Science.gov

Sample records for acquisition image analysis

  1. Rock fracture image acquisition and analysis

    NASA Astrophysics Data System (ADS)

    Wang, W.; Zongpu, Jia; Chen, Liwan

    2007-12-01

    As a cooperation project between Sweden and China, this paper presents: rock fracture image acquisition and analysis. Rock fracture images are acquired by using UV light illumination and visible optical illumination. To present fracture network reasonable, we set up some models to characterize the network, based on the models, we used Best fit Ferret method to auto-determine fracture zone, then, through skeleton fractures to obtain endpoints, junctions, holes, particles, and branches. Based on the new parameters and a part of common parameters, the fracture network density, porosity, connectivity and complexities can be obtained, and the fracture network is characterized. In the following, we first present a basic consideration and basic parameters for fractures (Primary study of characteristics of rock fractures), then, set up a model for fracture network analysis (Fracture network analysis), consequently to use the model to analyze fracture network with different images (Two dimensional fracture network analysis based on slices), and finally give conclusions and suggestions.

  2. Infrared image acquisition system for vein pattern analysis

    NASA Astrophysics Data System (ADS)

    Castro-Ortega, R.; Toxqui-Quitl, C.; Padilla-Vivanco, A.; Solís-Villarreal, J.

    2016-09-01

    The physical shape of the hand vascular distribution contains useful information that can be used for identifying and authenticating purposes; which provide a high level of security as a biometric. Furthermore, this pattern can be used widely in health field such as venography and venipuncture. In this paper, we analyze different IR imaging systems in order to obtain high visibility images of the hand vein pattern. The images are acquired in the range of 400 nm to 1300 nm, using infrared and thermal cameras. For the first image acquisition system, we use a CCD camera and a light source with peak emission in the 880 nm obtaining the images by reflection. A second system consists only of a ThermaCAM P65 camera acquiring the naturally emanating infrared light from the hand. A method of digital image analysis is implemented using Contrast Limited Adaptive Histogram Equalization (CLAHE) to remove noise. Subsequently, adaptive thresholding and mathematical morphology operations are implemented to get the vein pattern distribution.

  3. Troubleshooting digital macro photography for image acquisition and the analysis of biological samples.

    PubMed

    Liepinsh, Edgars; Kuka, Janis; Dambrova, Maija

    2013-01-01

    For years, image acquisition and analysis have been an important part of life science experiments to ensure the adequate and reliable presentation of research results. Since the development of digital photography and digital planimetric methods for image analysis approximately 20 years ago, new equipment and technologies have emerged, which have increased the quality of image acquisition and analysis. Different techniques are available to measure the size of stained tissue samples in experimental animal models of disease; however, the most accurate method is digital macro photography with software that is based on planimetric analysis. In this study, we described the methodology for the preparation of infarcted rat heart and brain tissue samples before image acquisition, digital macro photography techniques and planimetric image analysis. These methods are useful in the macro photography of biological samples and subsequent image analysis. In addition, the techniques that are described in this study include the automated analysis of digital photographs to minimize user input and exclude the risk of researcher-generated errors or bias during image analysis.

  4. Image Acquisition Context

    PubMed Central

    Bidgood, W. Dean; Bray, Bruce; Brown, Nicolas; Mori, Angelo Rossi; Spackman, Kent A.; Golichowski, Alan; Jones, Robert H.; Korman, Louis; Dove, Brent; Hildebrand, Lloyd; Berg, Michael

    1999-01-01

    Objective: To support clinically relevant indexing of biomedical images and image-related information based on the attributes of image acquisition procedures and the judgments (observations) expressed by observers in the process of image interpretation. Design: The authors introduce the notion of “image acquisition context,” the set of attributes that describe image acquisition procedures, and present a standards-based strategy for utilizing the attributes of image acquisition context as indexing and retrieval keys for digital image libraries. Methods: The authors' indexing strategy is based on an interdependent message/terminology architecture that combines the Digital Imaging and Communication in Medicine (DICOM) standard, the SNOMED (Systematized Nomenclature of Human and Veterinary Medicine) vocabulary, and the SNOMED DICOM microglossary. The SNOMED DICOM microglossary provides context-dependent mapping of terminology to DICOM data elements. Results: The capability of embedding standard coded descriptors in DICOM image headers and image-interpretation reports improves the potential for selective retrieval of image-related information. This favorably affects information management in digital libraries. PMID:9925229

  5. Quantitative assessment of the impact of biomedical image acquisition on the results obtained from image analysis and processing

    PubMed Central

    2014-01-01

    Introduction Dedicated, automatic algorithms for image analysis and processing are becoming more and more common in medical diagnosis. When creating dedicated algorithms, many factors must be taken into consideration. They are associated with selecting the appropriate algorithm parameters and taking into account the impact of data acquisition on the results obtained. An important feature of algorithms is the possibility of their use in other medical units by other operators. This problem, namely operator’s (acquisition) impact on the results obtained from image analysis and processing, has been shown on a few examples. Material and method The analysed images were obtained from a variety of medical devices such as thermal imaging, tomography devices and those working in visible light. The objects of imaging were cellular elements, the anterior segment and fundus of the eye, postural defects and others. In total, almost 200'000 images coming from 8 different medical units were analysed. All image analysis algorithms were implemented in C and Matlab. Results For various algorithms and methods of medical imaging, the impact of image acquisition on the results obtained is different. There are different levels of algorithm sensitivity to changes in the parameters, for example: (1) for microscope settings and the brightness assessment of cellular elements there is a difference of 8%; (2) for the thyroid ultrasound images there is a difference in marking the thyroid lobe area which results in a brightness assessment difference of 2%. The method of image acquisition in image analysis and processing also affects: (3) the accuracy of determining the temperature in the characteristic areas on the patient’s back for the thermal method - error of 31%; (4) the accuracy of finding characteristic points in photogrammetric images when evaluating postural defects – error of 11%; (5) the accuracy of performing ablative and non-ablative treatments in cosmetology - error of 18

  6. Asynchronous data acquisition and on-the-fly analysis of dose fractionated cryoEM images by UCSFImage.

    PubMed

    Li, Xueming; Zheng, Shawn; Agard, David A; Cheng, Yifan

    2015-11-01

    Newly developed direct electron detection cameras have a high image output frame rate that enables recording dose fractionated image stacks of frozen hydrated biological samples by electron cryomicroscopy (cryoEM). Such novel image acquisition schemes provide opportunities to analyze cryoEM data in ways that were previously impossible. The file size of a dose fractionated image stack is 20-60 times larger than that of a single image. Thus, efficient data acquisition and on-the-fly analysis of a large number of dose-fractionated image stacks become a serious challenge to any cryoEM data acquisition system. We have developed a computer-assisted system, named UCSFImage4, for semi-automated cryo-EM image acquisition that implements an asynchronous data acquisition scheme. This facilitates efficient acquisition, on-the-fly motion correction, and CTF analysis of dose fractionated image stacks with a total time of ∼60s/exposure. Here we report the technical details and configuration of this system.

  7. Automated ship image acquisition

    NASA Astrophysics Data System (ADS)

    Hammond, T. R.

    2008-04-01

    The experimental Automated Ship Image Acquisition System (ASIA) collects high-resolution ship photographs at a shore-based laboratory, with minimal human intervention. The system uses Automatic Identification System (AIS) data to direct a high-resolution SLR digital camera to ship targets and to identify the ships in the resulting photographs. The photo database is then searchable using the rich data fields from AIS, which include the name, type, call sign and various vessel identification numbers. The high-resolution images from ASIA are intended to provide information that can corroborate AIS reports (e.g., extract identification from the name on the hull) or provide information that has been omitted from the AIS reports (e.g., missing or incorrect hull dimensions, cargo, etc). Once assembled into a searchable image database, the images can be used for a wide variety of marine safety and security applications. This paper documents the author's experience with the practicality of composing photographs based on AIS reports alone, describing a number of ways in which this can go wrong, from errors in the AIS reports, to fixed and mobile obstructions and multiple ships in the shot. The frequency with which various errors occurred in automatically-composed photographs collected in Halifax harbour in winter time were determined by manual examination of the images. 45% of the images examined were considered of a quality sufficient to read identification markings, numbers and text off the entire ship. One of the main technical challenges for ASIA lies in automatically differentiating good and bad photographs, so that few bad ones would be shown to human users. Initial attempts at automatic photo rating showed 75% agreement with manual assessments.

  8. Colony image acquisition and segmentation

    NASA Astrophysics Data System (ADS)

    Wang, W. X.

    2007-12-01

    For counting of both colonies and plaques, there is a large number of applications including food, dairy, beverages, hygiene, environmental monitoring, water, toxicology, sterility testing, AMES testing, pharmaceuticals, paints, sterile fluids and fungal contamination. Recently, many researchers and developers have made efforts for this kind of systems. By investigation, some existing systems have some problems. The main problems are image acquisition and image segmentation. In order to acquire colony images with good quality, an illumination box was constructed as: the box includes front lightning and back lightning, which can be selected by users based on properties of colony dishes. With the illumination box, lightning can be uniform; colony dish can be put in the same place every time, which make image processing easy. The developed colony image segmentation algorithm consists of the sub-algorithms: (1) image classification; (2) image processing; and (3) colony delineation. The colony delineation algorithm main contain: the procedures based on grey level similarity, on boundary tracing, on shape information and colony excluding. In addition, a number of algorithms are developed for colony analysis. The system has been tested and satisfactory.

  9. Data acquisition and analysis for the energy-subtraction Compton scatter camera for medical imaging

    NASA Astrophysics Data System (ADS)

    Khamzin, Murat Kamilevich

    In response to the shortcomings of the Anger camera currently being used in conventional SPECT, particularly the trade-off between sensitivity and spatial resolution, a novel energy-subtraction Compton scatter camera, or the ESCSC, has been proposed. A successful clinical implementation of the ESCSC could revolutionize the field of SPECT. Features of this camera include utilization of silicon and CdZnTe detectors in primary and secondary detector systems, list-mode time stamping data acquisition, modular architecture, and post-acquisition data analysis. Previous ESCSC studies were based on Monte Carlo modeling. The objective of this work is to test the theoretical framework developed in previous studies by developing the data acquisition and analysis techniques necessary to implement the ESCSC. The camera model working in list-mode with time stamping was successfully built and tested thus confirming potential of the ESCSC that was predicted in previous simulation studies. The obtained data were processed during the post-acquisition data analysis based on preferred event selection criteria. Along with the construction of a camera model and proving the approach, the post-acquisition data analysis was further extended to include preferred event weighting based on the likelihood of a preferred event to be a true preferred event. While formulated to show ESCSC capabilities, the results of this study are important for any Compton scatter camera implementation as well as for coincidence data acquisition systems in general.

  10. Image acquisitions, processing and analysis in the process of obtaining characteristics of horse navicular bone

    NASA Astrophysics Data System (ADS)

    Zaborowicz, M.; Włodarek, J.; Przybylak, A.; Przybył, K.; Wojcieszak, D.; Czekała, W.; Ludwiczak, A.; Boniecki, P.; Koszela, K.; Przybył, J.; Skwarcz, J.

    2015-07-01

    The aim of this study was investigate the possibility of using methods of computer image analysis for the assessment and classification of morphological variability and the state of health of horse navicular bone. Assumption was that the classification based on information contained in the graphical form two-dimensional digital images of navicular bone and information of horse health. The first step in the research was define the classes of analyzed bones, and then using methods of computer image analysis for obtaining characteristics from these images. This characteristics were correlated with data concerning the animal, such as: side of hooves, number of navicular syndrome (scale 0-3), type, sex, age, weight, information about lace, information about heel. This paper shows the introduction to the study of use the neural image analysis in the diagnosis of navicular bone syndrome. Prepared method can provide an introduction to the study of non-invasive way to assess the condition of the horse navicular bone.

  11. Uav Photogrammetry with Oblique Images: First Analysis on Data Acquisition and Processing

    NASA Astrophysics Data System (ADS)

    Aicardi, I.; Chiabrando, F.; Grasso, N.; Lingua, A. M.; Noardo, F.; Spanò, A.

    2016-06-01

    In recent years, many studies revealed the advantages of using airborne oblique images for obtaining improved 3D city models (e.g. including façades and building footprints). Expensive airborne cameras, installed on traditional aerial platforms, usually acquired the data. The purpose of this paper is to evaluate the possibility of acquire and use oblique images for the 3D reconstruction of a historical building, obtained by UAV (Unmanned Aerial Vehicle) and traditional COTS (Commercial Off-the-Shelf) digital cameras (more compact and lighter than generally used devices), for the realization of high-level-of-detail architectural survey. The critical issues of the acquisitions from a common UAV (flight planning strategies, ground control points, check points distribution and measurement, etc.) are described. Another important considered aspect was the evaluation of the possibility to use such systems as low cost methods for obtaining complete information from an aerial point of view in case of emergency problems or, as in the present paper, in the cultural heritage application field. The data processing was realized using SfM-based approach for point cloud generation: different dense image-matching algorithms implemented in some commercial and open source software were tested. The achieved results are analysed and the discrepancies from some reference LiDAR data are computed for a final evaluation. The system was tested on the S. Maria Chapel, a part of the Novalesa Abbey (Italy).

  12. Acquisition and Analysis of Dynamic Responses of a Historic Pedestrian Bridge using Video Image Processing

    NASA Astrophysics Data System (ADS)

    O'Byrne, Michael; Ghosh, Bidisha; Schoefs, Franck; O'Donnell, Deirdre; Wright, Robert; Pakrashi, Vikram

    2015-07-01

    Video based tracking is capable of analysing bridge vibrations that are characterised by large amplitudes and low frequencies. This paper presents the use of video images and associated image processing techniques to obtain the dynamic response of a pedestrian suspension bridge in Cork, Ireland. This historic structure is one of the four suspension bridges in Ireland and is notable for its dynamic nature. A video camera is mounted on the river-bank and the dynamic responses of the bridge have been measured from the video images. The dynamic response is assessed without the need of a reflector on the bridge and in the presence of various forms of luminous complexities in the video image scenes. Vertical deformations of the bridge were measured in this regard. The video image tracking for the measurement of dynamic responses of the bridge were based on correlating patches in time-lagged scenes in video images and utilisinga zero mean normalised cross correlation (ZNCC) metric. The bridge was excited by designed pedestrian movement and by individual cyclists traversing the bridge. The time series data of dynamic displacement responses of the bridge were analysedto obtain the frequency domain response. Frequencies obtained from video analysis were checked against accelerometer data from the bridge obtained while carrying out the same set of experiments used for video image based recognition.

  13. Acquisition and Analysis of Dynamic Responses of a Historic Pedestrian Bridge using Video Image Processing

    NASA Astrophysics Data System (ADS)

    O'Byrne, Michael; Ghosh, Bidisha; Schoefs, Franck; O'Donnell, Deirdre; Wright, Robert; Pakrashi, Vikram

    2015-07-01

    Video based tracking is capable of analysing bridge vibrations that are characterised by large amplitudes and low frequencies. This paper presents the use of video images and associated image processing techniques to obtain the dynamic response of a pedestrian suspension bridge in Cork, Ireland. This historic structure is one of the four suspension bridges in Ireland and is notable for its dynamic nature. A video camera is mounted on the river-bank and the dynamic responses of the bridge have been measured from the video images. The dynamic response is assessed without the need of a reflector on the bridge and in the presence of various forms of luminous complexities in the video image scenes. Vertical deformations of the bridge were measured in this regard. The video image tracking for the measurement of dynamic responses of the bridge were based on correlating patches in time-lagged scenes in video images and utilisinga zero mean normalisedcross correlation (ZNCC) metric. The bridge was excited by designed pedestrian movement and by individual cyclists traversing the bridge. The time series data of dynamic displacement responses of the bridge were analysedto obtain the frequency domain response. Frequencies obtained from video analysis were checked against accelerometer data from the bridge obtained while carrying out the same set of experiments used for video image based recognition.

  14. Recovering the dynamics of root growth and development using novel image acquisition and analysis methods

    PubMed Central

    Wells, Darren M.; French, Andrew P.; Naeem, Asad; Ishaq, Omer; Traini, Richard; Hijazi, Hussein; Bennett, Malcolm J.; Pridmore, Tony P.

    2012-01-01

    Roots are highly responsive to environmental signals encountered in the rhizosphere, such as nutrients, mechanical resistance and gravity. As a result, root growth and development is very plastic. If this complex and vital process is to be understood, methods and tools are required to capture the dynamics of root responses. Tools are needed which are high-throughput, supporting large-scale experimental work, and provide accurate, high-resolution, quantitative data. We describe and demonstrate the efficacy of the high-throughput and high-resolution root imaging systems recently developed within the Centre for Plant Integrative Biology (CPIB). This toolset includes (i) robotic imaging hardware to generate time-lapse datasets from standard cameras under infrared illumination and (ii) automated image analysis methods and software to extract quantitative information about root growth and development both from these images and via high-resolution light microscopy. These methods are demonstrated using data gathered during an experimental study of the gravitropic response of Arabidopsis thaliana. PMID:22527394

  15. Recovering the dynamics of root growth and development using novel image acquisition and analysis methods.

    PubMed

    Wells, Darren M; French, Andrew P; Naeem, Asad; Ishaq, Omer; Traini, Richard; Hijazi, Hussein I; Hijazi, Hussein; Bennett, Malcolm J; Pridmore, Tony P

    2012-06-05

    Roots are highly responsive to environmental signals encountered in the rhizosphere, such as nutrients, mechanical resistance and gravity. As a result, root growth and development is very plastic. If this complex and vital process is to be understood, methods and tools are required to capture the dynamics of root responses. Tools are needed which are high-throughput, supporting large-scale experimental work, and provide accurate, high-resolution, quantitative data. We describe and demonstrate the efficacy of the high-throughput and high-resolution root imaging systems recently developed within the Centre for Plant Integrative Biology (CPIB). This toolset includes (i) robotic imaging hardware to generate time-lapse datasets from standard cameras under infrared illumination and (ii) automated image analysis methods and software to extract quantitative information about root growth and development both from these images and via high-resolution light microscopy. These methods are demonstrated using data gathered during an experimental study of the gravitropic response of Arabidopsis thaliana.

  16. Super-resolved image acquisition with full-field localization-based microscopy: theoretical analysis and evaluation

    NASA Astrophysics Data System (ADS)

    Son, Taehwang; Lee, Wonju; Kim, Donghyun

    2016-02-01

    We analyze and evaluate super-resolved image acquisition with full-field localization microscopy in which an individual signal sampled by localization may or may not be switched. For the analysis, Nyquist-Shannon sampling theorem based on ideal delta function was extended to sampling with unit pulse comb and surface-enhanced localized near-field that was numerically calculated with finite difference time domain. Sampling with unit pulse was investigated in Fourier domain where magnitude of baseband becomes larger than that of adjacent subband, i.e. aliasing effect is reduced owing to pulse width. Standard Lena image was employed as imaging target and a diffraction-limited optical system is assumed. A peak signal-to-noise ratio (PSNR) was introduced to evaluate the efficiency of image reconstruction quantitatively. When the target was sampled without switching by unit pulse as the sampling width and period are varied, PSNR increased eventually to 18.1 dB, which is the PSNR of a conventional diffraction-limited image. PSNR was found to increase with a longer pulse width due to reduced aliasing effect. When switching of individual sampling pulses was applied, blurry artifact outside the excited field is removed for each pulse and PSNR soars to 25.6 dB with a shortened pulse period, i.e. effective resolution of 72 nm is obtained, which can further be decreased.

  17. Analysis of Crack Image Recognition Characteristics in Concrete Structures Depending on the Illumination and Image Acquisition Distance through Outdoor Experiments

    PubMed Central

    Cho, Hyun-Woo; Yoon, Hyuk-Jin; Yoon, Jae-Chan

    2016-01-01

    The effects of illumination and shooting distance on crack image recognition were investigated by examining cracks in images taken with a camera. In order to examine the effects, images of cracks in a concrete structure taken while varying the illumination and shooting distance in an outdoor environment were analyzed. The images were acquired at a daytime illumination of 52,000 lx and a night illumination of 13 lx. The crack specimen images produced for the experiment were taken by increasing the shooting distance from 5 m to 100 m in each illumination. On the basis of the analysis on the modulation transfer function (MTF) and contrast sensitivity of the crack images, the effects of illumination and shooting distance on the sharpness of the crack images were investigated. The minimum crack widths that can be identified under each illumination were analyzed using MTF10 and Weber contrast 0.1, respectively. It was found that as the shooting distance increases, the effects of illumination on crack recognition become greater. PMID:27782056

  18. Graphical user interface for image acquisition and processing

    DOEpatents

    Goldberg, Kenneth A.

    2002-01-01

    An event-driven GUI-based image acquisition interface for the IDL programming environment designed for CCD camera control and image acquisition directly into the IDL environment where image manipulation and data analysis can be performed, and a toolbox of real-time analysis applications. Running the image acquisition hardware directly from IDL removes the necessity of first saving images in one program and then importing the data into IDL for analysis in a second step. Bringing the data directly into IDL creates an opportunity for the implementation of IDL image processing and display functions in real-time. program allows control over the available charge coupled device (CCD) detector parameters, data acquisition, file saving and loading, and image manipulation and processing, all from within IDL. The program is built using IDL's widget libraries to control the on-screen display and user interface.

  19. Three-dimensional model acquisition using rotational stereo and image focus analysis

    NASA Astrophysics Data System (ADS)

    Lin, Huei-Yung; Subbarao, Murali

    2001-02-01

    We present a digital vision system for acquiring the complete 3D model of an object from multiple views. The system uses image focus analysis to obtain a rough 3D shape of each view of an object and also the corresponding focused image or texture map. The rough 3D shape is used in a rotational stereo algorithm to obtain a more accurate measurement of 3D shape. The rotational stereo involves rotating the object by a small angle to obtain stereo images. It offers some important advantages compared to conventional stereo. A single camera is used instead of two, the stereo matching is easier as the field of view remains the same for the camera (but the object is rotated), and camera calibration is easier since a single stationary camera is used. The 3D shape and the corresponding texture map are measured for 4 views of the object at 90 degree angular intervals. These partial shapes and texture maps are integrated to obtain a complete 360 degree model of the object. The theory and algorithms underlying rotational-stereo and integration of partial 3D models are presented. The system can acquire the 3D model (which includes the 3D shape and the corresponding image texture) of a simple object within a 300mm x 300mm x 300mm volume placed about 600 mm from the camera. The complete model is displayed using a 3D graphics rendering software (Apple's QuickDraw 3D Viewer). Both computational algorithms and experimental results on several objects are presented.

  20. Acquisition and applications of 3D images

    NASA Astrophysics Data System (ADS)

    Sterian, Paul; Mocanu, Elena

    2007-08-01

    The moiré fringes method and their analysis up to medical and entertainment applications are discussed in this paper. We describe the procedure of capturing 3D images with an Inspeck Camera that is a real-time 3D shape acquisition system based on structured light techniques. The method is a high-resolution one. After processing the images, using computer, we can use the data for creating laser fashionable objects by engraving them with a Q-switched Nd:YAG. In medical field we mention the plastic surgery and the replacement of X-Ray especially in pediatric use.

  1. Acquisition hardware for digital imaging.

    PubMed

    Widmer, William R

    2008-01-01

    Use of digital radiography is growing rapidly in veterinary medicine. Two basic digital imaging systems are available, computed radiography (CR) and direct digital radiography (DDR). Computed radiographic detectors use a two-step process for image capture and processing. Image capture is by X-ray sensitive phosphors in the image plate. The image plate reader transforms the latent phosphor image to light photons that are converted to an analog electrical signal. An analog to digital converter is used to digitize the electrical signal before computer analysis. Direct digital detectors provide digital data by direct readout after image capture--a reader unnecessary. Types of DDR detectors are flat panel detectors and charge coupled device (CCD) detectors. Flat panel detectors are composed of layers of semiconductors for image capture with transistor and microscopic circuitry embedded in a pixel array. Direct converting flat panel detectors convert incident X-rays directly into electrical charges. Indirect detectors convert X-rays to visible light, then to electrical charges. All flat panel detectors send a digitized electrical signal to a computer using a direct link. Charge coupled device detectors have a small chip similar to those used in digital cameras. A scintillator first converts X-rays to a light signal that is minified by an optical system before reaching the chip. The chip sends a digital signal directly to a computer. Both CR and DDR provide quality diagnostic images. CR is a mature technology while DDR is an emerging technology.

  2. EMOTIONS AND IMAGES IN LANGUAGE--A LEARNING ANALYSIS OF THEIR ACQUISITION AND FUNCTION.

    ERIC Educational Resources Information Center

    STAATS, ARTHUR W.

    THIS ARTICLE PRESENTED THEORETICAL AND EXPERIMENTAL ANALYSES CONCERNING IMPORTANT ASPECTS OF LANGUAGE. IT WAS SUGGESTED THAT A LEARNING THEORY WHICH INEGRATES INSTRUMENTAL AND CLASSICAL CONDITIONING, CUTTING ACROSS THEORETICAL LINES, COULD SERVE AS THE BASIS FOR A COMPREHENSIVE THEORY OF LANGUAGE ACQUISITION AND FUNCTION. THE PAPER ILLUSTRATED THE…

  3. Functional MRI using regularized parallel imaging acquisition.

    PubMed

    Lin, Fa-Hsuan; Huang, Teng-Yi; Chen, Nan-Kuei; Wang, Fu-Nien; Stufflebeam, Steven M; Belliveau, John W; Wald, Lawrence L; Kwong, Kenneth K

    2005-08-01

    Parallel MRI techniques reconstruct full-FOV images from undersampled k-space data by using the uncorrelated information from RF array coil elements. One disadvantage of parallel MRI is that the image signal-to-noise ratio (SNR) is degraded because of the reduced data samples and the spatially correlated nature of multiple RF receivers. Regularization has been proposed to mitigate the SNR loss originating due to the latter reason. Since it is necessary to utilize static prior to regularization, the dynamic contrast-to-noise ratio (CNR) in parallel MRI will be affected. In this paper we investigate the CNR of regularized sensitivity encoding (SENSE) acquisitions. We propose to implement regularized parallel MRI acquisitions in functional MRI (fMRI) experiments by incorporating the prior from combined segmented echo-planar imaging (EPI) acquisition into SENSE reconstructions. We investigated the impact of regularization on the CNR by performing parametric simulations at various BOLD contrasts, acceleration rates, and sizes of the active brain areas. As quantified by receiver operating characteristic (ROC) analysis, the simulations suggest that the detection power of SENSE fMRI can be improved by regularized reconstructions, compared to unregularized reconstructions. Human motor and visual fMRI data acquired at different field strengths and array coils also demonstrate that regularized SENSE improves the detection of functionally active brain regions.

  4. Functional MRI Using Regularized Parallel Imaging Acquisition

    PubMed Central

    Lin, Fa-Hsuan; Huang, Teng-Yi; Chen, Nan-Kuei; Wang, Fu-Nien; Stufflebeam, Steven M.; Belliveau, John W.; Wald, Lawrence L.; Kwong, Kenneth K.

    2013-01-01

    Parallel MRI techniques reconstruct full-FOV images from undersampled k-space data by using the uncorrelated information from RF array coil elements. One disadvantage of parallel MRI is that the image signal-to-noise ratio (SNR) is degraded because of the reduced data samples and the spatially correlated nature of multiple RF receivers. Regularization has been proposed to mitigate the SNR loss originating due to the latter reason. Since it is necessary to utilize static prior to regularization, the dynamic contrast-to-noise ratio (CNR) in parallel MRI will be affected. In this paper we investigate the CNR of regularized sensitivity encoding (SENSE) acquisitions. We propose to implement regularized parallel MRI acquisitions in functional MRI (fMRI) experiments by incorporating the prior from combined segmented echo-planar imaging (EPI) acquisition into SENSE reconstructions. We investigated the impact of regularization on the CNR by performing parametric simulations at various BOLD contrasts, acceleration rates, and sizes of the active brain areas. As quantified by receiver operating characteristic (ROC) analysis, the simulations suggest that the detection power of SENSE fMRI can be improved by regularized reconstructions, compared to unregularized reconstructions. Human motor and visual fMRI data acquired at different field strengths and array coils also demonstrate that regularized SENSE improves the detection of functionally active brain regions. PMID:16032694

  5. Acquisition and analysis of spectral image data by linear un-mixing, cluster computing and a novel spectral imager

    NASA Astrophysics Data System (ADS)

    Barber, Paul R.; Edens, Richard J.; Vojnovic, B.

    2005-03-01

    We describe how spectral imaging, linear un-mixing and cluster computing have been combined to aid biomedical researchers and allow the spatial segmentation and quantitative analysis of immunohistochemically stained tissue section images. A novel cost-effective spectral imager, with a bandwidth of 15 nm between 400 and 700 nm, allows us to record both spatial and spectral data from absorptive and fluorescent chemical probes. The linear un-mixing of this data separates the stain distributions revealing areas of co-localisation and extracts quantitative values of optical density. This has been achieved at the single-pixel level of an image by non-negative least squares fitting. This process can be computationally expensive but great processing speed increases have been achieved through the use of cluster computing. We describe how several personal computers, running Microsoft WindowsXP, can be used in parallel, linked by the MPI (Message Passing Interface) standard. We describe how the free MPICH libraries have been incorporated into our spectral imaging application under the C language and how this has been extended to support features of MPI2 via the commercial WMPI II libraries. A cluster of 8 processors, in 4 dual-Athlon-2600+ computers, offered a speed up of a factor of 5 compared to a singleton. This includes the time required to transfer the data throughout the cluster and reflects a processing efficiency of 0.62 (a Cluster Efficacy of 3.0). The cluster was based on a 1000Base-T Ethernet network and appears to be scalable efficiently beyond 8 processors.

  6. A Quantitative Three-Dimensional Image Analysis Tool for Maximal Acquisition of Spatial Heterogeneity Data.

    PubMed

    Allenby, Mark C; Misener, Ruth; Panoskaltsis, Nicki; Mantalaris, Athanasios

    2017-02-01

    Three-dimensional (3D) imaging techniques provide spatial insight into environmental and cellular interactions and are implemented in various fields, including tissue engineering, but have been restricted by limited quantification tools that misrepresent or underutilize the cellular phenomena captured. This study develops image postprocessing algorithms pairing complex Euclidean metrics with Monte Carlo simulations to quantitatively assess cell and microenvironment spatial distributions while utilizing, for the first time, the entire 3D image captured. Although current methods only analyze a central fraction of presented confocal microscopy images, the proposed algorithms can utilize 210% more cells to calculate 3D spatial distributions that can span a 23-fold longer distance. These algorithms seek to leverage the high sample cost of 3D tissue imaging techniques by extracting maximal quantitative data throughout the captured image.

  7. Image analysis in modern ophthalmology: from acquisition to computer assisted diagnosis and telemedicine

    NASA Astrophysics Data System (ADS)

    Marrugo, Andrés G.; Millán, María S.; Cristóbal, Gabriel; Gabarda, Salvador; Sorel, Michal; Sroubek, Filip

    2012-06-01

    Medical digital imaging has become a key element of modern health care procedures. It provides visual documentation and a permanent record for the patients, and most important the ability to extract information about many diseases. Modern ophthalmology thrives and develops on the advances in digital imaging and computing power. In this work we present an overview of recent image processing techniques proposed by the authors in the area of digital eye fundus photography. Our applications range from retinal image quality assessment to image restoration via blind deconvolution and visualization of structural changes in time between patient visits. All proposed within a framework for improving and assisting the medical practice and the forthcoming scenario of the information chain in telemedicine.

  8. Data acquisition and analysis of mammography images at the NSLS June--August 1995

    SciTech Connect

    Arfelli, F.; Burns, C.; Chapman, D. |

    1995-12-31

    At Brookhaven National Laboratory mammography experiments are being carried out at the X27C R and D beamline of the National Synchrotron Light Source using a monochromatic x-ray beam in order to explore the potential of monoenergetic photons for mammographic imaging. In two different periods of beamtime the authors have performed preliminary studies of mammographic imaging using a monochromatic synchrotron radiation source. They used both phantom objects and real tissue samples. Qualitative studies with the contrast-detail phantom show good agreement when compared with the theoretical contrast. As expected, the contrast is higher if the energy is lower. The results show an improved contrast with energies 18 keV and lower compared to images obtained from conventional polyenergetic x-ray imaging systems. The results also show that for similar imaging conditions the monoenergetic mean glandular dose is less than that from polyenergetic sources. This is due both to the increased sensitivity of the image plate detectors and to actual reductions of dose for truly monochromatic beams.

  9. Applications Of Digital Image Acquisition In Anthropometry

    NASA Astrophysics Data System (ADS)

    Woolford, Barbara; Lewis, James L.

    1981-10-01

    Anthropometric data on reach and mobility have traditionally been collected by time consuming and relatively inaccurate manual methods. Three dimensional digital image acquisition promises to radically increase the speed and ease of data collection and analysis. A three-camera video anthropometric system for collecting position, velocity, and force data in real time is under development for the Anthropometric Measurement Laboratory at NASA's Johnson Space Center. The use of a prototype of this system for collecting data on reach capabilities and on lateral stability is described. Two extensions of this system are planned.

  10. Optimisation of acquisition time in bioluminescence imaging

    NASA Astrophysics Data System (ADS)

    Taylor, Shelley L.; Mason, Suzannah K. G.; Glinton, Sophie; Cobbold, Mark; Styles, Iain B.; Dehghani, Hamid

    2015-03-01

    Decreasing the acquisition time in bioluminescence imaging (BLI) and bioluminescence tomography (BLT) will enable animals to be imaged within the window of stable emission of the bioluminescent source, a higher imaging throughput and minimisation of the time which an animal is anaesthetised. This work investigates, through simulation using a heterogeneous mouse model, two methods of decreasing acquisition time: 1. Imaging at fewer wavelengths (a reduction from five to three); and 2. Increasing the bandwidth of filters used for imaging. The results indicate that both methods are viable ways of decreasing the acquisition time without a loss in quantitative accuracy. Importantly, when choosing imaging wavelengths, the spectral attenuation of tissue and emission spectrum of the source must be considered, in order to choose wavelengths at which a high signal can be achieved. Additionally, when increasing the bandwidth of the filters used for imaging, the bandwidth must be accounted for in the reconstruction algorithm.

  11. Coronary CTA: image acquisition and interpretation.

    PubMed

    Kerl, Josef Matthias; Hofmann, Lars K; Thilo, Christian; Vogl, Thomas J; Costello, Philip; Schoepf, U Joseph

    2007-02-01

    Computed tomography (CT) of the heart, because of ongoing technical refinement and intense scientific and clinical evaluation, has left the research realm and has matured into a clinical application that is about to fulfill its promise to replace invasive cardiac catheterization in some patient populations. By nature of its target, the continuously moving heart, CT coronary angiography is technically more challenging than other CT applications. Also, rapid technical development requires constant adaptation of acquisition protocols. Those challenges, however, are in no way insurmountable for users with knowledge of general CT technique. The intent of this communication is to provide for those interested in and involved with coronary CT angiography a step-by-step manual, introducing our approach to performing coronary CT angiography. Included are considerations regarding appropriate patient selection, patient medication, radiation protection, contrast enhancement, acquisition and reconstruction parameters, image display and analysis techniques and also the radiology report. Our recommendations are based on our experience which spans the evolution of multidetector-row CT for cardiac applications from its beginnings to the most current iterations of advanced acquisition modalities, which we believe herald the entrance of this test into routine clinical practice.

  12. Acquisition, Image and Data Compression.

    DTIC Science & Technology

    1983-04-30

    In Block 20, If different from Report) 1. SUPPLEMENTARY NOTES Presentations made at MILCOM󈨖 Optical Society 󈨗 SPIE󈨗 Optical Computing...Conference󈨗. 19. KEY WORDS (Continue on reverse aide It necessary end identify by block number) Spread Spectrum, Optical Transforms, Acquisition, Simulation...Tracking, PN Direct Sequence Frequency Hopping 20. ABSTRACT (Continue on reer. aide it nece-iary end Identify hy block nomber) This report discusses

  13. In Vivo Confocal Microscopy of the Cornea: New Developments in Image Acquisition, Reconstruction, and Analysis Using the HRT-Rostock Corneal Module.

    PubMed

    Petroll, W Matthew; Robertson, Danielle M

    2015-07-01

    The optical sectioning ability of confocal microscopy allows high magnification images to be obtained from different depths within a thick tissue specimen and is thus ideally suited to the study of intact tissue in living subjects. In vivo confocal microscopy has been used in a variety of corneal research and clinical applications since its development over 25 years ago. In this article we review the latest developments in quantitative corneal imaging with the Heidelberg Retinal Tomograph with Rostock Corneal Module (HRT-RCM). We provide an overview of the unique strengths and weaknesses of the HRT-RCM. We discuss techniques for performing 3-D imaging with the HRT-RCM, including hardware and software modifications that allow full-thickness confocal microscopy through-focusing (CMTF) of the cornea, which can provide quantitative measurements of corneal sublayer thicknesses, stromal cell and extracellular matrix backscatter, and depth-dependent changes in corneal keratocyte density. We also review current approaches for quantitative imaging of the subbasal nerve plexus, which require a combination of advanced image acquisition and analysis procedures, including wide-field mapping and 3-D reconstruction of nerve structures. The development of new hardware, software, and acquisition techniques continues to expand the number of applications of the HRT-RCM for quantitative in vivo corneal imaging at the cellular level. Knowledge of these rapidly evolving strategies should benefit corneal clinicians and basic scientists alike.

  14. In vivo confocal microscopy of the cornea: New developments in image acquisition, reconstruction and analysis using the HRT-Rostock Corneal Module

    PubMed Central

    Petroll, W. Matthew; Robertson, Danielle M.

    2015-01-01

    The optical sectioning ability of confocal microscopy allows high magnification images to be obtained from different depths within a thick tissue specimen, and is thus ideally suited to the study of intact tissue in living subjects. In vivo confocal microscopy has been used in a variety of corneal research and clinical applications since its development over 25 years ago. In this article we review the latest developments in quantitative corneal imaging with the Heidelberg Retinal Tomograph with Rostock Corneal Module (HRT-RCM). We provide an overview of the unique strengths and weaknesses of the HRT-RCM. We discuss techniques for performing 3-D imaging with the HRT-RCM, including hardware and software modifications that allow full thickness confocal microscopy through focusing (CMTF) of the cornea, which can provide quantitative measurements of corneal sublayer thicknesses, stromal cell and extracellular matrix backscatter, and depth dependent changes in corneal keratocyte density. We also review current approaches for quantitative imaging of the subbasal nerve plexus, which require a combination of advanced image acquisition and analysis procedures, including wide field mapping and 3-D reconstruction of nerve structures. The development of new hardware, software, and acquisition techniques continues to expand the number of applications of the HRT-RCM for quantitative in vivo corneal imaging at the cellular level. Knowledge of these rapidly evolving strategies should benefit corneal clinicians and basic scientists alike. PMID:25998608

  15. Image Acquisition in Real Time

    NASA Technical Reports Server (NTRS)

    2003-01-01

    In 1995, Carlos Jorquera left NASA s Jet Propulsion Laboratory (JPL) to focus on erasing the growing void between high-performance cameras and the requisite software to capture and process the resulting digital images. Since his departure from NASA, Jorquera s efforts have not only satisfied the private industry's cravings for faster, more flexible, and more favorable software applications, but have blossomed into a successful entrepreneurship that is making its mark with improvements in fields such as medicine, weather forecasting, and X-ray inspection. Formerly a JPL engineer who constructed imaging systems for spacecraft and ground-based astronomy projects, Jorquera is the founder and president of the three-person firm, Boulder Imaging Inc., based in Louisville, Colorado. Joining Jorquera to round out the Boulder Imaging staff are Chief Operations Engineer Susan Downey, who also gained experience at JPL working on space-bound projects including Galileo and the Hubble Space Telescope, and Vice President of Engineering and Machine Vision Specialist Jie Zhu Kulbida, who has extensive industrial and research and development experience within the private sector.

  16. Image acquisition system for a hospital enterprise

    NASA Astrophysics Data System (ADS)

    Moore, Stephen M.; Beecher, David E.

    1998-07-01

    Hospital enterprises are being created through mergers and acquisitions of existing hospitals. One area of interest in the PACS literature has been the integration of information systems and imaging systems. Hospital enterprises with multiple information and imaging systems provide new challenges to the integration task. This paper describes the requirements at the BJC Health System and a testbed system that is designed to acquire images from a number of different modalities and hospitals. This testbed system is integrated with Project Spectrum at BJC which is designed to provide a centralized clinical repository and a single desktop application for physician review of the patient chart (text, lab values, images).

  17. Effective GPR Data Acquisition and Imaging

    NASA Astrophysics Data System (ADS)

    Sato, M.

    2014-12-01

    We have demonstrated that dense GPR data acquisition typically antenna step increment less than 1/10 wave length can provide clear 3-dimeantiona subsurface images, and we created 3DGPR images. Now we are interested in developing GPR survey methodologies which required less data acquisition time. In order to speed up the data acquisition, we are studying efficient antenna positioning for GPR survey and 3-D imaging algorithm. For example, we have developed a dual sensor "ALIS", which combines GPR with metal detector (Electromagnetic Induction sensor) for humanitarian demining, which acquires GPR data by hand scanning. ALIS is a pulse radar system, which has a frequency range 0.5-3GHz.The sensor position tracking system has accuracy about a few cm, and the data spacing is typically more than a few cm, but it can visualize the mines, which has a diameter about 8cm. 2 systems of ALIS have been deployed by Cambodian Mine Action Center (CMAC) in mine fields in Cambodia since 2009 and have detected more than 80 buried land mines. We are now developing signal processing for an array type GPR "Yakumo". Yakumo is a SFCW radar system which is a multi-static radar, consisted of 8 transmitter antennas and 8 receiver antennas. We have demonstrated that the multi-static data acquisition is not only effective in data acquisition, but at the same time, it can increase the quality of GPR images. Archaeological survey by Yakumo in large areas, which are more than 100m by 100m have been conducted, for promoting recovery from Tsunami attacked East Japan in March 2011. With a conventional GPR system, we are developing an interpolation method of radar signals, and demonstrated that it can increase the quality of the radar images, without increasing the data acquisition points. When we acquire one dimensional GPR profile along a survey line, we can acquire relatively high density data sets. However, when we need to relocate the data sets along a "virtual" survey line, for example a

  18. Retinal Imaging and Image Analysis

    PubMed Central

    Abràmoff, Michael D.; Garvin, Mona K.; Sonka, Milan

    2011-01-01

    Many important eye diseases as well as systemic diseases manifest themselves in the retina. While a number of other anatomical structures contribute to the process of vision, this review focuses on retinal imaging and image analysis. Following a brief overview of the most prevalent causes of blindness in the industrialized world that includes age-related macular degeneration, diabetic retinopathy, and glaucoma, the review is devoted to retinal imaging and image analysis methods and their clinical implications. Methods for 2-D fundus imaging and techniques for 3-D optical coherence tomography (OCT) imaging are reviewed. Special attention is given to quantitative techniques for analysis of fundus photographs with a focus on clinically relevant assessment of retinal vasculature, identification of retinal lesions, assessment of optic nerve head (ONH) shape, building retinal atlases, and to automated methods for population screening for retinal diseases. A separate section is devoted to 3-D analysis of OCT images, describing methods for segmentation and analysis of retinal layers, retinal vasculature, and 2-D/3-D detection of symptomatic exudate-associated derangements, as well as to OCT-based analysis of ONH morphology and shape. Throughout the paper, aspects of image acquisition, image analysis, and clinical relevance are treated together considering their mutually interlinked relationships. PMID:21743764

  19. Detecting ordered small molecule drug aggregates in live macrophages: a multi-parameter microscope image data acquisition and analysis strategy

    PubMed Central

    Rzeczycki, Phillip; Yoon, Gi Sang; Keswani, Rahul K.; Sud, Sudha; Stringer, Kathleen A.; Rosania, Gus R.

    2017-01-01

    Following prolonged administration, certain orally bioavailable but poorly soluble small molecule drugs are prone to precipitate out and form crystal-like drug inclusions (CLDIs) within the cells of living organisms. In this research, we present a quantitative multi-parameter imaging platform for measuring the fluorescence and polarization diattenuation signals of cells harboring intracellular CLDIs. To validate the imaging system, the FDA-approved drug clofazimine (CFZ) was used as a model compound. Our results demonstrated that a quantitative multi-parameter microscopy image analysis platform can be used to study drug sequestering macrophages, and to detect the formation of ordered molecular aggregates formed by poorly soluble small molecule drugs in animals. PMID:28270989

  20. Material appearance acquisition from a single image

    NASA Astrophysics Data System (ADS)

    Zhang, Xu; Cui, Shulin; Cui, Hanwen; Yang, Lin; Wu, Tao

    2017-01-01

    The scope of this paper is to present a method of material appearance acquisition(MAA) from a single image. In this paper, material appearance is represented by spatially varying bidirectional reflectance distribution function(SVBRDF). Therefore, MAA can be reduced to the problem of recovery of each pixel's BRDF parameters from an original input image, which include diffuse coefficient, specular coefficient, normal and glossiness based on the Blinn-Phone model. In our method, the workflow of MAA includes five main phases: highlight removal, estimation of intrinsic images, shape from shading(SFS), initialization of glossiness and refining SVBRDF parameters based on IPOPT. The results indicate that the proposed technique can effectively extract the material appearance from a single image.

  1. An Analysis of Army Rapid Acquisition

    DTIC Science & Technology

    2015-09-01

    Strategy ........54 6. Value Center Six: Life Cycle Costs ............................................54 7. Value Center Seven: Lessons Learned...information assurance strategy ANPP Apple new product process AOA analysis of alternatives APB acquisition program baseline APC acquisition program...candidate APO Army Project Office APUC average procurement unit cost AS acquisition strategy ASOM acquisition system operating model BBP better

  2. Colony image acquisition and genetic segmentation algorithm and colony analyses

    NASA Astrophysics Data System (ADS)

    Wang, W. X.

    2012-01-01

    Colony anaysis is used in a large number of engineerings such as food, dairy, beverages, hygiene, environmental monitoring, water, toxicology, sterility testing. In order to reduce laboring and increase analysis acuracy, many researchers and developers have made efforts for image analysis systems. The main problems in the systems are image acquisition, image segmentation and image analysis. In this paper, to acquire colony images with good quality, an illumination box was constructed. In the box, the distances between lights and dishe, camra lens and lights, and camera lens and dishe are adjusted optimally. In image segmentation, It is based on a genetic approach that allow one to consider the segmentation problem as a global optimization,. After image pre-processing and image segmentation, the colony analyses are perfomed. The colony image analysis consists of (1) basic colony parameter measurements; (2) colony size analysis; (3) colony shape analysis; and (4) colony surface measurements. All the above visual colony parameters can be selected and combined together, used to make a new engineeing parameters. The colony analysis can be applied into different applications.

  3. Optimization of EFTEM image acquisition by using elastically filtered images for drift correction.

    PubMed

    Heil, Tobias; Kohl, Helmut

    2010-06-01

    Because of its high spatial resolution, energy-filtering transmission electron microscopy (EFTEM) has become widely used for the analysis of the chemical composition of nanostructures. To obtain the best spatial resolution, the precise correction of instrumental influences and the optimization of the data acquisition procedure are very important. In this publication, we discuss a modified image acquisition procedure that optimizes the acquisition process of the EFTEM images, especially for long exposure times and measurements that are affected by large spatial drift. To alleviate the blurring of the image caused by the spatial drift, we propose to take several EFTEM images with a shorter exposure time (sub-images) and merge these sub-images afterwards. To correct for the drift between these sub-images, elastically filtered images are acquired between two subsequent sub-images. These elastically filtered images are highly suitable for spatial drift correction based on the cross-correlation method. The use of the drift information between two elastically filtered images permits to merge the drift-corrected sub-images automatically and with high accuracy, resulting in sharper edges and an improved signal intensity in the final EFTEM image. Artefacts that are caused by prominent noise-peaks in the dark reference image have been suppressed by calculating the dark reference image from three images. Furthermore, using the information given by the elastically filtered images, it is possible to drift-correct a set of EFTEM images already during the acquisition. This simplifies the post-processing for elemental mapping and offers the possibility for active drift correction using the image shift function of the microscope, leading to an increased field of view.

  4. Phytoplankton Imaging and Analysis System: Instrumentation for Field and Laboratory Acquisition, Analysis and WWW/LAN-Based Sharing of Marine Phytoplankton Data (DURIP)

    DTIC Science & Technology

    2000-09-30

    networks (LAN), (3) quantifying size, shape, and other parameters of plankton cells and colonies via image analysis and image reconstruction, and (4) creating educational materials (e.g. lectures, videos etc.).

  5. New automated iris image acquisition method.

    PubMed

    Park, Kang Ryoung

    2005-02-10

    I propose a new iris image acquisition method based on wide- and narrow-view iris cameras. The narrow-view camera has the functionalities of automatic zooming, focusing, panning, and tilting based on the two-dimensional and three-dimensional eye positions detected from the wide- and narrow-view stereo cameras. By using the wide- and narrow-view iris cameras, I compute the user's gaze position, which is used for aligning the X-Y position of the user's eye, and I use the visible-light illuminator for fake-eye detection.

  6. Image acquisition system for traffic monitoring applications

    NASA Astrophysics Data System (ADS)

    Auty, Glen; Corke, Peter I.; Dunn, Paul; Jensen, Murray; Macintyre, Ian B.; Mills, Dennis C.; Nguyen, Hao; Simons, Ben

    1995-03-01

    An imaging system for monitoring traffic on multilane highways is discussed. The system, named Safe-T-Cam, is capable of operating 24 hours per day in all but extreme weather conditions and can capture still images of vehicles traveling up to 160 km/hr. Systems operating at different remote locations are networked to allow transmission of images and data to a control center. A remote site facility comprises a vehicle detection and classification module (VCDM), an image acquisition module (IAM) and a license plate recognition module (LPRM). The remote site is connected to the central site by an ISDN communications network. The remote site system is discussed in this paper. The VCDM consists of a video camera, a specialized exposure control unit to maintain consistent image characteristics, and a 'real-time' image processing system that processes 50 images per second. The VCDM can detect and classify vehicles (e.g. cars from trucks). The vehicle class is used to determine what data should be recorded. The VCDM uses a vehicle tracking technique to allow optimum triggering of the high resolution camera of the IAM. The IAM camera combines the features necessary to operate consistently in the harsh environment encountered when imaging a vehicle 'head-on' in both day and night conditions. The image clarity obtained is ideally suited for automatic location and recognition of the vehicle license plate. This paper discusses the camera geometry, sensor characteristics and the image processing methods which permit consistent vehicle segmentation from a cluttered background allowing object oriented pattern recognition to be used for vehicle classification. The image capture of high resolution images and the image characteristics required for the LPRMs automatic reading of vehicle license plates, is also discussed. The results of field tests presented demonstrate that the vision based Safe-T-Cam system, currently installed on open highways, is capable of producing automatic

  7. ISON Data Acquisition and Analysis Software

    NASA Astrophysics Data System (ADS)

    Kouprianov, Vladimir

    2013-08-01

    Since the first days of the ISON project, its success was strongly based on using advanced data analysis techniques and their implementation in software. Space debris studies and space surveillance in optical are very unique from the point of view of observation techniques and thus infer extremely specific requirements on sensor design and control and on initial data analysis, dictated mostly by fast apparent motion of space objects being studied. From the point of view of data acquisition and analysis software, this implies support for sophisticated scheduling, complex tracking, accurate timing, large fields of view, and undersampled CCD images with trailed sources. Here we present the historical outline, major goals and design concepts of the standard ISON data acquisition and analysis packages, and how they meet these requirements. Among these packages, the most important are: CHAOS telescope control system (TCS), its recent successor FORTE, and Apex II ‒ a platform for astronomical image analysis with focus on high-precision astrometry and photometry of fast-moving objects and transient phenomena. Development of these packages is supported by ISON, and they are now responsible for most of the raw data produced by the network. They are installed on nearly all sensors and are available to all participants of the ISON collaboration.

  8. Quantitative ADF STEM: acquisition, analysis and interpretation

    NASA Astrophysics Data System (ADS)

    Jones, L.

    2016-01-01

    Quantitative annular dark-field in the scanning transmission electron microscope (ADF STEM), where image intensities are used to provide composition and thickness measurements, has enjoyed a renaissance during the last decade. Now in a post aberration-correction era many aspects of the technique are being revisited. Here the recent progress and emerging best-practice for such aberration corrected quantitative ADF STEM is discussed including issues relating to proper acquisition of experimental data and its calibration, approaches for data analysis, the utility of such data, its interpretation and limitations.

  9. High-pitch dual-source CT coronary angiography: analysis of the impact on image quality of altered electrocardiography waves during data acquisition.

    PubMed

    Wang, Xiaoling; Fang, Jiliang; Tong, Haibin; Zhao, Qing; Song, Qingqiao; Luo, Ping; Xue, Chao; Zhang, Min; Yang, Shuhua; Wang, Qun; Wang, Ping; Shi, Fengxiang; Xu, Lei; Rong, Peijing

    2012-06-01

    Electrocardiography (ECG) "altered waves" sometimes occur during data acquisition when computed tomography coronary angiography (CTCA) is performed with the prospectively ECG-triggered high-pitch (Flash spiral) mode using a second-generation dual-source CT. The aim of this study was to assess the effect of the ECG altered waves on image quality. Seventy-three consecutive patients with stable sinus rhythm ≤ 65 beats per minute were retrospectively enrolled in this study. CTCA was performed using the Flash spiral mode in which the data acquisition was prospectively triggered at 60 % of the R-R interval and completed within one cardiac cycle. The ECG waves before and during data acquisition were analyzed for grouping purposes. Image quality was evaluated using a four-point scale (1 = best, 4 = unevaluatable). Thirty patients (group 1) were found to have ECG altered waves during data acquisition, while 43 patients (group 2) had ECG "stable waves." The altered waves were seen as the baseline drifting; the broad, erected, or inverted P wave or QRS complexes; and a new wave. However, the length of the R-R interval did not change during the data acquisition. There were no significant differences in image quality scores between the two groups on the per-patient (2 ± 0.87 vs. 2.2 ± 0.74, P = 0.273) or per-segment (1.27 ± 0.54 vs. 1.32 ± 0.55, P = 0.577) basis. There were no significant differences in coronary evaluatability as well (per-patient; 93.3 vs. 95.3 %, P = 0.352; per-segment; 99.4 vs. 99.6 %, P = 1.0). CTCA image quality is not affected by ECG altered waves during data acquisition using the Flash spiral mode in low and stable heart rate patients. Thus, the ECG altered waves are considered artifacts.

  10. Reproducible high-resolution multispectral image acquisition in dermatology

    NASA Astrophysics Data System (ADS)

    Duliu, Alexandru; Gardiazabal, José; Lasser, Tobias; Navab, Nassir

    2015-07-01

    Multispectral image acquisitions are increasingly popular in dermatology, due to their improved spectral resolution which enables better tissue discrimination. Most applications however focus on restricted regions of interest, imaging only small lesions. In this work we present and discuss an imaging framework for high-resolution multispectral imaging on large regions of interest.

  11. Age of Acquisition and Imageability: A Cross-Task Comparison

    ERIC Educational Resources Information Center

    Ploetz, Danielle M.; Yates, Mark

    2016-01-01

    Previous research has reported an imageability effect on visual word recognition. Words that are high in imageability are recognised more rapidly than are those lower in imageability. However, later researchers argued that imageability was confounded with age of acquisition. In the current research, these two factors were manipulated in a…

  12. Accelerated dynamic EPR imaging using fast acquisition and compressive recovery

    NASA Astrophysics Data System (ADS)

    Ahmad, Rizwan; Samouilov, Alexandre; Zweier, Jay L.

    2016-12-01

    Electron paramagnetic resonance (EPR) allows quantitative imaging of tissue redox status, which provides important information about ischemic syndromes, cancer and other pathologies. For continuous wave EPR imaging, however, poor signal-to-noise ratio and low acquisition efficiency limit its ability to image dynamic processes in vivo including tissue redox, where conditions can change rapidly. Here, we present a data acquisition and processing framework that couples fast acquisition with compressive sensing-inspired image recovery to enable EPR-based redox imaging with high spatial and temporal resolutions. The fast acquisition (FA) allows collecting more, albeit noisier, projections in a given scan time. The composite regularization based processing method, called spatio-temporal adaptive recovery (STAR), not only exploits sparsity in multiple representations of the spatio-temporal image but also adaptively adjusts the regularization strength for each representation based on its inherent level of the sparsity. As a result, STAR adjusts to the disparity in the level of sparsity across multiple representations, without introducing any tuning parameter. Our simulation and phantom imaging studies indicate that a combination of fast acquisition and STAR (FASTAR) enables high-fidelity recovery of volumetric image series, with each volumetric image employing less than 10 s of scan. In addition to image fidelity, the time constants derived from FASTAR also match closely to the ground truth even when a small number of projections are used for recovery. This development will enhance the capability of EPR to study fast dynamic processes that cannot be investigated using existing EPR imaging techniques.

  13. Accelerated dynamic EPR imaging using fast acquisition and compressive recovery.

    PubMed

    Ahmad, Rizwan; Samouilov, Alexandre; Zweier, Jay L

    2016-12-01

    Electron paramagnetic resonance (EPR) allows quantitative imaging of tissue redox status, which provides important information about ischemic syndromes, cancer and other pathologies. For continuous wave EPR imaging, however, poor signal-to-noise ratio and low acquisition efficiency limit its ability to image dynamic processes in vivo including tissue redox, where conditions can change rapidly. Here, we present a data acquisition and processing framework that couples fast acquisition with compressive sensing-inspired image recovery to enable EPR-based redox imaging with high spatial and temporal resolutions. The fast acquisition (FA) allows collecting more, albeit noisier, projections in a given scan time. The composite regularization based processing method, called spatio-temporal adaptive recovery (STAR), not only exploits sparsity in multiple representations of the spatio-temporal image but also adaptively adjusts the regularization strength for each representation based on its inherent level of the sparsity. As a result, STAR adjusts to the disparity in the level of sparsity across multiple representations, without introducing any tuning parameter. Our simulation and phantom imaging studies indicate that a combination of fast acquisition and STAR (FASTAR) enables high-fidelity recovery of volumetric image series, with each volumetric image employing less than 10 s of scan. In addition to image fidelity, the time constants derived from FASTAR also match closely to the ground truth even when a small number of projections are used for recovery. This development will enhance the capability of EPR to study fast dynamic processes that cannot be investigated using existing EPR imaging techniques.

  14. An Analysis of Spacecraft Localization from Descent Image Data for Pinpoint Landing on Mars and other Cratered Bodies Data Acquisition

    NASA Technical Reports Server (NTRS)

    Ansar, Adnan; Cheng, Yang

    2005-01-01

    A pinpoint landing capability will be a critical component for many planned NASA missions to Mars and beyond. Implicit in the requirement is the ability to accurately localize the spacecraft with respect to the terrain during descent. In this paper, we present evidence that a vision-based solution using craters as landmarks is both practical and will meet the requirements of next generation missions. Our emphasis in this paper is on the feasibility of such a system in terms of (a) localization accuracy and (b) applicability to Martian terrain. We show that accuracy of well under 100 meters can be expected under suitable conditions. We also present a sensitivity analysis that makes an explicit connection between input data and robustness of our pose estimate. In addition, we present an analysis of the susceptibility of our technique to inherently ambiguous configurations of craters. We show that probability of failure due to such ambiguity is becoming increasingly small.

  15. EBT data acquisition and analysis system

    SciTech Connect

    Burris, R.D.; Greenwood, D.E.; Stanton, J.S.; Geoffroy, K.A.

    1980-10-01

    This document describes the design and implementation of a data acquisition and analysis system for the EBT fusion experiment. The system includes data acquisition on five computers, automatic transmission of that data to a large, central data base, and a powerful data retrieval system. The system is flexible and easy to use, and it provides a fully documented record of the experiments.

  16. Simultaneous acquisition of differing image types

    DOEpatents

    Demos, Stavros G

    2012-10-09

    A system in one embodiment includes an image forming device for forming an image from an area of interest containing different image components; an illumination device for illuminating the area of interest with light containing multiple components; at least one light source coupled to the illumination device, the at least one light source providing light to the illumination device containing different components, each component having distinct spectral characteristics and relative intensity; an image analyzer coupled to the image forming device, the image analyzer decomposing the image formed by the image forming device into multiple component parts based on type of imaging; and multiple image capture devices, each image capture device receiving one of the component parts of the image. A method in one embodiment includes receiving an image from an image forming device; decomposing the image formed by the image forming device into multiple component parts based on type of imaging; receiving the component parts of the image; and outputting image information based on the component parts of the image. Additional systems and methods are presented.

  17. Image acquisition with immersion objective lenses using electrons emitted with several tenths of an electron volt energies: towards high spatial resolution ESCA analysis.

    PubMed

    Bernheim, M

    2006-03-01

    This study aims to evaluate the spatial resolution achievable with photoelectrons in order to perform localised UPS or XPS analyses on various heterogeneous samples. This investigation is intentionally restricted to direct image acquisition by immersion objective lenses, involving electrons ejected with initial energies of several tenths of an electron-volt. In order to characterise the contribution of all optical elements, analytical investigations were associated to numerical simulations based on SIMION 7 software. The acquisition of high-quality images implies a simultaneous reduction in spherical and chromatic aberrations by a narrow aperture stop placed at the output pupil of the objective. With such limitations in useful emission angles, it is shown that monochromatic electron beams build images with a resolution of about 1 nm, especially for the acceleration bias mode where the focussing electrode is biased at a positive high voltage. Even energy dispersed electron beams, limited by a 4 eV band pass spectrometer, can produce images convenient for highly localised ESCA analyses (resolution 3 nm), where the objective lens is associated with an aperture stop of 30 microm in diameter without using acceleration voltages above 5000 V.

  18. The electron spectroscopy for chemical analysis microscopy beamline data acquisition system at ELETTRA

    NASA Astrophysics Data System (ADS)

    Gariazzo, C.; Krempaska, R.; Morrison, G. R.

    1996-07-01

    The electron spectroscopy for chemical analysis (ESCA) microscopy data acquisition system enables the user to control the imaging and spectroscopy modes of operation of the beamline ESCA microscopy at ELETTRA. It allows the user to integrate all experiment, beamline and machine operations in one single environment. The system also provides simple data analysis for both spectra and images data to guide further data acquisition.

  19. Chemical Applications of a Programmable Image Acquisition System

    NASA Astrophysics Data System (ADS)

    Ogren, Paul J.; Henry, Ian; Fletcher, Steven E. S.; Kelly, Ian

    2003-06-01

    Image analysis is widely used in chemistry, both for rapid qualitative evaluations using techniques such as thin layer chromatography (TLC) and for quantitative purposes such as well-plate measurements of analyte concentrations or fragment-size determinations in gel electrophoresis. This paper describes a programmable system for image acquisition and processing that is currently used in the laboratories of our organic and physical chemistry courses. It has also been used in student research projects in analytical chemistry and biochemistry. The potential range of applications is illustrated by brief presentations of four examples: (1) using well-plate optical transmission data to construct a standard concentration absorbance curve; (2) the quantitative analysis of acetaminophen in Tylenol and acetylsalicylic acid in aspirin using TLC with fluorescence detection; (3) the analysis of electrophoresis gels to determine DNA fragment sizes and amounts; and, (4) using color change to follow reaction kinetics. The supplemental material in JCE Online contains information on two additional examples: deconvolution of overlapping bands in protein gel electrophoresis, and the recovery of data from published images or graphs. The JCE Online material also presents additional information on each example, on the system hardware and software, and on the data analysis methodology.

  20. A study on the effect of CT imaging acquisition parameters on lung nodule image interpretation

    NASA Astrophysics Data System (ADS)

    Yu, Shirley J.; Wantroba, Joseph S.; Raicu, Daniela S.; Furst, Jacob D.; Channin, David S.; Armato, Samuel G., III

    2009-02-01

    Most Computer-Aided Diagnosis (CAD) research studies are performed using a single type of Computer Tomography (CT) scanner and therefore, do not take into account the effect of differences in the imaging acquisition scanner parameters. In this paper, we present a study on the effect of the CT parameters on the low-level image features automatically extracted from CT images for lung nodule interpretation. The study is an extension of our previous study where we showed that image features can be used to predict semantic characteristics of lung nodules such as margin, lobulation, spiculation, and texture. Using the Lung Image Data Consortium (LIDC) dataset, we propose to integrate the imaging acquisition parameters with the low-level image features to generate classification models for the nodules' semantic characteristics. Our preliminary results identify seven CT parameters (convolution kernel, reconstruction diameter, exposure, nodule location along the z-axis, distance source to patient, slice thickness, and kVp) as influential in producing classification rules for the LIDC semantic characteristics. Further post-processing analysis, which included running box plots and binning of values, identified four CT parameters: distance source to patient, kVp, nodule location, and rescale intercept. The identification of these parameters will create the premises to normalize the image features across different scanners and, in the long run, generate automatic rules for lung nodules interpretation independently of the CT scanner types.

  1. System of acquisition and processing of images of dynamic speckle

    NASA Astrophysics Data System (ADS)

    Vega, F.; >C Torres,

    2015-01-01

    In this paper we show the design and implementation of a system to capture and analysis of dynamic speckle. The device consists of a USB camera, an isolated system lights for imaging, a laser pointer 633 nm 10 mw as coherent light source, a diffuser and a laptop for processing video. The equipment enables the acquisition and storage of video, also calculated of different descriptors of statistical analysis (vector global accumulation of activity, activity matrix accumulation, cross-correlation vector, autocorrelation coefficient, matrix Fujji etc.). The equipment is designed so that it can be taken directly to the site where the sample for biological study and is currently being used in research projects within the group.

  2. High speed image acquisition system of absolute encoder

    NASA Astrophysics Data System (ADS)

    Liao, Jianxiang; Chen, Xin; Chen, Xindu; Zhang, Fangjian; Wang, Han

    2017-01-01

    Absolute optical encoder as a product of optical, mechanical and electronic integration has been widely used in displacement measuring fields. However, how to improve the measurement velocity and reduce the manufacturing cost of absolute optical encoder is the key problem to be solved. To improve the measurement speed, a novel absolute optical encoder image acquisition system is proposed. The proposed acquisition system includes a linear CCD sensor is applied for capturing coding pattern images, an optical magnifying system is used for enlarging the grating stripes, an analog-digital conversion(ADC) module is used for processing the CCD analogy signal, a field programmable gate array(FPGA) device and other peripherals perform driving task. An absolute position measurement experiment was set up to verify and evaluate the proposed image acquisition system. The experimental result indicates that the proposed absolute optical encoder image acquisition system has the image acquisition speed of more than 9500fp/s with well reliability and lower manufacture cost.

  3. Payload Configurations for Efficient Image Acquisition - Indian Perspective

    NASA Astrophysics Data System (ADS)

    Samudraiah, D. R. M.; Saxena, M.; Paul, S.; Narayanababu, P.; Kuriakose, S.; Kiran Kumar, A. S.

    2014-11-01

    sounder for providing vertical profile of water vapour, temperature, etc. The same system has data relay transponders for acquiring data from weather stations. The payload configurations have gone through significant changes over the years to increase data rate per kilogram of payload. Future Indian remote sensing systems are planned with very high efficient ways of image acquisition. This paper analyses the strides taken by ISRO (Indian Space research Organisation) in achieving high efficiency in remote sensing image data acquisition. Parameters related to efficiency of image data acquisition are defined and a methodology is worked out to compute the same. Some of the Indian payloads are analysed with respect to some of the system/ subsystem parameters that decide the configuration of payload. Based on the analysis, possible configuration approaches that can provide high efficiency are identified. A case study is carried out with improved configuration and the results of efficiency improvements are reported. This methodology may be used for assessing other electro-optical payloads or missions and can be extended to other types of payloads and missions.

  4. A design of camera simulator for photoelectric image acquisition system

    NASA Astrophysics Data System (ADS)

    Cai, Guanghui; Liu, Wen; Zhang, Xin

    2015-02-01

    In the process of developing the photoelectric image acquisition equipment, it needs to verify the function and performance. In order to make the photoelectric device recall the image data formerly in the process of debugging and testing, a design scheme of the camera simulator is presented. In this system, with FPGA as the control core, the image data is saved in NAND flash trough USB2.0 bus. Due to the access rate of the NAND, flash is too slow to meet the requirement of the sytsem, to fix the problem, the pipeline technique and the High-Band-Buses technique are applied in the design to improve the storage rate. It reads image data out from flash in the control logic of FPGA and output separately from three different interface of Camera Link, LVDS and PAL, which can provide image data for photoelectric image acquisition equipment's debugging and algorithm validation. However, because the standard of PAL image resolution is 720*576, the resolution is different between PAL image and input image, so the image can be output after the resolution conversion. The experimental results demonstrate that the camera simulator outputs three format image sequence correctly, which can be captured and displayed by frame gather. And the three-format image data can meet test requirements of the most equipment, shorten debugging time and improve the test efficiency.

  5. Guidelines for dynamic data acquisition and analysis

    NASA Technical Reports Server (NTRS)

    Piersol, Allan G.

    1992-01-01

    The recommendations concerning pyroshock data presented in the final draft of a proposed military handbook on Guidelines for Dynamic Data Acquisition and Analysis are reviewed. The structural responses produced by pyroshocks are considered to be one of the most difficult types of dynamic data to accurately measure and analyze.

  6. Guidelines for dynamic data acquisition and analysis

    NASA Astrophysics Data System (ADS)

    Piersol, Allan G.

    1992-10-01

    The recommendations concerning pyroshock data presented in the final draft of a proposed military handbook on Guidelines for Dynamic Data Acquisition and Analysis are reviewed. The structural responses produced by pyroshocks are considered to be one of the most difficult types of dynamic data to accurately measure and analyze.

  7. Real-Time Protein Crystallization Image Acquisition and Classification System.

    PubMed

    Sigdel, Madhav; Pusey, Marc L; Aygun, Ramazan S

    2013-07-03

    In this paper, we describe the design and implementation of a stand-alone real-time system for protein crystallization image acquisition and classification with a goal to assist crystallographers in scoring crystallization trials. In-house assembled fluorescence microscopy system is built for image acquisition. The images are classified into three categories as non-crystals, likely leads, and crystals. Image classification consists of two main steps - image feature extraction and application of classification based on multilayer perceptron (MLP) neural networks. Our feature extraction involves applying multiple thresholding techniques, identifying high intensity regions (blobs), and generating intensity and blob features to obtain a 45-dimensional feature vector per image. To reduce the risk of missing crystals, we introduce a max-class ensemble classifier which applies multiple classifiers and chooses the highest score (or class). We performed our experiments on 2250 images consisting 67% non-crystal, 18% likely leads, and 15% clear crystal images and tested our results using 10-fold cross validation. Our results demonstrate that the method is very efficient (< 3 seconds to process and classify an image) and has comparatively high accuracy. Our system only misses 1.2% of the crystals (classified as non-crystals) most likely due to low illumination or out of focus image capture and has an overall accuracy of 88%.

  8. Effects of Orientation and Anisometry of Magnetic Resonance Imaging Acquisitions on Diffusion Tensor Imaging and Structural Connectomes

    PubMed Central

    Muñoz-Moreno, Emma; López-Gil, Xavier; Soria, Guadalupe

    2017-01-01

    Diffusion-weighted imaging (DWI) quantifies water molecule diffusion within tissues and is becoming an increasingly used technique. However, it is very challenging as correct quantification depends on many different factors, ranging from acquisition parameters to a long pipeline of image processing. In this work, we investigated the influence of voxel geometry on diffusion analysis, comparing different acquisition orientations as well as isometric and anisometric voxels. Diffusion-weighted images of one rat brain were acquired with four different voxel geometries (one isometric and three anisometric in different directions) and three different encoding orientations (coronal, axial and sagittal). Diffusion tensor scalar measurements, tractography and the brain structural connectome were analyzed for each of the 12 acquisitions. The acquisition direction with respect to the main magnetic field orientation affected the diffusion results. When the acquisition slice-encoding direction was not aligned with the main magnetic field, there were more artifacts and a lower signal-to-noise ratio that led to less anisotropic tensors (lower fractional anisotropic values), producing poorer quality results. The use of anisometric voxels generated statistically significant differences in the values of diffusion metrics in specific regions. It also elicited differences in tract reconstruction and in different graph metric values describing the brain networks. Our results highlight the importance of taking into account the geometric aspects of acquisitions, especially when comparing diffusion data acquired using different geometries. PMID:28118397

  9. Effects of Orientation and Anisometry of Magnetic Resonance Imaging Acquisitions on Diffusion Tensor Imaging and Structural Connectomes.

    PubMed

    Tudela, Raúl; Muñoz-Moreno, Emma; López-Gil, Xavier; Soria, Guadalupe

    2017-01-01

    Diffusion-weighted imaging (DWI) quantifies water molecule diffusion within tissues and is becoming an increasingly used technique. However, it is very challenging as correct quantification depends on many different factors, ranging from acquisition parameters to a long pipeline of image processing. In this work, we investigated the influence of voxel geometry on diffusion analysis, comparing different acquisition orientations as well as isometric and anisometric voxels. Diffusion-weighted images of one rat brain were acquired with four different voxel geometries (one isometric and three anisometric in different directions) and three different encoding orientations (coronal, axial and sagittal). Diffusion tensor scalar measurements, tractography and the brain structural connectome were analyzed for each of the 12 acquisitions. The acquisition direction with respect to the main magnetic field orientation affected the diffusion results. When the acquisition slice-encoding direction was not aligned with the main magnetic field, there were more artifacts and a lower signal-to-noise ratio that led to less anisotropic tensors (lower fractional anisotropic values), producing poorer quality results. The use of anisometric voxels generated statistically significant differences in the values of diffusion metrics in specific regions. It also elicited differences in tract reconstruction and in different graph metric values describing the brain networks. Our results highlight the importance of taking into account the geometric aspects of acquisitions, especially when comparing diffusion data acquired using different geometries.

  10. Automatic image acquisition processor and method

    DOEpatents

    Stone, W.J.

    1984-01-16

    A computerized method and point location system apparatus is disclosed for ascertaining the center of a primitive or fundamental object whose shape and approximate location are known. The technique involves obtaining an image of the object, selecting a trial center, and generating a locus of points having a predetermined relationship with the center. Such a locus of points could include a circle. The number of points overlying the object in each quadrant is obtained and the counts of these points per quadrant are compared. From this comparison, error signals are provided to adjust the relative location of the trial center. This is repeated until the trial center overlies the geometric center within the predefined accuracy limits.

  11. Automatic image acquisition processor and method

    DOEpatents

    Stone, William J.

    1986-01-01

    A computerized method and point location system apparatus is disclosed for ascertaining the center of a primitive or fundamental object whose shape and approximate location are known. The technique involves obtaining an image of the object, selecting a trial center, and generating a locus of points having a predetermined relationship with the center. Such a locus of points could include a circle. The number of points overlying the object in each quadrant is obtained and the counts of these points per quadrant are compared. From this comparison, error signals are provided to adjust the relative location of the trial center. This is repeated until the trial center overlies the geometric center within the predefined accuracy limits.

  12. The solar-image acquisition system at Tor Vergata University.

    NASA Astrophysics Data System (ADS)

    Berrilli, F.; Cantarano, S.; Egidi, A.

    1995-06-01

    Describes an image acquisition system realized as a part of an apparatus built in collaboration with the Arcetri Astrophysical Observatory in Florence designed to record high-spectral-resolution solar images in the visible part of the spectrum. The system is based on a 512×512 Thomson CCD type THX31159 and on a 486 CPU personal computer running under MS-DOS. The electronics for driving the sensor and for the amplification and conditioning of the video signal has been designed and built in the laboratory while the signal A/D conversion and image presentation is performed using commercial boards.

  13. Applications of digital image acquisition in anthropometry

    NASA Technical Reports Server (NTRS)

    Woolford, B.; Lewis, J. L.

    1981-01-01

    A description is given of a video kinesimeter, a device for the automatic real-time collection of kinematic and dynamic data. Based on the detection of a single bright spot by three TV cameras, the system provides automatic real-time recording of three-dimensional position and force data. It comprises three cameras, two incandescent lights, a voltage comparator circuit, a central control unit, and a mass storage device. The control unit determines the signal threshold for each camera before testing, sequences the lights, synchronizes and analyzes the scan voltages from the three cameras, digitizes force from a dynamometer, and codes the data for transmission to a floppy disk for recording. Two of the three cameras face each other along the 'X' axis; the third camera, which faces the center of the line between the first two, defines the 'Y' axis. An image from the 'Y' camera and either 'X' camera is necessary for determining the three-dimensional coordinates of the point.

  14. Image reconstruction for synchronous data acquisition in fluorescence molecular tomography.

    PubMed

    Zhang, Xuanxuan; Liu, Fei; Zuo, Siming; Bai, Jing; Luo, Jianwen

    2015-01-01

    The present full-angle, free-space fluorescence molecular tomography (FMT) system uses a step-by-step strategy to acquire measurements, which consumes time for both the rotation of the object and the integration of the charge-coupled device (CCD) camera. Completing the integration during the rotation is a more time-efficient strategy called synchronous data acquisition. However, the positions of sources and detectors in this strategy are not stationary, which is not taken into account in the conventional reconstruction algorithm. In this paper we propose a reconstruction algorithm based on the finite element method (FEM) to overcome this problem. Phantom experiments were carried out to validate the performance of the algorithm. The results show that, compared with the conventional reconstruction algorithm used in the step-by-step data acquisition strategy, the proposed algorithm can reconstruct images with more accurate location data and lower relative errors when used with the synchronous data acquisition strategy.

  15. A Joint Acquisition-Estimation Framework for MR Phase Imaging

    PubMed Central

    Dagher, Joseph

    2015-01-01

    Measuring the phase of the MR signal is faced with fundamental challenges such as phase aliasing, noise and unknown offsets of the coil array. There is a paucity of acquisition, reconstruction and estimation methods that rigorously address these challenges. This reduces the reliability of information processing in phase domain. We propose a joint acquisition-processing framework that addresses the challenges of MR phase imaging using a rigorous theoretical treatment. Our proposed solution acquires the multi-coil complex data without any increase in acquisition time. Our corresponding estimation algorithm is applied optimally voxel-per-voxel. Results show that our framework achieves performance gains up to an order of magnitude compared to existing methods. PMID:26221666

  16. Rock fracture image acquisition with both visible and ultraviolet illuminations

    NASA Astrophysics Data System (ADS)

    Wang, Weixing; Hakami, Eva

    2006-02-01

    Swedish Nuclear Fuel and Waste Management Company (SKB) have identified the need for a better understanding of radionuclide transport and retention processes in fractured rock since 1994. In the study, the first hard problem is to obtain rock fracture images of a good quality, since rock surface is very rough, and composed of complicated and multiple fractures, as a result, image acquisition is the first important. As a cooperation project between Sweden and China, we sampled a number of rock specimens for analyzing rock fracture network by visible and ultraviolet image technique, in the field. The samples are resin injected, in which way; opened fractures can be seen clearly by means of UV light illumination, and the rock surface information can be obtained by using visible optical illumination. We used different digital cameras and microscope to take images by two illuminations. From the same samples; we found that UV illumination image gives the clear information of fracture opening or closing, and the visible optical illumination gives the information of the rock surface (e.g. filling materials inside of fractures). By applying this technique, the minimum width of rock fracture 0.01 mm can be analyzed. This paper presents: (1) Rock fracture image acquiring techniques; (2) Rock fracture image acquisition by using UV light illumination and visible optical illumination; and (3) Conclusions. The studied method can be used both in the field and a laboratory.

  17. Hyperspectral image analysis. A tutorial.

    PubMed

    Amigo, José Manuel; Babamoradi, Hamid; Elcoroaristizabal, Saioa

    2015-10-08

    This tutorial aims at providing guidelines and practical tools to assist with the analysis of hyperspectral images. Topics like hyperspectral image acquisition, image pre-processing, multivariate exploratory analysis, hyperspectral image resolution, classification and final digital image processing will be exposed, and some guidelines given and discussed. Due to the broad character of current applications and the vast number of multivariate methods available, this paper has focused on an industrial chemical framework to explain, in a step-wise manner, how to develop a classification methodology to differentiate between several types of plastics by using Near infrared hyperspectral imaging and Partial Least Squares - Discriminant Analysis. Thus, the reader is guided through every single step and oriented in order to adapt those strategies to the user's case.

  18. Reflections on ultrasound image analysis.

    PubMed

    Alison Noble, J

    2016-10-01

    Ultrasound (US) image analysis has advanced considerably in twenty years. Progress in ultrasound image analysis has always been fundamental to the advancement of image-guided interventions research due to the real-time acquisition capability of ultrasound and this has remained true over the two decades. But in quantitative ultrasound image analysis - which takes US images and turns them into more meaningful clinical information - thinking has perhaps more fundamentally changed. From roots as a poor cousin to Computed Tomography (CT) and Magnetic Resonance (MR) image analysis, both of which have richer anatomical definition and thus were better suited to the earlier eras of medical image analysis which were dominated by model-based methods, ultrasound image analysis has now entered an exciting new era, assisted by advances in machine learning and the growing clinical and commercial interest in employing low-cost portable ultrasound devices outside traditional hospital-based clinical settings. This short article provides a perspective on this change, and highlights some challenges ahead and potential opportunities in ultrasound image analysis which may both have high impact on healthcare delivery worldwide in the future but may also, perhaps, take the subject further away from CT and MR image analysis research with time.

  19. Acquisition and Post-Processing of Immunohistochemical Images.

    PubMed

    Sedgewick, Jerry

    2017-01-01

    Augmentation of digital images is almost always a necessity in order to obtain a reproduction that matches the appearance of the original. However, that augmentation can mislead if it is done incorrectly and not within reasonable limits. When procedures are in place for insuring that originals are archived, and image manipulation steps reported, scientists not only follow good laboratory practices, but avoid ethical issues associated with post processing, and protect their labs from any future allegations of scientific misconduct. Also, when procedures are in place for correct acquisition of images, the extent of post processing is minimized or eliminated. These procedures include white balancing (for brightfield images), keeping tonal values within the dynamic range of the detector, frame averaging to eliminate noise (typically in fluorescence imaging), use of the highest bit depth when a choice is available, flatfield correction, and archiving of the image in a non-lossy format (not JPEG).When post-processing is necessary, the commonly used applications for correction include Photoshop, and ImageJ, but a free program (GIMP) can also be used. Corrections to images include scaling the bit depth to higher and lower ranges, removing color casts from brightfield images, setting brightness and contrast, reducing color noise, reducing "grainy" noise, conversion of pure colors to grayscale, conversion of grayscale to colors typically used in fluorescence imaging, correction of uneven illumination (flatfield correction), merging color images (fluorescence), and extending the depth of focus. These corrections are explained in step-by-step procedures in the chapter that follows.

  20. Data acquisition and analysis on a Macintosh

    NASA Technical Reports Server (NTRS)

    Watts, Michael E.; St. Jean, Megan M.

    1991-01-01

    The introduction of inexpensive analog-to-digital boards for the Macintosh opens the way for its use in areas that have previously been filled by either specialized, dedicated or more expensive mainframe based systems. Two such Macintosh-based systems are the Acoustic Laboratory Data Acquisition System (ALDAS) and the Jet Calibration and Hover Test Facility (JCAHT) data acquisition system. ALDAS provides an inexpensive, transportable means to digitize four channels at up to 50,000 samples per second and analyze this data. The ALDAS software package was written for use with rotorcraft acoustics and performs automatic acoustic calibration of channels, data display, and various types of data analysis. The program can use data obtained either from internal analog-to-digital conversion or discrete external data imported in ASCII format. All aspects of ALDAS can be improved as new hardware becomes available and new features are introduced into the code. The JCAHT data acquisition system was built as not only an analysis program but also to act as the online safety monitoring system. This paper will provide an overview of these systems.

  1. Smartphone Image Acquisition During Postmortem Monocular Indirect Ophthalmoscopy.

    PubMed

    Lantz, Patrick E; Schoppe, Candace H; Thibault, Kirk L; Porter, William T

    2016-01-01

    The medical usefulness of smartphones continues to evolve as third-party applications exploit and expand on the smartphones' interface and capabilities. This technical report describes smartphone still-image capture techniques and video-sequence recording capabilities during postmortem monocular indirect ophthalmoscopy. Using these devices and techniques, practitioners can create photographic documentation of fundal findings, clinically and at autopsy, without the expense of a retinal camera. Smartphone image acquisition of fundal abnormalities can promote ophthalmological telemedicine--especially in regions or countries with limited resources--and facilitate prompt, accurate, and unbiased documentation of retinal hemorrhages in infants and young children.

  2. Isothermal thermogravimetric data acquisition analysis system

    NASA Technical Reports Server (NTRS)

    Cooper, Kenneth, Jr.

    1991-01-01

    The description of an Isothermal Thermogravimetric Analysis (TGA) Data Acquisition System is presented. The system consists of software and hardware to perform a wide variety of TGA experiments. The software is written in ANSI C using Borland's Turbo C++. The hardware consists of a 486/25 MHz machine with a Capital Equipment Corp. IEEE488 interface card. The interface is to a Hewlett Packard 3497A data acquisition system using two analog input cards and a digital actuator card. The system provides for 16 TGA rigs with weight and temperature measurements from each rig. Data collection is conducted in three phases. Acquisition is done at a rapid rate during initial startup, at a slower rate during extended data collection periods, and finally at a fast rate during shutdown. Parameters controlling the rate and duration of each phase are user programmable. Furnace control (raising and lowering) is also programmable. Provision is made for automatic restart in the event of power failure or other abnormal terminations. Initial trial runs were conducted to show system stability.

  3. Optical Nanoscopy: From Acquisition to Analysis

    PubMed Central

    Gould, Travis J.; Hess, Samuel T.; Bewersdorf, Joerg

    2013-01-01

    Recent advances in far-field microscopy have demonstrated that fluorescence imaging is possible at resolutions well below the long-standing diffraction limit. By exploiting photophysical properties of fluorescent probe molecules, this new class of methods yields a resolving power that is fundamentally diffraction unlimited. Although these methods are becoming more widely used in biological imaging, they must be complemented by suitable data analysis approaches if their potential is to be fully realized. Here we review the basic principles of diffraction-unlimited microscopy and how these principles influence the selection of available algorithms for data analysis. Furthermore, we provide an overview of existing analysis strategies and discuss their application. PMID:22559319

  4. Digital acquisition system for high-speed 3-D imaging

    NASA Astrophysics Data System (ADS)

    Yafuso, Eiji

    1997-11-01

    High-speed digital three-dimensional (3-D) imagery is possible using multiple independent charge-coupled device (CCD) cameras with sequentially triggered acquisition and individual field storage capability. The system described here utilizes sixteen independent cameras, providing versatility in configuration and image acquisition. By aligning the cameras in nearly coincident lines-of-sight, a sixteen frame two-dimensional (2-D) sequence can be captured. The delays can be individually adjusted lo yield a greater number of acquired frames during the more rapid segments of the event. Additionally, individual integration periods may be adjusted to ensure adequate radiometric response while minimizing image blur. An alternative alignment and triggering scheme arranges the cameras into two angularly separated banks of eight cameras each. By simultaneously triggering correlated stereo pairs, an eight-frame sequence of stereo images may be captured. In the first alignment scheme the camera lines-of-sight cannot be made precisely coincident. Thus representation of the data as a monocular sequence introduces the issue of independent camera coordinate registration with the real scene. This issue arises more significantly using the stereo pair method to reconstruct quantitative 3-D spatial information of the event as a function of time. The principal development here will be the derivation and evaluation of a solution transform and its inverse for the digital data which will yield a 3-D spatial mapping as a function of time.

  5. PET/CT for radiotherapy: image acquisition and data processing.

    PubMed

    Bettinardi, V; Picchio, M; Di Muzio, N; Gianolli, L; Messa, C; Gilardi, M C

    2010-10-01

    This paper focuses on acquisition and processing methods in positron emission tomography/computed tomography (PET/CT) for radiotherapy (RT) applications. The recent technological evolutions of PET/CT systems are described. Particular emphasis is dedicated to the tools needed for the patient positioning and immobilization, to be used in PET/CT studies as well as during RT treatment sessions. The effect of organ and lesion motion due to patient's respiration on PET/CT imaging is discussed. Breathing protocols proposed to minimize PET/CT spatial mismatches in relation to respiratory movements are illustrated. The respiratory gated (RG) 4D-PET/CT techniques, developed to measure and compensate for organ and lesion motion, are then introduced. Finally a description is provided of different acquisition and data processing techniques, implemented with the aim at improving: i) image quality and quantitative accuracy of PET images, and ii) target volume definition and treatment planning in RT, by using specific and personalised motion information.

  6. Radiologist and automated image analysis

    NASA Astrophysics Data System (ADS)

    Krupinski, Elizabeth A.

    1999-07-01

    Significant advances are being made in the area of automated medical image analysis. Part of the progress is due to the general advances being made in the types of algorithms used to process images and perform various detection and recognition tasks. A more important reason for this growth in medical image analysis processes, may be due however to a very different reason. The use of computer workstations, digital image acquisition technologies and the use of CRT monitors for display of medical images for primary diagnostic reading is becoming more prevalent in radiology departments around the world. With the advance in computer- based displays, however, has come the realization that displaying images on a CRT monitor is not the same as displaying film on a viewbox. There are perceptual, cognitive and ergonomic issues that must be considered if radiologists are to accept this change in technology and display. The bottom line is that radiologists' performance must be evaluated with these new technologies and image analysis techniques in order to verify that diagnostic performance is at least as good with these new technologies and image analysis procedures as with film-based displays. The goal of this paper is to address some of the perceptual, cognitive and ergonomic issues associated with reading radiographic images from digital displays.

  7. Commonality analysis as a knowledge acquisition problem

    NASA Technical Reports Server (NTRS)

    Yeager, Dorian P.

    1987-01-01

    Commonality analysis is a systematic attempt to reduce costs in a large scale engineering project by discontinuing development of certain components during the design phase. Each discontinued component is replaced by another component that has sufficient functionality to be considered an appropriate substitute. The replacement strategy is driven by economic considerations. The System Commonality Analysis Tool (SCAT) is based on an oversimplified model of the problem and incorporates no knowledge acquisition component. In fact, the process of arriving at a compromise between functionality and economy is quite complex, with many opportunities for the application of expert knowledge. Such knowledge is of two types: general knowledge expressible as heuristics or mathematical laws potentially applicable to any set of components, and specific knowledge about the way in which elements of a given set of components interrelate. Examples of both types of knowledge are presented, and a framework is proposed for integrating the knowledge into a more general and useable tool.

  8. Status of RAISE, the Rapid Acquisition Imaging Spectrograph Experiment

    NASA Astrophysics Data System (ADS)

    Laurent, Glenn T.; Hassler, D. M.; DeForest, C.; Ayres, T. R.; Davis, M.; De Pontieu, B.; Schuehle, U.; Warren, H.

    2013-07-01

    The Rapid Acquisition Imaging Spectrograph Experiment (RAISE) sounding rocket payload is a high speed scanning-slit imaging spectrograph designed to observe the dynamics and heating of the solar chromosphere and corona on time scales as short as 100 ms, with 1 arcsec spatial resolution and a velocity sensitivity of 1-2 km/s. The instrument is based on a new class of UV/EUV imaging spectrometers that use only two reflections to provide quasi-stigmatic performance simultaneously over multiple wavelengths and spatial fields. The design uses an off-axis parabolic telescope mirror to form a real image of the sun on the spectrometer entrance aperture. A slit then selects a portion of the solar image, passing its light onto a near-normal incidence toroidal grating, which re-images the spectrally dispersed radiation onto two array detectors. Two full spectral passbands over the same one-dimensional spatial field are recorded simultaneously with no scanning of the detectors or grating. The two different spectral bands (1st-order 1205-1243Å and 1526-1564Å) are imaged onto two intensified Active Pixel Sensor (APS) detectors whose focal planes are individually adjusted for optimized performance. The telescope and grating are coated with B4C to enhance short wavelength (2nd order) reflectance, enabling the instrument to record the brightest lines between 602-622Å and 761-780Å at the same time. RAISE reads out the full field of both detectors at 5-10 Hz, allowing us to record over 1,500 complete spectral observations in a single 5-minute rocket flight, opening up a new domain of high time resolution spectral imaging and spectroscopy. We present an overview of the project, a summary of the maiden flight results, and an update on instrument status.Abstract (2,250 Maximum Characters): The Rapid Acquisition Imaging Spectrograph Experiment (RAISE) sounding rocket payload is a high speed scanning-slit imaging spectrograph designed to observe the dynamics and heating of the solar

  9. Simultaneous acquisition of spatial harmonics (SMASH): fast imaging with radiofrequency coil arrays.

    PubMed

    Sodickson, D K; Manning, W J

    1997-10-01

    SiMultaneous Acquisition of Spatial Harmonics (SMASH) is a new fast-imaging technique that increases MR image acquisition speed by an integer factor over existing fast-imaging methods, without significant sacrifices in spatial resolution or signal-to-noise ratio. Image acquisition time is reduced by exploiting spatial information inherent in the geometry of a surface coil array to substitute for some of the phase encoding usually produced by magnetic field gradients. This allows for partially parallel image acquisitions using many of the existing fast-imaging sequences. Unlike the data combination algorithms of prior proposals for parallel imaging, SMASH reconstruction involves a small set of MR signal combinations prior to Fourier transformation, which can be advantageous for artifact handling and practical implementation. A twofold savings in image acquisition time is demonstrated here using commercial phased array coils on two different MR-imaging systems. Larger time savings factors can be expected for appropriate coil designs.

  10. Data acquisition system for harmonic motion microwave Doppler imaging.

    PubMed

    Tafreshi, Azadeh Kamali; Karadaş, Mürsel; Top, Can Barış; Gençer, Nevzat Güneri

    2014-01-01

    Harmonic Motion Microwave Doppler Imaging (HMMDI) is a hybrid method proposed for breast tumor detection, which images the coupled dielectric and elastic properties of the tissue. In this paper, the performance of a data acquisition system for HMMDI method is evaluated on breast phantom materials. A breast fat phantom including fibro-glandular and tumor phantom regions is produced. The phantom is excited using a focused ultrasound probe and a microwave transmitter. The received microwave signal level is measured on three different points inside the phantom (fat, fibro-glandular, and tumor regions). The experimental results using the designed homodyne receiver proved the effectiveness of the proposed setup. In tumor phantom region, the signal level decreased about 3 dB compared to the signal level obtained from the fibro-glandular phantom area, whereas this signal was about 4 dB higher than the received signal from the fat phantom.

  11. Least Squares Time-Series Synchronization in Image Acquisition Systems.

    PubMed

    Piazzo, Lorenzo; Raguso, Maria Carmela; Calzoletti, Luca; Seu, Roberto; Altieri, Bruno

    2016-07-18

    We consider an acquisition system constituted by an array of sensors scanning an image. Each sensor produces a sequence of readouts, called a time-series. In this framework, we discuss the image estimation problem when the time-series are affected by noise and by a time shift. In particular, we introduce an appropriate data model and consider the Least Squares (LS) estimate, showing that it has no closed form. However, the LS problem has a structure that can be exploited to simplify the solution. In particular, based on two known techniques, namely Separable Nonlinear Least Squares (SNLS) and Alternating Least Squares (ALS), we propose and analyze several practical estimation methods. As an additional contribution, we discuss the application of these methods to the data of the Photodetector Array Camera and Spectrometer (PACS), which is an infrared photometer onboard the Herschel satellite. In this context, we investigate the accuracy and the computational complexity of the methods, using both true and simulated data.

  12. RAISE (Rapid Acquisition Imaging Spectrograph Experiment): Results and Instrument Status

    NASA Astrophysics Data System (ADS)

    Laurent, Glenn T.; Hassler, Donald; DeForest, Craig; Ayres, Tom; Davis, Michael; DePontieu, Bart; Diller, Jed; Graham, Roy; Schule, Udo; Warren, Harry

    2015-04-01

    We present initial results from the successful November 2014 launch of the RAISE (Rapid Acquisition Imaging Spectrograph Experiment) sounding rocket program, including intensity maps, high-speed spectroheliograms and dopplergrams, as well as an update on instrument status. The RAISE sounding rocket payload is the fastest high-speed scanning-slit imaging spectrograph flown to date and is designed to observe the dynamics and heating of the solar chromosphere and corona on time scales as short as 100-200ms, with arcsecond spatial resolution and a velocity sensitivity of 1-2 km/s. The instrument is based on a class of UV/EUV imaging spectrometers that use only two reflections to provide quasi-stigmatic performance simultaneously over multiple wavelengths and spatial fields. The design uses an off-axis parabolic telescope mirror to form a real image of the sun on the spectrometer entrance aperture. A slit then selects a portion of the solar image, passing its light onto a near-normal incidence toroidal grating, which re-images the spectrally dispersed radiation onto two array detectors. Two full spectral passbands over the same one-dimensional spatial field are recorded simultaneously with no scanning of the detectors or grating. The two different spectral bands (1st-order 1205-1243Å and 1526-1564Å) are imaged onto two intensified Active Pixel Sensor (APS) detectors whose focal planes are individually adjusted for optimized performance. RAISE reads out the full field of both detectors at 5-10 Hz, allowing us to record over 1,500 complete spectral observations in a single 5-minute rocket flight, opening up a new domain of high time resolution spectral imaging and spectroscopy. RAISE is designed to study small-scale multithermal dynamics in active region (AR) loops, explore the strength, spectrum and location of high frequency waves in the solar atmosphere, and investigate the nature of transient brightenings in the chromospheric network.

  13. Motion-gated acquisition for in vivo optical imaging

    PubMed Central

    Gioux, Sylvain; Ashitate, Yoshitomo; Hutteman, Merlijn; Frangioni, John V.

    2009-01-01

    Wide-field continuous wave fluorescence imaging, fluorescence lifetime imaging, frequency domain photon migration, and spatially modulated imaging have the potential to provide quantitative measurements in vivo. However, most of these techniques have not yet been successfully translated to the clinic due to challenging environmental constraints. In many circumstances, cardiac and respiratory motion greatly impair image quality and∕or quantitative processing. To address this fundamental problem, we have developed a low-cost, field-programmable gate array–based, hardware-only gating device that delivers a phase-locked acquisition window of arbitrary delay and width that is derived from an unlimited number of pseudo-periodic and nonperiodic input signals. All device features can be controlled manually or via USB serial commands. The working range of the device spans the extremes of mouse electrocardiogram (1000 beats per minute) to human respiration (4 breaths per minute), with timing resolution ⩽0.06%, and jitter ⩽0.008%, of the input signal period. We demonstrate the performance of the gating device, including dramatic improvements in quantitative measurements, in vitro using a motion simulator and in vivo using near-infrared fluorescence angiography of beating pig heart. This gating device should help to enable the clinical translation of promising new optical imaging technologies. PMID:20059276

  14. Reducing respiratory effect in motion correction for EPI images with sequential slice acquisition order.

    PubMed

    Cheng, Hu; Puce, Aina

    2014-04-30

    Motion correction is critical for data analysis of fMRI time series. Most motion correction algorithms treat the head as a rigid body. Respiration of the subject, however, can alter the static magnetic field in the head and result in motion-like slice shifts for echo planar imaging (EPI). The delay of acquisition between slices causes a phase difference in respiration so that the shifts vary with slice positions. To characterize the effect of respiration on motion correction, we acquired fast sampled fMRI data using multi-band EPI and then simulated different acquisition schemes. Our results indicated that respiration introduces additional noise after motion correction. The signal variation between volumes after motion correction increases when the effective TR increases from 675ms to 2025ms. This problem can be corrected if slices are acquired sequentially. For EPI with a sequential acquisition scheme, we propose to divide the image volumes into several segments so that slices within each segment are acquired close in time and then perform motion correction on these segments separately. We demonstrated that the temporal signal-to-noise ratio (TSNR) was increased when the motion correction was performed on the segments separately rather than on the whole image. This enhancement of TSNR was not evenly distributed across the segments and was not observed for interleaved acquisition. The level of increase was higher for superior slices. On superior slices the percentage of TSNR gain was comparable to that using image based retrospective correction for respiratory noise. Our results suggest that separate motion correction on segments is highly recommended for sequential acquisition schemes, at least for slices distal to the chest.

  15. The Rapid Acquisition Imaging Spectrograph Experiment (RAISE) Sounding Rocket Investigation

    NASA Astrophysics Data System (ADS)

    Laurent, Glenn T.; Hassler, Donald M.; Deforest, Craig; Slater, David D.; Thomas, Roger J.; Ayres, Thomas; Davis, Michael; de Pontieu, Bart; Diller, Jed; Graham, Roy; Michaelis, Harald; Schuele, Udo; Warren, Harry

    2016-03-01

    We present a summary of the solar observing Rapid Acquisition Imaging Spectrograph Experiment (RAISE) sounding rocket program including an overview of the design and calibration of the instrument, flight performance, and preliminary chromospheric results from the successful November 2014 launch of the RAISE instrument. The RAISE sounding rocket payload is the fastest scanning-slit solar ultraviolet imaging spectrograph flown to date. RAISE is designed to observe the dynamics and heating of the solar chromosphere and corona on time scales as short as 100-200ms, with arcsecond spatial resolution and a velocity sensitivity of 1-2km/s. Two full spectral passbands over the same one-dimensional spatial field are recorded simultaneously with no scanning of the detectors or grating. The two different spectral bands (first-order 1205-1251Å and 1524-1569Å) are imaged onto two intensified Active Pixel Sensor (APS) detectors whose focal planes are individually adjusted for optimized performance. RAISE reads out the full field of both detectors at 5-10Hz, recording up to 1800 complete spectra (per detector) in a single 6-min rocket flight. This opens up a new domain of high time resolution spectral imaging and spectroscopy. RAISE is designed to observe small-scale multithermal dynamics in Active Region (AR) and quiet Sun loops, identify the strength, spectrum and location of high frequency waves in the solar atmosphere, and determine the nature of energy release in the chromospheric network.

  16. The selection of field acquisition parameters for dispersion images from multichannel surface wave data

    USGS Publications Warehouse

    Zhang, S.X.; Chan, L.S.; Xia, J.

    2004-01-01

    The accuracy and resolution of surface wave dispersion results depend on the parameters used for acquiring data in the field. The optimized field parameters for acquiring multichannel analysis of surface wave (MASW) dispersion images can be determined if preliminary information on the phase velocity range and interface depth is available. In a case study on a fill slope in Hong Kong, the optimal acquisition parameters were first determined from a preliminary seismic survey prior to a MASW survey. Field tests using different sets of receiver distances and array lengths showed that the most consistent and useful dispersion images were obtained from the optimal acquisition parameters predicted. The inverted S-wave velocities from the dispersion curve obtained at the optimal offset distance range also agreed with those obtained by using direct refraction survey.

  17. Advances in GPR data acquisition and analysis for archaeology

    NASA Astrophysics Data System (ADS)

    Zhao, Wenke; Tian, Gang; Forte, Emanuele; Pipan, Michele; Wang, Yimin; Li, Xuejing; Shi, Zhanjie; Liu, Haiyan

    2015-07-01

    The primary objective of this study is to evaluate the applicability and the effectiveness of ground-penetrating radar (GPR) to identify a thin burnt soil layer, buried more than 2 m below the topographic surface at the Liangzhu Site, in Southeastern China. The site was chosen for its relatively challenging conditions of GPR techniques due to electrical conductivity and to the presence of peach tree roots that produced scattering. We completed the data acquisition by using 100 and 200 MHz antennas in TE and TM broadside and cross-polarized configurations. In the data processing and interpretation phase, we used GPR attribute analysis, including instantaneous phase and geometrical attributes. Validation analysis ground-truthing performed after the geophysical surveys, validated the GPR imaging, confirmed the electrical conductivity and relative dielectric permittivity (RDP) measurements performed at different depths, and allowed a reliable quantitative correlation between GPR results and subsurface physical properties. The research demonstrates that multiple antenna configurations in GPR data acquisition combined with attribute analysis can enhance the ability to characterize prehistoric archaeological remains even in complex subsurface conditions.

  18. Probability of acquisition of three-dimensional imaging laser radar

    NASA Astrophysics Data System (ADS)

    Dong, Li-jun; Zhu, Shao-lan; Sun, Chuan-dong; Gao, Cun-xiao; Song, Zhi-yuan

    2011-06-01

    Three-dimensional imaging laser radar (3-D ladar) is widely used in area of modern military, scientific research, agriculture and industry. Because of its many features such as angle-angle-range capturing, high resolution, anti-jamming ability and no multipath effect ,but it has to scan for target searching, acquiring and tracking. This paper presents a novel probability model of target acquiring which provides a theoretical basis for optimizing the scanning mechanism. The model combines space and time, target moving velocity and ladar scanning velocity together. Then the optimum scanning mechanism to obtain the maximum probability of acquisition and associated with different targets can be gained. The result shows that this model provides a method to optimize parameter for designing of the scanner.

  19. Planning applications in image analysis

    NASA Technical Reports Server (NTRS)

    Boddy, Mark; White, Jim; Goldman, Robert; Short, Nick, Jr.

    1994-01-01

    We describe two interim results from an ongoing effort to automate the acquisition, analysis, archiving, and distribution of satellite earth science data. Both results are applications of Artificial Intelligence planning research to the automatic generation of processing steps for image analysis tasks. First, we have constructed a linear conditional planner (CPed), used to generate conditional processing plans. Second, we have extended an existing hierarchical planning system to make use of durations, resources, and deadlines, thus supporting the automatic generation of processing steps in time and resource-constrained environments.

  20. Efficient image acquisition design for a cancer detection system

    NASA Astrophysics Data System (ADS)

    Nguyen, Dung; Roehrig, Hans; Borders, Marisa H.; Fitzpatrick, Kimberly A.; Roveda, Janet

    2013-09-01

    Modern imaging modalities, such as Computed Tomography (CT), Digital Breast Tomosynthesis (DBT) or Magnetic Resonance Tomography (MRT) are able to acquire volumetric images with an isotropic resolution in micrometer (um) or millimeter (mm) range. When used in interactive telemedicine applications, these raw images need a huge storage unit, thereby necessitating the use of high bandwidth data communication link. To reduce the cost of transmission and enable archiving, especially for medical applications, image compression is performed. Recent advances in compression algorithms have resulted in a vast array of data compression techniques, but because of the characteristics of these images, there are challenges to overcome to transmit these images efficiently. In addition, the recent studies raise the low dose mammography risk on high risk patient. Our preliminary studies indicate that by bringing the compression before the analog-to-digital conversion (ADC) stage is more efficient than other compression techniques after the ADC. The linearity characteristic of the compressed sensing and ability to perform the digital signal processing (DSP) during data conversion open up a new area of research regarding the roles of sparsity in medical image registration, medical image analysis (for example, automatic image processing algorithm to efficiently extract the relevant information for the clinician), further Xray dose reduction for mammography, and contrast enhancement.

  1. Image Analysis and Modeling

    DTIC Science & Technology

    1976-03-01

    This report summarizes the results of the research program on Image Analysis and Modeling supported by the Defense Advanced Research Projects Agency...The objective is to achieve a better understanding of image structure and to use this knowledge to develop improved image models for use in image ... analysis and processing tasks such as information extraction, image enhancement and restoration, and coding. The ultimate objective of this research is

  2. Democratizing an electroluminescence imaging apparatus and analytics project for widespread data acquisition in photovoltaic materials

    NASA Astrophysics Data System (ADS)

    Fada, Justin S.; Wheeler, Nicholas R.; Zabiyaka, Davis; Goel, Nikhil; Peshek, Timothy J.; French, Roger H.

    2016-08-01

    We present a description of an electroluminescence (EL) apparatus, easily sourced from commercially available components, with a quantitative image processing platform that demonstrates feasibility for the widespread utility of EL imaging as a characterization tool. We validated our system using a Gage R&R analysis to find a variance contribution by the measurement system of 80.56%, which is typically unacceptable, but through quantitative image processing and development of correction factors a variance contribution by the measurement system of 2.41% was obtained. We further validated the system by quantifying the signal-to-noise ratio (SNR) and found values consistent with other systems published in the literature, at SNR values of 10-100, albeit at exposure times of greater than 1 s compared to 10 ms for other systems. This SNR value range is acceptable for image feature recognition, providing the opportunity for widespread data acquisition and large scale data analytics of photovoltaics.

  3. Democratizing an electroluminescence imaging apparatus and analytics project for widespread data acquisition in photovoltaic materials.

    PubMed

    Fada, Justin S; Wheeler, Nicholas R; Zabiyaka, Davis; Goel, Nikhil; Peshek, Timothy J; French, Roger H

    2016-08-01

    We present a description of an electroluminescence (EL) apparatus, easily sourced from commercially available components, with a quantitative image processing platform that demonstrates feasibility for the widespread utility of EL imaging as a characterization tool. We validated our system using a Gage R&R analysis to find a variance contribution by the measurement system of 80.56%, which is typically unacceptable, but through quantitative image processing and development of correction factors a variance contribution by the measurement system of 2.41% was obtained. We further validated the system by quantifying the signal-to-noise ratio (SNR) and found values consistent with other systems published in the literature, at SNR values of 10-100, albeit at exposure times of greater than 1 s compared to 10 ms for other systems. This SNR value range is acceptable for image feature recognition, providing the opportunity for widespread data acquisition and large scale data analytics of photovoltaics.

  4. A Parallel Distributed-Memory Particle Method Enables Acquisition-Rate Segmentation of Large Fluorescence Microscopy Images

    PubMed Central

    Afshar, Yaser; Sbalzarini, Ivo F.

    2016-01-01

    Modern fluorescence microscopy modalities, such as light-sheet microscopy, are capable of acquiring large three-dimensional images at high data rate. This creates a bottleneck in computational processing and analysis of the acquired images, as the rate of acquisition outpaces the speed of processing. Moreover, images can be so large that they do not fit the main memory of a single computer. We address both issues by developing a distributed parallel algorithm for segmentation of large fluorescence microscopy images. The method is based on the versatile Discrete Region Competition algorithm, which has previously proven useful in microscopy image segmentation. The present distributed implementation decomposes the input image into smaller sub-images that are distributed across multiple computers. Using network communication, the computers orchestrate the collectively solving of the global segmentation problem. This not only enables segmentation of large images (we test images of up to 1010 pixels), but also accelerates segmentation to match the time scale of image acquisition. Such acquisition-rate image segmentation is a prerequisite for the smart microscopes of the future and enables online data compression and interactive experiments. PMID:27046144

  5. Collaborative Research and Development (CR&D) III Task Order 0090: Image Processing Framework: From Acquisition and Analysis to Archival Storage

    DTIC Science & Technology

    2013-05-01

    loy in three and two dimensions using the Saltykov method .’ ’ Scripta Materialia. Acta Materialia Inc., 66(8), 554-557. 15 Approved for public...images and data. 15. SUBJECT TERMS DREAM3D, Finite Element Method (FEM), Integrated Computational Materials Science (ICME), Graphical Interface...Finite E lement Method algorithms. data acqui sition sensitivity studi es and linking material properties to material structure. T he software is s

  6. Multi-channel pre-beamformed data acquisition system for research on advanced ultrasound imaging methods.

    PubMed

    Cheung, Chris C P; Yu, Alfred C H; Salimi, Nazila; Yiu, Billy Y S; Tsang, Ivan K H; Kerby, Benjamin; Azar, Reza Zahiri; Dickie, Kris

    2012-02-01

    The lack of open access to the pre-beamformed data of an ultrasound scanner has limited the research of novel imaging methods to a few privileged laboratories. To address this need, we have developed a pre-beamformed data acquisition (DAQ) system that can collect data over 128 array elements in parallel from the Ultrasonix series of research-purpose ultrasound scanners. Our DAQ system comprises three system-level blocks: 1) a connector board that interfaces with the array probe and the scanner through a probe connector port; 2) a main board that triggers DAQ and controls data transfer to a computer; and 3) four receiver boards that are each responsible for acquiring 32 channels of digitized raw data and storing them to the on-board memory. This system can acquire pre-beamformed data with 12-bit resolution when using a 40-MHz sampling rate. It houses a 16 GB RAM buffer that is sufficient to store 128 channels of pre-beamformed data for 8000 to 25 000 transmit firings, depending on imaging depth; corresponding to nearly a 2-s period in typical imaging setups. Following the acquisition, the data can be transferred through a USB 2.0 link to a computer for offline processing and analysis. To evaluate the feasibility of using the DAQ system for advanced imaging research, two proof-of-concept investigations have been conducted on beamforming and plane-wave B-flow imaging. Results show that adaptive beamforming algorithms such as the minimum variance approach can generate sharper images of a wire cross-section whose diameter is equal to the imaging wavelength (150 μm in our example). Also, planewave B-flow imaging can provide more consistent visualization of blood speckle movement given the higher temporal resolution of this imaging approach (2500 fps in our example).

  7. Acquisition method improvement for Bossa Nova Technologies' full Stokes, passive polarization imaging camera SALSA

    NASA Astrophysics Data System (ADS)

    El Ketara, M.; Vedel, M.; Breugnot, S.

    2016-05-01

    For some applications, the need for fast polarization acquisition is essential (if the scene observed is moving or changing quickly). In this paper, we present a new acquisition method for Bossa Nova Technologies' full Stokes passive polarization imaging camera, the SALSA. This polarization imaging camera is based on "Division of Time polarimetry" architecture. The use of this technique presents the advantage of preserving the full resolution of the image observed all the while reducing the speed acquisition time. The goal of this new acquisition method is to overcome the limitations associated with Division of Time acquisition technique as well as to obtain high-speed polarization imaging while maintaining the image resolution. The efficiency of this new method is demonstrated in this paper through different experiments.

  8. Biometric iris image acquisition system with wavefront coding technology

    NASA Astrophysics Data System (ADS)

    Hsieh, Sheng-Hsun; Yang, Hsi-Wen; Huang, Shao-Hung; Li, Yung-Hui; Tien, Chung-Hao

    2013-09-01

    Biometric signatures for identity recognition have been practiced for centuries. Basically, the personal attributes used for a biometric identification system can be classified into two areas: one is based on physiological attributes, such as DNA, facial features, retinal vasculature, fingerprint, hand geometry, iris texture and so on; the other scenario is dependent on the individual behavioral attributes, such as signature, keystroke, voice and gait style. Among these features, iris recognition is one of the most attractive approaches due to its nature of randomness, texture stability over a life time, high entropy density and non-invasive acquisition. While the performance of iris recognition on high quality image is well investigated, not too many studies addressed that how iris recognition performs subject to non-ideal image data, especially when the data is acquired in challenging conditions, such as long working distance, dynamical movement of subjects, uncontrolled illumination conditions and so on. There are three main contributions in this paper. Firstly, the optical system parameters, such as magnification and field of view, was optimally designed through the first-order optics. Secondly, the irradiance constraints was derived by optical conservation theorem. Through the relationship between the subject and the detector, we could estimate the limitation of working distance when the camera lens and CCD sensor were known. The working distance is set to 3m in our system with pupil diameter 86mm and CCD irradiance 0.3mW/cm2. Finally, We employed a hybrid scheme combining eye tracking with pan and tilt system, wavefront coding technology, filter optimization and post signal recognition to implement a robust iris recognition system in dynamic operation. The blurred image was restored to ensure recognition accuracy over 3m working distance with 400mm focal length and aperture F/6.3 optics. The simulation result as well as experiment validates the proposed code

  9. Star sensor image acquisition and preprocessing hardware system based on CMOS image sensor and FGPA

    NASA Astrophysics Data System (ADS)

    Hao, Xuetao; Jiang, Jie; Zhang, Guangjun

    2003-09-01

    Star Sensor is an avionics instrument used to provide the absolute 3-axis attitude of a spacecraft utilizing star observations. It consists of an electronic camera and associated processing electronics. As outcome of advancing state-of-the-art, new generation star sensor features faster, lower cost, power dissipation and size than the first generation star sensor. This paper describes a star sensor anterior image acquisition and pre-processing hardware system based on CMOS image-sensor and FPGA technology. Practically, star images are produced by a simple simulator on PC, acquired by CMOS image sensor, pre-processed by FPGA, saved in SRAM, read out by EPP protocol and validated by an image process software on PC. The hardware part of system acquires images thought CMOS image-sensor controlled by FPGA, then processes image data by a circuit module of FPGA, and save images to SRAM for test. Basic image data for star recognition and attitude determination of spacecrafts are provided by it. As an important reference for developing star sensor prototype, the system validates the performance advantages of new generation star sensor.

  10. High-speed image acquisition technology in quality detection of workpiece surface

    NASA Astrophysics Data System (ADS)

    Wu, Kaihua; Jin, Zexuan; Wang, Wenjie; Chen, Nian

    2016-11-01

    High-speed image acquisition technology has a great significance to improve the effciency of the workpiece surface quality detection, image quality directly affects the final test results. Aiming at the high-speed image acquisition of workpiece surface quality online detection, a workpiece image high-speed online acquisition method was produced. A high-speed online image acquisition sequence was designed. The quantitative relationship between the positioning accuracy in the high speed online image acquisition, motion blur, exposure time and the speed of workpiece was analyzed. The effect between the vibration between transfer mechanism and workpiece was analyzed. Fast trigger was implemented by photoelectric sensor. The accurate positioning was implemented by using the high accuracy time delay module. The motion blur was controlled by reducing the exposure time. A high-speed image acquisition system was designed based on the high-speed image acquisition method. The positioning accuracy was less than 0.1 mm, and the motion blur was less than one pixel.

  11. Web-based data acquisition and management system for GOSAT validation Lidar data analysis

    NASA Astrophysics Data System (ADS)

    Okumura, Hiroshi; Takubo, Shoichiro; Kawasaki, Takeru; Abdullah, Indra N.; Uchino, Osamu; Morino, Isamu; Yokota, Tatsuya; Nagai, Tomohiro; Sakai, Tetsu; Maki, Takashi; Arai, Kohei

    2012-11-01

    An web-base data acquisition and management system for GOSAT (Greenhouse gases Observation SATellite) validation lidar data analysis is developed. The system consists of data acquisition sub-system (DAS) and data management sub-system (DMS). DAS written in Perl language acquires AMeDAS ground-level meteorological data, Rawinsonde upper-air meteorological data, ground-level oxidant data, skyradiometer data, skyview camera images, meteorological satellite IR image data and GOSAT validation lidar data. DMS written in PHP language demonstrates satellite-pass date and all acquired data.

  12. Image-analysis library

    NASA Technical Reports Server (NTRS)

    1980-01-01

    MATHPAC image-analysis library is collection of general-purpose mathematical and statistical routines and special-purpose data-analysis and pattern-recognition routines for image analysis. MATHPAC library consists of Linear Algebra, Optimization, Statistical-Summary, Densities and Distribution, Regression, and Statistical-Test packages.

  13. Analysis of an antijam FH acquisition scheme

    NASA Astrophysics Data System (ADS)

    Miller, Leonard E.; Lee, Jhong S.; French, Robert H.; Torrieri, Don J.

    1992-01-01

    An easily implemented matched filter scheme for acquiring hopping code synchronization of incoming frequency-hopping (FH) signals is analyzed, and its performance is evaluated for two types of jamming: partial-band noise jamming and partial-band multitone jamming. The system is designed to reduce jammer-induced false alarms. The system's matched-filter output is compared to an adaptive threshold that is derived from a measurement of the number of acquisition channels being jammed. Example performance calculations are given for the frequency coverage of the jamming either fixed over the entire acquisition period or hopped, that is, changed for each acquisition pulse. It is shown that the jammer's optimum strategy (the worst case) is to maximize the false-alarm probability without regard for the effect on detection probability, for both partial-band noise and multi-tone jamming. It is also shown that a significantly lower probability of false acquisition results from using an adaptive matched-filter threshold, demonstrating that the strategy studied here is superior to conventional nonadaptive threshold schemes.

  14. Basics of image analysis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral imaging technology has emerged as a powerful tool for quality and safety inspection of food and agricultural products and in precision agriculture over the past decade. Image analysis is a critical step in implementing hyperspectral imaging technology; it is aimed to improve the qualit...

  15. Dual-energy imaging of the chest: Optimization of image acquisition techniques for the 'bone-only' image

    SciTech Connect

    Shkumat, N. A.; Siewerdsen, J. H.; Richard, S.; Paul, N. S.; Yorkston, J.; Van Metter, R.

    2008-02-15

    Experiments were conducted to determine optimal acquisition techniques for bone image decompositions for a prototype dual-energy (DE) imaging system. Technique parameters included kVp pair (denoted [kVp{sup L}/kVp{sup H}]) and dose allocation (the proportion of dose in low- and high-energy projections), each optimized to provide maximum signal difference-to-noise ratio in DE images. Experiments involved a chest phantom representing an average patient size and containing simulated ribs and lung nodules. Low- and high-energy kVp were varied from 60-90 and 120-150 kVp, respectively. The optimal kVp pair was determined to be [60/130] kVp, with image quality showing a strong dependence on low-kVp selection. Optimal dose allocation was approximately 0.5--i.e., an equal dose imparted by the low- and high-energy projections. The results complement earlier studies of optimal DE soft-tissue image acquisition, with differences attributed to the specific imaging task. Together, the results help to guide the development and implementation of high-performance DE imaging systems, with applications including lung nodule detection and diagnosis, pneumothorax identification, and musculoskeletal imaging (e.g., discrimination of rib fractures from metastasis)

  16. Dual-energy imaging of the chest: optimization of image acquisition techniques for the 'bone-only' image.

    PubMed

    Shkumat, N A; Siewerdsen, J H; Richard, S; Paul, N S; Yorkston, J; Van Metter, R

    2008-02-01

    Experiments were conducted to determine optimal acquisition techniques for bone image decompositions for a prototype dual-energy (DE) imaging system. Technique parameters included kVp pair (denoted [kVp(L)/kVp(H)]) and dose allocation (the proportion of dose in low- and high-energy projections), each optimized to provide maximum signal difference-to-noise ratio in DE images. Experiments involved a chest phantom representing an average patient size and containing simulated ribs and lung nodules. Low- and high-energy kVp were varied from 60-90 and 120-150 kVp, respectively. The optimal kVp pair was determined to be [60/130] kVp, with image quality showing a strong dependence on low-kVp selection. Optimal dose allocation was approximately 0.5-i.e., an equal dose imparted by the low- and high-energy projections. The results complement earlier studies of optimal DE soft-tissue image acquisition, with differences attributed to the specific imaging task. Together, the results help to guide the development and implementation of high-performance DE imaging systems, with applications including lung nodule detection and diagnosis, pneumothorax identification, and musculoskeletal imaging (e.g., discrimination of rib fractures from metastasis).

  17. Coherent diffraction microscopy at SPring-8: instrumentation, data acquisition and data analysis.

    PubMed

    Xu, Rui; Salha, Sara; Raines, Kevin S; Jiang, Huaidong; Chen, Chien Chun; Takahashi, Yukio; Kohmura, Yoshiki; Nishino, Yoshinori; Song, Changyong; Ishikawa, Tetsuya; Miao, Jianwei

    2011-03-01

    Since the first demonstration of coherent diffraction microscopy in 1999, this lensless imaging technique has been experimentally refined by continued developments. Here, instrumentation and experimental procedures for measuring oversampled diffraction patterns from non-crystalline specimens using an undulator beamline (BL29XUL) at SPring-8 are presented. In addition, detailed post-experimental data analysis is provided that yields high-quality image reconstructions. As the acquisition of high-quality diffraction patterns is at least as important as the phase-retrieval procedure to guarantee successful image reconstructions, this work will be of interest for those who want to apply this imaging technique to materials science and biological samples.

  18. Dynamic whole-body PET parametric imaging: I. Concept, acquisition protocol optimization and clinical application

    NASA Astrophysics Data System (ADS)

    Karakatsanis, Nicolas A.; Lodge, Martin A.; Tahari, Abdel K.; Zhou, Y.; Wahl, Richard L.; Rahmim, Arman

    2013-10-01

    Static whole-body PET/CT, employing the standardized uptake value (SUV), is considered the standard clinical approach to diagnosis and treatment response monitoring for a wide range of oncologic malignancies. Alternative PET protocols involving dynamic acquisition of temporal images have been implemented in the research setting, allowing quantification of tracer dynamics, an important capability for tumor characterization and treatment response monitoring. Nonetheless, dynamic protocols have been confined to single-bed-coverage limiting the axial field-of-view to ˜15-20 cm, and have not been translated to the routine clinical context of whole-body PET imaging for the inspection of disseminated disease. Here, we pursue a transition to dynamic whole-body PET parametric imaging, by presenting, within a unified framework, clinically feasible multi-bed dynamic PET acquisition protocols and parametric imaging methods. We investigate solutions to address the challenges of: (i) long acquisitions, (ii) small number of dynamic frames per bed, and (iii) non-invasive quantification of kinetics in the plasma. In the present study, a novel dynamic (4D) whole-body PET acquisition protocol of ˜45 min total length is presented, composed of (i) an initial 6 min dynamic PET scan (24 frames) over the heart, followed by (ii) a sequence of multi-pass multi-bed PET scans (six passes × seven bed positions, each scanned for 45 s). Standard Patlak linear graphical analysis modeling was employed, coupled with image-derived plasma input function measurements. Ordinary least squares Patlak estimation was used as the baseline regression method to quantify the physiological parameters of tracer uptake rate Ki and total blood distribution volume V on an individual voxel basis. Extensive Monte Carlo simulation studies, using a wide set of published kinetic FDG parameters and GATE and XCAT platforms, were conducted to optimize the acquisition protocol from a range of ten different clinically

  19. NOVA-NREL Optimal Vehicle Acquisition Analysis (Brochure)

    SciTech Connect

    Blakley, H.

    2011-03-01

    Federal fleet managers face unique challenges in accomplishing their mission - meeting agency transportation needs while complying with Federal goals and mandates. Included in these challenges are a variety of statutory requirements, executive orders, and internal goals and objectives that typically focus on petroleum consumption and greenhouse gas (GHG) emissions reductions, alternative fuel vehicle (AFV) acquisitions, and alternative fuel use increases. Given the large number of mandates affecting Federal fleets and the challenges faced by all fleet managers in executing day-to-day operations, a primary challenge for agencies and other organizations is ensuring that they are as efficient as possible in using constrained fleet budgets. An NREL Optimal Vehicle Acquisition (NOVA) analysis makes use of a mathematical model with a variety of fleet-related data to create an optimal vehicle acquisition strategy for a given goal, such as petroleum or GHG reduction. The analysis can helps fleets develop a vehicle acquisition strategy that maximizes petroleum and greenhouse gas reductions.

  20. Research on remote sensing image pixel attribute data acquisition method in AutoCAD

    NASA Astrophysics Data System (ADS)

    Liu, Xiaoyang; Sun, Guangtong; Liu, Jun; Liu, Hui

    2013-07-01

    The remote sensing image has been widely used in AutoCAD, but AutoCAD lack of the function of remote sensing image processing. In the paper, ObjectARX was used for the secondary development tool, combined with the Image Engine SDK to realize remote sensing image pixel attribute data acquisition in AutoCAD, which provides critical technical support for AutoCAD environment remote sensing image processing algorithms.

  1. Multispectral integral imaging acquisition and processing using a monochrome camera and a liquid crystal tunable filter.

    PubMed

    Latorre-Carmona, Pedro; Sánchez-Ortiga, Emilio; Xiao, Xiao; Pla, Filiberto; Martínez-Corral, Manuel; Navarro, Héctor; Saavedra, Genaro; Javidi, Bahram

    2012-11-05

    This paper presents an acquisition system and a procedure to capture 3D scenes in different spectral bands. The acquisition system is formed by a monochrome camera, and a Liquid Crystal Tunable Filter (LCTF) that allows to acquire images at different spectral bands in the [480, 680]nm wavelength interval. The Synthetic Aperture Integral Imaging acquisition technique is used to obtain the elemental images for each wavelength. These elemental images are used to computationally obtain the reconstruction planes of the 3D scene at different depth planes. The 3D profile of the acquired scene is also obtained using a minimization of the variance of the contribution of the elemental images at each image pixel. Experimental results show the viability to recover the 3D multispectral information of the scene. Integration of 3D and multispectral information could have important benefits in different areas, including skin cancer detection, remote sensing and pattern recognition, among others.

  2. Multisensor Image Analysis System

    DTIC Science & Technology

    1993-04-15

    AD-A263 679 II Uli! 91 Multisensor Image Analysis System Final Report Authors. Dr. G. M. Flachs Dr. Michael Giles Dr. Jay Jordan Dr. Eric...or decision, unless so designated by other documentation. 93-09739 *>ft s n~. now illlllM3lMVf Multisensor Image Analysis System Final...Multisensor Image Analysis System 3. REPORT TYPE AND DATES COVERED FINAL: LQj&tt-Z JZOfVL 5. FUNDING NUMBERS 93 > 6. AUTHOR(S) Drs. Gerald

  3. High resolution x-ray medical sequential image acquisition and processing system based on PCI interface

    NASA Astrophysics Data System (ADS)

    Lu, Dongming; Chen, Qian; Gu, Guohua

    2003-11-01

    In the field of medical application, it is of great importance to adopt digital image processing technique. Based on the characteristics of medical image, we introduced the digital image processing method to the X-ray imaging system, and developed a high resolution x-ray medical sequential image acquisition and processing system that employs image enhancer and CCD. This system consists of three basic modules, namely sequential image acquisition, data transfer and system control, and image processing. Under the control of FPGA (Field Programmable Gate Array), images acquired by the front-end circuit are transmitted to a PC through high speed PCI bus, and then optimized by the image processing program. The software kits, which include PCI Device Driver and Image Processing Package, are developed with Visual C++ Language based on Windows OS. In this paper, we present a general introduction to the principle and the operating procedure of X-ray Sequential Image Acquisition and Processing System, with special emphasis on the key issues of the hardware design. In addition, the context, principle, status quo and the digitizing trend of X-ray Imaging are explained succinctly. Finally, the preliminary experimental results are shown to demonstrate that the system is capable of achieving high quality X-ray sequential images.

  4. Development of data acquisition and analysis software for multichannel detectors

    SciTech Connect

    Chung, Y.

    1988-06-01

    This report describes the development of data acquisition and analysis software for Apple Macintosh computers, capable of controlling two multichannel detectors. With the help of outstanding graphics capabilities, easy-to-use user interface, and several other built-in convenience features, this application has enhanced the productivity and the efficiency of data analysis. 2 refs., 6 figs.

  5. 77 FR 40552 - Federal Acquisition Regulation; Price Analysis Techniques

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-10

    ...)(1), which sets forth the requirements of adequate price competition. However, only FAR 15.403-1(c)(1... Federal Acquisition Regulation; Price Analysis Techniques AGENCY: Department of Defense (DoD), General... clarify the use of a price analysis technique in order to establish a fair and reasonable price....

  6. Progress in visualizing turbulent flow using single-echo acquisition imaging.

    PubMed

    Wright, Steven M; McDougall, Mary Preston; Bosshard, John C

    2006-01-01

    MRI of flow remains a challenging problem despite significant improvements in imaging speeds. For periodic flow the acquisition can be gated, synchronizing data acquisition with the flow. However, this method fails to work if the flow is sufficiently fast that turbulence occurs, or when it is sufficiently fast that blurring occurs during the excitation of the spins or the acquisition of the signal. This paper describes recent progress in employing a very fast MR imaging technique, Single Echo Acquisition Imaging (SEA-MRI) and spin-tagging to visualize very rapid and turbulent flow patterns. Demonstrations are done on a separating channel phantom with input flow rates ranging from zero to over 100 cm/sec. Spin-tagging enables a "texture" to be placed on the spins, enabling clear visualization of the complex flow patterns, and in some cases measurement of the flow velocity.

  7. Data acquisition and processing system of the electron cyclotron emission imaging system of the KSTAR tokamak.

    PubMed

    Kim, J B; Lee, W; Yun, G S; Park, H K; Domier, C W; Luhmann, N C

    2010-10-01

    A new innovative electron cyclotron emission imaging (ECEI) diagnostic system for the Korean Superconducting Tokamak Advanced Research (KSTAR) produces a large amount of data. The design of the data acquisition and processing system of the ECEI diagnostic system should consider covering the large data production and flow. The system design is based on the layered structure scalable to the future extension to accommodate increasing data demands. Software architecture that allows a web-based monitoring of the operation status, remote experiment, and data analysis is discussed. The operating software will help machine operators and users validate the acquired data promptly, prepare next discharge, and enhance the experiment performance and data analysis in a distributed environment.

  8. Impact of image acquisition on voxel-based-morphometry investigations of age-related structural brain changes.

    PubMed

    Streitbürger, Daniel-Paolo; Pampel, André; Krueger, Gunnar; Lepsien, Jöran; Schroeter, Matthias L; Mueller, Karsten; Möller, Harald E

    2014-02-15

    A growing number of magnetic resonance imaging studies employ voxel-based morphometry (VBM) to assess structural brain changes. Recent reports have shown that image acquisition parameters may influence VBM results. For systematic evaluation, gray-matter-density (GMD) changes associated with aging were investigated by VBM employing acquisitions with different radiofrequency head coils (12-channel matrix coil vs. 32-channel array), different pulse sequences (MP-RAGE vs. MP2RAGE), and different voxel dimensions (1mm vs. 0.8mm). Thirty-six healthy subjects, classified as young, middle-aged, or elderly, participated in the study. Two-sample and paired t-tests revealed significant effects of acquisition parameters (coil, pulse sequence, and resolution) on the estimated age-related GMD changes in cortical and subcortical regions. Potential advantages in tissue classification and segmentation were obtained for MP2RAGE. The 32-channel coil generally outperformed the 12-channel coil, with more benefit for MP2RAGE. Further improvement can be expected from higher resolution if the loss in SNR is accounted for. Use of inconsistent acquisition parameters in VBM analyses is likely to introduce systematic bias. Overall, acquisition and protocol changes require careful adaptations of the VBM analysis strategy before generalized conclusion can be drawn.

  9. Reference radiochromic film dosimetry in kilovoltage photon beams during CBCT image acquisition

    SciTech Connect

    Tomic, Nada; Devic, Slobodan; DeBlois, Francois; Seuntjens, Jan

    2010-03-15

    Purpose: A common approach for dose assessment during cone beam computed tomography (CBCT) acquisition is to use thermoluminescent detectors for skin dose measurements (on patients or phantoms) or ionization chamber (in phantoms) for body dose measurements. However, the benefits of a daily CBCT image acquisition such as margin reduction in planning target volume and the image quality must be weighted against the extra dose received during CBCT acquisitions. Methods: The authors describe a two-dimensional reference dosimetry technique for measuring dose from CBCT scans using the on-board imaging system on a Varian Clinac-iX linear accelerator that employs the XR-QA radiochromic film model, specifically designed for dose measurements at low energy photons. The CBCT dose measurements were performed for three different body regions (head and neck, pelvis, and thorax) using humanoid Rando phantom. Results: The authors report on both surface dose and dose profiles measurements during clinical CBCT procedures carried out on a humanoid Rando phantom. Our measurements show that the surface doses per CBCT scan can range anywhere between 0.1 and 4.7 cGy, with the lowest surface dose observed in the head and neck region, while the highest surface dose was observed for the Pelvis spot light CBCT protocol in the pelvic region, on the posterior side of the Rando phantom. The authors also present results of the uncertainty analysis of our XR-QA radiochromic film dosimetry system. Conclusions: Radiochromic film dosimetry protocol described in this work was used to perform dose measurements during CBCT acquisitions with the one-sigma dose measurement uncertainty of up to 3% for doses above 1 cGy. Our protocol is based on film exposure calibration in terms of ''air kerma in air,'' which simplifies both the calibration procedure and reference dosimetry measurements. The results from a full Monte Carlo investigation of the dose conversion of measured XR-QA film dose at the surface into

  10. An evaluation on CT image acquisition method for medical VR applications

    NASA Astrophysics Data System (ADS)

    Jang, Seong-wook; Ko, Junho; Yoo, Yon-sik; Kim, Yoonsang

    2017-02-01

    Recent medical virtual reality (VR) applications to minimize re-operations are being studied for improvements in surgical efficiency and reduction of operation error. The CT image acquisition method considering three-dimensional (3D) modeling for medical VR applications is important, because the realistic model is required for the actual human organ. However, the research for medical VR applications has focused on 3D modeling techniques and utilized 3D models. In addition, research on a CT image acquisition method considering 3D modeling has never been reported. The conventional CT image acquisition method involves scanning a limited area of the lesion for the diagnosis of doctors once or twice. However, the medical VR application is required to acquire the CT image considering patients' various postures and a wider area than the lesion. A wider area than the lesion is required because of the necessary process of comparing bilateral sides for dyskinesia diagnosis of the shoulder, pelvis, and leg. Moreover, patients' various postures are required due to the different effects on the musculoskeletal system. Therefore, in this paper, we perform a comparative experiment on the acquired CT images considering image area (unilateral/bilateral) and patients' postures (neutral/abducted). CT images are acquired from 10 patients for the experiments, and the acquired CT images are evaluated based on the length per pixel and the morphological deviation. Finally, by comparing the experiment results, we evaluate the CT image acquisition method for medical VR applications.

  11. Digital-image processing and image analysis of glacier ice

    USGS Publications Warehouse

    Fitzpatrick, Joan J.

    2013-01-01

    This document provides a methodology for extracting grain statistics from 8-bit color and grayscale images of thin sections of glacier ice—a subset of physical properties measurements typically performed on ice cores. This type of analysis is most commonly used to characterize the evolution of ice-crystal size, shape, and intercrystalline spatial relations within a large body of ice sampled by deep ice-coring projects from which paleoclimate records will be developed. However, such information is equally useful for investigating the stress state and physical responses of ice to stresses within a glacier. The methods of analysis presented here go hand-in-hand with the analysis of ice fabrics (aggregate crystal orientations) and, when combined with fabric analysis, provide a powerful method for investigating the dynamic recrystallization and deformation behaviors of bodies of ice in motion. The procedures described in this document compose a step-by-step handbook for a specific image acquisition and data reduction system built in support of U.S. Geological Survey ice analysis projects, but the general methodology can be used with any combination of image processing and analysis software. The specific approaches in this document use the FoveaPro 4 plug-in toolset to Adobe Photoshop CS5 Extended but it can be carried out equally well, though somewhat less conveniently, with software such as the image processing toolbox in MATLAB, Image-Pro Plus, or ImageJ.

  12. Imageability predicts the age of acquisition of verbs in Chinese children.

    PubMed

    Ma, Weiyi; Golinkoff, Roberta Michnick; Hirsh-Pasek, Kathy; McDonough, Colleen; Tardif, Twila

    2009-03-01

    Verbs are harder to learn than nouns in English and in many other languages, but are relatively easy to learn in Chinese. This paper evaluates one potential explanation for these findings by examining the construct of imageability, or the ability of a word to produce a mental image. Chinese adults rated the imageability of Chinese words from the Chinese Communicative Development Inventory (Tardif et al., in press). Imageability ratings were a reliable predictor of age of acquisition in Chinese for both nouns and verbs. Furthermore, whereas early Chinese and English nouns do not differ in imageability, verbs receive higher imageability ratings in Chinese than in English. Compared with input frequency, imageability independently accounts for a portion of the variance in age of acquisition (AoA) of verb learning in Chinese and English.

  13. Design of a Remote Infrared Images and Other Data Acquisition Station for outdoor applications

    NASA Astrophysics Data System (ADS)

    Béland, M.-A.; Djupkep, F. B. D.; Bendada, A.; Maldague, X.; Ferrarini, G.; Bison, P.; Grinzato, E.

    2013-05-01

    The Infrared Images and Other Data Acquisition Station enables a user, who is located inside a laboratory, to acquire visible and infrared images and distances in an outdoor environment with the help of an Internet connection. This station can acquire data using an infrared camera, a visible camera, and a rangefinder. The system can be used through a web page or through Python functions.

  14. Data Acquisition and Image Reconstruction Systems from the miniPET Scanners to the CARDIOTOM Camera

    NASA Astrophysics Data System (ADS)

    Valastván, I.; Imrek, J.; Hegyesi, G.; Molnár, J.; Novák, D.; Bone, D.; Kerek, A.

    2007-11-01

    Nuclear imaging devices play an important role in medical diagnosis as well as drug research. The first and second generation data acquisition systems and the image reconstruction library developed provide a unified hardware and software platform for the miniPET-I, miniPET-II small animal PET scanners and for the CARDIOTOM™.

  15. An Analysis of Test And Evaluation in Rapid Acquisition Programs

    DTIC Science & Technology

    2015-12-01

    United States. Chemical testing took place at Dugway Proving Grounds and the Edgewood Chemical Biological Center, trace explosive testing took place...system in controlled conditions where measurable data was collected. Several instruments were tested at Dugway Proving Grounds using Chemical ...NAVAL POSTGRADUATE SCHOOL MONTEREY, CALIFORNIA JOINT APPLIED PROJECT AN ANALYSIS OF TEST AND EVALUATION IN RAPID ACQUISITION

  16. ACQ4: an open-source software platform for data acquisition and analysis in neurophysiology research

    PubMed Central

    Campagnola, Luke; Kratz, Megan B.; Manis, Paul B.

    2014-01-01

    The complexity of modern neurophysiology experiments requires specialized software to coordinate multiple acquisition devices and analyze the collected data. We have developed ACQ4, an open-source software platform for performing data acquisition and analysis in experimental neurophysiology. This software integrates the tasks of acquiring, managing, and analyzing experimental data. ACQ4 has been used primarily for standard patch-clamp electrophysiology, laser scanning photostimulation, multiphoton microscopy, intrinsic imaging, and calcium imaging. The system is highly modular, which facilitates the addition of new devices and functionality. The modules included with ACQ4 provide for rapid construction of acquisition protocols, live video display, and customizable analysis tools. Position-aware data collection allows automated construction of image mosaics and registration of images with 3-dimensional anatomical atlases. ACQ4 uses free and open-source tools including Python, NumPy/SciPy for numerical computation, PyQt for the user interface, and PyQtGraph for scientific graphics. Supported hardware includes cameras, patch clamp amplifiers, scanning mirrors, lasers, shutters, Pockels cells, motorized stages, and more. ACQ4 is available for download at http://www.acq4.org. PMID:24523692

  17. ACQ4: an open-source software platform for data acquisition and analysis in neurophysiology research.

    PubMed

    Campagnola, Luke; Kratz, Megan B; Manis, Paul B

    2014-01-01

    The complexity of modern neurophysiology experiments requires specialized software to coordinate multiple acquisition devices and analyze the collected data. We have developed ACQ4, an open-source software platform for performing data acquisition and analysis in experimental neurophysiology. This software integrates the tasks of acquiring, managing, and analyzing experimental data. ACQ4 has been used primarily for standard patch-clamp electrophysiology, laser scanning photostimulation, multiphoton microscopy, intrinsic imaging, and calcium imaging. The system is highly modular, which facilitates the addition of new devices and functionality. The modules included with ACQ4 provide for rapid construction of acquisition protocols, live video display, and customizable analysis tools. Position-aware data collection allows automated construction of image mosaics and registration of images with 3-dimensional anatomical atlases. ACQ4 uses free and open-source tools including Python, NumPy/SciPy for numerical computation, PyQt for the user interface, and PyQtGraph for scientific graphics. Supported hardware includes cameras, patch clamp amplifiers, scanning mirrors, lasers, shutters, Pockels cells, motorized stages, and more. ACQ4 is available for download at http://www.acq4.org.

  18. Design of area array CCD image acquisition and display system based on FPGA

    NASA Astrophysics Data System (ADS)

    Li, Lei; Zhang, Ning; Li, Tianting; Pan, Yue; Dai, Yuming

    2014-09-01

    With the development of science and technology, CCD(Charge-coupled Device) has been widely applied in various fields and plays an important role in the modern sensing system, therefore researching a real-time image acquisition and display plan based on CCD device has great significance. This paper introduces an image data acquisition and display system of area array CCD based on FPGA. Several key technical challenges and problems of the system have also been analyzed and followed solutions put forward .The FPGA works as the core processing unit in the system that controls the integral time sequence .The ICX285AL area array CCD image sensor produced by SONY Corporation has been used in the system. The FPGA works to complete the driver of the area array CCD, then analog front end (AFE) processes the signal of the CCD image, including amplification, filtering, noise elimination, CDS correlation double sampling, etc. AD9945 produced by ADI Corporation to convert analog signal to digital signal. Developed Camera Link high-speed data transmission circuit, and completed the PC-end software design of the image acquisition, and realized the real-time display of images. The result through practical testing indicates that the system in the image acquisition and control is stable and reliable, and the indicators meet the actual project requirements.

  19. Content Analysis in Systems Engineering Acquisition Activities

    DTIC Science & Technology

    2016-04-30

    shape requirements definitions for system upgrade or modification contracts and new baseline contracts. Finally, content analysis training and skill...back to the system designers, this information can then be used to shape requirements definition for system upgrade or modification contracts and new...Activity System Requirements Definition Ensuring the system requirements adequately reflect the stakeholder requirements Negotiating modifications to

  20. Image and Sensor Data Processing for Target Acquisition and Recognition.

    DTIC Science & Technology

    1980-11-01

    traitements sont effectuds sur des images fournies par des capteurs fonctionrnant A des longueurs d’onde voisines de 4 u et 10 p Ces images diff~rent des...bruit, doe aux limites physiques et technologiques des capteurs - d’autre part, une qualitd essentielle pour les applications militaires. qui provient de...diminuer Ileffet de certains ddfauts qui affectent l’image originale et qui sont dus aux imperfections du capteur (chapitre 1) - La d~tection des cibles

  1. Rapid acquisition of in vivo biological images by use of optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Tearney, G. J.; Bouma, B. E.; Boppart, S. A.; Golubovic, B.; Swanson, E. A.; Fujimoto, J. G.

    1996-09-01

    The development of techniques for high-speed image acquisition in optical coherence tomography (OCT) systems is essential for suppressing motion artifacts when one is imaging living systems. We describe a new OCT system for performing micrometer-scale, cross-sectional optical imaging at four images / s. To achieve OCT image-acquisition times of less than 1 s, we use a piezoelectric fiber stretcher to vary the reference arm delay. A Kerr-lens mode-locked chromium-doped forsterite laser is employed as the low-coherence source for the high-speed OCT system. Dynamic, motion-artifact-free in vivo imaging of a beating Xenopus laevis (African frog) heart is demonstrated.

  2. Temporally-structured acquisition of multidimensional optical imaging data facilitates visualization of elusive cortical representations in the behaving monkey.

    PubMed

    Omer, David B; Hildesheim, Rina; Grinvald, Amiram

    2013-11-15

    Fundamental understanding of higher cognitive functions can greatly benefit from imaging of cortical activity with high spatiotemporal resolution in the behaving non-human primate. To achieve rapid imaging of high-resolution dynamics of cortical representations of spontaneous and evoked activity, we designed a novel data acquisition protocol for sensory stimulation by rapidly interleaving multiple stimuli in continuous sessions of optical imaging with voltage-sensitive dyes. We also tested a new algorithm for the "temporally structured component analysis" (TSCA) of a multidimensional time series that was developed for our new data acquisition protocol, but was tested only on simulated data (Blumenfeld, 2010). In addition to the raw data, the algorithm incorporates prior knowledge about the temporal structure of the data as well as input from other information. Here we showed that TSCA can successfully separate functional signal components from other signals referred to as noise. Imaging of responses to multiple visual stimuli, utilizing voltage-sensitive dyes, was performed on the visual cortex of awake monkeys. Multiple cortical representations, including orientation and ocular dominance maps as well as the hitherto elusive retinotopic representation of orientation stimuli, were extracted in only 10s of imaging, approximately two orders of magnitude faster than accomplished by conventional methods. Since the approach is rather general, other imaging techniques may also benefit from the same stimulation protocol. This methodology can thus facilitate rapid optical imaging explorations in monkeys, rodents and other species with a versatility and speed that were not feasible before.

  3. An integrated data acquisition and analysis system at GSI

    SciTech Connect

    Essel, H.G.; Hoffmann, J.; Richter, M.; Sohlbach, H.; Spreng )

    1989-10-01

    GOOSY, the GSI data acquisition and analysis system has been extended to handle experimental set-ups and data from heavy-ion experiments at high energies. A new front-end system has been designed and built. It is a VME based multi-processor system connected to CAMAC and FASTBUS via standard VSB. This system allows pre-analysis and filtering of the raw data. The architecture of the system is presented and a status report is given in this paper.

  4. FABIA: factor analysis for bicluster acquisition

    PubMed Central

    Hochreiter, Sepp; Bodenhofer, Ulrich; Heusel, Martin; Mayr, Andreas; Mitterecker, Andreas; Kasim, Adetayo; Khamiakova, Tatsiana; Van Sanden, Suzy; Lin, Dan; Talloen, Willem; Bijnens, Luc; Göhlmann, Hinrich W. H.; Shkedy, Ziv; Clevert, Djork-Arné

    2010-01-01

    Motivation: Biclustering of transcriptomic data groups genes and samples simultaneously. It is emerging as a standard tool for extracting knowledge from gene expression measurements. We propose a novel generative approach for biclustering called ‘FABIA: Factor Analysis for Bicluster Acquisition’. FABIA is based on a multiplicative model, which accounts for linear dependencies between gene expression and conditions, and also captures heavy-tailed distributions as observed in real-world transcriptomic data. The generative framework allows to utilize well-founded model selection methods and to apply Bayesian techniques. Results: On 100 simulated datasets with known true, artificially implanted biclusters, FABIA clearly outperformed all 11 competitors. On these datasets, FABIA was able to separate spurious biclusters from true biclusters by ranking biclusters according to their information content. FABIA was tested on three microarray datasets with known subclusters, where it was two times the best and once the second best method among the compared biclustering approaches. Availability: FABIA is available as an R package on Bioconductor (http://www.bioconductor.org). All datasets, results and software are available at http://www.bioinf.jku.at/software/fabia/fabia.html Contact: hochreit@bioinf.jku.at Supplementary information: Supplementary data are available at Bioinformatics online. PMID:20418340

  5. Complex-valued acquisition of the diffraction imaging by incoherent quasimonochromatic light without a support constraint

    SciTech Connect

    Zhang Minghui; Xu Jianfei; Wang Xianfu; Wei Qing

    2010-10-15

    A scheme for complex-valued acquisition of the diffraction imaging with quasimonochromatic incoherent light is theoretically proposed. The main idea is to project the real and the imaginary parts of a Fraunhofer diffraction field on intensity distributions, respectively, with the use of a {pi}/2 phase-changing plate. The whole procedure is iterative algorithm free and needs no a priori knowledge of an arbitrary object. A numerical experiment and a quantitative confirmation are also given. To our knowledge, it was the first physical proposal for the complex-valued acquisition of a diffraction imaging by two-dimensional coherent patterns with thermal illumination.

  6. Research of aerial imaging spectrometer data acquisition technology based on USB 3.0

    NASA Astrophysics Data System (ADS)

    Huang, Junze; Wang, Yueming; He, Daogang; Yu, Yanan

    2016-11-01

    With the emergence of UAV (unmanned aerial vehicle) platform for aerial imaging spectrometer, research of aerial imaging spectrometer DAS(data acquisition system) faces new challenges. Due to the limitation of platform and other factors, the aerial imaging spectrometer DAS requires small-light, low-cost and universal. Traditional aerial imaging spectrometer DAS system is expensive, bulky, non-universal and unsupported plug-and-play based on PCIe. So that has been unable to meet promotion and application of the aerial imaging spectrometer. In order to solve these problems, the new data acquisition scheme bases on USB3.0 interface.USB3.0 can provide guarantee of small-light, low-cost and universal relying on the forward-looking technology advantage. USB3.0 transmission theory is up to 5Gbps.And the GPIF programming interface achieves 3.2Gbps of the effective theoretical data bandwidth.USB3.0 can fully meet the needs of the aerial imaging spectrometer data transmission rate. The scheme uses the slave FIFO asynchronous data transmission mode between FPGA and USB3014 interface chip. Firstly system collects spectral data from TLK2711 of high-speed serial interface chip. Then FPGA receives data in DDR2 cache after ping-pong data processing. Finally USB3014 interface chip transmits data via automatic-dma approach and uploads to PC by USB3.0 cable. During the manufacture of aerial imaging spectrometer, the DAS can achieve image acquisition, transmission, storage and display. All functions can provide the necessary test detection for aerial imaging spectrometer. The test shows that system performs stable and no data lose. Average transmission speed and storage speed of writing SSD can stabilize at 1.28Gbps. Consequently ,this data acquisition system can meet application requirements for aerial imaging spectrometer.

  7. Detection of pseudoaneurysm of the left ventricle by fast imaging employing steady-state acquisition (FIESTA) magnetic resonance imaging.

    PubMed

    Rerkpattanapipat, Pairoj; Mazur, Wojciech; Link, Kerry M; Clark, Hollins P; Hundley, W Gregory

    2003-01-01

    This report highlights the importance of interpretating images throughout the course of a dobutamine MRI stress test. Upon review of the baseline images, the left ventricular (LV) endocardium was not well seen due to flow artifacts associated with low intracavitary blood-flow velocity resulting from a prior myocardial infarction. Physicians implemented a cine fast imaging employing steady-state acquisition (FIESTA) technique that was not subject to low flow artifact within the LV cavity. With heightened image clarity, physicians unexpectedly identified a LV pseudoaneurysm.

  8. Image analysis library software development

    NASA Technical Reports Server (NTRS)

    Guseman, L. F., Jr.; Bryant, J.

    1977-01-01

    The Image Analysis Library consists of a collection of general purpose mathematical/statistical routines and special purpose data analysis/pattern recognition routines basic to the development of image analysis techniques for support of current and future Earth Resources Programs. Work was done to provide a collection of computer routines and associated documentation which form a part of the Image Analysis Library.

  9. Development and application of a high speed digital data acquisition technique to study steam bubble collapse using particle image velocimetry

    SciTech Connect

    Schmidl, W.D.

    1992-08-01

    The use of a Particle Image Velocimetry (PIV) method, which uses digital cameras for data acquisition, for studying high speed fluid flows is usually limited by the digital camera's frame acquisition rate. The velocity of the fluid under study has to be limited to insure that the tracer seeds suspended in the fluid remain in the camera's focal plane for at least two consecutive images. However, the use of digital cameras for data acquisition is desirable to simplify and expedite the data analysis process. A technique was developed which will measure fluid velocities with PIV techniques using two successive digital images and two different framing rates simultaneously. The first part of the method will measure changes which occur to the flow field at the relatively slow framing rate of 53.8 ms. The second part will measure changes to the same flow field at the relatively fast framing rate of 100 to 320 [mu]s. The effectiveness of this technique was tested by studying the collapse of steam bubbles in a subcooled tank of water, a relatively high speed phenomena. The tracer particles were recorded and velocity vectors for the fluid were obtained far from the steam bubble collapse.

  10. Development and application of a high speed digital data acquisition technique to study steam bubble collapse using particle image velocimetry

    SciTech Connect

    Schmidl, W.D.

    1992-08-01

    The use of a Particle Image Velocimetry (PIV) method, which uses digital cameras for data acquisition, for studying high speed fluid flows is usually limited by the digital camera`s frame acquisition rate. The velocity of the fluid under study has to be limited to insure that the tracer seeds suspended in the fluid remain in the camera`s focal plane for at least two consecutive images. However, the use of digital cameras for data acquisition is desirable to simplify and expedite the data analysis process. A technique was developed which will measure fluid velocities with PIV techniques using two successive digital images and two different framing rates simultaneously. The first part of the method will measure changes which occur to the flow field at the relatively slow framing rate of 53.8 ms. The second part will measure changes to the same flow field at the relatively fast framing rate of 100 to 320 {mu}s. The effectiveness of this technique was tested by studying the collapse of steam bubbles in a subcooled tank of water, a relatively high speed phenomena. The tracer particles were recorded and velocity vectors for the fluid were obtained far from the steam bubble collapse.

  11. 2D imaging and 3D sensing data acquisition and mutual registration for painting conservation

    NASA Astrophysics Data System (ADS)

    Fontana, Raffaella; Gambino, Maria Chiara; Greco, Marinella; Marras, Luciano; Pampaloni, Enrico M.; Pelagotti, Anna; Pezzati, Luca; Poggi, Pasquale

    2004-12-01

    We describe the application of 2D and 3D data acquisition and mutual registration to the conservation of paintings. RGB color image acquisition, IR and UV fluorescence imaging, together with the more recent hyperspectral imaging (32 bands) are among the most useful techniques in this field. They generally are meant to provide information on the painting materials, on the employed techniques and on the object state of conservation. However, only when the various images are perfectly registered on each other and on the 3D model, no ambiguity is possible and safe conclusions may be drawn. We present the integration of 2D and 3D measurements carried out on two different paintings: "Madonna of the Yarnwinder" by Leonardo da Vinci, and "Portrait of Lionello d'Este", by Pisanello, both painted in the XV century.

  12. 2D imaging and 3D sensing data acquisition and mutual registration for painting conservation

    NASA Astrophysics Data System (ADS)

    Fontana, Raffaella; Gambino, Maria Chiara; Greco, Marinella; Marras, Luciano; Pampaloni, Enrico M.; Pelagotti, Anna; Pezzati, Luca; Poggi, Pasquale

    2005-01-01

    We describe the application of 2D and 3D data acquisition and mutual registration to the conservation of paintings. RGB color image acquisition, IR and UV fluorescence imaging, together with the more recent hyperspectral imaging (32 bands) are among the most useful techniques in this field. They generally are meant to provide information on the painting materials, on the employed techniques and on the object state of conservation. However, only when the various images are perfectly registered on each other and on the 3D model, no ambiguity is possible and safe conclusions may be drawn. We present the integration of 2D and 3D measurements carried out on two different paintings: "Madonna of the Yarnwinder" by Leonardo da Vinci, and "Portrait of Lionello d'Este", by Pisanello, both painted in the XV century.

  13. Computer acquisition of 3D images utilizing dynamic speckles

    NASA Astrophysics Data System (ADS)

    Kamshilin, Alexei A.; Semenov, Dmitry V.; Nippolainen, Ervin; Raita, Erik

    2006-05-01

    We present novel technique for fast non-contact and continuous profile measurements of rough surfaces by use of dynamic speckles. The dynamic speckle pattern is generated when the laser beam scans the surface under study. The most impressive feature of the proposed technique is its ability to work at extremely high scanning speed of hundreds meters per second. The technique is based on the continuous frequency measurements of the light-power modulation after spatial filtering of the scattered light. The complete optical-electronic system was designed and fabricated for fast measurement of the speckles velocity, its recalculation into the distance, and further data acquisition into computer. The measured surface profile is displayed in a PC monitor in real time. The response time of the measuring system is below 1 μs. Important parameters of the system such as accuracy, range of measurements, and spatial resolution are analyzed. Limits of the spatial filtering technique used for continuous tracking of the speckle-pattern velocity are shown. Possible ways of further improvement of the measurements accuracy are demonstrated. Owing to its extremely fast operation, the proposed technique could be applied for online control of the 3D-shape of complex objects (e.g., electronic circuits) during their assembling.

  14. Patient-Adaptive Reconstruction and Acquisition in Dynamic Imaging with Sensitivity Encoding (PARADISE)

    PubMed Central

    Sharif, Behzad; Derbyshire, J. Andrew; Faranesh, Anthony Z.; Bresler, Yoram

    2010-01-01

    MR imaging of the human heart without explicit cardiac synchronization promises to extend the applicability of cardiac MR to a larger patient population and potentially expand its diagnostic capabilities. However, conventional non-gated imaging techniques typically suffer from low image quality or inadequate spatio-temporal resolution and fidelity. Patient-Adaptive Reconstruction and Acquisition in Dynamic Imaging with Sensitivity Encoding (PARADISE) is a highly-accelerated non-gated dynamic imaging method that enables artifact-free imaging with high spatio-temporal resolutions by utilizing novel computational techniques to optimize the imaging process. In addition to using parallel imaging, the method gains acceleration from a physiologically-driven spatio-temporal support model; hence, it is doubly accelerated. The support model is patient-adaptive, i.e., its geometry depends on dynamics of the imaged slice, e.g., subject’s heart-rate and heart location within the slice. The proposed method is also doubly adaptive as it adapts both the acquisition and reconstruction schemes. Based on the theory of time-sequential sampling, the proposed framework explicitly accounts for speed limitations of gradient encoding and provides performance guarantees on achievable image quality. The presented in-vivo results demonstrate the effectiveness and feasibility of the PARADISE method for high resolution non-gated cardiac MRI during a short breath-hold. PMID:20665794

  15. Machine Learning and Computer Vision System for Phenotype Data Acquisition and Analysis in Plants.

    PubMed

    Navarro, Pedro J; Pérez, Fernando; Weiss, Julia; Egea-Cortines, Marcos

    2016-05-05

    Phenomics is a technology-driven approach with promising future to obtain unbiased data of biological systems. Image acquisition is relatively simple. However data handling and analysis are not as developed compared to the sampling capacities. We present a system based on machine learning (ML) algorithms and computer vision intended to solve the automatic phenotype data analysis in plant material. We developed a growth-chamber able to accommodate species of various sizes. Night image acquisition requires near infrared lightning. For the ML process, we tested three different algorithms: k-nearest neighbour (kNN), Naive Bayes Classifier (NBC), and Support Vector Machine. Each ML algorithm was executed with different kernel functions and they were trained with raw data and two types of data normalisation. Different metrics were computed to determine the optimal configuration of the machine learning algorithms. We obtained a performance of 99.31% in kNN for RGB images and a 99.34% in SVM for NIR. Our results show that ML techniques can speed up phenomic data analysis. Furthermore, both RGB and NIR images can be segmented successfully but may require different ML algorithms for segmentation.

  16. Machine Learning and Computer Vision System for Phenotype Data Acquisition and Analysis in Plants

    PubMed Central

    Navarro, Pedro J.; Pérez, Fernando; Weiss, Julia; Egea-Cortines, Marcos

    2016-01-01

    Phenomics is a technology-driven approach with promising future to obtain unbiased data of biological systems. Image acquisition is relatively simple. However data handling and analysis are not as developed compared to the sampling capacities. We present a system based on machine learning (ML) algorithms and computer vision intended to solve the automatic phenotype data analysis in plant material. We developed a growth-chamber able to accommodate species of various sizes. Night image acquisition requires near infrared lightning. For the ML process, we tested three different algorithms: k-nearest neighbour (kNN), Naive Bayes Classifier (NBC), and Support Vector Machine. Each ML algorithm was executed with different kernel functions and they were trained with raw data and two types of data normalisation. Different metrics were computed to determine the optimal configuration of the machine learning algorithms. We obtained a performance of 99.31% in kNN for RGB images and a 99.34% in SVM for NIR. Our results show that ML techniques can speed up phenomic data analysis. Furthermore, both RGB and NIR images can be segmented successfully but may require different ML algorithms for segmentation. PMID:27164103

  17. Digital Image Analysis of Cereals

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Image analysis is the extraction of meaningful information from images, mainly digital images by means of digital processing techniques. The field was established in the 1950s and coincides with the advent of computer technology, as image analysis is profoundly reliant on computer processing. As t...

  18. Micro-MRI-based image acquisition and processing system for assessing the response to therapeutic intervention

    NASA Astrophysics Data System (ADS)

    Vasilić, B.; Ladinsky, G. A.; Saha, P. K.; Wehrli, F. W.

    2006-03-01

    Osteoporosis is the cause of over 1.5 million bone fractures annually. Most of these fractures occur in sites rich in trabecular bone, a complex network of bony struts and plates found throughout the skeleton. The three-dimensional structure of the trabecular bone network significantly determines mechanical strength and thus fracture resistance. Here we present a data acquisition and processing system that allows efficient noninvasive assessment of trabecular bone structure through a "virtual bone biopsy". High-resolution MR images are acquired from which the trabecular bone network is extracted by estimating the partial bone occupancy of each voxel. A heuristic voxel subdivision increases the effective resolution of the bone volume fraction map and serves a basis for subsequent analysis of topological and orientational parameters. Semi-automated registration and segmentation ensure selection of the same anatomical location in subjects imaged at different time points during treatment. It is shown with excerpts from an ongoing clinical study of early post-menopausal women, that significant reduction in network connectivity occurs in the control group while the structural integrity is maintained in the hormone replacement group. The system described should be suited for large-scale studies designed to evaluate the efficacy of therapeutic intervention in subjects with metabolic bone disease.

  19. Medical Image Analysis Facility

    NASA Technical Reports Server (NTRS)

    1978-01-01

    To improve the quality of photos sent to Earth by unmanned spacecraft. NASA's Jet Propulsion Laboratory (JPL) developed a computerized image enhancement process that brings out detail not visible in the basic photo. JPL is now applying this technology to biomedical research in its Medical lrnage Analysis Facility, which employs computer enhancement techniques to analyze x-ray films of internal organs, such as the heart and lung. A major objective is study of the effects of I stress on persons with heart disease. In animal tests, computerized image processing is being used to study coronary artery lesions and the degree to which they reduce arterial blood flow when stress is applied. The photos illustrate the enhancement process. The upper picture is an x-ray photo in which the artery (dotted line) is barely discernible; in the post-enhancement photo at right, the whole artery and the lesions along its wall are clearly visible. The Medical lrnage Analysis Facility offers a faster means of studying the effects of complex coronary lesions in humans, and the research now being conducted on animals is expected to have important application to diagnosis and treatment of human coronary disease. Other uses of the facility's image processing capability include analysis of muscle biopsy and pap smear specimens, and study of the microscopic structure of fibroprotein in the human lung. Working with JPL on experiments are NASA's Ames Research Center, the University of Southern California School of Medicine, and Rancho Los Amigos Hospital, Downey, California.

  20. Improvement of web-based data acquisition and management system for GOSAT validation lidar data analysis

    NASA Astrophysics Data System (ADS)

    Okumura, Hiroshi; Takubo, Shoichiro; Kawasaki, Takeru; Abdullah, Indra Nugraha; Uchino, Osamu; Morino, Isamu; Yokota, Tatsuya; Nagai, Tomohiro; Sakai, Tetsu; Maki, Takashi; Arai, Kohei

    2013-01-01

    A web-base data acquisition and management system for GOSAT (Greenhouse gases Observation SATellite) validation lidar data-analysis has been developed. The system consists of data acquisition sub-system (DAS) and data management sub-system (DMS). DAS written in Perl language acquires AMeDAS (Automated Meteorological Data Acquisition System) ground-level local meteorological data, GPS Radiosonde upper-air meteorological data, ground-level oxidant data, skyradiometer data, skyview camera images, meteorological satellite IR image data and GOSAT validation lidar data. DMS written in PHP language demonstrates satellite-pass date and all acquired data. In this article, we briefly describe some improvement for higher performance and higher data usability. GPS Radiosonde upper-air meteorological data and U.S. standard atmospheric model in DAS automatically calculate molecule number density profiles. Predicted ozone density prole images above Saga city are also calculated by using Meteorological Research Institute (MRI) chemistry-climate model version 2 for comparison to actual ozone DIAL data.

  1. Insurance Contract Analysis for Company Decision Support in Acquisition Management

    NASA Astrophysics Data System (ADS)

    Chernovita, H. P.; Manongga, D.; Iriani, A.

    2017-01-01

    One of company activities to retain their business is marketing the products which include in acquisition management to get new customers. Insurance contract analysis using ID3 to produce decision tree and rules to be decision support for the insurance company. The decision tree shows 13 rules that lead to contract termination claim. This could be a guide for the insurance company in acquisition management to prevent contract binding with these contract condition because it has a big chance for the customer to terminate their insurance contract before its expired date. As the result, there are several strong points that could be the determinant of contract termination such as: 1) customer age whether too young or too old, 2) long insurance period (above 10 years), 3) big insurance amount, 4) big amount of premium charges, and 5) payment method.

  2. Temporal optimisation of image acquisition for land cover classification with Random Forest and MODIS time-series

    NASA Astrophysics Data System (ADS)

    Nitze, Ingmar; Barrett, Brian; Cawkwell, Fiona

    2015-02-01

    The analysis and classification of land cover is one of the principal applications in terrestrial remote sensing. Due to the seasonal variability of different vegetation types and land surface characteristics, the ability to discriminate land cover types changes over time. Multi-temporal classification can help to improve the classification accuracies, but different constraints, such as financial restrictions or atmospheric conditions, may impede their application. The optimisation of image acquisition timing and frequencies can help to increase the effectiveness of the classification process. For this purpose, the Feature Importance (FI) measure of the state-of-the art machine learning method Random Forest was used to determine the optimal image acquisition periods for a general (Grassland, Forest, Water, Settlement, Peatland) and Grassland specific (Improved Grassland, Semi-Improved Grassland) land cover classification in central Ireland based on a 9-year time-series of MODIS Terra 16 day composite data (MOD13Q1). Feature Importances for each acquisition period of the Enhanced Vegetation Index (EVI) and Normalised Difference Vegetation Index (NDVI) were calculated for both classification scenarios. In the general land cover classification, the months December and January showed the highest, and July and August the lowest separability for both VIs over the entire nine-year period. This temporal separability was reflected in the classification accuracies, where the optimal choice of image dates outperformed the worst image date by 13% using NDVI and 5% using EVI on a mono-temporal analysis. With the addition of the next best image periods to the data input the classification accuracies converged quickly to their limit at around 8-10 images. The binary classification schemes, using two classes only, showed a stronger seasonal dependency with a higher intra-annual, but lower inter-annual variation. Nonetheless anomalous weather conditions, such as the cold winter of

  3. Magnetic resonance imaging acquisition techniques intended to decrease movement artefact in paediatric brain imaging: a systematic review.

    PubMed

    Woodfield, Julie; Kealey, Susan

    2015-08-01

    Attaining paediatric brain images of diagnostic quality can be difficult because of young age or neurological impairment. The use of anaesthesia to reduce movement in MRI increases clinical risk and cost, while CT, though faster, exposes children to potentially harmful ionising radiation. MRI acquisition techniques that aim to decrease movement artefact may allow diagnostic paediatric brain imaging without sedation or anaesthesia. We conducted a systematic review to establish the evidence base for ultra-fast sequences and sequences using oversampling of k-space in paediatric brain MR imaging. Techniques were assessed for imaging time, occurrence of movement artefact, the need for sedation, and either image quality or diagnostic accuracy. We identified 24 relevant studies. We found that ultra-fast techniques had shorter imaging acquisition times compared to standard MRI. Techniques using oversampling of k-space required equal or longer imaging times than standard MRI. Both ultra-fast sequences and those using oversampling of k-space reduced movement artefact compared with standard MRI in unsedated children. Assessment of overall diagnostic accuracy was difficult because of the heterogeneous patient populations, imaging indications, and reporting methods of the studies. In children with shunt-treated hydrocephalus there is evidence that ultra-fast MRI is sufficient for the assessment of ventricular size.

  4. Pain related inflammation analysis using infrared images

    NASA Astrophysics Data System (ADS)

    Bhowmik, Mrinal Kanti; Bardhan, Shawli; Das, Kakali; Bhattacharjee, Debotosh; Nath, Satyabrata

    2016-05-01

    Medical Infrared Thermography (MIT) offers a potential non-invasive, non-contact and radiation free imaging modality for assessment of abnormal inflammation having pain in the human body. The assessment of inflammation mainly depends on the emission of heat from the skin surface. Arthritis is a disease of joint damage that generates inflammation in one or more anatomical joints of the body. Osteoarthritis (OA) is the most frequent appearing form of arthritis, and rheumatoid arthritis (RA) is the most threatening form of them. In this study, the inflammatory analysis has been performed on the infrared images of patients suffering from RA and OA. For the analysis, a dataset of 30 bilateral knee thermograms has been captured from the patient of RA and OA by following a thermogram acquisition standard. The thermograms are pre-processed, and areas of interest are extracted for further processing. The investigation of the spread of inflammation is performed along with the statistical analysis of the pre-processed thermograms. The objectives of the study include: i) Generation of a novel thermogram acquisition standard for inflammatory pain disease ii) Analysis of the spread of the inflammation related to RA and OA using K-means clustering. iii) First and second order statistical analysis of pre-processed thermograms. The conclusion reflects that, in most of the cases, RA oriented inflammation affects bilateral knees whereas inflammation related to OA present in the unilateral knee. Also due to the spread of inflammation in OA, contralateral asymmetries are detected through the statistical analysis.

  5. Parameter importance in FRAP acquisition and analysis: a simulation approach.

    PubMed

    Mai, Juliane; Trump, Saskia; Lehmann, Irina; Attinger, Sabine

    2013-05-07

    Fluorescence recovery after photobleaching (FRAP) is a widespread technique used to determine intracellular reaction and diffusion parameters. In recent years, due to technical advances and an increasing number of mathematical models for analysis, there was a resurging interest in FRAP applications. However, care has to be taken when inverting parameters from such data. We study potential influences on FRAP acquisition and analysis like initial fluorescence distribution, membrane passage, and geometrical aspects. Monte Carlo simulations are employed for the investigation of reaction-diffusion processes to additionally include cases in which no analytical description is available. To assess the importance of influencing factors we apply a sensitivity method based on elementary effects providing an estimate for the global parameter space. The combination of simulations and sensitivity measure helps us to predict ranges of parameters used in acquisition and analysis for which a reliably inversion of reaction-diffusion parameters is possible. Using this approach, we show that FRAP data are highly susceptible to misinterpretation. However, by identifying the parameters of susceptibility, our analysis provides the means for taking measures to significantly improve FRAP data interpretation and analysis.

  6. Imageability, age of acquisition, and frequency factors in acronym comprehension.

    PubMed

    Playfoot, David; Izura, Cristina

    2013-06-01

    In spite of their unusual orthographic and phonological form, acronyms (e.g., BBC, HIV, NATO) can become familiar to the reader, and their meaning can be accessed well enough that they are understood. The factors in semantic access for acronym stimuli were assessed using a word association task. Two analyses examined the time taken to generate a word association response to acronym cues. Responses were recorded more quickly to cues that elicited a large proportion of semantic responses, and those that were high in associative strength. Participants were shown to be faster to respond to cues which were imageable or early acquired. Frequency was not a significant predictor of word association responses. Implications for theories of lexical organisation are discussed.

  7. New developments in electron microscopy for serial image acquisition of neuronal profiles.

    PubMed

    Kubota, Yoshiyuki

    2015-02-01

    Recent developments in electron microscopy largely automate the continuous acquisition of serial electron micrographs (EMGs), previously achieved by laborious manual serial ultrathin sectioning using an ultramicrotome and ultrastructural image capture process with transmission electron microscopy. The new systems cut thin sections and capture serial EMGs automatically, allowing for acquisition of large data sets in a reasonably short time. The new methods are focused ion beam/scanning electron microscopy, ultramicrotome/serial block-face scanning electron microscopy, automated tape-collection ultramicrotome/scanning electron microscopy and transmission electron microscope camera array. In this review, their positive and negative aspects are discussed.

  8. Multiple-CCD stereo acquisition system for high-speed imaging

    NASA Astrophysics Data System (ADS)

    Yafuso, Eiji S.; Sass, David T.; Dereniak, Eustace L.; Hoffman, Steven; Gonzalez, Rene; Gonzalez, Martin; Rettke, Douglas

    1998-10-01

    A high-speed 3D imaging system has been developed using multiple independent CCD cameras with sequentially triggered acquisition and individual field storage capability. The system described here utilizes sixteen independent cameras. A stereo alignment and triggering scheme arranges the cameras into two angularly separated banks of eight cameras each. By simultaneously triggering correlated stereo pairs, an eight-frame sequence of stereo images is captured. The delays can be individually adjusted to yield a greater number of acquired frames during more rapid segments of the vent, and the individual integration periods may be adjusted to ensure adequate radiometric response while minimizing image blur. Representation of the data as a 3D sequence introduces the issue of independent camera coordinate registration with the real scene. A discussion of the forward and inverse transform operator for the digital data is provided along with a description of the acquisition system.

  9. Automated system for acquisition and image processing for the control and monitoring boned nopal

    NASA Astrophysics Data System (ADS)

    Luevano, E.; de Posada, E.; Arronte, M.; Ponce, L.; Flores, T.

    2013-11-01

    This paper describes the design and fabrication of a system for acquisition and image processing to control the removal of thorns nopal vegetable (Opuntia ficus indica) in an automated machine that uses pulses of a laser of Nd: YAG. The areolas, areas where thorns grow on the bark of the Nopal, are located applying segmentation algorithms to the images obtained by a CCD. Once the position of the areolas is known, coordinates are sent to a motors system that controls the laser to interact with all areolas and remove the thorns of the nopal. The electronic system comprises a video decoder, memory for image and software storage, and digital signal processor for system control. The firmware programmed tasks on acquisition, preprocessing, segmentation, recognition and interpretation of the areolas. This system achievement identifying areolas and generating table of coordinates of them, which will be send the motor galvo system that controls the laser for removal

  10. Whole Heart Coronary Imaging with Flexible Acquisition Window and Trigger Delay

    PubMed Central

    Kawaji, Keigo; Foppa, Murilo; Roujol, Sébastien; Akçakaya, Mehmet; Nezafat, Reza

    2015-01-01

    Coronary magnetic resonance imaging (MRI) requires a correctly timed trigger delay derived from a scout cine scan to synchronize k-space acquisition with the quiescent period of the cardiac cycle. However, heart rate changes between breath-held cine and free-breathing coronary imaging may result in inaccurate timing errors. Additionally, the determined trigger delay may not reflect the period of minimal motion for both left and right coronary arteries or different segments. In this work, we present a whole-heart coronary imaging approach that allows flexible selection of the trigger delay timings by performing k-space sampling over an enlarged acquisition window. Our approach addresses coronary motion in an interactive manner by allowing the operator to determine the temporal window with minimal cardiac motion for each artery region. An electrocardiogram-gated, k-space segmented 3D radial stack-of-stars sequence that employs a custom rotation angle is developed. An interactive reconstruction and visualization platform is then employed to determine the subset of the enlarged acquisition window for minimal coronary motion. Coronary MRI was acquired on eight healthy subjects (5 male, mean age = 37 ± 18 years), where an enlarged acquisition window of 166–220 ms was set 50 ms prior to the scout-derived trigger delay. Coronary visualization and sharpness scores were compared between the standard 120 ms window set at the trigger delay, and those reconstructed using a manually adjusted window. The proposed method using manual adjustment was able to recover delineation of five mid and distal right coronary artery regions that were otherwise not visible from the standard window, and the sharpness scores improved in all coronary regions using the proposed method. This paper demonstrates the feasibility of a whole-heart coronary imaging approach that allows interactive selection of any subset of the enlarged acquisition window for a tailored reconstruction for each branch

  11. Whole heart coronary imaging with flexible acquisition window and trigger delay.

    PubMed

    Kawaji, Keigo; Foppa, Murilo; Roujol, Sébastien; Akçakaya, Mehmet; Nezafat, Reza

    2015-01-01

    Coronary magnetic resonance imaging (MRI) requires a correctly timed trigger delay derived from a scout cine scan to synchronize k-space acquisition with the quiescent period of the cardiac cycle. However, heart rate changes between breath-held cine and free-breathing coronary imaging may result in inaccurate timing errors. Additionally, the determined trigger delay may not reflect the period of minimal motion for both left and right coronary arteries or different segments. In this work, we present a whole-heart coronary imaging approach that allows flexible selection of the trigger delay timings by performing k-space sampling over an enlarged acquisition window. Our approach addresses coronary motion in an interactive manner by allowing the operator to determine the temporal window with minimal cardiac motion for each artery region. An electrocardiogram-gated, k-space segmented 3D radial stack-of-stars sequence that employs a custom rotation angle is developed. An interactive reconstruction and visualization platform is then employed to determine the subset of the enlarged acquisition window for minimal coronary motion. Coronary MRI was acquired on eight healthy subjects (5 male, mean age = 37 ± 18 years), where an enlarged acquisition window of 166-220 ms was set 50 ms prior to the scout-derived trigger delay. Coronary visualization and sharpness scores were compared between the standard 120 ms window set at the trigger delay, and those reconstructed using a manually adjusted window. The proposed method using manual adjustment was able to recover delineation of five mid and distal right coronary artery regions that were otherwise not visible from the standard window, and the sharpness scores improved in all coronary regions using the proposed method. This paper demonstrates the feasibility of a whole-heart coronary imaging approach that allows interactive selection of any subset of the enlarged acquisition window for a tailored reconstruction for each branch

  12. Foil-hole and data image quality assessment in 3DEM: Towards high-throughput image acquisition in the electron microscope.

    PubMed

    Vargas, J; Franken, E; Sorzano, C O S; Gomez-Blanco, J; Schoenmakers, R; Koster, A J; Carazo, J M

    2016-12-01

    Automatic or semiautomatic data collection approaches on a transmission electron microscope (TEM) for Single Particle Analysis, capable of acquiring large datasets composed of only high quality images, are of great importance to obtain 3D density maps with the highest resolution possible. Typically, this task is performed by an experienced microscopist, who manually decides to keep or discard images according to subjective criteria. Therefore, this methodology is slow, intensive in human work and subjective. In this work, we propose a method to automatically or semiautomatically perform this image selection task. The approach is based on some simple, fast and effective image quality descriptors, which can be computed during acquisition, to characterize foil-hole and data images. The proposed approach has been used to evaluate the quality of different datasets consisting of foil-hole and data images obtained with a FEI Titan Krios electron microscope. The results show that the proposed method is very effective evaluating the quality of foil-hole and data images, as well as predicting the quality of the data images from the foil-hole images.

  13. A review of breast tomosynthesis. Part I. The image acquisition process

    SciTech Connect

    Sechopoulos, Ioannis

    2013-01-15

    Mammography is a very well-established imaging modality for the early detection and diagnosis of breast cancer. However, since the introduction of digital imaging to the realm of radiology, more advanced, and especially tomographic imaging methods have been made possible. One of these methods, breast tomosynthesis, has finally been introduced to the clinic for routine everyday use, with potential to in the future replace mammography for screening for breast cancer. In this two part paper, the extensive research performed during the development of breast tomosynthesis is reviewed, with a focus on the research addressing the medical physics aspects of this imaging modality. This first paper will review the research performed on the issues relevant to the image acquisition process, including system design, optimization of geometry and technique, x-ray scatter, and radiation dose. The companion to this paper will review all other aspects of breast tomosynthesis imaging, including the reconstruction process.

  14. Interactive Image Analysis System Design,

    DTIC Science & Technology

    1982-12-01

    This report describes a design for an interactive image analysis system (IIAS), which implements terrain data extraction techniques. The design... analysis system. Additionally, the system is fully capable of supporting many generic types of image analysis and data processing, and is modularly...employs commercially available, state of the art minicomputers and image display devices with proven software to achieve a cost effective, reliable image

  15. Parallel Algorithms for Image Analysis.

    DTIC Science & Technology

    1982-06-01

    8217 _ _ _ _ _ _ _ 4. TITLE (aid Subtitle) S. TYPE OF REPORT & PERIOD COVERED PARALLEL ALGORITHMS FOR IMAGE ANALYSIS TECHNICAL 6. PERFORMING O4G. REPORT NUMBER TR-1180...Continue on reverse side it neceesary aid Identlfy by block number) Image processing; image analysis ; parallel processing; cellular computers. 20... IMAGE ANALYSIS TECHNICAL 6. PERFORMING ONG. REPORT NUMBER TR-1180 - 7. AUTHOR(&) S. CONTRACT OR GRANT NUMBER(s) Azriel Rosenfeld AFOSR-77-3271 9

  16. Live dynamic OCT imaging of cardiac structure and function in mouse embryos with 43 Hz direct volumetric data acquisition

    NASA Astrophysics Data System (ADS)

    Wang, Shang; Singh, Manmohan; Lopez, Andrew L.; Wu, Chen; Raghunathan, Raksha; Schill, Alexander; Li, Jiasong; Larin, Kirill V.; Larina, Irina V.

    2016-03-01

    Efficient phenotyping of cardiac dynamics in live mouse embryos has significant implications on understanding of early mammalian heart development and congenital cardiac defects. Recent studies established optical coherence tomography (OCT) as a powerful tool for live embryonic heart imaging in various animal models. However, current four-dimensional (4D) OCT imaging of the beating embryonic heart largely relies on gated data acquisition or postacquisition synchronization, which brings errors when cardiac cycles lack perfect periodicity and is time consuming and computationally expensive. Here, we report direct 4D OCT imaging of the structure and function of cardiac dynamics in live mouse embryos achieved by employing a Fourier domain mode-locking swept laser source that enables ~1.5 MHz A-line rate. Through utilizing both forward and backward scans of a resonant mirror, we obtained a ~6.4 kHz frame rate, which allows for a direct volumetric data acquisition speed of ~43 Hz, around 20 times of the early-stage mouse embryonic heart rate. Our experiments were performed on mouse embryos at embryonic day 9.5. Time-resolved 3D cardiodynamics clearly shows the heart structure in motion. We present analysis of cardiac wall movement and its velocity from the primitive atrium and ventricle. Our results suggest that the combination of ultrahigh-speed OCT imaging with live embryo culture could be a useful embryonic heart phenotyping approach for mouse mutants modeling human congenital heart diseases.

  17. A novel acquisition-reconstruction algorithm for surface magnetic resonance imaging.

    PubMed

    Franchi, Danilo; Sotgiu, Antonello; Placidi, Giuseppe

    2008-11-01

    In U-shaped, hand-size magnetic resonance surface scanners, imaging is performed along only one spatial direction, with the application of just one gradient (one-dimensional imaging). Lateral spatial resolution can be obtained by magnet displacement, but, in this case, resolution is very poor (on the order of some millimeters) and cannot be useful for high-resolution imaging applications. In this article, an innovative technique for acquisition and reconstruction of images produced by U-shaped, hand-size MRI surface scanners is presented. The proposed method is based on the acquisition of overlapping strips and an analytical reconstruction technique; it is capable of arbitrarily improving spatial lateral resolution without either using a second magnetic field gradient or making any assumptions about the imaged sample extension. Numerical simulations on synthetic images are reported demonstrating the method functionalities. The presented method also makes it possible to use U-shaped, hand-size MRI surface scanners for high-resolution biomedical applications, such as the imaging of skin lesions.

  18. Non-Equispaced System Matrix Acquisition for Magnetic Particle Imaging Based on Lissajous Node Points.

    PubMed

    Kaethner, Christian; Erb, Wolfgang; Ahlborg, Mandy; Szwargulski, Patryk; Knopp, Tobias; Buzug, Thorsten M

    2016-11-01

    Magnetic Particle Imaging (MPI) is an emerging technology in the field of (pre)clinical imaging. The acquisition of a particle signal is realized along specific sampling trajectories covering a defined field of view (FOV). In a system matrix (SM) based reconstruction procedure, the commonly used acquisition path in MPI is a Lissajous trajectory. Such a trajectory features an inhomogeneous coverage of the FOV, i.e. the center region is sampled less dense than the regions towards the edges of the FOV. Conventionally, the respective SM acquisition and the subsequent reconstruction do not reflect this inhomogeneous coverage. Instead, they are performed on an equispaced grid. The objective of this work is to introduce a sampling grid that inherently features the aforementioned inhomogeneity by using node points of Lissajous trajectories. Paired with a tailored polynomial interpolation of the reconstructed MPI signal, the entire image can be recovered. It is the first time that such a trajectory related non-equispaced grid is used for image reconstruction on simulated and measured MPI data and it is shown that the number of sampling positions can be reduced, while the spatial resolution remains constant.

  19. A VAX and PC-based data acquisition system for MCA, scanning and list-mode analysis

    NASA Astrophysics Data System (ADS)

    Churms, C. L.; Pilcher, J. V.; Springhorn, K. A.; Tapper, U. A. S.

    1993-05-01

    The design of the data acquisition system for the National Accelerator Centre's nuclear microprobe is described. The system consists of an intelligent PC scanner and imaging terminal, interfaced to a VAX-11/750 running a general purpose data acquisition package XSYS. In this way, the user friendliness of a dedicated, intelligent scanner has been combined with the flexibility of a general purpose data acquisition package. It is illustrated how simple tasks such as beam focussing, area of interest localization, and quantitative single spot PIXE or RBS analysis can be performed with minimal complexity, whereas requirements of a more complex nature can be implemented routinely using the general purpose acquisition package with standard command files. Even unique studies can be performed by means of minor software modifications.

  20. Brain Imaging Analysis

    PubMed Central

    BOWMAN, F. DUBOIS

    2014-01-01

    The increasing availability of brain imaging technologies has led to intense neuroscientific inquiry into the human brain. Studies often investigate brain function related to emotion, cognition, language, memory, and numerous other externally induced stimuli as well as resting-state brain function. Studies also use brain imaging in an attempt to determine the functional or structural basis for psychiatric or neurological disorders and, with respect to brain function, to further examine the responses of these disorders to treatment. Neuroimaging is a highly interdisciplinary field, and statistics plays a critical role in establishing rigorous methods to extract information and to quantify evidence for formal inferences. Neuroimaging data present numerous challenges for statistical analysis, including the vast amounts of data collected from each individual and the complex temporal and spatial dependence present. We briefly provide background on various types of neuroimaging data and analysis objectives that are commonly targeted in the field. We present a survey of existing methods targeting these objectives and identify particular areas offering opportunities for future statistical contribution. PMID:25309940

  1. Performance of a Novel PMMA Polymer Imaging Bundle for Field Acquisition and Wavefront Sensing

    NASA Astrophysics Data System (ADS)

    Richards, S. N.; Leon-Saval, S.; Goodwin, M.; Zheng, J.; Lawrence, J. S.; Bryant, J. J.; Bland-Hawthorn, J.; Norris, B.; Cvetojevic, N.; Argyros, A.

    2017-01-01

    Imaging bundles provide a convenient way to translate a spatially coherent image, yet conventional imaging bundles made from silica fibre optics typically remain expensive with large losses due to poor filling factors ( 40%). We present the characterisation of a novel polymer imaging bundle made from poly(methyl methacrylate) (PMMA) that is considerably cheaper and a better alternative to silica imaging bundles over short distances ( 1 m; from the middle to the edge of a telescope's focal plane). The large increase in filling factor (92% for the polymer imaging bundle) outweighs the large increase in optical attenuation from using PMMA (1 dB/m) instead of silica (10-3 dB/m). We present and discuss current and possible future multi-object applications of the polymer imaging bundle in the context of astronomical instrumentation including: field acquisition, guiding, wavefront sensing, narrow-band imaging, aperture masking, and speckle imaging. The use of PMMA limits its use in low-light applications (e.g., imaging of galaxies); however, it is possible to fabricate polymer imaging bundles from a range of polymers that are better suited to the desired science.

  2. A flexible high-rate USB2 data acquisition system for PET and SPECT imaging

    SciTech Connect

    J. Proffitt, W. Hammond, S. Majewski, V. Popov, R.R. Raylman, A.G. Weisenberger, R. Wojcik

    2006-02-01

    A new flexible data acquisition system has been developed to instrument gamma-ray imaging detectors designed by the Jefferson Lab Detector and Imaging Group. Hardware consists of 16-channel data acquisition modules installed on USB2 carrier boards. Carriers have been designed to accept one, two, and four modules. Application trigger rate and channel density determines the number of acquisition boards and readout computers used. Each channel has an independent trigger, gated integrator and a 2.5 MHz 12-bit ADC. Each module has an FPGA for analog control and signal processing. Processing includes a 5 ns 40-bit trigger time stamp and programmable triggering, gating, ADC timing, offset and gain correction, charge and pulse-width discrimination, sparsification, event counting, and event assembly. The carrier manages global triggering and transfers module data to a USB buffer. High-granularity time-stamped triggering is suitable for modular detectors. Time stamped events permit dynamic studies, complex offline event assembly, and high-rate distributed data acquisition. A sustained USB data rate of 20 Mbytes/s, a sustained trigger rate of 300 kHz for 32 channels, and a peak trigger rate of 2.5 MHz to FIFO memory were achieved. Different trigger, gating, processing, and event assembly techniques were explored. Target applications include >100 kHz coincidence rate PET detectors, dynamic SPECT detectors, miniature and portable gamma detectors for small-animal and clinical use.

  3. DIDA - Dynamic Image Disparity Analysis.

    DTIC Science & Technology

    1982-12-31

    Understanding, Dynamic Image Analysis , Disparity Analysis, Optical Flow, Real-Time Processing ___ 20. ABSTRACT (Continue on revere side If necessary aid identify...three aspects of dynamic image analysis must be studied: effectiveness, generality, and efficiency. In addition, efforts must be made to understand the...environment. A better understanding of the need for these Limiting constraints is required. Efficiency is obviously important if dynamic image analysis is

  4. Robust Depth Image Acquisition Using Modulated Pattern Projection and Probabilistic Graphical Models

    PubMed Central

    Kravanja, Jaka; Žganec, Mario; Žganec-Gros, Jerneja; Dobrišek, Simon; Štruc, Vitomir

    2016-01-01

    Depth image acquisition with structured light approaches in outdoor environments is a challenging problem due to external factors, such as ambient sunlight, which commonly affect the acquisition procedure. This paper presents a novel structured light sensor designed specifically for operation in outdoor environments. The sensor exploits a modulated sequence of structured light projected onto the target scene to counteract environmental factors and estimate a spatial distortion map in a robust manner. The correspondence between the projected pattern and the estimated distortion map is then established using a probabilistic framework based on graphical models. Finally, the depth image of the target scene is reconstructed using a number of reference frames recorded during the calibration process. We evaluate the proposed sensor on experimental data in indoor and outdoor environments and present comparative experiments with other existing methods, as well as commercial sensors. PMID:27775570

  5. Robust Depth Image Acquisition Using Modulated Pattern Projection and Probabilistic Graphical Models.

    PubMed

    Kravanja, Jaka; Žganec, Mario; Žganec-Gros, Jerneja; Dobrišek, Simon; Štruc, Vitomir

    2016-10-19

    Depth image acquisition with structured light approaches in outdoor environments is a challenging problem due to external factors, such as ambient sunlight, which commonly affect the acquisition procedure. This paper presents a novel structured light sensor designed specifically for operation in outdoor environments. The sensor exploits a modulated sequence of structured light projected onto the target scene to counteract environmental factors and estimate a spatial distortion map in a robust manner. The correspondence between the projected pattern and the estimated distortion map is then established using a probabilistic framework based on graphical models. Finally, the depth image of the target scene is reconstructed using a number of reference frames recorded during the calibration process. We evaluate the proposed sensor on experimental data in indoor and outdoor environments and present comparative experiments with other existing methods, as well as commercial sensors.

  6. Isolated sixth cranial nerve aplasia visualized with Fast Imaging Employing Steady-State Acquisition (FIESTA) MRI.

    PubMed

    Pilyugina, Svetlana A; Fischbein, Nancy J; Liao, Y Joyce; McCulley, Timothy J

    2007-06-01

    An otherwise healthy 12-month-old girl presented for evaluation of reduced abduction of the left eye detected at 6 months of age. The remainder of the examination was unremarkable. A special MRI sequence-fast imaging employing steady-state acquisition (FIESTA)-visualized the right but not the left sixth nerve cisternal segment. This is the first reported use of the MRI FIESTA sequence to diagnose aplasia of the sixth cranial nerve.

  7. Knowledge Acquisition, Validation, and Maintenance in a Planning System for Automated Image Processing

    NASA Technical Reports Server (NTRS)

    Chien, Steve A.

    1996-01-01

    A key obstacle hampering fielding of AI planning applications is the considerable expense of developing, verifying, updating, and maintainting the planning knowledge base (KB). Planning systems must be able to compare favorably in terms of software lifecycle costs to other means of automation such as scripts or rule-based expert systems. This paper describes a planning application of automated imaging processing and our overall approach to knowledge acquisition for this application.

  8. A flexible Labview{trademark}-based data acquisition and analysis system for scanning microscopy

    SciTech Connect

    Daniel H. Morse; Arlyn J. Antolak; Graham S. Bench; Mark L. Roberts

    1998-10-08

    A new data analysis system has been developed with computer-controlled beam and sample positioning, video sample imaging, multiple large solid angle detectors for x-rays and gamma-rays, and surface barrier detectors for charged particles. The system uses the LabVIEW{trademark} programming language allowing it to be easily ported between different computer operating systems. In the present configuration, digital signal processors are directly interfaced to a SCSI CAMAC controller. However, the modular software design permits the substitution of other hardware with LabVIEW-supported drivers. On-line displays of histogram and two-dimensional elemental map images provide a user-friendly data acquisition interface. Subregions of the two-dimensional maps may be selected interactively for detailed analysis or for subsequent scanning. Off-line data processing of archived data currently yields elemental maps, analyzed spectra and reconstructions of tomographic data.

  9. Improving the spatial resolution of magnetic resonance inverse imaging via the blipped-CAIPI acquisition scheme.

    PubMed

    Chang, Wei-Tang; Setsompop, Kawin; Ahveninen, Jyrki; Belliveau, John W; Witzel, Thomas; Lin, Fa-Hsuan

    2014-05-01

    Using simultaneous acquisition from multiple channels of a radio-frequency (RF) coil array, magnetic resonance inverse imaging (InI) achieves functional MRI acquisitions at a rate of 100ms per whole-brain volume. InI accelerates the scan by leaving out partition encoding steps and reconstructs images by solving under-determined inverse problems using RF coil sensitivity information. Hence, the correlated spatial information available in the coil array causes spatial blurring in the InI reconstruction. Here, we propose a method that employs gradient blips in the partition encoding direction during the acquisition to provide extra spatial encoding in order to better differentiate signals from different partitions. According to our simulations, this blipped-InI (bInI) method can increase the average spatial resolution by 15.1% (1.3mm) across the whole brain and from 32.6% (4.2mm) in subcortical regions, as compared to the InI method. In a visual fMRI experiment, we demonstrate that, compared to InI, the spatial distribution of bInI BOLD response is more consistent with that of a conventional echo-planar imaging (EPI) at the level of individual subjects. With the improved spatial resolution, especially in subcortical regions, bInI can be a useful fMRI tool for obtaining high spatiotemporal information for clinical and cognitive neuroscience studies.

  10. An approach to automated acquisition of cryoEM images from lacey carbon grids.

    PubMed

    Nicholson, William V; White, Howard; Trinick, John

    2010-12-01

    An approach to automated acquisition of cryoEM image data from lacey carbon grids using the Leginon program is described. Automated liquid nitrogen top up of the specimen holder dewar was used as a step towards full automation, without operator intervention during the course of data collection. During cryoEM studies of actin labelled with myosin V, we have found it necessary to work with lacey grids rather than Quantifoil or C-flat grids due to interaction of myosin V with the support film. Lacey grids have irregular holes of variable shape and size, in contrast to Quantifoil or C-flat grids which have a regular array of similar circular holes on each grid square. Other laboratories also prefer to work with grids with irregular holes for a variety of reasons. Therefore, it was necessary to develop a different strategy from normal Leginon usage for working with lacey grids for targeting holes for image acquisition and suitable areas for focussing prior to image acquisition. This approach was implemented by using the extensible framework provided by Leginon and by developing a new MSI application within that framework which includes a new Leginon node (for a novel method for finding focus targets).

  11. Improving the spatial resolution of Magnetic Resonance Inverse Imaging via the blipped-CAIPI acquisition scheme

    PubMed Central

    Chang, Wei-Tang; Setsompop, Kawin; Ahveninen, Jyrki; Belliveau, John W.; Witzel, Thomas; Lin, Fa-Hsuan

    2014-01-01

    Using simultaneous acquisition from multiple channels of a radio-frequency (RF) coil array, magnetic resonance inverse imaging (InI) achieves functional MRI acquisitions at a rate of 100 ms per whole-brain volume. InI accelerates the scan by leaving out partition encoding steps and reconstructs images by solving under-determined inverse problems using RF coil sensitivity information. Hence, the correlated spatial information available in the coil array causes spatial blurring in the InI reconstruction. Here, we propose a method that employs gradient blips in the partition encoding direction during the acquisition to provide extra spatial encoding in order to better differentiate signals from different partitions. According to our simulations, this blipped-InI (bInI) method can increase the average spatial resolution by 15.1% (1.3 mm) across the whole brain and from 32.6% (4.2 mm) in subcortical regions, as compared to the InI method. In a visual fMRI experiment, we demonstrate that, compared to InI, the spatial distribution of bInI BOLD response is more consistent with that of a conventional echo-planar imaging (EPI) at the level of individual subjects. With the improved spatial resolution, especially in subcortical regions, bInI can be a useful fMRI tool for obtaining high spatiotemporal information for clinical and cognitive neuroscience studies. PMID:24374076

  12. High-Speed MALDI-TOF Imaging Mass Spectrometry: Rapid Ion Image Acquisition and Considerations for Next Generation Instrumentation

    PubMed Central

    Spraggins, Jeffrey M.; Caprioli, Richard M.

    2012-01-01

    A prototype matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer has been used for high-speed ion image acquisition. The instrument incorporates a Nd:YLF solid state laser capable of pulse repetition rates up to 5 kHz and continuous laser raster sampling for high-throughput data collection. Lipid ion images of a sagittal rat brain tissue section were collected in 10 min with an effective acquisition rate of roughly 30 pixels/s. These results represent more than a 10-fold increase in throughput compared with current commercially available instrumentation. Experiments aimed at improving conditions for continuous laser raster sampling for imaging are reported, highlighting proper laser repetition rates and stage velocities to avoid signal degradation from significant oversampling. As new high spatial resolution and large sample area applications present themselves, the development of high-speed microprobe MALDI imaging mass spectrometry is essential to meet the needs of those seeking new technologies for rapid molecular imaging. PMID:21953043

  13. A comparison of peripheral imaging technologies for bone and muscle quantification: a technical review of image acquisition.

    PubMed

    Wong, A K

    2016-12-14

    The choice of an appropriate imaging technique to quantify bone, muscle, or muscle adiposity needs to be guided by a thorough understanding of its competitive advantages over other modalities balanced by its limitations. This review details the technical machinery and methods behind peripheral quantitative computed tomography (pQCT), high-resolution (HR)-pQCT, and magnetic resonance imaging (MRI) that drive successful depiction of bone and muscle morphometry, densitometry, and structure. It discusses a number of image acquisition settings, the challenges associated with using one versus another, and compares the risk-benefits across the different modalities. Issues related to all modalities including partial volume artifact, beam hardening, calibration, and motion assessment are also detailed. The review further provides data and images to illustrate differences between methods to better guide the reader in selecting an imaging method strategically. Overall, investigators should be cautious of the impact of imaging parameters on image signal or contrast-to-noise-ratios, and the need to report these settings in future publications. The effect of motion should be assessed on images and a decision made to exclude prior to segmentation. A more standardized approach to imaging bone and muscle on pQCT and MRI could enhance comparability across studies and could improve the quality of meta-analyses.

  14. A comparison of peripheral imaging technologies for bone and muscle quantification: a technical review of image acquisition

    PubMed Central

    Wong, A.K.O.

    2016-01-01

    The choice of an appropriate imaging technique to quantify bone, muscle, or muscle adiposity needs to be guided by a thorough understanding of its competitive advantages over other modalities balanced by its limitations. This review details the technical machinery and methods behind peripheral quantitative computed tomography (pQCT), high-resolution (HR)-pQCT, and magnetic resonance imaging (MRI) that drive successful depiction of bone and muscle morphometry, densitometry, and structure. It discusses a number of image acquisition settings, the challenges associated with using one versus another, and compares the risk-benefits across the different modalities. Issues related to all modalities including partial volume artifact, beam hardening, calibration, and motion assessment are also detailed. The review further provides data and images to illustrate differences between methods to better guide the reader in selecting an imaging method strategically. Overall, investigators should be cautious of the impact of imaging parameters on image signal or contrast-to-noise-ratios, and the need to report these settings in future publications. The effect of motion should be assessed on images and a decision made to exclude prior to segmentation. A more standardized approach to imaging bone and muscle on pQCT and MRI could enhance comparability across studies and could improve the quality of meta-analyses. PMID:27973379

  15. Onboard utilization of ground control points for image correction. Volume 2: Analysis and simulation results

    NASA Technical Reports Server (NTRS)

    1981-01-01

    An approach to remote sensing that meets future mission requirements was investigated. The deterministic acquisition of data and the rapid correction of data for radiometric effects and image distortions are the most critical limitations of remote sensing. The following topics are discussed: onboard image correction systems, GCP navigation system simulation, GCP analysis, and image correction analysis measurement.

  16. Spreadsheet-Like Image Analysis

    DTIC Science & Technology

    1992-08-01

    1 " DTIC AD-A254 395 S LECTE D, ° AD-E402 350 Technical Report ARPAD-TR-92002 SPREADSHEET-LIKE IMAGE ANALYSIS Paul Willson August 1992 U.S. ARMY...August 1992 4. TITLE AND SUBTITLE 5. FUNDING NUMBERS SPREADSHEET-LIKE IMAGE ANALYSIS 6. AUTHOR(S) Paul Willson 7. PERFORMING ORGANIZATION NAME(S) AND...14. SUBJECT TERMS 15. NUMBER OF PAGES Image analysis , nondestructive inspection, spreadsheet, Macintosh software, 14 neural network, signal processing

  17. Knowledge-Based Image Analysis.

    DTIC Science & Technology

    1981-04-01

    UNCLASSIF1 ED ETL-025s N IIp ETL-0258 AL Ai01319 S"Knowledge-based image analysis u George C. Stockman Barbara A. Lambird I David Lavine Laveen N. Kanal...extraction, verification, region classification, pattern recognition, image analysis . 3 20. A. CT (Continue on rever.. d. It necessary and Identify by...UNCLgSTFTF n In f SECURITY CLASSIFICATION OF THIS PAGE (When Date Entered) .L1 - I Table of Contents Knowledge Based Image Analysis I Preface

  18. Evaluation of Acquisition Strategies for Image-Based Construction Site Monitoring

    NASA Astrophysics Data System (ADS)

    Tuttas, S.; Braun, A.; Borrmann, A.; Stilla, U.

    2016-06-01

    Construction site monitoring is an essential task for keeping track of the ongoing construction work and providing up-to-date information for a Building Information Model (BIM). The BIM contains the as-planned states (geometry, schedule, costs, ...) of a construction project. For updating, the as-built state has to be acquired repeatedly and compared to the as-planned state. In the approach presented here, a 3D representation of the as-built state is calculated from photogrammetric images using multi-view stereo reconstruction. On construction sites one has to cope with several difficulties like security aspects, limited accessibility, occlusions or construction activity. Different acquisition strategies and techniques, namely (i) terrestrial acquisition with a hand-held camera, (ii) aerial acquisition using a Unmanned Aerial Vehicle (UAV) and (iii) acquisition using a fixed stereo camera pair at the boom of the crane, are tested on three test sites. They are assessed considering the special needs for the monitoring tasks and limitations on construction sites. The three scenarios are evaluated based on the ability of automation, the required effort for acquisition, the necessary equipment and its maintaining, disturbance of the construction works, and on the accuracy and completeness of the resulting point clouds. Based on the experiences during the test cases the following conclusions can be drawn: Terrestrial acquisition has the lowest requirements on the device setup but lacks on automation and coverage. The crane camera shows the lowest flexibility but the highest grade of automation. The UAV approach can provide the best coverage by combining nadir and oblique views, but can be limited by obstacles and security aspects. The accuracy of the point clouds is evaluated based on plane fitting of selected building parts. The RMS errors of the fitted parts range from 1 to a few cm for the UAV and the hand-held scenario. First results show that the crane camera

  19. Ultrashort echo time imaging using pointwise encoding time reduction with radial acquisition (PETRA).

    PubMed

    Grodzki, David M; Jakob, Peter M; Heismann, Bjoern

    2012-02-01

    Sequences with ultrashort echo times enable new applications of MRI, including bone, tendon, ligament, and dental imaging. In this article, a sequence is presented that achieves the shortest possible encoding time for each k-space point, limited by pulse length, hardware switching times, and gradient performance of the scanner. In pointwise encoding time reduction with radial acquisition (PETRA), outer k-space is filled with radial half-projections, whereas the centre is measured single pointwise on a Cartesian trajectory. This hybrid sequence combines the features of single point imaging with radial projection imaging. No hardware changes are required. Using this method, 3D images with an isotropic resolution of 1 mm can be obtained in less than 3 minutes. The differences between PETRA and the ultrashort echo time (UTE) sequence are evaluated by simulation and phantom measurements. Advantages of pointwise encoding time reduction with radial acquisition are shown for tissue with a T(2) below 1 ms. The signal to noise ratio and Contrast-to-noise ratio (CNR) performance, as well as possible limitations of the approach, are investigated. In-vivo head, knee, ankle, and wrist examples are presented to prove the feasibility of the sequence. In summary, fast imaging with ultrashort echo time is enabled by PETRA and may help to establish new routine clinical applications of ultrashort echo time sequences.

  20. Point-and-stare operation and high-speed image acquisition in real-time hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Driver, Richard D.; Bannon, David P.; Ciccone, Domenic; Hill, Sam L.

    2010-04-01

    The design and optical performance of a small-footprint, low-power, turnkey, Point-And-Stare hyperspectral analyzer, capable of fully automated field deployment in remote and harsh environments, is described. The unit is packaged for outdoor operation in an IP56 protected air-conditioned enclosure and includes a mechanically ruggedized fully reflective, aberration-corrected hyperspectral VNIR (400-1000 nm) spectrometer with a board-level detector optimized for point and stare operation, an on-board computer capable of full system data-acquisition and control, and a fully functioning internal hyperspectral calibration system for in-situ system spectral calibration and verification. Performance data on the unit under extremes of real-time survey operation and high spatial and high spectral resolution will be discussed. Hyperspectral acquisition including full parameter tracking is achieved by the addition of a fiber-optic based downwelling spectral channel for solar illumination tracking during hyperspectral acquisition and the use of other sensors for spatial and directional tracking to pinpoint view location. The system is mounted on a Pan-And-Tilt device, automatically controlled from the analyzer's on-board computer, making the HyperspecTM particularly adaptable for base security, border protection and remote deployments. A hyperspectral macro library has been developed to control hyperspectral image acquisition, system calibration and scene location control. The software allows the system to be operated in a fully automatic mode or under direct operator control through a GigE interface.

  1. Evolutionary analysis of iron (Fe) acquisition system in Marchantia polymorpha.

    PubMed

    Lo, Jing-Chi; Tsednee, Munkhtsetseg; Lo, Ying-Chu; Yang, Shun-Chung; Hu, Jer-Ming; Ishizaki, Kimitsune; Kohchi, Takayuki; Lee, Der-Chuen; Yeh, Kuo-Chen

    2016-07-01

    To acquire appropriate iron (Fe), vascular plants have developed two unique strategies, the reduction-based strategy I of nongraminaceous plants for Fe(2+) and the chelation-based strategy II of graminaceous plants for Fe(3+) . However, the mechanism of Fe uptake in bryophytes, the earliest diverging branch of land plants and dominant in gametophyte generation is less clear. Fe isotope fractionation analysis demonstrated that the liverwort Marchantia polymorpha uses reduction-based Fe acquisition. Enhanced activities of ferric chelate reductase and proton ATPase were detected under Fe-deficient conditions. However, M. polymorpha did not show mugineic acid family phytosiderophores, the key components of strategy II, or the precursor nicotianamine. Five ZIP (ZRT/IRT-like protein) homologs were identified and speculated to be involved in Fe uptake in M. polymorpha. MpZIP3 knockdown conferred reduced growth under Fe-deficient conditions, and MpZIP3 overexpression increased Fe content under excess Fe. Thus, a nonvascular liverwort, M. polymorpha, uses strategy I for Fe acquisition. This system may have been acquired in the common ancestor of land plants and coopted from the gametophyte to sporophyte generation in the evolution of land plants.

  2. TU-AB-207A-01: Image Acquisition Physics and Hardware.

    PubMed

    Li, B

    2016-06-01

    Practicing medical physicists are often time charged with the tasks of evaluating and troubleshooting complex image quality issues related to CT scanners. This course will equip them with a solid and practical understanding of common CT imaging chain and its major components with emphasis on acquisition physics and hardware, reconstruction, artifacts, image quality, dose, and advanced clinical applications. The core objective is to explain the effects of these major system components on the image quality. This course will not focus on the rapid-changing advanced technologies given the two-hour time limit, but the fundamental principles discussed in this course may facilitate better understanding of those more complicated technologies. The course will begin with an overview of CT acquisition physics and geometry. X-ray tube and CT detector are important acquisition hardware critical to the overall image quality. Each of these two subsystems consists of several major components. An in-depth description of the function and failure modes of these components will be provided. Examples of artifacts related to these failure modes will be presented: off-focal radiation, tube arcing, heel effect, oil bubble, offset drift effect, cross-talk effect, and bad pixels. The fundamentals of CT image reconstruction will first be discussed on an intuitive level. Approaches that do not require rigorous derivation of mathematical formulations will be presented. This is followed by a detailed derivation of the Fourier slice theorem: the foundation of the FBP algorithm. FBP for parallel-beam, fan-beam, and cone-beam geometries will be discussed. To address the issue of radiation dose related to x-ray CT, recent advances in iterative reconstruction, their advantages, and clinical applications will also be described. Because of the nature of fundamental physics and mathematics, limitations in data acquisition, and non-ideal conditions of major system components, image artifact often arise

  3. Computer graphic method for direct correspondence image acquisition used in full parallax holographic stereograms

    NASA Astrophysics Data System (ADS)

    Madrid Sánchez, Alejandro; Velásquez Prieto, Daniel

    2016-09-01

    The holoprinter technology based on holographic stereograms has generated a fast development in holographic display applications by the holographic recording of a 2D image sequence with information of a 3D scene, which could be real or computer generated. The images used in holographic stereograms initially start from the acquisition of the different image perspectives of the 3D scene by the re-centering camera configuration and then, this images must be rearranged before the optical recording. This paper proposes a method to acquire the required images or hogel images in one step without using rearrange algorithms, the method uses a virtual camera that moves along a virtual rail by conventional computer graphics software. The proposed method reduced the time required to obtain the hogel images and enhance the quality of the 3D holographic images; it also can be applied in different computer graphics software. To validate the method, a full parallax holographic stereogram was made for a computer generated object.

  4. A uniform method for analytically modeling mulit-target acquisition with independent networked imaging sensors

    NASA Astrophysics Data System (ADS)

    Friedman, Melvin

    2014-05-01

    The problem solved in this paper is easily stated: for a scenario with 𝑛 networked and moving imaging sensors, 𝑚 moving targets and 𝑘 independent observers searching imagery produced by the 𝑛 moving sensors, analytically model system target acquisition probability for each target as a function of time. Information input into the model is the time dependence of 𝘗∞ and 𝜏, two parameters that describe observer-sensor-atmosphere-range-target properties of the target acquisition system for the case where neither the sensor nor target is moving. The parameter 𝘗∞ can be calculated by the NV-IPM model and 𝜏 is estimated empirically from 𝘗∞. In this model 𝑛, 𝑚 and 𝑘 are integers and 𝑘 can be less than, equal to or greater than 𝑛. Increasing 𝑛 and 𝑘 results in a substantial increase in target acquisition probabilities. Because the sensors are networked, a target is said to be detected the moment the first of the 𝑘 observers declares the target. The model applies to time-limited or time-unlimited search, and applies to any imaging sensors operating in any wavelength band provided each sensor can be described by 𝘗∞ and 𝜏 parameters.

  5. Effects of a patient’s name and image on medical knowledge acquisition

    PubMed Central

    Guajardo, Jesus R.; Petershack, Jean A.; Caplow, Julie A.; Littlefield, John H.

    2015-01-01

    Purpose To assess whether there are differences in medical students’ (MS) knowledge acquisition after being provided a virtual patient (VP) case summary with a patient’s name and facial picture included compared to no patient’s name or image. Method 76 MS from four clerkship blocks participated. Blocks one and three (Treatment group) were provided case materials containing the patient’s name and facial picture while blocks two and four (Control group) were provided similar materials without the patient’s name or image. Knowledge acquisition was evaluated with a multiple-choice-question examination (CQA_K). Results Treatment group CQA_K scores were 64.6% (block one, n = 18) and 76.0% (block three, n = 22). Control group scores were 71.7%, (block two, n = 17) and 68.4% (block four, n = 19). ANOVA F-test among the four block mean scores was not significant; F (3, 72) = 1.68, p = 0.18, η2=0.07. Only 22.2% and 27.3% of the MS from blocks one and three respectively correctly recalled the patient’s name while 16.7% and 40.9% recalled the correct final diagnosis of the patient. Conclusions These results suggest that including a patient’s name and facial picture on reading materials may not improve MS knowledge acquisition. Corroborating studies should be performed before applying these results to the design of instructional materials. PMID:27004072

  6. Design and analysis of Galileo sun acquisition algorithm

    NASA Technical Reports Server (NTRS)

    Lin, H.-S.

    1981-01-01

    The Galileo sun acquisition algorithm is used to align the spacecraft antenna with the sun in order to determine spacecraft attitude. It is also used to estimate the spin rate when the spacecraft antenna is not sun oriented, and is capable of performing a rhumb line turn maneuver in the case of two gyro failures. The design of the algorithm is presented in detail along with software implementation at the flowchart level. The six major portions of the algorithm are considered: initialization, sensor measurement mapping, path selection logic, sun detection logic, termination logic, and burn command generation. Analysis is performed to determine the major parameters of the algorithm, and results are verified by computer simulations.

  7. Retinal oximetry based on nonsimultaneous image acquisition using a conventional fundus camera.

    PubMed

    Kim, Sun Kwon; Kim, Dong Myung; Suh, Min Hee; Kim, Martha; Kim, Hee Chan

    2011-08-01

    To measure the retinal arteriole and venule oxygen saturation (SO(2)) using a conventional fundus camera, retinal oximetry based on nonsimultaneous image acquisition was developed and evaluated. Two retinal images were sequentially acquired using a conventional fundus camera with two bandpass filters (568 nm: isobestic, 600 nm: nonisobestic wavelength), one after another, instead of a built-in green filter. The images were registered to compensate for the differences caused by eye movements during the image acquisition. Retinal SO(2) was measured using two wavelength oximetry. To evaluate sensitivity of the proposed method, SO(2) in the arterioles and venules before and after inhalation of 100% O(2) were compared, respectively, in 11 healthy subjects. After inhalation of 100% O(2), SO(2) increased from 96.0 ±6.0% to 98.8% ±7.1% in the arterioles (p=0.002) and from 54.0 ±8.0% to 66.7% ±7.2% in the venules (p=0.005) (paired t-test, n=11). Reproducibility of the method was 2.6% and 5.2% in the arterioles and venules, respectively (average standard deviation of five measurements, n=11).

  8. Spotlight-8 Image Analysis Software

    NASA Technical Reports Server (NTRS)

    Klimek, Robert; Wright, Ted

    2006-01-01

    Spotlight is a cross-platform GUI-based software package designed to perform image analysis on sequences of images generated by combustion and fluid physics experiments run in a microgravity environment. Spotlight can perform analysis on a single image in an interactive mode or perform analysis on a sequence of images in an automated fashion. Image processing operations can be employed to enhance the image before various statistics and measurement operations are performed. An arbitrarily large number of objects can be analyzed simultaneously with independent areas of interest. Spotlight saves results in a text file that can be imported into other programs for graphing or further analysis. Spotlight can be run on Microsoft Windows, Linux, and Apple OS X platforms.

  9. Lossy cardiac x-ray image compression based on acquisition noise

    NASA Astrophysics Data System (ADS)

    de Bruijn, Frederik J.; Slump, Cornelis H.

    1997-05-01

    In lossy medical image compression, the requirements for the preservation of diagnostic integrity cannot be easily formulated in terms of a perceptual model. Especially since, in reality, human visual perception is dependent on numerous factors such as the viewing conditions and psycho-visual factors. Therefore, we investigate the possibility to develop alternative measures for data loss, based on the characteristics of the acquisition system, in our case, a digital cardiac imaging system. In general, due to the low exposure, cardiac x-ray images tend to be relatively noisy. The main noise contributions are quantum noise and electrical noise. The electrical noise is not correlated with the signal. In addition, the signal can be transformed such that the correlated Poisson-distributed quantum noise is transformed into an additional zero-mean Gaussian noise source which is uncorrelated with the signal. Furthermore, the systems modulation transfer function imposes a known spatial-frequency limitation to the output signal. In the assumption that noise which is not correlated with the signal contains no diagnostic information, we have derived a compression measure based on the acquisition parameters of a digital cardiac imaging system. The measure is used for bit- assignment and quantization of transform coefficients. We present a blockwise-DCT compression algorithm which is based on the conventional JPEG-standard. However, the bit- assignment to the transform coefficients is now determined by an assumed noise variance for each coefficient, for a given set of acquisition parameters. Experiments with the algorithm indicate that a bit rate of 0.6 bit/pixel is feasible, without apparent loss of clinical information.

  10. The impact of cine EPID image acquisition frame rate on markerless soft-tissue tracking

    SciTech Connect

    Yip, Stephen Rottmann, Joerg; Berbeco, Ross

    2014-06-15

    Purpose: Although reduction of the cine electronic portal imaging device (EPID) acquisition frame rate through multiple frame averaging may reduce hardware memory burden and decrease image noise, it can hinder the continuity of soft-tissue motion leading to poor autotracking results. The impact of motion blurring and image noise on the tracking performance was investigated. Methods: Phantom and patient images were acquired at a frame rate of 12.87 Hz with an amorphous silicon portal imager (AS1000, Varian Medical Systems, Palo Alto, CA). The maximum frame rate of 12.87 Hz is imposed by the EPID. Low frame rate images were obtained by continuous frame averaging. A previously validated tracking algorithm was employed for autotracking. The difference between the programmed and autotracked positions of a Las Vegas phantom moving in the superior-inferior direction defined the tracking error (δ). Motion blurring was assessed by measuring the area change of the circle with the greatest depth. Additionally, lung tumors on 1747 frames acquired at 11 field angles from four radiotherapy patients are manually and automatically tracked with varying frame averaging. δ was defined by the position difference of the two tracking methods. Image noise was defined as the standard deviation of the background intensity. Motion blurring and image noise are correlated with δ using Pearson correlation coefficient (R). Results: For both phantom and patient studies, the autotracking errors increased at frame rates lower than 4.29 Hz. Above 4.29 Hz, changes in errors were negligible withδ < 1.60 mm. Motion blurring and image noise were observed to increase and decrease with frame averaging, respectively. Motion blurring and tracking errors were significantly correlated for the phantom (R = 0.94) and patient studies (R = 0.72). Moderate to poor correlation was found between image noise and tracking error with R −0.58 and −0.19 for both studies, respectively. Conclusions: Cine EPID

  11. Oncological image analysis: medical and molecular image analysis

    NASA Astrophysics Data System (ADS)

    Brady, Michael

    2007-03-01

    This paper summarises the work we have been doing on joint projects with GE Healthcare on colorectal and liver cancer, and with Siemens Molecular Imaging on dynamic PET. First, we recall the salient facts about cancer and oncological image analysis. Then we introduce some of the work that we have done on analysing clinical MRI images of colorectal and liver cancer, specifically the detection of lymph nodes and segmentation of the circumferential resection margin. In the second part of the paper, we shift attention to the complementary aspect of molecular image analysis, illustrating our approach with some recent work on: tumour acidosis, tumour hypoxia, and multiply drug resistant tumours.

  12. QR-on-a-chip: a computer-recognizable micro-pattern engraved microfluidic device for high-throughput image acquisition.

    PubMed

    Yun, Kyungwon; Lee, Hyunjae; Bang, Hyunwoo; Jeon, Noo Li

    2016-02-21

    This study proposes a novel way to achieve high-throughput image acquisition based on a computer-recognizable micro-pattern implemented on a microfluidic device. We integrated the QR code, a two-dimensional barcode system, onto the microfluidic device to simplify imaging of multiple ROIs (regions of interest). A standard QR code pattern was modified to arrays of cylindrical structures of polydimethylsiloxane (PDMS). Utilizing the recognition of the micro-pattern, the proposed system enables: (1) device identification, which allows referencing additional information of the device, such as device imaging sequences or the ROIs and (2) composing a coordinate system for an arbitrarily located microfluidic device with respect to the stage. Based on these functionalities, the proposed method performs one-step high-throughput imaging for data acquisition in microfluidic devices without further manual exploration and locating of the desired ROIs. In our experience, the proposed method significantly reduced the time for the preparation of an acquisition. We expect that the method will innovatively improve the prototype device data acquisition and analysis.

  13. Task-driven image acquisition and reconstruction in cone-beam CT.

    PubMed

    Gang, Grace J; Stayman, J Webster; Ehtiati, Tina; Siewerdsen, Jeffrey H

    2015-04-21

    This work introduces a task-driven imaging framework that incorporates a mathematical definition of the imaging task, a model of the imaging system, and a patient-specific anatomical model to prospectively design image acquisition and reconstruction techniques to optimize task performance. The framework is applied to joint optimization of tube current modulation, view-dependent reconstruction kernel, and orbital tilt in cone-beam CT. The system model considers a cone-beam CT system incorporating a flat-panel detector and 3D filtered backprojection and accurately describes the spatially varying noise and resolution over a wide range of imaging parameters in the presence of a realistic anatomical model. Task-based detectability index (d') is incorporated as the objective function in a task-driven optimization of image acquisition and reconstruction techniques. The orbital tilt was optimized through an exhaustive search across tilt angles ranging ± 30°. For each tilt angle, the view-dependent tube current and reconstruction kernel (i.e. the modulation profiles) that maximized detectability were identified via an alternating optimization. The task-driven approach was compared with conventional unmodulated and automatic exposure control (AEC) strategies for a variety of imaging tasks and anthropomorphic phantoms. The task-driven strategy outperformed the unmodulated and AEC cases for all tasks. For example, d' for a sphere detection task in a head phantom was improved by 30% compared to the unmodulated case by using smoother kernels for noisy views and distributing mAs across less noisy views (at fixed total mAs) in a manner that was beneficial to task performance. Similarly for detection of a line-pair pattern, the task-driven approach increased d' by 80% compared to no modulation by means of view-dependent mA and kernel selection that yields modulation transfer function and noise-power spectrum optimal to the task. Optimization of orbital tilt identified the tilt

  14. Task-driven image acquisition and reconstruction in cone-beam CT

    PubMed Central

    Gang, Grace J.; Stayman, J. Webster; Ehtiati, Tina; Siewerdsen, Jeffrey H.

    2015-01-01

    This work introduces a task-driven imaging framework that incorporates a mathematical definition of the imaging task, a model of the imaging system, and a patient-specific anatomical model to prospectively design image acquisition and reconstruction techniques to optimize task performance. The framework is applied to joint optimization of tube current modulation, view-dependent reconstruction kernel, and orbital tilt in cone-beam CT. The system model considers a cone-beam CT system incorporating a flat-panel detector and 3D filtered backprojection and accurately describes the spatially varying noise and resolution over a wide range of imaging parameters and in the presence of a realistic anatomical model. Task-based detectability index (d') is incorporated as the objective function in a task-driven optimization of image acquisition and reconstruction techniques. The orbital tilt was optimized through an exhaustive search across tilt angles ranging ±30°. For each tilt angle, the view-dependent tube current and reconstruction kernel (i.e., the modulation profiles) that maximized detectability were identified via an alternating optimization. The task-driven approach was compared with conventional unmodulated and automatic exposure control (AEC) strategies for a variety of imaging tasks and anthropomorphic phantoms. The task-driven strategy outperformed the unmodulated and AEC cases for all tasks. For example, d' for a sphere detection task in a head phantom was improved by 30% compared to the unmodulated case by using smoother kernels for noisy views and distributing mAs across less noisy views (at fixed total mAs) in a manner that was beneficial to task performance. Similarly for detection of a line-pair pattern, the task-driven approach increased d' by 80% compared to no modulation by means of view-dependent mA and kernel selection that yields modulation transfer function and noise-power spectrum optimal to the task. Optimization of orbital tilt identified the

  15. Task-driven image acquisition and reconstruction in cone-beam CT

    NASA Astrophysics Data System (ADS)

    Gang, Grace J.; Webster Stayman, J.; Ehtiati, Tina; Siewerdsen, Jeffrey H.

    2015-04-01

    This work introduces a task-driven imaging framework that incorporates a mathematical definition of the imaging task, a model of the imaging system, and a patient-specific anatomical model to prospectively design image acquisition and reconstruction techniques to optimize task performance. The framework is applied to joint optimization of tube current modulation, view-dependent reconstruction kernel, and orbital tilt in cone-beam CT. The system model considers a cone-beam CT system incorporating a flat-panel detector and 3D filtered backprojection and accurately describes the spatially varying noise and resolution over a wide range of imaging parameters in the presence of a realistic anatomical model. Task-based detectability index (d‧) is incorporated as the objective function in a task-driven optimization of image acquisition and reconstruction techniques. The orbital tilt was optimized through an exhaustive search across tilt angles ranging ±30°. For each tilt angle, the view-dependent tube current and reconstruction kernel (i.e. the modulation profiles) that maximized detectability were identified via an alternating optimization. The task-driven approach was compared with conventional unmodulated and automatic exposure control (AEC) strategies for a variety of imaging tasks and anthropomorphic phantoms. The task-driven strategy outperformed the unmodulated and AEC cases for all tasks. For example, d‧ for a sphere detection task in a head phantom was improved by 30% compared to the unmodulated case by using smoother kernels for noisy views and distributing mAs across less noisy views (at fixed total mAs) in a manner that was beneficial to task performance. Similarly for detection of a line-pair pattern, the task-driven approach increased d‧ by 80% compared to no modulation by means of view-dependent mA and kernel selection that yields modulation transfer function and noise-power spectrum optimal to the task. Optimization of orbital tilt identified the

  16. Development of a 3-D data acquisition system for human facial imaging

    NASA Astrophysics Data System (ADS)

    Marshall, Stephen J.; Rixon, R. C.; Whiteford, Don N.; Wells, Peter J.; Powell, S. J.

    1990-07-01

    While preparing to conduct human facial surgery, it is necessary to visualise the effects of proposed surgery on the patient's appearance. This visualisation is of great benefit to both surgeon and patient, and has traditionally been achieved by the manual manipulation of photographs. Technological developments in the areas of computer-aided design and optical sensing now make it possible to construct a computer-based imaging system which can simulate the effects of facial surgery on patients. A collaborative project with the aim of constructing a prototype facial imaging system is under way between the National Engineering Laboratory and St George's Hospital. The proposed system will acquire, display and manipulate 3-dimensional facial images of patients requiring facial surgery. The feasibility of using two NEL developed optical measurement methods for 3-D facial data acquisition had been established by their successful application to the measurement of dummy heads. The two optical measurement systems, the NEL Auto-MATE moire fringe contouring system and the NEL STRIPE laser scanning triangulation system, were further developed to adapt them for use in facial imaging and additional tests carried out in which emphasis was placed on the use of live human subjects. The knowledge gained in the execution of the tests enabled the selection of the most suitable of the two methods studied for facial data acquisition. A full description of the methods and equipment used in the study will be given. Additionally, work on the effects of the quality and quantity of measurement data on the facial image will be described. Finally, the question of how best to provide display and manipulation of the facial images will be addressed.

  17. Investigations on the efficiency of cardiac-gated methods for the acquisition of diffusion-weighted images

    NASA Astrophysics Data System (ADS)

    Nunes, Rita G.; Jezzard, Peter; Clare, Stuart

    2005-11-01

    Diffusion-weighted images are inherently very sensitive to motion. Pulsatile motion of the brain can give rise to artifactual signal attenuation leading to over-estimation of the apparent diffusion coefficients, even with snapshot echo planar imaging. Such miscalculations can result in erroneous estimates of the principal diffusion directions. Cardiac gating can be performed to confine acquisition to the quiet portion of the cycle. Although effective, this approach leads to significantly longer acquisition times. On the other hand, it has been demonstrated that pulsatile motion is not significant in regions above the corpus callosum. To reduce acquisition times and improve the efficiency of whole brain cardiac-gated acquisitions, the upper slices of the brain can be imaged during systole, reserving diastole for those slices most affected by pulsatile motion. The merits and disadvantages of this optimized approach are investigated here, in comparison to a more standard gating method and to the non-gated approach.

  18. Tomographic spectral imaging: analysis of localized corrosion.

    SciTech Connect

    Michael, Joseph Richard; Kotula, Paul Gabriel; Keenan, Michael Robert

    2005-02-01

    Microanalysis is typically performed to analyze the near surface of materials. There are many instances where chemical information about the third spatial dimension is essential to the solution of materials analyses. The majority of 3D analyses however focus on limited spectral acquisition and/or analysis. For truly comprehensive 3D chemical characterization, 4D spectral images (a complete spectrum from each volume element of a region of a specimen) are needed. Furthermore, a robust statistical method is needed to extract the maximum amount of chemical information from that extremely large amount of data. In this paper, an example of the acquisition and multivariate statistical analysis of 4D (3-spatial and 1-spectral dimension) x-ray spectral images is described. The method of utilizing a single- or dual-beam FIB (w/o or w/SEM) to get at 3D chemistry has been described by others with respect to secondary-ion mass spectrometry. The basic methodology described in those works has been modified for comprehensive x-ray microanalysis in a dual-beam FIB/SEM (FEI Co. DB-235). In brief, the FIB is used to serially section a site-specific region of a sample and then the electron beam is rastered over the exposed surfaces with x-ray spectral images being acquired at each section. All this is performed without rotating or tilting the specimen between FIB cutting and SEM imaging/x-ray spectral image acquisition. The resultant 4D spectral image is then unfolded (number of volume elements by number of channels) and subjected to the same multivariate curve resolution (MCR) approach that has proven successful for the analysis of lower-dimension x-ray spectral images. The TSI data sets can be in excess of 4Gbytes. This problem has been overcome (for now) and images up to 6Gbytes have been analyzed in this work. The method for analyzing such large spectral images will be described in this presentation. A comprehensive 3D chemical analysis was performed on several corrosion specimens

  19. Spectral analysis for automated exploration and sample acquisition

    NASA Astrophysics Data System (ADS)

    Eberlein, Susan; Yates, Gigi

    1992-05-01

    Future space exploration missions will rely heavily on the use of complex instrument data for determining the geologic, chemical, and elemental character of planetary surfaces. One important instrument is the imaging spectrometer, which collects complete images in multiple discrete wavelengths in the visible and infrared regions of the spectrum. Extensive computational effort is required to extract information from such high-dimensional data. A hierarchical classification scheme allows multispectral data to be analyzed for purposes of mineral classification while limiting the overall computational requirements. The hierarchical classifier exploits the tunability of a new type of imaging spectrometer which is based on an acousto-optic tunable filter. This spectrometer collects a complete image in each wavelength passband without spatial scanning. It may be programmed to scan through a range of wavelengths or to collect only specific bands for data analysis. Spectral classification activities employ artificial neural networks, trained to recognize a number of mineral classes. Analysis of the trained networks has proven useful in determining which subsets of spectral bands should be employed at each step of the hierarchical classifier. The network classifiers are capable of recognizing all mineral types which were included in the training set. In addition, the major components of many mineral mixtures can also be recognized. This capability may prove useful for a system designed to evaluate data in a strange environment where details of the mineral composition are not known in advance.

  20. Spectral analysis for automated exploration and sample acquisition

    NASA Technical Reports Server (NTRS)

    Eberlein, Susan; Yates, Gigi

    1992-01-01

    Future space exploration missions will rely heavily on the use of complex instrument data for determining the geologic, chemical, and elemental character of planetary surfaces. One important instrument is the imaging spectrometer, which collects complete images in multiple discrete wavelengths in the visible and infrared regions of the spectrum. Extensive computational effort is required to extract information from such high-dimensional data. A hierarchical classification scheme allows multispectral data to be analyzed for purposes of mineral classification while limiting the overall computational requirements. The hierarchical classifier exploits the tunability of a new type of imaging spectrometer which is based on an acousto-optic tunable filter. This spectrometer collects a complete image in each wavelength passband without spatial scanning. It may be programmed to scan through a range of wavelengths or to collect only specific bands for data analysis. Spectral classification activities employ artificial neural networks, trained to recognize a number of mineral classes. Analysis of the trained networks has proven useful in determining which subsets of spectral bands should be employed at each step of the hierarchical classifier. The network classifiers are capable of recognizing all mineral types which were included in the training set. In addition, the major components of many mineral mixtures can also be recognized. This capability may prove useful for a system designed to evaluate data in a strange environment where details of the mineral composition are not known in advance.

  1. Variability of textural features in FDG PET images due to different acquisition modes and reconstruction parameters

    PubMed Central

    GALAVIS, PAULINA E.; HOLLENSEN, CHRISTIAN; JALLOW, NGONEH; PALIWAL, BHUDATT; JERAJ, ROBERT

    2014-01-01

    Background Characterization of textural features (spatial distributions of image intensity levels) has been considered as a tool for automatic tumor segmentation. The purpose of this work is to study the variability of the textural features in PET images due to different acquisition modes and reconstruction parameters. Material and methods Twenty patients with solid tumors underwent PET/CT scans on a GE Discovery VCT scanner, 45–60 minutes post-injection of 10 mCi of [18F]FDG. Scans were acquired in both 2D and 3D modes. For each acquisition the raw PET data was reconstructed using five different reconstruction parameters. Lesions were segmented on a default image using the threshold of 40% of maximum SUV. Fifty different texture features were calculated inside the tumors. The range of variations of the features were calculated with respect to the average value. Results Fifty textural features were classified based on the range of variation in three categories: small, intermediate and large variability. Features with small variability (range ≤ 5%) were entropy-first order, energy, maximal correlation coefficient (second order feature) and low-gray level run emphasis (high-order feature). The features with intermediate variability (10% ≤ range ≤ 25%) were entropy-GLCM, sum entropy, high gray level run emphsis, gray level non-uniformity, small number emphasis, and entropy-NGL. Forty remaining features presented large variations (range > 30%). Conclusion Textural features such as entropy-first order, energy, maximal correlation coefficient, and low-gray level run emphasis exhibited small variations due to different acquisition modes and reconstruction parameters. Features with low level of variations are better candidates for reproducible tumor segmentation. Even though features such as contrast-NGTD, coarseness, homogeneity, and busyness have been previously used, our data indicated that these features presented large variations, therefore they could not be

  2. Wide-field flexible endoscope for simultaneous color and NIR fluorescence image acquisition during surveillance colonoscopy

    NASA Astrophysics Data System (ADS)

    García-Allende, P. Beatriz; Nagengast, Wouter B.; Glatz, Jürgen; Ntziachristos, Vasilis

    2013-03-01

    Colorectal cancer (CRC) is the third most common form of cancer and, despite recent declines in both incidence and mortality, it still remains the second leading cause of cancer-related deaths in the western world. Colonoscopy is the standard for detection and removal of premalignant lesions to prevent CRC. The major challenges that physicians face during surveillance colonoscopy are the high adenoma miss-rates and the lack of functional information to facilitate decision-making concerning which lesions to remove. Targeted imaging with NIR fluorescence would address these limitations. Tissue penetration is increased in the NIR range while the combination with targeted NIR fluorescent agents provides molecularly specific detection of cancer cells, i.e. a red-flag detection strategy that allows tumor imaging with optimal sensitivity and specificity. The development of a flexible endoscopic fluorescence imaging method that can be integrated with standard medical endoscopes and facilitates the clinical use of this potential is described in this work. A semi-disposable coherent fiber optic imaging bundle that is traditionally employed in the exploration of biliary and pancreatic ducts is proposed, since it is long and thin enough to be guided through the working channel of a traditional video colonoscope allowing visualization of proximal lesions in the colon. A custom developed zoom system magnifies the image of the proximal end of the imaging bundle to fill the dimensions of two cameras operating in parallel providing the simultaneous color and fluorescence video acquisition.

  3. Four-channel surface coil array for sequential CW-EPR image acquisition

    NASA Astrophysics Data System (ADS)

    Enomoto, Ayano; Emoto, Miho; Fujii, Hirotada; Hirata, Hiroshi

    2013-09-01

    This article describes a four-channel surface coil array to increase the area of visualization for continuous-wave electron paramagnetic resonance (CW-EPR) imaging. A 776-MHz surface coil array was constructed with four independent surface coil resonators and three kinds of switches. Control circuits for switching the resonators were also built to sequentially perform EPR image acquisition for each resonator. The resonance frequencies of the resonators were shifted using PIN diode switches to decouple the inductively coupled coils. To investigate the area of visualization with the surface coil array, three-dimensional EPR imaging was performed using a glass cell phantom filled with a solution of nitroxyl radicals. The area of visualization obtained with the surface coil array was increased approximately 3.5-fold in comparison to that with a single surface coil resonator. Furthermore, to demonstrate the applicability of this surface coil array to animal imaging, three-dimensional EPR imaging was performed in a living mouse with an exogenously injected nitroxyl radical imaging agent.

  4. TU-D-BRB-01: Dual-Energy CT: Techniques in Acquisition and Image Processing.

    PubMed

    Pelc, N

    2016-06-01

    Dual-energy CT technology is becoming increasingly available to the medical imaging community. In addition, several models of CT simulators sold for use in radiation therapy departments now feature dual-energy technology. The images provided by dual-energy CT scanners add new information to the radiation treatment planning process; multiple spectral components can be used to separate and identify material composition as well as generate virtual monoenergetic images. In turn, this information could be used to investigate pathologic processes, separate the properties of contrast agents from soft tissues, assess tissue response to therapy, and other applications of therapeutic interest. Additionally, the decomposition of materials in images could directly integrate with and impact the accuracy of dose calculation algorithms. This symposium will explore methods of generating dual-energy CT images, spectral and image analysis algorithms, current and future applications of interest in oncologic imaging, and unique considerations when using dualenergy CT images in the radiation treatment planning process.

  5. Histopathological Image Analysis: A Review

    PubMed Central

    Gurcan, Metin N.; Boucheron, Laura; Can, Ali; Madabhushi, Anant; Rajpoot, Nasir; Yener, Bulent

    2010-01-01

    Over the past decade, dramatic increases in computational power and improvement in image analysis algorithms have allowed the development of powerful computer-assisted analytical approaches to radiological data. With the recent advent of whole slide digital scanners, tissue histopathology slides can now be digitized and stored in digital image form. Consequently, digitized tissue histopathology has now become amenable to the application of computerized image analysis and machine learning techniques. Analogous to the role of computer-assisted diagnosis (CAD) algorithms in medical imaging to complement the opinion of a radiologist, CAD algorithms have begun to be developed for disease detection, diagnosis, and prognosis prediction to complement to the opinion of the pathologist. In this paper, we review the recent state of the art CAD technology for digitized histopathology. This paper also briefly describes the development and application of novel image analysis technology for a few specific histopathology related problems being pursued in the United States and Europe. PMID:20671804

  6. Cardiovascular Magnetic Resonance in Cardiology Practice: A Concise Guide to Image Acquisition and Clinical Interpretation.

    PubMed

    Valbuena-López, Silvia; Hinojar, Rocío; Puntmann, Valentina O

    2016-02-01

    Cardiovascular magnetic resonance plays an increasingly important role in routine cardiology clinical practice. It is a versatile imaging modality that allows highly accurate, broad and in-depth assessment of cardiac function and structure and provides information on pertinent clinical questions in diseases such as ischemic heart disease, nonischemic cardiomyopathies, and heart failure, as well as allowing unique indications, such as the assessment and quantification of myocardial iron overload or infiltration. Increasing evidence for the role of cardiovascular magnetic resonance, together with the spread of knowledge and skill outside expert centers, has afforded greater access for patients and wider clinical experience. This review provides a snapshot of cardiovascular magnetic resonance in modern clinical practice by linking image acquisition and postprocessing with effective delivery of the clinical meaning.

  7. Optimizing Uas Image Acquisition and Geo-Registration for Precision Agriculture

    NASA Astrophysics Data System (ADS)

    Hearst, A. A.; Cherkauer, K. A.; Rainey, K. M.

    2014-12-01

    Unmanned Aircraft Systems (UASs) can acquire imagery of crop fields in various spectral bands, including the visible, near-infrared, and thermal portions of the spectrum. By combining techniques of computer vision, photogrammetry, and remote sensing, these images can be stitched into precise, geo-registered maps, which may have applications in precision agriculture and other industries. However, the utility of these maps will depend on their positional accuracy. Therefore, it is important to quantify positional accuracy and consider the tradeoffs between accuracy, field site setup, and the computational requirements for data processing and analysis. This will enable planning of data acquisition and processing to obtain the required accuracy for a given project. This study focuses on developing and evaluating methods for geo-registration of raw aerial frame photos acquired by a small fixed-wing UAS. This includes visual, multispectral, and thermal imagery at 3, 6, and 14 cm/pix resolutions, respectively. The study area is 10 hectares of soybean fields at the Agronomy Center for Research and Education (ACRE) at Purdue University. The dataset consists of imagery from 6 separate days of flights (surveys) and supporting ground measurements. The Direct Sensor Orientation (DiSO) and Integrated Sensor Orientation (InSO) methods for geo-registration are tested using 16 Ground Control Points (GCPs). Subsets of these GCPs are used to test for the effects of different numbers and spatial configurations of GCPs on positional accuracy. The horizontal and vertical Root Mean Squared Error (RMSE) is used as the primary metric of positional accuracy. Preliminary results from 1 of the 6 surveys show that the DiSO method (0 GCPs used) achieved an RMSE in the X, Y, and Z direction of 2.46 m, 1.04 m, and 1.91 m, respectively. InSO using 5 GCPs achieved an RMSE of 0.17 m, 0.13 m, and 0.44 m. InSO using 10 GCPs achieved an RMSE of 0.10 m, 0.09 m, and 0.12 m. Further analysis will identify

  8. Design of multi-mode compatible image acquisition system for HD area array CCD

    NASA Astrophysics Data System (ADS)

    Wang, Chen; Sui, Xiubao

    2014-11-01

    Combining with the current development trend in video surveillance-digitization and high-definition, a multimode-compatible image acquisition system for HD area array CCD is designed. The hardware and software designs of the color video capture system of HD area array CCD KAI-02150 presented by Truesense Imaging company are analyzed, and the structure parameters of the HD area array CCD and the color video gathering principle of the acquisition system are introduced. Then, the CCD control sequence and the timing logic of the whole capture system are realized. The noises of the video signal (KTC noise and 1/f noise) are filtered by using the Correlated Double Sampling (CDS) technique to enhance the signal-to-noise ratio of the system. The compatible designs in both software and hardware for the two other image sensors of the same series: KAI-04050 and KAI-08050 are put forward; the effective pixels of these two HD image sensors are respectively as many as four million and eight million. A Field Programmable Gate Array (FPGA) is adopted as the key controller of the system to perform the modularization design from top to bottom, which realizes the hardware design by software and improves development efficiency. At last, the required time sequence driving is simulated accurately by the use of development platform of Quartus II 12.1 combining with VHDL. The result of the simulation indicates that the driving circuit is characterized by simple framework, low power consumption, and strong anti-interference ability, which meet the demand of miniaturization and high-definition for the current tendency.

  9. A Spartan 6 FPGA-based data acquisition system for dedicated imagers in nuclear medicine

    NASA Astrophysics Data System (ADS)

    Fysikopoulos, E.; Loudos, G.; Georgiou, M.; David, S.; Matsopoulos, G.

    2012-12-01

    We present the development of a four-channel low-cost hardware system for data acquisition, with application in dedicated nuclear medicine imagers. A 12 bit octal channel high-speed analogue to digital converter, with up to 65 Msps sampling rate, was used for the digitization of analogue signals. The digitized data are fed into a field programmable gate array (FPGA), which contains an interface to a bank of double data rate 2 (DDR2)-type memory. The FPGA processes the digitized data and stores the results into the DDR2. An ethernet link was used for data transmission to a personal computer. The embedded system was designed using Xilinx's embedded development kit (EDK) and was based on Xilinx's Microblaze soft-core processor. The system has been evaluated using two different discrete optical detector arrays (a position-sensitive photomultiplier tube and a silicon photomultiplier) with two different pixelated scintillator arrays (BGO, LSO:Ce). The energy resolution for both detectors was approximately 25%. A clear identification of all crystal elements was achieved in all cases. The data rate of the system with this implementation can reach 60 Mbits s-1. The results have shown that this FPGA data acquisition system is a compact and flexible solution for single-photon-detection applications. This paper was originally submitted for inclusion in the special feature on Imaging Systems and Techniques 2011.

  10. Flightspeed Integral Image Analysis Toolkit

    NASA Technical Reports Server (NTRS)

    Thompson, David R.

    2009-01-01

    The Flightspeed Integral Image Analysis Toolkit (FIIAT) is a C library that provides image analysis functions in a single, portable package. It provides basic low-level filtering, texture analysis, and subwindow descriptor for applications dealing with image interpretation and object recognition. Designed with spaceflight in mind, it addresses: Ease of integration (minimal external dependencies) Fast, real-time operation using integer arithmetic where possible (useful for platforms lacking a dedicated floatingpoint processor) Written entirely in C (easily modified) Mostly static memory allocation 8-bit image data The basic goal of the FIIAT library is to compute meaningful numerical descriptors for images or rectangular image regions. These n-vectors can then be used directly for novelty detection or pattern recognition, or as a feature space for higher-level pattern recognition tasks. The library provides routines for leveraging training data to derive descriptors that are most useful for a specific data set. Its runtime algorithms exploit a structure known as the "integral image." This is a caching method that permits fast summation of values within rectangular regions of an image. This integral frame facilitates a wide range of fast image-processing functions. This toolkit has applicability to a wide range of autonomous image analysis tasks in the space-flight domain, including novelty detection, object and scene classification, target detection for autonomous instrument placement, and science analysis of geomorphology. It makes real-time texture and pattern recognition possible for platforms with severe computational restraints. The software provides an order of magnitude speed increase over alternative software libraries currently in use by the research community. FIIAT can commercially support intelligent video cameras used in intelligent surveillance. It is also useful for object recognition by robots or other autonomous vehicles

  11. Optimizing Federal Fleet Vehicle Acquisitions: An Eleven-Agency FY 2012 Analysis

    SciTech Connect

    Singer, M.; Daley, R.

    2015-02-01

    This report focuses on the National Renewable Energy Laboratory's (NREL) fiscal year (FY) 2012 effort that used the NREL Optimal Vehicle Acquisition (NOVA) analysis to identify optimal vehicle acquisition recommendations for eleven diverse federal agencies. Results of the study show that by following a vehicle acquisition plan that maximizes the reduction in greenhouse gas (GHG) emissions, significant progress is also made toward the mandated complementary goals of acquiring alternative fuel vehicles, petroleum use reduction, and alternative fuel use increase.

  12. A novel CT acquisition and analysis technique for breathing motion modeling

    PubMed Central

    Low, Daniel A.; White, Benjamin M.; Lee, Percy P.; Thomas, David H.; Gaudio, Sergio; Jani, Shyam S.; Wu, Xiao; Lamb, James M.

    2013-01-01

    Purpose To report on a novel technique for providing artifact-free quantitative 4DCT image datasets for breathing motion modeling. Methods Commercial clinical four-dimensional computed tomography (4DCT) methods have trouble managing irregular breathing. The resulting images contain motion-induced artifacts that can distort structures and inaccurately characterize breathing motion. We have developed a novel scanning and analysis method for motion-correlated CT that utilizes standard repeated fast helical acquisitions, a simultaneous breathing surrogate measurement, deformable image registration, and a published breathing motion model. Results The motion model differs from the CT-measured motion by an average of 0.72 mm, indicating the precision of the motion model. The integral of the divergence of one of the motion model parameters is predicted to be a constant 1.11 and is found in this case to be 1.09, indicating the accuracy of the motion model. Conclusions The proposed technique shows promise for providing motion-artifact free images at user-selected breathing phases, accurate Hounsfield units, and noise characteristics similar to non-4D CT techniques, at a patient dose similar to or less than current 4DCT techniques. PMID:23640212

  13. Digital image processing and analysis for activated sludge wastewater treatment.

    PubMed

    Khan, Muhammad Burhan; Lee, Xue Yong; Nisar, Humaira; Ng, Choon Aun; Yeap, Kim Ho; Malik, Aamir Saeed

    2015-01-01

    Activated sludge system is generally used in wastewater treatment plants for processing domestic influent. Conventionally the activated sludge wastewater treatment is monitored by measuring physico-chemical parameters like total suspended solids (TSSol), sludge volume index (SVI) and chemical oxygen demand (COD) etc. For the measurement, tests are conducted in the laboratory, which take many hours to give the final measurement. Digital image processing and analysis offers a better alternative not only to monitor and characterize the current state of activated sludge but also to predict the future state. The characterization by image processing and analysis is done by correlating the time evolution of parameters extracted by image analysis of floc and filaments with the physico-chemical parameters. This chapter briefly reviews the activated sludge wastewater treatment; and, procedures of image acquisition, preprocessing, segmentation and analysis in the specific context of activated sludge wastewater treatment. In the latter part additional procedures like z-stacking, image stitching are introduced for wastewater image preprocessing, which are not previously used in the context of activated sludge. Different preprocessing and segmentation techniques are proposed, along with the survey of imaging procedures reported in the literature. Finally the image analysis based morphological parameters and correlation of the parameters with regard to monitoring and prediction of activated sludge are discussed. Hence it is observed that image analysis can play a very useful role in the monitoring of activated sludge wastewater treatment plants.

  14. Image analysis for DNA sequencing

    NASA Astrophysics Data System (ADS)

    Palaniappan, Kannappan; Huang, Thomas S.

    1991-07-01

    There is a great deal of interest in automating the process of DNA (deoxyribonucleic acid) sequencing to support the analysis of genomic DNA such as the Human and Mouse Genome projects. In one class of gel-based sequencing protocols autoradiograph images are generated in the final step and usually require manual interpretation to reconstruct the DNA sequence represented by the image. The need to handle a large volume of sequence information necessitates automation of the manual autoradiograph reading step through image analysis in order to reduce the length of time required to obtain sequence data and reduce transcription errors. Various adaptive image enhancement, segmentation and alignment methods were applied to autoradiograph images. The methods are adaptive to the local characteristics of the image such as noise, background signal, or presence of edges. Once the two-dimensional data is converted to a set of aligned one-dimensional profiles waveform analysis is used to determine the location of each band which represents one nucleotide in the sequence. Different classification strategies including a rule-based approach are investigated to map the profile signals, augmented with the original two-dimensional image data as necessary, to textual DNA sequence information.

  15. A collaborative biomedical image mining framework: application on the image analysis of microscopic kidney biopsies.

    PubMed

    Goudas, T; Doukas, C; Chatziioannou, A; Maglogiannis, I

    2013-01-01

    The analysis and characterization of biomedical image data is a complex procedure involving several processing phases, like data acquisition, preprocessing, segmentation, feature extraction and classification. The proper combination and parameterization of the utilized methods are heavily relying on the given image data set and experiment type. They may thus necessitate advanced image processing and classification knowledge and skills from the side of the biomedical expert. In this work, an application, exploiting web services and applying ontological modeling, is presented, to enable the intelligent creation of image mining workflows. The described tool can be directly integrated to the RapidMiner, Taverna or similar workflow management platforms. A case study dealing with the creation of a sample workflow for the analysis of kidney biopsy microscopy images is presented to demonstrate the functionality of the proposed framework.

  16. Graph-based retrospective 4D image construction from free-breathing MRI slice acquisitions

    NASA Astrophysics Data System (ADS)

    Tong, Yubing; Udupa, Jayaram K.; Ciesielski, Krzysztof C.; McDonough, Joseph M.; Mong, Andrew; Campbell, Robert M.

    2014-03-01

    4D or dynamic imaging of the thorax has many potential applications [1, 2]. CT and MRI offer sufficient speed to acquire motion information via 4D imaging. However they have different constraints and requirements. For both modalities both prospective and retrospective respiratory gating and tracking techniques have been developed [3, 4]. For pediatric imaging, x-ray radiation becomes a primary concern and MRI remains as the de facto choice. The pediatric subjects we deal with often suffer from extreme malformations of their chest wall, diaphragm, and/or spine, as such patient cooperation needed by some of the gating and tracking techniques are difficult to realize without causing patient discomfort. Moreover, we are interested in the mechanical function of their thorax in its natural form in tidal breathing. Therefore free-breathing MRI acquisition is the ideal modality of imaging for these patients. In our set up, for each coronal (or sagittal) slice position, slice images are acquired at a rate of about 200-300 ms/slice over several natural breathing cycles. This produces typically several thousands of slices which contain both the anatomic and dynamic information. However, it is not trivial to form a consistent and well defined 4D volume from these data. In this paper, we present a novel graph-based combinatorial optimization solution for constructing the best possible 4D scene from such data entirely in the digital domain. Our proposed method is purely image-based and does not need breath holding or any external surrogates or instruments to record respiratory motion or tidal volume. Both adult and children patients' data are used to illustrate the performance of the proposed method. Experimental results show that the reconstructed 4D scenes are smooth and consistent spatially and temporally, agreeing with known shape and motion of the lungs.

  17. Errors from Image Analysis

    SciTech Connect

    Wood, William Monford

    2015-02-23

    Presenting a systematic study of the standard analysis of rod-pinch radiographs for obtaining quantitative measurements of areal mass densities, and making suggestions for improving the methodology of obtaining quantitative information from radiographed objects.

  18. APPLICATION OF PRINCIPAL COMPONENT ANALYSIS AND BAYESIAN DECOMPOSITION TO RELAXOGRAPHIC IMAGING

    SciTech Connect

    OCHS,M.F.; STOYANOVA,R.S.; BROWN,T.R.; ROONEY,W.D.; LI,X.; LEE,J.H.; SPRINGER,C.S.

    1999-05-22

    Recent developments in high field imaging have made possible the acquisition of high quality, low noise relaxographic data in reasonable imaging times. The datasets comprise a huge amount of information (>>1 million points) which makes rigorous analysis daunting. Here, the authors present results demonstrating that Principal Component Analysis (PCA) and Bayesian Decomposition (BD) provide powerful methods for relaxographic analysis of T{sub 1} recovery curves and editing of tissue type in resulting images.

  19. Multi-Source Image Analysis.

    DTIC Science & Technology

    1979-12-01

    three sensor systems, but at some test sites only one or two types were utilized. Sensor characteristics were evaluated in relationship to the targets...Multi-source image analysis is an evaluation of remote sensor imagery for military geographic information. The imagery is confined to radar, thermal...heating affect a TIR scanner’s recorded temperature, careful image evaluation can be used to extract valuable military geographic information

  20. Optimized acquisition time for x-ray fluorescence imaging of gold nanoparticles: a preliminary study using photon counting detector

    NASA Astrophysics Data System (ADS)

    Ren, Liqiang; Wu, Di; Li, Yuhua; Chen, Wei R.; Zheng, Bin; Liu, Hong

    2016-03-01

    X-ray fluorescence (XRF) is a promising spectroscopic technique to characterize imaging contrast agents with high atomic numbers (Z) such as gold nanoparticles (GNPs) inside small objects. Its utilization for biomedical applications, however, is greatly limited to experimental research due to longer data acquisition time. The objectives of this study are to apply a photon counting detector array for XRF imaging and to determine an optimized XRF data acquisition time, at which the acquired XRF image is of acceptable quality to allow the maximum level of radiation dose reduction. A prototype laboratory XRF imaging configuration consisting of a pencil-beam X-ray and a photon counting detector array (1 × 64 pixels) is employed to acquire the XRF image through exciting the prepared GNP/water solutions. In order to analyze the signal to noise ratio (SNR) improvement versus the increased exposure time, all the XRF photons within the energy range of 63 - 76KeV that include two Kα gold fluorescence peaks are collected for 1s, 2s, 3s, and so on all the way up to 200s. The optimized XRF data acquisition time for imaging different GNP solutions is determined as the moment when the acquired XRF image just reaches a quality with a SNR of 20dB which corresponds to an acceptable image quality.

  1. Multispectral Imaging Broadens Cellular Analysis

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Amnis Corporation, a Seattle-based biotechnology company, developed ImageStream to produce sensitive fluorescence images of cells in flow. The company responded to an SBIR solicitation from Ames Research Center, and proposed to evaluate several methods of extending the depth of field for its ImageStream system and implement the best as an upgrade to its commercial products. This would allow users to view whole cells at the same time, rather than just one section of each cell. Through Phase I and II SBIR contracts, Ames provided Amnis the funding the company needed to develop this extended functionality. For NASA, the resulting high-speed image flow cytometry process made its way into Medusa, a life-detection instrument built to collect, store, and analyze sample organisms from erupting hydrothermal vents, and has the potential to benefit space flight health monitoring. On the commercial end, Amnis has implemented the process in ImageStream, combining high-resolution microscopy and flow cytometry in a single instrument, giving researchers the power to conduct quantitative analyses of individual cells and cell populations at the same time, in the same experiment. ImageStream is also built for many other applications, including cell signaling and pathway analysis; classification and characterization of peripheral blood mononuclear cell populations; quantitative morphology; apoptosis (cell death) assays; gene expression analysis; analysis of cell conjugates; molecular distribution; and receptor mapping and distribution.

  2. Cranial nerve assessment in posterior fossa tumors with fast imaging employing steady-state acquisition (FIESTA).

    PubMed

    Mikami, Takeshi; Minamida, Yoshihiro; Yamaki, Toshiaki; Koyanagi, Izumi; Nonaka, Tadashi; Houkin, Kiyohiro

    2005-10-01

    Steady-state free precession is widely used for ultra-fast cardiac or abdominal imaging. The purpose of this work was to assess fast imaging employing steady-state acquisition (FIESTA) and to evaluate its efficacy for depiction of the cranial nerve affected by the tumor. Twenty-three consecutive patients with posterior fossa tumors underwent FIESTA sequence after contrast agent administration, and then displacement of the cranial nerve was evaluated. The 23 patients with posterior fossa tumor consisted of 12 schwannomas, eight meningiomas, and three cases of epidermoid. Except in the cases of epidermoid, intensity of all tumors increased on FIESTA imaging of the contrast enhancement. In the schwannoma cases, visualization of the nerve became poorer as the tumor increased in size. In cases of encapsulated meningioma, all the cranial nerves of the posterior fossa were depicted regardless of location. The ability to depict the nerves was also significantly higher in meningioma patients than in schwannoma patients (P<0.05). In cases of epidermoid, extension of the tumors was depicted clearly. Although the FIESTA sequence offers similar contrast to other heavily T2-weighted sequences, it facilitated a superior assessment of the effect of tumors on cranial nerve anatomy. FIESTA sequence was useful for preoperative simulations of posterior fossa tumors.

  3. Photoacoustic pump-probe tomography of fluorophores in vivo using interleaved image acquisition for motion suppression

    NASA Astrophysics Data System (ADS)

    Märk, Julia; Wagener, Asja; Zhang, Edward; Laufer, Jan

    2017-01-01

    In fluorophores, the excited state lifetime can be modulated using pump-probe excitation. By generating photoacoustic (PA) signals using simultaneous and time-delayed pump and probe excitation pulses at fluences below the maximum permissible exposure, a modulation of the signal amplitude is observed in fluorophores but not in endogenous chromophores. This provides a highly specific contrast mechanism that can be used to recover the location of the fluorophore using difference imaging. The practical challenges in applying this method to in vivo PA tomography include the typically low concentrations of fluorescent contrast agents, and tissue motion. The former results in smaller PA signal amplitudes compared to those measured in blood, while the latter gives rise to difference image artefacts that compromise the unambiguous and potentially noise-limited detection of fluorescent contrast agents. To address this limitation, a method based on interleaved pump-probe image acquisition was developed. It relies on fast switching between simultaneous and time-delayed pump-probe excitation to acquire PA difference signals in quick succession, and to minimise the effects of tissue motion. The feasibility of this method is demonstrated in tissue phantoms and in initial experiments in vivo.

  4. Photoacoustic pump-probe tomography of fluorophores in vivo using interleaved image acquisition for motion suppression

    PubMed Central

    Märk, Julia; Wagener, Asja; Zhang, Edward; Laufer, Jan

    2017-01-01

    In fluorophores, the excited state lifetime can be modulated using pump-probe excitation. By generating photoacoustic (PA) signals using simultaneous and time-delayed pump and probe excitation pulses at fluences below the maximum permissible exposure, a modulation of the signal amplitude is observed in fluorophores but not in endogenous chromophores. This provides a highly specific contrast mechanism that can be used to recover the location of the fluorophore using difference imaging. The practical challenges in applying this method to in vivo PA tomography include the typically low concentrations of fluorescent contrast agents, and tissue motion. The former results in smaller PA signal amplitudes compared to those measured in blood, while the latter gives rise to difference image artefacts that compromise the unambiguous and potentially noise-limited detection of fluorescent contrast agents. To address this limitation, a method based on interleaved pump-probe image acquisition was developed. It relies on fast switching between simultaneous and time-delayed pump-probe excitation to acquire PA difference signals in quick succession, and to minimise the effects of tissue motion. The feasibility of this method is demonstrated in tissue phantoms and in initial experiments in vivo. PMID:28091571

  5. Recovery of phase inconsistencies in continuously moving table extended field of view magnetic resonance imaging acquisitions.

    PubMed

    Kruger, David G; Riederer, Stephen J; Rossman, Phillip J; Mostardi, Petrice M; Madhuranthakam, Ananth J; Hu, Houchun H

    2005-09-01

    MR images formed using extended FOV continuously moving table data acquisition can have signal falloff and loss of lateral spatial resolution at localized, periodic positions along the direction of table motion. In this work we identify the origin of these artifacts and provide a means for correction. The artifacts are due to a mismatch of the phase of signals acquired from contiguous sampling fields of view and are most pronounced when the central k-space views are being sampled. Correction can be performed using the phase information from a periodically sampled central view to adjust the phase of all other views of that view cycle, making the net phase uniform across each axial plane. Results from experimental phantom and contrast-enhanced peripheral MRA studies show that the correction technique substantially eliminates the artifact for a variety of phase encode orders.

  6. [EOS imaging acquisition system : 2D/3D diagnostics of the skeleton].

    PubMed

    Tarhan, T; Froemel, D; Meurer, A

    2015-12-01

    The application spectrum of the EOS imaging acquisition system is versatile. It is especially useful in the diagnostics and planning of corrective surgical procedures in complex orthopedic cases. The application is indicated when assessing deformities and malpositions of the spine, pelvis and lower extremities. It can also be used in the assessment and planning of hip and knee arthroplasty. For the first time physicians have the opportunity to conduct examinations of the whole body under weight-bearing conditions in order to anticipate the effects of a planned surgical procedure on the skeletal system as a whole and therefore on the posture of the patient. Compared to conventional radiographic examination techniques, such as x-ray or computed tomography, the patient is exposed to much less radiation. Therefore, the pediatric application of this technique can be described as reasonable.

  7. Phase incremented echo train acquisition applied to magnetic resonance pore imaging

    NASA Astrophysics Data System (ADS)

    Hertel, S. A.; Galvosas, P.

    2017-02-01

    Efficient phase cycling schemes remain a challenge for NMR techniques if the pulse sequences involve a large number of rf-pulses. Especially complex is the Carr Purcell Meiboom Gill (CPMG) pulse sequence where the number of rf-pulses can range from hundreds to several thousands. Our recent implementation of Magnetic Resonance Pore Imaging (MRPI) is based on a CPMG rf-pulse sequence in order to refocus the effect of internal gradients inherent in porous media. While the spin dynamics for spin- 1 / 2 systems in CPMG like experiments are well understood it is still not straight forward to separate the desired pathway from the spectrum of unwanted coherence pathways. In this contribution we apply Phase Incremented Echo Train Acquisition (PIETA) to MRPI. We show how PIETA offers a convenient way to implement a working phase cycling scheme and how it allows one to gain deeper insights into the amplitudes of undesired pathways.

  8. A digital receiver module with direct data acquisition for magnetic resonance imaging systems.

    PubMed

    Tang, Weinan; Sun, Hongyu; Wang, Weimin

    2012-10-01

    A digital receiver module for magnetic resonance imaging (MRI) with detailed hardware implementations is presented. The module is based on a direct sampling scheme using the latest mixed-signal circuit design techniques. A single field-programmable gate array chip is employed to perform software-based digital down conversion for radio frequency signals. The modular architecture of the receiver allows multiple acquisition channels to be implemented on a highly integrated printed circuit board. To maintain the phase coherence of the receiver and the exciter in the context of direct sampling, an effective phase synchronization method was proposed to achieve a phase deviation as small as 0.09°. The performance of the described receiver module was verified in the experiments for both low- and high-field (0.5 T and 1.5 T) MRI scanners and was compared to a modern commercial MRI receiver system.

  9. Results of shuttle EMU thermal vacuum tests incorporating an infrared imaging camera data acquisition system

    NASA Technical Reports Server (NTRS)

    Anderson, James E.; Tepper, Edward H.; Trevino, Louis A.

    1991-01-01

    Manned tests in Chamber B at NASA JSC were conducted in May and June of 1990 to better quantify the Space Shuttle Extravehicular Mobility Unit's (EMU) thermal performance in the cold environmental extremes of space. Use of an infrared imaging camera with real-time video monitoring of the output significantly added to the scope, quality and interpretation of the test conduct and data acquisition. Results of this test program have been effective in the thermal certification of a new insulation configuration and the '5000 Series' glove. In addition, the acceptable thermal performance of flight garments with visually deteriorated insulation was successfully demonstrated, thereby saving significant inspection and garment replacement cost. This test program also established a new method for collecting data vital to improving crew thermal comfort in a cold environment.

  10. Imaging study of a phase-sensitive breast-CT system in continuous acquisition mode

    NASA Astrophysics Data System (ADS)

    Delogu, P.; Golosio, B.; Fedon, C.; Arfelli, F.; Bellazzini, R.; Brez, A.; Brun, F.; Di Lillo, F.; Dreossi, D.; Mettivier, G.; Minuti, M.; Oliva, P.; Pichera, M.; Rigon, L.; Russo, P.; Sarno, A.; Spandre, G.; Tromba, G.; Longo, R.

    2017-01-01

    The SYRMA-CT project aims to set-up the first clinical trial of phase-contrast breast Computed Tomography with synchrotron radiation at the SYRMEP beamline of Elettra, the Italian synchrotron light source. The challenge in a dedicated breast CT is to match a high spatial resolution with a low dose level. In order to fulfil these requirements, the SYRMA-CT project uses a large area CdTe single photon counting detector (Pixirad-8), simultaneous algebraic reconstruction technique (SART) and phase retrieval pre-processing. This work investigates the imaging performances of the system in a continuous acquisition mode and with a low dose level towards the clinical application. A custom test object and a large surgical sample have been studied.

  11. Three-dimensional ultrasonic imaging of concrete elements using different SAFT data acquisition and processing schemes

    NASA Astrophysics Data System (ADS)

    Schickert, Martin

    2015-03-01

    Ultrasonic testing systems using transducer arrays and the SAFT (Synthetic Aperture Focusing Technique) reconstruction allow for imaging the internal structure of concrete elements. At one-sided access, three-dimensional representations of the concrete volume can be reconstructed in relatively great detail, permitting to detect and localize objects such as construction elements, built-in components, and flaws. Different SAFT data acquisition and processing schemes can be utilized which differ in terms of the measuring and computational effort and the reconstruction result. In this contribution, two methods are compared with respect to their principle of operation and their imaging characteristics. The first method is the conventional single-channel SAFT algorithm which is implemented using a virtual transducer that is moved within a transducer array by electronic switching. The second method is the Combinational SAFT algorithm (C-SAFT), also named Sampling Phased Array (SPA) or Full Matrix Capture/Total Focusing Method (TFM/FMC), which is realized using a combination of virtual transducers within a transducer array. Five variants of these two methods are compared by means of measurements obtained at test specimens containing objects typical of concrete elements. The automated SAFT imaging system FLEXUS is used for the measurements which includes a three-axis scanner with a 1.0 m × 0.8 m scan range and an electronically switched ultrasonic array consisting of 48 transducers in 16 groups. On the basis of two-dimensional and three-dimensional reconstructed images, qualitative and some quantitative results of the parameters image resolution, signal-to-noise ratio, measurement time, and computational effort are discussed in view of application characteristics of the SAFT variants.

  12. Acquisition of priori tissue optical structure based on non-rigid image registration

    NASA Astrophysics Data System (ADS)

    Wan, Wenbo; Li, Jiao; Liu, Lingling; Wang, Yihan; Zhang, Yan; Gao, Feng

    2015-03-01

    Shape-parameterized diffuse optical tomography (DOT), which is based on a priori that assumes the uniform distribution of the optical properties in the each region, shows the effectiveness of complex biological tissue optical heterogeneities reconstruction. The priori tissue optical structure could be acquired with the assistance of anatomical imaging methods such as X-ray computed tomography (XCT) which suffers from low-contrast for soft tissues including different optical characteristic regions. For the mouse model, a feasible strategy of a priori tissue optical structure acquisition is proposed based on a non-rigid image registration algorithm. During registration, a mapping matrix is calculated to elastically align the XCT image of reference mouse to the XCT image of target mouse. Applying the matrix to the reference atlas which is a detailed mesh of organs/tissues in reference mouse, registered atlas can be obtained as the anatomical structure of target mouse. By assigning the literature published optical parameters of each organ to the corresponding anatomical structure, optical structure of the target organism can be obtained as a priori information for DOT reconstruction algorithm. By applying the non-rigid image registration algorithm to a target mouse which is transformed from the reference mouse, the results show that the minimum correlation coefficient can be improved from 0.2781 (before registration) to 0.9032 (after fine registration), and the maximum average Euclid distances can be decreased from 12.80mm (before registration) to 1.02mm (after fine registration), which has verified the effectiveness of the algorithm.

  13. Three-dimensional ultrasonic imaging of concrete elements using different SAFT data acquisition and processing schemes

    SciTech Connect

    Schickert, Martin

    2015-03-31

    Ultrasonic testing systems using transducer arrays and the SAFT (Synthetic Aperture Focusing Technique) reconstruction allow for imaging the internal structure of concrete elements. At one-sided access, three-dimensional representations of the concrete volume can be reconstructed in relatively great detail, permitting to detect and localize objects such as construction elements, built-in components, and flaws. Different SAFT data acquisition and processing schemes can be utilized which differ in terms of the measuring and computational effort and the reconstruction result. In this contribution, two methods are compared with respect to their principle of operation and their imaging characteristics. The first method is the conventional single-channel SAFT algorithm which is implemented using a virtual transducer that is moved within a transducer array by electronic switching. The second method is the Combinational SAFT algorithm (C-SAFT), also named Sampling Phased Array (SPA) or Full Matrix Capture/Total Focusing Method (TFM/FMC), which is realized using a combination of virtual transducers within a transducer array. Five variants of these two methods are compared by means of measurements obtained at test specimens containing objects typical of concrete elements. The automated SAFT imaging system FLEXUS is used for the measurements which includes a three-axis scanner with a 1.0 m × 0.8 m scan range and an electronically switched ultrasonic array consisting of 48 transducers in 16 groups. On the basis of two-dimensional and three-dimensional reconstructed images, qualitative and some quantitative results of the parameters image resolution, signal-to-noise ratio, measurement time, and computational effort are discussed in view of application characteristics of the SAFT variants.

  14. Multivariate image analysis in biomedicine.

    PubMed

    Nattkemper, Tim W

    2004-10-01

    In recent years, multivariate imaging techniques are developed and applied in biomedical research in an increasing degree. In research projects and in clinical studies as well m-dimensional multivariate images (MVI) are recorded and stored to databases for a subsequent analysis. The complexity of the m-dimensional data and the growing number of high throughput applications call for new strategies for the application of image processing and data mining to support the direct interactive analysis by human experts. This article provides an overview of proposed approaches for MVI analysis in biomedicine. After summarizing the biomedical MVI techniques the two level framework for MVI analysis is illustrated. Following this framework, the state-of-the-art solutions from the fields of image processing and data mining are reviewed and discussed. Motivations for MVI data mining in biology and medicine are characterized, followed by an overview of graphical and auditory approaches for interactive data exploration. The paper concludes with summarizing open problems in MVI analysis and remarks upon the future development of biomedical MVI analysis.

  15. Autonomous image data reduction by analysis and interpretation

    NASA Technical Reports Server (NTRS)

    Eberlein, Susan; Yates, Gigi; Ritter, Niles

    1988-01-01

    Image data is a critical component of the scientific information acquired by space missions. Compression of image data is required due to the limited bandwidth of the data transmission channel and limited memory space on the acquisition vehicle. This need becomes more pressing when dealing with multispectral data where each pixel may comprise 300 or more bytes. An autonomous, real time, on-board image analysis system for an exploratory vehicle such as a Mars Rover is developed. The completed system will be capable of interpreting image data to produce reduced representations of the image, and of making decisions regarding the importance of data based on current scientific goals. Data from multiple sources, including stereo images, color images, and multispectral data, are fused into single image representations. Analysis techniques emphasize artificial neural networks. Clusters are described by their outlines and class values. These analysis and compression techniques are coupled with decision making capacity for determining importance of each image region. Areas determined to be noise or uninteresting can be discarded in favor of more important areas. Thus limited resources for data storage and transmission are allocated to the most significant images.

  16. Autonomous image data reduction by analysis and interpretation

    NASA Astrophysics Data System (ADS)

    Eberlein, Susan; Yates, Gigi; Ritter, Niles

    Image data is a critical component of the scientific information acquired by space missions. Compression of image data is required due to the limited bandwidth of the data transmission channel and limited memory space on the acquisition vehicle. This need becomes more pressing when dealing with multispectral data where each pixel may comprise 300 or more bytes. An autonomous, real time, on-board image analysis system for an exploratory vehicle such as a Mars Rover is developed. The completed system will be capable of interpreting image data to produce reduced representations of the image, and of making decisions regarding the importance of data based on current scientific goals. Data from multiple sources, including stereo images, color images, and multispectral data, are fused into single image representations. Analysis techniques emphasize artificial neural networks. Clusters are described by their outlines and class values. These analysis and compression techniques are coupled with decision-making capacity for determining importance of each image region. Areas determined to be noise or uninteresting can be discarded in favor of more important areas. Thus limited resources for data storage and transmission are allocated to the most significant images.

  17. Pattern Recognition Software and Techniques for Biological Image Analysis

    PubMed Central

    Shamir, Lior; Delaney, John D.; Orlov, Nikita; Eckley, D. Mark; Goldberg, Ilya G.

    2010-01-01

    The increasing prevalence of automated image acquisition systems is enabling new types of microscopy experiments that generate large image datasets. However, there is a perceived lack of robust image analysis systems required to process these diverse datasets. Most automated image analysis systems are tailored for specific types of microscopy, contrast methods, probes, and even cell types. This imposes significant constraints on experimental design, limiting their application to the narrow set of imaging methods for which they were designed. One of the approaches to address these limitations is pattern recognition, which was originally developed for remote sensing, and is increasingly being applied to the biology domain. This approach relies on training a computer to recognize patterns in images rather than developing algorithms or tuning parameters for specific image processing tasks. The generality of this approach promises to enable data mining in extensive image repositories, and provide objective and quantitative imaging assays for routine use. Here, we provide a brief overview of the technologies behind pattern recognition and its use in computer vision for biological and biomedical imaging. We list available software tools that can be used by biologists and suggest practical experimental considerations to make the best use of pattern recognition techniques for imaging assays. PMID:21124870

  18. Pattern recognition software and techniques for biological image analysis.

    PubMed

    Shamir, Lior; Delaney, John D; Orlov, Nikita; Eckley, D Mark; Goldberg, Ilya G

    2010-11-24

    The increasing prevalence of automated image acquisition systems is enabling new types of microscopy experiments that generate large image datasets. However, there is a perceived lack of robust image analysis systems required to process these diverse datasets. Most automated image analysis systems are tailored for specific types of microscopy, contrast methods, probes, and even cell types. This imposes significant constraints on experimental design, limiting their application to the narrow set of imaging methods for which they were designed. One of the approaches to address these limitations is pattern recognition, which was originally developed for remote sensing, and is increasingly being applied to the biology domain. This approach relies on training a computer to recognize patterns in images rather than developing algorithms or tuning parameters for specific image processing tasks. The generality of this approach promises to enable data mining in extensive image repositories, and provide objective and quantitative imaging assays for routine use. Here, we provide a brief overview of the technologies behind pattern recognition and its use in computer vision for biological and biomedical imaging. We list available software tools that can be used by biologists and suggest practical experimental considerations to make the best use of pattern recognition techniques for imaging assays.

  19. Thermal Imaging of the Waccasassa Bay Preserve: Image Acquisition and Processing

    USGS Publications Warehouse

    Raabe, Ellen A.; Bialkowska-Jelinska, Elzbieta

    2010-01-01

    Thermal infrared (TIR) imagery was acquired along coastal Levy County, Florida, in March 2009 with the goal of identifying groundwater-discharge locations in Waccasassa Bay Preserve State Park (WBPSP). Groundwater discharge is thermally distinct in winter when Floridan aquifer temperature, 71-72 degrees F, contrasts with the surrounding cold surface waters. Calibrated imagery was analyzed to assess temperature anomalies and related thermal traces. The influence of warm Gulf water and image artifacts on small features was successfully constrained by image evaluation in three separate zones: Creeks, Bay, and Gulf. Four levels of significant water-temperature anomalies were identified, and 488 sites of interest were mapped. Among the sites identified, at least 80 were determined to be associated with image artifacts and human activity, such as excavation pits and the Florida Barge Canal. Sites of interest were evaluated for geographic concentration and isolation. High site densities, indicating interconnectivity and prevailing flow, were located at Corrigan Reef, No. 4 Channel, Winzy Creek, Cow Creek, Withlacoochee River, and at excavation sites. In other areas, low to moderate site density indicates the presence of independent vents and unique flow paths. A directional distribution assessment of natural seep features produced a northwest trend closely matching the strike direction of regional faults. Naturally occurring seeps were located in karst ponds and tidal creeks, and several submerged sites were detected in Waccasassa River and Bay, representing the first documentation of submarine vents in the Waccasassa region. Drought conditions throughout the region placed constraints on positive feature identification. Low discharge or displacement by landward movement of saltwater may have reduced or reversed flow during this season. Approximately two-thirds of seep locations in the overlap between 2009 and 2005 TIR night imagery were positively re-identified in 2009

  20. The Wayside Mechanic: An Analysis of Skill Acquisition in Ghana.

    ERIC Educational Resources Information Center

    McLaughlin, Stephen Douglas

    This study describes and analyzes the nature of skill acquisition process in one indigenous, informal training system--the apprenticeship of the wayside mechanics workshops in Koforidua, Ghana. Chapter 2 places apprenticeships training in the wider context of artisanship and training. It traces the history of the West African craft shop and its…

  1. Strategic Acquisition of Navy Unmanned Systems: Analysis and Options

    DTIC Science & Technology

    2012-07-12

    UAS, with news stories reporting insatiable demand by operational commanders for UAS assets and Congress authorizing significant future UAS...distribution is unlimited. Prepared for: Naval Postgraduate School, Monterey, California 93943 NPS-AM-12-179 ACQUISITION RESEARCH SPONSORED REPORT ...Graduate School of Business & Public Policy Naval Postgraduate School Report Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting

  2. An Analysis of the Defense Acquisition Strategy for Unmanned Systems

    DTIC Science & Technology

    2014-03-01

    VHSIC Development .........................................................................34 3. RFID Development...product service code RAA Rapid Acquisition Authority RCS radar cross section REF Rapid Equipping Force RFID radio frequency identification RDT... RFID technology. 28 1. The Semiconductor Industry This section divides the early semiconductor industry history into three periods based on the

  3. Quantitative histogram analysis of images

    NASA Astrophysics Data System (ADS)

    Holub, Oliver; Ferreira, Sérgio T.

    2006-11-01

    A routine for histogram analysis of images has been written in the object-oriented, graphical development environment LabVIEW. The program converts an RGB bitmap image into an intensity-linear greyscale image according to selectable conversion coefficients. This greyscale image is subsequently analysed by plots of the intensity histogram and probability distribution of brightness, and by calculation of various parameters, including average brightness, standard deviation, variance, minimal and maximal brightness, mode, skewness and kurtosis of the histogram and the median of the probability distribution. The program allows interactive selection of specific regions of interest (ROI) in the image and definition of lower and upper threshold levels (e.g., to permit the removal of a constant background signal). The results of the analysis of multiple images can be conveniently saved and exported for plotting in other programs, which allows fast analysis of relatively large sets of image data. The program file accompanies this manuscript together with a detailed description of two application examples: The analysis of fluorescence microscopy images, specifically of tau-immunofluorescence in primary cultures of rat cortical and hippocampal neurons, and the quantification of protein bands by Western-blot. The possibilities and limitations of this kind of analysis are discussed. Program summaryTitle of program: HAWGC Catalogue identifier: ADXG_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/ADXG_v1_0 Program obtainable from: CPC Program Library, Queen's University of Belfast, N. Ireland Computers: Mobile Intel Pentium III, AMD Duron Installations: No installation necessary—Executable file together with necessary files for LabVIEW Run-time engine Operating systems or monitors under which the program has been tested: WindowsME/2000/XP Programming language used: LabVIEW 7.0 Memory required to execute with typical data:˜16MB for starting and ˜160MB used for

  4. Quantitative FRET Analysis by Fast Acquisition Time Domain FLIM at High Spatial Resolution in Living Cells

    PubMed Central

    Padilla-Parra, Sergi; Audugé, Nicolas; Coppey-Moisan, Maïté; Tramier, Marc

    2008-01-01

    Quantitative analysis in Förster resonance energy transfer (FRET) experiments in live cells for protein interaction studies is still a challenging issue. In a two-component system (FRET and no FRET donor species), fitting of fluorescence lifetime imaging microscopy (FLIM) data gives the fraction of donor molecules involved in FRET (fD) and the intrinsic transfer efficiency. But when fast FLIM acquisitions are used to monitor dynamic changes in protein-protein interactions at high spatial and temporal resolutions in living cells, photon statistics and time resolution are limited. In this case, fitting procedures are not reliable, even for single lifetime donors. We introduce the new concept of a minimal fraction of donor molecules involved in FRET (mfD), coming from the mathematical minimization of fD. We find particular advantage in the use of mfD because it can be obtained without fitting procedures and it is derived directly from FLIM data. mfD constitutes an interesting quantitative parameter for live cell studies because it is related to the minimal relative concentration of interacting proteins. For multi-lifetime donors, the process of fitting complex fluorescence decays to find at least four reliable lifetimes is a near impossible task. Here, mfD extension for multi-lifetime donors is the only quantitative determinant. We applied this methodology for imaging the interaction between the bromodomains of TAFII250 and acetylated histones H4 in living cells at high resolution. We show the existence of discrete acetylated chromatin domains where the minimal fraction of bromodomain interacting with acetylated H4 oscillates from 0.26 to 0.36 and whose size is smaller than half of one micron cube. We demonstrate that mfD by itself is a useful tool to investigate quantitatively protein interactions in live cells, especially when using fast FRET-FLIM acquisition times. PMID:18539634

  5. From chaos to order: The MicroStar data acquisition and analysis system

    SciTech Connect

    Rathbun, W.

    1991-03-01

    The MicroStar data acquisition and analysis software consists of several independent processes, although to the user it looks like a single program. These programs handle such functions as data acquisition (if any), data analysis, interactive display and data manipulation, and process monitoring and control. An overview of these processes and functions can be found in the paper as well as a more detailed description and users' guide.

  6. Lexical Link Analysis (LLA) Application: Improving Web Service to Defense Acquisition Visibility Environment

    DTIC Science & Technology

    2015-09-30

    SPONSORED REPORT SERIES Lexical Link Analysis (LLA) Application: Improving Web Service to Defense Acquisition Visibility Environment 30...Lexical Link Analysis (LLA) Application: Improving Web Service to Defense Acquisition Visibility Environment 30 September 2015 Dr. Ying Zhao, Research...tasks completed this year. Task 1. We worked with the OSD OUSD ATL (US) to install the LLA/SSA/CLA system as a web service in the Defense

  7. Calibration of a flood inundation model using a SAR image: influence of acquisition time

    NASA Astrophysics Data System (ADS)

    Van Wesemael, Alexandra; Gobeyn, Sacha; Neal, Jeffrey; Lievens, Hans; Van Eerdenbrugh, Katrien; De Vleeschouwer, Niels; Schumann, Guy; Vernieuwe, Hilde; Di Baldassarre, Giuliano; De Baets, Bernard; Bates, Paul; Verhoest, Niko

    2016-04-01

    Flood risk management has always been in a search for effective prediction approaches. As such, the calibration of flood inundation models is continuously improved. In practice, this calibration process consists of finding the optimal roughness parameters, both channel and floodplain Manning coefficients, since these values considerably influence the flood extent in a catchment. In addition, Synthetic Aperture Radar (SAR) images have been proven to be a very useful tool in calibrating the flood extent. These images can distinguish between wet (flooded) and dry (non-flooded) pixels through the intensity of backscattered radio waves. To this date, however, satellite overpass often occurs only once during a flood event. Therefore, this study is specifically concerned with the effect of the timing of the SAR data acquisition on calibration results. In order to model the flood extent, the raster-based inundation model, LISFLOOD-FP, is used together with a high resolution synthetic aperture radar image (ERS-2 SAR) of a flood event of the river Dee, Wales, in December 2006. As only one satellite image of the considered case study is available, a synthetic framework is implemented in order to generate a time series of SAR observations. These synthetic observations are then used to calibrate the model at different time instants. In doing so, the sensitivity of the model output to the channel and floodplain Manning coefficients is studied through time. As results are examined, these suggest that there is a clear difference in the spatial variability to which water is held within the floodplain. Furthermore, these differences seem to be variable through time. Calibration by means of satellite flood observations obtained from the rising or receding limb, would generally lead to more reliable results rather than near peak flow observations.

  8. Whole-slide imaging and automated image analysis: considerations and opportunities in the practice of pathology.

    PubMed

    Webster, J D; Dunstan, R W

    2014-01-01

    Digital pathology, the practice of pathology using digitized images of pathologic specimens, has been transformed in recent years by the development of whole-slide imaging systems, which allow for the evaluation and interpretation of digital images of entire histologic sections. Applications of whole-slide imaging include rapid transmission of pathologic data for consultations and collaborations, standardization and distribution of pathologic materials for education, tissue specimen archiving, and image analysis of histologic specimens. Histologic image analysis allows for the acquisition of objective measurements of histomorphologic, histochemical, and immunohistochemical properties of tissue sections, increasing both the quantity and quality of data obtained from histologic assessments. Currently, numerous histologic image analysis software solutions are commercially available. Choosing the appropriate solution is dependent on considerations of the investigative question, computer programming and image analysis expertise, and cost. However, all studies using histologic image analysis require careful consideration of preanalytical variables, such as tissue collection, fixation, and processing, and experimental design, including sample selection, controls, reference standards, and the variables being measured. The fields of digital pathology and histologic image analysis are continuing to evolve, and their potential impact on pathology is still growing. These methodologies will increasingly transform the practice of pathology, allowing it to mature toward a quantitative science. However, this maturation requires pathologists to be at the forefront of the process, ensuring their appropriate application and the validity of their results. Therefore, histologic image analysis and the field of pathology should co-evolve, creating a symbiotic relationship that results in high-quality reproducible, objective data.

  9. Performance of the front end electronics and data acquisition system for the SLD Cherenkov Ring Imaging Detector

    SciTech Connect

    Abe, K.; Hasegawa, K.; Suekane, F.; Yuta, H. . Dept. of Physics); Antilogus, P.; Aston, D.; Bienz, T.; Bird, F.; Dasu, S.; Dolinsky, S.; Dunwoodie, W.; Hallewell, G.; Hoeflich, J.; Kawahara, H.; Kwon, Y.; Leith, D.W.G.S.; Marshall, D.; Muller, D.; Nagamine, T.; Oxoby, G.; Pavel, T.J.; Ratcliff, B.; Rensing, P.; Schultz, D.; Shapiro, S.; Simopoulos, C.; Solodov, E.; Stiles, P.; Toge, N.; Va'vra, J

    1991-11-01

    The front end electronics and data acquisition system for the SLD barrel Cherenkov Ring Imaging Detector (CRID) are described. This electronics must provide a 1% charge division measurement with a maximum acceptable noise level of 2000 electrons (rms). Noise and system performance results are presented for the initial SLD engineering run data.

  10. Revisiting age-of-acquisition effects in Spanish visual word recognition: the role of item imageability.

    PubMed

    Wilson, Maximiliano A; Cuetos, Fernando; Davies, Rob; Burani, Cristina

    2013-11-01

    Word age-of-acquisition (AoA) affects reading. The mapping hypothesis predicts AoA effects when input-output mappings are arbitrary. In Spanish, the orthography-to-phonology mappings required for word naming are consistent; therefore, no AoA effects are expected. Nevertheless, AoA effects have been found, motivating the present investigation of how AoA can affect reading in Spanish. Four experiments were run to examine reading with a factorial design manipulating AoA and frequency. In Experiments 1 and 2 (immediate and speeded naming), only word frequency affected word naming. In Experiment 3 (lexical decision), both AoA and frequency affected word recognition. In Experiment 4 (immediate naming with highly imageable items), both frequency and AoA affected naming. The results suggest that highly imageable items induce a larger reliance on semantics in reading aloud. Such reliance causes faster naming of earlier acquired words because the corresponding concepts have richer visual and sensory features acquired mainly through direct sensory experience.

  11. Lock Acquisition and Sensitivity Analysis of Advanced LIGO Interferometers

    NASA Astrophysics Data System (ADS)

    Martynov, Denis

    Laser interferometer gravitational wave observatory (LIGO) consists of two complex large-scale laser interferometers designed for direct detection of gravitational waves from distant astrophysical sources in the frequency range 10Hz - 5kHz. Direct detection of space-time ripples will support Einstein's general theory of relativity and provide invaluable information and new insight into physics of the Universe. The initial phase of LIGO started in 2002, and since then data was collected during the six science runs. Instrument sensitivity improved from run to run due to the effort of commissioning team. Initial LIGO has reached designed sensitivity during the last science run, which ended in October 2010. In parallel with commissioning and data analysis with the initial detector, LIGO group worked on research and development of the next generation of detectors. Major instrument upgrade from initial to advanced LIGO started in 2010 and lasted until 2014. This thesis describes results of commissioning work done at the LIGO Livingston site from 2013 until 2015 in parallel with and after the installation of the instrument. This thesis also discusses new techniques and tools developed at the 40m prototype including adaptive filtering, estimation of quantization noise in digital filters and design of isolation kits for ground seismometers. The first part of this thesis is devoted to the description of methods for bringing the interferometer into linear regime when collection of data becomes possible. States of longitudinal and angular controls of interferometer degrees of freedom during lock acquisition process and in low noise configuration are discussed in details. Once interferometer is locked and transitioned to low noise regime, instrument produces astrophysics data that should be calibrated to units of meters or strain. The second part of this thesis describes online calibration technique set up in both observatories to monitor the quality of the collected data in

  12. A Unified Mathematical Approach to Image Analysis.

    DTIC Science & Technology

    1987-08-31

    describes four instances of the paradigm in detail. Directions for ongoing and future research are also indicated. Keywords: Image processing; Algorithms; Segmentation; Boundary detection; tomography; Global image analysis .

  13. Defense Acquisition Reform: Background, Analysis, and Issues for Congress

    DTIC Science & Technology

    2014-05-23

    MIT professor Harvey Sapolsky wrote The limited number of available reforms have all been recycled. You can centralize or decentralize. You can...Aircraft Carrier Program: Background and Issues for Congress, by Ronald O’Rourke, p. 9. 16 Harvey Sapolsky, “Let’s Skip Acquisition Reform This Time...challenges of an ever changing landscape and that fundamental reforms are necessary.42 In 2009, Norman Augustine (former CEO of Lockheed Martin) and

  14. Quantum dot imaging in the second near-infrared optical window: studies on reflectance fluorescence imaging depths by effective fluence rate and multiple image acquisition

    NASA Astrophysics Data System (ADS)

    Jung, Yebin; Jeong, Sanghwa; Nayoun, Won; Ahn, Boeun; Kwag, Jungheon; Geol Kim, Sang; Kim, Sungjee

    2015-04-01

    Quantum dot (QD) imaging capability was investigated by the imaging depth at a near-infrared second optical window (SOW; 1000 to 1400 nm) using time-modulated pulsed laser excitations to control the effective fluence rate. Various media, such as liquid phantoms, tissues, and in vivo small animals, were used and the imaging depths were compared with our predicted values. The QD imaging depth under excitation of continuous 20 mW/cm2 laser was determined to be 10.3 mm for 2 wt% hemoglobin phantom medium and 5.85 mm for 1 wt% intralipid phantom, which were extended by more than two times on increasing the effective fluence rate to 2000 mW/cm2. Bovine liver and porcine skin tissues also showed similar enhancement in the contrast-to-noise ratio (CNR) values. A QD sample was inserted into the abdomen of a mouse. With a higher effective fluence rate, the CNR increased more than twofold and the QD sample became clearly visualized, which was completely undetectable under continuous excitation. Multiple acquisitions of QD images and averaging process pixel by pixel were performed to overcome the thermal noise issue of the detector in SOW, which yielded significant enhancement in the imaging capability, showing up to a 1.5 times increase in the CNR.

  15. Trajectory analysis for magnetic particle imaging.

    PubMed

    Knopp, T; Biederer, S; Sattel, T; Weizenecker, J; Gleich, B; Borgert, J; Buzug, T M

    2009-01-21

    Recently a new imaging technique called magnetic particle imaging was proposed. The method uses the nonlinear response of magnetic nanoparticles when a time varying magnetic field is applied. Spatial encoding is achieved by moving a field-free point through an object of interest while the field strength in the vicinity of the point is high. A resolution in the submillimeter range is provided even for fast data acquisition sequences. In this paper, a simulation study is performed on different trajectories moving the field-free point through the field of view. The purpose is to provide mandatory information for the design of a magnetic particle imaging scanner. Trajectories are compared with respect to density, speed and image quality when applied in data acquisition. Since simulation of the involved physics is a time demanding task, moreover, an efficient implementation is presented utilizing caching techniques.

  16. Reduction and analysis techniques for infrared imaging data

    NASA Technical Reports Server (NTRS)

    Mccaughrean, Mark

    1989-01-01

    Infrared detector arrays are becoming increasingly available to the astronomy community, with a number of array cameras already in use at national observatories, and others under development at many institutions. As the detector technology and imaging instruments grow more sophisticated, more attention is focussed on the business of turning raw data into scientifically significant information. Turning pictures into papers, or equivalently, astronomy into astrophysics, both accurately and efficiently, is discussed. Also discussed are some of the factors that can be considered at each of three major stages; acquisition, reduction, and analysis, concentrating in particular on several of the questions most relevant to the techniques currently applied to near infrared imaging.

  17. Segmentation and learning in the quantitative analysis of microscopy images

    NASA Astrophysics Data System (ADS)

    Ruggiero, Christy; Ross, Amy; Porter, Reid

    2015-02-01

    In material science and bio-medical domains the quantity and quality of microscopy images is rapidly increasing and there is a great need to automatically detect, delineate and quantify particles, grains, cells, neurons and other functional "objects" within these images. These are challenging problems for image processing because of the variability in object appearance that inevitably arises in real world image acquisition and analysis. One of the most promising (and practical) ways to address these challenges is interactive image segmentation. These algorithms are designed to incorporate input from a human operator to tailor the segmentation method to the image at hand. Interactive image segmentation is now a key tool in a wide range of applications in microscopy and elsewhere. Historically, interactive image segmentation algorithms have tailored segmentation on an image-by-image basis, and information derived from operator input is not transferred between images. But recently there has been increasing interest to use machine learning in segmentation to provide interactive tools that accumulate and learn from the operator input over longer periods of time. These new learning algorithms reduce the need for operator input over time, and can potentially provide a more dynamic balance between customization and automation for different applications. This paper reviews the state of the art in this area, provides a unified view of these algorithms, and compares the segmentation performance of various design choices.

  18. Acquisition of Flexible Image Recognition by Coupling of Reinforcement Learning and a Neural Network

    NASA Astrophysics Data System (ADS)

    Shibata, Katsunari; Kawano, Tomohiko

    The authors have proposed a very simple autonomous learning system consisting of one neural network (NN), whose inputs are raw sensor signals and whose outputs are directly passed to actuators as control signals, and which is trained by using reinforcement learning (RL). However, the current opinion seems that such simple learning systems do not actually work on complicated tasks in the real world. In this paper, with a view to developing higher functions in robots, the authors bring up the necessity to introduce autonomous learning of a massively parallel and cohesively flexible system with massive inputs based on the consideration about the brain architecture and the sequential property of our consciousness. The authors also bring up the necessity to place more importance on “optimization” of the total system under a uniform criterion than “understandability” for humans. Thus, the authors attempt to stress the importance of their proposed system when considering the future research on robot intelligence. The experimental result in a real-world-like environment shows that image recognition from as many as 6240 visual signals can be acquired through RL under various backgrounds and light conditions without providing any knowledge about image processing or the target object. It works even for camera image inputs that were not experienced in learning. In the hidden layer, template-like representation, division of roles between hidden neurons, and representation to detect the target uninfluenced by light condition or background were observed after learning. The autonomous acquisition of such useful representations or functions makes us feel the potential towards avoidance of the frame problem and the development of higher functions.

  19. Novel ultrahigh resolution data acquisition and image reconstruction for multi-detector row CT

    SciTech Connect

    Flohr, T. G.; Stierstorfer, K.; Suess, C.; Schmidt, B.; Primak, A. N.; McCollough, C. H.

    2007-05-15

    We present and evaluate a special ultrahigh resolution mode providing considerably enhanced spatial resolution both in the scan plane and in the z-axis direction for a routine medical multi-detector row computed tomography (CT) system. Data acquisition is performed by using a flying focal spot both in the scan plane and in the z-axis direction in combination with tantalum grids that are inserted in front of the multi-row detector to reduce the aperture of the detector elements both in-plane and in the z-axis direction. The dose utilization of the system for standard applications is not affected, since the grids are moved into place only when needed and are removed for standard scanning. By means of this technique, image slices with a nominal section width of 0.4 mm (measured full width at half maximum=0.45 mm) can be reconstructed in spiral mode on a CT system with a detector configuration of 32x0.6 mm. The measured 2% value of the in-plane modulation transfer function (MTF) is 20.4 lp/cm, the measured 2% value of the longitudinal (z axis) MTF is 21.5 lp/cm. In a resolution phantom with metal line pair test patterns, spatial resolution of 20 lp/cm can be demonstrated both in the scan plane and along the z axis. This corresponds to an object size of 0.25 mm that can be resolved. The new mode is intended for ultrahigh resolution bone imaging, in particular for wrists, joints, and inner ear studies, where a higher level of image noise due to the reduced aperture is an acceptable trade-off for the clinical benefit brought about by the improved spatial resolution.

  20. The 2014 Broadband Acquisition and Imaging Operation (BAcIO) at Stromboli Volcano (Italy)

    NASA Astrophysics Data System (ADS)

    Scarlato, P.; Taddeucci, J.; Del Bello, E.; Gaudin, D.; Ricci, T.; Andronico, D.; Lodato, L.; Cannata, A.; Ferrari, F.; Orr, T. R.; Sesterhenn, J.; Plescher, R.; Baumgärtel, Y.; Harris, A. J. L.; Bombrun, M.; Barnie, T. D.; Houghton, B. F.; Kueppers, U.; Capponi, A.

    2014-12-01

    In May 2014, Stromboli volcano, one of the best natural laboratories for the study of weak explosive volcanism, hosted a large combination of state-of-the-art and prototype eruption monitoring technologies. Aiming to expand our parameterization capabilities for explosive eruption dynamics, we temporarily deployed in direct view of the active vents a range of imaging, acoustic, and seismic data acquisition systems. Imaging systems included: two high-speed visible cameras acquiring synchronized images at 500 and 1000 frames per second (fps); two thermal infrared forward looking (FLIR) cameras zooming into the active vents and acquiring at 50-200 fps; two FLIR cameras acquiring at lower (3-50 fps) frame rates with a broader field of view; one visible time-lapse camera; one UV camera system for the measurement of sulphur dioxide emission; and one drone equipped with a camcorder. Acoustic systems included: four broadband microphones (range of tens of kHz to 0.1 Hz), two of them co-located with one of the high-speed cameras and one co-located with one of the seismometers (see below); and an acoustic microphone array. This array included sixteen microphones with a circular arrangement located on a steep slope above the active vents. Seismic systems included two broadband seismometers, one of them co-located with one of the high-speed cameras, and one co-located with one of the microphones. The above systems were synchronized with a variety of methods, and temporarily added to the permanent monitoring networks already operating on the island. Observation focus was on pyroclast ejection processes extending from the shallow conduit, through their acceleration and interaction with the atmosphere, and to their dispersal and deposition. The 3-D distribution of bombs, the sources of jet noise in the explosions, the comparison between methods for estimating explosion properties, and the relations between erupted gas and magma volumes, are some examples of the processes targeted

  1. Seismic acquisition parameters analysis for deep weak reflectors in the South Yellow Sea

    NASA Astrophysics Data System (ADS)

    Liu, Kai; Liu, Huaishan; Wu, Zhiqiang; Yue, Long

    2016-10-01

    The Mesozoic-Paleozoic marine residual basin in the South Yellow Sea (SYS) is a significant deep potential hydrocarbon reservoir. However, the imaging of the deep prospecting target is quite challenging due to the specific seismic-geological conditions. In the Central and Wunansha Uplifts, the penetration of the seismic wavefield is limited by the shallow high-velocity layers (HVLs) and the weak reflections in the deep carbonate rocks. With the conventional marine seismic acquisition technique, the deep weak reflection is difficult to image and identify. In this paper, we could confirm through numerical simulation that the combination of multi-level air-gun array and extended cable used in the seismic acquisition is crucial for improving the imaging quality. Based on the velocity model derived from the geological interpretation, we performed two-dimensional finite difference forward modeling. The numerical simulation results show that the use of the multi-level air-gun array can enhance low-frequency energy and that the wide-angle reflection received at far offsets of the extended cable has a higher signal-to-noise ratio (SNR) and higher energy. Therefore, we have demonstrated that the unconventional wide-angle seismic acquisition technique mentioned above could overcome the difficulty in imaging the deep weak reflectors of the SYS, and it may be useful for the design of practical seismic acquisition schemes in this region.

  2. Super-resolution reconstruction to increase the spatial resolution of diffusion weighted images from orthogonal anisotropic acquisitions

    PubMed Central

    Scherrer, Benoit; Gholipour, Ali; Warfield, Simon K.

    2012-01-01

    Diffusion-weighted imaging (DWI) enables non-invasive investigation and characterization of the white matter but suffers from a relatively poor spatial resolution. Increasing the spatial resolution in DWI is challenging with a single-shot EPI acquisition due to the decreased signal-to-noise ratio and T2* relaxation effect amplified with increased echo time. In this work we propose a super-resolution reconstruction (SRR) technique based on the acquisition of multiple anisotropic orthogonal DWI scans. DWI scans acquired in different planes are not typically closely aligned due to the geometric distortion introduced by magnetic susceptibility differences in each phase-encoding direction. We compensate each scan for geometric distortion by acquisition of a dual echo gradient echo field map, providing an estimate of the field inhomogeneity. We address the problem of patient motion by aligning the volumes in both space and q-space. The SRR is formulated as a maximum a posteriori problem. It relies on a volume acquisition model which describes how the acquired scans are observations of an unknown high-resolution image which we aim to recover. Our model enables the introduction of image priors that exploit spatial homogeneity and enables regularized solutions. We detail our SRR optimization procedure and report experiments including numerical simulations, synthetic SRR and real world SRR. In particular, we demonstrate that combining distortion compensation and SRR provides better results than acquisition of a single isotropic scan for the same acquisition duration time. Importantly, SRR enables DWI with resolution beyond the scanner hardware limitations. This work provides the first evidence that SRR, which employs conventional single shot EPI techniques, enables resolution enhancement in DWI, and may dramatically impact the role of DWI in both neuroscience and clinical applications. PMID:22770597

  3. Three-dimensional fast imaging employing steady-state acquisition MRI and its diagnostic value for lumbar foraminal stenosis.

    PubMed

    Nemoto, Osamu; Fujikawa, Akira; Tachibana, Atsuko

    2014-07-01

    The aim of this study was to evaluate the usefulness of three-dimensional (3D) fast imaging employing steady-state acquisition (3D FIESTA) in the diagnosis of lumbar foraminal stenosis (LFS). Fifteen patients with LFS and 10 healthy volunteers were studied. All patients met the following criteria: (1) single L5 radiculopathy without compressive lesion in the spinal canal, (2) pain reproduction during provocative radiculography, and (3) improvement of symptoms after surgery. We retrospectively compared the symptomatic nerve roots to the asymptomatic nerve roots on fast spin-echo (FSE) T1 sagittal, FSE T2 axial and reconstituted 3D FIESTA images. The κ values for interobserver agreement in determining the presence of LFS were 0.525 for FSE T1 sagittal images, 0.735 for FSE T2 axial images, 0.750 for 3D FIESTA sagittal, 0.733 for axial images, and 0.953 for coronal images. The sensitivities and specificities were 60 and 86 % for FSE T1 sagittal images, 27 and 91 % for FSE T2 axial images, 60 and 97 % for 3D FIESTA sagittal images, 60 and 94 % for 3D FIESTA axial images, and 100 and 97 % for 3D FIESTA coronal images, respectively. 3D FIESTA can provide more reliable and additional information for the running course of lumbar nerve root, compared with conventional magnetic resonance imaging. Particularly, use of 3D FIESTA coronal images enables accurate diagnosis for LFS.

  4. Image analysis in medical imaging: recent advances in selected examples.

    PubMed

    Dougherty, G

    2010-01-01

    Medical imaging has developed into one of the most important fields within scientific imaging due to the rapid and continuing progress in computerised medical image visualisation and advances in analysis methods and computer-aided diagnosis. Several research applications are selected to illustrate the advances in image analysis algorithms and visualisation. Recent results, including previously unpublished data, are presented to illustrate the challenges and ongoing developments.

  5. An Analysis of the Support Equipment Acquisition Process and Methods Designed to Reduce Acquisition Leadtime

    DTIC Science & Technology

    1991-09-01

    For !4TIs GRA& I DTIC TAB 1-j. O urn~nc Qd D1 ."JuJt I r:. % +I 1Avajll 1c/or. Distv , Li AFIT/GLM/LSY/91S-68 AN ANALYSIS OF THE SUPPORT EQUIPMENT...a specific function or purpose (21:10). Contractor Furnished Equipment ( CFE ). Items acquired or manufactured directly by the contractor for use in the...process, or output: 1) inputs are those items or resources used by the system which allow iL o func 4 ion ; 2) processes or transforms inputs into outputs

  6. Lexical Link Analysis (LLA) Application: Improving Web Service to Defense Acquisition Visibility Environment (DAVE)

    DTIC Science & Technology

    2015-05-01

    1 LEXICAL LINK ANALYSIS (LLA) APPLICATION: IMPROVING WEB SERVICE TO DEFENSE ACQUISITION VISIBILITY ENVIRONMENT(DAVE) May 13-14, 2015 Dr. Ying...Improving Web Service to Defense Acquisition Visibility Environment (DAVE) 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR...pattern recognition that scales up to Big Data • System Self-Awareness (SSA) • Big data and Deep Learning (BDDL) / Big Data Architecture and

  7. The Department of Defense Acquisition Workforce: Background, Analysis, and Questions for Congress

    DTIC Science & Technology

    2016-07-29

    retention of acquisition personnel by, respectively, establishing (1) professional development requirements, (2) monetary incentives and accelerated hiring...RAND, An Analysis of Personnel Trends Relevant to Policy , 1993-2006, Santa Monica, CA, 2008, p. 2. The Department of Defense Acquisition Workforce...Department of Defense Financial Management Policy and Procedures, DOD Instruction 7000.14-R, at http://comptroller.defense.gov/fmr.aspx. 15 U.S

  8. Quantitative multi-image analysis for biomedical Raman spectroscopic imaging.

    PubMed

    Hedegaard, Martin A B; Bergholt, Mads S; Stevens, Molly M

    2016-05-01

    Imaging by Raman spectroscopy enables unparalleled label-free insights into cell and tissue composition at the molecular level. With established approaches limited to single image analysis, there are currently no general guidelines or consensus on how to quantify biochemical components across multiple Raman images. Here, we describe a broadly applicable methodology for the combination of multiple Raman images into a single image for analysis. This is achieved by removing image specific background interference, unfolding the series of Raman images into a single dataset, and normalisation of each Raman spectrum to render comparable Raman images. Multivariate image analysis is finally applied to derive the contributing 'pure' biochemical spectra for relative quantification. We present our methodology using four independently measured Raman images of control cells and four images of cells treated with strontium ions from substituted bioactive glass. We show that the relative biochemical distribution per area of the cells can be quantified. In addition, using k-means clustering, we are able to discriminate between the two cell types over multiple Raman images. This study shows a streamlined quantitative multi-image analysis tool for improving cell/tissue characterisation and opens new avenues in biomedical Raman spectroscopic imaging.

  9. MIRAGE: The data acquisition, analysis, and display system

    NASA Technical Reports Server (NTRS)

    Rosser, Robert S.; Rahman, Hasan H.

    1993-01-01

    Developed for the NASA Johnson Space Center and Life Sciences Directorate by GE Government Services, the Microcomputer Integrated Real-time Acquisition Ground Equipment (MIRAGE) system is a portable ground support system for Spacelab life sciences experiments. The MIRAGE system can acquire digital or analog data. Digital data may be NRZ-formatted telemetry packets of packets from a network interface. Analog signal are digitized and stored in experimental packet format. Data packets from any acquisition source are archived to a disk as they are received. Meta-parameters are generated from the data packet parameters by applying mathematical and logical operators. Parameters are displayed in text and graphical form or output to analog devices. Experiment data packets may be retransmitted through the network interface. Data stream definition, experiment parameter format, parameter displays, and other variables are configured using spreadsheet database. A database can be developed to support virtually any data packet format. The user interface provides menu- and icon-driven program control. The MIRAGE system can be integrated with other workstations to perform a variety of functions. The generic capabilities, adaptability and ease of use make the MIRAGE a cost-effective solution to many experimental data processing requirements.

  10. Optical hyperspectral imaging in microscopy and spectroscopy – a review of data acquisition

    PubMed Central

    Gao, Liang; Smith, R. Theodore

    2014-01-01

    Rather than simply acting as a photographic camera capturing two-dimensional (x, y) intensity images or a spectrometer acquiring spectra (λ), a hyperspectral imager measures entire three-dimensional (x, y, λ) datacubes for multivariate analysis, providing structural, molecular, and functional information about biological cells or tissue with unprecedented detail. Such data also gives clinical insights for disease diagnosis and treatment. We summarize the principles underpinning this technology, highlight its practical implementation, and discuss its recent applications at microscopic to macroscopic scales. PMID:25186815

  11. Imaging analysis of LDEF craters

    NASA Technical Reports Server (NTRS)

    Radicatidibrozolo, F.; Harris, D. W.; Chakel, J. A.; Fleming, R. H.; Bunch, T. E.

    1991-01-01

    Two small craters in Al from the Long Duration Exposure Facility (LDEF) experiment tray A11E00F (no. 74, 119 micron diameter and no. 31, 158 micron diameter) were analyzed using Auger electron spectroscopy (AES), time-of-flight secondary ion mass spectroscopy (TOF-SIMS), low voltage scanning electron microscopy (LVSEM), and SEM energy dispersive spectroscopy (EDS). High resolution images and sensitive elemental and molecular analysis were obtained with this combined approach. The result of these analyses are presented.

  12. Performance evaluation of real-simulated image matching techniques in the acquisition of ground control for ERS-1 image geocoding

    NASA Astrophysics Data System (ADS)

    Guidon, B.

    Recently, a large-area digital ERS-1 image mosaic was generated of a mountainous region of British Columbia, Canada. Ground control for the geocoding of each of the 53 constituent scenes was derived by matching real and simulated image chips, the latter generated from digital elevation models (DEMs) and a knowledge of the ERS-1 imaging configuration. This paper presents an analysis of automated and manual matching performance based on a study of the aggregate data set of 1270 control points (CPs). The CP selection methodology is described as well as the relationship between matching accuracy and the level of goodness-of-match as quantified by the Spearman rank correlation coefficient. A statistical approach is proposed for the selection of an optimum correlation threshold for operational geocoding scenarios.

  13. Image analysis for denoising full-field frequency-domain fluorescence lifetime images.

    PubMed

    Spring, B Q; Clegg, R M

    2009-08-01

    Video-rate fluorescence lifetime-resolved imaging microscopy (FLIM) is a quantitative imaging technique for measuring dynamic processes in biological specimens. FLIM offers valuable information in addition to simple fluorescence intensity imaging; for instance, the fluorescence lifetime is sensitive to the microenvironment of the fluorophore allowing reliable differentiation between concentration differences and dynamic quenching. Homodyne FLIM is a full-field frequency-domain technique for imaging fluorescence lifetimes at every pixel of a fluorescence image simultaneously. If a single modulation frequency is used, video-rate image acquisition is possible. Homodyne FLIM uses a gain-modulated image intensified charge-coupled device (ICCD) detector, which unfortunately is a major contribution to the noise of the measurement. Here we introduce image analysis for denoising homodyne FLIM data. The denoising routine is fast, improves the extraction of the fluorescence lifetime value(s) and increases the sensitivity and fluorescence lifetime resolving power of the FLIM instrument. The spatial resolution (especially the high spatial frequencies not related to noise) of the FLIM image is preserved, because the denoising routine does not blur or smooth the image. By eliminating the random noise known to be specific to photon noise and from the intensifier amplification, the fidelity of the spatial resolution is improved. The polar plot projection, a rapid FLIM analysis method, is used to demonstrate the effectiveness of the denoising routine with exemplary data from both physical and complex biological samples. We also suggest broader impacts of the image analysis for other fluorescence microscopy techniques (e.g. super-resolution imaging).

  14. Hyperspectral imaging for non-contact analysis of forensic traces.

    PubMed

    Edelman, G J; Gaston, E; van Leeuwen, T G; Cullen, P J; Aalders, M C G

    2012-11-30

    Hyperspectral imaging (HSI) integrates conventional imaging and spectroscopy, to obtain both spatial and spectral information from a specimen. This technique enables investigators to analyze the chemical composition of traces and simultaneously visualize their spatial distribution. HSI offers significant potential for the detection, visualization, identification and age estimation of forensic traces. The rapid, non-destructive and non-contact features of HSI mark its suitability as an analytical tool for forensic science. This paper provides an overview of the principles, instrumentation and analytical techniques involved in hyperspectral imaging. We describe recent advances in HSI technology motivating forensic science applications, e.g. the development of portable and fast image acquisition systems. Reported forensic science applications are reviewed. Challenges are addressed, such as the analysis of traces on backgrounds encountered in casework, concluded by a summary of possible future applications.

  15. SPR platform based on image acquisition for HER2 antigen detection

    NASA Astrophysics Data System (ADS)

    Monteiro, Johny P.; Predabon, Sheila M.; Bonafé, Elton G.; Martins, Alessandro F.; Brolo, Alexandre G.; Radovanovic, Eduardo; Girotto, Emerson M.

    2017-01-01

    HER2 antigen is a marker used for breast cancer diagnosis and prevention. Its determination has great importance since breast cancer is one of the most insidious types of cancer in women. HER2 antigen assessment in human serum is traditionally achieved by enzyme-linked immunosorbent assay (ELISA method), but it has some disadvantages, such as suppressing the thermodynamic-kinetic studies regarding the antibody-antigen interaction, and the use of labeled molecules that can promote false positive responses. Biosensors based on surface plasmon resonance (SPR) are sensitive optical techniques widely applied on bioassays. The plasmonic devices do not operate with labeled molecules, overcoming conventional immunoassay limitations, and enabling a direct detection of target analytes. In this way, a new SPR biosensor to assess HER2 antigen has been proposed, using nanohole arrays on a gold thin film by signal transduction of transmitted light measurements from array image acquisitions. These metallic nanostructures may couple the light directly on surface plasmons using a simple collinear arrangement. The proposed device reached an average sensitivity for refractive index (RI) variation on a metal surface of 4146 intensity units/RIU (RIU = RI units). The device feasibility on biomolecular assessment was evaluated. For this, 3 ng ml-1 known HER2 antigen concentration was efficiently flowed (using a microfluidic system) and detected from aqueous solutions. This outcome shows that the device may be a powerful apparatus for bioassays, particularly toward breast cancer diagnosis and prognosis.

  16. 3D imaging acquisition, modeling, and prototyping for facial defects reconstruction

    NASA Astrophysics Data System (ADS)

    Sansoni, Giovanna; Trebeschi, Marco; Cavagnini, Gianluca; Gastaldi, Giorgio

    2009-01-01

    A novel approach that combines optical three-dimensional imaging, reverse engineering (RE) and rapid prototyping (RP) for mold production in the prosthetic reconstruction of facial prostheses is presented. A commercial laser-stripe digitizer is used to perform the multiview acquisition of the patient's face; the point clouds are aligned and merged in order to obtain a polygonal model, which is then edited to sculpture the virtual prothesis. Two physical models of both the deformed face and the 'repaired' face are obtained: they differ only in the defect zone. Depending on the material used for the actual prosthesis, the two prototypes can be used either to directly cast the final prosthesis or to fabricate the positive wax pattern. Two case studies are presented, referring to prostetic reconstructions of an eye and of a nose. The results demonstrate the advantages over conventional techniques as well as the improvements with respect to known automated manufacturing techniques in the mold construction. The proposed method results into decreased patient's disconfort, reduced dependence on the anaplasthologist skill, increased repeatability and efficiency of the whole process.

  17. High-throughput data acquisition and processing for real-time x-ray imaging

    NASA Astrophysics Data System (ADS)

    Vogelgesang, Matthias; Rota, Lorenzo; Ardila Perez, Luis E.; Caselle, Michele; Chilingaryan, Suren; Kopmann, Andreas

    2016-10-01

    With ever-increasing data rates due to stronger light sources and better detectors, X-ray imaging experiments conducted at synchrotron beamlines face bandwidth and processing limitations that inhibit efficient workflows and prevent real-time operations. We propose an experiment platform comprised of programmable hardware and optimized software to lift these limitations and make beamline setups future-proof. The hardware consists of an FPGA-based data acquisition system with custom logic for data pre-processing and a PCIe data connection for transmission of currently up to 6.6 GB/s. Moreover, the accompanying firmware supports pushing data directly into GPU memory using AMD's DirectGMA technology without crossing system memory first. The GPUs are used to pre-process projection data and reconstruct final volumetric data with OpenCL faster than possible with CPUs alone. Besides, more efficient use of resources this enables a real-time preview of a reconstruction for early quality assessment of both experiment setup and the investigated sample. The entire system is designed in a modular way and allows swapping all components, e.g. replacing our custom FPGA camera with a commercial system but keep reconstructing data with GPUs. Moreover, every component is accessible using a low-level C library or using a high-level Python interface in order to integrate these components in any legacy environment.

  18. Acquisition of high-fidelity flyer characteristics using PDV and streak imaging

    NASA Astrophysics Data System (ADS)

    Olles, Joseph; Wixom, Ryan; Ball, J. Patrick; Kosiba, Graham

    2016-11-01

    Acquisition of experimental flight characteristics of electrically driven flyers (EDFs) is important in understanding the flyer's role in initiating detonator explosives. The velocity throughout a plastic flyer's flight was measured, as well as the magnitude and duration of the impulse while impacting an acrylic window. Despite the small size, thickness, and large accelerations of the EDFs, diagnostic techniques now have the temporal and spatially fidelity to measure validation-quality flyer characteristics. Using multipoint photonic Doppler velocimetry (PDV) in conjunction with streak imaging through a fiber array the velocity profile, bow shock (air cushion), time of impact, flyer shape at impact, and shock duration were measured. Shock physics simulations were then compared to this high fidelity data as a means of validating equations of state. Through the combination of experiments and simulations we can achieve a greater fundamental understanding of the energy transfer from the EDF to the energetic material prior to initiation. Sandia National Labs is a multi-program lab managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.

  19. iLAP: a workflow-driven software for experimental protocol development, data acquisition and analysis

    PubMed Central

    2009-01-01

    Background In recent years, the genome biology community has expended considerable effort to confront the challenges of managing heterogeneous data in a structured and organized way and developed laboratory information management systems (LIMS) for both raw and processed data. On the other hand, electronic notebooks were developed to record and manage scientific data, and facilitate data-sharing. Software which enables both, management of large datasets and digital recording of laboratory procedures would serve a real need in laboratories using medium and high-throughput techniques. Results We have developed iLAP (Laboratory data management, Analysis, and Protocol development), a workflow-driven information management system specifically designed to create and manage experimental protocols, and to analyze and share laboratory data. The system combines experimental protocol development, wizard-based data acquisition, and high-throughput data analysis into a single, integrated system. We demonstrate the power and the flexibility of the platform using a microscopy case study based on a combinatorial multiple fluorescence in situ hybridization (m-FISH) protocol and 3D-image reconstruction. iLAP is freely available under the open source license AGPL from http://genome.tugraz.at/iLAP/. Conclusion iLAP is a flexible and versatile information management system, which has the potential to close the gap between electronic notebooks and LIMS and can therefore be of great value for a broad scientific community. PMID:19941647

  20. Improving Image Quality and Reducing Drift Problems via Automated Data Acquisition and Averaging in Cs-corrected TEM

    SciTech Connect

    Voelkl, E; Jiang, B; Dai, Z R; Bradley, J P

    2008-08-29

    Image acquisition with a CCD camera is a single-press-button activity: after selecting exposure time and adjusting illumination, a button is pressed and the acquired image is perceived as the final, unmodified proof of what was seen in the microscope. Thus it is generally assumed that the image processing steps of e.g., 'dark-current correction' and 'gain normalization' do not alter the information content of the image, but rather eliminate unwanted artifacts. Image quality therefore is, among a long list of other parameters, defined by the dynamic range of the CCD camera as well as the maximum allowable exposure time depending on sample drift (ignoring sample damage). Despite the fact that most microscopists are satisfied with present, standard image quality we found that it is a relatively easy to improve on existing routines in at least two aspects: (1) Suppression of lateral image drift during acquisition by using significantly shorter exposure times with a plurality of exposures (3D-data set); and (2) Improvement in the Signal/Noise ratio by averaging over a given data set by exceeding the dynamic range of the camera.

  1. Big Data Analysis of Contractor Performance Information for Services Acquisition in DoD: A Proof of Concept

    DTIC Science & Technology

    2016-04-30

    Director, Acquisition Career Management , ASN(RD&A) Big Data Analysis of Contractor Performance Information for Services Acquisition in DoD: A Proof of...that the DoD lacks adequate data needed to inform its decision-making on services acquisition and contract management . The GAO has also stated that... data indicated that the current state of services acquisition management suffers from several deficiencies, including deficit billet and manning

  2. Cellular phone-based image acquisition and quantitative ratiometric method for detecting cocaine and benzoylecgonine for biological and forensic applications.

    PubMed

    Cadle, Brian A; Rasmus, Kristin C; Varela, Juan A; Leverich, Leah S; O'Neill, Casey E; Bachtell, Ryan K; Cooper, Donald C

    2010-01-01

    Here we describe the first report of using low-cost cellular or web-based digital cameras to image and quantify standardized rapid immunoassay strips as a new point-of-care diagnostic and forensics tool with health applications. Quantitative ratiometric pixel density analysis (QRPDA) is an automated method requiring end-users to utilize inexpensive (∼ $1 USD/each) immunotest strips, a commonly available web or mobile phone camera or scanner, and internet or cellular service. A model is described whereby a central computer server and freely available IMAGEJ image analysis software records and analyzes the incoming image data with time-stamp and geo-tag information and performs the QRPDA using custom JAVA based macros (http://www.neurocloud.org). To demonstrate QRPDA we developed a standardized method using rapid immunotest strips directed against cocaine and its major metabolite, benzoylecgonine. Images from standardized samples were acquired using several devices, including a mobile phone camera, web cam, and scanner. We performed image analysis of three brands of commercially available dye-conjugated anti-cocaine/benzoylecgonine (COC/BE) antibody test strips in response to three different series of cocaine concentrations ranging from 0.1 to 300 ng/ml and BE concentrations ranging from 0.003 to 0.1 ng/ml. This data was then used to create standard curves to allow quantification of COC/BE in biological samples. Across all devices, QRPDA quantification of COC and BE proved to be a sensitive, economical, and faster alternative to more costly methods, such as gas chromatography-mass spectrometry, tandem mass spectrometry, or high pressure liquid chromatography. The limit of detection was determined to be between 0.1 and 5 ng/ml. To simulate conditions in the field, QRPDA was found to be robust under a variety of image acquisition and testing conditions that varied temperature, lighting, resolution, magnification and concentrations of biological fluid in a sample. To

  3. Automated image analysis of uterine cervical images

    NASA Astrophysics Data System (ADS)

    Li, Wenjing; Gu, Jia; Ferris, Daron; Poirson, Allen

    2007-03-01

    Cervical Cancer is the second most common cancer among women worldwide and the leading cause of cancer mortality of women in developing countries. If detected early and treated adequately, cervical cancer can be virtually prevented. Cervical precursor lesions and invasive cancer exhibit certain morphologic features that can be identified during a visual inspection exam. Digital imaging technologies allow us to assist the physician with a Computer-Aided Diagnosis (CAD) system. In colposcopy, epithelium that turns white after application of acetic acid is called acetowhite epithelium. Acetowhite epithelium is one of the major diagnostic features observed in detecting cancer and pre-cancerous regions. Automatic extraction of acetowhite regions from cervical images has been a challenging task due to specular reflection, various illumination conditions, and most importantly, large intra-patient variation. This paper presents a multi-step acetowhite region detection system to analyze the acetowhite lesions in cervical images automatically. First, the system calibrates the color of the cervical images to be independent of screening devices. Second, the anatomy of the uterine cervix is analyzed in terms of cervix region, external os region, columnar region, and squamous region. Third, the squamous region is further analyzed and subregions based on three levels of acetowhite are identified. The extracted acetowhite regions are accompanied by color scores to indicate the different levels of acetowhite. The system has been evaluated by 40 human subjects' data and demonstrates high correlation with experts' annotations.

  4. Light field sensor and real-time panorama imaging multi-camera system and the design of data acquisition

    NASA Astrophysics Data System (ADS)

    Lu, Yu; Tao, Jiayuan; Wang, Keyi

    2014-09-01

    Advanced image sensor and powerful parallel data acquisition chip can be used to collect more detailed and comprehensive light field information. Using multiple single aperture and high resolution sensor record light field data, and processing the light field data real time, we can obtain wide field-of-view (FOV) and high resolution image. Wide FOV and high-resolution imaging has promising application in areas of navigation, surveillance and robotics. Qualityenhanced 3D rending, very high resolution depth map estimation, high dynamic-range and other applications we can obtained when we post-process these large light field data. The FOV and resolution are contradictions in traditional single aperture optic imaging system, and can't be solved very well. We have designed a multi-camera light field data acquisition system, and optimized each sensor's spatial location and relations. It can be used to wide FOV and high resolution real-time image. Using 5 megapixel CMOS sensors, and field programmable Gate Array (FPGA) acquisition light field data, paralleled processing and transmission to PC. A common clock signal is distributed to all of the cameras, and the precision of synchronization each camera achieved 40ns. Using 9 CMOSs build an initial system and obtained high resolution 360°×60° FOV image. It is intended to be flexible, modular and scalable, with much visibility and control over the cameras. In the system we used high speed dedicated camera interface CameraLink for system data transfer. The detail of the hardware architecture, its internal blocks, the algorithms, and the device calibration procedure are presented, along with imaging results.

  5. Pyroshock recommendations in proposed MIL-HDBK on 'Dynamic Data Acquisition and Analysis'

    NASA Technical Reports Server (NTRS)

    Piersol, Allan G.

    1991-01-01

    The pyroshock recommendations presented in a military handbook on Guidelines for Dynamic Data Acquisition and Analysis, which is being prepared by the Jet Propulsion Laboratory, are summarized. Numerous comments,including suggestions for modifications and additions to the handbook, are discussed. Particular attention is given to recommendations concerning measurement locations, transducers, signal conditioners, data recorders, data sampling, data editing, and data analysis.

  6. Pyroshock recommendations in proposed MIL-HDBK on 'Dynamic Data Acquisition and Analysis'

    NASA Astrophysics Data System (ADS)

    Piersol, Allan G.

    The pyroshock recommendations presented in a military handbook on Guidelines for Dynamic Data Acquisition and Analysis, which is being prepared by the Jet Propulsion Laboratory, are summarized. Numerous comments,including suggestions for modifications and additions to the handbook, are discussed. Particular attention is given to recommendations concerning measurement locations, transducers, signal conditioners, data recorders, data sampling, data editing, and data analysis.

  7. Space science technology: In-situ science. Sample Acquisition, Analysis, and Preservation Project summary

    NASA Technical Reports Server (NTRS)

    Aaron, Kim

    1991-01-01

    The Sample Acquisition, Analysis, and Preservation Project is summarized in outline and graphic form. The objective of the project is to develop component and system level technology to enable the unmanned collection, analysis and preservation of physical, chemical and mineralogical data from the surface of planetary bodies. Technology needs and challenges are identified and specific objectives are described.

  8. Visual Skills and Chinese Reading Acquisition: A Meta-Analysis of Correlation Evidence

    ERIC Educational Resources Information Center

    Yang, Ling-Yan; Guo, Jian-Peng; Richman, Lynn C.; Schmidt, Frank L.; Gerken, Kathryn C.; Ding, Yi

    2013-01-01

    This paper used meta-analysis to synthesize the relation between visual skills and Chinese reading acquisition based on the empirical results from 34 studies published from 1991 to 2011. We obtained 234 correlation coefficients from 64 independent samples, with a total of 5,395 participants. The meta-analysis revealed that visual skills as a…

  9. DDS-Suite - A Dynamic Data Acquisition, Processing, and Analysis System for Wind Tunnel Testing

    NASA Technical Reports Server (NTRS)

    Burnside, Jathan J.

    2012-01-01

    Wind Tunnels have optimized their steady-state data systems for acquisition and analysis and even implemented large dynamic-data acquisition systems, however development of near real-time processing and analysis tools for dynamic-data have lagged. DDS-Suite is a set of tools used to acquire, process, and analyze large amounts of dynamic data. Each phase of the testing process: acquisition, processing, and analysis are handled by separate components so that bottlenecks in one phase of the process do not affect the other, leading to a robust system. DDS-Suite is capable of acquiring 672 channels of dynamic data at rate of 275 MB / s. More than 300 channels of the system use 24-bit analog-to-digital cards and are capable of producing data with less than 0.01 of phase difference at 1 kHz. System architecture, design philosophy, and examples of use during NASA Constellation and Fundamental Aerodynamic tests are discussed.

  10. Breath-hold black blood quantitative T1rho imaging of liver using single shot fast spin echo acquisition

    PubMed Central

    Chan, Queenie; Wáng, Yì-Xiáng J.

    2016-01-01

    Background Liver fibrosis is a key feature in most chronic liver diseases. T1rho magnetic resonance imaging is a potentially important technique for noninvasive diagnosis, severity grading, and therapy monitoring of liver fibrosis. However, it remains challenging to perform robust T1rho quantification of liver on human subjects. One major reason is that the presence of rich blood signal in liver can cause artificially high T1rho measurement and makes T1rho quantification susceptible to motion. Methods A pulse sequence based on single shot fast/turbo spin echo (SSFSE/SSTSE) acquisition, with theoretical analysis and simulation based on the extended phase graph (EPG) algorithm, was presented for breath-hold single slice quantitative T1rho imaging of liver with suppression of blood signal. The pulse sequence was evaluated in human subjects at 3.0 T with 500 Hz spinlock frequency and time-of-spinlock (TSL) 0, 10, 30 and 50 ms. Results Human scan demonstrated that the entire T1rho data sets with four spinlock time can be acquired within a single breath-hold of 10 seconds with black blood effect. T1rho quantification with suppression of blood signal results in significantly reduced T1rho value of liver compared to the results without blood suppression. Conclusions A signal-to-noise ratio (SNR) efficient pulse sequence was reported for T1rho quantification of liver. The black blood effect, together with a short breath-hold, mitigates the risk of quantification errors as would occur in the conventional methods. PMID:27190769

  11. Analysis of Phosphate Acquisition Efficiency in Different Arabidopsis Accessions

    PubMed Central

    Narang, Ram A.; Bruene, Asja; Altmann, Thomas

    2000-01-01

    The morphological and physiological characteristics of Arabidopsis accessions differing in their phosphate acquisition efficiencies (PAEs) when grown on a sparingly soluble phosphate source (hydroxylapatite) were analyzed. A set of 36 accessions was subjected to an initial PAE evaluation following cultivation on synthetic, agarose-solidified media containing potassium phosphate (soluble) or hydroxylapatite (sparingly soluble). From the five most divergent accessions identified in this way, C24, Co, and Cal exhibited high PAEs, whereas Col-0 and Te exhibited low PAEs. These five accessions were analyzed in detail. Significant differences were found in root morphology, phosphate uptake kinetics, organic acid release, rhizosphere acidification, and the ability of roots to penetrate substrates. Long root hairs at high densities, high uptake per unit root length, and high substrate penetration ability in the efficient accessions C24 and Co mediate their high PAEs. The third accession with high PAE, Cal, exhibits a high shoot-to-root ratio, long roots with long root hairs, and rhizosphere acidification. These results are consistent with previous observations and highlight the suitability of using Arabidopsis accessions to identify and isolate genes determining the PAE in plants. PMID:11115894

  12. Automated Microarray Image Analysis Toolbox for MATLAB

    SciTech Connect

    White, Amanda M.; Daly, Don S.; Willse, Alan R.; Protic, Miroslava; Chandler, Darrell P.

    2005-09-01

    The Automated Microarray Image Analysis (AMIA) Toolbox for MATLAB is a flexible, open-source microarray image analysis tool that allows the user to customize analysis of sets of microarray images. This tool provides several methods of identifying and quantify spot statistics, as well as extensive diagnostic statistics and images to identify poor data quality or processing. The open nature of this software allows researchers to understand the algorithms used to provide intensity estimates and to modify them easily if desired.

  13. Multispectral Image Analysis of Hurricane Gilbert

    DTIC Science & Technology

    1989-05-19

    Classification) Multispectral Image Analysis of Hurrican Gilbert (unclassified) 12. PERSONAL AUTHOR(S) Kleespies, Thomas J. (GL/LYS) 13a. TYPE OF REPORT...cloud top height. component, of tle image in the red channel, and similarly for the green and blue channels. Multispectral Muti.pectral image analysis can...However, there seems to be few references to the human range of vision, the selection as to which mllti.pp.tral image analysis of scenes or

  14. Pyroshock Data Acquisition and Analysis for U/RGM-109D Payload Cover Ejection Tests

    DTIC Science & Technology

    1988-09-01

    NWC TP 692" N Pyroshock Data Acquisition and Analysis for U/RGM-109D Payload Cover Ejection Tests by Allan G. Piersol Astron Research and Engineering...distribution is unlimited. 90 ’ ’W 051 Naval Weapons Center FOREWORD This is a final report on the U/RGM- 109 payload cover ejection pyroshock data...5460 ELEMENT NO. NO. NO PMA- ACCESSION NO I I2805A-88-01 i TITLE (Include Security Classification) PYROSHOCK DATA ACQUISITION AND ANALYSIS FOR U/RGM

  15. Evaluation of stereoscopic 3D displays for image analysis tasks

    NASA Astrophysics Data System (ADS)

    Peinsipp-Byma, E.; Rehfeld, N.; Eck, R.

    2009-02-01

    In many application domains the analysis of aerial or satellite images plays an important role. The use of stereoscopic display technologies can enhance the image analyst's ability to detect or to identify certain objects of interest, which results in a higher performance. Changing image acquisition from analog to digital techniques entailed the change of stereoscopic visualisation techniques. Recently different kinds of digital stereoscopic display techniques with affordable prices have appeared on the market. At Fraunhofer IITB usability tests were carried out to find out (1) with which kind of these commercially available stereoscopic display techniques image analysts achieve the best performance and (2) which of these techniques achieve a high acceptance. First, image analysts were interviewed to define typical image analysis tasks which were expected to be solved with a higher performance using stereoscopic display techniques. Next, observer experiments were carried out whereby image analysts had to solve defined tasks with different visualization techniques. Based on the experimental results (performance parameters and qualitative subjective evaluations of the used display techniques) two of the examined stereoscopic display technologies were found to be very good and appropriate.

  16. Measurements and analysis of active/passive multispectral imaging

    NASA Astrophysics Data System (ADS)

    Grönwall, Christina; Hamoir, Dominique; Steinvall, Ove; Larsson, Hâkan; Amselem, Elias; Lutzmann, Peter; Repasi, Endre; Göhler, Benjamin; Barbé, Stéphane; Vaudelin, Olivier; Fracès, Michel; Tanguy, Bernard; Thouin, Emmanuelle

    2013-10-01

    This paper describes a data collection on passive and active imaging and the preliminary analysis. It is part of an ongoing work on active and passive imaging for target identification using different wavelength bands. We focus on data collection at NIR-SWIR wavelengths but we also include the visible and the thermal region. Active imaging in NIRSWIR will support the passive imaging by eliminating shadows during day-time and allow night operation. Among the applications that are most likely for active multispectral imaging, we focus on long range human target identification. We also study the combination of active and passive sensing. The target scenarios of interest include persons carrying different objects and their associated activities. We investigated laser imaging for target detection and classification up to 1 km assuming that another cueing sensor - passive EO and/or radar - is available for target acquisition and detection. Broadband or multispectral operation will reduce the effects of target speckle and atmospheric turbulence. Longer wavelengths will improve performance in low visibility conditions due to haze, clouds and fog. We are currently performing indoor and outdoor tests to further investigate the target/background phenomena that are emphasized in these wavelengths. We also investigate how these effects can be used for target identification and image fusion. Performed field tests and the results of preliminary data analysis are reported.

  17. An Analysis of the Performance-Based Service Acquisition (PBSA) and Its Applicability to Hellenic Navy Service Acquisition Activities

    DTIC Science & Technology

    2012-12-01

    the time for reviewing instruction, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the...Navy service acquisition polices. This MBA Report includes a literature review and background information about PBSA, current PBSA implementation...to Hellenic Navy service acquisition polices. This MBA Report includes a literature review and background information about PBSA, current PBSA

  18. Acquisition of Robotized Metallographic Equipment with Automated Laue Pattern Analysis

    DTIC Science & Technology

    2008-10-23

    precision polishing system $ 20,577.86 • Microfocus x - ray source with focusing optics and associated metrology $ 169,331.00 • Machine shop charges...lens coupled Gemstar-2 intensified x - ray Laue imaging camera $ 86,200.00 • Nikon flat top stage for Laue diffractometer $ 15,313.23 • Multiprep...final design for the x - ray Laue diffrac- tomcter system could begin. Components for this system, which was designed in collaboration with Dr. Daniel

  19. On the brain structure heterogeneity of autism: Parsing out acquisition site effects with significance-weighted principal component analysis.

    PubMed

    Martinez-Murcia, Francisco Jesús; Lai, Meng-Chuan; Górriz, Juan Manuel; Ramírez, Javier; Young, Adam M H; Deoni, Sean C L; Ecker, Christine; Lombardo, Michael V; Baron-Cohen, Simon; Murphy, Declan G M; Bullmore, Edward T; Suckling, John

    2017-03-01

    Neuroimaging studies have reported structural and physiological differences that could help understand the causes and development of Autism Spectrum Disorder (ASD). Many of them rely on multisite designs, with the recruitment of larger samples increasing statistical power. However, recent large-scale studies have put some findings into question, considering the results to be strongly dependent on the database used, and demonstrating the substantial heterogeneity within this clinically defined category. One major source of variance may be the acquisition of the data in multiple centres. In this work we analysed the differences found in the multisite, multi-modal neuroimaging database from the UK Medical Research Council Autism Imaging Multicentre Study (MRC AIMS) in terms of both diagnosis and acquisition sites. Since the dissimilarities between sites were higher than between diagnostic groups, we developed a technique called Significance Weighted Principal Component Analysis (SWPCA) to reduce the undesired intensity variance due to acquisition site and to increase the statistical power in detecting group differences. After eliminating site-related variance, statistically significant group differences were found, including Broca's area and the temporo-parietal junction. However, discriminative power was not sufficient to classify diagnostic groups, yielding accuracies results close to random. Our work supports recent claims that ASD is a highly heterogeneous condition that is difficult to globally characterize by neuroimaging, and therefore different (and more homogenous) subgroups should be defined to obtain a deeper understanding of ASD. Hum Brain Mapp 38:1208-1223, 2017. © 2016 Wiley Periodicals, Inc.

  20. On the brain structure heterogeneity of autism: Parsing out acquisition site effects with significance‐weighted principal component analysis

    PubMed Central

    Martinez‐Murcia, Francisco Jesús; Lai, Meng‐Chuan; Ramírez, Javier; Young, Adam M. H.; Deoni, Sean C. L.; Ecker, Christine; Lombardo, Michael V.; Baron‐Cohen, Simon; Murphy, Declan G. M.; Bullmore, Edward T.; Suckling, John

    2016-01-01

    Abstract Neuroimaging studies have reported structural and physiological differences that could help understand the causes and development of Autism Spectrum Disorder (ASD). Many of them rely on multisite designs, with the recruitment of larger samples increasing statistical power. However, recent large‐scale studies have put some findings into question, considering the results to be strongly dependent on the database used, and demonstrating the substantial heterogeneity within this clinically defined category. One major source of variance may be the acquisition of the data in multiple centres. In this work we analysed the differences found in the multisite, multi‐modal neuroimaging database from the UK Medical Research Council Autism Imaging Multicentre Study (MRC AIMS) in terms of both diagnosis and acquisition sites. Since the dissimilarities between sites were higher than between diagnostic groups, we developed a technique called Significance Weighted Principal Component Analysis (SWPCA) to reduce the undesired intensity variance due to acquisition site and to increase the statistical power in detecting group differences. After eliminating site‐related variance, statistically significant group differences were found, including Broca's area and the temporo‐parietal junction. However, discriminative power was not sufficient to classify diagnostic groups, yielding accuracies results close to random. Our work supports recent claims that ASD is a highly heterogeneous condition that is difficult to globally characterize by neuroimaging, and therefore different (and more homogenous) subgroups should be defined to obtain a deeper understanding of ASD. Hum Brain Mapp 38:1208–1223, 2017. © 2016 Wiley Periodicals, Inc. PMID:27774713

  1. In-Depth Analysis of Computer Memory Acquisition Software for Forensic Purposes.

    PubMed

    McDown, Robert J; Varol, Cihan; Carvajal, Leonardo; Chen, Lei

    2016-01-01

    The comparison studies on random access memory (RAM) acquisition tools are either limited in metrics or the selected tools were designed to be executed in older operating systems. Therefore, this study evaluates widely used seven shareware or freeware/open source RAM acquisition forensic tools that are compatible to work with the latest 64-bit Windows operating systems. These tools' user interface capabilities, platform limitations, reporting capabilities, total execution time, shared and proprietary DLLs, modified registry keys, and invoked files during processing were compared. We observed that Windows Memory Reader and Belkasoft's Live Ram Capturer leaves the least fingerprints in memory when loaded. On the other hand, ProDiscover and FTK Imager perform poor in memory usage, processing time, DLL usage, and not-wanted artifacts introduced to the system. While Belkasoft's Live Ram Capturer is the fastest to obtain an image of the memory, Pro Discover takes the longest time to do the same job.

  2. Image gathering, coding, and processing: End-to-end optimization for efficient and robust acquisition of visual information

    NASA Technical Reports Server (NTRS)

    Huck, Friedrich O.; Fales, Carl L.

    1990-01-01

    Researchers are concerned with the end-to-end performance of image gathering, coding, and processing. The applications range from high-resolution television to vision-based robotics, wherever the resolution, efficiency and robustness of visual information acquisition and processing are critical. For the presentation at this workshop, it is convenient to divide research activities into the following two overlapping areas: The first is the development of focal-plane processing techniques and technology to effectively combine image gathering with coding, with an emphasis on low-level vision processing akin to the retinal processing in human vision. The approach includes the familiar Laplacian pyramid, the new intensity-dependent spatial summation, and parallel sensing/processing networks. Three-dimensional image gathering is attained by combining laser ranging with sensor-array imaging. The second is the rigorous extension of information theory and optimal filtering to visual information acquisition and processing. The goal is to provide a comprehensive methodology for quantitatively assessing the end-to-end performance of image gathering, coding, and processing.

  3. Motofit - integrating neutron reflectometry acquisition, reduction and analysis into one, easy to use, package

    NASA Astrophysics Data System (ADS)

    Nelson, Andrew

    2010-11-01

    The efficient use of complex neutron scattering instruments is often hindered by the complex nature of their operating software. This complexity exists at each experimental step: data acquisition, reduction and analysis, with each step being as important as the previous. For example, whilst command line interfaces are powerful at automated acquisition they often reduce accessibility by novice users and sometimes reduce the efficiency for advanced users. One solution to this is the development of a graphical user interface which allows the user to operate the instrument by a simple and intuitive "push button" approach. This approach was taken by the Motofit software package for analysis of multiple contrast reflectometry data. Here we describe the extension of this package to cover the data acquisition and reduction steps for the Platypus time-of-flight neutron reflectometer. Consequently, the complete operation of an instrument is integrated into a single, easy to use, program, leading to efficient instrument usage.

  4. Split-screen display system and standardized methods for ultrasound image acquisition and multi-frame data processing

    NASA Technical Reports Server (NTRS)

    Selzer, Robert H. (Inventor); Hodis, Howard N. (Inventor)

    2011-01-01

    A standardized acquisition methodology assists operators to accurately replicate high resolution B-mode ultrasound images obtained over several spaced-apart examinations utilizing a split-screen display in which the arterial ultrasound image from an earlier examination is displayed on one side of the screen while a real-time "live" ultrasound image from a current examination is displayed next to the earlier image on the opposite side of the screen. By viewing both images, whether simultaneously or alternately, while manually adjusting the ultrasound transducer, an operator is able to bring into view the real-time image that best matches a selected image from the earlier ultrasound examination. Utilizing this methodology, dynamic material properties of arterial structures, such as IMT and diameter, are measured in a standard region over successive image frames. Each frame of the sequence has its echo edge boundaries automatically determined by using the immediately prior frame's true echo edge coordinates as initial boundary conditions. Computerized echo edge recognition and tracking over multiple successive image frames enhances measurement of arterial diameter and IMT and allows for improved vascular dimension measurements, including vascular stiffness and IMT determinations.

  5. Quantifying the impact of respiratory-gated 4D CT acquisition on thoracic image quality: A digital phantom study

    SciTech Connect

    Bernatowicz, K. Knopf, A.; Lomax, A.; Keall, P.; Kipritidis, J.; Mishra, P.

    2015-01-15

    Purpose: Prospective respiratory-gated 4D CT has been shown to reduce tumor image artifacts by up to 50% compared to conventional 4D CT. However, to date no studies have quantified the impact of gated 4D CT on normal lung tissue imaging, which is important in performing dose calculations based on accurate estimates of lung volume and structure. To determine the impact of gated 4D CT on thoracic image quality, the authors developed a novel simulation framework incorporating a realistic deformable digital phantom driven by patient tumor motion patterns. Based on this framework, the authors test the hypothesis that respiratory-gated 4D CT can significantly reduce lung imaging artifacts. Methods: Our simulation framework synchronizes the 4D extended cardiac torso (XCAT) phantom with tumor motion data in a quasi real-time fashion, allowing simulation of three 4D CT acquisition modes featuring different levels of respiratory feedback: (i) “conventional” 4D CT that uses a constant imaging and couch-shift frequency, (ii) “beam paused” 4D CT that interrupts imaging to avoid oversampling at a given couch position and respiratory phase, and (iii) “respiratory-gated” 4D CT that triggers acquisition only when the respiratory motion fulfills phase-specific displacement gating windows based on prescan breathing data. Our framework generates a set of ground truth comparators, representing the average XCAT anatomy during beam-on for each of ten respiratory phase bins. Based on this framework, the authors simulated conventional, beam-paused, and respiratory-gated 4D CT images using tumor motion patterns from seven lung cancer patients across 13 treatment fractions, with a simulated 5.5 cm{sup 3} spherical lesion. Normal lung tissue image quality was quantified by comparing simulated and ground truth images in terms of overall mean square error (MSE) intensity difference, threshold-based lung volume error, and fractional false positive/false negative rates. Results

  6. Research and design of portable photoelectric rotary table data-acquisition and analysis system

    NASA Astrophysics Data System (ADS)

    Yang, Dawei; Yang, Xiufang; Han, Junfeng; Yan, Xiaoxu

    2015-02-01

    Photoelectric rotary table as the main test tracking measurement platform, widely use in shooting range and aerospace fields. In the range of photoelectric tracking measurement system, in order to meet the photoelectric testing instruments and equipment of laboratory and field application demand, research and design the portable photoelectric rotary table data acquisition and analysis system, and introduces the FPGA device based on Xilinx company Virtex-4 series and its peripheral module of the system hardware design, and the software design of host computer in VC++ 6.0 programming platform and MFC package based on class libraries. The data acquisition and analysis system for data acquisition, display and storage, commission control, analysis, laboratory wave playback, transmission and fault diagnosis, and other functions into an organic whole, has the advantages of small volume, can be embedded, high speed, portable, simple operation, etc. By photoelectric tracking turntable as experimental object, carries on the system software and hardware alignment, the experimental results show that the system can realize the data acquisition, analysis and processing of photoelectric tracking equipment and control of turntable debugging good, and measurement results are accurate, reliable and good maintainability and extensibility. The research design for advancing the photoelectric tracking measurement equipment debugging for diagnosis and condition monitoring and fault analysis as well as the standardization and normalization of the interface and improve the maintainability of equipment is of great significance, and has certain innovative and practical value.

  7. Computerized image analysis as a tool to quantify infiltrating leukocytes: a comparison between high- and low-magnification images.

    PubMed

    Johansson, A C; Visse, E; Widegren, B; Sjögren, H O; Siesjö, P

    2001-09-01

    The purpose of the present study was to establish a rapid and reproducible method for quantification of tissue-infiltrating leukocytes using computerized image analysis. To achieve this, the staining procedure, the image acquisition, and the image analysis method were optimized. Because of the adaptive features of the human eye, computerized image analysis is more sensitive to variations in staining compared with manual image analysis. To minimize variations in staining, an automated immunostainer was used. With a digital scanner camera, low-magnification images could be sampled at high resolution, thus making it possible to analyze larger tissue sections. Image analysis was performed by color thresholding of the digital images based on values of hue, saturation, and intensity color mode, which we consider superior to the red, green, and blue color mode for analysis of most histological stains. To evaluate the method, we compared computerized analysis of images with a x100 or a x12.5 magnification to assess leukocytes infiltrating rat brain tumors after peripheral immunizations with tumor cells genetically modified to express rat interferon-gamma (IFN-gamma) or medium controls. The results generated by both methods correlated well and did not show any significant differences. The method allows efficient and reproducible processing of large tissue sections that is less time-consuming than conventional methods and can be performed with standard equipment and software.(J Histochem Cytochem 49:1073-1079, 2001)

  8. In vivo testing of a prototype system providing simultaneous white light and near infrared autofluorescence image acquisition for detection of bladder cancer.

    PubMed

    Jacobson, Michael C; deVere White, Ralph; Demos, Stavros G

    2012-03-01

    A prototype instrument developed to provide simultaneously ordinary visual endoscopy together with near infrared (NIR) autofluorescence imaging via parallel image acquisition is demonstrated. The two images are recorded concurrently and the instrument interfaces with any ordinary endoscope. Preliminary results of a pilot study focused on imaging of bladder tumors in vivo using this instrumentation are presented. The experimental results demonstrate the capabilities of this instrumentation design, imaging methodology, and define the current limitation for further development of the system.

  9. Handbook for Dynamic Data Acquisition and Analysis - IES Recommended Practices 012.1

    NASA Technical Reports Server (NTRS)

    Himelblau, H.; Wise, J. W.; Piersol, A. G.; Grundvig, M. R.

    1994-01-01

    The original motivation for this Handbook came from personnel of The Aerospace Corporation (TAC), who are technical advisors to the Air Force Space and Missile Systems Center. Over the preceding two decades, TAC has observed a seemingly endless series of errors and anomalies in the acquisition and analysis of structural dynamic and aeroacoustic data.

  10. Principles and clinical applications of image analysis.

    PubMed

    Kisner, H J

    1988-12-01

    Image processing has traveled to the lunar surface and back, finding its way into the clinical laboratory. Advances in digital computers have improved the technology of image analysis, resulting in a wide variety of medical applications. Offering improvements in turnaround time, standardized systems, increased precision, and walkaway automation, digital image analysis has likely found a permanent home as a diagnostic aid in the interpretation of microscopic as well as macroscopic laboratory images.

  11. Differentiating semantic categories during the acquisition of novel words: correspondence analysis applied to event-related potentials.

    PubMed

    Fargier, Raphaël; Ploux, Sabine; Cheylus, Anne; Reboul, Anne; Paulignan, Yves; Nazir, Tatjana A

    2014-11-01

    Growing evidence suggests that semantic knowledge is represented in distributed neural networks that include modality-specific structures. Here, we examined the processes underlying the acquisition of words from different semantic categories to determine whether the emergence of visual- and action-based categories could be tracked back to their acquisition. For this, we applied correspondence analysis (CA) to ERPs recorded at various moments during acquisition. CA is a multivariate statistical technique typically used to reveal distance relationships between words of a corpus. Applied to ERPs, it allows isolating factors that best explain variations in the data across time and electrodes. Participants were asked to learn new action and visual words by associating novel pseudowords with the execution of hand movements or the observation of visual images. Words were probed before and after training on two consecutive days. To capture processes that unfold during lexical access, CA was applied on the 100-400 msec post-word onset interval. CA isolated two factors that organized the data as a function of test sessions and word categories. Conventional ERP analyses further revealed a category-specific increase in the negativity of the ERPs to action and visual words at the frontal and occipital electrodes, respectively. The distinct neural processes underlying action and visual words can thus be tracked back to the acquisition of word-referent relationships and may have its origin in association learning. Given current evidence for the flexibility of language-induced sensory-motor activity, we argue that these associative links may serve functions beyond word understanding, that is, the elaboration of situation models.

  12. FFDM image quality assessment using computerized image texture analysis

    NASA Astrophysics Data System (ADS)

    Berger, Rachelle; Carton, Ann-Katherine; Maidment, Andrew D. A.; Kontos, Despina

    2010-04-01

    Quantitative measures of image quality (IQ) are routinely obtained during the evaluation of imaging systems. These measures, however, do not necessarily correlate with the IQ of the actual clinical images, which can also be affected by factors such as patient positioning. No quantitative method currently exists to evaluate clinical IQ. Therefore, we investigated the potential of using computerized image texture analysis to quantitatively assess IQ. Our hypothesis is that image texture features can be used to assess IQ as a measure of the image signal-to-noise ratio (SNR). To test feasibility, the "Rachel" anthropomorphic breast phantom (Model 169, Gammex RMI) was imaged with a Senographe 2000D FFDM system (GE Healthcare) using 220 unique exposure settings (target/filter, kVs, and mAs combinations). The mAs were varied from 10%-300% of that required for an average glandular dose (AGD) of 1.8 mGy. A 2.5cm2 retroareolar region of interest (ROI) was segmented from each image. The SNR was computed from the ROIs segmented from images linear with dose (i.e., raw images) after flat-field and off-set correction. Image texture features of skewness, coarseness, contrast, energy, homogeneity, and fractal dimension were computed from the Premium ViewTM postprocessed image ROIs. Multiple linear regression demonstrated a strong association between the computed image texture features and SNR (R2=0.92, p<=0.001). When including kV, target and filter as additional predictor variables, a stronger association with SNR was observed (R2=0.95, p<=0.001). The strong associations indicate that computerized image texture analysis can be used to measure image SNR and potentially aid in automating IQ assessment as a component of the clinical workflow. Further work is underway to validate our findings in larger clinical datasets.

  13. Mediman: Object oriented programming approach for medical image analysis

    SciTech Connect

    Coppens, A.; Sibomana, M.; Bol, A.; Michel, C. . Positron Tomography Lab.)

    1993-08-01

    Mediman is a new image analysis package which has been developed to analyze quantitatively Positron Emission Tomography (PET) data. It is object-oriented, written in C++ and its user interface is based on InterViews on top of which new classes have been added. Mediman accesses data using external data representation or import/export mechanism which avoids data duplication. Multimodality studies are organized in a simple database which includes images, headers, color tables, lists and objects of interest (OOI's) and history files. Stored color table parameters allow to focus directly on the interesting portion of the dynamic range. Lists allow to organize the study according to modality, acquisition protocol, time and spatial properties. OOI's (points, lines and regions) are stored in absolute 3-D coordinates allowing correlation with other co-registered imaging modalities such as MRI or SPECT. OOI's have visualization properties and are organized into groups. Quantitative ROI analysis of anatomic images consists of position, distance, volume calculation on selected OOI's. An image calculator is connected to mediman. Quantitation of metabolic images is performed via profiles, sectorization, time activity curves and kinetic modeling. Mediman is menu and mouse driven, macro-commands can be registered and replayed. Its interface is customizable through a configuration file. The benefit of the object-oriented approach are discussed from a development point of view.

  14. Development of an acquisition protocol and a segmentation algortihm for wounds of cutaneous Leishmaniasis in digital images

    NASA Astrophysics Data System (ADS)

    Diaz, Kristians; Castañeda, Benjamín; Miranda, César; Lavarello, Roberto; Llanos, Alejandro

    2010-03-01

    We developed a protocol for the acquisition of digital images and an algorithm for a color-based automatic segmentation of cutaneous lesions of Leishmaniasis. The protocol for image acquisition provides control over the working environment to manipulate brightness, lighting and undesirable shadows on the injury using indirect lighting. Also, this protocol was used to accurately calculate the area of the lesion expressed in mm2 even in curved surfaces by combining the information from two consecutive images. Different color spaces were analyzed and compared using ROC curves in order to determine the color layer with the highest contrast between the background and the wound. The proposed algorithm is composed of three stages: (1) Location of the wound determined by threshold and mathematical morphology techniques to the H layer of the HSV color space, (2) Determination of the boundaries of the wound by analyzing the color characteristics in the YIQ space based on masks (for the wound and the background) estimated from the first stage, and (3) Refinement of the calculations obtained on the previous stages by using the discrete dynamic contours algorithm. The segmented regions obtained with the algorithm were compared with manual segmentations made by a medical specialist. Broadly speaking, our results support that color provides useful information during segmentation and measurement of wounds of cutaneous Leishmaniasis. Results from ten images showed 99% specificity, 89% sensitivity, and 98% accuracy.

  15. Nonintrusive iris image acquisition system based on a pan-tilt-zoom camera and light stripe projection

    NASA Astrophysics Data System (ADS)

    Yoon, Soweon; Jung, Ho Gi; Park, Kang Ryoung; Kim, Jaihie

    2009-03-01

    Although iris recognition is one of the most accurate biometric technologies, it has not yet been widely used in practical applications. This is mainly due to user inconvenience during the image acquisition phase. Specifically, users try to adjust their eye position within small capture volume at a close distance from the system. To overcome these problems, we propose a novel iris image acquisition system that provides users with unconstrained environments: a large operating range, enabling movement from standing posture, and capturing good-quality iris images in an acceptable time. The proposed system has the following three contributions compared with previous works: (1) the capture volume is significantly increased by using a pan-tilt-zoom (PTZ) camera guided by a light stripe projection, (2) the iris location in the large capture volume is found fast due to 1-D vertical face searching from the user's horizontal position obtained by the light stripe projection, and (3) zooming and focusing on the user's irises at a distance are accurate and fast using the estimated 3-D position of a face by the light stripe projection and the PTZ camera. Experimental results show that the proposed system can capture good-quality iris images in 2.479 s on average at a distance of 1.5 to 3 m, while allowing a limited amount of movement by the user.

  16. Acquisition and analysis of primate physiologic data for the Space Shuttle

    NASA Technical Reports Server (NTRS)

    Eberhart, Russell C.; Hogrefe, Arthur F.; Radford, Wade E.; Sanders, Kermit H.; Dobbins, Roy W.

    1988-01-01

    This paper describes the design and prototypes of the Physiologic Acquisition and Telemetry System (PATS), which is a multichannel system, designed for large primates, for the data acquisition, telemetry, storage, and analysis of physiological data. PATS is expected to acquire data from units implanted in the abdominal cavities of rhesus monkeys that will be flown aboard the Spacelab. The system will telemeter both stored and real-time internal physiologic measurements to an external Flight Support Station (FSS) computer system. The implanted Data Acquition and Telemetry Subsystem subunit will be externally activated, controlled and reprogrammed from the FSS.

  17. Acquisition and analysis of primate physiologic data for the Space Shuttle

    NASA Astrophysics Data System (ADS)

    Eberhart, Russell C.; Hogrefe, Arthur F.; Radford, Wade E.; Sanders, Kermit H.; Dobbins, Roy W.

    1988-03-01

    This paper describes the design and prototypes of the Physiologic Acquisition and Telemetry System (PATS), which is a multichannel system, designed for large primates, for the data acquisition, telemetry, storage, and analysis of physiological data. PATS is expected to acquire data from units implanted in the abdominal cavities of rhesus monkeys that will be flown aboard the Spacelab. The system will telemeter both stored and real-time internal physiologic measurements to an external Flight Support Station (FSS) computer system. The implanted Data Acquition and Telemetry Subsystem subunit will be externally activated, controlled and reprogrammed from the FSS.

  18. Impact of the Timing of a SAR Image Acquisition on the Calibration of a Flood Inundation Model

    NASA Technical Reports Server (NTRS)

    Gobeyn, Sacha; Van Wesemael, Alexandra; Neal, Jeffrey; Lievens, Hans; Van Eerdenbrugh, Katrien; De Vleeschouwer, Niels; Vernieuwe, Hilde; Schumann, Guy J.-P.; Di Baldassarre, Giuliano; De Baets, Bernard; Bates, Paul D.; Verhoest, Niko E. C.

    2016-01-01

    Synthetic Aperture Radar (SAR) data have proven to be a very useful source of information for the calibration of flood inundation models. Previous studies have focused on assigning uncertainties to SAR images in order to improve flood forecast systems (e.g. Giustarini et al. (2015) and Stephens et al. (2012)). This paper investigates whether the timing of a SAR acquisition of a flood has an important impact on the calibration of a flood inundation model. As no suitable time series of SAR data exists, we generate a sequence of consistent SAR images through the use of a synthetic framework. This framework uses two available ERS-2 SAR images of the study area, one taken during the flood event of interest, the second taken during a dry reference period. The obtained synthetic observations at different points in time during the flood event are used to calibrate the flood inundation model. The results of this study indicate that the uncertainty of the roughness parameters is lower when the model is calibrated with an image taken before rather than during or after the flood peak. The results also show that the error on the modeled extent is much lower when the model is calibrated with a pre-flood peak image than when calibrated with a near-flood peak or a post-flood peak image. It is concluded that the timing of the SAR image acquisition of the flood has a clear impact on the model calibration and consequently on the precision of the predicted flood extent.

  19. An adaptive undersampling scheme of wavelet-encoded parallel MR imaging for more efficient MR data acquisition

    NASA Astrophysics Data System (ADS)

    Xie, Hua; Bosshard, John C.; Hill, Jason E.; Wright, Steven M.; Mitra, Sunanda

    2016-03-01

    Magnetic Resonance Imaging (MRI) offers noninvasive high resolution, high contrast cross-sectional anatomic images through the body. The data of the conventional MRI is collected in spatial frequency (Fourier) domain, also known as kspace. Because there is still a great need to improve temporal resolution of MRI, Compressed Sensing (CS) in MR imaging is proposed to exploit the sparsity of MR images showing great potential to reduce the scan time significantly, however, it poses its own unique problems. This paper revisits wavelet-encoded MR imaging which replaces phase encoding in conventional MRI data acquisition with wavelet encoding by applying wavelet-shaped spatially selective radiofrequency (RF) excitation, and keeps the readout direction as frequency encoding. The practicality of wavelet encoded MRI by itself is limited due to the SNR penalties and poor time resolution compared to conventional Fourier-based MRI. To compensate for those disadvantages, this paper first introduces an undersampling scheme named significance map for sparse wavelet-encoded k-space to speed up data acquisition as well as allowing for various adaptive imaging strategies. The proposed adaptive wavelet-encoded undersampling scheme does not require prior knowledge of the subject to be scanned. Multiband (MB) parallel imaging is also incorporated with wavelet-encoded MRI by exciting multiple regions simultaneously for further reduction in scan time desirable for medical applications. The simulation and experimental results are presented showing the feasibility of the proposed approach in further reduction of the redundancy of the wavelet k-space data while maintaining relatively high quality.

  20. Impact of the timing of a SAR image acquisition on the calibration of a flood inundation model

    NASA Astrophysics Data System (ADS)

    Gobeyn, Sacha; Van Wesemael, Alexandra; Neal, Jeffrey; Lievens, Hans; Eerdenbrugh, Katrien Van; De Vleeschouwer, Niels; Vernieuwe, Hilde; Schumann, Guy J.-P.; Di Baldassarre, Giuliano; Baets, Bernard De; Bates, Paul D.; Verhoest, Niko E. C.

    2017-02-01

    Synthetic Aperture Radar (SAR) data have proven to be a very useful source of information for the calibration of flood inundation models. Previous studies have focused on assigning uncertainties to SAR images in order to improve flood forecast systems (e.g. Giustarini et al. (2015) and Stephens et al. (2012)). This paper investigates whether the timing of a SAR acquisition of a flood has an important impact on the calibration of a flood inundation model. As no suitable time series of SAR data exists, we generate a sequence of consistent SAR images through the use of a synthetic framework. This framework uses two available ERS-2 SAR images of the study area, one taken during the flood event of interest, the second taken during a dry reference period. The obtained synthetic observations at different points in time during the flood event are used to calibrate the flood inundation model. The results of this study indicate that the uncertainty of the roughness parameters is lower when the model is calibrated with an image taken before rather than during or after the flood peak. The results also show that the error on the modelled extent is much lower when the model is calibrated with a pre-flood peak image than when calibrated with a near-flood peak or a post-flood peak image. It is concluded that the timing of the SAR image acquisition of the flood has a clear impact on the model calibration and consequently on the precision of the predicted flood extent.

  1. A Stylistic Approach to Foreign Language Acquisition and Literary Analysis.

    ERIC Educational Resources Information Center

    Berg, William J.; Martin-Berg, Laurey K.

    This paper discusses an approach to teaching third college year "bridge" courses, showing that students in a course that focuses on language and culture as well as students in an introductory course on literary analysis can benefit from using a stylistic approach to literacy texts to understand both form and content. The paper suggests that a…

  2. Image analysis: a consumer's guide.

    PubMed

    Meyer, F

    1983-01-01

    The last years have seen an explosion of systems in image analysis. It is hard for the pathologist or the cytologist to make the right choice of equipment. All machines are stupid, and the only valuable thing is the human work put into it. So make your benefit of the work other people have done for you. Chose a method largely used on many systems and which has proved fertile in many domains and not only for your specific to day's application: Mathematical Morphology, to which are to be added the linear convolutions present on all machines is a strong candidate for becoming such a method. The paper illustrates a working day of an ideal system: research and diagnostic directed work during the working hours, automatic screening of cervical (or other) smears during night.

  3. Spreadsheet-like image analysis

    NASA Astrophysics Data System (ADS)

    Wilson, Paul

    1992-08-01

    This report describes the design of a new software system being built by the Army to support and augment automated nondestructive inspection (NDI) on-line equipment implemented by the Army for detection of defective manufactured items. The new system recalls and post-processes (off-line) the NDI data sets archived by the on-line equipment for the purpose of verifying the correctness of the inspection analysis paradigms, of developing better analysis paradigms and to gather statistics on the defects of the items inspected. The design of the system is similar to that of a spreadsheet, i.e., an array of cells which may be programmed to contain functions with arguments being data from other cells and whose resultant is the output of that cell's function. Unlike a spreadsheet, the arguments and the resultants of a cell may be a matrix such as a two-dimensional matrix of picture elements (pixels). Functions include matrix mathematics, neural networks and image processing as well as those ordinarily found in spreadsheets. The system employs all of the common environmental supports of the Macintosh computer, which is the hardware platform. The system allows the resultant of a cell to be displayed in any of multiple formats such as a matrix of numbers, text, an image, or a chart. Each cell is a window onto the resultant. Like a spreadsheet if the input value of any cell is changed its effect is cascaded into the resultants of all cells whose functions use that value directly or indirectly. The system encourages the user to play what-of games, as ordinary spreadsheets do.

  4. An Analysis of the Defense Acquisition Strategy for Unmanned Systems

    DTIC Science & Technology

    2013-11-20

    improvements in existing components and build on the existing technological trajectory, whereas exploratory innovation involves a shift to a different...also limits the comparative analysis of technology S-curves. While it is easy to assess the impact of historical technological breakthroughs, the...identify technological maturity may result in retaining the increasingly obsolete technology too long. Failure to adopt a strategy for switching

  5. A rational analysis of the acquisition of multisensory representations.

    PubMed

    Yildirim, Ilker; Jacobs, Robert A

    2012-03-01

    How do people learn multisensory, or amodal, representations, and what consequences do these representations have for perceptual performance? We address this question by performing a rational analysis of the problem of learning multisensory representations. This analysis makes use of a Bayesian nonparametric model that acquires latent multisensory features that optimally explain the unisensory features arising in individual sensory modalities. The model qualitatively accounts for several important aspects of multisensory perception: (a) it integrates information from multiple sensory sources in such a way that it leads to superior performances in, for example, categorization tasks; (b) its performances suggest that multisensory training leads to better learning than unisensory training, even when testing is conducted in unisensory conditions; (c) its multisensory representations are modality invariant; and (d) it predicts ''missing" sensory representations in modalities when the input to those modalities is absent. Our rational analysis indicates that all of these aspects emerge as part of the optimal solution to the problem of learning to represent complex multisensory environments.

  6. Naval Signal and Image Analysis Conference Report

    DTIC Science & Technology

    1998-02-26

    Arlington Hilton Hotel in Arlington, Virginia. The meeting was by invitation only and consisted of investigators in the ONR Signal and Image Analysis Program...in signal and image analysis . The conference provided an opportunity for technical interaction between academic researchers and Naval scientists and...plan future directions for the ONR Signal and Image Analysis Program as well as informal recommendations to the Program Officer.

  7. Dosimetric and image quality assessment of different acquisition protocols of a novel 64-slice CT scanner

    NASA Astrophysics Data System (ADS)

    Vite, Cristina; Mangini, Monica; Strocchi, Sabina; Novario, Raffaele; Tanzi, Fabio; Carrafiello, Gianpaolo; Conte, Leopoldo; Fugazzola, Carlo

    2006-03-01

    sharp fall below that value. A significant decrease in the effective dose to the patient, around 40%, was found; image quality analysis shows a further 10% dose reduction possibility.

  8. SPLASSH: Open source software for camera-based high-speed, multispectral in-vivo optical image acquisition.

    PubMed

    Sun, Ryan; Bouchard, Matthew B; Hillman, Elizabeth M C

    2010-08-02

    Camera-based in-vivo optical imaging can provide detailed images of living tissue that reveal structure, function, and disease. High-speed, high resolution imaging can reveal dynamic events such as changes in blood flow and responses to stimulation. Despite these benefits, commercially available scientific cameras rarely include software that is suitable for in-vivo imaging applications, making this highly versatile form of optical imaging challenging and time-consuming to implement. To address this issue, we have developed a novel, open-source software package to control high-speed, multispectral optical imaging systems. The software integrates a number of modular functions through a custom graphical user interface (GUI) and provides extensive control over a wide range of inexpensive IEEE 1394 Firewire cameras. Multispectral illumination can be incorporated through the use of off-the-shelf light emitting diodes which the software synchronizes to image acquisition via a programmed microcontroller, allowing arbitrary high-speed illumination sequences. The complete software suite is available for free download. Here we describe the software's framework and provide details to guide users with development of this and similar software.

  9. Satellite image analysis using neural networks

    NASA Technical Reports Server (NTRS)

    Sheldon, Roger A.

    1990-01-01

    The tremendous backlog of unanalyzed satellite data necessitates the development of improved methods for data cataloging and analysis. Ford Aerospace has developed an image analysis system, SIANN (Satellite Image Analysis using Neural Networks) that integrates the technologies necessary to satisfy NASA's science data analysis requirements for the next generation of satellites. SIANN will enable scientists to train a neural network to recognize image data containing scenes of interest and then rapidly search data archives for all such images. The approach combines conventional image processing technology with recent advances in neural networks to provide improved classification capabilities. SIANN allows users to proceed through a four step process of image classification: filtering and enhancement, creation of neural network training data via application of feature extraction algorithms, configuring and training a neural network model, and classification of images by application of the trained neural network. A prototype experimentation testbed was completed and applied to climatological data.

  10. SU-E-J-112: The Impact of Cine EPID Image Acquisition Frame Rate On Markerless Soft-Tissue Tracking

    SciTech Connect

    Yip, S; Rottmann, J; Berbeco, R

    2014-06-01

    Purpose: Although reduction of the cine EPID acquisition frame rate through multiple frame averaging may reduce hardware memory burden and decrease image noise, it can hinder the continuity of soft-tissue motion leading to poor auto-tracking results. The impact of motion blurring and image noise on the tracking performance was investigated. Methods: Phantom and patient images were acquired at a frame rate of 12.87Hz on an AS1000 portal imager. Low frame rate images were obtained by continuous frame averaging. A previously validated tracking algorithm was employed for auto-tracking. The difference between the programmed and auto-tracked positions of a Las Vegas phantom moving in the superior-inferior direction defined the tracking error (δ). Motion blurring was assessed by measuring the area change of the circle with the greatest depth. Additionally, lung tumors on 1747 frames acquired at eleven field angles from four radiotherapy patients are manually and automatically tracked with varying frame averaging. δ was defined by the position difference of the two tracking methods. Image noise was defined as the standard deviation of the background intensity. Motion blurring and image noise were correlated with δ using Pearson correlation coefficient (R). Results: For both phantom and patient studies, the auto-tracking errors increased at frame rates lower than 4.29Hz. Above 4.29Hz, changes in errors were negligible with δ<1.60mm. Motion blurring and image noise were observed to increase and decrease with frame averaging, respectively. Motion blurring and tracking errors were significantly correlated for the phantom (R=0.94) and patient studies (R=0.72). Moderate to poor correlation was found between image noise and tracking error with R -0.58 and -0.19 for both studies, respectively. Conclusion: An image acquisition frame rate of at least 4.29Hz is recommended for cine EPID tracking. Motion blurring in images with frame rates below 4.39Hz can substantially reduce the

  11. Microscopy image segmentation tool: Robust image data analysis

    SciTech Connect

    Valmianski, Ilya Monton, Carlos; Schuller, Ivan K.

    2014-03-15

    We present a software package called Microscopy Image Segmentation Tool (MIST). MIST is designed for analysis of microscopy images which contain large collections of small regions of interest (ROIs). Originally developed for analysis of porous anodic alumina scanning electron images, MIST capabilities have been expanded to allow use in a large variety of problems including analysis of biological tissue, inorganic and organic film grain structure, as well as nano- and meso-scopic structures. MIST provides a robust segmentation algorithm for the ROIs, includes many useful analysis capabilities, and is highly flexible allowing incorporation of specialized user developed analysis. We describe the unique advantages MIST has over existing analysis software. In addition, we present a number of diverse applications to scanning electron microscopy, atomic force microscopy, magnetic force microscopy, scanning tunneling microscopy, and fluorescent confocal laser scanning microscopy.

  12. Microscopy image segmentation tool: robust image data analysis.

    PubMed

    Valmianski, Ilya; Monton, Carlos; Schuller, Ivan K

    2014-03-01

    We present a software package called Microscopy Image Segmentation Tool (MIST). MIST is designed for analysis of microscopy images which contain large collections of small regions of interest (ROIs). Originally developed for analysis of porous anodic alumina scanning electron images, MIST capabilities have been expanded to allow use in a large variety of problems including analysis of biological tissue, inorganic and organic film grain structure, as well as nano- and meso-scopic structures. MIST provides a robust segmentation algorithm for the ROIs, includes many useful analysis capabilities, and is highly flexible allowing incorporation of specialized user developed analysis. We describe the unique advantages MIST has over existing analysis software. In addition, we present a number of diverse applications to scanning electron microscopy, atomic force microscopy, magnetic force microscopy, scanning tunneling microscopy, and fluorescent confocal laser scanning microscopy.

  13. Acquisition and analysis of road incidents based on vehicle dynamics.

    PubMed

    Naude, Claire; Serre, Thierry; Dubois-Lounis, Maxime; Fournier, Jean-Yves; Lechner, Daniel; Guilbot, Michèle; Ledoux, Vincent

    2017-03-09

    Because motor vehicle crashes have decreased during the last decade in many countries in the world and are more diffuse, local authorities have difficulties to define road safety policies. An experiment with 51 cars of public fleets equipped with a specific Event Data Recorder was carried out in France during one year. The purposes of this research were to evaluate if incident data (critical driving situations) help to understand crashes, and to explore a new way for road infrastructure safety diagnosis. The analysis of 339 genuine incidents and 1237 simple events recorded illustrates the potentiality of such an experiment and provides: some insights about conditions in which incidents occur, a general overview of their distribution according to different road layouts, as well as information on the different levels of accelerations reached. It can be noticed that there is an overrepresentation of incidents in right curves compared to left curves. The simple events involving mostly the infrastructure could be used to detect road defects. Genuine incidents where the vehicle is subjected to important dynamic demands, related to potentially unsafe driving situations, can be used to improve knowledge of the motor vehicle crashes thanks to incident mechanisms analysis.

  14. Digital breast tomosynthesis: studies of the effects of acquisition geometry on contrast-to-noise ratio and observer preference of low-contrast objects in breast phantom images

    NASA Astrophysics Data System (ADS)

    Goodsitt, Mitchell M.; Chan, Heang-Ping; Schmitz, Andrea; Zelakiewicz, Scott; Telang, Santosh; Hadjiiski, Lubomir; Watcharotone, Kuanwong; Helvie, Mark A.; Paramagul, Chintana; Neal, Colleen; Christodoulou, Emmanuel; Larson, Sandra C.; Carson, Paul L.

    2014-10-01

    The effect of acquisition geometry in digital breast tomosynthesis was evaluated with studies of contrast-to-noise ratios (CNRs) and observer preference. Contrast-detail (CD) test objects in 5 cm thick phantoms with breast-like backgrounds were imaged. Twelve different angular acquisitions (average glandular dose for each ~1.1 mGy) were performed ranging from narrow angle 16° with 17 projection views (16d17p) to wide angle 64d17p. Focal slices of SART-reconstructed images of the CD arrays were selected for CNR computations and the reader preference study. For the latter, pairs of images obtained with different acquisition geometries were randomized and scored by 7 trained readers. The total scores for all images and readings for each acquisition geometry were compared as were the CNRs. In general, readers preferred images acquired with wide angle as opposed to narrow angle geometries. The mean percent preferred was highly correlated with tomosynthesis angle (R = 0.91). The highest scoring geometries were 60d21p (95%), 64d17p (80%), and 48d17p (72%); the lowest scoring were 16d17p (4%), 24d9p (17%) and 24d13p (33%). The measured CNRs for the various acquisitions showed much overlap but were overall highest for wide-angle acquisitions. Finally, the mean reader scores were well correlated with the mean CNRs (R = 0.83).

  15. MIRD pamphlet no. 16: Techniques for quantitative radiopharmaceutical biodistribution data acquisition and analysis for use in human radiation dose estimates.

    PubMed

    Siegel, J A; Thomas, S R; Stubbs, J B; Stabin, M G; Hays, M T; Koral, K F; Robertson, J S; Howell, R W; Wessels, B W; Fisher, D R; Weber, D A; Brill, A B

    1999-02-01

    This report describes recommended techniques for radiopharmaceutical biodistribution data acquisition and analysis in human subjects to estimate radiation absorbed dose using the Medical Internal Radiation Dose (MIRD) schema. The document has been prepared in a format to address two audiences: individuals with a primary interest in designing clinical trials who are not experts in dosimetry and individuals with extensive experience with dosimetry-based protocols and calculational methodology. For the first group, the general concepts involved in biodistribution data acquisition are presented, with guidance provided for the number of measurements (data points) required. For those with expertise in dosimetry, highlighted sections, examples and appendices have been included to provide calculational details, as well as references, for the techniques involved. This document is intended also to serve as a guide for the investigator in choosing the appropriate methodologies when acquiring and preparing product data for review by national regulatory agencies. The emphasis is on planar imaging techniques commonly available in most nuclear medicine departments and laboratories. The measurement of the biodistribution of radiopharmaceuticals is an important aspect in calculating absorbed dose from internally deposited radionuclides. Three phases are presented: data collection, data analysis and data processing. In the first phase, data collection, the identification of source regions, the determination of their appropriate temporal sampling and the acquisition of data are discussed. In the second phase, quantitative measurement techniques involving imaging by planar scintillation camera, SPECT and PET for the calculation of activity in source regions as a function of time are discussed. In addition, nonimaging measurement techniques, including external radiation monitoring, tissue-sample counting (blood and biopsy) and excreta counting are also considered. The third phase, data

  16. Image processing software for imaging spectrometry data analysis

    NASA Technical Reports Server (NTRS)

    Mazer, Alan; Martin, Miki; Lee, Meemong; Solomon, Jerry E.

    1988-01-01

    Imaging spectrometers simultaneously collect image data in hundreds of spectral channels, from the near-UV to the IR, and can thereby provide direct surface materials identification by means resembling laboratory reflectance spectroscopy. Attention is presently given to a software system, the Spectral Analysis Manager (SPAM) for the analysis of imaging spectrometer data. SPAM requires only modest computational resources and is composed of one main routine and a set of subroutine libraries. Additions and modifications are relatively easy, and special-purpose algorithms have been incorporated that are tailored to geological applications.

  17. Image processing software for imaging spectrometry data analysis

    NASA Astrophysics Data System (ADS)

    Mazer, Alan; Martin, Miki; Lee, Meemong; Solomon, Jerry E.

    1988-02-01

    Imaging spectrometers simultaneously collect image data in hundreds of spectral channels, from the near-UV to the IR, and can thereby provide direct surface materials identification by means resembling laboratory reflectance spectroscopy. Attention is presently given to a software system, the Spectral Analysis Manager (SPAM) for the analysis of imaging spectrometer data. SPAM requires only modest computational resources and is composed of one main routine and a set of subroutine libraries. Additions and modifications are relatively easy, and special-purpose algorithms have been incorporated that are tailored to geological applications.

  18. Standardization of infrared breast thermogram acquisition protocols and abnormality analysis of breast thermograms

    NASA Astrophysics Data System (ADS)

    Bhowmik, Mrinal Kanti; Gogoi, Usha Rani; Das, Kakali; Ghosh, Anjan Kumar; Bhattacharjee, Debotosh; Majumdar, Gautam

    2016-05-01

    The non-invasive, painless, radiation-free and cost-effective infrared breast thermography (IBT) makes a significant contribution to improving the survival rate of breast cancer patients by early detecting the disease. This paper presents a set of standard breast thermogram acquisition protocols to improve the potentiality and accuracy of infrared breast thermograms in early breast cancer detection. By maintaining all these protocols, an infrared breast thermogram acquisition setup has been established at the Regional Cancer Centre (RCC) of Government Medical College (AGMC), Tripura, India. The acquisition of breast thermogram is followed by the breast thermogram interpretation, for identifying the presence of any abnormality. However, due to the presence of complex vascular patterns, accurate interpretation of breast thermogram is a very challenging task. The bilateral symmetry of the thermal patterns in each breast thermogram is quantitatively computed by statistical feature analysis. A series of statistical features are extracted from a set of 20 thermograms of both healthy and unhealthy subjects. Finally, the extracted features are analyzed for breast abnormality detection. The key contributions made by this paper can be highlighted as -- a) the designing of a standard protocol suite for accurate acquisition of breast thermograms, b) creation of a new breast thermogram dataset by maintaining the protocol suite, and c) statistical analysis of the thermograms for abnormality detection. By doing so, this proposed work can minimize the rate of false findings in breast thermograms and thus, it will increase the utilization potentiality of breast thermograms in early breast cancer detection.

  19. System Matrix Analysis for Computed Tomography Imaging.

    PubMed

    Flores, Liubov; Vidal, Vicent; Verdú, Gumersindo

    2015-01-01

    In practical applications of computed tomography imaging (CT), it is often the case that the set of projection data is incomplete owing to the physical conditions of the data acquisition process. On the other hand, the high radiation dose imposed on patients is also undesired. These issues demand that high quality CT images can be reconstructed from limited projection data. For this reason, iterative methods of image reconstruction have become a topic of increased research interest. Several algorithms have been proposed for few-view CT. We consider that the accurate solution of the reconstruction problem also depends on the system matrix that simulates the scanning process. In this work, we analyze the application of the Siddon method to generate elements of the matrix and we present results based on real projection data.

  20. System Matrix Analysis for Computed Tomography Imaging

    PubMed Central

    Flores, Liubov; Vidal, Vicent; Verdú, Gumersindo

    2015-01-01

    In practical applications of computed tomography imaging (CT), it is often the case that the set of projection data is incomplete owing to the physical conditions of the data acquisition process. On the other hand, the high radiation dose imposed on patients is also undesired. These issues demand that high quality CT images can be reconstructed from limited projection data. For this reason, iterative methods of image reconstruction have become a topic of increased research interest. Several algorithms have been proposed for few-view CT. We consider that the accurate solution of the reconstruction problem also depends on the system matrix that simulates the scanning process. In this work, we analyze the application of the Siddon method to generate elements of the matrix and we present results based on real projection data. PMID:26575482

  1. Quantitative Computed Tomography and Image Analysis for Advanced Muscle Assessment

    PubMed Central

    Edmunds, Kyle Joseph; Gíslason, Magnus K.; Arnadottir, Iris D.; Marcante, Andrea; Piccione, Francesco; Gargiulo, Paolo

    2016-01-01

    Medical imaging is of particular interest in the field of translational myology, as extant literature describes the utilization of a wide variety of techniques to non-invasively recapitulate and quantity various internal and external tissue morphologies. In the clinical context, medical imaging remains a vital tool for diagnostics and investigative assessment. This review outlines the results from several investigations on the use of computed tomography (CT) and image analysis techniques to assess muscle conditions and degenerative process due to aging or pathological conditions. Herein, we detail the acquisition of spiral CT images and the use of advanced image analysis tools to characterize muscles in 2D and 3D. Results from these studies recapitulate changes in tissue composition within muscles, as visualized by the association of tissue types to specified Hounsfield Unit (HU) values for fat, loose connective tissue or atrophic muscle, and normal muscle, including fascia and tendon. We show how results from these analyses can be presented as both average HU values and compositions with respect to total muscle volumes, demonstrating the reliability of these tools to monitor, assess and characterize muscle degeneration. PMID:27478562

  2. SU-F-303-02: Achieving 4D MRI in Regular Breathing Cycle with Extended Acquisition Time of Dynamic MR Images

    SciTech Connect

    Hui, C; Beddar, S; Wen, Z; Stemkens, B; Tijssen, R; Berg, C van den

    2015-06-15

    Purpose: The purpose of this study is to develop a technique to obtain four-dimensional (4D) magnetic resonance (MR) images that are more representative of a patient’s typical breathing cycle by utilizing an extended acquisition time while minimizing the image artifacts. Methods: The 4D MR data were acquired with the balanced steady state free precession in two-dimensional sagittal plane of view. Each slice was acquired repeatedly for about 15 s, thereby obtaining multiple images at each of the 10 phases in the respiratory cycle. This improves the probability that at least one of the images were acquired at the desired phase during a regular breathing cycle. To create optimal 4D MR images, an iterative approach was used to identify the set of images that yielded the highest slice-to-slice similarity. To assess the effectiveness of the approach, the data set was truncated into periods of 7 s (50 time points), 11 s (75 time points) and the full 15 s (100 time points). The 4D MR images were then sorted with data of the three different acquisition periods for comparison. Results: In general, the 4D MR images sorted using data from longer acquisition periods showed less mismatched artifacts. In addition, the normalized cross correlation (NCC) between slices of a 4D volume increases with increased acquisition period. The average NCC was 0.791 from the 7 s period, 0.794 from the 11 s period and 0.796 from the 15 s period. Conclusion: Our preliminary study showed that extending the acquisition time with the proposed sorting technique can improve image quality and reduce artifact presence in the 4D MR images. Data acquisition over two breathing cycles is a good trade-off between artifact reduction and scan time. This research was partially funded by the the Center for Radiation Oncology Research from UT MD Anderson Cancer Center.

  3. Proposed military handbook for dynamic data acquisition and analysis - An invitation to review

    NASA Technical Reports Server (NTRS)

    Himelblau, Harry; Wise, James H.; Piersol, Allan G.; Grundvig, Max R.

    1990-01-01

    A draft Military Handbook prepared under the sponsorship of the USAF Space Division is presently being distributed throughout the U.S. for review by the aerospace community. This comprehensive document provides recommended guidelines for the acquisition and analysis of structural dynamics and aeroacoustic data, and is intended to reduce the errors and variability commonly found in flight, ground and laboratory dynamic test measurements. In addition to the usual variety of measurement problems encountered in the definition of dynamic loads, the development of design and test criteria, and the analysis of failures, special emphasis is given to certain state-of-the-art topics, such as pyroshock data acquisition and nonstationary random data analysis.

  4. Image registration with uncertainty analysis

    DOEpatents

    Simonson, Katherine M [Cedar Crest, NM

    2011-03-22

    In an image registration method, edges are detected in a first image and a second image. A percentage of edge pixels in a subset of the second image that are also edges in the first image shifted by a translation is calculated. A best registration point is calculated based on a maximum percentage of edges matched. In a predefined search region, all registration points other than the best registration point are identified that are not significantly worse than the best registration point according to a predetermined statistical criterion.

  5. On the acquisition and analysis of microscale thermophoresis data.

    PubMed

    Scheuermann, Thomas H; Padrick, Shae B; Gardner, Kevin H; Brautigam, Chad A

    2016-03-01

    A comprehensive understanding of the molecular mechanisms underpinning cellular functions is dependent on a detailed characterization of the energetics of macromolecular binding, often quantified by the equilibrium dissociation constant, KD. While many biophysical methods may be used to obtain KD, the focus of this report is a relatively new method called microscale thermophoresis (MST). In an MST experiment, a capillary tube filled with a solution containing a dye-labeled solute is illuminated with an infrared laser, rapidly creating a temperature gradient. Molecules will migrate along this gradient, causing changes in the observed fluorescence. Because the net migration of the labeled molecules will depend on their liganded state, a binding curve as a function of ligand concentration can be constructed from MST data and analyzed to determine KD. Herein, simulations demonstrate the limits of KD that can be measured in current instrumentation. They also show that binding kinetics is a major concern in planning and executing MST experiments. Additionally, studies of two protein-protein interactions illustrate challenges encountered in acquiring and analyzing MST data. Combined, these approaches indicate a set of best practices for performing and analyzing MST experiments. Software for rigorous data analysis is also introduced.

  6. On the acquisition and analysis of microscale thermophoresis data

    PubMed Central

    Scheuermann, Thomas H.; Padrick, Shae B.; Gardner, Kevin H.; Brautigam, Chad A.

    2016-01-01

    A comprehensive understanding of the molecular mechanisms underpinning cellular functions is dependent on a detailed characterization of the energetics of macromolecular binding, often quantified by the equilibrium dissociation constant, KD. While many biophysical methods may be used to obtain KD, the focus of this report is a relatively new method called “microscale thermophoresis” (MST). In an MST experiment, a capillary tube filled with a solution containing a dye-labeled solute is illuminated with an infrared laser, rapidly creating a temperature gradient. Molecules will migrate along this gradient, causing changes in the observed fluorescence. Because the net migration of the labeled molecules will depend on their liganded state, a binding curve can be constructed as a function of ligand concentration from MST data and analyzed to determine KD. Herein, simulations demonstrate the limits of KD that can be measured in current instrumentation. They also show that binding kinetics are a major concern when planning and executing MST experiments. Additionally, studies of two protein-protein interactions illustrate challenges encountered in acquiring and analyzing MST data. Combined, these approaches indicate a set of best practices for performing and analyzing MST experiments. Software for rigorous data analysis is also introduced. PMID:26739938

  7. Light calibration and quality assessment methods for Reflectance Transformation Imaging applied to artworks' analysis

    NASA Astrophysics Data System (ADS)

    Giachetti, A.; Daffara, C.; Reghelin, C.; Gobbetti, E.; Pintus, R.

    2015-06-01

    In this paper we analyze some problems related to the acquisition of multiple illumination images for Polynomial Texture Maps (PTM) or generic Reflectance Transform Imaging (RTI). We show that intensity and directionality nonuniformity can be a relevant issue when acquiring manual sets of images with the standard highlight-based setup both using a flash lamp and a LED light. To maintain a cheap and flexible acquisition setup that can be used on field and by non-experienced users we propose to use a dynamic calibration and correction of the lights based on multiple intensity and direction estimation around the imaged object during the acquisition. Preliminary tests on the results obtained have been performed by acquiring a specifically designed 3D printed pattern in order to see the accuracy of the acquisition obtained both for spatial discrimination of small structures and normal estimation, and on samples of different types of paper in order to evaluate material discrimination. We plan to design and build from our analysis and from the tools developed and under development a set of novel procedures and guidelines that can be used to turn the cheap and common RTI acquisition setup from a simple way to enrich object visualization into a powerful method for extracting quantitative characterization both of surface geometry and of reflective properties of different materials. These results could have relevant applications in the Cultural Heritage domain, in order to recognize different materials used in paintings or investigate the ageing status of artifacts' surface.

  8. Hyperspectral image classification using functional data analysis.

    PubMed

    Li, Hong; Xiao, Guangrun; Xia, Tian; Tang, Y Y; Li, Luoqing

    2014-09-01

    The large number of spectral bands acquired by hyperspectral imaging sensors allows us to better distinguish many subtle objects and materials. Unlike other classical hyperspectral image classification methods in the multivariate analysis framework, in this paper, a novel method using functional data analysis (FDA) for accurate classification of hyperspectral images has been proposed. The central idea of FDA is to treat multivariate data as continuous functions. From this perspective, the spectral curve of each pixel in the hyperspectral images is naturally viewed as a function. This can be beneficial for making full use of the abundant spectral information. The relevance between adjacent pixel elements in the hyperspectral images can also be utilized reasonably. Functional principal component analysis is applied to solve the classification problem of these functions. Experimental results on three hyperspectral images show that the proposed method can achieve higher classification accuracies in comparison to some state-of-the-art hyperspectral image classification methods.

  9. Integration of real-time 3D image acquisition and multiview 3D display

    NASA Astrophysics Data System (ADS)

    Zhang, Zhaoxing; Geng, Zheng; Li, Tuotuo; Li, Wei; Wang, Jingyi; Liu, Yongchun

    2014-03-01

    Seamless integration of 3D acquisition and 3D display systems offers enhanced experience in 3D visualization of the real world objects or scenes. The vivid representation of captured 3D objects displayed on a glasses-free 3D display screen could bring the realistic viewing experience to viewers as if they are viewing real-world scene. Although the technologies in 3D acquisition and 3D display have advanced rapidly in recent years, effort is lacking in studying the seamless integration of these two different aspects of 3D technologies. In this paper, we describe our recent progress on integrating a light-field 3D acquisition system and an autostereoscopic multiview 3D display for real-time light field capture and display. This paper focuses on both the architecture design and the implementation of the hardware and the software of this integrated 3D system. A prototype of the integrated 3D system is built to demonstrate the real-time 3D acquisition and 3D display capability of our proposed system.

  10. Acquisition and analysis of angle-beam wavefield data

    SciTech Connect

    Dawson, Alexander J.; Michaels, Jennifer E.; Levine, Ross M.; Chen, Xin; Michaels, Thomas E.

    2014-02-18

    Angle-beam ultrasonic testing is a common practical technique used for nondestructive evaluation to detect, locate, and characterize a variety of material defects and damage. Greater understanding of the both the incident wavefield produced by an angle-beam transducer and the subsequent scattering from a variety of defects and geometrical features is anticipated to increase the reliability of data interpretation. The focus of this paper is on acquiring and analyzing propagating waves from angle-beam transducers in simple, defect-free plates as a first step in the development of methods for flaw characterization. Unlike guided waves, which excite the plate throughout its thickness, angle-beam bulk waves bounce back and forth between the plate surfaces, resulting in the well-known multiple “skips” or “V-paths.” The experimental setup consists of a laser vibrometer mounted on an XYZ scanning stage, which is programmed to move point-to-point on a rectilinear grid to acquire waveform data. Although laser vibrometry is now routinely used to record guided waves for which the frequency content is below 1 MHz, it is more challenging to acquire higher frequency bulk waves in the 1–10 MHz range. Signals are recorded on the surface of an aluminum plate that were generated from a 5 MHz, 65° refracted angle, shear wave transducer-wedge combination. Data are analyzed directly in the x-t domain, via a slant stack Radon transform in the τ-p (offset time-slowness) domain, and via a 2-D Fourier transform in the ω-k domain, thereby enabling identification of specific arrivals and modes. Results compare well to those expected from a simple ray tracing analysis except for the unexpected presence of a strong Rayleigh wave.

  11. Study on clear stereo image pair acquisition method for small objects with big vertical size in SLM vision system.

    PubMed

    Wang, Yuezong; Jin, Yan; Wang, Lika; Geng, Benliang

    2016-05-01

    Microscopic vision system with stereo light microscope (SLM) has been applied to surface profile measurement. If the vertical size of a small object exceeds the range of depth, its images will contain clear and fuzzy image regions. Hence, in order to obtain clear stereo images, we propose a microscopic sequence image fusion method which is suitable for SLM vision system. First, a solution to capture and align image sequence is designed, which outputs an aligning stereo images. Second, we decompose stereo image sequence by wavelet analysis theory, and obtain a series of high and low frequency coefficients with different resolutions. Then fused stereo images are output based on the high and low frequency coefficient fusion rules proposed in this article. The results show that Δw1 (Δw2 ) and ΔZ of stereo images in a sequence have linear relationship. Hence, a procedure for image alignment is necessary before image fusion. In contrast with other image fusion methods, our method can output clear fused stereo images with better performance, which is suitable for SLM vision system, and very helpful for avoiding image fuzzy caused by big vertical size of small objects.

  12. Inverse Process Analysis for the Acquisition of Thermophysical Data

    SciTech Connect

    Jay Frankel; Adrian Sabau

    2004-10-31

    One of the main barriers in the analysis and design of materials processing and industrial applications is the lack of accurate experimental data on the thermophysical properties of materials. To date, the measurement of most of these high-temperature thermophysical properties has often been plagued by temperature lags that are inherent in measurement techniques. These lags can be accounted for with the appropriate mathematical models, reflecting the experimental apparatus and sample region, in order to deduce the desired measurement as a function of true sample temperature. Differential scanning calorimeter (DSC) measurements are routinely used to determine enthalpies of phase change, phase transition temperatures, glass transition temperatures, and heat capacities. In the aluminum, steel, and metal casting industries, predicting the formation of defects such as shrinkage voids, microporosity, and macrosegregation is limited by the data available on fraction solid and density evolution during solidification. Dilatometer measurements are routinely used to determine the density of a sample at various temperatures. An accurate determination of the thermophysical properties of materials is needed to achieve accuracy in the numerical simulations used to improve or design new material processes. In most of the instruments used to measure properties, the temperature is changed according to instrument controllers and there is a nonhomogeneous temperature distribution within the instrument. Additionally, the sample temperature cannot be measured directly: temperature data are collected from a thermocouple that is placed at a different location than that of the sample, thus introducing a time lag. The goal of this project was to extend the utility, quality and accuracy of two types of commercial instruments -a DSC and a dilatometer - used for thermophysical property measurements in high-temperature environments. In particular, the quantification of solid fraction and

  13. apART: system for the acquisition, processing, archiving, and retrieval of digital images in an open, distributed imaging environment

    NASA Astrophysics Data System (ADS)

    Schneider, Uwe; Strack, Ruediger

    1992-04-01

    apART reflects the structure of an open, distributed environment. According to the general trend in the area of imaging, network-capable, general purpose workstations with capabilities of open system image communication and image input are used. Several heterogeneous components like CCD cameras, slide scanners, and image archives can be accessed. The system is driven by an object-oriented user interface where devices (image sources and destinations), operators (derived from a commercial image processing library), and images (of different data types) are managed and presented uniformly to the user. Browsing mechanisms are used to traverse devices, operators, and images. An audit trail mechanism is offered to record interactive operations on low-resolution image derivatives. These operations are processed off-line on the original image. Thus, the processing of extremely high-resolution raster images is possible, and the performance of resolution dependent operations is enhanced significantly during interaction. An object-oriented database system (APRIL), which can be browsed, is integrated into the system. Attribute retrieval is supported by the user interface. Other essential features of the system include: implementation on top of the X Window System (X11R4) and the OSF/Motif widget set; a SUN4 general purpose workstation, inclusive ethernet, magneto optical disc, etc., as the hardware platform for the user interface; complete graphical-interactive parametrization of all operators; support of different image interchange formats (GIF, TIFF, IIF, etc.); consideration of current IPI standard activities within ISO/IEC for further refinement and extensions.

  14. VPI - VIBRATION PATTERN IMAGER: A CONTROL AND DATA ACQUISITION SYSTEM FOR SCANNING LASER VIBROMETERS

    NASA Technical Reports Server (NTRS)

    Rizzi, S. A.

    1994-01-01

    The Vibration Pattern Imager (VPI) system was designed to control and acquire data from laser vibrometer sensors. The PC computer based system uses a digital signal processing (DSP) board and an analog I/O board to control the sensor and to process the data. The VPI system was originally developed for use with the Ometron VPI Sensor (Ometron Limited, Kelvin House, Worsley Bridge Road, London, SE26 5BX, England), but can be readily adapted to any commercially available sensor which provides an analog output signal and requires analog inputs for control of mirror positioning. VPI's graphical user interface allows the operation of the program to be controlled interactively through keyboard and mouse-selected menu options. The main menu controls all functions for setup, data acquisition, display, file operations, and exiting the program. Two types of data may be acquired with the VPI system: single point or "full field". In the single point mode, time series data is sampled by the A/D converter on the I/O board at a user-defined rate for the selected number of samples. The position of the measuring point, adjusted by mirrors in the sensor, is controlled via a mouse input. In the "full field" mode, the measurement point is moved over a user-selected rectangular area with up to 256 positions in both x and y directions. The time series data is sampled by the A/D converter on the I/O board and converted to a root-mean-square (rms) value by the DSP board. The rms "full field" velocity distribution is then uploaded for display and storage. VPI is written in C language and Texas Instruments' TMS320C30 assembly language for IBM PC series and compatible computers running MS-DOS. The program requires 640K of RAM for execution, and a hard disk with 10Mb or more of disk space is recommended. The program also requires a mouse, a VGA graphics display, a Four Channel analog I/O board (Spectrum Signal Processing, Inc.; Westborough, MA), a break-out box and a Spirit-30 board (Sonitech

  15. A Mathematical Framework for Image Analysis

    DTIC Science & Technology

    1991-08-01

    The results reported here were derived from the research project ’A Mathematical Framework for Image Analysis ’ supported by the Office of Naval...Research, contract N00014-88-K-0289 to Brown University. A common theme for the work reported is the use of probabilistic methods for problems in image ... analysis and image reconstruction. Five areas of research are described: rigid body recognition using a decision tree/combinatorial approach; nonrigid

  16. High-description image acquisition for digital archiving of rare books

    NASA Astrophysics Data System (ADS)

    Kashimura, Masaaki; Nakajima, Toshifumi; Maeda, Taizo; Onda, Norikazu; Saito, Hideo; Ozawa, Shinji

    1998-12-01

    At first in this paper, we given an outline of activity of the Humanities Media Interface (HUMI) Project. This project was established by Keio University for the purpose of digital archiving of rare books held in Keio University Library, and of realizing a research oriented digital library. Then our way of acquiring rare book images of super high definition is introduced and image compensation method for acquiring just- front view of page using the 3-D information extracted from the shape of top line of the page area depicted in the image is proposed. Our approach of acquiring higher resolution image by joining close-up partial images of a page is also introduced. The proposing image adjustment method is extended for partial images of a page as preprocess of joining them together. In the experiment, well-adjusted and joined page images could be obtained.

  17. Imageability Predicts the Age of Acquisition of Verbs in Chinese Children

    ERIC Educational Resources Information Center

    Ma, Weiyi; Golinkoff, Roberta Michnick; Hirsh-Pasek, Kathy; McDonough, Colleen; Tardif, Twila

    2009-01-01

    Verbs are harder to learn than nouns in English and in many other languages, but are relatively easy to learn in Chinese. This paper evaluates one potential explanation for these findings by examining the construct of imageability, or the ability of a word to produce a mental image. Chinese adults rated the imageability of Chinese words from the…

  18. Image Reconstruction Using Analysis Model Prior

    PubMed Central

    Han, Yu; Du, Huiqian; Lam, Fan; Mei, Wenbo; Fang, Liping

    2016-01-01

    The analysis model has been previously exploited as an alternative to the classical sparse synthesis model for designing image reconstruction methods. Applying a suitable analysis operator on the image of interest yields a cosparse outcome which enables us to reconstruct the image from undersampled data. In this work, we introduce additional prior in the analysis context and theoretically study the uniqueness issues in terms of analysis operators in general position and the specific 2D finite difference operator. We establish bounds on the minimum measurement numbers which are lower than those in cases without using analysis model prior. Based on the idea of iterative cosupport detection (ICD), we develop a novel image reconstruction model and an effective algorithm, achieving significantly better reconstruction performance. Simulation results on synthetic and practical magnetic resonance (MR) images are also shown to illustrate our theoretical claims. PMID:27379171

  19. LabVIEW: a software system for data acquisition, data analysis, and instrument control.

    PubMed

    Kalkman, C J

    1995-01-01

    Computer-based data acquisition systems play an important role in clinical monitoring and in the development of new monitoring tools. LabVIEW (National Instruments, Austin, TX) is a data acquisition and programming environment that allows flexible acquisition and processing of analog and digital data. The main feature that distinguishes LabVIEW from other data acquisition programs is its highly modular graphical programming language, "G," and a large library of mathematical and statistical functions. The advantage of graphical programming is that the code is flexible, reusable, and self-documenting. Subroutines can be saved in a library and reused without modification in other programs. This dramatically reduces development time and enables researchers to develop or modify their own programs. LabVIEW uses a large amount of processing power and computer memory, thus requiring a powerful computer. A large-screen monitor is desirable when developing larger applications. LabVIEW is excellently suited for testing new monitoring paradigms, analysis algorithms, or user interfaces. The typical LabVIEW user is the researcher who wants to develop a new monitoring technique, a set of new (derived) variables by integrating signals from several existing patient monitors, closed-loop control of a physiological variable, or a physiological simulator.

  20. An open-source, FireWire camera-based, Labview-controlled image acquisition system for automated, dynamic pupillometry and blink detection.

    PubMed

    de Souza, John Kennedy Schettino; Pinto, Marcos Antonio da Silva; Vieira, Pedro Gabrielle; Baron, Jerome; Tierra-Criollo, Carlos Julio

    2013-12-01

    The dynamic, accurate measurement of pupil size is extremely valuable for studying a large number of neuronal functions and dysfunctions. Despite tremendous and well-documented progress in image processing techniques for estimating pupil parameters, comparatively little work has been reported on practical hardware issues involved in designing image acquisition systems for pupil analysis. Here, we describe and validate the basic features of such a system which is based on a relatively compact, off-the-shelf, low-cost FireWire digital camera. We successfully implemented two configurable modes of video record: a continuous mode and an event-triggered mode. The interoperability of the whole system is guaranteed by a set of modular software components hosted on a personal computer and written in Labview. An offline analysis suite of image processing algorithms for automatically estimating pupillary and eyelid parameters were assessed using data obtained in human subjects. Our benchmark results show that such measurements can be done in a temporally precise way at a sampling frequency of up to 120 Hz and with an estimated maximum spatial resolution of 0.03 mm. Our software is made available free of charge to the scientific community, allowing end users to either use the software as is or modify it to suit their own needs.

  1. Research of Data Acquisition and Analysis System for Internal Combustion Engine Based on DSP

    NASA Astrophysics Data System (ADS)

    Gao, Y. H.; Tian, X. L.; Cheng, P.; Chang, X.; Dou, W. J.

    2006-10-01

    In the paper, the structure, working principle, functions and characteristics of an data acquisition and analysis system for internal combustion engines (I.C. engine) based on DSP is introduced. The DSP can not only acquire and analyze the data alone, also can work with the PC together to form data acquisition and analysis system with high speed and large memory. The system takes advantages of TMS320F2812's plenty of peripherals on chip, becomes small and easy for installation. USB technique is used to translate data between DSP and PC in high speed, so the system's real time processing is proved very much. It is proved that the designed system can acquire and analyze the steady and transient parameters of the I.C. engine very well.

  2. Engineering the Business of Defense Acquisition: An Analysis of Program Office Processes

    DTIC Science & Technology

    2015-04-30

    ååì~ä=^Åèìáëáíáçå= oÉëÉ~êÅÜ=póãéçëáìã= tÉÇåÉëÇ~ó=pÉëëáçåë= sçäìãÉ=f= = Engineering the Business of Defense Acquisition: An Analysis of Program Office...DATES COVERED 00-00-2015 to 00-00-2015 4. TITLE AND SUBTITLE Engineering the Business of Defense Acquisition: An Analysis of Program Office...PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Naval Postgraduate School,Graduate School of Business & Public Policy,555 Dyer Rd,Monterey,CA,93943 8

  3. A novel device with 36 channels for imaging and signal acquisition of the gastrointestinal tract based on AC biosusceptometry.

    PubMed

    Paixao, Fabiano C; Quini, Caio C; Baffa, Oswaldo; Miranda, Jose Ricardo de A

    2010-01-01

    The alternate current biosusceptometry (ACB) is a biomagnetic technique used to study some physiological parameters associated with gastrointestinal (GI) tract. For this purpose it applies an AC magnetic field and measures the response originating from magnetic marks or tracers. This paper presents an equipment based on the ACB which uses anisotropic magnetoresistive (AMR) sensors and an inexpensive electronic support. The ACB-AMR developed consists of a square array of 6×6 sensors arranged in a first-order gradiometer configuration with one reference sensor. The equipment was applied to capture magnetic images of different phantoms and to acquire gastric contraction activity of healthy rats. The results show a reasonable sensitivity and spatial-temporal resolution, so that it may be applied for imaging of phantoms and signal acquisition of the GI tract of small animals.

  4. A New Acquisition and Imaging System for Environmental Measurements: An Experience on the Italian Cultural Heritage

    PubMed Central

    Leccese, Fabio; Cagnetti, Marco; Calogero, Andrea; Trinca, Daniele; di Pasquale, Stefano; Giarnetti, Sabino; Cozzella, Lorenzo

    2014-01-01

    A new acquisition system for remote control of wall paintings has been realized and tested in the field. The system measures temperature and atmospheric pressure in an archeological site where a fresco has been put under control. The measuring chain has been designed to be used in unfavorable environments where neither electric power nor telecommunication infrastructures are available. The environmental parameters obtained from the local monitoring are then transferred remotely allowing an easier management by experts in the field of conservation of cultural heritage. The local acquisition system uses an electronic card based on microcontrollers and sends the data to a central unit realized with a Raspberry-Pi. The latter manages a high quality camera to pick up pictures of the fresco. Finally, to realize the remote control at a site not reached by internet signals, a WiMAX connection based on different communication technologies such as WiMAX, Ethernet, GPRS and Satellite, has been set up. PMID:24859030

  5. A new acquisition and imaging system for environmental measurements: an experience on the Italian cultural heritage.

    PubMed

    Leccese, Fabio; Cagnetti, Marco; Calogero, Andrea; Trinca, Daniele; di Pasquale, Stefano; Giarnetti, Sabino; Cozzella, Lorenzo

    2014-05-23

    A new acquisition system for remote control of wall paintings has been realized and tested in the field. The system measures temperature and atmospheric pressure in an archeological site where a fresco has been put under control. The measuring chain has been designed to be used in unfavorable environments where neither electric power nor telecommunication infrastructures are available. The environmental parameters obtained from the local monitoring are then transferred remotely allowing an easier management by experts in the field of conservation of cultural heritage. The local acquisition system uses an electronic card based on microcontrollers and sends the data to a central unit realized with a Raspberry-Pi. The latter manages a high quality camera to pick up pictures of the fresco. Finally, to realize the remote control at a site not reached by internet signals, a WiMAX connection based on different communication technologies such as WiMAX, Ethernet, GPRS and Satellite, has been set up.

  6. Multifunctional data acquisition and analysis and optical sensors: a Bonneville Power Administration (BPA) update

    NASA Astrophysics Data System (ADS)

    Erickson, Dennis C.; Donnelly, Matt K.

    1995-04-01

    The authors present a design concept describing a multifunctional data acquisition and analysis architecture for advanced power system monitoring. The system is tailored to take advantage of the salient features of low energy sensors, particularly optical types. The discussion of the system concept and optical sensors is based on research at BPA and PNL and on progress made at existing BPA installations and other sites in the western power system.

  7. Defense Acquisitions: Assessment of Institute for Defense Analyses C-130 Avionics Modernization Program Analysis

    DTIC Science & Technology

    2014-05-29

    Page 1 GAO-14-547R C-130 Avionics Modernization Program 441 G St. N.W. Washington, DC 20548 May 29, 2014...Congressional Committees Defense Acquisitions: Assessment of Institute for Defense Analyses’ C-130 Avionics Modernization Program Analysis The Air...Force’s C-130 Avionics Modernization Program (AMP), which entered development in 2001, was to standardize and upgrade the cockpit and avionics for

  8. [Design of hand-held heart rate variability acquisition and analysis system].

    PubMed

    Li, Kaiyuan; Wang, Buqing; Wang, Weidong

    2012-07-01

    A design of handheld heart rate variability acquisition and analysis system is proposed. The system collects and stores the patient's ECG every five minutes through both hands touching on the electrodes, and then -uploads data to a PC through USB port. The system uses software written in LabVIEW to analyze heart rate variability parameters, The parameters calculated function is programmed and generated to components in Matlab.

  9. Engineering the Business of Defense Acquisition: An Analysis of Program Office Processes

    DTIC Science & Technology

    2015-05-01

    Engineering the Business of Defense Acquisition: An Analysis of Program Office Processes Charles K. Pickar, Naval Postgraduate School Raymond D...control number. 1. REPORT DATE MAY 2015 2. REPORT TYPE 3. DATES COVERED 00-00-2015 to 00-00-2015 4. TITLE AND SUBTITLE Engineering the Business ...Information Technology and Business Process Redesign | MIT Sloan Management Review. MIT Sloan Management Review. Retrieved from http://sloanreview.mit.edu

  10. Development of an undergraduate optics laboratory based on the analysis of digital images

    NASA Astrophysics Data System (ADS)

    Ramil, Alberto; López, Ana J.; Fiorucci, M. Paula; Vincitorio, Fabio

    2014-07-01

    The aim of this work is to present an experience based on the use of digital images and computer processing techniques for enhanced optics laboratory teaching aids. The use of digital images offers the possibility of analysing some phenomena quantitatively, which would be very difficult to do with the traditional equipment available in teaching labs. In order to obtain high quality teaching material, a number of practical aspects should be taken into account during the process of image acquisition and subsequent analysis. Examples of quantitative experiments are presented; they cover the usual topics at undergraduate level, both geometrical and physical optics and even spectral analysis of the light.

  11. Description, Recognition and Analysis of Biological Images

    SciTech Connect

    Yu Donggang; Jin, Jesse S.; Luo Suhuai; Pham, Tuan D.; Lai Wei

    2010-01-25

    Description, recognition and analysis biological images plays an important role for human to describe and understand the related biological information. The color images are separated by color reduction. A new and efficient linearization algorithm is introduced based on some criteria of difference chain code. A series of critical points is got based on the linearized lines. The series of curvature angle, linearity, maximum linearity, convexity, concavity and bend angle of linearized lines are calculated from the starting line to the end line along all smoothed contours. The useful method can be used for shape description and recognition. The analysis, decision, classification of the biological images are based on the description of morphological structures, color information and prior knowledge, which are associated each other. The efficiency of the algorithms is described based on two applications. One application is the description, recognition and analysis of color flower images. Another one is related to the dynamic description, recognition and analysis of cell-cycle images.

  12. Fast Acquisition and Reconstruction of Optical Coherence Tomography Images via Sparse Representation

    PubMed Central

    Li, Shutao; McNabb, Ryan P.; Nie, Qing; Kuo, Anthony N.; Toth, Cynthia A.; Izatt, Joseph A.; Farsiu, Sina

    2014-01-01

    In this paper, we present a novel technique, based on compressive sensing principles, for reconstruction and enhancement of multi-dimensional image data. Our method is a major improvement and generalization of the multi-scale sparsity based tomographic denoising (MSBTD) algorithm we recently introduced for reducing speckle noise. Our new technique exhibits several advantages over MSBTD, including its capability to simultaneously reduce noise and interpolate missing data. Unlike MSBTD, our new method does not require an a priori high-quality image from the target imaging subject and thus offers the potential to shorten clinical imaging sessions. This novel image restoration method, which we termed sparsity based simultaneous denoising and interpolation (SBSDI), utilizes sparse representation dictionaries constructed from previously collected datasets. We tested the SBSDI algorithm on retinal spectral domain optical coherence tomography images captured in the clinic. Experiments showed that the SBSDI algorithm qualitatively and quantitatively outperforms other state-of-the-art methods. PMID:23846467

  13. Automated grid handling and image acquisition for two-dimensional crystal screening.

    PubMed

    Cheng, Anchi

    2013-01-01

    Routine large-scale two-dimensional (2D) crystallization trials are good candidates for automation. For imaging the large number of grids prepared from 2D crystallization trials, a two-pass imaging protocol using Leginon and a grid handling robot is described. A manual target selection bridges the two imaging passes. The two passes can be combined into one if objects of interests at different stages of trials can be reliably found using automated methods.

  14. Optical Analysis of Microscope Images

    NASA Astrophysics Data System (ADS)

    Biles, Jonathan R.

    Microscope images were analyzed with coherent and incoherent light using analog optical techniques. These techniques were found to be useful for analyzing large numbers of nonsymbolic, statistical microscope images. In the first part phase coherent transparencies having 20-100 human multiple myeloma nuclei were simultaneously photographed at 100 power magnification using high resolution holographic film developed to high contrast. An optical transform was obtained by focussing the laser onto each nuclear image and allowing the diffracted light to propagate onto a one dimensional photosensor array. This method reduced the data to the position of the first two intensity minima and the intensity of successive maxima. These values were utilized to estimate the four most important cancer detection clues of nuclear size, shape, darkness, and chromatin texture. In the second part, the geometric and holographic methods of phase incoherent optical processing were investigated for pattern recognition of real-time, diffuse microscope images. The theory and implementation of these processors was discussed in view of their mutual problems of dimness, image bias, and detector resolution. The dimness problem was solved by either using a holographic correlator or a speckle free laser microscope. The latter was built using a spinning tilted mirror which caused the speckle to change so quickly that it averaged out during the exposure. To solve the bias problem low image bias templates were generated by four techniques: microphotography of samples, creation of typical shapes by computer graphics editor, transmission holography of photoplates of samples, and by spatially coherent color image bias removal. The first of these templates was used to perform correlations with bacteria images. The aperture bias was successfully removed from the correlation with a video frame subtractor. To overcome the limited detector resolution it is necessary to discover some analog nonlinear intensity

  15. System and method for improving ultrasound image acquisition and replication for repeatable measurements of vascular structures

    NASA Technical Reports Server (NTRS)

    Selzer, Robert H. (Inventor); Hodis, Howard N. (Inventor)

    2006-01-01

    High resolution B-mode ultrasound images of the common carotid artery are obtained with an ultrasound transducer using a standardized methodology. Subjects are supine with the head counter-rotated 45 degrees using a head pillow. The jugular vein and carotid artery are located and positioned in a vertical stacked orientation. The transducer is rotated 90 degrees around the centerline of the transverse image of the stacked structure to obtain a longitudinal image while maintaining the vessels in a stacked position. A computerized methodology assists operators to accurately replicate images obtained over several spaced-apart examinations. The methodology utilizes a split-screen display in which the arterial ultrasound image from an earlier examination is displayed on one side of the screen while a real-time live ultrasound image from a current examination is displayed next to the earlier image on the opposite side of the screen. By viewing both images, whether simultaneously or alternately, while manually adjusting the ultrasound transducer, an operator is able to bring into view the real-time image that best matches a selected image from the earlier ultrasound examination. Utilizing this methodology, measurement of vascular dimensions such as carotid arterial IMT and diameter, the coefficient of variation is substantially reduced to values approximating from about 1.0% to about 1.25%. All images contain anatomical landmarks for reproducing probe angulation, including visualization of the carotid bulb, stacking of the jugular vein above the carotid artery, and initial instrumentation settings, used at a baseline measurement are maintained during all follow-up examinations.

  16. Imaging flow cytometry for phytoplankton analysis.

    PubMed

    Dashkova, Veronika; Malashenkov, Dmitry; Poulton, Nicole; Vorobjev, Ivan; Barteneva, Natasha S

    2017-01-01

    This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities' composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments.

  17. Digital Image Analysis for DETCHIP® Code Determination

    PubMed Central

    Lyon, Marcus; Wilson, Mark V.; Rouhier, Kerry A.; Symonsbergen, David J.; Bastola, Kiran; Thapa, Ishwor; Holmes, Andrea E.

    2013-01-01

    DETECHIP® is a molecular sensing array used for identification of a large variety of substances. Previous methodology for the analysis of DETECHIP® used human vision to distinguish color changes induced by the presence of the analyte of interest. This paper describes several analysis techniques using digital images of DETECHIP®. Both a digital camera and flatbed desktop photo scanner were used to obtain Jpeg images. Color information within these digital images was obtained through the measurement of red-green-blue (RGB) values using software such as GIMP, Photoshop and ImageJ. Several different techniques were used to evaluate these color changes. It was determined that the flatbed scanner produced in the clearest and more reproducible images. Furthermore, codes obtained using a macro written for use within ImageJ showed improved consistency versus pervious methods. PMID:25267940

  18. Digital live–tracking 3–dimensional minisensors for recording head orientation during image acquisition

    PubMed Central

    de Paula, Leonardo Koerich; Ackerman, James L.; Carvalho, Felipe de Assis Ribeiro; Eidson, Lindsey; Cevidanes, Lucia Helena Soares

    2013-01-01

    Introduction Our objective was to test the value of minisensors for recording unrestrained head position with 6 degrees of freedom during 3-dimensional stereophotogrammetry. Methods Four 3-dimensional pictures (3dMD, Atlanta, Ga) were taken of 20 volunteers as follows: (1) in unrestrained head position, (2) a repeat of picture 1, (3) in unrestrained head position wearing a headset with 3-dimensional live tracking sensors (3-D Guidance trackSTAR; Ascension Technology, Burlington, Vt), and (4) a repeat of picture 3. The sensors were used to track the x, y, and z coordinates (pitch, roll, and yaw) of the head in space. The patients were seated in front of a mirror and asked to stand and take a walk between each acquisition. Eight landmarks were identified in each 3-dimensional picture (nasion, tip of nose, subnasale, right and left lip commissures, midpoints of upper and lower lip vermilions, soft-tissue B-point). The distances between correspondent landmarks were measured between pictures 1 and 2 and 3 and 4 with software. The Student t test was used to test differences between unrestrained head position with and without sensors. Results Interlandmark distances for pictures 1 and 2 (head position without the sensors) and pictures 3 and 4 (head position with sensors) were consistent for all landmarks, indicating that roll, pitch, and yaw of the head are controlled independently of the sensors. However, interlandmark distances were on average 17.34 ± 0.32 mm between pictures 1 and 2. Between pictures 3 and 4, the distances averaged 6.17 ± 0.15 mm. All interlandmark distances were significantly different between the 2 methods (P<0.001). Conclusions The use of 3-dimensional live-tracking sensors aids the reproducibility of patient head positioning during repeated or follow-up acquisitions of 3-dimensional stereophotogrammetry. Even with sensors, differences in spatial head position between acquisitions still require additional registration procedures. PMID:22196193

  19. Integrated Colony Imaging, Analysis, and Selection Device for Regenerative Medicine.

    PubMed

    Kwee, Edward; Herderick, Edward E; Adams, Thomas; Dunn, James; Germanowski, Robert; Krakosh, Frank; Boehm, Cynthia; Monnich, James; Powell, Kimerly; Muschler, George

    2017-04-01

    Stem and progenitor cells derived from human tissues are being developed as cell sources for cell-based assays and therapies. However, tissue-derived stem and progenitor cells are heterogeneous. Differences in observed clones of stem cells likely reflect important aspects of the underlying state of the source cells, as well as future potency for cell therapies. This paper describes a colony analysis and picking device that provides quantitative analysis of heterogeneous cell populations and precise tools for cell picking for research or biomanufacturing applications. We describe an integrated robotic system that enables image acquisition and automated image analysis to be coupled with rapid automated selection of individual colonies in adherent cell cultures. Other automated systems have demonstrated feasibility with picking from semisolid media or off feeder layers. We demonstrate the capability to pick adherent bone-derived stem cells from tissue culture plastic. Cells are efficiently picked from a target site and transferred to a recipient well plate. Cells demonstrate viability and adherence and maintain biologic potential for surface markers CD73 and CD90 based on phase contrast and fluorescence imaging 6 days after transfer. Methods developed here can be applied to the study of other stem cell types and automated culture of cells.

  20. Development of filter exchangeable 3CCD camera for multispectral imaging acquisition

    NASA Astrophysics Data System (ADS)

    Lee, Hoyoung; Park, Soo Hyun; Kim, Moon S.; Noh, Sang Ha

    2012-05-01

    There are a lot of methods to acquire multispectral images. Dynamic band selective and area-scan multispectral camera has not developed yet. This research focused on development of a filter exchangeable 3CCD camera which is modified from the conventional 3CCD camera. The camera consists of F-mounted lens, image splitter without dichroic coating, three bandpass filters, three image sensors, filer exchangeable frame and electric circuit for parallel image signal processing. In addition firmware and application software have developed. Remarkable improvements compared to a conventional 3CCD camera are its redesigned image splitter and filter exchangeable frame. Computer simulation is required to visualize a pathway of ray inside of prism when redesigning image splitter. Then the dimensions of splitter are determined by computer simulation which has options of BK7 glass and non-dichroic coating. These properties have been considered to obtain full wavelength rays on all film planes. The image splitter is verified by two line lasers with narrow waveband. The filter exchangeable frame is designed to make swap bandpass filters without displacement change of image sensors on film plane. The developed 3CCD camera is evaluated to application of detection to scab and bruise on Fuji apple. As a result, filter exchangeable 3CCD camera could give meaningful functionality for various multispectral applications which need to exchange bandpass filter.

  1. [The acquisition and analysis of heart sound signals based on DSP].

    PubMed

    Xing, Suxia; Chen, Tianhua

    2011-04-01

    Heart sound signals acquisition is the primary basis for achieving non-invasive diagnosis of coronary heart disease. In this paper, a digital signal processor (DSP)-based on miniaturized circuit of heart sound signals acquisition and analysis platform was designed to achieve the functions of filtering, collecting, processing, displaying and the communicating with PC. With the self-developed experimental platform, we collected 228 cases of heart sounds of clinical data, and processed the signals using de-noising method with wavelet transform. These experimental results indicated that the db6 wavelet has the most obvious de-noising effect among the four most commonly used wavelets, i.e., haar, db6, sym8, and coif5. One wavelet at different levels possessed different de-noising effects, with level-5 db6 decomposition obtaining the most desirable result.

  2. Video-task acquisition in rhesus monkeys (Macaca mulatta) and chimpanzees (Pan troglodytes): a comparative analysis

    NASA Technical Reports Server (NTRS)

    Hopkins, W. D.; Washburn, D. A.; Hyatt, C. W.; Rumbaugh, D. M. (Principal Investigator)

    1996-01-01

    This study describes video-task acquisition in two nonhuman primate species. The subjects were seven rhesus monkeys (Macaca mulatta) and seven chimpanzees (Pan troglodytes). All subjects were trained to manipulate a joystick which controlled a cursor displayed on a computer monitor. Two criterion levels were used: one based on conceptual knowledge of the task and one based on motor performance. Chimpanzees and rhesus monkeys attained criterion in a comparable number of trials using a conceptually based criterion. However, using a criterion based on motor performance, chimpanzees reached criterion significantly faster than rhesus monkeys. Analysis of error patterns and latency indicated that the rhesus monkeys had a larger asymmetry in response bias and were significantly slower in responding than the chimpanzees. The results are discussed in terms of the relation between object manipulation skills and video-task acquisition.

  3. Materials characterization through quantitative digital image analysis

    SciTech Connect

    J. Philliber; B. Antoun; B. Somerday; N. Yang

    2000-07-01

    A digital image analysis system has been developed to allow advanced quantitative measurement of microstructural features. This capability is maintained as part of the microscopy facility at Sandia, Livermore. The system records images digitally, eliminating the use of film. Images obtained from other sources may also be imported into the system. Subsequent digital image processing enhances image appearance through the contrast and brightness adjustments. The system measures a variety of user-defined microstructural features--including area fraction, particle size and spatial distributions, grain sizes and orientations of elongated particles. These measurements are made in a semi-automatic mode through the use of macro programs and a computer controlled translation stage. A routine has been developed to create large montages of 50+ separate images. Individual image frames are matched to the nearest pixel to create seamless montages. Results from three different studies are presented to illustrate the capabilities of the system.

  4. An image acquisition and registration strategy for the fusion of hyperpolarized helium-3 MRI and x-ray CT images of the lung

    NASA Astrophysics Data System (ADS)

    Ireland, Rob H.; Woodhouse, Neil; Hoggard, Nigel; Swinscoe, James A.; Foran, Bernadette H.; Hatton, Matthew Q.; Wild, Jim M.

    2008-11-01

    The purpose of this ethics committee approved prospective study was to evaluate an image acquisition and registration protocol for hyperpolarized helium-3 magnetic resonance imaging (3He-MRI) and x-ray computed tomography. Nine patients with non-small cell lung cancer (NSCLC) gave written informed consent to undergo a free-breathing CT, an inspiration breath-hold CT and a 3D ventilation 3He-MRI in CT position using an elliptical birdcage radiofrequency (RF) body coil. 3He-MRI to CT image fusion was performed using a rigid registration algorithm which was assessed by two observers using anatomical landmarks and a percentage volume overlap coefficient. Registration of 3He-MRI to breath-hold CT was more accurate than to free-breathing CT; overlap 82.9 ± 4.2% versus 59.8 ± 9.0% (p < 0.001) and mean landmark error 0.75 ± 0.24 cm versus 1.25 ± 0.60 cm (p = 0.002). Image registration is significantly improved by using an imaging protocol that enables both 3He-MRI and CT to be acquired with similar breath holds and body position through the use of a birdcage 3He-MRI body RF coil and an inspiration breath-hold CT. Fusion of 3He-MRI to CT may be useful for the assessment of patients with lung diseases.

  5. Diffusion-weighted imaging in the prostate: an apparent diffusion coefficient comparison of half-Fourier acquisition single-shot turbo spin-echo and echo planar imaging.

    PubMed

    Babourina-Brooks, Ben; Cowin, Gary J; Wang, Deming

    2012-02-01

    Prostate cancer detection using diffusion-weighted imaging is highly affected by the accuracy of the apparent diffusion coefficient (ADC) values in an image. Echo planar imaging (EPI) is a fast sequence commonly used for diffusion imaging but has inherent magnetic susceptibility and chemical shift artefacts associated. A diffusion sequence that is less affected by these artefacts is therefore advantageous. The half-Fourier acquisition single-shot turbo spin-echo (HASTE) sequence was chosen. The diffusion sequences were compared in image quality, repeatability of the ADC value and the effect on the ADC value with varied b values. Eight volunteers underwent three scans of each sequence, on a 1.5-T Siemens system, using b values of 0, 150, 300, 450, 600, 750, 900 and 1000 s/mm(2). ADC maps were created to address the reproducibility of the ADC value when using two b values compared to eight b values. The ADC value using all b values with the HASTE sequence gave the best performance in all tested categories. Both sequences gave significantly different ADC mean values for two b values compared to when using eight b values (P<.05) suggesting larger error is present when using two b values. HASTE was shown to be an improvement over EPI in terms of repeatability, signal variation within a region of interest and standard deviation over the volunteer set. The improved accuracy of the ADC value in the HASTE sequence makes it potentially a more sensitive tumor detection technique.

  6. Imaging System and Method for Biomedical Analysis

    DTIC Science & Technology

    2013-03-11

    compressive decoding of sparse objects” by A. Coskum et al. 136 Analyst No. 17, pp. 3512–3518, (7 September 2011). Their fluorescent microscopy lensless ...prior art concept is a lensless imaging system proposed in a research paper entitled “ Lensless wide-field fluorescent imaging on a chip using...analysis. [0008] An article published by Sang Jun Moon et al., “Integrating Micro-fluidics and Lensless Imaging for Point-of- Care,” 24 Biosens

  7. Theory of Image Analysis and Recognition.

    DTIC Science & Technology

    1983-01-24

    Narendra Ahuja Image models Ramalingam Chellappa Image models Matti Pietikainen * Texture analysis b David G. Morgenthaler’ 3D digital geometry c Angela Y. Wu...Restoration Parameter Choice A Quantitative Guide," TR-965, October 1980. 70. Matti Pietikainen , "On the Use of Hierarchically Computed ’Mexican Hat...81. Matti Pietikainen and Azriel Rosenfeld, "Image Segmenta- tion by Texture Using Pyramid Node Linking," TR-1008, February 1981. 82. David G. 1

  8. Analysis of dynamic brain imaging data.

    PubMed Central

    Mitra, P P; Pesaran, B

    1999-01-01

    Modern imaging techniques for probing brain function, including functional magnetic resonance imaging, intrinsic and extrinsic contrast optical imaging, and magnetoencephalography, generate large data sets with complex content. In this paper we develop appropriate techniques for analysis and visualization of such imaging data to separate the signal from the noise and characterize the signal. The techniques developed fall into the general category of multivariate time series analysis, and in particular we extensively use the multitaper framework of spectral analysis. We develop specific protocols for the analysis of fMRI, optical imaging, and MEG data, and illustrate the techniques by applications to real data sets generated by these imaging modalities. In general, the analysis protocols involve two distinct stages: "noise" characterization and suppression, and "signal" characterization and visualization. An important general conclusion of our study is the utility of a frequency-based representation, with short, moving analysis windows to account for nonstationarity in the data. Of particular note are 1) the development of a decomposition technique (space-frequency singular value decomposition) that is shown to be a useful means of characterizing the image data, and 2) the development of an algorithm, based on multitaper methods, for the removal of approximately periodic physiological artifacts arising from cardiac and respiratory sources. PMID:9929474

  9. Reducing Contrast Contamination in Radial Turbo-Spin-Echo Acquisitions by Combining a Narrow-Band KWIC Filter With Parallel Imaging

    PubMed Central

    Neumann, Daniel; Breuer, Felix A.; Völker, Michael; Brandt, Tobias; Griswold, Mark A.; Jakob, Peter M.; Blaimer, Martin

    2014-01-01

    Purpose Cartesian turbo spin-echo (TSE) and radial TSE images are usually reconstructed by assembling data containing different contrast information into a single k-space. This approach results in mixed contrast contributions in the images, which may reduce their diagnostic value. The goal of this work is to improve the image contrast from radial TSE acquisitions by reducing the contribution of signals with undesired contrast information. Methods Radial TSE acquisitions allow the reconstruction of multiple images with different T2 contrasts using the k-space weighted image contrast (KWIC) filter. In this work, the image contrast is improved by reducing the band-width of the KWIC filter. Data for the reconstruction of a single image are selected from within a small temporal range around the desired echo time. The resulting data set is undersampled and therefore an iterative parallel imaging algorithm is applied to remove aliasing artifacts. Results Radial TSE images of the human brain reconstructed with the proposed method show an improved contrast when compared to Cartesian TSE images or radial TSE images with conventional KWIC reconstructions. Conclusion The proposed method provides multi-contrast images from radial TSE data with contrasts similar to multi spin-echo images. Contaminations from unwanted contrast weightings are strongly reduced. PMID:24436227

  10. Cone-Beam Computed Tomography: Imaging Dose during CBCT Scan Acquisition and Accuracy of CBCT Based Dose Calculations

    NASA Astrophysics Data System (ADS)

    Giles, David Matthew

    Cone beam computed tomography (CBCT) is a recent development in radiotherapy for use in image guidance. Image guided radiotherapy using CBCT allows visualization of soft tissue targets and critical structures prior to treatment. Dose escalation is made possible by accurately localizing the target volume while reducing normal tissue toxicity. The kilovoltage x-rays of the cone beam imaging system contribute additional dose to the patient. In this study a 2D reference radiochromic film dosimetry method employing GAFCHROMIC(TM) model XR-QA film is used to measure point skin doses and dose profiles from the Elekta XVI CBCT system integrated onto the Synergy linac. The soft tissue contrast of the daily CBCT images makes adaptive radiotherapy possible in the clinic. In order to track dose to the patient or utilize on-line replanning for adaptive radiotherapy the CBCT images must be used to calculate dose. A Hounsfield unit calibration method for scatter correction is investigated for heterogeneity corrected dose calculation in CBCT images. Three Hounsfield unit to density calibration tables are used for each of four cases including patients and an anthropomorphic phantom, and the calculated dose from each is compared to results from the clinical standard fan beam CT. The dose from the scan acquisition is reported and the effect of scan geometry and total output of the x-ray tube on dose magnitude and distribution is shown. The ability to calculate dose with CBCT is shown to improve with the use of patient specific density tables for scatter correction, and for high beam energies the calculated dose agreement is within 1%.

  11. Imaging MS Methodology for More Chemical Information in Less Data Acquisition Time Utilizing a Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer

    SciTech Connect

    Perdian, D. C.; Lee, Young Jin

    2010-11-15

    A novel mass spectrometric imaging method is developed to reduce the data acquisition time and provide rich chemical information using a hybrid linear ion trap-orbitrap mass spectrometer. In this method, the linear ion trap and orbitrap are used in tandem to reduce the acquisition time by incorporating multiple linear ion trap scans during an orbitrap scan utilizing a spiral raster step plate movement. The data acquisition time was decreased by 43-49% in the current experiment compared to that of orbitrap-only scans; however, 75% or more time could be saved for higher mass resolution and with a higher repetition rate laser. Using this approach, a high spatial resolution of 10 {micro}m was maintained at ion trap imaging, while orbitrap spectra were acquired at a lower spatial resolution, 20-40 {micro}m, all with far less data acquisition time. Furthermore, various MS imaging methods were developed by interspersing MS/MS and MSn ion trap scans during orbitrap scans to provide more analytical information on the sample. This method was applied to differentiate and localize structural isomers of several flavonol glycosides from an Arabidopsis flower petal in which MS/MS, MSn, ion trap, and orbitrap images were all acquired in a single data acquisition.

  12. Digital image processing in cephalometric analysis.

    PubMed

    Jäger, A; Döler, W; Schormann, T

    1989-01-01

    Digital image processing methods were applied to improve the practicability of cephalometric analysis. The individual X-ray film was digitized by the aid of a high resolution microscope-photometer. Digital processing was done using a VAX 8600 computer system. An improvement of the image quality was achieved by means of various digital enhancement and filtering techniques.

  13. Technique for real-time frontal face image acquisition using stereo system

    NASA Astrophysics Data System (ADS)

    Knyaz, Vladimir A.; Vizilter, Yuri V.; Kudryashov, Yuri I.

    2013-04-01

    Most part of existing systems for face recognition is usually based on two-dimensional images. And the quality of recognition is rather high for frontal images of face. But for other kind of images the quality decreases significantly. It is necessary to compensate for the effect of a change in the posture of a person (the camera angle) for correct operation of such systems. There are methods of transformation of 2D image of the person to the canonical orientation. The efficiency of these methods depends on the accuracy of determination of specific anthropometric points. Problems can arise for cases of partly occlusion of the person`s face. Another approach is to have a set of person images for different view angles for the further processing. But a need for storing and processing a large number of two-dimensional images makes this method considerably time-consuming. The proposed technique uses stereo system for fast generation of person face 3D model and obtaining face image in given orientation using this 3D model. Real-time performance is provided by implementing and graph cut methods for face surface 3D reconstruction and applying CUDA software library for parallel calculation.

  14. Mapping Fire Severity Using Imaging Spectroscopy and Kernel Based Image Analysis

    NASA Astrophysics Data System (ADS)

    Prasad, S.; Cui, M.; Zhang, Y.; Veraverbeke, S.

    2014-12-01

    Improved spatial representation of within-burn heterogeneity after wildfires is paramount to effective land management decisions and more accurate fire emissions estimates. In this work, we demonstrate feasibility and efficacy of airborne imaging spectroscopy (hyperspectral imagery) for quantifying wildfire burn severity, using kernel based image analysis techniques. Two different airborne hyperspectral datasets, acquired over the 2011 Canyon and 2013 Rim fire in California using the Airborne Visible InfraRed Imaging Spectrometer (AVIRIS) sensor, were used in this study. The Rim Fire, covering parts of the Yosemite National Park started on August 17, 2013, and was the third largest fire in California's history. Canyon Fire occurred in the Tehachapi mountains, and started on September 4, 2011. In addition to post-fire data for both fires, half of the Rim fire was also covered with pre-fire images. Fire severity was measured in the field using Geo Composite Burn Index (GeoCBI). The field data was utilized to train and validate our models, wherein the trained models, in conjunction with imaging spectroscopy data were used for GeoCBI estimation wide geographical regions. This work presents an approach for using remotely sensed imagery combined with GeoCBI field data to map fire scars based on a non-linear (kernel based) epsilon-Support Vector Regression (e-SVR), which was used to learn the relationship between spectra and GeoCBI in a kernel-induced feature space. Classification of healthy vegetation versus fire-affected areas based on morphological multi-attribute profiles was also studied. The availability of pre- and post-fire imaging spectroscopy data over the Rim Fire provided a unique opportunity to evaluate the performance of bi-temporal imaging spectroscopy for assessing post-fire effects. This type of data is currently constrained because of limited airborne acquisitions before a fire, but will become widespread with future spaceborne sensors such as those on

  15. Machine learning applications in cell image analysis.

    PubMed

    Kan, Andrey

    2017-04-04

    Machine learning (ML) refers to a set of automatic pattern recognition methods that have been successfully applied across various problem domains, including biomedical image analysis. This review focuses on ML applications for image analysis in light microscopy experiments with typical tasks of segmenting and tracking individual cells, and modelling of reconstructed lineage trees. After describing a typical image analysis pipeline and highlighting challenges of automatic analysis (for example, variability in cell morphology, tracking in presence of clutters) this review gives a brief historical outlook of ML, followed by basic concepts and definitions required for understanding examples. This article then presents several example applications at various image processing stages, including the use of supervised learning methods for improving cell segmentation, and the application of active learning for tracking. The review concludes with remarks on parameter setting and future directions.Immunology and Cell Biology advance online publication, 4 April 2017; doi:10.1038/icb.2017.16.

  16. A Robust Actin Filaments Image Analysis Framework

    PubMed Central

    Alioscha-Perez, Mitchel; Benadiba, Carine; Goossens, Katty; Kasas, Sandor; Dietler, Giovanni; Willaert, Ronnie; Sahli, Hichem

    2016-01-01

    The cytoskeleton is a highly dynamical protein network that plays a central role in numerous cellular physiological processes, and is traditionally divided into three components according to its chemical composition, i.e. actin, tubulin and intermediate filament cytoskeletons. Understanding the cytoskeleton dynamics is of prime importance to unveil mechanisms involved in cell adaptation to any stress type. Fluorescence imaging of cytoskeleton structures allows analyzing the impact of mechanical stimulation in the cytoskeleton, but it also imposes additional challenges in the image processing stage, such as the presence of imaging-related artifacts and heavy blurring introduced by (high-throughput) automated scans. However, although there exists a considerable number of image-based analytical tools to address the image processing and analysis, most of them are unfit to cope with the aforementioned challenges. Filamentous structures in images can be considered as a piecewise composition of quasi-straight segments (at least in some finer or coarser scale). Based on this observation, we propose a three-steps actin filaments extraction methodology: (i) first the input image is decomposed into a ‘cartoon’ part corresponding to the filament structures in the image, and a noise/texture part, (ii) on the ‘cartoon’ image, we apply a multi-scale line detector coupled with a (iii) quasi-straight filaments merging algorithm for fiber extraction. The proposed robust actin filaments image analysis framework allows extracting individual filaments in the presence of noise, artifacts and heavy blurring. Moreover, it provides numerous parameters such as filaments orientation, position and length, useful for further analysis. Cell image decomposition is relatively under-exploited in biological images processing, and our study shows the benefits it provides when addressing such tasks. Experimental validation was conducted using publicly available datasets, and in osteoblasts

  17. Acquisition, Tracking, and Pointing Using Earth Thermal Images for Deep Space Optical Communications

    NASA Technical Reports Server (NTRS)

    Ortiz, Gerry G.; Lee, Shinhak

    2003-01-01

    The feasibility of using long wavelength Earth thermal (infrared) images for telescope tracking/pointing application. for both Deep Space Free- pace Optical Communications has been investigated and is reported her. The advantage of this technology rests on using full Earth images in this band, which yield more accurate estimates of geometric centroids than that of Earth images in the visible band. Another major advantage is that these images are nearly independent of Earth phase angle. The results of the study show that at a Mars range, with currently available sensors, a noise equivalent angle of 10 to 150 nanoradians and a bias error of better than 80 nanoradians can be obtained. This enables precise pointing of the optical communications beam for high data rate links.

  18. The design and validation of a magnetic resonance imaging-compatible device for obtaining mechanical properties of plantar soft tissue via gated acquisition.

    PubMed

    Williams, Evan D; Stebbins, Michael J; Cavanagh, Peter R; Haynor, David R; Chu, Baocheng; Fassbind, Michael J; Isvilanonda, Vara; Ledoux, William R

    2015-10-01

    Changes in the mechanical properties of the plantar soft tissue in people with diabetes may contribute to the formation of plantar ulcers. Such ulcers have been shown to be in the causal pathway for lower extremity amputation. The hydraulic plantar soft tissue reducer (HyPSTER) was designed to measure in vivo, rate-dependent plantar soft tissue compressive force and three-dimensional deformations to help understand, predict, and prevent ulcer formation. These patient-specific values can then be used in an inverse finite element analysis to determine tissue moduli, and subsequently used in a foot model to show regions of high stress under a wide variety of loading conditions. The HyPSTER uses an actuator to drive a magnetic resonance imaging-compatible hydraulic loading platform. Pressure and actuator position were synchronized with gated magnetic resonance imaging acquisition. Achievable loading rates were slower than those found in normal walking because of a water-hammer effect (pressure wave ringing) in the hydraulic system when the actuator direction was changed rapidly. The subsequent verification tests were, therefore, performed at 0.2 Hz. The unloaded displacement accuracy of the system was within 0.31%. Compliance, presumably in the system's plastic components, caused a displacement loss of 5.7 mm during a 20-mm actuator test at 1354 N. This was accounted for with a target to actual calibration curve. The positional accuracy of the HyPSTER during loaded displacement verification tests from 3 to 9 mm against a silicone backstop was 95.9% with a precision of 98.7%. The HyPSTER generated minimal artifact in the magnetic resonance imaging scanner. Careful analysis of the synchronization of the HyPSTER and the magnetic resonance imaging scanner was performed. With some limitations, the HyPSTER provided key functionality in measuring dynamic, patient-specific plantar soft tissue mechanical properties.

  19. On image analysis in fractography (Methodological Notes)

    NASA Astrophysics Data System (ADS)

    Shtremel', M. A.

    2015-10-01

    As other spheres of image analysis, fractography has no universal method for information convolution. An effective characteristic of an image is found by analyzing the essence and origin of every class of objects. As follows from the geometric definition of a fractal curve, its projection onto any straight line covers a certain segment many times; therefore, neither a time series (one-valued function of time) nor an image (one-valued function of plane) can be a fractal. For applications, multidimensional multiscale characteristics of an image are necessary. "Full" wavelet series break the law of conservation of information.

  20. Morphology enabled dipole inversion (MEDI) from a single-angle acquisition: comparison with COSMOS in human brain imaging.

    PubMed

    Liu, Tian; Liu, Jing; de Rochefort, Ludovic; Spincemaille, Pascal; Khalidov, Ildar; Ledoux, James Robert; Wang, Yi

    2011-09-01

    Magnetic susceptibility varies among brain structures and provides insights into the chemical and molecular composition of brain tissues. However, the determination of an arbitrary susceptibility distribution from the measured MR signal phase is a challenging, ill-conditioned inverse problem. Although a previous method named calculation of susceptibility through multiple orientation sampling (COSMOS) has solved this inverse problem both theoretically and experimentally using multiple angle acquisitions, it is often impractical to carry out on human subjects. Recently, the feasibility of calculating the brain susceptibility distribution from a single-angle acquisition was demonstrated using morphology enabled dipole inversion (MEDI). In this study, we further improved the original MEDI method by sparsifying the edges in the quantitative susceptibility map that do not have a corresponding edge in the magnitude image. Quantitative susceptibility maps generated by the improved MEDI were compared qualitatively and quantitatively with those generated by calculation of susceptibility through multiple orientation sampling. The results show a high degree of agreement between MEDI and calculation of susceptibility through multiple orientation sampling, and the practicality of MEDI allows many potential clinical applications.

  1. Design and implementation of photoelectric rotary table data acquisition and analysis system host computer software based on VC++ and MFC

    NASA Astrophysics Data System (ADS)

    Yang, Dawei; Yang, Xiufang; Han, Junfeng; Yan, Xiaoxu

    2015-02-01

    Photoelectric rotary table is mainly used in the defense industry and military fields, especially in the shooting range, target tracking, target acquisition, aerospace aspects play an important one. For range photoelectric measuring equipment field test application requirements, combined with a portable photoelectric rotary table data acquisition hardware system, software programming platform is presented based on the VC++, using MFC prepared PC interface, the realization of photoelectric turntable data acquisition, analysis and processing and debugging control. The host computer software design of serial communication and protocol, real-time data acquisition and display, real-time data curve drawing, analog acquisition, debugging guide, error analysis program, and gives the specific design method. Finally, through the photoelectric rotary table data acquisition hardware system alignment, the experimental results show that host computer software can better accomplish with lower machine data transmission, data acquisition, control and analysis, and to achieve the desired effect, the entire software system running performance is stable, flexible, strong practicality and reliability, the advantages of good scalability.

  2. Retinal image analysis: concepts, applications and potential.

    PubMed

    Patton, Niall; Aslam, Tariq M; MacGillivray, Thomas; Deary, Ian J; Dhillon, Baljean; Eikelboom, Robert H; Yogesan, Kanagasingam; Constable, Ian J

    2006-01-01

    As digital imaging and computing power increasingly develop, so too does the potential to use these technologies in ophthalmology. Image processing, analysis and computer vision techniques are increasing in prominence in all fields of medical science, and are especially pertinent to modern ophthalmology, as it is heavily dependent on visually oriented signs. The retinal microvasculature is unique in that it is the only part of the human circulation that can be directly visualised non-invasively in vivo, readily photographed and subject to digital image analysis. Exciting developments in image processing relevant to ophthalmology over the past 15 years includes the progress being made towards developing automated diagnostic systems for conditions, such as diabetic retinopathy, age-related macular degeneration and retinopathy of prematurity. These diagnostic systems offer the potential to be used in large-scale screening programs, with the potential for significant resource savings, as well as being free from observer bias and fatigue. In addition, quantitative measurements of retinal vascular topography using digital image analysis from retinal photography have been used as research tools to better understand the relationship between the retinal microvasculature and cardiovascular disease. Furthermore, advances in electronic media transmission increase the relevance of using image processing in 'teleophthalmology' as an aid in clinical decision-making, with particular relevance to large rural-based communities. In this review, we outline the principles upon which retinal digital image analysis is based. We discuss current techniques used to automatically detect landmark features of the fundus, such as the optic disc, fovea and blood vessels. We review the use of image analysis in the automated diagnosis of pathology (with particular reference to diabetic retinopathy). We also review its role in defining and performing quantitative measurements of vascular topography

  3. Edge enhanced morphology for infrared image analysis

    NASA Astrophysics Data System (ADS)

    Bai, Xiangzhi; Liu, Haonan

    2017-01-01

    Edge information is one of the critical information for infrared images. Morphological operators have been widely used for infrared image analysis. However, the edge information in infrared image is weak and the morphological operators could not well utilize the edge information of infrared images. To strengthen the edge information in morphological operators, the edge enhanced morphology is proposed in this paper. Firstly, the edge enhanced dilation and erosion operators are given and analyzed. Secondly, the pseudo operators which are derived from the edge enhanced dilation and erosion operators are defined. Finally, the applications for infrared image analysis are shown to verify the effectiveness of the proposed edge enhanced morphological operators. The proposed edge enhanced morphological operators are useful for the applications related to edge features, which could be extended to wide area of applications.

  4. Efficient imaging of midbrain nuclei using inverse double-echo steady-state acquisition a)

    PubMed Central

    Wu, Ming-Long; Chang, Hing-Chiu; Chao, Tzu-Cheng; Chen, Nan-Kuei

    2015-01-01

    Purpose: Imaging of midbrain nuclei using T2- or T2*-weighted MRI often entails long echo time, leading to long scan time. In this study, an inverse double-echo steady-state (iDESS) technique is proposed for efficiently depicting midbrain nuclei. Methods: Thirteen healthy subjects participated in this study. iDESS was performed along with two sets of T2*-weighted spoiled gradient-echo images (SPGR1, with scan time identical to iDESS and SPGR2, using clinical scanning parameters as a reference standard) for comparison. Generation of iDESS composite images combining two echo signals was optimized for maximal contrast-to-noise ratio (CNR) between the red nuclei and surrounding tissues. Signal-to-noise ratios (SNRs) were calculated from the occipital lobe. Comparison was also made using phase-enhanced images as in standard susceptibility-weighted imaging (SWI). Results: The iDESS images present significantly higher SNR efficiency (171.3) than SPGR1 (158.7, p = 0.013) and SPGR2 (95.5, p < 10−8). iDESS CNR efficiency (19.2) is also significantly greater than SPGR1 (6.9, p < 10−6) and SPGR2 (14.3, p = 0.0016). Compared with DESS, iDESS provides further advantage on enhanced phase information and hence improved contrast on SWI-processed images. Conclusions: iDESS efficiently depicts midbrain nuclei with improved CNR efficiency, increased SNR efficiency, and reduced scan time and is less prone to susceptibility signal loss from air-tissue interfaces. PMID:26133633

  5. Diagnosing Lung Nodules on Oncologic MR/PET Imaging: Comparison of Fast T1-Weighted Sequences and Influence of Image Acquisition in Inspiration and Expiration Breath-Hold

    PubMed Central

    Schwenzer, Nina F.; Seith, Ferdinand; Gatidis, Sergios; Brendle, Cornelia; Schmidt, Holger; Pfannenberg, Christina A.; laFougère, Christian; Nikolaou, Konstantin

    2016-01-01

    Objective First, to investigate the diagnostic performance of fast T1-weighted sequences for lung nodule evaluation in oncologic magnetic resonance (MR)/positron emission tomography (PET). Second, to evaluate the influence of image acquisition in inspiration and expiration breath-hold on diagnostic performance. Materials and Methods The study was approved by the local Institutional Review Board. PET/CT and MR/PET of 44 cancer patients were evaluated by 2 readers. PET/CT included lung computed tomography (CT) scans in inspiration and expiration (CTin, CTex). MR/PET included Dixon sequence for attenuation correction and fast T1-weighted volumetric interpolated breath-hold examination (VIBE) sequences (volume interpolated breath-hold examination acquired in inspiration [VIBEin], volume interpolated breath-hold examination acquired in expiration [VIBEex]). Diagnostic performance was analyzed for lesion-, lobe-, and size-dependence. Diagnostic confidence was evaluated (4-point Likert-scale; 1 = high). Jackknife alternative free-response receiver-operating characteristic (JAFROC) analysis was performed. Results Seventy-six pulmonary lesions were evaluated. Lesion-based detection rates were: CTex, 77.6%; VIBEin, 53.3%; VIBEex, 51.3%; and Dixon, 22.4%. Lobe-based detection rates were: CTex, 89.6%; VIBEin, 58.3%; VIBEex, 60.4%; and Dixon, 31.3%. In contrast to CT, inspiration versus expiration did not alter diagnostic performance in VIBE sequences. Diagnostic confidence was best for VIBEin and CTex and decreased in VIBEex and Dixon (1.2 ± 0.6; 1.2 ± 0.7; 1.5 ± 0.9; 1.7 ± 1.1, respectively). The JAFROC figure-of-merit of Dixon was significantly lower. All patients with malignant lesions were identified by CTex, VIBEin, and VIBEex, while 3 patients were false-negative in Dixon. Conclusion Fast T1-weighted VIBE sequences allow for identification of patients with malignant pulmonary lesions. The Dixon sequence is not recommended for lung nodule evaluation in oncologic MR

  6. Binocular and multi-view parallax images acquisition for three dimensional stereoscopic displays

    NASA Astrophysics Data System (ADS)

    Ge, Hongsheng; Sang, Xinzhu; Zhao, Tianqi; Yuan, Jinhui; Leng, Junmin; Zhang, Ying; Yan, Binbin

    2012-11-01

    It is important to acquire the proper parallax images for the stereoscopic display system. By setting the proper distance between the cameras and the location of the convergent point in this capturing configuration, the displayed 3D scene with the appropriate stereo depth and the expected effect in front of and behind the display screen can be obtained directly. The quantitative relationship between the parallax and the parameters of the capturing configuration with two cameras is presented. The capturing system with multiple cameras for acquiring equal parallaxes between the adjacent captured images for the autostereoscopic display system is also discussed. The proposed methods are demonstrated by the experimental results. The captured images with the calculated parameters for the 3D display system shows the expected results, which can provide the viewers the better immersion and visual comfort without any extra processing.

  7. A new system for digital image acquisition, storage and presentation in an accident and emergency department

    PubMed Central

    Clegg, G; Roebuck, S; Steedman, D

    2001-01-01

    Objectives—To develop a computer based storage system for clinical images—radiographs, photographs, ECGs, text—for use in teaching, training, reference and research within an accident and emergency (A&E) department. Exploration of methods to access and utilise the data stored in the archive. Methods—Implementation of a digital image archive using flatbed scanner and digital camera as capture devices. A sophisticated coding system based on ICD 10. Storage via an "intelligent" custom interface. Results—A practical solution to the problems of clinical image storage for teaching purposes. Conclusions—We have successfully developed a digital image capture and storage system, which provides an excellent teaching facility for a busy A&E department. We have revolutionised the practice of the "hand-over meeting". PMID:11435357

  8. High-speed multiframe dynamic transmission electron microscope image acquisition system with arbitrary timing

    DOEpatents

    Reed, Bryan W.; DeHope, William J.; Huete, Glenn; LaGrange, Thomas B.; Shuttlesworth, Richard M.

    2015-10-20

    An electron microscope is disclosed which has a laser-driven photocathode and an arbitrary waveform generator (AWG) laser system ("laser"). The laser produces a train of temporally-shaped laser pulses of a predefined pulse duration and waveform, and directs the laser pulses to the laser-driven photocathode to produce a train of electron pulses. An image sensor is used along with a deflector subsystem. The deflector subsystem is arranged downstream of the target but upstream of the image sensor, and has two pairs of plates arranged perpendicular to one another. A control system controls the laser and a plurality of switching components synchronized with the laser, to independently control excitation of each one of the deflector plates. This allows each electron pulse to be directed to a different portion of the image sensor, as well as to be provided with an independently set duration and independently set inter-pulse spacings.

  9. High-speed multiframe dynamic transmission electron microscope image acquisition system with arbitrary timing

    DOEpatents

    Reed, Bryan W.; Dehope, William J; Huete, Glenn; LaGrange, Thomas B.; Shuttlesworth, Richard M

    2016-06-21

    An electron microscope is disclosed which has a laser-driven photocathode and an arbitrary waveform generator (AWG) laser system ("laser"). The laser produces a train of temporally-shaped laser pulses of a predefined pulse duration and waveform, and directs the laser pulses to the laser-driven photocathode to produce a train of electron pulses. An image sensor is used along with a deflector subsystem. The deflector subsystem is arranged downstream of the target but upstream of the image sensor, and has two pairs of plates arranged perpendicular to one another. A control system controls the laser and a plurality of switching components synchronized with the laser, to independently control excitation of each one of the deflector plates. This allows each electron pulse to be directed to a different portion of the image sensor, as well as to be provided with an independently set duration and independently set inter-pulse spacings.

  10. High-speed multi-frame dynamic transmission electron microscope image acquisition system with arbitrary timing

    SciTech Connect

    Reed, Bryan W.; DeHope, William J.; Huete, Glenn; LaGrange, Thomas B.; Shuttlesworth, Richard M.

    2016-02-23

    An electron microscope is disclosed which has a laser-driven photocathode and an arbitrary waveform generator (AWG) laser system ("laser"). The laser produces a train of temporally-shaped laser pulses each being of a programmable pulse duration, and directs the laser pulses to the laser-driven photocathode to produce a train of electron pulses. An image sensor is used along with a deflector subsystem. The deflector subsystem is arranged downstream of the target but upstream of the image sensor, and has a plurality of plates. A control system having a digital sequencer controls the laser and a plurality of switching components, synchronized with the laser, to independently control excitation of each one of the deflector plates. This allows each electron pulse to be directed to a different portion of the image sensor, as well as to enable programmable pulse durations and programmable inter-pulse spacings.

  11. Wireless photoplethysmographic device for heart rate variability signal acquisition and analysis.

    PubMed

    Reyes, Ivan; Nazeran, Homer; Franco, Mario; Haltiwanger, Emily

    2012-01-01

    The photoplethysmographic (PPG) signal has the potential to aid in the acquisition and analysis of heart rate variability (HRV) signal: a non-invasive quantitative marker of the autonomic nervous system that could be used to assess cardiac health and other physiologic conditions. A low-power wireless PPG device was custom-developed to monitor, acquire and analyze the arterial pulse in the finger. The system consisted of an optical sensor to detect arterial pulse as variations in reflected light intensity, signal conditioning circuitry to process the reflected light signal, a microcontroller to control PPG signal acquisition, digitization and wireless transmission, a receiver to collect the transmitted digital data and convert them back to their analog representations. A personal computer was used to further process the captured PPG signals and display them. A MATLAB program was then developed to capture the PPG data, detect the RR peaks, perform spectral analysis of the PPG data, and extract the HRV signal. A user-friendly graphical user interface (GUI) was developed in LabView to display the PPG data and their spectra. The performance of each module (sensing unit, signal conditioning, wireless transmission/reception units, and graphical user interface) was assessed individually and the device was then tested as a whole. Consequently, PPG data were obtained from five healthy individuals to test the utility of the wireless system. The device was able to reliably acquire the PPG signals from the volunteers. To validate the accuracy of the MATLAB codes, RR peak information from each subject was fed into Kubios software as a text file. Kubios was able to generate a report sheet with the time domain and frequency domain parameters of the acquired data. These features were then compared against those calculated by MATLAB. The preliminary results demonstrate that the prototype wireless device could be used to perform HRV signal acquisition and analysis.

  12. The Probabilistic Analysis of Language Acquisition: Theoretical, Computational, and Experimental Analysis

    ERIC Educational Resources Information Center

    Hsu, Anne S.; Chater, Nick; Vitanyi, Paul M. B.

    2011-01-01

    There is much debate over the degree to which language learning is governed by innate language-specific biases, or acquired through cognition-general principles. Here we examine the probabilistic language acquisition hypothesis on three levels: We outline a novel theoretical result showing that it is possible to learn the exact "generative model"…

  13. Image acquisition, geometric correction and display of images from a 2×2 x-ray detector array based on Electron Multiplying Charge Coupled Device (EMCCD) technology.

    PubMed

    Vasan, S N Swetadri; Sharma, P; Ionita, Ciprian N; Titus, A H; Cartwright, A N; Bednarek, D R; Rudin, S

    2013-03-06

    A high resolution (up to 11.2 lp/mm) x-ray detector with larger field of view (8.5 cm × 8.5 cm) has been developed. The detector is a 2 × 2 array of individual imaging modules based on EMCCD technology. Each module outputs a frame of size 1088 × 1037 pixels, each 12 bits. The frames from the 4 modules are acquired into the processing computer using one of two techniques. The first uses 2 CameraLink communication channels with each carrying information from two modules, the second uses a application specific custom integrated circuits, the Multiple Module Multiplexer Integrated Circuit (MMMIC), 3 of which are used to multiplex the data from 4 modules into one CameraLink channel. Once the data is acquired using either of the above mentioned techniques, it is decoded in the graphics processing unit (GPU) to form one single frame of size 2176 × 2074 pixels each 16 bits. Each imaging module uses a fiber optic taper coupled to the EMCCD sensor. To correct for mechanical misalignment between the sensors and the fiber optic tapers and produce a single seamless image, the images in each module may be rotated and translated slightly in the x-y plane with respect to each other. To evaluate the detector acquisition and correction techniques, an aneurysm model was placed over an anthropomorphic head phantom and a coil was guided into the aneurysm under fluoroscopic guidance using the detector array. Image sequences before and after correction are presented which show near-seamless boundary matching and are well suited for fluoroscopic imaging.

  14. Acquisition of Diagnostic Screen and Synchrotron Radiation Images Using IEEE1394 Digital Cameras

    NASA Astrophysics Data System (ADS)

    Rehm, G.

    2004-11-01

    In the LINAC, booster synchrotron and transfer lines of DIAMOND a number of screens (YAG:Ce and OTR) as well as synchrotron radiation ports will be used to acquire information about the transverse beam distribution. Digital IEEE1394 cameras have been selected for their range of sensor sizes and resolutions available, their easy triggering to single events, and their noise-free transmission of the images into the control system. Their suitability for use under influence of high-energy radiation has been verified. Images from preliminary tests at the SRS Daresbury are presented.

  15. Acquisition of quantitative physiological data and computerized image reconstruction using a single scan TV system

    NASA Technical Reports Server (NTRS)

    Baily, N. A.

    1975-01-01

    Single scan operation of television X-ray fluoroscopic systems allow both analog and digital reconstruction of tomographic sections from single plan images. This type of system combined with a minimum of statistical processing showed excellent capabilities for delineating small changes in differential X-ray attenuation. Patient dose reduction is significant when compared to normal operation or film recording. Flat screen, low light level systems were both rugged and light in weight, making them applicable for a variety of special purposes. Three dimensional information was available from the tomographic methods and the recorded data was sufficient when used with appropriate computer display devices to give representative 3D images.

  16. SLControl: PC-based data acquisition and analysis for muscle mechanics.

    PubMed

    Campbell, Kenneth S; Moss, Richard L

    2003-12-01

    SLControl is a computerized data acquisition and analysis system that was developed in our laboratory to help perform mechanical experiments using striated muscle preparations. It consists of a computer program (Windows 2000 or later) and a commercially available data acquisition board (16-bit resolution, DAP5216a, Microstar Laboratories, Bellevue, WA). Signals from the user's existing equipment representing force, fiber length (FL), and (if desired) sarcomere length (SL) are connected to the system through standard Bayonet Neill Concelman cables and saved to data files for later analysis. Output signals from the board control FL and trigger additional equipment, e.g., flash lamps. Windows dialogs drive several different experimental protocols, including slack tests and rate of tension recovery measurements. Precise measurements of muscle stiffness and force velocity/power characteristics can also be accomplished using SL and tension control, respectively. In these situations, the FL command signal is updated in real time (at rates > or =2.5 kHz) in response to changes in the measured SL or force signals. Data files can be exported as raw text or analyzed within SLControl with the use of built-in tools for cursor analysis, digital filtering, curve fitting, etc. The software is available for free download at http://www.slcontrol.com.

  17. 48 CFR 52.223-13 - Acquisition of EPEAT®-Registered Imaging Equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) Definitions. As used in this clause— Imaging equipment means the following products: (1) Copier—A commercially... connection. This definition is intended to cover products that are marketed as copiers or upgradeable digital... connection. This definition is intended to cover products that are marketed as digital duplicators....

  18. Improving in situ data acquisition using training images and a Bayesian mixture model

    NASA Astrophysics Data System (ADS)

    Abdollahifard, Mohammad Javad; Mariethoz, Gregoire; Pourfard, Mohammadreza

    2016-06-01

    Estimating the spatial distribution of physical processes using a minimum number of samples is of vital importance in earth science applications where sampling is costly. In recent years, training image-based methods have received a lot of attention for interpolation and simulation. However, training images have never been employed to optimize spatial sampling process. In this paper, a sequential compressive sampling method is presented which decides the location of new samples based on a training image. First, a Bayesian mixture model is developed based on the training patterns. Then, using this model, unknown values are estimated based on a limited number of random samples. Since the model is probabilistic, it allows estimating local uncertainty conditionally to the available samples. Based on this, new samples are sequentially extracted from the locations with maximum uncertainty. Experiments show that compared to a random sampling strategy, the proposed supervised sampling method significantly reduces the number of samples needed to achieve the same level of accuracy, even when the training image is not optimally chosen. The method has the potential to reduce the number of observations necessary for the characterization of environmental processes.

  19. Teaching the Dance Class: Strategies to Enhance Skill Acquisition, Mastery and Positive Self-Image

    ERIC Educational Resources Information Center

    Mainwaring, Lynda M.; Krasnow, Donna H.

    2010-01-01

    Effective teaching of dance skills is informed by a variety of theoretical frameworks and individual teaching and learning styles. The purpose of this paper is to present practical teaching strategies that enhance the mastery of skills and promote self-esteem, self-efficacy, and positive self-image. The predominant thinking and primary research…

  20. Radiation acquisition and RBF neural network analysis on BOF end-point control

    NASA Astrophysics Data System (ADS)

    Zhao, Qi; Wen, Hong-yuan; Zhou, Mu-chun; Chen, Yan-ru

    2008-12-01

    There are some problems in Basic Oxygen Furnace (BOF) steelmaking end-point control technology at present. A new BOF end-point control model was designed, which was based on the character of carbon oxygen reaction in Basic Oxygen Furnace steelmaking process. The image capture and transformation system was established by Video for Windows (VFW) library function, which is a video software development package promoted by Microsoft Corporation. In this paper, the Radial Basic Function (RBF) neural network model was established by using the real-time acquisition information. The input parameters can acquire easily online and the output parameter is the end-point time, which can compare with the actual value conveniently. The experience results show that the predication result is ideal and the experiment results show the model can work well in the steelmaking adverse environment.

  1. MRI Image Processing Based on Fractal Analysis

    PubMed

    Marusina, Mariya Y; Mochalina, Alexandra P; Frolova, Ekaterina P; Satikov, Valentin I; Barchuk, Anton A; Kuznetcov, Vladimir I; Gaidukov, Vadim S; Tarakanov, Segrey A

    2017-01-01

    Background: Cancer is one of the most common causes of human mortality, with about 14 million new cases and 8.2 million deaths reported in in 2012. Early diagnosis of cancer through screening allows interventions to reduce mortality. Fractal analysis of medical images may be useful for this purpose. Materials and Methods: In this study, we examined magnetic resonance (MR) images of healthy livers and livers containing metastases from colorectal cancer. The fractal dimension and the Hurst exponent were chosen as diagnostic features for tomographic imaging using Image J software package for image processings FracLac for applied for fractal analysis with a 120x150 pixel area. Calculations of the fractal dimensions of pathological and healthy tissue samples were performed using the box-counting method. Results: In pathological cases (foci formation), the Hurst exponent was less than 0.5 (the region of unstable statistical characteristics). For healthy tissue, the Hurst index is greater than 0.5 (the zone of stable characteristics). Conclusions: The study indicated the possibility of employing fractal rapid analysis for the detection of focal lesions of the liver. The Hurst exponent can be used as an important diagnostic characteristic for analysis of medical images.

  2. Retinal imaging analysis based on vessel detection.

    PubMed

    Jamal, Arshad; Hazim Alkawaz, Mohammed; Rehman, Amjad; Saba, Tanzila

    2017-03-13

    With an increase in the advancement of digital imaging and computing power, computationally intelligent technologies are in high demand to be used in ophthalmology cure and treatment. In current research, Retina Image Analysis (RIA) is developed for optometrist at Eye Care Center in Management and Science University. This research aims to analyze the retina through vessel detection. The RIA assists in the analysis of the retinal images and specialists are served with various options like saving, processing and analyzing retinal images through its advanced interface layout. Additionally, RIA assists in the selection process of vessel segment; processing these vessels by calculating its diameter, standard deviation, length, and displaying detected vessel on the retina. The Agile Unified Process is adopted as the methodology in developing this research. To conclude, Retina Image Analysis might help the optometrist to get better understanding in analyzing the patient's retina. Finally, the Retina Image Analysis procedure is developed using MATLAB (R2011b). Promising results are attained that are comparable in the state of art.

  3. Lexical Link Analysis Application: Improving Web Service to Acquisition Visibility Portal Phase III

    DTIC Science & Technology

    2015-04-30

    ååì~ä=^Åèìáëáíáçå= oÉëÉ~êÅÜ=póãéçëáìã= qÜìêëÇ~ó=pÉëëáçåë= sçäìãÉ=ff= = Lexical Link Analysis Application: Improving Web Service to Acquisition...2015 2. REPORT TYPE 3. DATES COVERED 00-00-2015 to 00-00-2015 4. TITLE AND SUBTITLE Lexical Link Analysis Application: Improving Web Service...www.acquisitionresearch.net). ^Åèìáëáíáçå=oÉëÉ~êÅÜ=mêçÖê~ãW= `êÉ~íáåÖ=póåÉêÖó=Ñçê=fåÑçêãÉÇ=`Ü~åÖÉ= - 345 - Lexical Link Analysis Application: Improving Web Service

  4. Simultaneous analysis and quality assurance for diffusion tensor imaging.

    PubMed

    Lauzon, Carolyn B; Asman, Andrew J; Esparza, Michael L; Burns, Scott S; Fan, Qiuyun; Gao, Yurui; Anderson, Adam W; Davis, Nicole; Cutting, Laurie E; Landman, Bennett A

    2013-01-01

    Diffusion tensor imaging (DTI) enables non-invasive, cyto-architectural mapping of in vivo tissue microarchitecture through voxel-wise mathematical modeling of multiple magnetic resonance imaging (MRI) acquisitions, each differently sensitized to water diffusion. DTI computations are fundamentally estimation processes and are sensitive to noise and artifacts. Despite widespread adoption in the neuroimaging community, maintaining consistent DTI data quality remains challenging given the propensity for patient motion, artifacts associated with fast imaging techniques, and the possibility of hardware changes/failures. Furthermore, the quantity of data acquired per voxel, the non-linear estimation process, and numerous potential use cases complicate traditional visual data inspection approaches. Currently, quality inspection of DTI data has relied on visual inspection and individual processing in DTI analysis software programs (e.g. DTIPrep, DTI-studio). However, recent advances in applied statistical methods have yielded several different metrics to assess noise level, artifact propensity, quality of tensor fit, variance of estimated measures, and bias in estimated measures. To date, these metrics have been largely studied in isolation. Herein, we select complementary metrics for integration into an automatic DTI analysis and quality assurance pipeline. The pipeline completes in 24 hours, stores statistical outputs, and produces a graphical summary quality analysis (QA) report. We assess the utility of this streamlined approach for empirical quality assessment on 608 DTI datasets from pediatric neuroimaging studies. The efficiency and accuracy of quality analysis using the proposed pipeline is compared with quality analysis based on visual inspection. The unified pipeline is found to save a statistically significant amount of time (over 70%) while improving the consistency of QA between a DTI expert and a pool of research associates. Projection of QA metrics to a low

  5. Simultaneous Analysis and Quality Assurance for Diffusion Tensor Imaging

    PubMed Central

    Lauzon, Carolyn B.; Asman, Andrew J.; Esparza, Michael L.; Burns, Scott S.; Fan, Qiuyun; Gao, Yurui; Anderson, Adam W.; Davis, Nicole; Cutting, Laurie E.; Landman, Bennett A.

    2013-01-01

    Diffusion tensor imaging (DTI) enables non-invasive, cyto-architectural mapping of in vivo tissue microarchitecture through voxel-wise mathematical modeling of multiple magnetic resonance imaging (MRI) acquisitions, each differently sensitized to water diffusion. DTI computations are fundamentally estimation processes and are sensitive to noise and artifacts. Despite widespread adoption in the neuroimaging community, maintaining consistent DTI data quality remains challenging given the propensity for patient motion, artifacts associated with fast imaging techniques, and the possibility of hardware changes/failures. Furthermore, the quantity of data acquired per voxel, the non-linear estimation process, and numerous potential use cases complicate traditional visual data inspection approaches. Currently, quality inspection of DTI data has relied on visual inspection and individual processing in DTI analysis software programs (e.g. DTIPrep, DTI-studio). However, recent advances in applied statistical methods have yielded several different metrics to assess noise level, artifact propensity, quality of tensor fit, variance of estimated measures, and bias in estimated measures. To date, these metrics have been largely studied in isolation. Herein, we select complementary metrics for integration into an automatic DTI analysis and quality assurance pipeline. The pipeline completes in 24 hours, stores statistical outputs, and produces a graphical summary quality analysis (QA) report. We assess the utility of this streamlined approach for empirical quality assessment on 608 DTI datasets from pediatric neuroimaging studies. The efficiency and accuracy of quality analysis using the proposed pipeline is compared with quality analysis based on visual inspection. The unified pipeline is found to save a statistically significant amount of time (over 70%) while improving the consistency of QA between a DTI expert and a pool of research associates. Projection of QA metrics to a low

  6. Proteomic analysis of formalin-fixed paraffin embedded tissue by MALDI imaging mass spectrometry

    PubMed Central

    Casadonte, Rita; Caprioli, Richard M

    2012-01-01

    Archived formalin-fixed paraffin-embedded (FFPE) tissue collections represent a valuable informational resource for proteomic studies. Multiple FFPE core biopsies can be assembled in a single block to form tissue microarrays (TMAs). We describe a protocol for analyzing protein in FFPE -TMAs using matrix-assisted laser desorption/ionization (MAL DI) imaging mass spectrometry (IMS). The workflow incorporates an antigen retrieval step following deparaffinization, in situ trypsin digestion, matrix application and then mass spectrometry signal acquisition. The direct analysis of FFPE -TMA tissue using IMS allows direct analysis of multiple tissue samples in a single experiment without extraction and purification of proteins. The advantages of high speed and throughput, easy sample handling and excellent reproducibility make this technology a favorable approach for the proteomic analysis of clinical research cohorts with large sample numbers. For example, TMA analysis of 300 FFPE cores would typically require 6 h of total time through data acquisition, not including data analysis. PMID:22011652

  7. Automated approaches for analysis of multimodal MRI acquisitions in a study of cognitive aging.

    PubMed

    Hodneland, Erlend; Ystad, Martin; Haasz, Judit; Munthe-Kaas, Antonella; Lundervold, Arvid

    2012-06-01

    In this work we describe an integrated and automated workflow for a comprehensive and robust analysis of multimodal MR images from a cohort of more than hundred subjects. Image examinations are done three years apart and consist of 3D high-resolution anatomical images, low resolution tensor-valued DTI recordings and 4D resting state fMRI time series. The integrated analysis of the data requires robust tools for segmentation, registration and fiber tracking, which we combine in an automated manner. Our automated workflow is strongly desired due to the large number of subjects. Especially, we introduce the use of histogram segmentation to processed fMRI data to obtain functionally important seed and target regions for fiber tracking between them. This enables analysis of individually important resting state networks. We also discuss various approaches for the assessment of white matter integrity parameters along tracts, and in particular we introduce the use of functional data analysis (FDA) for this task.

  8. Analysis of Performance-Based Service Contracting and Its Applicability to Turkey’s Defense Acquisition Activities

    DTIC Science & Technology

    2010-12-01

    ABSTRACT The purpose of this MBA professional report is to explore and analyze Performance Based Service Contracting (PBSC) and provide a clear...ANALYSIS OF PERFORMANCE BASED SERVICE CONTRACTING AND ITS APPLICABILITY TO TURKEY’S DEFENSE ACQUISITION ACTIVITIES ABSTRACT The purpose of this MBA...Policy (1998): Performance-Based Service Contracting (PBSC) emphasizes that all aspects of an acquisition be structured around the purpose of the

  9. Single particle raster image analysis of diffusion.

    PubMed

    Longfils, M; Schuster, E; Lorén, N; Särkkä, A; Rudemo, M

    2017-04-01

    As a complement to the standard RICS method of analysing Raster Image Correlation Spectroscopy images with estimation of the image correlation function, we introduce the method SPRIA, Single Particle Raster Image Analysis. Here, we start by identifying individual particles and estimate the diffusion coefficient for each particle by a maximum likelihood method. Averaging over the particles gives a diffusion coefficient estimate for the whole image. In examples both with simulated and experimental data, we show that the new method gives accurate estimates. It also gives directly standard error estimates. The method should be possible to extend to study heterogeneous materials and systems of particles with varying diffusion coefficient, as demonstrated in a simple simulation example. A requirement for applying the SPRIA method is that the particle concentration is low enough so that we can identify the individual particles. We also describe a bootstrap method for estimating the standard error of standard RICS.

  10. Acquisition of quantitative physiological data and computerized image reconstruction using a single scan TV system

    NASA Technical Reports Server (NTRS)

    Baily, N. A.

    1976-01-01

    A single-scan radiography system has been interfaced to a minicomputer, and the combined system has been used with a variety of fluoroscopic systems and image intensifiers available in clinical facilities. The system's response range is analyzed, and several applications are described. These include determination of the gray scale for typical X-ray-fluoroscopic-television chains, measurement of gallstone volume in patients, localization of markers or other small anatomical features, determinations of organ areas and volumes, computer reconstruction of tomographic sections of organs in motion, and computer reconstruction of transverse axial body sections from fluoroscopic images. It is concluded that this type of system combined with a minimum of statistical processing shows excellent capabilities for delineating small changes in differential X-ray attenuation.

  11. A Preanalytic Validation Study of Automated Bone Scan Index: Effect on Accuracy and Reproducibility Due to the Procedural Variabilities in Bone Scan Image Acquisition.

    PubMed

    Anand, Aseem; Morris, Michael J; Kaboteh, Reza; Reza, Mariana; Trägårdh, Elin; Matsunaga, Naofumi; Edenbrandt, Lars; Bjartell, Anders; Larson, Steven M; Minarik, David

    2016-12-01

    The effect of the procedural variability in image acquisition on the quantitative assessment of bone scan is unknown. Here, we have developed and performed preanalytical studies to assess the impact of the variability in scanning speed and in vendor-specific γ-camera on reproducibility and accuracy of the automated bone scan index (BSI).

  12. Functional data analysis in brain imaging studies.

    PubMed

    Tian, Tian Siva

    2010-01-01

    Functional data analysis (FDA) considers the continuity of the curves or functions, and is a topic of increasing interest in the statistics community. FDA is commonly applied to time-series and spatial-series studies. The development of functional brain imaging techniques in recent years made it possible to study the relationship between brain and mind over time. Consequently, an enormous amount of functional data is collected and needs to be analyzed. Functional techniques designed for these data are in strong demand. This paper discusses three statistically challenging problems utilizing FDA techniques in functional brain imaging analysis. These problems are dimension reduction (or feature extraction), spatial classification in functional magnetic resonance imaging studies, and the inverse problem in magneto-encephalography studies. The application of FDA to these issues is relatively new but has been shown to be considerably effective. Future efforts can further explore the potential of FDA in functional brain imaging studies.

  13. Particle Pollution Estimation Based on Image Analysis

    PubMed Central

    Liu, Chenbin; Tsow, Francis; Zou, Yi; Tao, Nongjian

    2016-01-01

    Exposure to fine particles can cause various diseases, and an easily accessible method to monitor the particles can help raise public awareness and reduce harmful exposures. Here we report a method to estimate PM air pollution based on analysis of a large number of outdoor images available for Beijing, Shanghai (China) and Phoenix (US). Six image features were extracted from the images, which were used, together with other relevant data, such as the position of the sun, date, time, geographic information and weather conditions, to predict PM2.5 index. The results demonstrate that the image analysis method provides good prediction of PM2.5 indexes, and different features have different significance levels in the prediction. PMID:26828757

  14. Particle Pollution Estimation Based on Image Analysis.

    PubMed

    Liu, Chenbin; Tsow, Francis; Zou, Yi; Tao, Nongjian

    2016-01-01

    Exposure to fine particles can cause various diseases, and an easily accessible method to monitor the particles can help raise public awareness and reduce harmful exposures. Here we report a method to estimate PM air pollution based on analysis of a large number of outdoor images available for Beijing, Shanghai (China) and Phoenix (US). Six image features were extracted from the images, which were used, together with other relevant data, such as the position of the sun, date, time, geographic information and weather conditions, to predict PM2.5 index. The results demonstrate that the image analysis method provides good prediction of PM2.5 indexes, and different features have different significance levels in the prediction.

  15. Quantitative analysis of qualitative images

    NASA Astrophysics Data System (ADS)

    Hockney, David; Falco, Charles M.

    2005-03-01

    We show optical evidence that demonstrates artists as early as Jan van Eyck and Robert Campin (c1425) used optical projections as aids for producing their paintings. We also have found optical evidence within works by later artists, including Bermejo (c1475), Lotto (c1525), Caravaggio (c1600), de la Tour (c1650), Chardin (c1750) and Ingres (c1825), demonstrating a continuum in the use of optical projections by artists, along with an evolution in the sophistication of that use. However, even for paintings where we have been able to extract unambiguous, quantitative evidence of the direct use of optical projections for producing certain of the features, this does not mean that paintings are effectively photographs. Because the hand and mind of the artist are intimately involved in the creation process, understanding these complex images requires more than can be obtained from only applying the equations of geometrical optics.

  16. On Two-Dimensional ARMA Models for Image Analysis.

    DTIC Science & Technology

    1980-03-24

    2-D ARMA models for image analysis . Particular emphasis is placed on restoration of noisy images using 2-D ARMA models. Computer results are...is concluded that the models are very effective linear models for image analysis . (Author)

  17. Probabilistic Round Trip Contamination Analysis of a Mars Sample Acquisition and Handling Process Using Markovian Decompositions

    NASA Technical Reports Server (NTRS)

    Hudson, Nicolas; Lin, Ying; Barengoltz, Jack

    2010-01-01

    A method for evaluating the probability of a Viable Earth Microorganism (VEM) contaminating a sample during the sample acquisition and handling (SAH) process of a potential future Mars Sample Return mission is developed. A scenario where multiple core samples would be acquired using a rotary percussive coring tool, deployed from an arm on a MER class rover is analyzed. The analysis is conducted in a structured way by decomposing sample acquisition and handling process into a series of discrete time steps, and breaking the physical system into a set of relevant components. At each discrete time step, two key functions are defined: The probability of a VEM being released from each component, and the transport matrix, which represents the probability of VEM transport from one component to another. By defining the expected the number of VEMs on each component at the start of the sampling process, these decompositions allow the expected number of VEMs on each component at each sampling step to be represented as a Markov chain. This formalism provides a rigorous mathematical framework in which to analyze the probability of a VEM entering the sample chain, as well as making the analysis tractable by breaking the process down into small analyzable steps.

  18. On-line acquisition, analysis and presentation of neurophysiological data based on a personal microcomputer system.

    PubMed

    Stromquist, B R; Pavlides, C; Zelano, J A

    1990-12-01

    A microcomputer based system is described for the acquisition, averaging, displaying, analysis and storage of electrophysiological (EPSP and post-stimulus histogram) data. The system consists of commercially available hardware (IBM-PC AT compatible, 80286 or 80386 based microcomputer, Burr-Brown analog-to-digital (A/D) converter), a custom built interface module, and a combination of commercially available and custom built software packages. The software operates within a Microsoft Windows environment and is comprised of custom built data acquisition and review modules which are linked to Microsoft's Excel program. The system is capable of four channel A/D conversion of EPSP's at a sampling frequency of up to 10 KHz (50 KHz single channel), the averaging of data including the addition and subtraction of various channels, the graphical display of data, the extraction of various data parameters, and the transfer of data to an Excel spreadsheet. The spreadsheet allows for the development of mathematical formulas for statistical analysis of data and presentation of the results in graphical form. Finally, data can easily be output to a laser printer or plotter. A sample experiment, illustrating system operation, is presented.

  19. Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans.

    PubMed

    Dahlin, Paul; Srivastava, Vaibhav; Ekengren, Sophia; McKee, Lauren S; Bulone, Vincent

    2017-01-01

    The oomycete class includes pathogens of animals and plants which are responsible for some of the most significant global losses in agriculture and aquaculture. There is a need to replace traditional chemical means of controlling oomycete growth with more targeted approaches, and the inhibition of sterol synthesis is one promising area. To better direct these efforts, we have studied sterol acquisition in two model organisms: the sterol-autotrophic Saprolegnia parasitica, and the sterol-heterotrophic Phytophthora infestans. We first present a comprehensive reconstruction of a likely sterol synthesis pathway for S. parasitica, causative agent of the disease saprolegniasis in fish. This pathway shows multiple potential routes of sterol synthesis, and draws on several avenues of new evidence: bioinformatic mining for genes with sterol-related functions, expression analysis of these genes, and analysis of the sterol profiles in mycelium grown in different media. Additionally, we explore the extent to which P. infestans, which causes the late blight in potato, can modify exogenously provided sterols. We consider whether the two very different approaches to sterol acquisition taken by these pathogens represent any specific survival advantages or potential drug targets.

  20. Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans

    PubMed Central

    Dahlin, Paul; Srivastava, Vaibhav; Ekengren, Sophia; McKee, Lauren S.; Bulone, Vincent

    2017-01-01

    The oomycete class includes pathogens of animals and plants which are responsible for some of the most significant global losses in agriculture and aquaculture. There is a need to replace traditional chemical means of controlling oomycete growth with more targeted approaches, and the inhibition of sterol synthesis is one promising area. To better direct these efforts, we have studied sterol acquisition in two model organisms: the sterol-autotrophic Saprolegnia parasitica, and the sterol-heterotrophic Phytophthora infestans. We first present a comprehensive reconstruction of a likely sterol synthesis pathway for S. parasitica, causative agent of the disease saprolegniasis in fish. This pathway shows multiple potential routes of sterol synthesis, and draws on several avenues of new evidence: bioinformatic mining for genes with sterol-related functions, expression analysis of these genes, and analysis of the sterol profiles in mycelium grown in different media. Additionally, we explore the extent to which P. infestans, which causes the late blight in potato, can modify exogenously provided sterols. We consider whether the two very different approaches to sterol acquisition taken by these pathogens represent any specific survival advantages or potential drug targets. PMID:28152045

  1. AMA Statistical Information Based Analysis of a Compressive Imaging System

    NASA Astrophysics Data System (ADS)

    Hope, D.; Prasad, S.

    Recent advances in optics and instrumentation have dramatically increased the amount of data, both spatial and spectral, that can be obtained about a target scene. The volume of the acquired data can and, in fact, often does far exceed the amount of intrinsic information present in the scene. In such cases, the large volume of data alone can impede the analysis and extraction of relevant information about the scene. One approach to overcoming this impedance mismatch between the volume of data and intrinsic information in the scene the data are supposed to convey is compressive sensing. Compressive sensing exploits the fact that most signals of interest, such as image scenes, possess natural correlations in their physical structure. These correlations, which can occur spatially as well as spectrally, can suggest a more natural sparse basis for compressing and representing the scene than standard pixels or voxels. A compressive sensing system attempts to acquire and encode the scene in this sparse basis, while preserving all relevant information in the scene. One criterion for assessing the content, acquisition, and processing of information in the image scene is Shannon information. This metric describes fundamental limits on encoding and reliably transmitting information about a source, such as an image scene. In this framework, successful encoding of the image requires an optimal choice of a sparse basis, while losses of information during transmission occur due to a finite system response and measurement noise. An information source can be represented by a certain class of image scenes, .e.g. those that have a common morphology. The ability to associate the recorded image with the correct member of the class that produced the image depends on the amount of Shannon information in the acquired data. In this manner, one can analyze the performance of a compressive imaging system for a specific class or ensemble of image scenes. We present such an information

  2. VAICo: visual analysis for image comparison.

    PubMed

    Schmidt, Johanna; Gröller, M Eduard; Bruckner, Stefan

    2013-12-01

    Scientists, engineers, and analysts are confronted with ever larger and more complex sets of data, whose analysis poses special challenges. In many situations it is necessary to compare two or more datasets. Hence there is a need for comparative visualization tools to help analyze differences or similarities among datasets. In this paper an approach for comparative visualization for sets of images is presented. Well-established techniques for comparing images frequently place them side-by-side. A major drawback of such approaches is that they do not scale well. Other image comparison methods encode differences in images by abstract parameters like color. In this case information about the underlying image data gets lost. This paper introduces a new method for visualizing differences and similarities in large sets of images which preserves contextual information, but also allows the detailed analysis of subtle variations. Our approach identifies local changes and applies cluster analysis techniques to embed them in a hierarchy. The results of this process are then presented in an interactive web application which allows users to rapidly explore the space of differences and drill-down on particular features. We demonstrate the flexibility of our approach by applying it to multiple distinct domains.

  3. From Image Analysis to Computer Vision: Motives, Methods, and Milestones.

    DTIC Science & Technology

    1998-07-01

    images. Initially, work on digital image analysis dealt with specific classes of images such as text, photomicrographs, nuclear particle tracks, and aerial...photographs; but by the 1960’s, general algorithms and paradigms for image analysis began to be formulated. When the artificial intelligence...scene, but eventually from image sequences obtained by a moving camera; at this stage, image analysis had become scene analysis or computer vision

  4. Skeletal muscle diffusion tensor-MRI fiber tracking: rationale, data acquisition and analysis methods, applications and future directions.

    PubMed

    Damon, Bruce M; Froeling, Martijn; Buck, Amanda K W; Oudeman, Jos; Ding, Zhaohua; Nederveen, Aart J; Bush, Emily C; Strijkers, Gustav J

    2017-03-01

    The mechanical functions of muscles involve the generation of force and the actuation of movement by shortening or lengthening under load. These functions are influenced, in part, by the internal arrangement of muscle fibers with respect to the muscle's mechanical line of action. This property is known as muscle architecture. In this review, we describe the use of diffusion tensor (DT)-MRI muscle fiber tracking for the study of muscle architecture. In the first section, the importance of skeletal muscle architecture to function is discussed. In addition, traditional and complementary methods for the assessment of muscle architecture (brightness-mode ultrasound imaging and cadaver analysis) are presented. Next, DT-MRI is introduced and the structural basis for the reduced and anisotropic diffusion of water in muscle is discussed. The third section discusses issues related to the acquisition of skeletal muscle DT-MRI data and presents recommendations for optimal strategies. The fourth section discusses methods for the pre-processing of DT-MRI data, the available approaches for the calculation of the diffusion tensor and the seeding and propagating of fiber tracts, and the analysis of the tracking results to measure structural properties pertinent to muscle biomechanics. Lastly, examples are presented of how DT-MRI fiber tracking has been used to provide new insights into how muscles function, and important future research directions are highlighted. Copyright © 2016 John Wiley & Sons, Ltd.

  5. Selecting an image analysis minicomputer system

    NASA Technical Reports Server (NTRS)

    Danielson, R.

    1981-01-01

    Factors to be weighed when selecting a minicomputer system as the basis for an image analysis computer facility vary depending on whether the user organization procures a new computer or selects an existing facility to serve as an image analysis host. Some conditions not directly related to hardware or software should be considered such as the flexibility of the computer center staff, their encouragement of innovation, and the availability of the host processor to a broad spectrum of potential user organizations. Particular attention must be given to: image analysis software capability; the facilities of a potential host installation; the central processing unit; the operating system and languages; main memory; disk storage; tape drives; hardcopy output; and other peripherals. The operational environment, accessibility; resource limitations; and operational supports are important. Charges made for program execution and data storage must also be examined.

  6. Automated video-microscopic imaging and data acquisition system for colloid deposition measurements

    DOEpatents

    Abdel-Fattah, Amr I.; Reimus, Paul W.

    2004-12-28

    A video microscopic visualization system and image processing and data extraction and processing method for in situ detailed quantification of the deposition of sub-micrometer particles onto an arbitrary surface and determination of their concentration across the bulk suspension. The extracted data includes (a) surface concentration and flux of deposited, attached and detached colloids, (b) surface concentration and flux of arriving and departing colloids, (c) distribution of colloids in the bulk suspension in the direction perpendicular to the deposition surface, and (d) spatial and temporal distributions of deposited colloids.

  7. Autonomous image data reduction by analysis and interpretation. [for space missions

    NASA Technical Reports Server (NTRS)

    Eberlein, Susan; Yates, Gigi; Ritter, Niles

    1988-01-01

    Image data is a critical component of the scientific information acquired by space missions. Compression of image data is required due to the limited bandwidth of the data transmission channel and limited memory space on the acquisition vehicle. This need becomes more pressing when dealing with multispectral data where each pixel may comprise 300 or more bytes. An autonomous, real time, on-board image analysis system for an exploratory vehicle such as a Mars Rover is developed. The completed system will be capable of interpreting image data to produce reduced representations of the image, and of making decisions regarding the importance of data based on current scientific goals. Data from multiple sources, including stereo images, color images, and multispectral data, are fused into single image representations. Analysis techniques emphasize artificial neural networks. Clusters are described by their outlines and class values. These analysis and compression techniques are coupled with decision-making capacity for determining importance of each image region. Areas determined to be noise or uninteresting can be discarded in favor of more important areas. Thus limited resources for data storage and transmission are allocated to the most significant images.

  8. A distributed microcomputer-controlled system for data acquisition and power spectral analysis of EEG.

    PubMed

    Vo, T D; Dwyer, G; Szeto, H H

    1986-04-01

    A relatively powerful and inexpensive microcomputer-based system for the spectral analysis of the EEG is presented. High resolution and speed is achieved with the use of recently available large-scale integrated circuit technology with enhanced functionality (INTEL Math co-processors 8087) which can perform transcendental functions rapidly. The versatility of the system is achieved with a hardware organization that has distributed data acquisition capability performed by the use of a microprocessor-based analog to digital converter with large resident memory (Cyborg ISAAC-2000). Compiled BASIC programs and assembly language subroutines perform on-line or off-line the fast Fourier transform and spectral analysis of the EEG which is stored as soft as well as hard copy. Some results obtained from test application of the entire system in animal studies are presented.

  9. Automated eXpert Spectral Image Analysis

    SciTech Connect

    Keenan, Michael R.

    2003-11-25

    AXSIA performs automated factor analysis of hyperspectral images. In such images, a complete spectrum is collected an each point in a 1-, 2- or 3- dimensional spatial array. One of the remaining obstacles to adopting these techniques for routine use is the difficulty of reducing the vast quantities of raw spectral data to meaningful information. Multivariate factor analysis techniques have proven effective for extracting the essential information from high dimensional data sets into a limted number of factors that describe the spectral characteristics and spatial distributions of the pure components comprising the sample. AXSIA provides tools to estimate different types of factor models including Singular Value Decomposition (SVD), Principal Component Analysis (PCA), PCA with factor rotation, and Alternating Least Squares-based Multivariate Curve Resolution (MCR-ALS). As part of the analysis process, AXSIA can automatically estimate the number of pure components that comprise the data and can scale the data to account for Poisson noise. The data analysis methods are fundamentally based on eigenanalysis of the data crossproduct matrix coupled with orthogonal eigenvector rotation and constrained alternating least squares refinement. A novel method for automatically determining the number of significant components, which is based on the eigenvalues of the crossproduct matrix, has also been devised and implemented. The data can be compressed spectrally via PCA and spatially through wavelet transforms, and algorithms have been developed that perform factor analysis in the transform domain while retaining full spatial and spectral resolution in the final result. These latter innovations enable the analysis of larger-than core-memory spectrum-images. AXSIA was designed to perform automated chemical phase analysis of spectrum-images acquired by a variety of chemical imaging techniques. Successful applications include Energy Dispersive X-ray Spectroscopy, X-ray Fluorescence

  10. Objective facial photograph analysis using imaging software.

    PubMed

    Pham, Annette M; Tollefson, Travis T

    2010-05-01

    Facial analysis is an integral part of the surgical planning process. Clinical photography has long been an invaluable tool in the surgeon's practice not only for accurate facial analysis but also for enhancing communication between the patient and surgeon, for evaluating postoperative results, for medicolegal documentation, and for educational and teaching opportunities. From 35-mm slide film to the digital technology of today, clinical photography has benefited greatly from technological advances. With the development of computer imaging software, objective facial analysis becomes easier to perform and less time consuming. Thus, while the original purpose of facial analysis remains the same, the process becomes much more efficient and allows for some objectivity. Although clinical judgment and artistry of technique is never compromised, the ability to perform objective facial photograph analysis using imaging software may become the standard in facial plastic surgery practices in the future.

  11. Integral imaging acquisition and processing for visualization of photon counting images in the mid-wave infrared range

    NASA Astrophysics Data System (ADS)

    Latorre-Carmona, P.; Pla, F.; Javidi, B.

    2016-06-01

    In this paper, we present an overview of our previously published work on the application of the maximum likelihood (ML) reconstruction method to integral images acquired with a mid-wave infrared detector on two different types of scenes: one of them consisting of a road, a group of trees and a vehicle just behind one of the trees (being the car at a distance of more than 200m from the camera), and another one consisting of a view of the Wright Air Force Base airfield, with several hangars and different other types of installations (including warehouses) at distances ranging from 600m to more than 2km. Dark current noise is considered taking into account the particular features this type of sensors have. Results show that this methodology allows to improve visualization in the photon counting domain.

  12. Lexical Link Analysis Application: Improving Web Service to Acquisition Visibility Portal

    DTIC Science & Technology

    2014-05-01

    and performance data of selected acquisition reports (SAR) • Acquisition Strategy Reports ( ASR ) • … Gaps and Inconsistencies of AVP Data Sources...Test & Evaluation Master Plans (TEMP) – The acquisition community looks at the Acquisition Strategy Reports ( ASR ). • Milestone documents – Divergent...Inconsistencies among these documents may reflect a risk to the program. Where Do the Gaps Come From? (e.g. Compare ASR and TEMP) • What are the features or

  13. Monitoring of historical frescoes by timed infrared imaging analysis

    NASA Astrophysics Data System (ADS)

    Cadelano, G.; Bison, P.; Bortolin, A.; Ferrarini, G.; Peron, F.; Girotto, M.; Volinia, M.

    2015-03-01

    The subflorescence and efflorescence phenomena are widely acknowledged as the major causes of permanent damage to fresco wall paintings. They are related to the occurrence of cycles of dry/wet conditions inside the walls. Therefore, it is essential to identify the presence of water on the decorated surfaces and inside the walls. Nondestructive testing in industrial applications have confirmed that active infrared thermography with continuous timed images acquisition can improve the outcomes of thermal analysis aimed to moisture identification. In spite of that, in cultural heritage investigations these techniques have not been yet used extensively on a regular basis. This paper illustrates an application of these principles in order to evaluate the decay of fresco mural paintings in a medieval chapel located in North-West of Italy. One important feature of this study is the use of a robotic system called aIRview that can be utilized to automatically acquire and process thermal images. Multiple accurate thermal views of the inside walls of the building have been produced in a survey that lasted several days. Signal processing algorithms based on Fast Fourier Transform analysis have been applied to the acquired data in order to formulate trustworthy hypotheses about the deterioration mechanisms.

  14. Dynamic Chest Image Analysis: Evaluation of Model-Based Pulmonary Perfusion Analysis With Pyramid Images

    DTIC Science & Technology

    2007-11-02

    Image Analysis aims to develop model-based computer analysis and visualization methods for showing focal and general abnormalities of lung ventilation and perfusion based on a sequence of digital chest fluoroscopy frames collected with the Dynamic Pulmonary Imaging technique 18,5,17,6. We have proposed and evaluated a multiresolutional method with an explicit ventilation model based on pyramid images for ventilation analysis. We have further extended the method for ventilation analysis to pulmonary perfusion. This paper focuses on the clinical evaluation of our method for

  15. A standardised protocol for texture feature analysis of endoscopic images in gynaecological cancer

    PubMed Central

    Neofytou, Marios S; Tanos, Vasilis; Pattichis, Marios S; Pattichis, Constantinos S; Kyriacou, Efthyvoulos C; Koutsouris, Dimitris D

    2007-01-01

    Background In the development of tissue classification methods, classifiers rely on significant differences between texture features extracted from normal and abnormal regions. Yet, significant differences can arise due to variations in the image acquisition method. For endoscopic imaging of the endometrium, we propose a standardized image acquisition protocol to eliminate significant statistical differences due to variations in: (i) the distance from the tissue (panoramic vs close up), (ii) difference in viewing angles and (iii) color correction. Methods We investigate texture feature variability for a variety of targets encountered in clinical endoscopy. All images were captured at clinically optimum illumination and focus using 720 × 576 pixels and 24 bits color for: (i) a variety of testing targets from a color palette with a known color distribution, (ii) different viewing angles, (iv) two different distances from a calf endometrial and from a chicken cavity. Also, human images from the endometrium were captured and analysed. For texture feature analysis, three different sets were considered: (i) Statistical Features (SF), (ii) Spatial Gray Level Dependence Matrices (SGLDM), and (iii) Gray Level Difference Statistics (GLDS). All images were gamma corrected and the extracted texture feature values were compared against the texture feature values extracted from the uncorrected images. Statistical tests were applied to compare images from different viewing conditions so as to determine any significant differences. Results For the proposed acquisition procedure, results indicate that there is no significant difference in texture features between the panoramic and close up views and between angles. For a calibrated target image, gamma correction provided an acquired image that was a significantly better approximation to the original target image. In turn, this implies that the texture features extracted from the corrected images provided for better approximations

  16. Hormonal Contraception and the Risk of HIV Acquisition: An Individual Participant Data Meta-analysis

    PubMed Central

    Morrison, Charles S.; Chen, Pai-Lien; Kwok, Cynthia; Baeten, Jared M.; Brown, Joelle; Crook, Angela M.; Van Damme, Lut; Delany-Moretlwe, Sinead; Francis, Suzanna C.; Friedland, Barbara A.; Hayes, Richard J.; Heffron, Renee; Kapiga, Saidi; Karim, Quarraisha Abdool; Karpoff, Stephanie; Kaul, Rupert; McClelland, R. Scott; McCormack, Sheena; McGrath, Nuala; Myer, Landon; Rees, Helen; van der Straten, Ariane; Watson-Jones, Deborah; van de Wijgert, Janneke H. H. M.; Stalter, Randy; Low, Nicola

    2015-01-01

    Background Observational studies of a putative association between hormonal contraception (HC) and HIV acquisition have produced conflicting results. We conducted an individual participant data (IPD) meta-analysis of studies from sub-Saharan Africa to compare the incidence of HIV infection in women using combined oral contraceptives (COCs) or the injectable progestins depot-medroxyprogesterone acetate (DMPA) or norethisterone enanthate (NET-EN) with women not using HC. Methods and Findings Eligible studies measured HC exposure and incident HIV infection prospectively using standardized measures, enrolled women aged 15–49 y, recorded ≥15 incident HIV infections, and measured prespecified covariates. Our primary analysis estimated the adjusted hazard ratio (aHR) using two-stage random effects meta-analysis, controlling for region, marital status, age, number of sex partners, and condom use. We included 18 studies, including 37,124 women (43,613 woman-years) and 1,830 incident HIV infections. Relative to no HC use, the aHR for HIV acquisition was 1.50 (95% CI 1.24–1.83) for DMPA use, 1.24 (95% CI 0.84–1.82) for NET-EN use, and 1.03 (95% CI 0.88–1.20) for COC use. Between-study heterogeneity was mild (I2 < 50%). DMPA use was associated with increased HIV acquisition compared with COC use (aHR 1.43, 95% CI 1.23–1.67) and NET-EN use (aHR 1.32, 95% CI 1.08–1.61). Effect estimates were attenuated for studies at lower risk of methodological bias (compared with no HC use, aHR for DMPA use 1.22, 95% CI 0.99–1.50; for NET-EN use 0.67, 95% CI 0.47–0.96; and for COC use 0.91, 95% CI 0.73–1.41) compared to those at higher risk of bias (pinteraction = 0.003). Neither age nor herpes simplex virus type 2 infection status modified the HC–HIV relationship. Conclusions This IPD meta-analysis found no evidence that COC or NET-EN use increases women’s risk of HIV but adds to the evidence that DMPA may increase HIV risk, underscoring the need for additional safe

  17. TU-F-CAMPUS-I-02: Contrast Enhanced Cone Beam CT Imaging with Dual- Gantry Image Acquisition and Constrained Iterative Reconstruction-a Simulation Study for Liver Imaging Application

    SciTech Connect

    Zhong, Y; Gupta, S; Lai, C; Wang, T; Shaw, C

    2015-06-15

    Purpose: Contrast time-density curves may help differentiate malignant tumors from normal tissues or benign tumors. Repetitive scans using conventional CT or cone beam CT techniques, which Result in unacceptably high dose, may not achieve the desired temporal resolution. In this study we describe and demonstrate a 4D imaging technique for imaging and quantifying contrast flows requiring only one or two 360° scans. Methods: A dual-gantry system is used to simultaneously acquire two projection images at orthogonal orientations. Following the scan, each or both of the two 360° projection sets are used to reconstruct an average contrast enhanced image set which is then segmented to form a 3D contrast map. Alternatively, a pre-injection scan may be made and used to reconstruct a pre-injection image set which is subtracted from the post-injection image set to form the 3D contrast map. Each of the two 360° projection sets is divided into 12 subsets, thus creating 12 pairs of 30° limited angle projection sets, each corresponding to a time spanning over 1/12 of the scanning time. Each pair of the projection sets are reconstructed as a time specific 3D image set with the maximum likelihood estimation iterative algorithm using the contrast map as the constraint. As a demonstration, a 4D abdominal phantom was constructed from clinical CT images with blood flow through the normal tissue and a tumor modeled and imaging process simulated. Results: We have successfully generated a 4D image phantom, and calculated the projection images. The time density curves derived from the reconstructed image set matched well with the flow model used to generate the phantom. Conclusion: Dual-gantry image acquisition and constrained iterative reconstruction algorithm may help to obtain time-density curves of contrast agents in blood flows, which may help differentiate malignant tumors from normal tissues or benign tumors.

  18. Motion Analysis From Television Images

    NASA Astrophysics Data System (ADS)

    Silberberg, George G.; Keller, Patrick N.

    1982-02-01

    The Department of Defense ranges have relied on photographic instrumentation for gathering data of firings for all types of ordnance. A large inventory of cameras are available on the market that can be used for these tasks. A new set of optical instrumentation is beginning to appear which, in many cases, can directly replace photographic cameras for a great deal of the work being performed now. These are television cameras modified so they can stop motion, see in the dark, perform under hostile environments, and provide real time information. This paper discusses techniques for modifying television cameras so they can be used for motion analysis.

  19. Metabolome Analysis of Arabidopsis thaliana Roots Identifies a Key Metabolic Pathway for Iron Acquisition

    PubMed Central

    Schmidt, Holger; Günther, Carmen; Weber, Michael; Spörlein, Cornelia; Loscher, Sebastian; Böttcher, Christoph; Schobert, Rainer; Clemens, Stephan

    2014-01-01

    Fe deficiency compromises both human health and plant productivity. Thus, it is important to understand plant Fe acquisition strategies for the development of crop plants which are more Fe-efficient under Fe-limited conditions, such as alkaline soils, and have higher Fe density in their edible tissues. Root secretion of phenolic compounds has long been hypothesized to be a component of the reduction strategy of Fe acquisition in non-graminaceous plants. We therefore subjected roots of Arabidopsis thaliana plants grown under Fe-replete and Fe-deplete conditions to comprehensive metabolome analysis by gas chromatography-mass spectrometry and ultra-pressure liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry. Scopoletin and other coumarins were found among the metabolites showing the strongest response to two different Fe-limited conditions, the cultivation in Fe-free medium and in medium with an alkaline pH. A coumarin biosynthesis mutant defective in ortho-hydroxylation of cinnamic acids was unable to grow on alkaline soil in the absence of Fe fertilization. Co-cultivation with wild-type plants partially rescued the Fe deficiency phenotype indicating a contribution of extracellular coumarins to Fe solubilization. Indeed, coumarins were detected in root exudates of wild-type plants. Direct infusion mass spectrometry as well as UV/vis spectroscopy indicated that coumarins are acting both as reductants of Fe(III) and as ligands of Fe(II). PMID:25058345

  20. Metabolome analysis of Arabidopsis thaliana roots identifies a key metabolic pathway for iron acquisition.

    PubMed

    Schmidt, Holger; Günther, Carmen; Weber, Michael; Spörlein, Cornelia; Loscher, Sebastian; Böttcher, Christoph; Schobert, Rainer; Clemens, Stephan

    2014-01-01

    Fe deficiency compromises both human health and plant productivity. Thus, it is important to understand plant Fe acquisition strategies for the development of crop plants which are more Fe-efficient under Fe-limited conditions, such as alkaline soils, and have higher Fe density in their edible tissues. Root secretion of phenolic compounds has long been hypothesized to be a component of the reduction strategy of Fe acquisition in non-graminaceous plants. We therefore subjected roots of Arabidopsis thaliana plants grown under Fe-replete and Fe-deplete conditions to comprehensive metabolome analysis by gas chromatography-mass spectrometry and ultra-pressure liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry. Scopoletin and other coumarins were found among the metabolites showing the strongest response to two different Fe-limited conditions, the cultivation in Fe-free medium and in medium with an alkaline pH. A coumarin biosynthesis mutant defective in ortho-hydroxylation of cinnamic acids was unable to grow on alkaline soil in the absence of Fe fertilization. Co-cultivation with wild-type plants partially rescued the Fe deficiency phenotype indicating a contribution of extracellular coumarins to Fe solubilization. Indeed, coumarins were detected in root exudates of wild-type plants. Direct infusion mass spectrometry as well as UV/vis spectroscopy indicated that coumarins are acting both as reductants of Fe(III) and as ligands of Fe(II).

  1. Multilocus Genetic Analysis of Brain Images

    PubMed Central

    Hibar, Derrek P.; Kohannim, Omid; Stein, Jason L.; Chiang, Ming-Chang; Thompson, Paul M.

    2011-01-01

    The quest to identify genes that influence disease is now being extended to find genes that affect biological markers of disease, or endophenotypes. Brain images, in particular, provide exquisitely detailed measures of anatomy, function, and connectivity in the living brain, and have identified characteristic features for many neurological and psychiatric disorders. The emerging field of imaging genomics is discovering important genetic variants associated with brain structure and function, which in turn influence disease risk and fundamental cognitive processes. Statistical approaches for testing genetic associations are not straightforward to apply to brain images because the data in brain images is spatially complex and generally high dimensional. Neuroimaging phenotypes typically include 3D maps across many points in the brain, fiber tracts, shape-based analyses, and connectivity matrices, or networks. These complex data types require new methods for data reduction and joint consideration of the image and the genome. Image-wide, genome-wide searches are now feasible, but they can be greatly empowered by sparse regression or hierarchical clustering methods that isolate promising features, boosting statistical power. Here we review the evolution of statistical approaches to assess genetic influences on the brain. We outline the current state of multivariate statistics in imaging genomics, and future directions, including meta-analysis. We emphasize the power of novel multivariate approaches to discover reliable genetic influences with small effect sizes. PMID:22303368

  2. Medical image analysis with artificial neural networks.

    PubMed

    Jiang, J; Trundle, P; Ren, J

    2010-12-01

    Given that neural networks have been widely reported in the research community of medical imaging, we provide a focused literature survey on recent neural network developments in computer-aided diagnosis, medical image segmentation and edge detection towards visual content analysis, and medical image registration for its pre-processing and post-processing, with the aims of increasing awareness of how neural networks can be applied to these areas and to provide a foundation for further research and practical development. Representative techniques and algorithms are explained in detail to provide inspiring examples illustrating: (i) how a known neural network with fixed structure and training procedure could be applied to resolve a medical imaging problem; (ii) how medical images could be analysed, processed, and characterised by neural networks; and (iii) how neural networks could be expanded further to resolve problems relevant to medical imaging. In the concluding section, a highlight of comparisons among many neural network applications is included to provide a global view on computational intelligence with neural networks in medical imaging.

  3. Analysis of Source Selection Methods and Performance Outcomes: Lowest Price Technically Acceptable vs. Tradeoff in Air Force Acquisitions

    DTIC Science & Technology

    2015-12-01

    NAVAL POSTGRADUATE SCHOOL MONTEREY, CALIFORNIA MBA PROFESSIONAL REPORT ANALYSIS OF SOURCE SELECTION METHODS AND PERFORMANCE...2. REPORT DATE December 2015 3. REPORT TYPE AND DATES COVERED MBA Professional Report 4. TITLE AND SUBTITLE ANALYSIS OF SOURCE SELECTION METHODS AND...distribution is unlimited 12b. DISTRIBUTION CODE 13. ABSTRACT (maximum 200 words) As part of procurement planning, government acquisition teams must select

  4. Curvelet Based Offline Analysis of SEM Images

    PubMed Central

    Shirazi, Syed Hamad; Haq, Nuhman ul; Hayat, Khizar; Naz, Saeeda; Haque, Ihsan ul

    2014-01-01

    Manual offline analysis, of a scanning electron microscopy (SEM) image, is a time consuming process and requires continuous human intervention and efforts. This paper presents an image processing based method for automated offline analyses of SEM images. To this end, our strategy relies on a two-stage process, viz. texture analysis and quantification. The method involves a preprocessing step, aimed at the noise removal, in order to avoid false edges. For texture analysis, the proposed method employs a state of the art Curvelet transform followed by segmentation through a combination of entropy filtering, thresholding and mathematical morphology (MM). The quantification is carried out by the application of a box-counting algorithm, for fractal dimension (FD) calculations, with the ultimate goal of measuring the parameters, like surface area and perimeter. The perimeter is estimated indirectly by counting the boundary boxes of the filled shapes. The proposed method, when applied to a representative set of SEM images, not only showed better results in image segmentation but also exhibited a good accuracy in the calculation of surface area and perimeter. The proposed method outperforms the well-known Watershed segmentation algorithm. PMID:25089617

  5. Fourier analysis: from cloaking to imaging

    NASA Astrophysics Data System (ADS)

    Wu, Kedi; Cheng, Qiluan; Wang, Guo Ping

    2016-04-01

    Regarding invisibility cloaks as an optical imaging system, we present a Fourier approach to analytically unify both Pendry cloaks and complementary media-based invisibility cloaks into one kind of cloak. By synthesizing different transfer functions, we can construct different devices to realize a series of interesting functions such as hiding objects (events), creating illusions, and performing perfect imaging. In this article, we give a brief review on recent works of applying Fourier approach to analysis invisibility cloaks and optical imaging through scattering layers. We show that, to construct devices to conceal an object, no constructive materials with extreme properties are required, making most, if not all, of the above functions realizable by using naturally occurring materials. As instances, we experimentally verify a method of directionally hiding distant objects and create illusions by using all-dielectric materials, and further demonstrate a non-invasive method of imaging objects completely hidden by scattering layers.

  6. Deep Learning in Medical Image Analysis.

    PubMed

    Shen, Dinggang; Wu, Guorong; Suk, Heung-Il

    2017-03-09

    This review covers computer-assisted analysis of images in the field of medical imaging. Recent advances in machine learning, especially with regard to deep learning, are helping to identify, classify, and quantify patterns in medical images. At the core of these advances is the ability to exploit hierarchical feature representations learned solely from data, instead of features designed by hand according to domain-specific knowledge. Deep learning is rapidly becoming the state of the art, leading to enhanced performance in various medical applications. We introduce the fundamentals of deep learning methods and review their successes in image registration, detection of anatomical and cellular structures, tissue segmentation, computer-aided disease diagnosis and prognosis, and so on. We conclude by discussing research issues and suggesting future directions for further improvement. Expected final online publication date for the Annual Review of Biomedical Engineering Volume 19 is June 4, 2017. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

  7. Ultra-low dose CT attenuation correction for PET/CT: analysis of sparse view data acquisition and reconstruction algorithms

    NASA Astrophysics Data System (ADS)

    Rui, Xue; Cheng, Lishui; Long, Yong; Fu, Lin; Alessio, Adam M.; Asma, Evren; Kinahan, Paul E.; De Man, Bruno

    2015-09-01

    For PET/CT systems, PET image reconstruction requires corresponding CT images for anatomical localization and attenuation correction. In the case of PET respiratory gating, multiple gated CT scans can offer phase-matched attenuation and motion correction, at the expense of increased radiation dose. We aim to minimize the dose of the CT scan, while preserving adequate image quality for the purpose of PET attenuation correction by introducing sparse view CT data acquisition. We investigated sparse view CT acquisition protocols resulting in ultra-low dose CT scans designed for PET attenuation correction. We analyzed the tradeoffs between the number of views and the integrated tube current per view for a given dose using CT and PET simulations of a 3D NCAT phantom with lesions inserted into liver and lung. We simulated seven CT acquisition protocols with {984, 328, 123, 41, 24, 12, 8} views per rotation at a gantry speed of 0.35 s. One standard dose and four ultra-low dose levels, namely, 0.35 mAs, 0.175 mAs, 0.0875 mAs, and 0.043 75 mAs, were investigated. Both the analytical Feldkamp, Davis and Kress (FDK) algorithm and the Model Based Iterative Reconstruction (MBIR) algorithm were used for CT image reconstruction. We also evaluated the impact of sinogram interpolation to estimate the missing projection measurements due to sparse view data acquisition. For MBIR, we used a penalized weighted least squares (PWLS) cost function with an approximate total-variation (TV) regularizing penalty function. We compared a tube pulsing mode and a continuous exposure mode for sparse view data acquisition. Global PET ensemble root-mean-squares-error (RMSE) and local ensemble lesion activity error were used as quantitative evaluation metrics for PET image quality. With sparse view sampling, it is possible to greatly reduce the CT scan dose when it is primarily used for PET attenuation correction with little or no measureable effect on the PET image. For the four ultra-low dose

  8. Ultra-low dose CT attenuation correction for PET/CT: analysis of sparse view data acquisition and reconstruction algorithms.

    PubMed

    Rui, Xue; Cheng, Lishui; Long, Yong; Fu, Lin; Alessio, Adam M; Asma, Evren; Kinahan, Paul E; De Man, Bruno

    2015-10-07

    For PET/CT systems, PET image reconstruction requires corresponding CT images for anatomical localization and attenuation correction. In the case of PET respiratory gating, multiple gated CT scans can offer phase-matched attenuation and motion correction, at the expense of increased radiation dose. We aim to minimize the dose of the CT scan, while preserving adequate image quality for the purpose of PET attenuation correction by introducing sparse view CT data acquisition.We investigated sparse view CT acquisition protocols resulting in ultra-low dose CT scans designed for PET attenuation correction. We analyzed the tradeoffs between the number of views and the integrated tube current per view for a given dose using CT and PET simulations of a 3D NCAT phantom with lesions inserted into liver and lung. We simulated seven CT acquisition protocols with {984, 328, 123, 41, 24, 12, 8} views per rotation at a gantry speed of 0.35 s. One standard dose and four ultra-low dose levels, namely, 0.35 mAs, 0.175 mAs, 0.0875 mAs, and 0.043 75 mAs, were investigated. Both the analytical Feldkamp, Davis and Kress (FDK) algorithm and the Model Based Iterative Reconstruction (MBIR) algorithm were used for CT image reconstruction. We also evaluated the impact of sinogram interpolation to estimate the missing projection measurements due to sparse view data acquisition. For MBIR, we used a penalized weighted least squares (PWLS) cost function with an approximate total-variation (TV) regularizing penalty function. We compared a tube pulsing mode and a continuous exposure mode for sparse view data acquisition. Global PET ensemble root-mean-squares-error (RMSE) and local ensemble lesion activity error were used as quantitative evaluation metrics for PET image quality.With sparse view sampling, it is possible to greatly reduce the CT scan dose when it is primarily used for PET attenuation correction with little or no measureable effect on the PET image. For the four ultra-low dose levels

  9. A new set of 299 pictures for psycholinguistic studies: French norms for name agreement, image agreement, conceptual familiarity, visual complexity, image variability, age of acquisition, and naming latencies.

    PubMed

    Bonin, Patrick; Peereman, Ronald; Malardier, Nathalie; Méot, Alain; Chalard, Marylène

    2003-02-01

    Pictures are often used as stimuli in studies of perception, language, and memory. Since performances on different sets of pictures are generally contrasted, stimulus selection requires the use of standardized material to match pictures across different variables. Unfortunately, the number of standardized pictures available for empirical research is rather limited. The aim of the present study is to provide French normative data for a new set of 299 black-and-white drawings. Alario and Ferrand (1999) were closely followed in that the pictures were standardized on six variables name agreement, image agreement, conceptual familiarity, visual complexity, image variability, and age of acquisition. Objective frequency measures are also provided for themost common names associated with the pictures. Comparative analyses between our results and the norms obtained in other, similar studies are reported. Finally, naming latencies corresponding to the set of pictures were also collected from French native speakers, and correlational/multiple-regression analyses were performed on naming latencies. This new set of standardized pictures is available on the Internet (http://leadserv.u-bourgogne.fr/bases/pictures/) and should be of great use to researchers when they select pictorial stimuli.

  10. Positron Emission Mammography with Multiple Angle Acquisition

    SciTech Connect

    Mark F. Smith; Stan Majewski; Raymond R. Raylman

    2002-11-01

    Positron emission mammography (PEM) of F-18 fluorodeoxyglucose (FDG) uptake in breast tumors with dedicated detectors typically has been accomplished with two planar detectors in a fixed position with the breast under compression. The potential use of PEM imaging at two detector positions to guide stereotactic breast biopsy has motivated us to use PEM coincidence data acquired at two or more detector positions together in a single image reconstruction. Multiple angle PEM acquisition and iterative image reconstruction were investigated using point source and compressed breast phantom acquisitions with 5, 9, 12 and 15 mm diameter spheres and a simulated tumor:background activity concentration ratio of 6:1. Image reconstruction was performed with an iterative MLEM algorithm that used coincidence events between any two detector pixels on opposed detector heads at each detector position. This present study compared two acquisition protocols: 2 angle acquisition with detector angular positions of -15 and +15 degrees and 11 angle acquisition with detector positions spaced at 3 degree increments over the range -15 to +15 degrees. Three- dimensional image resolution was assessed for the point source acquisitions, and contrast and signal-to-noise metrics were evaluated for the compressed breast phantom with different simulated tumor sizes. Radial and tangential resolutions were similar for the two protocols, while normal resolution was better for the 2 angle acquisition. Analysis is complicated by the asymmetric point spread functions. Signal- to-noise vs. contrast tradeoffs were better for 11 angle acquisition for the smallest visible 9 mm sphere, while tradeoff results were mixed for the larger and more easily visible 12 mm and 15 mm diameter spheres. Additional study is needed to better understand the performance of limited angle tomography for PEM. PEM tomography experiments with complete angular sampling are planned.

  11. Positron Emission Mammography with Multiple Angle Acquisition

    SciTech Connect

    Mark F. Smith; Stan Majewski; Raymond R. Raylman

    2002-11-01

    Positron emission mammography (PEM) of F-18 fluorodeoxyglucose (FbG) uptake in breast tumors with dedicated detectors typically has been accomplished with two planar detectors in a fixed position with the breast under compression. The potential use of PEM imaging at two detector positions to guide stereotactic breast biopsy has motivated us to use PEM coincidence data acquired at two or more detector positions together in a single image reconstruction. Multiple angle PEM acquisition and iterative image reconstruction were investigated using point source and compressed breast phantom acquisitions with 5, 9, 12 and 15 mm diameter spheres and a simulated tumor:background activity concentration ratio of 6:1. Image reconstruction was performed with an iterative MLEM algorithm that used coincidence events between any two detector pixels on opposed detector heads at each detector position. This present study compared two acquisition protocols: 2 angle acquisition with detector angular positions of -15 and +15 degrees and 11 angle acquisition with detector positions spaced at 3 degree increments over the range -15 to +15 degrees. Three-dimensional image resolution was assessed for the point source acquisitions, and contrast and signal-to-noise metrics were evaluated for the compressed breast phantom with different simulated tumor sizes. Radial and tangential resolutions were similar for the two protocols, while normal resolution was better for the 2 angle acquisition. Analysis is complicated by the asymmetric point spread functions. Signal- to-noise vs. contrast tradeoffs were better for 11 angle acquisition for the smallest visible 9 mm sphere, while tradeoff results were mixed for the larger and more easily visible 12 mm and 15 mm diameter spheres. Additional study is needed to better understand the performance of limited angle tomography for PEM. PEM tomography experiments with complete angular sampling are planned.

  12. Measuring toothbrush interproximal penetration using image analysis

    NASA Astrophysics Data System (ADS)

    Hayworth, Mark S.; Lyons, Elizabeth K.

    1994-09-01

    An image analysis method of measuring the effectiveness of a toothbrush in reaching the interproximal spaces of teeth is described. Artificial teeth are coated with a stain that approximates real plaque and then brushed with a toothbrush on a brushing machine. The teeth are then removed and turned sideways so that the interproximal surfaces can be imaged. The areas of stain that have been removed within masked regions that define the interproximal regions are measured and reported. These areas correspond to the interproximal areas of the tooth reached by the toothbrush bristles. The image analysis method produces more precise results (10-fold decrease in standard deviation) in a fraction (22%) of the time as compared to our prior visual grading method.

  13. Dual CARS and SHG image acquisition scheme that combines single central fiber and multimode fiber bundle to collect and differentiate backward and forward generated photons

    PubMed Central

    Weng, Sheng; Chen, Xu; Xu, Xiaoyun; Wong, Kelvin K.; Wong, Stephen T. C.

    2016-01-01

    In coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) imaging, backward and forward generated photons exhibit different image patterns and thus capture salient intrinsic information of tissues from different perspectives. However, they are often mixed in collection using traditional image acquisition methods and thus are hard to interpret. We developed a multimodal scheme using a single central fiber and multimode fiber bundle to simultaneously collect and differentiate images formed by these two types of photons and evaluated the scheme in an endomicroscopy prototype. The ratio of these photons collected was calculated for the characterization of tissue regions with strong or weak epi-photon generation while different image patterns of these photons at different tissue depths were revealed. This scheme provides a new approach to extract and integrate information captured by backward and forward generated photons in dual CARS/SHG imaging synergistically for biomedical applications. PMID:27375938

  14. Dual CARS and SHG image acquisition scheme that combines single central fiber and multimode fiber bundle to collect and differentiate backward and forward generated photons.

    PubMed

    Weng, Sheng; Chen, Xu; Xu, Xiaoyun; Wong, Kelvin K; Wong, Stephen T C

    2016-06-01

    In coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) imaging, backward and forward generated photons exhibit different image patterns and thus capture salient intrinsic information of tissues from different perspectives. However, they are often mixed in collection using traditional image acquisition methods and thus are hard to interpret. We developed a multimodal scheme using a single central fiber and multimode fiber bundle to simultaneously collect and differentiate images formed by these two types of photons and evaluated the scheme in an endomicroscopy prototype. The ratio of these photons collected was calculated for the characterization of tissue regions with strong or weak epi-photon generation while different image patterns of these photons at different tissue depths were revealed. This scheme provides a new approach to extract and integrate information captured by backward and forward generated photons in dual CARS/SHG imaging synergistically for biomedical applications.

  15. Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis.

    PubMed

    Peng, Hanchuan; Tang, Jianyong; Xiao, Hang; Bria, Alessandro; Zhou, Jianlong; Butler, Victoria; Zhou, Zhi; Gonzalez-Bellido, Paloma T; Oh, Seung W; Chen, Jichao; Mitra, Ananya; Tsien, Richard W; Zeng, Hongkui; Ascoli, Giorgio A; Iannello, Giulio; Hawrylycz, Michael; Myers, Eugene; Long, Fuhui

    2014-07-11

    Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain.

  16. Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

    PubMed Central

    Peng, Hanchuan; Tang, Jianyong; Xiao, Hang; Bria, Alessandro; Zhou, Jianlong; Butler, Victoria; Zhou, Zhi; Gonzalez-Bellido, Paloma T.; Oh, Seung W.; Chen, Jichao; Mitra, Ananya; Tsien, Richard W.; Zeng, Hongkui; Ascoli, Giorgio A.; Iannello, Giulio; Hawrylycz, Michael; Myers, Eugene; Long, Fuhui

    2014-01-01

    Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain. PMID:25014658

  17. An approach to automatic blood vessel image registration of microcirculation for blood flow analysis on nude mice.

    PubMed

    Lin, Wen-Chen; Wu, Chih-Chieh; Zhang, Geoffrey; Wu, Tung-Hsin; Lin, Yang-Hsien; Huang, Tzung-Chi; Liu, Ren-Shyan; Lin, Kang-Ping

    2011-04-01

    Image registration is often a required and a time-consuming step in blood flow analysis of large microscopic video sequences in vivo. In order to obtain stable images for blood flow analysis, frame-to-frame image matching as a preprocessing step is a solution to the problem of movement during image acquisition. In this paper, microscopic system analysis without fluorescent labelling is performed to provide precise and continuous quantitative data of blood flow rate in individual microvessels of nude mice. The performance properties of several matching metrics are evaluated through simulated image registrations. An automatic image registration programme based on Powell's optimisation search method with low calculation redundancy was implemented. The matching method by variance of ratio is computationally efficient and improves the registration robustness and accuracy in practical application of microcirculation registration. The presented registration method shows acceptable results in close requisition to analyse red blood cell velocities, confirming the scientific potential of the system in blood flow analysis.

  18. Unsupervised hyperspectral image analysis using independent component analysis (ICA)

    SciTech Connec