Science.gov

Sample records for act regulations requirements

  1. 78 FR 11279 - Loan Originator Compensation Requirements Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-15

    ...The Bureau of Consumer Financial Protection (Bureau) is amending Regulation Z to implement amendments to the Truth in Lending Act (TILA) made by the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act). The final rule implements requirements and restrictions imposed by the Dodd-Frank Act concerning loan originator compensation; qualifications of, and registration or......

  2. 78 FR 4725 - Escrow Requirements Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ...The Bureau of Consumer Financial Protection (Bureau) is publishing a final rule that amends Regulation Z (Truth in Lending) to implement certain amendments to the Truth in Lending Act made by the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act). Regulation Z currently requires creditors to establish escrow accounts for higher-priced mortgage loans secured by a first......

  3. 21 CFR 16.24 - Regulatory hearing required by the act or a regulation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Regulatory hearing required by the act or a regulation. 16.24 Section 16.24 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL REGULATORY HEARING BEFORE THE FOOD AND DRUG ADMINISTRATION Initiation of Proceedings §...

  4. 75 FR 35338 - Implementation of Regulations Required Under Title XI of the Food, Conservation and Energy Act of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-22

    ...-AB07 Implementation of Regulations Required Under Title XI of the Food, Conservation and Energy Act of... participants regarding compliance. In enacting Title XI of the Food, Conservation and Energy Act of 2008 (Farm..., and its coverage was to encompass the complete chain of commerce and give the Secretary of...

  5. Cis-acting elements are required for selenium regulation of glutathione peroxidase-1 mRNA levels.

    PubMed Central

    Weiss, S L; Sunde, R A

    1998-01-01

    Classical glutathione peroxidase (GPX1) mRNA levels can decrease to less than 10% in selenium (Se)-deficient rat liver. The cis-acting nucleic acid sequence requirements for Se regulation of GPX1 mRNA levels were studied by transfecting Chinese hamster ovary (CHO) cells with GPX1 DNA constructs in which specific regions of the GPX1 gene were mutated, deleted, or replaced by comparable regions from unregulated genes such as phospholipid hydroperoxide glutathione peroxidase (GPX4). For each construct, stable transfectants were pooled two weeks after transfection, divided into Se-deficient (2 nM Se) or Se-adequate (200 nM Se) medium, and grown for an additional four days. On day of harvest, Se-deficient GPX1 and GPX4 activities averaged 13 +/- 2% and 15 +/- 2% of Se adequate levels, confirming that cellular Se status was dramatically altered by Se supplementation. RNA was isolated from replicate plates of cells and transfected mRNA levels were specifically determined by RNase protection assay. Analysis of chimeric GPX1/GPX4 constructs showed that the GPX4 3'-UTR can completely replace the GPX1 3'-UTR in Se regulation of GPX1 mRNA. We did not find any GPX1 coding regions that could be replaced by the corresponding GPX4 coding regions without diminishing or eliminating Se regulation of the transfected GPX1 mRNA. Further analysis of the GPX1 coding region demonstrated that the GPX1 Sec codon (UGA) and the GPX1 intron sequences are required for full Se regulation of transfected GPX1 mRNA levels. Mutations that moved the GPX1 Sec codon to three different positions within the GPX1 coding region suggest that the mechanism for Se regulation of GPX1 mRNA requires a Sec codon within exon 1. Lastly, we found that addition of the GPX1 3'-UTR to beta-globin mRNA can convey significant Se regulation to beta-globin mRNA levels when a UGA codon is placed within exon 1. We conclude that Se regulation of GPX1 mRNA requires a functional selenocysteine insertion sequence (SECIS) in the 3

  6. Impacts & Compliance Implementation Plans & Required Deviations for Toxic Substance Control Act (TSCA) Regulation of Double Shell Tanks (DST)

    SciTech Connect

    MULKEY, C.H.

    2000-08-22

    In May 2000, the U.S. Department of Energy, Office of River Protection (DOE-ORP) and the U.S. Environmental Protection Agency (EPA) held meetings regarding the management of polychlorinated biphenyls (PCBs) in the Hanford tank waste. It was decided that the radioactive waste currently stored in the double-shell tanks (DSTs) contain waste which will become subject to the Toxic Substance Control Act (TSCA) (40 CFR 761). As a result, DOE-ORP directed the River Protection Project tank farm contractor (TFC) to prepare plans for managing the PCB inventory in the DSTs. Two components of the PCB management plans are this assessment of the operational impacts of TSCA regulation and the identifications of deviations from TSCA that are required to accommodate tank farm unique limitations. This plan provides ORP and CH2M HILL Hanford Group, Inc. (CHG) with an outline of TSCA PCB requirements and their applicability to tank farm activities, and recommends a compliance/implementation approach. Where strict compliance is not possible, the need for deviations from TSCA PCB requirements is identified. The purpose of assembling this information is to enhance the understanding of PCB management requirements, identify operational impacts and select impact mitigation strategies. This information should be useful in developing formal agreements with EPA where required.

  7. 77 FR 33638 - Amendment to the Bank Secrecy Act Regulations-Exemption From the Requirement To Report...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ...FinCEN is issuing this final rule to amend the regulations that allow depository institutions to exempt transactions of certain payroll customers \\1\\ from the requirement to report transactions in currency in excess of $10,000. The rule substitutes the term ``frequently'' for ``regularly'' in the provision of the exemption rules dealing with payroll customers. This modification of the......

  8. 77 FR 33635 - Amendment to the Bank Secrecy Act Regulations-Requirement That Clerks of Court Report Certain...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ...\\ 66 FR 67680 (December 31, 2001), codified at 31 CFR 1010.330. \\7\\ 26 CFR 1.6050I-1. As amended... Financial Transactions Reporting Act of 1970, as amended by the USA PATRIOT Act of 2001 and other... protect against international terrorism.\\2\\ The Secretary has delegated to the Director of FinCEN...

  9. 75 FR 44163 - Implementation of Regulations Required Under Title XI of the Food, Conservation and Energy Act of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-28

    ... Administration (GIPSA) published a proposed rule in the Federal Register on June 22, 2010 (75 FR 35338) proposing...; ] DEPARTMENT OF AGRICULTURE Grain Inspection, Packers and Stockyards Administration 9 CFR Part 201 RIN 0580... 2008; Conduct in Violation of the Act AGENCY: Grain Inspection, Packers and Stockyards...

  10. Tissue-specific regulation of BiP genes: a cis-acting regulatory domain is required for BiP promoter activity in plant meristems.

    PubMed

    Buzeli, Reginaldo A A; Cascardo, Júlio C M; Rodrigues, Leonardo A Z; Andrade, Maxuel O; Almeida, Raul S; Loureiro, Marcelo E; Otoni, Wagner C; Fontes, Elizabeth P B

    2002-11-01

    The binding protein BiP is an endoplasmic reticulum (ER)-resident member of the HSP70 stress-related protein family, which is essential for the constitutive function of the ER. In addition to responding to a variety of environmental stimuli, plant BiP exhibits a tissue-specific regulation. We have isolated two soybean BiP genomic clones, designated gsBiP6 and gsBiP9, and different extensions of their 5' flanking sequences were fused to beta-glucuronidase (GUS) reporter gene and introduced into Nicotiana tabacum by Agrobacterium tumefaciens-mediated transformation. Transgenic plants displayed prominent GUS activity in the vascular bundles of roots and shoots as well as in regions of intense cell division, such as procambial region and apical meristems. Promoter deletion analyses identified two cis-regulatory functional domains that are important for the spatially-regulated activation of BiP expression under normal plant development. While an AT-rich enhancer-like sequence, designated cis-acting regulatory domain 1, CRD1 (-358 to -211, on gsBiP6), activated expression of the BiP minimal promoter in all organs analyzed, BiP promoter activity in meristematic tissues and phloem cells required the presence of a second activating domain, CRD2 (-211 to -80). Apparently, the CRD2 sequence also harbors negative cis-acting elements, because removal of this region caused activation of gsBiP6 promoter in parenchymatic xylem rays. These results suggest that the tissue-specific control of BiP gene expression requires a complex integration of multiple cis-acting regulatory elements on the promoter. PMID:12374306

  11. 7 CFR 1948.65 - Relocation Act requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 13 2013-01-01 2013-01-01 false Relocation Act requirements. 1948.65 Section 1948.65 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS... Program § 1948.65 Relocation Act requirements. The policies and regulations contained in title 7,...

  12. 7 CFR 1948.65 - Relocation Act requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 13 2012-01-01 2012-01-01 false Relocation Act requirements. 1948.65 Section 1948.65 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS... Program § 1948.65 Relocation Act requirements. The policies and regulations contained in title 7,...

  13. 7 CFR 1948.65 - Relocation Act requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 13 2010-01-01 2009-01-01 true Relocation Act requirements. 1948.65 Section 1948.65 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS... Program § 1948.65 Relocation Act requirements. The policies and regulations contained in title 7,...

  14. 7 CFR 1948.65 - Relocation Act requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 13 2011-01-01 2009-01-01 true Relocation Act requirements. 1948.65 Section 1948.65 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS... Program § 1948.65 Relocation Act requirements. The policies and regulations contained in title 7,...

  15. The French Space Operations Act: Technical Regulations

    NASA Astrophysics Data System (ADS)

    Lazare, B.

    2013-12-01

    The French Space Operations Act (FSOA) [1] stipulates that one of the National Technical Regulations' prime objectives is to protect people, property, public health and the environment. Compliance with these Technical Regulations has been mandatory since 10 December, 2010 for space operations by French space operators and for space operations conducted on French territory. The space safety requirements and regulations governing procedures are based on national and international best practices and experience. A critical design review of the space system and procedures shall be carried out by applicant space operators, in order to verify compliance with the Technical Regulations. An independent technical assessment of the operation is delegated to CNES. The principles applied when drafting the Technical Regulations are as follows: requirements must, as far as possible, establish the rules according to the objective to be obtained, rather than how it is to be achieved; requirements must give preference to international standards recognised as being state of the art; requirements must take previous experience into account. The Technical Regulations are divided into three sections covering requirements common to the launch, control and return of a space object. A special section will cover specific rules to be applied at the Guiana Space Centre. The main topics addressed by the Technical Regulations are: operator safety management system; study of risks to people, property, public health and the Earth's environment; impact study on the outer space environment: space debris generated by the operation; planetary protection. The first version of the Technical Regulations [2], issued in March 2011, is dedicated to unmanned space systems.

  16. 75 FR 63703 - Privacy Act of 1974; Privacy Act Regulation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-18

    ...The Board of Governors of the Federal Reserve System (Board) is issuing a final rule to amend its regulation implementing the Privacy Act of 1974 (Privacy Act). The primary changes concern the waiver of copying fees charged to current and former Board employees, and applicants for Board employment, for access to their records under the Privacy Act; the amendment of special procedures for the......

  17. 30 CFR 252.6 - Freedom of Information Act requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CFR part 2 (Records and Testimony), the requirements of the Act, and the regulations contained in 30 CFR part 250 (Oil and Gas and Sulphur Operations in the Outer Continental Shelf) and 30 CFR part 251... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Freedom of Information Act requirements....

  18. A dietary polyphenol resveratrol acts to provide neuroprotection in recurrent stroke models by regulating AMPK and SIRT1 signaling, thereby reducing energy requirements during ischemia.

    PubMed

    Wang, L-M; Wang, Y-J; Cui, M; Luo, W-J; Wang, X-J; Barber, P A; Chen, Z-Y

    2016-04-01

    The above article from European Journal of Neuroscience, published online on 5 March 2013 in Wiley Online Library (http://onlinelibrary.wiley.com/doi/10.1111/ejn.12162/full), has been retracted by agreement between the Editors-in-Chief, Paul Bolam and John Foxe, the authors and John Wiley & Sons Ltd. The retraction has been agreed as Dr Phillip Barber has informed the publisher that he had seen neither the original data nor any version of the manuscript, and had not been involved in the work reported. A subsequent Institutional investigation found evidence of misconduct on the part of the submitting author. Reference Wang, L.-M., Wang, Y.-J., Cui, M., Luo, W.-J., Wang, X.-J., Barber, P.A. & Chen, Z.-Y. (2013) A dietary polyphenol resveratrol acts to provide neuroprotection in recurrent stroke models by regulating AMPK and SIRT1 signaling, thereby reducing energy requirements during ischemia. Eur. J. Neurosci., 37, 1669-1681. doi: 10.1111/ejn.12162. PMID:27041235

  19. 76 FR 40367 - Federal Acquisition Regulation; Information Collection; Buy American Act-Construction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-08

    ... Regulation; Information Collection; Buy American Act--Construction AGENCY: Department of Defense (DOD... previously approved information collection requirement concerning the Buy American Act--Construction..., American Act--Construction (Grimberg Decision), by any of the following methods: ] Regulations.gov :...

  20. 7 CFR 1948.65 - Relocation Act requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 13 2014-01-01 2013-01-01 true Relocation Act requirements. 1948.65 Section 1948.65 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS-COOPERATIVE SERVICE, RURAL UTILITIES SERVICE, AND FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE (CONTINUED) PROGRAM REGULATIONS...

  1. 75 FR 37287 - Privacy Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-29

    ... April 19, 2010 (75 FR 20298) for a public comment period to end on June 18, 2010. This rule amends the... criminal law enforcement duties. (2) From subsection (c)(4) and (d) because notification would alert a... regulations implementing the Privacy Act of 1974 (Privacy Act), Public Law 93-579, 5 U.S.C. 552a. This...

  2. EPA regulations require close study

    SciTech Connect

    Wilkins, J.D.

    1981-08-03

    The time to review environmental legislation and to pinpoint unreasonable or unnecessary sections is when the proposed regulation is published in the Federal Register before it becomes law. Four oil and gas industry organizations can help track and evaluate environmental regulations: the A.G.A.'s Environmental Coordination Group, the Interstate Natural Gas Association of America (INGAA) Environmental Subcommittee, the Gulf Coast Environmental Affairs Group, and the Houston Environmental Roundtable. Besides acting as a watchdog, the industry must perform independent studies to provide valid data for writing the regulations. Once the laws are passed, the industry has no choice but to comply; a plea of ignorance will not change the situation.

  3. 30 CFR 552.6 - Freedom of Information Act requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... contained in 43 CFR part 2 (Records and Testimony), the requirements of the Act, and the regulations contained in 30 CFR parts 250 and 550 (Oil and Gas and Sulphur Operations in the Outer Continental Shelf) and 30 CFR part 551 (Geological and Geophysical Explorations of the Outer Continental Shelf)....

  4. 30 CFR 252.6 - Freedom of Information Act requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... contained in 43 CFR part 2 (Records and Testimony), the requirements of the Act, and the regulations contained in 30 CFR parts 250 and 550 (Oil and Gas and Sulphur Operations in the Outer Continental Shelf) and 30 CFR parts 251 and 551 (Geological and Geophysical Explorations of the Outer Continental...

  5. 30 CFR 252.6 - Freedom of Information Act requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... contained in 43 CFR part 2 (Records and Testimony), the requirements of the Act, and the regulations contained in 30 CFR parts 250 and 550 (Oil and Gas and Sulphur Operations in the Outer Continental Shelf) and 30 CFR parts 251 and 551 (Geological and Geophysical Explorations of the Outer Continental...

  6. 30 CFR 552.6 - Freedom of Information Act requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... contained in 43 CFR part 2 (Records and Testimony), the requirements of the Act, and the regulations contained in 30 CFR parts 250 and 550 (Oil and Gas and Sulphur Operations in the Outer Continental Shelf) and 30 CFR part 551 (Geological and Geophysical Explorations of the Outer Continental Shelf)....

  7. 77 FR 43081 - Federal Acquisition Regulation; Information Collection; Buy American Act-Free Trade Agreements...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-23

    ... Regulation; Information Collection; Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate... previously approved information collection requirement concerning the Buy American Act--Free Trade Agreements..., Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate, by any of the...

  8. 76 FR 24027 - Federal Acquisition Regulation; Information Collection; Buy American Act Certificate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... Regulation; Information Collection; Buy American Act Certificate AGENCY: Department of Defense (DOD), General... previously approved information collection requirement concerning the Buy American Act certificate. Public... Comment'' screen. Please include your name, company name (if any), and ``Information Collection...

  9. 16 CFR 500.3 - Prohibited acts, coverage, general labeling requirements, exemption procedures.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... requirements, exemption procedures. 500.3 Section 500.3 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENT OF GENERAL POLICY OR INTERPRETATION AND EXEMPTIONS UNDER THE FAIR PACKAGING AND LABELING ACT REGULATIONS UNDER SECTION 4 OF THE FAIR PACKAGING AND LABELING ACT § 500.3 Prohibited...

  10. 16 CFR 500.3 - Prohibited acts, coverage, general labeling requirements, exemption procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... requirements, exemption procedures. 500.3 Section 500.3 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENT OF GENERAL POLICY OR INTERPRETATION AND EXEMPTIONS UNDER THE FAIR PACKAGING AND LABELING ACT REGULATIONS UNDER SECTION 4 OF THE FAIR PACKAGING AND LABELING ACT § 500.3 Prohibited...

  11. 16 CFR 500.3 - Prohibited acts, coverage, general labeling requirements, exemption procedures.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... requirements, exemption procedures. 500.3 Section 500.3 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENT OF GENERAL POLICY OR INTERPRETATION AND EXEMPTIONS UNDER THE FAIR PACKAGING AND LABELING ACT REGULATIONS UNDER SECTION 4 OF THE FAIR PACKAGING AND LABELING ACT § 500.3 Prohibited...

  12. 16 CFR 1500.14 - Products requiring special labeling under section 3(b) of the act.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Products requiring special labeling under section 3(b) of the act. 1500.14 Section 1500.14 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS SUBSTANCES ACT REGULATIONS HAZARDOUS SUBSTANCES AND ARTICLES; ADMINISTRATION AND ENFORCEMENT REGULATIONS § 1500.14...

  13. 76 FR 22070 - Federal Acquisition Regulation; Service Contracts Reporting Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-20

    ...DoD, GSA, and NASA are proposing to amend the Federal Acquisition Regulation (FAR) to implement section 743 of Division C of the Consolidated Appropriations Act, 2010. This proposed rule amends the FAR to require service contractors for executive agencies, except the Department of Defense (DoD), covered by the Federal Activities Inventory Reform (FAIR) Act of 1998, to submit information......

  14. Informational requirements for transcriptional regulation.

    PubMed

    O'Neill, Patrick K; Forder, Robert; Erill, Ivan

    2014-05-01

    Transcription factors (TFs) regulate transcription by binding to specific sites in promoter regions. Information theory provides a useful mathematical framework to analyze the binding motifs associated with TFs but imposes several assumptions that limit their applicability to specific regulatory scenarios. Explicit simulations of the co-evolution of TFs and their binding motifs allow the study of the evolution of regulatory networks with a high degree of realism. In this work we analyze the impact of differential regulatory demands on the information content of TF-binding motifs by means of evolutionary simulations. We generalize a predictive index based on information theory, and we validate its applicability to regulatory scenarios in which the TF binds significantly to the genomic background. Our results show a logarithmic dependence of the evolved information content on the occupancy of target sites and indicate that TFs may actively exploit pseudo-sites to modulate their occupancy of target sites. In regulatory networks with differentially regulated targets, we observe that information content in TF-binding motifs is dictated primarily by the fraction of total probability mass that the TF assigns to its target sites, and we provide a predictive index to estimate the amount of information associated with arbitrarily complex regulatory systems. We observe that complex regulatory patterns can exert additional demands on evolved information content, but, given a total occupancy for target sites, we do not find conclusive evidence that this effect is because of the range of required binding affinities. PMID:24689750

  15. Informational Requirements for Transcriptional Regulation

    PubMed Central

    O'Neill, Patrick K.; Forder, Robert

    2014-01-01

    Abstract Transcription factors (TFs) regulate transcription by binding to specific sites in promoter regions. Information theory provides a useful mathematical framework to analyze the binding motifs associated with TFs but imposes several assumptions that limit their applicability to specific regulatory scenarios. Explicit simulations of the co-evolution of TFs and their binding motifs allow the study of the evolution of regulatory networks with a high degree of realism. In this work we analyze the impact of differential regulatory demands on the information content of TF-binding motifs by means of evolutionary simulations. We generalize a predictive index based on information theory, and we validate its applicability to regulatory scenarios in which the TF binds significantly to the genomic background. Our results show a logarithmic dependence of the evolved information content on the occupancy of target sites and indicate that TFs may actively exploit pseudo-sites to modulate their occupancy of target sites. In regulatory networks with differentially regulated targets, we observe that information content in TF-binding motifs is dictated primarily by the fraction of total probability mass that the TF assigns to its target sites, and we provide a predictive index to estimate the amount of information associated with arbitrarily complex regulatory systems. We observe that complex regulatory patterns can exert additional demands on evolved information content, but, given a total occupancy for target sites, we do not find conclusive evidence that this effect is because of the range of required binding affinities. PMID:24689750

  16. 7 CFR 3575.40 - Equal opportunity and Fair Housing Act requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Equal opportunity and Fair Housing Act requirements. 3575.40 Section 3575.40 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, DEPARTMENT OF AGRICULTURE GENERAL Community Programs Guaranteed Loans § 3575.40 Equal opportunity and Fair Housing Act requirements....

  17. 48 CFR 1480.102 - Buy Indian Act acquisition regulations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Buy Indian Act acquisition... INDIAN AFFAIRS SUPPLEMENT ACQUISITIONS UNDER THE BUY INDIAN ACT General 1480.102 Buy Indian Act... the Buy Indian Act. (b) This part is under the direct oversight and control of the Chief...

  18. Plainer Legal Language: Definitions and Requirements in Acts.

    ERIC Educational Resources Information Center

    Jordan, Michael P.

    1994-01-01

    Explores some of the stylistic complexities of definitions and requirements in a Canadian provincial act. Generates and justifies 15 recommendations for creating a plainer legal language in acts. (SR)

  19. 48 CFR 52.204-11 - American Recovery and Reinvestment Act-Reporting Requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 2 2013-10-01 2013-10-01 false American Recovery and Reinvestment Act-Reporting Requirements. 52.204-11 Section 52.204-11 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses...

  20. 78 FR 39901 - Amendments to the 2013 Mortgage Rules Under the Equal Credit Opportunity Act (Regulation B), Real...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... Bureau issued Escrow Requirements Under the Truth in Lending Act (Regulation Z), 78 FR 4726 (Jan. 30... Settlement Procedures Act (Regulation X), 78 FR 6856 (Jan. 31, 2013) (2013 HOEPA Final Rule), and Ability to Repay and Qualified Mortgage Standards Under the Truth in Lending Act (Regulation Z), 78 FR 6407...

  1. NASA Principal Center for Review of Clean Air Act Regulations

    NASA Technical Reports Server (NTRS)

    Clark-Ingram, Marceia; Munafo, Paul M. (Technical Monitor)

    2002-01-01

    The Clean Air Act (CAA) regulations have greatly impacted materials and processes utilized in the manufacture of aerospace hardware. Code JE/ NASA's Environmental Management Division at NASA Headquarters recognized the need for a formal, Agency-wide review process of CAA regulations. Marshall Space Flight Center (MSFC) was selected as the 'Principal Center for Review of Clean Air Act Regulations'. This presentation describes the centralized support provided by MSFC for the management and leadership of NASA's CAA regulation review process.

  2. 75 FR 35686 - Community Reinvestment Act Regulation Hearings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... TREASURY Office of Thrift Supervision 12 CFR Part 563e Community Reinvestment Act Regulation Hearings... financial institution's performance under the Community Reinvestment Act (CRA). The purpose of the hearings... better serve the goals of the Community Reinvestment Act. The hearings will be held in:...

  3. 48 CFR 1480.102 - Buy Indian Act acquisition regulations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Buy Indian Act acquisition... INDIAN AFFAIRS SUPPLEMENT ACQUISITIONS UNDER THE BUY INDIAN ACT Subpart 1480.1 General 1480.102 Buy... in implementing the Buy Indian Act. (b) This part is under the direct oversight and control of...

  4. 13 CFR 108.30 - Amendments to Act and regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Amendments to Act and regulations. 108.30 Section 108.30 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM Introduction to Part 108 § 108.30 Amendments to Act and regulations. A...

  5. 13 CFR 107.30 - Amendments to Act and regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Amendments to Act and regulations. 107.30 Section 107.30 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Introduction to Part 107 § 107.30 Amendments to Act and regulations. A Licensee shall...

  6. 75 FR 20298 - Privacy Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-19

    ...,'' pursuant to the Privacy Act, as amended (74 FR 60302, Nov. 20, 2009). The Board received no comments on... amendment procedures for this record system. Ivan J. Flores, Paralegal Specialist, Recovery...

  7. 3 CFR - Combating Noncompliance With Recovery Act Reporting Requirements

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 3 The President 1 2011-01-01 2011-01-01 false Combating Noncompliance With Recovery Act Reporting Requirements Presidential Documents Other Presidential Documents Memorandum of April 6, 2010 Combating Noncompliance With Recovery Act Reporting Requirements Memorandum for the Heads of Executive Departments and Agencies My Administration...

  8. 75 FR 5177 - Pipeline Posting Requirements under Section 23 of the Natural Gas Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... clarification of Order No. 720. \\1\\ Pipeline Posting Requirements under section 23 of the Natural Gas Act, 73 FR... Natural Gas Act, 73 FR 1116 (Jan. 7, 2008), FERC Stats. & Regs., Proposed Regulations 2004-2007 ] 32,626.... 670, 71 FR 4244 (Jan. 26, 2006), FERC Stats. & Regs. ] 31,202 (2006). \\44\\ TPA Request for...

  9. 71 FR 34249 - Prescription Drug Marketing Act Pedigree Requirements; Effective Date and Compliance Policy Guide...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2006-06-14

    ...), 1988N-0258 (Formerly 88N- 0258), 2006D-0226] Prescription Drug Marketing Act Pedigree Requirements... provisions of the final regulation published in the Federal Register of ] December 3, 1999 (64 FR 67720). The... of a new compliance policy guide (CPG) 160.900 entitled ``Prescription Drug Marketing Act...

  10. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... credit card practices. (2) The Fair Credit Reporting Act, 15 U.S.C. 1681-1681 setting forth requirements... air carrier and foreign air carrier shall comply with the requirements of Regulation B, 12 CFR part 202, and Regulation Z, 12 CFR part 226, of the Board of Governors of the Federal Reserve Board....

  11. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... credit card practices. (2) The Fair Credit Reporting Act, 15 U.S.C. 1681-1681 setting forth requirements... air carrier and foreign air carrier shall comply with the requirements of Regulation B, 12 CFR part 202, and Regulation Z, 12 CFR part 226, of the Board of Governors of the Federal Reserve Board....

  12. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... credit card practices. (2) The Fair Credit Reporting Act, 15 U.S.C. 1681-1681 setting forth requirements... air carrier and foreign air carrier shall comply with the requirements of Regulation B, 12 CFR part 202, and Regulation Z, 12 CFR part 226, of the Board of Governors of the Federal Reserve Board....

  13. 77 FR 75521 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-21

    ... 1, 2005. 70 FR 44256 (Aug. 2, 2005). As explained in the SUPPLEMENTARY INFORMATION section of these... Home Mortgage Disclosure Act (HMDA). 70 FR 12148 (Mar. 11, 2005). See 12 U.S.C. 2808; 12 CFR 203.2(e)(1... the other federal banking agencies in its CRA rule set forth at 12 CFR part 563e. 72 FR 13429 (Mar....

  14. 75 FR 78932 - Federal Seed Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-17

    ...--Zea mays L. subsp. mays'', ``Crambe--Crambe abyssinica R.E. Fr.'', ``Crotalaria, slenderleaf... Federalism implications. Background The FSA, Title II (7 U.S.C. 1571-1575) regulates agricultural...

  15. 78 FR 32595 - Revision of Freedom of Information Act Regulation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ...This proposed rule would amend HUD's regulations implementing the Freedom of Information Act (FOIA). HUD is proposing these amendments to update and streamline HUD's current FOIA regulation. This proposed rule would update HUD's regulations to reflect statutory changes to FOIA, current HUD organizational structure, and current HUD policies and practices with respect to FOIA. Finally, the rule......

  16. 78 FR 79283 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-30

    ... CRA purposes by the OCC, Board, and FDIC on August 2, 2005, effective September 1, 2005. 70 FR 44256... Act (HMDA). 70 FR 12148 (Mar. 11, 2005). See 12 U.S.C. 2808; 12 CFR 203.2(e)(1). On March 22, 2007... other federal banking agencies in its CRA rule previously set forth at 12 CFR 563e. 72 FR 13429 (Mar....

  17. 76 FR 79529 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... adopted for CRA purposes by the OCC, Board, and FDIC on August 2, 2005, effective September 1, 2005. 70 FR... Disclosure Act (HMDA). 70 FR 12148 (Mar. 22, 2007). See 12 U.S.C. 2808; 12 CFR 203.2(e)(1). On March 22, 2007... federal banking agencies in its CRA rule set forth at 12 CFR 563e. 72 FR 13429. Pursuant to the...

  18. 18 CFR Appendix A to Part 380 - Minimum Filing Requirements for Environmental Reports Under the Natural Gas Act

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Requirements for Environmental Reports Under the Natural Gas Act A Appendix A to Part 380 Conservation of Power... REGULATIONS IMPLEMENTING THE NATIONAL ENVIRONMENTAL POLICY ACT Pt. 380, App. A Appendix A to Part 380—Minimum Filing Requirements for Environmental Reports Under the Natural Gas Act Environmental Reports Under...

  19. 18 CFR Appendix A to Part 380 - Minimum Filing Requirements for Environmental Reports Under the Natural Gas Act

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Requirements for Environmental Reports Under the Natural Gas Act A Appendix A to Part 380 Conservation of Power... REGULATIONS IMPLEMENTING THE NATIONAL ENVIRONMENTAL POLICY ACT Pt. 380, App. A Appendix A to Part 380—Minimum Filing Requirements for Environmental Reports Under the Natural Gas Act Environmental Reports Under...

  20. 78 FR 46555 - Privacy Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-01

    ... File ] System--Interior, FWS-20 (48 FR 54719, December 6, 1983); Bureau of Land Management Criminal... proposing to amend its regulations to exempt certain records in the Incident Management, Analysis and... Management, Analysis and Reporting System (IMARS) system of records. IMARS is an incident management...

  1. Regulations which require employee training: An overview

    SciTech Connect

    Gilfus, L.J.

    1995-11-01

    In order to identify the Occupational Safety and Health Administration (OSHA) regulations which apply to a certain plant or facility, one needs to find the reference materials that are needed and that can be trusted. An overview is presented of the following: Title 29 -- Labor, OSHA Training Requirements; Title 40, Environmental Protection Agency Training Requirements; and Title 49, Department of Transportation Training Regulations.

  2. 25 CFR 273.54 - Privacy Act requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... system of records without prior approval of the Department of Interior and the Office of Management and... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5...

  3. 25 CFR 273.54 - Privacy Act requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... system of records without prior approval of the Department of Interior and the Office of Management and... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5...

  4. 25 CFR 273.54 - Privacy Act requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... system of records without prior approval of the Department of Interior and the Office of Management and... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5...

  5. 25 CFR 273.54 - Privacy Act requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... system of records without prior approval of the Department of Interior and the Office of Management and... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5...

  6. 25 CFR 273.54 - Privacy Act requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... system of records without prior approval of the Department of Interior and the Office of Management and... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5...

  7. 75 FR 36270 - Appraisal Subcommittee; Appraiser Regulation; Privacy Act Implementation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-25

    ... From the Federal Register Online via the Government Publishing Office FEDERAL FINANCIAL INSTITUTIONS EXAMINATION COUNCIL 12 CFR Part 1102 Appraisal Subcommittee; Appraiser Regulation; Privacy Act Implementation AGENCY: Appraisal Subcommittee of the Federal Financial Institutions Examination...

  8. 77 FR 56592 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ... Office of the Secretary 43 CFR Part 2 RIN 1093-AA15 Freedom of Information Act Regulations AGENCY: Office... Department follows in processing records under the Freedom of Information Act (``FOIA''). The revisions clarify and update procedures for requesting information from the Department and procedures that...

  9. 78 FR 18344 - Federal Acquisition Regulation; Submission for OMB Review; Buy American Act-Free Trade Agreements...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-26

    ... Regulation; Submission for OMB Review; Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate... previously approved information collection requirement concerning the Buy American Act--Free Trade Agreements... comments identified by Information Collection 9000- 0130, Buy American Act--Free Trade......

  10. 75 FR 36016 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-24

    .../ neighborhoodspg/arrafactsheet.cfm. \\5\\ 74 FR 21377 (May 7, 2009); 73 FR 58330 (Oct. 6, 2008). Section 2301(c)(3... higher percentages of low- and moderate-income individuals. \\7\\ 70 FR 44256 (Aug. 2, 2005), and 71 FR... requirements of the program, benefit low-, moderate-, and middle-income individuals and geographies...

  11. Brightening up: the effect of the Physician Payment Sunshine Act on existing regulation of pharmaceutical marketing.

    PubMed

    Gorlach, Igor; Pham-Kanter, Genevieve

    2013-01-01

    With the passage of the Physician Payment Sunshine Act as part of the federal health care reform law, pharmaceutical manufacturers are now required to disclose a wide range of payments made by manufacturers to physicians. We review current state regulation of pharmaceutical marketing and consider how the federal sunshine provision will affect existing marketing regulation. We analyze the legal and practical implications of the Physician Payment Sunshine Act. PMID:23581674

  12. 78 FR 6216 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... corrections to the final rule published on December 31, 2012 (77 FR 76898). The regulation revises the... publication on December 31, 2012, of the final rule that was the subject of FR Doc. 2012-31117, is corrected... Office of the Secretary 43 CFR Part 2 RIN 1093-AA15 Freedom of Information Act Regulations AGENCY:...

  13. 77 FR 75362 - Government in the Sunshine Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-20

    ... From the Federal Register Online via the Government Publishing Office EQUAL EMPLOYMENT OPPORTUNITY COMMISSION 29 CFR Part 1612 Government in the Sunshine Act Regulations CFR Correction 0 In Title 29 of the Code of Federal Regulations, Parts 900 to 1899, revised as of July 1, 2012, on page 266, in Sec....

  14. 7 CFR 4290.30 - Amendments to Act and regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4290.30 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL BUSINESS INVESTMENT COMPANY (âRBICâ) PROGRAM Introduction to Part 4290 § 4290.30 Amendments to Act and regulations. A RBIC is subject to...

  15. 76 FR 15236 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-21

    ... Information Fee Schedule and Guidelines published by the Office of Management and Budget at 52 FR 10012 (Mar... Information Act (FOIA). The regulations are being revised to update and streamline the language of several... revisions to the Department's regulations under the FOIA to update and streamline the language of...

  16. 75 FR 22320 - Regulations Implementing the Freedom of Information Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-28

    ... HEALTH REVIEW COMMISSION 29 CFR Part 2201 Regulations Implementing the Freedom of Information Act AGENCY: Occupational Safety and Health Review Commission. ACTION: Notice of proposed rulemaking. SUMMARY: The Occupational Safety and Health Review Commission (OSHRC) is proposing to revise its regulations...

  17. MAPPING GASOLINE REQUIREMENTS, APPLICABLE REGULATIONS AND BANS

    EPA Science Inventory

    Federal and State regulations play an important role in understanding gasoline composition around the United States. Multiple sources of information on these programs were used to develop reliable, up-to-date maps showing gasoline requirements imposed by various regulations. Th...

  18. 76 FR 78977 - Real Estate Settlement Procedures Act (Regulation X)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    ... this rule. See 76 FR 43569 (July 21, 2011). \\11\\ Section 1066 of the Dodd-Frank Act grants the...-Frank Act In developing the interim final rule, the Bureau has conducted an analysis of potential... the described analyses and consultations. Although not required by the interim final rule,...

  19. 48 CFR 52.225-12 - Notice of Buy American Act Requirement-Construction Materials Under Trade Agreements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 2 2013-10-01 2013-10-01 false Notice of Buy American Act... CONTRACT CLAUSES Text of Provisions and Clauses 52.225-12 Notice of Buy American Act Requirement...: Notice of Buy American Act Requirement—Construction Materials Under Trade Agreements (FEB 2009)...

  20. 48 CFR 52.225-12 - Notice of Buy American Act Requirement-Construction Materials Under Trade Agreements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Notice of Buy American Act... CONTRACT CLAUSES Text of Provisions and Clauses 52.225-12 Notice of Buy American Act Requirement...: Notice of Buy American Act Requirement—Construction Materials Under Trade Agreements (FEB 2009)...

  1. 48 CFR 52.225-12 - Notice of Buy American Act Requirement-Construction Materials Under Trade Agreements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false Notice of Buy American Act... CONTRACT CLAUSES Text of Provisions and Clauses 52.225-12 Notice of Buy American Act Requirement...: Notice of Buy American Act Requirement—Construction Materials Under Trade Agreements (FEB 2009)...

  2. 48 CFR 52.225-12 - Notice of Buy American Act Requirement-Construction Materials Under Trade Agreements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 2 2011-10-01 2011-10-01 false Notice of Buy American Act... CONTRACT CLAUSES Text of Provisions and Clauses 52.225-12 Notice of Buy American Act Requirement...: Notice of Buy American Act Requirement—Construction Materials Under Trade Agreements (FEB 2009)...

  3. Emergency Planning and Community Right-to-Know Act (EPCRA) requirements. CERCLA Information Brief

    SciTech Connect

    Dailey, R.

    1993-10-01

    The Emergency Planning and Community Right-to-Know Act (EPCRA), also known as Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA), requires regulated facilities to publicly disclose information about the chemicals they store, use, dispose of, or release. The information is used to encourage and support emergency planning for responding to chemical accidents and to provide local governments and the public with information about possible chemical hazards in their communities.

  4. Clean Air Act requirements for trace-metals information

    SciTech Connect

    Pahl, D.; Hunt, W.; Evans, G.

    1992-01-01

    The Clean Air Act Amendments of 1990 have expanded the requirements for trace metal and air toxics information in urban areas and added new requirements for this information in rural areas and ecosystems. Specific provisions germane to trace metals and other air toxics compounds are found in Title III, Section 112 and in Title IX, Section 901. In response to these provisions, the United States Environmental Protection Agency (EPA) plans to conduct research in atmospheric monitoring networks in urban areas, in the Great Lakes watershed, and in regional components of a national Clean Air Act status and trends network.

  5. HIPAA Mental Health Parity Act--IRS. Notice of proposed rulemaking by cross-reference to temporary regulations.

    PubMed

    1997-12-22

    Elsewhere in this issue of the Federal Register, the IRS is issuing temporary regulations relating to mental health parity requirements imposed on group health plans. These requirements were added to the Internal Revenue Code by section 1532 of the Taxpayer Relief Act of 1997. The IRS is issuing the temporary regulations at the same time that the Pension and Welfare Benefits Administration of the U.S. Department of Labor and the Health Care Financing Administration of the U.S. Department of Health and Human Services are issuing substantially similar interim final regulations relating to mental health parity requirements added by the Mental Health Parity Act of 1996 to the Employee Retirement Income Security Act of 1974 and the Public Health Service Act. The temporary regulations provide guidance to employers and group health plans relating to the new mental health parity requirements. The text of those temporary regulations also serves as the text of these proposed regulations. PMID:10179306

  6. The Health Insurance Portability and Accountability Act: security and privacy requirements.

    PubMed

    Tribble, D A

    2001-05-01

    The security and privacy requirements of the Health Insurance Portability and Accountability Act of 1996 (HIPAA) and their implications for pharmacy are discussed. HIPAA was enacted to improve the portability of health care insurance for persons leaving jobs. A section of the act encourages the use of electronic communications for health care claims adjudication, mandates the use of new standard code sets and transaction sets, and establishes the need for regulations to protect the security and privacy of individually identifiable health care information. Creating these regulations became the task of the Department of Health and Human Services. Regulations on security have been published for comment. Regulations on privacy and the definition of standard transaction sets and code sets are complete. National identifiers for patients, providers, and payers have not yet been established. The HIPAA regulations on security and privacy will require that pharmacies adopt policies and procedures that limit access to health care information. Existing pharmacy information systems may require upgrading or replacement. Costs of implementation nationwide are estimated to exceed $8 billion. The health care community has two years from the finalization of each regulation to comply with that regulation. The security and privacy requirements of HIPAA will require pharmacies to review their practices regarding the storage, use, and disclosure of protected health care information. PMID:11351916

  7. 40 CFR 62.14480 - Does this subpart require me to obtain an operating permit under title V of the Clean Air Act and...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... an operating permit under title V of the Clean Air Act and implementing regulations? 62.14480 Section... the Clean Air Act and implementing regulations? This subpart requires you to obtain an operating permit under title V of the Clean Air Act and implementing regulations (“title V permit”) unless you...

  8. 40 CFR 62.14480 - Does this subpart require me to obtain an operating permit under title V of the Clean Air Act and...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... an operating permit under title V of the Clean Air Act and implementing regulations? 62.14480 Section... the Clean Air Act and implementing regulations? This subpart requires you to obtain an operating permit under title V of the Clean Air Act and implementing regulations (“title V permit”) unless you...

  9. 40 CFR 62.14480 - Does this subpart require me to obtain an operating permit under title V of the Clean Air Act and...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... an operating permit under title V of the Clean Air Act and implementing regulations? 62.14480 Section... the Clean Air Act and implementing regulations? This subpart requires you to obtain an operating permit under title V of the Clean Air Act and implementing regulations (“title V permit”) unless you...

  10. 40 CFR 62.14480 - Does this subpart require me to obtain an operating permit under title V of the Clean Air Act and...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... an operating permit under title V of the Clean Air Act and implementing regulations? 62.14480 Section... the Clean Air Act and implementing regulations? This subpart requires you to obtain an operating permit under title V of the Clean Air Act and implementing regulations (“title V permit”) unless you...

  11. 7 CFR 1437.404 - Information collection requirements under the Paperwork Reduction Act; OMB control number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Information collection requirements under the Paperwork Reduction Act; OMB control number. 1437.404 Section 1437.404 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS NONINSURED...

  12. 29 CFR 1977.3 - General requirements of section 11(c) of the Act.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false General requirements of section 11(c) of the Act. 1977.3 Section 1977.3 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) DISCRIMINATION AGAINST EMPLOYEES EXERCISING RIGHTS UNDER...

  13. 29 CFR 1977.3 - General requirements of section 11(c) of the Act.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 9 2011-07-01 2011-07-01 false General requirements of section 11(c) of the Act. 1977.3 Section 1977.3 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) DISCRIMINATION AGAINST EMPLOYEES EXERCISING RIGHTS UNDER...

  14. 78 FR 80369 - Federal Acquisition Regulation; Service Contracts Reporting Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-31

    ...DoD, GSA, and NASA are issuing a final rule amending the Federal Acquisition Regulation (FAR) to implement a section of the Consolidated Appropriations Act, 2010. This final rule amends the FAR to require service contractors for executive agencies, except where DoD has fully funded the contract or order, to submit information annually in support of agency-level inventories for service......

  15. 75 FR 41370 - Regulations Implementing the Freedom of Information Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ... amended. 75 FR 22320, Apr. 28, 2010. Interested persons were afforded an opportunity to participate in the... HEALTH REVIEW COMMISSION 29 CFR Part 2201 Regulations Implementing the Freedom of Information Act AGENCY: Occupational Safety and Health Review Commission. ACTION: Final rule. SUMMARY: The Occupational Safety...

  16. 76 FR 59071 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... follows: Authority: 50 U.S.C. 401-442; 50 U.S.C. 403a-403v; 5 U.S.C. 552; Executive Order 13392, 70 FR... From the Federal Register Online via the Government Publishing Office CENTRAL INTELLIGENCE AGENCY 32 CFR Part 1900 Freedom of Information Act Regulations AGENCY: Central Intelligence Agency....

  17. 78 FR 50351 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... compliance with, among others, the Comprehensive Plan, principals of good planning and urban design, and...; ] NATIONAL CAPITAL PLANNING COMMISSION 1 CFR Part 456 Freedom of Information Act Regulations AGENCY: National Capital Planning Commission. ACTION: Notice of Proposed Rulemaking. SUMMARY: The National Capital...

  18. 76 FR 45403 - Bank Secrecy Act Regulations-Definitions and Other Regulations Relating to Prepaid Access

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-29

    ... regulations. See 75 FR 65806 (October 26, 2010) (Transfer and Reorganization of Bank Secrecy Act Regulations... Relating to Money Services Businesses, 74 FR 22129 (May 12, 2009), was issued before the Chapter X Final...), (c). \\16\\ 75 FR 36589. B. Prior Regulation of Stored Value In 1999, when FinCEN issued its final...

  19. NASA's Principal Center for Review of Clean Air Act Regulations

    NASA Technical Reports Server (NTRS)

    Clark-Ingram, Marceia

    2003-01-01

    Marshall Space Flight Center (MSFC) was selected as the Principal Center for review of Clean Air Act (CAA) regulations. The CAA Principal Center is tasked to: 1) Provide centralized support to NASA/HDQ Code JE for the management and leadership of NASA's CAA regulation review process; 2) Identify potential impact from proposed CAA regulations to NASA program hardware and supporting facilities. The Shuttle Environmental Assurance Initiative, one of the responsibilities of the NASA CAA Working Group (WG), is described in part of this viewgraph presentation.

  20. 75 FR 19241 - Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations; Defining...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... for Mutual Funds, 67 FR 21117 (April 29, 2002); Customer Identification Programs for Mutual Funds, 68 FR 25131 (May 9, 2003); Amendment to the Bank Secrecy Act Regulations--Requirement That Mutual Funds Report Suspicious Activity, 71 FR 26213 (May 4, 2006); Anti-Money Laundering Programs; Special...

  1. Medical loss ratio regulation under the Affordable Care Act.

    PubMed

    Harrington, Scott E

    2013-01-01

    The minimum medical loss ratio (MLR) regulations in the Affordable Care Act guarantee that a specific percentage of health insurance premiums is spent on medical care and specified activities to improve health care quality. This paper analyzes the regulations' potential unintended consequences and incentive effects, including: higher medical costs and premiums for some insurers; less innovation to align consumer, provider, and health plan incentives, less consumer choice and increased market concentration; and the risk that insurers will pay rebates if claim costs are lower than projected when premiums are established, despite the regulations' permitted "credibility adjustments." The paper discusses modifications and alternatives to the MLR regulations to help achieve their stated goals with less potential for adverse effects. PMID:23720876

  2. Balancing act creating the right regulation for coal combustion waste

    SciTech Connect

    Manuel, J.

    2009-11-15

    The December 2008 collapse of a coal ash pond in Tennessee threw safe management of coal combustion waste (CCW) into the spotlight. Millions of tons of CCW are produced in the United States each year, and a large percentage of that is recycled. The US Environmental Protection Agency is pursuing a host of initiatives that could directly or indirectly affect the disposition of CCW. States, too, are taking a look at how they regulate CCW. Among the options is the possibility of regulating CCW under the Resource Conservation and Recovery Act, a move that could have far-reaching implications for both the recycling and the disposal of this waste.

  3. 77 FR 57043 - Regulations Under the Fur Products Labeling Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... Labeling Act (``TFLA''), and, on its own initiative, deleting unnecessary requirements. \\1\\ 76 FR 13550..., most recently in 1967.\\17\\ \\16\\ 15 U.S.C. 69e(b). \\17\\ 32 FR 6023 (Apr. 15, 1967). C. TFLA In 2010....ftc.gov/opa/2011/07/regreview.shtm . \\24\\ 76 FR 13550. \\25\\ The comments, along with a transcript...

  4. 77 FR 74347 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-14

    ... Information Act (FOIA) (U.S.C. 552) in the Federal Register. See 77 FR 42673, July 20, 2012. CIGIE provided..., Regulatory Planning and Review, 58 FR 51735. This regulation has not been reviewed by the Office of.... Authority: Pub. L. 110-409, 122 Stat. 4302; 5 U.S.C. App; E.O. 12600, 52 FR 23781, 3 CFR, 1987 Comp., p....

  5. General requirements for RCRA regulated hazardous waste tanks

    SciTech Connect

    1995-11-01

    The Resource Conservation and Recovery Act (RCRA), as amended, requires that tanks used for the storage or treatment of hazardous waste (HazW) be permitted, and comply with the requirements contained within the Code of Federal Regulations (CFR) TItle 40 in Subpart J of Part 264/265, unless those tanks have been exempted. Subpart J specifies requirements for the design, construction, installation, operation, inspection, maintenance, repair, release, response, and closure of HazW tanks. Also, the regulations make a distinction between new and existing tanks. Effective December 6, 1995, standards for controlling volatile organic air emissions will apply to non-exempt HazW tanks. HazW tanks will have to be equipped with a cover or floating roof, or be designed to operate as a closed system, to be in compliance with the air emission control requirements. This information brief describes those tanks that are subject to the Subpart J requirements, and will also discuss secondary containment, inspection, restrictions on waste storage, release response, and closure requirements associated with regulated HazW tanks.

  6. 76 FR 43585 - Bank Secrecy Act Regulations; Definitions and Other Regulations Relating to Money Services...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-21

    ...The Financial Crimes Enforcement Network (``FinCEN''), a bureau of the Department of the Treasury (``Treasury''), is revising the regulations implementing the Bank Secrecy Act (``BSA'') regarding money services businesses (``MSBs'') to clarify which entities are covered by the definitions. The changes more clearly delineate the scope of entities regulated as MSBs, so that determining which......

  7. 76 FR 71707 - Revising Underground Storage Tank Regulations-Revisions to Existing Requirements and New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ...EPA is proposing to make certain revisions to the 1988 underground storage tank (UST) technical, financial responsibility, and state program approval regulations. These changes establish federal requirements that are similar to key portions of the Energy Policy Act of 2005; they also update certain 1988 UST regulations. Proposed changes include: Adding secondary containment requirements for......

  8. Additional requirements for charitable hospitals; community health needs assessments for charitable hospitals; requirement of a section 4959 excise tax return and time for filing the return. Final regulations and removal of temporary regulations.

    PubMed

    2014-12-31

    This document contains final regulations that provide guidance regarding the requirements for charitable hospital organizations added by the Patient Protection and Affordable Care Act of 2010. The regulations will affect charitable hospital organizations PMID:25562896

  9. 77 FR 55271 - Truth in Lending Act (Regulation Z); Loan Originator Compensation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-07

    ...The Bureau of Consumer Financial Protection is publishing for public comment a proposed rule amending Regulation Z (Truth in Lending) to implement amendments to the Truth in Lending Act (TILA) made by the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act). The proposal would implement statutory changes made by the Dodd- Frank Act to Regulation Z's current loan......

  10. 7 CFR 800.7 - Information about the Service, Act, and regulations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Information about the Service, Act, and regulations... AGRICULTURE GENERAL REGULATIONS Administration § 800.7 Information about the Service, Act, and regulations. Information about the Grain Inspection, Packers and Stockyards Administration, Service, Act,...

  11. Regulations Under the Americans With Disabilities Act; Genetic Information Nondiscrimination Act. Final rule.

    PubMed

    2016-05-17

    The Equal Employment Opportunity Commission (EEOC or Commission) is issuing its final rule to amend the regulations and interpretive guidance implementing Title I of the Americans with Disabilities Act (ADA) to provide guidance on the extent to which employers may use incentives to encourage employees to participate in wellness programs that ask them to respond to disability-related inquiries and/or undergo medical examinations. This rule applies to all wellness programs that include disability-related inquiries and/or medical examinations whether they are offered only to employees enrolled in an employer-sponsored group health plan, offered to all employees regardless of whether they are enrolled in such a plan, or offered as a benefit of employment by employers that do not sponsor a group health plan or group health insurance. Published elsewhere in this issue of the Federal Register, the EEOC also issued a final rule to amend the regulations implementing Title II of the Genetic Information Nondiscrimination Act (GINA) that addresses the extent to which employers may offer incentives for an employee's spouse to participate in a wellness program. PMID:27192736

  12. Adaptive Equilibrium Regulation: A Balancing Act in Two Timescales

    PubMed Central

    Boker, Steven M.

    2015-01-01

    An equilibrium involves a balancing of forces. Just as one maintains upright posture in standing or walking, many self-regulatory and interpersonal behaviors can be framed as a balancing act between an ever changing environment and within-person processes. The emerging balance between person and environment, the equilibria, are dynamic and adaptive in response to development and learning. A distinction is made between equilibrium achieved solely due to a short timescale balancing of forces and a longer timescale preferred equilibrium which we define as a state towards which the system slowly adapts. Together, these are developed into a framework that this article calls Adaptive Equilibrium Regulation (ÆR), which separates a regulatory process into two timescales: a faster regulation that automatically balances forces and a slower timescale adaptation process that reconfigures the fast regulation so as to move the system towards its preferred equilibrium when an environmental force persists over the longer timescale. This way of thinking leads to novel models for the interplay between multiple timescales of behavior, learning, and development. PMID:27066197

  13. Polypurine/polypyrimidine sequences as cis-acting transcriptional regulators.

    PubMed

    Brahmachari, S K; Sarkar, P S; Raghavan, S; Narayan, M; Maiti, A K

    1997-04-29

    Genome sequence information has generated increasing evidence for the claim that repetitive DNA sequences present within and around genes could play a important role in the regulation of gene expression. Polypurine/polypyrimidine sequences [poly(Pu/Py)] have been observed in the vicinity of promoters and within the transcribed regions of many genes. To understand whether such sequences influence the level of gene expression, we constructed several prokaryotic and eukaryotic expression vectors incorporating poly(Pu/Py) repeats both within and upstream of a reporter gene, lacZ (encoding beta-galactosidase), and studied its expression in vivo. We find that, in contrast to the situation in Escherichia coli, the presence of poly(Pu/Py) sequences within the gene does not significantly inhibit gene expression in mammalian cells. On the other hand, the presence of such sequences upstream of lacZ leads to a several-fold reduction of gene expression in mammalian cells. Similar down-regulation was observed when a structural cassette containing poly(Pu/Py) sequences upstream of lacZ was integrated into yeast chromosome V. Sequence analysis of the nine totally sequenced yeast chromosomes shows that a large number of such sequences occur upstream of ORFs. On the basis of our experimental results and DNA sequence analysis, we propose that these sequences can function as cis-acting transcriptional regulators. PMID:9185844

  14. STENOFOLIA acts as a repressor in regulating leaf blade outgrowth

    PubMed Central

    Lin, Hao; Niu, Lifang; Tadege, Million

    2013-01-01

    We recently reported that the Medicago WOX gene, STENOFOLIA (STF), acts as a transcriptional repressor in regulating leaf blade outgrowth. By using the Nicotiana sylvestris bladeless lam1 mutant as a genetic tool, we showed that the WUS-box, which is conserved among WUS clade WOX genes, is partly responsible for the repressive activity of STF. All members of the modern/WUS clade genes (WUS, WOX1-WOX7) in Arabidopsis that contain intact WUS-box can substitute for STF/LAM1 function while the intermediate and ancient clade members including WOX9,WOX11 and WOX13 cannot, due to lack of the intact WUS-box. Taken together, our results reveal a conserved repression mechanism playing a central role in cell proliferation conferred to the evolutionarily dynamic WOX gene family with acquisition of a repressor domain. PMID:23603965

  15. Actin is required for IFT regulation in Chlamydomonas reinhardtii

    PubMed Central

    Avasthi, Prachee; Onishi, Masayuki; Karpiak, Joel; Yamamoto, Ryosuke; Mackinder, Luke; Jonikas, Martin C.; Sale, Winfield S.; Shoichet, Brian; Pringle, John R.; Marshall, Wallace F.

    2014-01-01

    Summary Assembly of cilia and flagella requires intraflagellar transport (IFT), a highly regulated kinesin-based transport system that moves cargo from the basal body to the tip of flagella [1]. The recruitment of IFT components to basal bodies is a function of flagellar length, with increased recruitment in rapidly growing short flagella [2]. The molecular pathways regulating IFT are largely a mystery. Since actin network disruption leads to changes in ciliary length and number, actin has been proposed to have a role in ciliary assembly. However, the mechanisms involved are unknown. In Chlamydomonas reinhardtii, conventional actin is found in both the cell body and the inner dynein arm complexes within flagella [3, 4]. Previous work showed that treating Chlamydomonas cells with the actin-depolymerizing compound cytochalasin D resulted in reversible flagellar shortening [5], but how actin is related to flagellar length or assembly remains unknown. Here, we utilize small-molecule inhibitors and genetic mutants to analyze the role of actin dynamics in flagellar assembly in Chlamydomonas reinhardtii. We demonstrate that actin plays a role in IFT recruitment to basal bodies during flagellar elongation, and that when actin is perturbed, the normal dependence of IFT recruitment on flagellar length is lost. We also find that actin is required for sufficient entry of IFT material into flagella during assembly. These same effects are recapitulated with a myosin inhibitor suggesting actin may act via myosin in a pathway by which flagellar assembly is regulated by flagellar length. PMID:25155506

  16. Actin is required for IFT regulation in Chlamydomonas reinhardtii.

    PubMed

    Avasthi, Prachee; Onishi, Masayuki; Karpiak, Joel; Yamamoto, Ryosuke; Mackinder, Luke; Jonikas, Martin C; Sale, Winfield S; Shoichet, Brian; Pringle, John R; Marshall, Wallace F

    2014-09-01

    Assembly of cilia and flagella requires intraflagellar transport (IFT), a highly regulated kinesin-based transport system that moves cargo from the basal body to the tip of flagella [1]. The recruitment of IFT components to basal bodies is a function of flagellar length, with increased recruitment in rapidly growing short flagella [2]. The molecular pathways regulating IFT are largely a mystery. Because actin network disruption leads to changes in ciliary length and number, actin has been proposed to have a role in ciliary assembly. However, the mechanisms involved are unknown. In Chlamydomonas reinhardtii, conventional actin is found in both the cell body and the inner dynein arm complexes within flagella [3, 4]. Previous work showed that treating Chlamydomonas cells with the actin-depolymerizing compound cytochalasin D resulted in reversible flagellar shortening [5], but how actin is related to flagellar length or assembly remains unknown. Here we utilize small-molecule inhibitors and genetic mutants to analyze the role of actin dynamics in flagellar assembly in Chlamydomonas reinhardtii. We demonstrate that actin plays a role in IFT recruitment to basal bodies during flagellar elongation and that when actin is perturbed, the normal dependence of IFT recruitment on flagellar length is lost. We also find that actin is required for sufficient entry of IFT material into flagella during assembly. These same effects are recapitulated with a myosin inhibitor, suggesting that actin may act via myosin in a pathway by which flagellar assembly is regulated by flagellar length. PMID:25155506

  17. Ninety-day waiting period limitation and technical amendments to certain health coverage requirements under the Affordable Care Act. Final rule.

    PubMed

    2014-02-24

    These final regulations implement the 90-day waiting period limitation under section 2708 of the Public Health Service Act, as added by the Patient Protection and Affordable Care Act (Affordable Care Act), as amended, and incorporated into the Employee Retirement Income Security Act of 1974 and the Internal Revenue Code. These regulations also finalize amendments to existing regulations to conform to Affordable Care Act provisions. Specifically, these rules amend regulations implementing existing provisions such as some of the portability provisions added by the Health Insurance Portability and Accountability Act of 1996 (HIPAA) because those provisions of the HIPAA regulations have become superseded or require amendment as a result of the market reform protections added by the Affordable Care Act. PMID:24611209

  18. 76 FR 72915 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DoD...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DoD Acquisition Process (Various Miscellaneous Requirements) (OMB Control Number 0704-0187) AGENCY: Department of Defense (DoD... Paperwork Reduction Act of 1995 (44 U.S.C. chapter 35), DoD announces the proposed extension of a...

  19. 77 FR 57199 - 2012 Real Estate Settlement Procedures Act (Regulation X) Mortgage Servicing Proposal

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ...The Bureau of Consumer Financial Protection (the Bureau) is proposing to amend Regulation X, which implements the Real Estate Settlement Procedures Act of 1974 (RESPA) and the official interpretation of the regulation. The proposed amendments implement the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act) provisions regarding mortgage loan servicing. Specifically, this......

  20. Users guide to working in and around water: Regulation under British Columbia`s Water Act. Revised edition

    SciTech Connect

    1998-12-01

    This booklet has been published to help understand and comply with the Regulation under the British Columbia Water Act for works and changes in and about a stream. Works carried out under the Regulation are those that do not involve water diversion, may be completed within a short period of time, and have little environmental impact. Examples include installation or removal of culverts or docks, repair of dikes, beaver dam removal, and installation of drain tile outlets. Information is included on definitions of terms, scope of the Regulation, general conditions for projects, specific project requirements, notification requirements, and enforcement. A copy of the Regulation is included.

  1. Maintaining Student Records and Meeting Confidentiality Requirements under the Family Education Rights and Privacy Act (FERPA), Individuals with Disability Education Act (IDEA), Section 504 of the Rehabilitation Act (504). A Primer for Educators

    ERIC Educational Resources Information Center

    Copenhaver, John

    2006-01-01

    An important federal statute impacting student records is the Family Educational Rights and Privacy Act (FERPA), enacted to ensure student/parent access to education records and to limit disclosures to others for unauthorized purposes. FERPA Regulations set forth the basic federal records retention and destruction requirements. The records of…

  2. The Americans with Disabilities Act and Family and Medical Leave Act: Legal Requirements, Negotiations and Policy Considerations.

    ERIC Educational Resources Information Center

    Juengart, Laurie S.

    The Americans with Disabilities Act (ADA) and Family and Medical Leave Act (FMLA) are two major pieces of social legislation that impact private and public employers, including school districts. Public school employers must have thorough awareness of the legal requirements of both laws and must analyze the ways in which those requirements…

  3. 7 CFR 868.10 - Information about the Service, Act, and regulations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Information about the Service, Act, and regulations. 868.10 Section 868.10 Agriculture Regulations of the Department of Agriculture (Continued) GRAIN... § 868.10 Information about the Service, Act, and regulations. Information about the Service,...

  4. 75 FR 55663 - Requirements for Federal Funding Accountability and Transparency Act Implementation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-14

    ... required for Transparency Act reporting of subawards and executive compensation [75 FR 43165]. The Federal... Reduction Act [75 FR 43165]. The instructions will address whether reporting of reductions in subaward... required for Transparency Act reporting of subawards and executive compensation [75 FR 43165]....

  5. 71 FR 66448 - Prescription Drug Marketing Act Pedigree Requirements under 21 CFR Part 203 Compliance Policy...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2006-11-15

    ... 92N-0297), 1988N-0258 (Formerly 88N- 0258), 2006D-0226] Prescription Drug Marketing Act Pedigree... Marketing Act Pedigree Requirements Questions and Answers; Notice of Availability AGENCY: Food and Drug... ``Prescription Drug Marketing Act--Pedigree Requirements under 21 CFR Part 203'' (PDMA CPG). This CPG...

  6. 48 CFR 52.225-10 - Notice of Buy American Act Requirement-Construction Materials.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false Notice of Buy American Act... Provisions and Clauses 52.225-10 Notice of Buy American Act Requirement—Construction Materials. As prescribed in 25.1102(b)(1), insert the following provision: Notice of Buy American Act...

  7. 48 CFR 52.225-10 - Notice of Buy American Act Requirement-Construction Materials.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Notice of Buy American Act... Provisions and Clauses 52.225-10 Notice of Buy American Act Requirement—Construction Materials. As prescribed in 25.1102(b)(1), insert the following provision: Notice of Buy American Act...

  8. 48 CFR 52.225-10 - Notice of Buy American Act Requirement-Construction Materials.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 2 2013-10-01 2013-10-01 false Notice of Buy American Act... Provisions and Clauses 52.225-10 Notice of Buy American Act Requirement—Construction Materials. As prescribed in 25.1102(b)(1), insert the following provision: Notice of Buy American Act...

  9. 48 CFR 52.225-10 - Notice of Buy American Act Requirement-Construction Materials.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 2 2011-10-01 2011-10-01 false Notice of Buy American Act... Provisions and Clauses 52.225-10 Notice of Buy American Act Requirement—Construction Materials. As prescribed in 25.1102(b)(1), insert the following provision: Notice of Buy American Act...

  10. 47 CFR 1.990 - Citizenship and filing requirements under the Communications Act of 1934.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., as amended, 15 U.S.C. 78a et seq. (Exchange Act), and corresponding Exchange Act Rule 13d-1, 17 CFR... corresponding Exchange Act Rule 13d-1, 17 CFR 240.13d-1, or a substantially comparable foreign law or regulation... PRACTICE AND PROCEDURE Grants by Random Selection Wireless Radio Services Applications and...

  11. 47 CFR 1.990 - Citizenship and filing requirements under the Communications Act of 1934.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., as amended, 15 U.S.C. 78a et seq. (Exchange Act), and corresponding Exchange Act Rule 13d-1, 17 CFR... corresponding Exchange Act Rule 13d-1, 17 CFR 240.13d-1, or a substantially comparable foreign law or regulation... PRACTICE AND PROCEDURE Grants by Random Selection Wireless Radio Services Applications and...

  12. Safe Medical Devices Act: management guidance for hospital compliance with the new FDA requirements.

    PubMed

    Alder, H C

    1993-10-01

    The Safe Medical Devices Act of 1990 (Public Law 101-629) was signed by President George Bush almost three years ago on November 28, 1990. The law expanded the Food and Drug Administration's (FDA) authority to regulate medical devices and grew out of congressional concerns about the FDA's ability to quickly learn when a medical device caused an adverse patient event, and to ensure that hazardous devices are removed from hospitals and other health care facilities in a timely manner. The Safe Medical Devices Act is an extension of the Medical Device Amendments of 1976, which imposed production, distribution, and sales rules on medical device manufacturers. It gives the FDA the legal authority to directly regulate the use of medical devices in health care facilities. Among the Safe Medical Devices Act's provisions are specific requirements for hospitals, health professionals, and other users of medical devices to report patient incidents involving medical devices to the manufacturer and to the FDA if a device caused or contributed to a serious injury, death, or other "adverse experience." Adverse experiences are defined by the FDA to include concussions, fractures, burns, temporary paralysis, and temporary loss of sight, hearing, or smell. Hospitals have been required to comply with this provision of the law, called user reporting, since 1991. Hospitals are also required to participate in tracking certain medical devices whose failure could result in a serious adverse health outcome. The law requires distributors and manufacturers of specific devices to adopt a method for device tracking. Hospitals are required to cooperate with and provide device manufacturers with information about patients with permanently implantable devices and life-sustaining and life-supporting devices used outside device user facilities. The law also gives the FDA the authority to designate other devices subject to tracking if the agency determines such tracking is warranted to preserve the

  13. Requirements and impacts of the Federal Facility Compliance Act on the Department of Energy

    SciTech Connect

    Chang, L.; Tripp, S.C.

    1993-03-01

    The Federal Facilities Compliance Act (FFCA, the Act) was signed into law on October 6, 1992, primarily as a means of waiving sovereign immunity for federal facilities with respect to requirements under the Resource Conservation and Recovery Act. DOE`s implementation of the FFCA will have significant effects on current and future DOE waste management operations. DOE will need to rethink its strategy in the area of future compliance agreements to ensure commitments and deliverables are made consistent throughout the different DOE facilities. Several types of agreements that address mixed waste land disposal restriction (LDR) compliance have already been signed by both DOE and the regulators. These agreements are in place at the Hanford Reservation, the Savannah River Site, the Oak Ridge Reservation (Oak Ridge National Laboratory, K-25, Y-12), and the Paducah Gaseous Diffusion Plant. The Rocky Flats Agreement is now being renegotiated. Los Alamos National Laboratory, Sandia/Albuquerque National Laboratory, Lawrence Livermore National Laboratory, and Idaho National Engineering Laboratory agreements are in progress. Major components of the FFCA include provisions on: sovereign immunity waiver; cost reimbursements; mixed waste requirements, including inventory reports on mixed waste and treatment capacity and technologies; and plans for the development of treatment capacities and technologies. Each of these components is discussed within this paper.

  14. Implementation of the Methamphetamine Anti-Proliferation Act; thresholds for retailers and for distributors required to submit mail order reports; changes to mail order reporting requirements. Final rule.

    PubMed

    2003-10-01

    This regulation implements the new threshold requirements and mail order reporting requirements of the Methamphetamine Anti-Proliferation Act of 2000 (MAPA), which was enacted on October 17, 2000. DEA is amending its regulations to reduce the thresholds for pseudoephedrine and phenylpropanolamine for retail distributors and for distributors required to submit mail order reports. Also, DEA is amending its regulations to require mail order reports for certain export transactions. DEA is codifying exemptions from the mail order reporting requirements for certain distributions to nonregulated persons and certain export transactions. This rule is consistent with the intent of MAPA to prevent the diversion of drug products to the clandestine manufacture of methamphetamine and amphetamine, and simultaneously reduce the industry reporting burden. PMID:14535265

  15. Implementing the Affordable Care Act: Revisiting the ACA's Essential Health Benefits Requirements.

    PubMed

    Giovannelli, Justin; Lucia, Kevin W; Corlette, Sabrina

    2014-10-01

    The Affordable Care Act broadens and strengthens the health insurance benefits available to consumers by requiring insurers to provide coverage of a minimum set of medical services known as "essential health benefits." Federal officials implemented this reform using transitional policies that left many important decisions to the states, while pledging to reassess that approach in time for the 2016 coverage year. This issue brief examines how states have exercised their options under the initial federal essential health benefits framework. We find significant variation in how states have developed their essential health benefits packages, including their approaches to benefit substitution and coverage of habilitative services. Federal regulators should use insurance company data describing enrollees' experiences with their coverage--information called for under the law's delayed transparency requirements--to determine whether states' differing strategies are producing the coverage improvements promised by reform. PMID:26259257

  16. 47 CFR 80.309 - Watch required by the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Safety Watches § 80.309 Watch required by the Bridge-to-Bridge Act. In addition to the watch requirement... 47 Telecommunication 5 2013-10-01 2013-10-01 false Watch required by the Bridge-to-Bridge Act. 80.309 Section 80.309 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND...

  17. 47 CFR 80.163 - Operator requirements of the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 5 2013-10-01 2013-10-01 false Operator requirements of the Bridge-to-Bridge Act. 80.163 Section 80.163 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND... Requirements § 80.163 Operator requirements of the Bridge-to-Bridge Act. Each ship subject to the...

  18. 77 FR 51784 - Federal Acquisition Regulation; Information Collection; Qualification Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-27

    ... minutes per submission assuming an offeror's use of electronic information tracking and retrieval... Regulation; Information Collection; Qualification Requirements AGENCY: Department of Defense (DOD), General... reinstatement request for an information collection requirement regarding an existing OMB clearance....

  19. 78 FR 30739 - Amendments to the 2013 Escrows Final Rule under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    .... \\1\\ 78 FR 4726 (Jan. 22, 2013). \\2\\ The other rules include: Ability-to-Repay and Qualified Mortgage Standards under the Truth in Lending Act (Regulation Z) (2013 ATR Final Rule), 78 FR 6407 (Jan. 30, 2013...) (2013 HOEPA Final Rule), 78 FR 6855 (Jan. 31, 2013); Disclosure and Delivery Requirements for Copies...

  20. 60 FR 8812 - Real Estate Settlement Procedures Act (Regulation X); Escrow Accounting Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    1995-02-15

    ... 24 CFR Part 3500 Real Estate Settlement Procedures Act (Regulation X); Escrow Accounting Procedures...-Federal Housing Commissioner 24 CFR Part 3500 RIN 2502-AF77 Real Estate Settlement Procedures Act... of the Real Estate Settlement Procedures Act. Subsequent to the publication of that rule,...

  1. 76 FR 68690 - Rules and Regulations Under the Textile Fiber Products Identification Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-07

    ... Wool Products Labeling Act, and the Fur Products Labeling Act: Final Rule, 63 FR 7508 (Feb. 13, 1998). \\2\\ Federal Trade Commission: Miscellaneous Rules: Final Rule, 63 FR 71582 (Dec. 29, 1998). \\3... and Regulations Under the Wool Products Labeling Act of 1939, Final Rule, 65 FR 75154 (Dec. 1,...

  2. 22 CFR 91.1 - Answering inquiries regarding tariff acts and customs regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Answering inquiries regarding tariff acts and... IMPORT CONTROLS § 91.1 Answering inquiries regarding tariff acts and customs regulations. In replying to inquiries received from exporters, travelers, or other interested parties, concerning tariff acts or...

  3. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Purpose, scope, and applicability of the Job... ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and applicability of the Job Training Partnership Act regulations. (a) Parts...

  4. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Purpose, scope, and applicability of the Job Training Partnership Act regulations. 626.3 Section 626.3 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and...

  5. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Purpose, scope, and applicability of the Job... ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and applicability of the Job Training Partnership Act regulations. (a) Parts...

  6. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Compliance with the Consumer Credit Protection Act and regulations. 374.3 Section 374.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) SPECIAL REGULATIONS IMPLEMENTATION OF THE CONSUMER CREDIT PROTECTION ACT WITH RESPECT TO AIR CARRIERS...

  7. 29 CFR 1903.2 - Posting of notice; availability of the Act, regulations and applicable standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 5 2010-07-01 2010-07-01 false Posting of notice; availability of the Act, regulations and... § 1903.2 Posting of notice; availability of the Act, regulations and applicable standards. (a)(1) Each... large type, generally not less than 36 pt. (b) Establishment means a single physical location...

  8. 78 FR 29263 - Rules andRegulations Under the Textile Fiber Products Identification Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-20

    ... Under the Textile Fiber Products Identification Act, 76 FR 68690 (Nov. 7, 2011). This Notice of Proposed...--Rules and Regulations Under the Textile Fiber Products Identification Act, 24 FR 4480, 4485 (June 2... Rulemaking, 50 FR 15100 at 15101 (Apr. 15, 1985). This Notice compared the Customs regulations in 19 CFR...

  9. 75 FR 50718 - Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-17

    ...This document contains proposed regulations implementing amendments to the Longshore and Harbor Workers' Compensation Act (LHWCA) by the American Recovery and Reinvestment Act of 2009 (ARRA), relating to the exclusion of certain recreational-vessel workers from the LHWCA's definition of ``employee.'' These regulations would clarify both the definition of ``recreational vessel'' and those......

  10. 77 FR 29982 - Federal Acquisition Regulation; Submission for OMB Review; Davis Bacon Act-Price Adjustment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... (actual method). A notice was published in the Federal Register at 77 FR 13328, on March 6, 2012. No... Regulation; Submission for OMB Review; Davis Bacon Act-Price Adjustment (Actual Method) AGENCY: Department of... Bacon Act-Price Adjustment (Actual Method), by any of the following methods: Regulations.gov :...

  11. 78 FR 73104 - Federal Travel Regulation (FTR); Agency Requirements for Payment of Expenses Connected With the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-05

    ... Heroes Act,'' dated September 12, 2011, and published in the Federal Register (76 FR 57621), delegates to...] RIN 3090-AJ37 Federal Travel Regulation (FTR); Agency Requirements for Payment of Expenses Connected.... General Services Administration (GSA). ACTION: Final rule. ] SUMMARY: GSA is amending the Federal...

  12. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly.

    PubMed

    Lettice, Laura A; Horikoshi, Taizo; Heaney, Simon J H; van Baren, Marijke J; van der Linde, Herma C; Breedveld, Guido J; Joosse, Marijke; Akarsu, Nurten; Oostra, Ben A; Endo, Naoto; Shibata, Minoru; Suzuki, Mikio; Takahashi, Eiichi; Shinka, Toshikatsu; Nakahori, Yutaka; Ayusawa, Dai; Nakabayashi, Kazuhiko; Scherer, Stephen W; Heutink, Peter; Hill, Robert E; Noji, Sumihare

    2002-05-28

    Preaxial polydactyly (PPD) is a common limb malformation in human. A number of polydactylous mouse mutants indicate that misexpression of Shh is a common requirement for generating extra digits. Here we identify a translocation breakpoint in a PPD patient and a transgenic insertion site in the polydactylous mouse mutant sasquatch (Ssq). The genetic lesions in both lie within the same respective intron of the LMBR1/Lmbr1 gene, which resides approximately 1 Mb away from Shh. Genetic analysis of Ssq reveals that the Lmbr1 gene is incidental to the phenotype and that the mutation directly interrupts a cis-acting regulator of Shh. This regulator is most likely the target for generating PPD mutations in human. PMID:12032320

  13. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly

    PubMed Central

    Lettice, Laura A.; Horikoshi, Taizo; Heaney, Simon J. H.; van Baren, Marijke J.; van der Linde, Herma C.; Breedveld, Guido J.; Joosse, Marijke; Akarsu, Nurten; Oostra, Ben A.; Endo, Naoto; Shibata, Minoru; Suzuki, Mikio; Takahashi, Eiichi; Shinka, Toshikatsu; Nakahori, Yutaka; Ayusawa, Dai; Nakabayashi, Kazuhiko; Scherer, Stephen W.; Heutink, Peter; Hill, Robert E.; Noji, Sumihare

    2002-01-01

    Preaxial polydactyly (PPD) is a common limb malformation in human. A number of polydactylous mouse mutants indicate that misexpression of Shh is a common requirement for generating extra digits. Here we identify a translocation breakpoint in a PPD patient and a transgenic insertion site in the polydactylous mouse mutant sasquatch (Ssq). The genetic lesions in both lie within the same respective intron of the LMBR1/Lmbr1 gene, which resides ≈1 Mb away from Shh. Genetic analysis of Ssq reveals that the Lmbr1 gene is incidental to the phenotype and that the mutation directly interrupts a cis-acting regulator of Shh. This regulator is most likely the target for generating PPD mutations in human. PMID:12032320

  14. 75 FR 75692 - Manufactured Home Construction and Safety Standards Act Reporting Requirements; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-06

    ... URBAN DEVELOPMENT Manufactured Home Construction and Safety Standards Act Reporting Requirements; Notice.... This Notice also lists the following information: Title of Proposal: Manufactured Home Construction and Safety Standards Reporting Requirements. OMB Control Number, if applicable: 2502-0253. Description of...

  15. 12 CFR 335.601 - Requirements of section 16 of the Securities Exchange Act of 1934.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... regulations issued under section 16 of the Exchange Act (17 CFR part 240), except that the forms described in... FDICconnect at https://www2.fdicconnect.gov/index.asp. Copies of FDIC Forms 3, 4, and 5 and the...

  16. 12 CFR 335.601 - Requirements of section 16 of the Securities Exchange Act of 1934.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... regulations issued under section 16 of the Exchange Act (17 CFR part 240), except that the forms described in... FDICconnect at https://www2.fdicconnect.gov/index.asp. Copies of FDIC Forms 3, 4, and 5 and the...

  17. 12 CFR 335.601 - Requirements of section 16 of the Securities Exchange Act of 1934.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... regulations issued under section 16 of the Exchange Act (17 CFR part 240), except that the forms described in... FDICconnect at https://www2.fdicconnect.gov/index.asp. Copies of FDIC Forms 3, 4, and 5 and the...

  18. 12 CFR 335.601 - Requirements of section 16 of the Securities Exchange Act of 1934.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... regulations issued under section 16 of the Exchange Act (17 CFR part 240), except that the forms described in... FDICconnect at https://www2.fdicconnect.gov/index.asp. Copies of FDIC Forms 3, 4, and 5 and the...

  19. 77 FR 13617 - Agency Information Collection Activities: Customs Modernization Act Recordkeeping Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-07

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Customs Modernization Act Recordkeeping Requirements AGENCY: U.S. Customs and Border Protection (CBP), Department of... concerning the Customs Modernization Act Recordkeeping Requirements. This request for comment is being...

  20. 75 FR 44893 - Pipeline Posting Requirements Under Section 23 of the Natural Gas Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-30

    .... 720, 73 FR 73,494 (Dec. 2, 2008), FERC Stats. & Regs. ] 31,283 (2008) (Order No. 720). \\2\\ Pipeline Posting Requirements under Section 23 of the Natural Gas Act, Order No. 720-A, 75 FR 5178 (Jan. 21, 2010... Requirements under Section 23 of the Natural Gas Act, 73 FR 1116 (Jan. 7, 2008), FERC Stats. & Regs....

  1. 45 CFR 73.735-901 - Reporting requirement of the Ethics in Government Act of 1978.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Reporting requirement of the Ethics in Government... requirement of the Ethics in Government Act of 1978. (a) Applicability. The following employees and special... Title II of the Ethics in Government Act of 1978, Pub. L. 95-521, as amended: (1) Officers and...

  2. 45 CFR 73.735-901 - Reporting requirement of the Ethics in Government Act of 1978.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 1 2012-10-01 2012-10-01 false Reporting requirement of the Ethics in Government... requirement of the Ethics in Government Act of 1978. (a) Applicability. The following employees and special... Title II of the Ethics in Government Act of 1978, Pub. L. 95-521, as amended: (1) Officers and...

  3. 45 CFR 73.735-901 - Reporting requirement of the Ethics in Government Act of 1978.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 1 2011-10-01 2011-10-01 false Reporting requirement of the Ethics in Government... requirement of the Ethics in Government Act of 1978. (a) Applicability. The following employees and special... Title II of the Ethics in Government Act of 1978, Pub. L. 95-521, as amended: (1) Officers and...

  4. 45 CFR 73.735-901 - Reporting requirement of the Ethics in Government Act of 1978.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 1 2013-10-01 2013-10-01 false Reporting requirement of the Ethics in Government... requirement of the Ethics in Government Act of 1978. (a) Applicability. The following employees and special... Title II of the Ethics in Government Act of 1978, Pub. L. 95-521, as amended: (1) Officers and...

  5. 45 CFR 73.735-901 - Reporting requirement of the Ethics in Government Act of 1978.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 1 2014-10-01 2014-10-01 false Reporting requirement of the Ethics in Government... requirement of the Ethics in Government Act of 1978. (a) Applicability. The following employees and special... Title II of the Ethics in Government Act of 1978, Pub. L. 95-521, as amended: (1) Officers and...

  6. Some impacts of the 1990 Clean Air Act and state clean-air regulations on the fertilizer industry

    SciTech Connect

    Breed, C.E.; Kerns, O.S.

    1992-12-01

    The Clean Air Act amendments of 1990 will intensify national efforts to reduce air pollution. They will have major impacts an governmental agencies and on industrial and commercial facilities throughout the country. As with other industries, it is essential for fertilizer dealers and producers to understand how these changes to the Clean Air Act can significantly change the way they do business. This paper is proffered as an overview of ways in which the 1990 amendments to the Clean Air Act may impact the fertilizer industry. The nonattainment, toxics, and permit provisions of the amended act will be three areas of particular concern to the fertilizer industry. Implementation of the new regulatory requirements of this legislation promises to be a long and onerous process for all concerned. However, it appears that state and local regulations may have a much more profound impact on the fertilizer industry than the new Clean Air Act.

  7. Some impacts of the 1990 Clean Air Act and state clean-air regulations on the fertilizer industry

    SciTech Connect

    Breed, C.E.; Kerns, O.S.

    1992-12-31

    The Clean Air Act amendments of 1990 will intensify national efforts to reduce air pollution. They will have major impacts on governmental agencies and on industrial and commercial facilities throughout the country. As with other industries, it is essential for fertilizer dealers and producers to understand how these changes to the Clean Air Act can significantly change the way they do business. This paper is proffered as an overview of ways in which the 1990 amendments to the Clean Air Act may impact the fertilizer industry. The nonattainment, toxics, and permit provisions of the amended act will be three areas of particular concern to the fertilizer industry. Implementation of the new regulatory requirements of this legislation promises to be a long and onerous process for all concerned. However, it appears that state and local regulations may have a much more profound impact on the fertilizer industry than the new Clean Air Act.

  8. MEETING THE REQUIREMENTS OF THE U.S. SAFE DRINKING WATER ACT: THE ROLE OF TECHNOLOGY

    EPA Science Inventory

    The passage of the U.S. Safe Drinking Water Act (SDWA) in 1974 has had a major impact on the way water is treated and delivered in the United States. The Act established national drinking water regulations for more than 170,000 public drinking water systems serving over 250 mill...

  9. 24 CFR 1000.42 - Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Development Act of 1968 (12 U.S.C. 1701u) and HUD's implementing regulations in 24 CFR part 135, to the... 24 Housing and Urban Development 4 2014-04-01 2014-04-01 false Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable? 1000.42 Section 1000.42 Housing...

  10. 24 CFR 1000.42 - Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Development Act of 1968 (12 U.S.C. 1701u) and HUD's implementing regulations in 24 CFR part 135, to the... 24 Housing and Urban Development 4 2013-04-01 2013-04-01 false Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable? 1000.42 Section 1000.42 Housing...

  11. 24 CFR 1000.42 - Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Development Act of 1968 (12 U.S.C. 1701u) and HUD's implementing regulations in 24 CFR part 135, to the... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable? 1000.42 Section 1000.42 Housing...

  12. 24 CFR 1000.42 - Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Development Act of 1968 (12 U.S.C. 1701u) and HUD's implementing regulations in 24 CFR part 135, to the... 24 Housing and Urban Development 4 2011-04-01 2011-04-01 false Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable? 1000.42 Section 1000.42 Housing...

  13. 24 CFR 1000.42 - Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Development Act of 1968 (12 U.S.C. 1701u) and HUD's implementing regulations in 24 CFR part 135, to the... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Are the requirements of section 3 of the Housing and Urban Development Act of 1968 applicable? 1000.42 Section 1000.42 Housing...

  14. 77 FR 66539 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ... electronic records and automated searches along with paper records and manual searches. In addition, FMCS's... twenty cents per page, while document search and review charges will increase to $4.00 per each quarter... revisions read as follows: Sec. 1401.36 Freedom of Information Act fee schedules. (a) * * * (2) Search...

  15. The Equal Access Act: Policy and Regulations for Implementation.

    ERIC Educational Resources Information Center

    Jones, H. Lawrence; Martin, James

    The Equal Access Act of 1984 was drafted to implement access for religious clubs to public secondary schools, but with constitutionally protected safeguards to preclude entanglement of church and state. A number of challenges were made on the constitutional grounds of excessive entanglement of church and state, as well as what the definition of…

  16. 77 FR 50390 - Equal Credit Opportunity Act (Regulation B)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... from the Bureau's rulemaking authority by section 1029 of the Dodd-Frank Act). 76 FR 79442 (Dec. 21... burdens on industry. Thus, many of the rulemakings listed above involve issues that extend across two or... Bureau planned to conduct qualitative testing with industry participants as well as consumers. Each...

  17. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2012-04-01 2011-04-01 true Federal Reserve Regulation Z and Fair Credit Reporting Act... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  18. The Final Regulations to the Carl D. Perkins Vocational and Applied Technology Education Act of 1990.

    ERIC Educational Resources Information Center

    American Vocational Association, Alexandria, VA.

    This publication presents the final regulations to the Carl D. Perkins Vocational and Applied Technology Education Act of 1990. The first section is an analysis of the regulations. This analysis compares the proposed regulations to the final version and explains the differences between the two and the potential impact on program administration.…

  19. 78 FR 54252 - Information Collection; American Recovery and Reinvestment Act-Reporting Requirements-One Time...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-03

    ... FR 58389, for the number of respondents required to comply with the requirements of FAR subpart 4.15... Register on September 24, 2010, at 75 FR 58389. No public comments were received in prior years that have...; American Recovery and Reinvestment Act-- Reporting Requirements--One Time Reporting Requirements for...

  20. 47 CFR 80.163 - Operator requirements of the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Operator requirements of the Bridge-to-Bridge Act. 80.163 Section 80.163 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Operator Requirements Ship Station Operator Requirements § 80.163 Operator requirements...

  1. 47 CFR 80.163 - Operator requirements of the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false Operator requirements of the Bridge-to-Bridge Act. 80.163 Section 80.163 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Operator Requirements Ship Station Operator Requirements § 80.163 Operator requirements...

  2. 47 CFR 80.163 - Operator requirements of the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 5 2012-10-01 2012-10-01 false Operator requirements of the Bridge-to-Bridge Act. 80.163 Section 80.163 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Operator Requirements Ship Station Operator Requirements § 80.163 Operator requirements...

  3. 47 CFR 80.163 - Operator requirements of the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Operator requirements of the Bridge-to-Bridge Act. 80.163 Section 80.163 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Operator Requirements Ship Station Operator Requirements § 80.163 Operator requirements...

  4. 77 FR 12754 - Contractor Legal Management Requirements; Acquisition Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-02

    ... Energy Acquisition Regulation (DEAR) (76 FR 81408). The comment period is reopened until March 16, 2012... published a NOPR in the Federal Register (76 FR 81408) to revise existing regulations covering contractor... Part 719 48 Parts 931, 952 and 970 RIN 1990-AA37 Contractor Legal Management Requirements;...

  5. 77 FR 76898 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ... Federal Register (77 FR 56592) and requested comments over a 60-day period ending on November 13, 2012... regulations must be based on the best available science and that the rulemaking process must allow for public.... Finally, the revisions include current cost figures to be used in calculating and charging fees...

  6. Auxin acts independently of DELLA proteins in regulating gibberellin levels.

    PubMed

    Reid, James B; Davidson, Sandra E; Ross, John J

    2011-03-01

    Shoot elongation is a vital process for plant development and productivity, in both ecological and economic contexts. Auxin and bioactive gibberellins (GAs), such as GA1, play critical roles in the control of elongation, along with environmental and endogenous factors, including other hormones such as the brassinosteroids. The effect of auxins, such as indole-3-acetic acid (IAA), is at least in part mediated by its effect on GA metabolism, since auxin up-regulates biosynthesis genes such as GA 3-oxidase and GA 20-oxidase and down regulates GA catabolism genes such as GA 2-oxidases, leading to elevated levels of bioactive GA 1. In our recent paper, we have provided evidence that this action of IAA is largely independent of DELLA proteins, the negative regulators of GA action, since the auxin effects are still present in the DELLA-deficient la cry-s genotype of pea. This was a crucial issue to resolve, since like auxin, the DELLAs also promote GA 1 synthesis and inhibit its deactivation. DELLAs are deactivated by GA, and thereby mediate a feedback system by which bioactive GA regulates its own level. However, our recent results, in themselves, do not show the generality of the auxin-GA relationship across species and phylogenetic groups or across different tissue types and responses. Further, they do not touch on the ecological benefits of the auxin-GA interaction. These issues are discussed below as well as the need for the development of suitable experimental systems to allow this process to be examined. PMID:21358281

  7. TRF2 acts as a transcriptional regulator in tumor angiogenesis

    PubMed Central

    Maï, Mounir El; Wagner, Kay-Dietrich; Michiels, Jean-François; Gilson, Eric; Wagner, Nicole

    2015-01-01

    We recently showed that telomeric repeat-binding factor 2 (TRF2) regulates gene expression to promote angiogenesis. We found that TRF2 is highly expressed in tumor vessels and transcriptionally activates platelet-derived growth factor receptor β to promote endothelial cell angiogenic properties independently of its function in telomere protection. This work identifies TRF2 as a promising dual target for cancer therapy. PMID:27308469

  8. 20 CFR 626.2 - Format of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., including complaint processing and compliance reviews, will be governed by the provisions of 29 CFR part 34... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Format of the Job Training Partnership Act... LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.2 Format of the...

  9. 20 CFR 626.4 - Table of contents for the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the Job Training Partnership Act, 20 CFR parts 626-638 and 1005, 1 is as follows: 1 Part 1005 was... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Table of contents for the Job Training..., DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.4 Table...

  10. 20 CFR 626.2 - Format of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., including complaint processing and compliance reviews, will be governed by the provisions of 29 CFR part 34... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Format of the Job Training Partnership Act... LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.2 Format of the...

  11. 20 CFR 626.4 - Table of contents for the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the Job Training Partnership Act, 20 CFR parts 626-638 and 1005, 1 is as follows: 1 Part 1005 was... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Table of contents for the Job Training..., DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.4 Table...

  12. The Fair Labor Standards Act and the Child Care Industry--What Regulations Apply.

    ERIC Educational Resources Information Center

    Lee, Elisa

    1996-01-01

    Discusses how the federal Fair Labor Standard Act (FLSA) affects the wages and work hours of child care employees and how FLSA interacts with analogous state laws. Examines how child care centers can determine whether FLSA regulations apply to them, existing exemptions to the current act, minimum wage and overtime, whether training time is working…

  13. 77 FR 13328 - Federal Acquisition Regulation; Information Collection; Davis Bacon Act-Price Adjustment (Actual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... Regulation; Information Collection; Davis Bacon Act--Price Adjustment (Actual Method) AGENCY: Department of... (actual method). Public comments are particularly invited on: Whether this collection of information is... comments identified by Information Collection 9000- 0154, Davis Bacon Act--Price Adjustment (Actual...

  14. 32 CFR 505.14 - Recordkeeping requirements under the Privacy Act.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Recordkeeping requirements under the Privacy Act. 505.14 Section 505.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS ARMY PRIVACY ACT PROGRAM § 505.14 Recordkeeping...

  15. 32 CFR 505.14 - Recordkeeping requirements under the Privacy Act.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Recordkeeping requirements under the Privacy Act. 505.14 Section 505.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS ARMY PRIVACY ACT PROGRAM § 505.14 Recordkeeping...

  16. atonal regulates neurite arborization but does not act as a proneural gene in the Drosophila brain

    NASA Technical Reports Server (NTRS)

    Hassan, B. A.; Bermingham, N. A.; He, Y.; Sun, Y.; Jan, Y. N.; Zoghbi, H. Y.; Bellen, H. J.

    2000-01-01

    Drosophila atonal (ato) is the proneural gene of the chordotonal organs (CHOs) in the peripheral nervous system (PNS) and the larval and adult photoreceptor organs. Here, we show that ato is expressed at multiple stages during the development of a lineage of central brain neurons that innervate the optic lobes and are required for eclosion. A novel fate mapping approach shows that ato is expressed in the embryonic precursors of these neurons and that its expression is reactivated in third instar larvae (L3). In contrast to its function in the PNS, ato does not act as a proneural gene in the embryonic brain. Instead, ato performs a novel function, regulating arborization during larval and pupal development by interacting with Notch.

  17. 47 CFR 80.309 - Watch required by the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Watch required by the Bridge-to-Bridge Act. 80.309 Section 80.309 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches § 80.309 Watch required by...

  18. 47 CFR 80.309 - Watch required by the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Watch required by the Bridge-to-Bridge Act. 80.309 Section 80.309 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches § 80.309 Watch required by...

  19. 47 CFR 80.309 - Watch required by the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 5 2012-10-01 2012-10-01 false Watch required by the Bridge-to-Bridge Act. 80.309 Section 80.309 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches § 80.309 Watch required by...

  20. 47 CFR 80.309 - Watch required by the Bridge-to-Bridge Act.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false Watch required by the Bridge-to-Bridge Act. 80.309 Section 80.309 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches § 80.309 Watch required by...

  1. Polycomb Group Protein Pcgf6 Acts as a Master Regulator to Maintain Embryonic Stem Cell Identity

    PubMed Central

    Yang, Chao-Shun; Chang, Kung-Yen; Dang, Jason; Rana, Tariq M.

    2016-01-01

    The polycomb repressive complex 1 (PRC1) is a multi-subunit complex that plays critical roles in the epigenetic modulation of gene expression. Here, we show that the PRC1 component polycomb group ring finger 6 (Pcgf6) is required to maintain embryonic stem cell (ESC) identity. In contrast to canonical PRC1, Pcgf6 acts as a positive regulator of transcription and binds predominantly to promoters bearing active chromatin marks. Pcgf6 is expressed at high levels in ESCs, and knockdown reduces the expression of the core ESC regulators Oct4, Sox2, and Nanog. Conversely, Pcgf6 overexpression prevents downregulation of these factors and impairs differentiation. In addition, Pcgf6 enhanced reprogramming in both mouse and human somatic cells. The genomic binding profile of Pcgf6 is highly similar to that of trithorax group proteins, but not of PRC1 or PRC2 complexes, suggesting that Pcgf6 functions atypically in ESCs. Our data reveal novel roles for Pcgf6 in directly regulating Oct4, Nanog, Sox2, and Lin28 expression to maintain ESC identity. PMID:27247273

  2. The NEPA mandate and federal regulation of the natural gas industry. [NEPA (National Environmental Policy Act)

    SciTech Connect

    Hoecker, J.J.

    1992-01-01

    Utility regulators increasingly take responsibility for the [open quotes]extemalities[close quotes] associated with their decisions, meaning the economic and social costs related to rate decisions or other kinds of authorizations. Yet, when Congress adopted the National Environmental Policy Act of 1969 (NEPA), it intervened to ensure protection of the natural environment, not from abuses by the citizenry but from the activities of the federal government itself. Comprised of action forcing procedures, NEPA was designed to infuse the decisional processes of federal agencies with a broad awareness of the environmental consequences of their actions. NEPA encourages decisionmakers to counterbalance the organic statutory and political missions of their departments or agencies with a sensitivity to the ecological consequences of their directives and authorizations. This paper examines how the requirements of NEPA have fared in the environment of classical public utility regulation at the Federal Energy Regulatory Commission. Commission proceedings did not evidence any widely held opinion that economic regulation of the gas industry is hostile to the NEPA process.

  3. 32 CFR 505.14 - Recordkeeping requirements under the Privacy Act.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... under the Privacy Act. (a) AR 25-400-2, The Army Records Information Management System (ARIMS). To maintain privacy records are required by the Army Records Information Management System (ARIMS) to...

  4. 32 CFR 505.14 - Recordkeeping requirements under the Privacy Act.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... under the Privacy Act. (a) AR 25-400-2, The Army Records Information Management System (ARIMS). To maintain privacy records are required by the Army Records Information Management System (ARIMS) to...

  5. 32 CFR 505.14 - Recordkeeping requirements under the Privacy Act.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... under the Privacy Act. (a) AR 25-400-2, The Army Records Information Management System (ARIMS). To maintain privacy records are required by the Army Records Information Management System (ARIMS) to...

  6. Cis-acting elements in its 3' UTR mediate post-transcriptional regulation of KRAS.

    PubMed

    Kim, Minlee; Kogan, Nicole; Slack, Frank J

    2016-03-15

    Multiple RNA-binding proteins and non-coding RNAs, such as microRNAs (miRNAs), are involved in post-transcriptional gene regulation through recognition motifs in the 3' untranslated region (UTR) of their target genes. The KRAS gene encodes a key signaling protein, and its messenger RNA (mRNA) contains an exceptionally long 3' UTR; this suggests that it may be subject to a highly complex set of regulatory processes. However, 3' UTR-dependent regulation of KRAS expression has not been explored in detail. Using extensive deletion and mutational analyses combined with luciferase reporter assays, we have identified inhibitory and stabilizing cis-acting regions within the KRAS 3' UTR that may interact with miRNAs and RNA-binding proteins, such as HuR. Particularly, we have identified an AU-rich 49-nt fragment in the KRAS 3' UTR that is required for KRAS 3' UTR reporter repression. This element contains a miR-185 complementary element, and we show that overexpression of miR-185 represses endogenous KRAS mRNA and protein in vitro. In addition, we have identified another 49-nt fragment that is required to promote KRAS 3' UTR reporter expression. These findings indicate that multiple cis-regulatory motifs in the 3' UTR of KRAS finely modulate its expression, and sequence alterations within a binding motif may disrupt the precise functions of trans-regulatory factors, potentially leading to aberrant KRAS expression. PMID:26930719

  7. MecA Protein Acts as a Negative Regulator of Genetic Competence in Streptococcus mutans

    PubMed Central

    Tian, Xiao-Lin; Dong, Gaofeng; Liu, Tianlei; Gomez, Zubelda A.; Wahl, Astrid; Hols, Pascal

    2013-01-01

    Streptococcus mutans develops competence for genetic transformation through a complex network that receives inputs from at least two signaling peptides, competence-stimulating peptide (CSP) and sigX-inducing peptide (XIP). The key step of competence induction is the transcriptional activation of comX, which encodes an alternative sigma factor, SigX (σX), controlling the expression of late competence genes essential for DNA uptake and recombination. In this study, we provide evidence that MecA acts as a negative regulator in the posttranslational regulation of SigX in S. mutans. Using luxAB transcriptional reporter strains, we demonstrate that MecA represses the expression of late competence genes in S. mutans grown in a complex medium that is subpermissive for competence induction by CSP. The negative regulation of competence by MecA requires the presence of a functional SigX. Accordingly, inactivation of MecA results in a prolonged competence state of S. mutans under this condition. We have also found that the AAA+ protease ClpC displays a similar repressing effect on late competence genes, suggesting that both MecA and ClpC function coordinately to regulate competence in the same regulatory circuit in S. mutans. This suggestion is strongly supported by the results of bacterial two-hybrid assays, which demonstrate that MecA interacts with both SigX and ClpC, forming a ternary SigX-MecA-ClpC complex. Western blot analysis also confirms that inactivation of MecA or ClpC results in the intracellular accumulation of the SigX in S. mutans. Together, our data support the notion that MecA mediates the formation of a ternary SigX-MecA-ClpC complex that sequesters SigX and thereby negatively regulates genetic competence in S. mutans. PMID:24039267

  8. 14-3-3 family members act coordinately to regulate mitotic progression.

    PubMed

    Dalal, Sorab N; Yaffe, Michael B; DeCaprio, James A

    2004-05-01

    The mitosis promoting phosphatase, cdc25C, is a target of both the DNA replication and DNA damage checkpoint pathways. These pathways regulate cdc25C function, in part, by promoting the association of cdc25C with 14-3-3 proteins, which results in the retention of cdc25C in the cytoplasm. To determine which 14-3-3 proteins were required to regulate cdc25C function, we tested the ability of various 14-3-3 family members to form a complex with and negatively regulate cdc25C in human cells. Two 14-3-3 family members, 14-3-3epsilon and 14-3-3gamma specifically formed a complex with cdc25C but not with the 14-3-3 binding defective cdc25C mutant, S216A. In addition, 14-3-3epsilon and 14-3-3gamma inhibited the ability of cdc25C, but not the S216A mutant, to induce premature chromatin condensation (PCC) in U-2OS cells. These results suggested that the reduction in PCC by 14-3-3epsilon and 14-3-3gamma was due to inhibition of cdc25C function. In contrast, 14-3-3sigma was unable to form a complex with cdc25C, but was able to inhibit the ability of both wild type cdc25C and S216A to induce PCC. This suggests that 14-3-3sigma regulates entry into mitosis independently of cdc25C and 14-3-3epsilon and 14-3-3gamma. Thus, specific members of the 14-3-3 family of proteins may act coordinately to maintain the DNA replication checkpoint by regulating the activity of different cell cycle proteins. PMID:15107609

  9. 78 FR 17176 - Federal Acquisition Regulation; Defense Base Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-20

    ... contractor and subcontractor responsibilities to obtain workers' compensation insurance or to qualify as a self-insurer, and other requirements, under the terms of the Longshore and Harbor Workers' Compensation... contractor and subcontractor responsibilities to obtain workers' compensation insurance or to qualify as...

  10. A novel cis-acting element required for DNA damage-inducible expression of yeast DIN7

    SciTech Connect

    Yoshitani, Ayako; Yoshida, Minoru; Ling Feng

    2008-01-04

    Din7 is a DNA damage-inducible mitochondrial nuclease that modulates the stability of mitochondrial DNA (mtDNA) in Saccharomyces cerevisiae. How DIN7 gene expression is regulated, however, has remained largely unclear. Using promoter sequence alignment, we found a highly conserved 19-bp sequence in the promoter regions of DIN7 and NTG1, which encodes an oxidative stress-inducible base-excision-repair enzyme. Deletion of the 19-bp sequence markedly reduced the hydroxyurea (HU)-enhanced DIN7 promoter activity. In addition, nuclear fractions prepared from HU-treated cells were used in in vitro band shift assays to reveal the presence of currently unidentified trans-acting factor(s) that preferentially bound to the 19-bp region. These results suggest that the 19-bp sequence is a novel cis-acting element that is required for the regulation of DIN7 expression in response to HU-induced DNA damage.

  11. 75 FR 58389 - Federal Acquisition Regulation; Information Collection; American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... Reporting Requirements for Prime Contractors AGENCY: Department of Defense (DOD), General Services... American Recovery and Reinvestment Act-Reporting Requirements--One Time Reporting Requirements for Prime... for which the burden is imposed on the prime contractor under the FAR requirements include...

  12. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  13. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  14. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  15. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  16. 75 FR 59609 - Rescission of Regulations Under the Comprehensive Smokeless Tobacco Health Education Act of 1986

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-28

    ... its smokeless tobacco regulations, 16 CFR Part 307, on November 4, 1986.\\1\\ 51 FR 40015. \\1\\ The... on utilitarian items. 56 FR 11654 (Mar. 20, 1991). II. Basis for Removal of Regulations On June 22... the enactment of the Family Smoking Prevention Act. 75 FR 3665 (Jan. 22, ] 2010). The...

  17. 78 FR 8963 - Update of Existing Privacy Act-NASA Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-07

    ... document contains corrections to the final regulations (NASA-2012-0005), which were published in the Federal Register of Thursday, October 4, 2012 (77 FR 60620). The regulations relate to requests to access... SPACE ADMINISTRATION 14 CFR Part 1212 RIN 2700-AD86 Update of Existing Privacy Act--NASA...

  18. GUAM - FACILITIES REGULATED UNDER THE RESOURCE CONVERVATION AND RECOVERY ACT (RCRA)

    EPA Science Inventory

    Points represent facilities that are regulated by the EPA under the Resource Conservation and Recovery Act (RCRA). Facilities regulated under RCRA generate, dispose of, treate or transport hazardous waste. RCRA is a law enacted by Congress in 1976 and amended in 1984 to include ...

  19. NEVADA FACILITIES REGULATED UNDER THE RESOURCE CONVERVATION AND RECOVERY ACT (RCRA)

    EPA Science Inventory

    Points represent facilities that are regulated by the EPA under the Resource Conservation and Recovery Act (RCRA). Facilities regulated under RCRA generate, dispose of, treate or transport hazardous waste. RCRA is a law enacted by Congress in 1976 and amended in 1984 to include ...

  20. 75 FR 53153 - Federal Acquisition Regulation; American Recovery and Reinvestment Act of 2009 (the Recovery Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-30

    ... Federal Register at 74 FR 14623, March 31, 2009. The public comment period ended June 1, 2009. As required... Register at 73 FR 43385, July 25, 2008). Tariff shift rules consider the Harmonized Tariff Schedule of the... domestic manufactured construction material'' (see Federal Register at 74 FR 14624, March 31, 2009)....

  1. The ACT of Enrollment: The College Enrollment Effects of State-Required College Entrance Exam Testing

    ERIC Educational Resources Information Center

    Klasik, Daniel

    2013-01-01

    Since 2001 Colorado, Illinois, and Maine have all enacted policies that require high school juniors to take college entrance exams--the SAT or the ACT. One goal of these policies was to increase college enrollment based on the belief that requiring students to take these exams would make students more likely to consider college as a viable option.…

  2. 47 CFR 80.305 - Watch requirements of the Communications Act and the Safety Convention.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and the Safety Convention. 80.305 Section 80.305 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches § 80.305 Watch requirements of the Communications Act and the...

  3. 47 CFR 80.305 - Watch requirements of the Communications Act and the Safety Convention.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Watch requirements of the Communications Act and the Safety Convention. 80.305 Section 80.305 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches...

  4. 47 CFR 80.305 - Watch requirements of the Communications Act and the Safety Convention.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Watch requirements of the Communications Act and the Safety Convention. 80.305 Section 80.305 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches...

  5. 47 CFR 80.305 - Watch requirements of the Communications Act and the Safety Convention.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false Watch requirements of the Communications Act and the Safety Convention. 80.305 Section 80.305 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches...

  6. 47 CFR 80.305 - Watch requirements of the Communications Act and the Safety Convention.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 5 2012-10-01 2012-10-01 false Watch requirements of the Communications Act and the Safety Convention. 80.305 Section 80.305 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Safety Watch Requirements and Procedures Ship Station Safety Watches...

  7. 76 FR 22095 - Clean Air Act: Opportunity To Comment, Activities Required by Federal Facilities Compliance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-20

    ...The Environmental Protection Agency (EPA) has entered into a federal facilities compliance agreement with the Tennessee Valley Authority (TVA), Docket No. CAA-04-2010-1760 (Compliance Agreement) to resolve alleged violations of the Clean Air Act and its implementing regulations at the eleven facilities that TVA owns and operates in Alabama, Kentucky, and Tennessee. EPA is hereby providing......

  8. 7 CFR 3575.40 - Equal opportunity and Fair Housing Act requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Federal Reserve Board Regulation, 12 CFR part 202.) (b) Fair Housing Act. Certain housing-related projects... Marketing Plan and compliance with the Housing and Urban Development accessibility guidelines except for... seq.). The lender will determine that the borrower has a valid plan in effect at all times....

  9. 7 CFR 3575.40 - Equal opportunity and Fair Housing Act requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Federal Reserve Board Regulation, 12 CFR part 202.) (b) Fair Housing Act. Certain housing-related projects... Marketing Plan and compliance with the Housing and Urban Development accessibility guidelines except for... seq.). The lender will determine that the borrower has a valid plan in effect at all times....

  10. 7 CFR 3575.40 - Equal opportunity and Fair Housing Act requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Federal Reserve Board Regulation, 12 CFR part 202.) (b) Fair Housing Act. Certain housing-related projects... Marketing Plan and compliance with the Housing and Urban Development accessibility guidelines except for... seq.). The lender will determine that the borrower has a valid plan in effect at all times....

  11. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis.

    PubMed

    Xu, Jie; Ding, Zhiwen; Vizcay-Barrena, Gema; Shi, Jianxin; Liang, Wanqi; Yuan, Zheng; Werck-Reichhart, Danièle; Schreiber, Lukas; Wilson, Zoe A; Zhang, Dabing

    2014-04-29

    Mature pollen is covered by durable cell walls, principally composed of sporopollenin, an evolutionary conserved, highly resilient, but not fully characterized, biopolymer of aliphatic and aromatic components. Here, we report that ABORTED MICROSPORES (AMS) acts as a master regulator coordinating pollen wall development and sporopollenin biosynthesis in Arabidopsis thaliana. Genome-wide coexpression analysis revealed 98 candidate genes with specific expression in the anther and 70 that showed reduced expression in ams. Among these 70 members, we showed that AMS can directly regulate 23 genes implicated in callose dissociation, fatty acids elongation, formation of phenolic compounds, and lipidic transport putatively involved in sporopollenin precursor synthesis. Consistently, ams mutants showed defective microspore release, a lack of sporopollenin deposition, and a dramatic reduction in total phenolic compounds and cutin monomers. The functional importance of the AMS pathway was further demonstrated by the observation of impaired pollen wall architecture in plant lines with reduced expression of several AMS targets: the abundant pollen coat protein extracellular lipases (EXL5 and EXL6), and CYP98A8 and CYP98A9, which are enzymes required for the production of phenolic precursors. These findings demonstrate the central role of AMS in coordinating sporopollenin biosynthesis and the secretion of materials for pollen wall patterning. PMID:24781116

  12. 40 CFR 63.312 - Existing regulations and requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission...

  13. 40 CFR 63.312 - Existing regulations and requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission...

  14. 40 CFR 63.312 - Existing regulations and requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission...

  15. 40 CFR 63.312 - Existing regulations and requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission...

  16. 40 CFR 63.312 - Existing regulations and requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission...

  17. Regulations for the administration and enforcement of the Radiation Control for Health and Safety Act of 1968

    SciTech Connect

    Not Available

    1988-04-01

    The purpose of the Act is to protect the public from unnecessary exposure to radiation from electronic products, including ionizing, nonionizing, electromagnetic, or particulate radiation, or any sonic, infrasonic, or ultrasonic wave. The regulations in the publication cover general provisions, statements of policy and interpretation, radiation protection recommendations, recordkeeping and reporting requirements for manufacturers, dealers, and distributors; notification requirements for defects or failure to comply; repurchase, repairs, or replacement of electronic products; importation of products; and performance standards for television receivers, cathode ray tubes, diagnostic x-ray equipment, radiographic equipment, fluoroscopic equipment, computer aided tomography equipment, microwave ovens, laser products, sunlamps, ultraviolet lamps, mercury lamps, and ultrasonic therapy products.

  18. Allowance trading under the Clean Air Act: Who should regulate, and when?

    SciTech Connect

    Lock, R.

    1993-07-01

    The goal of this paper is to explore how compliance with the Clean Air Act Amendments of 1990 (CAAA), especially Title IV and emission trading under it, will affect the current relationship between state and federal regulation. It is difficult, with the limited experience we have had under Title IV, to be definitive about or to be a very strong advocate of too many policy positions. What may be most helpful at this point is to identify where the difficult issues in state/federal relations might arise; and then to explore ways in which tensions might be either avoided or resolved. One anticipated conclusion is that a traditional regulatory mindset could be very destructive if applied to this new area of oversight without due sensitivity to what Congress is trying to achieve in Title IV. That concern pervaded the early legislative debates; and it persists today. Title IV presents some unique challenges to state regulators and will require some creative solutions and fresh thinking if the goals of Congress are to be realized and the full benefits that allowance trading can offer are to be reaped by electricity consumers. In the ultimate analysis, Title IV amounts to a massive internalization of the external costs imposed on society by acid rain deposition. (This places in serious question the notion of additional externality {open_quotes}adders{close_quotes} for sulfur dioxide (SO{sub 2}) and nitrous oxide (NO{sub x}) at the state level for utility supply planning purposes.) The whole point of Title IV is to give those directly charged with compliance, namely power producers, the maximum flexibility to pursue least-cost compliance solutions. Perhaps the biggest single factor in how well they do this will be how state regulators respond to their compliance and allowance trading initiatives.

  19. Cis-acting elements in its 3′ UTR mediate post-transcriptional regulation of KRAS

    PubMed Central

    Kim, Minlee; Kogan, Nicole; Slack, Frank J.

    2016-01-01

    Multiple RNA-binding proteins and non-coding RNAs, such as microRNAs (miRNAs), are involved in post-transcriptional gene regulation through recognition motifs in the 3′ untranslated region (UTR) of their target genes. The KRAS gene encodes a key signaling protein, and its messenger RNA (mRNA) contains an exceptionally long 3′ UTR; this suggests that it may be subject to a highly complex set of regulatory processes. However, 3′ UTR-dependent regulation of KRAS expression has not been explored in detail. Using extensive deletion and mutational analyses combined with luciferase reporter assays, we have identified inhibitory and stabilizing cis-acting regions within the KRAS 3′ UTR that may interact with miRNAs and RNA-binding proteins, such as HuR. Particularly, we have identified an AU-rich 49-nt fragment in the KRAS 3′ UTR that is required for KRAS 3′ UTR reporter repression. This element contains a miR-185 complementary element, and we show that overexpression of miR-185 represses endogenous KRAS mRNA and protein in vitro. In addition, we have identified another 49-nt fragment that is required to promote KRAS 3′ UTR reporter expression. These findings indicate that multiple cis-regulatory motifs in the 3′ UTR of KRAS finely modulate its expression, and sequence alterations within a binding motif may disrupt the precise functions of trans-regulatory factors, potentially leading to aberrant KRAS expression. PMID:26930719

  20. RCRA corrective action permit requirements and modifications under Subpart F regulations. RCRA Information Brief

    SciTech Connect

    Coalgate, J.

    1993-07-01

    The ground water protection requirements under the Resource Conservation and Recovery Act (RCRA), 40 CFR 264, Subpart F, apply to surface impoundments, waste plies, land treatment units, and landfills that received hazardous waste after July 26,1982 (i.e., regulated units). There are three phases to the Subpart F ground water protection requirements: detection monitoring, compliance monitoring, and corrective action. Subpart F corrective action applies to remediation of ground water contamination resulting from releases from regulated units at a treatment, storage, or disposal facility (TSDF). The TSDF owner or operator is responsible for complying with these requirements. This Information Brief provides information on the permit requirements under Subpart F. This Information Brief is one of a series on RCRA corrective action. The first step in the permitting process is for the facility to determine the need for ground-water monitoring. The regulations found in 40 CFR 264 Sections 264.90 to 264.100 (Subpart F) apply to all regulated units. A ``regulated unit`` is defined as a surface impoundment, waste pile, landfill, or land treatment unit that received hazardous waste after July 26, 1982. Such units require a permit under RCRA. Subpart F entails a three-phased program designed to detect, evaluate, and, if necessary, respond to ground water contamination. The ground-water protection standard, including identification of maximum contaminant levels (MCLs) under the Safe Drinking Water Act (SDWA) and alternate concentration limits (ACLs), is established with the permit application. Where MCLs and ACLs cannot be established, the standard may be established at background levels.

  1. 60 FR 11194 - Real Estate Settlement Procedures Act (Regulation X); Escrow Accounting Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    1995-03-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT Office of the Assistant Secretary for Housing-Federal Housing Commissioner 24 CFR Part 3500 RIN 2502-AF77 Real Estate Settlement Procedures Act (Regulation X); Escrow Accounting...

  2. 75 FR 40754 - Government in the Sunshine Act Regulations of the National Science Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-14

    ... From the Federal Register Online via the Government Publishing Office NATIONAL SCIENCE FOUNDATION 45 CFR Part 614 RIN 3145-AA53 Government in the Sunshine Act Regulations of the National Science Board AGENCY: National Science Board (NSB), National Science Foundation (NSF). ACTION: Direct final...

  3. The National Labor Relations Act and the Regulation of Public Employee Collective Bargaining

    ERIC Educational Resources Information Center

    McCann, Walter; Smiley, Stafford

    1976-01-01

    The arguments for and against federal assumption of the responsibility for regulating the relationship between public employers and public employees are analyzed. It is suggested that the National Labor Relations Act should be extended to include them, thereby imposing upon them a duty to bargain collectively. Available from: the Harvard…

  4. 77 FR 20295 - Regulations To Implement the Equal Employment Provisions of the Americans With Disabilities Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-04

    ... From the Federal Register Online via the Government Publishing Office ] EQUAL EMPLOYMENT OPPORTUNITY COMMISSION 29 CFR Part 1630 Regulations To Implement the Equal Employment Provisions of the Americans With Disabilities Act, as Amended CFR Correction In Title 29 of the Code of Federal...

  5. 78 FR 70515 - Petition To Promulgate Standards for Bears Under the Animal Welfare Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-26

    ...; ] DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service 9 CFR Part 3 Petition To Promulgate Standards for Bears Under the Animal Welfare Act Regulations AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Notice of petition. SUMMARY: We are notifying the public that the Animal and...

  6. 75 FR 24862 - Removing Regulations Implementing the Fish and Wildlife Conservation Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-06

    ...'' (59 FR 22951), E.O. 13175, and 512 DM 2. We have determined that there are no potential effects. This... Fish and Wildlife Service 50 CFR Part 83 RIN 1018-AX00 Removing Regulations Implementing the Fish and Wildlife Conservation Act AGENCY: Fish and Wildlife Service, Interior. ACTION: Proposed rule. SUMMARY:...

  7. 76 FR 57940 - Revision of Department of Justice Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-19

    ... NRPM published on March 21, 2011 (76 FR 15236), closed on April 20, 2011. This document reopens the... the Freedom of Information Act. The comment period for that rule closed on April 20, 2011. The... revising its existing regulations under the FOIA. (See 76 FR 15236.) The rule proposed to amend...

  8. 76 FR 35963 - Disclosure of Information; Privacy Act Regulations; Notice and Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ... CORPORATION 12 CFR Parts 309 and 310 RIN 3064-AD83 Disclosure of Information; Privacy Act Regulations; Notice... disclosure of information and the PA, as amended. In taking this action the FDIC's goal is to avoid potential... to correct addresses and other matters that have changed since the FDIC's rules involving...

  9. Impact of Clean Air Act Regulations on Nitrogen Fate and Transport in Neuse River Basin

    EPA Science Inventory

    This study investigated impacts of Clean Air Act Amendment (CAAA) NOx emissions regulations on the fate and transport of nitrogen for two watersheds in the Neuse River Basin, North Carolina, USA from 1990 to 2020. The Soil and Water Assessment Tool (SWAT) and the Community Multi-...

  10. 75 FR 14547 - Tribal Consultation on Draft Buy Indian Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Indian Affairs 48 CFR Chapter 14 RIN 1076-AE95 Tribal Consultation on Draft Buy Indian Act Regulations AGENCY: Bureau of Indian Affairs, Interior. ACTION: Notice of tribal consultation...

  11. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Compliance with the Consumer Credit... OF TRANSPORTATION (AVIATION PROCEEDINGS) SPECIAL REGULATIONS IMPLEMENTATION OF THE CONSUMER CREDIT PROTECTION ACT WITH RESPECT TO AIR CARRIERS AND FOREIGN AIR CARRIERS § 374.3 Compliance with the...

  12. 76 FR 20569 - Horse Protection Act; Petition for Amendments to Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... Animal and Plant Health Inspection Service 9 CFR Part 11 Horse Protection Act; Petition for Amendments to... changes to our horse protection regulations and our current enforcement practices and related policies... are noting, however, that certain requests in the petition lack authority in the Horse Protection...

  13. 41 CFR 301-10.136 - What exceptions to the Fly America Act requirements apply when I travel between the United States...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 4 2013-07-01 2012-07-01 true What exceptions to the Fly America Act requirements apply when I travel between the United States and another country? 301-10.136 Section 301-10.136 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES...

  14. Primer for Maintaining Accurate Special Education Records and Meeting Confidentiality Requirements When Serving Children with Disabilities--Family Educational Rights and Privacy Act (FERPA).

    ERIC Educational Resources Information Center

    Copenhaver, John

    This manual is intended to provide schools with guidance in complying with federal laws and regulations regarding record keeping and confidentiality in their services to students with disabilities. Part 1 focuses on requirements of the Family Education Rights and Privacy Act (FERPA), which ensures student/parent access to education records and…

  15. 15 CFR 781.4 - U.S. Government requests for information needed to satisfy the requirements of the APR or the Act.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false U.S. Government requests for information needed to satisfy the requirements of the APR or the Act. 781.4 Section 781.4 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND...

  16. 18 CFR Appendix A to Part 380 - Minimum Filing Requirements for Environmental Reports Under the Natural Gas Act

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Requirements for Environmental Reports Under the Natural Gas Act A Appendix A to Part 380 Conservation of Power... Filing Requirements for Environmental Reports Under the Natural Gas Act Environmental Reports Under the Natural Gas Act. Resource Report 1—General Project Description 1. Provide a detailed description...

  17. 18 CFR Appendix A to Part 380 - Minimum Filing Requirements for Environmental Reports Under the Natural Gas Act

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Requirements for Environmental Reports Under the Natural Gas Act A Appendix A to Part 380 Conservation of Power... Filing Requirements for Environmental Reports Under the Natural Gas Act Environmental Reports Under the Natural Gas Act. Resource Report 1—General Project Description 1. Provide a detailed description...

  18. 18 CFR Appendix A to Part 380 - Minimum Filing Requirements for Environmental Reports Under the Natural Gas Act

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Requirements for Environmental Reports Under the Natural Gas Act A Appendix A to Part 380 Conservation of Power... Filing Requirements for Environmental Reports Under the Natural Gas Act Environmental Reports Under the Natural Gas Act. Resource Report 1—General Project Description 1. Provide a detailed description...

  19. 2 CFR 176.50 - Award term-Reporting and registration requirements under section 1512 of the Recovery Act.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... requirements under section 1512 of the Recovery Act. 176.50 Section 176.50 Grants and Agreements Office of... INCLUDE FUNDS UNDER THE AMERICAN RECOVERY AND REINVESTMENT ACT OF 2009, PUBLIC LAW 111-5 Reporting and Registration Requirements Under Section 1512 of the American Recovery and Reinvestment Act of 2009 §...

  20. 2 CFR 176.50 - Award term-Reporting and registration requirements under section 1512 of the Recovery Act.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... requirements under section 1512 of the Recovery Act. 176.50 Section 176.50 Grants and Agreements Office of... INCLUDE FUNDS UNDER THE AMERICAN RECOVERY AND REINVESTMENT ACT OF 2009, PUBLIC LAW 111-5 Reporting and Registration Requirements Under Section 1512 of the American Recovery and Reinvestment Act of 2009 §...

  1. 2 CFR 176.50 - Award term-Reporting and registration requirements under section 1512 of the Recovery Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... requirements under section 1512 of the Recovery Act. 176.50 Section 176.50 Grants and Agreements Office of... AMERICAN RECOVERY AND REINVESTMENT ACT OF 2009, PUBLIC LAW 111-5 Reporting and Registration Requirements Under Section 1512 of the American Recovery and Reinvestment Act of 2009 § 176.50 Award...

  2. 2 CFR 176.50 - Award term-Reporting and registration requirements under section 1512 of the Recovery Act.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... requirements under section 1512 of the Recovery Act. 176.50 Section 176.50 Grants and Agreements Office of... THE AMERICAN RECOVERY AND REINVESTMENT ACT OF 2009, PUBLIC LAW 111-5 Reporting and Registration Requirements Under Section 1512 of the American Recovery and Reinvestment Act of 2009 § 176.50 Award...

  3. 48 CFR 352.237-72 - Crime Control Act-requirement for background checks.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... of 1990 (Act), requires that all individuals involved with the provision of child care services to children under the age of 18 undergo a criminal background check. “Child care services” include, but are not limited to, social services, health and mental health care, child (day) care, education...

  4. 77 FR 27787 - Agency Information Collection Activities: Customs Modernization Act Recordkeeping Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... collection was previously published in the Federal Register (77 FR 13617) on March 7, 2012, allowing for a 60... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Customs Modernization Act Recordkeeping Requirements AGENCY: U.S. Customs and Border Protection, Department of...

  5. 76 FR 71060 - Clarification of Duplication of Benefits Requirements Under the Stafford Act for Community...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ... URBAN DEVELOPMENT Clarification of Duplication of Benefits Requirements Under the Stafford Act for... (CDBG) disaster recovery grants, and all future CDBG disaster recovery grants. DATES: Effective Date... Issues Division, Office of Block Grant Assistance, Department of Housing and Urban Development, 451...

  6. EVALUATION OF A PROTOCOL FOR DRINKING WATER TREATMENT DATA REQUIRED BY THE FOOD QUALITY PROTECTION ACT

    EPA Science Inventory

    Under the Food Quality Protection Act (FQPA), the USEPA Office of Pesticide Programs (OPP) considers drinking water as a route for pesticide exposure in its human health risk assessments, and may require data on the fate of a pesticide in drinking water be supplied to OPP by the ...

  7. 47 CFR 20.15 - Requirements under Title II of the Communications Act.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... CFR part 68; and §§ 1.701-1.748, and 1.815 of this chapter, 47 CFR 1.701-1.748, 1.815. (b) Commercial... CARRIER SERVICES COMMERCIAL MOBILE RADIO SERVICES § 20.15 Requirements under Title II of the Communications Act. (a) Commercial mobile radio services providers, to the extent applicable, must comply...

  8. 49 CFR 27.19 - Compliance with Americans with Disabilities Act requirements and FTA policy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... General § 27.19 Compliance with Americans with Disabilities Act requirements and FTA policy. (a) Recipients subject to this part (whether public or private entities as defined in 49 CFR part 37) shall... 49 Transportation 1 2010-10-01 2010-10-01 false Compliance with Americans with Disabilities...

  9. 76 FR 33413 - Proposed Renewal Without Change; Comment Request; Nine Bank Secrecy Act Recordkeeping Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... international terrorism was added by section 358 of the Uniting and Strengthening America by Providing Appropriate Tools Required to Intercept and Obstruct Terrorism (USA PATRIOT) Act of 2001, Pubic Law 107-56... intelligence or counter-intelligence activities, including analysis, to protect against international...

  10. 78 FR 27001 - Horse Protection Act; Requiring Horse Industry Organizations To Assess and Enforce Minimum...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... published in the Federal Register on June 7, 2012 (77 FR 33607-33619, Docket No. APHIS-2011-0030), and... Inspection Service 9 CFR Part 11 RIN 0579-AD43 Horse Protection Act; Requiring Horse Industry Organizations... Federal Register on June 7, 2012, and effective on July 9, 2012, we amended the horse...

  11. 77 FR 33607 - Horse Protection Act; Requiring Horse Industry Organizations To Assess and Enforce Minimum...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... management. On May 27, 2011, we published in the Federal Register (76 FR 30864- 30868, Docket No. APHIS-2011... Animal and Plant Health Inspection Service 9 CFR Part 11 RIN 0579-AD43 Horse Protection Act; Requiring Horse Industry Organizations To Assess and Enforce Minimum Penalties for Violations AGENCY: Animal...

  12. 32 CFR 635.3 - Special requirements of the Privacy Act of 1974.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... DD Form 2 (Ret) (U.S. Armed Forces of the United States General Convention Identification Card), or... used. (c) Army law enforcement personnel performing official duties often require an individual's SSN... without violating an individual's privacy and without providing a Privacy Act Statement. This...

  13. 32 CFR 635.3 - Special requirements of the Privacy Act of 1974.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... DD Form 2 (Ret) (U.S. Armed Forces of the United States General Convention Identification Card), or... used. (c) Army law enforcement personnel performing official duties often require an individual's SSN... without violating an individual's privacy and without providing a Privacy Act Statement. This...

  14. 32 CFR 635.3 - Special requirements of the Privacy Act of 1974.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... DD Form 2 (Ret) (U.S. Armed Forces of the United States General Convention Identification Card), or... used. (c) Army law enforcement personnel performing official duties often require an individual's SSN... without violating an individual's privacy and without providing a Privacy Act Statement. This...

  15. 32 CFR 635.3 - Special requirements of the Privacy Act of 1974.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... DD Form 2 (Ret) (U.S. Armed Forces of the United States General Convention Identification Card), or... used. (c) Army law enforcement personnel performing official duties often require an individual's SSN... without violating an individual's privacy and without providing a Privacy Act Statement. This...

  16. 32 CFR 635.3 - Special requirements of the Privacy Act of 1974.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... DD Form 2 (Ret) (U.S. Armed Forces of the United States General Convention Identification Card), or... used. (c) Army law enforcement personnel performing official duties often require an individual's SSN... without violating an individual's privacy and without providing a Privacy Act Statement. This...

  17. 76 FR 26787 - Short Sale Reporting Study Required by Dodd-Frank Act Section 417(a)(2)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-09

    ...(b)(8). \\4\\ See 17 CFR 242.200(a). \\5\\ See Exchange Act Release No. 50103 (July 28, 2004), 69 FR...., Exchange Act Release No. 58775 (Oct. 14, 2008), 73 FR 61690 (Oct. 17, 2008). \\13\\ In adopting Regulation... short position is not net of long position.'' See Exchange Act Release No. 58785 (Oct. 15, 2008), 73...

  18. Rationing health protection: a proposal to exempt nuisance dust from US Clean Air Act regulations.

    PubMed

    Centner, Terence J; Colson, Gregory

    2013-03-15

    The US House of Representative has passed a bill called the "Farm Dust Regulation Prevention Act" (Dust Act) that would exempt most types of particulate matter (PM) in rural areas from the air quality controls of the US Clean Air Act. The Dust Act would markedly change the country's air quality standards. An examination of the proposed provisions shows that they would exempt non-combustion PM pollutants from mining, smelting, petroleum production, and power generation from existing air quality standards. Persons downwind from pollutants generated in rural areas could be exposed to concentrations of carcinogenic heavy metals, asbestos, and benzene known to adversely affect their health and ecological resources. Existing federal air quality standards based on science would be replaced by a flexible standard that rations health protection. PMID:23376304

  19. The Clean Air Act Amendments of 1990: Hazardous Air Pollutant Requirements and the DOE Clean Coal Technology Program

    SciTech Connect

    Moskowitz, P.D.; DePhillips, M.; Fthenakis, V.M. ); Hemenway, A. )

    1991-01-01

    The purpose of the US Department of Energy -- Office of Fossil Energy (DOE FE) Clean Coal Technology Program (CCTP) is to provide the US energy marketplace with advanced, efficient, and environmentally sound coal-based technologies. The design, construction, and operation of Clean Coal Technology Demonstration Projects (CCTDP) will generate data needed to make informed, confident decisions on the commercial readiness of these technologies. These data also will provide information needed to ensure a proactive response by DOE and its industrial partners to the establishment of new regulations or a reactive response to existing regulations promulgated by the US Environmental Protection Agency (EPA). The objectives of this paper are to: (1) Present a preliminary examination of the potential implications of the Clean Air Act Amendments (CAAA) -- Title 3 Hazardous Air Pollutant requirements to the commercialization of CCTDP; and (2) help define options available to DOE and its industrial partners to respond to this newly enacted Legislation.

  20. The Clean Air Act Amendments of 1990: Hazardous Air Pollutant Requirements and the DOE Clean Coal Technology Program

    SciTech Connect

    Moskowitz, P.D.; DePhillips, M.; Fthenakis, V.M.; Hemenway, A.

    1991-12-31

    The purpose of the US Department of Energy -- Office of Fossil Energy (DOE FE) Clean Coal Technology Program (CCTP) is to provide the US energy marketplace with advanced, efficient, and environmentally sound coal-based technologies. The design, construction, and operation of Clean Coal Technology Demonstration Projects (CCTDP) will generate data needed to make informed, confident decisions on the commercial readiness of these technologies. These data also will provide information needed to ensure a proactive response by DOE and its industrial partners to the establishment of new regulations or a reactive response to existing regulations promulgated by the US Environmental Protection Agency (EPA). The objectives of this paper are to: (1) Present a preliminary examination of the potential implications of the Clean Air Act Amendments (CAAA) -- Title 3 Hazardous Air Pollutant requirements to the commercialization of CCTDP; and (2) help define options available to DOE and its industrial partners to respond to this newly enacted Legislation.

  1. Review of orders and regulations requiring environmental protection

    SciTech Connect

    Kelly, E.; Cunningham, R.; Michael, D.

    1996-09-01

    With the increased awareness of and interest in potential ecological risks associated with past, current, and future Department of Energy (DOE) activities, DOE`s Defense Programs (DP) Office of Technical and Environmental Support sponsored a study to (1) evaluate the effectiveness of the current compliance-driven environmental protection and assessment efforts relative to ecological concerns; (2) explore the need for a more focused, integrated approach to address ecological impacts; and (3) identify the requirements for an integrated approach. The study explored four questions. (a) Which federal regulations and DOE orders either explicitly require ecological assessments or implicitly require them through environmental protection language? (b) What currently is being done at selected DOE facilities to implement these regulations and orders? (c) What are private sector industries doing in terms of ecological risk assessments and how do industry approaches and issues compare with those of DOE? (d) What, if anything, in addition to current efforts is needed to ensure the protection of ecological resources associated with DOE facilities, to support defensible decision making, and to improve efficiency? The results of this study are presented in a report titled {open_quotes}Integrated, Comprehensive Ecological Impact Assessments In Support of Department of Energy Decision Making{close_quotes}. This report is a companion document to that report. This report provides a more detailed discussion of the document reviews of the relevant environmental protection regulations and current and pending DOE orders. The main goal of the document reviews was to understand existing requirements for ecological data collection and impact assessments.

  2. Regulating the regulator: Numb acts upstream of p53 to control mammary stem and progenitor cell

    PubMed Central

    Faraldo, Marisa M.

    2015-01-01

    In this issue, Tosoni et al. (2015. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201505037) report that cell fate determinant and tumor suppressor Numb imposes asymmetric cell divisions in mammary stem cells by regulating p53. Numb thereby restricts mammary stem cell expansion and controls the proliferation and lineage-specific characteristics of their progeny. PMID:26598611

  3. Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4

    PubMed Central

    Foresti, Ombretta; Ruggiano, Annamaria; Hannibal-Bach, Hans K; Ejsing, Christer S; Carvalho, Pedro

    2013-01-01

    Sterol homeostasis is essential for the function of cellular membranes and requires feedback inhibition of HMGR, a rate-limiting enzyme of the mevalonate pathway. As HMGR acts at the beginning of the pathway, its regulation affects the synthesis of sterols and of other essential mevalonate-derived metabolites, such as ubiquinone or dolichol. Here, we describe a novel, evolutionarily conserved feedback system operating at a sterol-specific step of the mevalonate pathway. This involves the sterol-dependent degradation of squalene monooxygenase mediated by the yeast Doa10 or mammalian Teb4, a ubiquitin ligase implicated in a branch of the endoplasmic reticulum (ER)-associated protein degradation (ERAD) pathway. Since the other branch of ERAD is required for HMGR regulation, our results reveal a fundamental role for ERAD in sterol homeostasis, with the two branches of this pathway acting together to control sterol biosynthesis at different levels and thereby allowing independent regulation of multiple products of the mevalonate pathway. DOI: http://dx.doi.org/10.7554/eLife.00953.001 PMID:23898401

  4. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Are my HCT/P's regulated solely under section 361..., AND CELLULAR AND TISSUE-BASED PRODUCTS General Provisions § 1271.10 Are my HCT/P's regulated solely.../P is regulated solely under section 361 of the PHS Act and the regulations in this part if it...

  5. 75 FR 6186 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 205...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 205, Publicizing Contract Actions AGENCY: Defense Acquisition Regulations System... approved information collection requirement. SUMMARY: In compliance with Section 3506(c)(2)(A) of...

  6. 75 FR 12518 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 237...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 237, Service Contracting AGENCY: Defense Acquisition Regulations System... approved information collection requirement. SUMMARY: In compliance with Section 3506(c)(2)(A) of...

  7. 78 FR 15935 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-13

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS); Bonds and Insurance AGENCY: Defense Acquisition Regulations System, Department... approved information collection requirement. SUMMARY: In compliance with Section 3506(c)(2)(A) of...

  8. 76 FR 50461 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Types of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-15

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Types of Contracts AGENCY: Defense Acquisition Regulations System, Department of... information collection requirement. SUMMARY: In compliance with Section 3506(c)(2)(A) of the...

  9. 75 FR 20825 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DoD Pilot...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-21

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DoD Pilot Mentor-Protege Program AGENCY: Defense Acquisition Regulations System... approved information collection requirement. ] SUMMARY: In compliance with section 3506(c)(2)(A) of...

  10. 75 FR 20825 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 211...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-21

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 211, Describing Agency Needs AGENCY: Defense Acquisition Regulations System... approved information collection requirement. SUMMARY: In compliance with Section 3506(c)(2)(A) of...

  11. 78 FR 70025 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-22

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Subcontracting Policies and Procedures AGENCY: Defense Acquisition Regulations System... approved information collection requirement. SUMMARY: In compliance with section 3506(c)(2)(A) of...

  12. Industry invites regulation: the passage of the Pure Food and Drug Act of 1906.

    PubMed Central

    Barkan, I D

    1985-01-01

    Ending its 27-year stranglehold on proposals for federal pure food and drug legislation, Congress passed the Pure Food and Drug Act and its companion bill, the Meat Inspection Act, on June 30, 1906. An unprecedented convergence of consumer, scientific, and industrial support in 1906 prompted such action; most industries even planned for it, hoping regulation would restore the competitiveness of their products on weak foreign and domestic markets. The ways in which these interests converged, and the reasons therefore, suggest a change in their relationships to each other and with the federal government as America headed into the twentieth century. Images p21-a p21-b PMID:3881052

  13. 75 FR 17919 - Submission for OMB Review; American Recovery and Reinvestment Act-One-time Reporting Requirements...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-08

    ... collection requirement concerning the American Recovery and Reinvestment Act--One-time Reporting Requirements for Prime Contractors. A request for public comments was published in the Federal Register at 74 FR...; American Recovery and Reinvestment Act--One-time Reporting Requirements for Prime Contractors...

  14. The Maharashtra Felling of Trees (Regulation) (Amendment) Act, 1988 (No. 26 of 1989), 5 August 1989.

    PubMed

    1989-01-01

    Among other things, this Act amends the Maharashtra (India) Felling of Trees (Regulation) Act, 1964, to authorize the Tree Officer to order the planting of trees in any land (other than government-designated drought land) that he thinks does not contain an adequate number of trees. Owners and occupiers of the land must comply with the order, after being given a reasonable opportunity to be heard. If they do not comply, they can be charged for expenses incurred in having the trees planted. In 1989 the government of West Bengal enacted The Indian Forest (West Bengal Amendment) Act, 1989 (No. 22 of 1988) (Calcutta Gazette, Extraordinary, Part III, 3 February 1989). This Act increases the punishment for various infractions of the Indian Forest Act, 1927, as applied to West Bengal and inserts new language relating to the power of officers to stop and search vehicles; the ability of the state government to seize contraband and related tools, vehicles, boats, and cattle; and punishment for abetting offenses delineated by the Act. PMID:12344326

  15. 78 FR 45842 - Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedure Act (Regulation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ... From the Federal Register Online via the Government Publishing Office BUREAU OF CONSUMER FINANCIAL PROTECTION 12 CFR Parts 1024 and 1026 RIN 3170-AA37 Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedure Act (Regulation X) and the Truth in Lending Act (Regulation Z) Correction...

  16. 26 CFR 1.706-2T - Temporary regulations; question and answer under the Tax Reform Act of 1984.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Temporary regulations; question and answer under the Tax Reform Act of 1984. 1.706-2T Section 1.706-2T Internal Revenue INTERNAL REVENUE SERVICE... Partnerships § 1.706-2T Temporary regulations; question and answer under the Tax Reform Act of 1984. Question...

  17. 26 CFR 1.706-2T - Temporary regulations; question and answer under the Tax Reform Act of 1984.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Temporary regulations; question and answer under the Tax Reform Act of 1984. 1.706-2T Section 1.706-2T Internal Revenue INTERNAL REVENUE SERVICE...-2T Temporary regulations; question and answer under the Tax Reform Act of 1984. Question 1:...

  18. 26 CFR 18.0 - Effective date of temporary regulations under the Subchapter S Revision Act of 1982.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 14 2014-04-01 2013-04-01 true Effective date of temporary regulations under the Subchapter S Revision Act of 1982. 18.0 Section 18.0 Internal Revenue INTERNAL REVENUE SERVICE... SUBCHAPTER S REVISION ACT OF 1982 § 18.0 Effective date of temporary regulations under the Subchapter...

  19. 26 CFR 18.0 - Effective date of temporary regulations under the Subchapter S Revision Act of 1982.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 14 2012-04-01 2012-04-01 false Effective date of temporary regulations under the Subchapter S Revision Act of 1982. 18.0 Section 18.0 Internal Revenue INTERNAL REVENUE SERVICE... SUBCHAPTER S REVISION ACT OF 1982 § 18.0 Effective date of temporary regulations under the Subchapter...

  20. 76 FR 78483 - S.A.F.E. Mortgage Licensing Act (Regulations G & H)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    ... part of the public record and subject to public disclosure. Sensitive personal information, such as... for licensing loan originators that meets the requirements of the S.A.F.E. Act. \\1\\ 75 FR 44656 (July... this rule. See 76 FR 43569 (July 21, 2011). \\6\\ Public Law 111-203, section 1002(14)...

  1. 76 FR 63817 - Disclosure of Information; Privacy Act Regulations; Notice and Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-14

    ... supervisory agency, for purposes of Sec. 309.6, to which exempt records could be disclosed.\\1\\ \\1\\ 76 FR 35963.... D. Plain Language Section 722 of the Gramm-Leach-Bliley Act (Pub. L. 106-102, 113 Stat. 1338, 1471), requires the Federal banking agencies to use plain language in all proposed and final rules published...

  2. 48 CFR 952.226-72 - Energy Policy Act subcontracting goals and reporting requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of Education pursuant to 34 CFR 608.2; and (3) Small business concerns, as defined under section 3 of...) An institution of higher education that meets the requirements of 34 CFR 600.4(a), and has a student... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Energy Policy...

  3. 48 CFR 952.226-72 - Energy Policy Act subcontracting goals and reporting requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... of Education pursuant to 34 CFR 608.2; and (3) Small business concerns, as defined under section 3 of...) An institution of higher education that meets the requirements of 34 CFR 600.4(a), and has a student... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Energy Policy...

  4. 48 CFR 52.204-11 - American Recovery and Reinvestment Act-Reporting Requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false American Recovery and... Text of Provisions and Clauses 52.204-11 American Recovery and Reinvestment Act—Reporting Requirements. As prescribed in 4.1502, insert the following clause: American Recovery and Reinvestment...

  5. An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development.

    PubMed

    Chae, Eunyoung; Tan, Queenie K-G; Hill, Theresa A; Irish, Vivian F

    2008-04-01

    Plants flower in response to both environmental and endogenous signals. The Arabidopsis LEAFY (LFY) transcription factor is crucial in integrating these signals, and acts in part by activating the expression of multiple floral homeotic genes. LFY-dependent activation of the homeotic APETALA3 (AP3) gene requires the activity of UNUSUAL FLORAL ORGANS (UFO), an F-box component of an SCF ubiquitin ligase, yet how this regulation is effected has remained unclear. Here, we show that UFO physically interacts with LFY both in vitro and in vivo, and this interaction is necessary to recruit UFO to the AP3 promoter. Furthermore, a transcriptional repressor domain fused to UFO reduces endogenous LFY activity in plants, supporting the idea that UFO acts as part of a transcriptional complex at the AP3 promoter. Moreover, chemical or genetic disruption of proteasome activity compromises LFY-dependent AP3 activation, indicating that protein degradation is required to promote LFY activity. These results define an unexpected role for an F-box protein in functioning as a DNA-associated transcriptional co-factor in regulating floral homeotic gene expression. These results suggest a novel mechanism for promoting flower development via protein degradation and concomitant activation of the LFY transcription factor. This mechanism may be widely conserved, as homologs of UFO and LFY have been identified in a wide array of plant species. PMID:18287201

  6. Insulin/IGF-1 and Hypoxia Signaling Act in Concert to Regulate Iron Homeostasis in Caenorhabditis elegans

    PubMed Central

    Ackerman, Daniel; Gems, David

    2012-01-01

    Iron plays an essential role in many biological processes, but also catalyzes the formation of reactive oxygen species (ROS), which can cause molecular damage. Iron homeostasis is therefore a critical determinant of fitness. In Caenorhabditis elegans, insulin/IGF-1 signaling (IIS) promotes growth and reproduction but limits stress resistance and lifespan through inactivation of the DAF-16/FoxO transcription factor (TF). We report that long-lived daf-2 insulin/IGF-1 receptor mutants show a daf-16–dependent increase in expression of ftn-1, which encodes the iron storage protein H-ferritin. To better understand the regulation of iron homeostasis, we performed a TF–limited genetic screen for factors influencing ftn-1 gene expression. The screen identified the heat-shock TF hsf-1, the MAD bHLH TF mdl-1, and the putative histone acetyl transferase ada-2 as activators of ftn-1 expression. It also revealed that the HIFα homolog hif-1 and its binding partner aha-1 (HIFβ) are potent repressors of ftn-1 expression. ftn-1 expression is induced by exposure to iron, and we found that hif-1 was required for this induction. In addition, we found that the prolyl hydroxylase EGL-9, which represses HIF-1 via the von Hippel-Lindau tumor suppressor VHL-1, can also act antagonistically to VHL-1 in regulating ftn-1. This suggests a novel mechanism for HIF target gene regulation by these evolutionarily conserved and clinically important hydroxylases. Our findings imply that the IIS and HIF pathways act together to regulate iron homeostasis in C. elegans. We suggest that IIS/DAF-16 regulation of ftn-1 modulates a trade-off between growth and stress resistance, as elevated iron availability supports growth but also increases ROS production. PMID:22396654

  7. Energy codes and the Recovery Act: Guidance for states addressing 90% compliance requirements

    NASA Astrophysics Data System (ADS)

    Williams, Jeremiah Lee

    The American Recovery and Reinvestment Act of 2009 provided state financial support aimed at stimulating the national economy while creating efficiency gains through the implementation of modern building energy codes. These requirements call for compliance with targeted energy codes, including a plan to demonstrate a 90% compliance rate by 2017. In support of these requirements, the Federal Government has published a recommended methodology for states to reference as they work to demonstrate compliance with new energy codes. Unfortunately, the path to meeting this goal remains unclear and many questions still exist at the state and stakeholder levels. The present study examines compliance issues faced within U.S. states to enable effective policy decisions, with a primary goal to provide states with guidance and options to address energy code compliance requirements. A descriptive statistical analysis of survey responses gathered in code enforcement jurisdictions provides a baseline of local practices and current implementation issues. State challenges and subsequent recommendations are analyzed through recent case studies, and considered against expectations of the Federal methodology. Findings culminate in a set of recommendations with consideration for unique background scenarios and barriers observed in the study. The resulting guidance can be considered by states as they install policies and take action in addressing Recovery Act requirements.

  8. 75 FR 22805 - Submission for OMB Review; American Recovery and Reinvestment Act-One-Time Reporting Requirements...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ..., withdrawal. SUPPLEMENTARY INFORMATION: A. Background The Notice, published in the Federal Register at 75 FR...; American Recovery and Reinvestment Act--One-Time Reporting Requirements for Prime Contractors AGENCIES... Recovery and Reinvestment Act--One-time Reporting Requirements for Prime Contractors published in...

  9. Modeling Requirements for Simulating the Effects of Extreme Acts of Terrorism: A White Paper

    SciTech Connect

    Allen, M.; Hiebert-Dodd, K.; Marozas, D.; Paananen, O.; Pryor, R.J.; Reinert, R.K.

    1998-10-01

    This white paper presents the initial requirements for developing a new computer model for simulating the effects of extreme acts of terrorism in the United States. General characteristics of the model are proposed and the level of effort to prepare a complete written description of the model, prior to coding, is detailed. The model would simulate the decision processes and interactions of complex U. S. systems engaged in responding to and recovering from four types of terrorist incidents. The incident scenarios span the space of extreme acts of terrorism that have the potential to affect not only the impacted area, but also the entire nation. The model would be useful to decision-makers in assessing and analyzing the vulnerability of the nation's complex infrastructures, in prioritizing resources to reduce risk, and in planning strategies for immediate response and for subsequent recovery from terrorist incidents.

  10. 78 FR 44685 - Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedures Act (Regulation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-24

    ... Final Rule), 78 FR 6407 (Jan. 30, 2013). \\2\\ Mortgage Servicing Rules Under the Real Estate Settlement... Final Rules), 78 FR 10695 (Feb. 14, 2013) (Regulation X), 78 FR 10901 (Feb. 14, 2013) (Regulation Z). \\3\\ Amendments to the 2013 Escrows Final Rule under the Truth in Lending Act (Regulation Z), 78 FR 30739 (May...

  11. 78 FR 13298 - Notice of Retrospective Review of the Americans With Disabilities Act Regulations for Over-the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-27

    ... Statement in the Federal Register published on April 11, 2000 (65 FR 19477). FOR FURTHER INFORMATION CONTACT... Act Regulations for Over-the-Road Bus Operators; Request for Comments AGENCY: Office of the Secretary...) implementing regulations for over-the-road bus (OTRB) operators. The DOT will review regulations specified...

  12. Revision of Department of Justice Freedom of Information Act and Privacy Act Regulations and Implementation of Electronic Freedom of Information Act Amendments of 1996--DoJ. Proposed Rule.

    PubMed

    1997-08-26

    This document sets forth proposed revision of the Department's regulations under both the Freedom of Information Act (FOIA) and the Privacy Act of 1974. The FOIA and Privacy Act regulations have been streamlined and condensed, in accordance with the principles of the National Performance Reviews. With more "user-friendly" language wherever possible. These revisions also reflect the principles established by President Clinton and Attorney General Reno in their FOIA Policy Memoranda of October 4, 1993. The new statement of discretionary disclosure policy will supercede the existing regulation regarding discretionary access to records of historical interest. Additionally, the regulations have been updated to reflect developments in the case law and to include updated cost figures to be used in calculating and charging fees. These proposed revisions also contain new provisions implementing the Electronic Freedom of Information Act Amendments of 1996. PMID:10169840

  13. Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration

    PubMed Central

    Bernis, Cyril; Swift-Taylor, Beth; Nord, Matthew; Carmona, Sarah; Chook, Yuh Min; Forbes, Douglass J.

    2014-01-01

    The nuclear import receptors importin β and transportin play a different role in mitosis: both act phenotypically as spatial regulators to ensure that mitotic spindle, nuclear membrane, and nuclear pore assembly occur exclusively around chromatin. Importin β is known to act by repressing assembly factors in regions distant from chromatin, whereas RanGTP produced on chromatin frees factors from importin β for localized assembly. The mechanism of transportin regulation was unknown. Diametrically opposed models for transportin action are as follows: 1) indirect action by RanGTP sequestration, thus down-regulating release of assembly factors from importin β, and 2) direct action by transportin binding and inhibiting assembly factors. Experiments in Xenopus assembly extracts with M9M, a superaffinity nuclear localization sequence that displaces cargoes bound by transportin, or TLB, a mutant transportin that can bind cargo and RanGTP simultaneously, support direct inhibition. Consistently, simple addition of M9M to mitotic cytosol induces microtubule aster assembly. ELYS and the nucleoporin 107–160 complex, components of mitotic kinetochores and nuclear pores, are blocked from binding to kinetochores in vitro by transportin, a block reversible by M9M. In vivo, 30% of M9M-transfected cells have spindle/cytokinesis defects. We conclude that the cell contains importin β and transportin “global positioning system”or “GPS” pathways that are mechanistically parallel. PMID:24478460

  14. The Arabidopsis LYST INTERACTING PROTEIN 5 Acts in Regulating Abscisic Acid Signaling and Drought Response.

    PubMed

    Xia, Zongliang; Huo, Yongjin; Wei, Yangyang; Chen, Qiansi; Xu, Ziwei; Zhang, Wei

    2016-01-01

    Multivesicular bodies (MVBs) are unique endosomes containing vesicles in the lumens and play essential roles in many eukaryotic cellular processes. The Arabidopsis LYST INTERACTING PROTEIN 5 (LIP5), a positive regulator of MVB biogenesis, has critical roles in biotic and abiotic stress responses. However, whether the abscisic acid (ABA) signaling is involved in LIP5-mediated stress response is largely unknown. Here, we report that LIP5 functions in regulating ABA signaling and drought response in Arabidopsis. Analyses of a LIP5 promoter-β-glucuronidase (GUS) construct revealed substantial GUS activity in whole seedlings. The expression of LIP5 was induced by ABA and drought, and overexpression of LIP5 led to ABA hypersensitivity, enhanced stomatal closure, reduced water loss, and, therefore, increased drought tolerance. On the contrary, LIP5 knockdown mutants showed ABA-insensitive phenotypes and reduced drought tolerance; suggesting that LIP5 acts in regulating ABA response. Further analysis using a fluorescent dye revealed that ABA and water stress induced cell endocytosis or vesicle trafficking in a largely LIP5-dependent manner. Furthermore, expression of several drought- or ABA-inducible marker genes was significantly down-regulated in the lip5 mutant seedlings. Collectively, our data suggest that LIP5 positively regulates drought tolerance through ABA-mediated cell signaling. PMID:27313589

  15. The Arabidopsis LYST INTERACTING PROTEIN 5 Acts in Regulating Abscisic Acid Signaling and Drought Response

    PubMed Central

    Xia, Zongliang; Huo, Yongjin; Wei, Yangyang; Chen, Qiansi; Xu, Ziwei; Zhang, Wei

    2016-01-01

    Multivesicular bodies (MVBs) are unique endosomes containing vesicles in the lumens and play essential roles in many eukaryotic cellular processes. The Arabidopsis LYST INTERACTING PROTEIN 5 (LIP5), a positive regulator of MVB biogenesis, has critical roles in biotic and abiotic stress responses. However, whether the abscisic acid (ABA) signaling is involved in LIP5-mediated stress response is largely unknown. Here, we report that LIP5 functions in regulating ABA signaling and drought response in Arabidopsis. Analyses of a LIP5 promoter-β-glucuronidase (GUS) construct revealed substantial GUS activity in whole seedlings. The expression of LIP5 was induced by ABA and drought, and overexpression of LIP5 led to ABA hypersensitivity, enhanced stomatal closure, reduced water loss, and, therefore, increased drought tolerance. On the contrary, LIP5 knockdown mutants showed ABA-insensitive phenotypes and reduced drought tolerance; suggesting that LIP5 acts in regulating ABA response. Further analysis using a fluorescent dye revealed that ABA and water stress induced cell endocytosis or vesicle trafficking in a largely LIP5-dependent manner. Furthermore, expression of several drought- or ABA-inducible marker genes was significantly down-regulated in the lip5 mutant seedlings. Collectively, our data suggest that LIP5 positively regulates drought tolerance through ABA-mediated cell signaling. PMID:27313589

  16. 77 FR 30998 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Government...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-24

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Government Property AGENCY: Defense Acquisition Regulations System, Department of... and Technical Information System,'' and is used when directed by the plant clearance officer. e....

  17. 77 FR 43076 - Federal Acquisition Regulation; Information Collection; Value Engineering Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-23

    ... Regulation; Information Collection; Value Engineering Requirements AGENCIES: Department of Defense (DOD... approved information collection requirement concerning Value Engineering Requirements. Public comments are...: Submit comments identified by Information Collection 9000- 0027, Value Engineering Requirements, by...

  18. Identification of trans-acting factors regulating SamDC expression in Oryza sativa

    SciTech Connect

    Basu, Supratim; Roychoudhury, Aryadeep; Sengupta, Dibyendu N.

    2014-03-07

    Highlights: • Identification of cis elements responsible for SamDC expression by in silico analysis. • qPCR analysis of SamDC expression to abiotic and biotic stress treatments. • Detection of SamDC regulators using identified cis-elements as probe by EMSA. • Southwestern Blot analysis to predict the size of the trans-acting factors. - Abstract: Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silico analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the trans-acting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression.

  19. 75 FR 45104 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Contract...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-02

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition... Regulations System, Department of Defense (DoD). ACTION: Notice and request for comments. SUMMARY: In... announces the proposed extension of a public information collection requirement and seeks public comment...

  20. 75 FR 68331 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DFARS Part...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-05

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition... Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and request for comments regarding a proposed extension of an approved information collection requirement. SUMMARY: In compliance...

  1. 75 FR 26739 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 244...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition...: Defense Acquisition Regulations System, Department of Defense. ACTION: Notice and request for comments regarding a proposed extension of an approved information collection requirement. SUMMARY: In...

  2. 78 FR 17186 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-20

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition... Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and request for comments regarding a proposed extension of an approved information collection requirement. SUMMARY: In compliance...

  3. 75 FR 10790 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-09

    ... Regulation Supplement; Notification Requirements for Critical Safety Items AGENCY: Defense Acquisition... Supplement (DFARS) Notification Requirements for Critical Safety Items; OMB Control Number 0704-0441....

  4. The European "clinical trial" regulation; relationship with the Jardé Act: a Giens workshop.

    PubMed

    Lemaire, François; Marchenay, Brigitte; Chassany, Olivier; Barthélémy, Philippe; Bouzzagou, Mohamed; Comet, Denis; Delval, Cécile; Dubray, Claude; Fouret, Cécile; Frija-Orvoen, Elisabeth; Gambotti, Laetitia; Lamarque, Véronique; d'Orsay, Geneviève; Plattner, Valérie; Sibenaler, Claire; Roux, Jacques; Thoby, Frédérique

    2015-01-01

    In May 2014, the European Union Parliament and Council published a new regulation on clinical trials on medicinal products for human use, which is designed to replace Directive 2001/20/EC. It will not come into effect until 2016. Nevertheless, it is essential to examine its relationship with national legislation, i.e. the Jardé Act, whose implementation has been delayed pending publication of the European regulation. The Giens workshop identified and examined the various issues that this relationship is bound to raise. In particular, it looked at trial methodology assessment procedures, the working relationship between the French National Agency of Drug Safety and Health Products (Agence Nationale de Sécurité du Médicament et des Produits de Santé, ANSM) and ethics committees during the authorization application evaluation phase, review of post-authorization/registration studies on medicinal products and medical devices, and data transparency. PMID:25679191

  5. Thyroid hormone is required for hypothalamic neurons regulating cardiovascular functions

    PubMed Central

    Mittag, Jens; Lyons, David J.; Sällström, Johan; Vujovic, Milica; Dudazy-Gralla, Susi; Warner, Amy; Wallis, Karin; Alkemade, Anneke; Nordström, Kristina; Monyer, Hannah; Broberger, Christian; Arner, Anders; Vennström, Björn

    2012-01-01

    Thyroid hormone is well known for its profound direct effects on cardiovascular function and metabolism. Recent evidence, however, suggests that the hormone also regulates these systems indirectly through the central nervous system. While some of the molecular mechanisms underlying the hormone’s central control of metabolism have been identified, its actions in the central cardiovascular control have remained enigmatic. Here, we describe a previously unknown population of parvalbuminergic neurons in the anterior hypothalamus that requires thyroid hormone receptor signaling for proper development. Specific stereotaxic ablation of these cells in the mouse resulted in hypertension and temperature-dependent tachycardia, indicating a role in the central autonomic control of blood pressure and heart rate. Moreover, the neurons exhibited intrinsic temperature sensitivity in patch-clamping experiments, providing a new connection between cardiovascular function and core temperature. Thus, the data identify what we believe to be a novel hypothalamic cell population potentially important for understanding hypertension and indicate developmental hypothyroidism as an epigenetic risk factor for cardiovascular disorders. Furthermore, the findings may be beneficial for treatment of the recently identified patients that have a mutation in thyroid hormone receptor α1. PMID:23257356

  6. translin Is Required for Metabolic Regulation of Sleep.

    PubMed

    Murakami, Kazuma; Yurgel, Maria E; Stahl, Bethany A; Masek, Pavel; Mehta, Aradhana; Heidker, Rebecca; Bollinger, Wesley; Gingras, Robert M; Kim, Young-Joon; Ja, William W; Suter, Beat; DiAngelo, Justin R; Keene, Alex C

    2016-04-01

    Dysregulation of sleep or feeding has enormous health consequences. In humans, acute sleep loss is associated with increased appetite and insulin insensitivity, while chronically sleep-deprived individuals are more likely to develop obesity, metabolic syndrome, type II diabetes, and cardiovascular disease. Conversely, metabolic state potently modulates sleep and circadian behavior; yet, the molecular basis for sleep-metabolism interactions remains poorly understood. Here, we describe the identification of translin (trsn), a highly conserved RNA/DNA binding protein, as essential for starvation-induced sleep suppression. Strikingly, trsn does not appear to regulate energy stores, free glucose levels, or feeding behavior suggesting the sleep phenotype of trsn mutant flies is not a consequence of general metabolic dysfunction or blunted response to starvation. While broadly expressed in all neurons, trsn is transcriptionally upregulated in the heads of flies in response to starvation. Spatially restricted rescue or targeted knockdown localizes trsn function to neurons that produce the tachykinin family neuropeptide Leucokinin. Manipulation of neural activity in Leucokinin neurons revealed these neurons to be required for starvation-induced sleep suppression. Taken together, these findings establish trsn as an essential integrator of sleep and metabolic state, with implications for understanding the neural mechanism underlying sleep disruption in response to environmental perturbation. PMID:27020744

  7. Knowledge of the animal welfare act and animal welfare regulations influences attitudes toward animal research.

    PubMed

    Metzger, Mitchell M

    2015-01-01

    Recent public-opinion polls indicate that Americans have shown a decline in support for animal experimentation, and several reports suggest a relationship between people's knowledge of animal welfare regulations and their attitudes toward animal research. Therefore, this study was designed to assess respondent's knowledge of several provisions in the Animal Welfare Act (AWA) and Animal Welfare Regulations (AWR), and determine whether exposure to elements of this legislation would influence an individual's attitudes toward the use of animals in research. A survey was used to assess knowledge of animal research regulations and attitudes toward animal research from a sample of individuals recruited through Amazon's Mechanical Turk crowdsourcing marketplace. Results from study 1 confirmed the hypothesis that respondents had little knowledge of various federal regulations that govern animal research activities. Data from study 2 revealed that exposure to elements of the AWA and AWR influenced participants' attitudes toward the use of animals in research. These results suggest that providing information to the general public about the AWA and AWR that protect laboratory animals from abuse and neglect may help alleviate concerns about using animals in research settings. PMID:25651094

  8. Knowledge of the Animal Welfare Act and Animal Welfare Regulations Influences Attitudes toward Animal Research

    PubMed Central

    2015-01-01

    Recent public-opinion polls indicate that Americans have shown a decline in support for animal experimentation, and several reports suggest a relationship between people's knowledge of animal welfare regulations and their attitudes toward animal research. Therefore, this study was designed to assess respondent's knowledge of several provisions in the Animal Welfare Act (AWA) and Animal Welfare Regulations (AWR), and determine whether exposure to elements of this legislation would influence an individual's attitudes toward the use of animals in research. A survey was used to assess knowledge of animal research regulations and attitudes toward animal research from a sample of individuals recruited through Amazon's Mechanical Turk crowdsourcing marketplace. Results from study 1 confirmed the hypothesis that respondents had little knowledge of various federal regulations that govern animal research activities. Data from study 2 revealed that exposure to elements of the AWA and AWR influenced participants’ attitudes toward the use of animals in research. These results suggest that providing information to the general public about the AWA and AWR that protect laboratory animals from abuse and neglect may help alleviate concerns about using animals in research settings. PMID:25651094

  9. APP intracellular domain acts as a transcriptional regulator of miR-663 suppressing neuronal differentiation

    PubMed Central

    Shu, R; Wong, W; Ma, Q H; Yang, Z Z; Zhu, H; Liu, F J; Wang, P; Ma, J; Yan, S; Polo, J M; Bernard, C C A; Stanton, L W; Dawe, G S; Xiao, Z C

    2015-01-01

    Amyloid precursor protein (APP) is best known for its involvement in the pathogenesis of Alzheimer's disease. We have previously demonstrated that APP intracellular domain (AICD) regulates neurogenesis; however, the mechanisms underlying AICD-mediated regulation of neuronal differentiation are not yet fully characterized. Using genome-wide chromatin immunoprecipitation approaches, we found that AICD is specifically recruited to the regulatory regions of several microRNA genes, and acts as a transcriptional regulator for miR-663, miR-3648 and miR-3687 in human neural stem cells. Functional assays show that AICD negatively modulates neuronal differentiation through miR-663, a primate-specific microRNA. Microarray data further demonstrate that miR-663 suppresses the expression of multiple genes implicated in neurogenesis, including FBXL18 and CDK6. Our results indicate that AICD has a novel role in suppression of neuronal differentiation via transcriptional regulation of miR-663 in human neural stem cells. PMID:25695604

  10. Identification of trans-acting factors regulating SamDC expression in Oryza sativa.

    PubMed

    Basu, Supratim; Roychoudhury, Aryadeep; Sengupta, Dibyendu N

    2014-03-01

    Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silico analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the trans-acting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression. PMID:24530223

  11. 76 FR 39007 - Native American Graves Protection and Repatriation Act Regulations- Definition of “Indian Tribe”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-05

    ... Office of the Secretary of the Interior 43 CFR Part 10 RIN 1024-AD98 Native American Graves Protection... regulations implementing the Native American Graves Protection and Repatriation Act (NAGPRA) removes the... implementation of the Native American Graves Protection and Repatriation Act, including the issuance...

  12. DOCK2 and DOCK5 Act Additively in Neutrophils To Regulate Chemotaxis, Superoxide Production, and Extracellular Trap Formation

    PubMed Central

    Watanabe, Mayuki; Terasawa, Masao; Miyano, Kei; Yanagihara, Toyoshi; Uruno, Takehito; Sanematsu, Fumiyuki; Nishikimi, Akihiko; Côté, Jean-François; Sumimoto, Hideki; Fukui, Yoshinori

    2015-01-01

    Neutrophils are highly motile leukocytes that play important roles in the innate immune response to invading pathogens. Neutrophils rapidly migrate to the site of infections and kill pathogens by producing reactive oxygen species (ROS). Neutrophil chemotaxis and ROS production require activation of Rac small GTPase. DOCK2, an atypical guanine nucleotide exchange factor (GEF), is one of the major regulators of Rac in neutrophils. However, because DOCK2 deficiency does not completely abolish fMLF-induced Rac activation, other Rac GEFs may also participate in this process. In this study, we show that DOCK5 acts with DOCK2 in neutrophils to regulate multiple cellular functions. We found that fMLF- and PMA-induced Rac activation were almost completely lost in mouse neutrophils lacking both DOCK2 and DOCK5. Although β2 integrin–mediated adhesion occurred normally even in the absence of DOCK2 and DOCK5, mouse neutrophils lacking DOCK2 and DOCK5 exhibited a severe defect in chemotaxis and ROS production. Similar results were obtained when human neutrophils were treated with CPYPP, a small-molecule inhibitor of these DOCK GEFs. Additionally, we found that DOCK2 and DOCK5 regulate formation of neutrophil extracellular traps (NETs). Because NETs are involved in vascular inflammation and autoimmune responses, DOCK2 and DOCK5 would be a therapeutic target for controlling NET-mediated inflammatory disorders. PMID:25339677

  13. The conserved Misshapen-Warts-Yorkie pathway acts in enteroblasts to regulate intestinal stem cells in Drosophila

    PubMed Central

    Li, Qi; Li, Shuangxi; Mana-Capelli, Sebastian; Roth Flach, Rachel J.; Danai, Laura V.; Amcheslavsky, Alla; Nie, Yingchao; Kaneko, Satoshi; Yao, Xiaohao; Chen, Xiaochu; Cotton, Jennifer L.; Mao, Junhao; McCollum, Dannel; Jiang, Jin; Czech, Michael P.; Xu, Lan; Ip, Y. Tony

    2014-01-01

    SUMMARY Similar to the mammalian intestine, the Drosophila adult midgut has resident stem cells that support growth and regeneration. How the niche regulates intestinal stem cell activity in both mammals and flies is not well understood. Here we show that the conserved germinal center protein kinase Misshapen restricts intestinal stem cell division by repressing the expression of the JAK-STAT pathway ligand Upd3 in differentiating enteroblasts. Misshapen, a distant relative to the prototypic Warts activating kinase Hippo, interacts with and activates Warts to negatively regulate the activity of Yorkie and the expression of Upd3. The mammalian Misshapen homolog MAP4K4 similarly interacts with LATS (Warts homolog) and promotes inhibition of YAP (Yorkie homolog). Together, this work reveals that the Misshapen-Warts-Yorkie pathway acts in enteroblasts to control niche signaling to intestinal stem cells. These findings also provide a model in which to study requirements for MAP4K4-related kinases in MST1/2-independent regulation of LATS and YAP. PMID:25453828

  14. The Global Regulator CodY Regulates Toxin Gene Expression in Bacillus anthracis and Is Required for Full Virulence▿ †

    PubMed Central

    van Schaik, Willem; Château, Alice; Dillies, Marie-Agnès; Coppée, Jean-Yves; Sonenshein, Abraham L.; Fouet, Agnès

    2009-01-01

    In gram-positive bacteria, CodY is an important regulator of genes whose expression changes upon nutrient limitation and acts as a repressor of virulence gene expression in some pathogenic species. Here, we report the role of CodY in Bacillus anthracis, the etiologic agent of anthrax. Disruption of codY completely abolished virulence in a toxinogenic, noncapsulated strain, indicating that the activity of CodY is required for full virulence of B. anthracis. Global transcriptome analysis of a codY mutant and the parental strain revealed extensive differences. These differences could reflect direct control for some genes, as suggested by the presence of CodY binding sequences in their promoter regions, or indirect effects via the CodY-dependent control of other regulatory proteins or metabolic rearrangements in the codY mutant strain. The differences included reduced expression of the anthrax toxin genes in the mutant strain, which was confirmed by lacZ reporter fusions and immunoblotting. The accumulation of the global virulence regulator AtxA protein was strongly reduced in the mutant strain. However, in agreement with the microarray data, expression of atxA, as measured using an atxA-lacZ transcriptional fusion and by assaying atxA mRNA, was not significantly affected in the codY mutant. An atxA-lacZ translational fusion was also unaffected. Overexpression of atxA restored toxin component synthesis in the codY mutant strain. These results suggest that CodY controls toxin gene expression by regulating AtxA accumulation posttranslationally. PMID:19651859

  15. The type I activin receptor ActRIB is required for egg cylinder organization and gastrulation in the mouse

    PubMed Central

    Gu, Zhenyu; Nomura, Masatoshi; Simpson, Brenda B.; Lei, Hong; Feijen, Alie; van den Eijnden-van Raaij, Janny; Donahoe, Patricia K.; Li, En

    1998-01-01

    ActRIB is a type I transmembrane serine/threonine kinase receptor that has been shown to form heteromeric complexes with the type II activin receptors to mediate activin signal. To investigate the function of ActRIB in mammalian development, we generated ActRIB-deficient ES cell lines and mice by gene targeting. Analysis of the ActRIB−/− embryos showed that the epiblast and the extraembryonic ectoderm were disorganized, resulting in disruption and developmental arrest of the egg cylinder before gastrulation. To assess the function of ActRIB in mesoderm formation and gastrulation, chimera analysis was conducted. We found that ActRIB−/− ES cells injected into wild-type blastocysts were able to contribute to the mesoderm in chimeric embryos, suggesting that ActRIB is not required for mesoderm formation. Primitive streak formation, however, was impaired in chimeras when ActRIB−/− cells contributed highly to the epiblast. Further, chimeras generated by injection of wild-type ES cells into ActRIB−/− blastocysts formed relatively normal extraembryonic tissues, but the embryo proper developed poorly probably resulting from severe gastrulation defect. These results provide genetic evidence that ActRIB functions in both epiblast and extraembryonic cells to mediate signals that are required for egg cylinder organization and gastrulation. PMID:9512518

  16. 47 CFR 80.159 - Operator requirements of Title III of the Communications Act and the Safety Convention.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Operator requirements of Title III of the Communications Act and the Safety Convention. 80.159 Section 80.159 Telecommunication FEDERAL COMMUNICATIONS... Communications Act and the Safety Convention. (a) Each telegraphy passenger ship equipped with a...

  17. 15 CFR Supplement No. 1 to Part 730 - Information Collection Requirements Under the Paperwork Reduction Act: OMB Control Numbers

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Under the Paperwork Reduction Act: OMB Control Numbers No. Supplement No. 1 to Part 730 Commerce and... Supplement No. 1 to Part 730—Information Collection Requirements Under the Paperwork Reduction Act: OMB...-0033 License Exception, Humanitarian Donations §§ 740.12(b)(7), 762.2(b), Supp. No. 2 to part 740....

  18. Implementation of the Comprehensive Methamphetamine Control Act of 1996; regulation of pseudoephedrine, phenylpropanolamine, and combination ephedrine drug products and reports of certain transactions to nonregulated persons. Final rule.

    PubMed

    2002-03-28

    DEA is amending its regulations to implement the requirements of the Comprehensive Methamphetamine Control Act of 1996 (MCA) with respect to the regulation of pseudoephedrine, phenylpropanolamine, and combination ephedrine drug products as List I chemicals, and the reporting of certain transactions involving pseudoephedrine, phenylpropanolamine, and combination ephedrine drug products. The MCA removed the previous exemption from regulation as List I chemicals which had applied to pseudoephedrine, phenylpropanolamine, and combination ephedrine drug products. This action makes persons who distribute the products subject to the registration requirement. Also, distributions, importations, and exportations of the products became subject to the existing chemical controls relating to regulated transactions, except in certain circumstances specified in the MCA. The MCA also requires that reports be submitted for certain distributions involving pseudoephedrine, phenylpropanolamine, and ephedrine (including drug products containing those chemicals) by Postal Service or private or commercial carrier to nonregulated persons. This final rule amends the regulations to make them consistent with the language of the MCA and to establish specific procedures to be followed to satisfy the new reporting requirement. DEA has, where possible, taken action to limit the public impact of these new requirements while remaining consistent with the intent of the MCA to attack the diversion of regulated drug products to the clandestine manufacture of methamphetamine. PMID:11922057

  19. Discovery of a Splicing Regulator Required for Cell Cycle Progression

    SciTech Connect

    Suvorova, Elena S.; Croken, Matthew; Kratzer, Stella; Ting, Li-Min; Conde de Felipe, Magnolia; Balu, Bharath; Markillie, Lye Meng; Weiss, Louis M.; Kim, Kami; White, Michael W.

    2013-02-01

    In the G1 phase of the cell division cycle, eukaryotic cells prepare many of the resources necessary for a new round of growth including renewal of the transcriptional and protein synthetic capacities and building the machinery for chromosome replication. The function of G1 has an early evolutionary origin and is preserved in single and multicellular organisms, although the regulatory mechanisms conducting G1 specific functions are only understood in a few model eukaryotes. Here we describe a new G1 mutant from an ancient family of apicomplexan protozoans. Toxoplasma gondii temperature-sensitive mutant 12-109C6 conditionally arrests in the G1 phase due to a single point mutation in a novel protein containing a single RNA-recognition-motif (TgRRM1). The resulting tyrosine to asparagine amino acid change in TgRRM1 causes severe temperature instability that generates an effective null phenotype for this protein when the mutant is shifted to the restrictive temperature. Orthologs of TgRRM1 are widely conserved in diverse eukaryote lineages, and the human counterpart (RBM42) can functionally replace the missing Toxoplasma factor. Transcriptome studies demonstrate that gene expression is downregulated in the mutant at the restrictive temperature due to a severe defect in splicing that affects both cell cycle and constitutively expressed mRNAs. The interaction of TgRRM1 with factors of the tri-SNP complex (U4/U6 & U5 snRNPs) indicate this factor may be required to assemble an active spliceosome. Thus, the TgRRM1 family of proteins is an unrecognized and evolutionarily conserved class of splicing regulators. This study demonstrates investigations into diverse unicellular eukaryotes, like the Apicomplexa, have the potential to yield new insights into important mechanisms conserved across modern eukaryotic kingdoms.

  20. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Are my HCT/P's regulated solely under section 361... Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS,...

  1. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Are my HCT/P's regulated solely under section 361... Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS,...

  2. Mixed Waste Integrated Program: Demonstrating technologies to meet the requirements of the Federal Facility Compliance Act

    SciTech Connect

    Berry, J.B.

    1994-07-01

    Mixed waste is defined as ``waste contaminated with chemically hazardous [governed by the Resource Conservation and Recovery Act (RCRA)] and radioactive species [governed by US Department of energy (DOE) orders].`` The Mixed Waste Integrated Program (MWIP) is responding to the need for DOE mixed-waste treatment technologies tat meet these dual regulatory requirements. MWIP is developing emerging and innovative treatment technologies to determine process feasibility. Technology demonstrations of fixed-hearth plasma arc and vitrification systems will be used to determine whether these processes are superior to existing technologies in reducing risk, minimizing life-cycle cost, and improving process performance. MWIP also provides a forum for stakeholder and customer involvement in the technology development process.

  3. Eya 1 acts as a critical regulator for specifying the metanephric mesenchyme.

    PubMed

    Sajithlal, Gangadharan; Zou, Dan; Silvius, Derek; Xu, Pin-Xian

    2005-08-15

    Although it is well established that the Gdnf-Ret signal transduction pathway initiates metanephric induction, no single regulator has yet been identified to specify the metanephric mesenchyme or blastema within the intermediate mesoderm, the earliest step of metanephric kidney development and the molecular mechanisms controlling Gdnf expression are essentially unknown. Previous studies have shown that a loss of Eya 1 function leads to renal agenesis that is a likely result of failure of metanephric induction. The studies presented here demonstrate that Eya 1 specifies the metanephric blastema within the intermediate mesoderm at the caudal end of the nephrogenic cord. In contrast to its specific roles in metanephric development, Eya 1 appears dispensable for the formation of nephric duct and mesonephric tubules. Using a combination of null and hypomorphic Eya 1 mutants, we now demonstrated that approximately 20% of normal Eya 1 protein level is sufficient for establishing the metanephric blastema and inducing the ureteric bud formation but not for its normal branching. Using Eya 1, Gdnf, Six 1 and Pax 2 mutant mice, we show that Eya 1 probably functions at the top of the genetic hierarchy controlling kidney organogenesis and it acts in combination with Six 1 and Pax 2 to regulate Gdnf expression during UB outgrowth and branching. These findings uncover an essential function for Eya 1 as a critical determination factor in acquiring metanephric fate within the intermediate mesoderm and as a key regulator of Gdnf expression during ureteric induction and branching morphogenesis. PMID:16018995

  4. Epithelial IL-1R2 acts as a homeostatic regulator during remission of ulcerative colitis

    PubMed Central

    Mora-Buch, R; Dotti, I; Planell, N; Calderón-Gómez, E; Jung, P; Masamunt, M C; Llach, J; Ricart, E; Batlle, E; Panés, J; Salas, A

    2016-01-01

    Ulcerative colitis (UC) is a chronic intestinal inflammatory disease that may undergo periods of activity followed by remission. We aimed to identify the endogenous regulatory mechanisms that may promote disease remission. Transcriptional and protein analysis of the intestinal mucosa revealed that the IL-1 decoy receptor, interleukin-1 receptor type 2 (IL1R2), was upregulated in remission compared with active UC and controls. We identified epithelial cells as being responsible for increased IL-1R2 production during remission. Expression of IL1R2 was negatively regulated by Wnt/beta-catenin signals in colonic crypts or epithelial stem cell cultures; accordingly, epithelial stem cells upregulated IL-1R2 upon differentiation. Blocking IL-1R2 in isolated colonic crypt cultures of UC patients in remission and T-cell cultures stimulated with biopsy supernatant from UC patients in remission boosted IL-1β-dependent production of inflammation-related cytokines. Finally, IL1R2 transcription was significantly lower in patients that relapsed during a 1-year follow-up period compared with those in endoscopic remission. Collectively, our results reveal that the IL-1/IL-1R2 axis is differentially regulated in the remitting intestinal mucosa of UC patients. We hypothesize that IL-1R2 in the presence of low concentrations of IL-1β may act locally as a regulator of intestinal homeostasis. PMID:26530134

  5. 77 FR 67366 - Federal Acquisition Regulation; Information Collection; Quality Assurance Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-09

    ... Regulation; Information Collection; Quality Assurance Requirements AGENCY: Department of Defense (DOD... information collection requirement concerning quality assurance requirements. Public comments are particularly... and methodology; ways to enhance the quality, utility, and clarity of the information to be...

  6. Proper gibberellin localization in vascular tissue is required to regulate adventitious root development in tobacco.

    PubMed

    Niu, Shihui; Li, Zhexin; Yuan, Huwei; Fang, Pan; Chen, Xiaoyang; Li, Wei

    2013-08-01

    Bioactive gibberellins (GAs) are involved in many developmental aspects of the life cycle of plants, acting either directly or through interaction with other hormones. Accumulating evidence suggests that GAs have an important effect on root growth; however, there is currently little information on the specific regulatory mechanism of GAs during adventitious root development. A study was conducted on tobacco (Nicotiana tabacum) plants for altered rates of biosynthesis, catabolism, and GA signalling constitutively or in specific tissues using a transgenic approach. In the present study, PtGA20ox, PtGA2ox1, and PtGAI were overexpressed under the control of the 35S promoter, vascular cambium-specific promoter (LMX5), or root meristem-specific promoter (TobRB7), respectively. Evidence is provided that the precise localization of bioactive GA in the stem but not in the roots is required to regulate adventitious root development in tobacco. High levels of GA negatively regulate the early initiation step of root formation through interactions with auxin, while a proper and mobile GA signal is required for the emergence and subsequent long-term elongation of established primordia. The results demonstrated that GAs have an inhibitory effect on adventitious root formation but a stimulatory effect on root elongation. PMID:23918971

  7. 78 FR 30296 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The Commission's regulations include a methodology for oil...

  8. 77 FR 29997 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The Commission's regulations include a methodology for oil...

  9. 76 FR 28966 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The Commission's regulations include a methodology for oil...

  10. Compliance with the Clean Air Act Title VI Stratospheric Ozone Protection Program requirements at U.S. DOE Oak Ridge Reservation Facilities

    SciTech Connect

    Humphreys, M.P.; Atkins, E.M.

    1999-07-01

    The Title VI Stratospheric Ozone Protection Program of the Clean Air Act (CAA) requires promulgation of regulations to reduce and prevent damage to the earth's protective ozone layer. Regulations pursuant to Title VI of the CAA are promulgated in the Code of Federal Regulations (CFR) at Title 40 CFR, Part 822. The regulations include ambitious production phaseout schedules for ozone depleting substances (ODS) including chlorofluorocarbons (CFCs), hydrochlorofluorocarbons (HCFCs), halons, carbon tetrachloride, and methyl chloroform under 40 CFR 82, Subpart A. The regulations also include requirements for recycling and emissions reduction during the servicing of refrigeration equipment and technician certification requirements under Subpart F; provisions for servicing of motor vehicle air conditioners under Subpart B; a ban on nonessential products containing Class 1 ODS under Subpart C; restrictions on Federal procurement of ODS under Subpart D; labeling of products using ODS under Subpart E; and the Significant New Alternatives Policy Program under Subpart G. This paper will provide details of initiatives undertaken at US Department of Energy (DOE) Oak Ridge Reservation (ORR) Facilities for implementation of requirements under the Title VI Stratospheric Ozone Protection Program. The Stratospheric Ozone Protection Plans include internal DOE requirements for: (1) maintenance of ODS inventories; (2) ODS procurement practices; (3) servicing of refrigeration and air conditioning equipment; (4) required equipment modifications or replacement; (5) technician certification training; (6) labeling of products containing ODS; (7) substitution of chlorinated solvents; and (8) replacement of halon fire protection systems. The plans also require establishment of administrative control systems which assure that compliance is achieved and maintained as the regulations continue to develop and become effective.

  11. Diversification of a Transcription Factor Family Led to the Evolution of Antagonistically Acting Genetic Regulators of Root Hair Growth.

    PubMed

    Breuninger, Holger; Thamm, Anna; Streubel, Susanna; Sakayama, Hidetoshi; Nishiyama, Tomoaki; Dolan, Liam

    2016-06-20

    Streptophytes colonized the land some time before 470 million years ago [1-3]. The colonization coincided with an increase in morphological and cellular diversity [4-7]. This increase in diversity is correlated with a proliferation in transcription factors encoded in genomes [8-10]. This suggests that gene duplication and subsequent diversification of function was instrumental in the generation of land plant diversity. Here, we investigate the diversification of the streptophyte-specific Lotus japonicus ROOTHAIRLESS LIKE (LRL) transcription factor (TF) [11, 12] subfamily of basic loop helix (bHLH) proteins by comparing gene function in early divergent and derived land plant species. We report that the single Marchantia polymorpha LRL gene acts as a general growth regulator required for rhizoid development, a function that has been partially conserved throughout multicellular streptophytes. In contrast, the five relatively derived Arabidopsis thaliana LRL genes comprise two antagonistically acting groups of differentially expressed genes. The diversification of LRL genes accompanied the evolution of an antagonistic regulatory element controlling root hair development. PMID:27265398

  12. 78 FR 19467 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-01

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS); DoD Pilot Mentor-Prot g Program AGENCY: Defense Acquisition Regulations System... approved information collection requirement. SUMMARY: In compliance with Section 3506(c)(2)(A) of...

  13. 78 FR 60898 - Regulation on Definition and Requirements for a Nationally Recognized Testing Laboratory...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    ...OSHA requests comments concerning its proposed revision and extension of the information collection requirements specified by its Regulation at 29 CFR 1910.7, ``definition and requirements for a nationally recognized testing laboratory'' (The Regulation). The Regulation specifies procedures that organizations must follow to apply for, and to maintain, OSHA's recognition to test and certify......

  14. 16 CFR 301.2 - General requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.2 General requirements. (a) Each and every fur... with the requirements of the act and rules and regulations. (b) Each and every fur shall be invoiced...

  15. 16 CFR 301.2 - General requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.2 General requirements. (a) Each and every fur... with the requirements of the act and rules and regulations. (b) Each and every fur shall be invoiced...

  16. 16 CFR 301.2 - General requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.2 General requirements. (a) Each and every fur... with the requirements of the act and rules and regulations. (b) Each and every fur shall be invoiced...

  17. Enhancer of zeste acts as a major developmental regulator of Ciona intestinalis embryogenesis

    PubMed Central

    Le Goff, Emilie; Martinand-Mari, Camille; Martin, Marianne; Feuillard, Jérôme; Boublik, Yvan; Godefroy, Nelly; Mangeat, Paul; Baghdiguian, Stephen; Cavalli, Giacomo

    2015-01-01

    ABSTRACT The paradigm of developmental regulation by Polycomb group (PcG) proteins posits that they maintain silencing outside the spatial expression domains of their target genes, particularly of Hox genes, starting from mid embryogenesis. The Enhancer of zeste [E(z)] PcG protein is the catalytic subunit of the PRC2 complex, which silences its targets via deposition of the H3K27me3 mark. Here, we studied the ascidian Ciona intestinalis counterpart of E(z). Ci-E(z) is detected by immunohistochemistry as soon as the 2- and 4-cell stages as a cytoplasmic form and becomes exclusively nuclear thereafter, whereas the H3K27me3 mark is detected starting from the gastrula stage and later. Morpholino invalidation of Ci-E(z) leads to the total disappearance of both Ci-E(z) protein and its H3K27me3 mark. Ci-E(z) morphants display a severe phenotype. Strikingly, the earliest defects occur at the 4-cell stage with the dysregulation of cell positioning and mitotic impairment. At later stages, Ci-E(z)-deficient embryos are affected by terminal differentiation defects of neural, epidermal and muscle tissues, by the failure to form a notochord and by the absence of caudal nerve. These major phenotypic defects are specifically rescued by injection of a morpholino-resistant Ci-E(z) mRNA, which restores expression of Ci-E(z) protein and re-deposition of the H3K27me3 mark. As observed by qPCR analyses, Ci-E(z) invalidation leads to the early derepression of tissue-specific developmental genes, whereas late-acting developmental genes are generally down-regulated. Altogether, our results suggest that Ci-E(z) plays a major role during embryonic development in Ciona intestinalis by silencing early-acting developmental genes in a Hox-independent manner. PMID:26276097

  18. Aurora-A acts as a tumor suppressor and regulates self-renewal of Drosophila neuroblasts

    PubMed Central

    Wang, Hongyan; Somers, Gregory W.; Bashirullah, Arash; Heberlein, Ulrike; Yu, Fengwei; Chia, William

    2006-01-01

    The choice of self-renewal versus differentiation is a fundamental issue in stem cell and cancer biology. Neural progenitors of the Drosophila post-embryonic brain, larval neuroblasts (NBs), divide asymmetrically in a stem cell-like fashion to generate a self-renewing NB and a Ganglion Mother Cell (GMC), which divides terminally to produce two differentiating neuronal/glial daughters. Here we show that Aurora-A (AurA) acts as a tumor suppressor by suppressing NB self-renewal and promoting neuronal differentiation. In aurA loss-of-function mutants, supernumerary NBs are produced at the expense of neurons. AurA suppresses tumor formation by asymmetrically localizing atypical protein kinase C (aPKC), an NB proliferation factor. Numb, which also acts as a tumor suppressor in larval brains, is a major downstream target of AurA and aPKC. Notch activity is up-regulated in aurA and numb larval brains, and Notch signaling is necessary and sufficient to promote NB self-renewal and suppress differentiation in larval brains. Our data suggest that AurA, aPKC, Numb, and Notch function in a pathway that involved a series of negative genetic interactions. We have identified a novel mechanism for controlling the balance between self-renewal and neuronal differentiation during the asymmetric division of Drosophila larval NBs. PMID:17182870

  19. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... and envelope or any fax cover sheet should be clearly marked “Privacy Act Request.” A Privacy Act... mail or by fax, or delivered in person, a Privacy Act request will not be considered to have been... or name, author, recipient, and subject matter. (c) Proof of identity. Requests received by mail,...

  20. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... and envelope or any fax cover sheet should be clearly marked “Privacy Act Request.” A Privacy Act... mail or by fax, or delivered in person, a Privacy Act request will not be considered to have been... or name, author, recipient, and subject matter. (c) Proof of identity. Requests received by mail,...

  1. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and envelope or any fax cover sheet should be clearly marked “Privacy Act Request.” A Privacy Act... mail or by fax, or delivered in person, a Privacy Act request will not be considered to have been... or name, author, recipient, and subject matter. (c) Proof of identity. Requests received by mail,...

  2. 76 FR 72914 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Government...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition... Foreign Disclosure and Technical Information System,'' and is used when directed by the plant clearance... Regulations System, Department of Defense (DoD). ACTION: Notice and request for comments. SUMMARY:...

  3. 75 FR 68333 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DFARS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-05

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition...-0248) AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and request for comments regarding a proposed extension of an approved information collection...

  4. 75 FR 41161 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 251...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition... vehicles under the Interagency Fleet Management System (IFMS) and obtain related services. The information... Regulations System, Department of Defense (DoD). ACTION: Notice and request for comments regarding a...

  5. 76 FR 35424 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-17

    ... Regulation Supplement; Acquisition of Information Technology AGENCY: Defense Acquisition Regulations System... technology. The Office of Management and Budget (OMB) has approved this information collection requirement... Supplement (DFARS) Part 239, Acquisition of Information Technology, and the associated clauses at DFARS...

  6. 78 FR 63462 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-24

    ... organizational conflicts of interest arising in a systems engineering and technical assistance contract for an... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition...: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and request...

  7. Federal environmental and occupational toxicology regulations and reporting requirements: a practical approach to what the medical toxicologist needs to know, part 2.

    PubMed

    Schwartz, Michael D; Dell'Aglio, Damon M; Nickle, Richard; Hornsby-Myers, Jennifer

    2014-12-01

    Toxicologists are often called upon to assist in environmental, industrial, occupational and public health assessments. Accordingly, medical toxicologists may find it prudent to be aware of applicable federal toxicological regulations and reporting requirements and of the roles of relevant federal agencies. These regulations are numerous, complex, and have evolved and expanded over time, making it difficult for toxicologists to sustain a current knowledge base. This article reviews the pertinent federal toxicological reporting requirements with regards to the Toxic Substances Control Act (TSCA), the Atomic Energy Act (AEA), the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA), the Resource Conservation and Recovery Act (RCRA), the Clean Air Act, the Clean Water Act, the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), the Emergency Planning and Community Right to Know Act (EPCRA), the Occupational Safety and Health Act, the Department of Transportation, and information about the National Response Center. We reference internet-based government resources and offer direct links to applicable websites in an attempt to offer rapid and current sources of practical information. The format of the article is a series of hypothetical scenarios followed by commentary. Discussions of the Safe Drinking Water Act and the Food, Drug, and Cosmetic Act and the Dietary Supplement Health and Education Act are beyond the scope of this paper. For those desiring a more in depth discussion of the relevant federal environmental laws and statutes, and applicable case law, the reader is directed to resources such as the Environmental Law Handbook, the websites of individual laws found at www.epa.gov and the decisions of individual courts of appeal. It is our hope that this article provides not only useful practical information for the practicing toxicologist, but also serves as a key reference for Medical Toxicology core content on environmental

  8. Federal environmental and occupational toxicology regulations and reporting requirements: a practical approach to what the medical toxicologist needs to know, part 1.

    PubMed

    Schwartz, Michael D; Dell'Aglio, Damon M; Nickle, Richard; Hornsby-Myers, Jennifer

    2014-09-01

    Toxicologists are often called upon to assist in environmental, industrial, occupational and public health assessments. Accordingly, medical toxicologists may find it prudent to be aware of applicable federal toxicological regulations and reporting requirements and of the roles of relevant federal agencies. These regulations are numerous, complex, and have evolved and expanded over time, making it difficult for toxicologists to sustain a current knowledge base. This article reviews the pertinent federal toxicological reporting requirements with regard to the Toxic Substances Control Act (TSCA), the Atomic Energy Act (AEA), the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA), the Resource Conservation and Recovery Act (RCRA), the Clean Air Act, the Clean Water Act, the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), the Emergency Planning and Community Right to Know Act (EPCRA), the Occupational Safety and Health Act, the Department of Transportation, and information about the National Response Center. We reference internet-based government resources and offer direct links to applicable websites in an attempt to offer rapid and current sources of practical information. The format of the article is a series of hypothetical scenarios followed by commentary. Discussions of the Safe Drinking Water Act, the Food, Drug, and Cosmetic Act, and the Dietary Supplement Health and Education Act are beyond the scope of this paper. For those desiring a more in-depth discussion of the relevant federal environmental laws and statutes and applicable case law, the reader is directed to resources such as the Environmental Law Handbook, the websites of individual laws found at www.epa.gov and the decisions of individual courts of appeal. It is our hope that this article provides not only useful practical information for the practicing toxicologist but also serves as a key reference for medical toxicology core content on environmental laws and

  9. PKR is activated by cellular dsRNAs during mitosis and acts as a mitotic regulator

    PubMed Central

    Kim, Yoosik; Lee, Jung Hyun; Park, Jong-Eun; Cho, Jun; Yi, Hyerim; Kim, V. Narry

    2014-01-01

    dsRNA-dependent protein kinase R (PKR) is a ubiquitously expressed enzyme well known for its roles in immune response. Upon binding to viral dsRNA, PKR undergoes autophosphorylation, and the phosphorylated PKR (pPKR) regulates translation and multiple signaling pathways in infected cells. Here, we found that PKR is activated in uninfected cells, specifically during mitosis, by binding to dsRNAs formed by inverted Alu repeats (IRAlus). While PKR and IRAlu-containing RNAs are segregated in the cytosol and nucleus of interphase cells, respectively, they interact during mitosis when nuclear structure is disrupted. Once phosphorylated, PKR suppresses global translation by phosphorylating the α subunit of eukaryotic initiation factor 2 (eIF2α). In addition, pPKR acts as an upstream kinase for c-Jun N-terminal kinase and regulates the levels of multiple mitotic factors such as CYCLINS A and B and POLO-LIKE KINASE 1 and phosphorylation of HISTONE H3. Disruption of PKR activation via RNAi or expression of a transdominant-negative mutant leads to misregulation of the mitotic factors, delay in mitotic progression, and defects in cytokinesis. Our study unveils a novel function of PKR and endogenous dsRNAs as signaling molecules during the mitosis of uninfected cells. PMID:24939934

  10. Gamma-aminobutyric acid acts as a specific virulence regulator in Pseudomonas aeruginosa.

    PubMed

    Dagorn, Audrey; Hillion, Mélanie; Chapalain, Annelise; Lesouhaitier, Olivier; Duclairoir Poc, Cécile; Vieillard, Julien; Chevalier, Sylvie; Taupin, Laure; Le Derf, Franck; Feuilloley, Marc G J

    2013-02-01

    Gamma-aminobutyric acid (GABA) is widespread in the environment and can be used by animal and plants as a communication molecule. Pseudomonas species, in particular fluorescent ones, synthesize GABA and express GABA-binding proteins. In this study, we investigated the effects of GABA on the virulence of Pseudomonas aeruginosa. While exposure to GABA (10 µM) did not modify either the growth kinetics or the motility of the bacterium, its cytotoxicity and virulence were strongly increased. The Caenorhabditis elegans 'fast killing test' model revealed that GABA acts essentially through an increase in diffusible toxin(s). GABA also modulates the biofilm formation activity and adhesion properties of PAO1. GABA has no effect on cell surface polarity, biosurfactant secretion or on the lipopolysaccharide structure. The production of several exo-enzymes, pyoverdin and exotoxin A is not modified by GABA but we observed an increase in cyanogenesis which, by itself, could explain the effect of GABA on P. aeruginosa virulence. This mechanism appears to be regulated by quorum sensing. A proteomic analysis revealed that the effect of GABA on cyanogenesis is correlated with a reduction of oxygen accessibility and an over-expression of oxygen-scavenging proteins. GABA also promotes specific changes in the expression of thermostable and unstable elongation factors Tuf/Ts involved in the interaction of the bacterium with the host proteins. Taken together, these results suggest that GABA is a physiological regulator of P. aeruginosa virulence. PMID:23154974

  11. Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis.

    PubMed

    Prévost, Gaëtan; Jeandel, Lydie; Arabo, Arnaud; Coëffier, Moïse; El Ouahli, Mariama; Picot, Marie; Alexandre, David; Gobet, Françoise; Leprince, Jérôme; Berrahmoune, Hind; Déchelotte, Pierre; Malagon, Maria; Bonner, Caroline; Kerr-Conte, Julie; Chigr, Fatiha; Lefebvre, Hervé; Anouar, Youssef; Chartrel, Nicolas

    2015-08-01

    26RFa is a hypothalamic neuropeptide that promotes food intake. 26RFa is upregulated in obese animal models, and its orexigenic activity is accentuated in rodents fed a high-fat diet, suggesting that this neuropeptide might play a role in the development and maintenance of the obese status. As obesity is frequently associated with type 2 diabetes, we investigated whether 26RFa may be involved in the regulation of glucose homeostasis. In the current study, we show a moderate positive correlation between plasma 26RFa levels and plasma insulin in patients with diabetes. Plasma 26RFa concentration also increases in response to an oral glucose tolerance test. In addition, we found that 26RFa and its receptor GPR103 are present in human pancreatic β-cells as well as in the gut. In mice, 26RFa attenuates the hyperglycemia induced by a glucose load, potentiates insulin sensitivity, and increases plasma insulin concentrations. Consistent with these data, 26RFa stimulates insulin production by MIN6 insulinoma cells. Finally, we show, using in vivo and in vitro approaches, that a glucose load induces a massive secretion of 26RFa by the small intestine. Altogether, the present data indicate that 26RFa acts as an incretin to regulate glucose homeostasis. PMID:25858563

  12. Neuropeptide Y acts in the paraventricular nucleus to suppress sympathetic nerve activity and its baroreflex regulation.

    PubMed

    Cassaglia, Priscila A; Shi, Zhigang; Li, Baoxin; Reis, Wagner L; Clute-Reinig, Nicholas M; Stern, Javier E; Brooks, Virginia L

    2014-04-01

    Neuropeptide Y (NPY), a brain neuromodulator that has been strongly implicated in the regulation of energy balance, also acts centrally to inhibit sympathetic nerve activity (SNA); however, the site and mechanism of action are unknown. In chloralose-anaesthetized female rats, nanoinjection of NPY into the paraventricular nucleus of the hypothalamus (PVN) dose-dependently suppressed lumbar SNA (LSNA) and its baroreflex regulation, and these effects were blocked by prior inhibition of NPY Y1 or Y5 receptors. Moreover, PVN injection of Y1 and Y5 receptor antagonists in otherwise untreated rats increased basal and baroreflex control of LSNA, indicating that endogenous NPY tonically inhibits PVN presympathetic neurons. The sympathoexcitation following blockade of PVN NPY inhibition was eliminated by prior PVN nanoinjection of the melanocortin 3/4 receptor inhibitor SHU9119. Moreover, presympathetic neurons, identified immunohistochemically using cholera toxin b neuronal tract tracing from the rostral ventrolateral medulla (RVLM), express NPY Y1 receptor immunoreactivity, and patch-clamp recordings revealed that both NPY and α-melanocyte-stimulating hormone (α-MSH) inhibit and stimulate, respectively, PVN-RVLM neurons. Collectively, these data suggest that PVN NPY inputs converge with α-MSH to influence presympathetic neurons. Together these results identify endogenous NPY as a novel and potent inhibitory neuromodulator within the PVN that may contribute to changes in SNA that occur in states associated with altered energy balance, such as obesity and pregnancy. PMID:24535439

  13. Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis

    NASA Technical Reports Server (NTRS)

    Brown, D. E.; Rashotte, A. M.; Murphy, A. S.; Normanly, J.; Tague, B. W.; Peer, W. A.; Taiz, L.; Muday, G. K.

    2001-01-01

    Polar transport of the plant hormone auxin controls many aspects of plant growth and development. A number of synthetic compounds have been shown to block the process of auxin transport by inhibition of the auxin efflux carrier complex. These synthetic auxin transport inhibitors may act by mimicking endogenous molecules. Flavonoids, a class of secondary plant metabolic compounds, have been suggested to be auxin transport inhibitors based on their in vitro activity. The hypothesis that flavonoids regulate auxin transport in vivo was tested in Arabidopsis by comparing wild-type (WT) and transparent testa (tt4) plants with a mutation in the gene encoding the first enzyme in flavonoid biosynthesis, chalcone synthase. In a comparison between tt4 and WT plants, phenotypic differences were observed, including three times as many secondary inflorescence stems, reduced plant height, decreased stem diameter, and increased secondary root development. Growth of WT Arabidopsis plants on naringenin, a biosynthetic precursor to those flavonoids with auxin transport inhibitor activity in vitro, leads to a reduction in root growth and gravitropism, similar to the effects of synthetic auxin transport inhibitors. Analyses of auxin transport in the inflorescence and hypocotyl of independent tt4 alleles indicate that auxin transport is elevated in plants with a tt4 mutation. In hypocotyls of tt4, this elevated transport is reversed when flavonoids are synthesized by growth of plants on the flavonoid precursor, naringenin. These results are consistent with a role for flavonoids as endogenous regulators of auxin transport.

  14. Neuropeptide Y acts in the paraventricular nucleus to suppress sympathetic nerve activity and its baroreflex regulation

    PubMed Central

    Cassaglia, Priscila A; Shi, Zhigang; Li, Baoxin; Reis, Wagner L; Clute-Reinig, Nicholas M; Stern, Javier E; Brooks, Virginia L

    2014-01-01

    Neuropeptide Y (NPY), a brain neuromodulator that has been strongly implicated in the regulation of energy balance, also acts centrally to inhibit sympathetic nerve activity (SNA); however, the site and mechanism of action are unknown. In chloralose-anaesthetized female rats, nanoinjection of NPY into the paraventricular nucleus of the hypothalamus (PVN) dose-dependently suppressed lumbar SNA (LSNA) and its baroreflex regulation, and these effects were blocked by prior inhibition of NPY Y1 or Y5 receptors. Moreover, PVN injection of Y1 and Y5 receptor antagonists in otherwise untreated rats increased basal and baroreflex control of LSNA, indicating that endogenous NPY tonically inhibits PVN presympathetic neurons. The sympathoexcitation following blockade of PVN NPY inhibition was eliminated by prior PVN nanoinjection of the melanocortin 3/4 receptor inhibitor SHU9119. Moreover, presympathetic neurons, identified immunohistochemically using cholera toxin b neuronal tract tracing from the rostral ventrolateral medulla (RVLM), express NPY Y1 receptor immunoreactivity, and patch-clamp recordings revealed that both NPY and α–melanocyte-stimulating hormone (α-MSH) inhibit and stimulate, respectively, PVN–RVLM neurons. Collectively, these data suggest that PVN NPY inputs converge with α-MSH to influence presympathetic neurons. Together these results identify endogenous NPY as a novel and potent inhibitory neuromodulator within the PVN that may contribute to changes in SNA that occur in states associated with altered energy balance, such as obesity and pregnancy. PMID:24535439

  15. Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis.

    PubMed

    Brown, D E; Rashotte, A M; Murphy, A S; Normanly, J; Tague, B W; Peer, W A; Taiz, L; Muday, G K

    2001-06-01

    Polar transport of the plant hormone auxin controls many aspects of plant growth and development. A number of synthetic compounds have been shown to block the process of auxin transport by inhibition of the auxin efflux carrier complex. These synthetic auxin transport inhibitors may act by mimicking endogenous molecules. Flavonoids, a class of secondary plant metabolic compounds, have been suggested to be auxin transport inhibitors based on their in vitro activity. The hypothesis that flavonoids regulate auxin transport in vivo was tested in Arabidopsis by comparing wild-type (WT) and transparent testa (tt4) plants with a mutation in the gene encoding the first enzyme in flavonoid biosynthesis, chalcone synthase. In a comparison between tt4 and WT plants, phenotypic differences were observed, including three times as many secondary inflorescence stems, reduced plant height, decreased stem diameter, and increased secondary root development. Growth of WT Arabidopsis plants on naringenin, a biosynthetic precursor to those flavonoids with auxin transport inhibitor activity in vitro, leads to a reduction in root growth and gravitropism, similar to the effects of synthetic auxin transport inhibitors. Analyses of auxin transport in the inflorescence and hypocotyl of independent tt4 alleles indicate that auxin transport is elevated in plants with a tt4 mutation. In hypocotyls of tt4, this elevated transport is reversed when flavonoids are synthesized by growth of plants on the flavonoid precursor, naringenin. These results are consistent with a role for flavonoids as endogenous regulators of auxin transport. PMID:11402184

  16. Decree No. 0784 of 18 April 1989 regulating, in part, Act 21 of 1982 and Act 71 of 1988.

    PubMed

    1989-01-01

    This Colombian Decree regulates certain aspects of the system of family allowances. Among other things, it provides that allowances can be granted in terms of food, clothing, school scholarships, textbooks, drugs, and other substitutes for money. Further provisions of the Decree deal with the definition of persons affiliated to the regime, booklets certifying affiliation, family service subsidies, and financing. PMID:12344547

  17. Two Group A Streptococcal Peptide Pheromones Act through Opposing Rgg Regulators to Control Biofilm Development

    PubMed Central

    Chang, Jennifer C.; LaSarre, Breah; Jimenez, Juan C.; Aggarwal, Chaitanya; Federle, Michael J.

    2011-01-01

    Streptococcus pyogenes (Group A Streptococcus, GAS) is an important human commensal that occasionally causes localized infections and less frequently causes severe invasive disease with high mortality rates. How GAS regulates expression of factors used to colonize the host and avoid immune responses remains poorly understood. Intercellular communication is an important means by which bacteria coordinate gene expression to defend against host assaults and competing bacteria, yet no conserved cell-to-cell signaling system has been elucidated in GAS. Encoded within the GAS genome are four rgg-like genes, two of which (rgg2 and rgg3) have no previously described function. We tested the hypothesis that rgg2 or rgg3 rely on extracellular peptides to control target-gene regulation. We found that Rgg2 and Rgg3 together tightly regulate two linked genes encoding new peptide pheromones. Rgg2 activates transcription of and is required for full induction of the pheromone genes, while Rgg3 plays an antagonistic role and represses pheromone expression. The active pheromone signals, termed SHP2 and SHP3, are short and hydrophobic (DI[I/L]IIVGG), and, though highly similar in sequence, their ability to disrupt Rgg3-DNA complexes were observed to be different, indicating that specificity and differential activation of promoters are characteristics of the Rgg2/3 regulatory circuit. SHP-pheromone signaling requires an intact oligopeptide permease (opp) and a metalloprotease (eep), supporting the model that pro-peptides are secreted, processed to the mature form, and subsequently imported to the cytoplasm to interact directly with the Rgg receptors. At least one consequence of pheromone stimulation of the Rgg2/3 pathway is increased biogenesis of biofilms, which counteracts negative regulation of biofilms by RopB (Rgg1). These data provide the first demonstration that Rgg-dependent quorum sensing functions in GAS and substantiate the role that Rggs play as peptide receptors across the

  18. 78 FR 25795 - Contractor Legal Management Requirements; Acquisition Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-03

    ... Federal Register on August 31, 1994 (59 FR 44981). The interim Acquisition Letter was finalized as a Policy Statement on April 3, 1996 (61 FR 14763). This Policy Statement was followed by a formal... Regulations with an effective date of April 23, 2001 (66 FR 4616, 66 FR 19717). After a decade operating...

  19. 77 FR 4498 - Rules and Regulations Under the Wool Products Labeling Act of 1939

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-30

    ... Identification Act, the Wool Products Labeling Act, and the Fur Products Labeling Act: Final Rule, 63 FR 7508...: Miscellaneous Rules: Final Rule, 63 FR 71582 (Dec. 29, 1998). \\7\\ Federal Trade Commission: Rules and... Labeling Act of 1939, Final Rule, 65 FR 75154 (Dec. 1, 2000). \\8\\ The Wool Rules provide that...

  20. Intergovernmental relations inherent in the Energy Management Partnership Act: a workshop on information requirements

    SciTech Connect

    Hoop, W.J.; Edelson, E.

    1980-02-01

    This paper summarizes the results of the first of three workshops that were planned to assess the information needed by the Office of Conservation and Solar Energy (CS) to effectively evaluate the pending Energy Management Partnership Act (EMPA); the workshop concentrated on issues of the EMPA hierarchical partnership. The approach utilized offers two major benefits to CS. First, by considering the problem of program evaluation while EMPA is still in the planning stage, this study identifies any baseline information that should be collected prior to implementation of EMPA, and also provides CS with the opportunity to include evaluation considerations in the operating guidelines for the program. Second, by identifying the potential problems and benefits inherent in EMPA and then identifying the information necessary to evaluate these problems and benefits, information requirements tied to the reasons for needing that information are generated, rather than a long unrelated laundry list of information requirements. Drafting of EMPA is not yet complete. When the term EMPA is used here, it refers to a set of bills that are presently being melded together. The original EMPA bill, which originated in DOE, was designed to expand the role of state and local governments in achieving national energy goals. Specifically, EMPA would provide a total of $110 million annually to state and local governments over a five year period to (1) develop an overall state energy plan, (2) consolidate three existing federal energy grant programs, (3) allow the secretary to fund directly innovative projects at the local level, and (4) provide additional assistance to states to cover the administrative costs of existing energy programs. Other bills, which may be passed in conjuncttion with EMPA or incorporated into EMPA, place additional emphasis on the local level by allocating as much as $400 million annually to local governments.

  1. Transcriptional Regulation via Nuclear Receptor Crosstalk Required for the Drosophila Circadian Clock

    PubMed Central

    Jaumouillé, Edouard; Machado Almeida, Pedro; Stähli, Patrick; Koch, Rafael; Nagoshi, Emi

    2015-01-01

    Summary Circadian clocks in large part rely on transcriptional feedback loops. At the core of the clock machinery, the transcriptional activators CLOCK/BMAL1 (in mammals) and CLOCK/CYCLE (CLK/CYC) (in Drosophila) drive the expression of the period (per) family genes. The PER-containing complexes inhibit the activity of CLOCK/BMAL1 or CLK/CYC, thereby forming a negative feedback loop [1]. In mammals, the ROR and REV-ERB family nuclear receptors add positive and negative transcriptional regulation to this core negative feedback loop to ensure the generation of robust circadian molecular oscillation [2]. Despite the overall similarities between mammalian and Drosophila clocks, whether comparable mechanisms via nuclear receptors are required for the Drosophila clock remains unknown. We show here that the nuclear receptor E75, the fly homolog of REV-ERB α and REV-ERB β, and the NR2E3 subfamily nuclear receptor UNF are components of the molecular clocks in the Drosophila pacemaker neurons. In vivo assays in conjunction with the in vitro experiments demonstrate that E75 and UNF bind to per regulatory sequences and act together to enhance the CLK/CYC-mediated transcription of the per gene, thereby completing the core transcriptional feedback loop necessary for the free-running clockwork. Our results identify a missing link in the Drosophila clock and highlight the significance of the transcriptional regulation via nuclear receptors in metazoan circadian clocks. PMID:26004759

  2. Transcriptional regulation via nuclear receptor crosstalk required for the Drosophila circadian clock.

    PubMed

    Jaumouillé, Edouard; Machado Almeida, Pedro; Stähli, Patrick; Koch, Rafael; Nagoshi, Emi

    2015-06-01

    Circadian clocks in large part rely on transcriptional feedback loops. At the core of the clock machinery, the transcriptional activators CLOCK/BMAL1 (in mammals) and CLOCK/CYCLE (CLK/CYC) (in Drosophila) drive the expression of the period (per) family genes. The PER-containing complexes inhibit the activity of CLOCK/BMAL1 or CLK/CYC, thereby forming a negative feedback loop [1]. In mammals, the ROR and REV-ERB family nuclear receptors add positive and negative transcriptional regulation to this core negative feedback loop to ensure the generation of robust circadian molecular oscillation [2]. Despite the overall similarities between mammalian and Drosophila clocks, whether comparable mechanisms via nuclear receptors are required for the Drosophila clock remains unknown. We show here that the nuclear receptor E75, the fly homolog of REV-ERB α and REV-ERB β, and the NR2E3 subfamily nuclear receptor UNF are components of the molecular clocks in the Drosophila pacemaker neurons. In vivo assays in conjunction with the in vitro experiments demonstrate that E75 and UNF bind to per regulatory sequences and act together to enhance the CLK/CYC-mediated transcription of the per gene, thereby completing the core transcriptional feedback loop necessary for the free-running clockwork. Our results identify a missing link in the Drosophila clock and highlight the significance of the transcriptional regulation via nuclear receptors in metazoan circadian clocks. PMID:26004759

  3. Integrating NEPA (National Environmental Policy Act) and CERCLA (Comprehensive Environmental Response, Compensation, and Liability Act) requirements during remedial responses at DOE facilities

    SciTech Connect

    Levine, M.B.; Smith, E.D.; Sharples, F.E.; Eddlemon, G.K.

    1990-07-01

    US Department of Energy (DOE) Order 5400.4, issued October 6, 1989, calls for integrating the requirements of the National Environmental Policy Act (NEPA) with those of the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) for DOE remedial actions under CERCLA. CERCLA requires that decisions on site remediation be made through a formal process called a Remedial Investigation/Feasibility Study (RI/FS). According to the DOE order, integration is to be accomplished by conducting the NEPA and CERCLA environmental planning and review procedures concurrently. The primary instrument for integrating the processes is to be the RI/FS process, which will be supplemented as needed to meet the procedural and documentational requirements of NEPA. The final product of the integrated process will be a single, integrated set of documents; namely, an RI report and an FS-EIS that satisfy the requirements of both NEPA and CERCLA. The contents of the report include (1) an overview and comparison of the requirements of the two processes; (2) descriptions of the major tasks included in the integrated RI/FS-EIS process; (3) recommended contents for integrated RI/FS-EIS documents; and (4)a discussion of some potential problems in integrating NEPA and CERCLA that fall outisde the scope of the RI/FS-EIS process, with suggestions for resolving some of these problems. 15 refs.

  4. Polyadenylation in Rice Tungro Bacilliform Virus: cis-Acting Signals and Regulation

    PubMed Central

    Rothnie, Helen M.; Chen, Gang; Fütterer, Johannes; Hohn, Thomas

    2001-01-01

    The polyadenylation signal of rice tungro bacilliform virus (RTBV) was characterized by mutational and deletion analysis. The cis-acting signals required to direct polyadenylation conformed to what is known for plant poly(A) signals in general and were very similar to those of the related cauliflower mosaic virus. Processing was directed by a canonical AAUAAA poly(A) signal, an upstream UG-rich region considerably enhanced processing efficiency, and sequences downstream of the cleavage site were not required. When present at the end of a transcription unit, the cis-acting signals for 3′-end processing were highly efficient in both monocot (rice) and dicot (Nicotiana plumbaginifolia) protoplasts. In a promoter-proximal position, as in the viral genome, the signal was also efficiently processed in rice protoplasts, giving rise to an abundant “short-stop” (SS-) RNA. The proportion of SS-RNA was considerably lower in N. plumbaginifolia protoplasts. In infected plants, SS-RNA was hardly detectable, suggesting either that SS-RNA is unstable in infected plants or that read-through of the promoter-proximal poly(A) site is very efficient. SS-RNA is readily detectable in transgenic rice plants (A. Klöti, C. Henrich, S. Bieri, X. He, G. Chen, P. K. Burkhardt, J. Wünn, P. Lucca, T. Hohn, I. Potrylus, and J. Fütterer, 1999. Plant Mol. Biol. 40:249–266), thus the absence of SS-RNA in infected plants can be attributed to poly(A) site bypass in the viral context to ensure production of the full-length pregenomic viral RNA. RTBV poly(A) site suppression thus depends both on context and the expression system; our results suggest that the circular viral minichromosome directs assembly of a transcription-processing complex with specific properties to effect read-through of the promoter-proximal poly(A) signal. PMID:11287568

  5. Cholesterol and regulated exocytosis: a requirement for unitary exocytotic events.

    PubMed

    Rituper, Boštjan; Flašker, Ajda; Guček, Alenka; Chowdhury, Helena H; Zorec, Robert

    2012-01-01

    Since the 1970s, much effort was been expended researching mechanisms of regulated exocytosis. Early work focused mainly on the role of proteins. Most notably the discovery of SNARE proteins in the 1980s and the zippering hypothesis brought us much closer to understanding the complex interactions in membrane fusion between vesicle and plasma membranes, a pivotal component of regulated exocytosis. However, most likely due to the predictions of the Singer-Nicholson fluid mosaic membrane model, the lipid components of the exocytotic machinery remained largely overlooked. Lipids were considered passive constituents of cellular membranes, not contributing much, if anything, to the process of exocytosis and membrane fusion. Since the 1990s, this so-called proteocentric view has been gradually giving way to the new perspective best described with the term proteolipidic. Many lipids were found to be of great importance in the regulation of exocytosis. Here we highlight the role of cholesterol. Furthermore, by using high-resolution cell-attached membrane capacitance measurements, we have monitored unitary exocytotic events in cholesterol-depleted membranes. We show that the frequency of these events is attenuated, providing evidence at the single vesicle level that cholesterol directly influences the merger of the vesicle and the plasma membranes. PMID:22726879

  6. The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling1

    PubMed Central

    Wu, Ling; Wang, Chenhui; Boisson, Bertrand; Misra, Saurav; Rayman, Patricia; Finke, James H.; Puel, Anne; Casanova, Jean-Laurent; Li, Xiaoxia

    2014-01-01

    IL-17 is a pro-inflammatory cytokine implicated in the pathogenesis of autoimmune diseases including psoriasis. ACT1 is an essential adaptor molecule in the IL-17 signaling pathway. A missense single nucleotide polymorphism (rs33980500; SNP-D10N) that resulted in the substitution of an asparagine for an aspartic acid at position 10 of ACT1 (ACT1-D10N) is associated with psoriasis susceptibility. Due to alternative splicing in humans, SNP-D10N encodes two mutated ACT1 proteins, ACT1-D10N and ACT1-D19N. Though both ACT1 isoforms are Hsp90 ‘client’ proteins, the nine additional amino acids in ACT1-D19N provide an additional Hsp90 binding site that is absent in ACT1-D10N. Therefore, while ACT1-D10N is a dead protein that is unable to transduce IL-17 signals for gene expression, ACT1-D19N is fully responsive to IL-17. Intriguingly, the two ACT1 isoforms are differentially expressed in ACT1D10N/D10N fibroblasts and T cells. Fibroblasts express both isoforms equally, enabling ACT1-D19N to compensate for the loss of ACT1-D10N function. ACT1D10N/D10N T cells, however, express predominantly ACT1-D10N. Lacking this compensatory mechanism, ACT1D10N/D10N T cells behave like ACT1-deficient T cells, exhibiting a dysregulated and hyperactive Th17 phenotype with overproduction of IL-22 and IL-17. The hyperactive Th17 response combined with fully responsive fibroblasts likely synergized to contribute to psoriasis susceptibility in SNP-D10N patients. PMID:25024377

  7. The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling.

    PubMed

    Wu, Ling; Wang, Chenhui; Boisson, Bertrand; Misra, Saurav; Rayman, Patricia; Finke, James H; Puel, Anne; Casanova, Jean-Laurent; Li, Xiaoxia

    2014-08-15

    IL-17 is a proinflammatory cytokine implicated in the pathogenesis of autoimmune diseases including psoriasis. ACT1 is an essential adaptor molecule in the IL-17 signaling pathway. A missense single nucleotide polymorphism (rs33980500; SNP-D10N) that resulted in the substitution of an asparagine for an aspartic acid at position 10 of ACT1 (ACT1-D10N) is associated with psoriasis susceptibility. Due to alternative splicing in humans, SNP-D10N encodes two mutated ACT1 proteins, ACT1-D10N and ACT1-D19N. Although both ACT1 isoforms are Hsp90 client proteins, the nine additional amino acids in ACT1-D19N provide an additional Hsp90 binding site that is absent in ACT1-D10N. Therefore, whereas ACT1-D10N is a dead protein that is unable to transduce IL-17 signals for gene expression, ACT1-D19N is fully responsive to IL-17. Intriguingly, the two ACT1 isoforms are differentially expressed in ACT1(D10N/D10N) fibroblasts and T cells. Fibroblasts express both isoforms equally, enabling ACT1-D19N to compensate for the loss of ACT1-D10N function. ACT1(D10N/D10N) T cells, however, express predominantly ACT1-D10N. Lacking this compensatory mechanism, ACT1(D10N/D10N) T cells behave like ACT1-deficient T cells, exhibiting a dysregulated and hyperactive Th17 phenotype with overproduction of IL-22 and IL-17. The hyperactive Th17 response combined with fully responsive fibroblasts likely synergized to contribute to psoriasis susceptibility in SNP-D10N patients. PMID:25024377

  8. Phosphorylation acts positively and negatively to regulate MRTF-A subcellular localisation and activity.

    PubMed

    Panayiotou, Richard; Miralles, Francesc; Pawlowski, Rafal; Diring, Jessica; Flynn, Helen R; Skehel, Mark; Treisman, Richard

    2016-01-01

    The myocardin-related transcription factors (MRTF-A and MRTF-B) regulate cytoskeletal genes through their partner transcription factor SRF. The MRTFs bind G-actin, and signal-regulated changes in cellular G-actin concentration control their nuclear accumulation. The MRTFs also undergo Rho- and ERK-dependent phosphorylation, but the function of MRTF phosphorylation, and the elements and signals involved in MRTF-A nuclear export are largely unexplored. We show that Rho-dependent MRTF-A phosphorylation reflects relief from an inhibitory function of nuclear actin. We map multiple sites of serum-induced phosphorylation, most of which are S/T-P motifs and show that S/T-P phosphorylation is required for transcriptional activation. ERK-mediated S98 phosphorylation inhibits assembly of G-actin complexes on the MRTF-A regulatory RPEL domain, promoting nuclear import. In contrast, S33 phosphorylation potentiates the activity of an autonomous Crm1-dependent N-terminal NES, which cooperates with five other NES elements to exclude MRTF-A from the nucleus. Phosphorylation thus plays positive and negative roles in the regulation of MRTF-A. PMID:27304076

  9. Phosphorylation acts positively and negatively to regulate MRTF-A subcellular localisation and activity

    PubMed Central

    Panayiotou, Richard; Miralles, Francesc; Pawlowski, Rafal; Diring, Jessica; Flynn, Helen R; Skehel, Mark; Treisman, Richard

    2016-01-01

    The myocardin-related transcription factors (MRTF-A and MRTF-B) regulate cytoskeletal genes through their partner transcription factor SRF. The MRTFs bind G-actin, and signal-regulated changes in cellular G-actin concentration control their nuclear accumulation. The MRTFs also undergo Rho- and ERK-dependent phosphorylation, but the function of MRTF phosphorylation, and the elements and signals involved in MRTF-A nuclear export are largely unexplored. We show that Rho-dependent MRTF-A phosphorylation reflects relief from an inhibitory function of nuclear actin. We map multiple sites of serum-induced phosphorylation, most of which are S/T-P motifs and show that S/T-P phosphorylation is required for transcriptional activation. ERK-mediated S98 phosphorylation inhibits assembly of G-actin complexes on the MRTF-A regulatory RPEL domain, promoting nuclear import. In contrast, S33 phosphorylation potentiates the activity of an autonomous Crm1-dependent N-terminal NES, which cooperates with five other NES elements to exclude MRTF-A from the nucleus. Phosphorylation thus plays positive and negative roles in the regulation of MRTF-A. DOI: http://dx.doi.org/10.7554/eLife.15460.001 PMID:27304076

  10. 78 FR 68831 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-15

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition... System, Department of Defense (DoD). ACTION: Notice and request for comments regarding a proposed extension of an approved information collection requirement. SUMMARY: In compliance with Section...

  11. 78 FR 67133 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-08

    ... holders further disseminate the information to other firms within a geographic area defined in the... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition... approved information collection requirement. SUMMARY: In compliance with Section 3506(c)(2)(A) of...

  12. Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis.

    PubMed

    Shi, Bihai; Zhang, Cui; Tian, Caihuan; Wang, Jin; Wang, Quan; Xu, Tengfei; Xu, Yan; Ohno, Carolyn; Sablowski, Robert; Heisler, Marcus G; Theres, Klaus; Wang, Ying; Jiao, Yuling

    2016-07-01

    Shoot branching requires the establishment of new meristems harboring stem cells; this phenomenon raises questions about the precise regulation of meristematic fate. In seed plants, these new meristems initiate in leaf axils to enable lateral shoot branching. Using live-cell imaging of leaf axil cells, we show that the initiation of axillary meristems requires a meristematic cell population continuously expressing the meristem marker SHOOT MERISTEMLESS (STM). The maintenance of STM expression depends on the leaf axil auxin minimum. Ectopic expression of STM is insufficient to activate axillary buds formation from plants that have lost leaf axil STM expressing cells. This suggests that some cells undergo irreversible commitment to a developmental fate. In more mature leaves, REVOLUTA (REV) directly up-regulates STM expression in leaf axil meristematic cells, but not in differentiated cells, to establish axillary meristems. Cell type-specific binding of REV to the STM region correlates with epigenetic modifications. Our data favor a threshold model for axillary meristem initiation, in which low levels of STM maintain meristematic competence and high levels of STM lead to meristem initiation. PMID:27398935

  13. Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis

    PubMed Central

    Tian, Caihuan; Wang, Jin; Xu, Tengfei; Xu, Yan; Ohno, Carolyn; Sablowski, Robert; Heisler, Marcus G.; Theres, Klaus; Wang, Ying

    2016-01-01

    Shoot branching requires the establishment of new meristems harboring stem cells; this phenomenon raises questions about the precise regulation of meristematic fate. In seed plants, these new meristems initiate in leaf axils to enable lateral shoot branching. Using live-cell imaging of leaf axil cells, we show that the initiation of axillary meristems requires a meristematic cell population continuously expressing the meristem marker SHOOT MERISTEMLESS (STM). The maintenance of STM expression depends on the leaf axil auxin minimum. Ectopic expression of STM is insufficient to activate axillary buds formation from plants that have lost leaf axil STM expressing cells. This suggests that some cells undergo irreversible commitment to a developmental fate. In more mature leaves, REVOLUTA (REV) directly up-regulates STM expression in leaf axil meristematic cells, but not in differentiated cells, to establish axillary meristems. Cell type-specific binding of REV to the STM region correlates with epigenetic modifications. Our data favor a threshold model for axillary meristem initiation, in which low levels of STM maintain meristematic competence and high levels of STM lead to meristem initiation. PMID:27398935

  14. Alternative 3'UTRs act as scaffolds to regulate membrane protein localization

    PubMed Central

    Berkovits, Binyamin D.; Mayr, Christine

    2015-01-01

    About half of human genes use alternative cleavage and polyadenylation (ApA) to generate mRNA transcripts that differ in the length of their 3' untranslated regions (3'UTRs) while producing the same protein 1–3. Here we show in human cell lines that alternative 3' UTRs differentially regulate the localization of membrane proteins. The long 3'UTR of CD47 enables efficient cell surface expression of CD47 protein, whereas the short 3'UTR primarily localizes CD47 protein to the endoplasmic reticulum. CD47 protein localization occurs post-translationally and independently of RNA localization. In our model of 3' UTR-dependent protein localization, the long 3' UTR of CD47 acts as a scaffold to recruit a protein complex containing the RNA-binding protein HuR (also known as ELAVL1) and SET4 to the site of translation. This facilitates interaction of SET with the newly translated cytoplasmic domains of CD47 and results in subsequent translocation of CD47 to the plasma membrane via activated RAC1 5. We also show that CD47 protein has different functions depending on whether it was generated by the short or long 3'UTR isoforms. Thus, ApA contributes to the functional diversity of the proteome without changing the amino acid sequence. 3' UTR-dependent protein localization has the potential to be a widespread trafficking mechanism for membrane proteins because HuR binds to thousands of mRNAs6–9, and we show that the long 3' UTRs of CD44, ITGA1 and TNFRSF13C, which are bound by HuR, increase surface protein expression compared to their corresponding short 3' UTRs. We propose that during translation the scaffold function of 3' UTRs facilitates binding of proteins to nascent proteins to direct their transport or function—and that this role of 3' UTRs can be regulated by ApA. PMID:25896326

  15. 49 CFR 390.11 - Motor carrier to require observance of driver regulations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Motor carrier to require observance of driver regulations. 390.11 Section 390.11 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS FEDERAL MOTOR CARRIER...

  16. 49 CFR 379.11 - Waiver of requirements of the regulations in this part.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Waiver of requirements of the regulations in this part. 379.11 Section 379.11 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS PRESERVATION OF RECORDS §...

  17. 7 CFR 318.13-3 - General requirements for all regulated articles.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 5 2011-01-01 2011-01-01 false General requirements for all regulated articles. 318.13-3 Section 318.13-3 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE STATE OF HAWAII AND TERRITORIES QUARANTINE NOTICES Regulated Articles From Hawaii and...

  18. 49 CFR 195.11 - What is a regulated rural gathering line and what requirements apply?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false What is a regulated rural gathering line and what...) PIPELINE SAFETY TRANSPORTATION OF HAZARDOUS LIQUIDS BY PIPELINE General § 195.11 What is a regulated rural gathering line and what requirements apply? Each operator of a regulated rural gathering line, as defined...

  19. 77 FR 24611 - Removal of Regulations Requiring 3% Withholding by Government Entities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-25

    ... 9524, 76 FR 26583, 2011-23 IRB 843) (the May 2011 final regulations). Those regulations were issued to... in the Federal Register on May 9, 2011 (REG-151687-10, 76 FR 26678, 2011-23 IRB 867). A related... Internal Revenue Service 26 CFR Part 31 RIN 1545-BK83 Removal of Regulations Requiring 3% Withholding...

  20. 15 CFR Supplement No. 1 to Part 730 - Information Collection Requirements Under the Paperwork Reduction Act: OMB Control Numbers

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND... requirements for the Bureau of Industry and Security by the Office of Management and Budget (OMB), pursuant to...-0026 Short Supply Regulations—Petroleum Products § 754.3. 0694-0027 Short Supply Regulations...

  1. Impact of Clean Air Act Regulations on Nitrogen Fate and Transport in the Neuse River Basin

    NASA Astrophysics Data System (ADS)

    Gabriel, M. C.; Knightes, C. D.; Dennis, R. L.; Cooter, E. J.

    2012-12-01

    This study investigated impacts of Clean Air Act Amendment (CAAA) NOx emissions regulations on the fate and transport of nitrogen for two watersheds in the Neuse River Basin, North Carolina, USA from 1990 to 2020. The Soil and Water Assessment Tool (SWAT) and the Community Multi-scale Air Quality (CMAQ) modeling system were used. CMAQ simulated atmospheric chemical transport and nitrogen deposition. This data was entered into SWAT which simulated watershed hydrology and water quality. Two cases were investigated: one that incorporates CAAA regulatory emissions controls in CMAQ simulation (with) and a second case that does not (without). SWAT model results forecasted a 70% decrease in inorganic nitrogen discharge from the Little River watershed and a 50% decrease for the Nahunta watershed by 2020 under the emission control (with) scenario. Denitrification and plant nitrogen uptake played important roles in nitrogen discharge from each watershed. The nitrogen discharge response time following a change in atmospheric nitrogen deposition was 4 years for the Nahunta watershed and 2 years for the Little River watershed. The longer response time for Nahunta is primarily due to a higher percentage of soybean land cover (22.5% [Nahunta]; 1.6% [Little River]). Agricultural land covers had varied nitrogen response times to changes in atmospheric deposition, particularly for soybean, hay and corn. The studied watersheds retained >80% of all nitrogen delivered by agriculture fertilization, biological fixation and atmospheric deposition.

  2. 76 FR 35218 - Federal Acquisition Regulation; Information Collection; Cost or Pricing Data Requirements and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-16

    ... Regulation; Information Collection; Cost or Pricing Data Requirements and Information Other Than Cost or... requirement concerning cost or pricing data requirements and information other than cost or pricing data... Information Collection 9000- 0013, Cost or Pricing Data Requirements and Information Other Than Cost...

  3. 77 FR 29983 - Federal Acquisition Regulation; Information Collection; Novation/Change of Name Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... Regulation; Information Collection; Novation/ Change of Name Requirements AGENCY: Department of Defense (DOD... collection requirement concerning Novation/Change of Name Requirements. Public comments are particularly... comments identified by Information Collection 9000- 0076, Novation/Change of Name Requirements, by any...

  4. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES... objective intent; (3) The manufacture of the HCT/P does not involve the combination of the cells or tissues... not have a systemic effect and is not dependent upon the metabolic activity of living cells for...

  5. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES... objective intent; (3) The manufacture of the HCT/P does not involve the combination of the cells or tissues... not have a systemic effect and is not dependent upon the metabolic activity of living cells for...

  6. 76 FR 71558 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Requests...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ... Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition... Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and request for comments. SUMMARY...), DoD announces the proposed extension of a public information collection requirement and seeks...

  7. 49 CFR 1220.5 - Waiver of requirements of these regulations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Waiver of requirements of these regulations. 1220.5 Section 1220.5 Transportation Other Regulations Relating to Transportation (Continued) SURFACE TRANSPORTATION BOARD, DEPARTMENT OF TRANSPORTATION (CONTINUED) ACCOUNTS, RECORDS AND REPORTS PRESERVATION OF RECORDS § 1220.5 Waiver of requirements...

  8. 29 CFR 102.117 - Freedom of Information Act Regulations: Board materials and formal documents available for public...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 2 2011-07-01 2011-07-01 false Freedom of Information Act Regulations: Board materials and formal documents available for public inspection and copying; requests for described records; time limit for response; appeal from denial of request; fees for document search and duplication; files and records not subject to inspection....

  9. 75 FR 8844 - Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations-Reports of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY 31 CFR Part 103 RIN 1506-AB08 Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations--Reports of Foreign Financial Accounts AGENCY: Financial Crimes Enforcement Network...

  10. 78 FR 6407 - Ability-to-Repay and Qualified Mortgage Standards Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... underwriting standards. See 73 FR 44524 (July 30, 2008). The following discussion provides background... rulemaking authority by section 1029 of the Dodd- Frank Act). 76 FR 79768 (Dec. 22, 2011). This rule did not... Regulation Z. See 60 FR 15463 (Mar. 24, 1995). In particular, Sec. 226.32(e)(1) \\42\\ implemented TILA...

  11. 78 FR 23171 - Amendments to the 2013 Escrows Final Rule Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-18

    ... loans made in ``rural'' or ``underserved'' areas. \\1\\ 78 FR 4726 (Jan. 22, 2013). \\2\\ The other rules...) (2013 ATR Final Rule), 78 FR 6407; High-Cost Mortgages and Homeownership Counseling Amendments to the... Settlement Procedures Act (Regulation X) (2013 HOEPA Final Rule), 78 FR 6855; Disclosure and...

  12. 75 FR 29526 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission 61,161; Docket No. RM93-11-000] Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods May 19, 2010. The...

  13. 78 FR 60381 - Amendments to the 2013 Mortgage Rules Under the Equal Credit Opportunity Act (Regulation B), Real...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-01

    ... February 15, 2013, at 78 FR 11280. FOR FURTHER INFORMATION CONTACT: Whitney Patross, Attorney; Richard... Under the Truth in Lending Act (Regulation Z), 78 FR 4726 (Jan. 22, 2013) (2013 Escrows Final Rule...), 78 FR 6856 (Jan. 31, 2013) (2013 HOEPA Final Rule), and Ability-to-Repay and Qualified...

  14. 9 CFR 331.3 - States designated under paragraph 301(c) of the Act; application of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false States designated under paragraph 301(c) of the Act; application of regulations. 331.3 Section 331.3 Animals and Animal Products FOOD... DESIGNATED STATES AND TERRITORIES; AND FOR DESIGNATION OF ESTABLISHMENTS WHICH ENDANGER PUBLIC HEALTH AND...

  15. 9 CFR 381.222 - States designated under paragraph 5(c) of the Act; application of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false States designated under paragraph 5(c) of the Act; application of regulations. 381.222 Section 381.222 Animals and Animal Products FOOD... Designating Establishments With Operations Which Would Clearly Endanger the Public Health; Disposition...

  16. 78 FR 63408 - Petition To Amend Animal Welfare Act Regulations To Prohibit Public Contact With Big Cats, Bears...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-24

    ... 5, 2013 (78 FR 47215) is reopened. We will consider all comments that we receive on or before.... SUPPLEMENTARY INFORMATION: On August 5, 2013, we published in the Federal Register (78 FR 47215-47217, Docket No... Animal Welfare Act Regulations To Prohibit Public Contact With Big Cats, Bears, and Nonhuman...

  17. 75 FR 34271 - Federal Acquisition Regulation; FAR Case 2009-010, American Recovery and Reinvestment Act of 2009...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... Federal Register at 74 FR 14636 on March 31, 2009, with a request for comments by June 1, 2009. The... the Federal Register at 74 FR 14636 on March 31, 2009, is adopted as a final rule with the following... Acquisition Regulation; FAR Case 2009-010, American Recovery and Reinvestment Act of 2009 (the Recovery...

  18. CHD1 acts via the Hmgpi pathway to regulate mouse early embryogenesis.

    PubMed

    Suzuki, Shinnosuke; Nozawa, Yusuke; Tsukamoto, Satoshi; Kaneko, Takehito; Manabe, Ichiro; Imai, Hiroshi; Minami, Naojiro

    2015-07-01

    The protein CHD1 is a member of the family of ATPase-dependent chromatin remodeling factors. CHD1, which recognizes trimethylated histone H3 lysine 4, has been implicated in transcriptional activation in organisms ranging from yeast to humans. It is required for pre-mRNA maturation, maintenance of mouse embryonic stem cell pluripotency and rapid growth of the mouse epiblast. However, the function(s) of CHD1 in mouse preimplantation embryos has not yet been examined. Here, we show that loss of CHD1 function led to embryonic lethality after implantation. In mouse embryos in which Chd1 was targeted by siRNA microinjection, the expression of the key regulators of cell fate specification Pou5f1 (also known as Oct4), Nanog and Cdx2 was dramatically decreased, starting at mid-preimplantation gene activation (MGA). Moreover, expression of Hmgpi and Klf5, which regulate Pou5f1, Nanog and Cdx2, was also significantly suppressed at zygotic gene activation (ZGA). Suppression of Hmgpi expression in Chd1-knockdown embryos continued until the blastocyst stage, whereas suppression of Klf5 expression was relieved by the morula stage. Next, we rescued HMGPI expression via Hmgpi mRNA microinjection in Chd1-knockdown embryos. Consequently, Pou5f1, Nanog and Cdx2 expression was restored at MGA and live offspring were recovered. These findings indicate that CHD1 plays important roles in mouse early embryogenesis via activation of Hmgpi at ZGA. PMID:26092847

  19. 47 CFR 54.520 - Children's Internet Protection Act certifications required from recipients of discounts under the...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 3 2012-10-01 2012-10-01 false Children's Internet Protection Act certifications required from recipients of discounts under the federal universal service support mechanism for schools and libraries. 54.520 Section 54.520 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES...

  20. 47 CFR 54.520 - Children's Internet Protection Act certifications required from recipients of discounts under the...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 3 2013-10-01 2013-10-01 false Children's Internet Protection Act certifications required from recipients of discounts under the federal universal service support mechanism for schools and libraries. 54.520 Section 54.520 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES...

  1. 2 CFR 176.50 - Award term-Reporting and registration requirements under section 1512 of the Recovery Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Award term-Reporting and registration requirements under section 1512 of the Recovery Act. 176.50 Section 176.50 Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET GOVERNMENTWIDE GUIDANCE FOR GRANTS AND AGREEMENTS Reserved AWARD TERMS FOR ASSISTANCE AGREEMENTS THAT INCLUDE FUNDS UNDER...

  2. HRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds

    PubMed Central

    Wang, Ran; Liu, Hong; Yang, Huixia; Rodriguez, Pedro L.; Qin, Huanju; Liu, Xin; Wang, Daowen

    2012-01-01

    In this work, we conducted functional analysis of Arabidopsis HRS1 gene in order to provide new insights into the mechanisms governing seed germination. Compared with wild type (WT) control, HRS1 knockout mutant (hrs1-1) exhibited significant germination delays on either normal medium or those supplemented with abscisic acid (ABA) or sodium chloride (NaCl), with the magnitude of the delay being substantially larger on the latter media. The hypersensitivity of hrs1-1 germination to ABA and NaCl required ABI3, ABI4 and ABI5, and was aggravated in the double mutant hrs1-1abi1-2 and triple mutant hrs1-1hab1-1abi1-2, indicating that HRS1 acts as a negative regulator of ABA signaling during seed germination. Consistent with this notion, HRS1 expression was found in the embryo axis, and was regulated both temporally and spatially, during seed germination. Further analysis showed that the delay of hrs1-1 germination under normal conditions was associated with reduction in the elongation of the cells located in the lower hypocotyl (LH) and transition zone (TZ) of embryo axis. Interestingly, the germination rate of hrs1-1 was more severely reduced by the inhibitor of cell elongation, and more significantly decreased by the suppressors of plasmalemma H+-ATPase activity, than that of WT control. The plasmalemma H+-ATPase activity in the germinating seeds of hrs1-1 was substantially lower than that exhibited by WT control, and fusicoccin, an activator of this pump, corrected the transient germination delay of hrs1-1. Together, our data suggest that HRS1 may be needed for suppressing ABA signaling in germinating embryo axis, which promotes the timely germination of Arabidopsis seeds probably by facilitating the proper function of plasmalemma H+-ATPase and the efficient elongation of LH and TZ cells. PMID:22545134

  3. Temporal requirements of the fragile X mental retardation protein in the regulation of synaptic structure.

    PubMed

    Gatto, Cheryl L; Broadie, Kendal

    2008-08-01

    Fragile X syndrome (FraX), caused by the loss-of-function of one gene (FMR1), is the most common inherited form of both mental retardation and autism spectrum disorders. The FMR1 product (FMRP) is an mRNA-binding translation regulator that mediates activity-dependent control of synaptic structure and function. To develop any FraX intervention strategy, it is essential to define when and where FMRP loss causes the manifestation of synaptic defects, and whether the reintroduction of FMRP can restore normal synapse properties. In the Drosophila FraX model, dFMRP loss causes neuromuscular junction (NMJ) synapse over-elaboration (overgrowth, overbranching, excess synaptic boutons), accumulation of development-arrested satellite boutons, and altered neurotransmission. We used the Gene-Switch method to conditionally drive dFMRP expression to define the spatiotemporal requirements in synaptic mechanisms. Constitutive induction of targeted neuronal dFMRP at wild-type levels rescues all synaptic architectural defects in Drosophila Fmr1 (dfmr1)-null mutants, demonstrating a presynaptic requirement for synapse structuring. By contrast, presynaptic dFMRP expression does not ameliorate functional neurotransmission defects, indicating a postsynaptic dFMRP requirement. Strikingly, targeted early induction of dFMRP effects nearly complete rescue of synaptic structure defects, showing a primarily early-development role. In addition, acute dFMRP expression at maturity partially alleviates dfmr1-null defects, although rescue is not as complete as either early or constitutive dFMRP expression, showing a modest capacity for late-stage structural plasticity. We conclude that dFMRP predominantly acts early in synaptogenesis to modulate architecture, but that late dFMRP introduction at maturity can weakly compensate for early absence of dFMRP function. PMID:18579676

  4. Temporal Requirements of the Fragile X Mental Retardation Protein in the Regulation of Synaptic Structure

    PubMed Central

    Gatto, Cheryl L.; Broadie, Kendal

    2009-01-01

    Fragile X Syndrome (FraX), caused by the loss of function of one gene (FMR1), is the most common inherited form of both mental retardation and autism spectrum disorders. The FMR1 product (FMRP) is an mRNA-binding translation regulator that mediates activity-dependent control of synaptic structure and function. To develop any FraX intervention strategy, it is critical to define when and where FMRP loss causes the manifestation of synaptic defects, and whether reintroduction of FMRP can restore normal synapse properties. In the Drosophila FraX model, dFMRP loss causes neuromuscular junction (NMJ) synapse over-elaboration (overgrowth, overbranching, excess synaptic boutons), accumulation of development-arrested satellite boutons, and altered neurotransmission. We have used the Gene-Switch (GS) method to conditionally drive dFMRP to define the spatiotemporal requirements in synaptic mechanisms. Constitutive induction of targeted neuronal dFMRP at wild-type levels rescues all synaptic architectural defects in dfmr1 null mutants, demonstrating a presynaptic requirement for synapse structuring. In contrast, presynaptic dFMRP expression does not ameliorate functional neurotransmission defects, indicating a postsynaptic dFMRP requirement. Strikingly, targeted early induction of dFMRP effects nearly complete rescue of synaptic structure defects, showing a primarily early development role. In addition, acute dFMRP expression at maturity partially alleviates dfmr1 null defects, although rescue is not as complete as either early or constitutive dFMRP expression, showing a modest capacity for late-stage structural plasticity. We conclude that dFMRP predominantly acts early in synaptogenesis to modulate architecture, but that late dFMRP introduction at maturity can weakly compensate for early absence of dFMRP function. PMID:18579676

  5. Deeming Tobacco Products To Be Subject to the Federal Food, Drug, and Cosmetic Act, as Amended by the Family Smoking Prevention and Tobacco Control Act; Restrictions on the Sale and Distribution of Tobacco Products and Required Warning Statements for Tobacco Products. Final rule.

    PubMed

    2016-05-10

    The Food and Drug Administration (FDA) is issuing this final rule to deem products meeting the statutory definition of "tobacco product,'' except accessories of the newly deemed tobacco products, to be subject to the Federal Food, Drug, and Cosmetic Act (the FD&C Act), as amended by the Family Smoking Prevention and Tobacco Control Act (Tobacco Control Act). The Tobacco Control Act provides FDA authority to regulate cigarettes, cigarette tobacco, roll-your-own tobacco, smokeless tobacco, and any other tobacco products that the Agency by regulation deems to be subject to the law. With this final rule, FDA is extending the Agency's "tobacco product'' authorities in the FD&C Act to all other categories of products that meet the statutory definition of "tobacco product" in the FD&C Act, except accessories of such newly deemed tobacco products. This final rule also prohibits the sale of "covered tobacco products" to individuals under the age of 18 and requires the display of health warnings on cigarette tobacco, roll-your own tobacco, and covered tobacco product packages and in advertisements. FDA is taking this action to reduce the death and disease from tobacco products. In accordance with the Tobacco Control Act, we consider and intend the extension of our authorities over tobacco products and the various requirements and prohibitions established by this rule to be severable. PMID:27192730

  6. p50cdc37 Acting in Concert with Hsp90 Is Required for Raf-1 Function†

    PubMed Central

    Grammatikakis, Nicholas; Lin, Jun-Hsiang; Grammatikakis, Aliki; Tsichlis, Philip N.; Cochran, Brent H.

    1999-01-01

    Genetic screens in Drosophila have identified p50cdc37 to be an essential component of the sevenless receptor/mitogen-activated kinase protein (MAPK) signaling pathway, but neither the function nor the target of p50cdc37 in this pathway has been defined. In this study, we examined the role of p50cdc37 and its Hsp90 chaperone partner in Raf/Mek/MAPK signaling biochemically. We found that coexpression of wild-type p50cdc37 with Raf-1 resulted in robust and dose-dependent activation of Raf-1 in Sf9 cells. In addition, p50cdc37 greatly potentiated v-Src-mediated Raf-1 activation. Moreover, we found that p50cdc37 is the primary determinant of Hsp90 recruitment to Raf-1. Overexpression of a p50cdc37 mutant which is unable to recruit Hsp90 into the Raf-1 complex inhibited Raf-1 and MAPK activation by growth factors. Similarly, pretreatment with geldanamycin (GA), an Hsp90-specific inhibitor, prevented both the association of Raf-1 with the p50cdc37-Hsp90 heterodimer and Raf-1 kinase activation by serum. Activation of Raf-1 via baculovirus coexpression with oncogenic Src or Ras in Sf9 cells was also strongly inhibited by dominant negative p50cdc37 or by GA. Thus, formation of a ternary Raf-1–p50cdc37–Hsp90 complex is crucial for Raf-1 activity and MAPK pathway signaling. These results provide the first biochemical evidence for the requirement of the p50cdc37-Hsp90 complex in protein kinase regulation and for Raf-1 function in particular. PMID:10022854

  7. Transferrin synthesis by small cell lung cancer cells acts as an autocrine regulator of cellular proliferation.

    PubMed Central

    Vostrejs, M; Moran, P L; Seligman, P A

    1988-01-01

    Since transferrin is required for cellular proliferation, we investigated transferrin synthesis by a small cell lung cancer line (NCI-H510) that survives in serum-free media without added transferrin. Immunoassays for human transferrin demonstrated that these cells contained immunoreactive human transferrin. Immunofluorescence studies showed that the protein is expressed on the surface of cells, presumably bound to transferrin receptor. Media conditioned by NCI-H510 cells support proliferation of human leukemic cells that would not survive in media lacking transferrin. [35S]Methionine incorporation documented transferrin synthesis by NCI-H510 cells as well as three other small cell lines. Transferrin synthesis by NCI-H510 cells increased more than 10-fold when cells entered active phases of the cell cycle, and this increase was seen before large increases in transferrin-receptor expression. Further experiments examining the effects of agents that affect iron metabolism show that the addition of transferrin-iron or hemin to the media is associated with a more rapid initial rate of proliferation and lower rates of transferrin synthesis than control cells. Gallium salts, which inhibit iron uptake, inhibited proliferation of these cells. If the cells recovered from this effect, transferrin synthesis remained greatly increased compared to control. We conclude that transferrin synthesis by these malignant cells is ultimately related to an iron requirement for cellular proliferation. It appears that this synthesized transferrin acts as part of an important autocrine mechanism permitting proliferation of these cells, and perhaps permitting tumor cell growth in vivo in areas not well vascularized. Images PMID:2839550

  8. 75 FR 69423 - Information Collection Requirements; Defense Federal Acquisition Regulation Supplement; Small...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-12

    ... Control Number 0704-0386. Needs and Uses: DoD uses this information in assessing contractor compliance... Regulations System, Department of Defense (DoD). ACTION: Notice and request for comments regarding a proposed...)(A) of the Paperwork Reduction Act of 1995 (44 U.S.C. chapter 35), DoD announces the...

  9. 77 FR 74177 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Production...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ... Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice; request for comments regarding a... 3506(c)(2)(A) of the Paperwork Reduction Act of 1995 (44 U.S.C. Chapter 35), DoD announces the proposed.... DoD invites comments on: (a) Whether the proposed collection of information is necessary for...

  10. 78 FR 69267 - Defense Federal Acquisition Regulation Supplement: Requirements Relating to Supply Chain Risk...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-18

    ...DoD is issuing an interim rule amending the Defense Federal Acquisition Regulation Supplement (DFARS) to implement a section of the National Defense Authorization Act (NDAA) for Fiscal Year (FY) 2011, as amended by the NDAA for FY 2013. This interim rule allows DoD to consider the impact of supply chain risk in specified types of procurements related to national security...

  11. 76 FR 10527 - Regulatory Flexibility Act: Section 610 Review of National Organic Program Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-25

    ... regulations implementing the National Organic Program (NOP) were published December 21, 2000 (65 FR 80548... FR 14827), its schedule to review certain regulations, including the NOP regulations, under criteria... Organic Program Regulations AGENCY: Agricultural Marketing Service, USDA. ACTION: Review and request...

  12. 49 CFR 27.19 - Compliance with Americans with Disabilities Act requirements and FTA policy.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) Recipients subject to this part (whether public or private entities as defined in 49 CFR part 37) shall.... 12101-12213) including the Department's ADA regulations (49 CFR parts 37 and 38), the regulations of the Department of Justice implementing titles II and III of the ADA (28 CFR parts 35 and 36), and the...

  13. 49 CFR 27.19 - Compliance with Americans with Disabilities Act requirements and FTA policy.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) Recipients subject to this part (whether public or private entities as defined in 49 CFR part 37) shall.... 12101-12213) including the Department's ADA regulations (49 CFR parts 37 and 38), the regulations of the Department of Justice implementing titles II and III of the ADA (28 CFR parts 35 and 36), and the...

  14. 49 CFR 27.19 - Compliance with Americans with Disabilities Act requirements and FTA policy.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) Recipients subject to this part (whether public or private entities as defined in 49 CFR part 37) shall.... 12101-12213) including the Department's ADA regulations (49 CFR parts 37 and 38), the regulations of the Department of Justice implementing titles II and III of the ADA (28 CFR parts 35 and 36), and the...

  15. 75 FR 60263 - Federal Acquisition Regulation; Offering a Construction Requirement-8(a) Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ... Federal Acquisition Regulation; Offering a Construction Requirement--8(a) Program AGENCIES: Department of... submitting offering letters to SBA for a construction requirement for which a specific offeror is nominated... offerings for construction ] requirements be submitted to the SBA District Office for the geographical...

  16. 78 FR 25445 - Federal Acquisition Regulation; Submission for OMB Review; Quality Assurance Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... information collection requirement concerning quality assurance requirements. A notice was published in the Federal Register at 77 FR 67366, on November 11, 2012. No comments were received. DATES: Submit comments... Regulation; Submission for OMB Review; Quality Assurance Requirements AGENCY: Department of Defense...

  17. 77 FR 62495 - Federal Acquisition Regulation; Submission for OMB Review; Novation/Change of Name Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-15

    ... approved information collection requirement concerning Novation/Change of Name Requirements. A notice was published in the Federal Register at 77 FR 29983, on May 21, 2012. One respondent submitted comments. Public... Regulation; Submission for OMB Review; Novation/Change of Name Requirements AGENCY: Department of...

  18. 16 CFR 312.3 - Regulation of unfair or deceptive acts or practices in connection with the collection, use, and...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... children on the Internet. 312.3 Section 312.3 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS... information from and about children on the Internet. General requirements. It shall be unlawful for...

  19. 16 CFR 312.3 - Regulation of unfair or deceptive acts or practices in connection with the collection, use, and...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... children on the Internet. 312.3 Section 312.3 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS... information from and about children on the Internet. General requirements. It shall be unlawful for...

  20. 16 CFR 312.3 - Regulation of unfair or deceptive acts or practices in connection with the collection, use, and...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... children on the Internet. 312.3 Section 312.3 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS... information from and about children on the Internet. General requirements. It shall be unlawful for...

  1. 16 CFR 312.3 - Regulation of unfair or deceptive acts or practices in connection with the collection, use, and...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... children on the Internet. 312.3 Section 312.3 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS... information from and about children on the Internet. General requirements. It shall be unlawful for...

  2. 16 CFR 312.3 - Regulation of unfair or deceptive acts or practices in connection with the collection, use, and...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... children on the Internet. 312.3 Section 312.3 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS... information from and about children on the Internet. General requirements. It shall be unlawful for...

  3. 76 FR 41434 - Removal of Certain Requirements Related to the Prescription Drug Marketing Act; Opportunity for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-14

    .... drug supply chain. Among other things, the PDMA, in section 503(e)(1)(A) of the FD&C Act (21 U.S.C. 353... or control number(s) in the pedigree as well. FDA continues to believe that drug supply chain... our drug supply chain and the action proposed in this document should not be interpreted to mean...

  4. Training Child Welfare Workers To Meet the Requirements of the Indian Child Welfare Act.

    ERIC Educational Resources Information Center

    Bending, Raymond L.

    1997-01-01

    Describes a culturally sensitive program developed by the University of Washington School of Social Work, the Washington Department of Social and Health Services, and 26 Indian tribes in Washington State that trained 34 child welfare personnel to better implement the intent of the Indian Child Welfare Act of 1978. Contains program evaluation…

  5. 78 FR 65557 - Commission Information Collection Requirements Under the Paperwork Reduction Act; OMB Control...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-01

    ... is codifying the control numbers that have been issued by the Office of Management and Budget (OMB... numbers assigned by the Office of Management and Budget (OMB) pursuant to the Paperwork Reduction Act of... CFR 1022.43. 0145 640; 12 CFR 1022.70. 0150 318. 0156 12 CFR part 1014. 0157 12 CFR part 1015. ]...

  6. 78 FR 14309 - Implementation of the FDA Food Safety Modernization Act Provision Requiring FDA To Establish...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-05

    ...In September 2011, the Food and Drug Administration (FDA or the Agency) asked the Institute of Food Technologists (IFT) to execute product tracing pilot projects as described in the FDA Food Safety Modernization Act (FSMA). FDA recently released a report from IFT on these pilot projects, entitled ``Pilot Projects for Improving Product Tracing along the Food Supply System.'' FDA is announcing......

  7. 77 FR 28790 - Medical Loss Ratio Requirements Under the Patient Protection and Affordable Care Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ... Patient Protection and Affordable Care Act (75 FR 34538) published on June 17, 2010, it was estimated that... on May 5, 2010 (75 FR 24470) relating to the Federal health care reform insurance Web Portal... Federal Register on April 14, 2010 (75 FR 19297). On December 1, 2010, the Department of Health and...

  8. 76 FR 78130 - Mortgage Acts and Practices-Advertising (Regulation N); Mortgage Assistance Relief Services...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-16

    ... Advance Notice of Proposed Rulemaking: Mortgage Acts and Practices, 74 FR 26118 (June 1, 2009), that.... See 76 FR 43569 (July 21, 2011). The Omnibus Appropriations Act, as amended, authorizes the Bureau to... response to a notice published at 76 FR 75825 (Dec. 5, 2011) concerning its efforts to identify...

  9. 75 FR 12377 - Native American Graves Protection and Repatriation Act Regulations-Disposition of Culturally...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... first rules to implement the Act on December 4, 1995 (43 CFR part 10, 60 FR 62158). --We published rules for assessing civil penalties under the Act on April 3, 2003 (43 CFR 10.12, 68 FR 16354). --We....13, 72 FR 13189). --We are publishing this rule today. --We are developing additional rules to...

  10. 78 FR 41835 - Inflation Adjustments to the Price-Anderson Act Financial Protection Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-12

    ... consistent with the Plain Writing Act as well as the Presidential Memorandum, ``Plain Language in Government... made the initial changes to the Price-Anderson Act amounts on October 27, 2005 (70 FR 61885), and the first periodic inflation adjustments on September 29, 2008 (73 FR 56451). This final rule makes...

  11. Photogrammetry status in the act geodetic and cartographic law and connected regulations. (Polish Title: Status fotogrametrii w ustawie prawo geodezyjne i kartograficzne i przepisach powiazanych)

    NASA Astrophysics Data System (ADS)

    Pyka, K.; Myszka, P.

    2015-12-01

    The topic of the article is the analysis of regulations referring to photogrammetry, which have been in effect since the latest amendments in the Act Geodetic and Cartographic Law were introduced. Among many new rules, the four most important for regulations for photogrammetry were selected for the analysis. The largest part of analyses deals with so-called technical standards. This regulation introduces the term: geodetic photogrammetric measurement and places identical accuracy requirements as for field measurement. However, photogrammetric measurement, in this law is treated less thoroughly than other techniques. Often in this interpretation of the regulations there are different opinions between the ones who order the measurements and those who carry them out. The article shows which regulations are not satisfactorily clear and can be interpreted in different ways. Moreover, the article refers to unsatisfactory consistency between the analysed enactments. Finally, it was stated that the regulations, despite the indicated drawbacks, allow the application of photogrammetry in surveying and there is a prospect of granting photogrammetry the rank of the operation method, especially in large projects, e.g. the update of cadastral maps and land survey maps.

  12. 29 CFR 4.117 - Work subject to requirements of Walsh-Healey Act.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... required to be completely or substantially torn down into individual components parts; and (ii... following conditions exist: (i) The item or equipment is required to be completely or substantially...

  13. 29 CFR 4.117 - Work subject to requirements of Walsh-Healey Act.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... required to be completely or substantially torn down into individual components parts; and (ii... following conditions exist: (i) The item or equipment is required to be completely or substantially...

  14. 29 CFR 4.117 - Work subject to requirements of Walsh-Healey Act.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... required to be completely or substantially torn down into individual components parts; and (ii... following conditions exist: (i) The item or equipment is required to be completely or substantially...

  15. 29 CFR 4.117 - Work subject to requirements of Walsh-Healey Act.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... required to be completely or substantially torn down into individual components parts; and (ii... following conditions exist: (i) The item or equipment is required to be completely or substantially...

  16. 29 CFR 4.117 - Work subject to requirements of Walsh-Healey Act.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... required to be completely or substantially torn down into individual components parts; and (ii... following conditions exist: (i) The item or equipment is required to be completely or substantially...

  17. National health information privacy: regulations under the Health Insurance Portability and Accountability Act.

    PubMed

    Gostin, L O

    2001-06-20

    Health information privacy is important in US society, but existing federal and state law does not offer adequate protection. The Department of Health and Human Services, under powers granted by the Health Insurance Portability and Accountability Act of 1996, recently issued a final rule providing systematic, nationwide health information privacy protection. The rule is extensive in its scope, applying to health plans, health care clearinghouses, and health care providers (hospitals, clinics, and health departments) who conduct financial transactions electronically ("covered entities"). The rule applies to personally identifiable information in any form, whether communicated electronically, on paper, or orally. The rule does not preempt state law that affords more stringent privacy protection; thus, the health care industry will have to comply with multiple layers of federal and state law. The rule affords patients rights to education about privacy safeguards, access to their medical records, and a process for correction of records. It also requires the patient's permission for disclosures of personal information. While privacy is an important value, it may conflict with public responsibilities to use data for social goods. The rule has special provisions for disclosure of health information for research, public health, law enforcement, and commercial marketing. The privacy debate will continue in Congress and within the president's administration. The primary focus will be on the costs and burdens on health care providers, the ability of health care professionals to use and share full medical information when treating patients, the provision of patient care in a timely and efficient manner, and parents' access to information about the health of their children. PMID:11410101

  18. Powerplant and Industrial Fuel Use Act. Annual report. [Annual report required by Section 108, Public Law 95-620

    SciTech Connect

    Not Available

    1983-03-01

    This annual report has been prepared for Congress by the Department of Energy in conjunction with the Environmental Protection Agency, as required by section 806 of the Powerplant and Industrial Fuel Use Act of 1978 (FUA) as amended, Public Law 95-620, enacted November 9, 1978. This annual report describes actions taken, under the provisions of the legislation, to encourage the use of indigenous energy resources in electric powerplants and major fuel-burning installations in the electric utility, industrial, and Federal governmental sectors. This report addresses implementation activities during calendar year 1982 under FUA and section 2 of the Energy Supply and Environmental Coordination Act of 1974, Public Law 93-319, and assesses the effectiveness of both laws in achieving their purposes. The report also includes summary information on natural gas consumption by electric utilities in 1977 and 1982, as required by section 711(c) of FUA, as amended by section 1021 of the Omnibus Budget Reconciliation Act of 1981, Public Law 97-35. Copies of this report were submitted to the Committee on Energy and Natural Resources of the US Senate and to the Committee on Energy and Commerce of the US House of Representatives, as required by section 807 of FUA. A companion report, as required by section 403(c) of FUA, will be submitted to Congress covering implementation of FUA at fuel-burning facilities owned or operated by the Federal Government.

  19. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2013-04-01 2013-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  20. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2010-04-01 2010-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  1. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2014-04-01 2014-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  2. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2011-04-01 2011-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  3. GP Supervisors' Experience in Supporting Self-Regulated Learning: A Balancing Act

    ERIC Educational Resources Information Center

    Sagasser, Margaretha H.; Kramer, Anneke W. M.; van Weel, Chris; van der Vleuten, Cees P. M.

    2015-01-01

    Self-regulated learning is essential for professional development and lifelong learning. As self-regulated learning has many inaccuracies, the need to support self-regulated learning has been recommended. Supervisors can provide such support. In a prior study trainees reported on the variation in received supervisor support. This study aims at…

  4. Regulation of DNA replication in irradiated cells by trans-acting factors

    SciTech Connect

    Wang, Y.; Huq, M.S.; Cheng, X.; Iliakis, G.

    1995-05-01

    We compared DNA replication activity in cytoplasmic extracts prepared from irradiated and nonirradiated HeLa cells using a simian virus 40 (SV40)-based in vitro replication assay. The assay measures semi-conservative DNA replication in a plasmid carrying the SV40 origin of replication and requires SV40 T antigen as the sole noncellular protein. The plasmid DNA used in the replication reaction is never exposed to radiation. We find that replication of plasmid DNA is significantly reduced when cytoplasmic extracts from irradiated cells are used. Since plasmid replication proceeds to completion in extracts from irradiated cells, the observed reduction in the overall replication activity is probably due to a reduction in the efficiency of initiation events. The degree of inhibition of DNA replication after exposure to 10, 30 and 50 Gy X rays as measured in vitro using this assay is similar to that measured in intact cells immediately before processing for extract preparation. These observations are compatible with the induction or activation by ionizing radiation of a factor(s) that inhibits in trans DNA replication. The results contribute to our understanding of the mechanism(s) developed by the cells to regulate DNA replication when exposed to clastogenic agents. Such processes may be of significance in the restoration of DNA integrity, and may define yet another checkpoint operating during S at the level of clusters of replicons. 26 refs., 4 figs.

  5. 16 CFR 301.3 - English language requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false English language requirements. 301.3 Section 301.3 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.3 English language requirements. All information required under the act and...

  6. 12 CFR 749.5 - Format for records required by other NCUA regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Format for records required by other NCUA regulations. 749.5 Section 749.5 Banks and Banking NATIONAL CREDIT UNION ADMINISTRATION REGULATIONS AFFECTING CREDIT UNIONS RECORDS PRESERVATION PROGRAM AND APPENDICES-RECORD RETENTION GUIDELINES; CATASTROPHIC...

  7. 27 CFR 70.411 - Imposition of taxes, qualification requirements, and regulations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... operation of distilled spirits plants. Part 19 of title 27 CFR contains the regulations relating to the...) Miscellaneous liquor transactions. Part 29 of 27 CFR contains miscellaneous regulations relative to the... requirements therefor. (3) (4) Gauging of distilled spirits. Part 30 of title 27 CFR contains the...

  8. 27 CFR 70.411 - Imposition of taxes, qualification requirements, and regulations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... operation of distilled spirits plants. Part 19 of title 27 CFR contains the regulations relating to the...) Miscellaneous liquor transactions. Part 29 of 27 CFR contains miscellaneous regulations relative to the... requirements therefor. (3) (4) Gauging of distilled spirits. Part 30 of title 27 CFR contains the...

  9. 27 CFR 70.411 - Imposition of taxes, qualification requirements, and regulations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... operation of distilled spirits plants. Part 19 of title 27 CFR contains the regulations relating to the...) Miscellaneous liquor transactions. Part 29 of 27 CFR contains miscellaneous regulations relative to the... requirements therefor. (3) (4) Gauging of distilled spirits. Part 30 of title 27 CFR contains the...

  10. 27 CFR 70.411 - Imposition of taxes, qualification requirements, and regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... operation of distilled spirits plants. Part 19 of title 27 CFR contains the regulations relating to the...) Miscellaneous liquor transactions. Part 29 of 27 CFR contains miscellaneous regulations relative to the... requirements therefor. (3) (4) Gauging of distilled spirits. Part 30 of title 27 CFR contains the...

  11. 78 FR 72620 - Federal Acquisition Regulation; Higher-Level Contract Quality Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-03

    ... comment in the Federal Register at 78 FR 28780 on May 16, 2013. 2. FAR Case 2013-002, entitled ``Expanded... RIN 9000-AM65 Federal Acquisition Regulation; Higher-Level Contract Quality Requirements AGENCY... Acquisition Regulation (FAR) to clarify when to use higher-level quality standards in solicitations...

  12. 78 FR 32547 - Loan Originator Compensation Requirements Under the Truth in Lending Act (Regulation Z...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ..., 2013, at 78 FR 11280, is effective January 10, 2014, with the exception of the amendments to 12 CFR...\\ Public Law 111-203, 124 Stat. 1376 (2010). \\2\\ 78 FR 11279 (Feb. 15, 2013). A. Title XIV Rulemaking... whether the provision should become effective sooner than January 2014.\\7\\ \\6\\ 77 FR 55272 (Sept. 7,...

  13. 78 FR 27308 - Loan Originator Compensation Requirements Under the Truth In Lending Act (Regulation Z...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-10

    ... adopting Sec. 1026.36(i). \\1\\ 78 FR 11279 (Feb. 15, 2013). A. Title XIV Rulemaking Effective Dates In... become effective sooner than January 2014.\\5\\ \\5\\ 77 FR 55272 (Sept. 7, 2012). The Bureau received very... OF CONSUMER FINANCIAL PROTECTION 12 CFR Part 1026 RIN 3170-AA37 Loan Originator...

  14. The Regulation of Medical Computer Software as a “Device” under the Food, Drug, and Cosmetic Act

    PubMed Central

    Brannigan, Vincent

    1986-01-01

    Recent developments in computer software have raised the possibility that federal regulators may claim to control medical computer software as a “device” under the Food, Drug and Cosmetic Act. The purpose of this paper is to analyze the FDCA to determine whether computer software is included in the statutory scheme, examine constitutional arguments relating to computer software, and discuss regulatory principles that should be taken into account when deciding appropriate regulation. This paper is limited to computer program output used by humans in deciding appropriate medical therapy for a patient.

  15. 78 FR 20291 - Fisheries of the United States; Billfish Conservation Act of 2012 Implementing Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-04

    ...NMFS issues this advance notice of proposed rulemaking (ANPR) to provide background information and request public comment on potential issues related to the implementation of the Billfish Conservation Act of...

  16. Employee Retirement Income Security Act of 1974; rules and regulations for administration and enforcement; claims procedure--Department of Labor. Notice of proposed rulemaking.

    PubMed

    1998-09-01

    This document contains a proposed regulation revising the minimum requirements for benefit claims procedures of employee benefit plans covered by Title I of the Employee Retirement Income Security Act of 1974 (ERISA or the Act). This proposed regulation would establish new standards for the processing of group health disability, pension, and other employee benefit plan claims filed by participants and beneficiaries. In the case of group health plans, as well as certain plans providing disability benefits, the new standards are intended to ensure more timely benefit determinations, improved access to information on which a benefit determination is made, and greater assurance that participants and beneficiaries will be afforded a full and fair review of denied claims. If adopted as final, the proposed regulation would affect participants and beneficiaries of employee benefit plans, plan, fiduciaries, and others who assist in the provision of plan benefits, such as third-party benefits administrators and health service providers or health maintenance organizations that provide benefits to participants and beneficiaries of employee benefit plans. PMID:10185189

  17. Colleges and Universities That Do Not Require SAT® or ACT Scores. Research Notes. RN-18

    ERIC Educational Resources Information Center

    Milewski, Glenn B.; Camara, Wayne J.

    2002-01-01

    In recent years, conflicting reports have circulated about the number of colleges and universities that have made standardized tests optional, rather than required, for admission. The National Center for Fair and Open Testing, or FairTest, has widely publicized a list of 391 colleges and universities that, it claims, do not require admissions…

  18. 78 FR 6056 - Designation of Payor as Agent To Perform Acts Required of an Employer

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ... Federal Register on January 13, 2010, (75 FR 1735-01). Pursuant to section 3504 and the regulations, the... common law analysis. In unique circumstances, an individual may be an employee of more than one employer... must file Forms W-2, Wage and Tax Statement, and a Form W-3, Transmittal of Wage and Tax...

  19. Regulation of caulimovirus gene expression and the involvement of cis-acting elements on both viral transcripts.

    PubMed

    Scholthof, H B; Wu, F C; Gowda, S; Shepherd, R J

    1992-09-01

    In a further analysis of gene regulation of figwort mosaic virus (FMV), a caulimovirus, we studied transient gene expression with modified viral genomes in Nicotiana edwardsonii cell suspension protoplasts. The results demonstrated that the presence of the promoter for the full-length RNA interferes with expression from the separate downstream promoter for gene VI. In addition, expression of gene VI was inhibited by cis-acting sequences within gene VI itself. Both inhibitory effects could be partially relieved by coelectroporation with a plasmid that produces gene VI protein, demonstrating that expression of gene VI is transactivated by its own product. Subsequent expression studies with partially redundant FMV plasmids containing a reporter gene in frame with gene IV showed that efficient transactivation of CAT expression relies on a cis-acting element inside the downstream gene VI. Insertions of a transcriptional terminator upstream of the cis-acting element for premature termination of transcription showed that the cis-acting region is not a DNA element but is active only as a feature of the RNA transcript. We conclude that the cis-acting element, together with the transacting gene VI product, enhances expression of all major genes, including gene VI, from the polycistronic mRNA and the separate mRNA for gene VI. PMID:1529539

  20. 12 CFR 208.36 - Reporting requirements for State member banks subject to the Securities Exchange Act of 1934.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... data prepared in accordance with GAAP and disclose any material contingencies, as required by Article 10 of the Commission's Regulation S-X (17 CFR 210.10-01), in the Management's Discussion and Analysis...'s rules shall be paid to the Board. (3) Public inspection. Copies of the registration...

  1. 12 CFR 208.36 - Reporting requirements for State member banks subject to the Securities Exchange Act of 1934.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... data prepared in accordance with GAAP and disclose any material contingencies, as required by Article 10 of the Commission's Regulation S-X (17 CFR 210.10-01), in the Management's Discussion and Analysis...'s rules shall be paid to the Board. (3) Public inspection. Copies of the registration...

  2. 12 CFR 208.36 - Reporting requirements for State member banks subject to the Securities Exchange Act of 1934.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... data prepared in accordance with GAAP and disclose any material contingencies, as required by Article 10 of the Commission's Regulation S-X (17 CFR 210.10-01), in the Management's Discussion and Analysis...'s rules shall be paid to the Board. (3) Public inspection. Copies of the registration...

  3. 15 CFR Supplement No. 1 to Part 730 - Information Collection Requirements Under the Paperwork Reduction Act: OMB Control Numbers

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND... requirements for the Bureau of Industry and Security by the Office of Management and Budget (OMB), pursuant to... Supply Regulations—Petroleum Products § 754.3. 0694-0047 Technology Letter of Explanation Supplement...

  4. 15 CFR Supplement No. 1 to Part 730 - Information Collection Requirements Under the Paperwork Reduction Act: OMB Control Numbers

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND... requirements for the Bureau of Industry and Security by the Office of Management and Budget (OMB), pursuant to... Supply Regulations—Petroleum Products § 754.3. 0694-0047 Technology Letter of Explanation Supplement...

  5. 15 CFR Supplement No. 1 to Part 730 - Information Collection Requirements Under the Paperwork Reduction Act: OMB Control Numbers

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND... requirements for the Bureau of Industry and Security by the Office of Management and Budget (OMB), pursuant to... Supply Regulations—Petroleum Products § 754.3. 0694-0047 Technology Letter of Explanation Supplement...

  6. Coverage of Prandial Insulin Requirements by Means of an Ultra-Rapid-Acting Inhaled Insulin

    PubMed Central

    Boss, Anders H.; Petrucci, Richard; Lorber, Daniel

    2012-01-01

    Barriers to the use of prandial insulin regimens include inadequate synchronization of insulin action to postprandial plasma glucose excursions as well as a significant risk of hypoglycemia and weight gain. Technosphere® insulin (TI) is an inhaled ultra-rapid-acting human insulin that is quickly absorbed in the alveoli. With a time to maximum plasma drug concentration of approximately 14 min and a time to maximum effect of 35 to 40 min, TI more closely matches the postprandial insulin concentrations seen in nondiabetic individuals. Studies have shown that long-term administration of prandial TI in combination with long-acting basal insulin results in reductions in hemoglobin A1c comparable to conventional subcutaneously injected prandial insulins but with improved control of early postprandial BG. Furthermore, TI has been associated with less weight gain and a lower incidence of hypoglycemia, which may enhance patient satisfaction and acceptability of insulin therapy. This review discusses the clinical properties of TI and proposes strategies for optimal use. PMID:22920801

  7. 77 FR 16196 - Delaying the Compliance Date for Certain Requirements of the Regulations Implementing Titles II...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ...By this rule, the Department of Justice is proposing to extend the date for compliance with certain requirements in the 2010 Americans with Disabilities Act (ADA) Standards for Accessible Design that relate to provision of accessible entry and exit for existing swimming pools and spas. Concurrently with the publication of this Notice of Proposed Rulemaking (NPRM), the Department is publishing......

  8. 75 FR 61653 - Advance Notice of Proposed Rulemaking Regarding Authority To Require Supervision and Regulation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ...Section 113 of the Dodd-Frank Wall Street Reform and Consumer Protection Act (the ``DFA'') gives the Financial Stability Oversight Council (the ``Council'') the authority to require that a nonbank financial company be supervised by the Board of Governors of the Federal Reserve System (``Board of Governors'') and subject to prudential standards if the Council determines that material financial......

  9. 48 CFR 52.204-11 - American Recovery and Reinvestment Act-Reporting Requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Text of Provisions and Clauses 52.204-11 American Recovery and Reinvestment Act—Reporting Requirements... North American Industry Classification System (NAICS) code. (vi) Funding agency. (vii) A description...

  10. 48 CFR 52.204-11 - American Recovery and Reinvestment Act-Reporting Requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Text of Provisions and Clauses 52.204-11 American Recovery and Reinvestment Act—Reporting Requirements... North American Industry Classification System (NAICS) code. (vi) Funding agency. (vii) A description...

  11. 12 CFR 350.12 - Disclosure required by applicable banking or securities law or regulations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 4 2011-01-01 2011-01-01 false Disclosure required by applicable banking or securities law or regulations. 350.12 Section 350.12 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION... STATE NONMEMBER BANKS § 350.12 Disclosure required by applicable banking or securities law...

  12. 75 FR 14448 - Food and Drug Administration Clinical Trial Requirements, Regulations, Compliance, and Good...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-25

    ... HUMAN SERVICES Food and Drug Administration Food and Drug Administration Clinical Trial Requirements... announcing a public workshop entitled ``FDA Clinical Trial Requirements, Regulations, Compliance, and Good... representatives. The program will focus on the relationships among the FDA and clinical trial staff,...

  13. 77 FR 59139 - Prompt Corrective Action, Requirements for Insurance, and Promulgation of NCUA Rules and Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    ... amended by IRPS 03-2, and two NCUA regulations that apply asset thresholds to grant relief from risk-based...'' credit union for determining whether risk-based net worth requirements apply, and 12 CFR 741.3(b)(5)(i... interest rate risk rule requirements. \\1\\ IRPS 03-2, 68 FR 31949 (May 29, 2003). B. Why is the...

  14. 12 CFR 350.12 - Disclosure required by applicable banking or securities law or regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Disclosure required by applicable banking or securities law or regulations. 350.12 Section 350.12 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION... STATE NONMEMBER BANKS § 350.12 Disclosure required by applicable banking or securities law...

  15. 49 CFR 390.11 - Motor carrier to require observance of driver regulations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 5 2011-10-01 2011-10-01 false Motor carrier to require observance of driver... Motor carrier to require observance of driver regulations. Whenever in part 325 of subchapter A or in this subchapter a duty is prescribed for a driver or a prohibition is imposed upon the driver, it...

  16. 49 CFR 390.11 - Motor carrier to require observance of driver regulations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 5 2012-10-01 2012-10-01 false Motor carrier to require observance of driver... Motor carrier to require observance of driver regulations. Whenever in part 325 of subchapter A or in this subchapter a duty is prescribed for a driver or a prohibition is imposed upon the driver, it...

  17. 49 CFR 390.11 - Motor carrier to require observance of driver regulations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 5 2013-10-01 2013-10-01 false Motor carrier to require observance of driver... Motor carrier to require observance of driver regulations. Whenever in part 325 of subchapter A or in this subchapter a duty is prescribed for a driver or a prohibition is imposed upon the driver, it...

  18. 49 CFR 390.11 - Motor carrier to require observance of driver regulations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 5 2014-10-01 2014-10-01 false Motor carrier to require observance of driver... Motor carrier to require observance of driver regulations. Whenever in part 325 of subchapter A or in this subchapter a duty is prescribed for a driver or a prohibition is imposed upon the driver, it...

  19. 76 FR 10234 - Amendment to the Bank Secrecy Act Regulations-Reports of Foreign Financial Accounts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... Regulations (CFR) for BSA regulations. (See 75 FR 65806 (October 26, 2010) (Transfer and Reorganization of... draft of which appeared in the Federal Register as an attachment to the NPRM. \\3\\ See 75 FR 8844... foreign financial institution.\\5\\ \\4\\ 75 FR 8851 (February 26, 2010) (Emphasis added). \\5\\ A revised...

  20. 75 FR 51420 - Removing Regulations Implementing the Fish and Wildlife Conservation Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-20

    ... implementing regulations (50 CFR 83) in the May 6, 2010, Federal Register (75 FR 24862) and invited public... with Native American Tribal Governments'' (59 FR 22951), E.O. 13175, and 512 DM 2. We have determined... Fish and Wildlife Service 50 CFR Part 83 RIN 1018-AX00 Removing Regulations Implementing the Fish...