Quantification of Lysine Acetylation and Succinylation Stoichiometry in Proteins Using Mass Spectrometric Data-Independent Acquisitions (SWATH)

DOE PAGES

Meyer, Jesse G.; D’Souza, Alexandria K.; Sorensen, Dylan J.; ...

2016-09-02

Post-translational modification of lysine residues by N ε-acylation is an important regulator of protein function. Many large-scale protein acylation studies have assessed relative changes of lysine acylation sites after antibody enrichment using mass spectrometry-based proteomics. Although relative acylation fold-changes are important, this does not reveal site occupancy, or stoichiometry, of individual modification sites, which is critical to understand functional consequences. Recently, methods for determining lysine acetylation stoichiometry have been proposed based on ratiometric analysis of endogenous levels to those introduced after quantitative per-acetylation of proteins using stable isotope-labeled acetic anhydride. However, in our hands, we find that these methods canmore » overestimate acetylation stoichiometries because of signal interferences when endogenous levels of acylation are very low, which is especially problematic when using MS1 scans for quantification. In this study, we sought to improve the accuracy of determining acylation stoichiometry using data-independent acquisition (DIA). Specifically, we use SWATH acquisition to comprehensively collect both precursor and fragment ion intensity data. The use of fragment ions for stoichiometry quantification not only reduces interferences but also allows for determination of site-level stoichiometry from peptides with multiple lysine residues. We also demonstrate the novel extension of this method to measurements of succinylation stoichiometry using deuterium-labeled succinic anhydride. Proof of principle SWATH acquisition studies were first performed using bovine serum albumin for both acetylation and succinylation occupancy measurements, followed by the analysis of more complex samples of E. coli cell lysates. Although overall site occupancy was low (<1%), some proteins contained lysines with relatively high acetylation occupancy.« less

Continuous Reusability using Immobilized HasApf in Chemoenzymatic Deracemization: A New Heterogeneous Enzyme Catalysis.

PubMed

Nagaoka, Hiroyuki

2016-10-25

This study found that the calibration curve of heme acquisition system A (HasA, a new reactive active species) immobilized by a porous ceramic particle (ImHApf; immobilized HasA from Pseudomonas fluorescens ) can be constructed in the range of 1750-1450 cm -1 using Fourier transform infrared spectroscopy (FTIR) analysis, and evaluated its catalytic efficiency. In the asymmetric oxidation of rac -1-(6-methoxynaphthalen-2-yl)ethanol ( rac - 1 : a naproxen precursor), a product ketone from the ( R )-isomer is desymmetrized using NaBH₄ and continuously reused even if treated with an organic solvent in 50 mM glycine-NaOH buffer at 40 °C in the absence of nicotinamide adenine dinucleotide (NAD(P)), leading to >99% enantiomeric excess and >90% chemical yield; the activity was calculated at 0.74 ± 0.03 mU/(mg·min) and the turnover number was determined to be approximately 2 × 10⁶. It was confirmed that the other sec -alcohols such as rac -1-(2-naphthyl)ethanol ( rac - 2 ) and m - and p -substituted rac -1-phenyl ethanols ( rac - 3ab - 6ab ) using ImHApf can also yield a single stereoisomer from a racemate. Therefore, HasA immobilization can be expected to become an important tool for building an environmentally friendly system that promotes industrial sustainability.

Arabinogalactan-protein secretion is associated with the acquisition of stigmatic receptivity in the apple flower

PubMed Central

Losada, Juan M.; Herrero, María

2012-01-01

Background and Aims Stigmatic receptivity plays a clear role in pollination dynamics; however, little is known about the factors that confer to a stigma the competence to be receptive for the germination of pollen grains. In this work, a developmental approach is used to evaluate the acquisition of stigmatic receptivity and its relationship with a possible change in arabinogalactan-proteins (AGPs). Methods Flowers of the domestic apple, Malus × domestica, were assessed for their capacity to support pollen germination at different developmental stages. Stigmas from these same stages were characterized morphologically and different AGP epitopes detected by immunocytochemistry. Key Results Acquisition of stigmatic receptivity and the secretion of classical AGPs from stigmatic cells occurred concurrently and following the same spatial distribution. While in unpollinated stigmas AGPs appeared unaltered, in cross-pollinated stigmas AGPs epitopes vanished as pollen tubes passed by. Conclusions The concurrent secretion of AGPs with the acquisition of stigmatic receptivity, together with the differential response in unpollinated and cross-pollinated pistils point out a role of AGPs in supporting pollen tube germination and strongly suggest that secretion of AGPs is associated with the acquisition of stigma receptivity. PMID:22652420

Evolution of photorespiration from cyanobacteria to land plants, considering protein phylogenies and acquisition of carbon concentrating mechanisms.

PubMed

Hagemann, Martin; Kern, Ramona; Maurino, Veronica G; Hanson, David T; Weber, Andreas P M; Sage, Rowan F; Bauwe, Hermann

2016-05-01

Photorespiration and oxygenic photosynthesis are intimately linked processes. It has been shown that under the present day atmospheric conditions cyanobacteria and all eukaryotic phototrophs need functional photorespiration to grow autotrophically. The question arises as to when this essential partnership evolved, i.e. can we assume a coevolution of both processes from the beginning or did photorespiration evolve later to compensate for the generation of 2-phosphoglycolate (2PG) due to Rubisco's oxygenase reaction? This question is mainly discussed here using phylogenetic analysis of proteins involved in the 2PG metabolism and the acquisition of different carbon concentrating mechanisms (CCMs). The phylogenies revealed that the enzymes involved in the photorespiration of vascular plants have diverse origins, with some proteins acquired from cyanobacteria as ancestors of the chloroplasts and others from heterotrophic bacteria as ancestors of mitochondria in the plant cell. Only phosphoglycolate phosphatase was found to originate from Archaea. Notably glaucophyte algae, the earliest branching lineage of Archaeplastida, contain more photorespiratory enzymes of cyanobacterial origin than other algal lineages or land plants indicating a larger initial contribution of cyanobacterial-derived proteins to eukaryotic photorespiration. The acquisition of CCMs is discussed as a proxy for assessing the timing of periods when photorespiratory activity may have been enhanced. The existence of CCMs also had marked influence on the structure and function of photorespiration. Here, we discuss evidence for an early and continuous coevolution of photorespiration, CCMs and photosynthesis starting from cyanobacteria via algae, to land plants. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

A relay network of extracellular heme-binding proteins drives C. albicans iron acquisition from hemoglobin.

PubMed

Kuznets, Galit; Vigonsky, Elena; Weissman, Ziva; Lalli, Daniela; Gildor, Tsvia; Kauffman, Sarah J; Turano, Paola; Becker, Jeffrey; Lewinson, Oded; Kornitzer, Daniel

2014-10-01

Iron scavenging constitutes a crucial challenge for survival of pathogenic microorganisms in the iron-poor host environment. Candida albicans, like many microbial pathogens, is able to utilize iron from hemoglobin, the largest iron pool in the host's body. Rbt5 is an extracellular glycosylphosphatidylinositol (GPI)-anchored heme-binding protein of the CFEM family that facilitates heme-iron uptake by an unknown mechanism. Here, we characterize an additional C. albicans CFEM protein gene, PGA7, deletion of which elicits a more severe heme-iron utilization phenotype than deletion of RBT5. The virulence of the pga7-/- mutant is reduced in a mouse model of systemic infection, consistent with a requirement for heme-iron utilization for C. albicans pathogenicity. The Pga7 and Rbt5 proteins exhibit distinct cell wall attachment, and discrete localization within the cell envelope, with Rbt5 being more exposed than Pga7. Both proteins are shown here to efficiently extract heme from hemoglobin. Surprisingly, while Pga7 has a higher affinity for heme in vitro, we find that heme transfer can occur bi-directionally between Pga7 and Rbt5, supporting a model in which they cooperate in a heme-acquisition relay. Together, our data delineate the roles of Pga7 and Rbt5 in a cell surface protein network that transfers heme from extracellular hemoglobin to the endocytic pathway, and provide a paradigm for how receptors embedded in the cell wall matrix can mediate nutrient uptake across the fungal cell envelope.

Application of wide selected-ion monitoring data-independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor

USDA-ARS?s Scientific Manuscript database

We describe here the use of label-free wide selected-ion monitoring data-independent acquisition (WiSIM-DIA) to identify proteins that are involved in the formation of tomato (Solanum lycopersicum) fruit cuticles and that are regulated by the transcription factor CUTIN DEFICIENT2 (CD2). A spectral l...

A Relay Network of Extracellular Heme-Binding Proteins Drives C. albicans Iron Acquisition from Hemoglobin

PubMed Central

Kuznets, Galit; Vigonsky, Elena; Weissman, Ziva; Lalli, Daniela; Gildor, Tsvia; Kauffman, Sarah J.; Turano, Paola; Becker, Jeffrey; Lewinson, Oded; Kornitzer, Daniel

2014-01-01

Iron scavenging constitutes a crucial challenge for survival of pathogenic microorganisms in the iron-poor host environment. Candida albicans, like many microbial pathogens, is able to utilize iron from hemoglobin, the largest iron pool in the host's body. Rbt5 is an extracellular glycosylphosphatidylinositol (GPI)-anchored heme-binding protein of the CFEM family that facilitates heme-iron uptake by an unknown mechanism. Here, we characterize an additional C. albicans CFEM protein gene, PGA7, deletion of which elicits a more severe heme-iron utilization phenotype than deletion of RBT5. The virulence of the pga7−/− mutant is reduced in a mouse model of systemic infection, consistent with a requirement for heme-iron utilization for C. albicans pathogenicity. The Pga7 and Rbt5 proteins exhibit distinct cell wall attachment, and discrete localization within the cell envelope, with Rbt5 being more exposed than Pga7. Both proteins are shown here to efficiently extract heme from hemoglobin. Surprisingly, while Pga7 has a higher affinity for heme in vitro, we find that heme transfer can occur bi-directionally between Pga7 and Rbt5, supporting a model in which they cooperate in a heme-acquisition relay. Together, our data delineate the roles of Pga7 and Rbt5 in a cell surface protein network that transfers heme from extracellular hemoglobin to the endocytic pathway, and provide a paradigm for how receptors embedded in the cell wall matrix can mediate nutrient uptake across the fungal cell envelope. PMID:25275454

Iron acquisition by Haemophilus influenzae.

PubMed Central

Pidcock, K A; Wooten, J A; Daley, B A; Stull, T L

1988-01-01

The mechanisms for acquisition of iron by Haemophilus influenzae and their role in pathogenesis are not known. Heme and nonheme sources of iron were evaluated for their effect on growth of type b and nontypable strains of H. influenzae in an iron-restricted, defined medium. All 13 strains acquired iron from heme, hemoglobin, hemoglobin-haptoglobin, and heme-hemopexin. Among nonheme sources of protein-bound iron, growth of H. influenzae was enhanced by partially saturated human transferrin but not by lactoferrin or ferritin. Purified ferrienterochelin and ferridesferrioxamine failed to provide iron to H. influenzae, and the supernatants of H. influenzae E1a grown in iron-restricted medium failed to enhance iron-restricted growth of siderophore-dependent strains of Escherichia coli, Salmonella typhimurium, and Arthrobacter terregens. Marked alterations in the profile of outer membrane proteins of H. influenzae were observed when the level of free iron was varied between 1 microM and 1 mM. Catechols were not detected in the supernatants of strain E1a; however, iron-related hydroxamate production was detected by two biochemical assays. We conclude that the sources of iron for H. influenzae are diverse. The significance of hydroxamate production and iron-related outer membrane proteins to H. influenzae iron acquisition is not yet clear. Images PMID:2964410

Acquisition of complement inhibitor serine protease factor I and its cofactors C4b-binding protein and factor H by Prevotella intermedia.

PubMed

Malm, Sven; Jusko, Monika; Eick, Sigrun; Potempa, Jan; Riesbeck, Kristian; Blom, Anna M

2012-01-01

Infection with the Gram-negative pathogen Prevotella intermedia gives rise to periodontitis and a growing number of studies implies an association of P. intermedia with rheumatoid arthritis. The serine protease Factor I (FI) is the central inhibitor of complement degrading complement components C3b and C4b in the presence of cofactors such as C4b-binding protein (C4BP) and Factor H (FH). Yet, the significance of complement inhibitor acquisition in P. intermedia infection and FI binding by Gram-negative pathogens has not been addressed. Here we show that P. intermedia isolates bound purified FI as well as FI directly from heat-inactivated human serum. FI bound to bacteria retained its serine protease activity as shown in degradation experiments with (125)I-labeled C4b. Since FI requires cofactors for its activity we also investigated the binding of purified cofactors C4BP and FH and found acquisition of both proteins, which retained their activity in FI mediated degradation of C3b and C4b. We propose that FI binding by P. intermedia represents a new mechanism contributing to complement evasion by a Gram-negative bacterial pathogen associated with chronic diseases.

Protein kinase D1 is essential for bone acquisition during pubertal growth.

PubMed

Ford, Jeffery J; Yeh, Lee-Chuan C; Schmidgal, Eric C; Thompson, Jason F; Adamo, Martin L; Lee, John C

2013-11-01

Bone formation and maintenance represents the summation of the balance of local and endocrine hormonal stimuli within a complex organ. Protein kinase D (PKD) is a member of the Ca(2+)/calmodulin-dependent kinase superfamily of serine/threonine kinases and has been described as the crossroads for the bone morphogenetic protein (BMP)-IGF-I signaling axis, which plays a major role in bone formation. The current study exploits the PKD1-deficient mouse model to examine the role of PKD in vivo in the skeleton. Dual-energy x-ray absorptiometry scan analysis of male and female pubescent mice demonstrated significantly decreased bone mineral density in the whole body and femoral bone compartments of PKD1 (+/-) mice, compared with their wild-type littermates. The body weight, nasal-anal length, and percentage body fat of the mice were not significantly different from their wild-type littermates. Cultured bone marrow stromal cells from PKD1 (+/-) mice demonstrated lower alkaline phosphatase activity in early differentiating osteoblasts and decreased mineralized nodule formation in mature osteoblasts. Quantitative RT-PCR analysis of osteoblast differentiation markers and osteoclast markers exhibited lower levels of expression in PKD1 (+/-) male mice than wild type. In female mice, however, only markers of osteoblast differentiation were reduced. PKD1 (+/-) mice also demonstrated a profound reduction in mRNA expression levels of BMP type II receptor and IGF-I receptor and in BMP-7 responsiveness in vitro. Together these data suggest that in mice, PKD1 action contributes to the regulation of osteoblastogenesis by altering gene expression with gender-specific effects on osteoclastogenesis, subsequently affecting skeletal matrix acquisition during puberty.

Acquisition of Complement Inhibitor Serine Protease Factor I and Its Cofactors C4b-Binding Protein and Factor H by Prevotella intermedia

PubMed Central

Malm, Sven; Jusko, Monika; Eick, Sigrun; Potempa, Jan; Riesbeck, Kristian; Blom, Anna M.

2012-01-01

Infection with the Gram-negative pathogen Prevotella intermedia gives rise to periodontitis and a growing number of studies implies an association of P. intermedia with rheumatoid arthritis. The serine protease Factor I (FI) is the central inhibitor of complement degrading complement components C3b and C4b in the presence of cofactors such as C4b-binding protein (C4BP) and Factor H (FH). Yet, the significance of complement inhibitor acquisition in P. intermedia infection and FI binding by Gram-negative pathogens has not been addressed. Here we show that P. intermedia isolates bound purified FI as well as FI directly from heat-inactivated human serum. FI bound to bacteria retained its serine protease activity as shown in degradation experiments with 125I-labeled C4b. Since FI requires cofactors for its activity we also investigated the binding of purified cofactors C4BP and FH and found acquisition of both proteins, which retained their activity in FI mediated degradation of C3b and C4b. We propose that FI binding by P. intermedia represents a new mechanism contributing to complement evasion by a Gram-negative bacterial pathogen associated with chronic diseases. PMID:22514678

Biological effects of low energy nitrogen ion implantation on Jatropha curcas L. seed germination

NASA Astrophysics Data System (ADS)

Xu, Gang; Wang, Xiao-teng; Gan, Cai-ling; Fang, Yan-qiong; Zhang, Meng

2012-09-01

To explore the biological effects of nitrogen ion beam implantation on dry Jatropha curcas seed, a beam of N+ with energy of 25 keV was applied to treat the dry seed at six different doses. N+ beam implantation greatly decreased germination rate and seedling survival rate. The doses within the range of 12 × 1016 to 15 × 1016 ions cm-2 severely damaged the seeds: total antioxidant capacity (TAC), germination rate, seedling survival rate, reduced ascorbate acid (HAsA) and reduced glutathione (GSH) contents, and most of the tested antioxidases activity (i.e. catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase (SOD)) reached their lowest levels. At a dose of 18 × 1016 ion cm-2, biological repair took place: moderate increases were found in TAC, germination rate, seedling survival rate, HAsA and GSH contents, and some antioxidant enzyme activities (i.e. CAT, APX, SOD and GPX). The dose of 18 × 1016 ions cm-2 may be the optimum dose for use in dry J. curcas seed mutation breeding. CAT, HAsA and GSH contributed to the increase of TAC, but CAT was the most important. POD performed its important role as seed was severely damaged. The main role of the HAsA-GSH cycle appeared to be for regeneration of HAsA.

Interobserver repeatability of measurements on computed tomography images of lax canine hip joints from youth to maturity.

PubMed

Lopez, Mandi J; Davis, Kechia M; Jeffrey-Borger, Susan L; Markel, Mark D; Rettenmund, Christy

2009-12-01

To determine interobserver repeatability of measurements on computed tomography (CT) images of lax canine hip joints at different ages and in the presence of degenerative joint disease at maturity. Longitudinal observational investigation. Sibling crossbreed hounds. Pelvic CT was performed at 20, 24, 32, 48, 68, and 104 weeks of age. Measures were performed on 3 contiguous two-dimensional (2D) transverse CT images of both hips at each time point by 3 investigators. Center-edge angle (CEA), horizontal toit externe angle (HTEA), ventral (VASA), dorsal (DASA), and horizontal (HASA) acetabular sector angles, acetabular index (AI), and percent femoral head coverage (CPC) were measured. Interobserver repeatability was quantified with the intraclass correlation coefficient (ICC). Satisfactory repeatability was considered when ICC >or=0.75. DASA, CEA, and CPC were repeatable in all age groups. HASA and HTEA were repeatable for all but 1 time point. At 20 weeks of age, all measures but AI were repeatable, and at 104 weeks of age, DASA, CEA, CPC, and HASA were repeatable. Measures were repeatable in hips with and without degenerative changes with the exceptions of AI and HASA in normal hips and VASA and HTEA in osteoarthritic hips. Most 2D CT measurements examined were repeatable regardless of age or joint disease. Two-dimensional CT measures may augment current techniques for assessing joint changes in lax canine hips.

Protein Contribution to Plant Salinity Response and Tolerance Acquisition

PubMed Central

Kosová, Klára; Prášil, Ilja T.; Vítámvás, Pavel

2013-01-01

The review is focused on plant proteome response to salinity with respect to physiological aspects of plant salt stress response. The attention is paid to both osmotic and ionic effects of salinity stress on plants with respect to several protein functional groups. Therefore, the role of individual proteins involved in signalling, changes in gene expression, protein biosynthesis and degradation and the resulting changes in protein relative abundance in proteins involved in energy metabolism, redox metabolism, stressand defence-related proteins, osmolyte metabolism, phytohormone, lipid and secondary metabolism, mechanical stress-related proteins as well as protein posttranslational modifications are discussed. Differences between salt-sensitive (glycophytes) and salt-tolerant (halophytes) plants are analysed with respect to differential salinity tolerance. In conclusion, contribution of proteomic studies to understanding plant salinity tolerance is summarised and discussed. PMID:23531537

Ratiometric Raman Spectroscopy for Quantification of Protein Oxidative Damage

PubMed Central

Jiang, Dongping; Yanney, Michael; Zou, Sige; Sygula, Andrzej

2009-01-01

A novel ratiometric Raman spectroscopic (RMRS) method has been developed for quantitative determination of protein carbonyl levels. Oxidized bovine serum albumin (BSA) and oxidized lysozyme were used as model proteins to demonstrate this method. The technique involves conjugation of protein carbonyls with dinitrophenyl hydrazine (DNPH), followed by drop coating deposition Raman spectral acquisition (DCDR). The RMRS method is easy to implement as it requires only one conjugation reaction, a single spectral acquisition, and does not require sample calibration. Characteristic peaks from both protein and DNPH moieties are obtained in a single spectral acquisition, allowing the protein carbonyl level to be calculated from the peak intensity ratio. Detection sensitivity for the RMRS method is ~0.33 pmol carbonyl/measurement. Fluorescence and/or immunoassay based techniques only detect a signal from the labeling molecule and thus yield no structural or quantitative information for the modified protein while the RMRS technique provides for protein identification and protein carbonyl quantification in a single experiment. PMID:19457432

Protein synthesis during acquisition of long-term facilitation is needed for the persistent loss of regulatory subunits of the Aplysia cAMP-dependent protein kinase.

PubMed Central

Bergold, P J; Sweatt, J D; Winicov, I; Weiss, K R; Kandel, E R; Schwartz, J H

1990-01-01

Depending on the number or the length of exposure, application of serotonin can produce either short-term or long-term presynaptic facilitation of Aplysia sensory-to-motor synapses. The cAMP-dependent protein kinase, a heterodimer of two regulatory and two catalytic subunits, has been shown to become stably activated only during long-term facilitation. Both acquisition of long-term facilitation and persistent activation of the kinase is blocked by anisomycin, an effective, reversible, and specific inhibitor of protein synthesis in Aplysia. We report here that 2-hr exposure of pleural sensory cells to serotonin lowers the concentration of regulatory subunits but does not change the concentration of catalytic subunits, as assayed 24 hr later; 5-min exposure to serotonin has no effect on either type of subunit. Increasing intracellular cAMP with a permeable analog of cAMP together with the phosphodiesterase inhibitor isobutyl methylxanthine also decreased regulatory subunits, suggesting that cAMP is the second messenger mediating serotonin action. Anisomycin blocked the loss of regulatory subunits only when applied with serotonin; application after the 2-hr treatment with serotonin had no effect. In the Aplysia accessory radula contractor muscle, prolonged exposure to serotonin or to the peptide transmitter small cardioactive peptide B, both of which produce large increases in intracellular cAMP, does not decrease regulatory subunits. This mechanism of regulating the cAMP-dependent protein kinase therefore may be specific to the nervous system. We conclude that during long-term facilitation, new protein is synthesized in response to the facilitatory stimulus, which changes the ratio of subunits of the cAMP-dependent protein kinase. This alteration in ratio could persistently activate the kinase and produce the persistent phosphorylation seen in long-term facilitated sensory cells. Images PMID:1692622

Knowledge, attitude and practice of ministry of health primary health care physicians in the management of type 2 diabetes mellitus: a cross-sectional study in the Al Hasa District of Saudi Arabia, 2010.

PubMed

Khan, A R; Al Abdul Lateef, Z Nase; Khamseen, Ma B; Al Aithan, M A; Khan, S A; Al Ibrahim, I

2011-01-01

To assess the Knowledge Attitude and Practice (KAP) of MOH Primary Health Care Physician in the management of Type 2 Diabetes Mellitus (DM). A cross sectional survey was conduced between April and October 2010 on MOH Primary Health Care physicians working in Al Hasa district of Saudi Arabia by filling up of pre-tested specially designed questionnaires focused on Knowledge, Attitude and practice towards Type 2 DM patients. The answers were scored by assigning marks. A SPSS 18 was used for statistical analysis. The mean of overall KAP score (± SD) for all the respondents were 66.59 ± 8.82 (Maximum 100). Male physicians scored better than the females physicians (66.90, P=.018 Vs 64.67, P=.018) and the same was true with the rural physicians who scored higher (68.65 ± 10.19, P=.003) than the urban physicians (65.34 ± 7.36, P=.003).The main weakness of knowledge was on epidemiology of Diabetes Mellitus (DM). 28.3 % (n=28) of physicians didn't know the correct diagnostic criteria of Type 2 DM and only 34.7% physicians knew the correct angle of insulin injection. 86.8% (n=86) of the physicians did not agree that Diabetic Self Management Education (DSME) is an essential part of diabetic care. The mean overall KAP score was significantly higher for physicians with 1-5 years of experience (70.16, P=<.05)) than those with more than 5 years of practice (P=<.05). This difference was found in all the segments of KAP. Overall KAP score of those GPs who had a copy of Clinical practice guidelines (CPG) in their clinic was 70.90 ± 10.94 which was higher than KAP Score of those GPs who did not have a copy of CPG (65.10 ± 7.01, P=.005). Checking patients' ability to manage their diabetes, checking blood pressure, eye exam, lipids , serum creatinine, baseline ECG, chest X ray and serum electrolyte were the best followed tests while foot exam and urinary protein were performed more often than recommended in the guideline. Serum HbAc1 was the most delayed test. This study explored

Application of wide selected-ion monitoring data-independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor.

PubMed

Martin, Laetitia B B; Sherwood, Robert W; Nicklay, Joshua J; Yang, Yong; Muratore-Schroeder, Tara L; Anderson, Elizabeth T; Thannhauser, Theodore W; Rose, Jocelyn K C; Zhang, Sheng

2016-08-01

We describe here the use of label-free wide selected-ion monitoring data-independent acquisition (WiSIM-DIA) to identify proteins that are involved in the formation of tomato (Solanum lycopersicum) fruit cuticles and that are regulated by the transcription factor CUTIN DEFICIENT2 (CD2). A spectral library consisting of 11 753 unique peptides, corresponding to 2338 tomato protein groups, was used and the DIA analysis was performed at the MS1 level utilizing narrow mass windows for extraction with Skyline 2.6 software. We identified a total of 1140 proteins, 67 of which had expression levels that differed significantly between the cd2 tomato mutant and the wild-type cultivar M82. Differentially expressed proteins including a key protein involved in cutin biosynthesis, were selected for validation by target SRM/MRM and by Western blot analysis. In addition to confirming a role for CD2 in regulating cuticle formation, the results also revealed that CD2 influences pathways associated with cell wall biology, anthocyanin biosynthesis, plant development, and responses to stress, which complements findings of earlier RNA-Seq experiments. Our results provide new insights into molecular processes and aspects of fruit biology associated with CD2 function, and demonstrate that the WiSIM-DIA is an effective quantitative approach for global protein identifications. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Application of wide selected-ion monitoring data-independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor

PubMed Central

Martin, Laetitia B. B.; Sherwood, Robert W.; Nicklay, Joshua J.; Yang, Yong; Muratore-Schroeder, Tara L.; Anderson, Elizabeth T.; Thannhauser, Theodore W.; Rose, Jocelyn K. C.; Zhang, Sheng

2017-01-01

We describe here the use of label-free wide selected-ion monitoring data-independent acquisition (WiSIM-DIA) to identify proteins that are involved in the formation of tomato (Solanum lycopersicum) fruit cuticles and that are regulated by the transcription factor CUTIN DEFICIENT2 (CD2). A spectral library consisting of 11 753 unique peptides, corresponding to 2338 tomato protein groups, was used and the DIA analysis was performed at the MS1 level utilizing narrow mass windows for extraction with Skyline 2.6 software. We identified a total of 1140 proteins, 67 of which had expression levels that differed significantly between the cd2 tomato mutant and the wild-type cultivar M82. Differentially expressed proteins including a key protein involved in cutin biosynthesis, were selected for validation by target SRM/MRM and by Western blot analysis. In addition to confirming a role for CD2 in regulating cuticle formation, the results also revealed that CD2 influences pathways associated with cell wall biology, anthocyanin biosynthesis, plant development, and responses to stress, which complements findings of earlier RNA-Seq experiments. Our results provide new insights into molecular processes and aspects of fruit biology associated with CD2 function, and demonstrate that the WiSIM-DIA is an effective quantitative approach for global protein identifications. PMID:27089858

Mapping differential interactomes by affinity purification coupled with data-independent mass spectrometry acquisition.

PubMed

Lambert, Jean-Philippe; Ivosev, Gordana; Couzens, Amber L; Larsen, Brett; Taipale, Mikko; Lin, Zhen-Yuan; Zhong, Quan; Lindquist, Susan; Vidal, Marc; Aebersold, Ruedi; Pawson, Tony; Bonner, Ron; Tate, Stephen; Gingras, Anne-Claude

2013-12-01

Characterizing changes in protein-protein interactions associated with sequence variants (e.g., disease-associated mutations or splice forms) or following exposure to drugs, growth factors or hormones is critical to understanding how protein complexes are built, localized and regulated. Affinity purification (AP) coupled with mass spectrometry permits the analysis of protein interactions under near-physiological conditions, yet monitoring interaction changes requires the development of a robust and sensitive quantitative approach, especially for large-scale studies in which cost and time are major considerations. We have coupled AP to data-independent mass spectrometric acquisition (sequential window acquisition of all theoretical spectra, SWATH) and implemented an automated data extraction and statistical analysis pipeline to score modulated interactions. We used AP-SWATH to characterize changes in protein-protein interactions imparted by the HSP90 inhibitor NVP-AUY922 or melanoma-associated mutations in the human kinase CDK4. We show that AP-SWATH is a robust label-free approach to characterize such changes and propose a scalable pipeline for systems biology studies.

Selection of Oil Coolers to Avoid Congealing

DTIC Science & Technology

1943-07-01

from one fluid to another, there is a resistance to heat transfer l/ htSt . The total resistance to the flow of heat is the sum of the...total thermal resistance equation is, htst hasa hoso n v \\hasa Vo/ (W The generally accepted equations for correlating heat...1 -. 1 htSt n v Da jS u Do Ho •a°l|.ki (5i2jLS 8oc3lc0/ W^ V \\SAafa; ^ fa^Z/yfij tM: (7) The heat-balance equation is. H

Absolute quantitation of disease protein biomarkers in a single LC-MS acquisition using apolipoprotein F as an example.

PubMed

Kumar, Abhinav; Gangadharan, Bevin; Cobbold, Jeremy; Thursz, Mark; Zitzmann, Nicole

2017-09-21

LC-MS and immunoassay can detect protein biomarkers. Immunoassays are more commonly used but can potentially be outperformed by LC-MS. These techniques have limitations including the necessity to generate separate calibration curves for each biomarker. We present a rapid mass spectrometry-based assay utilising a universal calibration curve. For the first time we analyse clinical samples using the HeavyPeptide IGNIS kit which establishes a 6-point calibration curve and determines the biomarker concentration in a single LC-MS acquisition. IGNIS was tested using apolipoprotein F (APO-F), a potential biomarker for non-alcoholic fatty liver disease (NAFLD). Human serum and IGNIS prime peptides were digested and the IGNIS assay was used to quantify APO-F in clinical samples. Digestion of IGNIS prime peptides was optimised using trypsin and SMART Digest™. IGNIS was 9 times faster than the conventional LC-MS method for determining the concentration of APO-F in serum. APO-F decreased across NAFLD stages. Inter/intra-day variation and stability post sample preparation for one of the peptides was ≤13% coefficient of variation (CV). SMART Digest™ enabled complete digestion in 30 minutes compared to 24 hours using in-solution trypsin digestion. We have optimised the IGNIS kit to quantify APO-F as a NAFLD biomarker in serum using a single LC-MS acquisition.

AKAPS Act in a Two-Step Mechanism of Memory Acquisition

PubMed Central

Scheunemann, Lisa; Skroblin, Philipp; Hundsrucker, Christian; Klussmann, Enno; Efetova, Marina

2013-01-01

Defining the molecular and neuronal basis of associative memories is based upon behavioral preparations that yield high performance due to selection of salient stimuli, strong reinforcement, and repeated conditioning trials. One of those preparations is the Drosophila aversive olfactory conditioning procedure where animals initiate multiple memory components after experience of a single cycle training procedure. Here, we explored the analysis of acquisition dynamics as a means to define memory components and revealed strong correlations between particular chronologies of shock impact and number experienced during the associative training situation and subsequent performance of conditioned avoidance. Analyzing acquisition dynamics in Drosophila memory mutants revealed that rutabaga (rut)-dependent cAMP signals couple in a divergent fashion for support of different memory components. In case of anesthesia-sensitive memory (ASM) we identified a characteristic two-step mechanism that links rut-AC1 to A-kinase anchoring proteins (AKAP)-sequestered protein kinase A at the level of Kenyon cells, a recognized center of olfactory learning within the fly brain. We propose that integration of rut-derived cAMP signals at level of AKAPs might serve as counting register that accounts for the two-step mechanism of ASM acquisition. PMID:24174675

Expression of activity-regulated cytoskeleton-associated protein (Arc/Arg3.1) in the nucleus accumbens is critical for the acquisition, expression and reinstatement of morphine-induced conditioned place preference.

PubMed

Lv, Xiu-Fang; Xu, Ya; Han, Ji-Sheng; Cui, Cai-Lian

2011-09-30

Activity-regulated cytoskeleton-associated protein (Arc), also known as activity-regulated gene 3.1 (Arg3.1), is an immediate early gene whose mRNA is selectively targeted to recently activated synaptic sites, where it is translated and enriched. This unique feature suggests a role for Arc/Arg3.1 in coupling synaptic activity to protein synthesis, leading to synaptic plasticity. Although the Arc/Arg3.1 gene has been shown to be induced by a variety of abused drugs and its protein has been implicated in diverse forms of long-term memory, relatively little is known about its role in drug-induced reward memory. In this study, we investigated the potential role of Arc/Arg3.1 protein expression in reward-related associative learning and memory using morphine-induced conditioned place preference (CPP) in rats. We found that (1) intraperitoneal (i.p.) injection of morphine (10mg/kg) increased Arc/Arg3.1 protein levels after 2h in the NAc core but not in the NAc shell. (2) In CPP experiments, Arc/Arg3.1 protein was increased in the NAc shell of rats following both morphine conditioning and the CPP expression test compared to rats that received the conditioning without the test or those that did not receive morphine conditioning. (3) Microinjection of Arc/Arg3.1 antisense oligodeoxynucleotide (AS) into the NAc core inhibited the acquisition, expression and reinstatement of morphine CPP; however, intra-NAc shell infusions of the AS only blocked the expression of CPP. These findings suggest that expression of the Arc/Arg3.1 protein in the NAc core is required for the acquisition, context-induced retrieval and reinstatement of morphine-associated reward memory, whereas Arc/Arg3.1 protein expression in the NAc shell is only critical for the context-induced retrieval of memory. As a result, Arc/Arg3.1 may be a potential therapeutic target for the prevention of drug abuse or the relapse of drug use. Copyright © 2011 Elsevier B.V. All rights reserved.

Regulatory protein BBD18 of the lyme disease spirochete: essential role during tick acquisition?

PubMed

Hayes, Beth M; Dulebohn, Daniel P; Sarkar, Amit; Tilly, Kit; Bestor, Aaron; Ambroggio, Xavier; Rosa, Patricia A

2014-04-01

The Lyme disease spirochete Borrelia burgdorferi senses and responds to environmental cues as it transits between the tick vector and vertebrate host. Failure to properly adapt can block transmission of the spirochete and persistence in either vector or host. We previously identified BBD18, a novel plasmid-encoded protein of B. burgdorferi, as a putative repressor of the host-essential factor OspC. In this study, we investigate the in vivo role of BBD18 as a regulatory protein, using an experimental mouse-tick model system that closely resembles the natural infectious cycle of B. burgdorferi. We show that spirochetes that have been engineered to constitutively produce BBD18 can colonize and persist in ticks but do not infect mice when introduced by either tick bite or needle inoculation. Conversely, spirochetes lacking BBD18 can persistently infect mice but are not acquired by feeding ticks. Through site-directed mutagenesis, we have demonstrated that abrogation of spirochete infection in mice by overexpression of BBD18 occurs only with bbd18 alleles that can suppress OspC synthesis. Finally, we demonstrate that BBD18-mediated regulation does not utilize a previously described ospC operator sequence required by B. burgdorferi for persistence in immunocompetent mice. These data lead us to conclude that BBD18 does not represent the putative repressor utilized by B. burgdorferi for the specific downregulation of OspC in the mammalian host. Rather, we suggest that BBD18 exhibits features more consistent with those of a global regulatory protein whose critical role occurs during spirochete acquisition by feeding ticks. IMPORTANCE Lyme disease, caused by Borrelia burgdorferi, is the most common arthropod-borne disease in North America. B. burgdorferi is transmitted to humans and other vertebrate hosts by ticks as they take a blood meal. Transmission between vectors and hosts requires the bacterium to sense changes in the environment and adapt. However, the mechanisms

76 FR 44280 - Defense Federal Acquisition Regulation Supplement; Simplified Acquisition Threshold for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-25

...-AH29 Defense Federal Acquisition Regulation Supplement; Simplified Acquisition Threshold for.... 2302(7) to invoke a simplified acquisition threshold that is two times the amount specified at 41 U.S.C... acquisition threshold is $150,000 as specified in Federal Acquisition Regulation 2.101. DATES: Effective Date...

Language Acquisition without an Acquisition Device

ERIC Educational Resources Information Center

O'Grady, William

2012-01-01

Most explanatory work on first and second language learning assumes the primacy of the acquisition phenomenon itself, and a good deal of work has been devoted to the search for an "acquisition device" that is specific to humans, and perhaps even to language. I will consider the possibility that this strategy is misguided and that language…

Late Protein Synthesis-Dependent Phases in CTA Long-Term Memory: BDNF Requirement

PubMed Central

Martínez-Moreno, Araceli; Rodríguez-Durán, Luis F.; Escobar, Martha L.

2011-01-01

It has been proposed that long-term memory (LTM) persistence requires a late protein synthesis-dependent phase, even many hours after memory acquisition. Brain-derived neurotrophic factor (BDNF) is an essential protein synthesis product that has emerged as one of the most potent molecular mediators for long-term synaptic plasticity. Studies in the rat hippocampus have been shown that BDNF is capable to rescue the late-phase of long-term potentiation as well as the hippocampus-related LTM when protein synthesis was inhibited. Our previous studies on the insular cortex (IC), a region of the temporal cortex implicated in the acquisition and storage of conditioned taste aversion (CTA), have demonstrated that intracortical delivery of BDNF reverses the deficit in CTA memory caused by the inhibition of IC protein synthesis due to anisomycin administration during early acquisition. In this work, we first analyze whether CTA memory storage is protein synthesis-dependent in different time windows. We observed that CTA memory become sensible to protein synthesis inhibition 5 and 7 h after acquisition. Then, we explore the effect of BDNF delivery (2 μg/2 μl per side) in the IC during those late protein synthesis-dependent phases. Our results show that BDNF reverses the CTA memory deficit produced by protein synthesis inhibition in both phases. These findings support the notion that recurrent rounds of consolidation-like events take place in the neocortex for maintenance of CTA memory trace and that BDNF is an essential component of these processes. PMID:21960964

75 FR 51416 - Defense Federal Acquisition Regulation Supplement; Acquisition of Commercial Items

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-20

... be used for the acquisition of commercial items, and revised the language to address the conditions... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Parts 202, 212, and 234 [DFARS Case 2008-D011] Defense Federal Acquisition Regulation Supplement; Acquisition of Commercial Items...

76 FR 71833 - Defense Federal Acquisition Regulation Supplement: Simplified Acquisition Threshold for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-18

...-AH29 Defense Federal Acquisition Regulation Supplement: Simplified Acquisition Threshold for... statutory authority to invoke a simplified acquisition threshold that is two times the normal amount to...) to invoke a simplified acquisition threshold that is two times the amount specified at 41 U.S.C 134...

An Acquisition Guide for Executives

EPA Pesticide Factsheets

This guide covers the following subjects; What is Acquisition?, Purpose and Primary Functions of the Agency’s Acquisition System, Key Organizations in Acquisitions, Legal Framework, Key Players in Acquisitions, Acquisition Process, Acquisition Thresholds

33. Perimeter acquisition radar building room #320, perimeter acquisition radar ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. Perimeter acquisition radar building room #320, perimeter acquisition radar operations center (PAROC), contains the tactical command and control group equipment required to control the par site. Showing spacetrack monitor console - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

Microcomputer-Based Acquisitions.

ERIC Educational Resources Information Center

Desmarais, Norman

1986-01-01

This discussion of three automated acquisitions systems--Bib-Base/Acq, The Book Trak Ordering System, and Card Datalog Acquisitions Module--covers searching and updating, editing, acquisitions functions and statistics, purchase orders and order file, budgeting and accounts maintenance, defining parameters, documentation, security, printing, and…

Multitrophic Cry-protein flow in Bt-cotton

USDA-ARS?s Scientific Manuscript database

Although most genetically engineered cotton plants grown today produce the insecticidal Cry-proteins Cry1Ac and Cry2Ab, studies are lacking on multitrophic Cry-protein acquisition in dual-gene cotton fields. Such field data are important for the design and interpretation of laboratory risk assessmen...

Data-Independent Acquisition-Based Quantitative Proteomic Analysis Reveals Potential Biomarkers of Kidney Cancer.

PubMed

Song, Yimeng; Zhong, Lijun; Zhou, Juntuo; Lu, Min; Xing, Tianying; Ma, Lulin; Shen, Jing

2017-12-01

Renal cell carcinoma (RCC) is a malignant and metastatic cancer with 95% mortality, and clear cell RCC (ccRCC) is the most observed among the five major subtypes of RCC. Specific biomarkers that can distinguish cancer tissues from adjacent normal tissues should be developed to diagnose this disease in early stages and conduct a reliable prognostic evaluation. Data-independent acquisition (DIA) strategy has been widely employed in proteomic analysis because of various advantages, including enhanced protein coverage and reliable data acquisition. In this study, a DIA workflow is constructed on a quadrupole-Orbitrap LC-MS platform to reveal dysregulated proteins between ccRCC and adjacent normal tissues. More than 4000 proteins are identified, 436 of these proteins are dysregulated in ccRCC tissues. Bioinformatic analysis reveals that multiple pathways and Gene Ontology items are strongly associated with ccRCC. The expression levels of L-lactate dehydrogenase A chain, annexin A4, nicotinamide N-methyltransferase, and perilipin-2 examined through RT-qPCR, Western blot, and immunohistochemistry confirm the validity of the proteomic analysis results. The proposed DIA workflow yields optimum time efficiency and data reliability and provides a good choice for proteomic analysis in biological and clinical studies, and these dysregulated proteins might be potential biomarkers for ccRCC diagnosis. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

77 FR 10665 - General Services Administration Acquisition Regulation; Acquisition-Related Thresholds

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-23

... GENERAL SERVICES ADMINISTRATION 48 CFR Parts 519 and 552 [GSAR Amendment 2012-02; GSAR Case 2011-G502; (Change 54) Docket 2012- 0003, Sequence 1] RIN 3090-AJ24 General Services Administration Acquisition Regulation; Acquisition-Related Thresholds AGENCIES: Office of Acquisition Policy, General...

Investigating Second Language Acquisition.

ERIC Educational Resources Information Center

Jordens, Peter, Ed.; Lalleman, Josine, Ed.

Essays in second language acquisition include: "The State of the Art in Second Language Acquisition Research" (Josine Lalleman); "Crosslinguistic Influence with Special Reference to the Acquisition of Grammar" (Michael Sharwood Smith); "Second Language Acquisition by Adult Immigrants: A Multiple Case Study of Turkish and…

75 FR 5241 - General Services Administration Acquisition Regulation; Rewrite of Part 512, Acquisition of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-02

... Acquisition Regulation; Rewrite of Part 512, Acquisition of Commercial Items AGENCIES: Office of Acquisition... Administration (GSA) is amending the General Services Administration Acquisition Regulation (GSAR) to update the text addressing the acquisition of commercial items. This rule is a result of the GSAM Rewrite...

Acetabular overcoverage in the horizontal plane: an underdiagnosed trigger of early hip arthritis. A CT scan study in young adults.

PubMed

Valera, Màrius; Ibáñez, Natalia; Sancho, Rogelio; Llauger, Jaume; Gich, Ignasi

2018-01-01

Acetabular overcoverage promotes hip osteoarthritis causing a pincer-type femoroacetabular impingement. Acetabular coverage in the horizontal plane is usually poorly defined in imaging studies and may be misdiagnosed. The goal of this study was to analyze the role of acetabular overcoverage measured in the frontal plane and in the horizontal plane by CT scan and to determine its relationship with other anatomic features in the onset of hip arthritis in young adults. We compared prospectively CT scans from two groups of adults of 55 years or younger: the patient group (n = 30) consisted of subjects with diagnosis of early hip arthritis (Tönnis Grade I or II) and the control group (n = 31) consisted of subjects with healthy hips. Two independent observers analyzed centre edge angle (CEA), acetabular anteversion angle (AAA), anterior sector acetabular angle (AASA), posterior sector acetabular angle (PASA), horizontal acetabular sector angle (HASA), femoral anteversion angle (FAVA), alpha angle (AA), and Mckibbin Instability Index (MI). Angles measuring the acetabular coverage on the horizontal plane (AASA, PASA and, HASA) were significantly higher in the patient group (p < 0.001, p = 0.03 and p < 0.001, respectively). Pearson's correlation coefficient showed a positive correlation between CEA and HASA in patients (r = 0.628) and in controls (r = 0.660). However, a high CEA (> 35º) was strongly associated with a high HASA (> 160º) in patients (p = 0.024) but not in controls (p = 0.21), suggesting that pincer should be simultaneously present in the horizontal and frontal plane to trigger hip degeneration. No significant association was detected between a high alpha angle (> 60º) and a high CEA (> 35º suggesting that a mixed pincer-cam aetiology was not prevalent in our series. Multivariate regression analysis showed the most significant predictors of degenerative joint disease were HASA (p = 0.008), AA (p = 0.048) and ASAA (p = 0

Iron acquisition in the cystic fibrosis lung and potential for novel therapeutic strategies

PubMed Central

Tyrrell, Jean

2016-01-01

Iron acquisition is vital to microbial survival and is implicated in the virulence of many of the pathogens that reside in the cystic fibrosis (CF) lung. The multifaceted nature of iron acquisition by both bacterial and fungal pathogens encompasses a range of conserved and species-specific mechanisms, including secretion of iron-binding siderophores, utilization of siderophores from other species, release of iron from host iron-binding proteins and haemoproteins, and ferrous iron uptake. Pathogens adapt and deploy specific systems depending on iron availability, bioavailability of the iron pool, stage of infection and presence of competing pathogens. Understanding the dynamics of pathogen iron acquisition has the potential to unveil new avenues for therapeutic intervention to treat both acute and chronic CF infections. Here, we examine the range of strategies utilized by the primary CF pathogens to acquire iron and discuss the different approaches to targeting iron acquisition systems as an antimicrobial strategy. PMID:26643057

77 FR 69720 - Federal Acquisition Regulation; Interagency Acquisitions: Compliance by Nondefense Agencies With...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-20

... 9000-AM36 Federal Acquisition Regulation; Interagency Acquisitions: Compliance by Nondefense Agencies...D, GSA, and NASA are issuing an interim rule amending the Federal Acquisition Regulation (FAR) to add new requirements specific to the acquisition of supplies and services by nondefense agencies on...

Automated Validation of Results and Removal of Fragment Ion Interferences in Targeted Analysis of Data-independent Acquisition Mass Spectrometry (MS) using SWATHProphet*

PubMed Central

Keller, Andrew; Bader, Samuel L.; Shteynberg, David; Hood, Leroy; Moritz, Robert L.

2015-01-01

Proteomics by mass spectrometry technology is widely used for identifying and quantifying peptides and proteins. The breadth and sensitivity of peptide detection have been advanced by the advent of data-independent acquisition mass spectrometry. Analysis of such data, however, is challenging due to the complexity of fragment ion spectra that have contributions from multiple co-eluting precursor ions. We present SWATHProphet software that identifies and quantifies peptide fragment ion traces in data-independent acquisition data, provides accurate probabilities to ensure results are correct, and automatically detects and removes contributions to quantitation originating from interfering precursor ions. Integration in the widely used open source Trans-Proteomic Pipeline facilitates subsequent analyses such as combining results of multiple data sets together for improved discrimination using iProphet and inferring sample proteins using ProteinProphet. This novel development should greatly help make data-independent acquisition mass spectrometry accessible to large numbers of users. PMID:25713123

77 FR 56737 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-61; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-13

... Administration National Aeronautics and Space Administration 48 CFR Chapter 1 Federal Acquisition Regulations... AND SPACE ADMINISTRATION 48 CFR Chapter 1 [Docket FAR 2012-0080, Sequence 6] Federal Acquisition Regulation; Federal Acquisition Circular 2005-61; Introduction AGENCY: Department of Defense (DoD), General...

76 FR 39231 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-53; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-05

... National Aeronautics and Space Administration 48 CFR Chapter 1 Federal Acquisition Regulation; Final Rules... ADMINISTRATION 48 CFR Chapter 1 [Docket FAR 2011-0076, Sequence 5] Federal Acquisition Regulation; Federal Acquisition Circular 2005-53; Introduction AGENCIES: Department of Defense (DoD), General Services...

77 FR 2653 - Defense Federal Acquisition Regulation Supplement; Pilot Program for Acquisition of Military...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-19

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Parts 212 and 252 RIN 0750-AH27 Defense Federal Acquisition Regulation Supplement; Pilot Program for Acquisition of Military-Purpose Nondevelopmental Items (DFARS Case 2011-D034) AGENCY: Defense Acquisition Regulations System...

78 FR 60167 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-70; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-30

...)(3), contracting officers are encouraged to include the changes in these rules in major modifications.... William Clark, Acting Director, Office of Government-wide Acquisition Policy, Office of Acquisition Policy, Office of Government-wide Policy. Federal Acquisition Circular (FAC) 2005-70 is issued under the...

The knowledge attitude and practice regarding diabetes and diabetic retinopathy among the final year medical students of King Faisal University Medical College of Al Hasa region of Saudi Arabia: a cross sectional survey.

PubMed

Al Wadaani, F A

2013-01-01

The purpose of the study was to assess the knowledge attitude and practice of the final year medical students of king faisal university medical college towards diabetes and diabetic retinopathy. A cross sectional survey was conducted in King Faisal university Medical college of Al Hasa province of Saudi Arabia in May 2012. The student population was the Final Year students of MBBS. All 96 students of final year MBBS were included in this study. The data were collected by means of filling up of pre-tested specially designed questionnaires focused on Knowledge, Attitude and practice towards Diabetes Mellitus and Diabetic retinopathy. The answers were scored by assigning marks. A SPSS 17 was used for statistical analysis. The mean of overall KAP score (±SD) for all the respondents were 64.75 ± 11.17 (Maximum 100). Male students scored better than the females students (65.30, P = 0.02 vs. 64.18, P = 0.02). The male student scored better in the knowledge (35.83 vs. 34.05, P = 0.018) and practice (13.63 vs. 12.95, P = 0.045) category while the attitude score of female student (17.16 vs. 15.83, P = 0 .020) was significantly higher than the male students. The main weakness of knowledge was on epidemiology of Diabetes Mellitus (DM), the follow up of the diabetic patient for the screening of diabetic retinopathy and the relation of duration of diabetes with the development and progression of diabetic retinopathy. Many students believed that diabetes is more prevalent among the uneducated people. Ninety percent students did not know the proper angle of insulin injection. This survey highlights some of the lacuna in the teaching system of the medical students and identified the need for improvement in their knowledge, attitude and practices for treating the patients with diabetes and diabetic retinopathy. This can be done by incorporating special syllabus focusing on diabetes and diabetic retinopathy in their teaching programme.

Identification of the heme acquisition system in Vibrio vulnificus M2799.

PubMed

Kawano, Hiroaki; Miyamoto, Katsushiro; Yasunobe, Megumi; Murata, Masahiro; Yamahata, Eri; Yamaguchi, Ryo; Miyaki, Yuta; Tsuchiya, Takahiro; Tanabe, Tomotaka; Funahashi, Tatsuya; Tsujibo, Hiroshi

2018-04-01

Vibrio vulnificus, the causative agent of serious, often fatal, infections in humans, requires iron for its pathogenesis. As such, it obtains iron via both vulnibactin and heme-mediated iron-uptake systems. In this study, we identified the heme acquisition system in V. vulnificus M2799. The nucleotide sequences of the genes encoding heme receptors HupA and HvtA and the ATP-binding cassette (ABC) transport system proteins HupB, HupC, and HupD were determined, and then used in the construction of deletion mutants developed from a Δics strain, which could not synthesize vulnibactin. Growth experiments using these mutants indicated that HupA and HvtA are major and minor heme receptors, respectively. The expressions of two proteins were analyzed by the quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Furthermore, complementation analyses confirmed that the HupBCD proteins are the only ABC transport system shared by both the HupA and HvtA receptors. This is the first genetic evidence that the HupBCD proteins are essential for heme acquisition by V. vulnificus. Further investigation showed that hupA, hvtA, and hupBCD are regulated by Fur. The qRT-PCR analysis of the heme receptor genes revealed that HupR, a LysR-family positive transcriptional activator, upregulates the expression of hupA, but not hvtA. In addition, ptrB was co-transcribed with hvtA, and PtrB had no influence on growth in low-iron CM9 medium supplemented with hemin, hemoglobin, or cytochrome C. Copyright © 2018 Elsevier Ltd. All rights reserved.

75 FR 39413 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-44; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-08

... Acquisition Council and the Defense Acquisition Regulations Council in this Federal Acquisition Circular (FAC) 2005-44. A companion document, the Small Entity Compliance Guide (SECG), follows this FAC. The FAC.... Please cite FAC 2005-44 and the FAR case number. Interested parties may also visit our Web site at http...

Iron Acquisition in Bacillus cereus: The Roles of IlsA and Bacillibactin in Exogenous Ferritin Iron Mobilization

PubMed Central

Buisson, Christophe; Daou, Nadine; Kallassy, Mireille; Lereclus, Didier; Arosio, Paolo; Bou-Abdallah, Fadi; Nielsen Le Roux, Christina

2014-01-01

In host-pathogen interactions, the struggle for iron may have major consequences on the outcome of the disease. To overcome the low solubility and bio-availability of iron, bacteria have evolved multiple systems to acquire iron from various sources such as heme, hemoglobin and ferritin. The molecular basis of iron acquisition from heme and hemoglobin have been extensively studied; however, very little is known about iron acquisition from host ferritin, a 24-mer nanocage protein able to store thousands of iron atoms within its cavity. In the human opportunistic pathogen Bacillus cereus, a surface protein named IlsA (Iron-regulated leucine rich surface protein type A) binds heme, hemoglobin and ferritin in vitro and is involved in virulence. Here, we demonstrate that IlsA acts as a ferritin receptor causing ferritin aggregation on the bacterial surface. Isothermal titration calorimetry data indicate that IlsA binds several types of ferritins through direct interaction with the shell subunits. UV-vis kinetic data show a significant enhancement of iron release from ferritin in the presence of IlsA indicating for the first time that a bacterial protein might alter the stability of the ferritin iron core. Disruption of the siderophore bacillibactin production drastically reduces the ability of B. cereus to utilize ferritin for growth and results in attenuated bacterial virulence in insects. We propose a new model of iron acquisition in B. cereus that involves the binding of IlsA to host ferritin followed by siderophore assisted iron uptake. Our results highlight a possible interplay between a surface protein and a siderophore and provide new insights into host adaptation of B. cereus and general bacterial pathogenesis. PMID:24550730

Symbiont acquisition as neoseme: origin of species and higher taxa

NASA Technical Reports Server (NTRS)

Bermudes, D.; Margulis, L.

1987-01-01

We examine the hypothesis that, in the origin of species and higher taxa of eukaryotes, symbiont acquisition followed by partner integration has been equivalent to neoseme appearance leading to speciation. The formation of stable symbiotic associations involves partner-surface recognition, behavioral and metabolic interaction, and, in some cases, gene product (RNA, protein) and genic (RNA, DNA) integration. This analysis is applied here to examples of neosemes that define specific taxa and to neosemes in plants, fungi, and animals that involve the appearance of new types of tissue. If this hypothesis is correct--if the origin of major genetic variation leading to speciation and even higher taxa may occur through symbiont acquisition and integration--then the analysis of "origins of species and higher taxa" becomes analogous to the study of microbial community ecology.

Proteomic Analysis of Embryogenesis and the Acquisition of Seed Dormancy in Norway Maple (Acer platanoides L.)

PubMed Central

Staszak, Aleksandra Maria; Pawłowski, Tomasz Andrzej

2014-01-01

The proteome of zygotic embryos of Acer platanoides L. was analyzed via high-resolution 2D-SDS-PAGE and MS/MS in order to: (1) identify significant physiological processes associated with embryo development; and (2) identify changes in the proteome of the embryo associated with the acquisition of seed dormancy. Seventeen spots were identified as associated with morphogenesis at 10 to 13 weeks after flowering (WAF). Thirty-three spots were associated with maturation of the embryo at 14 to 22 WAF. The greatest changes in protein abundance occurred at 22 WAF, when seeds become fully mature. Overall, the stage of morphogenesis was characterized by changes in the abundance of proteins (tubulins and actin) associated with the growth and development of the embryo. Enzymes related to energy supply were especially elevated, most likely due to the energy demand associated with rapid growth and cell division. The stage of maturation is crucial to the establishment of seed dormancy and is associated with a higher abundance of proteins involved in genetic information processing, energy and carbon metabolism and cellular and antioxidant processes. Results indicated that a glycine-rich RNA-binding protein and proteasome proteins may be directly involved in dormancy acquisition control, and future studies are warranted to verify this association. PMID:24941250

Proteomic analysis of embryogenesis and the acquisition of seed dormancy in Norway maple (Acer platanoides L.).

PubMed

Staszak, Aleksandra Maria; Pawłowski, Tomasz Andrzej

2014-06-17

The proteome of zygotic embryos of Acer platanoides L. was analyzed via high-resolution 2D-SDS-PAGE and MS/MS in order to: (1) identify significant physiological processes associated with embryo development; and (2) identify changes in the proteome of the embryo associated with the acquisition of seed dormancy. Seventeen spots were identified as associated with morphogenesis at 10 to 13 weeks after flowering (WAF). Thirty-three spots were associated with maturation of the embryo at 14 to 22 WAF. The greatest changes in protein abundance occurred at 22 WAF, when seeds become fully mature. Overall, the stage of morphogenesis was characterized by changes in the abundance of proteins (tubulins and actin) associated with the growth and development of the embryo. Enzymes related to energy supply were especially elevated, most likely due to the energy demand associated with rapid growth and cell division. The stage of maturation is crucial to the establishment of seed dormancy and is associated with a higher abundance of proteins involved in genetic information processing, energy and carbon metabolism and cellular and antioxidant processes. Results indicated that a glycine-rich RNA-binding protein and proteasome proteins may be directly involved in dormancy acquisition control, and future studies are warranted to verify this association.

Lactoferrin binding protein B – a bi-functional bacterial receptor protein

PubMed Central

Ostan, Nicholas K. H.; Yu, Rong-Hua; Ng, Dixon; Lai, Christine Chieh-Lin; Sarpe, Vladimir; Schriemer, David C.

2017-01-01

Lactoferrin binding protein B (LbpB) is a bi-lobed outer membrane-bound lipoprotein that comprises part of the lactoferrin (Lf) receptor complex in Neisseria meningitidis and other Gram-negative pathogens. Recent studies have demonstrated that LbpB plays a role in protecting the bacteria from cationic antimicrobial peptides due to large regions rich in anionic residues in the C-terminal lobe. Relative to its homolog, transferrin-binding protein B (TbpB), there currently is little evidence for its role in iron acquisition and relatively little structural and biophysical information on its interaction with Lf. In this study, a combination of crosslinking and deuterium exchange coupled to mass spectrometry, information-driven computational docking, bio-layer interferometry, and site-directed mutagenesis was used to probe LbpB:hLf complexes. The formation of a 1:1 complex of iron-loaded Lf and LbpB involves an interaction between the Lf C-lobe and LbpB N-lobe, comparable to TbpB, consistent with a potential role in iron acquisition. The Lf N-lobe is also capable of binding to negatively charged regions of the LbpB C-lobe and possibly other sites such that a variety of higher order complexes are formed. Our results are consistent with LbpB serving dual roles focused primarily on iron acquisition when exposed to limited levels of iron-loaded Lf on the mucosal surface and effectively binding apo Lf when exposed to high levels at sites of inflammation. PMID:28257520

Nutrient Acquisition and Metabolism by Campylobacter jejuni

PubMed Central

Stahl, Martin; Butcher, James; Stintzi, Alain

2012-01-01

The gastrointestinal pathogen Campylobacter jejuni is able to colonize numerous different hosts and compete against the gut microbiota. To do this, it must be able to efficiently acquire sufficient nutrients from its environment to support its survival and rapid growth in the intestine. However, despite almost 50 years of research, many aspects as to how C. jejuni accomplishes this feat remain poorly understood. C. jejuni lacks many of the common metabolic pathways necessary for the use of glucose, galactose, or other carbohydrates upon which most other microbes thrive. It does however make efficient use of citric acid cycle intermediates and various amino acids. C. jejuni readily uses the amino acids aspartate, glutamate, serine, and proline, with certain strains also possessing additional pathways allowing for the use of glutamine and asparagine. More recent work has revealed that some C. jejuni strains can metabolize the sugar l-fucose. This finding has upset years of dogma that C. jejuni is an asaccharolytic organism. C. jejuni also possesses diverse mechanisms for the acquisition of various transition metals that are required for metabolic activities. In particular, iron acquisition is critical for the formation of iron–sulfur complexes. C. jejuni is also unique in possessing both molybdate and tungsten cofactored proteins and thus has an unusual regulatory scheme for these metals. Together these various metabolic and acquisition pathways help C. jejuni to compete and thrive in wide variety of hosts and environments. PMID:22919597

75 FR 48872 - General Services Administration Acquisition Regulation; Rewrite of GSAR Part 541, Acquisition of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-12

... Acquisition Regulation; Rewrite of GSAR Part 541, Acquisition of Utility Services AGENCIES: Office of Acquisition Policy, General Services Administration (GSA). ACTION: Final rule. SUMMARY: The General Services Administration (GSA) is amending the General Services Administration Acquisition Regulation (GSAR) to improve the...

Excessive Acquisition in Hoarding

PubMed Central

Frost, Randy O.; Tolin, David F.; Steketee, Gail; Fitch, Kristin E.; Selbo-Bruns, Alexandra

2009-01-01

Compulsive hoarding (the acquisition of and failure to discard large numbers of possessions) is associated with substantial health risk, impairment, and economic burden. However, little research has examined separate components of this definition, particularly excessive acquisition. The present study examined acquisition in hoarding. Participants, 878 self-identified with hoarding and 665 family informants (not matched to hoarding participants), completed an internet survey. Among hoarding participants who met criteria for clinically significant hoarding, 61% met criteria for a diagnosis of compulsive buying and approximately 85% reported excessive acquisition. Family informants indicated that nearly 95% exhibited excessive acquisition. Those who acquired excessively had more severe hoarding; their hoarding had an earlier onset and resulted in more psychiatric work impairment days; and they experienced more symptoms of obsessive-compulsive disorder, depression, and anxiety. Two forms of excessive acquisition (buying and free things) each contributed independent variance in the prediction of hoarding severity and related symptoms. PMID:19261435

48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

48 CFR 46.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 46.404 Section 46.404 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance...

48 CFR 46.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 46.404 Section 46.404 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance...

48 CFR 46.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 46.404 Section 46.404 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance...

48 CFR 46.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 46.404 Section 46.404 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance...

48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section 246.404 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE...

48 CFR 46.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. 46.404 Section 46.404 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance...

The structure of lactoferrin-binding protein B from Neisseria meningitidis suggests roles in iron acquisition and neutralization of host defences

PubMed Central

Brooks, Cory L.; Arutyunova, Elena; Lemieux, M. Joanne

2014-01-01

Pathogens have evolved a range of mechanisms to acquire iron from the host during infection. Several Gram-negative pathogens including members of the genera Neisseria and Moraxella have evolved two-component systems that can extract iron from the host glycoproteins lactoferrin and transferrin. The homologous iron-transport systems consist of a membrane-bound transporter and an accessory lipoprotein. While the mechanism behind iron acquisition from transferrin is well understood, relatively little is known regarding how iron is extracted from lactoferrin. Here, the crystal structure of the N-terminal domain (N-lobe) of the accessory lipoprotein lactoferrin-binding protein B (LbpB) from the pathogen Neisseria meningitidis is reported. The structure is highly homologous to the previously determined structures of the accessory lipoprotein transferrin-binding protein B (TbpB) and LbpB from the bovine pathogen Moraxella bovis. Docking the LbpB structure with lactoferrin reveals extensive binding interactions with the N1 subdomain of lactoferrin. The nature of the interaction precludes apolactoferrin from binding LbpB, ensuring the specificity of iron-loaded lactoferrin. The specificity of LbpB safeguards proper delivery of iron-bound lactoferrin to the transporter lactoferrin-binding protein A (LbpA). The structure also reveals a possible secondary role for LbpB in protecting the bacteria from host defences. Following proteolytic digestion of lactoferrin, a cationic peptide derived from the N-terminus is released. This peptide, called lactoferricin, exhibits potent antimicrobial effects. The docked model of LbpB with lactoferrin reveals that LbpB interacts extensively with the N-terminal lactoferricin region. This may provide a venue for preventing the production of the peptide by proteolysis, or directly sequestering the peptide, protecting the bacteria from the toxic effects of lactoferricin. PMID:25286931

76 FR 39243 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-53; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-05

... ADMINISTRATION 48 CFR Chapter 1 [Docket FAR 2011-0077, Sequence 5] Federal Acquisition Regulation; Federal Acquisition Circular 2005-53; Small Entity Compliance Guide AGENCY: Department of Defense (DoD), General... Federal Acquisition Circular (FAC) 2005-53, which amend the Federal Acquisition Regulation (FAR). An...

Coordinating Council. Seventh Meeting: Acquisitions

NASA Technical Reports Server (NTRS)

1992-01-01

The theme for this NASA Scientific and Technical Information Program Coordinating Council meeting was Acquisitions. In addition to NASA and the NASA Center for AeroSpace Information (CASI) presentations, the report contains fairly lengthy visuals about acquisitions at the Defense Technical Information Center. CASI's acquisitions program and CASI's proactive acquisitions activity were described. There was a presentation on the document evaluation process at CASI. A talk about open literature scope and coverage at the American Institute of Aeronautics and Astronautics was also given. An overview of the STI Program's Acquisitions Experts Committee was given next. Finally acquisitions initiatives of the NASA STI program were presented.

48 CFR 304.7001 - Numbering acquisitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Numbering acquisitions. 304.7001 Section 304.7001 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATIVE MATTERS Acquisition Instrument Identification Numbering System 304.7001 Numbering acquisitions. (a...

Orphan spin operators enable the acquisition of multiple 2D and 3D magic angle spinning solid-state NMR spectra

NASA Astrophysics Data System (ADS)

Gopinath, T.; Veglia, Gianluigi

2013-05-01

We propose a general method that enables the acquisition of multiple 2D and 3D solid-state NMR spectra for U-13C, 15N-labeled proteins. This method, called MEIOSIS (Multiple ExperIments via Orphan SpIn operatorS), makes it possible to detect four coherence transfer pathways simultaneously, utilizing orphan (i.e., neglected) spin operators of nuclear spin polarization generated during 15N-13C cross polarization (CP). In the MEIOSIS experiments, two phase-encoded free-induction decays are decoded into independent nuclear polarization pathways using Hadamard transformations. As a proof of principle, we show the acquisition of multiple 2D and 3D spectra of U-13C, 15N-labeled microcrystalline ubiquitin. Hadamard decoding of CP coherences into multiple independent spin operators is a new concept in solid-state NMR and is extendable to many other multidimensional experiments. The MEIOSIS method will increase the throughput of solid-state NMR techniques for microcrystalline proteins, membrane proteins, and protein fibrils.

In-depth evaluation of software tools for data-independent acquisition based label-free quantification.

PubMed

Kuharev, Jörg; Navarro, Pedro; Distler, Ute; Jahn, Olaf; Tenzer, Stefan

2015-09-01

Label-free quantification (LFQ) based on data-independent acquisition workflows currently experiences increasing popularity. Several software tools have been recently published or are commercially available. The present study focuses on the evaluation of three different software packages (Progenesis, synapter, and ISOQuant) supporting ion mobility enhanced data-independent acquisition data. In order to benchmark the LFQ performance of the different tools, we generated two hybrid proteome samples of defined quantitative composition containing tryptically digested proteomes of three different species (mouse, yeast, Escherichia coli). This model dataset simulates complex biological samples containing large numbers of both unregulated (background) proteins as well as up- and downregulated proteins with exactly known ratios between samples. We determined the number and dynamic range of quantifiable proteins and analyzed the influence of applied algorithms (retention time alignment, clustering, normalization, etc.) on quantification results. Analysis of technical reproducibility revealed median coefficients of variation of reported protein abundances below 5% for MS(E) data for Progenesis and ISOQuant. Regarding accuracy of LFQ, evaluation with synapter and ISOQuant yielded superior results compared to Progenesis. In addition, we discuss reporting formats and user friendliness of the software packages. The data generated in this study have been deposited to the ProteomeXchange Consortium with identifier PXD001240 (http://proteomecentral.proteomexchange.org/dataset/PXD001240). © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

48 CFR 3034.004 - Acquisition strategy.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Acquisition strategy. 3034.004 Section 3034.004 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY, HOMELAND... Acquisition strategy. See (HSAR) 48 CFR 3009.570 for policy applicable to acquisition strategies that consider...

48 CFR 34.004 - Acquisition strategy.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisition strategy. 34... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 34.004 Acquisition strategy. The program manager, as specified in agency procedures, shall develop an acquisition strategy tailored to the particular...

Performance-Based Services Acquisition

DTIC Science & Technology

2011-02-01

47  DoD’s acquisition workforce lacks training and experience in services contracting ... 47  Selecting correct metrics...services more effectively; vii (2) the DoD’s acquisition workforce lacks training and experience in services contracting; (3) selecting correct...private sector; (2) improve the training of government services acquisition personnel; and (3) the USD(AT&L) should incentivize the existing workforce

76 FR 21849 - Defense Federal Acquisition Regulation Supplement; Ships Bunkers Easy Acquisition (SEA) Card® and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-19

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 213 RIN 0750-AH07 Defense Federal Acquisition Regulation Supplement; Ships Bunkers Easy Acquisition (SEA) Card[supreg] and Aircraft Ground Services (DFARS Case 2009-D019) AGENCY: Defense Acquisition Regulations System, Department of...

76 FR 58149 - Defense Federal Acquisition Regulation Supplement; Ships Bunkers Easy Acquisition (SEA) Card® and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-20

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 213 RIN 0750-AH07 Defense Federal Acquisition Regulation Supplement; Ships Bunkers Easy Acquisition (SEA) Card[supreg] and Aircraft Ground Services (DFARS Case 2009-D019) AGENCY: Defense Acquisition Regulations System, Department of...

Introduction to Defense Acquisition Management

DTIC Science & Technology

1989-03-01

the system after Most new systems follow the same formatted and its usefullness in the weapon inventory predictable life cycle, and fit the model...natives for system concept development Statement (MNS) setting forth requirements need- -An acquisition strategy is developed to guide ed to meet the...6 BUSINESS, FINANCIAL AND TECHNICAL ASPECTS OF SYSTEMS ACQUISITION Management of the systems acquisition process The acquisition planning phase of the

Comprehensible Output, From Occurrence to Acquisition: An Agenda for Acquisitional Research.

ERIC Educational Resources Information Center

Shehadeh, Ali

2002-01-01

Proposes a research agenda that makes acquisitional research central to the study of comprehensible output (CO). Looks at the context in which the CO hypothesis was proposed--that of looking beyond comprehensible input as a condition for second language acquisition. (Author/VWL)

48 CFR 2419.804-370 - SBA acceptance under partnership agreements for acquisitions exceeding the simplified acquisition...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false SBA acceptance under partnership agreements for acquisitions exceeding the simplified acquisition threshold. 2419.804-370 Section 2419.804-370 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS...

48 CFR 2419.804-370 - SBA acceptance under partnership agreements for acquisitions exceeding the simplified acquisition...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false SBA acceptance under partnership agreements for acquisitions exceeding the simplified acquisition threshold. 2419.804-370 Section 2419.804-370 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS...

Image Acquisition Context

PubMed Central

Bidgood, W. Dean; Bray, Bruce; Brown, Nicolas; Mori, Angelo Rossi; Spackman, Kent A.; Golichowski, Alan; Jones, Robert H.; Korman, Louis; Dove, Brent; Hildebrand, Lloyd; Berg, Michael

1999-01-01

Objective: To support clinically relevant indexing of biomedical images and image-related information based on the attributes of image acquisition procedures and the judgments (observations) expressed by observers in the process of image interpretation. Design: The authors introduce the notion of “image acquisition context,” the set of attributes that describe image acquisition procedures, and present a standards-based strategy for utilizing the attributes of image acquisition context as indexing and retrieval keys for digital image libraries. Methods: The authors' indexing strategy is based on an interdependent message/terminology architecture that combines the Digital Imaging and Communication in Medicine (DICOM) standard, the SNOMED (Systematized Nomenclature of Human and Veterinary Medicine) vocabulary, and the SNOMED DICOM microglossary. The SNOMED DICOM microglossary provides context-dependent mapping of terminology to DICOM data elements. Results: The capability of embedding standard coded descriptors in DICOM image headers and image-interpretation reports improves the potential for selective retrieval of image-related information. This favorably affects information management in digital libraries. PMID:9925229

Data acquisition system

DOEpatents

Shapiro, Stephen L.; Mani, Sudhindra; Atlas, Eugene L.; Cords, Dieter H. W.; Holbrook, Britt

1997-01-01

A data acquisition circuit for a particle detection system that allows for time tagging of particles detected by the system. The particle detection system screens out background noise and discriminate between hits from scattered and unscattered particles. The detection system can also be adapted to detect a wide variety of particle types. The detection system utilizes a particle detection pixel array, each pixel containing a back-biased PIN diode, and a data acquisition pixel array. Each pixel in the particle detection pixel array is in electrical contact with a pixel in the data acquisition pixel array. In response to a particle hit, the affected PIN diodes generate a current, which is detected by the corresponding data acquisition pixels. This current is integrated to produce a voltage across a capacitor, the voltage being related to the amount of energy deposited in the pixel by the particle. The current is also used to trigger a read of the pixel hit by the particle.

Discovery of potential protein biomarkers of lung adenocarcinoma in bronchoalveolar lavage fluid by SWATH MS data-independent acquisition and targeted data extraction.

PubMed

Ortea, I; Rodríguez-Ariza, A; Chicano-Gálvez, E; Arenas Vacas, M S; Jurado Gámez, B

2016-04-14

Lung cancer currently ranks as the neoplasia with the highest global mortality rate. Although some improvements have been introduced in recent years, new advances in diagnosis are required in order to increase survival rates. New mildly invasive endoscopy-based diagnostic techniques include the collection of bronchoalveolar lavage fluid (BALF), which is discarded after using a portion of the fluid for standard pathological procedures. BALF proteomic analysis can contribute to clinical practice with more sensitive biomarkers, and can complement cytohistological studies by aiding in the diagnosis, prognosis, and subtyping of lung cancer, as well as the monitoring of treatment response. The range of quantitative proteomics methodologies used for biomarker discovery is currently being broadened with the introduction of data-independent acquisition (DIA) analysis-related approaches that address the massive quantitation of the components of a proteome. Here we report for the first time a DIA-based quantitative proteomics study using BALF as the source for the discovery of potential lung cancer biomarkers. The results have been encouraging in terms of the number of identified and quantified proteins. A panel of candidate protein biomarkers for adenocarcinoma in BALF is reported; this points to the activation of the complement network as being strongly over-represented and suggests this pathway as a potential target for lung cancer research. In addition, the results reported for haptoglobin, complement C4-A, and glutathione S-transferase pi are consistent with previous studies, which indicates that these proteins deserve further consideration as potential lung cancer biomarkers in BALF. Our study demonstrates that the analysis of BALF proteins by liquid chromatography-tandem mass spectrometry (LC-MS/MS), combining a simple sample pre-treatment and SWATH DIA MS, is a useful method for the discovery of potential lung cancer biomarkers. Bronchoalveolar lavage fluid (BALF

[First language acquisition research and theories of language acquisition].

PubMed

Miller, S; Jungheim, M; Ptok, M

2014-04-01

In principle, a child can seemingly easily acquire any given language. First language acquisition follows a certain pattern which to some extent is found to be language independent. Since time immemorial, it has been of interest why children are able to acquire language so easily. Different disciplinary and methodological orientations addressing this question can be identified. A selective literature search in PubMed and Scopus was carried out and relevant monographies were considered. Different, partially overlapping phases can be distinguished in language acquisition research: whereas in ancient times, deprivation experiments were carried out to discover the "original human language", the era of diary studies began in the mid-19th century. From the mid-1920s onwards, behaviouristic paradigms dominated this field of research; interests were focussed on the determination of normal, average language acquisition. The subsequent linguistic period was strongly influenced by the nativist view of Chomsky and the constructivist concepts of Piaget. Speech comprehension, the role of speech input and the relevance of genetic disposition became the centre of attention. The interactionist concept led to a revival of the convergence theory according to Stern. Each of these four major theories--behaviourism, cognitivism, interactionism and nativism--have given valuable and unique impulses, but no single theory is universally accepted to provide an explanation of all aspects of language acquisition. Moreover, it can be critically questioned whether clinicians consciously refer to one of these theories in daily routine work and whether therapies are then based on this concept. It remains to be seen whether or not new theories of grammar, such as the so-called construction grammar (CxG), will eventually change the general concept of language acquisition.

Using Data Independent Acquisition (DIA) to Model High-responding Peptides for Targeted Proteomics Experiments*

PubMed Central

Searle, Brian C.; Egertson, Jarrett D.; Bollinger, James G.; Stergachis, Andrew B.; MacCoss, Michael J.

2015-01-01

Targeted mass spectrometry is an essential tool for detecting quantitative changes in low abundant proteins throughout the proteome. Although selected reaction monitoring (SRM) is the preferred method for quantifying peptides in complex samples, the process of designing SRM assays is laborious. Peptides have widely varying signal responses dictated by sequence-specific physiochemical properties; one major challenge is in selecting representative peptides to target as a proxy for protein abundance. Here we present PREGO, a software tool that predicts high-responding peptides for SRM experiments. PREGO predicts peptide responses with an artificial neural network trained using 11 minimally redundant, maximally relevant properties. Crucial to its success, PREGO is trained using fragment ion intensities of equimolar synthetic peptides extracted from data independent acquisition experiments. Because of similarities in instrumentation and the nature of data collection, relative peptide responses from data independent acquisition experiments are a suitable substitute for SRM experiments because they both make quantitative measurements from integrated fragment ion chromatograms. Using an SRM experiment containing 12,973 peptides from 724 synthetic proteins, PREGO exhibits a 40–85% improvement over previously published approaches at selecting high-responding peptides. These results also represent a dramatic improvement over the rules-based peptide selection approaches commonly used in the literature. PMID:26100116

Hsp90 shapes protein and RNA evolution to balance trade-offs between protein stability and aggregation.

PubMed

Geller, Ron; Pechmann, Sebastian; Acevedo, Ashley; Andino, Raul; Frydman, Judith

2018-05-03

Acquisition of mutations is central to evolution; however, the detrimental effects of most mutations on protein folding and stability limit protein evolvability. Molecular chaperones, which suppress aggregation and facilitate polypeptide folding, may alleviate the effects of destabilizing mutations thus promoting sequence diversification. To illuminate how chaperones can influence protein evolution, we examined the effect of reduced activity of the chaperone Hsp90 on poliovirus evolution. We find that Hsp90 offsets evolutionary trade-offs between protein stability and aggregation. Lower chaperone levels favor variants of reduced hydrophobicity and protein aggregation propensity but at a cost to protein stability. Notably, reducing Hsp90 activity also promotes clusters of codon-deoptimized synonymous mutations at inter-domain boundaries, likely to facilitate cotranslational domain folding. Our results reveal how a chaperone can shape the sequence landscape at both the protein and RNA levels to harmonize competing constraints posed by protein stability, aggregation propensity, and translation rate on successful protein biogenesis.

48 CFR 307.7106 - Acquisition milestones.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Acquisition milestones. 307.7106 Section 307.7106 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES COMPETITION... to track progress of the acquisition. The milestone schedule signatories (see the Requirements and...

48 CFR 307.7106 - Acquisition milestones.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Acquisition milestones. 307.7106 Section 307.7106 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES COMPETITION... to track progress of the acquisition. The milestone schedule signatories (see the Requirements and...

[Effector proteins of Clamidia].

PubMed

Kariagina, A S; Alekseevskiĭ, A V; Spirin, S A; Zigangirova, N A; Gintsburg, A L

2009-01-01

The review summarizes the recent published data on molecular mechanisms of Chlamidiae - host cell interaction, first of all on chlamydial effector proteins. Such proteins as well as III transport system proteins that transfer many effector proteins into host cytoplasm are attractive targets for drug therapy of chlamydial infections. The majority of the data concerns two species, Chlamydia trachomatis and Chlamydophila pneumoniae. C. trachomatis protein TARP, which is presynthesized in elementary bodies, plays an essential role in the initial stages of the infection. Patogen proteins participating in the next stage, that is the intracellular inclusion traffic to the centrosome, are CT229 of C. trachomatis and Cpn0585 of C. pneumoniae, which interact with cellular Rab GTPases. In C. trachomatis, IncA protein plays a key role in chlamydial inclusions fusion, CT847 modulates life cycle of the host cell, LDA3 is essential in acquisition of nutrients. CPAF protease and inclusion membrane proteins IncG and CADD participate in suppression of apoptosis of infected cells. The proteases CPAF and CT441, as well as deubiquitinating ChlaDub1 protein, contribute to avoiding the immune response.

23 CFR 710.501 - Early acquisition.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Early acquisition. 710.501 Section 710.501 Highways... REAL ESTATE Property Acquisition Alternatives § 710.501 Early acquisition. (a) Real property... so based on program or project considerations. The State may undertake early acquisition for corridor...

23 CFR 710.501 - Early acquisition.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 23 Highways 1 2011-04-01 2011-04-01 false Early acquisition. 710.501 Section 710.501 Highways... REAL ESTATE Property Acquisition Alternatives § 710.501 Early acquisition. (a) Real property... so based on program or project considerations. The State may undertake early acquisition for corridor...

48 CFR 7.402 - Acquisition methods.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time when...

48 CFR 7.402 - Acquisition methods.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time when...

48 CFR 7.402 - Acquisition methods.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time when...

48 CFR 7.402 - Acquisition methods.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time when...

48 CFR 7.402 - Acquisition methods.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time when...

77 FR 73515 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-63; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-10

... Administration National Aeronautics and Space Administration 48 CFR Chapter 1 Federal Acquisition Regulations... AND SPACE ADMINISTRATION 48 CFR Chapter 1 [Docket FAR 2012-0080, Sequence 6] Federal Acquisition... Services Administration (GSA), and National Aeronautics and Space Administration (NASA). ACTION: Summary...

First Language Acquisition and Teaching

ERIC Educational Resources Information Center

Cruz-Ferreira, Madalena

2011-01-01

"First language acquisition" commonly means the acquisition of a single language in childhood, regardless of the number of languages in a child's natural environment. Language acquisition is variously viewed as predetermined, wondrous, a source of concern, and as developing through formal processes. "First language teaching" concerns schooling in…

Making Acquisition Measurable

DTIC Science & Technology

2011-04-30

Corporation. All rights reserved End Users Administrator/ Maintainer (A/M) Subject Matter Expert ( SME ) Trainer/ Instructor Manager, Evaluator, Supervisor... CMMI ) - Acquisition (AQ) © 2011 The MITRE Corporation. All rights reserved 13 CMMI -Development Incremental iterative development (planning & execution...objectives Constructing games highlighting particular aspects of proposed CCOD® acquisition, and conducting exercises with Subject Matter Experts ( SMEs

25 CFR 700.115 - Preliminary acquisition notice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Acquisition and Disposal of Habitations and/or Improvements § 700.115 Preliminary acquisition notice. As soon as feasible in the acquisition process, the Commission shall issue a preliminary acquisition notice.../her habitations and/or improvements. (b) Explain that such preliminary acquisition notice is not a...

48 CFR 908.7101-3 - Direct acquisition.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Direct acquisition. 908.7101-3 Section 908.7101-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY COMPETITION ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES AND SERVICES Acquisition of Special Items 908.7101-3 Direct...

2 CFR 200.88 - Simplified acquisition threshold.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false Simplified acquisition threshold. 200.88... acquisition threshold. Simplified acquisition threshold means the dollar amount below which a non-Federal... threshold. The simplified acquisition threshold is set by the Federal Acquisition Regulation at 48 CFR...

Unmanned Maritime Systems Incremental Acquisition Approach

DTIC Science & Technology

2016-12-01

We find that current UMS acquisitions are utilizing previous acquisition reforms, but could benefit from additional contractor peer competition and...peer review. Additional cost and schedule benefits could result from contractor competition during build processes in each incremental process. We...acquisitions are utilizing previous acquisition reforms, but could benefit from additional contractor peer competition and peer review. Additional

48 CFR 1.102-3 - Acquisition team.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Acquisition team. 1.102-3... ACQUISITION REGULATIONS SYSTEM Purpose, Authority, Issuance 1.102-3 Acquisition team. The purpose of defining the Federal Acquisition Team (Team) in the Guiding Principles is to ensure that participants in the...

48 CFR 1.102-3 - Acquisition team.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Acquisition team. 1.102-3... ACQUISITION REGULATIONS SYSTEM Purpose, Authority, Issuance 1.102-3 Acquisition team. The purpose of defining the Federal Acquisition Team (Team) in the Guiding Principles is to ensure that participants in the...

48 CFR 1.102-3 - Acquisition team.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Acquisition team. 1.102-3... ACQUISITION REGULATIONS SYSTEM Purpose, Authority, Issuance 1.102-3 Acquisition team. The purpose of defining the Federal Acquisition Team (Team) in the Guiding Principles is to ensure that participants in the...

48 CFR 1.102-3 - Acquisition team.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Acquisition team. 1.102-3... ACQUISITION REGULATIONS SYSTEM Purpose, Authority, Issuance 1.102-3 Acquisition team. The purpose of defining the Federal Acquisition Team (Team) in the Guiding Principles is to ensure that participants in the...

48 CFR 1.102-3 - Acquisition team.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisition team. 1.102-3... ACQUISITION REGULATIONS SYSTEM Purpose, Authority, Issuance 1.102-3 Acquisition team. The purpose of defining the Federal Acquisition Team (Team) in the Guiding Principles is to ensure that participants in the...

Multi-echo acquisition

PubMed Central

Posse, Stefan

2011-01-01

The rapid development of fMRI was paralleled early on by the adaptation of MR spectroscopic imaging (MRSI) methods to quantify water relaxation changes during brain activation. This review describes the evolution of multi-echo acquisition from high-speed MRSI to multi-echo EPI and beyond. It highlights milestones in the development of multi-echo acquisition methods, such as the discovery of considerable gains in fMRI sensitivity when combining echo images, advances in quantification of the BOLD effect using analytical biophysical modeling and interleaved multi-region shimming. The review conveys the insight gained from combining fMRI and MRSI methods and concludes with recent trends in ultra-fast fMRI, which will significantly increase temporal resolution of multi-echo acquisition. PMID:22056458

75 FR 54524 - Defense Federal Acquisition Regulation Supplement; Acquisition Strategies To Ensure Competition...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-08

... Life Cycle of Major Defense Acquisition Programs (DFARS Case 2009-D014) AGENCY: Defense Acquisition... competition at both the prime contract and subcontract level of the MDAP throughout its life cycle as a means...

Comparison of Data Acquisition Strategies on Quadrupole Ion Trap Instrumentation for Shotgun Proteomics

PubMed Central

Canterbury, Jesse D.; Merrihew, Gennifer E.; Goodlett, David R.; MacCoss, Michael J.; Shaffer, Scott A.

2015-01-01

A common strategy in mass spectrometry analyses of complex protein mixtures is to digest the proteins to peptides, separate the peptides by microcapillary liquid chromatography and collect tandem mass spectra (MS/MS) on the eluting, complex peptide mixtures, a process commonly termed “shotgun proteomics”. For years, the most common way of data collection was via data-dependent acquisition (DDA), a process driven by an automated instrument control routine that directs MS/MS acquisition from the highest abundant signals to the lowest, a process often leaving lower abundant signals unanalyzed and therefore unidentified in the experiment. Advances in both instrumentation duty cycle and sensitivity allow DDA to probe to lower peptide abundance and therefore enable mapping proteomes to a more significant depth. An alternative to acquiring data by DDA is by data-independent acquisition (DIA), in which a specified range in m/z is fragmented without regard to prioritization of a precursor ion or its relative abundance in the mass spectrum. As a consequence, DIA acquisition potentially offers more comprehensive analysis of peptides than DDA and in principle can yield tandem mass spectra of all ionized molecules following their conversion to the gas-phase. In this work, we evaluate both DDA and DIA on three different linear ion trap instruments: an LTQ, an LTQ modified in-house with an electrodynamic ion funnel, and an LTQ-Velos. These instruments were chosen as they are representative of both older (LTQ) and newer (LTQ-Velos) ion trap designs i.e., linear ion trap and dual ion traps, respectively, and allow direct comparison of peptide identification using both DDA and DIA analysis. Further, as the LTQ-Velos has an improved “S-lens” ion guide in the high-pressure region to improve ion flux, we found it logical to determine if the former LTQ model could be leveraged by improving sensitivity by modifying with an electrodynamic ion guide of significantly different

Technical advances in proteomics: new developments in data-independent acquisition.

PubMed

Hu, Alex; Noble, William S; Wolf-Yadlin, Alejandro

2016-01-01

The ultimate aim of proteomics is to fully identify and quantify the entire complement of proteins and post-translational modifications in biological samples of interest. For the last 15 years, liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data-dependent acquisition (DDA) mode has been the standard for proteomics when sampling breadth and discovery were the main objectives; multiple reaction monitoring (MRM) LC-MS/MS has been the standard for targeted proteomics when precise quantification, reproducibility, and validation were the main objectives. Recently, improvements in mass spectrometer design and bioinformatics algorithms have resulted in the rediscovery and development of another sampling method: data-independent acquisition (DIA). DIA comprehensively and repeatedly samples every peptide in a protein digest, producing a complex set of mass spectra that is difficult to interpret without external spectral libraries. Currently, DIA approaches the identification breadth of DDA while achieving the reproducible quantification characteristic of MRM or its newest version, parallel reaction monitoring (PRM). In comparative de novo identification and quantification studies in human cell lysates, DIA identified up to 89% of the proteins detected in a comparable DDA experiment while providing reproducible quantification of over 85% of them. DIA analysis aided by spectral libraries derived from prior DIA experiments or auxiliary DDA data produces identification and quantification as reproducible and precise as that achieved by MRM/PRM, except on low‑abundance peptides that are obscured by stronger signals. DIA is still a work in progress toward the goal of sensitive, reproducible, and precise quantification without external spectral libraries. New software tools applied to DIA analysis have to deal with deconvolution of complex spectra as well as proper filtering of false positives and false negatives. However, the future outlook is positive, and various

Acquisition Strategy Guide,

DTIC Science & Technology

1984-07-01

acquisition stategy for his program. Well informed, educated, and innovative applications and judgments are necessary to structure a successful...program but part of’the Con- operating forces. gress is opposed to it. A case in point is thle MIX missile development. In other instances the Ad...hierarchical format, can be applied to help in The market factors element includes both industrial organizing and structuring an acquisition strategy

Relationships among measurements obtained by use of computed tomography and radiography and scores of cartilage microdamage in hip joints with moderate to severe joint laxity of adult dogs.

PubMed

Lopez, Mandi J; Lewis, Brooke P; Swaab, Megan E; Markel, Mark D

2008-03-01

To evaluate correlations among measurements on radiographic and computed tomography (CT) images with articular cartilage microdamage in lax hip joints of dogs. 12 adult mixed-breed hounds. Pelvic CT and radiography were performed. Hip joints were harvested following euthanasia. Orthopedic Foundation for Animals (OFA) and PennHIP radiograph reports were obtained. Norberg angle (NA) and radiographic percentage femoral head coverage (RPC) were determined. Center-edge angle (CEA), horizontal toit externe angle (HTEA), ventral acetabular sector angle (VASA), dorsal acetabular sector angle (DASA), horizontal acetabular sector angle (HASA), acetabular index (AI), and CT percentage femoral head coverage (CPC) were measured on 2-dimensional CT images. Femoral head-acetabular shelf percentage was measured on sagittal 3-dimensional CT (SCT) and transverse 3-dimensional CT (TCT) images. Light microscopy was used to score joint cartilage. Relationships of OFA confirmation and PennHIP osteoarthritis scores with radiography, CT, and cartilage variables and relationships of cartilage scores with radiography and CT measurements were evaluated with Spearman rank correlations. Pearson correlation was used for relationships of distraction index (DI) with radiography, CT, and cartilage variables. Significant relationships included PennHIP osteoarthritis score with cartilage score, CEA, HTEA, DASA, AI, CPC, and TCT; OFA confirmation score with cartilage score, NA, RPC, CEA, HTEA, DASA, AI, CPC, and TCT; cartilage score with NA, RPC, CEA, HTEA, DASA, HASA, AI, and TCT; and DI with cartilage score, CEA, HTEA, DASA, HASA, AI, and CPC. CT appeared to be a valuable imaging modality for predicting cartilage microdamage in canine hip joints.

48 CFR 434.004 - Acquisition strategy.

Code of Federal Regulations, 2010 CFR

2010-10-01

....004 Section 434.004 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SPECIAL... manager will develop, in coordination with the Acquisition Executive or Major Information Technology... approval of the Acquisition Executive or Major Information Technology Systems Executive, a project control...

48 CFR 252.211-7000 - Acquisition streamlining.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Acquisition streamlining. 252.211-7000 Section 252.211-7000 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text...

Blueprint for Acquisition Reform, Version 3.0

DTIC Science & Technology

2008-07-01

represents a substantial and immediate step forward in establishing the Coast Guard as a model mid-sized federal agency for acquisition processes...Blueprint for Acquisition Reform in the U. S. Coast Guard “The Coast Guard must become the model for mid-sized Federal agency acquisition in process...acquisition (DoD 5000 model >CG Major Systems Acquisition Manual) • Deepwater Program Executive Officer (PEO): System of Systems performance-based

MODELING INACTIVATION OF GIARDIA LAMBLIA

EPA Science Inventory

Under the auspices of the Safe Drinking Water Act (SDWA)the U.S. EPA hasa promulgated the Surface Water Treatment Rule (SWTR) requiring public water systems using surface water to provide minimum disinfection to Control Giardia Lamblia, enteric virsues, and bacteria. The C-t con...

48 CFR 237.7001 - Method of acquisition.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Method of acquisition. 237.7001 Section 237.7001 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM... contract exists, use DD Form 1155, Order for Supplies or Services, to obtain mortuary services. ...

Detection of amide I signals of interfacial proteins in situ using SFG.

PubMed

Wang, Jie; Even, Mark A; Chen, Xiaoyun; Schmaier, Alvin H; Waite, J Herbert; Chen, Zhan

2003-08-20

In this Communication, we demonstrate the novel observation that it is feasible to collect amide signals from polymer/protein solution interfaces in situ using sum frequency generation (SFG) vibrational spectroscopy. Such SFG amide signals allow for acquisition of more detailed molecular level information of entire interfacial protein structures. Proteins investigated include bovine serum albumin, mussel protein mefp-2, factor XIIa, and ubiquitin. Our studies indicate that different proteins generate different SFG amide signals at the polystyrene/protein solution interface, showing that they have different interfacial coverage, secondary structure, or orientation.

Shared and distinct mechanisms of iron acquisition by bacterial and fungal pathogens of humans

PubMed Central

Caza, Mélissa; Kronstad, James W.

2013-01-01

Iron is the most abundant transition metal in the human body and its bioavailability is stringently controlled. In particular, iron is tightly bound to host proteins such as transferrin to maintain homeostasis, to limit potential damage caused by iron toxicity under physiological conditions and to restrict access by pathogens. Therefore, iron acquisition during infection of a human host is a challenge that must be surmounted by every successful pathogenic microorganism. Iron is essential for bacterial and fungal physiological processes such as DNA replication, transcription, metabolism, and energy generation via respiration. Hence, pathogenic bacteria and fungi have developed sophisticated strategies to gain access to iron from host sources. Indeed, siderophore production and transport, iron acquisition from heme and host iron-containing proteins such as hemoglobin and transferrin, and reduction of ferric to ferrous iron with subsequent transport are all strategies found in bacterial and fungal pathogens of humans. This review focuses on a comparison of these strategies between bacterial and fungal pathogens in the context of virulence and the iron limitation that occurs in the human body as a mechanism of innate nutritional defense. PMID:24312900

75 FR 8272 - Defense Federal Acquisition Regulation Supplement; Acquisition Strategies To Ensure Competition...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-24

... Competition Throughout the Life Cycle of Major Defense Acquisition Programs AGENCY: Defense Acquisition... subcontract level of the MDAP throughout its life cycle as a means to improve contractor performance; and (2...) throughout the program life cycle as a means to improve contractor performance; and (ii) Document the...

Human target acquisition performance

NASA Astrophysics Data System (ADS)

Teaney, Brian P.; Du Bosq, Todd W.; Reynolds, Joseph P.; Thompson, Roger; Aghera, Sameer; Moyer, Steven K.; Flug, Eric; Espinola, Richard; Hixson, Jonathan

2012-06-01

The battlefield has shifted from armored vehicles to armed insurgents. Target acquisition (identification, recognition, and detection) range performance involving humans as targets is vital for modern warfare. The acquisition and neutralization of armed insurgents while at the same time minimizing fratricide and civilian casualties is a mounting concern. U.S. Army RDECOM CERDEC NVESD has conducted many experiments involving human targets for infrared and reflective band sensors. The target sets include human activities, hand-held objects, uniforms & armament, and other tactically relevant targets. This paper will define a set of standard task difficulty values for identification and recognition associated with human target acquisition performance.

Data Acquisition for Modular Biometric Monitoring System

NASA Technical Reports Server (NTRS)

Grodsinsky, Carlos M. (Inventor); Chmiel, Alan J. (Inventor); Humphreys, Bradley T. (Inventor)

2014-01-01

A modular system for acquiring biometric data includes a plurality of data acquisition modules configured to sample biometric data from at least one respective input channel at a data acquisition rate. A representation of the sampled biometric data is stored in memory of each of the plurality of data acquisition modules. A central control system is in communication with each of the plurality of data acquisition modules through a bus. The central control system is configured to collect data asynchronously, via the bus, from the memory of the plurality of data acquisition modules according to a relative fullness of the memory of the plurality of data acquisition modules.

14 CFR 151.73 - Land acquisition.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition of...

14 CFR 151.73 - Land acquisition.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition of...

14 CFR 151.73 - Land acquisition.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition of...

14 CFR 151.73 - Land acquisition.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition of...

14 CFR 151.73 - Land acquisition.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition of...

76 FR 10476 - (General Provisions) Contract Appeals and the Acquisition Regulation: General, Acquisition...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-25

... DEPARTMENT OF ENERGY 10 CFR Part 1023 48 CFR Parts 901, 902, 903, 904, 906, 907, 908, 909, 911, 914, 915, 916, 917, and 952 RIN 1991-AB81 (General Provisions) Contract Appeals and the Acquisition Regulation: General, Acquisition Planning, and Contracting Methods and Contract Types Correction In rule...

7 CFR 1735.20 - Acquisitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... GENERAL POLICIES, TYPES OF LOANS, LOAN REQUIREMENTS-TELECOMMUNICATIONS PROGRAM Loan Purposes and Basic Policies § 1735.20 Acquisitions. (a) RUS finances the acquisition by a borrower of another system, lines...

32 CFR 644.7 - Acquisition lines.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 4 2011-07-01 2011-07-01 false Acquisition lines. 644.7 Section 644.7 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Project Planning Civil Works § 644.7 Acquisition lines. (a) Tentative acquisition lines. As...

75 FR 43090 - Federal Acquisition Regulation; Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-23

... Acquisition Regulation; Correction AGENCY: Department of Defense (DoD), General Services Administration (GSA... Civilian Agency Acquisition Council and the Defense Acquisition Regulations Council are issuing a...

2 CFR 200.2 - Acquisition cost.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false Acquisition cost. 200.2 Section 200.2 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT... REQUIREMENTS FOR FEDERAL AWARDS Acronyms and Definitions Acronyms § 200.2 Acquisition cost. Acquisition cost...

7 CFR 1735.72 - Acquisition agreements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 11 2014-01-01 2014-01-01 false Acquisition agreements. 1735.72 Section 1735.72... Involving Loan Funds § 1735.72 Acquisition agreements. When borrowers are seeking RUS financing, acquisition agreements between the borrower and the seller must be in form and substance satisfactory to RUS and shall be...

Drosophila Araucan and Caupolican Integrate Intrinsic and Signalling Inputs for the Acquisition by Muscle Progenitors of the Lateral Transverse Fate

PubMed Central

Carrasco-Rando, Marta; Tutor, Antonio S.; Prieto-Sánchez, Silvia; González-Pérez, Esther; Barrios, Natalia; Letizia, Annalisa; Martín, Paloma; Campuzano, Sonsoles; Ruiz-Gómez, Mar

2011-01-01

A central issue of myogenesis is the acquisition of identity by individual muscles. In Drosophila, at the time muscle progenitors are singled out, they already express unique combinations of muscle identity genes. This muscle code results from the integration of positional and temporal signalling inputs. Here we identify, by means of loss-of-function and ectopic expression approaches, the Iroquois Complex homeobox genes araucan and caupolican as novel muscle identity genes that confer lateral transverse muscle identity. The acquisition of this fate requires that Araucan/Caupolican repress other muscle identity genes such as slouch and vestigial. In addition, we show that Caupolican-dependent slouch expression depends on the activation state of the Ras/Mitogen Activated Protein Kinase cascade. This provides a comprehensive insight into the way Iroquois genes integrate in muscle progenitors, signalling inputs that modulate gene expression and protein activity. PMID:21811416

The OncoPPi Portal: an integrative resource to explore and prioritize protein-protein interactions for cancer target discovery. | Office of Cancer Genomics

Cancer.gov

Motivation: As cancer genomics initiatives move toward comprehensive identification of genetic alterations in cancer, attention is now turning to understanding how interactions among these genes lead to the acquisition of tumor hallmarks. Emerging pharmacological and clinical data suggest a highly promising role of cancer-specific protein-protein interactions (PPIs) as druggable cancer targets. However, large-scale experimental identification of cancer-related PPIs remains challenging, and currently available resources to explore oncogenic PPI networks are limited.

Microcomputer data acquisition and control.

PubMed

East, T D

1986-01-01

In medicine and biology there are many tasks that involve routine well defined procedures. These tasks are ideal candidates for computerized data acquisition and control. As the performance of microcomputers rapidly increases and cost continues to go down the temptation to automate the laboratory becomes great. To the novice computer user the choices of hardware and software are overwhelming and sadly most of the computer sales persons are not at all familiar with real-time applications. If you want to bill your patients you have hundreds of packaged systems to choose from; however, if you want to do real-time data acquisition the choices are very limited and confusing. The purpose of this chapter is to provide the novice computer user with the basics needed to set up a real-time data acquisition system with the common microcomputers. This chapter will cover the following issues necessary to establish a real time data acquisition and control system: Analysis of the research problem: Definition of the problem; Description of data and sampling requirements; Cost/benefit analysis. Choice of Microcomputer hardware and software: Choice of microprocessor and bus structure; Choice of operating system; Choice of layered software. Digital Data Acquisition: Parallel Data Transmission; Serial Data Transmission; Hardware and software available. Analog Data Acquisition: Description of amplitude and frequency characteristics of the input signals; Sampling theorem; Specification of the analog to digital converter; Hardware and software available; Interface to the microcomputer. Microcomputer Control: Analog output; Digital output; Closed-Loop Control. Microcomputer data acquisition and control in the 21st Century--What is in the future? High speed digital medical equipment networks; Medical decision making and artificial intelligence.

Issues With Access to Acquisition Data and Information in the Department of Defense: Doing Data Right in Weapon System Acquisition

DTIC Science & Technology

2016-04-30

Defense: Doing Data Right in Weapon System Acquisition Nancy Moore, Senior Management Scientist, RAND Megan McKernan, Defense Research Analyst...Access to Acquisition Data & Information in the Department of Defense: Doing Data Right in Weapon System Acquisition Nancy Moore, Senior Management...With Access to Acquisition Data and Information in the Department of Defense: Doing Data Right in Weapon System Acquisition Nancy Y. Moore—is a

A hybrid approach of using symmetry technique for brain tumor segmentation.

PubMed

Saddique, Mubbashar; Kazmi, Jawad Haider; Qureshi, Kalim

2014-01-01

Tumor and related abnormalities are a major cause of disability and death worldwide. Magnetic resonance imaging (MRI) is a superior modality due to its noninvasiveness and high quality images of both the soft tissues and bones. In this paper we present two hybrid segmentation techniques and their results are compared with well-recognized techniques in this area. The first technique is based on symmetry and we call it a hybrid algorithm using symmetry and active contour (HASA). In HASA, we take refection image, calculate the difference image, and then apply the active contour on the difference image to segment the tumor. To avoid unimportant segmented regions, we improve the results by proposing an enhancement in the form of the second technique, EHASA. In EHASA, we also take reflection of the original image, calculate the difference image, and then change this image into a binary image. This binary image is mapped onto the original image followed by the application of active contouring to segment the tumor region.

Stochastic optimization model for order acceptance with multiple demand classes and uncertain demand/supply

NASA Astrophysics Data System (ADS)

Yang, Wen; Fung, Richard Y. K.

2014-06-01

This article considers an order acceptance problem in a make-to-stock manufacturing system with multiple demand classes in a finite time horizon. Demands in different periods are random variables and are independent of one another, and replenishments of inventory deviate from the scheduled quantities. The objective of this work is to maximize the expected net profit over the planning horizon by deciding the fraction of the demand that is going to be fulfilled. This article presents a stochastic order acceptance optimization model and analyses the existence of the optimal promising policies. An example of a discrete problem is used to illustrate the policies by applying the dynamic programming method. In order to solve the continuous problems, a heuristic algorithm based on stochastic approximation (HASA) is developed. Finally, the computational results of a case example illustrate the effectiveness and efficiency of the HASA approach, and make the application of the proposed model readily acceptable.

Coring Sample Acquisition Tool

NASA Technical Reports Server (NTRS)

Haddad, Nicolas E.; Murray, Saben D.; Walkemeyer, Phillip E.; Badescu, Mircea; Sherrit, Stewart; Bao, Xiaoqi; Kriechbaum, Kristopher L.; Richardson, Megan; Klein, Kerry J.

2012-01-01

A sample acquisition tool (SAT) has been developed that can be used autonomously to sample drill and capture rock cores. The tool is designed to accommodate core transfer using a sample tube to the IMSAH (integrated Mars sample acquisition and handling) SHEC (sample handling, encapsulation, and containerization) without ever touching the pristine core sample in the transfer process.

Bayesian reconstruction of projection reconstruction NMR (PR-NMR).

PubMed

Yoon, Ji Won

2014-11-01

Projection reconstruction nuclear magnetic resonance (PR-NMR) is a technique for generating multidimensional NMR spectra. A small number of projections from lower-dimensional NMR spectra are used to reconstruct the multidimensional NMR spectra. In our previous work, it was shown that multidimensional NMR spectra are efficiently reconstructed using peak-by-peak based reversible jump Markov chain Monte Carlo (RJMCMC) algorithm. We propose an extended and generalized RJMCMC algorithm replacing a simple linear model with a linear mixed model to reconstruct close NMR spectra into true spectra. This statistical method generates samples in a Bayesian scheme. Our proposed algorithm is tested on a set of six projections derived from the three-dimensional 700 MHz HNCO spectrum of a protein HasA. Copyright © 2014 Elsevier Ltd. All rights reserved.

Cryptococcus neoformans Requires the ESCRT Protein Vps23 for Iron Acquisition from Heme, for Capsule Formation, and for Virulence

PubMed Central

Hu, Guanggan; Caza, Mélissa; Cadieux, Brigitte; Chan, Vivienne; Liu, Victor

2013-01-01

Iron availability is a key regulator of virulence factor elaboration in Cryptococcus neoformans, the causative agent of fungal meningoencephalitis in HIV/AIDS patients. In addition, iron is an essential nutrient for pathogen proliferation in mammalian hosts but little is known about the mechanisms of iron sensing and uptake in fungal pathogens that attack humans. In this study, we mutagenized C. neoformans by Agrobacterium-mediated T-DNA insertion and screened for mutants with reduced growth on heme as the sole iron source. Among 34 mutants, we identified a subset with insertions in the gene for the ESCRT-I (endosomal sorting complex required for transport) protein Vps23 that resulted in a growth defect on heme, presumably due to a defect in uptake via endocytosis or misregulation of iron acquisition from heme. Remarkably, vps23 mutants were also defective in the elaboration of the cell-associated capsular polysaccharide that is a major virulence factor, while overexpression of Vps23 resulted in cells with a slightly enlarged capsule. These phenotypes were mirrored by a virulence defect in the vps23 mutant in a mouse model of cryptococcosis and by hypervirulence of the overexpression strain. Overall, these results reveal an important role for trafficking via ESCRT functions in both heme uptake and capsule formation, and they further reinforce the connection between iron and virulence factor deployment in C. neoformans. PMID:23132495

Cryptococcus neoformans requires the ESCRT protein Vps23 for iron acquisition from heme, for capsule formation, and for virulence.

PubMed

Hu, Guanggan; Caza, Mélissa; Cadieux, Brigitte; Chan, Vivienne; Liu, Victor; Kronstad, James

2013-01-01

Iron availability is a key regulator of virulence factor elaboration in Cryptococcus neoformans, the causative agent of fungal meningoencephalitis in HIV/AIDS patients. In addition, iron is an essential nutrient for pathogen proliferation in mammalian hosts but little is known about the mechanisms of iron sensing and uptake in fungal pathogens that attack humans. In this study, we mutagenized C. neoformans by Agrobacterium-mediated T-DNA insertion and screened for mutants with reduced growth on heme as the sole iron source. Among 34 mutants, we identified a subset with insertions in the gene for the ESCRT-I (endosomal sorting complex required for transport) protein Vps23 that resulted in a growth defect on heme, presumably due to a defect in uptake via endocytosis or misregulation of iron acquisition from heme. Remarkably, vps23 mutants were also defective in the elaboration of the cell-associated capsular polysaccharide that is a major virulence factor, while overexpression of Vps23 resulted in cells with a slightly enlarged capsule. These phenotypes were mirrored by a virulence defect in the vps23 mutant in a mouse model of cryptococcosis and by hypervirulence of the overexpression strain. Overall, these results reveal an important role for trafficking via ESCRT functions in both heme uptake and capsule formation, and they further reinforce the connection between iron and virulence factor deployment in C. neoformans.

Automated Acquisition Systems: Keynote Address.

ERIC Educational Resources Information Center

Boss, Richard D.

1980-01-01

The 1980s offer libraries numerous automated acquisitions alternatives, including turnkey systems from circulation system vendors and the acquisition subsystems of the bibliographic utilities. Integration of systems from several sources poses the principal problem. (Author/RAA)

Studying Protein-Protein Interactions by Biotin AP-Tagged Pulldown and LTQ-Orbitrap Mass Spectrometry.

PubMed

Xie, Zhongqiu; Jia, Yuemeng; Li, Hui

2017-01-01

The study of protein-protein interactions represents a key aspect of biological research. Identifying unknown protein binding partners using mass spectrometry (MS)-based proteomics has evolved into an indispensable strategy in drug discovery. The classic approach of immunoprecipitation with specific antibodies against the proteins of interest has limitations, such as the need for immunoprecipitation-qualified antibody. The biotin AP-tag pull-down system has the advantage of high specificity, ease of use, and no requirement for antibody. It is based on the high specificity, high affinity interaction between biotin and streptavidin. After pulldown, in-gel tryptic digestion and tandem mass spectrometry (MS/MS) analysis of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) protein bands can be performed. In this work, we provide protocols that can be used for the identification of proteins that interact with FOXM1, a protein that has recently emerged as a potential biomarker and drug target in oncotherapy, as an example. We focus on the pull-down procedure and assess the efficacy of the pulldown with known FOXM1 interactors such as β-catenin. We use a high performance LTQ Orbitrap MSn system that combines rapid LTQ ion trap data acquisition with high mass accuracy Orbitrap analysis to identify the interacting proteins.

THE SAGA OF A MALE FERTILITY PROTEIN (SP22)

EPA Science Inventory

Toxicologic studies designed to identify chemical-induced alterations in the structure and function of the epididymis, particularly the acquisition of fertility by proximal cauda epididymal sperm, have lead to the discovery of a novel sperm protein (SP22) that is well correlated ...

A Pseudomonas T6SS effector recruits PQS-containing outer membrane vesicles for iron acquisition

PubMed Central

Lin, Jinshui; Zhang, Weipeng; Cheng, Juanli; Yang, Xu; Zhu, Kaixiang; Wang, Yao; Wei, Gehong; Qian, Pei-Yuan; Luo, Zhao-Qing; Shen, Xihui

2017-01-01

Iron sequestration by host proteins contributes to the defence against bacterial pathogens, which need iron for their metabolism and virulence. A Pseudomonas aeruginosa mutant lacking all three known iron acquisition systems retains the ability to grow in media containing iron chelators, suggesting the presence of additional pathways involved in iron uptake. Here we screen P. aeruginosa mutants defective in growth in iron-depleted media and find that gene PA2374, proximal to the type VI secretion system H3 (H3-T6SS), functions synergistically with known iron acquisition systems. PA2374 (which we have renamed TseF) appears to be secreted by H3-T6SS and is incorporated into outer membrane vesicles (OMVs) by directly interacting with the iron-binding Pseudomonas quinolone signal (PQS), a cell–cell signalling compound. TseF facilitates the delivery of OMV-associated iron to bacterial cells by engaging the Fe(III)-pyochelin receptor FptA and the porin OprF. Our results reveal links between type VI secretion, cell–cell signalling and classic siderophore receptors for iron acquisition in P. aeruginosa. PMID:28348410

PPE Surface Proteins Are Required for Heme Utilization by Mycobacterium tuberculosis

PubMed Central

Mitra, Avishek; Speer, Alexander; Lin, Kan; Ehrt, Sabine

2017-01-01

ABSTRACT Iron is essential for replication of Mycobacterium tuberculosis, but iron is efficiently sequestered in the human host during infection. Heme constitutes the largest iron reservoir in the human body and is utilized by many bacterial pathogens as an iron source. While heme acquisition is well studied in other bacterial pathogens, little is known in M. tuberculosis. To identify proteins involved in heme utilization by M. tuberculosis, a transposon mutant library was screened for resistance to the toxic heme analog gallium(III)-porphyrin (Ga-PIX). Inactivation of the ppe36, ppe62, and rv0265c genes resulted in resistance to Ga-PIX. Growth experiments using isogenic M. tuberculosis deletion mutants showed that PPE36 is essential for heme utilization by M. tuberculosis, while the functions of PPE62 and Rv0265c are partially redundant. None of the genes restored growth of the heterologous M. tuberculosis mutants, indicating that the proteins encoded by the genes have separate functions. PPE36, PPE62, and Rv0265c bind heme as shown by surface plasmon resonance spectroscopy and are associated with membranes. Both PPE36 and PPE62 proteins are cell surface accessible, while the Rv0265c protein is probably located in the periplasm. PPE36 and PPE62 are, to our knowledge, the first proline-proline-glutamate (PPE) proteins of M. tuberculosis that bind small molecules and are involved in nutrient acquisition. The absence of a virulence defect of the ppe36 deletion mutant indicates that the different iron acquisition pathways of M. tuberculosis may substitute for each other during growth and persistence in mice. The emerging model of heme utilization by M. tuberculosis as derived from this study is substantially different from those of other bacteria. PMID:28119467

Protein turnover measurement using selected reaction monitoring-mass spectrometry (SRM-MS)

PubMed Central

Holman, Stephen W.; Hammond, Dean E.; Simpson, Deborah M.; Waters, John; Hurst, Jane L.

2016-01-01

Protein turnover represents an important mechanism in the functioning of cells, with deregulated synthesis and degradation of proteins implicated in many diseased states. Therefore, proteomics strategies to measure turnover rates with high confidence are of vital importance to understanding many biological processes. In this study, the more widely used approach of non-targeted precursor ion signal intensity (MS1) quantification is compared with selected reaction monitoring (SRM), a data acquisition strategy that records data for specific peptides, to determine if improved quantitative data would be obtained using a targeted quantification approach. Using mouse liver as a model system, turnover measurement of four tricarboxylic acid cycle proteins was performed using both MS1 and SRM quantification strategies. SRM outperformed MS1 in terms of sensitivity and selectivity of measurement, allowing more confident determination of protein turnover rates. SRM data are acquired using cheaper and more widely available tandem quadrupole mass spectrometers, making the approach accessible to a larger number of researchers than MS1 quantification, which is best performed on high mass resolution instruments. SRM acquisition is ideally suited to focused studies where the turnover of tens of proteins is measured, making it applicable in determining the dynamics of proteins complexes and complete metabolic pathways. This article is part of the themed issue ‘Quantitative mass spectrometry’. PMID:27644981

Improved Quantitative Plant Proteomics via the Combination of Targeted and Untargeted Data Acquisition

PubMed Central

Hart-Smith, Gene; Reis, Rodrigo S.; Waterhouse, Peter M.; Wilkins, Marc R.

2017-01-01

Quantitative proteomics strategies – which are playing important roles in the expanding field of plant molecular systems biology – are traditionally designated as either hypothesis driven or non-hypothesis driven. Many of these strategies aim to select individual peptide ions for tandem mass spectrometry (MS/MS), and to do this mixed hypothesis driven and non-hypothesis driven approaches are theoretically simple to implement. In-depth investigations into the efficacies of such approaches have, however, yet to be described. In this study, using combined samples of unlabeled and metabolically 15N-labeled Arabidopsis thaliana proteins, we investigate the mixed use of targeted data acquisition (TDA) and data dependent acquisition (DDA) – referred to as TDA/DDA – to facilitate both hypothesis driven and non-hypothesis driven quantitative data collection in individual LC-MS/MS experiments. To investigate TDA/DDA for hypothesis driven data collection, 7 miRNA target proteins of differing size and abundance were targeted using inclusion lists comprised of 1558 m/z values, using 3 different TDA/DDA experimental designs. In samples in which targeted peptide ions were of particularly low abundance (i.e., predominantly only marginally above mass analyser detection limits), TDA/DDA produced statistically significant increases in the number of targeted peptides identified (230 ± 8 versus 80 ± 3 for DDA; p = 1.1 × 10-3) and quantified (35 ± 3 versus 21 ± 2 for DDA; p = 0.038) per experiment relative to the use of DDA only. These expected improvements in hypothesis driven data collection were observed alongside unexpected improvements in non-hypothesis driven data collection. Untargeted peptide ions with m/z values matching those in inclusion lists were repeatedly identified and quantified across technical replicate TDA/DDA experiments, resulting in significant increases in the percentages of proteins repeatedly quantified in TDA/DDA experiments only relative to DDA

78 FR 13544 - Defense Federal Acquisition Regulation Supplement: Acquisition of Tents and Other Temporary...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-28

... acquisition of domestic tents to include the structural components of tents, applicable to acquisitions that... rule. A. Tents and Structural Components of Tents Question: Does the term ``structural components... 225.7002-1(a)(3) and 252.225-7012(b)(3) ensures clarity that the phrase ``structural components'' only...

48 CFR Appendix - Federal Acquisition Regulation (FAR) Index

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Federal Acquisition Regulation (FAR) Index Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT USE OF GOVERNMENT SOURCES BY CONTRACTORS Contractor Use of Interagency Fleet Management System...

Evolution of a protein folding nucleus.

PubMed

Xia, Xue; Longo, Liam M; Sutherland, Mason A; Blaber, Michael

2016-07-01

The folding nucleus (FN) is a cryptic element within protein primary structure that enables an efficient folding pathway and is the postulated heritable element in the evolution of protein architecture; however, almost nothing is known regarding how the FN structurally changes as complex protein architecture evolves from simpler peptide motifs. We report characterization of the FN of a designed purely symmetric β-trefoil protein by ϕ-value analysis. We compare the structure and folding properties of key foldable intermediates along the evolutionary trajectory of the β-trefoil. The results show structural acquisition of the FN during gene fusion events, incorporating novel turn structure created by gene fusion. Furthermore, the FN is adjusted by circular permutation in response to destabilizing functional mutation. FN plasticity by way of circular permutation is made possible by the intrinsic C3 cyclic symmetry of the β-trefoil architecture, identifying a possible selective advantage that helps explain the prevalence of cyclic structural symmetry in the proteome. © 2015 The Protein Society.

Learning the manifold of quality ultrasound acquisition.

PubMed

El-Zehiry, Noha; Yan, Michelle; Good, Sara; Fang, Tong; Zhou, S Kevin; Grady, Leo

2013-01-01

Ultrasound acquisition is a challenging task that requires simultaneous adjustment of several acquisition parameters (the depth, the focus, the frequency and its operation mode). If the acquisition parameters are not properly chosen, the resulting image will have a poor quality and will degrade the patient diagnosis and treatment workflow. Several hardware-based systems for autotuning the acquisition parameters have been previously proposed, but these solutions were largely abandoned because they failed to properly account for tissue inhomogeneity and other patient-specific characteristics. Consequently, in routine practice the clinician either uses population-based parameter presets or manually adjusts the acquisition parameters for each patient during the scan. In this paper, we revisit the problem of autotuning the acquisition parameters by taking a completely novel approach and producing a solution based on image analytics. Our solution is inspired by the autofocus capability of conventional digital cameras, but is significantly more challenging because the number of acquisition parameters is large and the determination of "good quality" images is more difficult to assess. Surprisingly, we show that the set of acquisition parameters which produce images that are favored by clinicians comprise a 1D manifold, allowing for a real-time optimization to maximize image quality. We demonstrate our method for acquisition parameter autotuning on several live patients, showing that our system can start with a poor initial set of parameters and automatically optimize the parameters to produce high quality images.

EXPECT: Explicit Representations for Flexible Acquisition

NASA Technical Reports Server (NTRS)

Swartout, BIll; Gil, Yolanda

1995-01-01

To create more powerful knowledge acquisition systems, we not only need better acquisition tools, but we need to change the architecture of the knowledge based systems we create so that their structure will provide better support for acquisition. Current acquisition tools permit users to modify factual knowledge but they provide limited support for modifying problem solving knowledge. In this paper, the authors argue that this limitation (and others) stem from the use of incomplete models of problem-solving knowledge and inflexible specification of the interdependencies between problem-solving and factual knowledge. We describe the EXPECT architecture which addresses these problems by providing an explicit representation for problem-solving knowledge and intent. Using this more explicit representation, EXPECT can automatically derive the interdependencies between problem-solving and factual knowledge. By deriving these interdependencies from the structure of the knowledge-based system itself EXPECT supports more flexible and powerful knowledge acquisition.

48 CFR 5.405 - Exchange of acquisition information.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Exchange of acquisition... ACQUISITION PLANNING PUBLICIZING CONTRACT ACTIONS Release of Information 5.405 Exchange of acquisition... than one contracting activity within an agency, the exchange and coordination of pertinent information...

48 CFR 3032.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Simplified acquisition procedures financing. 3032.003 Section 3032.003 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND... FINANCING Scope of Part 3032.003 Simplified acquisition procedures financing. Contract financing may be...

Schizophrenia and second language acquisition.

PubMed

Bersudsky, Yuly; Fine, Jonathan; Gorjaltsan, Igor; Chen, Osnat; Walters, Joel

2005-05-01

Language acquisition involves brain processes that can be affected by lesions or dysfunctions in several brain systems and second language acquisition may depend on different brain substrates than first language acquisition in childhood. A total of 16 Russian immigrants to Israel, 8 diagnosed schizophrenics and 8 healthy immigrants, were compared. The primary data for this study were collected via sociolinguistic interviews. The two groups use language and learn language in very much the same way. Only exophoric reference and blocking revealed meaningful differences between the schizophrenics and healthy counterparts. This does not mean of course that schizophrenia does not induce language abnormalities. Our study focuses on those aspects of language that are typically difficult to acquire in second language acquisition. Despite the cognitive compromises in schizophrenia and the manifest atypicalities in language of speakers with schizophrenia, the process of acquiring a second language seems relatively unaffected by schizophrenia.

STIS Target Acquisitions During SMOV

NASA Astrophysics Data System (ADS)

Katsanis, Rocio M.; Downes, Ron; Hartig, George; Kraemer, Steve

1997-07-01

We summarize the first results on the analysis of in-flight STIS target acquisition (ACQ and ACQ/PEAK). These results show that the STIS target acquisition (ACQ) is working very accurately for point sources (within 0.5 pixels = 0.025 arcseconds), about 4 times better than specified in the Instrument Handbook. As a result of the accuracy of the ACQ algorithm, we are no longer recommending to perform ACQ/PEAKs for the 0.2 arcsecond wide slits. For diffuse acquisitions the accuracy varies with target size. Although analysis of ACQ/PEAK data is hampered by a flight software problem, we anticipate that peakups will be accurate to roughly ±5% of the slit width (instead of the ±15% pr eviously advertised). We are implementing several enhancements to the flight software that will take effect by mid- August to improve the quality of the acquisitions.

26 CFR 1.279-3 - Corporate acquisition indebtedness.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Corporate acquisition indebtedness. 1.279-3... TAX (CONTINUED) INCOME TAXES Items Not Deductible § 1.279-3 Corporate acquisition indebtedness. (a) Corporate acquisition indebtedness. For purposes of section 279, the term corporate acquisition indebtedness...

48 CFR 218.170 - Additional acquisition flexibilities.

Code of Federal Regulations, 2012 CFR

2012-10-01

... flexibilities. 218.170 Section 218.170 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES EMERGENCY ACQUISITIONS Available... circumstances. See PGI 206.302-2. (b) Use of advance Military Interdepartmental Purchase Request (MIPR). For...

48 CFR 218.170 - Additional acquisition flexibilities.

Code of Federal Regulations, 2013 CFR

2013-10-01

... flexibilities. 218.170 Section 218.170 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES EMERGENCY ACQUISITIONS Available... circumstances. See PGI 206.302-2. (b) Use of advance Military Interdepartmental Purchase Request (MIPR). For...

48 CFR 218.170 - Additional acquisition flexibilities.

Code of Federal Regulations, 2014 CFR

2014-10-01

... flexibilities. 218.170 Section 218.170 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES EMERGENCY ACQUISITIONS Available... circumstances. See PGI 206.302-2. (b) Use of advance Military Interdepartmental Purchase Request (MIPR). For...

48 CFR 218.170 - Additional acquisition flexibilities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... flexibilities. 218.170 Section 218.170 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES EMERGENCY ACQUISITIONS Available... circumstances. See PGI 206.302-2. (b) Use of advance Military Interdepartmental Purchase Request (MIPR). For...

PECAN: library-free peptide detection for data-independent acquisition tandem mass spectrometry data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ting, Ying S.; Egertson, Jarrett D.; Bollinger, James G.

Data-independent acquisition (DIA) is an emerging mass spectrometry (MS)-based technique for unbiased and reproducible measurement of protein mixtures. DIA tandem mass spectrometry spectra are often highly multiplexed, containing product ions from multiple cofragmenting precursors. Detecting peptides directly from DIA data is therefore challenging; most DIA data analyses require spectral libraries. Here we present PECECAN (http://pecan.maccosslab.org), a library-free, peptide-centric tool that robustly and accurately detects peptides directly from DIA data. PECECAN reports evidence of detection based on product ion scoring, which enables detection of low-abundance analytes with poor precursor ion signal. We demonstrate the chromatographic peak picking accuracy and peptide detectionmore » capability of PECECAN, and we further validate its detection with data-dependent acquisition and targeted analyses. Lastly, we used PECECAN to build a plasma proteome library from DIA data and to query known sequence variants.« less

Pseudotargeted MS Method for the Sensitive Analysis of Protein Phosphorylation in Protein Complexes.

PubMed

Lyu, Jiawen; Wang, Yan; Mao, Jiawei; Yao, Yating; Wang, Shujuan; Zheng, Yong; Ye, Mingliang

2018-05-15

In this study, we presented an enrichment-free approach for the sensitive analysis of protein phosphorylation in minute amounts of samples, such as purified protein complexes. This method takes advantage of the high sensitivity of parallel reaction monitoring (PRM). Specifically, low confident phosphopeptides identified from the data-dependent acquisition (DDA) data set were used to build a pseudotargeted list for PRM analysis to allow the identification of additional phosphopeptides with high confidence. The development of this targeted approach is very easy as the same sample and the same LC-system were used for the discovery and the targeted analysis phases. No sample fractionation or enrichment was required for the discovery phase which allowed this method to analyze minute amount of sample. We applied this pseudotargeted MS method to quantitatively examine phosphopeptides in affinity purified endogenous Shc1 protein complexes at four temporal stages of EGF signaling and identified 82 phospho-sites. To our knowledge, this is the highest number of phospho-sites identified from the protein complexes. This pseudotargeted MS method is highly sensitive in the identification of low abundance phosphopeptides and could be a powerful tool to study phosphorylation-regulated assembly of protein complex.

48 CFR Appendix - Federal Acquisition Regulation (FAR) Index

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Federal Acquisition Regulation (FAR) Index Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT USE OF GOVERNMENT SOURCES BY CONTRACTORS Contractor Use of Interagency Fleet Management System (IFMS) Contract clause. FAR Index Federal...

48 CFR Appendix - Federal Acquisition Regulation (FAR) Index

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Federal Acquisition Regulation (FAR) Index Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT USE OF GOVERNMENT SOURCES BY CONTRACTORS Contractor Use of Interagency Fleet Management System (IFMS) Contract clause. FAR Index Federal...

48 CFR 1519.501 - Review of acquisitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Review of acquisitions. 1519.501 Section 1519.501 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY... (SBS) shall initiate recommendations to the Contracting Officer for small business set-asides with...

77 FR 30365 - Defense Federal Acquisition Regulation Supplement: Report on Waiver of Prohibition on Acquisition...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-22

... Chinese Military Companies (DFARS Case 2012-D023) AGENCY: Defense Acquisition Regulations System... of the prohibition on acquisition of United States Munitions List items from Communist Chinese... List items from Communist Chinese military companies. The prior requirement was for a report within 30...

32 CFR 644.102 - Examples of involuntary acquisitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 4 2010-07-01 2010-07-01 true Examples of involuntary acquisitions. 644.102... PROPERTY REAL ESTATE HANDBOOK Acquisition Involuntary Acquisition by the United States § 644.102 Examples... property, as prescribed by Pub. L. 91-646. Examples of involuntary acquisition are: (a) Damage to real...

Business Acquisition and Implementation

DTIC Science & Technology

2006-01-01

I am proposing that this technical paper be broken down into two phases. Phase one will consist of business and land acquisition. Phase two will...consist of business implementation. The business acquisition phase will document the process of forming a limited liability company (LLC) from a sole...proprietorship with the intention of real estate development and personal asset protection. I do not require a business license but I will need to

48 CFR 18.113 - Interagency acquisitions under the Economy Act.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Interagency acquisitions under the Economy Act. 18.113 Section 18.113 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACTING METHODS AND CONTRACT TYPES EMERGENCY ACQUISITIONS Available Acquisition Flexibilities 18.113 Interagency acquisitions under...

75 FR 10567 - Commerce Acquisition Regulation (CAR)

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-08

... acquisitions, contract financing, protests, disputes, and appeals, major system acquisitions, research and... appeals. SUBCHAPTER F--SPECIAL CATEGORIES OF CONTRACTING 1334 Major system acquisition. 1335 Research and....603-2, selection of contracting officers shall be based upon Section 4 of CAM 1301.6. 1301.603-3...

23 CFR 710.309 - Acquisition.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 23 Highways 1 2012-04-01 2012-04-01 false Acquisition. 710.309 Section 710.309 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT RIGHT-OF-WAY AND REAL ESTATE Project Development § 710.309 Acquisition. The process of acquiring real property includes...

Acquisition Policies. SPEC Kit 12.

ERIC Educational Resources Information Center

Association of Research Libraries, Washington, DC. Office of Management Studies.

This collection of acquisition materials from member libraries of the Association of Research Libraries (ARL) contains: (1) acquisition policies from Brigham Young University, Iowa State University, and the University of Cincinnati; (2) book selection policies from the University of Nebraska Undergraduate Library and Georgetown University; (3) an…

75 FR 39420 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-44; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-08

... Federal Acquisition Circular (FAC) 2005-44 which amends the Federal Acquisition Regulation (FAR). Interested parties may obtain further information regarding this rule by referring to FAC 2005-44 which... . FOR FURTHER INFORMATION CONTACT: The analyst whose name appears in the table below. Please cite FAC...

48 CFR 1480.102 - Buy Indian Act acquisition regulations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Buy Indian Act acquisition... INDIAN AFFAIRS SUPPLEMENT ACQUISITIONS UNDER THE BUY INDIAN ACT Subpart 1480.1 General 1480.102 Buy Indian Act acquisition regulations. (a) This part supplements Federal Acquisition Regulation (FAR) and...

Acoustic window planning for ultrasound acquisition.

PubMed

Göbl, Rüdiger; Virga, Salvatore; Rackerseder, Julia; Frisch, Benjamin; Navab, Nassir; Hennersperger, Christoph

2017-06-01

Autonomous robotic ultrasound has recently gained considerable interest, especially for collaborative applications. Existing methods for acquisition trajectory planning are solely based on geometrical considerations, such as the pose of the transducer with respect to the patient surface. This work aims at establishing acoustic window planning to enable autonomous ultrasound acquisitions of anatomies with restricted acoustic windows, such as the liver or the heart. We propose a fully automatic approach for the planning of acquisition trajectories, which only requires information about the target region as well as existing tomographic imaging data, such as X-ray computed tomography. The framework integrates both geometrical and physics-based constraints to estimate the best ultrasound acquisition trajectories with respect to the available acoustic windows. We evaluate the developed method using virtual planning scenarios based on real patient data as well as for real robotic ultrasound acquisitions on a tissue-mimicking phantom. The proposed method yields superior image quality in comparison with a naive planning approach, while maintaining the necessary coverage of the target. We demonstrate that by taking image formation properties into account acquisition planning methods can outperform naive plannings. Furthermore, we show the need for such planning techniques, since naive approaches are not sufficient as they do not take the expected image quality into account.

The differential role of cortical protein synthesis in taste memory formation and persistence

NASA Astrophysics Data System (ADS)

Levitan, David; Gal-Ben-Ari, Shunit; Heise, Christopher; Rosenberg, Tali; Elkobi, Alina; Inberg, Sharon; Sala, Carlo; Rosenblum, Kobi

2016-05-01

The current dogma suggests that the formation of long-term memory (LTM) is dependent on protein synthesis but persistence of the memory trace is not. However, many of the studies examining the effect of protein synthesis inhibitors (PSIs) on LTM persistence were performed in the hippocampus, which is known to have a time-dependent role in memory storage, rather than the cortex, which is considered to be the main structure to store long-term memories. Here we studied the effect of PSIs on LTM formation and persistence in male Wistar Hola (n⩾5) rats by infusing the protein synthesis inhibitor, anisomycin (100 μg, 1 μl), into the gustatory cortex (GC) during LTM formation and persistence in conditioned taste aversion (CTA). We found that local anisomycin infusion to the GC before memory acquisition impaired LTM formation (P=8.9E-5), but had no effect on LTM persistence when infused 3 days post acquisition (P=0.94). However, when we extended the time interval between treatment with anisomycin and testing from 3 days to 14 days, LTM persistence was enhanced (P=0.01). The enhancement was on the background of stable and non-declining memory, and was not recapitulated by another amnesic agent, APV (10 μg, 1 μl), an N-methyl-D-aspartate receptor antagonist (P=0.54). In conclusion, CTA LTM remains sensitive to the action of PSIs in the GC even 3 days following memory acquisition. This sensitivity is differentially expressed between the formation and persistence of LTM, suggesting that increased cortical protein synthesis promotes LTM formation, whereas decreased protein synthesis promotes LTM persistence.

Centaur propellant acquisition system

NASA Technical Reports Server (NTRS)

Blatt, M. H.; Aydelott, J. C.

1975-01-01

The desirability of replacing the hydrogen peroxide settling system of the Centaur D-1S with a capillary acquisition system was evaluated. A comprehensive screening was performed to select the most promising capillary device fluid acquisition, thermal conditioning, and fabrication techniques. Refillable start baskets and bypass feed start tanks were selected for detailed design. Critical analysis areas were settling and refilling, start sequence development with an initially dry boost pump, and cooling the fluid delivered to the boost pump to provide the necessary net positive suction head (NPSH). Design drawings were prepared for start basket and start tank concepts for both the liquid oxygen and liquid hydrogen tanks. System comparisons indicated that the start baskets using wicking flow for thermal conditioning, and thermal subcooling for providing boost pump NPSH, are the most desirable systems for future Centaur acquisition system development.

Developing capital investment guidelines for acquisitions.

PubMed

Bergman, J T; Gordon, D C

1988-05-01

Financial managers are now being asked to evaluate new investment opportunities, such as acquisitions. To do a thorough evaluation, however, it is important to have a plan of action to follow. Strategies such as establishing a framework for evaluating acquisitions based on strategic compatibility with the existing organization, establishing suitable risk-adjusted discount rates for assessing investments, and selecting the proper type of capital allocation method are all necessary steps to ensure a successful acquisition.

Improved method for identification of low abundance proteins using 2D-gel electrophoresis, MALDI-TOF and TOF/TOF

EPA Science Inventory

Introduction: Differential protein expression studies have been routinely performed in our laboratory to determine the health effects of environmentally-important chemicals. In this abstract, improvements in the in-gel protein digestion, MALDI plate spotting and data acquisition...

48 CFR 207.401 - Acquisition considerations.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF DEFENSE ACQUISITION PLANNING ACQUISITION PLANNING Equipment Lease or Purchase 207.401... prepare and provide the contracting officer with the justification supporting the decision to lease or purchase. ...

7 CFR 982.16 - Inshell trade acquisitions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 8 2011-01-01 2011-01-01 false Inshell trade acquisitions. 982.16 Section 982.16... WASHINGTON Order Regulating Handling Definitions § 982.16 Inshell trade acquisitions. Inshell trade acquisitions means the quantity of inshell hazelnuts acquired by the trade from all handlers during a marketing...

7 CFR 982.16 - Inshell trade acquisitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Inshell trade acquisitions. 982.16 Section 982.16... WASHINGTON Order Regulating Handling Definitions § 982.16 Inshell trade acquisitions. Inshell trade acquisitions means the quantity of inshell hazelnuts acquired by the trade from all handlers during a marketing...

7 CFR 982.16 - Inshell trade acquisitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 8 2014-01-01 2014-01-01 false Inshell trade acquisitions. 982.16 Section 982.16... WASHINGTON Order Regulating Handling Definitions § 982.16 Inshell trade acquisitions. Inshell trade acquisitions means the quantity of inshell hazelnuts acquired by the trade from all handlers during a marketing...

7 CFR 982.16 - Inshell trade acquisitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 8 2013-01-01 2013-01-01 false Inshell trade acquisitions. 982.16 Section 982.16... WASHINGTON Order Regulating Handling Definitions § 982.16 Inshell trade acquisitions. Inshell trade acquisitions means the quantity of inshell hazelnuts acquired by the trade from all handlers during a marketing...

The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition.

PubMed

Si, Meiru; Wang, Yao; Zhang, Bing; Zhao, Chao; Kang, Yiwen; Bai, Haonan; Wei, Dawei; Zhu, Lingfang; Zhang, Lei; Dong, Tao G; Shen, Xihui

2017-07-25

The type VI secretion system was recently reported to be involved in zinc acquisition, but the underlying mechanism remains unclear. Here, we report that Burkholderia thailandensis T6SS4 is involved in zinc acquisition via secretion of a zinc-scavenging protein, TseZ, that interacts with the outer membrane heme transporter HmuR. We find that HmuR is a redox-regulated dual-functional transporter that transports heme iron under normal conditions but zinc upon sensing extracellular oxidative stress, triggered by formation of an intramolecular disulfide bond. Acting as the first line of defense against oxidative stress, HmuR not only guarantees an immediate response to the changing environment but also provides a fine-tuned mechanism that allows a gradual response to perceived stress. The T6SS/HmuR-mediated active zinc transport system is also involved in bacterial virulence and contact-independent bacterial competition. We describe a sophisticated bacterial zinc acquisition mechanism affording insights into the role of metal ion transport systems. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

O Antigen Modulates Insect Vector Acquisition of the Bacterial Plant Pathogen Xylella fastidiosa

PubMed Central

Rapicavoli, Jeannette N.; Kinsinger, Nichola; Perring, Thomas M.; Backus, Elaine A.; Shugart, Holly J.; Walker, Sharon

2015-01-01

Hemipteran insect vectors transmit the majority of plant pathogens. Acquisition of pathogenic bacteria by these piercing/sucking insects requires intimate associations between the bacterial cells and insect surfaces. Lipopolysaccharide (LPS) is the predominant macromolecule displayed on the cell surface of Gram-negative bacteria and thus mediates bacterial interactions with the environment and potential hosts. We hypothesized that bacterial cell surface properties mediated by LPS would be important in modulating vector-pathogen interactions required for acquisition of the bacterial plant pathogen Xylella fastidiosa, the causative agent of Pierce's disease of grapevines. Utilizing a mutant that produces truncated O antigen (the terminal portion of the LPS molecule), we present results that link this LPS structural alteration to a significant decrease in the attachment of X. fastidiosa to blue-green sharpshooter foreguts. Scanning electron microscopy confirmed that this defect in initial attachment compromised subsequent biofilm formation within vector foreguts, thus impairing pathogen acquisition. We also establish a relationship between O antigen truncation and significant changes in the physiochemical properties of the cell, which in turn affect the dynamics of X. fastidiosa adhesion to the vector foregut. Lastly, we couple measurements of the physiochemical properties of the cell with hydrodynamic fluid shear rates to produce a Comsol model that predicts primary areas of bacterial colonization within blue-green sharpshooter foreguts, and we present experimental data that support the model. These results demonstrate that, in addition to reported protein adhesin-ligand interactions, O antigen is crucial for vector-pathogen interactions, specifically in the acquisition of this destructive agricultural pathogen. PMID:26386068

Diatom centromeres suggest a mechanism for nuclear DNA acquisition

DOE PAGES

Diner, Rachel E.; Noddings, Chari M.; Lian, Nathan C.; ...

2017-07-18

Centromeres are essential for cell division and growth in all eukaryotes, and knowledge of their sequence and structure guides the development of artificial chromosomes for functional cellular biology studies. Centromeric proteins are conserved among eukaryotes; however, centromeric DNA sequences are highly variable. We combined forward and reverse genetic approaches with chromatin immunoprecipitation to identify centromeres of the model diatom Phaeodactylum tricornutum. We observed 25 unique centromere sequences typically occurring once per chromosome, a finding that helps to resolve nuclear genome organization and indicates monocentric regional centromeres. Diatom centromere sequences contain low-GC content regions but lack repeats or other conserved sequencemore » features. Native and foreign sequences with similar GC content to P. tricornutum centromeres can maintain episomes and recruit the diatom centromeric histone protein CENH3, suggesting nonnative sequences can also function as diatom centromeres. Thus, simple sequence requirements may enable DNA from foreign sources to persist in the nucleus as extrachromosomal episomes, revealing a potential mechanism for organellar and foreign DNA acquisition.« less

Diatom centromeres suggest a mechanism for nuclear DNA acquisition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Diner, Rachel E.; Noddings, Chari M.; Lian, Nathan C.

Centromeres are essential for cell division and growth in all eukaryotes, and knowledge of their sequence and structure guides the development of artificial chromosomes for functional cellular biology studies. Centromeric proteins are conserved among eukaryotes; however, centromeric DNA sequences are highly variable. We combined forward and reverse genetic approaches with chromatin immunoprecipitation to identify centromeres of the model diatom Phaeodactylum tricornutum. We observed 25 unique centromere sequences typically occurring once per chromosome, a finding that helps to resolve nuclear genome organization and indicates monocentric regional centromeres. Diatom centromere sequences contain low-GC content regions but lack repeats or other conserved sequencemore » features. Native and foreign sequences with similar GC content to P. tricornutum centromeres can maintain episomes and recruit the diatom centromeric histone protein CENH3, suggesting nonnative sequences can also function as diatom centromeres. Thus, simple sequence requirements may enable DNA from foreign sources to persist in the nucleus as extrachromosomal episomes, revealing a potential mechanism for organellar and foreign DNA acquisition.« less

Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated ‘Omics Approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lindemann, Stephen R.; Mobberley, Jennifer M.; Cole, Jessica K.

The principles governing acquisition and interspecies exchange of nutrients in microbial communities and how those exchanges impact community productivity are poorly understood. Here, we examine energy and macronutrient acquisition in unicyanobacterial consortia for which species-resolved genome information exists for all members, allowing us to use multi-omic approaches to predict species’ abilities to acquire resources and examine expression of resource-acquisition genes during succession. Metabolic reconstruction indicated that a majority of heterotrophic community members lacked the genes required to directly acquire the inorganic nutrients provided in culture medium, suggesting high metabolic interdependency. The sole primary producer in consortium UCC-O, cyanobacterium Phormidium sp.more » OSCR, displayed declining expression of energy harvest, carbon fixation, and nitrate and sulfate reduction proteins but sharply increasing phosphate transporter expression over 28 days. Most heterotrophic members likewise exhibited signs of phosphorus starvation during succession. Though similar in their responses to phosphorus limitation, heterotrophs displayed species-specific expression of nitrogen acquisition genes. These results suggest niche partitioning around nitrogen sources may structure the community when organisms directly compete for limited phosphate. Such niche complementarity around nitrogen sources may increase community diversity and productivity in phosphate-limited phototrophic communities.« less

Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated ‘Omics Approach

PubMed Central

Lindemann, Stephen R.; Mobberley, Jennifer M.; Cole, Jessica K.; Markillie, L. M.; Taylor, Ronald C.; Huang, Eric; Chrisler, William B.; Wiley, H. S.; Lipton, Mary S.; Nelson, William C.; Fredrickson, James K.; Romine, Margaret F.

2017-01-01

The principles governing acquisition and interspecies exchange of nutrients in microbial communities and how those exchanges impact community productivity are poorly understood. Here, we examine energy and macronutrient acquisition in unicyanobacterial consortia for which species-resolved genome information exists for all members, allowing us to use multi-omic approaches to predict species’ abilities to acquire resources and examine expression of resource-acquisition genes during succession. Metabolic reconstruction indicated that a majority of heterotrophic community members lacked the genes required to directly acquire the inorganic nutrients provided in culture medium, suggesting high metabolic interdependency. The sole primary producer in consortium UCC-O, cyanobacterium Phormidium sp. OSCR, displayed declining expression of energy harvest, carbon fixation, and nitrate and sulfate reduction proteins but sharply increasing phosphate transporter expression over 28 days. Most heterotrophic members likewise exhibited signs of phosphorus starvation during succession. Though similar in their responses to phosphorus limitation, heterotrophs displayed species-specific expression of nitrogen acquisition genes. These results suggest niche partitioning around nitrogen sources may structure the community when organisms directly compete for limited phosphate. Such niche complementarity around nitrogen sources may increase community diversity and productivity in phosphate-limited phototrophic communities. PMID:28659875

Predicting Species-Resolved Macronutrient Acquisition during Succession in a Model Phototrophic Biofilm Using an Integrated ‘Omics Approach

DOE PAGES

Lindemann, Stephen R.; Mobberley, Jennifer M.; Cole, Jessica K.; ...

2017-06-13

The principles governing acquisition and interspecies exchange of nutrients in microbial communities and how those exchanges impact community productivity are poorly understood. Here, we examine energy and macronutrient acquisition in unicyanobacterial consortia for which species-resolved genome information exists for all members, allowing us to use multi-omic approaches to predict species’ abilities to acquire resources and examine expression of resource-acquisition genes during succession. Metabolic reconstruction indicated that a majority of heterotrophic community members lacked the genes required to directly acquire the inorganic nutrients provided in culture medium, suggesting high metabolic interdependency. The sole primary producer in consortium UCC-O, cyanobacterium Phormidium sp.more » OSCR, displayed declining expression of energy harvest, carbon fixation, and nitrate and sulfate reduction proteins but sharply increasing phosphate transporter expression over 28 days. Most heterotrophic members likewise exhibited signs of phosphorus starvation during succession. Though similar in their responses to phosphorus limitation, heterotrophs displayed species-specific expression of nitrogen acquisition genes. These results suggest niche partitioning around nitrogen sources may structure the community when organisms directly compete for limited phosphate. Such niche complementarity around nitrogen sources may increase community diversity and productivity in phosphate-limited phototrophic communities.« less

Space storable propellant acquisition system

NASA Technical Reports Server (NTRS)

Tegart, J. R.; Uney, P. E.; Anderson, J. E.; Fester, D. A.

1972-01-01

Surface tension propellant acquisition concepts for an advanced spacecraft propulsion system having a 10-year mission capability were investigated. Surface tension systems were specified because they were shown to be the best propellant acquisition technique for various interplanetery spacecraft in a prior study. A variety of surface tension concepts for accomplishing propellant acquisition were formulated for the baseline space storable propulsion module and Jupiter Orbiter mission. Analyses and evaluations were then conducted on each candidate concept to assess fabricability, performance capability, and spacecraft compatibility. A comparative evaluation of the results showed the Fruhof-class of low-g surface tension systems to be preferred for these interplanetary applications.

War-gaming application for future space systems acquisition: MATLAB implementation of war-gaming acquisition models and simulation results

NASA Astrophysics Data System (ADS)

Vienhage, Paul; Barcomb, Heather; Marshall, Karel; Black, William A.; Coons, Amanda; Tran, Hien T.; Nguyen, Tien M.; Guillen, Andy T.; Yoh, James; Kizer, Justin; Rogers, Blake A.

2017-05-01

The paper describes the MATLAB (MathWorks) programs that were developed during the REU workshop1 to implement The Aerospace Corporation developed Unified Game-based Acquisition Framework and Advanced Game - based Mathematical Framework (UGAF-AGMF) and its associated War-Gaming Engine (WGE) models. Each game can be played from the perspectives of the Department of Defense Acquisition Authority (DAA) or of an individual contractor (KTR). The programs also implement Aerospace's optimum "Program and Technical Baseline (PTB) and associated acquisition" strategy that combines low Total Ownership Cost (TOC) with innovative designs while still meeting warfighter needs. The paper also describes the Bayesian Acquisition War-Gaming approach using Monte Carlo simulations, a numerical analysis technique to account for uncertainty in decision making, which simulate the PTB development and acquisition processes and will detail the procedure of the implementation and the interactions between the games.

32 CFR 644.7 - Acquisition lines.

Code of Federal Regulations, 2010 CFR

2010-07-01

... HANDBOOK Project Planning Civil Works § 644.7 Acquisition lines. (a) Tentative acquisition lines. As..., in accordance with sound real estate practices. Accordingly, fringe tracts will not be acquired until...

Syntactic Markedness and Language Acquisition.

ERIC Educational Resources Information Center

Mazurkewich, Irene

1985-01-01

Argues that Chomsky's theory of generative grammar provides a model for second language acquisition that meets the criteria of descriptive and exploratory adequacy in accounting for the language learning process. Illustrates this by presenting data on the acquisition of dative complements and dative questions in a passive context by second…

16 CFR 801.32 - Conversion and acquisition.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Conversion and acquisition. 801.32 Section... INTERPRETATIONS UNDER THE HART-SCOTT-RODINO ANTITRUST IMPROVEMENTS ACT OF 1976 COVERAGE RULES § 801.32 Conversion and acquisition. A conversion is an acquisition within the meaning of the act. Example: Assume that...

16 CFR 801.32 - Conversion and acquisition.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Conversion and acquisition. 801.32 Section... INTERPRETATIONS UNDER THE HART-SCOTT-RODINO ANTITRUST IMPROVEMENTS ACT OF 1976 COVERAGE RULES § 801.32 Conversion and acquisition. A conversion is an acquisition within the meaning of the act. Example: Assume that...

77 FR 23371 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-58; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-18

... Federal Acquisition Circular (FAC) 2005-58, which amends the Federal Acquisition Regulation (FAR). An... parties may obtain further information regarding this rule by referring to FAC 2005-58, which precedes... analyst whose name appears in the table below. Please cite FAC 2005-58 and the FAR case number. For...

78 FR 13769 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-66; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-28

... Federal Acquisition Circular (FAC) 2005-66, which amends the Federal Acquisition Regulation (FAR). An... parties may obtain further information regarding this rule by referring to FAC 2005-66, which precedes... analyst whose name appears in the table below. Please cite FAC 2005-66 and the FAR case number. For...

76 FR 4191 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-49; Small Entity Compliance Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

... Federal Acquisition Circular (FAC) 2005-49, which amend the Federal Acquisition Regulation (FAR). Interested parties may obtain further information regarding these rules by referring to FAC 2005-49, which... analyst whose name appears in the table below. Please cite FAC 2005-49 and the specific FAR case number...

Cybersecurity:The Road Ahead for Defense Acquisition

DTIC Science & Technology

2016-06-01

tion programs. A key challenge for DoD acquisition addressing the cyber threat is how do we “ bake in” cybersecurity for our DoD ac- quisition programs...cybersecurity must be “ baked in” the design of our warfighting systems. Supporting policy and best practices for effective cyberse- curity in acquisition...contacted at steve.mills@dau.mil and steve.monks@ dau.mil. A key challenge for DoD acquisition addressing the cyber threat is how do we “ bake in” cybersecurity for our DoD acquisition programs vs. “bolting it on.”

48 CFR 873.105 - Acquisition planning.

Code of Federal Regulations, 2012 CFR

2012-10-01

... procedures, the contracting officer or the acquisition team, as appropriate, must conduct market research to..., briefing materials shall be presented to address the acquisition plan topics and structure in FAR 7.105...

48 CFR 873.105 - Acquisition planning.

Code of Federal Regulations, 2011 CFR

2011-10-01

... procedures, the contracting officer or the acquisition team, as appropriate, must conduct market research to..., briefing materials shall be presented to address the acquisition plan topics and structure in FAR 7.105...

48 CFR 873.105 - Acquisition planning.

Code of Federal Regulations, 2014 CFR

2014-10-01

... procedures, the contracting officer or the acquisition team, as appropriate, must conduct market research to..., briefing materials shall be presented to address the acquisition plan topics and structure in FAR 7.105...

48 CFR 873.105 - Acquisition planning.

Code of Federal Regulations, 2013 CFR

2013-10-01

... procedures, the contracting officer or the acquisition team, as appropriate, must conduct market research to..., briefing materials shall be presented to address the acquisition plan topics and structure in FAR 7.105...

48 CFR 873.105 - Acquisition planning.

Code of Federal Regulations, 2010 CFR

2010-10-01

... procedures, the contracting officer or the acquisition team, as appropriate, must conduct market research to..., briefing materials shall be presented to address the acquisition plan topics and structure in FAR 7.105...

Dynamics of Hippocampal Protein Expression During Long-term Spatial Memory Formation*

PubMed Central

Borovok, Natalia; Nesher, Elimelech; Levin, Yishai; Reichenstein, Michal; Pinhasov, Albert

2016-01-01

Spatial memory depends on the hippocampus, which is particularly vulnerable to aging. This vulnerability has implications for the impairment of navigation capacities in older people, who may show a marked drop in performance of spatial tasks with advancing age. Contemporary understanding of long-term memory formation relies on molecular mechanisms underlying long-term synaptic plasticity. With memory acquisition, activity-dependent changes occurring in synapses initiate multiple signal transduction pathways enhancing protein turnover. This enhancement facilitates de novo synthesis of plasticity related proteins, crucial factors for establishing persistent long-term synaptic plasticity and forming memory engrams. Extensive studies have been performed to elucidate molecular mechanisms of memory traces formation; however, the identity of plasticity related proteins is still evasive. In this study, we investigated protein turnover in mouse hippocampus during long-term spatial memory formation using the reference memory version of radial arm maze (RAM) paradigm. We identified 1592 proteins, which exhibited a complex picture of expression changes during spatial memory formation. Variable linear decomposition reduced significantly data dimensionality and enriched three principal factors responsible for variance of memory-related protein levels at (1) the initial phase of memory acquisition (165 proteins), (2) during the steep learning improvement (148 proteins), and (3) the final phase of the learning curve (123 proteins). Gene ontology and signaling pathways analysis revealed a clear correlation between memory improvement and learning phase-curbed expression profiles of proteins belonging to specific functional categories. We found differential enrichment of (1) neurotrophic factors signaling pathways, proteins regulating synaptic transmission, and actin microfilament during the first day of the learning curve; (2) transcription and translation machinery, protein

PECAN: Library Free Peptide Detection for Data-Independent Acquisition Tandem Mass Spectrometry Data

PubMed Central

Ting, Ying S.; Egertson, Jarrett D.; Bollinger, James G.; Searle, Brian C.; Payne, Samuel H.; Noble, William Stafford; MacCoss, Michael J.

2017-01-01

In mass spectrometry-based shogun proteomics, data-independent acquisition (DIA) is an emerging technique for unbiased and reproducible measurement of protein mixtures. Without targeting a specific precursor ion, DIA MS/MS spectra are often highly multiplexed, containing product ions from multiple co-fragmenting precursors. Thus, detecting peptides directly from DIA data is challenging; most DIA data analyses require spectral libraries. Here we present a new library-free, peptide-centric tool PECAN that detects peptides directly from DIA data. PECAN reports evidence of detection based on product ion scoring, enabling detection of low abundance analytes with poor precursor ion signal. We benchmarked PECAN with chromatographic peak picking accuracy and peptide detection capability. We further validated PECAN detection with data-dependent acquisition and targeted analyses. Last, we used PECAN to build a library from DIA data and to query sequence variants. Together, these results show that PECAN detects peptides robustly and accurately from DIA data without using a library. PMID:28783153

Automation of Vapor-Diffusion Growth of Protein Crystals

NASA Technical Reports Server (NTRS)

Hamrick, David T.; Bray, Terry L.

2005-01-01

Some improvements have been made in a system of laboratory equipment developed previously for studying the crystallization of proteins from solution by use of dynamically controlled flows of dry gas. The improvements involve mainly (1) automation of dispensing of liquids for starting experiments, (2) automatic control of drying of protein solutions during the experiments, and (3) provision for automated acquisition of video images for monitoring experiments in progress and for post-experiment analysis. The automation of dispensing of liquids was effected by adding an automated liquid-handling robot that can aspirate source solutions and dispense them in either a hanging-drop or a sitting-drop configuration, whichever is specified, in each of 48 experiment chambers. A video camera of approximately the size and shape of a lipstick dispenser was added to a mobile stage that is part of the robot, in order to enable automated acquisition of images in each experiment chamber. The experiment chambers were redesigned to enable the use of sitting drops, enable backlighting of each specimen, and facilitate automation.

Knowledge Acquisition in Observational Astronomy.

ERIC Educational Resources Information Center

Vosniadou, Stella

This paper presents findings from research on knowledge acquisition in observational astronomy to demonstrate the kinds of intuitive models children form and to show how these models influence the acquisition of science knowledge. Sixty children of approximate ages 6, 9, and 12 were given a questionnaire to investigate their knowledge of the size,…

Acquisition of Crosswell Seismic Monitoring Data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Daley, T.M.; Niu, F.; Silver, P.G.

2008-02-15

Crosswell seismic acquisition provides an ideal geometry for monitoring travel time changes in the subsurface. Analysis of delay time in terms of a characteristic frequency allows us to estimate optimal acquisition parameters (frequency and distance). We have deployed standard data acquisition equipment for continuous monitoring of crosswell travel time in two separate field experiments, with well spacing of 3 and 30 m. The acquisition hardware used for the field experiments is described, along with environmental effects (such as temperature) that influence the measurements. Two field experiments are described that correlate changes in travel time (and therefore velocity) with changes inmore » barometric pressure. The results from the two field sites show a pressure sensitivity for velocity of 10{sup -6}/Pa to 10{sup -8}/Pa.« less

Manganese acquisition by Lactobacillus plantarum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Archibald, F.S.; Duong, M.N.

1984-04-01

Lactobacillus plantarum has an unusually high Mn(II) requirement for growth and accumulated over 30 mM intracellular Mn(II). The acquisition of Mn(II) by L. plantarum occurred via a specific active transport system powered by the transmembrane proton gradient. The Mn(II) uptake system has a K/sub m/ of 0.2 ..mu..M and a V/sub max/ of 24 nmol mg/sup -1/ of protein min/sup -1/. Above a medium Mn(II) concentration of 200 ..mu..M, the intracellular Mn(II) level was independent of the medium Mn(II) and unresponsive to oxygen stresses but was reduced by phosphate limitation. At a pH of 5.5, citrate, isocitrate, and cis-aconitate effectivelymore » promoted MN(II) uptake, although measurable levels of 1,5-(/sup 14/C)citrate were not accumulated. When cells were presented with equimolar Mn(II) and Cd(II), Cd(II) was preferentially taken up by the Mn(II) transport system. Both Mn(II) and Cd(II) uptake were greatly increased by Mn(II) starvation. Mn(II) uptake by Mn(II)-starved cells was subject to a negative feedback regulatory mechanism functioning less than 1 min after exposure of the cells to Mn(II) and independent of protein synthesis. When presented with a relatively large amount of exogenous Mn(II), Mn(II)-starved cells exhibited a measurable efflux of their internal Mn(II), but the rate was only a small fraction of the maximal Mn(II) uptake rate.« less

Characterization of Spbhp-37, a Hemoglobin-Binding Protein of Streptococcus pneumoniae

PubMed Central

Romero-Espejel, María E.; Rodríguez, Mario A.; Chávez-Munguía, Bibiana; Ríos-Castro, Emmanuel; Olivares-Trejo, José de Jesús

2016-01-01

Streptococcus pneumoniae is a Gram-positive microorganism that is the cause of bacterial pneumonia, sinusitis and otitis media. This human pathogen also can cause invasive diseases such as meningitis, bacteremia and septicemia. Hemoglobin (Hb) and haem can support the growth and viability of S. pneumoniae as sole iron sources. Unfortunately, the acquisition mechanism of Hb and haem in this bacterium has been poorly studied. Previously we identified two proteins of 37 and 22 kDa as putative Hb- and haem-binding proteins (Spbhp-37 and Spbhp-22, respectively). The sequence of Spbhp-37 protein was database annotated as lipoprotein without any function or localization. Here it was immunolocalized in the surface cell by transmission electron microscopy using specific antibodies produced against the recombinant protein. The expression of Spbhp-37 was increased when bacteria were grown in media culture supplied with Hb. In addition, the affinity of Sphbp-37 for Hb was determined. Thus, in this work we are presenting new findings that attempt to explain the mechanism involved in iron acquisition of this pathogen. In the future these results could help to develop new therapy targets in order to avoid the secondary effects caused by the traditional therapies. PMID:27200302

Handbook of Child Language Acquisition.

ERIC Educational Resources Information Center

Ritchie, William C., Ed.; Bhatia, Tej K., Ed.

This volume provides a comprehensive overview of the major areas of research in the field of child language acquisition. It is divided into seven parts and 19 chapters. Part I is an introduction and overview. Part II covers central issues in the study of child language acquisition, focusing on syntax, including those of innateness, maturation, and…

Complex System Governance for Acquisition

DTIC Science & Technology

2016-04-30

2014, September–October). Cybersecurity challenges for program managers . Defense AT&L. Naphade, M., Banavar, G., Harrison, C., Paraszczak, J...the Acquisition Research Program of the Graduate School of Business & Public Policy at the Naval Postgraduate School. To request defense...Dickmann, Vice President, Sonalysts Inc. A Complex Systems Perspective of Risk Mitigation and Modeling in Development and Acquisition Programs Roshanak

Reverse phase protein microarrays: fluorometric and colorimetric detection.

PubMed

Gallagher, Rosa I; Silvestri, Alessandra; Petricoin, Emanuel F; Liotta, Lance A; Espina, Virginia

2011-01-01

The Reverse Phase Protein Microarray (RPMA) is an array platform used to quantitate proteins and their posttranslationally modified forms. RPMAs are applicable for profiling key cellular signaling pathways and protein networks, allowing direct comparison of the activation state of proteins from multiple samples within the same array. The RPMA format consists of proteins immobilized directly on a nitrocellulose substratum. The analyte is subsequently probed with a primary antibody and a series of reagents for signal amplification and detection. Due to the diversity, low concentration, and large dynamic range of protein analytes, RPMAs require stringent signal amplification methods, high quality image acquisition, and software capable of precisely analyzing spot intensities on an array. Microarray detection strategies can be either fluorescent or colorimetric. The choice of a detection system depends on (a) the expected analyte concentration, (b) type of microarray imaging system, and (c) type of sample. The focus of this chapter is to describe RPMA detection and imaging using fluorescent and colorimetric (diaminobenzidine (DAB)) methods.

Managing Uncertainty during a Corporate Acquisition: A Longitudinal Study of Communication During an Airline Acquisition

ERIC Educational Resources Information Center

Kramer, Michael W.; Dougherty, Debbie S.; Pierce, Tamyra A.

2004-01-01

This study examined pilots' (N at T1 = 140; N at T2 = 126; N at T3 = 104) reactions to communication and uncertainty during the acquisition of their airline by another airline. Quantitative results indicate that communication helped to reduce uncertainty and was predictive of affective responses to the acquisition. However, contrary to…

PAnalyzer: a software tool for protein inference in shotgun proteomics.

PubMed

Prieto, Gorka; Aloria, Kerman; Osinalde, Nerea; Fullaondo, Asier; Arizmendi, Jesus M; Matthiesen, Rune

2012-11-05

Protein inference from peptide identifications in shotgun proteomics must deal with ambiguities that arise due to the presence of peptides shared between different proteins, which is common in higher eukaryotes. Recently data independent acquisition (DIA) approaches have emerged as an alternative to the traditional data dependent acquisition (DDA) in shotgun proteomics experiments. MSE is the term used to name one of the DIA approaches used in QTOF instruments. MSE data require specialized software to process acquired spectra and to perform peptide and protein identifications. However the software available at the moment does not group the identified proteins in a transparent way by taking into account peptide evidence categories. Furthermore the inspection, comparison and report of the obtained results require tedious manual intervention. Here we report a software tool to address these limitations for MSE data. In this paper we present PAnalyzer, a software tool focused on the protein inference process of shotgun proteomics. Our approach considers all the identified proteins and groups them when necessary indicating their confidence using different evidence categories. PAnalyzer can read protein identification files in the XML output format of the ProteinLynx Global Server (PLGS) software provided by Waters Corporation for their MSE data, and also in the mzIdentML format recently standardized by HUPO-PSI. Multiple files can also be read simultaneously and are considered as technical replicates. Results are saved to CSV, HTML and mzIdentML (in the case of a single mzIdentML input file) files. An MSE analysis of a real sample is presented to compare the results of PAnalyzer and ProteinLynx Global Server. We present a software tool to deal with the ambiguities that arise in the protein inference process. Key contributions are support for MSE data analysis by ProteinLynx Global Server and technical replicates integration. PAnalyzer is an easy to use multiplatform and

PAnalyzer: A software tool for protein inference in shotgun proteomics

PubMed Central

2012-01-01

Background Protein inference from peptide identifications in shotgun proteomics must deal with ambiguities that arise due to the presence of peptides shared between different proteins, which is common in higher eukaryotes. Recently data independent acquisition (DIA) approaches have emerged as an alternative to the traditional data dependent acquisition (DDA) in shotgun proteomics experiments. MSE is the term used to name one of the DIA approaches used in QTOF instruments. MSE data require specialized software to process acquired spectra and to perform peptide and protein identifications. However the software available at the moment does not group the identified proteins in a transparent way by taking into account peptide evidence categories. Furthermore the inspection, comparison and report of the obtained results require tedious manual intervention. Here we report a software tool to address these limitations for MSE data. Results In this paper we present PAnalyzer, a software tool focused on the protein inference process of shotgun proteomics. Our approach considers all the identified proteins and groups them when necessary indicating their confidence using different evidence categories. PAnalyzer can read protein identification files in the XML output format of the ProteinLynx Global Server (PLGS) software provided by Waters Corporation for their MSE data, and also in the mzIdentML format recently standardized by HUPO-PSI. Multiple files can also be read simultaneously and are considered as technical replicates. Results are saved to CSV, HTML and mzIdentML (in the case of a single mzIdentML input file) files. An MSE analysis of a real sample is presented to compare the results of PAnalyzer and ProteinLynx Global Server. Conclusions We present a software tool to deal with the ambiguities that arise in the protein inference process. Key contributions are support for MSE data analysis by ProteinLynx Global Server and technical replicates integration. PAnalyzer is an

77 FR 189 - Federal Acquisition Regulation; Brand-Name Specifications

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-03

... 2006-0020, Sequence 26] RIN 9000-AK55 Federal Acquisition Regulation; Brand-Name Specifications... changes, the interim rule amending the Federal Acquisition Regulation (FAR) to implement the Office of... for Acquisitions Pursuant to Multiple-Award Contracts, enabled the Civilian Agency Acquisition Council...

48 CFR 32.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... procedures financing. 32.003 Section 32.003 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING 32.003 Simplified acquisition procedures financing. Unless agency regulations otherwise permit, contract financing shall not be provided for...

48 CFR 23.103 - Sustainable acquisitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Sustainable Acquisition Policy 23.103 Sustainable acquisitions. (a... the products are— (1) Energy-efficient (ENERGY STAR® or Federal Energy Management Program (FEMP...

48 CFR 1552.232-74 - Payments-simplified acquisition procedures financing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... acquisition procedures financing. 1552.232-74 Section 1552.232-74 Federal Acquisition Regulations System... Provisions and Clauses 1552.232-74 Payments—simplified acquisition procedures financing. As prescribed in... acquisition procedures financing. Payments—Simplified Acquisition Procedures Financing (JUN 2006) Simplified...

Mergers + acquisitions.

PubMed

Hoppszallern, Suzanna

2002-05-01

The hospital sector in 2001 led the health care field in mergers and acquisitions. Most deals involved a network augmenting its presence within a specific region or in a market adjacent to its primary service area. Analysts expect M&A activity to increase in 2002.

High-throughput single-molecule force spectroscopy for membrane proteins

NASA Astrophysics Data System (ADS)

Bosshart, Patrick D.; Casagrande, Fabio; Frederix, Patrick L. T. M.; Ratera, Merce; Bippes, Christian A.; Müller, Daniel J.; Palacin, Manuel; Engel, Andreas; Fotiadis, Dimitrios

2008-09-01

Atomic force microscopy-based single-molecule force spectroscopy (SMFS) is a powerful tool for studying the mechanical properties, intermolecular and intramolecular interactions, unfolding pathways, and energy landscapes of membrane proteins. One limiting factor for the large-scale applicability of SMFS on membrane proteins is its low efficiency in data acquisition. We have developed a semi-automated high-throughput SMFS (HT-SMFS) procedure for efficient data acquisition. In addition, we present a coarse filter to efficiently extract protein unfolding events from large data sets. The HT-SMFS procedure and the coarse filter were validated using the proton pump bacteriorhodopsin (BR) from Halobacterium salinarum and the L-arginine/agmatine antiporter AdiC from the bacterium Escherichia coli. To screen for molecular interactions between AdiC and its substrates, we recorded data sets in the absence and in the presence of L-arginine, D-arginine, and agmatine. Altogether ~400 000 force-distance curves were recorded. Application of coarse filtering to this wealth of data yielded six data sets with ~200 (AdiC) and ~400 (BR) force-distance spectra in each. Importantly, the raw data for most of these data sets were acquired in one to two days, opening new perspectives for HT-SMFS applications.

Targeting Iron Acquisition Blocks Infection with the Fungal Pathogens Aspergillus fumigatus and Fusarium oxysporum

PubMed Central

Leal, Sixto M.; Roy, Sanhita; Vareechon, Chairut; Carrion, Steven deJesus; Clark, Heather; Lopez-Berges, Manuel S.; diPietro, Antonio; Schrettl, Marcus; Beckmann, Nicola; Redl, Bernhard; Haas, Hubertus; Pearlman, Eric

2013-01-01

Filamentous fungi are an important cause of pulmonary and systemic morbidity and mortality, and also cause corneal blindness and visual impairment worldwide. Utilizing in vitro neutrophil killing assays and a model of fungal infection of the cornea, we demonstrated that Dectin-1 dependent IL-6 production regulates expression of iron chelators, heme and siderophore binding proteins and hepcidin in infected mice. In addition, we show that human neutrophils synthesize lipocalin-1, which sequesters fungal siderophores, and that topical lipocalin-1 or lactoferrin restricts fungal growth in vivo. Conversely, we show that exogenous iron or the xenosiderophore deferroxamine enhances fungal growth in infected mice. By examining mutant Aspergillus and Fusarium strains, we found that fungal transcriptional responses to low iron levels and extracellular siderophores are essential for fungal growth during infection. Further, we showed that targeting fungal iron acquisition or siderophore biosynthesis by topical application of iron chelators or statins reduces fungal growth in the cornea by 60% and that dual therapy with the iron chelator deferiprone and statins further restricts fungal growth by 75%. Together, these studies identify specific host iron-chelating and fungal iron-acquisition mediators that regulate fungal growth, and demonstrate that therapeutic inhibition of fungal iron acquisition can be utilized to treat topical fungal infections. PMID:23853581

Targeting iron acquisition blocks infection with the fungal pathogens Aspergillus fumigatus and Fusarium oxysporum.

PubMed

Leal, Sixto M; Roy, Sanhita; Vareechon, Chairut; Carrion, Steven deJesus; Clark, Heather; Lopez-Berges, Manuel S; Di Pietro, Antonio; diPietro, Antonio; Schrettl, Marcus; Beckmann, Nicola; Redl, Bernhard; Haas, Hubertus; Pearlman, Eric

2013-01-01

Filamentous fungi are an important cause of pulmonary and systemic morbidity and mortality, and also cause corneal blindness and visual impairment worldwide. Utilizing in vitro neutrophil killing assays and a model of fungal infection of the cornea, we demonstrated that Dectin-1 dependent IL-6 production regulates expression of iron chelators, heme and siderophore binding proteins and hepcidin in infected mice. In addition, we show that human neutrophils synthesize lipocalin-1, which sequesters fungal siderophores, and that topical lipocalin-1 or lactoferrin restricts fungal growth in vivo. Conversely, we show that exogenous iron or the xenosiderophore deferroxamine enhances fungal growth in infected mice. By examining mutant Aspergillus and Fusarium strains, we found that fungal transcriptional responses to low iron levels and extracellular siderophores are essential for fungal growth during infection. Further, we showed that targeting fungal iron acquisition or siderophore biosynthesis by topical application of iron chelators or statins reduces fungal growth in the cornea by 60% and that dual therapy with the iron chelator deferiprone and statins further restricts fungal growth by 75%. Together, these studies identify specific host iron-chelating and fungal iron-acquisition mediators that regulate fungal growth, and demonstrate that therapeutic inhibition of fungal iron acquisition can be utilized to treat topical fungal infections.

Duplicate and conquer: multiple homologs of phosphorus-starvation tolerance 1 enhance phosphorus acquisition and sorghum performance on low-P soils

USDA-ARS?s Scientific Manuscript database

Low soil phosphorus (P) availability is a major constraint for crop production in tropical regions. The rice protein kinase, OsPSTOL1, was previously shown to enhance P acquisition and grain yield in rice under P deficiency. We investigated the role of homologs of OsPSTOL1 in sorghum performance und...

48 CFR 1532.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Simplified acquisition procedures financing. 1532.003 Section 1532.003 Federal Acquisition Regulations System ENVIRONMENTAL.... (c) Procedures for purchases exceeding micropurchase threshold. Contracting officers must secure...

48 CFR 1532.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Simplified acquisition procedures financing. 1532.003 Section 1532.003 Federal Acquisition Regulations System ENVIRONMENTAL.... (c) Procedures for purchases exceeding micropurchase threshold. Contracting officers must secure...

48 CFR 1532.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Simplified acquisition procedures financing. 1532.003 Section 1532.003 Federal Acquisition Regulations System ENVIRONMENTAL.... (c) Procedures for purchases exceeding micropurchase threshold. Contracting officers must secure...

48 CFR 1532.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Simplified acquisition procedures financing. 1532.003 Section 1532.003 Federal Acquisition Regulations System ENVIRONMENTAL.... (c) Procedures for purchases exceeding micropurchase threshold. Contracting officers must secure...

Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature.

PubMed

Tsujino, Soichiro; Tomizaki, Takashi

2016-05-06

Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the structure obtained by the conventional oscillation method for up to a 1.8-Å resolution limit. In particular, the absence of protein crystal damage resulting from the acoustic levitation was carefully established. These results represent a key step towards a fully automated sample handling and measurement pipeline, which has promising prospects for a high acquisition rate and high sample efficiency for room temperature X-ray crystallography.

Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature

NASA Astrophysics Data System (ADS)

Tsujino, Soichiro; Tomizaki, Takashi

2016-05-01

Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the structure obtained by the conventional oscillation method for up to a 1.8-Å resolution limit. In particular, the absence of protein crystal damage resulting from the acoustic levitation was carefully established. These results represent a key step towards a fully automated sample handling and measurement pipeline, which has promising prospects for a high acquisition rate and high sample efficiency for room temperature X-ray crystallography.

Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature

PubMed Central

Tsujino, Soichiro; Tomizaki, Takashi

2016-01-01

Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the structure obtained by the conventional oscillation method for up to a 1.8-Å resolution limit. In particular, the absence of protein crystal damage resulting from the acoustic levitation was carefully established. These results represent a key step towards a fully automated sample handling and measurement pipeline, which has promising prospects for a high acquisition rate and high sample efficiency for room temperature X-ray crystallography. PMID:27150272

Combined Acquisition/Processing For Data Reduction

NASA Astrophysics Data System (ADS)

Kruger, Robert A.

1982-01-01

Digital image processing systems necessarily consist of three components: acquisition, storage/retrieval and processing. The acquisition component requires the greatest data handling rates. By coupling together the acquisition witn some online hardwired processing, data rates and capacities for short term storage can be reduced. Furthermore, long term storage requirements can be reduced further by appropriate processing and editing of image data contained in short term memory. The net result could be reduced performance requirements for mass storage, processing and communication systems. Reduced amounts of data also snouid speed later data analysis and diagnostic decision making.

48 CFR 970.2301 - Sustainable acquisition.

Code of Federal Regulations, 2010 CFR

2010-10-01

.... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition...

48 CFR 970.2301 - Sustainable acquisition.

Code of Federal Regulations, 2014 CFR

2014-10-01

.... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition. ...

48 CFR 23.103 - Sustainable acquisitions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Sustainable Acquisition Policy 23.103 Sustainable acquisitions. (a... the products are— (1) Energy-efficient (ENERGY STAR ® or Federal Energy Management Program (FEMP...

48 CFR 970.2301 - Sustainable acquisition.

Code of Federal Regulations, 2012 CFR

2012-10-01

.... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition. ...

48 CFR 970.2301 - Sustainable acquisition.

Code of Federal Regulations, 2013 CFR

2013-10-01

.... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition. ...

48 CFR 970.2301 - Sustainable acquisition.

Code of Federal Regulations, 2011 CFR

2011-10-01

.... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition. ...

48 CFR 208.7403 - Acquisition procedures.

Code of Federal Regulations, 2010 CFR

2010-10-01

... SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES AND SERVICES Enterprise Software Agreements 208.7403 Acquisition procedures. Follow the procedures at PGI 208.7403 when acquiring commercial software and related services. [71 FR 39005, July 11, 2006] ...

β-Adrenergic Receptors Regulate the Acquisition and Consolidation Phases of Aversive Memory Formation Through Distinct, Temporally Regulated Signaling Pathways

PubMed Central

Schiff, Hillary C; Johansen, Joshua P; Hou, Mian; Bush, David E A; Smith, Emily K; Klein, JoAnna E; LeDoux, Joseph E; Sears, Robert M

2017-01-01

Memory formation requires the temporal coordination of molecular events and cellular processes following a learned event. During Pavlovian threat (fear) conditioning (PTC), sensory and neuromodulatory inputs converge on post-synaptic neurons within the lateral nucleus of the amygdala (LA). By activating an intracellular cascade of signaling molecules, these G-protein-coupled neuromodulatory receptors are capable of recruiting a diverse profile of plasticity-related proteins. Here we report that norepinephrine, through its actions on β-adrenergic receptors (βARs), modulates aversive memory formation following PTC through two molecularly and temporally distinct signaling mechanisms. Specifically, using behavioral pharmacology and biochemistry in adult rats, we determined that βAR activity during, but not after PTC training initiates the activation of two plasticity-related targets: AMPA receptors (AMPARs) for memory acquisition and short-term memory and extracellular regulated kinase (ERK) for consolidating the learned association into a long-term memory. These findings reveal that βAR activity during, but not following PTC sets in motion cascading molecular events for the acquisition (AMPARs) and subsequent consolidation (ERK) of learned associations. PMID:27762270

β-Adrenergic Receptors Regulate the Acquisition and Consolidation Phases of Aversive Memory Formation Through Distinct, Temporally Regulated Signaling Pathways.

PubMed

Schiff, Hillary C; Johansen, Joshua P; Hou, Mian; Bush, David E A; Smith, Emily K; Klein, JoAnna E; LeDoux, Joseph E; Sears, Robert M

2017-03-01

Memory formation requires the temporal coordination of molecular events and cellular processes following a learned event. During Pavlovian threat (fear) conditioning (PTC), sensory and neuromodulatory inputs converge on post-synaptic neurons within the lateral nucleus of the amygdala (LA). By activating an intracellular cascade of signaling molecules, these G-protein-coupled neuromodulatory receptors are capable of recruiting a diverse profile of plasticity-related proteins. Here we report that norepinephrine, through its actions on β-adrenergic receptors (βARs), modulates aversive memory formation following PTC through two molecularly and temporally distinct signaling mechanisms. Specifically, using behavioral pharmacology and biochemistry in adult rats, we determined that βAR activity during, but not after PTC training initiates the activation of two plasticity-related targets: AMPA receptors (AMPARs) for memory acquisition and short-term memory and extracellular regulated kinase (ERK) for consolidating the learned association into a long-term memory. These findings reveal that βAR activity during, but not following PTC sets in motion cascading molecular events for the acquisition (AMPARs) and subsequent consolidation (ERK) of learned associations.

Development of a data independent acquisition mass spectrometry workflow to enable glycopeptide analysis without predefined glycan compositional knowledge.

PubMed

Lin, Chi-Hung; Krisp, Christoph; Packer, Nicolle H; Molloy, Mark P

2018-02-10

Glycoproteomics investigates glycan moieties in a site specific manner to reveal the functional roles of protein glycosylation. Identification of glycopeptides from data-dependent acquisition (DDA) relies on high quality MS/MS spectra of glycopeptide precursors and often requires manual validation to ensure confident assignments. In this study, we investigated pseudo-MRM (MRM-HR) and data-independent acquisition (DIA) as alternative acquisition strategies for glycopeptide analysis. These approaches allow data acquisition over the full MS/MS scan range allowing data re-analysis post-acquisition, without data re-acquisition. The advantage of MRM-HR over DDA for N-glycopeptide detection was demonstrated from targeted analysis of bovine fetuin where all three N-glycosylation sites were detected, which was not the case with DDA. To overcome the duty cycle limitation of MRM-HR acquisition needed for analysis of complex samples such as plasma we trialed DIA. This allowed development of a targeted DIA method to identify N-glycopeptides without pre-defined knowledge of the glycan composition, thus providing the potential to identify N-glycopeptides with unexpected structures. This workflow was demonstrated by detection of 59 N-glycosylation sites from 41 glycoproteins from a HILIC enriched human plasma tryptic digest. 21 glycoforms of IgG1 glycopeptides were identified including two truncated structures that are rarely reported. We developed a data-independent mass spectrometry workflow to identify specific glycopeptides from complex biological mixtures. The novelty is that this approach does not require glycan composition to be pre-defined, thereby allowing glycopeptides carrying unexpected glycans to be identified. This is demonstrated through the analysis of immunoglobulins in human plasma where we detected two IgG1 glycoforms that are rarely observed. Copyright © 2017 Elsevier B.V. All rights reserved.

48 CFR 1480.102 - Buy Indian Act acquisition regulations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Buy Indian Act acquisition... INDIAN AFFAIRS SUPPLEMENT ACQUISITIONS UNDER THE BUY INDIAN ACT General 1480.102 Buy Indian Act... Interior Acquisition Regulation (DIAR) requirements to satisfy the needs of Indian Affairs in implementing...

Multiple ABC transporters are involved in the acquisition of petrobactin in Bacillus anthracis

PubMed Central

Dixon, Shandee D.; Janes, Brian K.; Bourgis, Alexandra; Carlson, Paul E.; Hanna, Philip C.

2012-01-01

Summary In Bacillus anthracis the siderophore petrobactin is vital for iron acquisition and virulence. The petrobactin-binding receptor FpuA is required for these processes. Here additional components of petrobactin reacquisition are described. To identify these proteins, mutants of candidate permease and ATPase genes were generated allowing for characterization of multiple petrobactin ATP-binding cassette (ABC)-import systems. Either of two distinct permeases, FpuB or FatCD, are required for iron acquisition and play redundant roles in petrobactin transport. A mutant strain lacking both permeases, ΔfpuBΔfatCD, was incapable of using petrobactin as an iron source and exhibited attenuated virulence in a murine model of inhalational anthrax infection. ATPase mutants were generated in either of the permease mutant backgrounds to identify the ATPase(s) interacting with each individual permease channel. Mutants lacking the FpuB permease and FatE ATPase (ΔfpuBΔfatE) and a mutant lacking the distinct ATPases FpuC and FpuD generated in the ΔfatCD background (ΔfatCDΔfpuCΔfpuD) displayed phenotypic characteristics of a mutant deficient in petrobactin import. A mutant lacking all three of the identified ATPases (ΔfatEΔfpuCΔfpuD) exhibited the same growth defect in iron-depleted conditions. Taken together, these results provide the first description of the permease and ATPase proteins required for the import of petrobactin in B. anthracis. PMID:22429808

O antigen modulates insect vector acquisition of the bacterial plant pathogen Xylella fastidiosa.

PubMed

Rapicavoli, Jeannette N; Kinsinger, Nichola; Perring, Thomas M; Backus, Elaine A; Shugart, Holly J; Walker, Sharon; Roper, M Caroline

2015-12-01

Hemipteran insect vectors transmit the majority of plant pathogens. Acquisition of pathogenic bacteria by these piercing/sucking insects requires intimate associations between the bacterial cells and insect surfaces. Lipopolysaccharide (LPS) is the predominant macromolecule displayed on the cell surface of Gram-negative bacteria and thus mediates bacterial interactions with the environment and potential hosts. We hypothesized that bacterial cell surface properties mediated by LPS would be important in modulating vector-pathogen interactions required for acquisition of the bacterial plant pathogen Xylella fastidiosa, the causative agent of Pierce's disease of grapevines. Utilizing a mutant that produces truncated O antigen (the terminal portion of the LPS molecule), we present results that link this LPS structural alteration to a significant decrease in the attachment of X. fastidiosa to blue-green sharpshooter foreguts. Scanning electron microscopy confirmed that this defect in initial attachment compromised subsequent biofilm formation within vector foreguts, thus impairing pathogen acquisition. We also establish a relationship between O antigen truncation and significant changes in the physiochemical properties of the cell, which in turn affect the dynamics of X. fastidiosa adhesion to the vector foregut. Lastly, we couple measurements of the physiochemical properties of the cell with hydrodynamic fluid shear rates to produce a Comsol model that predicts primary areas of bacterial colonization within blue-green sharpshooter foreguts, and we present experimental data that support the model. These results demonstrate that, in addition to reported protein adhesin-ligand interactions, O antigen is crucial for vector-pathogen interactions, specifically in the acquisition of this destructive agricultural pathogen. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

The Defense Systems Acquisition and Review Council

DTIC Science & Technology

1976-09-15

THE DEFENSE SYSTEMS ACQUISITION AND REVIEW COUNCIL.. A Study of Areas of Consideration Affecting the Functions and Process of Defense Major...COUNfIL: 4 Study of Areas of Considerationi Affecting/he Functions and Process of Defense _- 1 Major Systems Acquisition. , ; O,. v AUTHOR(e) I. C...Studies DSARC -- Functions and Process OSDCAIG *, Army Systems . A - =A -- he Defense Systems Acquisition Review Council (DSARC) was created to assume

High Speed Intact Protein Characterization Using 4X Frequency Multiplication, Ion Trap Harmonization, and 21 Tesla FTICR-MS.

PubMed

Shaw, Jared B; Gorshkov, Mikhail V; Wu, Qinghao; Paša-Tolić, Ljiljana

2018-05-01

Mass spectrometric characterization of large biomolecules, such as intact proteins, requires the specificity afforded by ultrahigh resolution mass measurements performed at both the intact mass and product ion levels. Although the performance of time-of-flight mass analyzers is steadily increasing, the choice of mass analyzer for large biomolecules (e.g., proteins >50 kDa) is generally limited to the Fourier transform family of mass analyzers such as Orbitrap and ion cyclotron resonance (FTICR-MS), with the latter providing unmatched mass resolving power and measurement accuracy. Yet, protein analyses using FTMS are largely hindered by the low acquisition rates of spectra with ultrahigh resolving power. Frequency multiple detection schemes enable FTICR-MS to overcome this fundamental barrier and achieve resolving powers and acquisition speeds 4× greater than the limits imposed by magnetic field strength. Here we expand upon earlier work on the implementation of this technique for biomolecular characterization. We report the coupling of 21T FTICR-MS, 4X frequency multiplication, ion trapping field harmonization technology, and spectral data processing methods to achieve unprecedented acquisition rates and resolving power in mass spectrometry of large intact proteins. Isotopically resolved spectra of multiply charged ubiquitin ions were acquired using detection periods as short as 12 ms. Large proteins such as apo-transferrin (MW = 78 kDa) and monoclonal antibody (MW = 150 kDa) were isotopically resolved with detection periods of 384 and 768 ms, respectively. These results illustrate the future capability of accurate characterization of large proteins on time scales compatible with online separations.

10 CFR 490.302 - Vehicle acquisition mandate schedule.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 3 2012-01-01 2012-01-01 false Vehicle acquisition mandate schedule. 490.302 Section 490.302 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.302 Vehicle acquisition mandate schedule. (a...

10 CFR 490.305 - Acquisitions satisfying the mandate.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 3 2012-01-01 2012-01-01 false Acquisitions satisfying the mandate. 490.305 Section 490.305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The...

10 CFR 490.302 - Vehicle acquisition mandate schedule.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 3 2010-01-01 2010-01-01 false Vehicle acquisition mandate schedule. 490.302 Section 490.302 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.302 Vehicle acquisition mandate schedule. (a...

10 CFR 490.305 - Acquisitions satisfying the mandate.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 3 2011-01-01 2011-01-01 false Acquisitions satisfying the mandate. 490.305 Section 490.305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The...

10 CFR 490.305 - Acquisitions satisfying the mandate.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 3 2013-01-01 2013-01-01 false Acquisitions satisfying the mandate. 490.305 Section 490.305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The...

10 CFR 490.305 - Acquisitions satisfying the mandate.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 3 2014-01-01 2014-01-01 false Acquisitions satisfying the mandate. 490.305 Section 490.305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The...

10 CFR 490.302 - Vehicle acquisition mandate schedule.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 3 2011-01-01 2011-01-01 false Vehicle acquisition mandate schedule. 490.302 Section 490.302 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.302 Vehicle acquisition mandate schedule. (a...

10 CFR 490.302 - Vehicle acquisition mandate schedule.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 3 2013-01-01 2013-01-01 false Vehicle acquisition mandate schedule. 490.302 Section 490.302 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.302 Vehicle acquisition mandate schedule. (a...

10 CFR 490.302 - Vehicle acquisition mandate schedule.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 3 2014-01-01 2014-01-01 false Vehicle acquisition mandate schedule. 490.302 Section 490.302 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.302 Vehicle acquisition mandate schedule. (a...

DNA and virus particle vaccination protects against acquisition and confers control of viremia upon heterologous simian immunodeficiency virus challenge.

PubMed

Patel, Vainav; Jalah, Rashmi; Kulkarni, Viraj; Valentin, Antonio; Rosati, Margherita; Alicea, Candido; von Gegerfelt, Agneta; Huang, Wensheng; Guan, Yongjun; Keele, Brandon F; Bess, Julian W; Piatak, Michael; Lifson, Jeffrey D; Williams, William T; Shen, Xiaoying; Tomaras, Georgia D; Amara, Rama R; Robinson, Harriet L; Johnson, Welkin; Broderick, Kate E; Sardesai, Niranjan Y; Venzon, David J; Hirsch, Vanessa M; Felber, Barbara K; Pavlakis, George N

2013-02-19

We have previously shown that macaques vaccinated with DNA vectors expressing SIVmac239 antigens developed potent immune responses able to reduce viremia upon high-dose SIVmac251 challenge. To further improve vaccine-induced immunity and protection, we combined the SIVmac239 DNA vaccine with protein immunization using inactivated SIVmac239 viral particles as protein source. Twenty-six weeks after the last vaccination, the animals were challenged intrarectally at weekly intervals with a titrated dose of the heterologous SIVsmE660. Two of DNA-protein coimmunized macaques did not become infected after 14 challenges, but all controls were infected by 11 challenges. Vaccinated macaques showed modest protection from SIVsmE660 acquisition compared with naïve controls (P = 0.050; stratified for TRIM5α genotype). Vaccinees had significantly lower peak (1.6 log, P = 0.0048) and chronic phase viremia (P = 0.044), with 73% of the vaccinees suppressing viral replication to levels below assay detection during the 40-wk follow-up. Vaccine-induced immune responses associated significantly with virus control: binding antibody titers and the presence of rectal IgG to SIVsmE660 Env correlated with delayed SIVsmE660 acquisition; SIV-specific cytotoxic T cells, prechallenge CD4(+) effector memory, and postchallenge CD8(+) transitional memory cells correlated with control of viremia. Thus, SIVmac239 DNA and protein-based vaccine protocols were able to achieve high, persistent, broad, and effective cellular and humoral immune responses able to delay heterologous SIVsmE660 infection and to provide long-term control of viremia. These studies support a role of DNA and protein-based vaccines for development of an efficacious HIV/AIDS vaccine.

[Mechanisms of endogenous drug resistance acquisition by spontaneous chromosomal gene mutation].

PubMed

Fukuda, H; Hiramatsu, K

1997-05-01

Endogenous resistance in bacteria is caused by a change or loss of function and generally genetically recessive. However, this type of resistance acquisition are now prevalent in clinical setting. Chromosomal genes that afford endogenous resistance are the genes correlated with the target of the drug, the drug inactivating enzymes, and permeability of the molecules including the antibacterial agents. Endogenous alteration of the drug target are mediated by the spontaneous mutation of their structural gene. This mutation provides much lower affinity of the drugs for the target. Gene expression of the inactivating enzymes, such as class C beta-lactamase, is generally regulated by regulatory genes. Spontaneous mutations in the regulatory genes cause constitutive enzyme production and provides the resistant to the agent which is usually stable for such enzymes. Spontaneous mutation in the structural gene gives the enzyme extra-spectrum substrate specificity, like ESBL (Extra-Spectrum-beta-Lactamase). Expression of structural genes encoding the permeability systems are also regulated by some regulatory genes. The spontaneous mutation of the regulatory genes reduce an amount of porin protein. This mutation causes much lower influx of the drug in the cell. Spontaneous mutation in promoter region of the structural gene of efflux protein was observed. This mutation raised the gene transcription and overproduced efflux protein. This protein progresses the drug efflux from the cell.

48 CFR 952.217-70 - Acquisition of real property.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Acquisition of real... Acquisition of real property. Insert the following clause when required by 917.7403: Acquisition of Real... proposes to acquire use of real property by: (1) Purchase, on the Government's behalf or in the Contractor...

48 CFR 952.217-70 - Acquisition of real property.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Acquisition of real... Acquisition of real property. Insert the following clause when required by 917.7403: Acquisition of Real... proposes to acquire use of real property by: (1) Purchase, on the Government's behalf or in the Contractor...

48 CFR 952.217-70 - Acquisition of real property.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Acquisition of real... Acquisition of real property. Insert the following clause when required by 917.7403: Acquisition of Real... proposes to acquire use of real property by: (1) Purchase, on the Government's behalf or in the Contractor...

48 CFR 952.217-70 - Acquisition of real property.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Acquisition of real... Acquisition of real property. Insert the following clause when required by 917.7403: Acquisition of Real... proposes to acquire use of real property by: (1) Purchase, on the Government's behalf or in the Contractor...

48 CFR 952.217-70 - Acquisition of real property.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Acquisition of real... Acquisition of real property. Insert the following clause when required by 917.7403: Acquisition of Real... proposes to acquire use of real property by: (1) Purchase, on the Government's behalf or in the Contractor...

Intra-Amygdala ZIP Injections Impair the Memory of Learned Active Avoidance Responses and Attenuate Conditioned Taste-Aversion Acquisition in Rats

ERIC Educational Resources Information Center

Gamiz, Fernando; Gallo, Milagros

2011-01-01

We have investigated the effect of protein kinase Mzeta (PKM[zeta]) inhibition in the basolateral amygdala (BLA) upon the retention of a nonspatial learned active avoidance response and conditioned taste-aversion (CTA) acquisition in rats. ZIP (10 nmol/[mu]L) injected into the BLA 24 h after training impaired retention of a learned…

Processes Asunder: Acquisition & Planning Misfits

DTIC Science & Technology

2009-03-26

Establishing six Business Enterprise Priorities ( BEPs ) to focus the Department’s business transformation efforts, which now guide DoD investment decisions...three phases which look very much like Milestone A, B, and C of the previously existing Life Cycle Management Framework . With this obvious redundancy...February 2002). 30 6 Defense Acquisition University, “Integrated Defense Acquisition, Technology, & Logistics Life Cycle Management Framework , version 5.2

48 CFR 1845.7101-3 - Unit acquisition cost.

Code of Federal Regulations, 2010 CFR

2010-10-01

... services for designs, plans, specifications, and surveys. (6) Acquisition and preparation costs of... acquisition cost is under $100,000, it shall be reported as under $100,000. (g) Software acquisition costs include software costs incurred up through acceptance testing and material internal costs incurred to...

Positron Emission Mammography with Multiple Angle Acquisition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mark F. Smith; Stan Majewski; Raymond R. Raylman

2002-11-01

Positron emission mammography (PEM) of F-18 fluorodeoxyglucose (FbG) uptake in breast tumors with dedicated detectors typically has been accomplished with two planar detectors in a fixed position with the breast under compression. The potential use of PEM imaging at two detector positions to guide stereotactic breast biopsy has motivated us to use PEM coincidence data acquired at two or more detector positions together in a single image reconstruction. Multiple angle PEM acquisition and iterative image reconstruction were investigated using point source and compressed breast phantom acquisitions with 5, 9, 12 and 15 mm diameter spheres and a simulated tumor:background activitymore » concentration ratio of 6:1. Image reconstruction was performed with an iterative MLEM algorithm that used coincidence events between any two detector pixels on opposed detector heads at each detector position. This present study compared two acquisition protocols: 2 angle acquisition with detector angular positions of -15 and +15 degrees and 11 angle acquisition with detector positions spaced at 3 degree increments over the range -15 to +15 degrees. Three-dimensional image resolution was assessed for the point source acquisitions, and contrast and signal-to-noise metrics were evaluated for the compressed breast phantom with different simulated tumor sizes. Radial and tangential resolutions were similar for the two protocols, while normal resolution was better for the 2 angle acquisition. Analysis is complicated by the asymmetric point spread functions. Signal- to-noise vs. contrast tradeoffs were better for 11 angle acquisition for the smallest visible 9 mm sphere, while tradeoff results were mixed for the larger and more easily visible 12 mm and 15 mm diameter spheres. Additional study is needed to better understand the performance of limited angle tomography for PEM. PEM tomography experiments with complete angular sampling are planned.« less

Predictors of chain acquisition among independent dialysis facilities.

PubMed

Pozniak, Alyssa S; Hirth, Richard A; Banaszak-Holl, Jane; Wheeler, John R C

2010-04-01

To determine the predictors of chain acquisition among independent dialysis providers. Retrospective facility-level data combined from CMS Cost Reports, Medical Evidence Forms, Annual Facility Surveys, and claims for 1996-2003. Independent dialysis facilities' probability of acquisition by a dialysis chain (overall and by chain size) was estimated using a discrete time hazard rate model, controlling for financial and clinical performance, practice patterns, market factors, and other facility characteristics. The sample includes all U.S. freestanding dialysis facilities that report not being chain affiliated for at least 1 year between 1997 and 2003. Above-average costs and better quality outcomes are significant determinants of dialysis chain acquisition. Facilities in larger markets were more likely to be acquired by a chain. Furthermore, small dialysis chains have different acquisition strategies than large chains. Dialysis chains appear to employ a mix of turn-around and cream-skimming strategies. Poor financial health is a predictor of chain acquisition as in other health care sectors, but the increased likelihood of chain acquisition among higher quality facilities is unique to the dialysis industry. Significant differences among predictors of acquisition by small and large chains reinforce the importance of using a richer classification for chain status.

48 CFR 1034.004 - Acquisition strategy.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Order, Task Order, or Interagency Agreement) to the overall investment requirements and management... investment; (3) A description of the effort, by acquisition, and the plans to include required clauses in the... requirements to manage the acquisition processes through the investment lifecycle; (7) Consideration of optimal...

Survey identifies trends in equipment acquisitions.

PubMed

Anderson, H J

1990-09-20

Automated laboratory equipment, medical imaging equipment, and patient monitors top the list of hospitals' priorities for equipment acquisition during the next year. This is according to an exclusive survey sponsored by the Linc Group, Inc., Chicago, and Hospitals magazine. The new survey also found that equipment acquisitions are most often financed with internal funds and gifts; that equipment purchasing budgets are on the rise; and that executives believe that most equipment makes money for their hospitals. Find out what your peers have to say about anticipated trends in equipment acquisition and financing during the next year.

Societal Responses to Adult Difficulties in L2 Acquisition: Toward an Evolutionary Perspective on Language Acquisition

ERIC Educational Resources Information Center

Schumann, John H.

2013-01-01

It is generally accepted that second language (L2) acquisition becomes more difficult as one grows older and that success in adult L2 acquisition is highly variable. Nevertheless, humans in language contact situations have to cope with intergroup communication. This article examines the ways society has responded to this challenge. It describes…

Multi-disciplinary methods to define RNA-protein interactions and regulatory networks.

PubMed

Ascano, Manuel; Gerstberger, Stefanie; Tuschl, Thomas

2013-02-01

The advent of high-throughput technologies including deep-sequencing and protein mass spectrometry is facilitating the acquisition of large and precise data sets toward the definition of post-transcriptional regulatory networks. While early studies that investigated specific RNA-protein interactions in isolation laid the foundation for our understanding of the existence of molecular machines to assemble and process RNAs, there is a more recent appreciation of the importance of individual RNA-protein interactions that contribute to post-transcriptional gene regulation. The multitude of RNA-binding proteins (RBPs) and their many RNA targets has only been captured experimentally in recent times. In this review, we will examine current multidisciplinary approaches toward elucidating RNA-protein networks and their regulation. Copyright © 2013 Elsevier Ltd. All rights reserved.

Glycolipid Acquisition by Human Glycolipid Transfer Protein Dramatically Alters Intrinsic Tryptophan Fluorescence

PubMed Central

Zhai, Xiuhong; Malakhova, Margarita L.; Pike, Helen M.; Benson, Linda M.; Bergen, H. Robert; Sugár, István P.; Malinina, Lucy; Patel, Dinshaw J.; Brown, Rhoderick E.

2009-01-01

Glycolipid transfer proteins (GLTPs) are small, soluble proteins that selectively accelerate the intermembrane transfer of glycolipids. The GLTP fold is conformationally unique among lipid binding/transfer proteins and serves as the prototype and founding member of the new GLTP superfamily. In the present study, changes in human GLTP tryptophan fluorescence, induced by membrane vesicles containing glycolipid, are shown to reflect glycolipid binding when vesicle concentrations are low. Characterization of the glycolipid-induced “signature response,” i.e. ∼40% decrease in Trp intensity and ∼12-nm blue shift in emission wavelength maximum, involved various modes of glycolipid presentation, i.e. microinjection/dilution of lipid-ethanol solutions or phosphatidylcholine vesicles, prepared by sonication or extrusion and containing embedded glycolipids. High resolution x-ray structures of apo- and holo-GLTP indicate that major conformational alterations are not responsible for the glycolipid-induced GLTP signature response. Instead, glycolipid binding alters the local environment of Trp-96, which accounts for ∼70% of total emission intensity of three Trp residues in GLTP and provides a stacking platform that aids formation of a hydrogen bond network with the ceramide-linked sugar of the glycolipid headgroup. The changes in Trp signal were used to quantitatively assess human GLTP binding affinity for various lipids including glycolipids containing different sugar headgroups and homogenous acyl chains. The presence of the glycolipid acyl chain and at least one sugar were essential for achieving a low-to-submicromolar dissociation constant that was only slightly altered by increased sugar headgroup complexity. PMID:19270338

Child Language Acquisition: Contrasting Theoretical Approaches

ERIC Educational Resources Information Center

Ambridge, Ben; Lieven, Elena V. M.

2011-01-01

Is children's language acquisition based on innate linguistic structures or built from cognitive and communicative skills? This book summarises the major theoretical debates in all of the core domains of child language acquisition research (phonology, word-learning, inflectional morphology, syntax and binding) and includes a complete introduction…

Acquisition by Processing Theory: A Theory of Everything?

ERIC Educational Resources Information Center

Carroll, Susanne E.

2004-01-01

Truscott and Sharwood Smith (henceforth T&SS) propose a novel theory of language acquisition, "Acquisition by Processing Theory" (APT), designed to account for both first and second language acquisition, monolingual and bilingual speech perception and parsing, and speech production. This is a tall order. Like any theoretically ambitious…

75 FR 82576 - Federal Acquisition Regulation; Terminating Contracts

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-30

... Acquisition Council (Councils) agree. The language at FAR 49.501, pertaining to the applicability of FAR part...-AL56 Federal Acquisition Regulation; Terminating Contracts AGENCY: Department of Defense (DoD), General.... SUMMARY: DoD, GSA, and NASA are issuing a final rule amending the Federal Acquisition Regulation (FAR) to...

48 CFR 225.7303 - Pricing acquisitions for FMS.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Pricing acquisitions for... Military Sales 225.7303 Pricing acquisitions for FMS. (a) Price FMS contracts using the same principles used in pricing other defense contracts. However, application of the pricing principles in FAR parts 15...

48 CFR 53.212 - Acquisition of commercial items.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Acquisition of commercial... (CONTINUED) CLAUSES AND FORMS FORMS Prescription of Forms 53.212 Acquisition of commercial items. SF 1449 (Rev. 3/2005), Solicitation/Contract/Order for Commercial Items. SF 1449 is prescribed for use in...

Peruvian Weapon System Acquisition Process

DTIC Science & Technology

1990-12-01

East bloc nations such as Rumania, Bulgaria, Poland, and Yugoslavia essentially retained all or much of their command economies. As these nations foray...The level of technology transfer is an absolut* essential determent for dictating the rate and complexity of Peruvian technologia advancement in the...cases greater than the acquisition cost, it is essential that such costs be considered in development and acquisition decisions in order that proper

Acquisition and tracking for underwater optical communications

NASA Astrophysics Data System (ADS)

Williams, Andrew J.; Laycock, Leslie L.; Griffith, Michael S.; McCarthy, Andrew G.; Rowe, Duncan P.

2017-10-01

There is a growing requirement to transfer large volumes of data between underwater platforms. As seawater is transmissive in the visible band, underwater optical communications is an active area of interest since it offers the potential for power efficient, covert and high bandwidth datalinks at short to medium ranges. Short range systems have been successfully demonstrated using sources with low directionality. To realise higher data rates and/or longer ranges, the use of more efficient directional beams is required; by necessity, these must be sufficiently aligned to achieve the required link margin. For mobile platforms, the acquisition and tracking of each node is therefore critical in order to establish and maintain an optical datalink. This paper describes work undertaken to demonstrate acquisition and tracking in a 3D underwater environment. A range of optical sources, beam steering technologies, and tracking sensors have been assessed for suitability. A novel scanning strategy exploiting variable beam divergence was developed to provide robust acquisition whilst minimising acquisition time. A prototype system was assembled and demonstrated in a large water tank. This utilised custom quadrant detectors based on Silicon PhotoMultiplier (SiPM) arrays for fine tracking, and a Wide Field of View (WFoV) sCMOS camera for link acquisition. Fluidic lenses provided dynamic control of beam divergence, and AC modulation/filtering enabled background rejection. The system successfully demonstrated robust optical acquisition and tracking between two nodes with only nanowatt received optical powers. The acquisition time was shown to be dependent on the initial conditions and the transmitted optical power.

Two Demonstrations with a New Data-Acquisition System

ERIC Educational Resources Information Center

Kraftmakher, Yaakov

2014-01-01

Nowadays, the use of data-acquisition systems in undergraduate laboratories is routine. Many computer-assisted experiments became possible with the PASCO scientific data-acquisition system based on the 750 Interface and DataStudio software. A new data-acquisition system developed by PASCO includes the 850 Universal Interface and Capstone software.…

16 CFR 802.35 - Acquisitions by employee trusts.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Acquisitions by employee trusts. 802.35... Acquisitions by employee trusts. An acquisition of voting securities shall be exempt from the notification requirements of the act if: (a) The securities are acquired by a trust that meets the qualifications of section...

Input and Intake in Language Acquisition

ERIC Educational Resources Information Center

Gagliardi, Ann C.

2012-01-01

This dissertation presents an approach for a productive way forward in the study of language acquisition, sealing the rift between claims of an innate linguistic hypothesis space and powerful domain general statistical inference. This approach breaks language acquisition into its component parts, distinguishing the input in the environment from…

Second Language Acquisition and Universal Grammar.

ERIC Educational Resources Information Center

White, Lydia

1990-01-01

Discusses the motivation for Universal Grammar (UG), as assumed in the principles and parameters framework of generative grammar (Chomsky, 1981), focusing on the logical problem of first-language acquisition and the potential role of UG in second-language acquisition. Recent experimental research regarding the second-language status of the…

48 CFR 225.7003 - Restrictions on acquisition of specialty metals.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Restrictions on acquisition of specialty metals. 225.7003 Section 225.7003 Federal Acquisition Regulations System DEFENSE... Restrictions on acquisition of specialty metals. ...

48 CFR 225.7003 - Restrictions on acquisition of specialty metals.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Restrictions on acquisition of specialty metals. 225.7003 Section 225.7003 Federal Acquisition Regulations System DEFENSE... Restrictions on acquisition of specialty metals. ...

76 FR 27274 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-11

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 225 Defense Federal Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations System... Federal Acquisition Regulation Supplement (DFARS) to direct contracting officers to additional guidance on...

Predictors of Chain Acquisition among Independent Dialysis Facilities

PubMed Central

Pozniak, Alyssa S; Hirth, Richard A; Banaszak-Holl, Jane; Wheeler, John R C

2010-01-01

Objective To determine the predictors of chain acquisition among independent dialysis providers. Data Sources Retrospective facility-level data combined from CMS Cost Reports, Medical Evidence Forms, Annual Facility Surveys, and claims for 1996–2003. Study Design Independent dialysis facilities' probability of acquisition by a dialysis chain (overall and by chain size) was estimated using a discrete time hazard rate model, controlling for financial and clinical performance, practice patterns, market factors, and other facility characteristics. Data Collection The sample includes all U.S. freestanding dialysis facilities that report not being chain affiliated for at least 1 year between 1997 and 2003. Principal Findings Above-average costs and better quality outcomes are significant determinants of dialysis chain acquisition. Facilities in larger markets were more likely to be acquired by a chain. Furthermore, small dialysis chains have different acquisition strategies than large chains. Conclusions Dialysis chains appear to employ a mix of turn-around and cream-skimming strategies. Poor financial health is a predictor of chain acquisition as in other health care sectors, but the increased likelihood of chain acquisition among higher quality facilities is unique to the dialysis industry. Significant differences among predictors of acquisition by small and large chains reinforce the importance of using a richer classification for chain status. PMID:20148985

Drug spend and acquisitive offending by substance misusers.

PubMed

Hayhurst, Karen P; Jones, Andrew; Millar, Tim; Pierce, Matthias; Davies, Linda; Weston, Samantha; Donmall, Michael

2013-06-01

The need to generate income to fund drug misuse is assumed to be a driver of involvement in acquisitive crime. We examined the influence of drug misuse expenditure, and other factors, on acquisitive offending. Clients (N=1380) seeking drug treatment within 94 of 149 Drug Action Teams (DATs) across England completed a comprehensive survey, incorporating validated scales and self-report measures, such as levels of drug and alcohol use and offending. Forty per cent (N=554) had committed acquisitive crime in the previous month. Regression analysis showed that acquisitive offending was associated with the presence of problematic use of crack cocaine, poly-drug use, sharing injecting equipment, unsafe sex, overdose risk, higher drug spend, unemployment, reduced mental wellbeing, and younger age. Rates of acquisitive crime among drug users are high. Drug using offenders can be distinguished from drug using non-offenders by problematic crack cocaine use, younger age, income-related factors, and indicators of a chaotic life style and complex needs. Behavioural and demographic factors were associated more strongly with acquisitive crime than drug use expenditure, suggesting that the need to finance drug use is not necessarily the main factor driving acquisitive offending by drug users. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

48 CFR 225.7702 - Acquisition of small arms.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Acquisition of small arms... Operations in Iraq or Afghanistan 225.7702 Acquisition of small arms. (a) Except as provided in paragraph (b) of this section, when acquiring small arms for assistance to the Army of Iraq, the Army of...

48 CFR 225.7702 - Acquisition of small arms.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Acquisition of small arms... Operations in Iraq or Afghanistan 225.7702 Acquisition of small arms. (a) Except as provided in paragraph (b) of this section, when acquiring small arms for assistance to the Army of Iraq, the Army of...

48 CFR 225.7702 - Acquisition of small arms.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Acquisition of small arms... Operations in Iraq or Afghanistan 225.7702 Acquisition of small arms. (a) Except as provided in paragraph (b) of this section, when acquiring small arms for assistance to the Army of Iraq, the Army of...

75 FR 78619 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-16

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Parts 216 and 237 Defense Federal Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations... the Defense Federal Acquisition Regulation Supplement (DFARS) to set forth references to supplementary...

A high speed buffer for LV data acquisition

NASA Technical Reports Server (NTRS)

Cavone, Angelo A.; Sterlina, Patrick S.; Clemmons, James I., Jr.; Meyers, James F.

1987-01-01

The laser velocimeter (autocovariance) buffer interface is a data acquisition subsystem designed specifically for the acquisition of data from a laser velocimeter. The subsystem acquires data from up to six laser velocimeter components in parallel, measures the times between successive data points for each of the components, establishes and maintains a coincident condition between any two or three components, and acquires data from other instrumentation systems simultaneously with the laser velocimeter data points. The subsystem is designed to control the entire data acquisition process based on initial setup parameters obtained from a host computer and to be independent of the computer during the acquisition. On completion of the acquisition cycle, the interface transfers the contents of its memory to the host under direction of the host via a single 16-bit parallel DMA channel.

76 FR 39234 - Federal Acquisition Regulation; Unique Procurement Instrument Identifier

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-05

...-AL70 Federal Acquisition Regulation; Unique Procurement Instrument Identifier AGENCIES: Department of... Acquisition Regulation (FAR) to standardize use of unique Procurement Instrument Identifiers (PIID) throughout... Acquisition Council and the Defense Acquisition Regulations Council (the Councils) reviewed the public...

48 CFR 1401.370 - Acquisition Managers' Partnership.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Acquisition Managers' Partnership. (a) The Acquisition Managers' Partnership (AMP) is a forum for DOI's senior... and effectiveness of its procurement services in support of DOI's mission. (b) The AMP consists of the...

7 CFR 1710.107 - Amount lent for acquisitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false Amount lent for acquisitions. 1710.107 Section 1710... GUARANTEES Loan Purposes and Basic Policies § 1710.107 Amount lent for acquisitions. The maximum amount that will be lent for an acquisition is limited to the value of the property, as determined by RUS. If the...

Cross-linguistic patterns in the acquisition of quantifiers.

PubMed

Katsos, Napoleon; Cummins, Chris; Ezeizabarrena, Maria-José; Gavarró, Anna; Kuvač Kraljević, Jelena; Hrzica, Gordana; Grohmann, Kleanthes K; Skordi, Athina; Jensen de López, Kristine; Sundahl, Lone; van Hout, Angeliek; Hollebrandse, Bart; Overweg, Jessica; Faber, Myrthe; van Koert, Margreet; Smith, Nafsika; Vija, Maigi; Zupping, Sirli; Kunnari, Sari; Morisseau, Tiffany; Rusieshvili, Manana; Yatsushiro, Kazuko; Fengler, Anja; Varlokosta, Spyridoula; Konstantzou, Katerina; Farby, Shira; Guasti, Maria Teresa; Vernice, Mirta; Okabe, Reiko; Isobe, Miwa; Crosthwaite, Peter; Hong, Yoonjee; Balčiūnienė, Ingrida; Ahmad Nizar, Yanti Marina; Grech, Helen; Gatt, Daniela; Cheong, Win Nee; Asbjørnsen, Arve; Torkildsen, Janne von Koss; Haman, Ewa; Miękisz, Aneta; Gagarina, Natalia; Puzanova, Julia; Anđelković, Darinka; Savić, Maja; Jošić, Smiljana; Slančová, Daniela; Kapalková, Svetlana; Barberán, Tania; Özge, Duygu; Hassan, Saima; Chan, Cecilia Yuet Hung; Okubo, Tomoya; van der Lely, Heather; Sauerland, Uli; Noveck, Ira

2016-08-16

Learners of most languages are faced with the task of acquiring words to talk about number and quantity. Much is known about the order of acquisition of number words as well as the cognitive and perceptual systems and cultural practices that shape it. Substantially less is known about the acquisition of quantifiers. Here, we consider the extent to which systems and practices that support number word acquisition can be applied to quantifier acquisition and conclude that the two domains are largely distinct in this respect. Consequently, we hypothesize that the acquisition of quantifiers is constrained by a set of factors related to each quantifier's specific meaning. We investigate competence with the expressions for "all," "none," "some," "some…not," and "most" in 31 languages, representing 11 language types, by testing 768 5-y-old children and 536 adults. We found a cross-linguistically similar order of acquisition of quantifiers, explicable in terms of four factors relating to their meaning and use. In addition, exploratory analyses reveal that language- and learner-specific factors, such as negative concord and gender, are significant predictors of variation.

75 FR 48873 - Acquisition Regulation Rewrite

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-12

... Acquisition Regulation (DIAR). This action revises the DIAR, 48 CFR chapter 14, but does not impose any new..., 2010. No public comments were received. DOI has concluded that the interim rule should be adopted as a... DEPARTMENT OF THE INTERIOR Office of the Secretary 48 CFR Chapter 14 RIN 1093-AA11 Acquisition...

Molecular Basis for Transmission Barrier and Interference between Closely Related Prion Proteins in Yeast*

PubMed Central

Afanasieva, Evgenia G.; Kushnirov, Vitaly V.; Tuite, Mick F.; Ter-Avanesyan, Michael D.

2011-01-01

Replicating amyloids, called prions, are responsible for transmissible neurodegenerative diseases in mammals and some heritable phenotypes in fungi. The transmission of prions between species is usually inhibited, being highly sensitive to small differences in amino acid sequence of the prion-forming proteins. To understand the molecular basis of this prion interspecies barrier, we studied the transmission of the [PSI+] prion state from Sup35 of Saccharomyces cerevisiae to hybrid Sup35 proteins with prion-forming domains from four other closely related Saccharomyces species. Whereas all the hybrid Sup35 proteins could adopt a prion form in S. cerevisiae, they could not readily acquire the prion form from the [PSI+] prion of S. cerevisiae. Expression of the hybrid Sup35 proteins in S. cerevisiae [PSI+] cells often resulted in frequent loss of the native [PSI+] prion. Furthermore, all hybrid Sup35 proteins showed different patterns of interaction with the native [PSI+] prion in terms of co-polymerization, acquisition of the prion state, and induced prion loss, all of which were also dependent on the [PSI+] variant. The observed loss of S. cerevisiae [PSI+] can be related to inhibition of prion polymerization of S. cerevisiae Sup35 and formation of a non-heritable form of amyloid. We have therefore identified two distinct molecular origins of prion transmission barriers between closely sequence-related prion proteins: first, the inability of heterologous proteins to co-aggregate with host prion polymers, and second, acquisition by these proteins of a non-heritable amyloid fold. PMID:21454674

75 FR 60261 - Federal Acquisition Regulation; Award-Fee Language Revision

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-29

...-AL42 Federal Acquisition Regulation; Award-Fee Language Revision AGENCIES: Department of Defense (DoD...: Final rule. SUMMARY: The Civilian Agency Acquisition Council and the Defense Acquisition Regulations Council (Councils) have adopted as final, with changes, the interim rule amending the Federal Acquisition...

75 FR 14057 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-40; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-23

...: Introduction, Small Entity Compliance Guide, Federal Awardee Performance and Integrity, Information System...; Federal Acquisition Circular 2005-40; Introduction AGENCIES: Department of Defense (DoD), General Services...

BioPlex Display: An Interactive Suite for Large-Scale AP-MS Protein-Protein Interaction Data.

PubMed

Schweppe, Devin K; Huttlin, Edward L; Harper, J Wade; Gygi, Steven P

2018-01-05

The development of large-scale data sets requires a new means to display and disseminate research studies to large audiences. Knowledge of protein-protein interaction (PPI) networks has become a principle interest of many groups within the field of proteomics. At the confluence of technologies, such as cross-linking mass spectrometry, yeast two-hybrid, protein cofractionation, and affinity purification mass spectrometry (AP-MS), detection of PPIs can uncover novel biological inferences at a high-throughput. Thus new platforms to provide community access to large data sets are necessary. To this end, we have developed a web application that enables exploration and dissemination of the growing BioPlex interaction network. BioPlex is a large-scale interactome data set based on AP-MS of baits from the human ORFeome. The latest BioPlex data set release (BioPlex 2.0) contains 56 553 interactions from 5891 AP-MS experiments. To improve community access to this vast compendium of interactions, we developed BioPlex Display, which integrates individual protein querying, access to empirical data, and on-the-fly annotation of networks within an easy-to-use and mobile web application. BioPlex Display enables rapid acquisition of data from BioPlex and development of hypotheses based on protein interactions.

48 CFR 352.215-1 - Instructions to offerors-competitive acquisition.

Code of Federal Regulations, 2010 CFR

2010-10-01

...-competitive acquisition. 352.215-1 Section 352.215-1 Federal Acquisition Regulations System HEALTH AND HUMAN....215-1 Instructions to offerors—competitive acquisition. As prescribed in 315.209, the Contracting... Offerors—Competitive Acquisition: (e) Restriction on disclosure and use of data. (1) The proposal submitted...

Defense Acquisition Workforce: The Air Force Needs to Evaluate Changes in Funding for Civilians Engaged in Space Acquisition

DTIC Science & Technology

2013-07-01

1) revitalize the acquisition workforce; (2) improve the requirements generation process; (3) instill budget and financial DOD Acquisition...with our four recommended actions (see app . I). In concurring with our recommendations, DOD stated that the Air Force will evaluate the pilot program

Buffer Gas Acquisition and Storage

NASA Technical Reports Server (NTRS)

Parrish, Clyde F.; Lueck, Dale E.; Jennings, Paul A.; Callahan, Richard A.; Delgado, H. (Technical Monitor)

2001-01-01

The acquisition and storage of buffer gases (primarily argon and nitrogen) from the Mars atmosphere provides a valuable resource for blanketing and pressurizing fuel tanks and as a buffer gas for breathing air for manned missions. During the acquisition of carbon dioxide (CO2), whether by sorption bed or cryo-freezer, the accompanying buffer gases build up in the carbon dioxide acquisition system, reduce the flow of CO2 to the bed, and lower system efficiency. It is this build up of buffer gases that provide a convenient source, which must be removed, for efficient capture Of CO2 Removal of this buffer gas barrier greatly improves the charging rate of the CO2 acquisition bed and, thereby, maintains the fuel production rates required for a successful mission. Consequently, the acquisition, purification, and storage of these buffer gases are important goals of ISRU plans. Purity of the buffer gases is a concern e.g., if the CO, freezer operates at 140 K, the composition of the inert gas would be approximately 21 percent CO2, 50 percent nitrogen, and 29 percent argon. Although there are several approaches that could be used, this effort focused on a hollow-fiber membrane (HFM) separation method. This study measured the permeation rates of CO2, nitrogen (ND, and argon (Ar) through a multiple-membrane system and the individual membranes from room temperature to 193K and 10 kpa to 300 kPa. Concentrations were measured with a gas chromatograph that used a thermoconductivity (TCD) detector with helium (He) as the carrier gas. The general trend as the temperature was lowered was for the membranes to become more selective, In addition, the relative permeation rates between the three gases changed with temperature. The end result was to provide design parameters that could be used to separate CO2 from N2 and Ar.

Heuristic-based information acquisition and decision making among pilots.

PubMed

Wiggins, Mark W; Bollwerk, Sandra

2006-01-01

This research was designed to examine the impact of heuristic-based approaches to the acquisition of task-related information on the selection of an optimal alternative during simulated in-flight decision making. The work integrated features of naturalistic and normative decision making and strategies of information acquisition within a computer-based, decision support framework. The study comprised two phases, the first of which involved familiarizing pilots with three different heuristic-based strategies of information acquisition: frequency, elimination by aspects, and majority of confirming decisions. The second stage enabled participants to choose one of the three strategies of information acquisition to resolve a fourth (choice) scenario. The results indicated that task-oriented experience, rather than the information acquisition strategies, predicted the selection of the optimal alternative. It was also evident that of the three strategies available, the elimination by aspects information acquisition strategy was preferred by most participants. It was concluded that task-oriented experience, rather than the process of information acquisition, predicted task accuracy during the decision-making task. It was also concluded that pilots have a preference for one particular approach to information acquisition. Applications of outcomes of this research include the development of decision support systems that adapt to the information-processing capabilities and preferences of users.

Data Acquisition and Real-Time Systems.

ERIC Educational Resources Information Center

Lawrence, D. E., Ed.; Fenwick, P. M., Ed.

The first group of papers starts with a tutorial paper which surveys the methods used in data acquisition systems. Other papers in this group describe: (1) some problems involved in the computer acquisition of high-speed randomly-occurring data and the protection of this data from accidental corruption, (2) an input/output bus to allow an IBM…

Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins.

PubMed

Gopinath, T; Veglia, Gianluigi

2018-01-01

Conventional NMR pulse sequences record one spectrum per experiment, while spending most of the time waiting for the spin system to return to the equilibrium. As a result, a full set of multidimensional NMR experiments for biological macromolecules may take up to several months to complete. Here, we present a practical guide for setting up a new class of MAS solid-state NMR experiments (POE or polarization optimized experiments) that enable the simultaneous acquisition of multiple spectra of proteins, accelerating data acquisition. POE exploit the long-lived 15 N polarization of isotopically labeled proteins and enable one to obtain up to eight spectra, by concatenating classical NMR pulse sequences. This new strategy propels data throughput of solid-state NMR spectroscopy of fibers, microcrystalline preparations, as well as membrane proteins.

Hospital Acquisitions Before Healthcare Reform.

PubMed

McCue, Michael J; Thompson, Jon M; Kim, Tae Hyun

2015-01-01

The hospital industry has experienced increased consolidation in the past 20 years. Since 2010, in particular, there has been a large rise in the number of hospital acquisitions, and observers have suggested this is due in part to the expected impact of federal healthcare reform legislation. This article reports on a study undertaken to identify the market, management, and financial factors affecting acute care, community hospitals acquired between 2010 and 2012. We identified 77 such hospitals and compared them to other acute care facilities. To assess how different factors were associated with acquisitions, the study used multiple logistic regressions whereby market factors were included first, followed by management and financial factors. Study findings show that acquired hospitals were located in markets with lower rates of preferred provider organization (PPO) penetration compared with nonacquired hospitals. Occupancy rate was found to be inversely related to acquisition rate; however, case-mix index was significantly and positively related to a hospital's being acquired. Financial factors negatively associated with a hospital's being acquired included age of plant and cash flow margin. In contrast to the findings from earlier studies of hospital acquisitions, our results showed that acquired hospitals possessed newer assets. However, similar to the findings of other studies, the cash flow margin of acquired hospitals was lower than that of nonacquired facilities.

48 CFR 939.7002 - Contractor acquisition of information technology.

Code of Federal Regulations, 2013 CFR

2013-10-01

... information technology. 939.7002 Section 939.7002 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SPECIAL CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY Implementing DOE Policies and Procedures 939.7002 Contractor acquisition of information technology. (a) Management and operating (M&O...

48 CFR 939.7002 - Contractor acquisition of information technology.

Code of Federal Regulations, 2014 CFR

2014-10-01

... information technology. 939.7002 Section 939.7002 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SPECIAL CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY Implementing DOE Policies and Procedures 939.7002 Contractor acquisition of information technology. (a) Management and operating (M&O...

48 CFR 939.7002 - Contractor acquisition of information technology.

Code of Federal Regulations, 2011 CFR

2011-10-01

... information technology. 939.7002 Section 939.7002 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SPECIAL CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY Implementing DOE Policies and Procedures 939.7002 Contractor acquisition of information technology. (a) Management and operating (M&O...

48 CFR 939.7002 - Contractor acquisition of information technology.

Code of Federal Regulations, 2012 CFR

2012-10-01

... information technology. 939.7002 Section 939.7002 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SPECIAL CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY Implementing DOE Policies and Procedures 939.7002 Contractor acquisition of information technology. (a) Management and operating (M&O...

48 CFR 939.7002 - Contractor acquisition of information technology.

Code of Federal Regulations, 2010 CFR

2010-10-01

... information technology. 939.7002 Section 939.7002 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SPECIAL CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY Implementing DOE Policies and Procedures 939.7002 Contractor acquisition of information technology. (a) Management and operating (M&O...

48 CFR 1.102-4 - Role of the acquisition team.

Code of Federal Regulations, 2011 CFR

2011-10-01

... team. 1.102-4 Section 1.102-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... acquisition team. (a) Government members of the Team must be empowered to make acquisition decisions within...) The Team must be prepared to perform the functions and duties assigned. The Government is committed to...

48 CFR 1.102-4 - Role of the acquisition team.

Code of Federal Regulations, 2012 CFR

2012-10-01

... team. 1.102-4 Section 1.102-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... acquisition team. (a) Government members of the Team must be empowered to make acquisition decisions within...) The Team must be prepared to perform the functions and duties assigned. The Government is committed to...

48 CFR 1.102-4 - Role of the acquisition team.

Code of Federal Regulations, 2013 CFR

2013-10-01

... team. 1.102-4 Section 1.102-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... acquisition team. (a) Government members of the Team must be empowered to make acquisition decisions within...) The Team must be prepared to perform the functions and duties assigned. The Government is committed to...

48 CFR 1.102-4 - Role of the acquisition team.

Code of Federal Regulations, 2010 CFR

2010-10-01

... team. 1.102-4 Section 1.102-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... acquisition team. (a) Government members of the Team must be empowered to make acquisition decisions within...) The Team must be prepared to perform the functions and duties assigned. The Government is committed to...

48 CFR 1.102-4 - Role of the acquisition team.

Code of Federal Regulations, 2014 CFR

2014-10-01

... team. 1.102-4 Section 1.102-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... acquisition team. (a) Government members of the Team must be empowered to make acquisition decisions within...) The Team must be prepared to perform the functions and duties assigned. The Government is committed to...

Handgun Acquisitions in California After Two Mass Shootings.

PubMed

Studdert, David M; Zhang, Yifan; Rodden, Jonathan A; Hyndman, Rob J; Wintemute, Garen J

2017-05-16

Mass shootings are common in the United States. They are the most visible form of firearm violence. Their effect on personal decisions to purchase firearms is not well-understood. To determine changes in handgun acquisition patterns after the mass shootings in Newtown, Connecticut, in 2012 and San Bernardino, California, in 2015. Time-series analysis using seasonal autoregressive integrated moving-average (SARIMA) models. California. Adults who acquired handguns between 2007 and 2016. Excess handgun acquisitions (defined as the difference between actual and expected acquisitions) in the 6-week and 12-week periods after each shooting, overall and within subgroups of acquirers. In the 6 weeks after the Newtown and San Bernardino shootings, there were 25 705 (95% prediction interval, 17 411 to 32 788) and 27 413 (prediction interval, 15 188 to 37 734) excess acquisitions, respectively, representing increases of 53% (95% CI, 30% to 80%) and 41% (CI, 19% to 68%) over expected volume. Large increases in acquisitions occurred among white and Hispanic persons, but not among black persons, and among persons with no record of having previously acquired a handgun. After the San Bernardino shootings, acquisition rates increased by 85% among residents of that city and adjacent neighborhoods, compared with 35% elsewhere in California. The data relate to handguns in 1 state. The statistical analysis cannot establish causality. Large increases in handgun acquisitions occurred after these 2 mass shootings. The spikes were short-lived and accounted for less than 10% of annual handgun acquisitions statewide. Further research should examine whether repeated shocks of this kind lead to substantial increases in the prevalence of firearm ownership. None.

Acquisition management of the Global Transportation Network

DOT National Transportation Integrated Search

2001-08-02

This report discusses the acquisition management of the Global transportation Network by the U.S. Transportation Command. This report is one in a series of audit reports addressing DoD acquisition management of information technology systems. The Glo...

AZ-101 Mixer Pump Demonstration Data Acquisition System and Gamma Cart Data Acquisition Control System Software Configuration Management Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

WHITE, D.A.

1999-12-29

This Software Configuration Management Plan (SCMP) provides the instructions for change control of the AZ1101 Mixer Pump Demonstration Data Acquisition System (DAS) and the Sludge Mobilization Cart (Gamma Cart) Data Acquisition and Control System (DACS).

Cross-linguistic patterns in the acquisition of quantifiers

PubMed Central

Cummins, Chris; Gavarró, Anna; Kuvač Kraljević, Jelena; Hrzica, Gordana; Grohmann, Kleanthes K.; Skordi, Athina; Jensen de López, Kristine; Sundahl, Lone; van Hout, Angeliek; Hollebrandse, Bart; Overweg, Jessica; Faber, Myrthe; van Koert, Margreet; Smith, Nafsika; Vija, Maigi; Zupping, Sirli; Kunnari, Sari; Morisseau, Tiffany; Rusieshvili, Manana; Yatsushiro, Kazuko; Fengler, Anja; Varlokosta, Spyridoula; Konstantzou, Katerina; Farby, Shira; Guasti, Maria Teresa; Vernice, Mirta; Okabe, Reiko; Isobe, Miwa; Crosthwaite, Peter; Hong, Yoonjee; Balčiūnienė, Ingrida; Ahmad Nizar, Yanti Marina; Grech, Helen; Gatt, Daniela; Cheong, Win Nee; Asbjørnsen, Arve; Torkildsen, Janne von Koss; Haman, Ewa; Miękisz, Aneta; Gagarina, Natalia; Puzanova, Julia; Anđelković, Darinka; Savić, Maja; Jošić, Smiljana; Slančová, Daniela; Kapalková, Svetlana; Barberán, Tania; Özge, Duygu; Hassan, Saima; Chan, Cecilia Yuet Hung; Okubo, Tomoya; van der Lely, Heather; Sauerland, Uli; Noveck, Ira

2016-01-01

Learners of most languages are faced with the task of acquiring words to talk about number and quantity. Much is known about the order of acquisition of number words as well as the cognitive and perceptual systems and cultural practices that shape it. Substantially less is known about the acquisition of quantifiers. Here, we consider the extent to which systems and practices that support number word acquisition can be applied to quantifier acquisition and conclude that the two domains are largely distinct in this respect. Consequently, we hypothesize that the acquisition of quantifiers is constrained by a set of factors related to each quantifier’s specific meaning. We investigate competence with the expressions for “all,” “none,” “some,” “some…not,” and “most” in 31 languages, representing 11 language types, by testing 768 5-y-old children and 536 adults. We found a cross-linguistically similar order of acquisition of quantifiers, explicable in terms of four factors relating to their meaning and use. In addition, exploratory analyses reveal that language- and learner-specific factors, such as negative concord and gender, are significant predictors of variation. PMID:27482119

Developing Program Management Leadership for Acquisition Reform

DTIC Science & Technology

2011-04-30

mêçÅÉÉÇáåÖë= çÑ=íÜÉ= bfdeqe=^kkr^i=^`nrfpfqflk== obpb^o`e=pvjmlpfrj== qeropa^v=pbppflkp== slirjb ff Developing Program Management Leadership for...4. TITLE AND SUBTITLE Developing Program Management Leadership for Acquisition Reform 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT...Developing Program  Management   Leadership   for Acquisition Reform    The 8th Annual Acquisition Research Symposium Panel #20: Investing in People

Reading Acquisition and Beyond: Decoding Includes Cognition.

ERIC Educational Resources Information Center

Perfetti, Charles A.

1984-01-01

Focuses on (1) the acquisition and use of word representations and (2) the acquisition of the alphabetic code. Urges that instruction provide conditions to promote the learning of three types of representation--word forms, letter patterns, and mapping. (RDN)

Defense Acquisition Review Journal. Volume 14, Number 2

DTIC Science & Technology

2007-09-01

2007 Vol. 14 No. 2 Learn. Perform. Succeed. Professionalism in the Acquisition Contracting Workforce Have We Gone too Far? John Krieger Contracting...acQuiSition contractinG WorKforce: HAVE WE GONE TOO FAR? John Krieger To professionalize the acquisition contracting workforce, the Department of Defense...featured author for this edition is Professor John Krieger , the Director of the Contracting Center of the Defense Acquisition University’s

Acquisitions and Collection Development Automation: Future Directions.

ERIC Educational Resources Information Center

Aveney, Brian; Heinemann, Luba

1983-01-01

This paper explores features of automated acquisitions systems now implemented and discusses features that might be implemented in the next few years. Distributed acquisitions, ordering, receiving, reconciliation, management reporting, and collection development (selection of current materials, retrospective purchasing, and comparative…

12 CFR 574.3 - Acquisition of control of savings associations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... authorize such an acquisition by language to that effect and not merely by implication. [54 FR 49690, Nov... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Acquisition of control of savings associations... ACQUISITION OF CONTROL OF SAVINGS ASSOCIATIONS § 574.3 Acquisition of control of savings associations. (a...

76 FR 14566 - Federal Acquisition Regulation; Socioeconomic Program Parity

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-16

... 9000-AL88 Federal Acquisition Regulation; Socioeconomic Program Parity AGENCY: Department of Defense... Acquisition Regulation (FAR) to implement section 1347 of the ``Small Business Jobs Act of 2010.'' Section... acquisition will be restricted to small businesses participating in the 8(a), HUBZone, or service-disabled...

48 CFR 4.705-3 - Acquisition and supply records.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisition and supply... GENERAL ADMINISTRATIVE MATTERS Contractor Records Retention 4.705-3 Acquisition and supply records. (a) Store requisitions for materials, supplies, equipment, and services: Retain 2 years. (b) Work orders for...

Acquisition of Scientific Literature in Developing Countries. 2: Malaysia.

ERIC Educational Resources Information Center

Taib, Rosna

1989-01-01

Describes the acquisition of scientific literature by academic libraries in Malaysia. The discussion covers the impact of government policies, library acquisition policies, the selection process, acquisition of special materials, the role of gifts and exchanges, and problems with customs clearance and censorship. Progress in cooperative…

A Research on Second Language Acquisition and College English Teaching

ERIC Educational Resources Information Center

Li, Changyu

2009-01-01

It was in the 1970s that American linguist S.D. Krashen created the theory of "language acquisition". The theories on second language acquisition were proposed based on the study on the second language acquisition process and its rules. Here, the second language acquisition process refers to the process in which a learner with the…

Selecting Senior Acquisition Officials: Assessing the Current Processes and Practices for Recruiting, Confirming, and Retaining Senior Officials in the Acquisition Workforce

DTIC Science & Technology

2016-04-21

Selecting Senior Acquisition Officials Assessing the Current Processes and Practices for Recruiting, Confirming, and Retaining Senior Officials...Task Group 2 Terms of Reference (TOR)  Selection of Senior Officials in the Acquisition Workforce – Consider ethics rules, congressional committee... Senior Acquisition positions – Re-validate the conflicts of interest and risk mitigation rules “[T]he committee directs the Chair of the Defense Business

Advanced Data Acquisition Systems

NASA Technical Reports Server (NTRS)

Perotti, J.

2003-01-01

Current and future requirements of the aerospace sensors and transducers field make it necessary for the design and development of new data acquisition devices and instrumentation systems. New designs are sought to incorporate self-health, self-calibrating, self-repair capabilities, allowing greater measurement reliability and extended calibration cycles. With the addition of power management schemes, state-of-the-art data acquisition systems allow data to be processed and presented to the users with increased efficiency and accuracy. The design architecture presented in this paper displays an innovative approach to data acquisition systems. The design incorporates: electronic health self-check, device/system self-calibration, electronics and function self-repair, failure detection and prediction, and power management (reduced power consumption). These requirements are driven by the aerospace industry need to reduce operations and maintenance costs, to accelerate processing time and to provide reliable hardware with minimum costs. The project's design architecture incorporates some commercially available components identified during the market research investigation like: Field Programmable Gate Arrays (FPGA) Programmable Analog Integrated Circuits (PAC IC) and Field Programmable Analog Arrays (FPAA); Digital Signal Processing (DSP) electronic/system control and investigation of specific characteristics found in technologies like: Electronic Component Mean Time Between Failure (MTBF); and Radiation Hardened Component Availability. There are three main sections discussed in the design architecture presented in this document. They are the following: (a) Analog Signal Module Section, (b) Digital Signal/Control Module Section and (c) Power Management Module Section. These sections are discussed in detail in the following pages. This approach to data acquisition systems has resulted in the assignment of patent rights to Kennedy Space Center under U.S. patent # 6

48 CFR 218.170 - Additional acquisition flexibilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES EMERGENCY ACQUISITIONS Available... vessels. The contracting officer, without soliciting offers, may issue a written job order for emergency..., Restrictions on food, clothing, fabrics, specialty metals, and hand or measuring tools: (1) Acquisitions at or...

18 CFR 1306.2 - Uniform real property acquisition policy.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Uniform real property... RELOCATION ASSISTANCE AND REAL PROPERTY ACQUISITION POLICIES Regulations and Procedures § 1306.2 Uniform real property acquisition policy. (a) Before negotiations are initiated for acquisition of real property, the...

18 CFR 1306.2 - Uniform real property acquisition policy.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Uniform real property... RELOCATION ASSISTANCE AND REAL PROPERTY ACQUISITION POLICIES Regulations and Procedures § 1306.2 Uniform real property acquisition policy. (a) Before negotiations are initiated for acquisition of real property, the...

48 CFR 945.570-2 - Acquisition of motor vehicles.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Acquisition of motor... Acquisition of motor vehicles. (a) The GSA Interagency Fleet Management System (GSA-IFMS) is the first source of supply for providing motor vehicles to contractors; however, contracting officer approval is...

48 CFR 945.570-2 - Acquisition of motor vehicles.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Acquisition of motor... Acquisition of motor vehicles. (a) The GSA Interagency Fleet Management System (GSA-IFMS) is the first source of supply for providing motor vehicles to contractors; however, contracting officer approval is...

Impacts of Space System Acquisition on National Security

DTIC Science & Technology

2012-12-01

and observation have indicated that differences in the theory and practice of acquisition, conflicts in values among the acquisition community, and...budgets, requirements and acquisition. The “DAPA Report January 2006,” states, “In theory , new weapon systems are delivered as the result of the... stockholder confidence.86 Additionally, the “DAPA Executive Report December 2005” states: “While each of these sets of values is legitimate, pursuing them

My 65 years in protein chemistry.

PubMed

Scheraga, Harold A

2015-05-01

This is a tour of a physical chemist through 65 years of protein chemistry from the time when emphasis was placed on the determination of the size and shape of the protein molecule as a colloidal particle, with an early breakthrough by James Sumner, followed by Linus Pauling and Fred Sanger, that a protein was a real molecule, albeit a macromolecule. It deals with the recognition of the nature and importance of hydrogen bonds and hydrophobic interactions in determining the structure, properties, and biological function of proteins until the present acquisition of an understanding of the structure, thermodynamics, and folding pathways from a linear array of amino acids to a biological entity. Along the way, with a combination of experiment and theoretical interpretation, a mechanism was elucidated for the thrombin-induced conversion of fibrinogen to a fibrin blood clot and for the oxidative-folding pathways of ribonuclease A. Before the atomic structure of a protein molecule was determined by x-ray diffraction or nuclear magnetic resonance spectroscopy, experimental studies of the fundamental interactions underlying protein structure led to several distance constraints which motivated the theoretical approach to determine protein structure, and culminated in the Empirical Conformational Energy Program for Peptides (ECEPP), an all-atom force field, with which the structures of fibrous collagen-like proteins and the 46-residue globular staphylococcal protein A were determined. To undertake the study of larger globular proteins, a physics-based coarse-grained UNited-RESidue (UNRES) force field was developed, and applied to the protein-folding problem in terms of structure, thermodynamics, dynamics, and folding pathways. Initially, single-chain and, ultimately, multiple-chain proteins were examined, and the methodology was extended to protein-protein interactions and to nucleic acids and to protein-nucleic acid interactions. The ultimate results led to an understanding

Combining factual and heuristic knowledge in knowledge acquisition

NASA Technical Reports Server (NTRS)

Gomez, Fernando; Hull, Richard; Karr, Clark; Hosken, Bruce; Verhagen, William

1992-01-01

A knowledge acquisition technique that combines heuristic and factual knowledge represented as two hierarchies is described. These ideas were applied to the construction of a knowledge acquisition interface to the Expert System Analyst (OPERA). The goal of OPERA is to improve the operations support of the computer network in the space shuttle launch processing system. The knowledge acquisition bottleneck lies in gathering knowledge from human experts and transferring it to OPERA. OPERA's knowledge acquisition problem is approached as a classification problem-solving task, combining this approach with the use of factual knowledge about the domain. The interface was implemented in a Symbolics workstation making heavy use of windows, pull-down menus, and other user-friendly devices.

7 CFR 1735.62 - Approval of acquisitions and mergers.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false Approval of acquisitions and mergers. 1735.62 Section 1735.62 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE... Mortgage Controls on Acquisitions and Mergers § 1735.62 Approval of acquisitions and mergers. (a) If a...

76 FR 76899 - Federal Acquisition Regulation; Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-09

...-AM11 Federal Acquisition Regulation; Correction AGENCIES: Department of Defense (DoD), General Services... Regulation (FAR) to revise the definitions of ``Caribbean Basin country'' and ``designated country'' due to... Register of Wednesday, November 2, 2011 (76 FR 68039). The rule amended the Federal Acquisition Regulation...

Assessment of Accelerated Acquisition of Defense Programs

DTIC Science & Technology

2016-09-01

I N S T I T U T E F O R D E F E N S E A N A L Y S E S Assessment of Accelerated Acquisition of Defense Programs Richard H. Van Atta R. Royce...Defense for Acquisition . The views, opinions, and findings should not be construed as representing the official position of either the Department of...S T I T U T E F O R D E F E N S E A N A L Y S E S IDA Paper P-8161 Assessment of Accelerated Acquisition of Defense Programs Richard H. Van Atta

TFTR diagnostic control and data acquisition system

NASA Astrophysics Data System (ADS)

Sauthoff, N. R.; Daniels, R. E.

1985-05-01

General computerized control and data-handling support for TFTR diagnostics is presented within the context of the Central Instrumentation, Control and Data Acquisition (CICADA) System. Procedures, hardware, the interactive man-machine interface, event-driven task scheduling, system-wide arming and data acquisition, and a hierarchical data base of raw data and results are described. Similarities in data structures involved in control, monitoring, and data acquisition afford a simplification of the system functions, based on ``groups'' of devices. Emphases and optimizations appropriate for fusion diagnostic system designs are provided. An off-line data reduction computer system is under development.

TFTR diagnostic control and data acquisition system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sauthoff, N.R.; Daniels, R.E.; PPL Computer Division

1985-05-01

General computerized control and data-handling support for TFTR diagnostics is presented within the context of the Central Instrumentation, Control and Data Acquisition (CICADA) System. Procedures, hardware, the interactive man--machine interface, event-driven task scheduling, system-wide arming and data acquisition, and a hierarchical data base of raw data and results are described. Similarities in data structures involved in control, monitoring, and data acquisition afford a simplification of the system functions, based on ''groups'' of devices. Emphases and optimizations appropriate for fusion diagnostic system designs are provided. An off-line data reduction computer system is under development.

Complexity in language acquisition.

PubMed

Clark, Alexander; Lappin, Shalom

2013-01-01

Learning theory has frequently been applied to language acquisition, but discussion has largely focused on information theoretic problems-in particular on the absence of direct negative evidence. Such arguments typically neglect the probabilistic nature of cognition and learning in general. We argue first that these arguments, and analyses based on them, suffer from a major flaw: they systematically conflate the hypothesis class and the learnable concept class. As a result, they do not allow one to draw significant conclusions about the learner. Second, we claim that the real problem for language learning is the computational complexity of constructing a hypothesis from input data. Studying this problem allows for a more direct approach to the object of study--the language acquisition device-rather than the learnable class of languages, which is epiphenomenal and possibly hard to characterize. The learnability results informed by complexity studies are much more insightful. They strongly suggest that target grammars need to be objective, in the sense that the primitive elements of these grammars are based on objectively definable properties of the language itself. These considerations support the view that language acquisition proceeds primarily through data-driven learning of some form. Copyright © 2013 Cognitive Science Society, Inc.

MRSA acquisition in an intensive care unit.

PubMed

Dancer, Stephanie J; Coyne, Michael; Speekenbrink, A; Samavedam, Sam; Kennedy, Julie; Wallace, Peter G M

2006-02-01

This paper describes a retrospective investigation of methicillin-resistant Staphylococcus aureus (MRSA) acquisition in an 8-bed intensive care unit (ICU) over a 5-month period. Clinical and microbiologic data were collected from the ICU, including MRSA detection dates, patient dependency scores, standardized environmental screening data, weekly bed occupancies, number of admissions, and nurse staffing levels. MRSA acquisition weeks were defined as weeks during which initial delivery of MRSA occurred before sampling and laboratory confirmation. Weekly workloads were plotted against staffing levels and modelled against MRSA acquisition weeks and hygiene failures. Of 174 patients admitted into the ICU, 28 (16%) were found to have MRSA; 12 of these (7%) acquired MRSA on the ICU within 7 of the 23 weeks studied. Six of these 7 weeks were associated with a deficit of trained nurses during the day and 5 with hygiene failures (data unavailable for 2). Pulsed-field gel electrophoresis (PFGE) profiles demonstrated relationships between staphylococci from staff hands, hand-touch sites, and patients' blood. MRSA acquisition in the ICU was temporally associated with reduced numbers of trained nurses and hygiene failures predominantly involving hand-touch sites. Epidemiologic analysis suggested that patient acquisitions were 7 times more likely to occur during periods of nurse understaffing.

Thermal Optimization of Growth and Quality in Protein Crystals

NASA Technical Reports Server (NTRS)

Wiencek, John M.

1996-01-01

Experimental evidence suggests that larger and higher quality crystals can be attained in the microgravity of space; however, the effect of growth rate on protein crystal quality is not well documented. This research is the first step towards providing strategies to grow crystals under constant rates of growth. Controlling growth rates at a constant value allows for direct one-to-one comparison of results obtained in microgravity and on earth. The overall goal of the project was to control supersaturation at a constant value during protein crystal growth by varying temperature in a predetermined manner. Applying appropriate theory requires knowledge of specific physicochemical properties of the protein solution including the effect of supersaturation on growth rates and the effect of temperature on protein solubility. Such measurements typically require gram quantities of protein and many months of data acquisition. A second goal of the project applied microcalorimetry for the rapid determination of these physicochemical properties using a minimum amount of protein. These two goals were successfully implemented on hen egg-white lysozyme. Results of these studies are described in the attached reprints.

34 CFR 75.533 - Acquisition of real property; construction.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 1 2010-07-01 2010-07-01 false Acquisition of real property; construction. 75.533... Conditions Must Be Met by a Grantee? Allowable Costs § 75.533 Acquisition of real property; construction. No grantee may use its grant for acquisition of real property or for construction unless specifically...

48 CFR 225.7702-1 - Acquisition of small arms.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Acquisition of small arms... Operations in Afghanistan 225.7702-1 Acquisition of small arms. (a) Except as provided in paragraph (b) of this section, when acquiring small arms for assistance to the Army of Afghanistan, the Afghani Police...

48 CFR 225.7702-1 - Acquisition of small arms.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Acquisition of small arms... Operations in Afghanistan 225.7702-1 Acquisition of small arms. (a) Except as provided in paragraph (b) of this section, when acquiring small arms for assistance to the Army of Afghanistan, the Afghani Police...

75 FR 51884 - Department of Education Acquisition Regulation

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-23

...The Secretary proposes to reissue the Department of Education Acquisition Regulation (EDAR) in order to update it to accurately implement the current Federal Acquisition Regulation (FAR) and Department policies.

23 CFR 710.603 - Direct Federal acquisition.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 23 Highways 1 2012-04-01 2012-04-01 false Direct Federal acquisition. 710.603 Section 710.603 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT RIGHT-OF-WAY AND REAL ESTATE Federal Assistance Programs § 710.603 Direct Federal acquisition. (a) The...

32 CFR 644.5 - Mineral acquisition practices.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 4 2011-07-01 2011-07-01 false Mineral acquisition practices. 644.5 Section 644.5 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Project Planning Civil Works § 644.5 Mineral acquisition practices. (a...

Studies in First and Second Language Acquisition.

ERIC Educational Resources Information Center

Eckman, Fred R., Ed.; Hastings, Ashley J., Ed.

Papers presented at a 1977 symposium on language acquisition held at the University of Wisconsin/Milwaukee are included. Contents are as follows: "Assumptions, Methods and Goals in Language Acquisition Research" (Sheldon); "The Mother as LAD: Interaction between Order and Frequency of Parental Input and Child Production"…

New Dimensions in Second Language Acquisition Research.

ERIC Educational Resources Information Center

Andersen, Roger W., Ed.

The following papers are included: (1) "Some Common Goals for Second and First Language Acquisition Research" by Kenji Hakuta; (2) "Research on the Measurement of Affective Variables: Some Remaining Questions" by John W. Oller, Jr.; (3) "The Effects of Neurological Age on Nonprimary Language Acquisition" by Thomas…

32 CFR 552.37 - Acquisition by Chief of Engineers.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 3 2011-07-01 2009-07-01 true Acquisition by Chief of Engineers. 552.37 Section... Interest Therein § 552.37 Acquisition by Chief of Engineers. (a) Statutory authority. The Chief of Engineers, under the direction of the Secretary of the Army, is charged with the acquisition of all real...

32 CFR 552.37 - Acquisition by Chief of Engineers.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 3 2012-07-01 2009-07-01 true Acquisition by Chief of Engineers. 552.37 Section... Interest Therein § 552.37 Acquisition by Chief of Engineers. (a) Statutory authority. The Chief of Engineers, under the direction of the Secretary of the Army, is charged with the acquisition of all real...

32 CFR 552.37 - Acquisition by Chief of Engineers.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 3 2014-07-01 2014-07-01 false Acquisition by Chief of Engineers. 552.37... Interest Therein § 552.37 Acquisition by Chief of Engineers. (a) Statutory authority. The Chief of Engineers, under the direction of the Secretary of the Army, is charged with the acquisition of all real...

32 CFR 552.37 - Acquisition by Chief of Engineers.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 3 2013-07-01 2013-07-01 false Acquisition by Chief of Engineers. 552.37... Interest Therein § 552.37 Acquisition by Chief of Engineers. (a) Statutory authority. The Chief of Engineers, under the direction of the Secretary of the Army, is charged with the acquisition of all real...

32 CFR 552.37 - Acquisition by Chief of Engineers.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Acquisition by Chief of Engineers. 552.37 Section... Interest Therein § 552.37 Acquisition by Chief of Engineers. (a) Statutory authority. The Chief of Engineers, under the direction of the Secretary of the Army, is charged with the acquisition of all real...

75 FR 77727 - Federal Acquisition Regulation; HUBZone Program Revisions

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-13

... (NASA). ACTION: Final rule. SUMMARY: The Civilian Agency Acquisition Council and the Defense Acquisition... Case 2006-005. SUPPLEMENTARY INFORMATION: A. Background DoD, GSA, and NASA published a proposed rule in..., Acquisition Policy Division. 0 Therefore, DoD, GSA, and NASA amend 48 CFR parts 2, 19, 33, and 52 as set forth...

29 CFR 2550.408e - Statutory exemption for acquisition or sale of qualifying employer securities and for acquisition...

Code of Federal Regulations, 2010 CFR

2010-07-01

... or sale by a plan of qualifying employer securities (as defined in section 407(d)(5) of the Act), or any acquisition, sale or lease by a plan of qualifying employer real property (as defined in section... 29 Labor 9 2010-07-01 2010-07-01 false Statutory exemption for acquisition or sale of qualifying...

Preparation and characterization of hydroxyapatite/sodium alginate biocomposites for bone implant application

NASA Astrophysics Data System (ADS)

Kanasan, Nanthini; Adzila, Sharifah; Suid, Mohd Syafiq; Gurubaran, P.

2016-07-01

In biomedical fields, synthetic scaffolds are being improved by using the ceramics, polymers and composites materials to avoid the limitations of allograft. Ceramic-polymer composites are appearing to be the most successful bone graft substitute in human body. The natural bones itself are well-known as composite of collagen and hydroxyapatite. In this research, precipitation method was used to synthesis hydroxyapatite (HA)/sodium alginate (SA) in various parameters. This paper describes the hydroxyapatite/sodium alginate biocomposite which suitable for use in bone defects or regeneration of bone through the characterizations which include FTIR, FESEM, EDS and DTA. In FTIR, the characteristi peaks of PO4-3 and OH- groups which corresponding to hydroxyapatite are existed in the mixing powders. The needle-size particle of hydroxyapatite/ alginate (HA/SA) are observed in FESEM in the range of 15.8nm-38.2nm.EDS confirmed the existence of HA/SA composition in the mixing powders. There is an endothermic peak which corresponds to the dehydration and the loss of physically adsorbed water molecules of the hydroxyapatite (HA)/sodium alginate (SA) powder which are described in DTA.

Probing Protein Structure in Vivo with FRET

PubMed Central

Davis, Trisha; Muller, Eric

2012-01-01

Fluorescence resonance energy transfer (FRET) is widely used to construct probes for cellular activities and to complement two-hybrid results that predict protein-protein interactions. The Yeast Resource Center promotes an underutilized potential of FRET as an in vivo tool to position proteins within low resolution structures derived from electron microscopy. The success of this approach using widefield microscopy depends upon the choice of filter sets, standardized image acquisition, a robust metric and controls matched to the structure under investigation. A comparison of various CFP and YFP filter combinations from Chroma and Semrock demonstrated the strength of the Chroma filters when coupled with our FRET metric, termed FretR. Coupling CFP and YFP to a selection of proteins of known structure allowed us to create a standard curve of FretR versus distance. How well other FRET metrics conform was also evaluated. Finally FretR was linked to an approximation of the efficiency of energy transfer. Together this feature set has allowed us to contribute to our understanding of the organization of the yeast spindle pole body, cohesin complex and gamma-tubulin complex.

Air Traffic Control: Immature Software Acquisition Processes Increase FAA System Acquisition Risks

DOT National Transportation Integrated Search

1997-03-01

The General Accounting Office (GAO) at the request of Congress reviewed (1) : the maturity of Federal Aviation Administration's (FAA's) Air Traffic Control : (ATC) modernization software acquisition processes, and (2) the steps/actions : FAA has unde...

Second Language Acquisition.

ERIC Educational Resources Information Center

McLaughlin, Barry; Harrington, Michael

1989-01-01

A distinction is drawn between representational and processing models of second-language acquisition. The first approach is derived primarily from linguistics, the second from psychology. Both fields, it is argued, need to collaborate more fully, overcoming disciplinary narrowness in order to achieve more fruitful research. (GLR)

My 65 years in protein chemistry

PubMed Central

Scheraga, Harold A.

2015-01-01

This is a tour of a physical chemist through 65 years of protein chemistry from the time when emphasis was placed on the determination of the size and shape of the protein molecule as a colloidal particle, with an early breakthrough by James Sumner, followed by Linus Pauling and Fred Sanger, that a protein was a real molecule, albeit a macromolecule. It deals with the recognition of the nature and importance of hydrogen bonds and hydrophobic interactions in determining the structure, properties, and biological function of proteins until the present acquisition of an understanding of the structure, thermodynamics, and folding pathways from a linear array of amino acids to a biological entity. Along the way, with a combination of experiment and theoretical interpretation, a mechanism was elucidated for the thrombin-induced conversion of fibrinogen to a fibrin blood clot and for the oxidative-folding pathways of ribonuclease A. Before the atomic structure of a protein molecule was determined by x-ray diffraction or nuclear magnetic resonance spectroscopy, experimental studies of the fundamental interactions underlying protein structure led to several distance constraints which motivated the theoretical approach to determine protein structure, and culminated in the Empirical Conformational Energy Program for Peptides (ECEPP), an all-atom force field, with which the structures of fibrous collagen-like proteins and the 46-residue globular staphylococcal protein A were determined. To undertake the study of larger globular proteins, a physics-based coarse-grained UNited-RESidue (UNRES) force field was developed, and applied to the protein-folding problem in terms of structure, thermodynamics, dynamics, and folding pathways. Initially, single-chain and, ultimately, multiple-chain proteins were examined, and the methodology was extended to protein–protein interactions and to nucleic acids and to protein–nucleic acid interactions. The ultimate results led to an

Front-End Electron Transfer Dissociation Coupled to a 21 Tesla FT-ICR Mass Spectrometer for Intact Protein Sequence Analysis

NASA Astrophysics Data System (ADS)

Weisbrod, Chad R.; Kaiser, Nathan K.; Syka, John E. P.; Early, Lee; Mullen, Christopher; Dunyach, Jean-Jacques; English, A. Michelle; Anderson, Lissa C.; Blakney, Greg T.; Shabanowitz, Jeffrey; Hendrickson, Christopher L.; Marshall, Alan G.; Hunt, Donald F.

2017-09-01

High resolution mass spectrometry is a key technology for in-depth protein characterization. High-field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) enables high-level interrogation of intact proteins in the most detail to date. However, an appropriate complement of fragmentation technologies must be paired with FTMS to provide comprehensive sequence coverage, as well as characterization of sequence variants, and post-translational modifications. Here we describe the integration of front-end electron transfer dissociation (FETD) with a custom-built 21 tesla FT-ICR mass spectrometer, which yields unprecedented sequence coverage for proteins ranging from 2.8 to 29 kDa, without the need for extensive spectral averaging (e.g., 60% sequence coverage for apo-myoglobin with four averaged acquisitions). The system is equipped with a multipole storage device separate from the ETD reaction device, which allows accumulation of multiple ETD fragment ion fills. Consequently, an optimally large product ion population is accumulated prior to transfer to the ICR cell for mass analysis, which improves mass spectral signal-to-noise ratio, dynamic range, and scan rate. We find a linear relationship between protein molecular weight and minimum number of ETD reaction fills to achieve optimum sequence coverage, thereby enabling more efficient use of instrument data acquisition time. Finally, real-time scaling of the number of ETD reactions fills during method-based acquisition is shown, and the implications for LC-MS/MS top-down analysis are discussed. [Figure not available: see fulltext.

Impact of Merger and Acquisition on University Performance.

PubMed

Mohamadi Bolbanabad, Amjad; Mosadeghrad, Ali Mohammad; Arab, Mohammad; Majdzadeh, Reza

2017-08-01

Merger and acquisitions (M&A) widen access to higher education, improve quality of teaching and research, and increase efficiency of higher education institutions. Three big medical universities in Iran merged in 2010. The purpose of this study was to highlight the impact of this acquisition on the performance of Tehran University of Medical Sciences. This qualitative study was conducted using semi-structured in-depth interviews with 60 participants from April 2015 to November 2015. Purposeful sampling was used to recruit participants. Data analysis was performed using MAXQDA software (V. 10). The reasons for acquisition of IUMS by TUMS were to enhance the position of TUMS worldwide, and to increase its efficiency. The acquisition improved the organization and management of the newly formed TUMS, enhanced its academic position and market recognition. However, the achieved benefits did not come without shortcomings and unanticipated consequences. The merger and acquisition in higher education has some advantages and disadvantages. A well planned and carefully implemented M&A gives cutting edge to the higher education institutes.

ϕX174 Procapsid Assembly: Effects of an Inhibitory External Scaffolding Protein and Resistant Coat Proteins In Vitro.

PubMed

Cherwa, James E; Tyson, Joshua; Bedwell, Gregory J; Brooke, Dewey; Edwards, Ashton G; Dokland, Terje; Prevelige, Peter E; Fane, Bentley A

2017-01-01

During ϕX174 morphogenesis, 240 copies of the external scaffolding protein D organize 12 pentameric assembly intermediates into procapsids, a reaction reconstituted in vitro In previous studies, ϕX174 strains resistant to exogenously expressed dominant lethal D genes were experimentally evolved. Resistance was achieved by the stepwise acquisition of coat protein mutations. Once resistance was established, a stimulatory D protein mutation that greatly increased strain fitness arose. In this study, in vitro biophysical and biochemical methods were utilized to elucidate the mechanistic details and evolutionary trade-offs created by the resistance mutations. The kinetics of procapsid formation was analyzed in vitro using wild-type, inhibitory, and experimentally evolved coat and scaffolding proteins. Our data suggest that viral fitness is correlated with in vitro assembly kinetics and demonstrate that in vivo experimental evolution can be analyzed within an in vitro biophysical context. Experimental evolution is an extremely valuable tool. Comparisons between ancestral and evolved genotypes suggest hypotheses regarding adaptive mechanisms. However, it is not always possible to rigorously test these hypotheses in vivo We applied in vitro biophysical and biochemical methods to elucidate the mechanistic details that allowed an experimentally evolved virus to become resistant to an antiviral protein and then evolve a productive use for that protein. Moreover, our results indicate that the respective roles of scaffolding and coat proteins may have been redistributed during the evolution of a two-scaffolding-protein system. In one-scaffolding-protein virus assembly systems, coat proteins promiscuously interact to form heterogeneous aberrant structures in the absence of scaffolding proteins. Thus, the scaffolding protein controls fidelity. During ϕX174 assembly, the external scaffolding protein acts like a coat protein, self-associating into large aberrant spherical

The Defense Acquisition Workforce Improvement Strategy. Appendix 1: DOD Strategic Human Capital Plan Update. The Defense Acquisition Workforce

DTIC Science & Technology

2010-04-01

Frank J . Anderson, Jr., April 5, 2010. A Message from the Under Secretary of Defense For Acquisition, Technology and Logistics The importance of...Defense agencies. The Deputy Secretary of Defense, the Honorable William J . Lynn III, recently emphasized that the acquisition workforce rebalancing...20 Asch B., Haider S., and Zizzimopoulos, J . (2003) The Effects of Workforce-Shaping Incentives

48 CFR 227.7105 - Contracts for the acquisition of existing works.

Code of Federal Regulations, 2010 CFR

2010-10-01

... COPYRIGHTS Rights in Technical Data 227.7105 Contracts for the acquisition of existing works. ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Contracts for the acquisition of existing works. 227.7105 Section 227.7105 Federal Acquisition Regulations System DEFENSE...

Data acquisition instruments: Psychopharmacology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hartley, D.S. III

This report contains the results of a Direct Assistance Project performed by Lockheed Martin Energy Systems, Inc., for Dr. K. O. Jobson. The purpose of the project was to perform preliminary analysis of the data acquisition instruments used in the field of psychiatry, with the goal of identifying commonalities of data and strategies for handling and using the data in the most advantageous fashion. Data acquisition instruments from 12 sources were provided by Dr. Jobson. Several commonalities were identified and a potentially useful data strategy is reported here. Analysis of the information collected for utility in performing diagnoses is recommended.more » In addition, further work is recommended to refine the commonalities into a directly useful computer systems structure.« less

48 CFR 3409.570 - Certification at or below the simplified acquisition threshold.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Certification at or below the simplified acquisition threshold. 3409.570 Section 3409.570 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION REGULATION COMPETITION AND ACQUISITION PLANNING CONTRACTOR QUALIFICATIONS Organizational and Consultant...

48 CFR 3409.570 - Certification at or below the simplified acquisition threshold.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 7 2011-10-01 2011-10-01 false Certification at or below the simplified acquisition threshold. 3409.570 Section 3409.570 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION REGULATION COMPETITION AND ACQUISITION PLANNING CONTRACTOR QUALIFICATIONS Organizational and Consultant...

48 CFR 3409.570 - Certification at or below the simplified acquisition threshold.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Certification at or below the simplified acquisition threshold. 3409.570 Section 3409.570 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION REGULATION COMPETITION AND ACQUISITION PLANNING CONTRACTOR QUALIFICATIONS Organizational and Consultant...

Acquisition of speech rhythm in first language.

PubMed

Polyanskaya, Leona; Ordin, Mikhail

2015-09-01

Analysis of English rhythm in speech produced by children and adults revealed that speech rhythm becomes increasingly more stress-timed as language acquisition progresses. Children reach the adult-like target by 11 to 12 years. The employed speech elicitation paradigm ensured that the sentences produced by adults and children at different ages were comparable in terms of lexical content, segmental composition, and phonotactic complexity. Detected differences between child and adult rhythm and between rhythm in child speech at various ages cannot be attributed to acquisition of phonotactic language features or vocabulary, and indicate the development of language-specific phonetic timing in the course of acquisition.

In-cell NMR of intrinsically disordered proteins in prokaryotic cells.

PubMed

Ito, Yutaka; Mikawa, Tsutomu; Smith, Brian O

2012-01-01

In-cell NMR, i.e., the acquisition of heteronuclear multidimensional NMR of biomacromolecules inside living cells, is, to our knowledge, the only method for investigating the three-dimensional structure and dynamics of proteins at atomic detail in the intracellular environment. Since the inception of the method, intrinsically disordered proteins have been regarded as particular targets for in-cell NMR, due to their expected sensitivity to the molecular crowding in the intracellular environment. While both prokaryotic and eukaryotic cells can be used as host cells for in-cell NMR, prokaryotic in-cell NMR, particularly employing commonly used protein overexpression systems in Escherichia coli cells, is the most accessible approach. In this chapter we describe general procedures for obtaining in-cell NMR spectra in E. coli cells.

Sustaining Equipment and the Rapid Acquisition Process: The Forgotten Phase

DTIC Science & Technology

2012-02-24

Operation of the Defense Acquisition System,” December 8, 2008. 7 Rasch , Robert. A, Jr. Lessons Learned from Rapid Acquisition: Better, Faster, Cheaper...Life Cycle Management Responsibilities,” Defense AR Journal, 17.2 (April 2010): 183. 37 Robert A. Rasch , Lessons Learned from Rapid Acquisition: Better...Accountability Office (GAO) Report, Subject: Rapid Acquisition of Mine Resistant Protected Vehicles, July 15, 2008, 4. 39 Ibid. 40 Ibid. 41 Robert A. Rasch

Language acquisition is model-based rather than model-free.

PubMed

Wang, Felix Hao; Mintz, Toben H

2016-01-01

Christiansen & Chater (C&C) propose that learning language is learning to process language. However, we believe that the general-purpose prediction mechanism they propose is insufficient to account for many phenomena in language acquisition. We argue from theoretical considerations and empirical evidence that many acquisition tasks are model-based, and that different acquisition tasks require different, specialized models.

12 CFR 925.15 - Recent merger or acquisition applicants.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Recent merger or acquisition applicants. 925.15... ASSOCIATES MEMBERS OF THE BANKS Eligibility Requirements § 925.15 Recent merger or acquisition applicants. An... purposes of § 925.11(a)(1) of this part, an applicant that, as a result of a merger or acquisition...

Identifying Governance Best Practices in Systems-of-Systems Acquisition

DTIC Science & Technology

2014-02-08

Acquisition Governance ................................................ 28  Figure 3.  Harvest Hercules Airborne Weapons Kit System Components ...... 39 ...acquisition poses considerable challenges that the current Department of Defense (DoD) acquisition governance structure was not necessarily designed to...specify the warship it needed along with the design , construction, and outfitting of the ship. The Navy managed and performed production operations and

77 FR 59339 - Acquisition of Commercial Items

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-27

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 212 Acquisition of Commercial Items CFR Correction 212.504 [Corrected] In Title 48 of the Code of Federal Regulations, Chapter 2 (Parts 201--299), revised as of October 1, 2011, on page 73, in section 212.504, paragraph (a) is...

32 CFR 644.5 - Mineral acquisition practices.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 4 2012-07-01 2011-07-01 true Mineral acquisition practices. 644.5 Section 644... PROPERTY REAL ESTATE HANDBOOK Project Planning Civil Works § 644.5 Mineral acquisition practices. (a... accommodate projects authorized by the Congress is to permit the reservation of the minerals in the land...

32 CFR 644.5 - Mineral acquisition practices.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 4 2013-07-01 2013-07-01 false Mineral acquisition practices. 644.5 Section 644... PROPERTY REAL ESTATE HANDBOOK Project Planning Civil Works § 644.5 Mineral acquisition practices. (a... accommodate projects authorized by the Congress is to permit the reservation of the minerals in the land...

32 CFR 644.5 - Mineral Acquisition Practices.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 4 2010-07-01 2010-07-01 true Mineral Acquisition Practices. 644.5 Section 644... PROPERTY REAL ESTATE HANDBOOK Project Planning Civil Works § 644.5 Mineral Acquisition Practices. (a... accommodate projects authorized by the Congress is to permit the reservation of the minerals in the land...

32 CFR 644.5 - Mineral acquisition practices.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 4 2014-07-01 2013-07-01 true Mineral acquisition practices. 644.5 Section 644... PROPERTY REAL ESTATE HANDBOOK Project Planning Civil Works § 644.5 Mineral acquisition practices. (a... accommodate projects authorized by the Congress is to permit the reservation of the minerals in the land...

48 CFR 18.106 - Acquisitions from Federal Prison Industries, Inc. (FPI).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Acquisitions from Federal Prison Industries, Inc. (FPI). 18.106 Section 18.106 Federal Acquisition Regulations System FEDERAL... Flexibilities 18.106 Acquisitions from Federal Prison Industries, Inc. (FPI). Purchase from FPI is not mandatory...

48 CFR 18.106 - Acquisitions from Federal Prison Industries, Inc. (FPI).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Acquisitions from Federal Prison Industries, Inc. (FPI). 18.106 Section 18.106 Federal Acquisition Regulations System FEDERAL... Flexibilities 18.106 Acquisitions from Federal Prison Industries, Inc. (FPI). Purchase from FPI is not mandatory...

48 CFR 18.106 - Acquisitions from Federal Prison Industries, Inc. (FPI).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Acquisitions from Federal Prison Industries, Inc. (FPI). 18.106 Section 18.106 Federal Acquisition Regulations System FEDERAL... Flexibilities 18.106 Acquisitions from Federal Prison Industries, Inc. (FPI). Purchase from FPI is not mandatory...

48 CFR 18.106 - Acquisitions from Federal Prison Industries, Inc. (FPI).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Acquisitions from Federal Prison Industries, Inc. (FPI). 18.106 Section 18.106 Federal Acquisition Regulations System FEDERAL... Flexibilities 18.106 Acquisitions from Federal Prison Industries, Inc. (FPI). Purchase from FPI is not mandatory...

48 CFR 18.106 - Acquisitions from Federal Prison Industries, Inc. (FPI).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisitions from Federal Prison Industries, Inc. (FPI). 18.106 Section 18.106 Federal Acquisition Regulations System FEDERAL... Flexibilities 18.106 Acquisitions from Federal Prison Industries, Inc. (FPI). Purchase from FPI is not mandatory...

Securing Cyber Acquisitions

DTIC Science & Technology

2015-02-01

networking provides 24-hour access to data and information between friends and strangers alike. Technology also has played a significant role in the world’s...economic environment, many or- ganizations look at cyber budgets as areas to cut back. And many top-level managers and members of the acquisition

Data Acquisition and Mass Storage

NASA Astrophysics Data System (ADS)

Vande Vyvre, P.

2004-08-01

The experiments performed at supercolliders will constitute a new challenge in several disciplines of High Energy Physics and Information Technology. This will definitely be the case for data acquisition and mass storage. The microelectronics, communication, and computing industries are maintaining an exponential increase of the performance of their products. The market of commodity products remains the largest and the most competitive market of technology products. This constitutes a strong incentive to use these commodity products extensively as components to build the data acquisition and computing infrastructures of the future generation of experiments. The present generation of experiments in Europe and in the US already constitutes an important step in this direction. The experience acquired in the design and the construction of the present experiments has to be complemented by a large R&D effort executed with good awareness of industry developments. The future experiments will also be expected to follow major trends of our present world: deliver physics results faster and become more and more visible and accessible. The present evolution of the technologies and the burgeoning of GRID projects indicate that these trends will be made possible. This paper includes a brief overview of the technologies currently used for the different tasks of the experimental data chain: data acquisition, selection, storage, processing, and analysis. The major trends of the computing and networking technologies are then indicated with particular attention paid to their influence on the future experiments. Finally, the vision of future data acquisition and processing systems and their promise for future supercolliders is presented.

Uncoupling thermotolerance from the induction of heat shock proteins.

PubMed Central

Smith, B J; Yaffe, M P

1991-01-01

Exposure of cells to elevated temperatures causes a rapid increase in the synthesis of heat shock proteins (hsps) and induces thermotolerance, the increased ability of cells to survive exposure to lethal temperatures; however, the connection between hsp induction and the acquisition of thermotolerance is unclear. hsp induction in the yeast Saccharomyces cerevisiae is mediated by the activation of heat-shock transcription factor, and recently we have described a mutation, hsf1-m3, in heat-shock transcription factor that prevents the factor's activation. We now demonstrate that this mutation results in a general block in heat-shock induction but does not affect the acquisition of thermotolerance. Our results indicate that high-level induction of the major hsps is not required for cells to acquire thermotolerance. Images PMID:1763024

Acquisition with partial and continuous reinforcement in pigeon autoshaping.

PubMed

Gottlieb, Daniel A

2004-08-01

Contemporary time accumulation models make the unique prediction that acquisition of a conditioned response will be equally rapid with partial and continuous reinforcement, if the time between conditioned stimuli is held constant. To investigate this, acquisition of conditioned responding was examined in pigeon autoshaping under conditions of 100% and 25% reinforcement, holding intertrial interval constant. Contrary to what was predicted, evidence for slowed acquisition in partially reinforced animals was observed with several response measures. However, asymptotic performance was superior with 25% reinforcement. A switching of reinforcement contingencies after initial acquisition did not immediately affect responding. After further sessions, partial reinforcement augmented responding, whereas continuous reinforcement did not, irrespective of an animal's reinforcement history. Subsequent training with a novel stimulus maintained the response patterns. These acquisition results generally support associative, rather than time accumulation, accounts of conditioning.

Contributions of the Study of Japanese as a Second language to our General Understanding of Second Language Acquisition and the Definition of Second Language Acquisition Research.

ERIC Educational Resources Information Center

Wakabayashi, Shigenori

2003-01-01

Reviews three books on the acquisition of Japanese as a second language: "Second Language Acquisition Process in the Classroom" by A.S. Ohta;"The Acquisition of Grammar by Learners of Japanese" (English translation of title), by H. Noda, K. Sakoda, K. Shibuya, and N. Kobayashi; and "The Acquisition of Japanese as a Second Language," B. K. Kanno,…

48 CFR 2953.102 - Quotation for Simplified Acquisitions DL 1-2078.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Quotation for Simplified Acquisitions DL 1-2078. 2953.102 Section 2953.102 Federal Acquisition Regulations System DEPARTMENT OF LABOR CLAUSE AND FORMS FORMS General 2953.102 Quotation for Simplified Acquisitions DL 1-2078. The following...

Knowledge Acquisition Using Linguistic-Based Knowledge Analysis

Treesearch

Daniel L. Schmoldt

1998-01-01

Most knowledge-based system developmentefforts include acquiring knowledge from one or more sources. difficulties associated with this knowledge acquisition task are readily acknowledged by most researchers. While a variety of knowledge acquisition methods have been reported, little has been done to organize those different methods and to suggest how to apply them...

Proceedings of the Current Trends in Army Medical Department Psychology Held at San Antonio, Texas on 8-12 November 1976.

DTIC Science & Technology

1976-12-01

the faculty of several medical schools and universities. He is a 4 noted authority, lecturer, and author in the field of clinical hypnosis and has...a Fellow in the American Psychological Association and a Diplomate in Psychological Hypnosis . 0 .. ’..........- 1976 Current Trends in AMEDD...clinical neuropsychology, including the relationship of this field to medicine and various forensic concerns. Subsequent to this introduction by Dr

War-gaming application for future space systems acquisition part 2: acquisition and bidding war-gaming modeling and simulation approaches for FFP and FPIF

NASA Astrophysics Data System (ADS)

Nguyen, Tien M.; Guillen, Andy T.

2017-05-01

This paper describes cooperative and non-cooperative static Bayesian game models with complete and incomplete information for the development of optimum acquisition strategies associated with the Program and Technical Baseline (PTB) solutions obtained from Part 1 of this paper [1]. The optimum acquisition strategies discussed focus on achieving "Affordability" by incorporating contractors' bidding strategies into the government acquisition strategies for acquiring future space systems. The paper discusses System Engineering (SE) frameworks, analytical and simulation approaches and modeling for developing the optimum acquisition strategies from both the government and contractor perspectives for Firm Fixed Price (FFP) and Fixed Price Incentive Firm (FPIF) contract types.

DoD Weapon System Acquisition Reform Product Support Assessment

DTIC Science & Technology

2009-11-01

can be best effected by working through the Defense Acquisition University, the Logistics Functional IPT, the Acquisition Management Functional... WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Department of Defense,Undersecretary of Defense for Acquisition,Technology and...each year in product support. Reformed stewardship—driven by improving product support and achieving more cost- effective weapon system readiness

Auction and Game Theory Based Recommendations for DOD Acquisitions

DTIC Science & Technology

2015-03-24

SPONSORED REPORT SERIES Auction and Game Theory Based Recommendations for DOD Acquisitions 24 March 2015 Justin Blott, 2d Lt; Nicholas...TYPE 3. DATES COVERED 00-00-2015 to 00-00-2015 4. TITLE AND SUBTITLE Auction and Game Theory Based Recommendations for DOD Acquisitions 5a...Postgraduate School Abstract This paper synthesizes auction and game theory literature into specific military acquisition improvement

76 FR 13297 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-11

... 215 Government procurement. Ynette R. Shelkin, Editor, Defense Acquisition Regulations System... Director, Defense Procurement and Acquisition Policy. DATES: Effective Date: March 11, 2011. Applicability... adding a section at 215.300 with a reference to Director, Defense Procurement and Acquisition Policy...

78 FR 41331 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-10

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 225 Defense Federal Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Final rule. SUMMARY: DoD is making technical amendment to the Defense...

Expression of VGLUTs contributes to degeneration and acquisition of learning and memory.

PubMed

Cheng, Xiao-Rui; Yang, Yong; Zhou, Wen-Xia; Zhang, Yong-Xiang

2011-03-01

Vesicular glutamate transporters (VGLUTs), which include VGLUT1, VGLUT2 and VGLUT3, are responsible for the uploading of L-glutamate into synaptic vesicles. The expression pattern of VGLUTs determines the level of synaptic vesicle filling (i.e., glutamate quantal size) and directly influences glutamate receptors and glutamatergic synaptic transmission; thus, VGLUTs may play a key role in learning and memory in the central nervous system. To determine whether VGLUTs contribute to the degeneration or acquisition of learning and memory, we used an animal model for the age-related impairment of learning and memory, senescence-accelerated mouse/prone 8 (SAMP8). KM mice were divided into groups based on their learning and memory performance in a shuttle-box test. The expression of VGLUTs and synaptophysin (Syp) mRNA and protein in the cerebral cortex and hippocampus were investigated with real-time fluorescence quantitative PCR and western blot, respectively. Our results demonstrate that, in the cerebral cortex, protein expression of VGLUT1, VGLUT2, VGLUT3 and Syp was decreased in SAMP8 with age and increased in KM mice, which displayed an enhanced capacity for learning and memory. The protein expression of VGLUT2 and Syp was decreased in the hippocampus of SAMP8 with aging. The expression level of VGLUT1 and VGLUT2 proteins were highest in KM mouse group with a 76-100% avoidance score in the shuttle-box test. These data demonstrate that protein expression of VGLUT1, VGLUT2 and Syp decreases age-dependently in SAMP8 and increases in a learning- and memory-dependent manner in KM mice. Correlation analysis indicated the protein expression of VGLUT1, VGLUT2 and Syp has a positive correlation with the capacity of learning and memory. Copyright © 2011 Elsevier Inc. All rights reserved.

Sequencing Larger Intact Proteins (30-70 kDa) with Activated Ion Electron Transfer Dissociation

NASA Astrophysics Data System (ADS)

Riley, Nicholas M.; Westphall, Michael S.; Coon, Joshua J.

2018-01-01

The analysis of intact proteins via mass spectrometry can offer several benefits to proteome characterization, although the majority of top-down experiments focus on proteoforms in a relatively low mass range (<30 kDa). Recent studies have focused on improving the analysis of larger intact proteins (up to 75 kDa), but they have also highlighted several challenges to be addressed. One major hurdle is the efficient dissociation of larger protein ions, which often to do not yield extensive fragmentation via conventional tandem MS methods. Here we describe the first application of activated ion electron transfer dissociation (AI-ETD) to proteins in the 30-70 kDa range. AI-ETD leverages infrared photo-activation concurrent to ETD reactions to improve sequence-informative product ion generation. This method generates more product ions and greater sequence coverage than conventional ETD, higher-energy collisional dissociation (HCD), and ETD combined with supplemental HCD activation (EThcD). Importantly, AI-ETD provides the most thorough protein characterization for every precursor ion charge state investigated in this study, making it suitable as a universal fragmentation method in top-down experiments. Additionally, we highlight several acquisition strategies that can benefit characterization of larger proteins with AI-ETD, including combination of spectra from multiple ETD reaction times for a given precursor ion, multiple spectral acquisitions of the same precursor ion, and combination of spectra from two different dissociation methods (e.g., AI-ETD and HCD). In all, AI-ETD shows great promise as a method for dissociating larger intact protein ions as top-down proteomics continues to advance into larger mass ranges. [Figure not available: see fulltext.

Practical aspects of NMR signal assignment in larger and challenging proteins

PubMed Central

Frueh, Dominique P.

2014-01-01

NMR has matured into a technique routinely employed for studying proteins in near physiological conditions. However, applications to larger proteins are impeded by the complexity of the various correlation maps necessary to assign NMR signals. This article reviews the data analysis techniques traditionally employed for resonance assignment and describes alternative protocols necessary for overcoming challenges in large protein spectra. In particular, simultaneous analysis of multiple spectra may help overcome ambiguities or may reveal correlations in an indirect manner. Similarly, visualization of orthogonal planes in a multidimensional spectrum can provide alternative assignment procedures. We describe examples of such strategies for assignment of backbone, methyl, and nOe resonances. We describe experimental aspects of data acquisition for the related experiments and provide guidelines for preliminary studies. Focus is placed on large folded monomeric proteins and examples are provided for 37, 48, 53, and 81 kDa proteins. PMID:24534088

The image acquisition system design of floor grinder

NASA Astrophysics Data System (ADS)

Wang, Yang-jiang; Liu, Wei; Liu, Hui-qin

2018-01-01

Based on linear CCD, high resolution image real-time acquisition system serves as designing a set of image acquisition system for floor grinder through the calculation of optical imaging system. The entire image acquisition system can collect images of ground before and after the work of the floor grinder, and the data is transmitted through the Bluetooth system to the computer and compared to realize real-time monitoring of its working condition. The system provides technical support for the design of unmanned ground grinders.

The pathway by which the yeast protein kinase Snf1p controls acquisition of sodium tolerance is different from that mediating glucose regulation.

PubMed

Ye, Tian; Elbing, Karin; Hohmann, Stefan

2008-09-01

It recently became apparent that the highly conserved Snf1p protein kinase plays roles in controlling different cellular processes in the yeast Saccharomyces cerevisiae, in addition to its well-known function in glucose repression/derepression. We have previously reported that Snf1p together with Gis4p controls ion homeostasis by regulating expression of ENA1, which encodes the Ena1p Na(+) extrusion system. In this study we found that Snf1p is rapidly phosphorylated when cells are exposed to NaCl and this phosphorylation is required for the role of Snf1p in Na(+) tolerance. In contrast to activation by low glucose levels, the salt-induced phosphorylation of Snf1p promoted neither phosphorylation nor nuclear export of the Mig1p repressor. The mechanism that prevents Mig1p phosphorylation by active Snf1p under salt stress does not involve either hexokinase PII or the Gis4p regulator. Instead, Snf1p may mediate upregulation of ENA1 expression via the repressor Nrg1p. Activation of Snf1p in response to glucose depletion requires any of the three upstream protein kinases Sak1p, Tos3p and Elm1p, with Sak1p playing the most prominent role. The same upstream kinases were required for salt-induced Snf1p phosphorylation, and also under these conditions Sak1p played the most prominent role. Unexpectedly, however, it appears that Elm1p plays a dual role in acquisition of salt tolerance by activating Snf1p and in a presently unknown parallel pathway. Together, these results indicate that under salt stress Snf1p takes part in a different pathway from that during glucose depletion and this role is performed together as well as in parallel with its upstream kinase Elm1p. Snf1p appears to be part of a wider functional network than previously anticipated and the full complexity of this network remains to be elucidated.

78 FR 13543 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-28

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Parts 201, 204, 215, 225, 227, 242, 245, and 252 Defense Federal Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Final rule. SUMMARY: DoD is...

78 FR 38533 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-68; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-26

... Administration National Aeronautics and Space Administration 48 CFR Chapter 1 Federal Acquisition Regulations... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION 48 CFR Chapter 1 [Docket FAR 2013-0076, Sequence 4] Federal...), General Services Administration (GSA), and National Aeronautics and Space Administration (NASA). ACTION...

School Library Acquisitions: A Model for Calculating Costs

ERIC Educational Resources Information Center

Lauterman, Alfred; Lazarescu, Sandu

1977-01-01

Romanian research findings offer a theoretical model with which financing of the annual acquisition of books per pupil at a given educational level can be objectively ascertained. Methods of financing and acquisitions policy decisions are discussed. (Author/JAB)

48 CFR 1532.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... procedures financing. 1532.003 Section 1532.003 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING 1532.003 Simplified acquisition procedures financing. (a) Scope. This subpart provides for authorization of advance and interim payments on...

Computer-Aided Process and Tools for Mobile Software Acquisition

DTIC Science & Technology

2013-04-01

Software Acquisition Christopher Bonine , Man-Tak Shing, and Thomas W. Otani Naval Postgraduate School Published April 1, 2013 Approved for public...ManTech International Corporation Computer-Aided Process and Tools for Mobile Software Acquisition Christopher Bonine , Man-Tak Shing, and Thomas W. Otani...Mobile Software Acquisition Christopher Bonine — Bonine is a lieutenant in the United States Navy. He is currently assigned to the Navy Cyber Defense

Graphical user interface for image acquisition and processing

DOEpatents

Goldberg, Kenneth A.

2002-01-01

An event-driven GUI-based image acquisition interface for the IDL programming environment designed for CCD camera control and image acquisition directly into the IDL environment where image manipulation and data analysis can be performed, and a toolbox of real-time analysis applications. Running the image acquisition hardware directly from IDL removes the necessity of first saving images in one program and then importing the data into IDL for analysis in a second step. Bringing the data directly into IDL creates an opportunity for the implementation of IDL image processing and display functions in real-time. program allows control over the available charge coupled device (CCD) detector parameters, data acquisition, file saving and loading, and image manipulation and processing, all from within IDL. The program is built using IDL's widget libraries to control the on-screen display and user interface.

12 CFR 225.132 - Acquisition of assets.

Code of Federal Regulations, 2010 CFR

2010-01-01

... it constitutes the acquisition, in whole or in part, of a going concern. 2 1 Section 225.4(c)(3) of... Regulation Y, the acquisition of a going concern requires prior Board approval. (c) The following examples... going concern. [39 FR 35128, Sept. 30, 1974, as amended at Reg. Y, 57 FR 28779, June 29, 1992] ...

10 CFR 626.5 - Acquisition procedures-general.

Code of Federal Regulations, 2014 CFR

2014-01-01

... acquire petroleum for the SPR. The notice of acquisition is usually in the form of a solicitation. DOE... the Web sites of the DOE Office of Fossil Energy http://www.fe.doe.gov/programs/reserves and the OPR... acquisition solicitations, and shall make them available on the Web sites of the DOE Office of Fossil Energy...

10 CFR 626.5 - Acquisition procedures-general.

Code of Federal Regulations, 2010 CFR

2010-01-01

... acquire petroleum for the SPR. The notice of acquisition is usually in the form of a solicitation. DOE... the Web sites of the DOE Office of Fossil Energy http://www.fe.doe.gov/programs/reserves and the OPR... acquisition solicitations, and shall make them available on the Web sites of the DOE Office of Fossil Energy...

10 CFR 626.5 - Acquisition procedures-general.

Code of Federal Regulations, 2012 CFR

2012-01-01

... acquire petroleum for the SPR. The notice of acquisition is usually in the form of a solicitation. DOE... the Web sites of the DOE Office of Fossil Energy http://www.fe.doe.gov/programs/reserves and the OPR... acquisition solicitations, and shall make them available on the Web sites of the DOE Office of Fossil Energy...

10 CFR 626.5 - Acquisition procedures-general.

Code of Federal Regulations, 2013 CFR

2013-01-01

... acquire petroleum for the SPR. The notice of acquisition is usually in the form of a solicitation. DOE... the Web sites of the DOE Office of Fossil Energy http://www.fe.doe.gov/programs/reserves and the OPR... acquisition solicitations, and shall make them available on the Web sites of the DOE Office of Fossil Energy...

10 CFR 626.5 - Acquisition procedures-general.

Code of Federal Regulations, 2011 CFR

2011-01-01

... acquire petroleum for the SPR. The notice of acquisition is usually in the form of a solicitation. DOE... the Web sites of the DOE Office of Fossil Energy http://www.fe.doe.gov/programs/reserves and the OPR... acquisition solicitations, and shall make them available on the Web sites of the DOE Office of Fossil Energy...

Defense Acquisitions: Assessments of Selected Weapon Programs

DTIC Science & Technology

2015-03-01

offices on technology, design , and manufacturing knowledge; the use of knowledge- based acquisition practices; and the implementation of acquisition...and production maturity using two data-collection instruments, including a questionnaire on issues such as systems engineering reviews, design ...Demonstrating technology maturity is a prerequisite for moving forward into system development, during which the focus should be on design and

48 CFR 252.225-7011 - Restriction on acquisition of supercomputers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... of supercomputers. 252.225-7011 Section 252.225-7011 Federal Acquisition Regulations System DEFENSE... CLAUSES Text of Provisions And Clauses 252.225-7011 Restriction on acquisition of supercomputers. As prescribed in 225.7012-3, use the following clause: Restriction on Acquisition of Supercomputers (JUN 2005...

48 CFR 252.225-7011 - Restriction on acquisition of supercomputers.

Code of Federal Regulations, 2014 CFR

2014-10-01

... of supercomputers. 252.225-7011 Section 252.225-7011 Federal Acquisition Regulations System DEFENSE... CLAUSES Text of Provisions And Clauses 252.225-7011 Restriction on acquisition of supercomputers. As prescribed in 225.7012-3, use the following clause: Restriction on Acquisition of Supercomputers (JUN 2005...

48 CFR 252.225-7011 - Restriction on acquisition of supercomputers.

Code of Federal Regulations, 2012 CFR

2012-10-01

... of supercomputers. 252.225-7011 Section 252.225-7011 Federal Acquisition Regulations System DEFENSE... CLAUSES Text of Provisions And Clauses 252.225-7011 Restriction on acquisition of supercomputers. As prescribed in 225.7012-3, use the following clause: Restriction on Acquisition of Supercomputers (JUN 2005...

48 CFR 252.225-7011 - Restriction on acquisition of supercomputers.

Code of Federal Regulations, 2013 CFR

2013-10-01

... of supercomputers. 252.225-7011 Section 252.225-7011 Federal Acquisition Regulations System DEFENSE... CLAUSES Text of Provisions And Clauses 252.225-7011 Restriction on acquisition of supercomputers. As prescribed in 225.7012-3, use the following clause: Restriction on Acquisition of Supercomputers (JUN 2005...

48 CFR 252.225-7011 - Restriction on acquisition of supercomputers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... of supercomputers. 252.225-7011 Section 252.225-7011 Federal Acquisition Regulations System DEFENSE... CLAUSES Text of Provisions And Clauses 252.225-7011 Restriction on acquisition of supercomputers. As prescribed in 225.7012-3, use the following clause: Restriction on Acquisition of Supercomputers (JUN 2005...

Synthetic aperture radar operator tactical target acquisition research

NASA Technical Reports Server (NTRS)

Hershberger, M. L.; Craig, D. W.

1978-01-01

A radar target acquisition research study was conducted to access the effects of two levels of 13 radar sensor, display, and mission parameters on operator tactical target acquisition. A saturated fractional-factorial screening design was employed to examine these parameters. Data analysis computed ETA squared values for main and second-order effects for the variables tested. Ranking of the research parameters in terms of importance to system design revealed four variables (radar coverage, radar resolution/multiple looks, display resolution, and display size) accounted for 50 percent of the target acquisition probability variance.

Resistance Mechanisms and the Future of Bacterial Enoyl-Acyl Carrier Protein Reductase (FabI) Antibiotics

PubMed Central

Yao, Jiangwei; Rock, Charles O.

2016-01-01

Missense mutations leading to clinical antibiotic resistance are a liability of single-target inhibitors. The enoyl-acyl carrier protein reductase (FabI) inhibitors have one intracellular protein target and drug resistance is increased by the acquisition of single-base-pair mutations that alter drug binding. The spectrum of resistance mechanisms to FabI inhibitors suggests criteria that should be considered during the development of single-target antibiotics that would minimize the impact of missense mutations on their clinical usefulness. These criteria include high-affinity, fast on/off kinetics, few drug contacts with residue side chains, and no toxicity. These stringent criteria are achievable by structure-guided design, but this approach will only yield pathogen-specific drugs. Single-step acquisition of resistance may limit the clinical application of broad-spectrum, single-target antibiotics, but appropriately designed pathogen-specific antibiotics have the potential to overcome this liability. PMID:26931811

78 FR 32214 - Land Acquisitions: Appeals of Land Acquisition Decisions

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-29

...; 134D0102DR-DS5A300000-DR.5A311.IA000113; Docket ID: BIA-2013-0005] RIN 1076-AF15 Land Acquisitions: Appeals... trust under this part, including broadening notice of any right to file an administrative appeal. DATES... the United States acquired title. See, e.g., Neighbors for Rational Dev., Inc. v. Norton, 379 F.3d 956...

Iconicity and Sign Lexical Acquisition: A Review

PubMed Central

Ortega, Gerardo

2017-01-01

The study of iconicity, defined as the direct relationship between a linguistic form and its referent, has gained momentum in recent years across a wide range of disciplines. In the spoken modality, there is abundant evidence showing that iconicity is a key factor that facilitates language acquisition. However, when we look at sign languages, which excel in the prevalence of iconic structures, there is a more mixed picture, with some studies showing a positive effect and others showing a null or negative effect. In an attempt to reconcile the existing evidence the present review presents a critical overview of the literature on the acquisition of a sign language as first (L1) and second (L2) language and points at some factor that may be the source of disagreement. Regarding sign L1 acquisition, the contradicting findings may relate to iconicity being defined in a very broad sense when a more fine-grained operationalisation might reveal an effect in sign learning. Regarding sign L2 acquisition, evidence shows that there is a clear dissociation in the effect of iconicity in that it facilitates conceptual-semantic aspects of sign learning but hinders the acquisition of the exact phonological form of signs. It will be argued that when we consider the gradient nature of iconicity and that signs consist of a phonological form attached to a meaning we can discern how iconicity impacts sign learning in positive and negative ways. PMID:28824480

48 CFR 252.225-7025 - Restriction on acquisition of forgings.

Code of Federal Regulations, 2010 CFR

2010-10-01

... of forgings. 252.225-7025 Section 252.225-7025 Federal Acquisition Regulations System DEFENSE... CLAUSES Text of Provisions And Clauses 252.225-7025 Restriction on acquisition of forgings. As prescribed in 225.7102-4, use the following clause: Restriction on Acquisition of Forgings (DEC 2009) (a...

48 CFR 1332.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... procedures financing. 1332.003 Section 1332.003 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING 1332.003 Simplified acquisition procedures financing. Contract financing may be provided for purchases made under the authority of FAR Part 13. Contract...

48 CFR 432.003 - Simplified acquisition procedures financing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... procedures financing. 432.003 Section 432.003 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING 432.003 Simplified acquisition procedures financing. (a) The chief of the contracting office may approve contract financing on a contract to be...

Magnetic Field Response Measurement Acquisition System

NASA Technical Reports Server (NTRS)

Woodard, Stanley E.; Taylor,Bryant D.; Shams, Qamar A.; Fox, Robert L.

2007-01-01

This paper presents a measurement acquisition method that alleviates many shortcomings of traditional measurement systems. The shortcomings are a finite number of measurement channels, weight penalty associated with measurements, electrical arcing, wire degradations due to wear or chemical decay and the logistics needed to add new sensors. Wire degradation has resulted in aircraft fatalities and critical space launches being delayed. The key to this method is the use of sensors designed as passive inductor-capacitor circuits that produce magnetic field responses. The response attributes correspond to states of physical properties for which the sensors measure. Power is wirelessly provided to the sensing element by using Faraday induction. A radio frequency antenna produces a time-varying magnetic field used to power the sensor and receive the magnetic field response of the sensor. An interrogation system for discerning changes in the sensor response frequency, resistance and amplitude has been developed and is presented herein. Multiple sensors can be interrogated using this method. The method eliminates the need for a data acquisition channel dedicated to each sensor. The method does not require the sensors to be near the acquisition hardware. Methods of developing magnetic field response sensors and the influence of key parameters on measurement acquisition are discussed. Examples of magnetic field response sensors and the respective measurement characterizations are presented. Implementation of this method on an aerospace system is discussed.

Fixed-target protein serial microcrystallography with an x-ray free electron laser

PubMed Central

Hunter, Mark S.; Segelke, Brent; Messerschmidt, Marc; Williams, Garth J.; Zatsepin, Nadia A.; Barty, Anton; Benner, W. Henry; Carlson, David B.; Coleman, Matthew; Graf, Alexander; Hau-Riege, Stefan P.; Pardini, Tommaso; Seibert, M. Marvin; Evans, James; Boutet, Sébastien; Frank, Matthias

2014-01-01

We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution (~2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using x-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared to flowing jet methods. We achieved a peak data acquisition rate of 10 Hz with a hit rate of ~38%, indicating that a complete data set could be acquired in about one 12-hour LCLS shift using the setup described here, or in even less time using hardware optimized for fixed target SFX. This demonstration opens the door to ultra low sample consumption SFX using the technique of diffraction-before-destruction on proteins that exist in only small quantities and/or do not produce the copious quantities of microcrystals required for flowing jet methods. PMID:25113598

AdaNET Dynamic Software Inventory (DSI) prototype component acquisition plan

NASA Technical Reports Server (NTRS)

Hanley, Lionel

1989-01-01

A component acquisition plan contains the information needed to evaluate, select, and acquire software and hardware components necessary for successful completion of the AdaNET Dynamic Software Inventory (DSI) Management System Prototype. This plan will evolve and be applicable to all phases of the DSI prototype development. Resources, budgets, schedules, and organizations related to component acquisition activities are provided. A purpose and description of a software or hardware component which is to be acquired are presented. Since this is a plan for acquisition of all components, this section is not applicable. The procurement activities and events conducted by the acquirer are described and who is responsible is identified, where the activity will be performed, and when the activities will occur for each planned procurement. Acquisition requirements describe the specific requirements and standards to be followed during component acquisition. The activities which will take place during component acquisition are described. A list of abbreviations and acronyms, and a glossary are contained.

Computational Modeling for Language Acquisition: A Tutorial With Syntactic Islands.

PubMed

Pearl, Lisa S; Sprouse, Jon

2015-06-01

Given the growing prominence of computational modeling in the acquisition research community, we present a tutorial on how to use computational modeling to investigate learning strategies that underlie the acquisition process. This is useful for understanding both typical and atypical linguistic development. We provide a general overview of why modeling can be a particularly informative tool and some general considerations when creating a computational acquisition model. We then review a concrete example of a computational acquisition model for complex structural knowledge referred to as syntactic islands. This includes an overview of syntactic islands knowledge, a precise definition of the acquisition task being modeled, the modeling results, and how to meaningfully interpret those results in a way that is relevant for questions about knowledge representation and the learning process. Computational modeling is a powerful tool that can be used to understand linguistic development. The general approach presented here can be used to investigate any acquisition task and any learning strategy, provided both are precisely defined.

75 FR 1567 - Defense Federal Acquisition Regulation Supplement; Foreign Participation in Acquisitions in...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-12

... acquisition from the nine South Caucasus/Central and South Asian (SC/CASA) states; and Determination of..., ammunition, or war materials) from the SC/CASA states to support operations in Afghanistan. The end of the...

No easy answers: issues in accounting for hospital acquisitions.

PubMed

Bernstein, K R; Alexander, D E

1986-07-01

A real and often overlooked concern created by the Deficit Reduction Act of 1984 is the effect these amendments have on the cash flow generated by an acquisition. Financial managers, therefore, should be aware of the accounting issues created by the act--depreciation and its effect on appraised values, reevaluation to the present value of assumed indebtness, and capitalization of acquisition costs--to accurately evaluate the feasibility of an acquisition.

In Pursuit of Agile Acquisition: Are We There Yet?

DTIC Science & Technology

2013-03-01

digital mapping capabilities like Google , 71Microsoft,72 and Wikimapia,73 are readily obtainable in the commercial marketplace. This knowledge...Fox. Defense Acquisition Reform, 14. 69 Ibid., 8. 70 XBRADTC, “Army Acquisition Woes,” Bring the Heat Bring the Stupid , entry posted May 1, 2011...https://xbradtc.wordpress.com/2011/05/01/Army-acquisition-woes/ (accessed on December 5, 2012). 71 Google Maps, http://maps.google.com/maps (accessed

Surviving mergers & acquisitions.

PubMed

Dixon, Diane L

2002-01-01

Mergers and acquisitions are never easy to implement. The health care landscape is a minefield of failed mergers and uneasy alliances generating great turmoil and pain. But some mergers have been successful, creating health systems that benefit the communities they serve. Five prominent leaders offer their advice on minimizing the difficulties of M&As.

Thermal (Silicon Diode) Data Acquisition Systems

NASA Technical Reports Server (NTRS)

Wright, Ernest; Kegley, Jeff

2008-01-01

Marshall Space Flight Center s X-ray Cryogenic Facility (XRCF) has been performing cryogenic testing to 20 Kelvin since 1999. Two configurations for acquiring data from silicon diode temperature sensors have been implemented at the facility. The facility's environment is recorded via a data acquisition system capable of reading up to 60 silicon diodes. Test article temperature is recorded by a second data acquisition system capable of reading 150+ silicon diodes. The specifications and architecture of both systems will be presented.

Thermal (Silicon Diode) Data Acquisition System

NASA Technical Reports Server (NTRS)

Kegley, Jeffrey

2008-01-01

Marshall Space Flight Center's X-ray Calibration Facility (XRCF) has been performing cryogenic testing to 20 Kelvin since 1999. Two configurations for acquiring data from silicon diode temperature sensors have been implemented at the facility. The facility's environment is recorded via a data acquisition system capable of reading up to 60 silicon diodes. Test article temperature is recorded by a second data acquisition system capable of reading 150+ silicon diodes. The specifications and architecture of both systems will be presented.

Cost modeling to justify technology acquisitions.

PubMed

Vanden Brink, J; Gray, S

1997-06-01

In an era of diminishing resources, healthcare providers must justify new technology acquisitions. Cost modeling is one method of evaluating the financial impact a technology acquisition will have on a healthcare facility or integrated delivery system. This methodology requires careful data collection and a thorough analysis of both current costs and future cost savings resulting from the new technology. By using a cost modeling methodology, providers will be able to achieve competitive and economic advantages by analyzing both cost and value.

Designing a Graphical Decision Support Tool to Improve System Acquisition Decisions

DTIC Science & Technology

2009-06-01

relationships within the data [9]. Displaying acquisition data in a graphical manner was chosen because graphical formats, in general, have been...acquisition plan which includes information pertaining to the acquisition objectives, the required capability of the system, design trade-off, budgeting...which introduce artificial neural networks to approximate the real world experience of an acquisition manager [8]. However, these strategies lack a

Acquisition Streamlining

DTIC Science & Technology

1986-12-01

perform its mission when called upon to do so, as evidenced by the aborted Iranian hostage rescue. The scenario described has been a low point in the...National Maintenance Point , U.S. Army Security Agency Materiel Support Command, and Charles A . McCarthy, stated in 1976, that the specification for...integral Part of the acquisition process (Ref. 2]. In a memorandum dated December 4, 1984, Mr. Taft identi- fied as the point of contact for the office of

Federal Library Programs for Acquisition of Foreign Materials.

ERIC Educational Resources Information Center

Cylke, Frank Kurt

Sixteen libraries representing those agencies holding membership on the Federal Library Committee were surveyed to determine library foreign language or imprint holdings, acquisitions techniques, procedures and/or problems. Specific questions, relating to holdings, staff, budget and the acquisition, processing, reference and translation services…

77 FR 12937 - Federal Acquisition Regulation; Government Property

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-02

... 2010-0009, Sequence 1] RIN 9000-AL95 Federal Acquisition Regulation; Government Property AGENCIES... Federal Acquisition Regulation (FAR) to clarify reporting, reutilization, and disposal of Government... used consistently throughout the FAR, e.g., ``loss of Government property'' is defined at FAR 45.101...

48 CFR 301.270 - Executive Committee for Acquisition.

Code of Federal Regulations, 2011 CFR

2011-10-01

...). (10) Program Support Center (PSC). (11) Substance Abuse and Mental Health Services Administration... Acquisition. 301.270 Section 301.270 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL... Medicare and Medicaid Services (CMS). (6) Food and Drug Administration (FDA). (7) Health Resources and...

48 CFR 301.270 - Executive Committee for Acquisition.

Code of Federal Regulations, 2010 CFR

2010-10-01

...). (10) Program Support Center (PSC). (11) Substance Abuse and Mental Health Services Administration... Acquisition. 301.270 Section 301.270 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL... Medicare and Medicaid Services (CMS). (6) Food and Drug Administration (FDA). (7) Health Resources and...

75 FR 59102 - Defense Federal Acquisition Regulation Supplement; Part 204, Administrative Matters

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

... indexing methodology across DoD. This technical amendment adds language to the Defense Federal Acquisition... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 204 Defense Federal Acquisition Regulation Supplement; Part 204, Administrative Matters AGENCY: Defense Acquisition Regulations...

48 CFR 217.7504 - Acquisition of parts when data is not available.

Code of Federal Regulations, 2012 CFR

2012-10-01

... data is not available. 217.7504 Section 217.7504 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SPECIAL CONTRACTING METHODS Acquisition of Replenishment Parts 217.7504 Acquisition of parts when data is not...

48 CFR 217.7504 - Acquisition of parts when data is not available.

Code of Federal Regulations, 2011 CFR

2011-10-01

... data is not available. 217.7504 Section 217.7504 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SPECIAL CONTRACTING METHODS Acquisition of Replenishment Parts 217.7504 Acquisition of parts when data is not...

48 CFR 217.7504 - Acquisition of parts when data is not available.

Code of Federal Regulations, 2010 CFR

2010-10-01

... data is not available. 217.7504 Section 217.7504 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SPECIAL CONTRACTING METHODS Acquisition of Replenishment Parts 217.7504 Acquisition of parts when data is not...

48 CFR 217.7504 - Acquisition of parts when data is not available.

Code of Federal Regulations, 2013 CFR

2013-10-01

... data is not available. 217.7504 Section 217.7504 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SPECIAL CONTRACTING METHODS Acquisition of Replenishment Parts 217.7504 Acquisition of parts when data is not...

48 CFR 217.7504 - Acquisition of parts when data is not available.

Code of Federal Regulations, 2014 CFR

2014-10-01

... data is not available. 217.7504 Section 217.7504 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SPECIAL CONTRACTING METHODS Acquisition of Replenishment Parts 217.7504 Acquisition of parts when data is not...

Oxytocin differentially modulates pavlovian cue and context fear acquisition.

PubMed

Cavalli, Juliana; Ruttorf, Michaela; Pahi, Mario Rosero; Zidda, Francesca; Flor, Herta; Nees, Frauke

2017-06-01

Fear acquisition and extinction have been demonstrated as core mechanisms for the development and maintenance of mental disorders, with different contributions of processing cues vs contexts. The hypothalamic peptide oxytocin (OXT) may have a prominent role in this context, as it has been shown to affect fear learning. However, investigations have focused on cue conditioning, and fear extinction. Its differential role for cue and context fear acquisition is still not known. In a randomized, double-blind, placebo (PLC)-controlled design, we administered an intranasal dose of OXT or PLC before the acquisition of cue and context fear conditioning in healthy individuals (n = 52), and assessed brain responses, skin conductance responses and self-reports (valence/arousal/contingency). OXT compared with PLC significantly induced decreased responses in the nucleus accumbens during early cue and context acquisition, and decreased responses of the anterior cingulate cortex and insula during early as well as increased hippocampal response during late context, but not cue acquisition. The OXT group additionally showed significantly higher arousal in late cue and context acquisition. OXT modulates various aspects of cue and context conditioning, which is relevant from a mechanism-based perspective and might have implications for the treatment of fear and anxiety. © The Author (2017). Published by Oxford University Press.

Hunger promotes acquisition of nonfood objects.

PubMed

Xu, Alison Jing; Schwarz, Norbert; Wyer, Robert S

2015-03-03

Hunger motivates people to consume food, for which finding and acquiring food is a prerequisite. We test whether the acquisition component spills over to nonfood objects: Are hungry people more likely to acquire objects that cannot satisfy their hunger? Five laboratory and field studies show that hunger increases the accessibility of acquisition-related concepts and the intention to acquire not only food but also nonfood objects. Moreover, people act on this intention and acquire more nonfood objects (e.g., binder clips) when they are hungry, both when these items are freely available and when they must be paid for. However, hunger does not influence how much they like nonfood objects. We conclude that a basic biologically based motivation can affect substantively unrelated behaviors that cannot satisfy the motivation. This presumably occurs because hunger renders acquisition-related concepts and behaviors more accessible, which influences decisions in situations to which they can be applied.

Magnetic Field Response Measurement Acquisition System

NASA Technical Reports Server (NTRS)

Woodard, Stanley E.; Taylor, Bryant D.; Shams, Qamar A.; Fox, Robert L.

2005-01-01

A measurement acquisition method that alleviates many shortcomings of traditional measurement systems is presented in this paper. The shortcomings are a finite number of measurement channels, weight penalty associated with measurements, electrical arcing, wire degradations due to wear or chemical decay and the logistics needed to add new sensors. The key to this method is the use of sensors designed as passive inductor-capacitor circuits that produce magnetic field responses. The response attributes correspond to states of physical properties for which the sensors measure. A radio frequency antenna produces a time-varying magnetic field used to power the sensor and receive the magnetic field response of the sensor. An interrogation system for discerning changes in the sensor response is presented herein. Multiple sensors can be interrogated using this method. The method eliminates the need for a data acquisition channel dedicated to each sensor. Methods of developing magnetic field response sensors and the influence of key parameters on measurement acquisition are discussed.

Hunger promotes acquisition of nonfood objects

PubMed Central

Xu, Alison Jing; Schwarz, Norbert; Wyer, Robert S.

2015-01-01

Hunger motivates people to consume food, for which finding and acquiring food is a prerequisite. We test whether the acquisition component spills over to nonfood objects: Are hungry people more likely to acquire objects that cannot satisfy their hunger? Five laboratory and field studies show that hunger increases the accessibility of acquisition-related concepts and the intention to acquire not only food but also nonfood objects. Moreover, people act on this intention and acquire more nonfood objects (e.g., binder clips) when they are hungry, both when these items are freely available and when they must be paid for. However, hunger does not influence how much they like nonfood objects. We conclude that a basic biologically based motivation can affect substantively unrelated behaviors that cannot satisfy the motivation. This presumably occurs because hunger renders acquisition-related concepts and behaviors more accessible, which influences decisions in situations to which they can be applied. PMID:25730858

77 FR 13951 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-57; Introduction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-07

... Trade Organization Government Procurement Agreement (WTO GPA). This Free Trade Agreement now covers acquisition of supplies and services between $100,000 and the current WTO GPA threshold of $202,000. This... effective March 15, 2012. Dated: March 1, 2012. Richard Ginman, Director, Defense Procurement and...