department of Agriculture (USDA) has a long history of development of soil erosion prediction technology, initially with empirical equations like the Universal Soil Loss Equation (USLE), and more recently with process-based models such as the Water Erosion Prediction Project (WEPP)...

  2. Beta PIs from the USDA-ARS NPGS evaluated for resistance to Cercospora beticola, 2015

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Thirty Plant Introductions (PIs) from the USDA-ARS National Plant Germplasm System were evaluated for resistance to Cercospora beticola in an artificially produced epiphytotic at the Saginaw Valley Research and Extension Center near Frankenmuth, MI. An additional seven entries resulting from crossi...

  3. Update on USDA advanced citrus scion selections for Indian River Citrus League

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In an effort to help maintain the Florida citrus industry, the USDA US Horticultural Research Laboratory (USHRL) citrus breeding team is working to make advanced selections available for limited testing earlier and more broadly than in the past to quickly identify material with commercial potential....

  4. Validation of the Soil Moisture Active Passive mission using USDA-ARS experimental watersheds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The calibration and validation program of the Soil Moisture Active Passive mission (SMAP) relies upon an international cooperative of in situ networks to provide ground truth references across a variety of landscapes. The USDA Agricultural Research Service operates several experimental watersheds wh...

  5. Roadmap of the USDA peanut germplasm collection: past, present and future direction

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA ARS PGRCU maintains the second largest Arachis germplasm collection in the world with 9,321 cultivated and 655 wild entries. In the last twenty years, USA germplasm has been provided to over 52 countries around the world for research and breeding purposes. This collection has proven to be...

  6. Cryopreservation of citrus in the USDA-ARS national plant germplasm system collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Diverse Citrus germplasm from the USDA-ARS National Plant Germplasm System, University of California Riverside, and the California citrus industry is at risk of being lost as a result of disease infestations and unexpected weather disasters. At the National Center for Genetic Resources Preservation ...

  7. Beet curly top resistance of USDA-ARS National Plant Germplasm System plant introductions, 2010

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Twenty-six wild beet (Beta vulgaris subsp. maritima (L.) Arcang) accessions from the Beta collection of the USDA-ARS National Plant Germplasm System were screened for resistance to Beet severe curly top virus (BSCTV) and other closely related Curtovirus species in 2010. The curly top evaluation was...

  8. History and Accomplishments of USDA-ARS Avian Disease and Oncology Laboratory

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-Agricultural Research Service Avian Disease and Oncology Laboratory (ADOL) in East Lansing, Michigan, formerly known as Regional Poultry Research Laboratory, was dedicated on August 8, 1939. Its establishment was the result of joint efforts by the Agricultural Experiment Stations of the Nor...

  9. USDA-ARS Ft. Collins germplasm screened for resistance to Beet curly top, 2015

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fifty sugar beet (Beta vulgaris L.) germplasm lines produced by the USDA-ARS Ft. Collins sugar beet program and two commercial check cultivars [SV2012RR (susceptible) and HM PM90 (resistant)] were screened for resistance to Beet curly top virus (BCTV). The curly top evaluation was conducted at the ...

  10. School Nutrition Professionals' Usage and Perceptions of USDA Recipes and the Impact of Student Enrollment

    ERIC Educational Resources Information Center

    Rushing, Keith; Johnson, J. T.

    2015-01-01

    Purpose/Objectives: The purpose of this study was to explore the frequency of usage of the United States Department of Agriculture (USDA) Recipes for Schools and investigate factors influencing their usage. The relationship between these variables and school district size (student enrollment) was also investigated. Methods: An expert panel…

  11. The USDA and K-12 Partnership: A Model Program for Federal Agencies

    ERIC Educational Resources Information Center

    Scott, Timothy P.; Wilson, Craig; Upchurch, Dan R.; Goldberg, Maria; Bentz, Adrienne

    2011-01-01

    The Future Scientists Program of Texas A&M University and the Agricultural Research Service branch of USDA serves as a model program of effective collaboration between a federal agency and K-12. It demonstrates true partnership that contextualizes learning of science and provides quality professional development, benefiting teachers and their…

  12. Mapping QTLs for improving grain yield using the USDA rice mini-core collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Yield is the most important and complex trait for genetic improvement in crops, and marker-assisted selection enhances the improvement efficiency. The USDA rice mini-core collection derived from over 18,000 accessions of global origins is an ideal panel for association mapping. We phenotyped 203 O. ...

  13. Estimating conservation needs for rangelands using USDA National Resources Inventory Assessments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-Natural Resources Conservation Service (NRCS) has used resource inventories for over 65 years to assess the Nation’s natural resources on non-Federal lands. Since 1995, an interagency group composed of the NRCS, Agricultural Research Service, and Geological Survey have worked together to de...

  14. USDA 846-1 fractal melon and derived recombinant inbred lines

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Agricultural Research Service, United States Department of Agriculture announces the release of a melon (Cucumis melo L.) breeding line with highly branched, fractal-type architectural growth habit and 81 derived recombinant inbred lines (RIL). The indeterminate, monoecious USDA 846-1 produces 2...

  15. The USDA Poultry Research Unit at Mississippi State: Impacting the Bottom Line of the Poultry Research.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-ARS-Poultry Research Unit at Mississippi State has been addressing environmental, nutritional and mycoplasmal research for the poultry industry since 1965. Originally, the South Central Poultry Research Laboratory (SCPRL), it has been, from its inception, staffed with individuals whose col...

  16. 7 CFR 170.4 - Who may participate in the USDA Farmers Market?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Section 170.4 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) MISCELLANEOUS MARKETING PRACTICES UNDER THE AGRICULTURAL MARKETING ACT OF 1946 USDA FARMERS MARKET § 170.4 Who...

  17. USDA-ARS Poisonous Plant Research Laboratory: History and current research on western North American rangelands

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Poisonous plants on western North American rangelands have historically been troublesome to livestock producers. Research on toxic plants was initiated by U.S. Department of Agriculture in the late 1890’s to solve problems for the livestock industry. The USDA-ARS Poisonous Plant Research Laborator...

  18. High-impact animal health research conducted at the USDA's National Animal Disease Center

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Commissioned by President Dwight Eisenhower in 1958 and opened with a dedication ceremony in December 1961, the USDA, Agricultural Research Service (ARS), National Animal Disease Center (NADC) celebrated its 50-year anniversary in November 2011. Over these 50 years, the NADC established itself amon...

  19. Protein and quality analyses of accessions from the USDA soybean germplasm collection for tofu production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Food-grade soybeans with large seed size, uniformity, clear hilum, and high 11S/7S ratio are favored by the food industry for making tofu. In order to search for soybean lines with desirable characteristics for making foods, twenty-two soybean lines were selected from the USDA-Soybean Germplasm Coll...

  20. Characterization of the USDA Rice World Genebank usng a core collection strategy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA Rice World Genebank contains more than 18,000 accessions originated from 116 countries displayed at www.ars-grin.gov with descriptive data for each. The resources and expertise required to fully characterize the collection is limited by the sheer number of accessions. Development of a Core ...

  1. Introduction to the USDA-Agricultural Research Service Poisonous Plant Research Laboratory Special Rangelands Issue

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-ARS Poisonous Plant Research Labortory (PPRL) in Logan, UT will sponsor an edition of the magazine Rangelands. This paper provides a brief history and overview of the PPRL, mission statement, research objectives by CRIS, and the disciplines involved in the research....

  2. Update to the USDA-ARS fixed-wing spray nozzle models

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The current USDA ARS Aerial Spray Nozzle Models were updated to reflect both new standardized measurement methods and systems, as well as, to increase operational spray pressure, aircraft airspeed and nozzle orientation angle limits. The new models were developed using both Central Composite Design...

  3. Association mapping of agronomic and quality traits in USDA pea single-plant collection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Association mapping is an efficient approach for the identification of the molecular basis of agronomic traits in crop plants. For this purpose in pea (Pisum sativum L.), we genotyped and phenotyped individual lines of the single-plant derived core collection of the USDA pea single-plant (PSP) colle...

  4. Tick-borne Diseases in Animals and USDA Research on Tick Control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Tick-borne diseases represent a major threat to animal health in the United States. The cattle industry in the United States has benefited greatly from the continued USDA efforts through the Cattle Fever Tick Eradication Program in preventing the re-introduction of cattle ticks and associated pathog...

  5. USDA food-borne virus research initiatives at Delaware State University

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA ARS Microbial Safety of Aquaculture Products Center of Excellence located on the campus of Delaware State University is conducting research geared toward; 1) improving detection methods for virus contamination of shellfish; 2) understanding how and why viruses persist within shellfish; and ...

  6. 7 CFR 3.45 - USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false USDA payment authorizing agency offset of pro rata... of pro rata share of payments due entity in which debtor participates. (a) A USDA payment authorizing..., may offset: (1) A debtor's pro rata share of USDA payments due any entity in which the...

  7. 41 CFR 102-75.1085 - When using a delegation of real property disposal authority under this subpart, is USDA required...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... of real property disposal authority under this subpart, is USDA required to report excess property to... DISPOSAL Delegations Delegation to the Department of Agriculture (usda) § 102-75.1085 When using a delegation of real property disposal authority under this subpart, is USDA required to report excess...

  8. 41 CFR 102-75.1085 - When using a delegation of real property disposal authority under this subpart, is USDA required...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... of real property disposal authority under this subpart, is USDA required to report excess property to... DISPOSAL Delegations Delegation to the Department of Agriculture (usda) § 102-75.1085 When using a delegation of real property disposal authority under this subpart, is USDA required to report excess...

  9. 7 CFR 3.45 - USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false USDA payment authorizing agency offset of pro rata... Secretary of Agriculture DEBT MANAGEMENT Administrative Offset § 3.45 USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor participates. (a) A USDA payment...

  10. 41 CFR 102-75.1085 - When using a delegation of real property disposal authority under this subpart, is USDA required...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of real property disposal authority under this subpart, is USDA required to report excess property to... DISPOSAL Delegations Delegation to the Department of Agriculture (usda) § 102-75.1085 When using a delegation of real property disposal authority under this subpart, is USDA required to report excess...

  11. 41 CFR 102-75.1085 - When using a delegation of real property disposal authority under this subpart, is USDA required...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of real property disposal authority under this subpart, is USDA required to report excess property to... DISPOSAL Delegations Delegation to the Department of Agriculture (usda) § 102-75.1085 When using a delegation of real property disposal authority under this subpart, is USDA required to report excess...

  12. 7 CFR 3.45 - USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false USDA payment authorizing agency offset of pro rata... Secretary of Agriculture DEBT MANAGEMENT Administrative Offset § 3.45 USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor participates. (a) A USDA payment...

  13. 41 CFR 102-75.1085 - When using a delegation of real property disposal authority under this subpart, is USDA required...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... of real property disposal authority under this subpart, is USDA required to report excess property to... DISPOSAL Delegations Delegation to the Department of Agriculture (usda) § 102-75.1085 When using a delegation of real property disposal authority under this subpart, is USDA required to report excess...

  14. 7 CFR 3.45 - USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false USDA payment authorizing agency offset of pro rata... Secretary of Agriculture DEBT MANAGEMENT Administrative Offset § 3.45 USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor participates. (a) A USDA payment...

  15. 7 CFR 3.45 - USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false USDA payment authorizing agency offset of pro rata... Secretary of Agriculture DEBT MANAGEMENT Administrative Offset § 3.45 USDA payment authorizing agency offset of pro rata share of payments due entity in which debtor participates. (a) A USDA payment...

  16. High levels of exotic armored scales on imported avocados raise concerns regarding USDA-APHIS' phytosanitary risk assessment.

    PubMed

    Morse, J G; Rugman-Jones, P F; Watson, G W; Robinson, L J; Bi, J L; Stouthamer, R

    2009-06-01

    Between 1914 and 2007, a quarantine protected California avocado, Persea americana Mill., groves from pests that might be introduced into the state along with fresh, imported avocados. Soon after Mexican avocados were first allowed entry on 1 February 2007, live specimens of several species of armored scales (Hemiptera: Diaspididae) not believed to be present in California were detected on 'Hass' avocados entering the state from Mexico. Initially, the California Department of Food and Agriculture (CDFA) prevented avocados infested with these scales from entering the state or required that they be fumigated with an approved treatment such as methyl bromide. After a Science Advisory Panel meeting in May 2007, U.S. Department of Agriculture-Animal and Plant Health Inspection Service (USDA-APHIS) reaffirmed its position that armored scales on shipments of fruit for consumption (including avocados) pose a "low risk" for pest establishment. In compliance with APHIS protocols, as of 18 July 2007, CDFA altered its policy to allow shipments of scale-infested avocados into the state without treatment. Here, we report on sampling Mexican avocados over an 8-mo period, September 2007-April 2008. An estimated 67 million Mexican Hass avocados entered California over this period. Based on samples from 140 trucks containing approximately 15.6% of this volume of fruit, we estimate that approximately 47.6 million live, sessile armored scales and an additional 20.1 million live eggs and crawlers were imported. We found eight probable species of armored scales in the samples, seven of these are not believed to occur in California; 89.3% of the live scales were Abgrallaspis aguacatae Evans, Watson and Miller, a recently described species. In contrast to the USDA-APHIS opinion, we believe the volume of shipments and levels of live scales they contain present a significant risk to California's US$300 million avocado industry and to other crops that might become infested by one or more of

  17. Final work plan : investigation of potential contamination at the former CCC/USDA grain storage facility in Hanover, Kansas.

    SciTech Connect

    LaFreniere, L. M.; Environmental Science Division

    2008-11-19

    The Commodity Credit Corporation (CCC), an agency of the U.S. Department of Agriculture (USDA), operated a grain storage facility at the northeastern edge of the city of Hanover, Kansas, from 1950 until the early 1970s. During this time, commercial grain fumigants containing carbon tetrachloride were in common use by the grain storage industry to preserve grain in their facilities. In February 1998, trace to low levels of carbon tetrachloride (below the maximum contaminant level [MCL] of 5.0 {micro}g/L) were detected in two private wells near the former grain storage facility at Hanover, as part of a statewide USDA private well sampling program that was implemented by the Kansas Department of Health and Environment (KDHE) near former CCC/USDA facilities. In April 2007, the CCC/USDA collected near-surface soil samples at 1.8-2 ft BGL (below ground level) at 61 locations across the former CCC/USDA facility. All soil samples were analyzed by the rigorous gas chromatograph-mass spectrometer analytical method (purge-and-trap method). No contamination was found in soil samples above the reporting limit of 10 {micro}g/kg. In July 2007, the CCC/USDA sampled indoor air at nine residences on or adjacent to its former facility to address the residents concerns regarding vapor intrusion. Low levels of carbon tetrachloride were detected at four of the nine homes. Because carbon tetrachloride found in private wells and indoor air at the site might be linked to historical use of fumigants containing carbon tetrachloride at its former grain storage facility, the CCC/USDA is proposing to conduct an investigation to determine the source and extent of the carbon tetrachloride contamination associated with the former facility. This investigation will be conducted in accordance with the intergovernmental agreement between the KDHE and the Farm Service Agency (FSA) of the USDA. The investigation at Hanover will be performed, on behalf of the CCC/USDA, by the Environmental Science

  18. User services available from USDA'S aerial photography field office

    NASA Technical Reports Server (NTRS)

    Dickson, R. A.

    1975-01-01

    APFO furnishes LANDSAT imagery and supporting NASA aircraft imagery to NASA-funded principal investigators who are working within the agriculture discipline. The office holds and reproduces Skylab imagery and a variety of aircraft photography (including infrared) from various government agencies. Available products are listed. Other topics discussed include quality control of photographic materials, analytical aerotriangulation, and photographic processes.

  19. Mycotoxin Management Studies by USDA-ARS, NCAUR in 2009

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Studies again included several popcorn fields in 2009 in order to continue gathering data for modification of the previously developed management strategies for mycotoxins in field corn (including the mycotoxin predictive computer program). Without an attempt for optimization, the field corn model ...

  20. USDA-ARS National Sedimentation Laboratory: A historic perspective

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sediment from soil erosion is conceded by most authorities to be the largest single stream pollutant. Physical damage from sediment include reservoir storage loss, navigation channel filling, stream channel morphology alterations, ecological impacts, and clogging of drainage pathways. Ultimately, so...