Science.gov

Sample records for act regulations decisions

  1. Language and Legal Speech Acts: Decisions.

    ERIC Educational Resources Information Center

    Kevelson, Roberta

    The first part of this essay argues specifically that legal speech acts are not statements but question/answer constructions. The focus in this section is on the underlying interrogative structure of the legal decision. The second part of the paper touches on significant topics related to the concept of legal speech acts, including the philosophic…

  2. 77 FR 15368 - Clean Water Act; Availability of List Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-15

    ... AGENCY Clean Water Act; Availability of List Decisions AGENCY: Environmental Protection Agency (EPA... availability of the Environmental Protection Agency's (EPA) proposed decision identifying water quality limited segments and associated pollutants in Oregon to be listed pursuant to section 303(d)(2) of the Clean...

  3. 78 FR 27233 - Clean Water Act: Availability of List Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... AGENCY Clean Water Act: Availability of List Decisions AGENCY: Environmental Protection Agency (EPA... identifying water quality limited segments and associated pollutants in Louisiana to be listed pursuant to Clean Water Act Section 303(d), and request for public comment. Section 303(d) requires that...

  4. 78 FR 20912 - Clean Water Act: Availability of List Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-08

    ... AGENCY Clean Water Act: Availability of List Decisions AGENCY: Environmental Protection Agency (EPA). ACTION: Notice and initial request for public input. SUMMARY: The Clean Water Act requires that States... existing technology-based pollution controls are not stringent enough to attain or maintain State...

  5. 75 FR 37287 - Privacy Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-29

    ... / Tuesday, June 29, 2010 / Rules and Regulations#0;#0; ] RECOVERY ACCOUNTABILITY AND TRANSPARENCY BOARD 4 CFR Part 200 RIN 0430-AA03 Privacy Act Regulations AGENCY: Recovery Accountability and Transparency Board ACTION: Final rule. SUMMARY: The Recovery Accountability and Transparency Board (Board) amends...

  6. 75 FR 41370 - Regulations Implementing the Freedom of Information Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ..., 121 Stat. 2524. The OPEN Government Act amended various FOIA administrative procedures, such as when... procedural rules, final Commission decisions since 1972, final administrative law judges' decisions since 1993, administrative law judges' decisions pending Commission review, strategic plans,...

  7. 77 FR 54909 - Clean Water Act: Availability of List Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... AGENCY Clean Water Act: Availability of List Decisions AGENCY: Environmental Protection Agency (EPA... certain water quality limited waters and the associated pollutant to be listed pursuant to the Clean Water... technology-based pollution controls are not stringent enough to attain or maintain State water...

  8. 78 FR 45925 - Clean Water Act: Availability of List Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ... AGENCY Clean Water Act: Availability of List Decisions AGENCY: Environmental Protection Agency (EPA... waterbodies were added by EPA because the applicable numeric water quality standards marine criterion for... 303(d) List can be obtained at EPA Region 6's Web site at...

  9. 75 FR 82217 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-30

    ... Joint Final Rule The agencies' CRA regulations establish CRA performance standards for small and... between the standards used to evaluate savings associations and the standards used to evaluate banks. 72... Procedure Act (APA), an agency may, for good cause, find (and incorporate the finding and a brief...

  10. 76 FR 79529 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... of the Joint Final Rule The agencies' CRA regulations establish CRA performance standards for small... the Administrative Procedure Act (APA), an agency may, for good cause, find (and incorporate the.... Under 5 U.S.C. 553(d)(3) of the APA, the required publication or service of a substantive rule shall...

  11. 77 FR 75521 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-21

    ... CFR Part 228 FEDERAL DEPOSIT INSURANCE CORPORATION 12 CFR Part 345 RIN 3064-AD90 Community... agencies) are amending their Community Reinvestment Act (CRA) regulations to adjust the asset-size..., 2013. FOR FURTHER INFORMATION CONTACT: OCC: Margaret Hesse, Special Counsel, Community and Consumer...

  12. To Act or Not to Act: Endocannabinoid/Dopamine Interactions in Decision-Making

    PubMed Central

    Hernandez, Giovanni; Cheer, Joseph F.

    2015-01-01

    Decision-making is an ethologically adaptive construct that is impaired in multiple psychiatric disorders. Activity within the mesocorticolimbic dopamine system has been traditionally associated with decision-making. The endocannabinoid system through its actions on inhibitory and excitatory synapses modulates dopamine activity and decision-making. The aim of this brief review is to present a synopsis of available data obtained when the endocannabinoid system is manipulated and dopamine activity recorded. To this end, we review research using different behavioral paradigms to provide further insight into how this ubiquitous signaling system biases dopamine-related behaviors to regulate decision-making. PMID:26733830

  13. Medical decision making and the Human Rights Act 1998.

    PubMed

    Loughrey, J

    2001-01-01

    At present in the UK, when there is conflict of opinion between relatives and health care professionals regarding the treatment of incompetent patients, the courts generally support the latter over the former. This article examines the potential impact of the Human Rights Act 1998, which incorporates the European Convention on Human Rights into UK law, on this position. The possibility of challenges by relatives to disputed decisions on the grounds of Articles 2,3,8 and 14 is examined in the light of relevant Convention jurisprudence. It concludes that the Act will not necessarily result in relatives' views taking priority over doctors', given that the domestic test of the patient's best interests may not infringe the Convention. However, more account will have to be taken of relatives' views given the requirement for the courts to adopt a rights based analysis and to take a more pro-active role in scrutinising medical decision making.

  14. Perceptual decisions are biased by the cost to act

    PubMed Central

    Hagura, Nobuhiro; Haggard, Patrick; Diedrichsen, Jörn

    2017-01-01

    Perceptual decisions are classically thought to depend mainly on the stimulus characteristics, probability and associated reward. However, in many cases, the motor response is considered to be a neutral output channel that only reflects the upstream decision. Contrary to this view, we show that perceptual decisions can be recursively influenced by the physical resistance applied to the response. When participants reported the direction of the visual motion by left or right manual reaching movement with different resistances, their reports were biased towards the direction associated with less effortful option. Repeated exposure to such resistance on hand during perceptual judgements also biased subsequent judgements using voice, indicating that effector-dependent motor costs not only biases the report at the stage of motor response, but also changed how the sensory inputs are transformed into decisions. This demonstrates that the cost to act can influence our decisions beyond the context of the specific action. DOI: http://dx.doi.org/10.7554/eLife.18422.001 PMID:28219479

  15. 78 FR 17176 - Federal Acquisition Regulation; Defense Base Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-20

    ... Federal Acquisition Regulation; Defense Base Act AGENCIES: Department of Defense (DoD), General Services... Act as extended by the Defense Base Act. DATES: Interested parties should submit written comments to... Act as extended by the Defense Base Act. II. Discussion and Analysis The Defense Base Act of...

  16. The Kentucky Civil Rights Act: Explanation, the Act, Regulations.

    ERIC Educational Resources Information Center

    Kentucky State Commission on Human Rights, Frankfort.

    The Kentucky Civil Rights Act, introduced on January 4, 1966, enacted January 27, 1966 and effective July 1, 1966 is said to meet the requirements of the Federal Civil Rights Act of 1964. In 1968, the Act was amended to prohibit housing discrimination. In 1972, the coverage of the Act was extended to prohibit employment discrimination because of…

  17. The French Space Operations Act: Technical Regulations

    NASA Astrophysics Data System (ADS)

    Lazare, B.

    2013-12-01

    The French Space Operations Act (FSOA) [1] stipulates that one of the National Technical Regulations' prime objectives is to protect people, property, public health and the environment. Compliance with these Technical Regulations has been mandatory since 10 December, 2010 for space operations by French space operators and for space operations conducted on French territory. The space safety requirements and regulations governing procedures are based on national and international best practices and experience. A critical design review of the space system and procedures shall be carried out by applicant space operators, in order to verify compliance with the Technical Regulations. An independent technical assessment of the operation is delegated to CNES. The principles applied when drafting the Technical Regulations are as follows: requirements must, as far as possible, establish the rules according to the objective to be obtained, rather than how it is to be achieved; requirements must give preference to international standards recognised as being state of the art; requirements must take previous experience into account. The Technical Regulations are divided into three sections covering requirements common to the launch, control and return of a space object. A special section will cover specific rules to be applied at the Guiana Space Centre. The main topics addressed by the Technical Regulations are: operator safety management system; study of risks to people, property, public health and the Earth's environment; impact study on the outer space environment: space debris generated by the operation; planetary protection. The first version of the Technical Regulations [2], issued in March 2011, is dedicated to unmanned space systems.

  18. 76 FR 43585 - Bank Secrecy Act Regulations; Definitions and Other Regulations Relating to Money Services...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-21

    ... Regulations; Definitions and Other Regulations Relating to Money Services Businesses AGENCY: Treasury... Proposed Rulemaking, Definitions and Other Regulations Relating to Money Services Businesses, 74 FR 22129... to the Bank Secrecy Act Regulations-- Definitions and Other Regulations Relating to Money...

  19. 24 CFR 180.700 - Action upon issuance of a final decision in Fair Housing Act cases.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Action upon issuance of a final decision in Fair Housing Act cases. 180.700 Section 180.700 Housing and Urban Development Regulations Relating to Housing and Urban Development OFFICE OF ASSISTANT SECRETARY FOR EQUAL OPPORTUNITY,...

  20. 24 CFR 180.700 - Action upon issuance of a final decision in Fair Housing Act cases.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Action upon issuance of a final decision in Fair Housing Act cases. 180.700 Section 180.700 Housing and Urban Development Regulations Relating to Housing and Urban Development OFFICE OF ASSISTANT SECRETARY FOR EQUAL OPPORTUNITY,...

  1. 24 CFR 180.700 - Action upon issuance of a final decision in Fair Housing Act cases.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Action upon issuance of a final decision in Fair Housing Act cases. 180.700 Section 180.700 Housing and Urban Development Regulations Relating to Housing and Urban Development OFFICE OF ASSISTANT SECRETARY FOR EQUAL OPPORTUNITY,...

  2. 24 CFR 180.700 - Action upon issuance of a final decision in Fair Housing Act cases.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Action upon issuance of a final decision in Fair Housing Act cases. 180.700 Section 180.700 Housing and Urban Development Regulations Relating to Housing and Urban Development OFFICE OF ASSISTANT SECRETARY FOR EQUAL OPPORTUNITY,...

  3. 24 CFR 180.700 - Action upon issuance of a final decision in Fair Housing Act cases.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Action upon issuance of a final decision in Fair Housing Act cases. 180.700 Section 180.700 Housing and Urban Development Regulations Relating to Housing and Urban Development OFFICE OF ASSISTANT SECRETARY FOR EQUAL OPPORTUNITY,...

  4. 75 FR 61035 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-04

    ... Education loan program; or (2) any private education loan as defined in the Truth in Lending Act, including... expenses,'' each of which is defined in the HEOA in the context of the Truth in Lending Act (TILA). Section... the Truth in Lending Act, as added by section 1011 of the HEOA. In turn, the HEOA defines a...

  5. NASA Principal Center for Review of Clean Air Act Regulations

    NASA Technical Reports Server (NTRS)

    Clark-Ingram, Marceia; Munafo, Paul M. (Technical Monitor)

    2002-01-01

    The Clean Air Act (CAA) regulations have greatly impacted materials and processes utilized in the manufacture of aerospace hardware. Code JE/ NASA's Environmental Management Division at NASA Headquarters recognized the need for a formal, Agency-wide review process of CAA regulations. Marshall Space Flight Center (MSFC) was selected as the 'Principal Center for Review of Clean Air Act Regulations'. This presentation describes the centralized support provided by MSFC for the management and leadership of NASA's CAA regulation review process.

  6. 75 FR 52735 - Clean Water Act Section 303(d): Availability of List Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-27

    ... AGENCY Clean Water Act Section 303(d): Availability of List Decisions AGENCY: Environmental Protection... EPA's decision identifying 12 water quality limited waterbodies and associated pollutants in South Dakota to be listed pursuant to the Clean Water Act Section 303(d)(2), and requests public...

  7. 78 FR 79283 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-30

    ... CRA purposes by the OCC, Board, and FDIC on August 2, 2005, effective September 1, 2005. 70 FR 44256... Act (HMDA). 70 FR 12148 (Mar. 11, 2005). See 12 U.S.C. 2808; 12 CFR 203.2(e)(1). On March 22, 2007... other federal banking agencies in its CRA rule previously set forth at 12 CFR 563e. 72 FR 13429 (Mar....

  8. Regulation of dietary choice by the decision-making circuitry.

    PubMed

    Rangel, Antonio

    2013-12-01

    To advance our understanding of how the brain makes food decisions, it is essential to combine knowledge from two fields that have not yet been well integrated: the neuro-computational basis of decision-making and the homeostatic regulators of feeding. This Review integrates these two literatures from a neuro-computational perspective, with an emphasis in describing the variables computed by different neural systems and how they affect dietary choice. We highlight what is unique about feeding decisions, the mechanisms through which metabolic and endocrine factors affect the decision-making circuitry, why making healthy food choices is difficult for many people, and key processes at work in the obesity epidemic.

  9. 75 FR 36270 - Appraisal Subcommittee; Appraiser Regulation; Privacy Act Implementation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-25

    ... INSTITUTIONS EXAMINATION COUNCIL 12 CFR Part 1102 Appraisal Subcommittee; Appraiser Regulation; Privacy Act Implementation AGENCY: Appraisal Subcommittee of the Federal Financial Institutions Examination Council...-7577 or alice@asc.gov ; Appraisal Subcommittee; 1401 H Street, NW., Suite 760, Washington, DC...

  10. 77 FR 57043 - Regulations Under the Fur Products Labeling Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ...The Federal Trade Commission proposes to amend its Regulations under the Fur Products Labeling Act to update its Fur Products Name Guide, provide more labeling flexibility, incorporate recently enacted Truth in Fur Labeling Act provisions, and eliminate unnecessary requirements. The Commission does not propose changing or providing alternatives to the required name on labels for nyctereutes......

  11. 77 FR 74347 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-14

    ... GENERAL ON INTEGRITY AND EFFICIENCY 5 CFR Chapter XCVIII Freedom of Information Act Regulations AGENCY... implement the Freedom of Information Act (FOIA). The final rule describes the policies and procedures for... provide the procedures and guidelines under which CIGIE will implement the Freedom of Information...

  12. 77 FR 66539 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ... CONCILIATION SERVICE 29 CFR Part 1401 RIN 3076-AA06 Freedom of Information Act Regulations AGENCY: Federal... Mediation and Conciliation Service (FMCS) rules relating to the Freedom of Information Act (FOIA). The final... Administrative practice and procedure, Freedom of information. For the reasons stated in the preamble,...

  13. 76 FR 21871 - Arbitration Panel Decision Under the Randolph-Sheppard Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-19

    ...), under the Act and its implementing regulations in 34 CFR part 395. The Complainant alleged that the SLA violated the Act, the implementing regulations, and State rules and regulations by suspending his vending... complaint from DCH alleging that the Complainant had poor sanitary conditions at Capitol View and...

  14. 77 FR 75362 - Government in the Sunshine Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-20

    ... From the Federal Register Online via the Government Publishing Office EQUAL EMPLOYMENT OPPORTUNITY COMMISSION 29 CFR Part 1612 Government in the Sunshine Act Regulations CFR Correction 0 In Title 29 of the Code of Federal Regulations, Parts 900 to 1899, revised as of July 1, 2012, on page 266, in Sec....

  15. 77 FR 4288 - Arbitration Panel Decision Under the Randolph-Sheppard Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-27

    ...) alleged the United States Department of Veterans Affairs (DVA) violated the Act and its implementing... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION Arbitration Panel Decision Under the Randolph-Sheppard Act AGENCY: Department of Education. ACTION: Notice...

  16. Adaptive Decision Aiding in Computer-Assisted Instruction: Adaptive Computerized Training System (ACTS).

    ERIC Educational Resources Information Center

    Hopf-Weichel, Rosemarie; And Others

    This report describes results of the first year of a three-year program to develop and evaluate a new Adaptive Computerized Training System (ACTS) for electronics maintenance training. (ACTS incorporates an adaptive computer program that learns the student's diagnostic and decision value structure, compares it to that of an expert, and adapts the…

  17. Decision making and effort in the self-regulation of hypertension: testing two competing theories.

    PubMed

    Taylor, Stephanie D; Bagozzi, Richard P; Gaither, Caroline A

    2005-11-01

    We compare the ability of two social psychological models to explain self-regulation decisions to control hypertension by 208 patients at a hospital clinic: the theory of planned behaviour (TPB) and the model of goal-directed behaviour (MGB). The sample was drawn from patients at a large research hospital in North America. The findings show that the MGB not only explains significantly more variance in decision making than the TPB, but it provides an account for how reasons for acting become integrated and transformed into intentions to act, which the TPB does not address. The MGB does this in part by introducing the variable, desire, as an essential mediator between reasons for acting and intentions. The MGB also incorporates the effects of anticipated emotions on decision making, which are forms of forward-looking counterfactual thinking with respect to goals. In addition, the present study reconceptualized instrumental behaviour to encompass how hard one tries to act in the senses of (1) devoting time to planning with respect to reducing/maintaining blood pressure, (2) expending mental/physical energy to reduce/maintain blood pressure, (3) maintaining will power to reduce/maintain blood pressure, and (4) sustaining self-discipline (e.g. in overcoming obstacles) to reduce/maintain blood pressure. Key differences, as well as commonalities, in decision making are pointed-out between men and women and between people whose goal is to reduce versus maintain blood pressure.

  18. Regulation of dietary choice by the decision-making circuitry

    PubMed Central

    Rangel, Antonio

    2014-01-01

    To advance our understanding of how the brain makes food decisions, it is essential to combine knowledge from two fields that have not yet been well integrated: the neuro-computational basis of decision-making and the homeostatic regulators of feeding. This Review integrates these two literatures from a neuro-computational perspective, with an emphasis in describing the variables computed by different neural systems and how they affect dietary choice. We highlight what is unique about feeding decisions, the mechanisms through which metabolic and endocrine factors affect the decision-making circuitry, why making healthy food choices is difficult for many people, and key processes at work in the obesity epidemic. PMID:24270272

  19. Shared Decision Aids: Increasing Patient Acceptance of Long-Acting Reversible Contraception

    PubMed Central

    George, Tracy P.; DeCristofaro, Claire; Dumas, Bonnie P.; Murphy, Pamela F.

    2015-01-01

    Unintended pregnancies are an important public health issue. Long-acting reversible contraceptive methods (LARCs) are reliable, safe, highly effective methods for most women; however they are underutilized in the United States. Shared decision aids were added to usual care in five public health family planning clinics in the Southeastern United States, staffed by advance practice nurses and registered nurses. All five sites showed an increase in the use of LARCs during the time period that shared decision aids were used (results statistically significant to p < 0.001). It is important for women to make informed choices about contraception, and shared decision aids can be utilized to support this decision making. This resource has been adopted for statewide use in all public health clinics, and implications for practice suggest that the use of shared decision aids is an effective method to support informed patient decision making and acceptance of LARC methods of contraception. PMID:27417757

  20. 78 FR 6216 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Office of the Secretary 43 CFR Part 2 RIN 1093-AA15 Freedom of Information Act Regulations AGENCY: Office of the Secretary, Interior. ACTION: Final rule; correction. SUMMARY: This document...

  1. 76 FR 78977 - Real Estate Settlement Procedures Act (Regulation X)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    ... December 20, 2011 Part II Bureau of Consumer Financial Protection 12 CFR Part 1024 Real Estate Settlement... RIN 3170-AA06 Real Estate Settlement Procedures Act (Regulation X) AGENCY: Bureau of Consumer... Department of Housing and Urban Development's (HUD's) rulemaking authority for the Real Estate...

  2. NASA's Principal Center for Review of Clean Air Act Regulations

    NASA Technical Reports Server (NTRS)

    Clark-Ingram, Marceia

    2003-01-01

    Marshall Space Flight Center (MSFC) was selected as the Principal Center for review of Clean Air Act (CAA) regulations. The CAA Principal Center is tasked to: 1) Provide centralized support to NASA/HDQ Code JE for the management and leadership of NASA's CAA regulation review process; 2) Identify potential impact from proposed CAA regulations to NASA program hardware and supporting facilities. The Shuttle Environmental Assurance Initiative, one of the responsibilities of the NASA CAA Working Group (WG), is described in part of this viewgraph presentation.

  3. Balancing act creating the right regulation for coal combustion waste

    SciTech Connect

    Manuel, J.

    2009-11-15

    The December 2008 collapse of a coal ash pond in Tennessee threw safe management of coal combustion waste (CCW) into the spotlight. Millions of tons of CCW are produced in the United States each year, and a large percentage of that is recycled. The US Environmental Protection Agency is pursuing a host of initiatives that could directly or indirectly affect the disposition of CCW. States, too, are taking a look at how they regulate CCW. Among the options is the possibility of regulating CCW under the Resource Conservation and Recovery Act, a move that could have far-reaching implications for both the recycling and the disposal of this waste.

  4. 75 FR 48955 - Arbitration Panel Decision Under the Randolph-Sheppard Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ... Illinois Department of Human Services, Division of Rehabilitation Services v. United States Postal Service... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION Arbitration Panel Decision Under the Randolph-Sheppard Act AGENCY: Department of Education. ACTION: Notice...

  5. 75 FR 48954 - Arbitration Panel Decision Under the Randolph-Sheppard Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ... violations by the United States Department of Defense, Department of the Air Force (Air Force) of the... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION Arbitration Panel Decision Under the Randolph-Sheppard Act AGENCY: Department of Education. ACTION: Notice...

  6. 76 FR 62061 - Clean Water Act Section 303(d): Availability of List Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ... AGENCY Clean Water Act Section 303(d): Availability of List Decisions AGENCY: Environmental Protection... added by EPA because the applicable numeric water quality standards marine criterion for dissolved....epa.gov/region6/water/npdes/tmdl/index.htm#303dlists , or by writing or calling Ms. Diane Smith...

  7. [Aliens Act regulations and consequences of inability to travel].

    PubMed

    Wollenweber, H-M

    2003-12-01

    The question as to whether a foreign national is (un)able to travel or not is often in the foreground of the decision-making process concerning a possible termination of their stay in the Federal Republic of Germany. Authorities responsible for aliens and administrative courts often require accredited medical reports to be able to decide whether inability to travel is actually the case. There are no clear legal criteria with regard to the definition of this term and an explicit provision by law is also missing. In cases where the decision has been made against inability to travel (i.e. a person is said to be able to travel), there may be considerable risks for the person concerned. Where the decision has been made in favour of inability to travel (i.e. the person is said to be unable to travel), a protective system based on the Aliens' Act takes effect. This article gives an overview of the legal consequences arising from a determined inability to travel; the legal difficulties with regard to the definition of this term; and the requirements to be made concerning clarity of a prognosis regarding possible risks. The pertinent legislation as well as opinions expressed in expert literature are presented.

  8. Mechanics Regulates Fate Decisions of Human Embryonic Stem Cells

    PubMed Central

    Sun, Yubing; Villa-Diaz, Luis G.; Lam, Raymond H. W.; Chen, Weiqiang; Krebsbach, Paul H.; Fu, Jianping

    2012-01-01

    Research on human embryonic stem cells (hESCs) has attracted much attention given their great potential for tissue regenerative therapy and fundamental developmental biology studies. Yet, there is still limited understanding of how mechanical signals in the local cellular microenvironment of hESCs regulate their fate decisions. Here, we applied a microfabricated micromechanical platform to investigate the mechanoresponsive behaviors of hESCs. We demonstrated that hESCs are mechanosensitive, and they could increase their cytoskeleton contractility with matrix rigidity. Furthermore, rigid substrates supported maintenance of pluripotency of hESCs. Matrix mechanics-mediated cytoskeleton contractility might be functionally correlated with E-cadherin expressions in cell-cell contacts and thus involved in fate decisions of hESCs. Our results highlighted the important functional link between matrix rigidity, cellular mechanics, and pluripotency of hESCs and provided a novel approach to characterize and understand mechanotransduction and its involvement in hESC function. PMID:22615930

  9. Love as a regulative ideal in surrogate decision making.

    PubMed

    Stonestreet, Erica Lucast

    2014-10-01

    This discussion aims to give a normative theoretical basis for a "best judgment" model of surrogate decision making rooted in a regulative ideal of love. Currently, there are two basic models of surrogate decision making for incompetent patients: the "substituted judgment" model and the "best interests" model. The former draws on the value of autonomy and responds with respect; the latter draws on the value of welfare and responds with beneficence. It can be difficult to determine which of these two models is more appropriate for a given patient, and both approaches may seem inadequate for a surrogate who loves the patient. The proposed "best judgment" model effectively draws on the values incorporated in each of the traditional standards, but does so because these values are important to someone who loves a patient, since love responds to the patient as the specific person she is.

  10. Regulated protein kinases and phosphatases in cell cycle decisions.

    PubMed

    Novak, Bela; Kapuy, Orsolya; Domingo-Sananes, Maria Rosa; Tyson, John J

    2010-12-01

    Many aspects of cell physiology are controlled by protein kinases and phosphatases, which together determine the phosphorylation state of targeted substrates. Some of these target proteins are themselves kinases or phosphatases or other components of a regulatory network characterized by feedback and feed-forward loops. In this review we describe some common regulatory motifs involving kinases, phosphatases, and their substrates, focusing particularly on bistable switches involved in cellular decision processes. These general principles are applied to cell cycle transitions, with special emphasis on the roles of regulated phosphatases in orchestrating progression from one phase to the next of the DNA replication-division cycle.

  11. Safe drinking water act: Amendments, regulations and standards

    SciTech Connect

    Calabrese, E.J.; Gilbert, C.E.; Pastides, H.

    1989-01-01

    This book approaches the topic of safe drinking water by communicating how the EPA has responded to the mandates of Congress. Chapter 1 summarizes what is and will be involved in achieving safe drinking water. Chapter 2 describes the historical development of drinking water regulations. Chapter 3 summarizes the directives of the Safe Drinking Water Act Amendments of 1986. Chapters 4 through 9 discuss each phase of the regulatory program in turn. Specific problems associated with volatile organic chemicals, synthetic organics, inorganic chemicals, and microbiological contaminants are assessed in Chapter 4 and 5. The unique characteristics of radionuclides and their regulation are treated in Chapter 6. The disinfection process and its resultant disinfection by-products are presented in Chapter 7. The contaminant selection process and the additional contaminants to be regulated by 1989 and 1991 and in future years are discussed in Chapters 8 and 9. EPA's Office of Drinking Water's Health Advisory Program is explained in Chapter 10. The record of public water system compliance with the primary drinking water regulations is detailed in Chapter 11. Chapter 12 offers a nongovernmental perspective on the general quality of drinking water and how this is affected by a wide range of drinking water treatment technologies. Separate abstracts are processed for 5 chapters in this book for inclusion in the appropriate data bases.

  12. 76 FR 78483 - S.A.F.E. Mortgage Licensing Act (Regulations G & H)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    ... PROTECTION 12 CFR Part 1007 and 1008 RIN 3170-AA06 S.A.F.E. Mortgage Licensing Act (Regulations G & H) AGENCY... Enforcement for Mortgage Licensing Act (S.A.F.E. Act), the Bureau is publishing for public comment an interim final rule establishing a new Regulation G (S.A.F.E. Mortgage Licensing Act--Federal Registration...

  13. Making decisions about decision-making: conscience, regulation, and the law.

    PubMed

    Miola, José

    2015-01-01

    The exercise of conscience can have far reaching effects. Poor behaviour can be fatal, as it has occurred in various medical scandals over the years. This article takes a wide definition of conscience as its starting point, and argues that the decision-making processes open to society--legal regulation and professional regulation--can serve to limit the options available to an individual and thus her ability to exercise her conscience. The article charts the law's changing attitude to legal intervention, which now seeks to limit the use of conscience by individuals, and addresses concerns that this may serve to 'de-moralise' medicine. It also examines the reasons for this legal change of approach.

  14. 48 CFR 52.222-13 - Compliance with Davis-Bacon and Related Act Regulations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...-Bacon and Related Act Regulations. 52.222-13 Section 52.222-13 Federal Acquisition Regulations System... Text of Provisions and Clauses 52.222-13 Compliance with Davis-Bacon and Related Act Regulations. As prescribed in 22.407(a), insert the following clause: Compliance With Davis-Bacon and Related Act...

  15. 48 CFR 52.222-13 - Compliance with Davis-Bacon and Related Act Regulations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...-Bacon and Related Act Regulations. 52.222-13 Section 52.222-13 Federal Acquisition Regulations System... Text of Provisions and Clauses 52.222-13 Compliance with Davis-Bacon and Related Act Regulations. As prescribed in 22.407(a), insert the following clause: Compliance With Davis-Bacon and Related Act...

  16. 48 CFR 52.222-13 - Compliance with Davis-Bacon and Related Act Regulations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...-Bacon and Related Act Regulations. 52.222-13 Section 52.222-13 Federal Acquisition Regulations System... Text of Provisions and Clauses 52.222-13 Compliance with Davis-Bacon and Related Act Regulations. As prescribed in 22.407(a), insert the following clause: Compliance With Davis-Bacon and Related Act...

  17. 48 CFR 52.222-13 - Compliance with Davis-Bacon and Related Act Regulations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-Bacon and Related Act Regulations. 52.222-13 Section 52.222-13 Federal Acquisition Regulations System... Text of Provisions and Clauses 52.222-13 Compliance with Davis-Bacon and Related Act Regulations. As prescribed in 22.407(a), insert the following clause: Compliance With Davis-Bacon and Related Act...

  18. The Relative Predictive Validity of ACT Scores and High School Grades in Making College Admission Decisions. Issues in College Success

    ERIC Educational Resources Information Center

    ACT, Inc., 2008

    2008-01-01

    Postsecondary institutions often consider students' high school grades and ACT scores when making admission decisions. This issue brief summarizes ACT research on the relative weights of ACT scores and high school grades for predicting college persistence as well as selected indicators of academic success in college. (Contains 1 table and 3…

  19. Regulations Under the Americans With Disabilities Act; Genetic Information Nondiscrimination Act. Final rule.

    PubMed

    2016-05-17

    The Equal Employment Opportunity Commission (EEOC or Commission) is issuing its final rule to amend the regulations and interpretive guidance implementing Title I of the Americans with Disabilities Act (ADA) to provide guidance on the extent to which employers may use incentives to encourage employees to participate in wellness programs that ask them to respond to disability-related inquiries and/or undergo medical examinations. This rule applies to all wellness programs that include disability-related inquiries and/or medical examinations whether they are offered only to employees enrolled in an employer-sponsored group health plan, offered to all employees regardless of whether they are enrolled in such a plan, or offered as a benefit of employment by employers that do not sponsor a group health plan or group health insurance. Published elsewhere in this issue of the Federal Register, the EEOC also issued a final rule to amend the regulations implementing Title II of the Genetic Information Nondiscrimination Act (GINA) that addresses the extent to which employers may offer incentives for an employee's spouse to participate in a wellness program.

  20. 77 FR 43081 - Federal Acquisition Regulation; Information Collection; Buy American Act-Free Trade Agreements...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-23

    ... Regulation; Information Collection; Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate... previously approved information collection requirement concerning the Buy American Act--Free Trade Agreements..., Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate, by any of the...

  1. Adaptive Equilibrium Regulation: A Balancing Act in Two Timescales.

    PubMed

    Boker, Steven M

    2015-03-01

    An equilibrium involves a balancing of forces. Just as one maintains upright posture in standing or walking, many self-regulatory and interpersonal behaviors can be framed as a balancing act between an ever changing environment and within-person processes. The emerging balance between person and environment, the equilibria, are dynamic and adaptive in response to development and learning. A distinction is made between equilibrium achieved solely due to a short timescale balancing of forces and a longer timescale preferred equilibrium which we define as a state towards which the system slowly adapts. Together, these are developed into a framework that this article calls Adaptive Equilibrium Regulation (ÆR), which separates a regulatory process into two timescales: a faster regulation that automatically balances forces and a slower timescale adaptation process that reconfigures the fast regulation so as to move the system towards its preferred equilibrium when an environmental force persists over the longer timescale. This way of thinking leads to novel models for the interplay between multiple timescales of behavior, learning, and development.

  2. Effect of the Family and Medical Leave Act on the operation of cafeteria plans. Final regulations.

    PubMed

    2001-10-17

    This document contains final regulations relating to cafeteria plans that reflect changes made by the Family and Medical Leave Act of 1993 (Act). The final regulations provide the public with guidance needed to comply with the Act and affect employees who participate in cafeteria plans.

  3. 78 FR 72057 - Rules and Regulations Under the Wool Products Labeling Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... CFR Part 300 Rules and Regulations Under the Wool Products Labeling Act AGENCY: Federal Trade... proposed amendments to the Rules and Regulations under the Wool Products Labeling Act of 1939 (``Wool Rules... Commission issued the Wool Rules to implement the Wool Products Labeling Act of 1939, 15 U.S.C. 68-68j....

  4. 77 FR 29982 - Federal Acquisition Regulation; Submission for OMB Review; Davis Bacon Act-Price Adjustment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... Regulation; Submission for OMB Review; Davis Bacon Act-Price Adjustment (Actual Method) AGENCY: Department of... previously approved information collection requirement concerning the Davis-Bacon Act price adjustment... Bacon Act-Price Adjustment (Actual Method), by any of the following methods: Regulations.gov :...

  5. 75 FR 24862 - Removing Regulations Implementing the Fish and Wildlife Conservation Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-06

    ... Wildlife Conservation Act AGENCY: Fish and Wildlife Service, Interior. ACTION: Proposed rule. SUMMARY: We... and Wildlife Conservation Act of 1980. The Act authorized financial and technical assistance to States... through 86. The regulations at part 83 implement the Fish and Wildlife Conservation Act of 1980 (16...

  6. 20 CFR 626.4 - Table of contents for the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Table of contents for the Job Training..., DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.4 Table of contents for the Job Training Partnership Act regulations. The table of contents for the regulations...

  7. 29 CFR 2530.200a-2 - Treasury regulations for purposes of the Act.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Treasury regulations for purposes of the Act. 2530.200a-2... RETIREMENT INCOME SECURITY ACT OF 1974 RULES AND REGULATIONS FOR MINIMUM STANDARDS FOR EMPLOYEE PENSION BENEFIT PLANS Scope and General Provisions § 2530.200a-2 Treasury regulations for purposes of the...

  8. 29 CFR 1903.2 - Posting of notice; availability of the Act, regulations and applicable standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 5 2014-07-01 2014-07-01 false Posting of notice; availability of the Act, regulations and applicable standards. 1903.2 Section 1903.2 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL... § 1903.2 Posting of notice; availability of the Act, regulations and applicable standards. (a)(1)...

  9. 75 FR 58387 - Federal Acquisition Regulation; Information Collection; American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... Regulation; Information Collection; American Recovery and Reinvestment Act-Reporting Requirements--One-Time Reporting, Compensation Requirements AGENCY: Department of Defense (DOD), General Services Administration... Recovery and Reinvestment Act-Reporting Requirements--One Time- Reporting, Compensation...

  10. 75 FR 58389 - Federal Acquisition Regulation; Information Collection; American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... Regulation; Information Collection; American Recovery and Reinvestment Act-Reporting Requirements--One Time Reporting Requirements for Prime Contractors AGENCY: Department of Defense (DOD), General Services... American Recovery and Reinvestment Act-Reporting Requirements--One Time Reporting Requirements for...

  11. 75 FR 58388 - Federal Acquisition Regulation; Information Collection; American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... Regulation; Information Collection; American Recovery and Reinvestment Act-Reporting Requirements--Quarterly Reporting for Prime Contractors AGENCY: Department of Defense (DOD), General Services Administration (GSA... Recovery and Reinvestment Act--Quarterly Reporting for Prime Contractors. Public comments are...

  12. Clinical Decision-Making in the Treatment of Schizophrenia: Focus on Long-Acting Injectable Antipsychotics

    PubMed Central

    Samalin, Ludovic; Garnier, Marion; Auclair, Candy; Llorca, Pierre-Michel

    2016-01-01

    The purpose of this study was to identify clinician characteristics associated with higher prescription rates of long-acting injectable (LAI) antipsychotics, as well as the sources that influence medical decision-making regarding the treatment of schizophrenia. We surveyed 202 psychiatrists during six regional French conferences (Bordeaux, Lyon, Marseille, Nice, Paris, and Strasbourg). Data on the characteristics of practice, prescription rates of antipsychotic, and information sources about their clinical decisions were collected. Most psychiatrists used second-generation antipsychotics (SGAs), and preferentially an oral formulation, in the treatment of schizophrenia. LAI SGAs were prescribed to 30.4% of schizophrenic patients. The duration and type of practice did not influence the class or formulation of antipsychotics used. The clinicians following the higher percentage of schizophrenic patients were associated with a higher use of LAI antipsychotics and a lower use of oral SGAs. Personal experience, government regulatory approval, and guidelines for the treatment of schizophrenia were the three main contributing factors guiding clinicians’ decision-making regarding the treatment of schizophrenia. The more clinicians follow schizophrenic patients, the more they use LAI antipsychotics. The development of specialized programs with top specialists should lead to better use of LAI antipsychotics in the treatment of schizophrenia. PMID:27869767

  13. Clinical Decision-Making in the Treatment of Schizophrenia: Focus on Long-Acting Injectable Antipsychotics.

    PubMed

    Samalin, Ludovic; Garnier, Marion; Auclair, Candy; Llorca, Pierre-Michel

    2016-11-19

    The purpose of this study was to identify clinician characteristics associated with higher prescription rates of long-acting injectable (LAI) antipsychotics, as well as the sources that influence medical decision-making regarding the treatment of schizophrenia. We surveyed 202 psychiatrists during six regional French conferences (Bordeaux, Lyon, Marseille, Nice, Paris, and Strasbourg). Data on the characteristics of practice, prescription rates of antipsychotic, and information sources about their clinical decisions were collected. Most psychiatrists used second-generation antipsychotics (SGAs), and preferentially an oral formulation, in the treatment of schizophrenia. LAI SGAs were prescribed to 30.4% of schizophrenic patients. The duration and type of practice did not influence the class or formulation of antipsychotics used. The clinicians following the higher percentage of schizophrenic patients were associated with a higher use of LAI antipsychotics and a lower use of oral SGAs. Personal experience, government regulatory approval, and guidelines for the treatment of schizophrenia were the three main contributing factors guiding clinicians' decision-making regarding the treatment of schizophrenia. The more clinicians follow schizophrenic patients, the more they use LAI antipsychotics. The development of specialized programs with top specialists should lead to better use of LAI antipsychotics in the treatment of schizophrenia.

  14. Homeostatic regulation of memory systems and adaptive decisions.

    PubMed

    Mizumori, Sheri J Y; Jo, Yong Sang

    2013-11-01

    While it is clear that many brain areas process mnemonic information, understanding how their interactions result in continuously adaptive behaviors has been a challenge. A homeostatic-regulated prediction model of memory is presented that considers the existence of a single memory system that is based on a multilevel coordinated and integrated network (from cells to neural systems) that determines the extent to which events and outcomes occur as predicted. The "multiple memory systems of the brain" have in common output that signals errors in the prediction of events and/or their outcomes, although these signals differ in terms of what the error signal represents (e.g., hippocampus: context prediction errors vs. midbrain/striatum: reward prediction errors). The prefrontal cortex likely plays a pivotal role in the coordination of prediction analysis within and across prediction brain areas. By virtue of its widespread control and influence, and intrinsic working memory mechanisms. Thus, the prefrontal cortex supports the flexible processing needed to generate adaptive behaviors and predict future outcomes. It is proposed that prefrontal cortex continually and automatically produces adaptive responses according to homeostatic regulatory principles: prefrontal cortex may serve as a controller that is intrinsically driven to maintain in prediction areas an experience-dependent firing rate set point that ensures adaptive temporally and spatially resolved neural responses to future prediction errors. This same drive by prefrontal cortex may also restore set point firing rates after deviations (i.e. prediction errors) are detected. In this way, prefrontal cortex contributes to reducing uncertainty in prediction systems. An emergent outcome of this homeostatic view may be the flexible and adaptive control that prefrontal cortex is known to implement (i.e. working memory) in the most challenging of situations. Compromise to any of the prediction circuits should result in

  15. Homeostatic Regulation of Memory Systems and Adaptive Decisions

    PubMed Central

    Mizumori, Sheri JY; Jo, Yong Sang

    2013-01-01

    While it is clear that many brain areas process mnemonic information, understanding how their interactions result in continuously adaptive behaviors has been a challenge. A homeostatic-regulated prediction model of memory is presented that considers the existence of a single memory system that is based on a multilevel coordinated and integrated network (from cells to neural systems) that determines the extent to which events and outcomes occur as predicted. The “multiple memory systems of the brain” have in common output that signals errors in the prediction of events and/or their outcomes, although these signals differ in terms of what the error signal represents (e.g., hippocampus: context prediction errors vs. midbrain/striatum: reward prediction errors). The prefrontal cortex likely plays a pivotal role in the coordination of prediction analysis within and across prediction brain areas. By virtue of its widespread control and influence, and intrinsic working memory mechanisms. Thus, the prefrontal cortex supports the flexible processing needed to generate adaptive behaviors and predict future outcomes. It is proposed that prefrontal cortex continually and automatically produces adaptive responses according to homeostatic regulatory principles: prefrontal cortex may serve as a controller that is intrinsically driven to maintain in prediction areas an experience-dependent firing rate set point that ensures adaptive temporally and spatially resolved neural responses to future prediction errors. This same drive by prefrontal cortex may also restore set point firing rates after deviations (i.e. prediction errors) are detected. In this way, prefrontal cortex contributes to reducing uncertainty in prediction systems. An emergent outcome of this homeostatic view may be the flexible and adaptive control that prefrontal cortex is known to implement (i.e. working memory) in the most challenging of situations. Compromise to any of the prediction circuits should result

  16. 77 FR 19455 - Regulations Implementing the Byrd Amendments to the Black Lung Benefits Act: Determining Coal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ...This document contains proposed regulations implementing amendments to the Black Lung Benefits Act (BLBA or Act) made by the Patient Protection and Affordable Care Act (ACA). The ACA amended the BLBA in two ways. First, it revived a rebuttable presumption of total disability or death due to pneumoconiosis for certain claims. Second, it reinstituted derivative entitlement to benefits for......

  17. 76 FR 24027 - Federal Acquisition Regulation; Information Collection; Buy American Act Certificate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... Regulation; Information Collection; Buy American Act Certificate AGENCY: Department of Defense (DOD), General... previously approved information collection requirement concerning the Buy American Act certificate. Public...-mail cecelia.davis@gsa.gov . SUPPLEMENTARY INFORMATION: A. Purpose The Buy American Act requires...

  18. 75 FR 14547 - Tribal Consultation on Draft Buy Indian Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-26

    ... Bureau of Indian Affairs 48 CFR Chapter 14 RIN 1076-AE95 Tribal Consultation on Draft Buy Indian Act... comments concerning draft regulations to implement the Buy Indian Act. See the SUPPLEMENTARY INFORMATION... implementation of the Buy Indian Act, 25 U.S.C. 47, which provides authority to set aside procurement...

  19. 77 FR 13328 - Federal Acquisition Regulation; Information Collection; Davis Bacon Act-Price Adjustment (Actual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... Regulation; Information Collection; Davis Bacon Act--Price Adjustment (Actual Method) AGENCY: Department of... previously approved information collection requirement concerning the Davis-Bacon Act price adjustment... comments identified by Information Collection 9000- 0154, Davis Bacon Act--Price Adjustment (Actual...

  20. 75 FR 3665 - Regulations under the Comprehensive Smokeless Tobacco Health Education Act; Termination of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-22

    ...The Federal Trade Commission (``FTC'' or ``Commission'') has terminated its rulemaking concerning a proposed amendment to its regulations (``smokeless tobacco regulations''), , implementing the Comprehensive Smokeless Tobacco Health Education Act of 1986 (``Smokeless Tobacco Act''). The proposed amendment expressly provided that sponsored racing vehicles and other event-related objects that......

  1. 20 CFR 626.2 - Format of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Format of the Job Training Partnership Act regulations. 626.2 Section 626.2 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.2 Format of the...

  2. 20 CFR 626.2 - Format of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Format of the Job Training Partnership Act regulations. 626.2 Section 626.2 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.2 Format of the...

  3. 76 FR 82117 - Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-30

    ...This final rule contains regulations implementing amendments to the Longshore and Harbor Workers' Compensation Act (LHWCA) by the American Recovery and Reinvestment Act of 2009 (ARRA), relating to the exclusion of certain recreational-vessel workers from the LHWCA's definition of ``employee.'' These regulations clarify both the definition of ``recreational vessel'' and those circumstances......

  4. 20 CFR 626.2 - Format of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Format of the Job Training Partnership Act regulations. 626.2 Section 626.2 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.2 Format of the...

  5. 78 FR 41835 - Inflation Adjustments to the Price-Anderson Act Financial Protection Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-12

    ... Federal Regulations is sold by the Superintendent of Documents. #0;Prices of new books are listed in the... 3150-AJ25 Inflation Adjustments to the Price-Anderson Act Financial Protection Regulations AGENCY: Nuclear Regulatory Commission. ACTION: Final rule. SUMMARY: The Atomic Energy Act of 1954, as amended...

  6. 77 FR 234 - Rules and Regulations Under the Textile Fiber Products Identification Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-04

    ...; ] FEDERAL TRADE COMMISSION 16 CFR Part 303 Rules and Regulations Under the Textile Fiber Products... Rules and Regulations under the Textile Fiber Products Identification Act (``Textile Rules'').\\1\\ The... the Textile Rules to implement the Textile Fiber Products Identification Act, 15 U.S.C. 70-70k....

  7. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Purpose, scope, and applicability of the Job Training Partnership Act regulations. 626.3 Section 626.3 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and...

  8. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Purpose, scope, and applicability of the Job Training Partnership Act regulations. 626.3 Section 626.3 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and...

  9. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Purpose, scope, and applicability of the Job Training Partnership Act regulations. 626.3 Section 626.3 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and...

  10. 76 FR 78121 - Fair Debt Collection Practices Act (Regulation F)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-16

    ... Financial Protection (Bureau), effective July 21, 2011.\\4\\ See sections 1061 and 1089 of the Dodd-Frank Act... Consumer Financial Protection Act of 2010. Public Law 111-203, section 1089(4); 15 U.S.C.1692l(d). II... Law 111-203, section 1089(1); 15 U.S.C. 1692o. B. Authority To Issue an Interim Final Rule...

  11. 78 FR 32595 - Revision of Freedom of Information Act Regulation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ... practices with respect to FOIA. Finally, the rule would use current cost figures in calculating and charging... enacted by the OPEN Government Act of 2007, reflect developments in the case law, and include current cost... increased from that provided for under current Sec. 15.110(c). Duplication costs for media copies would...

  12. 75 FR 22320 - Regulations Implementing the Freedom of Information Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-28

    ... deletion of materials under the FOIA on the responsive record. Second, as a result of the Chief FOIA...) to reflect the more detailed description of those duties set forth under the OPEN Government Act. 5 U... forth in paragraph (g) to include, where technically feasible, marking the exemption under which...

  13. 75 FR 38684 - Federal Acquisition Regulation; FAR Case 2010-008, Recovery Act Subcontract Reporting Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-02

    ... regulations it believes are necessary. OMB has determined that obtaining publicly reported jobs information at... Regulation; FAR Case 2010-008, Recovery Act Subcontract Reporting Procedures AGENCIES: Department of Defense... Defense Acquisition Regulations Council (Councils) have agreed on an interim rule amending the...

  14. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 1 2012-04-01 2011-04-01 true Federal Reserve Regulation Z and Fair Credit Reporting Act... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and Fair... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L....

  15. 78 FR 8963 - Update of Existing Privacy Act-NASA Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-07

    ... SPACE ADMINISTRATION 14 CFR Part 1212 RIN 2700-AD86 Update of Existing Privacy Act--NASA Regulations... document contains corrections to the final regulations (NASA-2012-0005), which were published in the... Jennings, 202-358-0819. SUPPLEMENTARY INFORMATION: Background NASA's final regulations that published...

  16. Enhancing Decision-Making in STSE Education by Inducing Reflection and Self-Regulated Learning

    NASA Astrophysics Data System (ADS)

    Gresch, Helge; Hasselhorn, Marcus; Bögeholz, Susanne

    2017-02-01

    Thoughtful decision-making to resolve socioscientific issues is central to science, technology, society, and environment (STSE) education. One approach for attaining this goal involves fostering students' decision-making processes. Thus, the present study explores whether the application of decision-making strategies, combined with reflections on the decision-making processes of others, enhances decision-making competence. In addition, this study examines whether this process is supported by elements of self-regulated learning, i.e., self-reflection regarding one's own performance and the setting of goals for subsequent tasks. A computer-based training program which involves the resolution of socioscientific issues related to sustainable development was developed in two versions: with and without elements of self-regulated learning. Its effects on decision-making competence were analyzed using a pre test-post test follow-up control-group design ( N = 242 high school students). Decision-making competence was assessed using an open-ended questionnaire that focused on three facets: consideration of advantages and disadvantages, metadecision aspects, and reflection on the decision-making processes of others. The findings suggest that students in both training groups incorporated aspects of metadecision into their statements more often than students in the control group. Furthermore, both training groups were more successful in reflecting on the decision-making processes of others. The students who received additional training in self-regulated learning showed greater benefits in terms of metadecision aspects and reflection, and these effects remained significant two months later. Overall, our findings demonstrate that the application of decision-making strategies, combined with reflections on the decision-making process and elements of self-regulated learning, is a fruitful approach in STSE education.

  17. 75 FR 41788 - Amendment to the Bank Secrecy Act Regulations-Definitions and Other Regulations Relating to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... Financial Crimes Enforcement Network 31 CFR Part 103 RIN 1506-AB07 Amendment to the Bank Secrecy Act Regulations--Definitions and Other Regulations Relating to Prepaid Access AGENCY: Financial Crimes Enforcement.... Steele, Deputy Director, Financial Crimes Enforcement Network. BILLING CODE 4810-02-P...

  18. The application of genetic information for regulatory standard setting under the clean air act: a decision-analytic approach.

    PubMed

    Cullen, Alison C; Corrales, Mark A; Kramer, C Bradley; Faustman, Elaine M

    2008-08-01

    In 2002, the U.S. Environmental Protection Agency (EPA) released an "Interim Policy on Genomics," stating a commitment to developing guidance on the inclusion of genetic information in regulatory decision making. This statement was followed in 2004 by a document exploring the potential implications. Genetic information can play a key role in understanding and quantifying human susceptibility, an essential step in many of the risk assessments used to shape policy. For example, the federal Clean Air Act (CAA) requires EPA to set National Ambient Air Quality Standards (NAAQS) for criteria pollutants at levels to protect even sensitive populations from adverse health effects with an adequate margin of safety. Asthmatics are generally regarded as a sensitive population, yet substantial research gaps in understanding genetic susceptibility and disease have hindered quantitative risk analysis. This case study assesses the potential role of genomic information regarding susceptible populations in the NAAQS process for fine particulate matter (PM(2.5)) under the CAA. In this initial assessment, we model the contribution of a single polymorphism to asthma risk and mortality risk; however, multiple polymorphisms and interactions (gene-gene and gene-environment) are known to play key roles in the disease process. We show that the impact of new information about susceptibility on estimates of population risk or average risk derived from large epidemiological studies depends on the circumstances. We also suggest that analysis of a single polymorphism, or other risk factor such as health status, may or may not change estimates of individual risk enough to alter a particular regulatory decision, but this depends on specific characteristics of the decision and risk information. We also show how new information about susceptibility in the context of the NAAQS for PM(2.5) could have a large impact on the estimated distribution of individual risk. This would occur if a group were

  19. A nucleostemin family GTPase, NS3, acts in serotonergic neurons to regulate insulin signaling and control body size

    PubMed Central

    Kaplan, Daniel D.; Zimmermann, Gregor; Suyama, Kaye; Meyer, Tobias; Scott, Matthew P.

    2008-01-01

    Growth and body size are regulated by the CNS, integrating the genetic developmental program with assessments of an animal’s current energy state and environmental conditions. CNS decisions are transmitted to all cells of the animal by insulin/insulin-like signals. The molecular biology of the CNS growth control system has remained, for the most part, elusive. Here we identify NS3, a Drosophila nucleostemin family GTPase, as a powerful regulator of body size. ns3 mutants reach <60% of normal size and have fewer and smaller cells, but exhibit normal body proportions. NS3 does not act cell-autonomously, but instead acts at a distance to control growth. Rescue experiments were performed by expressing wild-type ns3 in many different cells of ns3 mutants. Restoring NS3 to only 106 serotonergic neurons rescued global growth defects. These neurons are closely apposed with those of insulin-producing neurons, suggesting possible communication between the two neuronal systems. In the brains of ns3 mutants, excess serotonin and insulin accumulate, while peripheral insulin pathway activation is low. Peripheral insulin pathway activation rescues the growth defects of ns3 mutants. The findings suggest that NS3 acts in serotonergic neurons to regulate insulin signaling and thus exert global growth control. PMID:18628395

  20. 75 FR 63425 - Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... of Workers' Compensation Programs 20 CFR Part 701 RIN 1240-AA02 Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels AGENCY: Office of Workers' Compensation... Workers' Compensation Programs (OWCP) is republishing the Notice of Proposed Rulemaking entitled...

  1. 77 FR 56592 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ... language and structure of the FOIA regulations in order to improve the Department's FOIA performance. More... detailed information about Department procedures for making FOIA requests and descriptions of the types of... possible about the specific records or types of records that you are seeking. This will assist the...

  2. Molecular regulation of the mitosis/meiosis decision in multicellular organisms.

    PubMed

    Kimble, Judith

    2011-08-01

    A major step in the journey from germline stem cell to differentiated gamete is the decision to leave the mitotic cell cycle and begin progression through the meiotic cell cycle. Over the past decade, molecular regulators of the mitosis/meiosis decision have been discovered in most of the major model multicellular organisms. Historically, the mitosis/meiosis decision has been closely linked with controls of germline self-renewal and the sperm/egg decision, especially in nematodes and mice. Molecular explanations of those linkages clarify our understanding of this fundamental germ cell decision, and unifying themes have begun to emerge. Although the complete circuitry of the decision is not known in any organism, the recent advances promise to impact key issues in human reproduction and agriculture.

  3. Brightening up: the effect of the Physician Payment Sunshine Act on existing regulation of pharmaceutical marketing.

    PubMed

    Gorlach, Igor; Pham-Kanter, Genevieve

    2013-01-01

    With the passage of the Physician Payment Sunshine Act as part of the federal health care reform law, pharmaceutical manufacturers are now required to disclose a wide range of payments made by manufacturers to physicians. We review current state regulation of pharmaceutical marketing and consider how the federal sunshine provision will affect existing marketing regulation. We analyze the legal and practical implications of the Physician Payment Sunshine Act.

  4. 78 FR 54650 - Federal Acquisition Regulation; Information Collection American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... Regulation; Information Collection American Recovery and Reinvestment Act-Reporting Requirements--One-Time... requirement concerning the American Recovery and Reinvestment Act-Reporting Requirements--One Time Reporting... Requirements-- One Time Reporting for First-tier Subcontractors, by any of the following methods:...

  5. 78 FR 54648 - Federal Acquisition Regulation; Information Collection; American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... Regulation; Information Collection; American Recovery and Reinvestment Act--Reporting Requirements--One-Time... requirement concerning the American Recovery and Reinvestment Act--Reporting Requirements--One Time-Reporting... Requirements-- One Time-Reporting, Compensation Requirements, by any of the following methods:...

  6. 78 FR 57808 - Rules and Regulations Under the Wool Products Labeling Act of 1939

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-20

    ...Based on comments received in response to its Advance Notice of Proposed Rulemaking, the Federal Trade Commission (the ``Commission'' or ``FTC'') proposes amending its rules and regulations under the Wool Products Labeling Act of 1939 (``Wool Rules'' or ``Rules'') to: conform to the requirements of the Wool Suit Fabric Labeling Fairness and International Standards Conforming Act, which revised......

  7. 75 FR 58390 - Federal Acquisition Regulation; Information Collection; American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... Regulation; Information Collection; American Recovery and Reinvestment Act-Reporting Requirements--One-Time Reporting for First-Tier Subcontractors AGENCY: Department of Defense (DOD), General Services Administration... Reinvestment Act-Reporting Requirements--One-Time Reporting for First-Tier Subcontractors. Public comments...

  8. 76 FR 16977 - Regulations To Implement the Equal Employment Provisions of the Americans With Disabilities Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ...The Equal Employment Opportunity Commission (the Commission or the EEOC) issues its final revised Americans with Disabilities Act (ADA) regulations and accompanying interpretive guidance in order to implement the ADA Amendments Act of 2008. The Commission is responsible for enforcement of title I of the ADA, as amended, which prohibits employment discrimination on the basis of disability.......

  9. Identifying Specific Learning Disabilities: Legislation, Regulation, and Court Decisions

    ERIC Educational Resources Information Center

    Zumeta, Rebecca O.; Zirkel, Perry A.; Danielson, Louis

    2014-01-01

    Specific learning disability (SLD) identification and eligibility practices are evolving and sometimes contentious. This article describes the historical context and current status of the SLD definition, legislation, regulation, and case law related to the identification of students eligible for special education services. The first part traces…

  10. What do we know about the application of the Mental Capacity Act (2005) in healthcare practice regarding decision-making for frail and older people? A systematic literature review.

    PubMed

    Hinsliff-Smith, Kathryn; Feakes, Ruth; Whitworth, Gillian; Seymour, Jane; Moghaddam, Nima; Dening, Tom; Cox, Karen

    2017-03-01

    In England and Wales, decision-making in cases of uncertain mental capacity is regulated by the Mental Capacity Act 2005. The Act provides a legal framework for decision-making for adults (16 and over) who are shown to lack capacity and where best interest decisions need to be made on their behalf. Frail older people with cognitive impairments represent a growing demographic sector across England and Wales for whom the protective principles of the Act have great relevance, as they become increasingly dependent on the care of others. However, while the Act articulates core principles, applying the Act in everyday healthcare contexts raises challenges for care providers in terms of interpretation and application. This paper presents a review of the published evidence documenting the use of the Act in healthcare practice, with particular reference to frail older people. Our aim was to identify, review and critically evaluate published empirical studies concerned with the implementation and application of the Act in healthcare settings. A systematic approach was undertaken with pre-determined exclusion and inclusion criteria applied across five electronic bibliographic databases combined with a manual search of specific journals. This review reports on 38 empirical sources which met the inclusion criteria published between 2005 and 2013. From the 38 sources, three descriptive themes were identified: knowledge and understanding, implementation and tensions in applying the Act, and alternative perspectives of the Act. There is a need for improved knowledge and conceptualisation to enable successful incorporation of the Act into everyday care provision. Inconsistencies in the application of the Act are apparent across a variety of care settings. This review suggest staff need more opportunities to engage, learn and implement the Act, in order for it to have greater resonance to their individual practice and ultimately benefit patient care.

  11. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  12. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  13. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  14. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  15. 77 FR 38717 - Updating Regulations Issued Under the Fair Labor Standards Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-29

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Wage and Hour Division 29 CFR Parts 531 and 553 Updating Regulations Issued Under the Fair Labor Standards Act CFR Correction In Title 29 of the Code of Federal Regulations, Parts 500 to 899, revised as of July...

  16. Regulating Emotions during Difficult Multiattribute Decision Making: The Role of Pre-Decisional Coherence Shifting

    PubMed Central

    Carpenter, Stephanie M.; Yates, J. Frank; Preston, Stephanie D.; Chen, Lydia

    2016-01-01

    Almost all real-life decisions entail attribute conflict; every serious choice alternative is better than its competitors on some attribute dimensions but worse on others. In pre-decisional “coherence shifting,” the decision maker gradually softens that conflict psychologically to the point where one alternative is seen as dominant over its competitors, or nearly so. Specifically, weaknesses of the eventually chosen alternative come to be perceived as less severe and less important while its strengths seem more desirable and significant. The research described here demonstrates that difficult multiattribute decision problems are aversive and that pre-decisional coherence shifting aids individuals in regulating that emotional discomfort. Across three studies, attribute conflict was confirmed to be aversive (Study 1), and skin conductance responses and ratings of decision difficulty both decreased in participants who coherence shifted (Study 2). Coherence shifting was also diminished among decision makers who were depleted of regulatory resources, known to be required for common emotion regulation mechanisms. Further, coherence shifting was shown to be relatively common among people who reported strong suppression tendencies in everyday emotion regulation (Study 3). Overall, the data suggest that, at least in part, coherence shifting serves as a tool that helps decision makers manage the pre-decisional discomfort generated by attribute conflict. Theoretical and practical implications are discussed. PMID:26986752

  17. Regulating Emotions during Difficult Multiattribute Decision Making: The Role of Pre-Decisional Coherence Shifting.

    PubMed

    Carpenter, Stephanie M; Yates, J Frank; Preston, Stephanie D; Chen, Lydia

    2016-01-01

    Almost all real-life decisions entail attribute conflict; every serious choice alternative is better than its competitors on some attribute dimensions but worse on others. In pre-decisional "coherence shifting," the decision maker gradually softens that conflict psychologically to the point where one alternative is seen as dominant over its competitors, or nearly so. Specifically, weaknesses of the eventually chosen alternative come to be perceived as less severe and less important while its strengths seem more desirable and significant. The research described here demonstrates that difficult multiattribute decision problems are aversive and that pre-decisional coherence shifting aids individuals in regulating that emotional discomfort. Across three studies, attribute conflict was confirmed to be aversive (Study 1), and skin conductance responses and ratings of decision difficulty both decreased in participants who coherence shifted (Study 2). Coherence shifting was also diminished among decision makers who were depleted of regulatory resources, known to be required for common emotion regulation mechanisms. Further, coherence shifting was shown to be relatively common among people who reported strong suppression tendencies in everyday emotion regulation (Study 3). Overall, the data suggest that, at least in part, coherence shifting serves as a tool that helps decision makers manage the pre-decisional discomfort generated by attribute conflict. Theoretical and practical implications are discussed.

  18. A Primer on Decision Analysis for Individually Prescribed Instruction. ACT Technical Bulletin No. 17.

    ERIC Educational Resources Information Center

    Davis, Charles E.; And Others

    A coherent system of decision making is described that may be incorporated into an instructional sequence to provide a supplement to the experience-based judgment of the classroom teacher. The elements of this decision process incorporate prior information such as a teacher's past experience, experimental results such as a test score, and…

  19. Technology diffusion and environmental regulation: Evidence from electric power plants under the Clean Air Act

    NASA Astrophysics Data System (ADS)

    Frey, Elaine F.

    Even though environmental policy can greatly affect the path of technology diffusion, the economics literature contains limited empirical evidence of this relationship. My research will contribute to the available evidence by providing insight into the technology adoption decisions of electric generating firms. Since policies are often evaluated based on the incentives they provide to promote adoption of new technologies, it is important that policy makers understand the relationship between technological diffusion and regulation structure to make informed decisions. Lessons learned from this study can be used to guide future policies such as those directed to mitigate climate change. I first explore the diffusion of scrubbers, a sulfur dioxide (SO 2) abatement technology, in response to federal market-based regulations and state command-and-control regulations. I develop a simple theoretical model to describe the adoption decisions of scrubbers and use a survival model to empirically test the theoretical model. I find that power plants with strict command-and-control regulations have a high probability of installing a scrubber. These findings suggest that although market-based regulations have encouraged diffusion, many scrubbers have been installed because of state regulatory pressure. Although tradable permit systems are thought to give firms more flexibility in choosing abatement technologies, I show that interactions between a permit system and pre-existing command-and-control regulations can limit that flexibility. In a separate analysis, I explore the diffusion of combined cycle (CC) generating units, which are natural gas-fired generating units that are cleaner and more efficient than alternative generating units. I model the decision to consider adoption of a CC generating unit and the extent to which the technology is adopted in response to environmental regulations imposed on new sources of pollutants. To accomplish this, I use a zero-inflated Poisson

  20. Delegation of Authority Under the Community Environmental Response Facilitation Act (CERFA) - Decision Memorandum

    EPA Pesticide Factsheets

    This memorandum concerns how the Office of Enforcement (OE) proposed that two new authorities under the Community Environmental Response Facilitation Act (CERFA) be delegated to the Regional Administrators.

  1. 76 FR 24291 - Proposed National Marketing Agreement Regulating Leafy Green Vegetables; Recommended Decision and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... April 29, 2011 Part V Department of Agriculture Agricultural Marketing Service 7 CFR Part 970 Proposed National Marketing Agreement Regulating Leafy Green Vegetables; Recommended Decision and Opportunity To File Written Exceptions to Proposed Marketing Agreement No. 970; Proposed Rule #0;#0;Federal...

  2. Neural mechanisms regulating different forms of risk-related decision-making: Insights from animal models.

    PubMed

    Orsini, Caitlin A; Moorman, David E; Young, Jared W; Setlow, Barry; Floresco, Stan B

    2015-11-01

    Over the past 20 years there has been a growing interest in the neural underpinnings of cost/benefit decision-making. Recent studies with animal models have made considerable advances in our understanding of how different prefrontal, striatal, limbic and monoaminergic circuits interact to promote efficient risk/reward decision-making, and how dysfunction in these circuits underlies aberrant decision-making observed in numerous psychiatric disorders. This review will highlight recent findings from studies exploring these questions using a variety of behavioral assays, as well as molecular, pharmacological, neurophysiological, and translational approaches. We begin with a discussion of how neural systems related to decision subcomponents may interact to generate more complex decisions involving risk and uncertainty. This is followed by an overview of interactions between prefrontal-amygdala-dopamine and habenular circuits in regulating choice between certain and uncertain rewards and how different modes of dopamine transmission may contribute to these processes. These data will be compared with results from other studies investigating the contribution of some of these systems to guiding decision-making related to rewards vs. punishment. Lastly, we provide a brief summary of impairments in risk-related decision-making associated with psychiatric disorders, highlighting recent translational studies in laboratory animals.

  3. 78 FR 18344 - Federal Acquisition Regulation; Submission for OMB Review; Buy American Act-Free Trade Agreements...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-26

    ... Regulation; Submission for OMB Review; Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate... previously approved information collection requirement concerning the Buy American Act--Free Trade Agreements... comments identified by Information Collection 9000- 0130, Buy American Act--Free Trade...

  4. Employer Self-Insurance Decisions and the Implications of the Patient Protection and Affordable Care Act as Modified by the Health Care and Education Reconciliation Act of 2010 (ACA).

    PubMed

    Eibner, Christine; Girosi, Federico; Miller, Amalia; Cordova, Amado; McGlynn, Elizabeth A; Pace, Nicholas M; Price, Carter C; Vardavas, Raffaele; Gresenz, Carole Roan

    2011-01-01

    The Patient Protection and Affordable Care Act as amended by the Health Care and Education Reconciliation Act of 2010 (ACA) changes the regulatory environment within which health insurance policies on the small-group market are bought and sold. New regulations include rate bands that limit premium price variation, risk-adjustment policies that will transfer funds from low-actuarial-risk to high-actuarial-risk plans, and requirements that plans include "essential health benefits." While the new regulations will be applied to all non-grandfathered fully insured policies purchased by businesses with 100 or fewer workers, self-insured plans are exempt from these regulations. As a result, some firms may have a stronger incentive to offer self-insured plans after the ACA takes full effect. In this article we identify factors that influence employers' decisions to self-insure and estimate how the ACA will influence self-insurance rates. We also consider the implications of higher self-insurance rates for adverse selection in the non-self-insured small-group market and whether enrollees in self-insured plans receive different benefits than enrollees in fully-insured plans. Results are based on data analysis, literature review, findings from discussions with stakeholders, and microsimulation analysis using the COMPARE model. Overall, we find little evidence that self-insured plans differ systematically from fully insured plans in terms of benefit generosity, price, or claims denial rates. Stakeholders expressed significant concern about adverse selection in the health insurance exchanges due to regulatory exemptions for self-insured plans. However, our microsimulation analysis predicts a sizable increase in self-insurance only if comprehensive stop-loss policies become widely available after the ACA takes full effect and the expected cost of self-insuring with stop-loss is comparable to the cost of being fully insured in a market without rating regulations.

  5. The Decision Analysis Interview Approach in the Collaborative Management of a Large Regulated Water Course

    NASA Astrophysics Data System (ADS)

    Marttunen, Mika; Hämäläinen, Raimo P.

    2008-12-01

    There are always conflicting goals in the management of large water courses. However, by involving stakeholders actively in the planning and decision-making processes, it is possible to work together toward commonly acceptable solutions. In this article, we describe how we applied interactive multicriteria decision analysis (MCDA) in a collaborative process which aimed at an ecologically, socially, and economically sustainable water course regulation policy. The stakeholders’ opinions about the alternative regulation schemes and the relative importance of their impacts were elicited with the HIPRE software. Altogether, 20 personal interactive decision analysis interviews (DAIs) were carried out with the stakeholders. Our experience suggests that the DAIs can considerably improve the quality and efficiency of the collaborative planning process. By improving communication and understanding of the decision situation in the steering group, the approach helped to develop a consensus solution in a case having strong conflicts of interest. In order to gain the full benefits of the MCDA approach, interactive preference elicitation is vital. It is also essential to integrate the approach tightly into the planning and decision-making process. The project’s home pages are available to the public at http://www.paijanne.hut.fi/ .

  6. The decision analysis interview approach in the collaborative management of a large regulated water course.

    PubMed

    Marttunen, Mika; Hämäläinen, Raimo P

    2008-12-01

    There are always conflicting goals in the management of large water courses. However, by involving stakeholders actively in the planning and decision-making processes, it is possible to work together toward commonly acceptable solutions. In this article, we describe how we applied interactive multicriteria decision analysis (MCDA) in a collaborative process which aimed at an ecologically, socially, and economically sustainable water course regulation policy. The stakeholders' opinions about the alternative regulation schemes and the relative importance of their impacts were elicited with the HIPRE software. Altogether, 20 personal interactive decision analysis interviews (DAIs) were carried out with the stakeholders. Our experience suggests that the DAIs can considerably improve the quality and efficiency of the collaborative planning process. By improving communication and understanding of the decision situation in the steering group, the approach helped to develop a consensus solution in a case having strong conflicts of interest. In order to gain the full benefits of the MCDA approach, interactive preference elicitation is vital. It is also essential to integrate the approach tightly into the planning and decision-making process. The project's home pages are available to the public at http://www.paijanne.hut.fi/.

  7. 77 FR 13311 - Arbitration Panel Decision Under the Randolph-Sheppard Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... arbitration panel rendered a decision in the matter of the Rutherford Beard v. Michigan Commission for the... under 20 U.S.C. 107d-1(a), after receiving a complaint from the complainant, Rutherford Beard. Under... facilities on Federal and other property. Background Rutherford Beard (Complainant) alleged that the...

  8. 75 FR 68783 - Clean Water Act Section 303(d): Availability of List Decisions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-09

    .... SUMMARY: This action announces the availability of EPA decisions identifying water quality limited... stringent enough to attain or maintain State water quality standards and for which total maximum daily loads... several water quality limited segments as impaired and additional associated pollutants for several...

  9. 75 FR 71431 - Clean Water Act Section 303(d): Availability of List Decisions Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-23

    ... decisions identifying water quality limited segments and associated pollutants in California to be listed... are not stringent enough to attain or maintain state water quality standards and for which total... not to list several water quality limited segments as impaired and additional associated...

  10. Adaptive Decision Aiding in Computer-Assisted Instruction: Adaptive Computerized Training System (ACTS)

    DTIC Science & Technology

    1980-09-01

    CATALOG NUMBERTechnical Report 475%; , ADAPTIVE DECISION &IDING IN OMPUTER-ASSISTED *JECHNICAL REPOT INSTRUCTION: ADAPTIVE COMPUTERIZED TRAINING )16...alternative. For example, in auto maintenance, the mechanic is trained to adjust the dis- tributor with a "feeler" guage or a dwell tachometer . He

  11. 77 FR 1062 - Arbitration Panel Decision Under the Randolph-Sheppard Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-09

    ...The Department of Education (Department) gives notice that on August 29, 2011, an arbitration panel rendered a decision in the matter of Judy A. Davis-Perry v. Missouri Department of Social Services Rehabilitation Services for the Blind, Case no. R-S/10-1. The Department convened this panel after receiving a complaint filed by the Complainant, Judy A....

  12. 76 FR 75799 - General Administrative Regulations; Mutual Consent Cancellation; Food Security Act of 1985...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-05

    ... Consent Cancellation; Food Security Act of 1985, Implementation; Denial of Benefits; and Ineligibility for... remove Subpart C--General Administrative Regulations; Mutual Consent Cancellation and Subpart F--Food... quality of the human environment, health, or safety. Therefore, neither an Environmental Assessment nor...

  13. 78 FR 70515 - Petition To Promulgate Standards for Bears Under the Animal Welfare Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-26

    ... Promulgate Standards for Bears Under the Animal Welfare Act Regulations AGENCY: Animal and Plant Health... transportation of all species of bears held in captivity except polar bears, for which there are already... pigs and hamsters, rabbits, nonhuman primates, and marine mammals (including polar bears), and...

  14. 77 FR 60620 - Update of Existing Privacy Act-NASA Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-04

    ... From the Federal Register Online via the Government Publishing Office NATIONAL AERONAUTICS AND SPACE ADMINISTRATION 14 CFR Part 1212 RIN 2700-AD86 Update of Existing Privacy Act--NASA Regulations AGENCY: National Aeronautics and Space Administration. ACTION: Direct final rule. SUMMARY: This...

  15. 76 FR 10234 - Amendment to the Bank Secrecy Act Regulations-Reports of Foreign Financial Accounts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ...FinCEN is issuing this final rule to amend the Bank Secrecy Act (BSA) regulations regarding reports of foreign financial accounts. The rule addresses the scope of the persons that are required to file reports of foreign financial accounts. The rule further specifies the types of accounts that are reportable, and provides filing relief in the form of exemptions for certain persons with......

  16. 20 CFR 626.4 - Table of contents for the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ....601Applied VST budgeting. Subpart G—Experimental, Research, and Demonstration Projects 638.710Experimental, research, and demonstration projects. Subpart H—Administrative Provisions 638.800Program management. 638..., DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.4 Table...

  17. Impact of Clean Air Act Regulations on Nitrogen Fate and Transport in Neuse River Basin

    EPA Science Inventory

    This study investigated impacts of Clean Air Act Amendment (CAAA) NOx emissions regulations on the fate and transport of nitrogen for two watersheds in the Neuse River Basin, North Carolina, USA from 1990 to 2020. The Soil and Water Assessment Tool (SWAT) and the Community Multi-...

  18. The National Labor Relations Act and the Regulation of Public Employee Collective Bargaining

    ERIC Educational Resources Information Center

    McCann, Walter; Smiley, Stafford

    1976-01-01

    The arguments for and against federal assumption of the responsibility for regulating the relationship between public employers and public employees are analyzed. It is suggested that the National Labor Relations Act should be extended to include them, thereby imposing upon them a duty to bargain collectively. Available from: the Harvard…

  19. 76 FR 35963 - Disclosure of Information; Privacy Act Regulations; Notice and Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ...) Disclosure to federal financial institutions supervisory agencies and certain other agencies. The Director of... CORPORATION 12 CFR Parts 309 and 310 RIN 3064-AD83 Disclosure of Information; Privacy Act Regulations; Notice... disclosure of information and the PA, as amended. In taking this action the FDIC's goal is to avoid...

  20. 78 FR 32547 - Loan Originator Compensation Requirements Under the Truth in Lending Act (Regulation Z...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ... PROTECTION 12 CFR Part 1026 RIN 3170-AA37 Loan Originator Compensation Requirements Under the Truth in... Compensation Requirements under the Truth in Lending Act (Regulation Z) Final Rule, issued on January 20, 2013...\\ One of these final rules was the Loan Originator Compensation Requirements Under the Truth in...

  1. 76 FR 20569 - Horse Protection Act; Petition for Amendments to Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... Society of the United States, the American Society for the Prevention of Cruelty to Animals, the American... Animal and Plant Health Inspection Service 9 CFR Part 11 Horse Protection Act; Petition for Amendments to Regulations AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Notice. SUMMARY: We...

  2. 77 FR 4498 - Rules and Regulations Under the Wool Products Labeling Act of 1939

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-30

    ... meaning set forth in Sec. 303.12 of the Textile Rules. 16 CFR 300.1(k). \\6\\ Federal Trade Commission... ``trimmings'' in Sec. 300.1(k), which incorporates by reference Section 303.12 of the Textile Rules? If so....12 of the Rules and Regulations Under the Textile Fiber Products Identification Act (``Textile...

  3. 75 FR 40754 - Government in the Sunshine Act Regulations of the National Science Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-14

    ... From the Federal Register Online via the Government Publishing Office NATIONAL SCIENCE FOUNDATION 45 CFR Part 614 RIN 3145-AA53 Government in the Sunshine Act Regulations of the National Science Board AGENCY: National Science Board (NSB), National Science Foundation (NSF). ACTION: Direct final...

  4. Rationing health protection: a proposal to exempt nuisance dust from US Clean Air Act regulations.

    PubMed

    Centner, Terence J; Colson, Gregory

    2013-03-15

    The US House of Representative has passed a bill called the "Farm Dust Regulation Prevention Act" (Dust Act) that would exempt most types of particulate matter (PM) in rural areas from the air quality controls of the US Clean Air Act. The Dust Act would markedly change the country's air quality standards. An examination of the proposed provisions shows that they would exempt non-combustion PM pollutants from mining, smelting, petroleum production, and power generation from existing air quality standards. Persons downwind from pollutants generated in rural areas could be exposed to concentrations of carcinogenic heavy metals, asbestos, and benzene known to adversely affect their health and ecological resources. Existing federal air quality standards based on science would be replaced by a flexible standard that rations health protection.

  5. Should the model for risk-informed regulation be game theory rather than decision theory?

    PubMed

    Bier, Vicki M; Lin, Shi-Woei

    2013-02-01

    Risk analysts frequently view the regulation of risks as being largely a matter of decision theory. According to this view, risk analysis methods provide information on the likelihood and severity of various possible outcomes; this information should then be assessed using a decision-theoretic approach (such as cost/benefit analysis) to determine whether the risks are acceptable, and whether additional regulation is warranted. However, this view ignores the fact that in many industries (particularly industries that are technologically sophisticated and employ specialized risk and safety experts), risk analyses may be done by regulated firms, not by the regulator. Moreover, those firms may have more knowledge about the levels of safety at their own facilities than the regulator does. This creates a situation in which the regulated firm has both the opportunity-and often also the motive-to provide inaccurate (in particular, favorably biased) risk information to the regulator, and hence the regulator has reason to doubt the accuracy of the risk information provided by regulated parties. Researchers have argued that decision theory is capable of dealing with many such strategic interactions as well as game theory can. This is especially true in two-player, two-stage games in which the follower has a unique best strategy in response to the leader's strategy, as appears to be the case in the situation analyzed in this article. However, even in such cases, we agree with Cox that game-theoretic methods and concepts can still be useful. In particular, the tools of mechanism design, and especially the revelation principle, can simplify the analysis of such games because the revelation principle provides rigorous assurance that it is sufficient to analyze only games in which licensees truthfully report their risk levels, making the problem more manageable. Without that, it would generally be necessary to consider much more complicated forms of strategic behavior (including

  6. Policy implications of genetic information on regulation under the Clean Air Act: the case of particulate matter and asthmatics.

    PubMed

    Kramer, C Bradley; Cullen, Alison C; Faustman, Elaine M

    2006-03-01

    The U.S. Clean Air Act (CAA) explicitly guarantees the protection of sensitive human subpopulations from adverse health effects associated with air pollution exposure. Identified subpopulations, such as asthmatics, may carry multiple genetic susceptibilities to disease onset and progression and thus qualify for special protection under the CAA. Scientific advances accelerated as a result of the groundbreaking Human Genome Project enable the quantification of genetic information that underlies such human variability in susceptibility and the cellular mechanisms of disease. In epidemiology and regulatory toxicology, genetic information can more clearly elucidate human susceptibility essential to risk assessment, such as in support of air quality regulation. In an effort to encourage the incorporation of genomic information in regulation, the U.S. Environmental Protection Agency (EPA) has issued an Interim Policy on Genomics. Additional research strategy and policy documents from the National Academy of Science, the U.S. EPA, and the U.S. Department of Health and Human Services further promote the expansion of asthma genetics research for human health risk assessment. Through a review of these government documents, we find opportunities for the inclusion of genetic information in the regulation of air pollutants. In addition, we identify sources of information in recent scientific research on asthma genetics relevant to regulatory standard setting. We conclude with recommendations on how to integrate these approaches for the improvement of regulatory health science and the prerequisites for inclusion of genetic information in decision making.

  7. 76 FR 2890 - Arbitration Panel Decision Under the Randolph-Sheppard Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-18

    ...The Department of Education (Department) gives notice that on March 31, 2010, an arbitration panel rendered a decision in the matter of Daniel Czubak v. Illinois Department of Human Services, Division of Rehabilitation Services, Case no. R-S/08-5. This panel was convened by the Department under 20 U.S.C. 107d-1(a), after the Department received a complaint filed by the petitioner, Daniel......

  8. 46 CFR 1.03-35 - Appeals from decisions or actions of a recognized classification society acting on behalf of the...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... classification society acting on behalf of the Coast Guard. 1.03-35 Section 1.03-35 Shipping COAST GUARD... recognized classification society acting on behalf of the Coast Guard. (a) Any person directly affected by a decision or action of a recognized classification society performing plan review, tonnage measurement,...

  9. 46 CFR 1.03-35 - Appeals from decisions or actions of a recognized classification society acting on behalf of the...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... classification society acting on behalf of the Coast Guard. 1.03-35 Section 1.03-35 Shipping COAST GUARD... recognized classification society acting on behalf of the Coast Guard. (a) Any person directly affected by a decision or action of a recognized classification society performing plan review, tonnage measurement,...

  10. 46 CFR 1.03-35 - Appeals from decisions or actions of a recognized classification society acting on behalf of the...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... classification society acting on behalf of the Coast Guard. 1.03-35 Section 1.03-35 Shipping COAST GUARD... recognized classification society acting on behalf of the Coast Guard. (a) Any person directly affected by a decision or action of a recognized classification society performing plan review, tonnage measurement,...

  11. 46 CFR 1.03-35 - Appeals from decisions or actions of a recognized classification society acting on behalf of the...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... classification society acting on behalf of the Coast Guard. 1.03-35 Section 1.03-35 Shipping COAST GUARD... recognized classification society acting on behalf of the Coast Guard. (a) Any person directly affected by a decision or action of a recognized classification society performing plan review, tonnage measurement,...

  12. 46 CFR 1.03-35 - Appeals from decisions or actions of a recognized classification society acting on behalf of the...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... classification society acting on behalf of the Coast Guard. 1.03-35 Section 1.03-35 Shipping COAST GUARD... recognized classification society acting on behalf of the Coast Guard. (a) Any person directly affected by a decision or action of a recognized classification society performing plan review, tonnage measurement,...

  13. 76 FR 6604 - Arbitration Panel Decision Under the Randolph-Sheppard Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    ... Service (FRS), toll-free, at 1-800-877-8339. Individuals with disabilities may obtain this document in an... patently unfair or an abuse of discretion and thus was not in violation of state rules and regulations...

  14. Industry invites regulation: the passage of the Pure Food and Drug Act of 1906.

    PubMed Central

    Barkan, I D

    1985-01-01

    Ending its 27-year stranglehold on proposals for federal pure food and drug legislation, Congress passed the Pure Food and Drug Act and its companion bill, the Meat Inspection Act, on June 30, 1906. An unprecedented convergence of consumer, scientific, and industrial support in 1906 prompted such action; most industries even planned for it, hoping regulation would restore the competitiveness of their products on weak foreign and domestic markets. The ways in which these interests converged, and the reasons therefore, suggest a change in their relationships to each other and with the federal government as America headed into the twentieth century. Images p21-a p21-b PMID:3881052

  15. Genetic technologies and the regulation of reproductive decision-making in Australia.

    PubMed

    Karpin, Isabel; Bennett, Belinda

    2006-08-01

    This article provides a critical analysis of the current Australian regulatory landscape at the interface between genetics and reproductive decision-making. The authors argue that a comparative analysis with other countries and international law and a contextual examination of the way law regulates concepts such as disease and health, abnormality and normality is necessary before we can develop appropriate policy and legislative responses in this area. Specific genetic testing technologies are considered including prenatal genetic testing, preimplantation genetic diagnosis and inheritable genetic modification. An increasing number of members of the Australian community are using genetic testing technologies when they decide to have a baby. The authors argue that as concepts of disease and health vary among members of the community and the potential to test for traits other than illness increases, a new tension arises between an ethic of individual choice and a role for government in regulating reproductive decision-making.

  16. Hematopoietic stem cell fate decisions are regulated by Wnt antagonists: comparisons and current controversies.

    PubMed

    Cain, Corey J; Manilay, Jennifer O

    2013-01-01

    Wingless and int (Wnt) proteins are secreted proteins that are important for regulating hematopoietic stem cell self-renewal and differentiation in the bone marrow microenvironment in mice. The mechanisms by which Wnt signaling regulates these hematopoietic cell fate decisions are not fully understood. Secreted Wnt antagonists, which are expressed in bone and bone marrow stromal cells, either bind to Wnt ligands directly or block Wnt receptors and co-receptors to halt Wnt-mediated signal transduction in both osteolineage and hematopoietic cell types. Secreted frizzled related proteins-1 and -2, Wnt inhibitory factor-1, Dickkopf-1, and Sclerostin are Wnt antagonists that influence hematopoietic cell fate decisions in the bone marrow niche. In this review, we compare and contrast the roles of these Wnt antagonists and their effects on hematopoietic development in mice, and also discuss the clinical significance of targeting Wnt antagonists within the context of hematopoietic disease.

  17. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Are my HCT/P's regulated solely under section 361..., AND CELLULAR AND TISSUE-BASED PRODUCTS General Provisions § 1271.10 Are my HCT/P's regulated solely.../P is regulated solely under section 361 of the PHS Act and the regulations in this part if it...

  18. Modeling Employer Self-Insurance Decisions after the Affordable Care Act

    PubMed Central

    Cordova, Amado; Eibner, Christine; Vardavas, Raffaele; Broyles, James; Girosi, Federico

    2013-01-01

    Objective To present a microsimulation model that addresses the methodological challenge of estimating the firm decision to self-insure. Methodology The model considers the risk that the firm bears when self-insuring and the opportunity to mitigate that risk by purchasing stop-loss insurance. The model makes use of a structural, utility maximization framework to account for numerous aspects of the firm decision, and a multinomial probit to reproduce the elasticity of the firm's demand for health insurance. Findings and Conclusions Our simulations provide three important conclusions. First, they project significant increases in self-insurance rates among small firms--presumably induced by the desire to avoid ACA's rate-banding and risk adjustment regulations—only if generous stop-loss policies become widely available. Second, they show that this increase would be due to this hypothetical adoption of widespread, generous reinsurance by the market and not by passage of the ACA. Third, even with a substantial increase of self-insurance rates among small firms, they project negligible adverse selection in the exchanges, as indicated by our finding that the increase in exchange premium is less than 0.5% when assuming very generous stop-loss policies after implementation of the ACA. PMID:23346976

  19. Jagged2 acts as a Delta-like Notch ligand during early hematopoietic cell fate decisions

    PubMed Central

    Van de Walle, Inge; De Smet, Greet; Gärtner, Martina; De Smedt, Magda; Waegemans, Els; Vandekerckhove, Bart; Leclercq, Georges; Plum, Jean; Aster, Jon C.; Bernstein, Irwin D.; Guidos, Cynthia J.; Kyewski, Bruno

    2011-01-01

    Notch signaling critically mediates various hematopoietic lineage decisions and is induced in mammals by Notch ligands that are classified into 2 families, Delta-like (Delta-like-1, -3 and -4) and Jagged (Jagged1 and Jagged2), based on structural homology with both Drosophila ligands Delta and Serrate, respectively. Because the functional differences between mammalian Notch ligands were still unclear, we have investigated their influence on early human hematopoiesis and show that Jagged2 affects hematopoietic lineage decisions very similarly as Delta-like-1 and -4, but very different from Jagged1. OP9 coculture experiments revealed that Jagged2, like Delta-like ligands, induces T-lineage differentiation and inhibits B-cell and myeloid development. However, dose-dependent Notch activation studies, gene expression analysis, and promoter activation assays indicated that Jagged2 is a weaker Notch1-activator compared with the Delta-like ligands, revealing a Notch1 specific signal strength hierarchy for mammalian Notch ligands. Strikingly, Lunatic-Fringe– mediated glycosylation of Notch1 potentiated Notch signaling through Delta-like ligands and also Jagged2, in contrast to Jagged1. Thus, our results reveal a unique role for Jagged1 in preventing the induction of T-lineage differentiation in hematopoietic stem cells and show an unexpected functional similarity between Jagged2 and the Delta-like ligands. PMID:21372153

  20. 76 FR 14275 - Regulations Issued Under the Export Grape and Plum Act; Revision to the Minimum Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-16

    ... Service 7 CFR Part 35 Regulations Issued Under the Export Grape and Plum Act; Revision to the Minimum... requirements under the Export Grape and Plum Act. This rule changes the minimum bunch weight requirement for... under authority of the Export Grape and Plum Act, as amended (7 U.S.C. 591-599), hereinafter referred...

  1. 77 FR 33635 - Amendment to the Bank Secrecy Act Regulations-Requirement That Clerks of Court Report Certain...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... Financial Crimes Enforcement Network 31 CFR Part 1010 RIN 1506-AB17 Amendment to the Bank Secrecy Act Regulations--Requirement That Clerks of Court Report Certain Currency Transactions AGENCY: Financial Crimes... Financial Transactions Reporting Act of 1970, as amended by the USA PATRIOT Act of 2001 and...

  2. 17 CFR 270.14a-1 - Use of notification pursuant to regulation E under the Securities Act of 1933.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of 1933 by a small business investment company operating under the Small Business Investment Act of... to regulation E under the Securities Act of 1933. 270.14a-1 Section 270.14a-1 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT...

  3. 78 FR 45842 - Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedure Act (Regulation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ... From the Federal Register Online via the Government Publishing Office BUREAU OF CONSUMER FINANCIAL PROTECTION 12 CFR Parts 1024 and 1026 RIN 3170-AA37 Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedure Act (Regulation X) and the Truth in Lending Act (Regulation Z) Correction...

  4. 26 CFR 1.706-2T - Temporary regulations; question and answer under the Tax Reform Act of 1984.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Temporary regulations; question and answer under the Tax Reform Act of 1984. 1.706-2T Section 1.706-2T Internal Revenue INTERNAL REVENUE SERVICE...-2T Temporary regulations; question and answer under the Tax Reform Act of 1984. Question 1:...

  5. Allosteric ACTion: the varied ACT domains regulating enzymes of amino-acid metabolism.

    PubMed

    Lang, Eric J M; Cross, Penelope J; Mittelstädt, Gerd; Jameson, Geoffrey B; Parker, Emily J

    2014-12-01

    Allosteric regulation of enzyme activity plays important metabolic roles. Here we review the allostery of enzymes of amino-acid metabolism conferred by a discrete domain known as the ACT domain. This domain of 60-70 residues has a βαββαβ topology leading to a four-stranded β4β1β3β2 antiparallel sheet with two antiparallel helices on one face. Extensive sequence variation requires a combined sequence/structure/function analysis for identification of the ACT domain. Common features include highly varied modes of self-association of ACT domains, ligand binding at domain interfaces, and transmittal of allosteric signals through conformational changes and/or the manipulation of quaternary equilibria. A recent example illustrates the relatively facile adoption of this versatile module of allostery by gene fusion.

  6. New paradigms for conserved, multifactorial, cis-acting regulation of meiotic recombination

    PubMed Central

    Wahls, Wayne P.; Davidson, Mari K.

    2012-01-01

    How do cells position the Spo11 (Rec12)-dependent initiation of meiotic recombination at hotspots? The mechanisms are poorly understood and a prevailing view is that they differ substantially between phylogenetic groups. However, recent work discovered that individual species have multiple different DNA sequence-specific, protein–DNA complexes that regulate (and are essential for the activation of) recombination hotspots. The cis-acting elements function combinatorially with documented examples of synergism, antagonism and redundancy. Furthermore, we provide evidence that all currently well-defined modules of this multifactorial, cis-acting regulation are conserved functionally between taxa whose latest common ancestor occurred more than 1 billion years ago. Functionally conserved components include the ATF/CREB-family heterodimer Atf1-Pcr1 and its CRE-like DNA site M26, the CCAAT-box-binding complex Php2-Php3-Php5 and the CCAAT-box, and the zinc-finger protein Rst2 and its Oligo-C motif. The newfound multiplicity, functional redundancy and conservation of cis-acting controls constitute a paradigm shift with broad implications. They provide compelling evidence that most meiotic recombination is, like transcription, regulated by sequence-specific protein–DNA complexes. And the new findings provide important mechanistic insight, such as a solution to the conundrum that Prdm9 is a ‘master regulator’ of—yet is dispensable for—hotspot activity in mammals. PMID:22904082

  7. The decision to exclude agricultural and domestic workers from the 1935 Social Security Act.

    PubMed

    DeWitt, Larry

    2010-01-01

    The Social Security Act of 1935 excluded from coverage about half the workers in the American economy. Among the excluded groups were agricultural and domestic workers-a large percentage of whom were African Americans. This has led some scholars to conclude that policymakers in 1935 deliberately excluded African Americans from the Social Security system because of prevailing racial biases during that period. This article examines both the logic of this thesis and the available empirical evidence on the origins of the coverage exclusions. The author concludes that the racial-bias thesis is both conceptually flawed and unsupported by the existing empirical evidence. The exclusion of agricultural and domestic workers from the early program was due to considerations of administrative feasibility involving tax-collection procedures. The author finds no evidence of any other policy motive involving racial bias.

  8. Regulations implementing the Federal Coal Mine Health and Safety Act of 1969, as amended. Employment Standards Administration, Labor. Final rule.

    PubMed

    2000-12-20

    On January 22, 1997, the Department issued a proposed rule to amend the regulations implementing the Black Lung Benefits Act. 62 FR 3338-3435 (Jan. 22, 1997). When the comment period closed on August 21, 1997, the Department had received written submissions from almost 200 interested persons, including coal miners, coal mine operators, insurers, physicians, and attorneys. The Department also held hearings in Charleston, West Virginia, and Washington, D.C. at which over 50 people testified. The Department carefully reviewed the testimony and the comments and, on October 8, 1999, issued a second notice of proposed rulemaking. 64 FR 54966-55072 (Oct. 8, 1999). In its second notice, the Department proposed changing several of the most important provisions in its initial proposal. The Department also explained its decision not to alter the original proposal with respect to other key regulations based on the comments received to date. Finally, the Department prepared an initial regulatory flexibility analysis. In order to ensure that small businesses that could be affected by the Department's proposal received appropriate notice of the Department's proposed changes, the Department mailed a copy of the second notice of proposed rulemaking to all coal mine operators contained in the databases maintained by the Mine Safety and Health Administration. The Department initially allowed interested parties until December 7, 1999 to file comments to its second proposal, but extended that period until January 6, 2000. The Department received 37 written submissions before the close of the comment period, from groups representing both coal miners and coal mine operators. The Department also received comments from individual miners, various coal mining and insurance companies, as well as from claims processing organizations, attorneys, and various professional organizations. The Department has carefully reviewed all of the comments, and is issuing its final rule. The rule contains a

  9. Legal briefing: Shared decision making and patient decision aids.

    PubMed

    Pope, Thaddeus Mason; Hexum, Melinda

    2013-01-01

    This "Legal Briefing" column covers recent legal developments involving patient decision aids.This topic has been the subject of recent articles in JCE. It is included in the 2010 Patient Protection and Affordable Care Act. And it has received significant attention in the biomedical literature, including a new book, a thematic issue of Health Affairs, and a recent article in the New England Journal of Medicine. Moreover, physicians and health systems across the United States are increasingly integrating decision aids into their clinical practice. Both federal and state laws play a significant role in promoting this expanded use. On the other hand, concerns about liability could stymie development and implementation. We categorize legal developments concerning patient decision aids into the following five sections: 1. Development of decision aids. 2. Effectiveness of decision aids. 3. Federal regulation of decision aids. 4. State regulation of decision aids. 5. Legal concerns regarding decision aids.

  10. Numb is not a critical regulator of Notch-mediated cell fate decisions in the developing chick inner ear

    PubMed Central

    Eddison, Mark; Weber, Sara J.; Ariza-McNaughton, Linda; Lewis, Julian; Daudet, Nicolas

    2015-01-01

    The Notch signaling pathway controls differentiation of hair cells and supporting cells in the vertebrate inner ear. Here, we have investigated whether Numb, a known regulator of Notch activity in Drosophila, is involved in this process in the embryonic chick. The chicken homolog of Numb is expressed throughout the otocyst at early stages of development and is concentrated at the basal pole of the cells. It is asymmetrically allocated at some cell divisions, as in Drosophila, suggesting that it could act as a determinant inherited by one of the two daughter cells and favoring adoption of a hair-cell fate. To test the implication of Numb in hair cell fate decisions and the regulation of Notch signaling, we used different methods to overexpress Numb at different stages of inner ear development. We found that sustained or late Numb overexpression does not promote hair cell differentiation, and Numb does not prevent the reception of Notch signaling. Surprisingly, none of the Numb-overexpressing cells differentiated into hair cells, suggesting that high levels of Numb protein could interfere with intracellular processes essential for hair cell survival. However, when Numb was overexpressed early and more transiently during ear development, no effect on hair cell formation was seen. These results suggest that in the inner ear at least, Numb does not significantly repress Notch activity and that its asymmetric distribution in dividing precursor cells does not govern the choice between hair cell and supporting cell fates. PMID:25814931

  11. Application of best practice approaches for designing decision support tools: The preparatory education about clinical trials (PRE-ACT) study

    PubMed Central

    Fleisher, Linda; Ruggieri, Dominique G.; Miller, Suzanne M.; Manne, Sharon; Albrecht, Terrance; Buzaglo, Joanne; Collins, Michael A.; Katz, Michael; Kinzy, Tyler G.; Liu, Tasnuva; Manning, Cheri; Charap, Ellen Specker; Millard, Jennifer; Miller, Dawn M.; Poole, David; Raivitch, Stephanie; Roach, Nancy; Ross, Eric A.; Meropol, Neal J.

    2014-01-01

    Objective This article describes the rigorous development process and initial feedback of the PRE-ACT (Preparatory Education About Clinical Trials) web-based- intervention designed to improve preparation for decision making in cancer clinical trials. Methods The multi-step process included stakeholder input, formative research, user testing and feedback. Diverse teams (researchers, advocates and developers) participated including content refinement, identification of actors, and development of video scripts. Patient feedback was provided in the final production period and through a vanguard group (N = 100) from the randomized trial. Results Patients/advocates confirmed barriers to cancer clinical trial participation, including lack of awareness and knowledge, fear of side effects, logistical concerns, and mistrust. Patients indicated they liked the tool’s user-friendly nature, the organized and comprehensive presentation of the subject matter, and the clarity of the videos. Conclusion The development process serves as an example of operationalizing best practice approaches and highlights the value of a multi-disciplinary team to develop a theory-based, sophisticated tool that patients found useful in their decision making process. Practice implications Best practice approaches can be addressed and are important to ensure evidence-based tools that are of value to patients and supports the usefulness of a process map in the development of e-health tools. PMID:24813474

  12. Slit/Robo signaling regulates cell fate decisions in the intestinal stem cell lineage of Drosophila.

    PubMed

    Biteau, Benoît; Jasper, Heinrich

    2014-06-26

    In order to maintain tissue homeostasis, cell fate decisions within stem cell lineages have to respond to the needs of the tissue. This coordination of lineage choices with regenerative demand remains poorly characterized. Here, we identify a signal from enteroendocrine cells (EEs) that controls lineage specification in the Drosophila intestine. We find that EEs secrete Slit, a ligand for the Robo2 receptor in intestinal stem cells (ISCs) that limits ISC commitment to the endocrine lineage, establishing negative feedback control of EE regeneration. Furthermore, we show that this lineage decision is made within ISCs and requires induction of the transcription factor Prospero in ISCs. Our work identifies a function for the conserved Slit/Robo pathway in the regulation of adult stem cells, establishing negative feedback control of ISC lineage specification as a critical strategy to preserve tissue homeostasis. Our results further amend the current understanding of cell fate commitment within the Drosophila ISC lineage.

  13. 45 CFR 681.35 - Can a party request reconsideration of the initial decision?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... decision? 681.35 Section 681.35 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.35 Can a party... the initial decision with the ALJ within 20 days of receipt of the initial decision. If the...

  14. 45 CFR 681.35 - Can a party request reconsideration of the initial decision?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... decision? 681.35 Section 681.35 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.35 Can a party... the initial decision with the ALJ within 20 days of receipt of the initial decision. If the...

  15. 45 CFR 681.35 - Can a party request reconsideration of the initial decision?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... decision? 681.35 Section 681.35 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.35 Can a party... the initial decision with the ALJ within 20 days of receipt of the initial decision. If the...

  16. The nexus between decision making and emotion regulation: a review of convergent neurocognitive substrates.

    PubMed

    Mitchell, Derek G V

    2011-02-02

    Emotional information, such as reward or punishment, gains rapid and often preferential access to neurocognitive resources. This ability to quickly evaluate and integrate emotion-related information is thought to benefit a range of behaviours critical for survival. Conversely, the improper use of, or preoccupation with, emotional information is associated with disruptions in functioning and psychiatric disorders. Optimally, an organism utilizes emotional information when it is significant, and minimizes its influence when it is not. Recently, similar regions of prefrontal cortex have been identified that are associated with regulating both behavioural conflict (motor response selection or inhibition) and affective conflict (emotional representation and awareness). In this review, data will be examined that concerns this convergence between decision making (modulating what we do) and emotion regulation (modulating how we feel) and an informal model will be proposed linking these processes at a neurocognitive level. The studies reviewed collectively support the conclusion that overlapping areas of prefrontal cortex perform similar computations whether the functional objective is to modulate an operant response, or an emotional one. Specifically, the idea is raised that key aspects of decision making and emotion regulation are bound by a common functional objective in which internal representations of conditioned stimuli and reinforcers are modulated to facilitate optimal behaviour or states. Emphasis is placed on dorsomedial, dorsolateral, ventrolateral, and ventromedial regions of prefrontal cortex.

  17. 78 FR 13298 - Notice of Retrospective Review of the Americans With Disabilities Act Regulations for Over-the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-27

    ... Act Regulations for Over-the-Road Bus Operators; Request for Comments AGENCY: Office of the Secretary...) implementing regulations for over-the-road bus (OTRB) operators. The DOT will review regulations specified in... Stat. 327, 42 U.S.C. 225 and 611), which required the accessibility of new over-the-road buses...

  18. 78 FR 44685 - Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedures Act (Regulation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-24

    ... Final Rule), 78 FR 6407 (Jan. 30, 2013). \\2\\ Mortgage Servicing Rules Under the Real Estate Settlement... Final Rules), 78 FR 10695 (Feb. 14, 2013) (Regulation X), 78 FR 10901 (Feb. 14, 2013) (Regulation Z). \\3\\ Amendments to the 2013 Escrows Final Rule under the Truth in Lending Act (Regulation Z), 78 FR 30739 (May...

  19. Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration

    PubMed Central

    Bernis, Cyril; Swift-Taylor, Beth; Nord, Matthew; Carmona, Sarah; Chook, Yuh Min; Forbes, Douglass J.

    2014-01-01

    The nuclear import receptors importin β and transportin play a different role in mitosis: both act phenotypically as spatial regulators to ensure that mitotic spindle, nuclear membrane, and nuclear pore assembly occur exclusively around chromatin. Importin β is known to act by repressing assembly factors in regions distant from chromatin, whereas RanGTP produced on chromatin frees factors from importin β for localized assembly. The mechanism of transportin regulation was unknown. Diametrically opposed models for transportin action are as follows: 1) indirect action by RanGTP sequestration, thus down-regulating release of assembly factors from importin β, and 2) direct action by transportin binding and inhibiting assembly factors. Experiments in Xenopus assembly extracts with M9M, a superaffinity nuclear localization sequence that displaces cargoes bound by transportin, or TLB, a mutant transportin that can bind cargo and RanGTP simultaneously, support direct inhibition. Consistently, simple addition of M9M to mitotic cytosol induces microtubule aster assembly. ELYS and the nucleoporin 107–160 complex, components of mitotic kinetochores and nuclear pores, are blocked from binding to kinetochores in vitro by transportin, a block reversible by M9M. In vivo, 30% of M9M-transfected cells have spindle/cytokinesis defects. We conclude that the cell contains importin β and transportin “global positioning system”or “GPS” pathways that are mechanistically parallel. PMID:24478460

  20. Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration.

    PubMed

    Bernis, Cyril; Swift-Taylor, Beth; Nord, Matthew; Carmona, Sarah; Chook, Yuh Min; Forbes, Douglass J

    2014-04-01

    The nuclear import receptors importin β and transportin play a different role in mitosis: both act phenotypically as spatial regulators to ensure that mitotic spindle, nuclear membrane, and nuclear pore assembly occur exclusively around chromatin. Importin β is known to act by repressing assembly factors in regions distant from chromatin, whereas RanGTP produced on chromatin frees factors from importin β for localized assembly. The mechanism of transportin regulation was unknown. Diametrically opposed models for transportin action are as follows: 1) indirect action by RanGTP sequestration, thus down-regulating release of assembly factors from importin β, and 2) direct action by transportin binding and inhibiting assembly factors. Experiments in Xenopus assembly extracts with M9M, a superaffinity nuclear localization sequence that displaces cargoes bound by transportin, or TLB, a mutant transportin that can bind cargo and RanGTP simultaneously, support direct inhibition. Consistently, simple addition of M9M to mitotic cytosol induces microtubule aster assembly. ELYS and the nucleoporin 107-160 complex, components of mitotic kinetochores and nuclear pores, are blocked from binding to kinetochores in vitro by transportin, a block reversible by M9M. In vivo, 30% of M9M-transfected cells have spindle/cytokinesis defects. We conclude that the cell contains importin β and transportin "global positioning system"or "GPS" pathways that are mechanistically parallel.

  1. ATH1 and KNAT2 proteins act together in regulation of plant inflorescence architecture.

    PubMed

    Li, Yang; Pi, Limin; Huang, Hai; Xu, Lin

    2012-02-01

    The inflorescence of flowering plants is a highly organized structure, not only contributing to plant reproductive processes, but also constituting an important part of the entire plant morphology. Previous studies have revealed that the class-I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS (BP or KNAT1), KNAT2, and KNAT6 play essential roles in inflorescence architecture. Pedicel morphology is known to contribute greatly to inflorescence architecture, and BP negatively regulates KNAT2 and KNAT6 to ensure that pedicels have a normal upward-pointing orientation. These findings indicate that a genetic network exists in controlling pedicel orientation, but how this network functions in the developmental process remains elusive. Here it is reported that the ARABIDOPSIS THALIANA HOMEOBOX GENE1 (ATH1) gene, which belongs to the BELL1-like homeodomain gene family, is a new member participating in regulating pedicel orientation in the class-I KNOX network. In a genetic screening for suppressors of isoginchaku-2D, a gain-of-function ASYMMETRIC LEAVES2 mutant that displays downward-pointing pedicels, a suppressor mutant was obtained. Characterization of this mutant revealed that the mutation corresponds to ATH1. Genetic analysis indicated that ATH1 acts mainly in the KNAT2 pathway. Yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that ATH1 physically interacts with KNAT2. The data indicate that the ATH1-KNAT2 complex acts redundantly with KNAT6, both of which are negatively regulated by BP during pedicel development.

  2. Identification of trans-acting factors regulating SamDC expression in Oryza sativa

    SciTech Connect

    Basu, Supratim; Roychoudhury, Aryadeep; Sengupta, Dibyendu N.

    2014-03-07

    Highlights: • Identification of cis elements responsible for SamDC expression by in silico analysis. • qPCR analysis of SamDC expression to abiotic and biotic stress treatments. • Detection of SamDC regulators using identified cis-elements as probe by EMSA. • Southwestern Blot analysis to predict the size of the trans-acting factors. - Abstract: Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silico analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the trans-acting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression.

  3. Greenhouse Gas Court Decision

    EPA Pesticide Factsheets

    View the June 26, 2012, U.S. Court of Appeals- D.C. Circuit's decision to uphold EPA's Endangerment Finding and greenhouse gas regulations issued under the Clean Air Act (CAA) for passenger vehicles and CAA permitting for stationary sources.

  4. The European "clinical trial" regulation; relationship with the Jardé Act: a Giens workshop.

    PubMed

    Lemaire, François; Marchenay, Brigitte; Chassany, Olivier; Barthélémy, Philippe; Bouzzagou, Mohamed; Comet, Denis; Delval, Cécile; Dubray, Claude; Fouret, Cécile; Frija-Orvoen, Elisabeth; Gambotti, Laetitia; Lamarque, Véronique; d'Orsay, Geneviève; Plattner, Valérie; Sibenaler, Claire; Roux, Jacques; Thoby, Frédérique

    2015-01-01

    In May 2014, the European Union Parliament and Council published a new regulation on clinical trials on medicinal products for human use, which is designed to replace Directive 2001/20/EC. It will not come into effect until 2016. Nevertheless, it is essential to examine its relationship with national legislation, i.e. the Jardé Act, whose implementation has been delayed pending publication of the European regulation. The Giens workshop identified and examined the various issues that this relationship is bound to raise. In particular, it looked at trial methodology assessment procedures, the working relationship between the French National Agency of Drug Safety and Health Products (Agence Nationale de Sécurité du Médicament et des Produits de Santé, ANSM) and ethics committees during the authorization application evaluation phase, review of post-authorization/registration studies on medicinal products and medical devices, and data transparency.

  5. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly.

    PubMed

    Lettice, Laura A; Horikoshi, Taizo; Heaney, Simon J H; van Baren, Marijke J; van der Linde, Herma C; Breedveld, Guido J; Joosse, Marijke; Akarsu, Nurten; Oostra, Ben A; Endo, Naoto; Shibata, Minoru; Suzuki, Mikio; Takahashi, Eiichi; Shinka, Toshikatsu; Nakahori, Yutaka; Ayusawa, Dai; Nakabayashi, Kazuhiko; Scherer, Stephen W; Heutink, Peter; Hill, Robert E; Noji, Sumihare

    2002-05-28

    Preaxial polydactyly (PPD) is a common limb malformation in human. A number of polydactylous mouse mutants indicate that misexpression of Shh is a common requirement for generating extra digits. Here we identify a translocation breakpoint in a PPD patient and a transgenic insertion site in the polydactylous mouse mutant sasquatch (Ssq). The genetic lesions in both lie within the same respective intron of the LMBR1/Lmbr1 gene, which resides approximately 1 Mb away from Shh. Genetic analysis of Ssq reveals that the Lmbr1 gene is incidental to the phenotype and that the mutation directly interrupts a cis-acting regulator of Shh. This regulator is most likely the target for generating PPD mutations in human.

  6. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly

    PubMed Central

    Lettice, Laura A.; Horikoshi, Taizo; Heaney, Simon J. H.; van Baren, Marijke J.; van der Linde, Herma C.; Breedveld, Guido J.; Joosse, Marijke; Akarsu, Nurten; Oostra, Ben A.; Endo, Naoto; Shibata, Minoru; Suzuki, Mikio; Takahashi, Eiichi; Shinka, Toshikatsu; Nakahori, Yutaka; Ayusawa, Dai; Nakabayashi, Kazuhiko; Scherer, Stephen W.; Heutink, Peter; Hill, Robert E.; Noji, Sumihare

    2002-01-01

    Preaxial polydactyly (PPD) is a common limb malformation in human. A number of polydactylous mouse mutants indicate that misexpression of Shh is a common requirement for generating extra digits. Here we identify a translocation breakpoint in a PPD patient and a transgenic insertion site in the polydactylous mouse mutant sasquatch (Ssq). The genetic lesions in both lie within the same respective intron of the LMBR1/Lmbr1 gene, which resides ≈1 Mb away from Shh. Genetic analysis of Ssq reveals that the Lmbr1 gene is incidental to the phenotype and that the mutation directly interrupts a cis-acting regulator of Shh. This regulator is most likely the target for generating PPD mutations in human. PMID:12032320

  7. PDCD10/CCM3 acts downstream of {gamma}-protocadherins to regulate neuronal survival.

    PubMed

    Lin, Chengyi; Meng, Shuxia; Zhu, Tina; Wang, Xiaozhong

    2010-12-31

    γ-Protocadherins (PCDH-γ) regulate neuronal survival in the vertebrate central nervous system. The molecular mechanisms of how PCDH-γ mediates this function are still not understood. In this study, we show that through their common cytoplasmic domain, different PCDH-γ isoforms interact with an intracellular adaptor protein named PDCD10 (programmed cell death 10). PDCD10 is also known as CCM3, a causative genetic defect for cerebral cavernous malformations in humans. Using RNAi-mediated knockdown, we demonstrate that PDCD10 is required for the occurrence of apoptosis upon PCDH-γ depletion in developing chicken spinal neurons. Moreover, overexpression of PDCD10 is sufficient to induce neuronal apoptosis. Taken together, our data reveal a novel function for PDCD10/CCM3, acting as a critical regulator of neuronal survival during development.

  8. 26 CFR 1.706-2T - Temporary regulations; question and answer under the Tax Reform Act of 1984.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Partners and Partnerships § 1.706-2T Temporary regulations; question and answer under the Tax Reform Act of 1984. Question...

  9. Report: EPA Relying on Existing Clean Air Act Regulations to Reduce Atmospheric Deposition to the Chesapeake Bay and its Watershed

    EPA Pesticide Factsheets

    Report #2007-P-00009, February 28, 2007. EPA’s Chesapeake Bay Program Office is relying on anticipated nitrogen deposition reductions from Clean Air Act (CAA) regulations already issued by EPA, combined with other non-air sources' anticipated reductions.

  10. 75 FR 44163 - Implementation of Regulations Required Under Title XI of the Food, Conservation and Energy Act of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-28

    ...-AB07 Implementation of Regulations Required Under Title XI of the Food, Conservation and Energy Act of... clarify conditions for industry compliance with the P&S Act and provide for a fairer market place. DATES... fairer market place. We have received comments asking for an extension of the comment period, and...

  11. Knowledge of the animal welfare act and animal welfare regulations influences attitudes toward animal research.

    PubMed

    Metzger, Mitchell M

    2015-01-01

    Recent public-opinion polls indicate that Americans have shown a decline in support for animal experimentation, and several reports suggest a relationship between people's knowledge of animal welfare regulations and their attitudes toward animal research. Therefore, this study was designed to assess respondent's knowledge of several provisions in the Animal Welfare Act (AWA) and Animal Welfare Regulations (AWR), and determine whether exposure to elements of this legislation would influence an individual's attitudes toward the use of animals in research. A survey was used to assess knowledge of animal research regulations and attitudes toward animal research from a sample of individuals recruited through Amazon's Mechanical Turk crowdsourcing marketplace. Results from study 1 confirmed the hypothesis that respondents had little knowledge of various federal regulations that govern animal research activities. Data from study 2 revealed that exposure to elements of the AWA and AWR influenced participants' attitudes toward the use of animals in research. These results suggest that providing information to the general public about the AWA and AWR that protect laboratory animals from abuse and neglect may help alleviate concerns about using animals in research settings.

  12. FOXA1 acts upstream of GATA2 and AR in hormonal regulation of gene expression

    PubMed Central

    Zhao, Jonathan C.; Fong, Ka-Wing; Jin, Hong-Jian; Yang, Yeqing A; Kim, Jung; Yu, Jindan

    2016-01-01

    Hormonal regulation of gene expression by androgen receptor (AR) is tightly controlled by many transcriptional cofactors, including pioneer factors FOXA1 and GATA2, which, however, exhibit distinct expression patterns and functional roles in prostate cancer. Here, we examined how FOXA1, GATA2, and AR crosstalk and regulate hormone-dependent gene expression in prostate cancer cells. ChIP-seq analysis revealed that FOXA1 reprograms both AR and GATA2 cistrome by preferably recruiting them to FKHD-containing genomic sites. By contrast, GATA2 is unable to shift AR or FOXA1 to GATA motifs. Rather, GATA2 co-occupancy enhances AR and FOXA1 binding to nearby ARE and FKHD sites, respectively. Similarly, AR increases, but not re-programs, GATA2 and FOXA1 cistromes. Concordantly, GATA2 and AR strongly enhance the transcriptional program of each other, whereas FOXA1 regulates GATA2- and AR-mediated gene expression in a context-dependent manner due to its reprogramming effects. Taken together, our data delineated for the first time the distinct mechanisms by which GATA2 and FOXA1 regulate AR cistrome and suggest that FOXA1 acts upstream of GATA2 and AR in determining hormone-dependent gene expression in prostate cancer. PMID:26751772

  13. FOXA1 acts upstream of GATA2 and AR in hormonal regulation of gene expression.

    PubMed

    Zhao, J C; Fong, K-W; Jin, H-J; Yang, Y A; Kim, J; Yu, J

    2016-08-18

    Hormonal regulation of gene expression by androgen receptor (AR) is tightly controlled by many transcriptional cofactors, including pioneer factors FOXA1 and GATA2, which, however, exhibit distinct expression patterns and functional roles in prostate cancer. Here, we examined how FOXA1, GATA2 and AR crosstalk and regulate hormone-dependent gene expression in prostate cancer cells. Chromatin immunoprecipitation sequencing analysis revealed that FOXA1 reprograms both AR and GATA2 cistrome by preferably recruiting them to FKHD-containing genomic sites. By contrast, GATA2 is unable to shift AR or FOXA1 to GATA motifs. Rather, GATA2 co-occupancy enhances AR and FOXA1 binding to nearby ARE and FKHD sites, respectively. Similarly, AR increases, but not reprograms, GATA2 and FOXA1 cistromes. Concordantly, GATA2 and AR strongly enhance the transcriptional program of each other, whereas FOXA1 regulates GATA2- and AR-mediated gene expression in a context-dependent manner due to its reprogramming effects. Taken together, our data delineated for the first time the distinct mechanisms by which GATA2 and FOXA1 regulate AR cistrome and suggest that FOXA1 acts upstream of GATA2 and AR in determining hormone-dependent gene expression in prostate cancer.

  14. Global regulation of alternative splicing by adenosine deaminase acting on RNA (ADAR).

    PubMed

    Solomon, Oz; Oren, Shirley; Safran, Michal; Deshet-Unger, Naamit; Akiva, Pinchas; Jacob-Hirsch, Jasmine; Cesarkas, Karen; Kabesa, Reut; Amariglio, Ninette; Unger, Ron; Rechavi, Gideon; Eyal, Eran

    2013-05-01

    Alternative mRNA splicing is a major mechanism for gene regulation and transcriptome diversity. Despite the extent of the phenomenon, the regulation and specificity of the splicing machinery are only partially understood. Adenosine-to-inosine (A-to-I) RNA editing of pre-mRNA by ADAR enzymes has been linked to splicing regulation in several cases. Here we used bioinformatics approaches, RNA-seq and exon-specific microarray of ADAR knockdown cells to globally examine how ADAR and its A-to-I RNA editing activity influence alternative mRNA splicing. Although A-to-I RNA editing only rarely targets canonical splicing acceptor, donor, and branch sites, it was found to affect splicing regulatory elements (SREs) within exons. Cassette exons were found to be significantly enriched with A-to-I RNA editing sites compared with constitutive exons. RNA-seq and exon-specific microarray revealed that ADAR knockdown in hepatocarcinoma and myelogenous leukemia cell lines leads to global changes in gene expression, with hundreds of genes changing their splicing patterns in both cell lines. This global change in splicing pattern cannot be explained by putative editing sites alone. Genes showing significant changes in their splicing pattern are frequently involved in RNA processing and splicing activity. Analysis of recently published RNA-seq data from glioblastoma cell lines showed similar results. Our global analysis reveals that ADAR plays a major role in splicing regulation. Although direct editing of the splicing motifs does occur, we suggest it is not likely to be the primary mechanism for ADAR-mediated regulation of alternative splicing. Rather, this regulation is achieved by modulating trans-acting factors involved in the splicing machinery.

  15. ATR acts stage specifically to regulate multiple aspects of mammalian meiotic silencing.

    PubMed

    Royo, Hélène; Prosser, Haydn; Ruzankina, Yaroslava; Mahadevaiah, Shantha K; Cloutier, Jeffrey M; Baumann, Marek; Fukuda, Tomoyuki; Höög, Christer; Tóth, Attila; de Rooij, Dirk G; Bradley, Allan; Brown, Eric J; Turner, James M A

    2013-07-01

    In mammals, homologs that fail to synapse during meiosis are transcriptionally inactivated. This process, meiotic silencing, drives inactivation of the heterologous XY bivalent in male germ cells (meiotic sex chromosome inactivation [MSCI]) and is thought to act as a meiotic surveillance mechanism. The checkpoint protein ATM and Rad3-related (ATR) localizes to unsynapsed chromosomes, but its role in the initiation and maintenance of meiotic silencing is unknown. Here we show that ATR has multiple roles in silencing. ATR first regulates HORMA (Hop1, Rev7, and Mad2) domain protein HORMAD1/2 phosphorylation and localization of breast cancer I (BRCA1) and ATR cofactors ATR-interacting peptide (ATRIP)/topoisomerase 2-binding protein 1 (TOPBP1) at unsynapsed axes. Later, it acts as an adaptor, transducing signaling at unsynapsed axes into surrounding chromatin in a manner that requires interdependence with mediator of DNA damage checkpoint 1 (MDC1) and H2AFX. Finally, ATR catalyzes histone H2AFX phosphorylation, the epigenetic event leading to gene inactivation. Using a novel genetic strategy in which MSCI is used to silence a chosen gene in pachytene, we show that ATR depletion does not disrupt the maintenance of silencing and that silencing comprises two phases: The first is dynamic and reversible, and the second is stable and irreversible. Our work identifies a role for ATR in the epigenetic regulation of gene expression and presents a new technique for ablating gene function in the germline.

  16. Confinement regulates complex biochemical networks: initiation of blood clotting by "diffusion acting".

    PubMed

    Shen, Feng; Pompano, Rebecca R; Kastrup, Christian J; Ismagilov, Rustem F

    2009-10-21

    This study shows that environmental confinement strongly affects the activation of nonlinear reaction networks, such as blood coagulation (clotting), by small quantities of activators. Blood coagulation is sensitive to the local concentration of soluble activators, initiating only when the activators surpass a threshold concentration, and therefore is regulated by mass transport phenomena such as flow and diffusion. Here, diffusion was limited by decreasing the size of microfluidic chambers, and it was found that microparticles carrying either the classical stimulus, tissue factor, or a bacterial stimulus, Bacillus cereus, initiated coagulation of human platelet-poor plasma only when confined. A simple analytical argument and numerical model were used to describe the mechanism for this phenomenon: confinement causes diffusible activators to accumulate locally and surpass the threshold concentration. To interpret the results, a dimensionless confinement number, Cn, was used to describe whether a stimulus was confined, and a Damköhler number, Da(2), was used to describe whether a subthreshold stimulus could initiate coagulation. In the context of initiation of coagulation by bacteria, this mechanism can be thought of as "diffusion acting", which is distinct from "diffusion sensing". The ability of confinement and diffusion acting to change the outcome of coagulation suggests that confinement should also regulate other biological "on" and "off" processes that are controlled by thresholds.

  17. The effect of clinical judgment in decision-making: the Mental Health Act 1986 (Vic.) and the Mental Health Review Board.

    PubMed

    Pearson, Megan

    2006-01-01

    This article seeks to critically examine whether the reliance upon clinical judgment in decision-making under the Mental Health Act 1986 (Vic.) (MHA) and the Mental Health Review Board (MHRB) assists or hinders Parliament's key objective in passing the current MHA in 1986: least restrictive treatment.

  18. Application of decision-making theory to the regulation of muscular work rate during self-paced competitive endurance activity.

    PubMed

    Renfree, Andrew; Martin, Louise; Micklewright, Dominic; St Clair Gibson, Alan

    2014-02-01

    Successful participation in competitive endurance activities requires continual regulation of muscular work rate in order to maximise physiological performance capacities, meaning that individuals must make numerous decisions with regards to the muscular work rate selected at any point in time. Decisions relating to the setting of appropriate goals and the overall strategic approach to be utilised are made prior to the commencement of an event, whereas tactical decisions are made during the event itself. This review examines current theories of decision-making in an attempt to explain the manner in which regulation of muscular work is achieved during athletic activity. We describe rational and heuristic theories, and relate these to current models of regulatory processes during self-paced exercise in an attempt to explain observations made in both laboratory and competitive environments. Additionally, we use rational and heuristic theories in an attempt to explain the influence of the presence of direct competitors on the quality of the decisions made during these activities. We hypothesise that although both rational and heuristic models can plausibly explain many observed behaviours in competitive endurance activities, the complexity of the environment in which such activities occur would imply that effective rational decision-making is unlikely. However, at present, many proposed models of the regulatory process share similarities with rational models. We suggest enhanced understanding of the decision-making process during self-paced activities is crucial in order to improve the ability to understand regulation of performance and performance outcomes during athletic activity.

  19. Acting Decisively: China's Story

    ERIC Educational Resources Information Center

    Kohn, Gerald W.; Harvey, James J.

    2009-01-01

    During a 10-day visit to China last year, 25 American superintendents observed a global giant in pursuit of educational excellence. In this article, two of the travelers reflect on what they learned from their experience and what it could mean for Americans. They saw a profound respect for education and pride in learning. They also found support…

  20. MecA Protein Acts as a Negative Regulator of Genetic Competence in Streptococcus mutans

    PubMed Central

    Tian, Xiao-Lin; Dong, Gaofeng; Liu, Tianlei; Gomez, Zubelda A.; Wahl, Astrid; Hols, Pascal

    2013-01-01

    Streptococcus mutans develops competence for genetic transformation through a complex network that receives inputs from at least two signaling peptides, competence-stimulating peptide (CSP) and sigX-inducing peptide (XIP). The key step of competence induction is the transcriptional activation of comX, which encodes an alternative sigma factor, SigX (σX), controlling the expression of late competence genes essential for DNA uptake and recombination. In this study, we provide evidence that MecA acts as a negative regulator in the posttranslational regulation of SigX in S. mutans. Using luxAB transcriptional reporter strains, we demonstrate that MecA represses the expression of late competence genes in S. mutans grown in a complex medium that is subpermissive for competence induction by CSP. The negative regulation of competence by MecA requires the presence of a functional SigX. Accordingly, inactivation of MecA results in a prolonged competence state of S. mutans under this condition. We have also found that the AAA+ protease ClpC displays a similar repressing effect on late competence genes, suggesting that both MecA and ClpC function coordinately to regulate competence in the same regulatory circuit in S. mutans. This suggestion is strongly supported by the results of bacterial two-hybrid assays, which demonstrate that MecA interacts with both SigX and ClpC, forming a ternary SigX-MecA-ClpC complex. Western blot analysis also confirms that inactivation of MecA or ClpC results in the intracellular accumulation of the SigX in S. mutans. Together, our data support the notion that MecA mediates the formation of a ternary SigX-MecA-ClpC complex that sequesters SigX and thereby negatively regulates genetic competence in S. mutans. PMID:24039267

  1. 46 CFR 391.1 - Scope of section 607 of the Act and the regulations in this part.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Scope of section 607 of the Act and the regulations in this part. 391.1 Section 391.1 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION REGULATIONS UNDER PUBLIC LAW 91-469 FEDERAL INCOME TAX ASPECTS OF THE CAPITAL CONSTRUCTION FUND § 391.1...

  2. Epithelial IL-1R2 acts as a homeostatic regulator during remission of ulcerative colitis

    PubMed Central

    Mora-Buch, R; Dotti, I; Planell, N; Calderón-Gómez, E; Jung, P; Masamunt, M C; Llach, J; Ricart, E; Batlle, E; Panés, J; Salas, A

    2016-01-01

    Ulcerative colitis (UC) is a chronic intestinal inflammatory disease that may undergo periods of activity followed by remission. We aimed to identify the endogenous regulatory mechanisms that may promote disease remission. Transcriptional and protein analysis of the intestinal mucosa revealed that the IL-1 decoy receptor, interleukin-1 receptor type 2 (IL1R2), was upregulated in remission compared with active UC and controls. We identified epithelial cells as being responsible for increased IL-1R2 production during remission. Expression of IL1R2 was negatively regulated by Wnt/beta-catenin signals in colonic crypts or epithelial stem cell cultures; accordingly, epithelial stem cells upregulated IL-1R2 upon differentiation. Blocking IL-1R2 in isolated colonic crypt cultures of UC patients in remission and T-cell cultures stimulated with biopsy supernatant from UC patients in remission boosted IL-1β-dependent production of inflammation-related cytokines. Finally, IL1R2 transcription was significantly lower in patients that relapsed during a 1-year follow-up period compared with those in endoscopic remission. Collectively, our results reveal that the IL-1/IL-1R2 axis is differentially regulated in the remitting intestinal mucosa of UC patients. We hypothesize that IL-1R2 in the presence of low concentrations of IL-1β may act locally as a regulator of intestinal homeostasis. PMID:26530134

  3. Abelson kinase acts as a robust, multifunctional scaffold in regulating embryonic morphogenesis

    PubMed Central

    Rogers, Edward M.; Spracklen, Andrew J.; Bilancia, Colleen G.; Sumigray, Kaelyn D.; Allred, S. Colby; Nowotarski, Stephanie H.; Schaefer, Kristina N.; Ritchie, Benjamin J.; Peifer, Mark

    2016-01-01

    Abelson family kinases (Abls) are key regulators of cell behavior and the cytoskeleton during development and in leukemia. Abl’s SH3, SH2, and tyrosine kinase domains are joined via a linker to an F-actin–binding domain (FABD). Research on Abl’s roles in cell culture led to several hypotheses for its mechanism of action: 1) Abl phosphorylates other proteins, modulating their activity, 2) Abl directly regulates the cytoskeleton via its cytoskeletal interaction domains, and/or 3) Abl is a scaffold for a signaling complex. The importance of these roles during normal development remains untested. We tested these mechanistic hypotheses during Drosophila morphogenesis using a series of mutants to examine Abl’s many cell biological roles. Strikingly, Abl lacking the FABD fully rescued morphogenesis, cell shape change, actin regulation, and viability, whereas kinase-dead Abl, although reduced in function, retained substantial rescuing ability in some but not all Abl functions. We also tested the function of four conserved motifs in the linker region, revealing a key role for a conserved PXXP motif known to bind Crk and Abi. We propose that Abl acts as a robust multidomain scaffold with different protein motifs and activities contributing differentially to diverse cellular behaviors. PMID:27385341

  4. Regulating outdoor advertisement boards; employing spatial decision support system to control urban visual pollution

    NASA Astrophysics Data System (ADS)

    Wakil, K.; Hussnain, MQ; Tahir, A.; Naeem, M. A.

    2016-06-01

    Unmanaged placement, size, location, structure and contents of outdoor advertisement boards have resulted in severe urban visual pollution and deterioration of the socio-physical living environment in urban centres of Pakistan. As per the regulatory instruments, the approval decision for a new advertisement installation is supposed to be based on the locational density of existing boards and their proximity or remoteness to certain land- uses. In cities, where regulatory tools for the control of advertisement boards exist, responsible authorities are handicapped in effective implementation due to the absence of geospatial analysis capacity. This study presents the development of a spatial decision support system (SDSS) for regularization of advertisement boards in terms of their location and placement. The knowledge module of the proposed SDSS is based on provisions and restrictions prescribed in regulatory documents. While the user interface allows visualization and scenario evaluation to understand if the new board will affect existing linear density on a particular road and if it violates any buffer restrictions around a particular land use. Technically the structure of the proposed SDSS is a web-based solution which includes open geospatial tools such as OpenGeo Suite, GeoExt, PostgreSQL, and PHP. It uses three key data sets including road network, locations of existing billboards and building parcels with land use information to perform the analysis. Locational suitability has been calculated using pairwise comparison through analytical hierarchy process (AHP) and weighted linear combination (WLC). Our results indicate that open geospatial tools can be helpful in developing an SDSS which can assist solving space related iterative decision challenges on outdoor advertisements. Employing such a system will result in effective implementation of regulations resulting in visual harmony and aesthetic improvement in urban communities.

  5. 75 FR 51420 - Removing Regulations Implementing the Fish and Wildlife Conservation Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-20

    ... Wildlife Conservation Act AGENCY: Fish and Wildlife Service, Interior. ACTION: Final rule. SUMMARY: We, the... Wildlife Conservation Act of 1980. The Act authorized financial and technical assistance to States to... Wildlife Conservation Act of 1980 (16 U.S.C. 2901-2911). This act authorized the Service to give...

  6. atonal regulates neurite arborization but does not act as a proneural gene in the Drosophila brain

    NASA Technical Reports Server (NTRS)

    Hassan, B. A.; Bermingham, N. A.; He, Y.; Sun, Y.; Jan, Y. N.; Zoghbi, H. Y.; Bellen, H. J.

    2000-01-01

    Drosophila atonal (ato) is the proneural gene of the chordotonal organs (CHOs) in the peripheral nervous system (PNS) and the larval and adult photoreceptor organs. Here, we show that ato is expressed at multiple stages during the development of a lineage of central brain neurons that innervate the optic lobes and are required for eclosion. A novel fate mapping approach shows that ato is expressed in the embryonic precursors of these neurons and that its expression is reactivated in third instar larvae (L3). In contrast to its function in the PNS, ato does not act as a proneural gene in the embryonic brain. Instead, ato performs a novel function, regulating arborization during larval and pupal development by interacting with Notch.

  7. An expectancy-value model of emotion regulation: implications for motivation, emotional experience, and decision making.

    PubMed

    Tamir, Maya; Bigman, Yochanan E; Rhodes, Emily; Salerno, James; Schreier, Jenna

    2015-02-01

    According to expectancy-value models of self-regulation, people are motivated to act in ways they expect to be useful to them. For instance, people are motivated to run when they believe running is useful, even when they have nothing to run away from. Similarly, we propose an expectancy-value model of emotion regulation, according to which people are motivated to emote in ways they expect to be useful to them, regardless of immediate contextual demands. For instance, people may be motivated to get angry when they believe anger is useful, even when there is nothing to be angry about. In 5 studies, we demonstrate that leading people to expect an emotion to be useful increased their motivation to experience that emotion (Studies 1-5), led them to up-regulate the experience of that emotion (Studies 3-4), and led to emotion-consistent behavior (Study 4). Our hypotheses were supported when we manipulated the expected value of anxiety (Study 1) and anger (Studies 2-5), both consciously (Studies 1-4) and unconsciously (Study 5). We discuss the theoretical and pragmatic implications of the proposed model.

  8. Enhancer of zeste acts as a major developmental regulator of Ciona intestinalis embryogenesis.

    PubMed

    Le Goff, Emilie; Martinand-Mari, Camille; Martin, Marianne; Feuillard, Jérôme; Boublik, Yvan; Godefroy, Nelly; Mangeat, Paul; Baghdiguian, Stephen; Cavalli, Giacomo

    2015-08-14

    The paradigm of developmental regulation by Polycomb group (PcG) proteins posits that they maintain silencing outside the spatial expression domains of their target genes, particularly of Hox genes, starting from mid embryogenesis. The Enhancer of zeste [E(z)] PcG protein is the catalytic subunit of the PRC2 complex, which silences its targets via deposition of the H3K27me3 mark. Here, we studied the ascidian Ciona intestinalis counterpart of E(z). Ci-E(z) is detected by immunohistochemistry as soon as the 2- and 4-cell stages as a cytoplasmic form and becomes exclusively nuclear thereafter, whereas the H3K27me3 mark is detected starting from the gastrula stage and later. Morpholino invalidation of Ci-E(z) leads to the total disappearance of both Ci-E(z) protein and its H3K27me3 mark. Ci-E(z) morphants display a severe phenotype. Strikingly, the earliest defects occur at the 4-cell stage with the dysregulation of cell positioning and mitotic impairment. At later stages, Ci-E(z)-deficient embryos are affected by terminal differentiation defects of neural, epidermal and muscle tissues, by the failure to form a notochord and by the absence of caudal nerve. These major phenotypic defects are specifically rescued by injection of a morpholino-resistant Ci-E(z) mRNA, which restores expression of Ci-E(z) protein and re-deposition of the H3K27me3 mark. As observed by qPCR analyses, Ci-E(z) invalidation leads to the early derepression of tissue-specific developmental genes, whereas late-acting developmental genes are generally down-regulated. Altogether, our results suggest that Ci-E(z) plays a major role during embryonic development in Ciona intestinalis by silencing early-acting developmental genes in a Hox-independent manner.

  9. Extracellular calcium acts as a “third messenger” to regulate enzyme and alkaline secretion

    PubMed Central

    Caroppo, Rosa; Gerbino, Andrea; Fistetto, Gregorio; Colella, Matilde; Debellis, Lucantonio; Hofer, Aldebaran M.; Curci, Silvana

    2004-01-01

    It is generally assumed that the functional consequences of stimulation with Ca2+-mobilizing agonists are derived exclusively from the second messenger action of intracellular Ca2+, acting on targets inside the cells. However, during Ca2+ signaling events, Ca2+ moves in and out of the cell, causing changes not only in intracellular Ca2+, but also in local extracellular Ca2+. The fact that numerous cell types possess an extracellular Ca2+ “sensor” raises the question of whether these dynamic changes in external [Ca2+] may serve some sort of messenger function. We found that in intact gastric mucosa, the changes in extracellular [Ca2+] secondary to carbachol-induced increases in intracellular [Ca2+] were sufficient and necessary to elicit alkaline secretion and pepsinogen secretion, independent of intracellular [Ca2+] changes. These findings suggest that extracellular Ca2+ can act as a “third messenger” via Ca2+ sensor(s) to regulate specific subsets of tissue function previously assumed to be under the direct control of intracellular Ca2+. PMID:15240573

  10. 34 CFR 100.10 - Decisions and notices.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... TITLE VI OF THE CIVIL RIGHTS ACT OF 1964 § 100.10 Decisions and notices. (a) Decisions by hearing... section shall remain in effect. (Authority: Sec. 602, Civil Rights Act of 1964; 78 Stat. 252; 42 U.S.C... Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF...

  11. 34 CFR 100.10 - Decisions and notices.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... TITLE VI OF THE CIVIL RIGHTS ACT OF 1964 § 100.10 Decisions and notices. (a) Decisions by hearing... section shall remain in effect. (Authority: Sec. 602, Civil Rights Act of 1964; 78 Stat. 252; 42 U.S.C... Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF...

  12. 34 CFR 100.10 - Decisions and notices.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... TITLE VI OF THE CIVIL RIGHTS ACT OF 1964 § 100.10 Decisions and notices. (a) Decisions by hearing... section shall remain in effect. (Authority: Sec. 602, Civil Rights Act of 1964; 78 Stat. 252; 42 U.S.C... Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF...

  13. 34 CFR 100.10 - Decisions and notices.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... TITLE VI OF THE CIVIL RIGHTS ACT OF 1964 § 100.10 Decisions and notices. (a) Decisions by hearing... section shall remain in effect. (Authority: Sec. 602, Civil Rights Act of 1964; 78 Stat. 252; 42 U.S.C... Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF...

  14. 34 CFR 100.10 - Decisions and notices.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... TITLE VI OF THE CIVIL RIGHTS ACT OF 1964 § 100.10 Decisions and notices. (a) Decisions by hearing... section shall remain in effect. (Authority: Sec. 602, Civil Rights Act of 1964; 78 Stat. 252; 42 U.S.C... Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF...

  15. Decision-making in product portfolios of pharmaceutical research and development – managing streams of innovation in highly regulated markets

    PubMed Central

    Jekunen, Antti

    2014-01-01

    Decision-making is a core function of any drug development firm. Developing drugs demands a firm to be highly innovative, while at the same time the activity is strictly regulated. Successful drug development offers the right to apply for a long-term patent that confers exclusive marketing rights. This article addresses the issue of what constitutes an adequate portfolio of drugs for a drug development firm and how it might be managed successfully. The paper investigates decision-making in the industry and specifically in the development of oncology drugs from various perspectives: the need for decisions, their timing, decision-making at the project level, the optimal portfolio, tools for portfolio analysis, the evaluation of patents, and finally the importance of the drug portfolio. Drug development decisions as important organizational elements should get more emphasis, and decisions in drug portfolio using modern decision-making methods should be used more widely than what currently happens. Structured, informed decisions would help avoiding late terminations of drugs in Phase III development. An improved research and development pipeline and drug portfolio management are the major elements in the general strategy targeting success. PMID:25364229

  16. Decision-making in product portfolios of pharmaceutical research and development--managing streams of innovation in highly regulated markets.

    PubMed

    Jekunen, Antti

    2014-01-01

    Decision-making is a core function of any drug development firm. Developing drugs demands a firm to be highly innovative, while at the same time the activity is strictly regulated. Successful drug development offers the right to apply for a long-term patent that confers exclusive marketing rights. This article addresses the issue of what constitutes an adequate portfolio of drugs for a drug development firm and how it might be managed successfully. The paper investigates decision-making in the industry and specifically in the development of oncology drugs from various perspectives: the need for decisions, their timing, decision-making at the project level, the optimal portfolio, tools for portfolio analysis, the evaluation of patents, and finally the importance of the drug portfolio. Drug development decisions as important organizational elements should get more emphasis, and decisions in drug portfolio using modern decision-making methods should be used more widely than what currently happens. Structured, informed decisions would help avoiding late terminations of drugs in Phase III development. An improved research and development pipeline and drug portfolio management are the major elements in the general strategy targeting success.

  17. Reiterative changes in the Italian regulation on IVF: the effect on PGD patients' reproductive decisions.

    PubMed

    Gianaroli, Luca; Crivello, Anna Maria; Stanghellini, Ilaria; Ferraretti, Anna Pia; Tabanelli, Carla; Magli, Maria Cristina

    2014-01-01

    National legislations represent one of the main factors influencing access to assisted reproduction treatment. The Italian situation in the last decade is an example of how the treatment of patients for preimplantation genetic diagnosis (PGD) was more dependent on regulators than on medical choices. This report analysed how the changes in Italian regulation affected the number of PGD referrals to this study centre, as well as their decision to opt for cross-border reproductive care (CBRC). The analysis showed that during the period in which PGD was actually not performed because of the restriction imposed by the Italian law on IVF (from 24 February 2004 to 7 May 2009) there was a significant decrease in the number of referrals asking for PGD (2.5% of total referrals) compared with the previous years (3.3%; P < 0.025) and following years when PGD was legalized (5.1%; P < 0.001). The number of couples opting for CBRC had an opposite trend, reaching a maximum when PGD was banned from Italian centres (55 couples), whereas after the readmission of PGD, only eight couples went abroad for treatment. Concomitantly, since May 2009, the proportion of couples performing a PGD cycle in this centre has constantly increased.

  18. Polycomb protein EZH2 regulates cancer cell fate decision in response to DNA damage.

    PubMed

    Wu, Z; Lee, S T; Qiao, Y; Li, Z; Lee, P L; Lee, Y J; Jiang, X; Tan, J; Aau, M; Lim, C Z H; Yu, Q

    2011-11-01

    Polycomb protein histone methyltransferase enhancer of Zeste homologe 2 (EZH2) is frequently overexpressed in human malignancy and is implicated in cancer cell proliferation and invasion. However, it is largely unknown whether EZH2 has a role in modulating DNA damage response. Here, we show that EZH2 is an important determinant of cell fate decision in response to genotoxic stress. EZH2 depletion results in abrogation of both cell cycle G1 and G2/M checkpoints, directing DNA damage response toward predominant apoptosis in both p53-proficient and p53-deficient cancer cells, but not in normal cells. Mechanistically, EZH2 regulates DNA damage response in p53 wild-type cells mainly through transcriptional repression of FBXO32, which binds to and directs p21 for proteasome-mediated degradation, whereas it affects p53-deficient cells through regulating Chk1 activation by a distinct mechanism. Furthermore, pharmacological depletion of EZH2 phenocopies the effects of EZH2 knockdown on cell cycle checkpoints and apoptosis. These data unravel a crucial role of EZH2 in determining the cancer cell outcome following DNA damage and suggest that therapeutic targeting oncogenic EZH2 might serve as a strategy for improving conventional chemotherapy in a given malignancy.

  19. Protein kinase CK2: structure, regulation and role in cellular decisions of life and death.

    PubMed Central

    Litchfield, David W

    2003-01-01

    Protein kinase CK2 ('casein kinase II') has traditionally been classified as a messenger-independent protein serine/threonine kinase that is typically found in tetrameric complexes consisting of two catalytic (alpha and/or alpha') subunits and two regulatory beta subunits. Accumulated biochemical and genetic evidence indicates that CK2 has a vast array of candidate physiological targets and participates in a complex series of cellular functions, including the maintenance of cell viability. This review summarizes current knowledge of the structural and enzymic features of CK2, and discusses advances that challenge traditional views of this enzyme. For example, the recent demonstrations that individual CK2 subunits exist outside tetrameric complexes and that CK2 displays dual-specificity kinase activity raises new prospects for the precise elucidation of its regulation and cellular functions. This review also discusses a number of the mechanisms that contribute to the regulation of CK2 in cells, and will highlight emerging insights into the role of CK2 in cellular decisions of life and death. In this latter respect, recent evidence suggests that CK2 can exert an anti-apoptotic role by protecting regulatory proteins from caspase-mediated degradation. The mechanistic basis of the observation that CK2 is essential for viability may reside in part in this ability to protect cellular proteins from caspase action. Furthermore, this anti-apoptotic function of CK2 may contribute to its ability to participate in transformation and tumorigenesis. PMID:12396231

  20. Testing decision rules for categorizing species' extinction risk to help develop quantitative listing criteria for the U.S. Endangered Species Act.

    PubMed

    Regan, Tracey J; Taylor, Barbara L; Thompson, Grant G; Cochrane, Jean Fitts; Ralls, Katherine; Runge, Michael C; Merrick, Richard

    2013-08-01

    Lack of guidance for interpreting the definitions of endangered and threatened in the U.S. Endangered Species Act (ESA) has resulted in case-by-case decision making leaving the process vulnerable to being considered arbitrary or capricious. Adopting quantitative decision rules would remedy this but requires the agency to specify the relative urgency concerning extinction events over time, cutoff risk values corresponding to different levels of protection, and the importance given to different types of listing errors. We tested the performance of 3 sets of decision rules that use alternative functions for weighting the relative urgency of future extinction events: a threshold rule set, which uses a decision rule of x% probability of extinction over y years; a concave rule set, where the relative importance of future extinction events declines exponentially over time; and a shoulder rule set that uses a sigmoid shape function, where relative importance declines slowly at first and then more rapidly. We obtained decision cutoffs by interviewing several biologists and then emulated the listing process with simulations that covered a range of extinction risks typical of ESA listing decisions. We evaluated performance of the decision rules under different data quantities and qualities on the basis of the relative importance of misclassification errors. Although there was little difference between the performance of alternative decision rules for correct listings, the distribution of misclassifications differed depending on the function used. Misclassifications for the threshold and concave listing criteria resulted in more overprotection errors, particularly as uncertainty increased, whereas errors for the shoulder listing criteria were more symmetrical. We developed and tested the framework for quantitative decision rules for listing species under the U.S. ESA. If policy values can be agreed on, use of this framework would improve the implementation of the ESA by

  1. Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis

    NASA Technical Reports Server (NTRS)

    Brown, D. E.; Rashotte, A. M.; Murphy, A. S.; Normanly, J.; Tague, B. W.; Peer, W. A.; Taiz, L.; Muday, G. K.

    2001-01-01

    Polar transport of the plant hormone auxin controls many aspects of plant growth and development. A number of synthetic compounds have been shown to block the process of auxin transport by inhibition of the auxin efflux carrier complex. These synthetic auxin transport inhibitors may act by mimicking endogenous molecules. Flavonoids, a class of secondary plant metabolic compounds, have been suggested to be auxin transport inhibitors based on their in vitro activity. The hypothesis that flavonoids regulate auxin transport in vivo was tested in Arabidopsis by comparing wild-type (WT) and transparent testa (tt4) plants with a mutation in the gene encoding the first enzyme in flavonoid biosynthesis, chalcone synthase. In a comparison between tt4 and WT plants, phenotypic differences were observed, including three times as many secondary inflorescence stems, reduced plant height, decreased stem diameter, and increased secondary root development. Growth of WT Arabidopsis plants on naringenin, a biosynthetic precursor to those flavonoids with auxin transport inhibitor activity in vitro, leads to a reduction in root growth and gravitropism, similar to the effects of synthetic auxin transport inhibitors. Analyses of auxin transport in the inflorescence and hypocotyl of independent tt4 alleles indicate that auxin transport is elevated in plants with a tt4 mutation. In hypocotyls of tt4, this elevated transport is reversed when flavonoids are synthesized by growth of plants on the flavonoid precursor, naringenin. These results are consistent with a role for flavonoids as endogenous regulators of auxin transport.

  2. 43 CFR 10010.50 - Implementing the decision.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... POLICY ACT Relationship to Decision-Making § 10010.50 Implementing the decision. The terms “monitoring... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Implementing the decision. 10010.50 Section 10010.50 Public Lands: Interior Regulations Relating to Public Lands (Continued) UTAH...

  3. 43 CFR 10010.50 - Implementing the decision.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... POLICY ACT Relationship to Decision-Making § 10010.50 Implementing the decision. The terms “monitoring... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Implementing the decision. 10010.50 Section 10010.50 Public Lands: Interior Regulations Relating to Public Lands (Continued) UTAH...

  4. 43 CFR 10010.50 - Implementing the decision.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... POLICY ACT Relationship to Decision-Making § 10010.50 Implementing the decision. The terms “monitoring... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Implementing the decision. 10010.50 Section 10010.50 Public Lands: Interior Regulations Relating to Public Lands (Continued) UTAH...

  5. 43 CFR 10010.50 - Implementing the decision.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... POLICY ACT Relationship to Decision-Making § 10010.50 Implementing the decision. The terms “monitoring... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Implementing the decision. 10010.50 Section 10010.50 Public Lands: Interior Regulations Relating to Public Lands (Continued) UTAH...

  6. 31 CFR 501.740 - Decision of Administrative Law Judge.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Decision of Administrative Law Judge... REGULATIONS Trading With the Enemy Act (TWEA) Penalties § 501.740 Decision of Administrative Law Judge. The Administrative Law Judge shall prepare a decision that constitutes his or her final disposition of...

  7. Allowance trading under the Clean Air Act: Who should regulate, and when?

    SciTech Connect

    Lock, R.

    1993-07-01

    The goal of this paper is to explore how compliance with the Clean Air Act Amendments of 1990 (CAAA), especially Title IV and emission trading under it, will affect the current relationship between state and federal regulation. It is difficult, with the limited experience we have had under Title IV, to be definitive about or to be a very strong advocate of too many policy positions. What may be most helpful at this point is to identify where the difficult issues in state/federal relations might arise; and then to explore ways in which tensions might be either avoided or resolved. One anticipated conclusion is that a traditional regulatory mindset could be very destructive if applied to this new area of oversight without due sensitivity to what Congress is trying to achieve in Title IV. That concern pervaded the early legislative debates; and it persists today. Title IV presents some unique challenges to state regulators and will require some creative solutions and fresh thinking if the goals of Congress are to be realized and the full benefits that allowance trading can offer are to be reaped by electricity consumers. In the ultimate analysis, Title IV amounts to a massive internalization of the external costs imposed on society by acid rain deposition. (This places in serious question the notion of additional externality {open_quotes}adders{close_quotes} for sulfur dioxide (SO{sub 2}) and nitrous oxide (NO{sub x}) at the state level for utility supply planning purposes.) The whole point of Title IV is to give those directly charged with compliance, namely power producers, the maximum flexibility to pursue least-cost compliance solutions. Perhaps the biggest single factor in how well they do this will be how state regulators respond to their compliance and allowance trading initiatives.

  8. Some impacts of the 1990 Clean Air Act and state clean-air regulations on the fertilizer industry

    SciTech Connect

    Breed, C.E.; Kerns, O.S.

    1992-12-01

    The Clean Air Act amendments of 1990 will intensify national efforts to reduce air pollution. They will have major impacts an governmental agencies and on industrial and commercial facilities throughout the country. As with other industries, it is essential for fertilizer dealers and producers to understand how these changes to the Clean Air Act can significantly change the way they do business. This paper is proffered as an overview of ways in which the 1990 amendments to the Clean Air Act may impact the fertilizer industry. The nonattainment, toxics, and permit provisions of the amended act will be three areas of particular concern to the fertilizer industry. Implementation of the new regulatory requirements of this legislation promises to be a long and onerous process for all concerned. However, it appears that state and local regulations may have a much more profound impact on the fertilizer industry than the new Clean Air Act.

  9. Some impacts of the 1990 Clean Air Act and state clean-air regulations on the fertilizer industry

    SciTech Connect

    Breed, C.E.; Kerns, O.S.

    1992-12-31

    The Clean Air Act amendments of 1990 will intensify national efforts to reduce air pollution. They will have major impacts on governmental agencies and on industrial and commercial facilities throughout the country. As with other industries, it is essential for fertilizer dealers and producers to understand how these changes to the Clean Air Act can significantly change the way they do business. This paper is proffered as an overview of ways in which the 1990 amendments to the Clean Air Act may impact the fertilizer industry. The nonattainment, toxics, and permit provisions of the amended act will be three areas of particular concern to the fertilizer industry. Implementation of the new regulatory requirements of this legislation promises to be a long and onerous process for all concerned. However, it appears that state and local regulations may have a much more profound impact on the fertilizer industry than the new Clean Air Act.

  10. 25 CFR 162.597 - Will BIA appeal bond regulations apply to cancellation decisions involving WSR leases?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false Will BIA appeal bond regulations apply to cancellation decisions involving WSR leases? 162.597 Section 162.597 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER LEASES AND PERMITS Wind and Solar Resource Leases Wsr Lease...

  11. 25 CFR 162.597 - Will BIA appeal bond regulations apply to cancellation decisions involving WSR leases?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false Will BIA appeal bond regulations apply to cancellation decisions involving WSR leases? 162.597 Section 162.597 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER LEASES AND PERMITS Wind and Solar Resource Leases Wsr Lease...

  12. Enhancing Decision-Making in STSE Education by Inducing Reflection and Self-Regulated Learning

    ERIC Educational Resources Information Center

    Gresch, Helge; Hasselhorn, Marcus; Bögeholz, Susanne

    2017-01-01

    Thoughtful decision-making to resolve socioscientific issues is central to science, technology, society, and environment (STSE) education. One approach for attaining this goal involves fostering students' decision-making processes. Thus, the present study explores whether the application of decision-making strategies, combined with reflections on…

  13. Retargeting a Dual-Acting sRNA for Multiple mRNA Transcript Regulation.

    PubMed

    Lahiry, Ashwin; Stimple, Samuel D; Wood, David W; Lease, Richard A

    2017-01-24

    Multitargeting small regulatory RNAs (sRNAs) represent a potentially useful tool for metabolic engineering applications. Natural multitargeting sRNAs govern bacterial gene expression by binding to the translation initiation regions of protein-coding mRNAs through base pairing. We designed an Escherichia coli based genetic system to create and assay dual-acting retargeted-sRNA variants. The variants can be assayed for coordinate translational regulation of two alternate mRNA leaders fused to independent reporter genes. Accordingly, we began with the well-characterized E. coli native DsrA sRNA. The merits of using DsrA include its well-characterized separation of function into two independently folded stem-loop domains, wherein alterations at one stem do not necessarily abolish activity at the other stem. Expression of the sRNA and each reporter mRNA was independently controlled by small inducer molecules, allowing precise quantification of the regulatory effects of each sRNA:mRNA interaction in vivo with a microtiter plate assay. Using this system, we semirationally designed DsrA variants screened in E. coli for their ability to regulate key mRNA leader sequences from the Clostridium acetobutylicum n-butanol synthesis pathway. To coordinate intervention at two points in a metabolic pathway, we created bifunctional sRNA prototypes by combining sequences from two singly retargeted DsrA variants. This approach constitutes a platform for designing sRNAs to specifically target arbitrary mRNA transcript sequences, and thus provides a generalizable tool for retargeting and characterizing multitarget sRNAs for metabolic engineering.

  14. Toward a multi-objective decision support framework to support regulations of unconventional oil and gas development

    NASA Astrophysics Data System (ADS)

    Alongi, M.; Howard, C.; Kasprzyk, J. R.; Ryan, J. N.

    2015-12-01

    Unconventional oil and gas development (UOGD) using hydraulic fracturing and horizontal drilling has recently fostered an unprecedented acceleration in energy development. Regulations seek to protect environmental quality of areas surrounding UOGD, while maintaining economic benefits. One such regulation is a setback distance, which dictates the minimum proximity between an oil and gas well and an object such as a residential or commercial building, property line, or water source. In general, most setback regulations have been strongly politically motivated without a clear scientific basis for understanding the relationship between the setback distance and various performance outcomes. This presentation discusses a new decision support framework for setback regulations, as part of a large NSF-funded sustainability research network (SRN) on UOGD. The goal of the decision support framework is to integrate a wide array of scientific information from the SRN into a coherent framework that can help inform policy regarding UOGD. The decision support framework employs multiobjective evolutionary algorithm (MOEA) optimization coupled with simulation models of air quality and other performance-based outcomes on UOGD. The result of the MOEA optimization runs are quantitative tradeoff curves among different objectives. For example, one such curve could demonstrate air pollution concentrations versus estimates of energy development profits, for different levels of setback distance. Our results will also inform policy-relevant discussions surrounding UOGD such as comparing single- and multi-well pads, as well as regulations on the density of well development over a spatial area.

  15. Pyrethroid mode(s) of action in the context of Food Quality Protection Act (FQPA) regulation.

    PubMed

    Gammon, Derek W; Leggett, Michael F; Clark, John M

    2011-04-13

    A Scientific Advisory Panel (SAP) in June 2009 concluded that a common mode of action existed for pyrethroids, with two subgroups. The purpose of this SAP was to advise the U.S. Environmental Protection Agency on the validity of regulation of pyrethroids as a single class under the Food Quality Protection Act of 1996. Two types of pyrethroid action were first described for clinical signs in the rat and clinical signs/nerve effects in the cockroach. In insects, Type I clinical signs correlate with repetitive firing in nerve axons, especially fine sensory axons. The Na(+) inward current is via a TTX-sensitive voltage-gated sodium channel (VGSC). Type II (α-CN) effects on VGSCs do not include repetitive firing following stimulation in these axons. Instead, Type II effects on VGSCs include prolonged Na(+) tail currents along with depolarization of nerve membrane. Other Type II effects have been measured on VG Ca(2+) and K(+) channels and VG and GABA-activated Cl(-) channels. In conclusion, in vivo pyrethroid effects in mammals should be linked with specific channel effects, allowing the use of specific clinical signs or ion channel effects for pyrethroid risk assessment.

  16. Impact of Clean Air Act Regulations on Nitrogen Fate and Transport in the Neuse River Basin

    NASA Astrophysics Data System (ADS)

    Gabriel, M. C.; Knightes, C. D.; Dennis, R. L.; Cooter, E. J.

    2012-12-01

    This study investigated impacts of Clean Air Act Amendment (CAAA) NOx emissions regulations on the fate and transport of nitrogen for two watersheds in the Neuse River Basin, North Carolina, USA from 1990 to 2020. The Soil and Water Assessment Tool (SWAT) and the Community Multi-scale Air Quality (CMAQ) modeling system were used. CMAQ simulated atmospheric chemical transport and nitrogen deposition. This data was entered into SWAT which simulated watershed hydrology and water quality. Two cases were investigated: one that incorporates CAAA regulatory emissions controls in CMAQ simulation (with) and a second case that does not (without). SWAT model results forecasted a 70% decrease in inorganic nitrogen discharge from the Little River watershed and a 50% decrease for the Nahunta watershed by 2020 under the emission control (with) scenario. Denitrification and plant nitrogen uptake played important roles in nitrogen discharge from each watershed. The nitrogen discharge response time following a change in atmospheric nitrogen deposition was 4 years for the Nahunta watershed and 2 years for the Little River watershed. The longer response time for Nahunta is primarily due to a higher percentage of soybean land cover (22.5% [Nahunta]; 1.6% [Little River]). Agricultural land covers had varied nitrogen response times to changes in atmospheric deposition, particularly for soybean, hay and corn. The studied watersheds retained >80% of all nitrogen delivered by agriculture fertilization, biological fixation and atmospheric deposition.

  17. Egr2 induced during DC development acts as an intrinsic negative regulator of DC immunogenicity.

    PubMed

    Miah, Mohammad Alam; Byeon, Se Eun; Ahmed, Md Selim; Yoon, Cheol-Hee; Ha, Sang-Jun; Bae, Yong-Soo

    2013-09-01

    Early growth response gene 2 (Egr2), which encodes a zinc finger transcription factor, is rapidly and transiently induced in various cell types independently of de novo protein synthesis. Although a role for Egr2 is well established in T-cell development, Egr2 expression and its biological function in dendritic cells (DCs) have not yet been described. Here, we demonstrate Egr2 expression during DC development, and its role in DC-mediated immune responses. Egr2 is expressed in the later stage of DC development from BM precursor cells. Even at steady state, Egr2 is highly expressed in mouse splenic DCs. Egr2-knockdown (Egr2-KD) DCs showed increased levels of major histocompatability complex (MHC) class I and II and co-stimulatory molecules, and enhanced antigen uptake and migratory capacities. Furthermore, Egr2-KD abolished SOCS1 expression and signal transducer and activator of transcription 5 (STAT5) activation during DC development, probably resulting in the enhancement of IL-12 expression and Th1 immunogenicity of a DC vaccine. DC-mediated cytotoxic T lymphocyte (CTL) activation and antitumor immunity were significantly enhanced by Egr2-KD, and impaired by Egr2 overexpression in antigen-pulsed DC vaccines. These data suggest that Egr2 acts as an intrinsic negative regulator of DC immunogenicity and can be an attractive molecular target for DC vaccine development.

  18. 78 FR 79729 - Integrated Mortgage Disclosures Under the Real Estate Settlement Procedures Act (Regulation X...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-31

    ...Sections 1098 and 1100A of the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act) direct the Bureau to publish rules and forms that combine certain disclosures that consumers receive in connection with applying for and closing on a mortgage loan under the Truth in Lending Act and the Real Estate Settlement Procedures Act. Consistent with this requirement, the Bureau is......

  19. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES... objective intent; (3) The manufacture of the HCT/P does not involve the combination of the cells or tissues... not have a systemic effect and is not dependent upon the metabolic activity of living cells for...

  20. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES... objective intent; (3) The manufacture of the HCT/P does not involve the combination of the cells or tissues... not have a systemic effect and is not dependent upon the metabolic activity of living cells for...

  1. 76 FR 72878 - Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations-Imposition of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... Act Regulations--Imposition of Special Measure Against the Islamic Republic of Iran as a Jurisdiction... of FinCEN, found that reasonable grounds exist for concluding that the Islamic Republic of Iran...CEN has determined that reasonable grounds exist for concluding that the Islamic Republic of Iran is...

  2. 78 FR 63408 - Petition To Amend Animal Welfare Act Regulations To Prohibit Public Contact With Big Cats, Bears...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-24

    ... Animal Welfare Act Regulations To Prohibit Public Contact With Big Cats, Bears, and Nonhuman Primates... exceptions, from coming into direct or physical contact with big cats, bears, or nonhuman primates of any age... big cats, bears, and nonhuman primates and the separation of such animals from their dams before...

  3. 76 FR 39007 - Native American Graves Protection and Repatriation Act Regulations- Definition of “Indian Tribe”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-05

    ... and Repatriation Act Regulations-- Definition of ``Indian Tribe'' AGENCY: Office of the Secretary... definition of ``Indian tribe,'' because it is inconsistent with the statutory definition of that term. DATES... definition of ``Indian tribe'' into NAGPRA verbatim from the Indian Self Determination and...

  4. 75 FR 29526 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-26

    ... No: 2010-12622] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [131 FERC ] 61,161; Docket No. RM93-11-000] Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods May 19, 2010. The...

  5. 76 FR 28966 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The...

  6. 77 FR 29997 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The...

  7. 78 FR 30296 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The...

  8. 78 FR 30739 - Amendments to the 2013 Escrows Final Rule under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ...) and Homeownership Counseling Amendments to the Real Estate Settlement Procedures Act (Regulation X... Appraisals Final Rule), 78 FR 7215 (Jan. 31, 2013); Mortgage Servicing Rules Under the Real Estate Settlement...). \\13\\ 73 FR 44522 (July 30, 2008). IV. Section-by-Section Analysis Section 1026.35 Requirements...

  9. 9 CFR 331.3 - States designated under paragraph 301(c) of the Act; application of regulations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false States designated under paragraph 301(c) of the Act; application of regulations. 331.3 Section 331.3 Animals and Animal Products FOOD... DESIGNATED STATES AND TERRITORIES; AND FOR DESIGNATION OF ESTABLISHMENTS WHICH ENDANGER PUBLIC HEALTH AND...

  10. 9 CFR 381.222 - States designated under paragraph 5(c) of the Act; application of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false States designated under paragraph 5(c) of the Act; application of regulations. 381.222 Section 381.222 Animals and Animal Products FOOD... Designating Establishments With Operations Which Would Clearly Endanger the Public Health; Disposition...

  11. 9 CFR 331.3 - States designated under paragraph 301(c) of the Act; application of regulations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false States designated under paragraph 301(c) of the Act; application of regulations. 331.3 Section 331.3 Animals and Animal Products FOOD... DESIGNATED STATES AND TERRITORIES; AND FOR DESIGNATION OF ESTABLISHMENTS WHICH ENDANGER PUBLIC HEALTH AND...

  12. 9 CFR 331.3 - States designated under paragraph 301(c) of the Act; application of regulations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false States designated under paragraph 301(c) of the Act; application of regulations. 331.3 Section 331.3 Animals and Animal Products FOOD... DESIGNATED STATES AND TERRITORIES; AND FOR DESIGNATION OF ESTABLISHMENTS WHICH ENDANGER PUBLIC HEALTH AND...

  13. 9 CFR 381.222 - States designated under paragraph 5(c) of the Act; application of regulations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false States designated under paragraph 5(c) of the Act; application of regulations. 381.222 Section 381.222 Animals and Animal Products FOOD... Designating Establishments With Operations Which Would Clearly Endanger the Public Health; Disposition...

  14. 9 CFR 331.3 - States designated under paragraph 301(c) of the Act; application of regulations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false States designated under paragraph 301(c) of the Act; application of regulations. 331.3 Section 331.3 Animals and Animal Products FOOD... DESIGNATED STATES AND TERRITORIES; AND FOR DESIGNATION OF ESTABLISHMENTS WHICH ENDANGER PUBLIC HEALTH AND...

  15. 9 CFR 381.222 - States designated under paragraph 5(c) of the Act; application of regulations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false States designated under paragraph 5(c) of the Act; application of regulations. 381.222 Section 381.222 Animals and Animal Products FOOD... Designating Establishments With Operations Which Would Clearly Endanger the Public Health; Disposition...

  16. 9 CFR 331.3 - States designated under paragraph 301(c) of the Act; application of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false States designated under paragraph 301(c) of the Act; application of regulations. 331.3 Section 331.3 Animals and Animal Products FOOD... DESIGNATED STATES AND TERRITORIES; AND FOR DESIGNATION OF ESTABLISHMENTS WHICH ENDANGER PUBLIC HEALTH AND...

  17. 45 CFR 1703.203 - Decision to close meeting.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....203 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Procedures Governing Decisions About Meetings... public observation a meeting or portion or portions thereof, or to withhold information pertaining...

  18. Employee Retirement Income Security Act of 1974: rules and regulations for administration and enforcement; claims procedure. Pension and Welfare Benefits Administration, Labor. Final regulation.

    PubMed

    2000-11-21

    This document contains a final regulation revising the minimum requirements for benefit claims procedures of employee benefit plans covered by Title I of the Employee Retirement Income Security Act of 1974 (ERISA or the Act). The regulation establishes new standards for the processing of claims under group health plans and plans providing disability benefits and further clarifies existing standards for all other employee benefit plans. The new standards are intended to ensure more timely benefit determinations, to improve access to information on which a benefit determination is made, and to assure that participants and beneficiaries will be afforded a full and fair review of denied claims. When effective, the regulation will affect participants and beneficiaries of employee benefit plans, employers who sponsor employee benefit plans, plan fiduciaries, and others who assist in the provision of plan benefits, such as third-party benefits administrators and health service providers or health maintenance organizations that provide benefits to participants and beneficiaries of employee benefit plans.

  19. Genetic Regulation of Fate Decisions in Therapeutic T Cells to Enhance Tumor Protection and Memory Formation.

    PubMed

    Veliça, Pedro; Zech, Mathias; Henson, Sian; Holler, Angelika; Manzo, Teresa; Pike, Rebecca; Santos E Sousa, Pedro; Zhang, Lei; Heinz, Niels; Schiedlmeier, Bernhard; Pule, Martin; Stauss, Hans; Chakraverty, Ronjon

    2015-07-01

    A key challenge in the field of T-cell immunotherapy for cancer is creating a suitable platform for promoting differentiation of effector cells while at the same time enabling self-renewal needed for long-term memory. Although transfer of less differentiated memory T cells increases efficacy through greater expansion and persistence in vivo, the capacity of such cells to sustain effector functions within immunosuppressive tumor microenvironments may still be limiting. We have therefore directly compared the impact of effector versus memory differentiation of therapeutic T cells in tumor-bearing mice by introducing molecular switches that regulate cell fate decisions via mTOR. Ectopic expression of RAS homolog enriched in brain (RHEB) increased mTORC1 signaling, promoted a switch to aerobic glycolysis, and increased expansion of effector T cells. By rapidly infiltrating tumors, RHEB-transduced T cells significantly reduced the emergence of immunoedited escape variants. In contrast, expression of proline-rich Akt substrate of 40 kDa (PRAS40) inhibited mTORC1, promoted quiescence, and blocked tumor infiltration. Fate mapping studies following transient expression of PRAS40 demonstrated that mTORC1(low) T cells made no contribution to initial tumor control but instead survived to become memory cells proficient in generating recall immunity. Our data support the design of translational strategies for generating heterogeneous T-cell immunity against cancer, with the appropriate balance between promoting effector differentiation and self-renewal. Unlike pharmacologic inhibitors, the genetic approach described here allows for upregulation as well as inhibition of the mTORC1 pathway and is highly selective for the therapeutic T cells without affecting systemic mTORC1 functions.

  20. Auxin Acts through MONOPTEROS to Regulate Plant Cell Polarity and Pattern Phyllotaxis.

    PubMed

    Bhatia, Neha; Bozorg, Behruz; Larsson, André; Ohno, Carolyn; Jönsson, Henrik; Heisler, Marcus G

    2016-12-05

    The periodic formation of plant organs such as leaves and flowers gives rise to intricate patterns that have fascinated biologists and mathematicians alike for hundreds of years [1]. The plant hormone auxin plays a central role in establishing these patterns by promoting organ formation at sites where it accumulates due to its polar, cell-to-cell transport [2-6]. Although experimental evidence as well as modeling suggest that feedback from auxin to its transport direction may help specify phyllotactic patterns [7-12], the nature of this feedback remains unclear [13]. Here we reveal that polarization of the auxin efflux carrier PIN-FORMED 1 (PIN1) is regulated by the auxin response transcription factor MONOPTEROS (MP) [14]. We find that in the shoot, cell polarity patterns follow MP expression, which in turn follows auxin distribution patterns. By perturbing MP activity both globally and locally, we show that localized MP activity is necessary for the generation of polarity convergence patterns and that localized MP expression is sufficient to instruct PIN1 polarity directions non-cell autonomously, toward MP-expressing cells. By expressing MP in the epidermis of mp mutants, we further show that although MP activity in a single-cell layer is sufficient to promote polarity convergence patterns, MP in sub-epidermal tissues helps anchor these polarity patterns to the underlying cells. Overall, our findings reveal a patterning module in plants that determines organ position by orienting transport of the hormone auxin toward cells with high levels of MP-mediated auxin signaling. We propose that this feedback process acts broadly to generate periodic plant architectures.

  1. Coordination and decision making of regulation, operation, and market activities in power systems

    NASA Astrophysics Data System (ADS)

    Nakashima, Tomoaki

    Electric power has been traditionally supplied to customers at regulated rates by vertically integrated utilities (VIUs), which own generation, transmission, and distribution systems. However, the regulatory authorities of VIUs are promoting competition in their businesses to lower the price of electric energy. Consequently, in new deregulated circumstances, many suppliers and marketers compete in the generation market, and conflict of interest may often occur over transmission. Therefore, a neutral entity, called an independent system operator (ISO), which operates the power system independently, has been established to give market participants nondiscriminatory access to transmission sectors with a natural monopoly, and to facilitate competition in generation sectors. Several types of ISOs are established at present, with their respective regions and authorities. The ISO receives many requests from market participants to transfer power, and must evaluate the feasibility of their requests under the system's condition. In the near future, regulatory authorities may impose various objectives on the ISOs. Then, based on the regulators' policies, the ISO must determine the optimal schedules from feasible solutions, or change the market participants' requests. In a newly developed power market, market participants will conduct their transactions in order to maximize their profit. The most crucial information in conducting power transactions is price and demand. A direct transaction between suppliers and consumers may become attractive because of its stability of price, while in a power exchange market, gaming and speculation of participants may push up electricity prices considerably. To assist the consumers in making effective decisions, suitable methods for forecasting volatile market price are necessary. This research has been approached from three viewpoints: Firstly, from the system operator's point of view, desirable system operation and power market structure

  2. Environmental laws regulating chemicals: Uses of information in decision making under environmental statutes

    SciTech Connect

    Gaba, J.M.

    1990-12-31

    Three areas are addressed in this paper: generic issues that arise simply in the process of decision-making under environmental statutes; different decision-making standards under various environmental statutes; and efforts to legislate a {open_quotes}safe{close_quotes} or {open_quotes}acceptable{close_quotes} risk from exposure to carcinogenic chemicals.

  3. Dicer-1-dependent Dacapo suppression acts downstream of Insulin receptor in regulating cell division of Drosophila germline stem cells

    PubMed Central

    Yu, Jenn-Yah; Reynolds, Steven H.; Hatfield, Steve D.; Shcherbata, Halyna R.; Fischer, Karin A.; Ward, Ellen J.; Long, Dang; Ding, Ye; Ruohola-Baker, Hannele

    2009-01-01

    Summary It is important to understand the regulation of stem cell division because defects in this process can cause altered tissue homeostasis or cancer. The cyclin-dependent kinase inhibitor Dacapo (Dap), a p21/p27 homolog, acts downstream of the microRNA (miRNA) pathway to regulate the cell cycle in Drosophila melanogaster germline stem cells (GSCs). Tissue-extrinsic signals, including insulin, also regulate cell division of GSCs. We report that intrinsic and extrinsic regulators intersect in GSC division control; the Insulin receptor (InR) pathway regulates Dap levels through miRNAs, thereby controlling GSC division. Using GFP-dap 3′UTR sensors in vivo, we show that in GSCs the dap 3′UTR is responsive to Dicer-1, an RNA endonuclease III required for miRNA processing. Furthermore, the dap 3′UTR can be directly targeted by miR-7, miR-278 and miR-309 in luciferase assays. Consistent with this, miR-278 and miR-7 mutant GSCs are partially defective in GSC division and show abnormal cell cycle marker expression, respectively. These data suggest that the GSC cell cycle is regulated via the dap 3′UTR by multiple miRNAs. Furthermore, the GFP-dap 3′UTR sensors respond to InR but not to TGF-β signaling, suggesting that InR signaling utilizes Dap for GSC cell cycle regulation. We further demonstrate that the miRNA-based Dap regulation may act downstream of InR signaling; Dcr-1 and Dap are required for nutrition-dependent cell cycle regulation in GSCs and reduction of dap partially rescues the cell cycle defect of InR-deficient GSCs. These data suggest that miRNA- and Dap-based cell cycle regulation in GSCs can be controlled by InR signaling. PMID:19336466

  4. Dicer-1-dependent Dacapo suppression acts downstream of Insulin receptor in regulating cell division of Drosophila germline stem cells.

    PubMed

    Yu, Jenn-Yah; Reynolds, Steven H; Hatfield, Steve D; Shcherbata, Halyna R; Fischer, Karin A; Ward, Ellen J; Long, Dang; Ding, Ye; Ruohola-Baker, Hannele

    2009-05-01

    It is important to understand the regulation of stem cell division because defects in this process can cause altered tissue homeostasis or cancer. The cyclin-dependent kinase inhibitor Dacapo (Dap), a p21/p27 homolog, acts downstream of the microRNA (miRNA) pathway to regulate the cell cycle in Drosophila melanogaster germline stem cells (GSCs). Tissue-extrinsic signals, including insulin, also regulate cell division of GSCs. We report that intrinsic and extrinsic regulators intersect in GSC division control; the Insulin receptor (InR) pathway regulates Dap levels through miRNAs, thereby controlling GSC division. Using GFP-dap 3'UTR sensors in vivo, we show that in GSCs the dap 3'UTR is responsive to Dicer-1, an RNA endonuclease III required for miRNA processing. Furthermore, the dap 3'UTR can be directly targeted by miR-7, miR-278 and miR-309 in luciferase assays. Consistent with this, miR-278 and miR-7 mutant GSCs are partially defective in GSC division and show abnormal cell cycle marker expression, respectively. These data suggest that the GSC cell cycle is regulated via the dap 3'UTR by multiple miRNAs. Furthermore, the GFP-dap 3'UTR sensors respond to InR but not to TGF-beta signaling, suggesting that InR signaling utilizes Dap for GSC cell cycle regulation. We further demonstrate that the miRNA-based Dap regulation may act downstream of InR signaling; Dcr-1 and Dap are required for nutrition-dependent cell cycle regulation in GSCs and reduction of dap partially rescues the cell cycle defect of InR-deficient GSCs. These data suggest that miRNA- and Dap-based cell cycle regulation in GSCs can be controlled by InR signaling.

  5. Notification: Preliminary Research on EPA's Decision Making Process to Release Information Under the Freedom of Information Act

    EPA Pesticide Factsheets

    July 19, 2013. The Office of Inspector General plans to begin preliminary research on the U.S. Environmental Protection Agency’s process for deciding to release information requested under the Freedom of Information Act.

  6. WORKING AND CARING: THE SIMULTANEOUS DECISION OF LABOR FORCE PARTICIPATION AND INFORMAL ELDERLY AND CHILD SUPPORT ACT IVITIES IN MEXICO.

    PubMed

    van Gameren, Edwin; Velandia Naranjo, Durfari

    2015-11-02

    We analyze factors determining women's decisions to participate in the labor market and provide elderly care and nonfinancial support to their (grand)children. We use data from the Mexican Health and Aging Study, a survey of people aged 50 and over, applying a three-equation, reduced-form SUR model. Results suggest that care needs are the driving force behind caregiving activities. Traditional roles also appear to be relevant in the labor force participation decision: women with a closer labor market connection when they were young are more likely to work. Simulations of demographic changes illustrate potential effects for future caregiving and participation rates.

  7. WORKING AND CARING: THE SIMULTANEOUS DECISION OF LABOR FORCE PARTICIPATION AND INFORMAL ELDERLY AND CHILD SUPPORT ACT IVITIES IN MEXICO*

    PubMed Central

    van Gameren, Edwin; Velandia Naranjo, Durfari

    2016-01-01

    We analyze factors determining women’s decisions to participate in the labor market and provide elderly care and nonfinancial support to their (grand)children. We use data from the Mexican Health and Aging Study, a survey of people aged 50 and over, applying a three-equation, reduced-form SUR model. Results suggest that care needs are the driving force behind caregiving activities. Traditional roles also appear to be relevant in the labor force participation decision: women with a closer labor market connection when they were young are more likely to work. Simulations of demographic changes illustrate potential effects for future caregiving and participation rates. PMID:26924883

  8. 75 FR 63382 - Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations; Defining...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... 31 CFR Part 103 RIN 1506-AA93 Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act... Crimes Enforcement Network (``FinCEN''), Treasury. ACTION: Final rule; extension of compliance date... to recordkeeping requirements under the Securities Exchange Act of 1934.\\8\\ In light of...

  9. 77 FR 54843 - Integrated Mortgage Disclosures Under the Real Estate Settlement Procedure Act (Regulation X) and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... PROTECTION 12 CFR Part 1026 RIN 3170-AA19 Integrated Mortgage Disclosures Under the Real Estate Settlement... certain disclosure requirements of the Truth in Lending Act (TILA) and the Real Estate Settlement... disclosure requirements of the Truth in Lending Act (TILA) and the Real Estate Settlement Procedures...

  10. 76 FR 40367 - Federal Acquisition Regulation; Information Collection; Buy American Act-Construction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-08

    ...-9, Buy American Act--Construction Materials, and FAR 52.225-11, Buy American Act--Construction Materials under Trade Agreements, provide that offerors/contractors requesting to use foreign construction material, other than construction material eligible under a trade agreement, shall provide...

  11. 77 FR 43782 - Acquisition Regulations; Buy Indian Act; Procedures for Contracting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-26

    ... Planning and Review (Executive Order 12866) B. Regulatory Flexibility Act C. Small Business Regulatory... there was an inconsistency in the proposed rule regarding small business set-asides for acquisitions... Small Business Act Requirements, as it governs small purchases, and at the same time continue its...

  12. RECOVERY ACT - Methods for Decision under Technological Change Uncertainty and Risk Assessment for Integrated Assessment of Climate Change

    SciTech Connect

    Webster, Mort D.

    2015-11-30

    This report presents the final outcomes and products of the project as performed both at the Massachusetts Institute of Technology and subsequently at Pennsylvania State University. The research project can be divided into three main components: methodology development for decision-making under uncertainty, improving the resolution of the electricity sector to improve integrated assessment, and application of these methods to integrated assessment.

  13. RECOVERY ACT - Methods for Decision under Technological Change Uncertainty and Risk Assessment for Integrated Assessment of Climate Change

    SciTech Connect

    Webster, Mort David

    2015-03-10

    This report presents the final outcomes and products of the project as performed at the Massachusetts Institute of Technology. The research project consists of three main components: methodology development for decision-making under uncertainty, improving the resolution of the electricity sector to improve integrated assessment, and application of these methods to integrated assessment. Results in each area is described in the report.

  14. MicroRNAs act complementarily to regulate disease-related mRNA modules in human diseases.

    PubMed

    Chavali, Sreenivas; Bruhn, Sören; Tiemann, Katrin; Saetrom, Pål; Barrenäs, Fredrik; Saito, Takaya; Kanduri, Kartiek; Wang, Hui; Benson, Mikael

    2013-11-01

    MicroRNAs (miRNAs) play a key role in regulating mRNA expression, and individual miRNAs have been proposed as diagnostic and therapeutic candidates. The identification of such candidates is complicated by the involvement of multiple miRNAs and mRNAs as well as unknown disease topology of the miRNAs. Here, we investigated if disease-associated miRNAs regulate modules of disease-associated mRNAs, if those miRNAs act complementarily or synergistically, and if single or combinations of miRNAs can be targeted to alter module functions. We first analyzed publicly available miRNA and mRNA expression data for five different diseases. Integrated target prediction and network-based analysis showed that the miRNAs regulated modules of disease-relevant genes. Most of the miRNAs acted complementarily to regulate multiple mRNAs. To functionally test these findings, we repeated the analysis using our own miRNA and mRNA expression data from CD4+ T cells from patients with seasonal allergic rhinitis. This is a good model of complex diseases because of its well-defined phenotype and pathogenesis. Combined computational and functional studies confirmed that miRNAs mainly acted complementarily and that a combination of two complementary miRNAs, miR-223 and miR-139-3p, could be targeted to alter disease-relevant module functions, namely, the release of type 2 helper T-cell (Th2) cytokines. Taken together, our findings indicate that miRNAs act complementarily to regulate modules of disease-related mRNAs and can be targeted to alter disease-relevant functions.

  15. Sexually dimorphic control of gene expression in sensory neurons regulates decision-making behavior in C. elegans.

    PubMed

    Hilbert, Zoë A; Kim, Dennis H

    2017-01-24

    Animal behavior is directed by the integration of sensory information from internal states and the environment. Neuroendocrine regulation of diverse behaviors of Caenorhabditis elegans is under the control of the DAF-7/TGF-β ligand that is secreted from sensory neurons. Here, we show that C. elegans males exhibit an altered, male-specific expression pattern of daf-7 in the ASJ sensory neuron pair with the onset of reproductive maturity, which functions to promote male-specific mate-searching behavior. Molecular genetic analysis of the switch-like regulation of daf-7 expression in the ASJ neuron pair reveals a hierarchy of regulation among multiple inputs-sex, age, nutritional status, and microbial environment-which function in the modulation of behavior. Our results suggest that regulation of gene expression in sensory neurons can function in the integration of a wide array of sensory information and facilitate decision-making behaviors in C. elegans.

  16. 75 FR 1408 - Notice of Availability of the Record of Decision for the Lincoln County Land Act Groundwater...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-11

    ... Lincoln County Water District (LCWD) a ROW grant for the construction, operation, maintenance and termination of the mainline water pipeline, main power lines, pump stations, regulating tanks and...

  17. 28 CFR Appendix C to Subpart G of... - Department Regulations Under Title VI of the Civil Rights Act of 1964 (28 CFR 42.106-42.110...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the Civil Rights Act of 1964 (28 CFR 42.106-42.110) Which Apply to This Subpart C Appendix C to... Activities-Implementation of Section 504 of the Rehabilitation Act of 1973 Pt. 42, Subpt. G, App. C Appendix C to Subpart G of Part 42—Department Regulations Under Title VI of the Civil Rights Act of 1964...

  18. 28 CFR Appendix C to Subpart G of... - Department Regulations Under Title VI of the Civil Rights Act of 1964 (28 CFR 42.106-42.110...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the Civil Rights Act of 1964 (28 CFR 42.106-42.110) Which Apply to This Subpart C Appendix C to... Activities-Implementation of Section 504 of the Rehabilitation Act of 1973 Pt. 42, Subpt. G, App. C Appendix C to Subpart G of Part 42—Department Regulations Under Title VI of the Civil Rights Act of 1964...

  19. 28 CFR Appendix C to Subpart G of... - Department Regulations Under Title VI of the Civil Rights Act of 1964 (28 CFR 42.106-42.110...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the Civil Rights Act of 1964 (28 CFR 42.106-42.110) Which Apply to This Subpart C Appendix C to... Activities-Implementation of Section 504 of the Rehabilitation Act of 1973 Pt. 42, Subpt. G, App. C Appendix C to Subpart G of Part 42—Department Regulations Under Title VI of the Civil Rights Act of 1964...

  20. 28 CFR Appendix C to Subpart G of... - Department Regulations Under Title VI of the Civil Rights Act of 1964 (28 CFR 42.106-42.110...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the Civil Rights Act of 1964 (28 CFR 42.106-42.110) Which Apply to This Subpart C Appendix C to... Activities-Implementation of Section 504 of the Rehabilitation Act of 1973 Pt. 42, Subpt. G, App. C Appendix C to Subpart G of Part 42—Department Regulations Under Title VI of the Civil Rights Act of 1964...

  1. 28 CFR Appendix C to Subpart G of... - Department Regulations Under Title VI of the Civil Rights Act of 1964 (28 CFR 42.106-42.110...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the Civil Rights Act of 1964 (28 CFR 42.106-42.110) Which Apply to This Subpart C Appendix C to... Activities-Implementation of Section 504 of the Rehabilitation Act of 1973 Pt. 42, Subpt. G, App. C Appendix C to Subpart G of Part 42—Department Regulations Under Title VI of the Civil Rights Act of 1964...

  2. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? These regulations define the nature and Indian...

  3. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? These regulations define the nature and Indian...

  4. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? These regulations define the nature and Indian...

  5. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? These regulations define the nature and Indian...

  6. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? These regulations define the nature and Indian...

  7. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and Fair... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L....

  8. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and Fair... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L....

  9. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and Fair... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L....

  10. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and Fair... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L....

  11. GP Supervisors' Experience in Supporting Self-Regulated Learning: A Balancing Act

    ERIC Educational Resources Information Center

    Sagasser, Margaretha H.; Kramer, Anneke W. M.; van Weel, Chris; van der Vleuten, Cees P. M.

    2015-01-01

    Self-regulated learning is essential for professional development and lifelong learning. As self-regulated learning has many inaccuracies, the need to support self-regulated learning has been recommended. Supervisors can provide such support. In a prior study trainees reported on the variation in received supervisor support. This study aims at…

  12. Reflections on Decisions Made on the Well-Established Use of Medicinal Products by EU Regulators and the ECJ

    PubMed Central

    Borg, John Joseph; Laslop, Andrea; Pani, Luca; Maciulaitis, Romaldas; Melchiorri, Daniela

    2014-01-01

    Abstract Background: In the European Union (EU), a medicinal product needs a marketing authorization (MA) to be placed on the market. The EU’s medicinal products’ legislative framework allows for a reduced application for medicines outside their data exclusivity. One such type of application is the well-established use (WEU) medicinal product application (i.e. bibliographic applications). Recently, these MA applications have been subject to arbitration procedures at the European Medicines Agency’s Committee for Medicinal Products for Human Use (CHMP) because of disagreements between member states during the authorisation process. This paper reflects on these cases and highlights their potential impact on future WEU applications. Methods: Decisions adopted by the European Commission on WEU applications between 2009 and 2012 were identified from the EU Community Register on medicinal products for human use. Subsequently, decisions were reviewed to understand the potential serious risk to public health (PSRPH) that EU regulators raised during MA application procedures. Results: Four decisions were adopted by the EU commission between 2009 and 2012. Three followed disagreements between member states on PSRPH grounds. One decision was the outcome of a centralised marketing authorisation application. Six key messages were identified from the four cases reviewed and presented. Conclusion: A guideline on WEU to implement the technical specifications to fulfil Annex I of Directive 2001/83/EC for MA applications is not available. Thus, reflections on recent decisions on WEU applications provide scientific direction to the industry as well as the medicinal product regulators on the documentation required to successfully file and obtain a WEU MA. PMID:25853067

  13. RTI v medical ethics: some questions arising from the recent decision of the Chief Information Commissioner under the RTI Act.

    PubMed

    Nair, M R Hariharan

    2015-01-01

    Medical ethics attaches the utmost priority to the confidentiality of medical records. Hence, the decision of the Chief Information Commissioner (CIC) rendered on April 10, 2015 in Case No: CIC/KY/A/2014/001348SA Ms Jyoti Jeena v. PIO, Institute of Human Behaviour & Allied Science (hereinafter referred to as Jyoti Jeena), that the wife-applicant is entitled to get copies of the medical records of her estranged husband has raised many eyebrows.

  14. Drinking water regulations under the Safe Drinking Water Act. Fact sheet

    SciTech Connect

    Not Available

    1990-12-01

    The fact sheet describes the requirements covered under the 1986 amendments to the Safe Drinking Water Act. Levels of various contaminants (including radio nuclides) are explained. Also discussed are the Surface Water Treatment Rule and the Total Coliforms Rule.

  15. The Real Education for Healthy Youth Act: Honest, Age Appropriate Sexual Health Education for Responsible Decision Making. Policy Brief

    ERIC Educational Resources Information Center

    Phillip, Jendayi

    2012-01-01

    The Real Education for Healthy Youth Act (S. 1782/H.R. 3324), introduced in November 2011 by Senator Frank Lautenberg (D-NJ) and Representative Barbara Lee (D-CA), would ensure that federal funding is allocated to comprehensive sexual health education programs that provide young people with the skills and information they need to make informed,…

  16. The second act. With Obama and the reform law here to stay, big decisions are ahead for healthcare.

    PubMed

    Daly, Rich; Zigmond, Jessica

    2012-11-12

    The re-election of President Barack Obama to a second term and the preservation of a Democratic majority in the Senate removed doubts about the survival of the Patient Protection and Affordable Care Act as the law of the land.

  17. Regulation of caulimovirus gene expression and the involvement of cis-acting elements on both viral transcripts.

    PubMed

    Scholthof, H B; Wu, F C; Gowda, S; Shepherd, R J

    1992-09-01

    In a further analysis of gene regulation of figwort mosaic virus (FMV), a caulimovirus, we studied transient gene expression with modified viral genomes in Nicotiana edwardsonii cell suspension protoplasts. The results demonstrated that the presence of the promoter for the full-length RNA interferes with expression from the separate downstream promoter for gene VI. In addition, expression of gene VI was inhibited by cis-acting sequences within gene VI itself. Both inhibitory effects could be partially relieved by coelectroporation with a plasmid that produces gene VI protein, demonstrating that expression of gene VI is transactivated by its own product. Subsequent expression studies with partially redundant FMV plasmids containing a reporter gene in frame with gene IV showed that efficient transactivation of CAT expression relies on a cis-acting element inside the downstream gene VI. Insertions of a transcriptional terminator upstream of the cis-acting element for premature termination of transcription showed that the cis-acting region is not a DNA element but is active only as a feature of the RNA transcript. We conclude that the cis-acting element, together with the transacting gene VI product, enhances expression of all major genes, including gene VI, from the polycistronic mRNA and the separate mRNA for gene VI.

  18. Neuronal correlates of decisions to speak and act: Spontaneous emergence and dynamic topographies in a computational model of frontal and temporal areas

    PubMed Central

    Garagnani, Max; Pulvermüller, Friedemann

    2013-01-01

    The neural mechanisms underlying the spontaneous, stimulus-independent emergence of intentions and decisions to act are poorly understood. Using a neurobiologically realistic model of frontal and temporal areas of the brain, we simulated the learning of perception–action circuits for speech and hand-related actions and subsequently observed their spontaneous behaviour. Noise-driven accumulation of reverberant activity in these circuits leads to their spontaneous ignition and partial-to-full activation, which we interpret, respectively, as model correlates of action intention emergence and action decision-and-execution. Importantly, activity emerged first in higher-association prefrontal and temporal cortices, subsequently spreading to secondary and finally primary sensorimotor model-areas, hence reproducing the dynamics of cortical correlates of voluntary action revealed by readiness-potential and verb-generation experiments. This model for the first time explains the cortical origins and topography of endogenous action decisions, and the natural emergence of functional specialisation in the cortex, as mechanistic consequences of neurobiological principles, anatomical structure and sensorimotor experience. PMID:23489583

  19. Behavioral contagion during learning about another agent’s risk-preferences acts on the neural representation of decision-risk

    PubMed Central

    Suzuki, Shinsuke; Jensen, Emily L. S.; Bossaerts, Peter; O’Doherty, John P.

    2016-01-01

    Our attitude toward risk plays a crucial role in influencing our everyday decision-making. Despite its importance, little is known about how human risk-preference can be modulated by observing risky behavior in other agents at either the behavioral or the neural level. Using fMRI combined with computational modeling of behavioral data, we show that human risk-preference can be systematically altered by the act of observing and learning from others’ risk-related decisions. The contagion is driven specifically by brain regions involved in the assessment of risk: the behavioral shift is implemented via a neural representation of risk in the caudate nucleus, whereas the representations of other decision-related variables such as expected value are not affected. Furthermore, we uncover neural computations underlying learning about others’ risk-preferences and describe how these signals interact with the neural representation of risk in the caudate. Updating of the belief about others’ preferences is associated with neural activity in the dorsolateral prefrontal cortex (dlPFC). Functional coupling between the dlPFC and the caudate correlates with the degree of susceptibility to the contagion effect, suggesting that a frontal–subcortical loop, the so-called dorsolateral prefrontal–striatal circuit, underlies the modulation of risk-preference. Taken together, these findings provide a mechanistic account for how observation of others’ risky behavior can modulate an individual’s own risk-preference. PMID:27001826

  20. Vhnf1 acts downstream of Bmp, Fgf, and RA signals to regulate endocrine beta cell development in zebrafish.

    PubMed

    Song, Jianbo; Kim, Hyon J; Gong, Zhiyuan; Liu, Ning-Ai; Lin, Shuo

    2007-03-15

    Bmp, Fgf, and retinoic acid (RA) signals have been implicated as regulators of pancreas development. However, the integration of these signaling pathways in vivo is not fully understood. Variant hnf1 (Vhnf1) is a transcription factor involved in pancreas, liver, and kidney development and its mutation in zebrafish causes underdeveloped pancreas and liver. We investigated the signaling pathways that regulate vhnf1 expression during pancreas development. First, we showed that Bmp activity is required for vhnf1 expression in the endoderm. In chordin (a Bmp antagonist) morpholino (MO)-injected embryos, vhnf1 expression in endoderm and in endocrine beta cells is expanded. On the other hand, in alk8 (a type I TGFbeta receptor) MO-injected embryos, vhnf1 expression in the endoderm is significantly reduced. Second, we showed that Fgf signaling participates in regulation of pancreas development through the vhnf1 pathway. Third, we demonstrated that RA fails to rescue reduction of insulin expression in vhnf1 mutants, whereas overexpression of vhnf1 restores insulin expression that is repressed by treatment with a RA receptor inhibitor. And finally, we revealed that both Bmp and Fgf signals act genetically upstream of RA in directing pancreas development. Taken together, our data establish that vhnf1 acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish.

  1. The implications of RCRA (Resource Conservation and Recovery Act) regulation for the disposal of transuranic and high-level waste

    SciTech Connect

    Sigmon, C.F.; Sharples, F.E.; Smith, E.D.

    1988-01-01

    In May of 1987 the Department of Energy (DOE) published a rule interpreting the definition of ''byproduct'' under the Atomic Energy Act. This byproduct rule clarified the role of the Resource Conservation and Recovery Act (RCRA) in the regulation of DOE's radioactive waste management activities. According to the rule, only the radioactive portion of DOE's mixed radioactive and hazardous waste (mixed waste), including mixed transuranic (TRU) and high-level waste (HLW), is exempt from RCRA under the byproduct exemption. The portion of a waste that is hazardous as defined by RCRA is subject to full regulation under RCRA. Because the radioactive and hazardous portions of m any, if not most, DOE wastes are likely to be inseparable, the rule in effect makes most mixed wastes subject to dual regulation. The potential application of RCRA to facilities such as the Waste Isolation Pilot Plant (WIPP) and the HLW repository creates unique challenges for both the DOE and regulatory authorities. Strategies must be developed to assure compliance with RCRA without either causing excessive administrative burdens or abandoning the goal of minimizing radiation exposure. This paper will explore some of the potential regulatory options for and recent trends in the regulation of TRU and HLW under RCRA.

  2. Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination

    PubMed Central

    Miyamoto, Yuki; Yamamori, Natsuki; Torii, Tomohiro; Tanoue, Akito; Yamauchi, Junji

    2014-01-01

    Oligodendrocyte precursor cells differentiate to produce myelin sheaths that insulate axons to ensure fast propagation of action potentials. Many aspects of differentiation are regulated by multiple extracellular signals. However, their intracellular signalings remain elusive. We show that Rab35 and its effector, ACAP2, a GTPase-activating protein that switches off Arf6 activity, negatively regulate oligodendrocyte morphological differentiation. Knockdown of Rab35 or ACAP2 with their respective small interfering RNAs promotes differentiation. As differentiation initiates, the activities of Rab35 and ACAP2 are down-regulated. The activity of Arf6, in contrast, is up-regulated. Arf6 knockdown inhibits differentiation, indicating that Rab35 and ACAP2 negatively regulate differentiation by down-regulating Arf6. Importantly, as differentiation proceeds, the activity of cytohesin-2, a guanine nucleotide exchange factor that switches on Arf6 activity, is up-regulated. Pharmacological inhibition of cytohesin-2 inhibits differentiation, suggesting that cytohesin-2 promotes differentiation by activating Arf6. Furthermore, using oligodendrocyte-neuronal cocultures, we find that knockdown of Rab35 or ACAP2 promotes myelination, whereas inhibition of cytohesin-2 or knockdown of Arf6 inhibits myelination. Thus Rab35/ACAP2 and cytohesin-2 antagonistically control oligodendrocyte differentiation and myelination through Arf6 regulation, presenting a unique small GTPase on/off switching mechanism. PMID:24600047

  3. 75 FR 46859 - Lacey Act Implementation Plan; Definitions for Exempt and Regulated Articles

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-04

    ... Implementation Plan; Definitions for Exempt and Regulated Articles AGENCY: Animal and Plant Health Inspection... proposing to establish definitions for the terms ``common cultivar'' and ``common food crop.'' The... regulation. Our proposed definitions would specify which plants and plant products will be subject to...

  4. 78 FR 40940 - Lacey Act Implementation Plan; Definitions for Exempt and Regulated Articles

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-09

    ...; Definitions for Exempt and Regulated Articles AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION... definitions for the terms ``common cultivar'' and ``common food crop'' and several related terms. The... regulation. Our definitions specify which plants and plant products will be excluded from the provisions...

  5. 76 FR 10527 - Regulatory Flexibility Act: Section 610 Review of National Organic Program Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-25

    ... Organic Program Regulations AGENCY: Agricultural Marketing Service, USDA. ACTION: Review and request for... National Organic Program (NOP) regulations (7 CFR part 205). This review will be conducted under criteria... Specialist, National Organic Program, USDA-AMS-NOP, 1400 Independence Ave., SW., Room 2646-So., Ag Stop...

  6. Fnr (EtrA) acts as a fine-tuning regulator of anaerobic metabolism in Shewanella oneidensis MR-1

    SciTech Connect

    Cruz-Garza, Claribel; Murray, Alison E.; Rodrigues, Jorge L.M.; Gralnick, Jeffrey A.; McCue, Lee Ann; Romine, Margaret F.; Loffler, F. E.; Tiedje, James M.

    2011-03-30

    EtrA in Shewanella oneidensis MR-1, a model organism for study of adaptation to varied redox niches, shares 73.6% and 50.8% amino acid sequence identity with the oxygen-sensing regulators Fnr in E. coli and Anr in Pseudomonas aeruginosa, respectively; however, its regulatory role of anaerobic metabolism in Shewanella spp. is not well understood. The expression of the nap genes, nrfA, cymA and hcp was significantly reduced in etrA deletion mutant EtrA7-1; however, limited anaerobic growth and nitrate reduction occurred, suggesting that multiple regulators control nitrate reduction in this strain. Dimethyl sulfoxide (DMSO) and fumarate reductase gene expression was down regulated at least 2-fold and the EtrA7-1 mutant grew poorly with fumarate and dimethyl sulfoxide (DMSO), suggesting both respiratory pathways are under EtrA control. Transcript analysis further suggested a role of EtrA in prophage activation and down regulation of genes implicated in aerobic metabolism. In contrast to previous studies that attributed a minor regulatory role to EtrA in Shewanella spp., this study demonstrates that EtrA acts as a global transcriptional regulator and confers physiological advantages to strain MR-1 under certain growth conditions. In conjunction with other regulators, EtrA fine-tunes the expression of genes involved in anaerobic metabolism in S. oneidensis strain MR-1.

  7. Toward an operational model of decision making, emotional regulation, and mental health impact.

    PubMed

    Collura, Thomas Francis; Zalaquett, Ronald P; Bonnstetter, Carlos Joyce; Chatters, Seria J

    2014-01-01

    Current brain research increasingly reveals the underlying mechanisms and processes of human behavior, cognition, and emotion. In addition to being of interest to a wide range of scientists, educators, and professionals, as well as laypeople, brain-based models are of particular value in a clinical setting. Psychiatrists, psychologists, counselors, and other mental health professionals are in need of operational models that integrate recent findings in the physical, cognitive, and emotional domains, and offer a common language for interdisciplinary understanding and communication. Based on individual traits, predispositions, and responses to stimuli, we can begin to identify emotional and behavioral pathways and mental processing patterns. The purpose of this article is to present a brain-path activation model to understand individual differences in decision making and psychopathology. The first section discusses the role of frontal lobe electroencephalography (EEG) asymmetry, summarizes state- and trait-based models of decision making, and provides a more complex analysis that supplements the traditional simple left-right brain model. Key components of the new model are the introduction of right hemisphere parallel and left hemisphere serial scanning in rendering decisions, and the proposition of pathways that incorporate both past experiences as well as future implications into the decision process. Main attributes of each decision-making mechanism are provided. The second section applies the model within the realm of clinical mental health as a tool to understand specific human behavior and pathology. Applications include general and chronic anxiety, depression, paranoia, risk taking, and the pathways employed when well-functioning operational integration is observed. Finally, specific applications such as meditation and mindfulness are offered to facilitate positive functioning.

  8. 78 FR 60686 - Regulations Implementing the Byrd Amendments to the Black Lung Benefits Act: Determining Coal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    ... document corrects that error and changes the contact information. DATES: Effective October 25, 2013. FOR FURTHER INFORMATION CONTACT: Carol A. Campbell, Acting Deputy Director, Division of Coal Mine Workers... the FOR FURTHER INFORMATION CONTACT section has changed as set forth above. 2. On page 59112, in...

  9. 78 FR 27078 - Native American Graves Protection and Repatriation Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... through a commitment to consultation with Indian Tribes and recognition of their right to self-governance.... National Environmental Policy Act This rule does not constitute a major Federal action significantly affecting the quality of the human environment. A detailed statement under the National Environmental...

  10. 77 FR 74546 - Posting of Pamphlet Provided for in the International Marriage Broker Regulation Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-14

    ... Act, Title D of Public Law 109-162, provided that the Secretary of Homeland Security, in consultation... such pamphlet be posted on the Web sites of the Department of State and all consular posts processing... posted on the Web site of the Bureau of Consular Affairs of the Department of State at:...

  11. Rules and Regulations for Implementing the Pennsylvania Nonpublic Elementary and Secondary Education Act.

    ERIC Educational Resources Information Center

    Pennsylvania State Dept. of Public Instruction, Harrisburg. Office for Aid to Nonpublic Education.

    The Pennsylvania Nonpublic Elementary and Secondary Education Act was passed into law in June 1968. It authorizes the Superintendent of Public Instruction of that State to enter into contracts to purchase secular educational service from nonpublic schools in Pennsylvania. This guide contains (1) the law, (2) definitions of terms, (3) a statement…

  12. Pneumococcal galactose catabolism is controlled by multiple regulators acting on pyruvate formate lyase

    PubMed Central

    Al-Bayati, Firas A. Y.; Kahya, Hasan F. H.; Damianou, Andreas; Shafeeq, Sulman; Kuipers, Oscar P.; Andrew, Peter W.; Yesilkaya, Hasan

    2017-01-01

    Catabolism of galactose by Streptococcus pneumoniae alters the microbe’s metabolism from homolactic to mixed acid fermentation, and this shift is linked to the microbe’s virulence. However, the genetic basis of this switch is unknown. Pyruvate formate lyase (PFL) is a crucial enzyme for mixed acid fermentation. Functional PFL requires the activities of two enzymes: pyruvate formate lyase activating enzyme (coded by pflA) and pyruvate formate lyase (coded by pflB). To understand the genetic basis of mixed acid fermentation, transcriptional regulation of pflA and pflB was studied. By microarray analysis of ΔpflB, differential regulation of several transcriptional regulators were identified, and CcpA, and GlnR’s role in active PFL synthesis was studied in detail as these regulators directly interact with the putative promoters of both pflA and pflB, their mutation attenuated pneumococcal growth, and their expression was induced on host-derived sugars, indicating that these regulators have a role in sugar metabolism, and multiple regulators are involved in active PFL synthesis. We also found that the influence of each regulator on pflA and pflB expression was distinct in terms of activation and repression, and environmental condition. These results show that active PFL synthesis is finely tuned, and feed-back inhibition and activation are involved. PMID:28240278

  13. Identification of cis-acting elements and splicing factors involved in the regulation of BIM Pre-mRNA splicing.

    PubMed

    Juan, Wen Chun; Roca, Xavier; Ong, S Tiong

    2014-01-01

    Aberrant changes in the expression of the pro-apoptotic protein, BCL-2-like 11 (BIM), can result in either impaired or excessive apoptosis, which can contribute to tumorigenesis and degenerative disorders, respectively. Altering BIM pre-mRNA splicing is an attractive approach to modulate apoptosis because BIM activity is partly determined by the alternative splicing of exons 3 or 4, whereby exon 3-containing transcripts are not apoptotic. Here we identified several cis-acting elements and splicing factors involved in BIM alternative splicing, as a step to better understand the regulation of BIM expression. We analyzed a recently discovered 2,903-bp deletion polymorphism within BIM intron 2 that biased splicing towards exon 3, and which also impaired BIM-dependent apoptosis. We found that this region harbors multiple redundant cis-acting elements that repress exon 3 inclusion. Furthermore, we have isolated a 23-nt intronic splicing silencer at the 3' end of the deletion that is important for excluding exon 3. We also show that PTBP1 and hnRNP C repress exon 3 inclusion, and that downregulation of PTBP1 inhibited BIM-mediated apoptosis. Collectively, these findings start building our understanding of the cis-acting elements and splicing factors that regulate BIM alternative splicing, and also suggest potential approaches to alter BIM splicing for therapeutic purposes.

  14. Market effects of environmental regulation: coal, railroads, and the 1990 Clean Air Act

    SciTech Connect

    Busse, M.R.; Keohane, N.O.

    2007-01-01

    Many environmental regulations encourage the use of 'clean' inputs. When the suppliers of such an input have market power, environmental regulation will affect not only the quantity of the input used but also its price. We investigate the effect of the Title IV emissions trading program for sulfur dioxide on the market for low-sulfur coal. We find that the two railroads transporting coal were able to price discriminate on the basis of environmental regulation and geographic location. Delivered prices rose for plants in the trading program relative to other plants, and by more at plants near a low-sulfur coal source.

  15. 43 CFR 10010.47 - Pre-decision referrals to CEQ.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... POLICY ACT Relationship to Decision-Making § 10010.47 Pre-decision referrals to CEQ. (a) Upon receipt of... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Pre-decision referrals to CEQ. 10010.47 Section 10010.47 Public Lands: Interior Regulations Relating to Public Lands (Continued) UTAH...

  16. 43 CFR 10010.47 - Pre-decision referrals to CEQ.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... POLICY ACT Relationship to Decision-Making § 10010.47 Pre-decision referrals to CEQ. (a) Upon receipt of... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Pre-decision referrals to CEQ. 10010.47 Section 10010.47 Public Lands: Interior Regulations Relating to Public Lands (Continued) UTAH...

  17. 43 CFR 10010.47 - Pre-decision referrals to CEQ.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... POLICY ACT Relationship to Decision-Making § 10010.47 Pre-decision referrals to CEQ. (a) Upon receipt of... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Pre-decision referrals to CEQ. 10010.47 Section 10010.47 Public Lands: Interior Regulations Relating to Public Lands (Continued) UTAH...

  18. 43 CFR 10010.47 - Pre-decision referrals to CEQ.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... POLICY ACT Relationship to Decision-Making § 10010.47 Pre-decision referrals to CEQ. (a) Upon receipt of... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Pre-decision referrals to CEQ. 10010.47 Section 10010.47 Public Lands: Interior Regulations Relating to Public Lands (Continued) UTAH...

  19. Liability for managed care decisions: the Employee Retirement Income Security Act (ERISA) and the uneven playing field.

    PubMed

    Mariner, W K

    1996-06-01

    As managed care organizations expand their programs of quality assurance and physician evaluation, more medical malpractice lawsuits may be brought against managed care organizations on the ground that, like hospitals, they are legally responsible for negligent corporate acts that injure patients. However, the federal Employee Retirement Income Security Act (ERISA) shields managed care organizations from liability when they are part of an employee group health plan governed by ERISA. Unlike patients with other types of insurance, patients in ERISA health plans do not have a malpractice remedy for a managed care organization's negligence. A few federal appeals courts recently recognized that ERISA plans can be vicariously liable for their physicians' medical malpractice, but only if the physician is the plan's employee or agent. Yet ERISA still prohibits negligence claims against ERISA health plans for injuries resulting from denial of plan benefits, failure to use qualified physicians, utilization review, or improper plan administration. Current managed care operations do not neatly distinguish between administering benefits and controlling quality of care. Neither should the law. ERISA should be amended to provide employees with the same remedies that patients in non-ERISA plans enjoy.

  20. Ethylene acts as a negative regulator of glucose induced lateral root emergence in Arabidopsis.

    PubMed

    Singh, Manjul; Gupta, Aditi; Laxmi, Ashverya

    2015-01-01

    Plants, being sessile organisms, are more exposed to the hazards of constantly changing environmental conditions globally. During the lifetime of a plant, the root system encounters various challenges such as obstacles, pathogens, high salinity, water logging, nutrient scarcity etc. The developmental plasticity of the root system provides brilliant adaptability to plants to counter the changes exerted by both external as well as internal cues and achieve an optimized growth status. Phytohormones are one of the major intrinsic factors regulating all aspects of plant growth and development both independently as well as through complex signal integrations at multiple levels. We have previously shown that glucose (Glc) and brassinosteroid (BR) signalings interact extensively to regulate lateral root (LR) development in Arabidopsis. (1) Auxin efflux as well as influx and downstream signaling components are also involved in Glc-BR regulation of LR emergence. Here, we provide evidence for involvement of ethylene signaling machinery downstream to Glc and BR in regulation of LR emergence.

  1. Ethylene acts as a negative regulator of glucose induced lateral root emergence in Arabidopsis

    PubMed Central

    Singh, Manjul; Gupta, Aditi; Laxmi, Ashverya

    2015-01-01

    Plants, being sessile organisms, are more exposed to the hazards of constantly changing environmental conditions globally. During the lifetime of a plant, the root system encounters various challenges such as obstacles, pathogens, high salinity, water logging, nutrient scarcity etc. The developmental plasticity of the root system provides brilliant adaptability to plants to counter the changes exerted by both external as well as internal cues and achieve an optimized growth status. Phytohormones are one of the major intrinsic factors regulating all aspects of plant growth and development both independently as well as through complex signal integrations at multiple levels. We have previously shown that glucose (Glc) and brassinosteroid (BR) signalings interact extensively to regulate lateral root (LR) development in Arabidopsis.1 Auxin efflux as well as influx and downstream signaling components are also involved in Glc-BR regulation of LR emergence. Here, we provide evidence for involvement of ethylene signaling machinery downstream to Glc and BR in regulation of LR emergence. PMID:26236960

  2. The effect of joint contraceptive decisions on the use of Injectables, Long-Acting and Permanent Methods (ILAPMs) among married female (15–49) contraceptive users in Zambia: a cross-sectional study

    PubMed Central

    2014-01-01

    Background Zambia’s fertility rate and unmet need for family planning are still high. This is in spite of the progress reported from 1992 to 2007 of the increase in contraceptive prevalence rate from 15% to 41% and use of modern methods of family planning from 9% to 33%. However, partner disapproval of family planning has been cited by many women in many countries including Zambia. Given the effectiveness of long-acting and permanent methods of family planning (ILAPMs) in fertility regulation, this paper sought to examine the relationship between contraceptive decision-making and use of ILAPMs among married women in Zambia. Methods This paper uses data from the 2007 Zambia Demographic and Health Survey. The analysis is based on married women (15–49) who reported using a method of family planning at the time of the survey. Out of the 7,146 women interviewed, only 1,630 women were valid for this analysis. Cross-tabulations and binary logistic regressions with Chi-square were used to analyse associations and the predictors of use of ILAPMs of contraception, respectively. A confidence interval of .95 was used in determining relationships between independent and dependent variables. Results Two thirds of women made joint decisions regarding contraception and 29% of the women were using ILAPMs. Women who made joint contraceptive decisions are significantly more likely to use ILAPMs than women who did not involve their husband in contraceptive decisions. However, the most significant predictor is the wealth index. Women from rich households are more likely to use ILAPMs than women from medium rich and poor households. Results also show that women of North Western ethnicities and those from Region 3 had higher odds of using ILAPMs than Tonga women and women from Region 2, respectively. Conclusion Joint contraceptive decision-making between spouses is key to use of ILAPMs in Zambia. Our findings have also shown that the wealth index is actually the strongest factor

  3. 20 CFR 349.5 - Notice of revised decision.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true Notice of revised decision. 349.5 Section 349.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.5 Notice...

  4. 20 CFR 349.5 - Notice of revised decision.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Notice of revised decision. 349.5 Section 349.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.5 Notice...

  5. 20 CFR 349.6 - Effect of revised decision.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true Effect of revised decision. 349.6 Section 349.6 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.6 Effect...

  6. 20 CFR 349.5 - Notice of revised decision.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true Notice of revised decision. 349.5 Section 349.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.5 Notice...

  7. 20 CFR 349.5 - Notice of revised decision.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false Notice of revised decision. 349.5 Section 349.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.5 Notice...

  8. 20 CFR 349.5 - Notice of revised decision.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false Notice of revised decision. 349.5 Section 349.5 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.5 Notice...

  9. 20 CFR 349.6 - Effect of revised decision.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Effect of revised decision. 349.6 Section 349.6 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.6 Effect...

  10. 20 CFR 349.6 - Effect of revised decision.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false Effect of revised decision. 349.6 Section 349.6 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.6 Effect...

  11. 20 CFR 349.6 - Effect of revised decision.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false Effect of revised decision. 349.6 Section 349.6 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.6 Effect...

  12. 20 CFR 349.6 - Effect of revised decision.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true Effect of revised decision. 349.6 Section 349.6 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.6 Effect...

  13. 45 CFR 1703.201 - Decision to hold meeting.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Decision to hold meeting. 1703.201 Section 1703.201 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Procedures Governing Decisions About...

  14. 20 CFR 703.303 - Decision on employer's application.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED STATUTES INSURANCE REGULATIONS Authorization of Self-Insurers § 703.303 Decision on employer's application. (a) The Branch will issue a decision granting or denying the employer's application to be an authorized self-insurer. If the Branch grants...

  15. 20 CFR 703.303 - Decision on employer's application.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED STATUTES INSURANCE REGULATIONS Authorization of Self-Insurers § 703.303 Decision on employer's application. (a) The Branch will issue a decision granting or denying the employer's application to be an authorized self-insurer. If the Branch grants...

  16. 20 CFR 703.303 - Decision on employer's application.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED STATUTES INSURANCE REGULATIONS Authorization of Self-Insurers § 703.303 Decision on employer's application. (a) The Branch will issue a decision granting or denying the employer's application to be an authorized self-insurer. If the Branch grants...

  17. 20 CFR 703.303 - Decision on employer's application.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED STATUTES INSURANCE REGULATIONS Authorization of Self-Insurers § 703.303 Decision on employer's application. (a) The Branch will issue a decision granting or denying the employer's application to be an authorized self-insurer. If the Branch grants...

  18. 45 CFR 1203.10 - Decisions and notices.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Decisions and notices. 1203.10 Section 1203.10 Public Welfare Regulations Relating to Public Welfare (Continued) CORPORATION FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS-EFFECTUATION OF TITLE VI OF THE CIVIL RIGHTS ACT OF 1964 § 1203.10 Decisions and notices....

  19. 10 CFR 780.44 - Hearing and decision.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Hearing and decision. 780.44 Section 780.44 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153c of the Atomic Energy Act of 1954 § 780.44 Hearing and decision. In accordance with section 153d...

  20. 10 CFR 780.33 - Hearing and decision.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Hearing and decision. 780.33 Section 780.33 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.33 Hearing and decision. If any party requests a...

  1. 10 CFR 780.44 - Hearing and decision.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Hearing and decision. 780.44 Section 780.44 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153c of the Atomic Energy Act of 1954 § 780.44 Hearing and decision. In accordance with section 153d...

  2. 10 CFR 780.33 - Hearing and decision.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Hearing and decision. 780.33 Section 780.33 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.33 Hearing and decision. If any party requests a...

  3. 10 CFR 780.23 - Hearing and decision.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Hearing and decision. 780.23 Section 780.23 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Declaring Patents Affected With the Public Interest Under Section 153a of the Atomic Energy Act of 1954 § 780.23 Hearing and decision. If a...

  4. 10 CFR 780.23 - Hearing and decision.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Hearing and decision. 780.23 Section 780.23 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Declaring Patents Affected With the Public Interest Under Section 153a of the Atomic Energy Act of 1954 § 780.23 Hearing and decision. If a...

  5. 45 CFR 1703.201 - Decision to hold meeting.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....201 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Procedures Governing Decisions About Meetings... proposed meeting will ordinarily be scheduled for a date no earlier than eight days after the decision...

  6. 45 CFR 1703.201 - Decision to hold meeting.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....201 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Procedures Governing Decisions About Meetings... proposed meeting will ordinarily be scheduled for a date no earlier than eight days after the decision...

  7. 45 CFR 1703.201 - Decision to hold meeting.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....201 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Procedures Governing Decisions About Meetings... proposed meeting will ordinarily be scheduled for a date no earlier than eight days after the decision...

  8. Going-Private Decisions and the Sarbanes-Oxley Act of 2002. A Cross-Country Analysis

    DTIC Science & Technology

    2005-09-01

    example, find that the average ratio of audit fees to assets increased between 2000, the year before the Enron scandal that precipitated SOX broke, and... scandals that followed. But it was not the only response. Within the United States, courts, regulators, stock exchanges, and investors all intensified...similar reforms in other countries, paralleled SOX. 3 For example, a numerous scholars have documented how the scandals that precipitated SOX caused judges

  9. Methyl-CpG binding domain protein acts to regulate the repair of cyclobutane pyrimidine dimers on rice DNA

    PubMed Central

    Fang, Changxun; Chen, Weisi; Li, Chengxun; Jian, Xin; Li, Yingzhe; Lin, Hongmei; Lin, Wenxiong

    2016-01-01

    UVB radiation causes cyclobutane pyrimidine dimers (CPDs) to form on the DNA of living organisms. This study found that overexpression of the silicon absorbance gene Lsi1 reduced the accumulation of CPDs in rice, which profited from the reactivation by photolyase. The transcript abundance of deoxyribodipyrimidine photolyase (Os10g0167600) was generally correlated with the silicon content of the rice, and the up-regulation of Os10g0167600 was found to be highest in the UVB-treated Lsi1-overexpressed (Lsi1-OX) rice. A trans-acting factor, methyl-CpG binding domain protein (OsMeCP), was found to interact with the cis-element of Os10g0167600. The nucleic location of OsMeCP effectively enabled the transcriptional regulation. Compared with the WT, the level of OsMeCP was lower in the Lsi1-OX rice but higher in the Lsi1-RNAi line. Rice cultured in a high silicate-concentration solution also exhibited less OsMeCP abundance. Overexpression of OsMeCP led to lower Os10g0167600 transcript levels and a higher CPD content than in the WT, but the reverse was true in the OsMeCP-RNAi line. These findings indicate that OsMeCP acts as a negative regulator of silicon, and can mediate the repression of the transcription from Os10g0167600, which inhibits the photoreactivation of the photolyase involved in the repair of CPDs. PMID:27694845

  10. Past Actions as Self-Signals: How Acting in a Self-Interested Way Influences Environmental Decision Making

    PubMed Central

    Lee, Chang-Yuan; Hochman, Guy; Prince, Steven E.; Ariely, Dan

    2016-01-01

    In the last few decades, awareness of environmental issues has increased significantly. Little has changed, however, in human activities contributing to environmental damage. Why is it so difficult for us to change our behavior in a domain that is clearly so important to the future of humanity? Here we propose and test the possibility that self-signaling, the way we view ourselves based on our past behaviors, is one of the factors contributing to the difficulty of taking environmental action. In three experiments, we show that previous self-interested thoughts or behaviors serve as important signals that hinder the likelihood of acting in line with an individual’s reported concern for the environment. This study not only helps explain the gap between environmental awareness and action, but also suggests alternative strategies for policymakers and environmental agencies to promote proenvironmental behavior. PMID:27447822

  11. Disemployment effects caused by regulation of drilling fluids and produced waters as hazardous under the Resource Conservation and Recovery Act

    SciTech Connect

    Flaim, S.J.

    1988-03-01

    This report reviews and compares several studies of the effects on employment of regulating wastes from oil and natural gas exploration and extraction under the Resource Conservation and Recovery Act (RCRA). The waste management scenarios on which most of the studies were based were developed by the U.S. Environmental Protection Agency. The analyses show that as many as 500,000-700,000 jobs may be lost in the first year if RCRA Subtitle C rules are applied to drilling fluids and produced waters. As a results, unemployment in major oil-producing states could rise by as much as six percentage points. 13 refs., 4 tabs.

  12. Policy Decisions With Regard To The Applicability Of The Clean Air Act Requirements To The Strategic Petroleum Reserve

    EPA Pesticide Factsheets

    This document may be of assistance in applying the New Source Review (NSR) air permitting regulations including the Prevention of Significant Deterioration (PSD) requirements. This document is part of the NSR Policy and Guidance Database. Some documents in the database are a scanned or retyped version of a paper photocopy of the original. Although we have taken considerable effort to quality assure the documents, some may contain typographical errors. Contact the office that issued the document if you need a copy of the original.

  13. Nitric oxide acts as a positive regulator to induce metamorphosis of the ascidian Herdmania momus.

    PubMed

    Ueda, Nobuo; Degnan, Sandie M

    2013-01-01

    Marine invertebrates commonly have a biphasic life cycle in which the metamorphic transition from a pelagic larva to a benthic post-larva is mediated by the nitric oxide signalling pathway. Nitric oxide (NO) is synthesised by nitric oxide synthase (NOS), which is a client protein of the molecular chaperon heat shock protein 90 (HSP90). It is notable, then, that both NO and HSP90 have been implicated in regulating metamorphosis in marine invertebrates as diverse as urochordates, echinoderms, molluscs, annelids, and crustaceans. Specifically, the suppression of NOS activity by the application of either NOS- or HSP90-inhibiting pharmacological agents has been shown consistently to induce the initiation of metamorphosis, leading to the hypothesis that a negative regulatory role of NO is widely conserved in biphasic life cycles. Further, the induction of metamorphosis by heat-shock has been demonstrated for multiple species. Here, we investigate the regulatory role of NO in induction of metamorphosis of the solitary tropical ascidian, Herdmania momus. By coupling pharmacological treatments with analysis of HmNOS and HmHSP90 gene expression, we present compelling evidence of a positive regulatory role for NO in metamorphosis of this species, in contrast to all existing ascidian data that supports the hypothesis of NO as a conserved negative regulator of metamorphosis. The exposure of competent H. momus larvae to a NOS inhibitor or an NO donor results in an up-regulation of NOS and HSP90 genes. Heat shock of competent larvae induces metamorphosis in a temperature dependent manner, up to a thermal tolerance that approaches 35°C. Both larval/post-larval survival and the appearance of abnormal morphologies in H. momus post-larvae reflect the magnitude of up-regulation of the HSP90 gene in response to heat-shock. The demonstrated role of NO as a positive metamorphic regulator in H. momus suggests the existence of inter-specific adaptations of NO regulation in ascidian

  14. Nitric Oxide Acts as a Positive Regulator to Induce Metamorphosis of the Ascidian Herdmania momus

    PubMed Central

    Ueda, Nobuo; Degnan, Sandie M.

    2013-01-01

    Marine invertebrates commonly have a biphasic life cycle in which the metamorphic transition from a pelagic larva to a benthic post-larva is mediated by the nitric oxide signalling pathway. Nitric oxide (NO) is synthesised by nitric oxide synthase (NOS), which is a client protein of the molecular chaperon heat shock protein 90 (HSP90). It is notable, then, that both NO and HSP90 have been implicated in regulating metamorphosis in marine invertebrates as diverse as urochordates, echinoderms, molluscs, annelids, and crustaceans. Specifically, the suppression of NOS activity by the application of either NOS- or HSP90-inhibiting pharmacological agents has been shown consistently to induce the initiation of metamorphosis, leading to the hypothesis that a negative regulatory role of NO is widely conserved in biphasic life cycles. Further, the induction of metamorphosis by heat-shock has been demonstrated for multiple species. Here, we investigate the regulatory role of NO in induction of metamorphosis of the solitary tropical ascidian, Herdmania momus. By coupling pharmacological treatments with analysis of HmNOS and HmHSP90 gene expression, we present compelling evidence of a positive regulatory role for NO in metamorphosis of this species, in contrast to all existing ascidian data that supports the hypothesis of NO as a conserved negative regulator of metamorphosis. The exposure of competent H. momus larvae to a NOS inhibitor or an NO donor results in an up-regulation of NOS and HSP90 genes. Heat shock of competent larvae induces metamorphosis in a temperature dependent manner, up to a thermal tolerance that approaches 35°C. Both larval/post-larval survival and the appearance of abnormal morphologies in H. momus post-larvae reflect the magnitude of up-regulation of the HSP90 gene in response to heat-shock. The demonstrated role of NO as a positive metamorphic regulator in H. momus suggests the existence of inter-specific adaptations of NO regulation in ascidian

  15. 45 CFR 681.36 - When does the initial decision of the ALJ become final?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... final? 681.36 Section 681.36 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.36 When does... decision of NSF, and shall be binding on all parties 30 days after it is issued, unless any party...

  16. 45 CFR 681.36 - When does the initial decision of the ALJ become final?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... final? 681.36 Section 681.36 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.36 When does... decision of NSF, and shall be binding on all parties 30 days after it is issued, unless any party...

  17. 45 CFR 681.37 - What are the procedures for appealing the ALJ decision?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... decision? 681.37 Section 681.37 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.37 What are... defendant may file a notice of appeal with the authority head within 30 days following issuance of...

  18. 45 CFR 681.37 - What are the procedures for appealing the ALJ decision?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... decision? 681.37 Section 681.37 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.37 What are... defendant may file a notice of appeal with the authority head within 30 days following issuance of...

  19. 45 CFR 681.36 - When does the initial decision of the ALJ become final?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... final? 681.36 Section 681.36 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.36 When does... decision of NSF, and shall be binding on all parties 30 days after it is issued, unless any party...

  20. 45 CFR 681.37 - What are the procedures for appealing the ALJ decision?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... decision? 681.37 Section 681.37 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 681.37 What are... defendant may file a notice of appeal with the authority head within 30 days following issuance of...

  1. Cued to Act on Impulse: More Impulsive Choice and Risky Decision Making by Women Susceptible to Overeating after Exposure to Food Stimuli

    PubMed Central

    Yeomans, Martin R.; Brace, Aaron

    2015-01-01

    There is increasing evidence that individual differences in tendency to overeat relate to impulsivity, possibly by increasing reactivity to food-related cues in the environment. This study tested whether acute exposure to food cues enhanced impulsive and risky responses in women classified on tendency to overeat, indexed by scores on the three factor eating questionnaire disinhibition (TFEQ-D), restraint (TFEQ-R) and hunger scales. Ninety six healthy women completed two measures of impulsive responding (delayed discounting, DDT and a Go No-Go, GNG, task) and a measure of risky decision making (the balloon analogue risk task, BART) as well as questionnaire measures of impulsive behaviour either after looking at a series of pictures of food or visually matched controls. Impulsivity (DDT) and risk-taking (BART) were both positively associated with TFEQ-D scores, but in both cases this effect was exacerbated by prior exposure to food cues. No effects of restraint were found. TFEQ-D scores were also related to more commission errors on the GNG, while restrained women were slower on the GNG, but neither effect was modified by cue exposure. Overall these data suggest that exposure to food cues act to enhance general impulsive responding in women at risk of overeating and tentatively suggest an important interaction between tendency for impulsive decision making and food cues that may help explain a key underlying risk factor for overeating. PMID:26378459

  2. SlgA, the homologue of the human schizophrenia associated PRODH gene, acts in clock neurons to regulate Drosophila aggression.

    PubMed

    Zwarts, Liesbeth; Vulsteke, Veerle; Buhl, Edgar; Hodge, James J L; Callaerts, Patrick

    2017-03-22

    Mutations in proline dehydrogenase (PRODH) are linked to behavioral alterations in schizophrenia and as part of DiGeorge and velo-cardio-facial syndromes, but the role of PRODH in their etiology remains unclear. We here establish a Drosophila model to study the role of PRODH in behavioral disorders. We determine the distribution of the Drosophila PRODH homolog slgA in the brain and show that knock-down and overexpression of human PRODH and slgA in the lateral neurons ventral (LNv) lead to altered aggressive behavior. SlgA acts in an isoform-specific manner and is regulated by casein kinase II (CkII). Our data suggest that these effects are, at least partially, due to effects on mitochondrial function. We thus show that precise regulation of proline metabolism is essential to drive normal behavior and we identify Drosophila aggression as a model behavior relevant for the study of mechanisms impaired in neuropsychiatric disorders.

  3. Potential Impact of Clean Air Act Regulations on Nitrogen Fate and Transport in the Neuse River Basin: a Modeling Investigation Using CMAQ and SWAT

    EPA Science Inventory

    There has been extensive analysis of Clean Air Act Amendment (CAAA) regulation impacts to changes in atmospheric nitrogen deposition; however, few studies have focused on watershed nitrogen transfer particularly regarding long-term predictions. In this study, we investigated impa...

  4. 75 FR 19241 - Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations; Defining...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... Service lacks the authority to examine for compliance with the BSA. The definition of ``mutual fund'' at... funds within the general definition of ``financial institution'' in regulations implementing the Bank... information for transmittals of funds. Additionally, the final rule amends the definition of mutual fund...

  5. 76 FR 52295 - Amendment of Privacy Act Regulations, Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-22

    ...; --Use the active voice to address readers directly; --Use clear language rather than jargon; --Be... relevance and necessity to accomplish a purpose of the agency will be clear. 4. From subsections (e)(4)(G... the criteria of section 3(b)(2) requiring that all regulations be written in clear language...

  6. 75 FR 66699 - Lacey Act Implementation Plan; Definitions for Exempt and Regulated Articles

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-29

    ... Implementation Plan; Definitions for Exempt and Regulated Articles AGENCY: Animal and Plant Health Inspection... period for our proposed rule that would establish definitions for the terms ``common cultivar'' and... definitions for the terms ``common cultivar'' and ``common food crop.'' We proposed to establish...

  7. 78 FR 27308 - Loan Originator Compensation Requirements Under the Truth In Lending Act (Regulation Z...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-10

    ... implementing regulations by that date.\\2\\ To avoid uncertainty and potential disruption in the national... rules, as needed; (4) publication of readiness guides for the new rules; and (5) education of consumers... disruptions in the provision of credit insurance products to consumers while interpretive questions...

  8. 75 FR 50718 - Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-17

    ... measured by excluding from the measurement certain attached structures. Currently, the regulations state that ``length means a straight line measurement of the overall length from the foremost part of the vessel to the aftmost part of the vessel, measured parallel to the center line. The measurement shall...

  9. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes... 40 Protection of Environment 25 2010-07-01 2010-07-01 false PCB wastes regulated under Toxic... hazardous only because they fail the test for the Toxicity Characteristic (Hazardous Waste Codes...

  10. 77 FR 76861 - Removal of Job Training Partnership Act Implementing Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ... accessible to the public. Therefore, the Department recommends that commenters remove personal information such as Social Security Numbers, personal addresses, telephone numbers, and email addresses included in....regulations.gov Web site. It is the responsibility of the commenter to safeguard their personal...

  11. The Patient Protection and Affordable Care Act and the regulation of the health insurance industry.

    PubMed

    Jha, Saurabh; Baker, Tom

    2012-12-01

    The Patient Protection and Affordable Care Act is a comprehensive and multipronged reform of the US health care system. The legislation makes incremental changes to Medicare, Medicaid, and the market for employer-sponsored health insurance. However, it makes substantial changes to the market for individual and small-group health insurance. The purpose of this article is to introduce the key regulatory reforms in the market for individual and small-group health insurance and explain how these reforms tackle adverse selection and risk classification and improve access to health care for the hitherto uninsured or underinsured population.

  12. GIGANTEA acts in blue light signaling and has biochemically separable roles in circadian clock and flowering time regulation.

    PubMed

    Martin-Tryon, Ellen L; Kreps, Joel A; Harmer, Stacey L

    2007-01-01

    Circadian clocks are widespread in nature. In higher plants, they confer a selective advantage, providing information regarding not only time of day but also time of year. Forward genetic screens in Arabidopsis (Arabidopsis thaliana) have led to the identification of many clock components, but the functions of most of these genes remain obscure. To identify both new constituents of the circadian clock and new alleles of known clock-associated genes, we performed a mutant screen. Using a clock-regulated luciferase reporter, we isolated new alleles of ZEITLUPE, LATE ELONGATED HYPOCOTYL, and GIGANTEA (GI). GI has previously been reported to function in red light signaling, central clock function, and flowering time regulation. Characterization of this and other GI alleles has helped us to further define GI function in the circadian system. We found that GI acts in photomorphogenic and circadian blue light signaling pathways and is differentially required for clock function in constant red versus blue light. Gene expression and epistasis analyses show that TIMING OF CHLOROPHYLL A/B BINDING PROTEIN1 (TOC1) expression is not solely dependent upon GI and that GI expression is only indirectly affected by TOC1, suggesting that GI acts both in series with and in parallel to TOC1 within the central circadian oscillator. Finally, we found that the GI-dependent promotion of CONSTANS expression and flowering is intact in a gi mutant with altered circadian regulation. Thus GI function in the regulation of a clock output can be biochemically separated from its role within the circadian clock.

  13. An extracytoplasmic function sigma factor acts as a general stress response regulator in Sinorhizobium meliloti.

    PubMed

    Sauviac, Laurent; Philippe, Heinui; Phok, Kounthéa; Bruand, Claude

    2007-06-01

    Sinorhizobium meliloti genes transcriptionally up-regulated after heat stress, as well as upon entry into stationary phase, were identified by microarray analyses. Sixty stress response genes were thus found to be up-regulated under both conditions. One of them, rpoE2 (smc01506), encodes a putative extracytoplasmic function (ECF) sigma factor. We showed that this sigma factor controls its own transcription and is activated by various stress conditions, including heat and salt, as well as entry into stationary phase after either carbon or nitrogen starvation. We also present evidence that the product of the gene cotranscribed with rpoE2 negatively regulates RpoE2 activity, and we therefore propose that it plays the function of anti-sigma factor. By combining transcriptomic, bioinformatic, and quantitative reverse transcription-PCR analyses, we identified 44 RpoE2-controlled genes and predicted the number of RpoE2 targets to be higher. Strikingly, more than one-third of the 60 stress response genes identified in this study are RpoE2 targets. Interestingly, two genes encoding proteins with known functions in stress responses, namely, katC and rpoH2, as well as a second ECF-encoding gene, rpoE5, were found to be RpoE2 regulated. Altogether, these data suggest that RpoE2 is a major global regulator of the general stress response in S. meliloti. Despite these observations, and although this sigma factor is well conserved among alphaproteobacteria, no in vitro nor in planta phenotypic difference from the wild-type strain could be detected for rpoE2 mutants. This therefore suggests that other important actors in the general stress response have still to be identified in S. meliloti.

  14. Sexually dimorphic control of gene expression in sensory neurons regulates decision-making behavior in C. elegans

    PubMed Central

    Hilbert, Zoë A; Kim, Dennis H

    2017-01-01

    Animal behavior is directed by the integration of sensory information from internal states and the environment. Neuroendocrine regulation of diverse behaviors of Caenorhabditis elegans is under the control of the DAF-7/TGF-β ligand that is secreted from sensory neurons. Here, we show that C. elegans males exhibit an altered, male-specific expression pattern of daf-7 in the ASJ sensory neuron pair with the onset of reproductive maturity, which functions to promote male-specific mate-searching behavior. Molecular genetic analysis of the switch-like regulation of daf-7 expression in the ASJ neuron pair reveals a hierarchy of regulation among multiple inputs—sex, age, nutritional status, and microbial environment—which function in the modulation of behavior. Our results suggest that regulation of gene expression in sensory neurons can function in the integration of a wide array of sensory information and facilitate decision-making behaviors in C. elegans. DOI: http://dx.doi.org/10.7554/eLife.21166.001 PMID:28117661

  15. Regulating dangerous futures: the German Embryo Protection Act of 1990--legislation in risk society.

    PubMed

    Augst, C

    2000-06-01

    This article summarises the outcome of a research project which analyses the legislative debate about the German Embryonenschutzgesetz (Embryo Protection Act) in 1990. From 1988 to 1990 the German Parliament discussed legislation for the practice of assisted contraception and embryo research. The term 'risk' is central to the discourse. For Ulrich Beck (1986) this emphasis on risk is a sign of the reflexivity which contemporary western societies have reached. This article reads back into the risk discourse the values hidden in risk terminology: they are identified as fears about modernisation processes. The focus on risk in this article allows observation of late modernity's unease about its own potential and a growing ambiguity about modern ideas of progress and control (Bauman, 1991). This ambiguity also becomes apparent in the strategies of policing which the German legislature offers as solutions to the perceived risks: different legislative strategies are developed to tackle the contradictory risk scenarios. These different strategies of policing are understood as the construction of 'places of safety' in the face of identified dangers: the 'traditional family', the 'good doctor', 'professional' judgement. Defining those boundaries allows the German legislature to juggle contradictory agendas. This explains the inconsistent and fragmented nature of the Embryo Protection Act 1990.

  16. 20 CFR 349.1 - Reopening and revising decisions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Section 349.1 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.1 Reopening... with respect to benefits under the Railroad Unemployment Insurance Act. After the expiration of...

  17. 20 CFR 349.1 - Reopening and revising decisions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Section 349.1 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.1 Reopening... with respect to benefits under the Railroad Unemployment Insurance Act. After the expiration of...

  18. 20 CFR 349.1 - Reopening and revising decisions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Section 349.1 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.1 Reopening... with respect to benefits under the Railroad Unemployment Insurance Act. After the expiration of...

  19. 20 CFR 349.1 - Reopening and revising decisions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Section 349.1 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.1 Reopening... with respect to benefits under the Railroad Unemployment Insurance Act. After the expiration of...

  20. 20 CFR 349.1 - Reopening and revising decisions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Section 349.1 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.1 Reopening... with respect to benefits under the Railroad Unemployment Insurance Act. After the expiration of...

  1. 16 CFR 700.8 - Warrantor's decision as final.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Warrantor's decision as final. 700.8 Section 700.8 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE MAGNUSON-MOSS WARRANTY ACT INTERPRETATIONS OF MAGNUSON-MOSS WARRANTY ACT § 700.8...

  2. 16 CFR 700.8 - Warrantor's decision as final.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Warrantor's decision as final. 700.8 Section 700.8 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE MAGNUSON-MOSS WARRANTY ACT INTERPRETATIONS OF MAGNUSON-MOSS WARRANTY ACT § 700.8...

  3. 16 CFR 700.8 - Warrantor's decision as final.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Warrantor's decision as final. 700.8 Section 700.8 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE MAGNUSON-MOSS WARRANTY ACT INTERPRETATIONS OF MAGNUSON-MOSS WARRANTY ACT § 700.8...

  4. 16 CFR 700.8 - Warrantor's decision as final.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Warrantor's decision as final. 700.8 Section 700.8 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE MAGNUSON-MOSS WARRANTY ACT INTERPRETATIONS OF MAGNUSON-MOSS WARRANTY ACT § 700.8...

  5. 16 CFR 700.8 - Warrantor's decision as final.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Warrantor's decision as final. 700.8 Section 700.8 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE MAGNUSON-MOSS WARRANTY ACT INTERPRETATIONS OF MAGNUSON-MOSS WARRANTY ACT § 700.8...

  6. The multi zinc-finger protein Trps1 acts as a regulator of histone deacetylation during mitosis.

    PubMed

    Wuelling, Manuela; Pasdziernik, Markus; Moll, Carina N; Thiesen, Andrea M; Schneider, Sabine; Johannes, Christian; Vortkamp, Andrea

    2013-07-15

    TRPS1, the gene mutated in human "Tricho-Rhino-Phalangeal syndrome," encodes a multi zinc-finger nuclear regulator of chondrocyte proliferation and differentiation. Here, we have identified a new function of Trps1 in controlling mitotic progression in chondrocytes. Loss of Trps1 in mice leads to an increased proportion of cells arrested in mitosis and, subsequently, to chromosome segregation defects. Searching for the molecular basis of the defect, we found that Trps1 acts as regulator of histone deacetylation. Trps1 interacts with two histone deacetylases, Hdac1 and Hdac4, thereby increasing their activity. Loss of Trps1 results in histone H3 hyperacetylation, which is maintained during mitosis. Consequently, chromatin condensation and binding of HP1 is impaired, and Trps1-deficient chondrocytes accumulate in prometaphase. Overexpression of Hdac4 rescues the mitotic defect of Trps1-deficient chondrocytes, identifying Trps1 as an important regulator of chromatin deacetylation during mitosis in chondrocytes. Our data provide the first evidence that the control of mitosis can be linked to the regulation of chondrocyte differentiation by epigenetic consequences of altered Hdac activity.

  7. CDH2 and CDH11 as Regulators of Stem Cell Fate Decisions

    PubMed Central

    Alimperti, Stella; Andreadis, Stelios T.

    2015-01-01

    Accumulating evidence suggests that the mechanical and biochemical signals originating from cell-cell adhesion are critical for stem cell lineage specification. In this review, we focus on the role of cadherin mediated signaling in development and stem cell differentiation, with emphasis on two well-known cadherins, cadherin-2 (CDH2) (N-cadherin) and cadherin-11 (CDH11) (OB-cadherin). We summarize the existing knowledge regarding the role of CDH2 and CDH11 during development and differentiation in vivo and in vitro. We also discuss engineering strategies to control stem cell fate decisions by fine-tuning the extent of cell-cell adhesion through surface chemistry and microtopology. These studies may be greatly facilitated by novel strategies that enable monitoring of stem cell specification in real time. We expect that better understanding of how intercellular adhesion signaling affects lineage specification may impact biomaterial and scaffold design to control stem cell fate decisions in three-dimensional context with potential implications for tissue engineering and regenerative medicine. PMID:25771201

  8. Definition of spouse under the Family and Medical Leave Act. Final rule.

    PubMed

    2015-02-25

    The Department of Labor's (Department) Wage and Hour Division (WHD) revises the regulation defining "spouse" under the Family and Medical Leave Act of 1993 (FMLA or the Act) in light of the United States Supreme Court's decision in United States v. Windsor, which found section 3 of the Defense of Marriage Act (DOMA) to be unconstitutional.

  9. An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development.

    PubMed

    Chae, Eunyoung; Tan, Queenie K-G; Hill, Theresa A; Irish, Vivian F

    2008-04-01

    Plants flower in response to both environmental and endogenous signals. The Arabidopsis LEAFY (LFY) transcription factor is crucial in integrating these signals, and acts in part by activating the expression of multiple floral homeotic genes. LFY-dependent activation of the homeotic APETALA3 (AP3) gene requires the activity of UNUSUAL FLORAL ORGANS (UFO), an F-box component of an SCF ubiquitin ligase, yet how this regulation is effected has remained unclear. Here, we show that UFO physically interacts with LFY both in vitro and in vivo, and this interaction is necessary to recruit UFO to the AP3 promoter. Furthermore, a transcriptional repressor domain fused to UFO reduces endogenous LFY activity in plants, supporting the idea that UFO acts as part of a transcriptional complex at the AP3 promoter. Moreover, chemical or genetic disruption of proteasome activity compromises LFY-dependent AP3 activation, indicating that protein degradation is required to promote LFY activity. These results define an unexpected role for an F-box protein in functioning as a DNA-associated transcriptional co-factor in regulating floral homeotic gene expression. These results suggest a novel mechanism for promoting flower development via protein degradation and concomitant activation of the LFY transcription factor. This mechanism may be widely conserved, as homologs of UFO and LFY have been identified in a wide array of plant species.

  10. Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance

    PubMed Central

    Tsang, Chi Kwan; Liu, Yuan; Thomas, Janice; Zhang, Yanjie; Zheng, X. F. Steven

    2015-01-01

    Summary Superoxide dismutase 1 (Sod1) has been known for nearly half a century for catalysis of superoxide to hydrogen peroxide. Here we report a new Sod1 function in oxidative signaling: in response to elevated endogenous and exogenous reactive oxygen species (ROS), Sod1 rapidly relocates into the nucleus, which is important for maintaining genomic stability. Interestingly, H2O2 is sufficient to promote Sod1 nuclear localization, indicating that it is responding to general ROS rather than Sod1 substrate superoxide. ROS signaling is mediated by Mec1/ATM and its effector Dun1/Cds1 kinase, through Dun1 interaction with Sod1 and regulation of Sod1 by phosphorylation at S60, 99. In the nucleus, Sod1 binds to the promoters and regulates the expression of oxidative resistance and repair genes. Altogether, our study unravels an unorthodox function of Sod1 as a transcription factor and elucidates the regulatory mechanism for its localization. PMID:24647101

  11. Two Group A Streptococcal Peptide Pheromones Act through Opposing Rgg Regulators to Control Biofilm Development

    PubMed Central

    Chang, Jennifer C.; LaSarre, Breah; Jimenez, Juan C.; Aggarwal, Chaitanya; Federle, Michael J.

    2011-01-01

    Streptococcus pyogenes (Group A Streptococcus, GAS) is an important human commensal that occasionally causes localized infections and less frequently causes severe invasive disease with high mortality rates. How GAS regulates expression of factors used to colonize the host and avoid immune responses remains poorly understood. Intercellular communication is an important means by which bacteria coordinate gene expression to defend against host assaults and competing bacteria, yet no conserved cell-to-cell signaling system has been elucidated in GAS. Encoded within the GAS genome are four rgg-like genes, two of which (rgg2 and rgg3) have no previously described function. We tested the hypothesis that rgg2 or rgg3 rely on extracellular peptides to control target-gene regulation. We found that Rgg2 and Rgg3 together tightly regulate two linked genes encoding new peptide pheromones. Rgg2 activates transcription of and is required for full induction of the pheromone genes, while Rgg3 plays an antagonistic role and represses pheromone expression. The active pheromone signals, termed SHP2 and SHP3, are short and hydrophobic (DI[I/L]IIVGG), and, though highly similar in sequence, their ability to disrupt Rgg3-DNA complexes were observed to be different, indicating that specificity and differential activation of promoters are characteristics of the Rgg2/3 regulatory circuit. SHP-pheromone signaling requires an intact oligopeptide permease (opp) and a metalloprotease (eep), supporting the model that pro-peptides are secreted, processed to the mature form, and subsequently imported to the cytoplasm to interact directly with the Rgg receptors. At least one consequence of pheromone stimulation of the Rgg2/3 pathway is increased biogenesis of biofilms, which counteracts negative regulation of biofilms by RopB (Rgg1). These data provide the first demonstration that Rgg-dependent quorum sensing functions in GAS and substantiate the role that Rggs play as peptide receptors across the

  12. Two group A streptococcal peptide pheromones act through opposing Rgg regulators to control biofilm development.

    PubMed

    Chang, Jennifer C; LaSarre, Breah; Jimenez, Juan C; Aggarwal, Chaitanya; Federle, Michael J

    2011-08-01

    Streptococcus pyogenes (Group A Streptococcus, GAS) is an important human commensal that occasionally causes localized infections and less frequently causes severe invasive disease with high mortality rates. How GAS regulates expression of factors used to colonize the host and avoid immune responses remains poorly understood. Intercellular communication is an important means by which bacteria coordinate gene expression to defend against host assaults and competing bacteria, yet no conserved cell-to-cell signaling system has been elucidated in GAS. Encoded within the GAS genome are four rgg-like genes, two of which (rgg2 and rgg3) have no previously described function. We tested the hypothesis that rgg2 or rgg3 rely on extracellular peptides to control target-gene regulation. We found that Rgg2 and Rgg3 together tightly regulate two linked genes encoding new peptide pheromones. Rgg2 activates transcription of and is required for full induction of the pheromone genes, while Rgg3 plays an antagonistic role and represses pheromone expression. The active pheromone signals, termed SHP2 and SHP3, are short and hydrophobic (DI[I/L]IIVGG), and, though highly similar in sequence, their ability to disrupt Rgg3-DNA complexes were observed to be different, indicating that specificity and differential activation of promoters are characteristics of the Rgg2/3 regulatory circuit. SHP-pheromone signaling requires an intact oligopeptide permease (opp) and a metalloprotease (eep), supporting the model that pro-peptides are secreted, processed to the mature form, and subsequently imported to the cytoplasm to interact directly with the Rgg receptors. At least one consequence of pheromone stimulation of the Rgg2/3 pathway is increased biogenesis of biofilms, which counteracts negative regulation of biofilms by RopB (Rgg1). These data provide the first demonstration that Rgg-dependent quorum sensing functions in GAS and substantiate the role that Rggs play as peptide receptors across the

  13. Phosphorylation acts positively and negatively to regulate MRTF-A subcellular localisation and activity

    PubMed Central

    Panayiotou, Richard; Miralles, Francesc; Pawlowski, Rafal; Diring, Jessica; Flynn, Helen R; Skehel, Mark; Treisman, Richard

    2016-01-01

    The myocardin-related transcription factors (MRTF-A and MRTF-B) regulate cytoskeletal genes through their partner transcription factor SRF. The MRTFs bind G-actin, and signal-regulated changes in cellular G-actin concentration control their nuclear accumulation. The MRTFs also undergo Rho- and ERK-dependent phosphorylation, but the function of MRTF phosphorylation, and the elements and signals involved in MRTF-A nuclear export are largely unexplored. We show that Rho-dependent MRTF-A phosphorylation reflects relief from an inhibitory function of nuclear actin. We map multiple sites of serum-induced phosphorylation, most of which are S/T-P motifs and show that S/T-P phosphorylation is required for transcriptional activation. ERK-mediated S98 phosphorylation inhibits assembly of G-actin complexes on the MRTF-A regulatory RPEL domain, promoting nuclear import. In contrast, S33 phosphorylation potentiates the activity of an autonomous Crm1-dependent N-terminal NES, which cooperates with five other NES elements to exclude MRTF-A from the nucleus. Phosphorylation thus plays positive and negative roles in the regulation of MRTF-A. DOI: http://dx.doi.org/10.7554/eLife.15460.001 PMID:27304076

  14. Centriolar SAS-7 acts upstream of SPD-2 to regulate centriole assembly and pericentriolar material formation.

    PubMed

    Sugioka, Kenji; Hamill, Danielle R; Lowry, Joshua B; McNeely, Marie E; Enrick, Molly; Richter, Alyssa C; Kiebler, Lauren E; Priess, James R; Bowerman, Bruce

    2017-01-16

    The centriole/basal body is a eukaryotic organelle that plays essential roles in cell division and signaling. Among five known core centriole proteins, SPD-2/Cep192 is the first recruited to the site of daughter centriole formation and regulates the centriolar localization of the other components in C. elegans and in humans. However, the molecular basis for SPD-2 centriolar localization remains unknown. Here we describe a new centriole component, the coiled-coil protein SAS-7, as a regulator of centriole duplication, assembly and elongation. We also show that SAS-7 binds SPD-2 and regulates SPD-2 centriolar recruitment, while SAS-7 centriolar localization is SPD-2-independent. Furthermore, pericentriolar material (PCM) formation is abnormal in sas-7 mutants, and the PCM-dependent induction of cell polarity that defines the anterior-posterior body axis frequently fails. We conclude that SAS-7 functions at the earliest step in centriole duplication yet identified and plays important roles in the orchestration of centriole and PCM assembly.

  15. FOXL2 and BMP2 Act Cooperatively to Regulate Follistatin Gene Expression during Ovarian Development

    PubMed Central

    Kashimada, Kenichi; Pelosi, Emanuele; Chen, Huijun; Schlessinger, David; Wilhelm, Dagmar; Koopman, Peter

    2011-01-01

    Follistatin is a secreted glycoprotein required for female sex determination and early ovarian development, but the precise mechanisms regulating follistatin (Fst) gene expression are not known. Here, we investigate the roles of bone morphogenetic protein 2 (BMP2) and forkhead-domain transcription factor L2 (FOXL2) in the regulation of Fst expression in the developing mouse ovary. Bmp2 and Fst showed similar temporal profiles of mRNA expression, whereas FOXL2 protein and Fst mRNA were coexpressed in the same ovarian cells. In a cell culture model, both FOXL2 and BMP2 up-regulated Fst expression. In ex vivo mouse fetal gonad culture, exogenous BMP2 increased Fst expression, but this effect was counteracted by the BMP antagonist Noggin. Moreover, in Foxl2-null mice, Fst expression was reduced throughout fetal ovarian development, and Bmp2 expression was also reduced. Our data support a model in which FOXL2 and BMP2 cooperate to ensure correct expression of Fst in the developing ovary. Further, Wnt4-knockout mice showed reduced expression of Fst limited to early ovarian development, suggesting a role for WNT4 in the initiation, but not the maintenance, of Fst expression. PMID:21084449

  16. Centriolar SAS-7 acts upstream of SPD-2 to regulate centriole assembly and pericentriolar material formation

    PubMed Central

    Sugioka, Kenji; Hamill, Danielle R; Lowry, Joshua B; McNeely, Marie E; Enrick, Molly; Richter, Alyssa C; Kiebler, Lauren E; Priess, James R; Bowerman, Bruce

    2017-01-01

    The centriole/basal body is a eukaryotic organelle that plays essential roles in cell division and signaling. Among five known core centriole proteins, SPD-2/Cep192 is the first recruited to the site of daughter centriole formation and regulates the centriolar localization of the other components in C. elegans and in humans. However, the molecular basis for SPD-2 centriolar localization remains unknown. Here, we describe a new centriole component, the coiled-coil protein SAS-7, as a regulator of centriole duplication, assembly and elongation. Intriguingly, our genetic data suggest that SAS-7 is required for daughter centrioles to become competent for duplication, and for mother centrioles to maintain this competence. We also show that SAS-7 binds SPD-2 and regulates SPD-2 centriolar recruitment, while SAS-7 centriolar localization is SPD-2-independent. Furthermore, pericentriolar material (PCM) formation is abnormal in sas-7 mutants, and the PCM-dependent induction of cell polarity that defines the anterior-posterior body axis frequently fails. We conclude that SAS-7 functions at the earliest step in centriole duplication yet identified and plays important roles in the orchestration of centriole and PCM assembly. DOI: http://dx.doi.org/10.7554/eLife.20353.001 PMID:28092264

  17. Identification of cis- and trans-acting elements regulating calretinin expression in mesothelioma cells

    PubMed Central

    Kresoja-Rakic, Jelena; Kapaklikaya, Esra; Ziltener, Gabriela; Dalcher, Damian; Santoro, Raffaella; Christensen, Brock C.; Johnson, Kevin C.; Schwaller, Beat; Weder, Walter; Stahel, Rolf A.; Felley-Bosco, Emanuela

    2016-01-01

    Calretinin (CALB2) is a diagnostic marker for epithelioid mesothelioma. It is also a prognostic marker since patients with tumors expressing high calretinin levels have better overall survival. Silencing of calretinin decreases viability of epithelioid mesothelioma cells. Our aim was to elucidate mechanisms regulating calretinin expression in mesothelioma. Analysis of calretinin transcript and protein suggested a control at the mRNA level. Treatment with 5-aza-2′-deoxycytidine and analysis of TCGA data indicated that promoter methylation is not likely to be involved. Therefore, we investigated CALB2 promoter by analyzing ~1kb of genomic sequence surrounding the transcription start site (TSS) + 1 using promoter reporter assay. Deletion analysis of CALB2 proximal promoter showed that sequence spanning the −161/+80bp region sustained transcriptional activity. Site-directed analysis identified important cis-regulatory elements within this −161/+80bp CALB2 promoter. EMSA and ChIP assays confirmed binding of NRF-1 and E2F2 to the CALB2 promoter and siRNA knockdown of NRF-1 led to decreased expression of calretinin. Cell synchronization experiment showed that calretinin expression was cell cycle regulated with a peak of expression at G1/S phase. This study provides the first insight in the regulation of CALB2 expression in mesothelioma cells. PMID:26848772

  18. The Texas space flight liability act and efficient regulation for the private commercial space flight era

    NASA Astrophysics Data System (ADS)

    Johnson, Christopher D.

    2013-12-01

    In the spring of 2011, the American state of Texas passed into law an act limiting the liability of commercial space flight entities. Under it, those companies would not be liable for space flight participant injuries, except in cases of intentional injury or injury proximately caused by the company's gross negligence. An analysis within the framework of international and national space law, but especially informed by the academic discipline of law and economics, discusses the incentives of all relevant parties and attempts to understand whether the law is economically "efficient" (allocating resources so as to yield maximum utility), and suited to further the development of the fledgling commercial suborbital tourism industry. Insights into the Texas law are applicable to other states hoping to foster commercial space tourism and considering space tourism related legislation.

  19. The ZEB1 Transcription Factor Acts in a Negative Feedback Loop with miR200 Downstream of Ras and Rb1 to Regulate Bmi1 Expression*

    PubMed Central

    Liu, Yongqing; Sánchez-Tilló, Ester; Lu, Xiaoqin; Huang, Li; Clem, Brian; Telang, Sucheta; Jenson, Alfred B.; Cuatrecasas, Miriam; Chesney, Jason; Postigo, Antonio; Dean, Douglas C.

    2014-01-01

    Ras mutations are frequent in cancer cells where they drive proliferation and resistance to apoptosis. However in primary cells, mutant Ras instead can cause oncogene-induced senescence, a tumor suppressor function linked to repression of the polycomb factor Bmi1, which normally regulates cell cycle inhibitory cyclin-dependent kinase inhibitors (cdki). It is unclear how Ras causes repression of Bmi1 in primary cells to suppress tumor formation while inducing the gene in cancer cells to drive tumor progression. Ras also induces the EMT transcription factor ZEB1 to trigger tumor invasion and metastasis. Beyond its well-documented role in EMT, ZEB1 is important for maintaining repression of cdki. Indeed, heterozygous mutation of ZEB1 is sufficient for elevated cdki expression, leading to premature senescence of primary cells. A similar phenotype is evident with Bmi1 mutation. We show that activation of Rb1 in response to mutant Ras causes dominant repression of ZEB1 in primary cells, but loss of the Rb1 pathway is a hallmark of cancer cells and in the absence of such Rb1 repression Ras induces ZEB1 in cancer cells. ZEB1 represses miR-200 in the context of a mutual repression loop. Because miR-200 represses Bmi1, induction of ZEB1 leads to induction of Bmi1. Rb1 pathway status then dictates the opposing effects of mutant Ras on the ZEB1-miR-200 loop in primary versus cancer cells. This loop not only triggers EMT, surprisingly we show it acts downstream of Ras to regulate Bmi1 expression and thus the critical decision between oncogene-induced senescence and tumor initiation. PMID:24371144

  20. Neurotransmitters act as paracrine signals to regulate insulin secretion from the human pancreatic islet.

    PubMed

    Rodriguez-Diaz, Rayner; Menegaz, Danusa; Caicedo, Alejandro

    2014-08-15

    In this symposium review we discuss the role of neurotransmitters as paracrine signals that regulate pancreatic islet function. A large number of neurotransmitters and their receptors has been identified in the islet, but relatively little is known about their involvement in islet biology. Interestingly, neurotransmitters initially thought to be present in autonomic axons innervating the islet are also present in endocrine cells of the human islet. These neurotransmitters can thus be released as paracrine signals to help control hormone release. Here we propose that the role of neurotransmitters may extend beyond controlling endocrine cell function to work as signals modulating vascular flow and immune responses within the islet.

  1. Employee Retirement Income Security Act of 1974; rules and regulations for administration and enforcement; claims procedure. Final regulation; delay of applicability date.

    PubMed

    2001-07-09

    This action delays for at least six months and not more than one year the applicability date for the regulation governing minimum requirements for benefit claims procedures of group health plans covered by Title I of the Employee Retirement Income Security Act. As published on November 21, 2000, the benefit claims procedure would be applicable to claims filed on or after January 1, 2002. The current action amends the regulation so that it will apply to group health claims filed on or after the first day of the first plan year beginning on or after July 1, 2002, but in no event later than January 1, 2003. This action provides a limited additional period within which group health plan sponsors, administrators, and service providers can bring their claims processing systems into compliance with the new requirements. A postponement of the applicability date with respect to group health claims will allow a more orderly transition to the new standards and will avoid the confusion and additional expense that would be caused if certain pending Congressional bills are enacted before or soon after the original applicability date. This action does not apply to pension plans or plans providing disability or welfare benefits (other than group health). For these plans, the regulation will continue to be applicable to claims filed on or after January 1, 2002.

  2. CBX7 and HMGA1b proteins act in opposite way on the regulation of the SPP1 gene expression

    PubMed Central

    Sepe, Romina; Formisano, Umberto; Federico, Antonella; Forzati, Floriana; Bastos, André Uchimura; D'Angelo, Daniela; Cacciola, Nunzio Antonio; Fusco, Alfredo; Pallante, Pierlorenzo

    2015-01-01

    Several recent studies have reported the Polycomb Repressive Complex 1 member CBX7 as a tumor-suppressor gene whose expression progressively decreases in different human carcinomas in relation with tumor grade, malignant stage and poor prognosis. We have previously demonstrated that CBX7 is able to inhibit the expression of the SPP1 gene, encoding the chemokine osteopontin that is over-expressed in cancer and has a critical role in cancer progression. Here, we have analyzed the mechanism by which CBX7 regulates the SPP1 gene expression. We show that the SPP1 transcriptional regulation mechanism involves the CBX7-interacting protein HMGA1b, that acts as a positive regulator of the SPP1 gene. In fact, we demonstrate that, in contrast with the transcriptional activity of CBX7, HMGA1b is able to increase the SPP1 expression by inducing the activity of its promoter. Moreover, we show that CBX7 interferes with HMGA1b on the SPP1 promoter and counteracts the positive transcriptional activity of HMGA1b on the SPP1 expression. Furthermore, since we found that also the NF-κB complex resulted involved in the modulation of the SPP1 expression in thyroid cells, we suppose that CBX7/HMGA1b/NF-κB could take part in the same transcriptional mechanism that finally leads to the regulation of the SPP1 gene expression. Taken together, our data show the important role played by CBX7 in the negative regulation of the SPP1 gene expression, thus contributing to prevent the acquisition of a malignant phenotype. PMID:25595895

  3. Hmgn1 acts downstream of C/EBPβ to regulate the decidualization of uterine stromal cells in mice.

    PubMed

    Li, Dang-Dang; Yang, Zhan-Qing; Guo, Chuan-Hui; Yue, Liang; Duan, Cui-Cui; Cao, Hang; Guo, Bin; Yue, Zhan-Peng

    2015-01-01

    Although Hmgn1 is involved in the regulation of gene expression and cellular differentiation, its physiological roles on the differentiation of uterine stromal cells during decidualization still remain unknown. Here we showed that Hmgn1 mRNA was highly expressed in the decidua on days 6-8 of pregnancy. Simultaneously, increased expression of Hmgn1 was also observed in the artificial and in vitro induced decidualization models. Hmgn1 induced the proliferation of uterine stromal cells and expression of Ccna1, Ccnb1, Ccnb2 and Cdk1 in the absence of estrogen and progesterone. Overexpression of Hmgn1 could enhance the expression of Prl8a2 and Prl3c1 which were 2 well-known differentiation markers for decidualization, whereas inhibition of Hmgn1 with specific siRNA could reduce their expression. Further studies found that Hmgn1 could mediate the effects of C/EBPβ on the expression of Prl8a2 and Prl3c1 during in vitro decidualization. In the uterine stromal cells, cAMP analog 8-Br-cAMP could stimulate the expression of Hmgn1 via C/EBPβ. Moreover, siRNA-mediated down-regulation of Hmgn1 could attenuate the effects of cAMP on the differentiation of uterine stromal cells. During in vitro decidualization, Hmgn1 might act downstream of C/EBPβ to regulate the expression of Cox-2, mPGES-1 and Vegf. Progesterone could up-regulate the expression of Hmgn1 in the ovariectomized mouse uterus, uterine epithelial cells and stromal cells. Knockdown of C/EBPβ with siRNA alleviated the up-regulation of progesterone on Hmgn1 expression. Collectively, Hmgn1 may play an important role during mouse decidualization.

  4. Common cis-acting region responsible for transcriptional regulation of Bradyrhizobium japonicum hydrogenase by nickel, oxygen, and hydrogen.

    PubMed Central

    Kim, H; Yu, C; Maier, R J

    1991-01-01

    Bradyrhizobium japonicum expresses hydrogenase in microaerophilic free-living conditions in the presence of nickel. Plasmid-borne hup-lacZ transcriptional fusion constructs were used to study the regulation of the hydrogenase gene. The hydrogenase gene was transcriptionally induced under microaerobic conditions (0.1 to 3.0% partial pressure O2). The hydrogenase gene was not transcribed or was poorly transcribed in strictly anaerobic conditions or conditions above 3.0% O2. Hydrogen gas at levels as low as 0.1% partial pressure induced hydrogenase transcription, and a high level of transcription was maintained up to at least 10% H2 concentration. No transcription was observed in the absence of H2. Hydrogenase was regulated by H2, O2, and Ni when the 5'-upstream sequence was pared down to include base number -168. However, when the upstream sequence was pared down to base number -118, the regulatory response to O2, H2, and Ni levels was negated. Thus, a common cis-acting regulatory region localized within 50 bp is critical for the regulation of hydrogenase by hydrogen, oxygen, and nickel. As a control, the B. japonicum hemA gene which codes for delta-aminolevulinic acid synthase was also fused to the promoterless lacZ gene, and its regulation was tested in the presence of various concentrations of O2 and H2. hemA-lacZ transcription was not dependent on levels of Ni, O2, or H2. Two different hup-lacZ fusions were tested in a Hup- background, strain JH47; these hup-lacZ constructs in JH47 demonstrated dependency on nickel, O2, and H2, indicating that the hydrogenase protein itself is not a sensor for regulation by O2, H2, or nickel. PMID:2061281

  5. Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis

    PubMed Central

    Tian, Caihuan; Wang, Jin; Xu, Tengfei; Xu, Yan; Ohno, Carolyn; Sablowski, Robert; Heisler, Marcus G.; Theres, Klaus; Wang, Ying

    2016-01-01

    Shoot branching requires the establishment of new meristems harboring stem cells; this phenomenon raises questions about the precise regulation of meristematic fate. In seed plants, these new meristems initiate in leaf axils to enable lateral shoot branching. Using live-cell imaging of leaf axil cells, we show that the initiation of axillary meristems requires a meristematic cell population continuously expressing the meristem marker SHOOT MERISTEMLESS (STM). The maintenance of STM expression depends on the leaf axil auxin minimum. Ectopic expression of STM is insufficient to activate axillary buds formation from plants that have lost leaf axil STM expressing cells. This suggests that some cells undergo irreversible commitment to a developmental fate. In more mature leaves, REVOLUTA (REV) directly up-regulates STM expression in leaf axil meristematic cells, but not in differentiated cells, to establish axillary meristems. Cell type-specific binding of REV to the STM region correlates with epigenetic modifications. Our data favor a threshold model for axillary meristem initiation, in which low levels of STM maintain meristematic competence and high levels of STM lead to meristem initiation. PMID:27398935

  6. PP2A and GSK-3beta act antagonistically to regulate active zone development.

    PubMed

    Viquez, Natasha M; Füger, Petra; Valakh, Vera; Daniels, Richard W; Rasse, Tobias M; DiAntonio, Aaron

    2009-09-16

    The synapse is composed of an active zone apposed to a postsynaptic cluster of neurotransmitter receptors. Each Drosophila neuromuscular junction comprises hundreds of such individual release sites apposed to clusters of glutamate receptors. Here, we show that protein phosphatase 2A (PP2A) is required for the development of structurally normal active zones opposite glutamate receptors. When PP2A is inhibited presynaptically, many glutamate receptor clusters are unapposed to Bruchpilot (Brp), an active zone protein required for normal transmitter release. These unapposed receptors are not due to presynaptic retraction of synaptic boutons, since other presynaptic components are still apposed to the entire postsynaptic specialization. Instead, these data suggest that Brp localization is regulated at the level of individual release sites. Live imaging of glutamate receptors demonstrates that this disruption to active zone development is accompanied by abnormal postsynaptic development, with decreased formation of glutamate receptor clusters. Remarkably, inhibition of the serine-threonine kinase GSK-3beta completely suppresses the active zone defect, as well as other synaptic morphology phenotypes associated with inhibition of PP2A. These data suggest that PP2A and GSK-3beta function antagonistically to control active zone development, providing a potential mechanism for regulating synaptic efficacy at a single release site.

  7. Precise regulation of the guidance receptor DMA-1 by KPC-1/Furin instructs dendritic branching decisions

    PubMed Central

    Dong, Xintong; Chiu, Hui; Park, Yeonhee Jenny; Zou, Wei; Zou, Yan; Özkan, Engin; Chang, Chieh; Shen, Kang

    2016-01-01

    Extracellular adhesion molecules and their neuronal receptors guide the growth and branching of axons and dendrites. Growth cones are attracted to intermediate targets, but they must switch their response upon arrival so that they can move away and complete the next stage of growth. Here, we show that KPC-1, a C. elegans Furin homolog, regulates the level of the branching receptor DMA-1 on dendrites by targeting it to late endosomes. In kpc-1 mutants, the level of DMA-1 is abnormally high on dendrites, resulting in trapping of dendrites at locations where a high level of the cognate ligand, the adhesion molecule SAX-7/L1, is present. The misregulation of DMA-1 also causes dendritic self-avoidance defects. Thus, precise regulation of guidance receptors creates flexibility of responses to guidance signals and is critical for neuronal morphogenesis. DOI: http://dx.doi.org/10.7554/eLife.11008.001 PMID:26974341

  8. Precise regulation of the guidance receptor DMA-1 by KPC-1/Furin instructs dendritic branching decisions.

    PubMed

    Dong, Xintong; Chiu, Hui; Park, Yeonhee Jenny; Zou, Wei; Zou, Yan; Özkan, Engin; Chang, Chieh; Shen, Kang

    2016-03-14

    Extracellular adhesion molecules and their neuronal receptors guide the growth and branching of axons and dendrites. Growth cones are attracted to intermediate targets, but they must switch their response upon arrival so that they can move away and complete the next stage of growth. Here, we show that KPC-1, a C. elegans Furin homolog, regulates the level of the branching receptor DMA-1 on dendrites by targeting it to late endosomes. In kpc-1 mutants, the level of DMA-1 is abnormally high on dendrites, resulting in trapping of dendrites at locations where a high level of the cognate ligand, the adhesion molecule SAX-7/L1, is present. The misregulation of DMA-1 also causes dendritic self-avoidance defects. Thus, precise regulation of guidance receptors creates flexibility of responses to guidance signals and is critical for neuronal morphogenesis.

  9. Ubiquitin-Fold Modifier 1 Acts as a Positive Regulator of Breast Cancer

    PubMed Central

    Yoo, Hee Min; Park, Jong Ho; Jeon, Young Joo; Chung, Chin Ha

    2015-01-01

    Estrogen receptor-α (ERα) is a steroid hormone-sensitive transcription factor that plays a critical role in development of breast cancer. The binding of estrogen to ERα triggers the recruitment of transcriptional co-activators as well as chromatin remodeling factors to estrogen-responsive elements (ERE) of ERα target genes. This process is tightly associated with post-translational modifications (PTMs) of ERα and its co-activators for promotion of transcriptional activation, which leads to proliferation of a large subset of breast tumor cells. These PTMs include phosphorylation, acetylation, methylation, and conjugation by ubiquitin and ubiquitin-like proteins. Ubiquitin-fold modifier 1 (UFM1), one of ubiquitin-like proteins, has recently been shown to be ligated to activating signal co-integrator 1 (ASC1), which acts as a transcriptional co-activator of nuclear receptors. Here, we discuss the mechanistic connection between ASC1 modification by UFM1 and ERα transactivation, and highlight how the interplay of these processes is involved in development of breast cancer. We also discuss potential use of UFM1-conjugating system as therapeutic targets against not only breast cancer but also other nuclear receptor-mediated cancers. PMID:25852645

  10. 28 CFR Appendix D to Subpart G of... - OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 1 2012-07-01 2012-07-01 false OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) D..., App. D Appendix D to Subpart G of Part 42—OJARS' Regulations Under the Omnibus Crime Control and...

  11. 28 CFR Appendix D to Subpart G of... - OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 1 2013-07-01 2013-07-01 false OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) D..., App. D Appendix D to Subpart G of Part 42—OJARS' Regulations Under the Omnibus Crime Control and...

  12. 28 CFR Appendix D to Subpart G of... - OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 1 2011-07-01 2011-07-01 false OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) D..., App. D Appendix D to Subpart G of Part 42—OJARS' Regulations Under the Omnibus Crime Control and...

  13. 28 CFR Appendix D to Subpart G of... - OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 1 2014-07-01 2014-07-01 false OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) D..., App. D Appendix D to Subpart G of Part 42—OJARS' Regulations Under the Omnibus Crime Control and...

  14. Humic acid acts as a natural antidote of graphene by regulating nanomaterial translocation and metabolic fluxes in vivo.

    PubMed

    Hu, Xiangang; Mu, Li; Kang, Jia; Lu, Kaicheng; Zhou, Ruiren; Zhou, Qixing

    2014-06-17

    Graphene-related research has intensified rapidly in a wide range of disciplines, but few studies have examined ecosystem risks, particularly phytotoxicity. This study revealed that graphene significantly inhibits the number of wheat roots and the biosynthesis of chlorophyll, and altered the morphology of shoots. Humic acid (HA), a ubiquitous form of natural organic matter, significantly (P < 0.05) relieved this phytotoxicity and recovered the sharp morphology of shoot tips. Both graphene and graphene-HA were transferred from wheat roots to shoots and were found in the cytoplasms and chloroplasts. HA increased the disordered structure and surface negative charges, and reduced the aggregation of graphene. HA enhanced the storage of graphene in vacuoles, potentially indicating an effective detoxification path. The content of cadaverine, alkane, glyconic acid, and aconitic acid was up-regulated by graphene, greatly contributing to the observed phytotoxicity. Conversely, inositol, phenylalanine, phthalic acid, and octadecanoic acid were up-regulated by graphene-HA. The metabolic pathway analysis revealed that the direction of metabolic fluxes governed nanotoxicity. This work presents the innovative concept that HA acts as a natural antidote of graphene by regulating its translocation and metabolic fluxes in vivo. This knowledge is critical for avoiding the overestimation of nanomaterial risks and can be used to control nanomaterial contamination.

  15. Regulated expression of mammalian histone H4 genes in vivo requires a trans-acting transcription factor.

    PubMed Central

    Capasso, O; Heintz, N

    1985-01-01

    Mouse L cells containing integrated copies of a human histone H4 gene have been obtained by cotransfection with the herpesvirus thymidine kinase gene. Nuclease S1 assays of RNA from several independent cell lines show that the expression of the introduced H4 gene is regulated during the cell cycle. One of these cell lines (line 6-8) contains more than 60 human H4 gene copies per haploid genome and does not express the endogenous mouse histone H4 mRNA. In contrast, the expression of the mouse H2a and H3 mRNAs in this cell line is not perturbed. In cell revertants that have lost the majority of the human H4 gene copies, the expression of the mouse H4 mRNA is restored, demonstrating that the mouse genes remain functional although not expressed. The rate of transcription of the histone H4 genes in clone 6-8 is at least 10-fold greater than that of the parental cell line and it is regulated during traversal of the cell cycle. These results show that the expression of mammalian histone H4 genes involves both a trans-acting transcriptional regulatory factor and an H4-specific activity. We propose that cell cycle regulation of histone gene expression may be effected through subtype-specific transcriptional regulatory proteins. Images PMID:3862085

  16. Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission

    PubMed Central

    Ghosh, Himanish; Auguadri, Luca; Battaglia, Sereina; Simone Thirouin, Zahra; Zemoura, Khaled; Messner, Simon; Acuña, Mario A.; Wildner, Hendrik; Yévenes, Gonzalo E.; Dieter, Andrea; Kawasaki, Hiroshi; O. Hottiger, Michael; Zeilhofer, Hanns Ulrich; Fritschy, Jean-Marc; Tyagarajan, Shiva K.

    2016-01-01

    GABAA receptors (GABAARs) mediate the majority of fast inhibitory neurotransmission in the brain via synergistic association with the postsynaptic scaffolding protein gephyrin and its interaction partners. However, unlike their counterparts at glutamatergic synapses, gephyrin and its binding partners lack canonical protein interaction motifs; hence, the molecular basis for gephyrin scaffolding has remained unclear. In this study, we identify and characterize two new posttranslational modifications of gephyrin, SUMOylation and acetylation. We demonstrate that crosstalk between SUMOylation, acetylation and phosphorylation pathways regulates gephyrin scaffolding. Pharmacological intervention of SUMO pathway or transgenic expression of SUMOylation-deficient gephyrin variants rescued gephyrin clustering in CA1 or neocortical neurons of Gabra2-null mice, which otherwise lack gephyrin clusters, indicating that gephyrin SUMO modification is an essential determinant for scaffolding at GABAergic synapses. Together, our results demonstrate that concerted modifications on a protein scaffold by evolutionarily conserved yet functionally diverse signalling pathways facilitate GABAergic transmission. PMID:27819299

  17. Methamphetamine acts on subpopulations of neurons regulating sexual behavior in male rats

    PubMed Central

    Frohmader, Karla S.; Wiskerke, Joost; Wise, Roy A.; Lehman, Michael N.; Coolen, Lique M.

    2010-01-01

    Methamphetamine (Meth) is a highly addictive stimulant. Meth abuse is commonly associated with the practice of sexual risk behavior and increased prevalence of Human Immunodeficiency Virus and Meth users report heightened sexual desire, arousal, and sexual pleasure. The biological basis for this drug-sex nexus is unknown. The current study demonstrates that Meth administration in male rats activates neurons in brain regions of the mesolimbic system that are involved in the regulation of sexual behavior. Specifically, Meth and mating co-activate cells in the nucleus accumbens core and shell, basolateral amygdala, and anterior cingulate cortex. These findings illustrate that in contrast to current belief drugs of abuse can activate the same cells as a natural reinforcer, i.e. sexual behavior, and in turn may influence compulsive seeking of this natural reward. PMID:20045448

  18. Gene regulation by the act of long non-coding RNA transcription

    PubMed Central

    2013-01-01

    Long non-protein-coding RNAs (lncRNAs) are proposed to be the largest transcript class in the mouse and human transcriptomes. Two important questions are whether all lncRNAs are functional and how they could exert a function. Several lncRNAs have been shown to function through their product, but this is not the only possible mode of action. In this review we focus on a role for the process of lncRNA transcription, independent of the lncRNA product, in regulating protein-coding-gene activity in cis. We discuss examples where lncRNA transcription leads to gene silencing or activation, and describe strategies to determine if the lncRNA product or its transcription causes the regulatory effect. PMID:23721193

  19. The glucocorticoid receptor is a key regulator of the decision between self-renewal and differentiation in erythroid progenitors.

    PubMed Central

    Wessely, O; Deiner, E M; Beug, H; von Lindern, M

    1997-01-01

    During development and in regenerating tissues such as the bone marrow, progenitor cells constantly need to make decisions between proliferation and differentiation. We have used a model system, normal erythroid progenitors of the chicken, to determine the molecular players involved in making this decision. The molecules identified comprised receptor tyrosine kinases (c-Kit and c-ErbB) and members of the nuclear hormone receptor superfamily (thyroid hormone receptor and estrogen receptor). Here we identify the glucocorticoid receptor (GR) as a key regulator of erythroid progenitor self-renewal (i.e. continuous proliferation in the absence of differentiation). In media lacking a GR ligand or containing a GR antagonist, erythroid progenitors failed to self-renew, even if c-Kit, c-ErbB and the estrogen receptor were activated simultaneously. To induce self-renewal, the GR required the continuous presence of an activated receptor tyrosine kinase and had to cooperate with the estrogen receptor for full activity. Mutant analysis showed that DNA binding and a functional AF-2 transactivation domain are required for proliferation stimulation and differentiation arrest. c-myb was identified as a potential target gene of the GR in erythroblasts. It could be demonstrated that delta c-Myb, an activated c-Myb protein, can functionally replace the GR. PMID:9029148

  20. Phosphorylation of the Synaptonemal Complex Protein Zip1 Regulates the Crossover/Noncrossover Decision during Yeast Meiosis.

    PubMed

    Chen, Xiangyu; Suhandynata, Ray T; Sandhu, Rima; Rockmill, Beth; Mohibullah, Neeman; Niu, Hengyao; Liang, Jason; Lo, Hsiao-Chi; Miller, Danny E; Zhou, Huilin; Börner, G Valentin; Hollingsworth, Nancy M

    2015-12-01

    Interhomolog crossovers promote proper chromosome segregation during meiosis and are formed by the regulated repair of programmed double-strand breaks. This regulation requires components of the synaptonemal complex (SC), a proteinaceous structure formed between homologous chromosomes. In yeast, SC formation requires the "ZMM" genes, which encode a functionally diverse set of proteins, including the transverse filament protein, Zip1. In wild-type meiosis, Zmm proteins promote the biased resolution of recombination intermediates into crossovers that are distributed throughout the genome by interference. In contrast, noncrossovers are formed primarily through synthesis-dependent strand annealing mediated by the Sgs1 helicase. This work identifies a conserved region on the C terminus of Zip1 (called Zip1 4S), whose phosphorylation is required for the ZMM pathway of crossover formation. Zip1 4S phosphorylation is promoted both by double-strand breaks (DSBs) and the meiosis-specific kinase, MEK1/MRE4, demonstrating a role for MEK1 in the regulation of interhomolog crossover formation, as well as interhomolog bias. Failure to phosphorylate Zip1 4S results in meiotic prophase arrest, specifically in the absence of SGS1. This gain of function meiotic arrest phenotype is suppressed by spo11Δ, suggesting that it is due to unrepaired breaks triggering the meiotic recombination checkpoint. Epistasis experiments combining deletions of individual ZMM genes with sgs1-md zip1-4A indicate that Zip1 4S phosphorylation functions prior to the other ZMMs. These results suggest that phosphorylation of Zip1 at DSBs commits those breaks to repair via the ZMM pathway and provides a mechanism by which the crossover/noncrossover decision can be dynamically regulated during yeast meiosis.

  1. Phosphorylation of the Synaptonemal Complex Protein Zip1 Regulates the Crossover/Noncrossover Decision during Yeast Meiosis

    PubMed Central

    Chen, Xiangyu; Suhandynata, Ray T.; Sandhu, Rima; Rockmill, Beth; Mohibullah, Neeman; Niu, Hengyao; Liang, Jason; Lo, Hsiao-Chi; Miller, Danny E.; Zhou, Huilin; Börner, G. Valentin; Hollingsworth, Nancy M.

    2015-01-01

    Interhomolog crossovers promote proper chromosome segregation during meiosis and are formed by the regulated repair of programmed double-strand breaks. This regulation requires components of the synaptonemal complex (SC), a proteinaceous structure formed between homologous chromosomes. In yeast, SC formation requires the “ZMM” genes, which encode a functionally diverse set of proteins, including the transverse filament protein, Zip1. In wild-type meiosis, Zmm proteins promote the biased resolution of recombination intermediates into crossovers that are distributed throughout the genome by interference. In contrast, noncrossovers are formed primarily through synthesis-dependent strand annealing mediated by the Sgs1 helicase. This work identifies a conserved region on the C terminus of Zip1 (called Zip1 4S), whose phosphorylation is required for the ZMM pathway of crossover formation. Zip1 4S phosphorylation is promoted both by double-strand breaks (DSBs) and the meiosis-specific kinase, MEK1/MRE4, demonstrating a role for MEK1 in the regulation of interhomolog crossover formation, as well as interhomolog bias. Failure to phosphorylate Zip1 4S results in meiotic prophase arrest, specifically in the absence of SGS1. This gain of function meiotic arrest phenotype is suppressed by spo11Δ, suggesting that it is due to unrepaired breaks triggering the meiotic recombination checkpoint. Epistasis experiments combining deletions of individual ZMM genes with sgs1-md zip1-4A indicate that Zip1 4S phosphorylation functions prior to the other ZMMs. These results suggest that phosphorylation of Zip1 at DSBs commits those breaks to repair via the ZMM pathway and provides a mechanism by which the crossover/noncrossover decision can be dynamically regulated during yeast meiosis. PMID:26682552

  2. Hemoglobin Control of Cell Survival/Death Decision Regulates in Vitro Plant Embryogenesis1[W][OPEN

    PubMed Central

    Huang, Shuanglong; Hill, Robert D.; Wally, Owen S.D.; Dionisio, Giuseppe; Ayele, Belay T.; Jami, Sravan Kumar; Stasolla, Claudio

    2014-01-01

    Programmed cell death (PCD) in multicellular organisms is a vital process in growth, development, and stress responses that contributes to the formation of tissues and organs. Although numerous studies have defined the molecular participants in apoptotic and PCD cascades, successful identification of early master regulators that target specific cells to live or die is limited. Using Zea mays somatic embryogenesis as a model system, we report that the expressions of two plant hemoglobin (Hb) genes (ZmHb1 and ZmHb2) regulate the cell survival/death decision that influences somatic embryogenesis through their cell-specific localization patterns. Suppression of either of the two ZmHbs is sufficient to induce PCD through a pathway initiated by elevated NO and Zn2+ levels and mediated by production of reactive oxygen species. The effect of the death program on the fate of the developing embryos is dependent on the localization patterns of the two ZmHbs. During somatic embryogenesis, ZmHb2 transcripts are restricted to a few cells anchoring the embryos to the subtending embryogenic tissue, whereas ZmHb1 transcripts extend to several embryonic domains. Suppression of ZmHb2 induces PCD in the anchoring cells, allowing the embryos to develop further, whereas suppression of ZmHb1 results in massive PCD, leading to abortion. We conclude that regulation of the expression of these ZmHbs has the capability to determine the developmental fate of the embryogenic tissue during somatic embryogenesis through their effect on PCD. This unique regulation might have implications for development and differentiation in other species. PMID:24784758

  3. Cis-Acting Polymorphisms Affect Complex Traits through Modifications of MicroRNA Regulation Pathways

    PubMed Central

    Hartsperger, Mara L.; Pfeufer, Arne; Stümpflen, Volker

    2012-01-01

    Genome-wide association studies (GWAS) have become an effective tool to map genes and regions contributing to multifactorial human diseases and traits. A comparably small number of variants identified by GWAS are known to have a direct effect on protein structure whereas the majority of variants is thought to exert their moderate influences on the phenotype through regulatory changes in mRNA expression. MicroRNAs (miRNAs) have been identified as powerful posttranscriptional regulators of mRNAs. Binding to their target sites, which are mostly located within the 3′-untranslated region (3′-UTR) of mRNA transcripts, they modulate mRNA expression and stability. Until today almost all human mRNA transcripts are known to harbor at least one miRNA target site with an average of over 20 miRNA target sites per transcript. Among 5,101 GWAS-identified sentinel single nucleotide polymorphisms (SNPs) that correspond to 18,884 SNPs in linkage disequilibrium (LD) with the sentinels () we identified a significant overrepresentation of SNPs that affect the 3′-UTR of genes (OR = 2.33, 95% CI = 2.12–2.57, ). This effect was even stronger considering all SNPs in one LD bin a single signal (OR = 4.27, 95% CI = 3.84–4.74, ). Based on crosslinking immunoprecipitation data we identified four mechanisms affecting miRNA regulation by 3′-UTR mutations: (i) deletion or (ii) creation of miRNA recognition elements within validated RNA-induced silencing complex binding sites, (iii) alteration of 3′-UTR splicing leading to a loss of binding sites, and (iv) change of binding affinity due to modifications of 3′-UTR folding. We annotated 53 SNPs of a total of 288 trait-associated 3′-UTR SNPs as mediating at least one of these mechanisms. Using a qualitative systems biology approach, we demonstrate how our findings can be used to support biological interpretation of GWAS results as well as to provide new experimentally testable hypotheses. PMID:22606281

  4. A wheat homolog of MOTHER OF FT AND TFL1 acts in the regulation of germination.

    PubMed

    Nakamura, Shingo; Abe, Fumitaka; Kawahigashi, Hiroyuki; Nakazono, Kou; Tagiri, Akemi; Matsumoto, Takashi; Utsugi, Shigeko; Ogawa, Taiichi; Handa, Hirokazu; Ishida, Hiroki; Mori, Masahiko; Kawaura, Kanako; Ogihara, Yasunari; Miura, Hideho

    2011-09-01

    Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs.ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this increased expression correlated with a single nucleotide polymorphism in the promoter region. In a complementation analysis, high levels of MFT expression were correlated with a low germination index in T1 seeds. Furthermore, precocious germination of isolated immature embryos was suppressed by transient introduction of MFT driven by the maize (Zea mays) ubiquitin promoter. Taken together, these results suggest that MFT plays an important role in the regulation of germination in wheat.

  5. Vinculin acts as a sensor in lipid regulation of adhesion-site turnover.

    PubMed

    Chandrasekar, Indra; Stradal, Theresia E B; Holt, Mark R; Entschladen, Frank; Jockusch, Brigitte M; Ziegler, Wolfgang H

    2005-04-01

    The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of this interaction are poorly understood. To study the significance of phospholipid binding to vinculin for adhesion-site turnover and cell motility, we constructed a mutant, vinculin-LD, deficient in acidic phospholipid binding yet with functional actin-binding sites. When expressed in cells, vinculin-LD was readily recruited to adhesion sites, as judged by fluorescence recovery after photobleaching (FRAP) analysis, but cell spreading and migration were strongly impaired, and PIP(2)-dependent disassembly of adhesions was suppressed. Thus, PIP(2) binding is not essential for vinculin activation and recruitment, as previously suggested. Instead, we propose that PIP(2) levels can regulate the uncoupling of adhesion sites from the actin cytoskeleton, with vinculin functioning as a sensor.

  6. Synemin acts as a regulator of signalling molecules during skeletal muscle hypertrophy.

    PubMed

    Li, Zhenlin; Parlakian, Ara; Coletti, Dario; Alonso-Martin, Sonia; Hourdé, Christophe; Joanne, Pierre; Gao-Li, Jacqueline; Blanc, Jocelyne; Ferry, Arnaud; Paulin, Denise; Xue, Zhigang; Agbulut, Onnik

    2014-11-01

    Synemin, a type IV intermediate filament (IF) protein, forms a bridge between IFs and cellular membranes. As an A-kinase-anchoring protein, it also provides temporal and spatial targeting of protein kinase A (PKA). However, little is known about its functional roles in either process. To better understand its functions in muscle tissue, we generated synemin-deficient (Synm(-) (/-)) mice. Synm(-) (/-) mice displayed normal development and fertility but showed a mild degeneration and regeneration phenotype in myofibres and defects in sarcolemma membranes. Following mechanical overload, Synm(-) (/-) mice muscles showed a higher hypertrophic capacity with increased maximal force and fatigue resistance compared with control mice. At the molecular level, increased remodelling capacity was accompanied by decreased myostatin (also known as GDF8) and atrogin (also known as FBXO32) expression, and increased follistatin expression. Furthermore, the activity of muscle-mass control molecules (the PKA RIIα subunit, p70S6K and CREB1) was increased in mutant mice. Finally, analysis of muscle satellite cell behaviour suggested that the absence of synemin could affect the balance between self-renewal and differentiation of these cells. Taken together, our results show that synemin is necessary to maintain membrane integrity and regulates signalling molecules during muscle hypertrophy.

  7. Two small GTPases act in concert with the bactofilin cytoskeleton to regulate dynamic bacterial cell polarity.

    PubMed

    Bulyha, Iryna; Lindow, Steffi; Lin, Lin; Bolte, Kathrin; Wuichet, Kristin; Kahnt, Jörg; van der Does, Chris; Thanbichler, Martin; Søgaard-Andersen, Lotte

    2013-04-29

    Cell polarity is essential for many bacterial activities, but the mechanisms responsible for its establishment are poorly understood. In Myxococcus xanthus, the type IV pili (T4P) motor ATPases PilB and PilT localize to opposite cell poles and switch poles during cellular reversals. We demonstrate that polar localization of PilB and PilT depends on the small GTPase SofG and BacP, a bactofilin cytoskeletal protein. Polymeric BacP localizes in both subpolar regions. SofG interacts directly with polymeric BacP and associates with one of these patches, forming a cluster that shuttles to the pole to establish localization of PilB and PilT at the same pole. Next, the small GTPase MglA sorts PilB and PilT to opposite poles to establish their correct polarity. During reversals, the Frz chemosensory system induces the inversion of PilB and PilT polarity. Thus, three hierarchically organized systems function in a cascade to regulate dynamic bacterial cell polarity.

  8. cis- and trans-acting elements of the estrogen-regulated vitellogenin gene B1 of Xenopus laevis.

    PubMed

    Wahli, W; Martinez, E; Corthésy, B; Cardinaux, J R

    1989-01-01

    Vitellogenin genes are expressed under strict estrogen control in the liver of female oviparous vertebrates. Gene transfer experiments using estrogen-responsive cells have shown that the 13 bp perfect palindromic element GGTCACTGTGACC found upstream of the Xenopus laevis vitellogenin gene A2 promoter mediates hormonal stimulation and thus, was called the estrogen-responsive element (ERE). In the Xenopus vitellogenin genes B1 and B2 there are two closely adjacent EREs with one or more base substitutions when compared to the consensus ERE GGTCANNNTGACC. On their own, these degenerated elements have only a low or no regulatory capacity at all but act together synergistically to form an estrogen-responsive unit (ERU) with the same strength as the perfect palindromic 13 bp element. Analysis of estrogen receptor binding to the gene B1 ERU revealed a cooperative interaction of receptor dimers to the two adjacent imperfect EREs which most likely explains the synergistic stimulation observed in vivo. Furthermore, a promoter activator element located between positions --113 and --42 of the gene B1 and functional in the human MCF-7 and the Xenopus B3.2 cells has been identified and shown to be involved in the high level of induced transcription activity when the ERE is placed at a distance from the promoter. Finally, a hormone-controlled in vitro transcription system derived from Xenopus liver nuclear extracts was exploited to characterize two additional novel cis-acting elements within the vitellogenin gene B1 promoter. One of them, a negative regulatory element (NRE), is responsible for repression of promoter activity in the absence of hormone. The second is related to the NF-I binding site and is required, together with the ERE, to mediate hormonal induction. Moreover, we detected three trans-acting activities in Xenopus liver nuclear extracts that interact with these regions and demonstrated that they participate in the regulation of the expression of the vitellogenin

  9. Immunomodulatory drugs act as inhibitors of DNA methyltransferases and induce PU.1 up-regulation in myeloma cells.

    PubMed

    Endo, Shinya; Amano, Masayuki; Nishimura, Nao; Ueno, Niina; Ueno, Shikiko; Yuki, Hiromichi; Fujiwara, Shiho; Wada, Naoko; Hirata, Shinya; Hata, Hiroyuki; Mitsuya, Hiroaki; Okuno, Yutaka

    2016-01-08

    Immunomodulatory drugs (IMiDs) such as thalidomide, lenalidomide, and pomalidomide are efficacious in the treatment of multiple myeloma and significantly prolong their survival. However, the mechanisms of such effects of IMiDs have not been fully elucidated. Recently, cereblon has been identified as a target binding protein of thalidomide. Lenalidomide-resistant myeloma cell lines often lose the expression of cereblon, suggesting that IMiDs act as an anti-myeloma agent through interacting with cereblon. Cereblon binds to damaged DNA-binding protein and functions as a ubiquitin ligase, inducing degradation of IKZF1 and IKZF3 that are essential transcription factors for B and T cell development. Degradation of both IKZF1 and IKZF3 reportedly suppresses myeloma cell growth. Here, we found that IMiDs act as inhibitors of DNA methyltransferases (DMNTs). We previously reported that PU.1, which is an ETS family transcription factor and essential for myeloid and lymphoid development, functions as a tumor suppressor in myeloma cells. PU.1 induces growth arrest and apoptosis of myeloma cell lines. In this study, we found that low-dose lenalidomide and pomalidomide up-regulate PU.1 expression through inducing demethylation of the PU.1 promoter. In addition, IMiDs inhibited DNMT1, DNMT3a, and DNMT3b activities in vitro. Furthermore, lenalidomide and pomalidomide decreased the methylation status of the whole genome in myeloma cells. Collectively, IMiDs exert demethylation activity through inhibiting DNMT1, 3a, and 3b, and up-regulating PU.1 expression, which may be one of the mechanisms of the anti-myeloma activity of IMiDs.

  10. The Exosome and Trans-Acting Small Interfering RNAs Regulate Cuticular Wax Biosynthesis during Arabidopsis Inflorescence Stem Development1[OPEN

    PubMed Central

    Lam, Patricia; Zhao, Lifang; Eveleigh, Nathan; Yu, Yu; Chen, Xuemei

    2015-01-01

    The primary aerial surfaces of land plants are covered with a cuticle, a protective layer composed of the cutin polyester matrix and cuticular waxes. Previously, we discovered a unique mechanism of regulating cuticular wax biosynthesis during Arabidopsis (Arabidopsis thaliana) stem elongation that involves ECERIFERUM7 (CER7), a core subunit of the exosome. Because loss-of-function mutations in CER7 result in reduced expression of the wax biosynthetic gene CER3, we proposed that CER7 is involved in degrading a messenger RNA encoding a CER3 repressor. To identify this putative repressor, we performed a cer7 suppressor screen that resulted in the isolation of the posttranscriptional gene-silencing components RNA-DEPENDENT RNA POLYMERASE1 and SUPPRESSOR OF GENE SILENCING3, indicating that small RNAs regulate CER3 expression. To establish the identity of the effector RNA species and determine whether these RNAs control CER3 transcript levels directly, we cloned additional genes identified in our suppressor screen and performed next-generation sequencing of small RNA populations that differentially accumulate in the cer7 mutant in comparison with the wild type. Our results demonstrate that the trans-acting small interfering RNA class of small RNAs are the effector molecules involved in direct silencing of CER3 and that the expression of five additional genes (EARLY RESPONSE TO DEHYDRATION14, AUXIN RESISTANT1, a translation initiation factor SUI1 family protein, and two genes of unknown function) is controlled by both CER7 and trans-acting small interfering RNAs. PMID:25502190

  11. Sequoia regulates cell fate decisions in the external sensory organs of adult Drosophila.

    PubMed

    Andrews, Hillary K; Giagtzoglou, Nikolaos; Yamamoto, Shinya; Schulze, Karen L; Bellen, Hugo J

    2009-06-01

    The adult Drosophila external sensory organ (ESO), comprising the hair, socket, neuron, sheath and glia cells, arises through the asymmetric division of sensory organ precursor cells (SOPs). In a mosaic screen designed to identify new components in ESO development, we isolated mutations in sequoia, which encodes a putative zinc-finger transcription factor that has previously been shown to have a role in dendritogenesis. Here, we show that adult clones mutant for seq exhibit a loss of hair cells and a gain of socket cells. We propose that the seq mutant phenotype arises, in part, owing to the loss of several crucial transcription factors known to be important in peripheral nervous system development such as D-Pax2, Prospero and Hamlet. Thus, Sequoia is a new upstream regulator of genes that orchestrates cell fate specification during development of the adult ESO lineage.

  12. SHP-2 acts via ROCK to regulate the cardiac actin cytoskeleton.

    PubMed

    Langdon, Yvette; Tandon, Panna; Paden, Erika; Duddy, Jennifer; Taylor, Joan M; Conlon, Frank L

    2012-03-01

    Noonan syndrome is one of the most common causes of human congenital heart disease and is frequently associated with missense mutations in the protein phosphatase SHP-2. Interestingly, patients with acute myelogenous leukemia (AML), acute lymphoblastic leukemia (ALL), juvenile myelomonocytic leukemia (JMML) and LEOPARD syndrome frequently carry a second, somatically introduced subset of missense mutations in SHP-2. To determine the cellular and molecular mechanisms by which SHP-2 regulates heart development and, thus, understand how Noonan-associated mutations affect cardiogenesis, we introduced SHP-2 encoding the most prevalent Noonan syndrome and JMML mutations into Xenopus embryos. Resulting embryos show a direct relationship between a Noonan SHP-2 mutation and its ability to cause cardiac defects in Xenopus; embryos expressing Noonan SHP-2 mutations exhibit morphologically abnormal hearts, whereas those expressing an SHP-2 JMML-associated mutation do not. Our studies indicate that the cardiac defects associated with the introduction of the Noonan-associated SHP-2 mutations are coupled with a delay or arrest of the cardiac cell cycle in M-phase and a failure of cardiomyocyte progenitors to incorporate into the developing heart. We show that these defects are a result of an underlying malformation in the formation and polarity of cardiac actin fibers and F-actin deposition. We show that these defects can be rescued in culture and in embryos through the inhibition of the Rho-associated, coiled-coil-containing protein kinase 1 (ROCK), thus demonstrating a direct relationship between SHP-2(N308D) and ROCK activation in the developing heart.

  13. Transcription factor FBI-1 acts as a dual regulator in adipogenesis by coordinated regulation of cyclin-A and E2F-4.

    PubMed

    Laudes, Matthias; Bilkovski, Roman; Oberhauser, Frank; Droste, Andrea; Gomolka, Matthias; Leeser, Uschi; Udelhoven, Michael; Krone, Wilhelm

    2008-05-01

    Generation of new adipocytes plays a major role in the development of obesity. We previously have shown that transcriptional repressor factor that binds to IST (FBI)-1 exerts a dual effect in the process of adipogenesis by inhibiting proliferation and promoting differentiation of preadipocytes. The aim of the present study was to identify FBI-1 regulated molecular effectors that could account for these effects. Overexpressing FBI-1 in preadipocytes resulted in reduced expression of the cell cycle regulator cyclin A, which may explain FBI-1 induced inhibition of proliferation. Interestingly, FBI-1 repressed cyclin A promoter activity through an indirect mechanisms that did not involve direct binding of FBI-1 to the promoter sequence, but rather FBI-1 inhibition of transcriptional activator Sp1 binding to a regulatory element at -452 to -443. We also show that FBI-1 promotes terminal preadipocyte differentiation through a mechanism involving decreased levels of expression of the PPARgamma inhibitor E2F-4. FBI-1 significantly reduced E2F-4 promoter activity. Contrary to cyclin A, we found FBI-1-induced repression of E2F-4 is mediated by a direct mechanism via a FBI-1 regulatory element at -11 to -5. As function of transcriptional repressors normally depends on the presence of regulatory co-factors we also performed expression profiling of potential FBI-1 co-repressors throughout adipogenesis. In these experiments Sin3A and histon deacetylase (HDAC)-1 showed a similar expression pattern compared to FBI-1. Strikingly, co-immunoprecipitation studies revealed that FBI-1 binds Sin3A and HDAC-1 to form a repressor complex. Furthermore, by mutational analysis the amino terminal Poxvirus (POZ) domain of FBI-1 was found to be important for Sin3A and HDAC-1 binding. Taken together, FBI-1 is the first transcriptional repressor shown to act as a dual regulator in adipogenesis exerting repressor activities on target genes by both, direct and indirect mechanisms.

  14. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis[C][W][OPEN

    PubMed Central

    Xu, Jie; Ding, Zhiwen; Vizcay-Barrena, Gema; Shi, Jianxin; Liang, Wanqi; Yuan, Zheng; Werck-Reichhart, Danièle; Schreiber, Lukas; Wilson, Zoe A.; Zhang, Dabing

    2014-01-01

    Mature pollen is covered by durable cell walls, principally composed of sporopollenin, an evolutionary conserved, highly resilient, but not fully characterized, biopolymer of aliphatic and aromatic components. Here, we report that ABORTED MICROSPORES (AMS) acts as a master regulator coordinating pollen wall development and sporopollenin biosynthesis in Arabidopsis thaliana. Genome-wide coexpression analysis revealed 98 candidate genes with specific expression in the anther and 70 that showed reduced expression in ams. Among these 70 members, we showed that AMS can directly regulate 23 genes implicated in callose dissociation, fatty acids elongation, formation of phenolic compounds, and lipidic transport putatively involved in sporopollenin precursor synthesis. Consistently, ams mutants showed defective microspore release, a lack of sporopollenin deposition, and a dramatic reduction in total phenolic compounds and cutin monomers. The functional importance of the AMS pathway was further demonstrated by the observation of impaired pollen wall architecture in plant lines with reduced expression of several AMS targets: the abundant pollen coat protein extracellular lipases (EXL5 and EXL6), and CYP98A8 and CYP98A9, which are enzymes required for the production of phenolic precursors. These findings demonstrate the central role of AMS in coordinating sporopollenin biosynthesis and the secretion of materials for pollen wall patterning. PMID:24781116

  15. CK2 acts as a potent negative regulator of receptor-mediated insulin release in vitro and in vivo

    PubMed Central

    Rossi, Mario; Ruiz de Azua, Inigo; Barella, Luiz F.; Sakamoto, Wataru; Zhu, Lu; Cui, Yinghong; Lu, Huiyan; Rebholz, Heike; Matschinsky, Franz M.; Doliba, Nicolai M.; Butcher, Adrian J.; Tobin, Andrew B.; Wess, Jürgen

    2015-01-01

    G protein-coupled receptors (GPCRs) regulate virtually all physiological functions including the release of insulin from pancreatic β-cells. β-Cell M3 muscarinic receptors (M3Rs) are known to play an essential role in facilitating insulin release and maintaining proper whole-body glucose homeostasis. As is the case with other GPCRs, M3R activity is regulated by phosphorylation by various kinases, including GPCR kinases and casein kinase 2 (CK2). At present, it remains unknown which of these various kinases are physiologically relevant for the regulation of β-cell activity. In the present study, we demonstrate that inhibition of CK2 in pancreatic β-cells, knockdown of CK2α expression, or genetic deletion of CK2α in β-cells of mutant mice selectively augmented M3R-stimulated insulin release in vitro and in vivo. In vitro studies showed that this effect was associated with an M3R-mediated increase in intracellular calcium levels. Treatment of mouse pancreatic islets with CX4945, a highly selective CK2 inhibitor, greatly reduced agonist-induced phosphorylation of β-cell M3Rs, indicative of CK2-mediated M3R phosphorylation. We also showed that inhibition of CK2 greatly enhanced M3R-stimulated insulin secretion in human islets. Finally, CX4945 treatment protected mice against diet-induced hyperglycemia and glucose intolerance in an M3R-dependent fashion. Our data demonstrate, for the first time to our knowledge, the physiological relevance of CK2 phosphorylation of a GPCR and suggest the novel concept that kinases acting on β-cell GPCRs may represent novel therapeutic targets. PMID:26598688

  16. Fbxw7 regulates lipid metabolism and cell fate decisions in the mouse liver

    PubMed Central

    Onoyama, Ichiro; Suzuki, Atsushi; Matsumoto, Akinobu; Tomita, Kengo; Katagiri, Hideki; Oike, Yuichi; Nakayama, Keiko; Nakayama, Keiichi I.

    2010-01-01

    E3 ubiquitin ligase complexes of the SCF type consist of ring-box 1 (Rbx1), cullin 1 (Cul1), S-phase kinase-associated protein 1 (Skp1), and a member of the F-box family of proteins. The identity of the F-box protein determines the substrate specificity of the complex. The F-box family member F-box– and WD repeat domain–containing 7 (Fbxw7; also known as Fbw7, SEL-10, hCdc4, and hAgo) targets for degradation proteins with wide-ranging functions, and uncovering its in vivo role has been difficult, because Fbxw7–/– embryos die in utero. Using two different Cre-loxP systems (Mx1-Cre and Alb-Cre), we generated mice with liver-specific null mutations of Fbxw7. Hepatic ablation of Fbxw7 resulted in hepatomegaly and steatohepatitis, with massive deposition of triglyceride, a phenotype similar to that observed in humans with nonalcoholic steatohepatitis. Both cell proliferation and the abundance of Fbxw7 substrates were increased in the Fbxw7-deficient liver. Long-term Fbxw7 deficiency resulted in marked proliferation of the biliary system and the development of hamartomas. Fbxw7 deficiency also skewed the differentiation of liver stem cells toward the cholangiocyte lineage rather than the hepatocyte lineage in vitro. This bias was corrected by additional loss of the Notch cofactor RBP-J, suggesting that Notch accumulation triggered the abnormal proliferation of the biliary system. Together, our results suggest that Fbxw7 plays key roles, regulating lipogenesis and cell proliferation and differentiation in the liver. PMID:21123947

  17. Recovery Act: Head Start Grantees Expand Services, but More Consistent Communication Could Improve Accountability and Decisions about Spending. Report to the Congress. GAO-11-166

    ERIC Educational Resources Information Center

    Ashby, Cornelia M.; Jones, Yvonne D.

    2010-01-01

    This report responds to two mandates for GAO under the American Recovery and Reinvestment Act of 2009 (Recovery Act). First, it is the latest report on the uses of and accountability for Recovery Act funds in selected states and localities. Second, it comments on recipients' reports of the jobs created and retained. The Recovery Act provided $2.1…

  18. miR-600 Acts as a Bimodal Switch that Regulates Breast Cancer Stem Cell Fate through WNT Signaling.

    PubMed

    El Helou, Rita; Pinna, Guillaume; Cabaud, Olivier; Wicinski, Julien; Bhajun, Ricky; Guyon, Laurent; Rioualen, Claire; Finetti, Pascal; Gros, Abigaelle; Mari, Bernard; Barbry, Pascal; Bertucci, Francois; Bidaut, Ghislain; Harel-Bellan, Annick; Birnbaum, Daniel; Charafe-Jauffret, Emmanuelle; Ginestier, Christophe

    2017-02-28

    Breast cancer stem cells (bCSCs) have been implicated in tumor progression and therapeutic resistance; however, the molecular mechanisms that define this state are unclear. We have performed two microRNA (miRNA) gain- and loss-of-function screens to identify miRNAs that regulate the choice between bCSC self-renewal and differentiation. We find that micro-RNA (miR)-600 silencing results in bCSC expansion, while its overexpression reduces bCSC self-renewal, leading to decreased in vivo tumorigenicity. miR-600 targets stearoyl desaturase 1 (SCD1), an enzyme required to produce active, lipid-modified WNT proteins. In the absence of miR-600, WNT signaling is active and promotes self-renewal, whereas overexpression of miR-600 inhibits the production of active WNT and promotes bCSC differentiation. In a series of 120 breast tumors, we found that a low level of miR-600 is correlated with active WNT signaling and a poor prognosis. These findings highlight a miR-600-centered signaling network that governs bCSC-fate decisions and influences tumor progression.

  19. Regulation D Study Act

    THOMAS, 113th Congress

    Rep. Pittenger, Robert [R-NC-9

    2013-10-03

    12/03/2014 Received in the Senate and Read twice and referred to the Committee on Banking, Housing, and Urban Affairs. (All Actions) Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  20. Bacillus subtilis glutamine synthetase regulates its own synthesis by acting as a chaperone to stabilize GlnR-DNA complexes.

    PubMed

    Fisher, Susan H; Wray, Lewis V

    2008-01-22

    The Bacillus subtilis GlnR repressor controls gene expression in response to nitrogen availability. Because all GlnR-regulated genes are expressed constitutively in mutants lacking glutamine synthetase (GS), GS is required for repression by GlnR. Feedback-inhibited GS (FBI-GS) was shown to activate GlnR DNA binding with an in vitro electophoretic mobility shift assay (EMSA). The activation of GlnR DNA binding by GS in these experiments depended on the feedback inhibitor glutamine and did not occur with mutant GS proteins defective in regulating GlnR activity in vivo. Although stable GS-GlnR-DNA ternary complexes were not observed in the EMSA experiments, cross-linking experiments showed that a protein-protein interaction occurs between GlnR and FBI-GS. This interaction was reduced in the absence of the feedback inhibitor glutamine and with mutant GS proteins. Because FBI-GS significantly reduced the dissociation rate of the GlnR-DNA complexes, the stability of these complexes is enhanced by FBI-GS. These results argue that FBI-GS acts as a chaperone that activates GlnR DNA binding through a transient protein-protein interaction that stabilizes GlnR-DNA complexes. GS was shown to control the activity of the B. subtilis nitrogen transcription factor TnrA by forming a stable complex between FBI-GS and TnrA that inhibits TnrA DNA binding. Thus, B. subtilis GS is an enzyme with dual catalytic and regulatory functions that uses distinct mechanisms to control the activity of two different transcription factors.

  1. SMG-1 and mTORC1 act antagonistically to regulate response to injury and growth in planarians.

    PubMed

    González-Estévez, Cristina; Felix, Daniel A; Smith, Matthew D; Paps, Jordi; Morley, Simon J; James, Victoria; Sharp, Tyson V; Aboobaker, A Aziz

    2012-01-01

    Planarian flatworms are able to both regenerate their whole bodies and continuously adapt their size to nutrient status. Tight control of stem cell proliferation and differentiation during these processes is the key feature of planarian biology. Here we show that the planarian homolog of the phosphoinositide 3-kinase-related kinase (PIKK) family member SMG-1 and mTOR complex 1 components are required for this tight control. Loss of smg-1 results in a hyper-responsiveness to injury and growth and the formation of regenerative blastemas that remain undifferentiated and that lead to lethal ectopic outgrowths. Invasive stem cell hyper-proliferation, hyperplasia, hypertrophy, and differentiation defects are hallmarks of this uncontrolled growth. These data imply a previously unappreciated and novel physiological function for this PIKK family member. In contrast we found that planarian members of the mTOR complex 1, tor and raptor, are required for the initial response to injury and blastema formation. Double smg-1 RNAi experiments with tor or raptor show that abnormal growth requires mTOR signalling. We also found that the macrolide rapamycin, a natural compound inhibitor of mTORC1, is able to increase the survival rate of smg-1 RNAi animals by decreasing cell proliferation. Our findings support a model where Smg-1 acts as a novel regulator of both the response to injury and growth control mechanisms. Our data suggest the possibility that this may be by suppressing mTOR signalling. Characterisation of both the planarian mTORC1 signalling components and another PIKK family member as key regulators of regeneration and growth will influence future work on regeneration, growth control, and the development of anti-cancer therapies that target mTOR signalling.

  2. SMG-1 and mTORC1 Act Antagonistically to Regulate Response to Injury and Growth in Planarians

    PubMed Central

    González-Estévez, Cristina; Felix, Daniel A.; Smith, Matthew D.; Paps, Jordi; Morley, Simon J.; James, Victoria; Sharp, Tyson V.; Aboobaker, A. Aziz

    2012-01-01

    Planarian flatworms are able to both regenerate their whole bodies and continuously adapt their size to nutrient status. Tight control of stem cell proliferation and differentiation during these processes is the key feature of planarian biology. Here we show that the planarian homolog of the phosphoinositide 3-kinase-related kinase (PIKK) family member SMG-1 and mTOR complex 1 components are required for this tight control. Loss of smg-1 results in a hyper-responsiveness to injury and growth and the formation of regenerative blastemas that remain undifferentiated and that lead to lethal ectopic outgrowths. Invasive stem cell hyper-proliferation, hyperplasia, hypertrophy, and differentiation defects are hallmarks of this uncontrolled growth. These data imply a previously unappreciated and novel physiological function for this PIKK family member. In contrast we found that planarian members of the mTOR complex 1, tor and raptor, are required for the initial response to injury and blastema formation. Double smg-1 RNAi experiments with tor or raptor show that abnormal growth requires mTOR signalling. We also found that the macrolide rapamycin, a natural compound inhibitor of mTORC1, is able to increase the survival rate of smg-1 RNAi animals by decreasing cell proliferation. Our findings support a model where Smg-1 acts as a novel regulator of both the response to injury and growth control mechanisms. Our data suggest the possibility that this may be by suppressing mTOR signalling. Characterisation of both the planarian mTORC1 signalling components and another PIKK family member as key regulators of regeneration and growth will influence future work on regeneration, growth control, and the development of anti-cancer therapies that target mTOR signalling. PMID:22479207

  3. NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival

    PubMed Central

    Calandria, J M; Asatryan, A; Balaszczuk, V; Knott, E J; Jun, B K; Mukherjee, P K; Belayev, L; Bazan, N G

    2015-01-01

    Neuroprotectin D1 (NPD1), a docosahexaenoic acid (DHA)-derived mediator, induces cell survival in uncompensated oxidative stress (OS), neurodegenerations or ischemic stroke. The molecular principles underlying this protection remain unresolved. We report here that, in retinal pigment epithelial cells, NPD1 induces nuclear translocation and cREL synthesis that, in turn, mediates BIRC3 transcription. NPD1 activates NF-κB by an alternate route to canonical signaling, so the opposing effects of TNFR1 and NPD1 on BIRC3 expression are not due to interaction/s between NF-κB pathways. RelB expression follows a similar pattern as BIRC3, indicating that NPD1 also is required to activate cREL-mediated RelB expression. These results suggest that cREL, which follows a periodic pattern augmented by the lipid mediator, regulates a cluster of NPD1-dependent genes after cREL nuclear translocation. BIRC3 silencing prevents NPD1 induction of survival against OS. Moreover, brain NPD1 biosynthesis and selective neuronal BIRC3 abundance are increased by DHA after experimental ischemic stroke followed by remarkable neurological recovery. Thus, NPD1 bioactivity governs key counter-regulatory gene transcription decisive for retinal and brain neural cell integrity when confronted with potential disruptions of homeostasis. PMID:25633199

  4. Negative regulation of transcription in vitro by a glucocorticoid response element is mediated by a trans-acting factor.

    PubMed Central

    Langer, S J; Ostrowski, M C

    1988-01-01

    In vitro experiments with cell extracts prepared from a mouse mammary epithelial cell line demonstrated that a cis-acting glucocorticoid response element (GRE) of the mouse mammary tumor virus represses transcription from its homologous promoter. Competition transcription experiments, in which a molar excess of a restriction fragment that contains the GRE is added to the cell-free assay, revealed that a nuclear factor mediates in trans the negative regulation of mammary tumor virus transcription in vitro. Gel retention assays indicated that a factor in the extracts specifically recognizes the GRE. One unusual result of the gel retention studies was that heating the GRE probe to 65 degrees C before addition to a binding assay increases the formation of the specific protein-DNA complex 20-fold. Exonuclease III footprinting demonstrated that the sequences recognized by the factor are identical for either untreated or heat-treated probe. The footprinting also demonstrated that this factor recognizes sequences that are distinct from those recognized by the glucocorticoid receptor. A synthetic oligonucleotide based on the sequences identified by the footprinting experiments repressed the activity of a heterologous enhancer-promoter in vivo, as assayed by transient expression assays. We propose that this negative transcription element may control the basal level of expression of some glucocorticoid-modulated genes and may explain the insensitivity of certain tumor cells to steroid hormone action. Images PMID:2851730

  5. Price effect of land-use regulation: an event study of the California Coastal Zone Conservation Act

    SciTech Connect

    Yim, H.K.

    1987-01-01

    The passage of the California Coastal Zone Conservation Act (Proposition 20), created regional Coastal Commissions that had veto power over building permits on the coast. The anticipation of restriction in coastal housing construction and the actual regulation by one regional commission, the South Coast Regional Commission, in Los Angeles County, is hypothesized to have impacted the prices of existing single-family houses. An event study methodology is used to construct and test hypotheses of the Commission's price impact. A positive impact on house prices is hypothesized form a combination of the supply restriction and amenity provision activities of the Commission. A negative impact on house price is hypothesized for those houses having the greatest potential for development, whose development was anticipated to have been restricted by the Commission. Results confirm the combined supply/amenity hypothesis and indicate a 6 1/2% price increase associated with the Commission, beginning with the passage of Proposition 20. The development-potential hypothesis was rejected and an alternative hypothesis of a positive impact on high development potential properties accepted. However, it is noted that a sharp decline in the price of high development potential properties occurred during the public debate over proposition 20, which is interpreted as confirmation, within a short-time frame, of the development potential hypothesis.

  6. miR-203 Acts as a Tumor Suppressor Gene in Osteosarcoma by Regulating RAB22A

    PubMed Central

    Yang, Dawei; Liu, Guangpeng; Wang, Kunzheng

    2015-01-01

    microRNAs (miRNAs), small noncoding RNAs of 19–25 nt, play an important roles in the pathological processes of tumorigenesis. The object of this study was to study the expression and function of miR-203 and to found its target gene in osteosarcoma. In our study, we found the expression level of miR-203 was significantly downregulated in osteosarcoma cell lines and tissues. In addition, overexpression of miR-203 inhibited the osteosarcoma cell proliferation and migration and inhibited Mesenchymal-to-Epithelial reversion Transition (MErT). Moreover, we identified RAB22A as a direct target of miR-203 and RAB22A overexpression blocks the roles of miR-203 in osteosarcoma cell. Furthermore, we demonstrated that RAB22A expression was upregulated in human osteosarcoma cell lines and tissues. Take together, our results demonstrated that miR-203 act as a tumor suppressor miRNA through regulating RAB22A expression and suggested its involvement in osteosarcoma progression and carcinogenesis. PMID:26382657

  7. Impacts & Compliance Implementation Plans & Required Deviations for Toxic Substance Control Act (TSCA) Regulation of Double Shell Tanks (DST)

    SciTech Connect

    MULKEY, C.H.

    2000-08-22

    In May 2000, the U.S. Department of Energy, Office of River Protection (DOE-ORP) and the U.S. Environmental Protection Agency (EPA) held meetings regarding the management of polychlorinated biphenyls (PCBs) in the Hanford tank waste. It was decided that the radioactive waste currently stored in the double-shell tanks (DSTs) contain waste which will become subject to the Toxic Substance Control Act (TSCA) (40 CFR 761). As a result, DOE-ORP directed the River Protection Project tank farm contractor (TFC) to prepare plans for managing the PCB inventory in the DSTs. Two components of the PCB management plans are this assessment of the operational impacts of TSCA regulation and the identifications of deviations from TSCA that are required to accommodate tank farm unique limitations. This plan provides ORP and CH2M HILL Hanford Group, Inc. (CHG) with an outline of TSCA PCB requirements and their applicability to tank farm activities, and recommends a compliance/implementation approach. Where strict compliance is not possible, the need for deviations from TSCA PCB requirements is identified. The purpose of assembling this information is to enhance the understanding of PCB management requirements, identify operational impacts and select impact mitigation strategies. This information should be useful in developing formal agreements with EPA where required.

  8. 75 FR 77561 - Regulations Issued Under the Export Grape and Plum Act; Revision to the Minimum Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-13

    ... Grape and Plum Act; Revision to the Minimum Requirements AGENCY: Agricultural Marketing Service, USDA... under the Export Grape and Plum Act. The proposed action would change the minimum bunch weight...: This proposed rule is issued under authority of the Export Grape and Plum Act, as amended (7 U.S.C....

  9. Federal Register Notice: State Authorization To Regulate the Hazardous Components of Radioactive Mixed Wastes Under the Resource Conservation and Recovery Act

    EPA Pesticide Factsheets

    The Environmental Protection Agency (EPA) is today publishing a notice that in order to obtain and maintain authorization to administer and enforce a hazardous waste program pursuant to Subtitle C of the Resource Conservation and Recovery Act (RCRA), States must have authority to regulate the hazardous components of 'radioactive mixed wastes.

  10. 28 CFR Appendix D to Subpart G of... - OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) D Appendix D to Subpart G of Part 42 Judicial Administration DEPARTMENT OF JUSTICE NONDISCRIMINATION; EQUAL..., App. D Appendix D to Subpart G of Part 42—OJARS' Regulations Under the Omnibus Crime Control and...

  11. 20 CFR 221.4 - When a jurisdiction decision may be reversed.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false When a jurisdiction decision may be reversed. 221.4 Section 221.4 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD RETIREMENT ACT JURISDICTION DETERMINATIONS § 221.4 When a jurisdiction decision may be reversed. The...

  12. 29 CFR 1981.110 - Decision and orders of the Administrative Review Board.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 9 2011-07-01 2011-07-01 false Decision and orders of the Administrative Review Board. 1981.110 Section 1981.110 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND... COMPLAINTS UNDER SECTION 6 OF THE PIPELINE SAFETY IMPROVEMENT ACT OF 2002 Litigation § 1981.110 Decision...

  13. 29 CFR 1981.109 - Decision and orders of the administrative law judge.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 9 2013-07-01 2013-07-01 false Decision and orders of the administrative law judge. 1981.109 Section 1981.109 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH... SECTION 6 OF THE PIPELINE SAFETY IMPROVEMENT ACT OF 2002 Litigation § 1981.109 Decision and orders of...

  14. 29 CFR 1981.109 - Decision and orders of the administrative law judge.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 9 2011-07-01 2011-07-01 false Decision and orders of the administrative law judge. 1981.109 Section 1981.109 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH... SECTION 6 OF THE PIPELINE SAFETY IMPROVEMENT ACT OF 2002 Litigation § 1981.109 Decision and orders of...

  15. 29 CFR 1981.110 - Decision and orders of the Administrative Review Board.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 9 2014-07-01 2014-07-01 false Decision and orders of the Administrative Review Board. 1981.110 Section 1981.110 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND... COMPLAINTS UNDER SECTION 6 OF THE PIPELINE SAFETY IMPROVEMENT ACT OF 2002 Litigation § 1981.110 Decision...

  16. 29 CFR 1981.109 - Decision and orders of the administrative law judge.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 9 2012-07-01 2012-07-01 false Decision and orders of the administrative law judge. 1981.109 Section 1981.109 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH... SECTION 6 OF THE PIPELINE SAFETY IMPROVEMENT ACT OF 2002 Litigation § 1981.109 Decision and orders of...

  17. 29 CFR 1981.109 - Decision and orders of the administrative law judge.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 9 2014-07-01 2014-07-01 false Decision and orders of the administrative law judge. 1981.109 Section 1981.109 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH... SECTION 6 OF THE PIPELINE SAFETY IMPROVEMENT ACT OF 2002 Litigation § 1981.109 Decision and orders of...

  18. 20 CFR 703.204 - Decision on insurance carrier's application; minimum amount of deposit.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Decision on insurance carrier's application..., DEPARTMENT OF LABOR LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED STATUTES INSURANCE REGULATIONS Insurance Carrier Security Deposit Requirements § 703.204 Decision on insurance...

  19. 10 CFR 780.35 - Communication of decision to General Counsel.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Communication of decision to General Counsel. 780.35 Section 780.35 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.35 Communication of decision to...

  20. 10 CFR 780.46 - Communication of decision to General Counsel.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Communication of decision to General Counsel. 780.46 Section 780.46 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153c of the Atomic Energy Act of 1954 § 780.46 Communication of decision to...

  1. 10 CFR 780.46 - Communication of decision to General Counsel.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Communication of decision to General Counsel. 780.46 Section 780.46 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153c of the Atomic Energy Act of 1954 § 780.46 Communication of decision to...

  2. 10 CFR 780.34 - Criteria for decision to issue a license.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Criteria for decision to issue a license. 780.34 Section 780.34 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.34 Criteria for decision to issue...

  3. 10 CFR 780.35 - Communication of decision to General Counsel.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Communication of decision to General Counsel. 780.35 Section 780.35 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.35 Communication of decision to...

  4. 10 CFR 780.34 - Criteria for decision to issue a license.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Criteria for decision to issue a license. 780.34 Section 780.34 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.34 Criteria for decision to issue...

  5. 13 CFR 142.31 - Can a party request reconsideration of the initial decision?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Can a party request reconsideration of the initial decision? 142.31 Section 142.31 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 142.31 Can a party...

  6. 13 CFR 142.32 - When does the initial decision of the ALJ become final?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false When does the initial decision of the ALJ become final? 142.32 Section 142.32 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 142.32 When does the...

  7. 13 CFR 142.33 - What are the procedures for appealing the ALJ decision?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are the procedures for appealing the ALJ decision? 142.33 Section 142.33 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION PROGRAM FRAUD CIVIL REMEDIES ACT REGULATIONS Decisions and Appeals § 142.33 What are...

  8. ANNOTATION TAKEN, IN THE PERSPECTIVE OF CRIMINAL AND CONSTITUTIONAL LAW, AS WELL AS IN CRIMINOLOGY, TO THE DECISION OF THE PORTUGUESE CONSTITUTIONAL COURT, OF JANUARY 13, 2011--WITH RESPECT TO THE PROBLEMS OF "CONSENT" AND "MEDICAL ACT".

    PubMed

    Bandeira, Gonçalo S de Melo

    2014-07-01

    1--Summary of the decision taken by the Portuguese Constitutional Court, of January 13, 2011; 2--Complete text of the decision of the Portuguese Constitutional Court, of January 13, 2011, Judge Maria João ANTUNES (Reporter), Judge Carlos Pamplona de OLIVEIRA, Judge José Borges SOEIRO, Judge Gil GALVÃO, Judge Rui Manuel Moura RAMOS (President)--in terms of the appositive declaration to the sentence n. 487/2010: t.c.http://www. tribunalconstitucional.pt, August 1, 2011; 3--Brief annotation to the problem of the "medical act"; 3.1--Plus some conclusions on the brief annotation to the problem of the "medical act"; 3.2--Brief annotation to the problem of "consent"--continuation of the previous comments; 4--Conclusions. It must never be forgotten that "consent" does not stand as the only cause of exclusion of unlawfulness.

  9. Decision Point 1 of Statement of Project Objectives (SOPO)Recovery Act: Development of ITM Oxygen Technology for Integration with Advanced Industrial Systems

    SciTech Connect

    Armstrong, Phillip

    2011-04-11

    Air Products is designing, building, and operating a ceramic membrane fabrication facility (the “CerFab”) to enable production of membrane modules to supply a conceptual 2000 ton per day (TPD) ITM Oxygen facility (the “ITM Oxygen Development Facility”). Air Products is executing this project under the American Recovery and Reinvestment Act (ARRA) with the objective to accelerate the adoption of ITM Oxygen technology to help meet the country’s goals for deploying clean power plants. The objective of this Topical Report is to address the requirements of Decision Point 1, which pertains to the Site Selection for the CerFab. Air Products with subcontractor Ceramatec, Inc., has determined a ceramic membrane module manufacturing process and the building and site requirements of the process consistent with the manufacturing objectives of the ITM Oxygen development program and this ARRA project. Based on cost estimates for new construction and refurbishments of existing sites, Air Products chose to consider only existing buildings rather than new construction. The Salt Lake City, Utah, region was selected as the preferred location for the CerFab to enable Ceramatec, Inc., to support the Phase V project and on-going Phase III development activities. Twenty available properties were screened in this region, from which one property emerged clearly as the most suitable property to house the CerFab. The site meets the requirements of the CerFab in terms of floor space, power and other utilities, and building structural features. The site also meets criteria for ease of obtaining the requirement environmental permits, and is within the project cost allocation for the building site. Moreover, the site formerly housed a manufacturing company that ceased operations in 2010 as a result of the economic recession of 2008-9; the region of the site has experienced more than a doubling of the unemployment rate since 2007. Restarting a manufacturing operation in the same

  10. 32 CFR 1801.45 - Notification of decision and right of judicial review.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Notification of decision and right of judicial review. 1801.45 Section 1801.45 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Administrative...

  11. 32 CFR 1801.23 - Notification of decision and right of appeal.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Notification of decision and right of appeal. 1801.23 Section 1801.23 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Requests §...

  12. 32 CFR 1801.45 - Notification of decision and right of judicial review.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Notification of decision and right of judicial review. 1801.45 Section 1801.45 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Administrative...

  13. 32 CFR 1801.45 - Notification of decision and right of judicial review.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Notification of decision and right of judicial review. 1801.45 Section 1801.45 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Administrative...

  14. 32 CFR 1801.23 - Notification of decision and right of appeal.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Notification of decision and right of appeal. 1801.23 Section 1801.23 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Requests §...

  15. 32 CFR 1801.23 - Notification of decision and right of appeal.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Notification of decision and right of appeal. 1801.23 Section 1801.23 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Requests §...

  16. 32 CFR 1801.23 - Notification of decision and right of appeal.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Notification of decision and right of appeal. 1801.23 Section 1801.23 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Requests §...

  17. 32 CFR 1801.23 - Notification of decision and right of appeal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Notification of decision and right of appeal. 1801.23 Section 1801.23 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Requests §...

  18. 32 CFR 1801.45 - Notification of decision and right of judicial review.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Notification of decision and right of judicial review. 1801.45 Section 1801.45 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Administrative...

  19. 32 CFR 1801.45 - Notification of decision and right of judicial review.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Notification of decision and right of judicial review. 1801.45 Section 1801.45 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action On Privacy Act Administrative...

  20. The First Amendment in Higher Education: A Review of the 1995 Judicial Decisions.

    ERIC Educational Resources Information Center

    O'Shea, Kevin F.

    1997-01-01

    Reviews 1995 court decisions concerning free speech in colleges and universities, focusing on the Free Speech Clause of the First Amendment (educator speech, campus speech regulations, speech in public forum), religion clauses (Establishment Clause, Free Exercise Clause) and statutes (Religious Freedom Restoration Act, Equal Access Act). Concludes…

  1. 32 CFR 1901.23 - Notification of decision and right of appeal.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Notification of decision and right of appeal. 1901.23 Section 1901.23 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests §...

  2. 32 CFR 1901.45 - Notification of decision and right of judicial review.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Notification of decision and right of judicial review. 1901.45 Section 1901.45 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Administrative...

  3. 32 CFR 1901.23 - Notification of decision and right of appeal.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Notification of decision and right of appeal. 1901.23 Section 1901.23 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests §...

  4. 32 CFR 1901.45 - Notification of decision and right of judicial review.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Notification of decision and right of judicial review. 1901.45 Section 1901.45 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Administrative...

  5. Preventing risk for obesity by promoting self-regulation and decision-making skills: pilot results from the PATHWAYS to health program (PATHWAYS).

    PubMed

    Riggs, Nathaniel R; Sakuma, Kari-Lyn Kobayakawa; Pentz, Mary Ann

    2007-06-01

    The overall aim of the two school-based pilot studies was to evaluate whether an approach to prevention that focused on changing child impulse control, decision making, and social competence can be effective in changing attitudes toward food intake and physical activity as risk factors for obesity. The strategy used was to translate specific components of one evidence-based program for violence prevention (Promoting Alternative Thinking Strategies: PATHS) into an elementary school curriculum program for obesity prevention. Both studies demonstrated significant changes in positive attitudes toward self-regulation of appetitive behavior. In addition, Study 2 demonstrated positive changes in actual food choices and television viewing patterns. Implications are that comprehensive efforts to prevent youth risk for obesity should include as one component school-based curricula that target self-regulation and decision-making skills.

  6. 20 CFR 349.8 - Discretion of the three-member Board to reopen or not to reopen a final decision.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.8 Discretion of the three-member Board to reopen or not...

  7. 20 CFR 349.8 - Discretion of the three-member Board to reopen or not to reopen a final decision.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.8 Discretion of the three-member Board to reopen or not...

  8. 20 CFR 349.8 - Discretion of the three-member Board to reopen or not to reopen a final decision.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... BOARD REGULATIONS UNDER THE RAILROAD UNEMPLOYMENT INSURANCE ACT FINALITY OF DECISIONS REGARDING UNEMPLOYMENT AND SICKNESS INSURANCE BENEFITS § 349.8 Discretion of the three-member Board to reopen or not...

  9. A demonstration of shared decision making in primary care highlights barriers to adoption and potential remedies.

    PubMed

    Friedberg, Mark W; Van Busum, Kristin; Wexler, Richard; Bowen, Megan; Schneider, Eric C

    2013-02-01

    Recent developments in health reform related to the passage of the Affordable Care Act and ensuing regulations encourage delivery systems to engage in shared decision making, in which patients and providers together make health care decisions that are informed by medical evidence and tailored to the specific characteristics and values of the patient. To better understand how delivery systems can implement shared decision making, we interviewed representatives of eight primary care sites participating in a demonstration funded and coordinated by the Informed Medical Decisions Foundation. Barriers to shared decision making included overworked physicians, insufficient provider training, and clinical information systems incapable of prompting or tracking patients through the decision-making process. Methods to improve shared decision making included using automatic triggers for the distribution of decision aids and engaging team members other than physicians in the process. We conclude that substantial investments in provider training, information systems, and process reengineering may be necessary to implement shared decision making successfully.

  10. Decision Point 3 of Statement of Project Objectives (SOPO) “Recovery Act: Development of ITM Oxygen Technology for Integration with Advanced Industrial Systems”

    SciTech Connect

    Armstrong, Phillip

    2012-03-01

    Air Products is carrying out a scope of work under Phase 5 of the ITM Oxygen Cooperative Agreement to design, build, and operate a ceramic membrane fabrication facility (the -CerFabII) to enable production of membrane modules to supply a conceptual 2000 ton per day (TPD) ITM Oxygen facility (the -ITM Oxygen Development FacilityII), and to perform supporting development tasks in materials development and engineering development toward industrial, carbon capture and sequestration applications. Air Products is executing this project under the American Recovery and Reinvestment Act (ARRA) with the objective to accelerate the adoption of ITM Oxygen technology to help meet the country’s goals for deploying clean power plants. The objective of this Topical Report is to address the requirements of Decision Point 3 (DP3), which pertains to the status of all Tasks within Phase 5 and most notably the project status of the CerFab (Task 30) prior to authorization of funds for equipment purchase and construction of the facility. The intent of the DP3 is to provide the opportunity for DOE-NETL to review the status of these tasks and to make recommendations on forward project direction, including a recommendation to pass into Budget Period 8. In the area of Materials Development, Air Products has specified a high pressure dilatometer system which will enable measurements of material expansion of ITM ceramic compounds at very high oxygen partial pressures consistent with CCS applications. Under Task 28.2, subcontractor Ceramatec has made significant progress since DP2 in materials selection and process development and improvement for advanced architecture module fabrication. Ceramatec has determined a materials specification, and has selected a process for making the material. Ceramatec has further developed and selected the process for applying the membrane to unsintered advanced architecture wafers with a Two Step process. Ceramatec has built submodules meeting leak rate

  11. Transcription Factors SOD7/NGAL2 and DPA4/NGAL3 Act Redundantly to Regulate Seed Size by Directly Repressing KLU Expression in Arabidopsis thaliana

    PubMed Central

    Zhang, Yueying; Du, Liang; Xu, Ran; Cui, Rongfeng; Hao, Jianjun; Sun, Caixia; Li, Yunhai

    2015-01-01

    Although seed size is one of the most important agronomic traits in plants, the genetic and molecular mechanisms that set the final size of seeds are largely unknown. We previously identified the ubiquitin receptor DA1 as a negative regulator of seed size, and the Arabidopsis thaliana da1-1 mutant produces larger seeds than the wild type. Here, we describe a B3 domain transcriptional repressor NGATHA-like protein (NGAL2), encoded by the suppressor of da1-1 (SOD7), which acts maternally to regulate seed size by restricting cell proliferation in the integuments of ovules and developing seeds. Overexpression of SOD7 significantly decreases seed size of wild-type plants, while the simultaneous disruption of SOD7 and its closest homolog DEVELOPMENT-RELATED PcG TARGET IN THE APEX4 (DPA4/NGAL3) increases seed size. Genetic analyses indicate that SOD7 and DPA4 act in a common pathway with the seed size regulator KLU to regulate seed growth, but do so independently of DA1. Further results show that SOD7 directly binds to the promoter of KLUH (KLU) in vitro and in vivo and represses the expression of KLU. Therefore, our findings reveal the genetic and molecular mechanisms of SOD7, DPA4, and KLU in seed size regulation and suggest that they are promising targets for seed size improvement in crops. PMID:25783029

  12. 45 CFR 1703.203 - Decision to close meeting.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....203 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Procedures Governing Decisions About Meetings... matters and is scheduled to be held no more than thirty days after the initial meeting in such series....

  13. 45 CFR 1703.203 - Decision to close meeting.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....203 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Procedures Governing Decisions About Meetings... matters and is scheduled to be held no more than thirty days after the initial meeting in such series....

  14. 45 CFR 1703.203 - Decision to close meeting.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....203 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Procedures Governing Decisions About Meetings... matters and is scheduled to be held no more than thirty days after the initial meeting in such series....

  15. Negotiation and Decision Making with Collaborative Software: How MarineMap `Changed the Game' in California's Marine Life Protected Act Initiative

    NASA Astrophysics Data System (ADS)

    Cravens, Amanda E.

    2016-02-01

    Environmental managers and planners have become increasingly enthusiastic about the potential of decision support tools (DSTs) to improve environmental decision-making processes as information technology transforms many aspects of daily life. Discussions about DSTs, however, rarely recognize the range of ways software can influence users' negotiation, problem-solving, or decision-making strategies and incentives, in part because there are few empirical studies of completed processes that used technology. This mixed-methods study—which draws on data from approximately 60 semi-structured interviews and an online survey—examines how one geospatial DST influenced participants' experiences during a multi-year marine planning process in California. Results suggest that DSTs can facilitate communication by creating a common language, help users understand the geography and scientific criteria in play during the process, aid stakeholders in identifying shared or diverging interests, and facilitate joint problem solving. The same design features that enabled the tool to aid in decision making, however, also presented surprising challenges in certain circumstances by, for example, making it difficult for participants to discuss information that was not spatially represented on the map-based interface. The study also highlights the importance of the social context in which software is developed and implemented, suggesting that the relationship between the software development team and other participants may be as important as technical software design in shaping how DSTs add value. The paper concludes with considerations to inform the future use of DSTs in environmental decision-making processes.

  16. Negotiation and Decision Making with Collaborative Software: How MarineMap 'Changed the Game' in California's Marine Life Protected Act Initiative.

    PubMed

    Cravens, Amanda E

    2016-02-01

    Environmental managers and planners have become increasingly enthusiastic about the potential of decision support tools (DSTs) to improve environmental decision-making processes as information technology transforms many aspects of daily life. Discussions about DSTs, however, rarely recognize the range of ways software can influence users' negotiation, problem-solving, or decision-making strategies and incentives, in part because there are few empirical studies of completed processes that used technology. This mixed-methods study-which draws on data from approximately 60 semi-structured interviews and an online survey--examines how one geospatial DST influenced participants' experiences during a multi-year marine planning process in California. Results suggest that DSTs can facilitate communication by creating a common language, help users understand the geography and scientific criteria in play during the process, aid stakeholders in identifying shared or diverging interests, and facilitate joint problem solving. The same design features that enabled the tool to aid in decision making, however, also presented surprising challenges in certain circumstances by, for example, making it difficult for participants to discuss information that was not spatially represented on the map-based interface. The study also highlights the importance of the social context in which software is developed and implemented, suggesting that the relationship between the software development team and other participants may be as important as technical software design in shaping how DSTs add value. The paper concludes with considerations to inform the future use of DSTs in environmental decision-making processes.

  17. Federal regulation comes to private health care financing: the group health insurance provisions of the Health Insurance Portability and Accountability Act of 1996.

    PubMed

    Rovner, J A

    1998-01-01

    Attorney Rovner presents a very detailed accounting of the impacts of the Health Insurance Portability and Accountability Act as it relates to group health insurance including provisions that concern pre-existing conditions, special enrollment rights, premium discrimination, maternity lengths of stay, parity for mental health benefits and small groups coverage. The article concludes with a discussion of the federalism question as it relates to regulation of private market health financing.

  18. Guidance for applicants for state wellhead protection program assistance funds under the Safe Drinking Water Act. Appendix C. Wellhead Protection Program-applicable regulations

    SciTech Connect

    Not Available

    1987-06-01

    Appendix C is a companion document to the Guidance for Applicants for State Wellhead Protection Assistance Funds Under the Safe Drinking Water Act to explain EPA regulations applicable to the Wellhead Protection Program (WHP) and the assistance application form needed to request a program grant. These two documents are used to develop approvable assistance applications and to administer properly the funds awarded under the WHP program.

  19. Early Warning Report: Use of Contractors to Conduct Clean Air Act Risk Management Program Inspections in Certain States Goes Against Court Decisions

    EPA Pesticide Factsheets

    Report #12-P-0376, March 28, 2012. The OIG is currently evaluating whether the EPA has adequate management controls for ensuring the effectiveness of its Clean Air Act (CAA) Section 112(r) risk management program inspections.

  20. Regulators as agents: modelling personality and power as evidence is brokered to support decisions on environmental risk.

    PubMed

    Davies, G J; Kendall, G; Soane, E; Li, J; Rocks, S A; Jude, S R; Pollard, S J T

    2014-01-01

    Complex regulatory decisions about risk rely on the brokering of evidence between providers and recipients, and involve personality and power relationships that influence the confidence that recipients may place in the sufficiency of evidence and, therefore, the decision outcome. We explore these relationships in an agent-based model; drawing on concepts from environmental risk science, decision psychology and computer simulation. A two-agent model that accounts for the sufficiency of evidence is applied to decisions about salt intake, animal carcass disposal and radioactive waste. A dynamic version of the model assigned personality traits to agents, to explore their receptivity to evidence. Agents with 'aggressor' personality sets were most able to imbue fellow agents with enhanced receptivity (with 'avoider' personality sets less so) and clear confidence in the sufficiency of evidence. In a dynamic version of the model, when both recipient and provider were assigned the 'aggressor' personality set, this resulted in 10 successful evidence submissions in 71 days, compared with 96 days when both agents were assigned the 'avoider' personality set. These insights suggest implications for improving the efficiency and quality of regulatory decision making by understanding the role of personality and power.