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Sample records for acquisition protein hasa

  1. Replacing Arginine 33 for Alanine in the Hemophore HasA from Pseudomonas aeruginosa Causes Closure of the H32 Loop in the Apo-Protein.

    PubMed

    Kumar, Ritesh; Qi, Yifei; Matsumura, Hirotoshi; Lovell, Scott; Yao, Huili; Battaile, Kevin P; Im, Wonpil; Moënne-Loccoz, Pierre; Rivera, Mario

    2016-05-10

    Previous characterization of hemophores from Serratia marcescens (HasAs), Pseudomonas aeruginosa (HasAp), and Yersinia pestis (HasAyp) showed that hemin binds between two loops, where it is axially coordinated by H32 and Y75. The Y75 loop is structurally conserved in all three hemophores and harbors conserved ligand Y75. The other loop contains H32 in HasAs and HasAp, but a noncoordinating Q32 in HasAyp. The H32 loop in apo-HasAs and apo-HasAp is in an open conformation, which places H32 about 30 Å from the hemin-binding site. Hence, hemin binding onto the Y75 loop of HasAs or HasAp triggers a large relocation of the H32 loop from an open- to a closed-loop conformation and enables coordination of the hemin-iron by H32. In comparison, the Q32 loop in apo-HasAyp is in the closed conformation, and hemin binding occurs with minimal reorganization and without coordinative interactions with the Q32 loop. Studies in crystallo and in solution have established that the open H32 loop in apo-HasAp and apo-HasAs is well structured and minimally affected by conformational dynamics. In this study we address the intriguing issue of the stability of the H32 loop in apo-HasAp and how hemin binding triggers its relocation. We address this question with a combination of NMR spectroscopy, X-ray crystallography, and molecular dynamics simulations and find that R33 is critical to the stability of the open H32 loop. Replacing R33 with A causes the H32 loop in R33A apo-HasAp to adopt a conformation similar to that of holo-HasAp. Finally, stopped-flow absorption and resonance Raman analyses of hemin binding to apo-R33A HasAp indicate that the closed H32 loop slows down the insertion of the heme inside the binding pocket, presumably as it obstructs access to the hydrophobic platform on the Y75 loop, but accelerates the completion of the heme iron coordination. PMID:27074415

  2. Interactions of HasA, a bacterial haemophore, with haemoglobin and with its outer membrane receptor HasR.

    PubMed

    Létoffé, S; Nato, F; Goldberg, M E; Wandersman, C

    1999-08-01

    The major mechanism by which bacteria acquire free or haemoglobin-bound haem involves direct binding of haem to specific outer membrane receptors. Serratia marcescens and Pseudomonas aeruginosa have an alternative system, which involves an extracellular haemophore, HasA, that captures free or haemoglobin-bound haem and shuttles it to a specific cell surface outer membrane receptor, HasR. Both haem-free (apoprotein) and haem-loaded (holoprotein) HasA bind to HasR, evidence for direct protein-protein interactions between HasA and HasR. HasA binding to HasR takes place in a tonB mutant. TonB is thus required for a step subsequent to HasA binding. PMID:10417645

  3. HASA: Hypersonic Aerospace Sizing Analysis for the Preliminary Design of Aerospace Vehicles

    NASA Technical Reports Server (NTRS)

    Harloff, Gary J.; Berkowitz, Brian M.

    1988-01-01

    A review of the hypersonic literature indicated that a general weight and sizing analysis was not available for hypersonic orbital, transport, and fighter vehicles. The objective here is to develop such a method for the preliminary design of aerospace vehicles. This report describes the developed methodology and provides examples to illustrate the model, entitled the Hypersonic Aerospace Sizing Analysis (HASA). It can be used to predict the size and weight of hypersonic single-stage and two-stage-to-orbit vehicles and transports, and is also relevant for supersonic transports. HASA is a sizing analysis that determines vehicle length and volume, consistent with body, fuel, structural, and payload weights. The vehicle component weights are obtained from statistical equations for the body, wing, tail, thermal protection system, landing gear, thrust structure, engine, fuel tank, hydraulic system, avionics, electral system, equipment payload, and propellant. Sample size and weight predictions are given for the Space Shuttle orbiter and other proposed vehicles, including four hypersonic transports, a Mach 6 fighter, a supersonic transport (SST), a single-stage-to-orbit (SSTO) vehicle, a two-stage Space Shuttle with a booster and an orbiter, and two methane-fueled vehicles.

  4. Hal Is a Bacillus anthracis heme acquisition protein.

    PubMed

    Balderas, Miriam A; Nobles, Christopher L; Honsa, Erin S; Alicki, Embriette R; Maresso, Anthony W

    2012-10-01

    The metal iron is a limiting nutrient for bacteria during infection. Bacillus anthracis, the causative agent of anthrax and a potential weapon of bioterrorism, grows rapidly in mammalian hosts, which suggests that it efficiently attains iron during infection. Recent studies have uncovered both heme (isd) and siderophore-mediated (asb) iron transport pathways in this pathogen. Whereas deletion of the asb genes results in reduced virulence, the loss of three surface components from isd had no effect, thereby leaving open the question of what additional factors in B. anthracis are responsible for iron uptake from the most abundant iron source for mammals, heme. Here, we describe the first functional characterization of bas0520, a gene recently implicated in anthrax disease progression. bas0520 encodes a single near-iron transporter (NEAT) domain and several leucine-rich repeats. The NEAT domain binds heme, despite lacking a stabilizing tyrosine common to the NEAT superfamily of hemoproteins. The NEAT domain also binds hemoglobin and can acquire heme from hemoglobin in solution. Finally, deletion of bas0520 resulted in bacilli unable to grow efficiently on heme or hemoglobin as an iron source and yielded the most significant phenotype relative to that for other putative heme uptake systems, a result that suggests that this protein plays a prominent role in the replication of B. anthracis in hematogenous environments. Thus, we have assigned the name of Hal (heme-acquisition leucine-rich repeat protein) to BAS0520. These studies advance our understanding of heme acquisition by this dangerous pathogen and justify efforts to determine the mechanistic function of this novel protein for vaccine or inhibitor development. PMID:22865843

  5. Protein Contribution to Plant Salinity Response and Tolerance Acquisition

    PubMed Central

    Kosová, Klára; Prášil, Ilja T.; Vítámvás, Pavel

    2013-01-01

    The review is focused on plant proteome response to salinity with respect to physiological aspects of plant salt stress response. The attention is paid to both osmotic and ionic effects of salinity stress on plants with respect to several protein functional groups. Therefore, the role of individual proteins involved in signalling, changes in gene expression, protein biosynthesis and degradation and the resulting changes in protein relative abundance in proteins involved in energy metabolism, redox metabolism, stressand defence-related proteins, osmolyte metabolism, phytohormone, lipid and secondary metabolism, mechanical stress-related proteins as well as protein posttranslational modifications are discussed. Differences between salt-sensitive (glycophytes) and salt-tolerant (halophytes) plants are analysed with respect to differential salinity tolerance. In conclusion, contribution of proteomic studies to understanding plant salinity tolerance is summarised and discussed. PMID:23531537

  6. Kinetic and spectroscopic studies of hemin acquisition in the hemophore HasAp from Pseudomonas aeruginosa.

    PubMed

    Yukl, Erik T; Jepkorir, Grace; Alontaga, Aileen Y; Pautsch, Lawrence; Rodriguez, Juan C; Rivera, Mario; Moënne-Loccoz, Pierre

    2010-08-10

    The extreme limitation of free iron has driven various pathogens to acquire iron from the host in the form of heme. Specifically, several Gram-negative pathogens secrete a heme binding protein known as HasA to scavenge heme from the extracellular environment and to transfer it to the receptor protein HasR for import into the bacterial cell. Structures of heme-bound and apo-HasA homologues show that the heme iron(III) ligands, His32 and Tyr75, reside on loops extending from the core of the protein and that a significant conformational change must occur at the His32 loop upon heme binding. Here, we investigate the kinetics of heme acquisition by HasA from Pseudomonas aeruginosa (HasAp). The rate of heme acquisition from human met-hemoglobin (met-Hb) closely matches that of heme dissociation which suggests a passive mode of heme uptake from this source. The binding of free hemin is characterized by an initial rapid phase forming an intermediate before further conversion to the final complex. Analysis of this same reaction using an H32A variant lacking the His heme ligand shows only the rapid phase to form a heme-protein complex spectroscopically equivalent to that of the wild-type intermediate. Further characterization of these reactions using electron paramagnetic resonance and resonance Raman spectroscopy of rapid freeze quench samples provides support for a model in which heme is initially bound by the Tyr75 to form a high-spin heme-protein complex before slower coordination of the His32 ligand upon closing of the His loop over the heme. The slow rate of this loop closure implies that the induced-fit mechanism of heme uptake in HasAp is not based on a rapid sampling of the H32 loop between open and closed configurations but, rather, that the H32 loop motions are triggered by the formation of the high-spin heme-HasAp intermediate complex. PMID:20586423

  7. Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes.

    PubMed

    Liu, Jianying; Liu, Yang; Nie, Kaixiao; Du, Senyan; Qiu, Jingjun; Pang, Xiaojing; Wang, Penghua; Cheng, Gong

    2016-01-01

    The arbovirus life cycle involves viral transfer between a vertebrate host and an arthropod vector, and acquisition of virus from an infected mammalian host by a vector is an essential step in this process. Here, we report that flavivirus nonstructural protein-1 (NS1), which is abundantly secreted into the serum of an infected host, plays a critical role in flavivirus acquisition by mosquitoes. The presence of dengue virus (DENV) and Japanese encephalitis virus NS1s in the blood of infected interferon-α and γ receptor-deficient mice (AG6) facilitated virus acquisition by their native mosquito vectors because the protein enabled the virus to overcome the immune barrier of the mosquito midgut. Active immunization of AG6 mice with a modified DENV NS1 reduced DENV acquisition by mosquitoes and protected mice against a lethal DENV challenge, suggesting that immunization with NS1 could reduce the number of virus-carrying mosquitoes as well as the incidence of flaviviral diseases. Our study demonstrates that flaviviruses utilize NS1 proteins produced during their vertebrate phases to enhance their acquisition by vectors, which might be a result of flavivirus evolution to adapt to multiple host environments. PMID:27562253

  8. Whole cell, label free protein quantitation with data independent acquisition: quantitation at the MS2 level.

    PubMed

    McQueen, Peter; Spicer, Vic; Schellenberg, John; Krokhin, Oleg; Sparling, Richard; Levin, David; Wilkins, John A

    2015-01-01

    Label free quantitation by measurement of peptide fragment signal intensity (MS2 quantitation) is a technique that has seen limited use due to the stochastic nature of data dependent acquisition (DDA). However, data independent acquisition has the potential to make large scale MS2 quantitation a more viable technique. In this study we used an implementation of data independent acquisition--SWATH--to perform label free protein quantitation in a model bacterium Clostridium stercorarium. Four tryptic digests analyzed by SWATH were probed by an ion library containing information on peptide mass and retention time obtained from DDA experiments. Application of this ion library to SWATH data quantified 1030 proteins with at least two peptides quantified (∼ 40% of predicted proteins in the C. stercorarium genome) in each replicate. Quantitative results obtained were very consistent between biological replicates (R(2) ∼ 0.960). Protein quantitation by summation of peptide fragment signal intensities was also highly consistent between biological replicates (R(2) ∼ 0.930), indicating that this approach may have increased viability compared to recent applications in label free protein quantitation. SWATH based quantitation was able to consistently detect differences in relative protein quantity and it provided coverage for a number of proteins that were missed in some samples by DDA analysis. PMID:25348682

  9. Influence of cobalamin scarcity on diatom molecular physiology and identification of a cobalamin acquisition protein.

    PubMed

    Bertrand, Erin M; Allen, Andrew E; Dupont, Christopher L; Norden-Krichmar, Trina M; Bai, Jing; Valas, Ruben E; Saito, Mak A

    2012-06-26

    Diatoms are responsible for ~40% of marine primary production and are key players in global carbon cycling. There is mounting evidence that diatom growth is influenced by cobalamin (vitamin B(12)) availability. This cobalt-containing micronutrient is only produced by some bacteria and archaea but is required by many diatoms and other eukaryotic phytoplankton. Despite its potential importance, little is known about mechanisms of cobalamin acquisition in diatoms or the impact of cobalamin scarcity on diatom molecular physiology. Proteomic profiling and RNA-sequencing transcriptomic analysis of the cultured diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana revealed three distinct strategies used by diatoms to cope with low cobalamin: increased cobalamin acquisition machinery, decreased cobalamin demand, and management of reduced methionine synthase activity through changes in folate and S-adenosyl methionine metabolism. One previously uncharacterized protein, cobalamin acquisition protein 1 (CBA1), was up to 160-fold more abundant under low cobalamin availability in both diatoms. Autologous overexpression of CBA1 revealed association with the outside of the cell and likely endoplasmic reticulum localization. Cobalamin uptake rates were elevated in strains overexpressing CBA1, directly linking this protein to cobalamin acquisition. CBA1 is unlike characterized cobalamin acquisition proteins and is the only currently identified algal protein known to be implicated in cobalamin uptake. The abundance and widespread distribution of transcripts encoding CBA1 in environmental samples suggests that cobalamin is an important nutritional factor for phytoplankton. Future study of CBA1 and other molecular signatures of cobalamin scarcity identified here will yield insight into the evolution of cobalamin utilization and facilitate monitoring of cobalamin starvation in oceanic diatom communities. PMID:22652568

  10. Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein.

    PubMed

    Silas, Sukrit; Mohr, Georg; Sidote, David J; Markham, Laura M; Sanchez-Amat, Antonio; Bhaya, Devaki; Lambowitz, Alan M; Fire, Andrew Z

    2016-02-26

    CRISPR systems mediate adaptive immunity in diverse prokaryotes. CRISPR-associated Cas1 and Cas2 proteins have been shown to enable adaptation to new threats in type I and II CRISPR systems by the acquisition of short segments of DNA (spacers) from invasive elements. In several type III CRISPR systems, Cas1 is naturally fused to a reverse transcriptase (RT). In the marine bacterium Marinomonas mediterranea (MMB-1), we showed that a RT-Cas1 fusion protein enables the acquisition of RNA spacers in vivo in a RT-dependent manner. In vitro, the MMB-1 RT-Cas1 and Cas2 proteins catalyze the ligation of RNA segments into the CRISPR array, which is followed by reverse transcription. These observations outline a host-mediated mechanism for reverse information flow from RNA to DNA. PMID:26917774

  11. Transcription of genes encoding iron and heme acquisition proteins of Haemophilus influenzae during acute otitis media.

    PubMed Central

    Whitby, P W; Sim, K E; Morton, D J; Patel, J A; Stull, T L

    1997-01-01

    Unencapsulated Haemophilus influenzae is the second most common etiologic agent of otitis media in children. H. influenzae requires heme for aerobic growth in vitro and is able to utilize hemoglobin and complexes of heme-hemopexin, heme-albumin, and hemoglobin-haptoglobin and ferritransferrin as sources of iron and heme in vitro. Several of the acquisition mechanisms have been characterized and been shown to be heme repressible in vitro. However, little is known about the expression of heme and/or iron acquisition mechanisms during infections in the middle ear. This study was performed to determine if the genes encoding heme and iron acquisition proteins are transcribed during in vivo growth and to compare these findings with those for samples grown in vitro. Reverse transcriptase PCR (RT-PCR) was used to analyze total RNA fractions derived from in vitro- and in vivo-grown H. influenzae. Genes encoding the transferrin-binding proteins TbpA and TbpB, the 100-kDa hemopexin-binding protein HxuA, and the hemoglobin-binding protein HgpA were transcribed during otitis media. Twelve middle ear fluid samples were analyzed by blind RT-PCR to determine the transcriptional status of these genes in H. influenzae during otitis media. Five isolates had transcripts corresponding to tbpA, tbpB, and hxuA. The presence of hgpA transcripts was variable, depending on the presence of hgpA in the genome of the H. influenzae isolate. Samples without H. influenzae gene transcripts contained other etiologic agents commonly causing otitis media. These data demonstrate that H. influenzae iron and/or heme acquisition genes are transcribed during otitis media and suggest that the microenvironment during acute otitis media starves H. influenzae of heme. PMID:9353052

  12. Arabinogalactan-protein secretion is associated with the acquisition of stigmatic receptivity in the apple flower

    PubMed Central

    Losada, Juan M.; Herrero, María

    2012-01-01

    Background and Aims Stigmatic receptivity plays a clear role in pollination dynamics; however, little is known about the factors that confer to a stigma the competence to be receptive for the germination of pollen grains. In this work, a developmental approach is used to evaluate the acquisition of stigmatic receptivity and its relationship with a possible change in arabinogalactan-proteins (AGPs). Methods Flowers of the domestic apple, Malus × domestica, were assessed for their capacity to support pollen germination at different developmental stages. Stigmas from these same stages were characterized morphologically and different AGP epitopes detected by immunocytochemistry. Key Results Acquisition of stigmatic receptivity and the secretion of classical AGPs from stigmatic cells occurred concurrently and following the same spatial distribution. While in unpollinated stigmas AGPs appeared unaltered, in cross-pollinated stigmas AGPs epitopes vanished as pollen tubes passed by. Conclusions The concurrent secretion of AGPs with the acquisition of stigmatic receptivity, together with the differential response in unpollinated and cross-pollinated pistils point out a role of AGPs in supporting pollen tube germination and strongly suggest that secretion of AGPs is associated with the acquisition of stigma receptivity. PMID:22652420

  13. Evolution of Helicobacter: Acquisition by Gastric Species of Two Histidine-Rich Proteins Essential for Colonization

    PubMed Central

    Vorontsov, Egor; Gallaud, Julien; Malosse, Christian; Michel, Valérie; Cavazza, Christine; Robbe-Saule, Marie; Richaud, Pierre; Chamot-Rooke, Julia; Brochier-Armanet, Céline; De Reuse, Hilde

    2015-01-01

    Metal acquisition and intracellular trafficking are crucial for all cells and metal ions have been recognized as virulence determinants in bacterial pathogens. Virulence of the human gastric pathogen Helicobacter pylori is dependent on nickel, cofactor of two enzymes essential for in vivo colonization, urease and [NiFe] hydrogenase. We found that two small paralogous nickel-binding proteins with high content in Histidine (Hpn and Hpn-2) play a central role in maintaining non-toxic intracellular nickel content and in controlling its intracellular trafficking. Measurements of metal resistance, intracellular nickel contents, urease activities and interactomic analysis were performed. We observed that Hpn acts as a nickel-sequestration protein, while Hpn-2 is not. In vivo, Hpn and Hpn-2 form homo-multimers, interact with each other, Hpn interacts with the UreA urease subunit while Hpn and Hpn-2 interact with the HypAB hydrogenase maturation proteins. In addition, Hpn-2 is directly or indirectly restricting urease activity while Hpn is required for full urease activation. Based on these data, we present a model where Hpn and Hpn-2 participate in a common pathway of controlled nickel transfer to urease. Using bioinformatics and top-down proteomics to identify the predicted proteins, we established that Hpn-2 is only expressed by H. pylori and its closely related species Helicobacter acinonychis. Hpn was detected in every gastric Helicobacter species tested and is absent from the enterohepatic Helicobacter species. Our phylogenomic analysis revealed that Hpn acquisition was concomitant with the specialization of Helicobacter to colonization of the gastric environment and the duplication at the origin of hpn-2 occurred in the common ancestor of H. pylori and H. acinonychis. Finally, Hpn and Hpn-2 were found to be required for colonization of the mouse model by H. pylori. Our data show that during evolution of the Helicobacter genus, acquisition of Hpn and Hpn-2 by gastric

  14. A Relay Network of Extracellular Heme-Binding Proteins Drives C. albicans Iron Acquisition from Hemoglobin

    PubMed Central

    Kuznets, Galit; Vigonsky, Elena; Weissman, Ziva; Lalli, Daniela; Gildor, Tsvia; Kauffman, Sarah J.; Turano, Paola; Becker, Jeffrey; Lewinson, Oded; Kornitzer, Daniel

    2014-01-01

    Iron scavenging constitutes a crucial challenge for survival of pathogenic microorganisms in the iron-poor host environment. Candida albicans, like many microbial pathogens, is able to utilize iron from hemoglobin, the largest iron pool in the host's body. Rbt5 is an extracellular glycosylphosphatidylinositol (GPI)-anchored heme-binding protein of the CFEM family that facilitates heme-iron uptake by an unknown mechanism. Here, we characterize an additional C. albicans CFEM protein gene, PGA7, deletion of which elicits a more severe heme-iron utilization phenotype than deletion of RBT5. The virulence of the pga7−/− mutant is reduced in a mouse model of systemic infection, consistent with a requirement for heme-iron utilization for C. albicans pathogenicity. The Pga7 and Rbt5 proteins exhibit distinct cell wall attachment, and discrete localization within the cell envelope, with Rbt5 being more exposed than Pga7. Both proteins are shown here to efficiently extract heme from hemoglobin. Surprisingly, while Pga7 has a higher affinity for heme in vitro, we find that heme transfer can occur bi-directionally between Pga7 and Rbt5, supporting a model in which they cooperate in a heme-acquisition relay. Together, our data delineate the roles of Pga7 and Rbt5 in a cell surface protein network that transfers heme from extracellular hemoglobin to the endocytic pathway, and provide a paradigm for how receptors embedded in the cell wall matrix can mediate nutrient uptake across the fungal cell envelope. PMID:25275454

  15. Identification of phosphorus deficiency responsive proteins in a high phosphorus acquisition soybean (Glycine max) cultivar through proteomic analysis.

    PubMed

    Sha, Aihua; Li, Ming; Yang, Pingfang

    2016-05-01

    As one of the major oil crops, soybean might be seriously affected by phosphorus deficiency on both yield and quality. Understanding the molecular basis of phosphorus uptake and utilization in soybean may help to develop phosphorus (P) efficient cultivars. On this purpose, we conducted a comparative proteomic analysis on a high P acquisition soybean cultivar BX10 under low and high P conditions. A total of 61 unique proteins were identified as putative P deficiency responsive proteins. These proteins were involved in carbohydrate metabolism, protein biosynthesis/processing, energy metabolism, cellular processes, environmental defense/interaction, nucleotide metabolism, signal transduction, secondary metabolism and other metabolism related processes. Several proteins involved in energy metabolism, cellular processes, and protein biosynthesis and processing were found to be up-regulated in both shoots and roots, whereas, proteins involved in carbohydrate metabolism appeared to be down-regulated. These proteins are potential candidates for improving P acquisition. These findings provide a useful starting point for further research that will provide a more comprehensive understanding of molecular mechanisms through which soybeans adapt to P deficiency condition. PMID:26853500

  16. Regulatory protein BBD18 of the lyme disease spirochete: essential role during tick acquisition?

    PubMed

    Hayes, Beth M; Dulebohn, Daniel P; Sarkar, Amit; Tilly, Kit; Bestor, Aaron; Ambroggio, Xavier; Rosa, Patricia A

    2014-01-01

    The Lyme disease spirochete Borrelia burgdorferi senses and responds to environmental cues as it transits between the tick vector and vertebrate host. Failure to properly adapt can block transmission of the spirochete and persistence in either vector or host. We previously identified BBD18, a novel plasmid-encoded protein of B. burgdorferi, as a putative repressor of the host-essential factor OspC. In this study, we investigate the in vivo role of BBD18 as a regulatory protein, using an experimental mouse-tick model system that closely resembles the natural infectious cycle of B. burgdorferi. We show that spirochetes that have been engineered to constitutively produce BBD18 can colonize and persist in ticks but do not infect mice when introduced by either tick bite or needle inoculation. Conversely, spirochetes lacking BBD18 can persistently infect mice but are not acquired by feeding ticks. Through site-directed mutagenesis, we have demonstrated that abrogation of spirochete infection in mice by overexpression of BBD18 occurs only with bbd18 alleles that can suppress OspC synthesis. Finally, we demonstrate that BBD18-mediated regulation does not utilize a previously described ospC operator sequence required by B. burgdorferi for persistence in immunocompetent mice. These data lead us to conclude that BBD18 does not represent the putative repressor utilized by B. burgdorferi for the specific downregulation of OspC in the mammalian host. Rather, we suggest that BBD18 exhibits features more consistent with those of a global regulatory protein whose critical role occurs during spirochete acquisition by feeding ticks. IMPORTANCE Lyme disease, caused by Borrelia burgdorferi, is the most common arthropod-borne disease in North America. B. burgdorferi is transmitted to humans and other vertebrate hosts by ticks as they take a blood meal. Transmission between vectors and hosts requires the bacterium to sense changes in the environment and adapt. However, the mechanisms

  17. Rapid acquisition of multidimensional solid-state NMR spectra of proteins facilitated by covalently bound paramagnetic tags.

    PubMed

    Nadaud, Philippe S; Helmus, Jonathan J; Sengupta, Ishita; Jaroniec, Christopher P

    2010-07-21

    We describe a condensed data collection approach that facilitates rapid acquisition of multidimensional magic-angle spinning solid-state nuclear magnetic resonance (SSNMR) spectra of proteins by combining rapid sample spinning, optimized low-power radio frequency pulse schemes and covalently attached paramagnetic tags to enhance protein (1)H spin-lattice relaxation. Using EDTA-Cu(2+)-modified K28C and N8C mutants of the B1 immunoglobulin binding domain of protein G as models, we demonstrate that high resolution and sensitivity 2D and 3D SSNMR chemical shift correlation spectra can be recorded in as little as several minutes and several hours, respectively, for samples containing approximately 0.1-0.2 micromol of (13)C,(15)N- or (2)H,(13)C,(15)N-labeled protein. This mode of data acquisition is naturally suited toward the structural SSNMR studies of paramagnetic proteins, for which the typical (1)H longitudinal relaxation time constants are inherently a factor of at least approximately 3-4 lower relative to their diamagnetic counterparts. To illustrate this, we demonstrate the rapid site-specific determination of backbone amide (15)N longitudinal paramagnetic relaxation enhancements using a pseudo-3D SSNMR experiment based on (15)N-(13)C correlation spectroscopy, and we show that such measurements yield valuable long-range (15)N-Cu(2+) distance restraints which report on the three-dimensional protein fold. PMID:20583834

  18. Parallel data acquisition of in-source fragmented glycopeptides to sequence the glycosylation sites of proteins.

    PubMed

    Zhao, Jingfu; Song, Ehwang; Zhu, Rui; Mechref, Yehia

    2016-06-01

    Glycosylation plays important roles in maintaining protein stability and controlling biological processes. In recent years, the correlation between aberrant glycoproteins and many diseases has been reported. Hence, qualitative and quantitative analyses of glycoproteins are necessary to understand physiological processes. LC-MS/MS analysis of glycopeptides is faced with the low glycopeptide signal intensities and low peptide sequence identification. In our study, in-source fragmentation (ISF) was used in conjunction with LC-MS/MS to facilitate the parallel acquisition of peptide backbone sequence and glycan composition information. In ISF method, the identification of glycosylation sites depended on the detection of Y1 ion (ion of peptide backbone with an N-acetylglucosamine attached). To attain dominant Y1 ions, a range of source fragmentation voltages was studied using fetuin. A 45 V ISF voltage was found to be the most efficient voltage for the analysis of glycoproteins. ISF was employed to study the glycosylation sites of three model glycoproteins, including fetuin, α1-acid glycoprotein and porcine thyroglobulin. The approach was then used to analyze blood serum samples. Y1 ions of glycopeptides in tryptic digests of samples were detected. Y1 ions of glycopeptides with different sialic acid groups are observed at different retention times, representing the various numbers of sialic acid moieties associated with the same peptide backbone sequence. With ISF facilitating the peptide backbone sequencing of glycopeptides, identified peptide sequence coverage was increased. For example, identified fetuin sequence percentage was improved from 39 to 80% in MASCOT database searching compared to conventional CID method. The formation of Y1 ions and oxonium ions in ISF facilitates glycopeptide sequencing and glycan composition identification. PMID:26957414

  19. Advancing Urinary Protein Biomarker Discovery by Data-Independent Acquisition on a Quadrupole-Orbitrap Mass Spectrometer.

    PubMed

    Muntel, Jan; Xuan, Yue; Berger, Sebastian T; Reiter, Lukas; Bachur, Richard; Kentsis, Alex; Steen, Hanno

    2015-11-01

    The promises of data-independent acquisition (DIA) strategies are a comprehensive and reproducible digital qualitative and quantitative record of the proteins present in a sample. We developed a fast and robust DIA method for comprehensive mapping of the urinary proteome that enables large scale urine proteomics studies. Compared to a data-dependent acquisition (DDA) experiments, our DIA assay doubled the number of identified peptides and proteins per sample at half the coefficients of variation observed for DDA data (DIA = ∼8%; DDA = ∼16%). We also tested different spectral libraries and their effects on overall protein and peptide identifications and their reproducibilities, which provided clear evidence that sample type-specific spectral libraries are preferred for reliable data analysis. To show applicability for biomarker discovery experiments, we analyzed a sample set of 87 urine samples from children seen in the emergency department with abdominal pain. The whole set was analyzed with high proteome coverage (∼1300 proteins/sample) in less than 4 days. The data set revealed excellent biomarker candidates for ovarian cyst and urinary tract infection. The improved throughput and quantitative performance of our optimized DIA workflow allow for the efficient simultaneous discovery and verification of biomarker candidates without the requirement for an early bias toward selected proteins. PMID:26423119

  20. MavN is a Legionella pneumophila vacuole-associated protein required for efficient iron acquisition during intracellular growth

    PubMed Central

    Isaac, Dervla T.; Laguna, Rita K.; Valtz, Nicole; Isberg, Ralph R.

    2015-01-01

    Iron is essential for the growth and virulence of most intravacuolar pathogens. The mechanisms by which microbes bypass host iron restriction to gain access to this metal across the host vacuolar membrane are poorly characterized. In this work, we identify a unique intracellular iron acquisition strategy used by Legionella pneumophila. The bacterial Icm/Dot (intracellular multiplication/defect in organelle trafficking) type IV secretion system targets the bacterial-derived MavN (more regions allowing vacuolar colocalization N) protein to the surface of the Legionella-containing vacuole where this putative transmembrane protein facilitates intravacuolar iron acquisition. The ΔmavN mutant exhibits a transcriptional iron-starvation signature before its growth is arrested during the very early stages of macrophage infection. This intracellular growth defect is rescued only by the addition of excess exogenous iron to the culture medium and not a variety of other metals. Consistent with MavN being a translocated substrate that plays an exclusive role during intracellular growth, the mutant shows no defect for growth in broth culture, even under severe iron-limiting conditions. Putative iron-binding residues within the MavN protein were identified, and point mutations in these residues resulted in defects specific for intracellular growth that are indistinguishable from the ΔmavN mutant. This model of a bacterial protein inserting into host membranes to mediate iron transport provides a paradigm for how intravacuolar pathogens can use virulence-associated secretion systems to manipulate and acquire host iron. PMID:26330609

  1. MavN is a Legionella pneumophila vacuole-associated protein required for efficient iron acquisition during intracellular growth.

    PubMed

    Isaac, Dervla T; Laguna, Rita K; Valtz, Nicole; Isberg, Ralph R

    2015-09-15

    Iron is essential for the growth and virulence of most intravacuolar pathogens. The mechanisms by which microbes bypass host iron restriction to gain access to this metal across the host vacuolar membrane are poorly characterized. In this work, we identify a unique intracellular iron acquisition strategy used by Legionella pneumophila. The bacterial Icm/Dot (intracellular multiplication/defect in organelle trafficking) type IV secretion system targets the bacterial-derived MavN (more regions allowing vacuolar colocalization N) protein to the surface of the Legionella-containing vacuole where this putative transmembrane protein facilitates intravacuolar iron acquisition. The ΔmavN mutant exhibits a transcriptional iron-starvation signature before its growth is arrested during the very early stages of macrophage infection. This intracellular growth defect is rescued only by the addition of excess exogenous iron to the culture medium and not a variety of other metals. Consistent with MavN being a translocated substrate that plays an exclusive role during intracellular growth, the mutant shows no defect for growth in broth culture, even under severe iron-limiting conditions. Putative iron-binding residues within the MavN protein were identified, and point mutations in these residues resulted in defects specific for intracellular growth that are indistinguishable from the ΔmavN mutant. This model of a bacterial protein inserting into host membranes to mediate iron transport provides a paradigm for how intravacuolar pathogens can use virulence-associated secretion systems to manipulate and acquire host iron. PMID:26330609

  2. IlsA, A Unique Surface Protein of Bacillus cereus Required for Iron Acquisition from Heme, Hemoglobin and Ferritin

    PubMed Central

    Daou, Nadine; Buisson, Christophe; Gohar, Michel; Vidic, Jasmina; Bierne, Hélène; Kallassy, Mireille; Lereclus, Didier; Nielsen-LeRoux, Christina

    2009-01-01

    The human opportunistic pathogen Bacillus cereus belongs to the B. cereus group that includes bacteria with a broad host spectrum. The ability of these bacteria to colonize diverse hosts is reliant on the presence of adaptation factors. Previously, an IVET strategy led to the identification of a novel B. cereus protein (IlsA, Iron-regulated leucine rich surface protein), which is specifically expressed in the insect host or under iron restrictive conditions in vitro. Here, we show that IlsA is localized on the surface of B. cereus and hence has the potential to interact with host proteins. We report that B. cereus uses hemoglobin, heme and ferritin, but not transferrin and lactoferrin. In addition, affinity tests revealed that IlsA interacts with both hemoglobin and ferritin. Furthermore, IlsA directly binds heme probably through the NEAT domain. Inactivation of ilsA drastically decreases the ability of B. cereus to grow in the presence of hemoglobin, heme and ferritin, indicating that IlsA is essential for iron acquisition from these iron sources. In addition, the ilsA mutant displays a reduction in growth and virulence in an insect model. Hence, our results indicate that IlsA is a key factor within a new iron acquisition system, playing an important role in the general virulence strategy adapted by B. cereus to colonize susceptible hosts. PMID:19956654

  3. Evolution of photorespiration from cyanobacteria to land plants, considering protein phylogenies and acquisition of carbon concentrating mechanisms.

    PubMed

    Hagemann, Martin; Kern, Ramona; Maurino, Veronica G; Hanson, David T; Weber, Andreas P M; Sage, Rowan F; Bauwe, Hermann

    2016-05-01

    Photorespiration and oxygenic photosynthesis are intimately linked processes. It has been shown that under the present day atmospheric conditions cyanobacteria and all eukaryotic phototrophs need functional photorespiration to grow autotrophically. The question arises as to when this essential partnership evolved, i.e. can we assume a coevolution of both processes from the beginning or did photorespiration evolve later to compensate for the generation of 2-phosphoglycolate (2PG) due to Rubisco's oxygenase reaction? This question is mainly discussed here using phylogenetic analysis of proteins involved in the 2PG metabolism and the acquisition of different carbon concentrating mechanisms (CCMs). The phylogenies revealed that the enzymes involved in the photorespiration of vascular plants have diverse origins, with some proteins acquired from cyanobacteria as ancestors of the chloroplasts and others from heterotrophic bacteria as ancestors of mitochondria in the plant cell. Only phosphoglycolate phosphatase was found to originate from Archaea. Notably glaucophyte algae, the earliest branching lineage of Archaeplastida, contain more photorespiratory enzymes of cyanobacterial origin than other algal lineages or land plants indicating a larger initial contribution of cyanobacterial-derived proteins to eukaryotic photorespiration. The acquisition of CCMs is discussed as a proxy for assessing the timing of periods when photorespiratory activity may have been enhanced. The existence of CCMs also had marked influence on the structure and function of photorespiration. Here, we discuss evidence for an early and continuous coevolution of photorespiration, CCMs and photosynthesis starting from cyanobacteria via algae, to land plants. PMID:26931168

  4. Acquisition of heme iron by Neisseria meningitidis does not involve meningococcal transferrin-binding proteins.

    PubMed

    Martel, N; Lee, B C

    1994-02-01

    Similarities in size between hemin-binding protein 1 (HmBP1) and transferrin-binding protein 1 (TBP1) of Neisseria meningitidis suggest that these proteins are functionally homologous. However, a meningococcal mutant lacking the transferrin-binding proteins retained the capacity to acquire iron from heme and hemoglobin. In immunoblots, hyperimmune polyclonal antiserum against TBP1 did not react with HmBP1. PMID:8300227

  5. Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

    PubMed Central

    Booth, David S.; Avila-Sakar, Agustin; Cheng, Yifan

    2011-01-01

    Single particle electron microscopy (EM), of both negative stained or frozen hydrated biological samples, has become a versatile tool in structural biology 1. In recent years, this method has achieved great success in studying structures of proteins and macromolecular complexes 2, 3. Compared with electron cryomicroscopy (cryoEM), in which frozen hydrated protein samples are embedded in a thin layer of vitreous ice 4, negative staining is a simpler sample preparation method in which protein samples are embedded in a thin layer of dried heavy metal salt to increase specimen contrast 5. The enhanced contrast of negative stain EM allows examination of relatively small biological samples. In addition to determining three-dimensional (3D) structure of purified proteins or protein complexes 6, this method can be used for much broader purposes. For example, negative stain EM can be easily used to visualize purified protein samples, obtaining information such as homogeneity/heterogeneity of the sample, formation of protein complexes or large assemblies, or simply to evaluate the quality of a protein preparation. In this video article, we present a complete protocol for using an EM to observe negatively stained protein sample, from preparing carbon coated grids for negative stain EM to acquiring images of negatively stained sample in an electron microscope operated at 120kV accelerating voltage. These protocols have been used in our laboratory routinely and can be easily followed by novice users. PMID:22215030

  6. Simultaneous Acquisition of 2D and 3D Solid-State NMR Experiments for Sequential Assignment of Oriented Membrane Protein Samples

    PubMed Central

    Gopinath, T.; Mote, Kaustubh R; Veglia, Gianluigi

    2016-01-01

    We present a new method called DAISY (Dual Acquisition orIented ssNMR spectroScopY) for the simultaneous acquisition of 2D and 3D oriented solid-state NMR experiments for membrane proteins aligned in mechanically or magnetically lipid bilayers. DAISY utilizes dual acquisition of sine and cosine dipolar or chemical shift coherences and long living 15N longitudinal polarization to obtain two multi-dimensional spectra, simultaneously. In these new experiments, the first acquisition gives the polarization inversion spin exchange at the magic angle (PISEMA) or heteronuclear correlation (HETCOR) spectra, the second acquisition gives PISEMA-mixing or HETCOR-mixing spectra, where the mixing element enables inter-residue correlations through 15N-15N homonuclear polarization transfer. The analysis of the two 2D spectra (first and second acquisitions) enables one to distinguish 15N-15N inter-residue correlations for sequential assignment of membrane proteins. DAISY can be implemented in 3D experiments that include the polarization inversion spin exchange at magic angle via I spin coherence (PISEMAI) sequence, as we show for the simultaneous acquisition of 3D PISEMAI-HETCOR and 3D PISEMAI-HETCOR-mixing experiments. PMID:25749871

  7. Simultaneous acquisition of 2D and 3D solid-state NMR experiments for sequential assignment of oriented membrane protein samples.

    PubMed

    Gopinath, T; Mote, Kaustubh R; Veglia, Gianluigi

    2015-05-01

    We present a new method called DAISY (Dual Acquisition orIented ssNMR spectroScopY) for the simultaneous acquisition of 2D and 3D oriented solid-state NMR experiments for membrane proteins reconstituted in mechanically or magnetically aligned lipid bilayers. DAISY utilizes dual acquisition of sine and cosine dipolar or chemical shift coherences and long living (15)N longitudinal polarization to obtain two multi-dimensional spectra, simultaneously. In these new experiments, the first acquisition gives the polarization inversion spin exchange at the magic angle (PISEMA) or heteronuclear correlation (HETCOR) spectra, the second acquisition gives PISEMA-mixing or HETCOR-mixing spectra, where the mixing element enables inter-residue correlations through (15)N-(15)N homonuclear polarization transfer. The analysis of the two 2D spectra (first and second acquisitions) enables one to distinguish (15)N-(15)N inter-residue correlations for sequential assignment of membrane proteins. DAISY can be implemented in 3D experiments that include the polarization inversion spin exchange at magic angle via I spin coherence (PISEMAI) sequence, as we show for the simultaneous acquisition of 3D PISEMAI-HETCOR and 3D PISEMAI-HETCOR-mixing experiments. PMID:25749871

  8. ACQUISITION AND LOSS OF NEURONAL CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE DURING NEURONAL DIFFERENTIATION

    EPA Science Inventory

    Neurons display characteristic schedules by which they acquire and lose the neuron-specific Ca2+/calmodulin-dependent protein Kinase-Gr (CaM Kinase-Gr) during differentiation. uch schedules are exemplified by patterns of expression of this kinase in the developing cerebellum and ...

  9. Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics

    PubMed Central

    2012-01-01

    Background Precise matching between motoneuron subtypes and the muscles they innervate is a prerequisite for normal behavior. Motoneuron subtype identity is specified by the combination of transcription factors expressed by the cell during its differentiation. Here we investigate the roles of Mnx family transcription factors in specifying the subtypes of individually identified zebrafish primary motoneurons. Results Zebrafish has three Mnx family members. We show that each of them has a distinct and temporally dynamic expression pattern in each primary motoneuron subtype. We also show that two Mnx family members are expressed in identified VeLD interneurons derived from the same progenitor domain that generates primary motoneurons. Surprisingly, we found that Mnx proteins appear unnecessary for differentiation of VeLD interneurons or the CaP motoneuron subtype. Mnx proteins are, however, required for differentiation of the MiP motoneuron subtype. We previously showed that MiPs require two temporally-distinct phases of Islet1 expression for normal development. Here we show that in the absence of Mnx proteins, the later phase of Islet1 expression is initiated but not sustained, and MiPs become hybrids that co-express morphological and molecular features of motoneurons and V2a interneurons. Unexpectedly, these hybrid MiPs often extend CaP-like axons, and some MiPs appear to be entirely transformed to a CaP morphology. Conclusions Our results suggest that Mnx proteins promote MiP subtype identity by suppressing both interneuron development and CaP axon pathfinding. This is, to our knowledge, the first report of transcription factors that act to distinguish CaP and MiP subtype identities. Our results also suggest that MiP motoneurons are more similar to V2 interneurons than are CaP motoneurons. PMID:23122226

  10. Membrane Anchoring of Aminoacyl-tRNA Synthetases by Convergent Acquisition of a Novel Protein Domain*

    PubMed Central

    Olmedo-Verd, Elvira; Santamaría-Gómez, Javier; Ochoa de Alda, Jesús A. G.; Ribas de Pouplana, Lluis; Luque, Ignacio

    2011-01-01

    Four distinct aminoacyl-tRNA synthetases (aaRSs) found in some cyanobacterial species contain a novel protein domain that bears two putative transmembrane helices. This CAAD domain is present in glutamyl-, isoleucyl-, leucyl-, and valyl-tRNA synthetases, the latter of which has probably recruited the domain more than once during evolution. Deleting the CAAD domain from the valyl-tRNA synthetase of Anabaena sp. PCC 7120 did not significantly modify the catalytic properties of this enzyme, suggesting that it does not participate in its canonical tRNA-charging function. Multiple lines of evidence suggest that the function of the CAAD domain is structural, mediating the membrane anchorage of the enzyme, although membrane localization of aaRSs has not previously been described in any living organism. Synthetases containing the CAAD domain were localized in the intracytoplasmic thylakoid membranes of cyanobacteria and were largely absent from the plasma membrane. The CAAD domain was necessary and apparently sufficient for protein targeting to membranes. Moreover, localization of aaRSs in thylakoids was important under nitrogen limiting conditions. In Anabaena, a multicellular filamentous cyanobacterium often used as a model for prokaryotic cell differentiation, valyl-tRNA synthetase underwent subcellular relocation at the cell poles during heterocyst differentiation, a process also dependent on the CAAD domain. PMID:21965654

  11. Microsecond acquisition of heterogeneous structure in the folding of a TIM barrel protein

    SciTech Connect

    Wu, Ying; Kondrashkina, Elena; Kayatekin, Can; Matthews, C. Robert; Bilsel, Osman

    2008-09-29

    The earliest kinetic folding events for ({beta}{alpha}){sub 8} barrels reflect the appearance of off-pathway intermediates. Continuous-flow microchannel mixing methods interfaced to small-angle x-ray scattering (SAXS), circular dichroism (CD), time-resolved Foerster resonant energy transfer (trFRET), and time-resolved fluorescence anisotropy (trFLAN) have been used to directly monitor global and specific dimensional properties of the partially folded state in the microsecond time range for a representative ({beta}{alpha}){sub 8} barrel protein. Within 150 {micro}s, the {alpha}-subunit of Trp synthase ({alpha}TS) experiences a global collapse and the partial formation of secondary structure. The time resolution of the folding reaction was enhanced with trFRET and trFLAN to show that, within 30 {micro}s, a distinct and autonomous partially collapsed structure has already formed in the N-terminal and central regions but not in the C-terminal region. A distance distribution analysis of the trFRET data confirmed the presence of a heterogeneous ensemble that persists for several hundreds of microseconds. Ready access to locally folded, stable substructures may be a hallmark of repeat-module proteins and the source of early kinetic traps in these very common motifs. Their folding free-energy landscapes should be elaborated to capture this source of frustration.

  12. Multiple Acquisition of Magic Angle Spinning Solid-State NMR Experiments Using One Receiver: Application to Microcrystalline and Membrane Protein Preparations

    PubMed Central

    Gopinath, T.; Veglia, Gianluigi

    2015-01-01

    Solid-State NMR spectroscopy of proteins is a notoriously low-throughput technique. Relatively low-sensitivity and poor resolution of protein samples require long acquisition times for multidimensional NMR experiments. To speed up data acquisition, we developed a family of experiments called Polarization Optimized Experiments (POE), in which we utilized the orphan spin operators that are discarded in classical multidimensional NMR experiments, recovering them to allow simultaneous acquisition of multiple 2D and 3D experiments, all while using conventional probes with spectrometers equipped with one receiver. POEs allow the concatenation of multiple 2D or 3D pulse sequences into a single experiment, thus potentially combining all of the aforementioned advances, boosting the capability of ssNMR spectrometers at least two-fold without the addition of any hardware. In this Perspective, we describe the first generation of POEs, such as dual acquisition MAS (or DUMAS) methods, and then illustrate the evolution of these experiments into MEIOSIS, a method that enables the simultaneous acquisition of multiple 2D and 3D spectra. Using these new pulse schemes for the solid-state NMR investigation of biopolymers makes it possible to obtain sequential resonance assignments, as well as distance restraints, in about half the experimental time. While designed for acquisition of heteronuclei, these new experiments can be easily implemented for proton detection and coupled with other recent advancements, such as dynamic polarization, to improve signal to noise. Finally, we illustrate the application of these methods to microcrystalline protein preparations as well as single and multi-span membrane proteins reconstituted in lipid membranes. PMID:25797011

  13. Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: Application to microcrystalline and membrane protein preparations

    NASA Astrophysics Data System (ADS)

    Gopinath, T.; Veglia, Gianluigi

    2015-04-01

    Solid-state NMR spectroscopy of proteins is a notoriously low-throughput technique. Relatively low-sensitivity and poor resolution of protein samples require long acquisition times for multidimensional NMR experiments. To speed up data acquisition, we developed a family of experiments called Polarization Optimized Experiments (POE), in which we utilized the orphan spin operators that are discarded in classical multidimensional NMR experiments, recovering them to allow simultaneous acquisition of multiple 2D and 3D experiments, all while using conventional probes with spectrometers equipped with one receiver. POE allow the concatenation of multiple 2D or 3D pulse sequences into a single experiment, thus potentially combining all of the aforementioned advances, boosting the capability of ssNMR spectrometers at least two-fold without the addition of any hardware. In this perspective, we describe the first generation of POE, such as dual acquisition MAS (or DUMAS) methods, and then illustrate the evolution of these experiments into MEIOSIS, a method that enables the simultaneous acquisition of multiple 2D and 3D spectra. Using these new pulse schemes for the solid-state NMR investigation of biopolymers makes it possible to obtain sequential resonance assignments, as well as distance restraints, in about half the experimental time. While designed for acquisition of heteronuclei, these new experiments can be easily implemented for proton detection and coupled with other recent advancements, such as dynamic nuclear polarization (DNP), to improve signal to noise. Finally, we illustrate the application of these methods to microcrystalline protein preparations as well as single and multi-span membrane proteins reconstituted in lipid membranes.

  14. Effect of varied gestational stress on acquisition of spatial memory, hippocampal LTP and synaptic proteins in juvenile male rats.

    PubMed

    Yaka, Rami; Salomon, Shiri; Matzner, Henry; Weinstock, Marta

    2007-04-16

    Some but not other forms of prenatal stress have been shown to impair spatial memory in adult male offspring. It is not clear if this is because of the intensity of the stress, age of rats, or the way in which learning is assessed. We examined the effect of daily varied prenatal stress consisting of 30 min restraint, saline injections and 15 min forced swim on day 17-21 of gestation on spatial learning, synaptic plasticity and the expression of key proteins of the post synaptic density (PSD) in the hippocampus of males aged 4-5 weeks. Prenatal stress impaired spatial learning in the Morris water maze and induced a significant decrease in long-term potentiation (LTP) in hippocampal slices. There was no change in the paired pulse facilitation ratio but there was a significant reduction in the expression of the NR2B subunit of the glutamate type NMDA receptor and the GluR1 subunit of the AMPA receptor, both of which are important modulators of LTP. These changes were accompanied by a remarkable increase in the scaffolding protein PSD95, which interacts with the intracellular carboxy terminal domains of the NR2 subunits. The high levels of PSD95 may have contributed to the impairment of LTP by disrupting the clustering of NMDA receptors in CA1 synapses. The alteration by prenatal stress in the relative amounts of scaffolding proteins and those which compose glutamate receptors could explain the depression of LTP and impairment in the acquisition of spatial learning. PMID:17320196

  15. Cryptococcus neoformans Requires the ESCRT Protein Vps23 for Iron Acquisition from Heme, for Capsule Formation, and for Virulence

    PubMed Central

    Hu, Guanggan; Caza, Mélissa; Cadieux, Brigitte; Chan, Vivienne; Liu, Victor

    2013-01-01

    Iron availability is a key regulator of virulence factor elaboration in Cryptococcus neoformans, the causative agent of fungal meningoencephalitis in HIV/AIDS patients. In addition, iron is an essential nutrient for pathogen proliferation in mammalian hosts but little is known about the mechanisms of iron sensing and uptake in fungal pathogens that attack humans. In this study, we mutagenized C. neoformans by Agrobacterium-mediated T-DNA insertion and screened for mutants with reduced growth on heme as the sole iron source. Among 34 mutants, we identified a subset with insertions in the gene for the ESCRT-I (endosomal sorting complex required for transport) protein Vps23 that resulted in a growth defect on heme, presumably due to a defect in uptake via endocytosis or misregulation of iron acquisition from heme. Remarkably, vps23 mutants were also defective in the elaboration of the cell-associated capsular polysaccharide that is a major virulence factor, while overexpression of Vps23 resulted in cells with a slightly enlarged capsule. These phenotypes were mirrored by a virulence defect in the vps23 mutant in a mouse model of cryptococcosis and by hypervirulence of the overexpression strain. Overall, these results reveal an important role for trafficking via ESCRT functions in both heme uptake and capsule formation, and they further reinforce the connection between iron and virulence factor deployment in C. neoformans. PMID:23132495

  16. Application of wide selected-ion monitoring data-independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor.

    PubMed

    Martin, Laetitia B B; Sherwood, Robert W; Nicklay, Joshua J; Yang, Yong; Muratore-Schroeder, Tara L; Anderson, Elizabeth T; Thannhauser, Theodore W; Rose, Jocelyn K C; Zhang, Sheng

    2016-08-01

    We describe here the use of label-free wide selected-ion monitoring data-independent acquisition (WiSIM-DIA) to identify proteins that are involved in the formation of tomato (Solanum lycopersicum) fruit cuticles and that are regulated by the transcription factor CUTIN DEFICIENT2 (CD2). A spectral library consisting of 11 753 unique peptides, corresponding to 2338 tomato protein groups, was used and the DIA analysis was performed at the MS1 level utilizing narrow mass windows for extraction with Skyline 2.6 software. We identified a total of 1140 proteins, 67 of which had expression levels that differed significantly between the cd2 tomato mutant and the wild-type cultivar M82. Differentially expressed proteins including a key protein involved in cutin biosynthesis, were selected for validation by target SRM/MRM and by Western blot analysis. In addition to confirming a role for CD2 in regulating cuticle formation, the results also revealed that CD2 influences pathways associated with cell wall biology, anthocyanin biosynthesis, plant development, and responses to stress, which complements findings of earlier RNA-Seq experiments. Our results provide new insights into molecular processes and aspects of fruit biology associated with CD2 function, and demonstrate that the WiSIM-DIA is an effective quantitative approach for global protein identifications. PMID:27089858

  17. In-depth protein profiling of the postsynaptic density from mouse hippocampus using data-independent acquisition proteomics.

    PubMed

    Distler, Ute; Schmeisser, Michael J; Pelosi, Assunta; Reim, Dominik; Kuharev, Jörg; Weiczner, Roland; Baumgart, Jan; Boeckers, Tobias M; Nitsch, Robert; Vogt, Johannes; Tenzer, Stefan

    2014-11-01

    Located at neuronal terminals, the postsynaptic density (PSD) is a highly complex network of cytoskeletal scaffolding and signaling proteins responsible for the transduction and modulation of glutamatergic signaling between neurons. Using ion-mobility enhanced data-independent label-free LC-MS/MS, we established a reference proteome of crude synaptosomes, synaptic junctions, and PSD derived from mouse hippocampus including TOP3-based absolute quantification values for identified proteins. The final dataset across all fractions comprised 49 491 peptides corresponding to 4558 protein groups. Of these, 2102 protein groups were identified in highly purified PSD in at least two biological replicates. Identified proteins play pivotal roles in neurological and synaptic processes providing a rich resource for studies on hippocampal PSD function as well as on the pathogenesis of neuropsychiatric disorders. All MS data have been deposited in the ProteomeXchange with identifier PXD000590 (http://proteomecentral.proteomexchange.org/dataset/PXD000590). PMID:25211037

  18. Acquisition of thermotolerance in soybean seedlings: synthesis and accumulation of heat shock proteins and their cellular localization

    SciTech Connect

    Lin, C.Y.; Roberts, J.K.; Key, J.L.

    1984-01-01

    When soybean Glycine max var Wayne seedlings are shifted from a normal growth temperature of 28/sup 0/C up to 40/sup 0/C (heat shock or HS), there is a dramatic change in protein synthesis. A new set of proteins known as shock proteins (HSPs) is produced and normal protein synthesis is greatly reduced. However, a pretreatmemt at 40/sup 0/C or a brief (10 minute) pulse treatment at 45/sup 0/C followed by a 28/sup 0/C incubation provide protection (thermal tolerance) to a subsequent exposure at 45/sup 0/C. During 40/sup 0/C HS, some HSPs become localized and stably associated with purified organelle fractions while others do not. A chase at 28/sup 0/C results in the gradual loss over a 4-hour period of the HSPs from the organelle fractions, but the HSPs remain selectively localized during a 40/sup 0/C chase period. The relative amount of HSPs which relocalize during a second HS increases with higher temperatures from 40/sup 0/C to 45/sup 0/C. Proteins induced by arsenite treatment are not selectively localized with organelle fractions at 28/sup 0/C but become organelle-associated during a subsequent HS at 40/sup 0/C.

  19. The Secreted Proteins of Achlya hypogyna and Thraustotheca clavata Identify the Ancestral Oomycete Secretome and Reveal Gene Acquisitions by Horizontal Gene Transfer

    PubMed Central

    Misner, Ian; Blouin, Nic; Leonard, Guy; Richards, Thomas A.; Lane, Christopher E.

    2015-01-01

    Saprotrophic and parasitic microorganisms secrete proteins into the environment to breakdown macromolecules and obtain nutrients. The molecules secreted are collectively termed the “secretome” and the composition and function of this set of proteins varies depending on the ecology, life cycle, and environment of an organism. Beyond the function of nutrient acquisition, parasitic lineages must also secrete molecules to manipulate their host. Here, we use a combination of de novo genome and transcriptome sequencing and bioinformatic identification of signal peptides to identify the putative secreted proteome of two oomycetes, the facultative parasite Achlya hypogyna and free-living Thraustotheca clavata. By comparing the secretomes of these saprolegnialean oomycetes with that of eight other oomycetes, we were able to characterize the evolution of this protein set across the oomycete clade. These species span the last common ancestor of the two major oomycete families allowing us to identify the ancestral secretome. This putative ancestral secretome consists of at least 84 gene families. Only 11 of these gene families are conserved across all 10 secretomes analyzed and the two major branches in the oomycete radiation. Notably, we have identified expressed elicitin-like effector genes in the saprotrophic decomposer, T. clavata. Phylogenetic analyses show six novel horizontal gene transfers to the oomycete secretome from bacterial and fungal donor lineages, four of which are specific to the Saprolegnialeans. Comparisons between free-living and pathogenic taxa highlight the functional changes of oomycete secretomes associated with shifts in lifestyle. PMID:25527045

  20. The Aspergillus fumigatus StuA Protein Governs the Up-Regulation of a Discrete Transcriptional Program during the Acquisition of Developmental CompetenceD⃞

    PubMed Central

    Sheppard, Donald C.; Doedt, Thomas; Chiang, Lisa Y.; Kim, H. Stanley; Chen, Dan; Nierman, William C.; Filler, Scott G.

    2005-01-01

    Members of the Asm1p, Phd1p, Sok2p, Efg1p, and StuAp (APSES) family of fungal proteins regulate morphogenesis and virulence in ascomycetes. We cloned the Aspergillus fumigatus APSES gene encoding StuAp and demonstrated that stuA transcription is markedly up-regulated after the acquisition of developmental competence. A. fumigatus ΔstuA mutants were impaired in their ability to undergo asexual reproduction. Conidiophore morphology was markedly abnormal, and only small numbers of dysmorphic conidia were produced, which exhibited precocious germination. Whole genome transcriptional analysis during the onset of developmental competence was performed and identified a subset of developmentally regulated genes that were stuA dependent, including a cluster of putative secondary metabolite biosynthesis genes, genes encoding proteins implicated in the regulation of morphogenesis, and genes encoding allergens and other antigenic proteins. Additionally, hyphae of the ΔstuA mutant displayed reduced expression of the catalase gene CAT1 and were hypersusceptible to hydrogen peroxide. PMID:16207816

  1. PsbU, a Protein Associated with Photosystem II, Is Required for the Acquisition of Cellular Thermotolerance in Synechococcus species PCC 70021

    PubMed Central

    Nishiyama, Yoshitaka; Los, Dmitry A.; Murata, Norio

    1999-01-01

    PsbU is an extrinsic protein of the photosystem II complex of cyanobacteria and red algae. Our previous in vitro studies (Y. Nishiyama, D.A. Los, H. Hayashi, N. Murata [1997] Plant Physiol 115: 1473–1480) revealed that PsbU stabilizes the oxygen-evolving machinery of the photosystem II complex against heat-induced inactivation in the cyanobacterium Synechococcus sp. PCC 7002. To elucidate the role of PsbU in vivo, we inactivated the psbU gene in Synechococcus sp. PCC 7002 by targeted mutagenesis. Inactivation of the psbU gene resulted in marked changes in the acclimative responses of cells to high temperature: Mutated cells were unable to increase the thermal stability of their oxygen-evolving machinery when grown at moderately high temperatures. Moreover, the cellular thermotolerance of the mutated cells failed to increase upon acclimation of cells to high temperature. The heat-shock response, as assessed in terms of the levels of homologs of the heat-shock proteins Hsp60, Hsp70, and Hsp17, was unaffected by the mutation in psbU, suggesting that heat-shock proteins were not involved in the changes in the acclimative responses. Our observations indicate that PsbU is involved in the mechanism that underlies the enhancement of the thermal stability of the oxygen-evolving machinery and that the stabilization of the oxygen-evolving machinery is crucial for the acquisition of cellular thermotolerance. PMID:10318707

  2. Penconazole induced changes in photosynthesis, ion acquisition and protein profile of Mentha pulegium L. under drought stress.

    PubMed

    Hassanpour, Halimeh; Khavari-Nejad, Ramazan Ali; Niknam, Vahid; Najafi, Farzaneh; Razavi, Khadijeh

    2013-10-01

    Effect of penconazole (PEN) treatment on drought-stressed Mentha pulegium L. plants was investigated. Six weeks after sowing, seedlings were grown under soil moisture corresponding to 100, 75, 50 and 25 % field capacity (FC) with or without PEN (15 mg l(-1)) for 4 weeks. Results showed that the seedlings at 75 % FC showed maximum growth and water supply lower than 75 % FC was the threshold of drought-initiated negative effects on seedling growth. Drought stress significantly induced proline and carbohydrate contents and the decreased chlorophyll, photosynthesis parameters, soluble proteins and ion accumulations. Exogenous PEN increased the growth parameters, pigments, photosynthesis and ion accumulations in drought stressed and unstressed plants, but the effects of PEN were more significant under water deficit conditions. PEN also reduced the negative effects of drought by osmotic balance and protein accumulations. Electrophoretic patterns indicated that PEN treatment increased the intensity of some protein bands with the molecular weights of 30 kDa in shoot and 31 kDa in roots, and several new protein bands with the molecular masses between 116 and 14 kDa appeared in leaves, shoots and roots. These results suggest that the PEN application can be a useful tool in alleviation of effects of drought stress in M. pulegium plants. PMID:24431517

  3. Analysis of protein biomarkers in human clinical tumor samples: critical aspects to success from tissue acquisition to analysis.

    PubMed

    Warren, Madhuri V; Chan, W Y Iris; Ridley, John M

    2011-04-01

    There has been increased interest in the analysis of protein biomarkers in clinical tumor tissues in recent years. Tissue-based biomarker assays can add value and aid decision-making at all stages of drug development, as well as being developed for use as predictive biomarkers and for patient stratification and prognostication in the clinic. However, there must be an awareness of the legal and ethical issues related to the sourcing of human tissue samples. This article also discusses the limits of scope and critical aspects on the successful use of the following tissue-based methods: immunohistochemistry, tissue microarrays and automated image analysis. Future advances in standardization of tissue biobanking methods, immunohistochemistry and quantitative image analysis techniques are also discussed. PMID:21473728

  4. Acquisition strategies

    SciTech Connect

    Zimmer, M.J.; Lynch, P.W. )

    1993-11-01

    Acquiring projects takes careful planning, research and consideration. Picking the right opportunities and avoiding the pitfalls will lead to a more valuable portfolio. This article describes the steps to take in evaluating an acquisition and what items need to be considered in an evaluation.

  5. Analysis of iron acquisition and storage-related genes in clinical and non-clinical strains of Yersinia enterocolitica biovar 1A.

    PubMed

    Kanaujia, Pawan Kumar; Bajaj, Priyanka; Virdi, Jugsharan Singh

    2015-10-01

    Possession of mechanisms for iron acquisition and its storage enhances the ability of the bacteria to survive in the iron-limiting environment of the host. In this study, 81 strains of Yersinia enterocolitica biovar 1A isolated from various clinical (n = 51) and non-clinical (n = 30) sources were investigated for the presence of the genes related to iron acquisition and storage. Important genes which were present in more than 85% of the strains included hasA, foxA, bfr, bfd, ftnA, and hmsT as well as the fhuCDB, fepBDGCfesfepA, feoAB, yfuABCD, hemPRSTUV, and hmsHFRS gene clusters. Majority of these genes is being reported for the first time in biovar 1A strains and showed significant homology with genes present in the known pathogenic biovars of Y. enterocolitica. However, no significant difference was observed in the distribution of iron acquisition and storage-related genes among clinical and non-clinical biovar 1A strains. Thus, it may be suggested that the presence of iron acquisition and storage-related genes per se might not be responsible for the supposedly better ability of clinical biovar 1A strains to cause infections in humans. However, in the backdrop of this data, the need to undertake functional studies are highly recommended. PMID:26223204

  6. Acquisition of “Start” and “Stop” response thresholds in peak-interval timing is differentially sensitive to protein synthesis inhibition in the dorsal and ventral striatum

    PubMed Central

    MacDonald, Christopher J.; Cheng, Ruey-Kuang; Meck, Warren H.

    2011-01-01

    Time-based decision-making in peak-interval timing procedures involves the setting of response thresholds for the initiation (“Start”) and termination (“Stop”) of a response sequence that is centered on a target duration. Using intracerebral infusions of the protein synthesis inhibitor anisomycin, we report that the acquisition of the “Start” response depends on normal functioning (including protein synthesis) in the dorsal striatum (DS), but not the ventral striatum (VS). Conversely, disruption of the VS, but not the DS, impairs the acquisition of the “Stop” response. We hypothesize that the dorsal and ventral regions of the striatum function as a competitive neural network that encodes the temporal boundaries marking the beginning and end of a timed response sequence. PMID:22435054

  7. Soy Protein Isolate Inhibits High-Fat Diet-Induced Senescence Pathways in Osteoblasts to Maintain Bone Acquisition in Male Rats

    PubMed Central

    Lazarenko, Oxana P.; Blackburn, Michael L.; Badger, Thomas M.; Ronis, Martin J. J.

    2015-01-01

    Chronic consumption by experimental animals of a typical Western diet high in saturated fats and cholesterol during postnatal life has been demonstrated to impair skeletal development. However, the underlying mechanism by which high-fat, energy-dense diets affect bone-forming cell phenotypes is poorly understood. Here, we show that male weanling rats fed a diet containing 45% fat and 0.5% cholesterol made with casein (HF-Cas) for 6 weeks displayed lower bone mineral density and strength compared with those of AIN-93G–fed dietary controls. Substitution of casein with soy protein isolate (SPI) in the high-fat diet (HF-SPI) prevented these effects. The bone-sparing effects of SPI were associated with prevention of HF-Cas–induced osteoblast senescence pathways through suppression of the p53/p21 signaling pathways. HF-Cas–fed rats had increased caveolin-1 and down-regulated Sirt1, leading to activations of peroxisome proliferator–activated receptor γ (PPARγ) and p53/p21, whereas rats fed HF-SPI suppressed caveolin-1 and activated Sirt1 to deacetylate PPARγ and p53 in bone. Treatment of osteoblastic cells with nonesterified free fatty acid (NEFA) increased cell senescence signaling pathways. Isoflavones significantly blocked activations of senescence-associated β-galactosidase and PPARγ/p53/p21 by NEFA. Finally, replicative senescent osteoblastic cells and bone marrow mesenchymal ST2 cells exhibited behavior similar to that of cells treated with NEFA and in vivo bone cells in rats fed the HF-Cas diet. These results suggest that (1) high concentrations of NEFA occurring with HF intake are mediators of osteoblast cell senescence leading to impairment of bone development and acquisition and (2) the molecular mechanisms underlying the SPI-protective effects involve isoflavone-induced inhibition of osteoblastic cell senescence to prevent HF-induced bone impairments. PMID:25490147

  8. Multidimensional Separation Using HILIC and SCX Pre-fractionation for RP LC-MS/MS Platform with Automated Exclusion List-based MS Data Acquisition with Increased Protein Quantification

    PubMed Central

    Zhou, Yu; Meng, Zhen; Edman-Woolcott, Maria; Hamm-Alvarez, Sarah F; Zandi, Ebrahim

    2015-01-01

    Liquid chromatography–mass spectrometry (LC-MS) based proteomics is one of the most widely used analytical platforms for global protein discovery and quantification. One of the challenges is the difficulty of identifying low abundance biomarker proteins from limited biological samples. Extensive fractionation could expand proteomics dynamic range, however, at the cost of high sample and time consumption. Extensive fractionation would increase the sample need and the labeling cost. Also quantitative proteomics depending on high resolution MS have the limitation of spectral acquisition speed. Those practical problems hinder the in-depth quantitative proteomics analysis such as tandem mass tag (TMT) experiments. We found the joint use of hydrophilic interaction liquid chromatography (HILIC) and strong cation exchange Chromatography (SCX) prefractionation at medium level could improve MS/MS efficiency, increase proteome coverage, shorten analysis time and save valuable samples. In addition, we scripted a program, Exclusion List Convertor (ELC), which automates and streamlines data acquisition workflow using the precursor ion exclusion (PIE) method. PIE reduces redundancy of high abundance MS/MS analyses by running replicates of the sample. The precursor ions detected in the initial run(s) are excluded for MS/MS in the subsequent run. We compared PIE methods with standard data dependent acquisition (DDA) methods running replicates without PIE for their effectiveness in quantifying TMT-tagged peptides and proteins in mouse tears. We quantified a total of 845 proteins and 1401 peptides using the PIE workflow, while the DDA method only resulted in 347 proteins and 731 peptides. This represents a 144% increase of protein identifications as a result of PIE analysis. PMID:26807013

  9. Late Mitochondrial Acquisition, Really?

    PubMed Central

    Degli Esposti, Mauro

    2016-01-01

    This article provides a timely critique of a recent Nature paper by Pittis and Gabaldón that has suggested a late origin of mitochondria in eukaryote evolution. It shows that the inferred ancestry of many mitochondrial proteins has been incorrectly assigned by Pittis and Gabaldón to bacteria other than the aerobic proteobacteria from which the ancestor of mitochondria originates, thereby questioning the validity of their suggestion that mitochondrial acquisition may be a late event in eukaryote evolution. The analysis and approach presented here may guide future studies to resolve the true ancestry of mitochondria. PMID:27289097

  10. A suite of pulse sequences based on multiple sequential acquisitions at one and two radiofrequency channels for solid-state magic-angle spinning NMR studies of proteins.

    PubMed

    Sharma, Kshama; Madhu, Perunthiruthy K; Mote, Kaustubh R

    2016-08-01

    One of the fundamental challenges in the application of solid-state NMR is its limited sensitivity, yet a majority of experiments do not make efficient use of the limited polarization available. The loss in polarization in a single acquisition experiment is mandated by the need to select out a single coherence pathway. In contrast, sequential acquisition strategies can encode more than one pathway in the same experiment or recover unused polarization to supplement a standard experiment. In this article, we present pulse sequences that implement sequential acquisition strategies on one and two radiofrequency channels with a combination of proton and carbon detection to record multiple experiments under magic-angle spinning. We show that complementary 2D experiments such as [Formula: see text] and [Formula: see text] or DARR and [Formula: see text], and 3D experiments such as [Formula: see text] and [Formula: see text], or [Formula: see text] and [Formula: see text]  can be combined in a single experiment to ensure time savings of at least 40 %. These experiments can be done under fast or slow-moderate magic-angle spinning frequencies aided by windowed [Formula: see text] acquisition and homonulcear decoupling. The pulse sequence suite is further expanded by including pathways that allow the recovery of residual polarization, the so-called 'afterglow' pathways, to encode a number of pulse sequences to aid in assignments and chemical-shift mapping. PMID:27364976

  11. Syntax acquisition.

    PubMed

    Crain, Stephen; Thornton, Rosalind

    2012-03-01

    Every normal child acquires a language in just a few years. By 3- or 4-years-old, children have effectively become adults in their abilities to produce and understand endlessly many sentences in a variety of conversational contexts. There are two alternative accounts of the course of children's language development. These different perspectives can be traced back to the nature versus nurture debate about how knowledge is acquired in any cognitive domain. One perspective dates back to Plato's dialog 'The Meno'. In this dialog, the protagonist, Socrates, demonstrates to Meno, an aristocrat in Ancient Greece, that a young slave knows more about geometry than he could have learned from experience. By extension, Plato's Problem refers to any gap between experience and knowledge. How children fill in the gap in the case of language continues to be the subject of much controversy in cognitive science. Any model of language acquisition must address three factors, inter alia: 1. The knowledge children accrue; 2. The input children receive (often called the primary linguistic data); 3. The nonlinguistic capacities of children to form and test generalizations based on the input. According to the famous linguist Noam Chomsky, the main task of linguistics is to explain how children bridge the gap-Chomsky calls it a 'chasm'-between what they come to know about language, and what they could have learned from experience, even given optimistic assumptions about their cognitive abilities. Proponents of the alternative 'nurture' approach accuse nativists like Chomsky of overestimating the complexity of what children learn, underestimating the data children have to work with, and manifesting undue pessimism about children's abilities to extract information based on the input. The modern 'nurture' approach is often referred to as the usage-based account. We discuss the usage-based account first, and then the nativist account. After that, we report and discuss the findings of several

  12. Investigating Second Language Acquisition.

    ERIC Educational Resources Information Center

    Jordens, Peter, Ed.; Lalleman, Josine, Ed.

    Essays in second language acquisition include: "The State of the Art in Second Language Acquisition Research" (Josine Lalleman); "Crosslinguistic Influence with Special Reference to the Acquisition of Grammar" (Michael Sharwood Smith); "Second Language Acquisition by Adult Immigrants: A Multiple Case Study of Turkish and Moroccan Learners of…

  13. Language Acquisition without an Acquisition Device

    ERIC Educational Resources Information Center

    O'Grady, William

    2012-01-01

    Most explanatory work on first and second language learning assumes the primacy of the acquisition phenomenon itself, and a good deal of work has been devoted to the search for an "acquisition device" that is specific to humans, and perhaps even to language. I will consider the possibility that this strategy is misguided and that language…

  14. Role of the Carboxy Terminus of SecA in Iron Acquisition, Protein Translocation, and Virulence of the Bacterial Pathogen Acinetobacter baumannii

    PubMed Central

    Fiester, Steven E.; Nwugo, Chika C.; Penwell, William F.; Neary, John M.; Beckett, Amber C.; Arivett, Brock A.; Schmidt, Robert E.; Geiger, Sarah C.; Connerly, Pamela L.; Menke, Sharon M.; Tomaras, Andrew P.

    2015-01-01

    Acinetobacter baumannii is a Gram-negative opportunistic nosocomial pathogen that causes pneumonia and soft tissue and systemic infections. Screening of a transposon insertion library of A. baumannii ATCC 19606T resulted in the identification of the 2010 derivative, which, although capable of growing well in iron-rich media, failed to prosper under iron chelation. Genetic, molecular, and functional assays showed that 2010's iron utilization-deficient phenotype is due to an insertion within the 3′ end of secA, which results in the production of a C-terminally truncated derivative of SecA. SecA plays a critical role in protein translocation through the SecYEG membrane channel. Accordingly, the secA mutation resulted in undetectable amounts of the ferric acinetobactin outer membrane receptor protein BauA while not affecting the production of other acinetobactin membrane protein transport components, such as BauB and BauE, or the secretion of acinetobactin by 2010 cells cultured in the presence of subinhibitory concentrations of the synthetic iron chelator 2,2′-dipyridyl. Outer membrane proteins involved in nutrient transport, adherence, and biofilm formation were also reduced in 2010. The SecA truncation also increased production of 30 different proteins, including proteins involved in adaptation/tolerance responses. Although some of these protein changes could negatively affect the pathobiology of the 2010 derivative, its virulence defect is mainly due to its inability to acquire iron via the acinetobactin-mediated system. These results together indicate that although the C terminus of the A. baumannii ATCC 19606T SecA is not essential for viability, it plays a critical role in the production and translocation of different proteins and virulence. PMID:25605767

  15. Acquisition of double-stranded DNA-binding ability in a hybrid protein between Escherichia coli CspA and the cold shock domain of human YB-1.

    PubMed

    Wang, N; Yamanaka, K; Inouye, M

    2000-11-01

    Escherichia coli CspA, a major cold shock protein, is dramatically induced upon temperature downshift. As it binds co-operatively to single-stranded DNA (ssDNA) and RNA without apparent sequence specificity, it has been proposed that CspA acts as an RNA chaperone to facilitate transcription and translation at low temperature. CspA consists of a five-stranded beta-barrel structure containing two RNA-binding motifs, RNP1 and RNP2. Eukaryotic Y-box proteins, such as human YB-1, are a family of nucleic acid-binding proteins that share a region of high homology with CspA (43% identity), termed the cold shock domain (CSD). Their cellular functions are very diverse and are associated with growth-related processes. Here, we replaced the six-residue loop region of CspA between the beta3 and beta4 strands with the corresponding region of the CSD of human YB-1 protein. The resulting hybrid protein became capable of binding to double-stranded DNA (dsDNA) in addition to ssDNA and RNA. The dsDNA-binding ability of an RNP1 point mutant (F20L) of the hybrid was almost unchanged. On the other hand, the dsDNA-binding ability of the hybrid protein was abolished in high salt concentrations in contrast to its ssDNA-binding ability. These results indicate that the loop region between the beta3 and beta4 strands of Y-box proteins, which is a little longer and more basic than that of CspA, plays an important role in their binding to dsDNA. PMID:11069676

  16. EARLY SYNTACTIC ACQUISITION.

    ERIC Educational Resources Information Center

    KELLEY, K.L.

    THIS PAPER IS A STUDY OF A CHILD'S EARLIEST PRETRANSFORMATIONAL LANGUAGE ACQUISITION PROCESSES. A MODEL IS CONSTRUCTED BASED ON THE ASSUMPTIONS (1) THAT SYNTACTIC ACQUISITION OCCURS THROUGH THE TESTING OF HYPOTHESES REFLECTING THE INITIAL STRUCTURE OF THE ACQUISITION MECHANISM AND THE LANGUAGE DATA TO WHICH THE CHILD IS EXPOSED, AND (2) THAT…

  17. Bicarbonate uptake via an anion exchange protein is the main mechanism of inorganic carbon acquisition by the giant kelp Macrocystis pyrifera (Laminariales, Phaeophyceae) under variable pH.

    PubMed

    Fernández, Pamela A; Hurd, Catriona L; Roleda, Michael Y

    2014-12-01

    Macrocystis pyrifera is a widely distributed, highly productive, seaweed. It is known to use bicarbonate (HCO3 (-) ) from seawater in photosynthesis and the main mechanism of utilization is attributed to the external catalyzed dehydration of HCO3 (-) by the surface-bound enzyme carbonic anhydrase (CAext ). Here, we examined other putative HCO3 (-) uptake mechanisms in M. pyrifera under pHT 9.00 (HCO3 (-) : CO2  = 940:1) and pHT 7.65 (HCO3 (-) : CO2  = 51:1). Rates of photosynthesis, and internal CA (CAint ) and CAext activity were measured following the application of AZ which inhibits CAext , and DIDS which inhibits a different HCO3 (-) uptake system, via an anion exchange (AE) protein. We found that the main mechanism of HCO3 (-) uptake by M. pyrifera is via an AE protein, regardless of the HCO3 (-) : CO2 ratio, with CAext making little contribution. Inhibiting the AE protein led to a 55%-65% decrease in photosynthetic rates. Inhibiting both the AE protein and CAext at pHT 9.00 led to 80%-100% inhibition of photosynthesis, whereas at pHT 7.65, passive CO2 diffusion supported 33% of photosynthesis. CAint was active at pHT 7.65 and 9.00, and activity was always higher than CAext , because of its role in dehydrating HCO3 (-) to supply CO2 to RuBisCO. Interestingly, the main mechanism of HCO3 (-) uptake in M. pyrifera was different than that in other Laminariales studied (CAext -catalyzed reaction) and we suggest that species-specific knowledge of carbon uptake mechanisms is required in order to elucidate how seaweeds might respond to future changes in HCO3 (-) :CO2 due to ocean acidification. PMID:26988782

  18. Arf1-GTP-induced Tubule Formation Suggests a Function of Arf Family Proteins in Curvature Acquisition at Sites of Vesicle Budding*

    PubMed Central

    Krauss, Michael; Jia, Jun-Yong; Roux, Aurélien; Beck, Rainer; Wieland, Felix T.; De Camilli, Pietro; Haucke, Volker

    2008-01-01

    ADP-ribosylation factor (Arf) and related small GTPases play crucial roles in membrane traffic within the exo- and endocytic pathways. Arf proteins in their GTP-bound state are associated with curved membrane buds and tubules, frequently together with effector coat proteins to which they bind. Here we report that Arf1 is found on membrane tubules originating from the Golgi complex where it colocalizes with COPI and GGA1 vesicle coat proteins. Arf1 also induces tubulation of liposomes in vitro. Mutations within the amino-terminal amphipathic helix (NTH) of Arf1 affect the number of Arf1-positive tubules in vivo and its property to tubulate liposomes. Moreover, hydrophilic substitutions within the hydrophobic part of its NTH impair Arf1-catalyzed budding of COPI vesicles in vitro. Our data indicate that GTP-controlled local induction of high curvature membranes is an important property of Arf1 that might be shared by a subgroup of Arf/Arl family GTPases. PMID:18693248

  19. Protein

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Proteins are the major structural and functional components of all cells in the body. They are macromolecules that comprise 1 or more chains of amino acids that vary in their sequence and length and are folded into specific 3-dimensional structures. The sizes and conformations of proteins, therefor...

  20. Proteins.

    ERIC Educational Resources Information Center

    Doolittle, Russell F.

    1985-01-01

    Examines proteins which give rise to structure and, by virtue of selective binding to other molecules, make genes. Binding sites, amino acids, protein evolution, and molecular paleontology are discussed. Work with encoding segments of deoxyribonucleic acid (exons) and noncoding stretches (introns) provides new information for hypotheses. (DH)

  1. Duffy Antigen Receptor for Chemokine (DARC) Polymorphisms and Its Involvement in Acquisition of Inhibitory Anti-Duffy Binding Protein II (DBPII) Immunity

    PubMed Central

    Santos-Alves, Jessica R.; Tang, Michaelis Loren; Sanchez, Bruno A. M.; Sousa, Tais N.; Fontes, Cor J. F.; Nogueira, Paulo A.; Rocha, Roberto S.; Brito, Cristiana F. A.; Adams, John H.; Kano, Flora S.; Carvalho, Luzia H.

    2014-01-01

    The Plasmodium vivax Duffy binding protein (PvDBP) and its erythrocytic receptor, the Duffy antigen receptor for chemokines (DARC), are involved in the major P. vivax erythrocyte invasion pathway. An open cohort study to analyze DARC genotypes and their relationship to PvDBP immune responses was carried out in 620 volunteers in an agricultural settlement of the Brazilian Amazon. Three cross-sectional surveys were conducted at 6-month intervals, comprising 395, 410, and 407 subjects, respectively. The incidence rates of P. vivax infection was 2.32 malaria episodes per 100 person-months under survey (95% confidence interval [CI] of 1.92-2.80/100 person-month) and, of P. falciparum, 0.04 per 100 person-months (95% CI of 0.007–0.14/100 person-month). The distribution of DARC genotypes was consistent with the heterogeneous ethnic origins of the Amazon population, with a predominance of non-silent DARC alleles: FY*A > FY*B. The 12-month follow-up study demonstrated no association between DARC genotypes and total IgG antibodies as measured by ELISA targeting PvDBP (region II, DBPII or regions II–IV, DBPII-IV). The naturally acquired DBPII specific binding inhibitory antibodies (BIAbs) tended to be more frequent in heterozygous individuals carrying a DARC-silent allele (FY*BES). These results provide evidence that DARC polymorphisms may influence the naturally acquired inhibitory anti-Duffy binding protein II immunity. PMID:24710306

  2. Excessive acquisition in hoarding.

    PubMed

    Frost, Randy O; Tolin, David F; Steketee, Gail; Fitch, Kristin E; Selbo-Bruns, Alexandra

    2009-06-01

    Compulsive hoarding (the acquisition of and failure to discard large numbers of possessions) is associated with substantial health risk, impairment, and economic burden. However, little research has examined separate components of this definition, particularly excessive acquisition. The present study examined acquisition in hoarding. Participants, 878 self-identified with hoarding and 665 family informants (not matched to hoarding participants), completed an Internet survey. Among hoarding participants who met criteria for clinically significant hoarding, 61% met criteria for a diagnosis of compulsive buying and approximately 85% reported excessive acquisition. Family informants indicated that nearly 95% exhibited excessive acquisition. Those who acquired excessively had more severe hoarding; their hoarding had an earlier onset and resulted in more psychiatric work impairment days; and they experienced more symptoms of obsessive-compulsive disorder, depression, and anxiety. Two forms of excessive acquisition (buying and free things) each contributed independent variance in the prediction of hoarding severity and related symptoms. PMID:19261435

  3. Excessive Acquisition in Hoarding

    PubMed Central

    Frost, Randy O.; Tolin, David F.; Steketee, Gail; Fitch, Kristin E.; Selbo-Bruns, Alexandra

    2009-01-01

    Compulsive hoarding (the acquisition of and failure to discard large numbers of possessions) is associated with substantial health risk, impairment, and economic burden. However, little research has examined separate components of this definition, particularly excessive acquisition. The present study examined acquisition in hoarding. Participants, 878 self-identified with hoarding and 665 family informants (not matched to hoarding participants), completed an internet survey. Among hoarding participants who met criteria for clinically significant hoarding, 61% met criteria for a diagnosis of compulsive buying and approximately 85% reported excessive acquisition. Family informants indicated that nearly 95% exhibited excessive acquisition. Those who acquired excessively had more severe hoarding; their hoarding had an earlier onset and resulted in more psychiatric work impairment days; and they experienced more symptoms of obsessive-compulsive disorder, depression, and anxiety. Two forms of excessive acquisition (buying and free things) each contributed independent variance in the prediction of hoarding severity and related symptoms. PMID:19261435

  4. Replicating Adenovirus-Simian Immunodeficiency Virus (SIV) Recombinant Priming and Envelope Protein Boosting Elicits Localized, Mucosal IgA Immunity in Rhesus Macaques Correlated with Delayed Acquisition following a Repeated Low-Dose Rectal SIVmac251 Challenge

    PubMed Central

    Xiao, Peng; Patterson, L. Jean; Kuate, Seraphin; Brocca-Cofano, Egidio; Thomas, Michael A.; Venzon, David; Zhao, Jun; DiPasquale, Janet; Fenizia, Claudio; Lee, Eun Mi; Kalisz, Irene; Kalyanaraman, Vaniambadi S.; Pal, Ranajit; Montefiori, David; Keele, Brandon F.

    2012-01-01

    We have shown that sequential replicating adenovirus type 5 host range mutant human immunodeficiency virus/simian immunodeficiency virus (HIV/SIV) recombinant priming delivered first intranasally (i.n.) plus orally and then intratracheally (i.t.), followed by envelope protein boosting, elicits broad cellular immunity and functional, envelope-specific serum and mucosal antibodies that correlate with protection from high-dose SIV and simian/human immunodeficiency virus (SHIV) challenges in rhesus macaques. Here we extended these studies to compare the standard i.n./i.t. regimen with additional mucosal administration routes, including sublingual, rectal, and vaginal routes. Similar systemic cellular and humoral immunity was elicited by all immunization routes. Central and effector memory T cell responses were also elicited by the four immunization routes in bronchoalveolar lavage fluid and jejunal, rectal, and vaginal tissue samples. Cellular responses in vaginal tissue were more compartmentalized, being induced primarily by intravaginal administration. In contrast, all immunization routes elicited secretory IgA (sIgA) responses at multiple mucosal sites. Following a repeated low-dose intrarectal (i.r.) challenge with SIVmac251 at a dose transmitting one or two variants, protection against acquisition was not achieved except in one macaque in the i.r. immunized group. All immunized macaques exhibited reduced peak viremia compared to that of controls, correlated inversely with prechallenge serum antienvelope avidity, antibody-dependent cellular cytotoxicity (ADCC) titers, and percent antibody-dependent cell-mediated viral inhibition. Both antibody avidity and ADCC titers were correlated with the number of exposures required for infection. Notably, we show for the first time a significant correlation of vaccine-induced sIgA titers in rectal secretions with delayed acquisition. Further investigation of the characteristics and properties of the sIgA should elucidate the

  5. Streamlined acquisition handbook

    NASA Technical Reports Server (NTRS)

    1990-01-01

    NASA has always placed great emphasis on the acquisition process, recognizing it as among its most important activities. This handbook is intended to facilitate the application of streamlined acquisition procedures. The development of these procedures reflects the efforts of an action group composed of NASA Headquarters and center acquisition professionals. It is the intent to accomplish the real change in the acquisition process as a result of this effort. An important part of streamlining the acquisition process is a commitment by the people involved in the process to accomplishing acquisition activities quickly and with high quality. Too often we continue to accomplish work in 'the same old way' without considering available alternatives which would require no changes to regulations, approvals from Headquarters, or waivers of required practice. Similarly, we must be sensitive to schedule opportunities throughout the acquisition cycle, not just once the purchase request arrives at the procurement office. Techniques that have been identified as ways of reducing acquisition lead time while maintaining high quality in our acquisition process are presented.

  6. Coring Sample Acquisition Tool

    NASA Technical Reports Server (NTRS)

    Haddad, Nicolas E.; Murray, Saben D.; Walkemeyer, Phillip E.; Badescu, Mircea; Sherrit, Stewart; Bao, Xiaoqi; Kriechbaum, Kristopher L.; Richardson, Megan; Klein, Kerry J.

    2012-01-01

    A sample acquisition tool (SAT) has been developed that can be used autonomously to sample drill and capture rock cores. The tool is designed to accommodate core transfer using a sample tube to the IMSAH (integrated Mars sample acquisition and handling) SHEC (sample handling, encapsulation, and containerization) without ever touching the pristine core sample in the transfer process.

  7. Acquisition of teleological descriptions

    NASA Astrophysics Data System (ADS)

    Franke, David W.

    1992-03-01

    Teleology descriptions capture the purpose of an entity, mechanism, or activity with which they are associated. These descriptions can be used in explanation, diagnosis, and design reuse. We describe a technique for acquiring teleological descriptions expressed in the teleology language TeD. Acquisition occurs during design by observing design modifications and design verification. We demonstrate the acquisition technique in an electronic circuit design.

  8. Human target acquisition performance

    NASA Astrophysics Data System (ADS)

    Teaney, Brian P.; Du Bosq, Todd W.; Reynolds, Joseph P.; Thompson, Roger; Aghera, Sameer; Moyer, Steven K.; Flug, Eric; Espinola, Richard; Hixson, Jonathan

    2012-06-01

    The battlefield has shifted from armored vehicles to armed insurgents. Target acquisition (identification, recognition, and detection) range performance involving humans as targets is vital for modern warfare. The acquisition and neutralization of armed insurgents while at the same time minimizing fratricide and civilian casualties is a mounting concern. U.S. Army RDECOM CERDEC NVESD has conducted many experiments involving human targets for infrared and reflective band sensors. The target sets include human activities, hand-held objects, uniforms & armament, and other tactically relevant targets. This paper will define a set of standard task difficulty values for identification and recognition associated with human target acquisition performance.

  9. Interactive knowledge acquisition tools

    NASA Technical Reports Server (NTRS)

    Dudziak, Martin J.; Feinstein, Jerald L.

    1987-01-01

    The problems of designing practical tools to aid the knowledge engineer and general applications used in performing knowledge acquisition tasks are discussed. A particular approach was developed for the class of knowledge acquisition problem characterized by situations where acquisition and transformation of domain expertise are often bottlenecks in systems development. An explanation is given on how the tool and underlying software engineering principles can be extended to provide a flexible set of tools that allow the application specialist to build highly customized knowledge-based applications.

  10. Acquisition signal transmitter

    NASA Technical Reports Server (NTRS)

    Friedman, Morton L. (Inventor)

    1989-01-01

    An encoded information transmitter which transmits a radio frequency carrier that is amplitude modulated by a constant frequency waveform and thereafter amplitude modulated by a predetermined encoded waveform, the constant frequency waveform modulated carrier constituting an acquisition signal and the encoded waveform modulated carrier constituting an information bearing signal, the acquisition signal providing enhanced signal acquisition and interference rejection favoring the information bearing signal. One specific application for this transmitter is as a distress transmitter where a conventional, legislated audio tone modulated signal is transmitted followed first by the acquisition signal and then the information bearing signal, the information bearing signal being encoded with, among other things, vehicle identification data. The acquistion signal enables a receiver to acquire the information bearing signal where the received signal is low and/or where the received signal has a low signal-to-noise ratio in an environment where there are multiple signals in the same frequency band as the information bearing signal.

  11. High Speed data acquisition

    SciTech Connect

    Cooper, Peter S.

    1998-02-01

    A general introduction to high Speed data acquisition system techniques in modern particle physics experiments is given. Examples are drawn from the SELEX(E781) high statistics charmed baryon production and decay experiment now taking data at Fermilab.

  12. Documentation and knowledge acquisition

    NASA Technical Reports Server (NTRS)

    Rochowiak, Daniel; Moseley, Warren

    1990-01-01

    Traditional approaches to knowledge acquisition have focused on interviews. An alternative focuses on the documentation associated with a domain. Adopting a documentation approach provides some advantages during familiarization. A knowledge management tool was constructed to gain these advantages.

  13. Data acquisition system

    DOEpatents

    Shapiro, Stephen L.; Mani, Sudhindra; Atlas, Eugene L.; Cords, Dieter H. W.; Holbrook, Britt

    1997-01-01

    A data acquisition circuit for a particle detection system that allows for time tagging of particles detected by the system. The particle detection system screens out background noise and discriminate between hits from scattered and unscattered particles. The detection system can also be adapted to detect a wide variety of particle types. The detection system utilizes a particle detection pixel array, each pixel containing a back-biased PIN diode, and a data acquisition pixel array. Each pixel in the particle detection pixel array is in electrical contact with a pixel in the data acquisition pixel array. In response to a particle hit, the affected PIN diodes generate a current, which is detected by the corresponding data acquisition pixels. This current is integrated to produce a voltage across a capacitor, the voltage being related to the amount of energy deposited in the pixel by the particle. The current is also used to trigger a read of the pixel hit by the particle.

  14. Rx for Acquisitions Hangups

    ERIC Educational Resources Information Center

    Huleatt, Richard S.

    1973-01-01

    A system of ordering library materials efficiently, quickly and at low cost is presented. The procedure bypasses purchasing departments and helps reduce acquisitions time by authorizing direct ordering by the library. Forms and procedures used are discussed. (1 reference) (DH)

  15. FOS Target Acquisition Test

    NASA Astrophysics Data System (ADS)

    Koratkar, Anuradha

    1994-01-01

    FOS onboard target acquisition software capabilities will be verified by this test -- point source binary, point source firmware, point source peak-up, wfpc2 assisted realtime, point source peak-down, taled assisted binary, taled assisted firmware, and nth star binary modes. The primary modes are tested 3 times to determine repeatability. This test is the only test that will verify mode-to-mode acquisition offsets. This test has to be conducted for both the RED and BLUE detectors.

  16. Acquisition of Comparison Constructions

    ERIC Educational Resources Information Center

    Hohaus, Vera; Tiemann, Sonja; Beck, Sigrid

    2014-01-01

    This article presents a study on the time course of the acquisition of comparison constructions. The order in which comparison constructions (comparatives, measure phrases, superlatives, degree questions, etc.) show up in English- and German-learning children's spontaneous speech is quite fixed. It is shown to be insufficiently determined by…

  17. High Speed data acquisition

    SciTech Connect

    Cooper, P.S.

    1998-02-01

    A general introduction to high Speed data acquisition system techniques in modern particle physics experiments is given. Examples are drawn from the SELEX(E781) high statistics charmed baryon production and decay experiment now taking data at Fermilab. {copyright} {ital 1998 American Institute of Physics.}

  18. Image Acquisition Context

    PubMed Central

    Bidgood, W. Dean; Bray, Bruce; Brown, Nicolas; Mori, Angelo Rossi; Spackman, Kent A.; Golichowski, Alan; Jones, Robert H.; Korman, Louis; Dove, Brent; Hildebrand, Lloyd; Berg, Michael

    1999-01-01

    Objective: To support clinically relevant indexing of biomedical images and image-related information based on the attributes of image acquisition procedures and the judgments (observations) expressed by observers in the process of image interpretation. Design: The authors introduce the notion of “image acquisition context,” the set of attributes that describe image acquisition procedures, and present a standards-based strategy for utilizing the attributes of image acquisition context as indexing and retrieval keys for digital image libraries. Methods: The authors' indexing strategy is based on an interdependent message/terminology architecture that combines the Digital Imaging and Communication in Medicine (DICOM) standard, the SNOMED (Systematized Nomenclature of Human and Veterinary Medicine) vocabulary, and the SNOMED DICOM microglossary. The SNOMED DICOM microglossary provides context-dependent mapping of terminology to DICOM data elements. Results: The capability of embedding standard coded descriptors in DICOM image headers and image-interpretation reports improves the potential for selective retrieval of image-related information. This favorably affects information management in digital libraries. PMID:9925229

  19. Data Acquisition Backend

    SciTech Connect

    Britton Jr., Charles L.; Ezell, N. Dianne Bull; Roberts, Michael

    2013-10-01

    This document is intended to summarize the development and testing of the data acquisition module portion of the Johnson Noise Thermometry (JNT) system developed at ORNL. The proposed system has been presented in an earlier report [1]. A more extensive project background including the project rationale is available in the initial project report [2].

  20. [Acquisition of arithmetic knowledge].

    PubMed

    Fayol, Michel

    2008-01-01

    The focus of this paper is on contemporary research on the number counting and arithmetical competencies that emerge during infancy, the preschool years, and the elementary school. I provide a brief overview of the evolution of children's conceptual knowledge of arithmetic knowledge, the acquisition and use of counting and how they solve simple arithmetic problems (e.g. 4 + 3). PMID:18198117

  1. Acquisitions List No. 42.

    ERIC Educational Resources Information Center

    Planned Parenthood--World Population, New York, NY. Katherine Dexter McCormick Library.

    The "Acquisitions List" of demographic books and articles is issued every two months by the Katharine Dexter McCormick Library. Divided into two parts, the first contains a list of books most recently acquired by the Library, each one annotated and also marked with the Library call number. The second part consists of a list of annotated articles,…

  2. Acquisitions List No. 43.

    ERIC Educational Resources Information Center

    Planned Parenthood--World Population, New York, NY. Katherine Dexter McCormick Library.

    The "Acquisitions List" of demographic books and articles is issued every two months by the Katharine Dexter McCormick Library. Divided into two parts, the first contains a list of books most recently acquired by the Library, each one annotated and also marked with the Library call number. The second part consists of a list of annotated articles,…

  3. Following Native Language Acquisition.

    ERIC Educational Resources Information Center

    Neiburg, Michael S.

    Native language acquisition is a natural and non-natural stage-by-stage process. The natural first stage is development of speech and listening skills. In this stage, competency is gained in the home environment. The next, non-natural stage is development of literacy, a cultural skill taught in school. Since oral-aural native language development…

  4. Telecommunications and data acquisition

    NASA Technical Reports Server (NTRS)

    Renzetti, N. A. (Editor)

    1981-01-01

    Deep Space Network progress in flight project support, tracking and data acquisition research and technology, network engineering, hardware and software implementation, and operations is reported. In addition, developments in Earth based radio technology as applied to geodynamics, astrophysics, and the radio search for extraterrestrial intelligence are reported.

  5. Coordinating Council. Seventh Meeting: Acquisitions

    NASA Technical Reports Server (NTRS)

    1992-01-01

    The theme for this NASA Scientific and Technical Information Program Coordinating Council meeting was Acquisitions. In addition to NASA and the NASA Center for AeroSpace Information (CASI) presentations, the report contains fairly lengthy visuals about acquisitions at the Defense Technical Information Center. CASI's acquisitions program and CASI's proactive acquisitions activity were described. There was a presentation on the document evaluation process at CASI. A talk about open literature scope and coverage at the American Institute of Aeronautics and Astronautics was also given. An overview of the STI Program's Acquisitions Experts Committee was given next. Finally acquisitions initiatives of the NASA STI program were presented.

  6. 33. Perimeter acquisition radar building room #320, perimeter acquisition radar ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    33. Perimeter acquisition radar building room #320, perimeter acquisition radar operations center (PAROC), contains the tactical command and control group equipment required to control the par site. Showing spacetrack monitor console - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

  7. Semantic Approaches to Language Acquisition

    ERIC Educational Resources Information Center

    Stemmer, N.

    1973-01-01

    Critical evaluation of Schlesinger's theory of language acquisition as expounded in Production of Utterances and Language Acquisition'' in The Ontogenesis of Grammar'', p63-101, New York: Academic Press, 1971. (RS)

  8. Data acquisition instruments: Psychopharmacology

    SciTech Connect

    Hartley, D.S. III

    1998-01-01

    This report contains the results of a Direct Assistance Project performed by Lockheed Martin Energy Systems, Inc., for Dr. K. O. Jobson. The purpose of the project was to perform preliminary analysis of the data acquisition instruments used in the field of psychiatry, with the goal of identifying commonalities of data and strategies for handling and using the data in the most advantageous fashion. Data acquisition instruments from 12 sources were provided by Dr. Jobson. Several commonalities were identified and a potentially useful data strategy is reported here. Analysis of the information collected for utility in performing diagnoses is recommended. In addition, further work is recommended to refine the commonalities into a directly useful computer systems structure.

  9. Automatic carrier acquisition system

    NASA Technical Reports Server (NTRS)

    Bunce, R. C. (Inventor)

    1973-01-01

    An automatic carrier acquisition system for a phase locked loop (PLL) receiver is disclosed. It includes a local oscillator, which sweeps the receiver to tune across the carrier frequency uncertainty range until the carrier crosses the receiver IF reference. Such crossing is detected by an automatic acquisition detector. It receives the IF signal from the receiver as well as the IF reference. It includes a pair of multipliers which multiply the IF signal with the IF reference in phase and in quadrature. The outputs of the multipliers are filtered through bandpass filters and power detected. The output of the power detector has a signal dc component which is optimized with respect to the noise dc level by the selection of the time constants of the filters as a function of the sweep rate of the local oscillator.

  10. Advanced Data Acquisition Systems

    NASA Technical Reports Server (NTRS)

    Perotti, J.

    2003-01-01

    Current and future requirements of the aerospace sensors and transducers field make it necessary for the design and development of new data acquisition devices and instrumentation systems. New designs are sought to incorporate self-health, self-calibrating, self-repair capabilities, allowing greater measurement reliability and extended calibration cycles. With the addition of power management schemes, state-of-the-art data acquisition systems allow data to be processed and presented to the users with increased efficiency and accuracy. The design architecture presented in this paper displays an innovative approach to data acquisition systems. The design incorporates: electronic health self-check, device/system self-calibration, electronics and function self-repair, failure detection and prediction, and power management (reduced power consumption). These requirements are driven by the aerospace industry need to reduce operations and maintenance costs, to accelerate processing time and to provide reliable hardware with minimum costs. The project's design architecture incorporates some commercially available components identified during the market research investigation like: Field Programmable Gate Arrays (FPGA) Programmable Analog Integrated Circuits (PAC IC) and Field Programmable Analog Arrays (FPAA); Digital Signal Processing (DSP) electronic/system control and investigation of specific characteristics found in technologies like: Electronic Component Mean Time Between Failure (MTBF); and Radiation Hardened Component Availability. There are three main sections discussed in the design architecture presented in this document. They are the following: (a) Analog Signal Module Section, (b) Digital Signal/Control Module Section and (c) Power Management Module Section. These sections are discussed in detail in the following pages. This approach to data acquisition systems has resulted in the assignment of patent rights to Kennedy Space Center under U.S. patent # 6

  11. Data Acquisition Systems

    NASA Technical Reports Server (NTRS)

    1989-01-01

    Technology developed during a joint research program with Langley and Kinetic Systems Corporation led to Kinetic Systems' production of a high speed Computer Automated Measurement and Control (CAMAC) data acquisition system. The study, which involved the use of CAMAC equipment applied to flight simulation, significantly improved the company's technical capability and produced new applications. With Digital Equipment Corporation, Kinetic Systems is marketing the system to government and private companies for flight simulation, fusion research, turbine testing, steelmaking, etc.

  12. MDSplus data acquisition system

    SciTech Connect

    Stillerman, J.A.; Fredian, T.W.; Klare, K.; Manduchi, G.

    1997-01-01

    MDSplus, a tree based, distributed data acquisition system, was developed in collaboration with the ZTH Group at Los Alamos National Lab and the RFX Group at CNR in Padua, Italy. It is currently in use at MIT, RFX in Padua, TCV at EPFL in Lausanne, and KBSI in South Korea. MDSplus is made up of a set of X/motif based tools for data acquisition and display, as well as diagnostic configuration and management. It is based on a hierarchical experiment description which completely describes the data acquisition and analysis tasks and contains the results from these operations. These tools were designed to operate in a distributed, client/server environment with multiple concurrent readers and writers to the data store. While usually used over a Local Area Network, these tools can be used over the Internet to provide access for remote diagnosticians and even machine operators. An interface to a relational database is provided for storage and management of processed data. IDL is used as the primary data analysis and visualization tool. IDL is a registered trademark of Research Systems Inc. {copyright} {ital 1996 American Institute of Physics.}

  13. The Acquisition of Colour Terms.

    ERIC Educational Resources Information Center

    Andrich, Gail Rex; Tager-Flusberg, Helen

    1986-01-01

    Reports two studies which investigated the acquisition of color terms by preschool children. The first was designed to clarify the role of certain conceptual factors in the acquisition of color terms. The second explored how input may interact with these conceptual factors and help to guide the acquisition of color words. (SED)

  14. First Language Acquisition and Teaching

    ERIC Educational Resources Information Center

    Cruz-Ferreira, Madalena

    2011-01-01

    "First language acquisition" commonly means the acquisition of a single language in childhood, regardless of the number of languages in a child's natural environment. Language acquisition is variously viewed as predetermined, wondrous, a source of concern, and as developing through formal processes. "First language teaching" concerns schooling in…

  15. Data Acquisition Systems

    NASA Technical Reports Server (NTRS)

    1994-01-01

    In the mid-1980s, Kinetic Systems and Langley Research Center determined that high speed CAMAC (Computer Automated Measurement and Control) data acquisition systems could significantly improve Langley's ARTS (Advanced Real Time Simulation) system. The ARTS system supports flight simulation R&D, and the CAMAC equipment allowed 32 high performance simulators to be controlled by centrally located host computers. This technology broadened Kinetic Systems' capabilities and led to several commercial applications. One of them is General Atomics' fusion research program. Kinetic Systems equipment allows tokamak data to be acquired four to 15 times more rapidly. Ford Motor company uses the same technology to control and monitor transmission testing facilities.

  16. Early object rule acquisition.

    PubMed

    Pierce, D E

    1991-05-01

    The purpose of this study was to generate a grounded theory of early object rule acquisition. The grounded theory approach and computer coding were used to analyze videotaped samples of an infant's and a toddler's independent object play, which produced the categories descriptive of three primary types of object rules; rules of object properties, rules of object action, and rules of object affect. This occupational science theory offers potential for understanding the role of objects in human occupations, for development of instruments, and for applications in occupational therapy early intervention. PMID:2048625

  17. HYPERCP data acquisition system

    SciTech Connect

    Kaplan, D.M.; Luebke, W.R.; Chakravorty, A.

    1997-12-31

    For the HyperCP experiment at Fermilab, we have assembled a data acquisition system that records on up to 45 Exabyte 8505 tape drives in parallel at up to 17 MB/s. During the beam spill, data axe acquired from the front-end digitization systems at {approx} 60 MB/s via five parallel data paths. The front-end systems achieve typical readout deadtime of {approx} 1 {mu}s per event, allowing operation at 75-kHz trigger rate with {approx_lt}30% deadtime. Event building and tapewriting are handled by 15 Motorola MVME167 processors in 5 VME crates.

  18. Data-acquisition systems

    SciTech Connect

    Cyborski, D.R.; Teh, K.M.

    1995-08-01

    Up to now, DAPHNE, the data-acquisition system developed for ATLAS, was used routinely for experiments at ATLAS and the Dynamitron. More recently, the Division implemented 2 MSU/DAPHNE systems. The MSU/DAPHNE system is a hybrid data-acquisition system which combines the front-end of the Michigan State University (MSU) DA system with the traditional DAPHNE back-end. The MSU front-end is based on commercially available modules. This alleviates the problems encountered with the DAPHNE front-end which is based on custom designed electronics. The first MSU system was obtained for the APEX experiment and was used there successfully. A second MSU front-end, purchased as a backup for the APEX experiment, was installed as a fully-independent second MSU/DAPHNE system with the procurement of a DEC 3000 Alpha host computer, and was used successfully for data-taking in an experiment at ATLAS. Additional hardware for a third system was bought and will be installed. With the availability of 2 MSU/DAPHNE systems in addition to the existing APEX setup, it is planned that the existing DAPHNE front-end will be decommissioned.

  19. Portable data acquisition system

    SciTech Connect

    Bowers, J; Rogers, H

    1999-05-03

    Lawrence Livermore National Laboratory (LLNL) has developed a Portable Data Acquisition (DAQ) System that is basically a laboratory-scale of Program Logic Control (PLC). This DAQ system can obtain signals from numerous sensors (e.g., pH, level, pressure, flow meters), open and close valves, and turn on and off pumps. The data can then be saved on a spreadsheet or displayed as a graph/indicator in real-time on a computer screen. The whole DAQ system was designed to be portable so that it could sit on a bench top during laboratory-scale treatability studies, or moved out into the field during larger studies. This DAQ system is also fairly simple to use. All that is required is some working knowledge of LabVIEW 4.1, and how to properly wire the process equipment. The DAQ system has been used during treatability studies on cesium precipitation, controlled hydrolysis of water- reactive wastes, and other waste treatment studies that enable LLNL to comply with the Federal Facility Compliance Act (FFCAct). Improved data acquisition allows the study to be better monitored, and therefore better controlled, and can be used to determine the results of the treatment study more effectively. This also contributes to the design of larger treatment processes.

  20. 48 CFR 873.105 - Acquisition planning.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Acquisition planning. 873.105 Section 873.105 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS DEPARTMENT SUPPLEMENTARY REGULATIONS SIMPLIFIED ACQUISITION PROCEDURES FOR HEALTH-CARE RESOURCES 873.105 Acquisition planning. (a) Acquisition planning is...

  1. 39 CFR 777.41 - Acquisition procedures.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 39 Postal Service 1 2011-07-01 2011-07-01 false Acquisition procedures. 777.41 Section 777.41... ACQUISITION POLICIES Voluntary Acquisitions § 777.41 Acquisition procedures. (a) Voluntary Acquisitions... under § 777.32 of this part, if the acquisition were by eminent domain or the under threat thereof,...

  2. 39 CFR 777.41 - Acquisition procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 39 Postal Service 1 2013-07-01 2013-07-01 false Acquisition procedures. 777.41 Section 777.41... ACQUISITION POLICIES Voluntary Acquisitions § 777.41 Acquisition procedures. (a) Voluntary Acquisitions... under § 777.32 of this part, if the acquisition were by eminent domain or the under threat thereof,...

  3. 39 CFR 777.41 - Acquisition procedures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Acquisition procedures. 777.41 Section 777.41... ACQUISITION POLICIES Voluntary Acquisitions § 777.41 Acquisition procedures. (a) Voluntary Acquisitions... under § 777.32 of this part, if the acquisition were by eminent domain or the under threat thereof,...

  4. 39 CFR 777.41 - Acquisition procedures.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 39 Postal Service 1 2012-07-01 2012-07-01 false Acquisition procedures. 777.41 Section 777.41... ACQUISITION POLICIES Voluntary Acquisitions § 777.41 Acquisition procedures. (a) Voluntary Acquisitions... under § 777.32 of this part, if the acquisition were by eminent domain or the under threat thereof,...

  5. 39 CFR 777.41 - Acquisition procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 39 Postal Service 1 2014-07-01 2014-07-01 false Acquisition procedures. 777.41 Section 777.41... ACQUISITION POLICIES Voluntary Acquisitions § 777.41 Acquisition procedures. (a) Voluntary Acquisitions... under § 777.32 of this part, if the acquisition were by eminent domain or the under threat thereof,...

  6. SNAP: Simulating New Acquisition Processes

    NASA Technical Reports Server (NTRS)

    Alfeld, Louis E.

    1997-01-01

    Simulation models of acquisition processes range in scope from isolated applications to the 'Big Picture' captured by SNAP technology. SNAP integrates a family of models to portray the full scope of acquisition planning and management activities, including budgeting, scheduling, testing and risk analysis. SNAP replicates the dynamic management processes that underlie design, production and life-cycle support. SNAP provides the unique 'Big Picture' capability needed to simulate the entire acquisition process and explore the 'what-if' tradeoffs and consequences of alternative policies and decisions. Comparison of cost, schedule and performance tradeoffs help managers choose the lowest-risk, highest payoff at each step in the acquisition process.

  7. "Data Acquisition Systems"

    SciTech Connect

    Unterweger, Michael; Costrell, Louis deceased

    2009-07-07

    This project involved support for Lou Costrell and myself in the development of IEEE and IEC standards for nuclear counting and data acquisition systems. Over the years, as a result of this support, Lou and I were able to attend standards meetings of IEEE and IEC, which led directly to the publication of many standards for NIM systems, FastBus and CAMAC. We also chaired several writing committees as well as ANSI N42 (Nuclear instrumentation), IEEE NIM (NIM standard), IEEE NID (NPSS nuclear instruments and detector) and IEC TC45 WG9 (Nuclear instrumentation). Through this support we were able to assure that the interests of the US and DOE were expressed and implemented in the various standards.

  8. Plasmid acquisition in microgravity

    NASA Technical Reports Server (NTRS)

    Juergensmeyer, Margaret A.; Juergensmeyer, Elizabeth A.; Guikema, James A.

    1995-01-01

    In microgravity, bacteria often show an increased resistance to antibiotics. Bacteria can develop resistance to an antibiotic after transformation, the acquisition of DNA, usually in the form of a plasmid containing a gene for resistance to one or more antibiotics. In order to study the capacity of bacteria to become resistant to antibiotics in microgravity, we have modified the standard protocol for transformation of Escherichia coli for use in the NASA-flight-certified hardware package, The Fluid Processing Apparatus (FPA). Here we report on the ability of E. coli to remain competent for long periods of time at temperatures that are readily available on the Space Shuttle, and present some preliminary flight results.

  9. Silicon tracker data acquisition

    SciTech Connect

    Haynes, W.J.

    1997-12-31

    Large particle physics experiments are making increasing technological demands on the design and implementation of real-time data acquisition systems. The LHC will have bunch crossing intervals of 25 nanoseconds and detectors, such as CMS, will contain over 10 million electronic channels. Readout systems will need to cope with 100 kHz rates of 1 MByte-sized events. Over 70% of this voluminous flow will stem from silicon tracker and MSGC devices. This paper describes the techniques currently being harnessed from ASIC devices through to modular microprocessor-based architectures around standards such as VMEbus and PCI. In particular, the experiences gained at the HERA H1 experiment are highlighted where many of the key technological concepts have already been im implemented.

  10. DSPS in data acquisition

    SciTech Connect

    Kirsch, M.; Haeupke, T.; Oelschlaeger, R.; Struck, B.

    1997-12-31

    Off-the-shelf and customized DSP boards in different bus architectures are perfectly suited to act as building blocks for flexible and high performance data acquisition systems. Due to their architecture they can be used to enhance the performance of existing equipment as add ons, as state-of-the-art readout controllers, event builders, on-the-fly data formatters and higher level trigger processors. Applications covering the above mentioned fields with Motorolas 96002 HARC DSP in the DESY HERMES and H1 experiments, at the focal plane polarimeter at KVI and the NIST high flux neutron backscattering spectrometer will be presented. Future possibilities with VME, PCI and PMC boards based on Analog Devices SHARC DSP will be discussed. Systems on the base of Texas Instruments TMS320C6X promise to provide unprecedented performance.

  11. 78 FR 49990 - Land Acquisitions: Appeals of Land Acquisition Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-16

    ...: Comments on the proposed rule published May 29, 2013 (78 FR 32214) must be received by September 3, 2013... 25 CFR 151.12 (78 FR 32214). The proposed rule would remove procedural requirements that are no...; Docket ID: BIA-2013-0005] RIN 1076-AF15 Land Acquisitions: Appeals of Land Acquisition Decisions...

  12. 77 FR 75780 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-64; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-21

    ... Acquisition Regulation; Federal Acquisition Circular 2005-64; Small Entity Compliance Guide AGENCIES... the rule appearing in Federal Acquisition Circular (FAC) 2005-64, which amends the Federal...

  13. Knowledge Acquisition in Observational Astronomy.

    ERIC Educational Resources Information Center

    Vosniadou, Stella

    This paper presents findings from research on knowledge acquisition in observational astronomy to demonstrate the kinds of intuitive models children form and to show how these models influence the acquisition of science knowledge. Sixty children of approximate ages 6, 9, and 12 were given a questionnaire to investigate their knowledge of the size,…

  14. STIS CCD Target Acquisition Workout

    NASA Astrophysics Data System (ADS)

    Downes, Ron

    1997-07-01

    Following update of STIS flight acquisitions and acq/peak software, redemonstrate the capability to perform point source and peakup acquisitions of a bright target. Then demonstrate ability of the STIS FSW to locate targets more typical of those in the science program.

  15. New online signature acquisition system

    NASA Astrophysics Data System (ADS)

    Oulefki, Adel; Mostefai, Messaoud; Abbadi, Belkacem; Djebrani, Samira; Bouziane, Abderraouf; Chahir, Youssef

    2013-01-01

    We present a nonconstraining and low-cost online signature acquisition system that has been developed to enhance the performances of an existing multimodal biometric authentication system (based initially on both voice and image modalities). A laboratory prototype has been developed and validated for an online signature acquisition.

  16. Ocean Data Acquisition System

    NASA Technical Reports Server (NTRS)

    Johnson, B.; Cavanaugh, J.; Smith, J.; Esaias, W.

    1988-01-01

    The Ocean Data Acquisition System (ODAS) is a low cost instrument with potential commercial application. It is easily mounted on a small aircraft and flown over the coastal zone ocean to remotely measure sea surface temperature and three channels of ocean color information. From this data, chlorophyll levels can be derived for use by ocean scientists, fisheries, and environmental offices. Data can be transmitted to shipboard for real-time use with sea truth measurements, ocean productivity estimates and fishing fleet direction. The aircraft portion of the system has two primary instruments: an IR radiometer to measure sea surface temperature and a three channel visible spectro-radiometer for 460, 490, and 520 nm wavelength measurements from which chlorophyll concentration can be derived. The aircraft package contains a LORAN-C unit for aircraft location information, clock, on-board data processor and formatter, digital data storage, packet radio terminal controller, and radio transceiver for data transmission to a ship. The shipboard package contains a transceiver, packet terminal controller, data processing and storage capability, and printer. Both raw data and chlorophyll concentrations are available for real-time analysis.

  17. 48 CFR 304.7001 - Numbering acquisitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Numbering acquisitions. 304.7001 Section 304.7001 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATIVE MATTERS Acquisition Instrument Identification Numbering System 304.7001 Numbering acquisitions....

  18. 48 CFR 304.7001 - Numbering acquisitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Numbering acquisitions. 304.7001 Section 304.7001 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATIVE MATTERS Acquisition Instrument Identification Numbering System 304.7001 Numbering acquisitions....

  19. 48 CFR 304.7001 - Numbering acquisitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Numbering acquisitions. 304.7001 Section 304.7001 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATIVE MATTERS Acquisition Instrument Identification Numbering System 304.7001 Numbering acquisitions....

  20. 48 CFR 304.7001 - Numbering acquisitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Numbering acquisitions. 304.7001 Section 304.7001 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATIVE MATTERS Acquisition Instrument Identification Numbering System 304.7001 Numbering acquisitions....

  1. 48 CFR 304.7001 - Numbering acquisitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Numbering acquisitions. 304.7001 Section 304.7001 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATIVE MATTERS Acquisition Instrument Identification Numbering System 304.7001 Numbering acquisitions....

  2. 48 CFR 234.004 - Acquisition strategy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Acquisition strategy. 234..., DEPARTMENT OF DEFENSE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION 234.004 Acquisition strategy. (1) See 209.570 for policy applicable to acquisition strategies that consider the use of lead...

  3. 48 CFR 3034.004 - Acquisition strategy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Acquisition strategy. See (HSAR) 48 CFR 3009.570 for policy applicable to acquisition strategies that consider... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Acquisition strategy. 3034.004 Section 3034.004 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY,...

  4. 48 CFR 34.004 - Acquisition strategy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisition strategy. 34... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 34.004 Acquisition strategy. The program manager, as specified in agency procedures, shall develop an acquisition strategy tailored to the...

  5. 48 CFR 434.004 - Acquisition strategy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Acquisition strategy. 434... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 434.004 Acquisition strategy. (a) The program manager will develop, in coordination with the Acquisition Executive or Major Information...

  6. 48 CFR 3034.004 - Acquisition strategy.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Acquisition strategy. See (HSAR) 48 CFR 3009.570 for policy applicable to acquisition strategies that consider... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Acquisition strategy. 3034.004 Section 3034.004 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY,...

  7. 48 CFR 3034.004 - Acquisition strategy.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Acquisition strategy. See (HSAR) 48 CFR 3009.570 for policy applicable to acquisition strategies that consider... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Acquisition strategy. 3034.004 Section 3034.004 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY,...

  8. 48 CFR 234.004 - Acquisition strategy.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Acquisition strategy. 234..., DEPARTMENT OF DEFENSE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION 234.004 Acquisition strategy. (1) See 209.570 for policy applicable to acquisition strategies that consider the use of lead...

  9. 48 CFR 1034.004 - Acquisition strategy.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Acquisition strategy. 1034... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 1034.004 Acquisition strategy. (a) A program manager's acquisition strategy written at the system or investment level in accordance with FAR...

  10. 48 CFR 434.004 - Acquisition strategy.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Acquisition strategy. 434.004 Section 434.004 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 434.004 Acquisition strategy. (a) The...

  11. 48 CFR 434.004 - Acquisition strategy.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Acquisition strategy. 434.004 Section 434.004 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 434.004 Acquisition strategy. (a) The...

  12. 48 CFR 234.004 - Acquisition strategy.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Acquisition strategy. 234..., DEPARTMENT OF DEFENSE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION 234.004 Acquisition strategy. (1) See 209.570 for policy applicable to acquisition strategies that consider the use of lead...

  13. 48 CFR 3034.004 - Acquisition strategy.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Acquisition strategy. See (HSAR) 48 CFR 3009.570 for policy applicable to acquisition strategies that consider... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Acquisition strategy. 3034.004 Section 3034.004 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY,...

  14. 48 CFR 3034.004 - Acquisition strategy.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Acquisition strategy. See (HSAR) 48 CFR 3009.570 for policy applicable to acquisition strategies that consider... 48 Federal Acquisition Regulations System 7 2011-10-01 2011-10-01 false Acquisition strategy. 3034.004 Section 3034.004 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY,...

  15. 48 CFR 34.004 - Acquisition strategy.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Acquisition strategy. 34... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 34.004 Acquisition strategy. The program manager, as specified in agency procedures, shall develop an acquisition strategy tailored to the...

  16. 48 CFR 1034.004 - Acquisition strategy.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Acquisition strategy. 1034... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 1034.004 Acquisition strategy. (a) A program manager's acquisition strategy written at the system or investment level in accordance with FAR...

  17. 48 CFR 434.004 - Acquisition strategy.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Acquisition strategy. 434.004 Section 434.004 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 434.004 Acquisition strategy. (a) The...

  18. 48 CFR 434.004 - Acquisition strategy.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Acquisition strategy. 434.004 Section 434.004 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 434.004 Acquisition strategy. (a) The...

  19. 48 CFR 34.004 - Acquisition strategy.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Acquisition strategy. 34... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 34.004 Acquisition strategy. The program manager, as specified in agency procedures, shall develop an acquisition strategy tailored to the...

  20. 48 CFR 234.004 - Acquisition strategy.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Acquisition strategy. 234..., DEPARTMENT OF DEFENSE SPECIAL CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION 234.004 Acquisition strategy. (1) See 209.570 for policy applicable to acquisition strategies that consider the use of lead...

  1. 48 CFR 34.004 - Acquisition strategy.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Acquisition strategy. 34... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 34.004 Acquisition strategy. The program manager, as specified in agency procedures, shall develop an acquisition strategy tailored to the...

  2. 48 CFR 1034.004 - Acquisition strategy.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Acquisition strategy. 1034... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 1034.004 Acquisition strategy. (a) A program manager's acquisition strategy written at the system or investment level in accordance with FAR...

  3. 48 CFR 34.004 - Acquisition strategy.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Acquisition strategy. 34... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 34.004 Acquisition strategy. The program manager, as specified in agency procedures, shall develop an acquisition strategy tailored to the...

  4. 48 CFR 1034.004 - Acquisition strategy.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Acquisition strategy. 1034... CATEGORIES OF CONTRACTING MAJOR SYSTEM ACQUISITION General 1034.004 Acquisition strategy. (a) A program manager's acquisition strategy written at the system or investment level in accordance with FAR...

  5. 48 CFR 23.103 - Sustainable acquisitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Sustainable acquisitions... SAFETY, AND DRUG-FREE WORKPLACE Sustainable Acquisition Policy 23.103 Sustainable acquisitions. (a) Federal agencies shall advance sustainable acquisition by ensuring that 95 percent of new contract...

  6. Acquisition of thermotolerance during development of Blastocladiella emersonii.

    PubMed

    Silva, A M; Juliani, M H; da Costa, J J; Bonato, M C

    1987-04-14

    In the fungus Blastocladiella emersonii the synthesis of heat-shock proteins is developmentally regulated; particular subsets of heat-shock proteins are induced by heat shock during sporulation, germination and growth and some heat shock-related proteins are spontaneously expressed during sporulation (Bonato et al., 1987, Eur. J. Biochem., in press). Nevertheless, acquisition of thermotolerance can be induced at any stage of the life cycle. The development of thermotolerance is correlated with the enhanced synthesis of some heat-shock proteins: hsp 82a, hsp 82b, hsp 76, hsp 70, hsp 60, hsp 25, hsp 17b. Other hsps are not specifically involved in thermotolerance. PMID:3579921

  7. Automated ship image acquisition

    NASA Astrophysics Data System (ADS)

    Hammond, T. R.

    2008-04-01

    The experimental Automated Ship Image Acquisition System (ASIA) collects high-resolution ship photographs at a shore-based laboratory, with minimal human intervention. The system uses Automatic Identification System (AIS) data to direct a high-resolution SLR digital camera to ship targets and to identify the ships in the resulting photographs. The photo database is then searchable using the rich data fields from AIS, which include the name, type, call sign and various vessel identification numbers. The high-resolution images from ASIA are intended to provide information that can corroborate AIS reports (e.g., extract identification from the name on the hull) or provide information that has been omitted from the AIS reports (e.g., missing or incorrect hull dimensions, cargo, etc). Once assembled into a searchable image database, the images can be used for a wide variety of marine safety and security applications. This paper documents the author's experience with the practicality of composing photographs based on AIS reports alone, describing a number of ways in which this can go wrong, from errors in the AIS reports, to fixed and mobile obstructions and multiple ships in the shot. The frequency with which various errors occurred in automatically-composed photographs collected in Halifax harbour in winter time were determined by manual examination of the images. 45% of the images examined were considered of a quality sufficient to read identification markings, numbers and text off the entire ship. One of the main technical challenges for ASIA lies in automatically differentiating good and bad photographs, so that few bad ones would be shown to human users. Initial attempts at automatic photo rating showed 75% agreement with manual assessments.

  8. 48 CFR 873.105 - Acquisition planning.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... equipment or space, where the acquisition is expected to exceed the simplified acquisition threshold (SAT... particular acquisition expected to exceed the SAT. The team should consist of a mix of staff, appropriate...

  9. Microcomputer Acquisition Standards and Controls.

    ERIC Educational Resources Information Center

    Wold, Geoffrey H.

    1987-01-01

    Increased use of microcomputers in schools can be implemented more effectively when management develops acquisitions standards and controls. Technical standards as well as operational and documentation standards are outlined. (MLF)

  10. Standard GANIL data acquisition system

    NASA Astrophysics Data System (ADS)

    Raine, B.; Tripon, M.; Piquet, B.

    1994-02-01

    We report on the GANIL general data acquisition system based on VME crates distributed in several experiments areas, linked to a VAX cluster by optical fibers and Ethernet for control and storage. Acquisition buses are CAMAC, FERA and VXI. We present the system configuration, experiments description procedure, and adaptation for VXI and remote controls for the 4 INDRA detector. We also discus the INDRA asynchronous electronics trigger.

  11. Microcomputer data acquisition and control.

    PubMed

    East, T D

    1986-01-01

    In medicine and biology there are many tasks that involve routine well defined procedures. These tasks are ideal candidates for computerized data acquisition and control. As the performance of microcomputers rapidly increases and cost continues to go down the temptation to automate the laboratory becomes great. To the novice computer user the choices of hardware and software are overwhelming and sadly most of the computer sales persons are not at all familiar with real-time applications. If you want to bill your patients you have hundreds of packaged systems to choose from; however, if you want to do real-time data acquisition the choices are very limited and confusing. The purpose of this chapter is to provide the novice computer user with the basics needed to set up a real-time data acquisition system with the common microcomputers. This chapter will cover the following issues necessary to establish a real time data acquisition and control system: Analysis of the research problem: Definition of the problem; Description of data and sampling requirements; Cost/benefit analysis. Choice of Microcomputer hardware and software: Choice of microprocessor and bus structure; Choice of operating system; Choice of layered software. Digital Data Acquisition: Parallel Data Transmission; Serial Data Transmission; Hardware and software available. Analog Data Acquisition: Description of amplitude and frequency characteristics of the input signals; Sampling theorem; Specification of the analog to digital converter; Hardware and software available; Interface to the microcomputer. Microcomputer Control: Analog output; Digital output; Closed-Loop Control. Microcomputer data acquisition and control in the 21st Century--What is in the future? High speed digital medical equipment networks; Medical decision making and artificial intelligence. PMID:3805859

  12. 48 CFR 7.402 - Acquisition methods.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time...

  13. 39 CFR 777.31 - Acquisition procedures.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 39 Postal Service 1 2012-07-01 2012-07-01 false Acquisition procedures. 777.31 Section 777.31... ACQUISITION POLICIES Acquisition § 777.31 Acquisition procedures. (a) Policy; Application of Section. The... the initiation of negotiations (see § 777.13(k) for definition) the Postal Service must establish...

  14. 39 CFR 777.31 - Acquisition procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 39 Postal Service 1 2013-07-01 2013-07-01 false Acquisition procedures. 777.31 Section 777.31... ACQUISITION POLICIES Acquisition § 777.31 Acquisition procedures. (a) Policy; Application of Section. The... the initiation of negotiations (see § 777.13(k) for definition) the Postal Service must establish...

  15. 39 CFR 777.31 - Acquisition procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 39 Postal Service 1 2014-07-01 2014-07-01 false Acquisition procedures. 777.31 Section 777.31... ACQUISITION POLICIES Acquisition § 777.31 Acquisition procedures. (a) Policy; Application of Section. The... the initiation of negotiations (see § 777.13(k) for definition) the Postal Service must establish...

  16. 48 CFR 7.402 - Acquisition methods.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time...

  17. 48 CFR 7.402 - Acquisition methods.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time...

  18. 48 CFR 7.402 - Acquisition methods.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Acquisition methods. 7.402... ACQUISITION PLANNING Equipment Lease or Purchase 7.402 Acquisition methods. (a) Purchase method. (1) Generally, the purchase method is appropriate if the equipment will be used beyond the point in time...

  19. Iron Acquisition and Regulation in Campylobacter jejuni

    PubMed Central

    Palyada, Kiran; Threadgill, Deborah; Stintzi, Alain

    2004-01-01

    Iron affects the physiology of bacteria in two different ways: as a micronutrient for bacterial growth and as a catalyst for the formation of hydroxyl radicals. In this study, we used DNA microarrays to identify the C. jejuni genes that have their transcript abundance affected by iron availability. The transcript levels of 647 genes were affected after the addition of iron to iron-limited C. jejuni cells. Several classes of affected genes were revealed within 15 min, including immediate-early response genes as well as those specific to iron acquisition and metabolism. In contrast, only 208 genes were differentially expressed during steady-state experiments comparing iron-rich and iron-limited growth conditions. As expected, genes annotated as being involved in either iron acquisition or oxidative stress defense were downregulated during both time course and steady-state experiments, while genes encoding proteins involved in energy metabolism were upregulated. Because the level of protein glycosylation increased with iron limitation, iron may modulate the level of C. jejuni virulence by affecting the degree of protein glycosylation. Since iron homeostasis has been shown to be Fur regulated in C. jejuni, an isogenic fur mutant was used to define the Fur regulon by transcriptome profiling. A total of 53 genes were Fur regulated, including many genes not previously associated with Fur regulation. A putative Fur binding consensus sequence was identified in the promoter region of most iron-repressed and Fur-regulated genes. Interestingly, a fur mutant was found to be significantly affected in its ability to colonize the gastrointestinal tract of chicks, highlighting the importance of iron homeostasis in vivo. Directed mutagenesis of other genes identified by the microarray analyses allowed the characterization of the ferric enterobactin receptor, previously named CfrA. Chick colonization assays indicated that mutants defective in enterobactin-mediated iron acquisition

  20. Multiplexed quantification for data-independent acquisition.

    PubMed

    Minogue, Catherine E; Hebert, Alexander S; Rensvold, Jarred W; Westphall, Michael S; Pagliarini, David J; Coon, Joshua J

    2015-03-01

    Data-independent acquisition (DIA) strategies provide a sensitive and reproducible alternative to data-dependent acquisition (DDA) methods for large-scale quantitative proteomic analyses. Unfortunately, DIA methods suffer from incompatibility with common multiplexed quantification methods, specifically stable isotope labeling approaches such as isobaric tags and stable isotope labeling of amino acids in cell culture (SILAC). Here we expand the use of neutron-encoded (NeuCode) SILAC to DIA applications (NeuCoDIA), producing a strategy that enables multiplexing within DIA scans without further convoluting the already complex MS(2) spectra. We demonstrate duplex NeuCoDIA analysis of both mixed-ratio (1:1 and 10:1) yeast and mouse embryo myogenesis proteomes. Analysis of the mixed-ratio yeast samples revealed the strong accuracy and precision of our NeuCoDIA method, both of which were comparable to our established MS(1)-based quantification approach. NeuCoDIA also uncovered the dynamic protein changes that occur during myogenic differentiation, demonstrating the feasibility of this methodology for biological applications. We consequently establish DIA quantification of NeuCode SILAC as a useful and practical alternative to DDA-based approaches. PMID:25621425

  1. Multiplexed Quantification for Data-Independent Acquisition

    PubMed Central

    Minogue, Catherine E.; Hebert, Alexander S.; Rensvold, Jarred W.; Westphall, Michael S.; Pagliarini, David J.; Coon, Joshua J.

    2015-01-01

    Data-independent acquisition (DIA) strategies provide a sensitive and reproducible alternative to data-dependent acquisition (DDA) methods for large-scale quantitative proteomic analyses. Unfortunately, DIA methods suffer from incompatibility with common multiplexed quantification methods, specifically stable isotope labeling approaches such as isobaric tags and stable isotope labeling of amino acids in cell culture (SILAC). Here we expand the use of neutron-encoded (NeuCode) SILAC to DIA applications (NeuCoDIA), producing a strategy that enables multiplexing within DIA scans without further convoluting the already complex MS2 spectra. We demonstrate duplex NeuCoDIA analysis of both mixed-ratio (1:1 and 10:1) yeast and mouse embryo myogenesis proteomes. Analysis of the mixed-ratio yeast samples revealed the strong accuracy and precision of our NeuCoDIA method, both of which were comparable to our established MS1-based quantification approach. NeuCoDIA also uncovered the dynamic protein changes that occur during myogenic differentiation, demonstrating the feasibility of this methodology for biological applications. We consequently establish DIA quantification of NeuCode SILAC as a useful and practical alternative to DDA-based approaches. PMID:25621425

  2. 78 FR 67928 - Land Acquisitions: Appeals of Land Acquisition Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... Secretary acquired title to land in trust. See 61 FR 18082 (Apr. 24, 1996). The Department had determined... for issuing trust acquisition decisions. 78 FR 32214. BIA then extended the original comment deadline of July 29, 2013 to September 3, 2013. See 78 FR 49990 (Aug. 16, 2013). Following tribal...

  3. 48 CFR Appendix - Federal Acquisition Regulation (FAR) Index

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Federal Acquisition Regulation (FAR) Index Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT... (IFMS) Contract clause. FAR Index Federal Acquisition Regulation (FAR) Index Editorial Note:...

  4. 48 CFR Appendix - Federal Acquisition Regulation (FAR) Index

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Federal Acquisition Regulation (FAR) Index Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT... (IFMS) Contract clause. FAR Index Federal Acquisition Regulation (FAR) Index Editorial Note:...

  5. 48 CFR Appendix - Federal Acquisition Regulation (FAR) Index

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Federal Acquisition Regulation (FAR) Index Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT... (IFMS) Contract clause. FAR Index Federal Acquisition Regulation (FAR) Index Editorial Note:...

  6. Patron-Driven Ebook Acquisition

    ERIC Educational Resources Information Center

    Breitbach, William; Lambert, Joy E.

    2011-01-01

    Selecting the most appropriate content for a community is a challenge librarians have faced since the dawn of libraries. Formal patron-driven acquisition (PDA) models have been around for at least a decade. These models attempt to collect materials based on known patron demand. Some libraries simply use a suggestion form, while others use…

  7. Causal Models of Literacy Acquisition.

    ERIC Educational Resources Information Center

    Goetz, Ernest T.; And Others

    1992-01-01

    Examines seven articles that employed path analysis to test causal models of the acquisition of literacy or the reading-writing relationship. Reveals that, although such analysis holds promise for a better understanding of the components of literacy, several potential difficulties remain for those attempting to synthesize this body of literature.…

  8. Acquisition streamlining: A cultural change

    NASA Technical Reports Server (NTRS)

    Stewart, Jesse

    1992-01-01

    The topics are presented in viewgraph form and include the following: the defense systems management college, educational philosophy, the defense acquisition environment, streamlining initiatives, organizational streamlining types, defense law review, law review purpose, law review objectives, the Public Law Pilot Program, and cultural change.

  9. Bilingualism and Third Language Acquisition.

    ERIC Educational Resources Information Center

    Garate, Jose Valencia; Iragui, Jasone Cenoz

    A study investigated the role of bilingualism (Basque/Spanish) and motivation in third (English) language acquisition in Spain's Basque country. Subjects were 321 secondary school students in two programs, one with instruction primarily in Spanish and one with instruction primarily in Basque. The following independent variables were analyzed in…

  10. Language Acquisition and Language Revitalization

    ERIC Educational Resources Information Center

    O'Grady, William; Hattori, Ryoko

    2016-01-01

    Intergenerational transmission, the ultimate goal of language revitalization efforts, can only be achieved by (re)establishing the conditions under which an imperiled language can be acquired by the community's children. This paper presents a tutorial survey of several key points relating to language acquisition and maintenance in children,…

  11. Hospital Acquisitions Before Healthcare Reform.

    PubMed

    McCue, Michael J; Thompson, Jon M; Kim, Tae Hyun

    2015-01-01

    The hospital industry has experienced increased consolidation in the past 20 years. Since 2010, in particular, there has been a large rise in the number of hospital acquisitions, and observers have suggested this is due in part to the expected impact of federal healthcare reform legislation. This article reports on a study undertaken to identify the market, management, and financial factors affecting acute care, community hospitals acquired between 2010 and 2012. We identified 77 such hospitals and compared them to other acute care facilities. To assess how different factors were associated with acquisitions, the study used multiple logistic regressions whereby market factors were included first, followed by management and financial factors. Study findings show that acquired hospitals were located in markets with lower rates of preferred provider organization (PPO) penetration compared with nonacquired hospitals. Occupancy rate was found to be inversely related to acquisition rate; however, case-mix index was significantly and positively related to a hospital's being acquired. Financial factors negatively associated with a hospital's being acquired included age of plant and cash flow margin. In contrast to the findings from earlier studies of hospital acquisitions, our results showed that acquired hospitals possessed newer assets. However, similar to the findings of other studies, the cash flow margin of acquired hospitals was lower than that of nonacquired facilities. PMID:26554263

  12. Literacy Acquisition in Deaf Children.

    ERIC Educational Resources Information Center

    Evans, Charlotte

    A review of literature focuses on the literacy acquisition process of deaf children who acquire American Sign Language (ASL) as a first language and written English as a second language. Literacy in this context is defined broadly to include the context and culture in which reading and writing occur, referring to the strong connection between…

  13. Acquisition streamlining: A cultural change

    NASA Astrophysics Data System (ADS)

    Stewart, Jesse

    The topics are presented in viewgraph form and include the following: the defense systems management college, educational philosophy, the defense acquisition environment, streamlining initiatives, organizational streamlining types, defense law review, law review purpose, law review objectives, the Public Law Pilot Program, and cultural change.

  14. The Acquisition of [Head] Movement.

    ERIC Educational Resources Information Center

    Pye, Clifton

    An analysis of one theory of the acquisition of head movement by children is presented, using longitudinal data from the Mayan language, K'iche'. This theory assumes that children would just require positive evidence of head movement in the input language to instantiate the constructions of their own grammar. The Incorporation Theory addresses the…

  15. Analog Input Data Acquisition Software

    NASA Technical Reports Server (NTRS)

    Arens, Ellen

    2009-01-01

    DAQ Master Software allows users to easily set up a system to monitor up to five analog input channels and save the data after acquisition. This program was written in LabVIEW 8.0, and requires the LabVIEW runtime engine 8.0 to run the executable.

  16. Soviet Studies in Language Acquisition

    ERIC Educational Resources Information Center

    Smith, Paul R., Jr.

    1973-01-01

    Article surveys the special theories and empirical studies of language acquisition carried out by Soviet psychologists, especially in the period since 1950. The basic principles of materialism, historicism and social reference characteristic of Soviet Marxist psychology are placed in contrast to certain current tendencies in American linguistics.…

  17. 78 FR 38537 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-68; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-26

    ... Acquisition Regulation; Federal Acquisition Circular 2005-68; Small Entity Compliance Guide AGENCY: Department... the rule appearing in Federal Acquisition Circular (FAC) 2005-68, which amends the Federal...

  18. 77 FR 27551 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-59; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... Acquisition Regulation; Federal Acquisition Circular 2005-59; Small Entity Compliance Guide AGENCY: Department... the rule appearing in Federal Acquisition Circular (FAC) 2005-59, which amends the Federal...

  19. 75 FR 39420 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-44; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-08

    ... Acquisition Regulation; Federal Acquisition Circular 2005-44; Small Entity Compliance Guide AGENCY: Department... the summary of the rule appearing in Federal Acquisition Circular (FAC) 2005-44 which amends...

  20. Buffer Gas Acquisition and Storage

    NASA Technical Reports Server (NTRS)

    Parrish, Clyde F.; Lueck, Dale E.; Jennings, Paul A.; Callahan, Richard A.; Delgado, H. (Technical Monitor)

    2001-01-01

    The acquisition and storage of buffer gases (primarily argon and nitrogen) from the Mars atmosphere provides a valuable resource for blanketing and pressurizing fuel tanks and as a buffer gas for breathing air for manned missions. During the acquisition of carbon dioxide (CO2), whether by sorption bed or cryo-freezer, the accompanying buffer gases build up in the carbon dioxide acquisition system, reduce the flow of CO2 to the bed, and lower system efficiency. It is this build up of buffer gases that provide a convenient source, which must be removed, for efficient capture Of CO2 Removal of this buffer gas barrier greatly improves the charging rate of the CO2 acquisition bed and, thereby, maintains the fuel production rates required for a successful mission. Consequently, the acquisition, purification, and storage of these buffer gases are important goals of ISRU plans. Purity of the buffer gases is a concern e.g., if the CO, freezer operates at 140 K, the composition of the inert gas would be approximately 21 percent CO2, 50 percent nitrogen, and 29 percent argon. Although there are several approaches that could be used, this effort focused on a hollow-fiber membrane (HFM) separation method. This study measured the permeation rates of CO2, nitrogen (ND, and argon (Ar) through a multiple-membrane system and the individual membranes from room temperature to 193K and 10 kpa to 300 kPa. Concentrations were measured with a gas chromatograph that used a thermoconductivity (TCD) detector with helium (He) as the carrier gas. The general trend as the temperature was lowered was for the membranes to become more selective, In addition, the relative permeation rates between the three gases changed with temperature. The end result was to provide design parameters that could be used to separate CO2 from N2 and Ar.

  1. Data Acquisition for Modular Biometric Monitoring System

    NASA Technical Reports Server (NTRS)

    Chmiel, Alan J. (Inventor); Humphreys, Bradley T. (Inventor); Grodsinsky, Carlos M. (Inventor)

    2014-01-01

    A modular system for acquiring biometric data includes a plurality of data acquisition modules configured to sample biometric data from at least one respective input channel at a data acquisition rate. A representation of the sampled biometric data is stored in memory of each of the plurality of data acquisition modules. A central control system is in communication with each of the plurality of data acquisition modules through a bus. The central control system is configured to collect data asynchronously, via the bus, from the memory of the plurality of data acquisition modules according to a relative fullness of the memory of the plurality of data acquisition modules.

  2. 76 FR 10476 - (General Provisions) Contract Appeals and the Acquisition Regulation: General, Acquisition...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-25

    ... 1991-AB81 (General Provisions) Contract Appeals and the Acquisition Regulation: General, Acquisition Planning, and Contracting Methods and Contract Types Correction In rule document 2011-1320 appearing...

  3. 48 CFR 307.104-70 - Acquisition strategy.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Acquisition strategy. 307.104-70 Section 307.104-70 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES COMPETITION AND ACQUISITION PLANNING ACQUISITION PLANNING Acquisition Planning 307.104-70 Acquisition strategy. Program and Project Managers responsible...

  4. 48 CFR 307.104-70 - Acquisition strategy.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Acquisition strategy. 307... AND ACQUISITION PLANNING ACQUISITION PLANNING Acquisition Planning 307.104-70 Acquisition strategy... designated by the HHS CIO, DASFMP, the CAO, or the cognizant HCA) shall prepare an acquisition strategy...

  5. 48 CFR 307.104-70 - Acquisition strategy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Acquisition strategy. 307... AND ACQUISITION PLANNING ACQUISITION PLANNING Acquisition Planning 307.104-70 Acquisition strategy... designated by the HHS CIO, DASFMP, the CAO, or the cognizant HCA) shall prepare an acquisition strategy...

  6. 48 CFR 307.104-70 - Acquisition strategy.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Acquisition strategy. 307... AND ACQUISITION PLANNING ACQUISITION PLANNING Acquisition Planning 307.104-70 Acquisition strategy... designated by the HHS CIO, DASFMP, the CAO, or the cognizant HCA) shall prepare an acquisition strategy...

  7. 48 CFR 307.104-70 - Acquisition strategy.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Acquisition strategy. 307... AND ACQUISITION PLANNING ACQUISITION PLANNING Acquisition Planning 307.104-70 Acquisition strategy... designated by the HHS CIO, DASFMP, the CAO, or the cognizant HCA) shall prepare an acquisition strategy...

  8. 32 CFR 644.7 - Acquisition lines.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... HANDBOOK Project Planning Civil Works § 644.7 Acquisition lines. (a) Tentative acquisition lines. As... submission may be for an entire project or by segments or units. However, if the final map is submitted on...

  9. Music and Early Language Acquisition

    PubMed Central

    Brandt, Anthony; Gebrian, Molly; Slevc, L. Robert

    2012-01-01

    Language is typically viewed as fundamental to human intelligence. Music, while recognized as a human universal, is often treated as an ancillary ability – one dependent on or derivative of language. In contrast, we argue that it is more productive from a developmental perspective to describe spoken language as a special type of music. A review of existing studies presents a compelling case that musical hearing and ability is essential to language acquisition. In addition, we challenge the prevailing view that music cognition matures more slowly than language and is more difficult; instead, we argue that music learning matches the speed and effort of language acquisition. We conclude that music merits a central place in our understanding of human development. PMID:22973254

  10. 4 NCIC Data Acquisition Guide

    USGS Publications Warehouse

    U.S. Geological Survey

    1979-01-01

    The National Cartographic Information Center (NCIC) is the information branch of the National Mapping Program. NCIC gathers descriptions of cartographic products held by Federal, State and local agencies, and private companies. Computerized information systems are used to edit, store, and distribute these descriptions. The Aerial Photography Summary Record System (APSRS) is NCIC's system for determining if photographs of a particular area, with a particular set of characteristics, are available, and if so, where they can be found. This guide defines the procedures for planning and conducting visits to potential contributors to the APSRS data base. The guide also defines acquisition responsibilities of the contributor, State affiliates, mapping centers, and NCIC headquarters. As a Data Acquisition specialist, you are encouraged to seek innovative approaches to acquiring data and to suggest additions or changes to this guide.

  11. Centaur propellant acquisition system study

    NASA Technical Reports Server (NTRS)

    Blatt, M. H.; Walter, M. D.

    1975-01-01

    A study was performed to determine the desirability of replacing the hydrogen peroxide settling system on the Centaur D-1S with a capillary acquisition system. A comprehensive screening was performed to select the most promising capillary device fluid acquisition, thermal conditioning, and fabrication techniques. Refillable start baskets and bypass feed start tanks were selected for detailed design. Critical analysis areas were settling and refilling, start sequence development with an initially dry boost pump, and cooling the fluid delivered to the boost pump in order to provide necessary net position suction head (NPSH). Design drawings were prepared for the start basket and start tank concepts for both LO2 and LH2 tanks. System comparisons indicated that the start baskets using wicking for thermal conditioning, and thermal subcooling for boost pump NPSH, are the most desirable systems for future development.

  12. Fast Access Data Acquisition System

    SciTech Connect

    Dr. Vladimir Katsman

    1998-03-17

    Our goal in this program is to develop Fast Access Data Acquisition System (FADAS) by combining the flexibility of Multilink's GaAs and InP electronics and electro-optics with an extremely high data rate for the efficient handling and transfer of collider experimental data. This novel solution is based on Multilink's and Los Alamos National Laboratory's (LANL) unique components and technologies for extremely fast data transfer, storage, and processing.

  13. Aerial robotic data acquisition system

    SciTech Connect

    Hofstetter, K.J.; Hayes, D.W.; Pendergast, M.M.; Corban, J.E.

    1993-12-31

    A small, unmanned aerial vehicle (UAV), equipped with sensors for physical and chemical measurements of remote environments, is described. A miniature helicopter airframe is used as a platform for sensor testing and development. The sensor output is integrated with the flight control system for real-time, interactive, data acquisition and analysis. Pre-programmed flight missions will be flown with several sensors to demonstrate the cost-effective surveillance capabilities of this new technology.

  14. WRATS Integrated Data Acquisition System

    NASA Technical Reports Server (NTRS)

    Piatak, David J.

    2008-01-01

    The Wing and Rotor Aeroelastic Test System (WRATS) data acquisition system (DAS) is a 64-channel data acquisition display and analysis system specifically designed for use with the WRATS 1/5-scale V-22 tiltrotor model of the Bell Osprey. It is the primary data acquisition system for experimental aeroelastic testing of the WRATS model for the purpose of characterizing the aeromechanical and aeroelastic stability of prototype tiltrotor configurations. The WRATS DAS was also used during aeroelastic testing of Bell Helicopter Textron s Quad-Tiltrotor (QTR) design concept, a test which received international attention. The LabVIEW-based design is portable and capable of powering and conditioning over 64 channels of dynamic data at sampling rates up to 1,000 Hz. The system includes a 60-second circular data archive, an integrated model swashplate excitation system, a moving block damping application for calculation of whirl flutter mode subcritical damping, a loads and safety monitor, a pilot-control console display, data analysis capabilities, and instrumentation calibration functions. Three networked computers running custom-designed LabVIEW software acquire data through National Instruments data acquisition hardware. The aeroelastic model (see figure) was tested with the DAS at two facilities at NASA Langley, the Transonic Dynamics Tunnel (TDT) and the Rotorcraft Hover Test Facility (RHTF). Because of the need for seamless transition between testing at these facilities, DAS is portable. The software is capable of harmonic analysis of periodic time history data, Fast Fourier Transform calculations, power spectral density calculations, and on-line calibration of test instrumentation. DAS has a circular buffer archive to ensure critical data is not lost in event of model failure/incident, as well as a sample-and-hold capability for phase-correct time history data.

  15. Enhanced Data-Acquisition System

    NASA Technical Reports Server (NTRS)

    Mustain, Roy W.

    1990-01-01

    Time-consuming, costly digitization of analog signals on magnetic tape eliminated. Proposed data-acquisition system provides nearly immediate access to data in incoming signals by digitizing and recording them both on magnetic tape and on optical disk. Tape and/or disk later played back to reconstruct signals in analog or digital form for analysis. Of interest in industrial and scientific applications in which necessary to digitize, store, and/or process large quantities of experimental data.

  16. Jefferson Lab's Distributed Data Acquisition

    SciTech Connect

    Trent Allison; Thomas Powers

    2006-05-01

    Jefferson Lab's Continuous Electron Beam Accelerator Facility (CEBAF) occasionally experiences fast intermittent beam instabilities that are difficult to isolate and result in downtime. The Distributed Data Acquisition (Dist DAQ) system is being developed to detect and quickly locate such instabilities. It will consist of multiple Ethernet based data acquisition chassis distributed throughout the seven-eights of a mile CEBAF site. Each chassis will monitor various control system signals that are only available locally and/or monitored by systems with small bandwidths that cannot identify fast transients. The chassis will collect data at rates up to 40 Msps in circular buffers that can be frozen and unrolled after an event trigger. These triggers will be derived from signals such as periodic timers or accelerator faults and be distributed via a custom fiber optic event trigger network. This triggering scheme will allow all the data acquisition chassis to be triggered simultaneously and provide a snapshot of relevant CEBAF control signals. The data will then be automatically analyzed for frequency content and transients to determine if and where instabilities exist.

  17. Confidence-Based Feature Acquisition

    NASA Technical Reports Server (NTRS)

    Wagstaff, Kiri L.; desJardins, Marie; MacGlashan, James

    2010-01-01

    Confidence-based Feature Acquisition (CFA) is a novel, supervised learning method for acquiring missing feature values when there is missing data at both training (learning) and test (deployment) time. To train a machine learning classifier, data is encoded with a series of input features describing each item. In some applications, the training data may have missing values for some of the features, which can be acquired at a given cost. A relevant JPL example is that of the Mars rover exploration in which the features are obtained from a variety of different instruments, with different power consumption and integration time costs. The challenge is to decide which features will lead to increased classification performance and are therefore worth acquiring (paying the cost). To solve this problem, CFA, which is made up of two algorithms (CFA-train and CFA-predict), has been designed to greedily minimize total acquisition cost (during training and testing) while aiming for a specific accuracy level (specified as a confidence threshold). With this method, it is assumed that there is a nonempty subset of features that are free; that is, every instance in the data set includes these features initially for zero cost. It is also assumed that the feature acquisition (FA) cost associated with each feature is known in advance, and that the FA cost for a given feature is the same for all instances. Finally, CFA requires that the base-level classifiers produce not only a classification, but also a confidence (or posterior probability).

  18. Undergraduate Laboratory Data Acquisition With a Microcomputer.

    ERIC Educational Resources Information Center

    Weston, Kenneth C.

    1981-01-01

    Describes a flexible, multichannel, highly accurate, digital data acquisition system for use with a microcomputer. Includes a description of instrumentation for computer data acquisition, data acquisition systems, software, uses in the curriculum, library interaction and action, and use in a mechanical engineering laboratory. (DS)

  19. Chain Shift in Second Language Phonological Acquisition.

    ERIC Educational Resources Information Center

    Lee, Shin-Sook

    2000-01-01

    Examines whether Korean students learning English as a Second Language show chain shift of a similar kind to that demonstrated in first language acquisition, and if so, whether there is any difference between first language acquisition and second language acquisition with respect to affected sounds. (Author/VWL)

  20. 14 CFR 151.73 - Land acquisition.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition...

  1. 14 CFR 151.73 - Land acquisition.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition of land or any interest therein, or of...

  2. 14 CFR 151.73 - Land acquisition.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition...

  3. 14 CFR 151.73 - Land acquisition.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition...

  4. 14 CFR 151.73 - Land acquisition.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Land acquisition. 151.73 Section 151.73 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.73 Land acquisition. (a) The acquisition...

  5. 78 FR 73450 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-06

    ... Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations System... Federal Acquisition Regulation Supplement (DFARS) to provide needed editorial changes. DATES:...

  6. 78 FR 73472 - Defense Federal Acquisition Regulation Supplement: Application of Certain Clauses to Acquisitions...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-06

    ... Acquisition Regulation Supplement: Application of Certain Clauses to Acquisitions of Commercial Items (DFARS... (DFARS) to clarify the applicability of two clauses to acquisitions of commercial items. DATES: Comment..., Acquisition of Commercial Items, to clarify the applicability of DFARS 252.211-7008, Use of...

  7. 77 FR 56737 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-61; Introduction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ...This document summarizes the Federal Acquisition Regulation (FAR) rules agreed to by the Civilian Agency Acquisition Council and the Defense Acquisition Regulations Council (Councils) in this Federal Acquisition Circular (FAC) 2005-61. A companion document, the Small Entity Compliance Guide (SECG), follows this FAC. The FAC, including the SECG, is available via the Internet at......

  8. 76 FR 18324 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-51; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... Acquisition Regulation; Federal Acquisition Circular 2005-51; Small Entity Compliance Guide AGENCIES... rules appearing in Federal Acquisition Circular (FAC) 2005-51, which amend the Federal Acquisition...-51, which precedes this document. These documents are also available via the Internet at...

  9. SILEX acquisition and tracking sensors

    NASA Astrophysics Data System (ADS)

    Cockshott, Robert A.; Purll, David J.

    1995-04-01

    This paper describes the Acquisition Sensor Detection Unit (ASDU) and the Tracking Sensor Detection Unit (TSDU) developed by Sira Ltd to be flown in the European Space Agency's SILEX (Semi-conductor Inter satellite Link Experiment) terminals. These units were designed, manufactured and qualified by Sira under contract to Matra Marconi Space. Stringent vibration, thermal stability and video waveform noise and timing specifications were critical elements in the design. The ASDU consists of an optical head containing a 385 X 288 CCD and video buffer, and separate electronics box containing clock decoding electronics, CCD clock drivers and video amplifiers. The ASDU operates at a frame rate of 33 or 135 Hz and its noise performance allows the brightest pixel to be detected with an accuracy of +/- 0.5 pixel for input powers in the range 9 - 55 pW (33 Hz) or 16 - 240 pW (135 Hz) during acquisition. Using the ASDU signal, the SILEX pointing, acquisition and tracking system controls the pointing mirrors to centralize the beam, at which point it falls within the smaller TSDU field of view. The TSDU comprises a single 480 g unit containing a 43 mm focal length lens, a 14 X 14 CCD, and clock receiver and video amplifier electronics. During tracking the central 4 CCD pixels are used as a quadrant detector, and the TSDU has demonstrated a noise equivalent angle of 2.7 (mu) rad (1 (sigma) half cone, 180 pW optical input power) at a frame rate of 8 kHz.

  10. Subsea control and data acquisition

    SciTech Connect

    Not Available

    1990-01-01

    This book contains papers presented at the conference on subsea control and data acquisition. The tasks performed by subsea control systems, although fairly straightforward, are dominated by an alien environment and the high costs associated with failure and repair. The evolution of systems design has resulted in a growing confidence in equipment reliability. In the future, however, more searching demands may include active regulation of valves and the use of data for reservoir management purposes. The emphasis of the papers is on technical depth and understanding, with particular attention being paid to the science control and data monitoring in relation to the task of hydrocarbon recovery.

  11. The Telecommunications and Data Acquisition

    NASA Technical Reports Server (NTRS)

    Posner, Edward C. (Editor)

    1992-01-01

    This quarterly publication provides archival reports on developments in programs managed by JPL's Office of Telecommunications and Data Acquisition (TDA). In space communications, radio navigation, radio science, and ground-based radio and radar astronomy, it reports on activities of the Deep Space Network (DSN) in planning, supporting research and technology, implementation, and operations. Also included are standards activity at JPL for space data and information systems and reimbursable DSN work performed for other space agencies through NASA. The preceding work is all performed for NASA's Office of Space Communications (OSC).

  12. DEAP-3600 Data Acquisition System

    NASA Astrophysics Data System (ADS)

    Lindner, Thomas

    2015-12-01

    DEAP-3600 is a dark matter experiment using liquid argon to detect Weakly Interacting Massive Particles (WIMPs). The DEAP-3600 Data Acquisition (DAQ) has been built using a combination of commercial and custom electronics, organized using the MIDAS framework. The DAQ system needs to suppress a high rate of background events from 39Ar beta decays. This suppression is implemented using a combination of online firmware and software-based event filtering. We will report on progress commissioning the DAQ system, as well as the development of the web-based user interface.

  13. Sample acquisition and instrument deployment

    NASA Technical Reports Server (NTRS)

    Boyd, Robert C.

    1995-01-01

    Progress is reported in developing the Sample Acquisition and Instrument Deployment (SAID) system, a robotic system for deploying science instruments and acquiring samples for analysis. The system is a conventional four degree of freedom manipulator 2 meters in length. A baseline design has been achieved through analysis and trade studies. The design considers environmental operating conditions on the surface of Mars, as well as volume constraints on proposed Mars landers. Control issues have also been studied, and simulations of joint and tip movements have been performed. The systems have been fabricated and tested in environmental chambers, as well as soil testing and robotic control testing.

  14. On acquisition of programming knowledge

    NASA Technical Reports Server (NTRS)

    Amin, Ashok T.

    1987-01-01

    For the evolving discipline of programming, acquisition of programming knowledge is a difficult issue. Common knowledge results from the acceptance of proven techniques based on results of formal inquiries into the nature of the programming process. This is a rather slow process. In addition, the vast body of common knowledge needs to be explicated to a low enough level of details for it to be represented in the machine processable form. It is felt that this is an impediment to the progress of automatic programming. The importance of formal approaches cannot be overstated since their contributions lead to quantum leaps in the state of the art.

  15. Data acquisition system for SLD

    SciTech Connect

    Sherden, D.J.

    1985-05-01

    This paper describes the data acquisition system planned for the SLD detector which is being constructed for use with the SLAC Linear Collider (SLC). An exclusively FASTBUS front-end system is used together with a VAX-based host system. While the volume of data transferred does not challenge the band-width capabilities of FASTBUS, extensive use is made of the parallel processing capabilities allowed by FASTBUS to reduce the data to a size which can be handled by the host system. The low repetition rate of the SLC allows a relatively simple software-based trigger. The principal components and overall architecture of the hardware and software are described.

  16. The NIFFTE Data Acquisition System

    NASA Astrophysics Data System (ADS)

    Qu, Hai; Niffte Collaboration

    2011-10-01

    The Neutron Induced Fission Fragment Tracking Experiment (NIFFTE) will employ a novel, high granularity, pressurized Time Projection Chamber to measure fission cross-sections of the major actinides to high precision over a wide incident neutron energy range. These results will improve nuclear data accuracy and benefit the fuel cycle in the future. The NIFFTE data acquisition system (DAQ) has been designed and implemented on the prototype TPC. Lessons learned from engineering runs have been incorporated into some design changes that are being implemented before the next run cycle. A fully instrumented sextant of EtherDAQ cards (16 sectors, 496 channels) will be used for the next run cycle. The Maximum Integrated Data Acquisition System (MIDAS) has been chosen and customized to configure and run the experiment. It also meets the requirement for remote control and monitoring of the system. The integration of the MIDAS online database with the persistent PostgreSQL database has been implemented for experiment usage. The detailed design and current status of the DAQ system will be presented.

  17. Rotary Percussive Sample Acquisition Tool

    NASA Technical Reports Server (NTRS)

    Klein, K.; Badescu, M.; Haddad, N.; Shiraishi, L.; Walkemeyer, P.

    2012-01-01

    As part of a potential Mars Sample Return campaign NASA is studying a sample caching mission to Mars, with a possible 2018 launch opportunity. As such, a Sample Acquisition Tool (SAT) has been developed in support of the Integrated Mars Sample Acquisition and Handling (IMSAH) architecture as it relates to the proposed Mars Sample Return (MSR) campaign. The tool allows for core generation and capture directly into a sample tube. In doing so, the sample tube becomes the fundamental handling element within the IMSAH sample chain reducing the risk associated with sample contamination as well as the need to handle a sample of unknown geometry. The tool's functionality was verified utilizing a proposed rock test suite that encompasses a series of rock types that have been utilized in the past to qualify Martian surface sampling hardware. The corresponding results have shown the tool can effectively generate, fracture, and capture rock cores while maintaining torque margins of no less than 50% with an average power consumption of no greater than 90W and a tool mass of less than 6kg.

  18. Atmospheric effects on target acquisition

    NASA Astrophysics Data System (ADS)

    Kopeika, Norman S.; Zilberman, Arkadi; Yitzhaky, Yitzhak; Golbraikh, Ephim

    2012-06-01

    Imaging systems have advanced significantly in the last decades in terms of low noise and better resolution. While imaging hardware resolution can be limited by collection aperture size or by the camera modulation transfer function (MTF), it is the atmosphere that usually limits image quality for long range imaging. The main atmospheric distortions are caused by optical turbulence, absorption, and scattering by particulates in the atmosphere. The effects of the turbulent medium over long/short exposures are image blur and wavefront tilts that cause spatio-temporal image shifts. This blur limits the frequency of line pairs that can be resolved in the target's image and thus affects the ability to acquire targets. The observer appears to be able to ignore large-scale distortions while small-scale distortions blur the image and degrade resolution. Resolution degradations due to turbulence are included in current performance models by the use of an atmospheric MTF. Turbulence distortion effects are characterized by both short and long exposure MTFs. In addition to turbulence, scattering and absorption produced by molecules and aerosols in the atmosphere cause both attenuation and additional image blur according to the atmospheric aerosol MTF. The absorption can have significant effect on target acquisition in infrared (IR) imaging. In the present work, a brief overview and discussion of atmospheric effects on target acquisition in the IR is given.

  19. The ALICE data acquisition system

    NASA Astrophysics Data System (ADS)

    Carena, F.; Carena, W.; Chapeland, S.; Chibante Barroso, V.; Costa, F.; Dénes, E.; Divià, R.; Fuchs, U.; Grigore, A.; Kiss, T.; Simonetti, G.; Soós, C.; Telesca, A.; Vande Vyvre, P.; von Haller, B.

    2014-03-01

    In this paper we describe the design, the construction, the commissioning and the operation of the Data Acquisition (DAQ) and Experiment Control Systems (ECS) of the ALICE experiment at the CERN Large Hadron Collider (LHC). The DAQ and the ECS are the systems used respectively for the acquisition of all physics data and for the overall control of the experiment. They are two computing systems made of hundreds of PCs and data storage units interconnected via two networks. The collection of experimental data from the detectors is performed by several hundreds of high-speed optical links. We describe in detail the design considerations for these systems handling the extreme data throughput resulting from central lead ions collisions at LHC energy. The implementation of the resulting requirements into hardware (custom optical links and commercial computing equipment), infrastructure (racks, cooling, power distribution, control room), and software led to many innovative solutions which are described together with a presentation of all the major components of the systems, as currently realized. We also report on the performance achieved during the first period of data taking (from 2009 to 2013) often exceeding those specified in the DAQ Technical Design Report.

  20. 48 CFR 908.7101-3 - Direct acquisition.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Direct acquisition. 908.7101-3 Section 908.7101-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY COMPETITION ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES AND SERVICES Acquisition of Special Items 908.7101-3 Direct acquisition. Vehicles may be acquired by...

  1. 48 CFR 218.170 - Additional acquisition flexibilities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Additional acquisition flexibilities. 218.170 Section 218.170 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES EMERGENCY ACQUISITIONS Available Acquisition Flexibilities...

  2. Symbiont acquisition as neoseme: origin of species and higher taxa

    NASA Technical Reports Server (NTRS)

    Bermudes, D.; Margulis, L.

    1987-01-01

    We examine the hypothesis that, in the origin of species and higher taxa of eukaryotes, symbiont acquisition followed by partner integration has been equivalent to neoseme appearance leading to speciation. The formation of stable symbiotic associations involves partner-surface recognition, behavioral and metabolic interaction, and, in some cases, gene product (RNA, protein) and genic (RNA, DNA) integration. This analysis is applied here to examples of neosemes that define specific taxa and to neosemes in plants, fungi, and animals that involve the appearance of new types of tissue. If this hypothesis is correct--if the origin of major genetic variation leading to speciation and even higher taxa may occur through symbiont acquisition and integration--then the analysis of "origins of species and higher taxa" becomes analogous to the study of microbial community ecology.

  3. Outer membrane protein purification.

    PubMed

    Arigita, C; Jiskoot, W; Graaf, M R; Kersten, G F

    2001-01-01

    The major outer membrane proteins (OMPs) from Neisseria meningitidis, which are expressed at high levels, are subdivided in five classes based on molecular weight (1,2) (see Table 1). Table 1 Major Meningococcal Outer-Membrane Proteins Outer-membrane proteins Name Molecular maass Function/characteristics Class 1 PorA 44-47 kDa Porin Class 2/3 PorB 37-42 kDa Porin Class 4 Rmp Reductionmodifiableprotein, unknown Class 5 Opa 26-30 kDa Adhesion,opacity protein Opc 25 kDa Invasion, opacity protein Iron-regulated proteins Mirp 37 kDa Iron acquisition (?);majoriron-regulatedprotein FrpB 70 kDa Ferric enterobactin receptor (also FetA) Adapted from ref. (1). PMID:21336748

  4. Learning the manifold of quality ultrasound acquisition.

    PubMed

    El-Zehiry, Noha; Yan, Michelle; Good, Sara; Fang, Tong; Zhou, S Kevin; Grady, Leo

    2013-01-01

    Ultrasound acquisition is a challenging task that requires simultaneous adjustment of several acquisition parameters (the depth, the focus, the frequency and its operation mode). If the acquisition parameters are not properly chosen, the resulting image will have a poor quality and will degrade the patient diagnosis and treatment workflow. Several hardware-based systems for autotuning the acquisition parameters have been previously proposed, but these solutions were largely abandoned because they failed to properly account for tissue inhomogeneity and other patient-specific characteristics. Consequently, in routine practice the clinician either uses population-based parameter presets or manually adjusts the acquisition parameters for each patient during the scan. In this paper, we revisit the problem of autotuning the acquisition parameters by taking a completely novel approach and producing a solution based on image analytics. Our solution is inspired by the autofocus capability of conventional digital cameras, but is significantly more challenging because the number of acquisition parameters is large and the determination of "good quality" images is more difficult to assess. Surprisingly, we show that the set of acquisition parameters which produce images that are favored by clinicians comprise a 1D manifold, allowing for a real-time optimization to maximize image quality. We demonstrate our method for acquisition parameter autotuning on several live patients, showing that our system can start with a poor initial set of parameters and automatically optimize the parameters to produce high quality images. PMID:24505657

  5. Galileo probe relay receiver - Acquisition and tracking

    NASA Technical Reports Server (NTRS)

    Von Der Embse, U. A.

    1983-01-01

    The probe-to-orbiter data link for the Jovian mission uses a Manchester encoded BPSK waveform which is demodulated by the Galileo probe relay receiver. Signal acquisition and tracking consists of a sequential probability ratio signal search, frequency acquisition with a least-squares estimator, wide-band phase lock acquisition, and a self-regulating mode control. A discrete Fourier transform serves as the basic mechanism to generate the algorithms that provide this orderly transition to phase tracking. Acquisition and tracking is addressed in this paper with emphasis on key algorithms, rationale, and theoretical/measured performance.

  6. The myth of data acquisition rate.

    PubMed

    Felinger, Attila; Kilár, Anikó; Boros, Borbála

    2015-01-01

    With the need for high-frequency data acquisition, the influence of the data acquisition rate on the quality of the digitized signal is often discussed and also misinterpreted. In this study we show that undersampling of the signal, i.e. low data acquisition rate will not cause band broadening. Users of modern instrumentation and authors are frequently misled by hidden features of the data handling software they use. Very often users are unaware of the noise filtering algorithms that run parallel with data acquisition and that lack of information misleads them. We also demonstrate that undersampled signals can be restored by a proper trigonometric interpolation. PMID:25479882

  7. The Channel Acquisition Slot Protocol

    NASA Astrophysics Data System (ADS)

    Milian, Arturo Valencia; Tamura, Shinsuke; Yanase, Tatsuro

    This paper discusses the Channel Acquisition Slot Protocol (CASP), a new protocol for the Media Access Control (MAC) of wireless networks. The CASP is intended to provide a more efficient use of communication channels; this is achieved by mechanisms for eliminating contentions among network stations and by an implicit handling of acknowledge messages. To avoid collisions of messages, the protocol divides the time into short slots, each of which is assigned to a single station, and any station must wait its own slot to start its transmission. However once a transmission is started the slot can be extended and no other station can make use of the channel. The use of an implicit acknowledge mechanism removes the necessity of an acknowledge message exchange for every transmission. In various simulated scenarios the CASP shows a 25% better throughput efficiency compared with the one of the IEEE802.11 protocol.

  8. Surface acquisition through virtual milling

    NASA Technical Reports Server (NTRS)

    Merriam, Marshal L.

    1993-01-01

    Surface acquisition deals with the reconstruction of three dimensional objects from a set of data points. The most straightforward techniques require human intervention, a time consuming proposition. It is desirable to develop a fully automated alternative. Such a method is proposed in this paper. It makes use of surface measurements obtained from a 3-D laser digitizer - an instrument which provides the (x,y,z) coordinates of surface data points from various viewpoints. These points are assembled into several partial surfaces using a visibility constraint and a 2-D triangulation technique. Reconstruction of the final object requires merging these partial surfaces. This is accomplished through a procedure that emulates milling, a standard machining operation. From a geometrical standpoint the problem reduces to constructing the intersection of two or more non-convex polyhedra.

  9. Passive acquisition of CLIPS rules

    NASA Technical Reports Server (NTRS)

    Kovarik, Vincent J., Jr.

    1991-01-01

    The automated acquisition of knowledge by machine has not lived up to expectations, and knowledge engineering remains a human intensive task. Part of the reason for the lack of success is the difference in the cognitive focus of the expert. The expert must shift his or her focus from the subject domain to that of the representation environment. In doing so this cognitive shift introduces opportunity for errors and omissions. Presented here is work that observes the expert interact with a simulation of the domain. The system logs changes in the simulation objects and the expert's actions in response to those changes. This is followed by the application of inductive reasoning to move the domain specific rules observed to general domain rules.

  10. Genital Inflammation and the Risk of HIV Acquisition in Women

    PubMed Central

    Masson, Lindi; Passmore, Jo-Ann S.; Liebenberg, Lenine J.; Werner, Lise; Baxter, Cheryl; Arnold, Kelly B.; Williamson, Carolyn; Little, Francesca; Mansoor, Leila E.; Naranbhai, Vivek; Lauffenburger, Douglas A.; Ronacher, Katharina; Walzl, Gerhard; Garrett, Nigel J.; Williams, Brent L.; Couto-Rodriguez, Mara; Hornig, Mady; Lipkin, W. Ian; Grobler, Anneke; Abdool Karim, Quarraisha; Abdool Karim, Salim S.

    2015-01-01

    Background. Women in Africa, especially young women, have very high human immunodeficiency virus (HIV) incidence rates that cannot be fully explained by behavioral risks. We investigated whether genital inflammation influenced HIV acquisition in this group. Methods. Twelve selected cytokines, including 9 inflammatory cytokines and chemokines (interleukin [IL]-1α, IL-1β, IL-6, tumor necrosis factor-α, IL-8, interferon-γ inducible protein-10 [IP-10], monocyte chemoattractant protein-1, macrophage inflammatory protein [MIP]-1α, MIP-1β), hematopoietic IL-7, and granulocyte macrophage colony-stimulating factor, and regulatory IL-10 were measured prior to HIV infection in cervicovaginal lavages from 58 HIV seroconverters and 58 matched uninfected controls and in plasma from a subset of 107 of these women from the Centre for the AIDS Programme of Research in South Africa 004 tenofovir gel trial. Results. HIV seroconversion was associated with raised genital inflammatory cytokines (including chemokines MIP-1α, MIP-1β, and IP-10). The risk of HIV acquisition was significantly higher in women with evidence of genital inflammation, defined by at least 5 of 9 inflammatory cytokines being raised (odds ratio, 3.2; 95% confidence interval, 1.3–7.9; P = .014). Genital cytokine concentrations were persistently raised (for about 1 year before infection), with no readily identifiable cause despite extensive investigation of several potential factors, including sexually transmitted infections and systemic cytokines. Conclusions. Elevated genital concentrations of HIV target cell–recruiting chemokines and a genital inflammatory profile contributes to the high risk of HIV acquisition in these African women. PMID:25900168

  11. 76 FR 7685 - (General Provisions) Contract Appeals and the Acquisition Regulation: General, Acquisition...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-11

    ...The Department of Energy (DOE) is amending the Department of Energy Acquisition Regulation (DEAR) regulations on Acquisition Planning, and Contracting Methods and Contract Types to make changes to conform to the Federal Acquisition Regulation (FAR), remove out-of-date coverage, and update references. Today's rule does not alter substantive rights or obligations under current...

  12. Enseignement des langues et theories d'acquisition Sprachunterricht unde Spracherwerbstheorien. (Language Teaching and Acquisition Theories).

    ERIC Educational Resources Information Center

    Schwarz, Alexander, Ed.

    1996-01-01

    This collection of articles on language teaching and language acquisition theories includes: "Enseignement des langues et theories de l'acquisition. Introduction au colloque" ("Language Teaching and Theories of Acquisition. Introduction to the Colloquium") (Rene Richterich); "Apprendre une langue dans l'interaction verbale" ("Language Learning in…

  13. Are Language-Acquisition Strategies Universal?

    ERIC Educational Resources Information Center

    Akiyama, Michael M.

    1984-01-01

    Tests the universality hypothesis of language acquisition by asking young monolingual English and Japanese children to verify true affirmatives, false affirmatives, false negatives, and true negatives. The hypothesis was not supported in the case of Japanese-speaking children. A theory of cross-linguistic language acquisition is proposed.…

  14. 77 FR 59339 - Acquisition of Commercial Items

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-27

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 212 Acquisition of Commercial Items CFR Correction 212.504 In Title 48 of the Code of Federal Regulations, Chapter 2 (Parts 201--299), revised as of...

  15. Second Language Acquisition and Language Teaching

    ERIC Educational Resources Information Center

    Tragant, Elsa; Muñoz, Carmen

    2004-01-01

    After discussing the ties between language teaching and second language acquisition research, the present paper reviews the role that second language acquisition research has played on two recent pedagogical proposals. First, communicative language teaching, advocated in the early eighties, in which focus on the code was excluded, and then the…

  16. Teacher Trainees' Information Acquisition in Lesson Planning

    ERIC Educational Resources Information Center

    Tanni, Mikko

    2012-01-01

    Introduction: Few studies have addressed teacher trainees' information acquisition in the context of a task. The paper reports findings from an empirical study on teacher trainees' use of information (seeking) channels and sources, and their modes of information acquisition in lesson planning. Method: Semi-structured (retrospectively held)…

  17. You Can Accurately Predict Land Acquisition Costs.

    ERIC Educational Resources Information Center

    Garrigan, Richard

    1967-01-01

    Land acquisition costs were tested for predictability based upon the 1962 assessed valuations of privately held land acquired for campus expansion by the University of Wisconsin from 1963-1965. By correlating the land acquisition costs of 108 properties acquired during the 3 year period with--(1) the assessed value of the land, (2) the assessed…

  18. 48 CFR 970.2301 - Sustainable acquisition.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition....

  19. 48 CFR 23.103 - Sustainable acquisitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Sustainable Acquisition Policy 23.103 Sustainable acquisitions. (a... the products are— (1) Energy-efficient (ENERGY STAR ® or Federal Energy Management Program...

  20. 48 CFR 970.2301 - Sustainable acquisition.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition....

  1. 48 CFR 970.2301 - Sustainable acquisition.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition....

  2. 78 FR 45168 - Acquisition Regulations: Export Control

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-26

    ... of Energy (DOE) published a proposed rulemaking in the Federal Register (78 FR 35195) to amend the..., Office of Acquisition and Project Management, MA-611, 1000 Independence Avenue SW., Washington, DC 20585..., Office of Acquisition and Project Management, Department of Energy. BILLING CODE 6450-01-P...

  3. 48 CFR 970.2301 - Sustainable acquisition.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    .... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable acquisition....

  4. Integration and Reuse in Cognitive Skill Acquisition

    ERIC Educational Resources Information Center

    Salvucci, Dario D.

    2013-01-01

    Previous accounts of cognitive skill acquisition have demonstrated how procedural knowledge can be obtained and transformed over time into skilled task performance. This article focuses on a complementary aspect of skill acquisition, namely the integration and reuse of previously known component skills. The article posits that, in addition to…

  5. The Acquisition of Causal Connectives in Turkish.

    ERIC Educational Resources Information Center

    Aksu, Ayhan

    The elicited speech of 26 Turkish children ranging in age from 2;0 to 4;6 was examined with respect to causality. The developmental sequence of the acquisition of causal connectives showed a progression from the use of no explicit connectives to the acquisition of connectives that are context-dependent. The next stage in this progression was the…

  6. New Dimensions in Second Language Acquisition Research.

    ERIC Educational Resources Information Center

    Andersen, Roger W., Ed.

    The following papers are included: (1) "Some Common Goals for Second and First Language Acquisition Research" by Kenji Hakuta; (2) "Research on the Measurement of Affective Variables: Some Remaining Questions" by John W. Oller, Jr.; (3) "The Effects of Neurological Age on Nonprimary Language Acquisition" by Thomas Scovel; (4) "Exceptions to…

  7. Acquisitions Allocations: Fairness, Equity and Bundled Pricing.

    ERIC Educational Resources Information Center

    Packer, Donna

    2001-01-01

    Examined the effect of an interdisciplinary Web-based citation database with full text, the ProQuest Research Library, on the Western State University library's acquisitions allocation plan. Used list price of full-text journals to calculate increases in acquisitions funding. A list of articles discussing formula allocation is appended.…

  8. 40 CFR 35.2210 - Land acquisition.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Administrator has determined that applicable provisions of 40 CFR part 4 have been met. ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Land acquisition. 35.2210 Section 35... STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works § 35.2210 Land acquisition....

  9. 76 FR 76899 - Federal Acquisition Regulation; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-09

    ... FR 68039). The final rule amended the Federal Acquisition Regulation (FAR) to revise the definitions..., 2011 (76 FR 68039). The rule amended the Federal Acquisition Regulation (FAR) by revising the... that comprised the Netherlands Antilles. ] DATES: Effective Date: December 9, 2011. FOR...

  10. 48 CFR 234.004 - Acquisition strategy.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... (1) See 209.570 for policy applicable to acquisition strategies that consider the use of lead system... acquisition strategy. The documentation— (1) Shall include an explanation of the level of program risk; and (2) If program risk is determined to be high, shall outline the steps taken to reduce program risk...

  11. Acquisition of Noun Incorporation in Inuktitut.

    ERIC Educational Resources Information Center

    Allen, Shanley; Crago, Martha

    An investigation of the first language acquisition of productive nouns in Inuktitut (Inupiaq) is presented. This study begins with descriptions of noun incorporation, relevant aspects of the structure of Inuktitut, and working criteria of productivity. Acquisition data from Inuktitut and corroborating data from Greenlandic are outlined and…

  12. The PAN-DA data acquisition system

    SciTech Connect

    Petravick, D.; Berg, D.; Berman, E.; Bernett, M.; Constanta-Fanourakis, P.; Dorries, T.; Haire, M.; Kaczar, K; MacKinnon, B.; Moore, C.; Nicinski, T.; Oleynik, G.; Pordes, R.; Sergey, G.; Votava, M.; White, V.

    1989-05-01

    The Online and Data Acquisition software groups at Fermi National Accelerator Laboratory have extended the VAXONLINE data acquisition package to include a VME based data path. The resulting environment, PAN-DA, provides a high throughput for logging, filtering, formatting and selecting events. 10 refs., 1 fig.

  13. 75 FR 69009 - Acquisition Regulation: Socioeconomic Programs

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-10

    ...The Department of Energy (DOE) is amending the Department of Energy Acquisition Regulation (DEAR) Socioeconomic Programs to make changes to conform to the Federal Acquisition Regulation (FAR), remove out-of-date coverage, and update references. Today's rule does not alter substantive rights or obligations under current...

  14. 76 FR 15901 - Acquisition Regulation Miscellaneous Changes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    .... SUMMARY: The Department of the Interior (DOI) is proposing to revise the Department of the Interior Acquisition Regulation (DIAR) in order to make minor corrections to DOI acquisition procedures to be... e-mail tiffany_schermerhorn@ios.doi.gov . SUPPLEMENTARY INFORMATION: I. Background This rule...

  15. 75 FR 19827 - Acquisition Regulation Rewrite

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ...The Department of the Interior (DOI) is taking interim final action on administrative changes to the Department of the Interior Acquisition Regulation (DIAR). This action revises the DIAR, 48 CFR Chapter 14, but does not impose any new requirements on DOI contractors. The revisions in this interim final rule will make minor corrections to and streamline DOI acquisition processes to be......

  16. Theories and Research on Second Language Acquisition.

    ERIC Educational Resources Information Center

    Cornejo, Ricardo J.; Cornejo, Luz O.

    Intended for students, teachers, parents, and administrators in charge of educating bilingual children, the monograph provides a summarized account of the information available regarding theories and research in second language acquisition, divided into four chapters. Chapter I is an historical overview of language acquisition theories,…

  17. The Chateau de Cristal data acquisition system

    SciTech Connect

    Villard, M.M.

    1987-08-01

    This data acquisition system is built on several dedicated data transfer buses: ADC data readout through the FERA bus, parallel data processing in two VME crates. High data rates and selectivities are performed via this acquisition structure and new developed processing units. The system modularity allows various experiments with additional detectors.

  18. 12 CFR 238.13 - Prohibited acquisitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Prohibited acquisitions. 238.13 Section 238.13 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM (CONTINUED) SAVINGS AND LOAN HOLDING COMPANIES (REGULATION LL) Acquisitions of Saving Association...

  19. 12 CFR 238.13 - Prohibited acquisitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 4 2013-01-01 2013-01-01 false Prohibited acquisitions. 238.13 Section 238.13 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM (CONTINUED) SAVINGS AND LOAN HOLDING COMPANIES (REGULATION LL) Acquisitions of Saving Association...

  20. 12 CFR 238.13 - Prohibited acquisitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 4 2012-01-01 2012-01-01 false Prohibited acquisitions. 238.13 Section 238.13 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM (CONTINUED) SAVINGS AND LOAN HOLDING COMPANIES (REGULATION LL) Acquisitions of Saving Association...

  1. Neural Plasticity in Speech Acquisition and Learning

    ERIC Educational Resources Information Center

    Zhang, Yang; Wang, Yue

    2007-01-01

    Neural plasticity in speech acquisition and learning is concerned with the timeline trajectory and the mechanisms of experience-driven changes in the neural circuits that support or disrupt linguistic function. In this selective review, we discuss the role of phonetic learning in language acquisition, the "critical period" of learning, the agents…

  2. Integration and reuse in cognitive skill acquisition.

    PubMed

    Salvucci, Dario D

    2013-07-01

    Previous accounts of cognitive skill acquisition have demonstrated how procedural knowledge can be obtained and transformed over time into skilled task performance. This article focuses on a complementary aspect of skill acquisition, namely the integration and reuse of previously known component skills. The article posits that, in addition to mechanisms that proceduralize knowledge into more efficient forms, skill acquisition requires tight integration of newly acquired knowledge and previously learned knowledge. Skill acquisition also benefits from reuse of existing knowledge across disparate task domains, relying on indexicals to reference and share necessary information across knowledge components. To demonstrate these ideas, the article proposes a computational model of skill acquisition from instructions focused on integration and reuse, and applies this model to account for behavior across seven task domains. PMID:23551386

  3. CEBAF Distributed Data Acquisition System

    SciTech Connect

    Trent Allison; Thomas Powers

    2005-05-01

    There are thousands of signals distributed throughout Jefferson Lab's Continuous Electron Beam Accelerator Facility (CEBAF) that are useful for troubleshooting and identifying instabilities. Many of these signals are only available locally or monitored by systems with small bandwidths that cannot identify fast transients. The Distributed Data Acquisition (Dist DAQ) system will sample and record these signals simultaneously at rates up to 40 Msps. Its primary function will be to provide waveform records from signals throughout CEBAF to the Experimental Physics and Industrial Control System (EPICS). The waveforms will be collected after the occurrence of an event trigger. These triggers will be derived from signals such as periodic timers or accelerator faults. The waveform data can then be processed to quickly identify beam transport issues, thus reducing down time and increasing CEBAF performance. The Dist DAQ system will be comprised of multiple standalone chassis distributed throughout CEBAF. They will be interconnected via a fiber optic network to facilitate the global triggering of events. All of the chassis will also be connected directly to the CEBAF Ethernet and run EPICS locally. This allows for more flexibility than the typical configuration of a single board computer and other custom printed circuit boards (PCB) installed in a card cage.

  4. Multiple channel data acquisition system

    DOEpatents

    Crawley, H. Bert; Rosenberg, Eli I.; Meyer, W. Thomas; Gorbics, Mark S.; Thomas, William D.; McKay, Roy L.; Homer, Jr., John F.

    1990-05-22

    A multiple channel data acquisition system for the transfer of large amounts of data from a multiplicity of data channels has a plurality of modules which operate in parallel to convert analog signals to digital data and transfer that data to a communications host via a FASTBUS. Each module has a plurality of submodules which include a front end buffer (FEB) connected to input circuitry having an analog to digital converter with cache memory for each of a plurality of channels. The submodules are interfaced with the FASTBUS via a FASTBUS coupler which controls a module bus and a module memory. The system is triggered to effect rapid parallel data samplings which are stored to the cache memories. The cache memories are uploaded to the FEBs during which zero suppression occurs. The data in the FEBs is reformatted and compressed by a local processor during transfer to the module memory. The FASTBUS coupler is used by the communications host to upload the compressed and formatted data from the module memory. The local processor executes programs which are downloaded to the module memory through the FASTBUS coupler.

  5. Multiple channel data acquisition system

    DOEpatents

    Crawley, H.B.; Rosenberg, E.I.; Meyer, W.T.; Gorbics, M.S.; Thomas, W.D.; McKay, R.L.; Homer, J.F. Jr.

    1990-05-22

    A multiple channel data acquisition system for the transfer of large amounts of data from a multiplicity of data channels has a plurality of modules which operate in parallel to convert analog signals to digital data and transfer that data to a communications host via a FASTBUS. Each module has a plurality of submodules which include a front end buffer (FEB) connected to input circuitry having an analog to digital converter with cache memory for each of a plurality of channels. The submodules are interfaced with the FASTBUS via a FASTBUS coupler which controls a module bus and a module memory. The system is triggered to effect rapid parallel data samplings which are stored to the cache memories. The cache memories are uploaded to the FEBs during which zero suppression occurs. The data in the FEBs is reformatted and compressed by a local processor during transfer to the module memory. The FASTBUS coupler is used by the communications host to upload the compressed and formatted data from the module memory. The local processor executes programs which are downloaded to the module memory through the FASTBUS coupler. 25 figs.

  6. VLBI data, acquisition, environmental effects

    NASA Technical Reports Server (NTRS)

    Herring, Thomas A.

    1995-01-01

    During this quadrennium, very long baseline interferometry (VLBI) data acquisition and system development has focused on improving the accuracy of the system sufficiently to allow the determination of reliable estimates of height variations. Associated with this aim has been improvements in the determinations of horizontal velocity fields, monitoring water vapor delay using interferometric methods, and improvements to Earth rotation measurements. The primary aims of the improvements to height measurement accuracy have been to directly measure the contemporary magnitudes of post glacial rebound, and to determine a height reference system for measuring global sea level rise. High frequency Earth rotation studies have been carried out to better define the transformation parameters from an inertial coordinate system to an Earth fixed one, and to better understand the coupling between the components of the atmosphere-ocean-solid Earth system. Two major VLBI campaigns were carried out in support of these studies: (1) Epoch-92 in July 1992 and (2) Cont-94 in January 1994. Each of these campaigns lasted approximately two weeks and involved multiple VLBI networks operating simultaneously in addition to other space geodetic systems operating during these periods. Two major compilations of the VLBI results (and results from other space geodetic systems) have been published during this quadrennium.

  7. Psychological tools for knowledge acquisition

    NASA Technical Reports Server (NTRS)

    Rueter, Henry H.; Olson, Judith Reitman

    1988-01-01

    Knowledge acquisition is said to be the biggest bottleneck in the development of expert systems. The problem is getting the knowledge out of the expert's head and into a computer. In cognitive psychology, characterizing metal structures and why experts are good at what they do is an important research area. Is there some way that the tools that psychologists have developed to uncover mental structure can be used to benefit knowledge engineers? We think that the way to find out is to browse through the psychologist's toolbox to see what there is in it that might be of use to knowledge engineers. Expert system developers have relied on two standard methods for extracting knowledge from the expert: (1) the knowledge engineer engages in an intense bout of interviews with the expert or experts, or (2) the knowledge engineer becomes an expert himself, relying on introspection to uncover the basis of his own expertise. Unfortunately, these techniques have the difficulty that often the expert himself isn't consciously aware of the basis of his expertise. If the expert himself isn't conscious of how he solves problems, introspection is useless. Cognitive psychology has faced similar problems for many years and has developed exploratory methods that can be used to discover cognitive structure from simple data.

  8. Colony image acquisition and segmentation

    NASA Astrophysics Data System (ADS)

    Wang, W. X.

    2007-12-01

    For counting of both colonies and plaques, there is a large number of applications including food, dairy, beverages, hygiene, environmental monitoring, water, toxicology, sterility testing, AMES testing, pharmaceuticals, paints, sterile fluids and fungal contamination. Recently, many researchers and developers have made efforts for this kind of systems. By investigation, some existing systems have some problems. The main problems are image acquisition and image segmentation. In order to acquire colony images with good quality, an illumination box was constructed as: the box includes front lightning and back lightning, which can be selected by users based on properties of colony dishes. With the illumination box, lightning can be uniform; colony dish can be put in the same place every time, which make image processing easy. The developed colony image segmentation algorithm consists of the sub-algorithms: (1) image classification; (2) image processing; and (3) colony delineation. The colony delineation algorithm main contain: the procedures based on grey level similarity, on boundary tracing, on shape information and colony excluding. In addition, a number of algorithms are developed for colony analysis. The system has been tested and satisfactory.

  9. 48 CFR 19.502 - Setting aside acquisitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Setting aside acquisitions. 19.502 Section 19.502 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 19.502 Setting aside acquisitions....

  10. 48 CFR 1.102-3 - Acquisition team.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisition team. 1.102-3... ACQUISITION REGULATIONS SYSTEM Purpose, Authority, Issuance 1.102-3 Acquisition team. The purpose of defining the Federal Acquisition Team (Team) in the Guiding Principles is to ensure that participants in...

  11. 48 CFR 1401.7001-4 - Acquisition performance measurement systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Acquisition performance measurement systems. 1401.7001-4 Section 1401.7001-4 Federal Acquisition Regulations System DEPARTMENT OF THE... Acquisition performance measurement systems. (a) The acquisition performance measurement system is a...

  12. 48 CFR 1401.7001-4 - Acquisition performance measurement systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Acquisition performance measurement systems. 1401.7001-4 Section 1401.7001-4 Federal Acquisition Regulations System DEPARTMENT OF THE... Acquisition performance measurement systems. (a) The acquisition performance measurement system is a...

  13. 48 CFR 1401.7001-4 - Acquisition performance measurement systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Acquisition performance measurement systems. 1401.7001-4 Section 1401.7001-4 Federal Acquisition Regulations System DEPARTMENT OF THE... Acquisition performance measurement systems. (a) The acquisition performance measurement system is a...

  14. 48 CFR 252.211-7000 - Acquisition streamlining.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Acquisition streamlining. 252.211-7000 Section 252.211-7000 Federal Acquisition Regulations System DEFENSE ACQUISITION... of Provisions And Clauses 252.211-7000 Acquisition streamlining. As prescribed in 211.002-70, use...

  15. 48 CFR 252.211-7000 - Acquisition streamlining.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Acquisition streamlining. 252.211-7000 Section 252.211-7000 Federal Acquisition Regulations System DEFENSE ACQUISITION... of Provisions And Clauses 252.211-7000 Acquisition streamlining. As prescribed in 211.002-70, use...

  16. 48 CFR 27.406 - Acquisition of data.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acquisition of data. 27.406 Section 27.406 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS PATENTS, DATA, AND COPYRIGHTS Rights in Data and Copyrights 27.406 Acquisition...

  17. 48 CFR 27.406-3 - Major system acquisition.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Major system acquisition. 27.406-3 Section 27.406-3 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Major system acquisition. (a) The clause at 52.227-21, Technical Data Declaration, Revision,...

  18. 48 CFR 5.405 - Exchange of acquisition information.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Exchange of acquisition information. 5.405 Section 5.405 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING PUBLICIZING CONTRACT ACTIONS Release of Information 5.405 Exchange of...

  19. 48 CFR 27.406 - Acquisition of data.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Acquisition of data. 27.406 Section 27.406 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS PATENTS, DATA, AND COPYRIGHTS Rights in Data and Copyrights 27.406 Acquisition...

  20. How do oncoprotein mutations rewire protein-protein interaction networks?

    PubMed

    Bowler, Emily H; Wang, Zhenghe; Ewing, Rob M

    2015-01-01

    The acquisition of mutations that activate oncogenes or inactivate tumor suppressors is a primary feature of most cancers. Mutations that directly alter protein sequence and structure drive the development of tumors through aberrant expression and modification of proteins, in many cases directly impacting components of signal transduction pathways and cellular architecture. Cancer-associated mutations may have direct or indirect effects on proteins and their interactions and while the effects of mutations on signaling pathways have been widely studied, how mutations alter underlying protein-protein interaction networks is much less well understood. Systematic mapping of oncoprotein protein interactions using proteomics techniques as well as computational network analyses is revealing how oncoprotein mutations perturb protein-protein interaction networks and drive the cancer phenotype. PMID:26325016

  1. MS1 Peptide Ion Intensity Chromatograms in MS2 (SWATH) Data Independent Acquisitions. Improving Post Acquisition Analysis of Proteomic Experiments*

    PubMed Central

    Rardin, Matthew J.; Schilling, Birgit; Cheng, Lin-Yang; MacLean, Brendan X.; Sorensen, Dylan J.; Sahu, Alexandria K.; MacCoss, Michael J.; Vitek, Olga; Gibson, Bradford W.

    2015-01-01

    Quantitative analysis of discovery-based proteomic workflows now relies on high-throughput large-scale methods for identification and quantitation of proteins and post-translational modifications. Advancements in label-free quantitative techniques, using either data-dependent or data-independent mass spectrometric acquisitions, have coincided with improved instrumentation featuring greater precision, increased mass accuracy, and faster scan speeds. We recently reported on a new quantitative method called MS1 Filtering (Schilling et al. (2012) Mol. Cell. Proteomics 11, 202–214) for processing data-independent MS1 ion intensity chromatograms from peptide analytes using the Skyline software platform. In contrast, data-independent acquisitions from MS2 scans, or SWATH, can quantify all fragment ion intensities when reference spectra are available. As each SWATH acquisition cycle typically contains an MS1 scan, these two independent label-free quantitative approaches can be acquired in a single experiment. Here, we have expanded the capability of Skyline to extract both MS1 and MS2 ion intensity chromatograms from a single SWATH data-independent acquisition in an Integrated Dual Scan Analysis approach. The performance of both MS1 and MS2 data was examined in simple and complex samples using standard concentration curves. Cases of interferences in MS1 and MS2 ion intensity data were assessed, as were the differentiation and quantitation of phosphopeptide isomers in MS2 scan data. In addition, we demonstrated an approach for optimization of SWATH m/z window sizes to reduce interferences using MS1 scans as a guide. Finally, a correlation analysis was performed on both MS1 and MS2 ion intensity data obtained from SWATH acquisitions on a complex mixture using a linear model that automatically removes signals containing interferences. This work demonstrates the practical advantages of properly acquiring and processing MS1 precursor data in addition to MS2 fragment ion

  2. 48 CFR 12.301 - Solicitation provisions and contract clauses for the acquisition of commercial items.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and contract clauses for the acquisition of commercial items. 12.301 Section 12.301 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING ACQUISITION OF COMMERCIAL ITEMS Solicitation Provisions and Contract Clauses for the Acquisition of Commercial Items...

  3. Electronic Flash In Data Acquisition

    NASA Astrophysics Data System (ADS)

    Miller, C. E.

    1982-02-01

    Photographic acquisition of data often may be simplified, or the data quality improved upon by employing electronic flash sources with traditional equipment or techniques. The relatively short flash duration compared to movie camera shutters, or to the long integration time of video camera provides improved spatial resolution through blur reduction, particularly important as image movement becomes a significant fraction of film format dimension. Greater accuracy typically is achieved in velocity and acceleration determinations by using a stroboscopic light source rather than a movie camera frame-rate control as a time standard. Electrical efficiency often is an important advantage of electronic flash sources since almost any necessary light level for exposure may be produced, yet the source typically is "off" most of the time. Various synchronization techniques greatly expand the precise control of exposure. Biomechanical and sports equipment studies may involve velocities up to 200 feet-per-second, and often will have associated very rapid actions of interest. The need for brief exposures increases H.s one "ZOOMS in on the action." In golf, for example, the swing may be examined using 100 microsecond (Us) flashes at rates of 60 or 120 flashes-per-second (FPS). Accurate determination of linear and rotational velocity of the ball requires 10 Us flashes at 500-1,000 FPS, while sub-Us flashes at 20,000-50,000 FPS are required to resolve the interaction of the ball and the club, head. Some seldom. used techniques involving streak photography are described, with enhanced results obtained by combining strobe with the usual continuous light source. The combination of strobe and a fast electro-mechanical shutter is considered for Us photography under daylight conditions.

  4. A data acquisition architecture for the SSC

    SciTech Connect

    Partridge, R.

    1990-01-01

    An SSC data acquisition architecture applicable to high-p{sub T} detectors is described. The architecture is based upon a small set of design principles that were chosen to simplify communication between data acquisition elements while providing the required level of flexibility and performance. The architecture features an integrated system for data collection, event building, and communication with a large processing farm. The interface to the front end electronics system is also discussed. A set of design parameters is given for a data acquisition system that should meet the needs of high-p{sub T} detectors at the SSC.

  5. TFTR diagnostic control and data acquisition system

    NASA Astrophysics Data System (ADS)

    Sauthoff, N. R.; Daniels, R. E.

    1985-05-01

    General computerized control and data-handling support for TFTR diagnostics is presented within the context of the Central Instrumentation, Control and Data Acquisition (CICADA) System. Procedures, hardware, the interactive man-machine interface, event-driven task scheduling, system-wide arming and data acquisition, and a hierarchical data base of raw data and results are described. Similarities in data structures involved in control, monitoring, and data acquisition afford a simplification of the system functions, based on ``groups'' of devices. Emphases and optimizations appropriate for fusion diagnostic system designs are provided. An off-line data reduction computer system is under development.

  6. TFTR diagnostic control and data acquisition system

    SciTech Connect

    Sauthoff, N.R.; Daniels, R.E.; PPL Computer Division

    1985-05-01

    General computerized control and data-handling support for TFTR diagnostics is presented within the context of the Central Instrumentation, Control and Data Acquisition (CICADA) System. Procedures, hardware, the interactive man--machine interface, event-driven task scheduling, system-wide arming and data acquisition, and a hierarchical data base of raw data and results are described. Similarities in data structures involved in control, monitoring, and data acquisition afford a simplification of the system functions, based on ''groups'' of devices. Emphases and optimizations appropriate for fusion diagnostic system designs are provided. An off-line data reduction computer system is under development.

  7. Evaluation of Resource Acquisition Approaches : Final Report.

    SciTech Connect

    O`Neill, Maura L.; Mortimer, Tom; Palermini, Debbi; Nelson, Kari

    1991-09-12

    Over the last few years, Bonneville has been addressing this need and has developed numerous ways of acquiring resources. Four of these Approaches, the Competitive Acquisition, Billing Credits, and Targeted Acquisition Programs, and the Cowlitz Falls Hydroelectric Project, were the subject of this evaluation project. Each Approach is currently in different stages of a process, and Bonneville felt it was an appropriate time that an evaluation be conducted. The purpose of this evaluation is to analyze the various Approaches` processes, to learn what`s working and what`s not, and to offer recommendations as to how Bonneville might improve their resources acquisition efforts. The evaluation was conducted with no preconceived biases.

  8. SSC/BCD data acquisition system proposal

    SciTech Connect

    Barsotti, E.; Bowden, M.; Swoboda, C.

    1989-04-01

    The proposed new data acquisition system architecture takes event fragments off a detector over fiber optics and to a parallel event building switch. The parallel event building switch concept, taken from the telephone communications industry, along with expected technology improvements in fiber-optic data transmission speeds over the next few years, should allow data acquisition system rates to increase dramatically and exceed those rates needed for the SSC. This report briefly describes the switch architecture and fiber optics for a SSC data acquisition system.

  9. 48 CFR 219.502 - Setting aside acquisitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Setting aside acquisitions..., DEPARTMENT OF DEFENSE SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 219.502 Setting aside acquisitions....

  10. Acquisition of Crosswell Seismic Monitoring Data

    SciTech Connect

    Daley, T.M.; Niu, F.; Silver, P.G.; Majer, E.L.

    2008-02-15

    Crosswell seismic acquisition provides an ideal geometry for monitoring travel time changes in the subsurface. Analysis of delay time in terms of a characteristic frequency allows us to estimate optimal acquisition parameters (frequency and distance). We have deployed standard data acquisition equipment for continuous monitoring of crosswell travel time in two separate field experiments, with well spacing of 3 and 30 m. The acquisition hardware used for the field experiments is described, along with environmental effects (such as temperature) that influence the measurements. Two field experiments are described that correlate changes in travel time (and therefore velocity) with changes in barometric pressure. The results from the two field sites show a pressure sensitivity for velocity of 10{sup -6}/Pa to 10{sup -8}/Pa.

  11. Generative Research on Second Language Acquisition.

    ERIC Educational Resources Information Center

    Eubank, Lynn

    1995-01-01

    Reviews recent trends in generative research on second language acquisition, focusing on the role of universal grammar, parameter resetting, and anaphoric binding. An annotated bibliography discusses five important works in the field. (61 references) (MDM)

  12. Second Language Acquisition and Language Universals Research.

    ERIC Educational Resources Information Center

    Comrie, Bernard

    1990-01-01

    Explores research and theory regarding the relation between second-language acquisition and language universals research with respect to three phenomena: extraction; structure dependence; and the distribution of overt reflexives. (Author/CB)

  13. 32 CFR 644.7 - Acquisition lines.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... their effect upon fringe tracts. It will, therefore, be necessary to establish final acquisition lines, in accordance with sound real estate practices. Accordingly, fringe tracts will not be acquired...

  14. Ethological Approach to Delayed Language Acquisition

    ERIC Educational Resources Information Center

    Mahoney, Gerald J.

    1975-01-01

    The ethological approach to language acquisition (which focuses on the nature of the child's interaction with the environment) is described, and implications for the language development of mentally retarded and autistic children are discussed. (CL)

  15. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1985-01-01

    Reports on developments in programs managed by JPL's Office of Telecommunications and Data Acquisition are presented. Emphasis is placed on activities of the Deep Space Network and its associated ground facilities.

  16. 32 CFR 644.82 - Prerequisites to acquisition.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROPERTY REAL ESTATE HANDBOOK Acquisition Acquisition by Purchase, Donation, and Transfer § 644.82..., negative declarations must be supported by written environmental assessments which generally meet the...

  17. 32 CFR 644.82 - Prerequisites to acquisition.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PROPERTY REAL ESTATE HANDBOOK Acquisition Acquisition by Purchase, Donation, and Transfer § 644.82..., negative declarations must be supported by written environmental assessments which generally meet the...

  18. 32 CFR 644.82 - Prerequisites to acquisition.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROPERTY REAL ESTATE HANDBOOK Acquisition Acquisition by Purchase, Donation, and Transfer § 644.82..., negative declarations must be supported by written environmental assessments which generally meet the...

  19. 15 CFR 921.31 - Supplemental acquisition and development awards.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Foreign Trade (Continued) NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE OCEAN... the environment. Acquisition awards for the acquisition of lands or waters, or interests therein,...

  20. 15 CFR 921.31 - Supplemental acquisition and development awards.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Foreign Trade (Continued) NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE OCEAN... the environment. Acquisition awards for the acquisition of lands or waters, or interests therein,...

  1. Updated NGNP Fuel Acquisition Strategy

    SciTech Connect

    David Petti; Tim Abram; Richard Hobbins; Jim Kendall

    2010-12-01

    A Next Generation Nuclear Plant (NGNP) fuel acquisition strategy was first established in 2007. In that report, a detailed technical assessment of potential fuel vendors for the first core of NGNP was conducted by an independent group of international experts based on input from the three major reactor vendor teams. Part of the assessment included an evaluation of the credibility of each option, along with a cost and schedule to implement each strategy compared with the schedule and throughput needs of the NGNP project. While credible options were identified based on the conditions in place at the time, many changes in the assumptions underlying the strategy and in externalities that have occurred in the interim requiring that the options be re-evaluated. This document presents an update to that strategy based on current capabilities for fuel fabrication as well as fuel performance and qualification testing worldwide. In light of the recent Pebble Bed Modular Reactor (PBMR) project closure, the Advanced Gas Reactor (AGR) fuel development and qualification program needs to support both pebble and prismatic options under the NGNP project. A number of assumptions were established that formed a context for the evaluation. Of these, the most important are: • Based on logistics associated with the on-going engineering design activities, vendor teams would start preliminary design in October 2012 and complete in May 2014. A decision on reactor type will be made following preliminary design, with the decision process assumed to be completed in January 2015. Thus, no fuel decision (pebble or prismatic) will be made in the near term. • Activities necessary for both pebble and prismatic fuel qualification will be conducted in parallel until a fuel form selection is made. As such, process development, fuel fabrication, irradiation, and testing for pebble and prismatic options should not negatively influence each other during the period prior to a decision on reactor type

  2. Target Acquisition and Positioning Study (TAPS)

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The Scientific Instruments for the Large Space Telescope (LST) require a high degree of accuracy for positioning targets within their respective entrance apertures. The acquisition, verification of position, and guidance during an experiment must be accomplished with a minimum loss of observing time for the maximum effectiveness of the total mission. This study evaluates several viable concepts and modes of operation that are applicable for a Target Acquisition and Positioning System (TAPS) that is responsive to the LST instrument requirements.

  3. Rotor fatigue monitoring data acquisition system

    NASA Technical Reports Server (NTRS)

    Smith, Scott M.

    1993-01-01

    The 40 by 80 Foot Wind Tunnel of the National Full Scale Aerodynamics Complex (NFAC) had a requirement to monitor rotor fatigue during a test. This test subjected various rotor components to stress levels higher than their structural fatigue limits. A data acquisition system was developed to monitor the cumulative fatigue damage of rotor components using National Instruments hardware and LabVIEW software. A full description of the data acquisition system including its configuration and salient features, is presented in this paper.

  4. Positron Emission Mammography with Multiple Angle Acquisition

    SciTech Connect

    Mark F. Smith; Stan Majewski; Raymond R. Raylman

    2002-11-01

    Positron emission mammography (PEM) of F-18 fluorodeoxyglucose (FDG) uptake in breast tumors with dedicated detectors typically has been accomplished with two planar detectors in a fixed position with the breast under compression. The potential use of PEM imaging at two detector positions to guide stereotactic breast biopsy has motivated us to use PEM coincidence data acquired at two or more detector positions together in a single image reconstruction. Multiple angle PEM acquisition and iterative image reconstruction were investigated using point source and compressed breast phantom acquisitions with 5, 9, 12 and 15 mm diameter spheres and a simulated tumor:background activity concentration ratio of 6:1. Image reconstruction was performed with an iterative MLEM algorithm that used coincidence events between any two detector pixels on opposed detector heads at each detector position. This present study compared two acquisition protocols: 2 angle acquisition with detector angular positions of -15 and +15 degrees and 11 angle acquisition with detector positions spaced at 3 degree increments over the range -15 to +15 degrees. Three- dimensional image resolution was assessed for the point source acquisitions, and contrast and signal-to-noise metrics were evaluated for the compressed breast phantom with different simulated tumor sizes. Radial and tangential resolutions were similar for the two protocols, while normal resolution was better for the 2 angle acquisition. Analysis is complicated by the asymmetric point spread functions. Signal- to-noise vs. contrast tradeoffs were better for 11 angle acquisition for the smallest visible 9 mm sphere, while tradeoff results were mixed for the larger and more easily visible 12 mm and 15 mm diameter spheres. Additional study is needed to better understand the performance of limited angle tomography for PEM. PEM tomography experiments with complete angular sampling are planned.

  5. Positron Emission Mammography with Multiple Angle Acquisition

    SciTech Connect

    Mark F. Smith; Stan Majewski; Raymond R. Raylman

    2002-11-01

    Positron emission mammography (PEM) of F-18 fluorodeoxyglucose (FbG) uptake in breast tumors with dedicated detectors typically has been accomplished with two planar detectors in a fixed position with the breast under compression. The potential use of PEM imaging at two detector positions to guide stereotactic breast biopsy has motivated us to use PEM coincidence data acquired at two or more detector positions together in a single image reconstruction. Multiple angle PEM acquisition and iterative image reconstruction were investigated using point source and compressed breast phantom acquisitions with 5, 9, 12 and 15 mm diameter spheres and a simulated tumor:background activity concentration ratio of 6:1. Image reconstruction was performed with an iterative MLEM algorithm that used coincidence events between any two detector pixels on opposed detector heads at each detector position. This present study compared two acquisition protocols: 2 angle acquisition with detector angular positions of -15 and +15 degrees and 11 angle acquisition with detector positions spaced at 3 degree increments over the range -15 to +15 degrees. Three-dimensional image resolution was assessed for the point source acquisitions, and contrast and signal-to-noise metrics were evaluated for the compressed breast phantom with different simulated tumor sizes. Radial and tangential resolutions were similar for the two protocols, while normal resolution was better for the 2 angle acquisition. Analysis is complicated by the asymmetric point spread functions. Signal- to-noise vs. contrast tradeoffs were better for 11 angle acquisition for the smallest visible 9 mm sphere, while tradeoff results were mixed for the larger and more easily visible 12 mm and 15 mm diameter spheres. Additional study is needed to better understand the performance of limited angle tomography for PEM. PEM tomography experiments with complete angular sampling are planned.

  6. Thermal (Silicon Diode) Data Acquisition Systems

    NASA Technical Reports Server (NTRS)

    Wright, Ernest; Kegley, Jeff

    2008-01-01

    Marshall Space Flight Center s X-ray Cryogenic Facility (XRCF) has been performing cryogenic testing to 20 Kelvin since 1999. Two configurations for acquiring data from silicon diode temperature sensors have been implemented at the facility. The facility's environment is recorded via a data acquisition system capable of reading up to 60 silicon diodes. Test article temperature is recorded by a second data acquisition system capable of reading 150+ silicon diodes. The specifications and architecture of both systems will be presented.

  7. Thermal (Silicon Diode) Data Acquisition System

    NASA Technical Reports Server (NTRS)

    Kegley, Jeffrey

    2008-01-01

    Marshall Space Flight Center's X-ray Calibration Facility (XRCF) has been performing cryogenic testing to 20 Kelvin since 1999. Two configurations for acquiring data from silicon diode temperature sensors have been implemented at the facility. The facility's environment is recorded via a data acquisition system capable of reading up to 60 silicon diodes. Test article temperature is recorded by a second data acquisition system capable of reading 150+ silicon diodes. The specifications and architecture of both systems will be presented.

  8. Measurement and Controls Data Acquisition System

    NASA Technical Reports Server (NTRS)

    Hall, Rick; Daniel, Alice; Batts, Frank E., Sr.

    2006-01-01

    Measurement and Controls Data Acquisition System (MCDAS) is an application program that integrates the functions of two stand-alone programs: one for acquisition of data, the other for controls. MCDAS facilitates and improves testing of complex engineering systems by helping to perform calibration and setup of test systems and acquisition, dissemination, and processing of data. Features of MCDAS include an intuitive, user-friendly graphical user interface, a capability for acquiring data at rates greater than previously possible, cooperation between the data-acquisition software subsystem and alarm-checking and analytical components of the control software subsystem, and a capability for dissemination of data through fiber optics and virtual and wide-area networks, including networks that contain hand-held display units. The integration of the data acquisition and control software offers a safety advantage by making alarm information available to the control software in a more timely manner. By enabling the use of hand-held devices, MCDAS reduces the time spent by technicians asking for screen updates to determine effects of setup actions. Previously recorded data can be processed without interruption to current acquisition of data. Analysts can continue to view test parameters while test-data files are being generated.

  9. Foreign DNA acquisition by the I-F CRISPR–Cas system requires all components of the interference machinery

    PubMed Central

    Vorontsova, Daria; Datsenko, Kirill A.; Medvedeva, Sofia; Bondy-Denomy, Joseph; Savitskaya, Ekaterina E.; Pougach, Ksenia; Logacheva, Maria; Wiedenheft, Blake; Davidson, Alan R.; Severinov, Konstantin; Semenova, Ekaterina

    2015-01-01

    CRISPR immunity depends on acquisition of fragments of foreign DNA into CRISPR arrays. For type I-E CRISPR–Cas systems two modes of spacer acquisition, naïve and primed adaptation, were described. Naïve adaptation requires just two most conserved Cas1 and Cas2 proteins; it leads to spacer acquisition from both foreign and bacterial DNA and results in multiple spacers incapable of immune response. Primed adaptation requires all Cas proteins and a CRISPR RNA recognizing a partially matching target. It leads to selective acquisition of spacers from DNA molecules recognized by priming CRISPR RNA, with most spacers capable of protecting the host. Here, we studied spacer acquisition by a type I-F CRISPR–Cas system. We observe both naïve and primed adaptation. Both processes require not just Cas1 and Cas2, but also intact Csy complex and CRISPR RNA. Primed adaptation shows a gradient of acquisition efficiency as a function of distance from the priming site and a strand bias that is consistent with existence of single-stranded adaption intermediates. The results provide new insights into the mechanism of spacer acquisition and illustrate surprising mechanistic diversity of related CRISPR–Cas systems. PMID:26586803

  10. Structural basis of haem-iron acquisition by fungal pathogens.

    PubMed

    Nasser, Lena; Weissman, Ziva; Pinsky, Mariel; Amartely, Hadar; Dvir, Hay; Kornitzer, Daniel

    2016-01-01

    Pathogenic microorganisms must cope with extremely low free-iron concentrations in the host's tissues. Some fungal pathogens rely on secreted haemophores that belong to the Common in Fungal Extracellular Membrane (CFEM) protein family, to extract haem from haemoglobin and to transfer it to the cell's interior, where it can serve as a source of iron. Here we report the first three-dimensional structure of a CFEM protein, the haemophore Csa2 secreted by Candida albicans. The CFEM domain adopts a novel helical-basket fold that consists of six α-helices, and is uniquely stabilized by four disulfide bonds formed by its eight signature cysteines. The planar haem molecule is bound between a flat hydrophobic platform located on top of the helical basket and a peripheral N-terminal 'handle' extension. Exceptionally, an aspartic residue serves as the CFEM axial ligand, and so confers coordination of Fe(3+) haem, but not of Fe(2+) haem. Histidine substitution mutants of this conserved Asp acquired Fe(2+) haem binding and retained the capacity to extract haem from haemoglobin. However, His-substituted CFEM proteins were not functional in vivo and showed disturbed haem exchange in vitro, which suggests a role for the oxidation-state-specific Asp coordination in haem acquisition by CFEM proteins. PMID:27617569

  11. Targeted gene transfer into ependymal cells through intraventricular injection of AAV1 vector and long-term enzyme replacement via the CSF.

    PubMed

    Yamazaki, Yoshiyuki; Hirai, Yukihiko; Miyake, Koichi; Shimada, Takashi

    2014-01-01

    Enzyme replacement via the cerebrospinal fluid (CSF) has been shown to ameliorate neurological symptoms in model animals with neuropathic metabolic disorders. Gene therapy via the CSF offers a means to achieve a long-term sustainable supply of therapeutic proteins within the central nervous system (CNS) by setting up a continuous source of transgenic products. In the present study, a serotype 1 adeno-associated virus (AAV1) vector was injected into a lateral cerebral ventricle in adult mice to transduce the gene encoding human lysosomal enzyme arylsulfatase A (hASA) into the cells of the CNS. Widespread transduction and stable expression of hASA in the choroid plexus and ependymal cells was observed throughout the ventricles for more than 1 year after vector injection. Although humoral immunity to hASA developed after 6 weeks, which diminished the hASA levels detected in CSF from AAV1-injected mice, hASA levels in CSF were maintained for at least 12 weeks when the mice were tolerized to hASA prior of vector injection. Our results suggest that the cells lining the ventricles could potentially serve as a biological reservoir for long-term continuous secretion of lysosomal enzymes into the CSF following intracerebroventricular injection of an AAV1 vector. PMID:24981028

  12. NIR daylight acquisition sensor improves mission capabilities

    NASA Astrophysics Data System (ADS)

    Chesser, Douglas E.; Vunck, Darius; Born, Terry; Axelson, Wayne; Rehder, Karl; Medrano, Robert S.

    2003-08-01

    The US Air Force Maui Space Surveillance System includes a 1.6 meter telescope located at the summit of Haleakala. This telescope has long played a key role in Space Object Identification (SOI) and other scientific research projects. The unique configuration of the 1.6m telescope and its suite of instruments make it ideally suited for high speed, extra-atmospheric Satellite and Missile tracking. However, because of the uniquely designed narrow field of the 1.6m telescope, acquisition of daytime objects presents a challenge. In the past, the 1.6 meter system relied primarily on offsite radar handoffs to provide FOV object placement. This reliance on radar based handoffs increased system operational complexity and decreased system reliability. Recognizing the value of improving mission operational availability and success the US Air Force Research Laboratory and contractor Boeing worked together to design a low cost system to improve the wide field acquisition of daylight objects. This instrument, known as the Daylight Acquisition Sensor (DAS), was developed using a COTS NIR Camera with custom NIR optics assemblies controlled with an integrated COTS embedded computer interface. The design that was implemented is a modification to the existing 0.56 meter nighttime only acquisition telescope, which now, because of the new NIR imaging sensor is capable of both daytime and nighttime acquisition. The system has been in operation for over 1 year and has significantly improved the acquisition capabilities of the 1.6m telescope while at the same time greatly reducing dependency on radar handoff. This paper discusses the design of the NIR Daylight Acquisition Sensor and some of the results from missions it has supported.

  13. 48 CFR 1319.502 - Setting aside acquisitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Setting aside acquisitions. 1319.502 Section 1319.502 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 1319.502 Setting aside acquisitions....

  14. 48 CFR 819.502 - Setting aside acquisitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Setting aside acquisitions. 819.502 Section 819.502 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 819.502 Setting aside acquisitions....

  15. 48 CFR 907.105 - Contents of written acquisition plans.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contents of written acquisition plans. 907.105 Section 907.105 Federal Acquisition Regulations System DEPARTMENT OF ENERGY... acquisition plans. (b)(16) Environmental and energy conservation objectives. Incorporate sustainable...

  16. 48 CFR 939.7002 - Contractor acquisition of information technology.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Contractor acquisition of information technology. 939.7002 Section 939.7002 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SPECIAL CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY Implementing DOE Policies and Procedures 939.7002...

  17. 10 CFR 626.4 - General acquisition strategy.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... DEPARTMENT OF ENERGY (CONTINUED) SALES REGULATION PROCEDURES FOR ACQUISITION OF PETROLEUM FOR THE STRATEGIC PETROLEUM RESERVE § 626.4 General acquisition strategy. (a) Criteria for commencing acquisition. To reduce... prior to commencing acquisition of petroleum for the SPR: (1) The current inventory of the SPR; (2)...

  18. 10 CFR 626.4 - General acquisition strategy.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... DEPARTMENT OF ENERGY (CONTINUED) SALES REGULATION PROCEDURES FOR ACQUISITION OF PETROLEUM FOR THE STRATEGIC PETROLEUM RESERVE § 626.4 General acquisition strategy. (a) Criteria for commencing acquisition. To reduce... prior to commencing acquisition of petroleum for the SPR: (1) The current inventory of the SPR; (2)...

  19. 10 CFR 626.4 - General acquisition strategy.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... DEPARTMENT OF ENERGY (CONTINUED) SALES REGULATION PROCEDURES FOR ACQUISITION OF PETROLEUM FOR THE STRATEGIC PETROLEUM RESERVE § 626.4 General acquisition strategy. (a) Criteria for commencing acquisition. To reduce... prior to commencing acquisition of petroleum for the SPR: (1) The current inventory of the SPR; (2)...

  20. 48 CFR 208.7003-2 - Assignments under coordinated acquisition.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ordnance regardless of agency control, or to any item which requires test or inspection conducted or... coordinated acquisition. 208.7003-2 Section 208.7003-2 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES...

  1. 48 CFR 208.7003-2 - Assignments under coordinated acquisition.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ordnance regardless of agency control, or to any item which requires test or inspection conducted or... coordinated acquisition. 208.7003-2 Section 208.7003-2 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES...

  2. 48 CFR 208.7003-2 - Assignments under coordinated acquisition.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ordnance regardless of agency control, or to any item which requires test or inspection conducted or... coordinated acquisition. 208.7003-2 Section 208.7003-2 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES...

  3. 48 CFR 208.7003-2 - Assignments under coordinated acquisition.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ordnance regardless of agency control, or to any item which requires test or inspection conducted or... coordinated acquisition. 208.7003-2 Section 208.7003-2 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES...

  4. A Research on Second Language Acquisition and College English Teaching

    ERIC Educational Resources Information Center

    Li, Changyu

    2009-01-01

    It was in the 1970s that American linguist S.D. Krashen created the theory of "language acquisition". The theories on second language acquisition were proposed based on the study on the second language acquisition process and its rules. Here, the second language acquisition process refers to the process in which a learner with the…

  5. 26 CFR 1.279-3 - Corporate acquisition indebtedness.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TAX (CONTINUED) INCOME TAXES Items Not Deductible § 1.279-3 Corporate acquisition indebtedness. (a) Corporate acquisition indebtedness. For purposes of section 279, the term corporate acquisition indebtedness... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Corporate acquisition indebtedness....

  6. 48 CFR 37.114 - Special acquisition requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... those that involve support of government policy or decision making. During performance of service... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Special acquisition requirements. 37.114 Section 37.114 Federal Acquisition Regulations System FEDERAL ACQUISITION...

  7. 48 CFR 37.114 - Special acquisition requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... those that involve support of government policy or decision making. During performance of service... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Special acquisition requirements. 37.114 Section 37.114 Federal Acquisition Regulations System FEDERAL ACQUISITION...

  8. 48 CFR 37.114 - Special acquisition requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... those that involve support of government policy or decision making. During performance of service... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Special acquisition requirements. 37.114 Section 37.114 Federal Acquisition Regulations System FEDERAL ACQUISITION...

  9. 48 CFR 37.114 - Special acquisition requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... those that involve support of government policy or decision making. During performance of service... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Special acquisition requirements. 37.114 Section 37.114 Federal Acquisition Regulations System FEDERAL ACQUISITION...

  10. Second Language Acquisition by Adult Immigrants: Exemplified Methodology.

    ERIC Educational Resources Information Center

    Trevise, Anne; Porquier, Remy

    1986-01-01

    Illustrates some of the problems of second language (French) acquisition by adult Spanish-speaking immigrants in a natural setting through analysis of the European Project data (Perdue, 1982) in three areas: acquisition and use of the over-generalized marker "c'est"; acquisition of reference to time; and acquisition of reference to space.…

  11. 49 CFR 24.102 - Basic acquisition policies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Basic acquisition policies. 24.102 Section 24.102... ACQUISITION FOR FEDERAL AND FEDERALLY-ASSISTED PROGRAMS Real Property Acquisition § 24.102 Basic acquisition... the owner in writing of the Agency's interest in acquiring the real property and the basic...

  12. 18 CFR 157.16 - Exhibits relating to acquisitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Sales, Service, Construction, Extension, Acquisition or Abandonment § 157.16 Exhibits relating to acquisitions. In addition to the exhibits required by § 157.14, every application involving acquisition of... follow the procedures set out in § 157.10(d). (a) Exhibit Q—Effect of acquisition on existing...

  13. Women's beliefs concerning condom acquisition and use.

    PubMed

    Libbus, K

    1995-10-01

    Condoms are a time-honored and reliable method of protection against pregnancy and sexually transmitted infections. However, their use, and thus their effectiveness, is determined by individual behavior. The purpose of this paper is to report attitudes and salient beliefs related to condom use in a sample of adult women. The study used Ajzen and Fishbein's Theory of Planned Behavior to identify modal, salient beliefs regarding condom acquisition and use as intentional behaviors. The study sample consisted of 58 community women who reported using condoms for contraceptive purposes within the last five years. In face-to-face, audiotaped interviews, open-ended questions were used to solicit beliefs regarding condom acquisition and use. All subject narratives were content-analyzed for recurrent themes. Women cited accessibility and effectiveness in preventing pregnancy and sexually transmitted diseases as both advantages and as factors contributing to the ease of acquisition and use. Disadvantages and factors that might deter condom acquisition and use included embarrassment, objections by male partner, and effect on spontaneity. Overall, subjects exhibited accurate knowledge regarding the benefits of condom acquisition and use. However, it is possible that expressed negative beliefs could take precedence in decision-making and reduce the probability of consistent condom use. PMID:7479543

  14. 75 FR 13413 - Federal Acquisition Regulation; FAR Case 2009-035, Extend Use of Simplified Acquisition...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... Federal Acquisition Regulation; FAR Case 2009-035, Extend Use of Simplified Acquisition Procedures for... 137; and 42 U.S.C. 2473(c). 13.500 0 2. Amend section 13.500 by removing from paragraph (d) ``January... cite FAC 2005-39, FAR case 2009-035. SUPPLEMENTARY INFORMATION: A. Background This final rule...

  15. 75 FR 45072 - Defense Federal Acquisition Regulation Supplement; Inflation Adjustment of Acquisition-Related...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-02

    ... (71 FR 75891). This is the second review of DFARS acquisition-related thresholds. DoD published a proposed rule on January 20, 2010 (75 FR 3187). The preamble to the proposed rule contained a detailed...; Inflation Adjustment of Acquisition-Related Thresholds (DFARS Case 2009-D003) AGENCY: Defense...

  16. 75 FR 6668 - Federal Acquisition Regulation; Submission for OMB Review; Commercial Item Acquisitions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-10

    ... in commercial item acquisitions. A request for public comments was published in the Federal Register at 74 FR 58628, on November 13, 2009. No comments were received. Public comments are particularly... Regulation; Submission for OMB Review; Commercial Item Acquisitions AGENCIES: Department of Defense...

  17. 77 FR 56744 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-61; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ...This document is issued under the joint authority of DOD, GSA, and NASA. This Small Entity Compliance Guide has been prepared in accordance with section 212 of the Small Business Regulatory Enforcement Fairness Act of 1996. It consists of a summary of the rule appearing in Federal Acquisition Circular (FAC) 2005-61, which amends the Federal Acquisition Regulation (FAR). An asterisk (*) next to......

  18. 76 FR 4191 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-49; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-24

    ... rules appearing in Federal Acquisition Circular (FAC) 2005-49, which amend the Federal Acquisition Regulation (FAR). Interested parties may obtain further information regarding these rules by referring to FAC... CONTACT: The analyst whose name appears in the table below. Please cite FAC 2005-49 and the specific...

  19. 77 FR 30365 - Defense Federal Acquisition Regulation Supplement: Report on Waiver of Prohibition on Acquisition...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-22

    ... Regulation Supplement: Report on Waiver of Prohibition on Acquisition From Communist Chinese Military... prohibition on acquisition of United States Munitions List items from Communist Chinese military companies... Chinese military companies. The prior requirement was for a report within 30 days after the date of...

  20. 76 FR 18303 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-51; Introduction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ...-51; Introduction; Women-Owned Small Business (WOSB) Program; Clarification of Standard Form 26--Award...; Federal Acquisition Circular 2005-51; Introduction AGENCIES: Department of Defense (DoD), General Services...) rules agreed to by DoD, GSA, and NASA in this Federal Acquisition Circular (FAC) 2005-51. A...

  1. 77 FR 69713 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-62; Introduction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-20

    ..., and NASA published a proposed rule in the Federal Register at 76 FR 73564 on November 29, 2011 to... Acquisition Regulation; Federal Acquisition Circular 2005-62; Introduction AGENCIES: Department of Defense (Do... addition to complying with FAR subpart 17.5. To provide clarification for small business and...

  2. 77 FR 23363 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-58; Introduction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ... adopts as final, without change, an interim rule published in the Federal Register at 76 FR 14559 on... Federal Acquisition Regulation; Federal Acquisition Circular 2005-58; Introduction AGENCY: Department of... biobased products purchased for each contract. However, this rule may enhance small business...

  3. 78 FR 6183 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-65; Introduction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ... Register at 77 FR 27548 on May 10, 2012, to implement the United States-Colombia Trade Promotion Agreement... Federal Acquisition Regulation; Federal Acquisition Circular 2005-65; Introduction AGENCY: Department of... and wants to do business with the Government. Small business concerns are unlikely to have...

  4. 77 FR 27545 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-59; Introduction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... Acquisition Regulation; Federal Acquisition Circular 2005-59; Introduction AGENCY: Department of Defense (DoD... at FAR 9.108-2. This rule is not expected to have an effect on small business because this rule will only impact an offeror that is an inverted domestic corporation and wants to do business with...

  5. Managing Uncertainty during a Corporate Acquisition: A Longitudinal Study of Communication During an Airline Acquisition

    ERIC Educational Resources Information Center

    Kramer, Michael W.; Dougherty, Debbie S.; Pierce, Tamyra A.

    2004-01-01

    This study examined pilots' (N at T1 = 140; N at T2 = 126; N at T3 = 104) reactions to communication and uncertainty during the acquisition of their airline by another airline. Quantitative results indicate that communication helped to reduce uncertainty and was predictive of affective responses to the acquisition. However, contrary to…

  6. 76 FR 21810 - Defense Federal Acquisition Regulation Supplement; Acquisition of Commercial Items (DFARS Case...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-19

    ... a FAR part 15-type solicitation for commercial items and require the offeror to request an exception... Acquisition Regulation Supplement; Acquisition of Commercial Items (DFARS Case 2008-D011) AGENCY: Department.... SUPPLEMENTARY INFORMATION: I. Background DoD published an interim rule at 74 FR 34263 on July 15, 2009...

  7. 78 FR 13769 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-66; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... Acquisition Procedures for Certain Commercial Items. IV Technical Amendments SUPPLEMENTARY INFORMATION...--Extension of Authority for Use of Simplified Acquisition Procedures for Certain Commercial Items (FAR Case... Act (NDAA) for Fiscal Year (FY) 2013. Section 822 extends the authority of the Commercial Item...

  8. 78 FR 13763 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-66; Introduction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... Acquisition Procedures for Certain Commercial Items. IV Technical Amendments. SUPPLEMENTARY INFORMATION...--Extension of Authority for Use of Simplified Acquisition Procedures for Certain Commercial Items (FAR Case... Act (NDAA) for Fiscal Year (FY) 2013. Section 822 extends the authority of the Commercial Item...

  9. 76 FR 44280 - Defense Federal Acquisition Regulation Supplement; Simplified Acquisition Threshold for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ... Regulation Supplement; Simplified Acquisition Threshold for Humanitarian or Peacekeeping Operations (DFARS... humanitarian or peacekeeping operation. The current simplified acquisition threshold is $150,000 as specified... against the United States; and (c) In support of a humanitarian or peacekeeping operation. The first...

  10. 76 FR 71833 - Defense Federal Acquisition Regulation Supplement: Simplified Acquisition Threshold for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ... in the Federal Register at 76 FR 44280 on July 25, 2011, to amend the Defense Federal Acquisition... amending 48 CFR parts 202 and 218, which was published at 76 FR 44280 on July 25, 2011, is adopted as a... Regulation Supplement: Simplified Acquisition Threshold for Humanitarian or Peacekeeping Operations...

  11. 78 FR 13544 - Defense Federal Acquisition Regulation Supplement: Acquisition of Tents and Other Temporary...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... in the Federal Register (77 FR 38734) on June ] 29, 2012, to implement sections 368 and 821 of the... amending 48 CFR parts 215, 225, and 252 which was published at 77 FR 38734 on June 29, 2012, is adopted as... Acquisition Regulation Supplement: Acquisition of Tents and Other Temporary Structures (DFARS Case...

  12. 77 FR 38734 - Defense Federal Acquisition Regulation Supplement; Acquisition of Tents and Other Temporary...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-29

    ... Regulation Supplement; Acquisition of Tents and Other Temporary Structures (DFARS Case 2012-D015) AGENCY... 2012 that address the acquisition of tents and other temporary structures. DATES: Effective date: June... the best value, when acquiring tents and other temporary structures, regardless of whether...

  13. 76 FR 68044 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-54; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-02

    ... 75 FR 77723 on December 13, 2010, implementing Executive Order (E.O.) 13496, Notification of Employee...) regulations. The FAR interim rule was published in the Federal Register at 75 FR 77737 on December 13, 2010... Acquisition Regulation; Federal Acquisition Circular 2005-54; Small Entity Compliance Guide AGENCY:...

  14. Societal Responses to Adult Difficulties in L2 Acquisition: Toward an Evolutionary Perspective on Language Acquisition

    ERIC Educational Resources Information Center

    Schumann, John H.

    2013-01-01

    It is generally accepted that second language (L2) acquisition becomes more difficult as one grows older and that success in adult L2 acquisition is highly variable. Nevertheless, humans in language contact situations have to cope with intergroup communication. This article examines the ways society has responded to this challenge. It describes…

  15. Grammatical Acquisition: Inductive Bias and Coevolution of Language and the Language Acquisition Device.

    ERIC Educational Resources Information Center

    Briscoe, Ted

    2000-01-01

    An account of grammatical acquisition is developed within the parameter setting framework applied to a generalized categorical grammar (GCG). Computational simulation shows that several resulting acquisition procedures are effective on a parameter set expressing major typological distinctions based on constituent order, and defining 70 distinct…

  16. Sequential acquisition of multi-dimensional heteronuclear chemical shift correlation spectra with 1H detection

    PubMed Central

    Bellstedt, Peter; Ihle, Yvonne; Wiedemann, Christoph; Kirschstein, Anika; Herbst, Christian; Görlach, Matthias; Ramachandran, Ramadurai

    2014-01-01

    RF pulse schemes for the simultaneous acquisition of heteronuclear multi-dimensional chemical shift correlation spectra, such as {HA(CA)NH & HA(CACO)NH}, {HA(CA)NH & H(N)CAHA} and {H(N)CAHA & H(CC)NH}, that are commonly employed in the study of moderately-sized protein molecules, have been implemented using dual sequential 1H acquisitions in the direct dimension. Such an approach is not only beneficial in terms of the reduction of experimental time as compared to data collection via two separate experiments but also facilitates the unambiguous sequential linking of the backbone amino acid residues. The potential of sequential 1H data acquisition procedure in the study of RNA is also demonstrated here. PMID:24671105

  17. 48 CFR 46.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Government contract... Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY... acquisition threshold at destination and only for type and kind; quantity; damage; operability (if...

  18. 48 CFR 46.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Government contract... Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY... acquisition threshold at destination and only for type and kind; quantity; damage; operability (if...

  19. 75 FR 5716 - Federal Acquisition Regulation; FAR Case 2008-024, Inflation Adjustment of Acquisition-Related...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-04

    ... the Federal Register on September 28, 2006 (71 FR 57363). B. Analysis 1. What is an acquisition... Acquisition Regulation; FAR Case 2008-024, Inflation Adjustment of Acquisition-Related Thresholds AGENCIES... Act requires an adjustment every 5 years of acquisition- related thresholds for inflation using...

  20. 48 CFR 246.404 - Government contract quality assurance for acquisitions at or below the simplified acquisition...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Government Contract Quality Assurance 246.404 Government contract quality assurance for acquisitions at or below the simplified acquisition threshold. Do not... quality assurance for acquisitions at or below the simplified acquisition threshold. 246.404 Section...

  1. Sample Acquisition Techniques for Exobiology Flight Experiments

    NASA Technical Reports Server (NTRS)

    Kojiro, Daniel R.; Carle, Glenn C.; Stratton, David M.; Valentin, Jose R.; DeVincenzi, Donald (Technical Monitor)

    1999-01-01

    Exobiology Flight Experiments involve complex analyses conducted in environments far different than those encountered in terrestrial applications. A major part of the analytical challenge is often the selection, acquisition, delivery and, in some cases, processing of a sample suitable for the analytical requirements of the mission. The added complications of severely limited resources and sometimes rigid time constraints combine to make sample acquisition potentially a major obstacle for successful analyses. Potential samples come in a wide range including planetary atmospheric gas and aerosols (from a wide variety of pressures), planetary soil or rocks, dust and ice particles streaming off of a comet, and cemetery surface ice and rocks. Methods to collect and process sample are often mission specific, requiring continual development of innovative concepts and mechanisms. These methods must also maintain the integrity of the sample for the experimental results to be meaningful. We present here sample acquisition systems employed from past missions and proposed for future missions.

  2. Spectrum acquisition of detonation based on CMOS

    NASA Astrophysics Data System (ADS)

    Li, Yan; Bai, Yonglin; Wang, Bo; Liu, Baiyu; Xue, Yingdong; Zhang, Wei; Gou, Yongsheng; Bai, Xiaohong; Qin, Junjun; Xian, Ouyang

    2010-10-01

    The detection of high-speed dynamic spectrum is the main method to acquire transient information. In order to obtain the large amount spectral data in real-time during the process of detonation, a CMOS-based system with high-speed spectrum data acquisition is designed. The hardware platform of the system is based on FPGA, and the unique characteristic of CMOS image sensors in the rolling shutter model is used simultaneously. Using FPGA as the master control chip of the system, not only provides the time sequence for CIS, but also controls the storage and transmission of the spectral data. In the experiment of spectral data acquisition, the acquired information is transmitted to the host computer through the CameraLink bus. The dynamic spectral curve is obtained after the subsequent processing. The experimental results demonstrate that this system is feasible in the acquisition and storage of high-speed dynamic spectrum information during the process of detonation.

  3. DAQMAN - A flexible configurable data acquisition system

    SciTech Connect

    Sivertz, Michael; Larry Hoff, Seth Nemesure

    2012-08-01

    DAQMAN is a flexible configurable interface that allows the user to build and operate a VME-based data acquisition system on a Linux workstation. It consists of two parts: a Java-based Graphical User Interface to configure the system, and a C-based utility that reads out the data and creates the output ASCII data file, with two levels of diagnostic tools. The data acquisition system requires a CAEN CONET-VME Bridge to communicate between the hardware in the VME crate and the Linux workstation. Data acquisition modules, such as ADCs, TDC, Scalers, can be loaded into the system, or removed easily. The GUI allows users to activate modules, and channels within modules by clicking on icons. Running configurations are stored; data are collected and can be viewed either as raw numbers, or by charts and histograms that update as the data are accumulated. Data files are written to disk in ASCII format, with a date and time stamp.

  4. Hunger promotes acquisition of nonfood objects

    PubMed Central

    Xu, Alison Jing; Schwarz, Norbert; Wyer, Robert S.

    2015-01-01

    Hunger motivates people to consume food, for which finding and acquiring food is a prerequisite. We test whether the acquisition component spills over to nonfood objects: Are hungry people more likely to acquire objects that cannot satisfy their hunger? Five laboratory and field studies show that hunger increases the accessibility of acquisition-related concepts and the intention to acquire not only food but also nonfood objects. Moreover, people act on this intention and acquire more nonfood objects (e.g., binder clips) when they are hungry, both when these items are freely available and when they must be paid for. However, hunger does not influence how much they like nonfood objects. We conclude that a basic biologically based motivation can affect substantively unrelated behaviors that cannot satisfy the motivation. This presumably occurs because hunger renders acquisition-related concepts and behaviors more accessible, which influences decisions in situations to which they can be applied. PMID:25730858

  5. Optimisation of acquisition time in bioluminescence imaging

    NASA Astrophysics Data System (ADS)

    Taylor, Shelley L.; Mason, Suzannah K. G.; Glinton, Sophie; Cobbold, Mark; Styles, Iain B.; Dehghani, Hamid

    2015-03-01

    Decreasing the acquisition time in bioluminescence imaging (BLI) and bioluminescence tomography (BLT) will enable animals to be imaged within the window of stable emission of the bioluminescent source, a higher imaging throughput and minimisation of the time which an animal is anaesthetised. This work investigates, through simulation using a heterogeneous mouse model, two methods of decreasing acquisition time: 1. Imaging at fewer wavelengths (a reduction from five to three); and 2. Increasing the bandwidth of filters used for imaging. The results indicate that both methods are viable ways of decreasing the acquisition time without a loss in quantitative accuracy. Importantly, when choosing imaging wavelengths, the spectral attenuation of tissue and emission spectrum of the source must be considered, in order to choose wavelengths at which a high signal can be achieved. Additionally, when increasing the bandwidth of the filters used for imaging, the bandwidth must be accounted for in the reconstruction algorithm.

  6. Implicit learning and acquisition of music.

    PubMed

    Rohrmeier, Martin; Rebuschat, Patrick

    2012-10-01

    Implicit learning is a core process for the acquisition of a complex, rule-based environment from mere interaction, such as motor action, skill acquisition, or language. A body of evidence suggests that implicit knowledge governs music acquisition and perception in nonmusicians and musicians, and that both expert and nonexpert participants acquire complex melodic, harmonic, and other features from mere exposure. While current findings and computational modeling largely support the learning of chunks, some results indicate learning of more complex structures. Despite the body of evidence, more research is required to support the cross-cultural validity of implicit learning and to show that core and more complex music theoretical features are acquired implicitly. PMID:23060126

  7. Magnetic Field Response Measurement Acquisition System

    NASA Technical Reports Server (NTRS)

    Woodard, Stanley E.; Taylor, Bryant D.; Shams, Qamar A.; Fox, Robert L.

    2005-01-01

    A measurement acquisition method that alleviates many shortcomings of traditional measurement systems is presented in this paper. The shortcomings are a finite number of measurement channels, weight penalty associated with measurements, electrical arcing, wire degradations due to wear or chemical decay and the logistics needed to add new sensors. The key to this method is the use of sensors designed as passive inductor-capacitor circuits that produce magnetic field responses. The response attributes correspond to states of physical properties for which the sensors measure. A radio frequency antenna produces a time-varying magnetic field used to power the sensor and receive the magnetic field response of the sensor. An interrogation system for discerning changes in the sensor response is presented herein. Multiple sensors can be interrogated using this method. The method eliminates the need for a data acquisition channel dedicated to each sensor. Methods of developing magnetic field response sensors and the influence of key parameters on measurement acquisition are discussed.

  8. Evolutionary analysis of iron (Fe) acquisition system in Marchantia polymorpha.

    PubMed

    Lo, Jing-Chi; Tsednee, Munkhtsetseg; Lo, Ying-Chu; Yang, Shun-Chung; Hu, Jer-Ming; Ishizaki, Kimitsune; Kohchi, Takayuki; Lee, Der-Chuen; Yeh, Kuo-Chen

    2016-07-01

    To acquire appropriate iron (Fe), vascular plants have developed two unique strategies, the reduction-based strategy I of nongraminaceous plants for Fe(2+) and the chelation-based strategy II of graminaceous plants for Fe(3+) . However, the mechanism of Fe uptake in bryophytes, the earliest diverging branch of land plants and dominant in gametophyte generation is less clear. Fe isotope fractionation analysis demonstrated that the liverwort Marchantia polymorpha uses reduction-based Fe acquisition. Enhanced activities of ferric chelate reductase and proton ATPase were detected under Fe-deficient conditions. However, M. polymorpha did not show mugineic acid family phytosiderophores, the key components of strategy II, or the precursor nicotianamine. Five ZIP (ZRT/IRT-like protein) homologs were identified and speculated to be involved in Fe uptake in M. polymorpha. MpZIP3 knockdown conferred reduced growth under Fe-deficient conditions, and MpZIP3 overexpression increased Fe content under excess Fe. Thus, a nonvascular liverwort, M. polymorpha, uses strategy I for Fe acquisition. This system may have been acquired in the common ancestor of land plants and coopted from the gametophyte to sporophyte generation in the evolution of land plants. PMID:26948158

  9. The data acquisition system for SLD

    SciTech Connect

    Sherden, D.J.

    1986-10-01

    This paper describes the data acquisition system planned for the SLD detector, which is being constructed for use with the SLAC Linear Collider (SLC). Analog electronics, heavily incorporating hybrid and custom VLSI circuitry, is mounted on the detector itself. Extensive use is made of multiplexing through optical fibers to a FASTBUS readout system. The low repetition rate of the SLC allows a relatively simple software-based trigger. Hardware and software processors within the acquisition modules are used to reduce the large volume of data per event and to calibrate the electronics. A farm of microprocessors is used for full reconstruction of a sample of events prior to transmission to the host.

  10. Modulated acquisition of spatial distortion maps.

    PubMed

    Volkov, Alexey; Gros, Jerneja Zganec; Zganec, Mario; Javornik, Tomaž; Svigelj, Aleš

    2013-01-01

    This work discusses a novel approach to image acquisition which improves the robustness of captured data required for 3D range measurements. By applying a pseudo-random code modulation to sequential acquisition of projected patterns the impact of environmental factors such as ambient light and mutual interference is significantly reduced. The proposed concept has been proven with an experimental range sensor based on the laser triangulation principle. The proposed design can potentially enhance the use of this principle to a variety of outdoor applications, such as autonomous vehicles, pedestrians' safety, collision avoidance, and many other tasks, where robust real-time distance detection in real world environment is crucial. PMID:23966196

  11. Let`s talk: Acquisitions and communications

    SciTech Connect

    1998-10-01

    This pamphlet describes the Department of Energy`s policies and practices to strengthen effective communications within the Department and continue the Department`s outreach efforts to its current and prospective contractors. Also, the pamphlet summarizes the Department`s approaches to providing general information, prospective acquisition information, communications during the acquisition process, and communications strategies. In creating this pamphlet, not only have the Department`s policies been delineated, but, good business practices have been liberally borrowed from other sources outside the Department. A bibliography is included at the end of this pamphlet which identifies all sources.

  12. Preschool foundations of early reading acquisition

    PubMed Central

    Rvachew, Susan; Savage, Robert

    2006-01-01

    The present paper describes research on the skills and processes associated with word and text reading acquisition in preschool children and during the first years of school. The aim is to provide an overview that gives a sense of the important milestones in language and literacy acquisition. A comparison of children’s performances against these milestones may thus guide effective intervention for health professionals, parents and other professionals. Also summarized and explored are the role of speech perception and production, grammatical and syntactic skills, and metacognitive skills, including phonological awareness. PMID:19030330

  13. The data acquisition system for SLD

    SciTech Connect

    Sherden, D.J.

    1987-04-01

    This paper describes the data acquisition system planned for the SLD detector, which is being constructed for use with the SLAC Linear Collider (SLC). Analog electronics, heavily incorporating hybrid and custom VLSI circuitry, is mounted on the detector itself. Extensive use is made of multiplexing through optical fibers to a FASTBUS readout system. The low repetition rate of the SLC allows a relatively simple software-based trigger. Hardware and software processors within the acquisition modules are used to reduce the large volume of data per event and to calibrate the electronics. A farm of microprocessors is used for full reconstruction of a sample of events prior to transmission to the host.

  14. Modulated Acquisition of Spatial Distortion Maps

    PubMed Central

    Volkov, Alexey; Gros, Jerneja Žganec; Žganec, Mario; Javornik, Tomaž; Švigelj, Aleš

    2013-01-01

    This work discusses a novel approach to image acquisition which improves the robustness of captured data required for 3D range measurements. By applying a pseudo-random code modulation to sequential acquisition of projected patterns the impact of environmental factors such as ambient light and mutual interference is significantly reduced. The proposed concept has been proven with an experimental range sensor based on the laser triangulation principle. The proposed design can potentially enhance the use of this principle to a variety of outdoor applications, such as autonomous vehicles, pedestrians' safety, collision avoidance, and many other tasks, where robust real-time distance detection in real world environment is crucial. PMID:23966196

  15. Strategic petroleum reserve data acquisition system

    SciTech Connect

    Merillat, P D; Bauer, A G

    1980-10-01

    The Strategic Petroleum Reserve Data Acquisition System is a general purpose, digital data acquisition system designed for field use in the DOE's Strategic Petroleum Reserve testing and monitoring program. The system is computer driven, under the control of an operator. The system is designed to allow the operator to perform pre-test system configuration; test monitoring and control; and post test analysis. This document is a system description and an operator users manual. Topics covered include: configuration and running on-line tests, software documentation, and maintenance programming information.

  16. Interactive specification acquisition via scenarios: A proposal

    NASA Technical Reports Server (NTRS)

    Hall, Robert J.

    1992-01-01

    Some reactive systems are most naturally specified by giving large collections of behavior scenarios. These collections not only specify the behavior of the system, but also provide good test suites for validating the implemented system. Due to the complexity of the systems and the number of scenarios, however, it appears that automated assistance is necessary to make this software development process workable. Interactive Specification Acquisition Tool (ISAT) is a proposed interactive system for supporting the acquisition and maintenance of a formal system specification from scenarios, as well as automatic synthesis of control code and automated test generation. This paper discusses the background, motivation, proposed functions, and implementation status of ISAT.

  17. CO2 Acquisition Membrane (CAM) Project

    NASA Technical Reports Server (NTRS)

    Mason, L. W.; Way, J. D.; Vlasse, M.

    2001-01-01

    The CO2 Acquisition Membrane (CAM) project will develop, test, and analyze membrane materials for separation and purification of carbon dioxide (CO2) from mixtures of gases, such as those found in the Martian atmosphere. The CAM technology will enable passive separation of these gases, allow energy efficient acquisition and purification of these important resources, and lay the foundation for future unmanned sample return and human space missions. The CAM membranes are targeted toward In Situ Resource Utilization (ISRU) applications, such as In Situ Propellant Production (ISPP) and In Situ Consumables Production (ISCP).

  18. Acquisition and Role of Molybdate in Pseudomonas aeruginosa

    PubMed Central

    Pederick, Victoria G.; Eijkelkamp, Bart A.; Ween, Miranda P.; Begg, Stephanie L.; Paton, James C.

    2014-01-01

    In microaerophilic or anaerobic environments, Pseudomonas aeruginosa utilizes nitrate reduction for energy production, a process dependent on the availability of the oxyanionic form of molybdenum, molybdate (MoO42−). Here, we show that molybdate acquisition in P. aeruginosa occurs via a high-affinity ATP-binding cassette permease (ModABC). ModA is a cluster D-III solute binding protein capable of interacting with molybdate or tungstate oxyanions. Deletion of the modA gene reduces cellular molybdate concentrations and results in inhibition of anaerobic growth and nitrate reduction. Further, we show that conditions that permit nitrate reduction also cause inhibition of biofilm formation and an alteration in fatty acid composition of P. aeruginosa. Collectively, these data highlight the importance of molybdate for anaerobic growth of P. aeruginosa and reveal novel consequences of nitrate reduction on biofilm formation and cell membrane composition. PMID:25172858

  19. Acquisition and role of molybdate in Pseudomonas aeruginosa.

    PubMed

    Pederick, Victoria G; Eijkelkamp, Bart A; Ween, Miranda P; Begg, Stephanie L; Paton, James C; McDevitt, Christopher A

    2014-11-01

    In microaerophilic or anaerobic environments, Pseudomonas aeruginosa utilizes nitrate reduction for energy production, a process dependent on the availability of the oxyanionic form of molybdenum, molybdate (MoO4 (2-)). Here, we show that molybdate acquisition in P. aeruginosa occurs via a high-affinity ATP-binding cassette permease (ModABC). ModA is a cluster D-III solute binding protein capable of interacting with molybdate or tungstate oxyanions. Deletion of the modA gene reduces cellular molybdate concentrations and results in inhibition of anaerobic growth and nitrate reduction. Further, we show that conditions that permit nitrate reduction also cause inhibition of biofilm formation and an alteration in fatty acid composition of P. aeruginosa. Collectively, these data highlight the importance of molybdate for anaerobic growth of P. aeruginosa and reveal novel consequences of nitrate reduction on biofilm formation and cell membrane composition. PMID:25172858

  20. Cholinergic hypofunction impairs memory acquisition possibly through hippocampal Arc and BDNF downregulation.

    PubMed

    Gil-Bea, Francisco J; Solas, Maite; Mateos, Laura; Winblad, Bengt; Ramírez, María J; Cedazo-Mínguez, Angel

    2011-09-01

    Recent evidence suggests that activity-regulated cytoskeleton associated protein (Arc) and brain-derived neurotrophic factor (BDNF) are key players in the cellular mechanisms that trigger synaptic changes and memory consolidation. Cholinergic deafferentiation of hippocampus has been largely shown to induce memory impairments in different behavioral tasks. However, the mechanisms underlying cholinergic-induced memory formation remain unclear. The role of hippocampal cholinergic denervation on synaptic consolidation and further acquisition of spatial memory was hereby examined by analyzing Arc and BDNF in standard environment and after behavioral training in Morris water maze (MWM). In standard environment, a cholinergic hypofunction induced by the toxin (192) IgG-saporin led to significant decreases in Arc protein and mRNA as well as in BDNF. Lesioned rats subjected to MWM showed a worse acquisition performance that was reversed after galantamine treatment. Recovery of memory acquisition was accompanied by normalization of Arc and BDNF levels in hippocampus. Stimulation of muscarinic, but not nicotinic receptors, in hippocampal primary neurons caused a rapid induction of Arc production. These data suggest that cholinergic denervation of hippocampus leads to deficits in muscarinic-dependent induction of Arc and a subsequent impairment of spatial memory acquisition. PMID:20865740

  1. The Spirituality of Second Language Acquisition

    ERIC Educational Resources Information Center

    Jackson, Baxter

    2006-01-01

    Parallels between the reconstruction of self in Alcoholics Anonymous and the reconstruction of self in second language acquisition are drawn out and examined in three areas: ego deflation, identification at depth, and mutual assistance. These spiritual principles are shown to be theoretically and empirically supported in SLA literature and…

  2. Cerebral Dominance, Language Acquisition, and Foreign Accents.

    ERIC Educational Resources Information Center

    Scovel, Tom

    1969-01-01

    Implicit in the discussion of views taken by Wolfe, Geschwind, and Newmark is a claim that no learning theory based solely on "nurture" can account for the fact that language acquisition in childhood is a trait, in adulthood a skill. The child can master the language system completely, regardless of his intellectual capacity or his social…

  3. Ultrasonic Scanner Control and Data Acquisition

    NASA Technical Reports Server (NTRS)

    Hemann, John

    2002-01-01

    The research accomplishments under this grant were very extensive in the areas of ULTRASONIC SCANNER CONTROL AND DATA ACQUISITION. Rather than try to summarize all this research I have enclosed research papers and reports which were completed with the hnding provided by the grant. These papers and reports are listed below:

  4. 16 CFR 801.4 - Secondary acquisitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... holds more than $50 million (as adjusted) of the voting securities of corporation X (but does not control X), and “A” and “X” satisfy Sections 7A (a)(1) and (a)(2), “A” must file notification separately with respect to its secondary acquisition of voting securities of X. “X” must file notification...

  5. 16 CFR 801.4 - Secondary acquisitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... holds more than $50 million (as adjusted) of the voting securities of corporation X (but does not control X), and “A” and “X” satisfy Sections 7A (a)(1) and (a)(2), “A” must file notification separately with respect to its secondary acquisition of voting securities of X. “X” must file notification...

  6. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1990-01-01

    Archival reports on developments in programs managed by the JPL Office of Telecommunications and Data Acquisition (TDA) are provided. Topics covered include: DSN advanced systems (tracking and ground-based navigation; communications, spacecraft-ground; and station control and system technology) and DSN systems implementation (capabilities for existing projects; capabilities for new projects; TDA program management and analysis; and Goldstone solar system radar).

  7. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1989-01-01

    Archival reports on developments in programs managed by the Jet Propulsion Laboratory's Office of Telecommunications and Data Acquisition are provided. Space communications, radio navigation, radio science, and ground based radio and radio astronomy are discussed. Deep Space Network projects are also discussed.

  8. Basic Concept Acquisition in Learning Disabled Children.

    ERIC Educational Resources Information Center

    DiNapoli, Nicholas Paul; And Others

    The Boehm Test of Basic Concepts (BTBC) (Boehm, 1971) was administered to 99 children (ages 7-10) who had been diagnosed as learning disabled and attended special schools in the New York area. It was hypothesized that the learning disabled children would exhibit a delay in the acquisition of the basic concepts, but would display a similar order of…

  9. Acquisition Order of Coherence Relations in Turkish

    ERIC Educational Resources Information Center

    Demirgunes, Sercan

    2015-01-01

    Coherence as one of the criteria for textuality is the main element of a well-produced text. In the literature, there are many studies on the classification of coherence relations. Although there are different classifications on coherence relations, similar findings are reported regarding the acquisition order of coherence relations in different…

  10. Extended, Embodied Cognition and Second Language Acquisition

    ERIC Educational Resources Information Center

    Atkinson, Dwight

    2010-01-01

    A "cognitivist" approach to cognition has traditionally dominated second language acquisition (SLA) studies. In this article, I examine two alternative approaches--"extended cognition" and "embodied cognition"--for how they might help us conceptualize SLA. More specifically, I present: (i) summaries of extended and embodied cognition, followed by…

  11. Focused Communication Tasks and Second Language Acquisition.

    ERIC Educational Resources Information Center

    Nobuyoshi, Junko; Ellis, Rod

    1993-01-01

    A study of six adult learners of English as a Second Language provides some evidence to suggest that pushing learners to produce more accurate output contributes to acquisition. The data also demonstrate how this might be achieved through focused communication tasks. (Contains 11 references.) (Author)

  12. Grammaticality Judgments and Second Language Acquisition.

    ERIC Educational Resources Information Center

    Ellis, Rod

    1991-01-01

    A critical examination of grammaticality judgment tasks in second-language acquisition research focuses on the theoretical assumptions underlying such tasks and describes an investigation indicating that grammaticality judgment tasks elicit a particular kind of performance that needs to be better understood before being used as a basis for…

  13. Rad Chem data acquisition chassis users manual

    SciTech Connect

    Jones, B.A.

    1980-01-24

    The Shiva Laser at LLL requires many forms of diagnostics to measure and analyze fusion experiments. This manual describes the operation of a Micro-Processor controlled data acquisition system designed at LLL to measure Neutron Activation during fusion experiments on the Shiva Laser.

  14. NASA Data Acquisitions System (NDAS) Software Architecture

    NASA Technical Reports Server (NTRS)

    Davis, Dawn; Duncan, Michael; Franzl, Richard; Holladay, Wendy; Marshall, Peggi; Morris, Jon; Turowski, Mark

    2012-01-01

    The NDAS Software Project is for the development of common low speed data acquisition system software to support NASA's rocket propulsion testing facilities at John C. Stennis Space Center (SSC), White Sands Test Facility (WSTF), Plum Brook Station (PBS), and Marshall Space Flight Center (MSFC).

  15. Auditory Processing Disorder and Foreign Language Acquisition

    ERIC Educational Resources Information Center

    Veselovska, Ganna

    2015-01-01

    This article aims at exploring various strategies for coping with the auditory processing disorder in the light of foreign language acquisition. The techniques relevant to dealing with the auditory processing disorder can be attributed to environmental and compensatory approaches. The environmental one involves actions directed at creating a…

  16. A flexible modelling software for data acquisition

    SciTech Connect

    Shu, Y.; Chen, Y.; Yang, S.; Liu, G. )

    1992-04-01

    A flexible modeling software for pulsed data acquisition is based on an event-driven simulator. It can be used to simulate a wide variety of systems which can be modeled as open queuing networks. This paper also describes some modeling program techniques and applications in plasma physics laboratories.

  17. DATA ACQUISITION SYSTEM FOR RAPID KINETIC EXPERIMENTS

    EPA Science Inventory

    A data acquisition system has been developed to collect, analyze and store large volumes of rapid kinetic data measured from a stopped-flow spectrophotometer. A digital minicomputer, with an A/D converter, tape drive unit and formatter, analog recorder, oscilloscope, and input/ou...

  18. Processability Theory and German Case Acquisition

    ERIC Educational Resources Information Center

    Baten, Kristof

    2011-01-01

    This article represents the first attempt to formulate a hypothetical sequence for German case acquisition by Dutch-speaking learners on the basis of Processability Theory (PT). It will be argued that case forms emerge corresponding to a development from lexical over phrasal to interphrasal morphemes. This development, however, is subject to a…

  19. 75 FR 10567 - Commerce Acquisition Regulation (CAR)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... proposed rule published on October 13, 2009 in the Federal Register (74 FR 52541). The document is also... Commerce 48 CFR Chapter 13 Commerce Acquisition Regulation (CAR); Final Rule #0;#0;Federal Register / Vol. 75, No. 44 / Monday, March 8, 2010 / Rules and Regulations#0;#0; ] DEPARTMENT OF COMMERCE 48...

  20. Can Communicative Principles Enhance Classical Language Acquisition?

    ERIC Educational Resources Information Center

    Overland, Paul; Fields, Lee; Noonan, Jennifer

    2011-01-01

    Is it feasible for nonfluent instructors to teach Biblical Hebrew by communicative principles? If it is feasible, will communicative instruction enhance postsecondary learning of a classical language? To begin answering these questions, two consultants representing second language acquisition (SLA) and technology-assisted language learning led 8…

  1. Three Myths from the Language Acquisition Literature

    ERIC Educational Resources Information Center

    Schoneberger, Ted

    2010-01-01

    Three popular assertions have hindered the promotion of an empiricist approach to language acquisition: (a) that Brown and Hanlon (1970) claimed to offer data that parents do not reinforce their children's grammaticality; (b) that Brown and Hanlon also claimed to offer data that parents do not provide negative evidence (i.e., corrective feedback)…

  2. Motor Acquisition Rate in Brazilian Infants

    ERIC Educational Resources Information Center

    Lopes, Virlaine Bardella; de Lima, Carolina Daniel; Tudella, Eloisa

    2009-01-01

    This study used the Alberta Infant Motor Scale (AIMS) with the aim of characterizing motor acquisition rate in 70 healthy 0-6-month-old Brazilian infants, as well as comparing both emergence (initial age) and establishment (final age) of each skill between the study sample and the AIMS normative data. New motor skills were continuously acquired…

  3. Facilitation and Practice in Verb Acquisition

    ERIC Educational Resources Information Center

    Keren-Portnoy, Tamar

    2006-01-01

    This paper presents a model of syntax acquisition, whose main points are as follows: Syntax is acquired in an item-based manner; early learning facilitates subsequent learning--as evidenced by the accelerating rate of new verbs entering a given structure; and mastery of syntactic knowledge is typically achieved through practice--as evidenced by…

  4. Precursors to Onset Clusters in Acquisition

    ERIC Educational Resources Information Center

    Gierut, Judith A.; O'Connor, Kathleen M.

    2002-01-01

    Two lawful relationships involving word-initial onset clusters have been advanced in the acquisition literature; namely, that clusters imply affricates, and that liquid clusters imply a liquid distinction. This study evaluated and extended the validity of these implicational laws in a population of 110 children (aged 3;0 to 8;6) with functional…

  5. Phonological Acquisition: Recent Attainments and New Challenges

    ERIC Educational Resources Information Center

    Peperkamp, Sharon

    2003-01-01

    Infants' phonological acquisition during the first 18 months of life has been studied within experimental psychology for some 30 years. Current research themes include statistical learning mechanisms, early lexical development, and models of phonetic category perception. So far, linguistic theories have hardly been taken into account. These…

  6. The Reading Venture: Accelerating Language Acquisition.

    ERIC Educational Resources Information Center

    Sifontes, Aida I.; Baez, Dodie

    This presentation describes how to use reading to improve second language acquisition. Part 1, "Building Awareness of Reading Habits and Attitudes," has students report their habits and attitudes about reading in English and their native language and recognize the importance of reading for improving English skills. Part 2, "Choosing a Book," has…

  7. Incidental Foreign Language Acquisition from Media Exposure

    ERIC Educational Resources Information Center

    Kuppens, An H.

    2010-01-01

    A number of experimental studies have demonstrated the incidental acquisition of a foreign language by children and adolescents when watching foreign language television. While such experiments can only establish short-term effects, this article investigates the extent to which children's foreign language skills benefit from their long-term…

  8. Virtue Acquisition: The Paradox of Striving

    ERIC Educational Resources Information Center

    Snow, Nancy

    2016-01-01

    Aristotelian-inspired accounts of virtue acquisition stress guided practice and habituated action to develop virtue. This emphasis on action can lead to the "paradox of striving". The paradox occurs when we try too hard to act well and thereby spoil our efforts. I identify four forms of striving--forcing, impulsivity, overthinking, and…

  9. 23 CFR 710.501 - Early acquisition.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... accordance with the provisions of 49 CFR part 24; (4) The State complied with the requirements of title VI of..., requires the STD to demonstrate that: (1) Prior to acquisition, the STD made the certifications and determinations required by 23 U.S.C. 108(c)(2)(C) and (D); and (2) The STD obtained concurrence from...

  10. 23 CFR 710.501 - Early acquisition.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... accordance with the provisions of 49 CFR part 24; (4) The State complied with the requirements of title VI of..., requires the STD to demonstrate that: (1) Prior to acquisition, the STD made the certifications and determinations required by 23 U.S.C. 108(c)(2)(C) and (D); and (2) The STD obtained concurrence from...

  11. 23 CFR 710.501 - Early acquisition.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... accordance with the provisions of 49 CFR part 24; (4) The State complied with the requirements of title VI of..., requires the STD to demonstrate that: (1) Prior to acquisition, the STD made the certifications and determinations required by 23 U.S.C. 108(c)(2)(C) and (D); and (2) The STD obtained concurrence from...

  12. 23 CFR 710.501 - Early acquisition.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... accordance with the provisions of 49 CFR part 24; (4) The State complied with the requirements of title VI of..., requires the STD to demonstrate that: (1) Prior to acquisition, the STD made the certifications and determinations required by 23 U.S.C. 108(c)(2)(C) and (D); and (2) The STD obtained concurrence from...

  13. 23 CFR 710.501 - Early acquisition.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... accordance with the provisions of 49 CFR part 24; (4) The State complied with the requirements of title VI of..., requires the STD to demonstrate that: (1) Prior to acquisition, the STD made the certifications and determinations required by 23 U.S.C. 108(c)(2)(C) and (D); and (2) The STD obtained concurrence from...

  14. An Emergentist Perspective on Heritage Language Acquisition

    ERIC Educational Resources Information Center

    O'Grady, William; Kwak, Hye-Young; Lee, On-Soon; Lee, Miseon

    2011-01-01

    It is widely recognized that the processor has a key role to play in creating and strengthening the mapping between form and meaning that is integral to language use. Adopting an emergentist approach to heritage language acquisition, the current study considers the extent to which the operation of the processor can contribute to an account of what…

  15. Precision cryogenic temperature data acquisition system

    NASA Astrophysics Data System (ADS)

    Farah, Y.; Sondericker, J. H.

    1985-08-01

    A Multiplexed Temperature Data Acquisition System with an overall precision of + or - 25 ppM has been designed using state-of-the-art electronics to accurately read temperature between 2.4 K and 600 K from pre-calibrated transducers such as germanium, silicon diode, thermistor or platinum temperature sensors.

  16. Making practice retirement acquisition work for you.

    PubMed

    Holdren, R C

    1992-01-01

    Author Richard Holdren writes that medical practice acquisition can have a dramatic impact on hospital and group practice revenue in a "zero-sum game," where both parties are competing for a shrinking patient and dollar base. Holdren describes how such a program is built on the concepts of support management and "grow your own." PMID:10116646

  17. 48 CFR 970.2301 - Sustainable acquisition.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    .... 970.2301 Section 970.2301 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Environment, Energy and Water Efficiency, Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2301 Sustainable...

  18. Sleep quality influences subsequent motor skill acquisition.

    PubMed

    Appleman, Erica R; Albouy, Genevieve; Doyon, Julien; Cronin-Golomb, Alice; King, Bradley R

    2016-06-01

    While the influence of sleep on motor memory consolidation has been extensively investigated, its relation to initial skill acquisition is less well understood. The purpose of the present study was to investigate the influence of sleep quality and quantity on subsequent motor skill acquisition in young adults without sleep disorders. Fifty-five healthy adults (mean age = 23.8 years; 34 women) wore actigraph wristbands for 4 nights, which provided data on sleep patterns before the experiment, and then returned to the laboratory to engage in a motor sequence learning task (explicit 5-item finger sequence tapping task). Indicators of sleep quality and quantity were then regressed on a measure of motor skill acquisition (Gains Within Training, GWT). Wake After Sleep Onset (WASO; i.e., the total amount of time the participants spent awake after falling asleep) was significantly and negatively related to GWT. This effect was not because of general arousal level, which was measured immediately before the motor task. Conversely, there was no relationship between GWT and sleep duration or self-reported sleep quality. These results indicate that sleep quality, as assessed by WASO and objectively measured with actigraphy before the motor task, significantly impacts motor skill acquisition in young healthy adults without sleep disorders. (PsycINFO Database Record PMID:26881313

  19. NASA's acquisition requirements for configuration management

    NASA Technical Reports Server (NTRS)

    Coletta, Mark P.

    1992-01-01

    A viewgraph presentation on NASA's acquisition requirements for configuration management (CM) goes over CM requirements for single mission and multi-mission orientations, CM automation and CALS implementation initiatives, NASA implementation of DOD standards and DID's (data item descriptions), impact of traceability in NASA CM support, NASA's CM efforts in modifying/upgrading equipment, and CM control of multi-vendor hardware.

  20. Effects of Multimedia Annotations on Vocabulary Acquisition.

    ERIC Educational Resources Information Center

    Chun, Dorothy M.; Plass, Jan L.

    1996-01-01

    Discusses studies of German students using "CyberBuch," a hypermedia application for reading German texts that contains annotations for words in the form of text, pictures, and video. The article examines incidental vocabulary learning, the effectiveness of different types of annotations for vocabulary acquisition, and the effect of look-up…

  1. The Acquisition of Visual Information. Final Report.

    ERIC Educational Resources Information Center

    Craig, Eugene A.

    Two important processes in the acquisition of visually presented information are the ability to maintain discrete perceptual events as separate in time and the ability to abstract information from brief exposures. Five phenomena were measured which appear to reflect these abilities. Critical Flicker Frequency (CFF) is the intermittency rate at…

  2. 77 FR 39724 - Land Acquisitions: 19 Pueblos

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... Bureau of Indian Affairs Land Acquisitions: 19 Pueblos AGENCY: Bureau of Indian Affairs, Interior. ACTION: Notice of final agency determination to take land into trust. SUMMARY: The Assistant Secretary--Indian Affairs made a final agency determination to acquire approximately 8.43 acres of land into trust for...

  3. Complexity and Conflicting Grammars in Language Acquisition

    ERIC Educational Resources Information Center

    Westergaard, Marit

    2014-01-01

    The article by Amaral and Roeper (this issue; henceforth A&R) presents many interesting ideas about first and second language acquisition as well as some experimental data convincingly illustrating the difference between production and comprehension. The article extends the concept of Universal Bilingualism proposed in Roeper (1999) to second…

  4. On Teaching Strategies in Second Language Acquisition

    ERIC Educational Resources Information Center

    Yang, Hong

    2008-01-01

    How to acquire a second language is a question of obvious importance to teachers and language learners, and how to teach a second language has also become a matter of concern to the linguists' interest in the nature of primary linguistic data. Starting with the development stages of second language acquisition and Stephen Krashen's theory, this…

  5. Color Vision Deficits and Literacy Acquisition.

    ERIC Educational Resources Information Center

    Hurley, Sandra Rollins

    1994-01-01

    Shows that color blindness, whether partial or total, inhibits literacy acquisition. Offers a case study of a third grader with impaired color vision. Presents a review of literature on the topic. Notes that people with color vision deficits are often unaware of the handicap. (RS)

  6. Directionality in Linguistic Change and Acquisition.

    ERIC Educational Resources Information Center

    Gomes, Christina Abreu

    1999-01-01

    Focuses on the directionality observed in the processes of change and acquisition of the prepositions that replaced Latin cases in the speech of Rio de Janeiro and in the contact Portuguese spoken by Brazilian Indians in the region of Xingu. (Author/VWL)

  7. Early Spelling Acquisition: Writing Beats the Computer.

    ERIC Educational Resources Information Center

    Cunningham, Anne E.; Stanovich, Keith E.

    1990-01-01

    The spelling performance of 22 male and 26 female first graders using a simultaneous oral spelling method was compared when students were writing the words, spelling them with letter tiles, or typing them on a computer keyboard. Results of two experiments indicate the superiority of handwriting in improving spelling acquisition. (SLD)

  8. Extensive Reading in Enhancing Lexical Chunks Acquisition

    ERIC Educational Resources Information Center

    Pereyra, Nilsa

    2015-01-01

    The purpose of this action research was to investigate the effect of extensive reading and related activities on the acquisition of lexical chunks in EFL students. Seven adult EFL learners with an Intermediate level volunteered to take part in the 16 week project following Extensive Reading principles combined with tasks based on the Lexical…

  9. Knowledge acquisition for a simple expert controller

    NASA Technical Reports Server (NTRS)

    Bieker, B.

    1987-01-01

    A method is presented for process control which has the properties of being incremental, cyclic and top-down. It is described on the basis of the development of an expert controller for a simple, but nonlinear control route. A quality comparison between expert controller and process operator shows the ability of the method for knowledge acquisition.

  10. A Connectionist Approach to Language Acquisition.

    ERIC Educational Resources Information Center

    Lizardi, Luis O.

    This paper attempts to synthesize how biological-nativist theories emerged as a response to logical and empirical flaws in behaviorist learning theories, and how in turn, recent research findings in Developmental Cognitive Neuroscience and Connectionist models of language acquisition are questioning the present innatist framework. As a result of…

  11. Guidelines for dynamic data acquisition and analysis

    NASA Technical Reports Server (NTRS)

    Piersol, Allan G.

    1992-01-01

    The recommendations concerning pyroshock data presented in the final draft of a proposed military handbook on Guidelines for Dynamic Data Acquisition and Analysis are reviewed. The structural responses produced by pyroshocks are considered to be one of the most difficult types of dynamic data to accurately measure and analyze.

  12. Guidelines for dynamic data acquisition and analysis

    NASA Astrophysics Data System (ADS)

    Piersol, Allan G.

    1992-10-01

    The recommendations concerning pyroshock data presented in the final draft of a proposed military handbook on Guidelines for Dynamic Data Acquisition and Analysis are reviewed. The structural responses produced by pyroshocks are considered to be one of the most difficult types of dynamic data to accurately measure and analyze.

  13. Data acquisition for a large neutrino detector

    SciTech Connect

    Ahrens, L.A.; Aronson, S.A.; Connolly, P.L.

    1983-01-01

    A hierarchical, distributed intelligence data acquisition system which has been used for the past two years in neutrino experiments at Brookhaven National Laboratory is described. Performance characteristics and the nature of problems encountered in bringing the system to maturity are discussed and some generalizations of the experience are suggested.

  14. Adaptive line enhancers for fast acquisition

    NASA Technical Reports Server (NTRS)

    Yeh, H.-G.; Nguyen, T. M.

    1994-01-01

    Three adaptive line enhancer (ALE) algorithms and architectures - namely, conventional ALE, ALE with double filtering, and ALE with coherent accumulation - are investigated for fast carrier acquisition in the time domain. The advantages of these algorithms are their simplicity, flexibility, robustness, and applicability to general situations including the Earth-to-space uplink carrier acquisition and tracking of the spacecraft. In the acquisition mode, these algorithms act as bandpass filters; hence, the carrier-to-noise ratio (CNR) is improved for fast acquisition. In the tracking mode, these algorithms simply act as lowpass filters to improve signal-to-noise ratio; hence, better tracking performance is obtained. It is not necessary to have a priori knowledge of the received signal parameters, such as CNR, Doppler, and carrier sweeping rate. The implementation of these algorithms is in the time domain (as opposed to the frequency domain, such as the fast Fourier transform (FFT)). The carrier frequency estimation can be updated in real time at each time sample (as opposed to the batch processing of the FFT). The carrier frequency to be acquired can be time varying, and the noise can be non-Gaussian, nonstationary, and colored.

  15. Inexpensive Data Acquisition with a Sound Card

    ERIC Educational Resources Information Center

    Hassan, Umer; Pervaiz, Saad; Anwar, Muhammad Sabieh

    2011-01-01

    Signal generators, oscilloscopes, and data acquisition (DAQ) systems are standard components of the modern experimental physics laboratory. The sound card, a built-in component in the ubiquitous personal computer, can be utilized for all three of these tasks and offers an attractive option for labs in developing countries such as…

  16. Reading and Spelling Acquisition in Thai Children

    ERIC Educational Resources Information Center

    Winskel, Heather; Iemwanthong, Kanyarat

    2010-01-01

    Thai, a tonal language, has its own distinctive alphabetic orthography. The study investigates reading and spelling development in Thai children, with an aim of examining the grain size that is predominantly used when reading and spelling. Furthermore, word and nonword lists were developed to examine the acquisition of the complex system of vowels…

  17. Information Acquisition in Children Undergoing Medical Procedures.

    ERIC Educational Resources Information Center

    Melamed, Barbara G.; And Others

    In order to evaluate how individual characteristics of hospitalized children influence their acquisition of information presented in a film depicting preparation for surgery, 42 children between the ages of 4 to 17 were assigned to experimental and control groups that were matched for age, sex, race, type of surgery and previous hospital…

  18. Psycholinguistic Techniques in Second Language Acquisition Research.

    ERIC Educational Resources Information Center

    Marinis, Theodore

    2003-01-01

    Presents the benefits of using online methodologies in second language acquisition (SLA) research. Provides a selection of online experiments that have been widely used in first and second language processing studies that are suitable for SLA research and discusses the hardware and software packages required for setting up a psycholinguistic…

  19. Collection Development/Acquisitions: Collaboration or Conflict?

    ERIC Educational Resources Information Center

    Clement, Mercedes

    At the Daytona Beach Community College (DBCC) Library, collection development, selection, and acquisitions function as levels of a hierarchy. The highest level is collection development, which constitutes the planning function. Meetings are held annually to assess user needs, evaluate the present collection, discuss budget considerations, plan for…

  20. Rethinking the Acquisition of Kinship Terms.

    ERIC Educational Resources Information Center

    Hirschfeld, Lawrence A.

    1989-01-01

    Reconsiders the predominant views on the acquisition of kinship terms. Topics discussed include: (1) kinship and the ontogenesis of logic; (2) kinship and semantic development; (3) the language learner as naive theorist; (4) development and domain specificity; and (5) an alternative approach to kinship. (RJC)

  1. Using Questions To Facilitate Motor Skill Acquisition.

    ERIC Educational Resources Information Center

    Knight, G. William; And Others

    1997-01-01

    Describes a dental teaching strategy that promotes acquisition of psychomotor skills through use of metacognition and problem-solving. In five steps, questions are asked to guide the learner through a sequence of discriminations leading to recognition of problems and solutions. Clearly defined criteria in a sequence reflecting procedure are…

  2. The Acquisition of Japanese "Gender" Particles.

    ERIC Educational Resources Information Center

    Sakata, Minako

    1991-01-01

    A study of the acquisition of Japanese sentence-final gender particles in six two year olds, as evidenced in conversations between the children and their mothers, found that the children were sensitive to the gender differences encoded in the particles and strongly sensitive to the social information provided by their parents. (10 references) (MSE)

  3. The UG Paradox in L2 Acquisition.

    ERIC Educational Resources Information Center

    Clahsen, Harald; Muysken, Pieter

    1989-01-01

    Suggests that differences between first- and second-language learners are due to principles of universal grammar (UG) that guide first language (L1), but not second language (L2) acquisition. This view can be reconciled with the idea that L2 learners can use UG principles to some extent in evaluating target sentences. (49 references) (Author/VWL)

  4. Mergers and acquisitions: a most strategic decision.

    PubMed

    Laine, Mike; Tyler, David

    2007-11-01

    Five steps are basic to successfully concluding (or deciding not to conclude) a merger or acquisition: Identify targets. Begin with the end in mind. Choose a transaction team. Perform due diligence. Commit to the deal--or to no deal. PMID:18018794

  5. 2 CFR 200.2 - Acquisition cost.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false Acquisition cost. 200.2 Section 200.2 Grants and Agreements Office of Management and Budget Guidance for Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GUIDANCE Reserved UNIFORM ADMINISTRATIVE REQUIREMENTS, COST PRINCIPLES, AND...

  6. Acquisition speed comparison of microscope software programs.

    PubMed

    Biehlmaier, Oliver; Hehl, Joachim; Csucs, Gabor

    2011-06-01

    Reliable software is a prerequisite for successful operation of a modern wide field fluorescence microscope. When used for live cell imaging, acquisition speed is of particular interest. This is both because biological processes can be highly-dynamic, and to avoid unnecessary photobleaching and phototoxicity of living samples. This article shows that besides the hardware (microscope) components themselves, the acquisition control software is an important influencing factor of speed performance. We tested and compared the speed performance of five different generic applications (Image-Pro Plus, MetaMorph, Micro-Manager, SlideBook, and Volocity) using typical experimental setups involving a single specific state-of-the-art fluorescence microscope configuration. The test measurements included multichannel experiments, z-stacking, burst acquisition, as well as combinations of these measurements with time-lapse acquisitions. The measured data provided values for guiding the testing and analysis of other microscope systems with similar configurations. Despite the identical hardware settings, significant and surprisingly large speed differences were evident among the various software applications. Additionally, no application was identifiable as the fastest in all tests. Our work pinpoints the importance of the control software in determining a system's "real" maximal imaging speed. The study could serve as basis for further tests, eventually influencing the system selection criteria for speed-sensitive applications. PMID:21604324

  7. Shooting direction and crosswell seismic data acquisition

    SciTech Connect

    Liner, C.L.; Bozkurt, G.; Cox, V.D.

    1996-09-01

    Four crosswell seismic surveys were acquired in the Glenn Pool Field of northeastern Oklahoma as part of a multidisciplinary reservoir characterization project. The acquisition goal was to generate data suitable for tomographic traveltime inversion. Acquisition parameters and shooting geometry were selected by conducting a parameter test at the site. Following the parameter test, the first survey resulted in high quality data showing clear first arrivals, low ambient noise, some reflection events, and strong source-generated tube waves. The second survey involved a different receiver well and encountered high ambient noise levels. The noise was strong enough to prohibit first-arrival picking for much of the data. On-site analysis of the second survey revealed tube waves emanating from a perforated interval in the receiver well. This well was shut in and was not flowing fluid or gas at the surface. They interpret the source of ambient tube waves as borehole-to-formation fluid flow (circulation) associated with the perforations. Since this image plane was important for characterization of the reservoir, the survey was reshot (third survey) by reversing sources and receivers in the two wells. The resulting high-quality data indicates that shooting direction can be an important factor in crosswell seismic acquisition. This experience influenced acquisition of a previously planned fourth survey so that the ambient noise problem would be avoided.

  8. Neuroimaging and Research into Second Language Acquisition

    ERIC Educational Resources Information Center

    Sabourin, Laura

    2009-01-01

    Neuroimaging techniques are becoming not only more and more sophisticated but are also coming to be increasingly accessible to researchers. One thing that one should take note of is the potential of neuroimaging research within second language acquisition (SLA) to contribute to issues pertaining to the plasticity of the adult brain and to general…

  9. Values Acquisition: Some Critical Distinctions and Implications.

    ERIC Educational Resources Information Center

    Herman, William E.

    1997-01-01

    Examines critical issues in values education. Outlines the bipolar distinctions between the developmental and transmission theories of values acquisition and describes an integrative model that incorporates both approaches. Offers suggestions for parents, counselors, schools, and society based upon a process-and-product analysis of a…

  10. 32 CFR 21.605 - Acquisition.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 1 2012-07-01 2012-07-01 false Acquisition. 21.605 Section 21.605 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DoD GRANT AND AGREEMENT REGULATIONS DoD... more detailed definition at 48 CFR 2.101). In accordance with 31 U.S.C. 6303, procurement contracts...

  11. 32 CFR 21.605 - Acquisition.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 1 2013-07-01 2013-07-01 false Acquisition. 21.605 Section 21.605 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE DoD GRANT AND AGREEMENT REGULATIONS DoD... more detailed definition at 48 CFR 2.101). In accordance with 31 U.S.C. 6303, procurement contracts...

  12. Second Language Acquisition Theory and Pedagogy.

    ERIC Educational Resources Information Center

    Eckman, Fred R., Ed.; And Others

    Selected papers on second language acquisition and instruction from the University of Wisconsin at Madison symposium include the following: "Learning and Teaching: The Necessary Intersection" (Susan M. Gass); "Reenvisioning the Second Language Classroom: A Vygotskian Approach" (Linda Schinke-Llano); "The FOCAL SKILLS Approach: An Assessment"…

  13. Grapheme-Phoneme Acquisition of Deaf Preschoolers

    ERIC Educational Resources Information Center

    Beal-Alvarez, Jennifer S.; Lederberg, Amy R.; Easterbrooks, Susan R.

    2012-01-01

    We examined acquisition of grapheme-phoneme correspondences by 4 deaf and hard-of-hearing preschoolers using instruction from a curriculum designed specifically for this population supplemented by Visual Phonics. Learning was documented through a multiple baseline across content design as well as descriptive analyses. Preschoolers who used sign…

  14. Controls and data acquisition on Atlas

    SciTech Connect

    Scudder, D.W.; Hosack, K.W.; Parsons, W.M.; Reass, W.A.; Thompson, M.C.; Wysocki, F.J.; Creager, J.

    1997-09-01

    The control and data acquisition systems for Atlas will use a large degree of decentralization. By distributing control points close to the systems being controlled, the authors expect to simplify the task of isolating electronic systems from the large expected EMI pulses, allow connection of the various parts of the system by high-level fiber-optic networks, allow a simple configuration of the control and data acquisition screen rooms, and simplify the software efforts through the resulting modularization. The Atlas control system must control capacitor charging, machine and diagnostic timing and triggering, marx module diagnostics, vacuum systems, gas handling for railgaps, safety interlocks, and oil handling. Many of these tasks will be performed by industrial-style programmable logic controllers (PLCs). Each of 38 Marx bank maintenance units will have a control and diagnostic package which will monitor both charging and discharging current and railgap trigger timing. An unusual feature of digitizers to record each Marx module`s output waveform, plus nanosecond resolution time interval meters to record the firing time of each railgap. The machine data acquisition system for Atlas will be built around an SQL database, use National Instruments LabVIEW software to control data acquisition instruments and provide links for a variety of experimentalists` data analysis packages. World Wide Web access will provide an interface through which users can monitor experimental data and machine status.

  15. The Acquisition of Evidentiality and Source Monitoring

    ERIC Educational Resources Information Center

    Ozturk, Ozge; Papafragou, Anna

    2016-01-01

    Evidentiality in language marks how information contained in a sentence was acquired. For instance, Turkish has two past-tense morphemes that mark whether access to information was direct (typically, perception) or indirect (hearsay/inference). Full acquisition of evidential systems appears to be a late achievement cross-linguistically. Currently,…

  16. Modeling the Distinct Phases of Skill Acquisition

    ERIC Educational Resources Information Center

    Tenison, Caitlin; Anderson, John R.

    2016-01-01

    A focus of early mathematics education is to build fluency through practice. Several models of skill acquisition have sought to explain the increase in fluency because of practice by modeling both the learning mechanisms driving this speedup and the changes in cognitive processes involved in executing the skill (such as transitioning from…

  17. Foreign Language Acquisition and Melody Singing.

    ERIC Educational Resources Information Center

    Mora, Carmen Fonseca

    2000-01-01

    Considers the value of relating music and language in the English-as-Foreign-Language (EFL) classroom. This "melodic" approach is based on evidence that musicality of speech has an effect not only on the pronunciation skills of EFL students but also their entire acquisition process. (Author/VWL)

  18. Acquisitions List No. 41, January 1972.

    ERIC Educational Resources Information Center

    Planned Parenthood--World Population, New York, NY. Katherine Dexter McCormick Library.

    The Acquisitions List of demographic books and articles is issued every two months by the Katherine Dexter-McCormick Library. Divided into two parts, the first contains a list of books most recently acquired by the Library, each one annotated and also marked with the Library call number. The second part consists of a list of annotated articles,…

  19. Acquisitions List No. 39, October 1971.

    ERIC Educational Resources Information Center

    Planned Parenthood--World Population, New York, NY. Katherine Dexter McCormick Library.

    The Acquisitions List of demographic books and articles is issued every two months by the Katharine Dexter McCormick Library. Divided into two parts, the first contains a list of books most recently acquired by the Library, each one annotated and also marked with the Library call number. The second part consists of a list of annotated articles,…

  20. Magnetic Field Response Measurement Acquisition System

    NASA Technical Reports Server (NTRS)

    Woodard, Stanley E.; Taylor,Bryant D.; Shams, Qamar A.; Fox, Robert L.

    2007-01-01

    This paper presents a measurement acquisition method that alleviates many shortcomings of traditional measurement systems. The shortcomings are a finite number of measurement channels, weight penalty associated with measurements, electrical arcing, wire degradations due to wear or chemical decay and the logistics needed to add new sensors. Wire degradation has resulted in aircraft fatalities and critical space launches being delayed. The key to this method is the use of sensors designed as passive inductor-capacitor circuits that produce magnetic field responses. The response attributes correspond to states of physical properties for which the sensors measure. Power is wirelessly provided to the sensing element by using Faraday induction. A radio frequency antenna produces a time-varying magnetic field used to power the sensor and receive the magnetic field response of the sensor. An interrogation system for discerning changes in the sensor response frequency, resistance and amplitude has been developed and is presented herein. Multiple sensors can be interrogated using this method. The method eliminates the need for a data acquisition channel dedicated to each sensor. The method does not require the sensors to be near the acquisition hardware. Methods of developing magnetic field response sensors and the influence of key parameters on measurement acquisition are discussed. Examples of magnetic field response sensors and the respective measurement characterizations are presented. Implementation of this method on an aerospace system is discussed.

  1. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1989-01-01

    Developments in programs managed by JPL's Office of Telecommunications and Data Acquisition (TDA) are provided. Activities of the Deep Space Network and its associated Ground Communications Facility in planning, in supporting research and technology, in implementation, and in operations are reported in space communications, radio navigation, radio science, and ground-based radio and radar astronomy.

  2. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1985-01-01

    Developments in programs managed by the Jet Propulsion Laboratory's Office of Telecommunications and Data acquisition are discussed. Space communications, radio antennas, the Deep Space Network, antenna design, Project SETI, seismology, coding, very large scale integration, downlinking, and demodulation are among the topics covered.

  3. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1988-01-01

    This publication, one of a series formerly titled The Deep Space Network Progress Report, documents DSN progress in flight project support, tracking and data acquisition research and technology, network engineering, hardware and software implementation, and operations. In addition, developments in earth-based radio technology as applied to geodynamics, astrophysics, and the radio search for extraterrestrial intelligence are reported.

  4. The telecommunications and data acquisition report

    NASA Technical Reports Server (NTRS)

    Renzetti, N. A.

    1980-01-01

    Deep Space Network progress in flight project support, tracking and data acquisition research and technology, network engineering, hardware and software implemention, and operations is documented. In addition, developments in Earth based radio technology as applied to geodynamics, astrophysics, and the radio search for extraterrestrial intelligence are reported.

  5. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1983-01-01

    Archival reports on developments in programs managed by JPL's office of Telecommunications and Data Acquisition (TDA) are presented. In space communications, radio navigation, radio science, and ground-based radio astronomy, it reports on activities of the Deep Space Network (DSN) and its associated Ground Communications Facility (GCF) in planning, in supporting research and technology, in implementation, and in operations.

  6. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1989-01-01

    Archival reports on developments in programs managed by JPL's Office of Telecommunications and Data Acquisition (TDA) are presented. Activities of the Deep Space Network (DSN) and its associated Ground Communications Facility (GCF) related to DSN advanced systems, systems implementation, and DSN operations are addressed. In addition, recent developments in the NASA SETI (Search for Extraterrestrial Intelligence) sky survey are summarized.

  7. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1985-01-01

    Deep Space Network (DSN) progress in flight project support, tracking and data acquisition research and technology, network engineering, hardware and software implementation, and operation is discussed. In addition, developments in Earth-based radio technology as applied to geodynamics, astrophysics and the radio search for extraterrestrial intelligence are reported.

  8. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1987-01-01

    Developments in programs managed by the Jet Propulsion Laboratory's Office of Telecommunications and Data Acquisition are discussed. Topics discussed include sorption compression/mechanical expanded hybrid refrigeration, calculated 70-meter antenna performance for offset L-band, systolic arrays and stack decoding, and calibrations of Deep Space Network antennas.

  9. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1986-01-01

    Deep Space Network progress in flight project support, tracking and data acquisition research and technology, network engineering, hardware and software implementation, and operations is documented. In addition, developments in Earth-based radio technology as applied to geodynamics, astrophysics and the radio search for extraterrestrial intelligence are reported.

  10. The Telecommunications and Data Acquisition Report

    NASA Technical Reports Server (NTRS)

    Posner, E. C. (Editor)

    1986-01-01

    This publication, one of a series formerly titled The Deep Space Network (DSN) Progress Report, documents DSN progress in flight project support, tracking and data acquisition research and technology, network engineering, hardware and software implementation, and operations. In addition, developments in Earth-based radio technology as applied to geodynamics, astrophysics, and the radio search for extraterrestrial intelligence are reported.

  11. Organizational Newcomers' Acquisition of Information from Peers.

    ERIC Educational Resources Information Center

    Comer, Debra R.

    1991-01-01

    Studies the processes by which organizational newcomers acquire information from their peers. Indicates types of information acquired, channels through which they acquire it, and the relationship between type and channel. Suggests that information acquisition is affected by type of organization, newcomer-peer work interactions, and newcomer…

  12. Sociocultural Theory and Second Language Acquisition

    ERIC Educational Resources Information Center

    Lantolf, James P.; Beckett, Tracy G.

    2009-01-01

    Second language acquisition (SLA) research informed by sociocultural theory (henceforth, SCT) began in earnest with the publication of Frawley & Lantolf's (1985) article on L2 (second language) discourse (described in the timeline proper). Since then, well over 300 journal articles, book chapters and doctoral dissertations have appeared in the…

  13. Effective GPR Data Acquisition and Imaging

    NASA Astrophysics Data System (ADS)

    Sato, M.

    2014-12-01

    We have demonstrated that dense GPR data acquisition typically antenna step increment less than 1/10 wave length can provide clear 3-dimeantiona subsurface images, and we created 3DGPR images. Now we are interested in developing GPR survey methodologies which required less data acquisition time. In order to speed up the data acquisition, we are studying efficient antenna positioning for GPR survey and 3-D imaging algorithm. For example, we have developed a dual sensor "ALIS", which combines GPR with metal detector (Electromagnetic Induction sensor) for humanitarian demining, which acquires GPR data by hand scanning. ALIS is a pulse radar system, which has a frequency range 0.5-3GHz.The sensor position tracking system has accuracy about a few cm, and the data spacing is typically more than a few cm, but it can visualize the mines, which has a diameter about 8cm. 2 systems of ALIS have been deployed by Cambodian Mine Action Center (CMAC) in mine fields in Cambodia since 2009 and have detected more than 80 buried land mines. We are now developing signal processing for an array type GPR "Yakumo". Yakumo is a SFCW radar system which is a multi-static radar, consisted of 8 transmitter antennas and 8 receiver antennas. We have demonstrated that the multi-static data acquisition is not only effective in data acquisition, but at the same time, it can increase the quality of GPR images. Archaeological survey by Yakumo in large areas, which are more than 100m by 100m have been conducted, for promoting recovery from Tsunami attacked East Japan in March 2011. With a conventional GPR system, we are developing an interpolation method of radar signals, and demonstrated that it can increase the quality of the radar images, without increasing the data acquisition points. When we acquire one dimensional GPR profile along a survey line, we can acquire relatively high density data sets. However, when we need to relocate the data sets along a "virtual" survey line, for example a

  14. Iron acquisition from Pseudomonas aeruginosa siderophores by human phagocytes: an additional mechanism of host defense through iron sequestration?

    PubMed

    Britigan, B E; Rasmussen, G T; Olakanmi, O; Cox, C D

    2000-03-01

    Chelation of iron to iron-binding proteins is a strategy of host defense. Some pathogens counter this via the secretion of low-molecular-weight iron-chelating agents (siderophores). Human phagocytes possess a high-capacity mechanism for iron acquisition from low-molecular-weight iron chelates. Efficient acquisition and sequestration of iron bound to bacterial siderophores by host phagocytes could provide a secondary mechanism to limit microbial access to iron. In the present work we report that human neutrophils, macrophages, and myeloid cell lines can acquire iron from the two Pseudomonas aeruginosa siderophores. Analogous to iron acquisition from other low-molecular-weight chelates, iron acquisition from the siderophores is ATP independent, induced by multivalent cationic metals, and unaffected by inhibitors of endocytosis and pinocytosis. In vivo, this process could serve as an additional mechanism of host defense to limit iron availability to invading siderophore-producing microbes. PMID:10678937

  15. Iron Acquisition from Pseudomonas aeruginosa Siderophores by Human Phagocytes: an Additional Mechanism of Host Defense through Iron Sequestration?

    PubMed Central

    Britigan, Bradley E.; Rasmussen, George T.; Olakanmi, Oyebode; Cox, Charles D.

    2000-01-01

    Chelation of iron to iron-binding proteins is a strategy of host defense. Some pathogens counter this via the secretion of low-molecular-weight iron-chelating agents (siderophores). Human phagocytes possess a high-capacity mechanism for iron acquisition from low-molecular-weight iron chelates. Efficient acquisition and sequestration of iron bound to bacterial siderophores by host phagocytes could provide a secondary mechanism to limit microbial access to iron. In the present work we report that human neutrophils, macrophages, and myeloid cell lines can acquire iron from the two Pseudomonas aeruginosa siderophores. Analogous to iron acquisition from other low-molecular-weight chelates, iron acquisition from the siderophores is ATP independent, induced by multivalent cationic metals, and unaffected by inhibitors of endocytosis and pinocytosis. In vivo, this process could serve as an additional mechanism of host defense to limit iron availability to invading siderophore-producing microbes. PMID:10678937

  16. On Shaft Data Acquisition System (OSDAS)

    NASA Technical Reports Server (NTRS)

    Pedings, Marc; DeHart, Shawn; Formby, Jason; Naumann, Charles

    2012-01-01

    On Shaft Data Acquisition System (OSDAS) is a rugged, compact, multiple-channel data acquisition computer system that is designed to record data from instrumentation while operating under extreme rotational centrifugal or gravitational acceleration forces. This system, which was developed for the Heritage Fuel Air Turbine Test (HFATT) program, addresses the problem of recording multiple channels of high-sample-rate data on most any rotating test article by mounting the entire acquisition computer onboard with the turbine test article. With the limited availability of slip ring wires for power and communication, OSDAS utilizes its own resources to provide independent power and amplification for each instrument. Since OSDAS utilizes standard PC technology as well as shared code interfaces with the next-generation, real-time health monitoring system (SPARTAA Scalable Parallel Architecture for Real Time Analysis and Acquisition), this system could be expanded beyond its current capabilities, such as providing advanced health monitoring capabilities for the test article. High-conductor-count slip rings are expensive to purchase and maintain, yet only provide a limited number of conductors for routing instrumentation off the article and to a stationary data acquisition system. In addition to being limited to a small number of instruments, slip rings are prone to wear quickly, and introduce noise and other undesirable characteristics to the signal data. This led to the development of a system capable of recording high-density instrumentation, at high sample rates, on the test article itself, all while under extreme rotational stress. OSDAS is a fully functional PC-based system with 48 channels of 24-bit, high-sample-rate input channels, phase synchronized, with an onboard storage capacity of over 1/2-terabyte of solid-state storage. This recording system takes a novel approach to the problem of recording multiple channels of instrumentation, integrated with the test

  17. 78 FR 80381 - Federal Acquisition Regulation; Federal Acquisition Circular 2005-72; Small Entity Compliance Guide

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-31

    ... change, an interim rule which was published in the Federal Register at 78 FR 37686 on June 21, 2013. The... Acquisition Regulation (FAR). An asterisk (*) next to a rule indicates that a regulatory flexibility...

  18. 77 FR 39125 - Defense Acquisition Regulations System; Defense Federal Acquisition Regulation Supplement; Only...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-29

    ... at 76 FR 44293 on July 25, 2011, to address acquisitions using competitive procedures in which only..., 2011 (76 FR 59623) through October 7, 2011. DoD received comments on the proposed rule from...

  19. 76 FR 30362 - Federal Acquisition Regulation; Submission for OMB Review; Acquisition of Helium

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE... Regulation; Submission for OMB Review; Acquisition of Helium AGENCY: Department of Defense (DOD), General... of appropriate technological collection techniques or other forms of information technology....

  20. Identification and Characterization of the Hemophore-Dependent Heme Acquisition System of Yersinia pestis

    PubMed Central

    Rossi, Maria-Silvia; Fetherston, Jacqueline D.; Létoffé, Sylvie; Carniel, Elisabeth; Perry, Robert D.; Ghigo, Jean-Marc

    2001-01-01

    Yersinia pestis possesses a heme-protein acquisition system (Hmu) that allows it to utilize heme and heme-protein complexes as the sole sources of iron. Analysis of the Y. pestis CO92 genomic sequence revealed a second heme-protein acquisition gene cluster that shares homology with the hemophore-dependent heme acquisition system (Has system) of Serratia marcescens. This locus consisted of the hasRyp receptor gene, the hasAyp hemophore gene, and genes encoding components of the HasAyp dedicated ABC transporter factor (hasDEyp), as well as a tonB homologue (hasByp). By using a reconstituted secretion system in Escherichia coli, we showed that HasAyp is a secreted heme-binding protein and that expression of HasAyp is iron regulated in E. coli. The use of a transcriptional reporter fusion showed that the hasRADEB promoter is Fur regulated and has increased activity at 37°C. Hemoglobin utilization via the Hasyp system was studied with both E. coli and Y. pestis, for which has and has hmu mutant strains were used. No contribution of the Has system to heme utilization was observed in either E. coli or Y. pestis under the conditions we tested. Previously it was shown that a deletion of the Hmu system had no effect on the virulence of Y. pestis in a mouse model of bubonic plague. An Hmu− Has− double mutant also retained full virulence in this model of infection. This report constitutes the first attempt to investigate the contribution of the hemophore-dependent heme acquisition system in bacterial pathogenicity. PMID:11598042

  1. 21. Perimeter acquisition radar building room #200, electrical equipment room ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. Perimeter acquisition radar building room #200, electrical equipment room - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

  2. 75 FR 82576 - Federal Acquisition Regulation; Terminating Contracts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-30

    ...DoD, GSA, and NASA are issuing a final rule amending the Federal Acquisition Regulation (FAR) to clarify the applicability of procedures regarding the termination of contracts to the acquisition of commercial...

  3. 24. Perimeter acquisition radar building room #203, communications room ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. Perimeter acquisition radar building room #203, communications room - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

  4. 22. Perimeter acquisition radar building room #201, phase shifter service ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Perimeter acquisition radar building room #201, phase shifter service platform (level two) - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

  5. 36. Perimeter acquisition radar building, phase shifter service platform; level ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. Perimeter acquisition radar building, phase shifter service platform; level three - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

  6. 78 FR 41331 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 225 Defense Federal Acquisition Regulation Supplement... Supplement (DFARS) to insert a hyperlink and direct contracting officers to the DFARS Procedures,...

  7. 77 FR 19128 - Defense Federal Acquisition Regulation Supplement; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 203 Defense Federal Acquisition Regulation Supplement... Supplement (DFARS) to add a reference for reporting suspected lobbying violations. DATES: Effective...

  8. 77 FR 11775 - Defense Federal Acquisition Regulation Supplement; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 252 Defense Federal Acquisition Regulation Supplement... Supplement (DFARS) to provide needed editorial changes. DATES: Effective Date: February 28, 2012. FOR...

  9. 78 FR 36113 - Defense Federal Acquisition Regulation Supplement; Technical Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 222 Defense Federal Acquisition Regulation Supplement... Supplement (DFARS) to provide needed editorial changes. DATES: Effective Date: June 17, 2013. FOR...

  10. 48 CFR 1825.1101 - Acquisition of supplies.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    .... 1825.1101 Section 1825.1101 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS FOREIGN ACQUISITION Solicitation Provisions and Contract Clauses 1825.1101... accordance with FAR subpart 25.9 and 1825.903....

  11. 48 CFR 1825.1101 - Acquisition of supplies.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... 1825.1101 Section 1825.1101 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS FOREIGN ACQUISITION Solicitation Provisions and Contract Clauses 1825.1101... accordance with FAR subpart 25.9 and 1825.903....

  12. 48 CFR 1825.1101 - Acquisition of supplies.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... 1825.1101 Section 1825.1101 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS FOREIGN ACQUISITION Solicitation Provisions and Contract Clauses 1825.1101... accordance with FAR subpart 25.9 and 1825.903....

  13. 48 CFR 1825.1101 - Acquisition of supplies.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... 1825.1101 Section 1825.1101 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS FOREIGN ACQUISITION Solicitation Provisions and Contract Clauses 1825.1101... accordance with FAR subpart 25.9 and 1825.903....

  14. 48 CFR 1825.1101 - Acquisition of supplies.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    .... 1825.1101 Section 1825.1101 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE ADMINISTRATION SOCIOECONOMIC PROGRAMS FOREIGN ACQUISITION Solicitation Provisions and Contract Clauses 1825.1101... accordance with FAR subpart 25.9 and 1825.903....

  15. 10 CFR 490.305 - Acquisitions satisfying the mandate.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ....305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The... light duty vehicle (regardless of the model year of manufacture), capable of operating on...

  16. 10 CFR 490.305 - Acquisitions satisfying the mandate.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ....305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The... light duty vehicle (regardless of the model year of manufacture), capable of operating on...

  17. 10 CFR 490.305 - Acquisitions satisfying the mandate.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ....305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The... light duty vehicle (regardless of the model year of manufacture), capable of operating on...

  18. 10 CFR 490.305 - Acquisitions satisfying the mandate.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ....305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The... light duty vehicle (regardless of the model year of manufacture), capable of operating on...

  19. 10 CFR 490.305 - Acquisitions satisfying the mandate.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....305 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ALTERNATIVE FUEL TRANSPORTATION PROGRAM Alternative Fuel Provider Vehicle Acquisition Mandate § 490.305 Acquisitions satisfying the mandate. The... light duty vehicle (regardless of the model year of manufacture), capable of operating on...

  20. 28. Perimeter acquisition radar building room #302, signal process and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Perimeter acquisition radar building room #302, signal process and analog receiver room - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND