Science.gov

Sample records for act request dna

  1. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  2. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  3. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  4. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  5. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  6. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR 16... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Privacy Act requests by inmates....

  7. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false FOIA and Privacy Act requests. 0.506 Section 0... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for....461), of the Privacy Act of 1974, (See § 0.554) shall be processed in accordance with the...

  8. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.6 Procedures for Privacy Act requests. (a) Any individual may— (1)...

  9. 78 FR 62629 - Proposed Information Collection Request; Comment Request; Clean Water Act Section 404 State...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ... AGENCY Proposed Information Collection Request; Comment Request; Clean Water Act Section 404 State... Environmental Protection Agency is planning to submit an information collection request (ICR), ``Clean Water Act... information collection as described below. This is a proposed extension of the ICR, which is...

  10. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) A Appendix A to Part 2507 Public Welfare Regulations Relating to Public Welfare (Continued... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter (Sample... Information Act Request. Dear____: This is a request under the Freedom of Information Act. I request that...

  11. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Freedom of Information Act Request Letter (Sample... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter (Sample) Freedom of Information Act Officer Name of Agency Address of Agency City, State, Zip Code Re: Freedom...

  12. 76 FR 32991 - Request (ICR) for the Family Medical Leave Act (FMLA) Employee and Employer Surveys; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-07

    ... the Family and Medical Leave Act of 1993. Type of Review: New Information Collection Request. Agency... with family and medical leave under the Family and Medical Leave Act (FMLA). A copy of the proposed... Hour Division Request (ICR) for the Family Medical Leave......

  13. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Requirements for making Privacy Act requests. 1830.2 Section 1830.2 Administrative Personnel OFFICE OF SPECIAL COUNSEL PRIVACY § 1830.2 Requirements for making Privacy Act requests. (a) How made and addressed. A request for OSC records under...

  14. 78 FR 32227 - Perishable Agricultural Commodities Act; Request for Extension and Revision of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-29

    ... Agricultural Marketing Service Perishable Agricultural Commodities Act; Request for Extension and Revision of a Currently Approved Information Collection AGENCY: Agricultural Marketing Service, USDA. ACTION: Notice and... 35), this document announces the Agricultural Marketing Service's (AMS) intention to request...

  15. 76 FR 40723 - Agency Information Collection Activities; Proposed Collection; Comment Request; Clean Water Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... AGENCY Agency Information Collection Activities; Proposed Collection; Comment Request; Clean Water Act... is planning to submit a request to renew an existing approved Information Collection Request (ICR) to... of the proposed information collection as described below. DATES: Comments must be submitted on...

  16. 75 FR 70160 - Affordable Care Act; Federal External Review Process; Request for Information

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... provisions of PHS Act section 2719 on July 23, 2010, at 75 FR 43330. Section 2719(b)(1) of the PHS Act and... Administration 29 CFR Part 2590 Affordable Care Act; Federal External Review Process; Request for Information... Act, as amended by the Affordable Care Act, and its implementing regulations. DATES: Submit written...

  17. 78 FR 27260 - Comment Request: Antarctic Conservation Act Application Permit Form

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... Comment Request: Antarctic Conservation Act Application Permit Form AGENCY: National Science Foundation...). SUPPLEMENTARY INFORMATION: Title of Collection: ``Antarctic Conservation Act Application Permit Form.'' OMB... approval to extend an information collection for three years. Proposed Project: The current...

  18. 12 CFR 1204.4 - How will FHFA respond to my Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... PRIVACY ACT IMPLEMENTATION § 1204.4 How will FHFA respond to my Privacy Act request? (a) How will FHFA... may disclose records or information to you directly with a written record made of the grant of the request. If you are to be accompanied by another person when accessing your record or any...

  19. Effects of Culture and Gender in Comprehension of Speech Acts of Indirect Request

    ERIC Educational Resources Information Center

    Shams, Rabe'a; Afghari, Akbar

    2011-01-01

    This study investigates the comprehension of indirect request speech act used by Iranian people in daily communication. The study is an attempt to find out whether different cultural backgrounds and the gender of the speakers affect the comprehension of the indirect request of speech act. The sample includes thirty males and females in Gachsaran…

  20. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Maintenance of Meeting Records § 1703.403 Requests for records under Freedom of Information and Privacy Acts... 45 Public Welfare 4 2014-10-01 2014-10-01 false Requests for records under Freedom of...

  1. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Maintenance of Meeting Records § 1703.403 Requests for records under Freedom of Information and Privacy Acts... 45 Public Welfare 4 2012-10-01 2012-10-01 false Requests for records under Freedom of...

  2. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Maintenance of Meeting Records § 1703.403 Requests for records under Freedom of Information and Privacy Acts... 45 Public Welfare 4 2013-10-01 2013-10-01 false Requests for records under Freedom of...

  3. 38 CFR 20.1200 - Rule 1200. Privacy Act request-appeal pending.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Rule 1200. Privacy Act... VETERANS AFFAIRS (CONTINUED) BOARD OF VETERANS' APPEALS: RULES OF PRACTICE Privacy Act § 20.1200 Rule 1200. Privacy Act request—appeal pending. When a Privacy Act request is filed under § 1.577 of this chapter...

  4. 76 FR 9611 - Notice of Permit Modification Request Received Under the Antarctic Conservation Act of 1978

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-18

    ... Conservation Act of 1978, Public Law 95-541. SUMMARY: Notice is hereby given that the National Science... Notice of Permit Modification Request Received Under the Antarctic Conservation Act of 1978 AGENCY... Antarctic Conservation Act of 1978 (Pub. L. 95-541; Code of Federal Regulations Title 45, Part 670). ]...

  5. 40 CFR 1515.5 - How to make a Freedom of Information Act request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false How to make a Freedom of Information... OF INFORMATION ACT PROCEDURES Procedures for Requesting Records § 1515.5 How to make a Freedom of... Information Officer shall decide how to respond to—or “make an initial determination on”—your request...

  6. 7 CFR 1700.3 - Requests under the Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Information Act are found at 7 CFR part 1. Requests must be in writing and may be submitted in person or by... Independence Avenue, SW., STOP 1530, Washington, DC 20250-1530; or by FAX to 202-401-1977. As set forth in 7 CFR 1.16, fees may be charged for processing of requests for records. An appeal of the...

  7. 76 FR 58277 - Animal Generic Drug User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-20

    ... HUMAN SERVICES Food and Drug Administration Animal Generic Drug User Fee Act; Public Meeting; Request... comments. The Food and Drug Administration (FDA) is announcing a public meeting on the Animal Generic Drug... on the Internet at...

  8. 78 FR 24227 - Notice of Proposed Information Collection; Comment Request: Real Estate Settlement Procedures Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection; Comment Request: Real Estate Settlement... information: Title of Proposal: Real Estate Settlement Procedures Act (RESPA) Disclosures. OMB Control Number... 24 CFR part 3500, require real estate settlement service providers to give homebuyers...

  9. Requests Made under the Official Information Act 1982: A Survey at the Agency Level.

    ERIC Educational Resources Information Center

    Clemens, Dave Andrew

    New Zealand's Official Information Act (OIA) has been in force for almost two decades. During these years, there has been little research published about how citizens use the legislation or how agencies have responded to requests made under the Act. The aim of this project is to produce basic research data from a range of agencies to quantify use…

  10. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Freedom of Information Act (FOIA) requests. 600.155 Section 600.155 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS...

  11. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Freedom of Information Act (FOIA) requests. 600.155 Section 600.155 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS...

  12. 75 FR 19669 - Andean Trade Preference Act (ATPA), as Amended: Request for Public Comments Regarding Beneficiary...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ... August 15, 2002 (67 FR 53379), for a full list of the eligibility criteria. 2. Requirements for... TRADE REPRESENTATIVE Andean Trade Preference Act (ATPA), as Amended: Request for Public Comments... submission) the ``USTR Report on Operation of the Andean Trade Preference Act.'' In order to be assured...

  13. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... send your written request to the Privacy Act Officer by electronic mail, regular mail, or fax. The electronic mail address is: privacy@fhfa.gov. The regular mail address is: Privacy Act Officer, Federal... the quickest possible handling, you should mark your electronic mail, letter, or fax and the...

  14. 77 FR 62494 - Proposed Information Collection; Comment Request; Evaluations of Coastal Zone Management Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-15

    ...; Evaluations of Coastal Zone Management Act Programs--State Coastal Management Programs and National Estuarine... . SUPPLEMENTARY INFORMATION: I. Abstract This request is for a new information collection. The Coastal Zone Management Act of 1972, as amended (CZMA; 16 U.S.C. 1451 et seq.) requires that state coastal...

  15. 28 CFR 513.61 - Freedom of Information Act requests by inmates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Freedom of Information Act requests by inmates. 513.61 Section 513.61 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act...

  16. Requests for euthanasia in general practice before and after implementation of the Dutch Euthanasia Act

    PubMed Central

    van Alphen, Jojanneke E; Donker, Gé A; Marquet, Richard L

    2010-01-01

    Background The Netherlands was the first country in the world to implement a Euthanasia Act in 2002. It is unknown whether legalising euthanasia under strict conditions influences the number and nature of euthanasia requests. Aim To investigate changes in the number of, and reasons for, requests for euthanasia in Dutch general practice after implementation of the Euthanasia Act. Design of study Retrospective dynamic cohort study comparing 5 years before (1998–2002) and 5 years after (2003–2007) implementation of the Act. Method Standardised registration forms were used to collect data on requests for euthanasia via the Dutch Sentinel Practice Network. This network of 45 general practices is nationally representative by age, sex, geographic distribution, and population density. Results The mean annual incidence of requests before implementation amounted to 3.1/10 000 and thereafter to 2.8/10 000 patients. However, trends differed by sex. The number of requests by males decreased significantly from 3.7/10 000 to 2.6/10 000 (P = 0.008); the requests by females increased non-significantly from 2.6/10 000 to 3.1/10 000. Before and after implementation, cancer remained the major underlying disease for requesting euthanasia: 82% versus 77% for men; 73% versus 75% for females. Pain was a major reason for a request, increasing in the period before implementation (mean 27%), but declining in the period thereafter (mean 22%). Loss of dignity became a less important reason after implementation (from 18% to 10%, P = 0.04), predominantly due to a marked decrease in the number of females citing it as a reason (from 17% to 6%, P = 0.02). Conclusion There was no increase in demand for euthanasia after implementation of the Euthanasia Act. Pain as a reason for requesting euthanasia showed an increasing trend before implementation, but declined thereafter. Loss of dignity as a reason declined, especially in females. PMID:20353671

  17. Watching Individual Proteins Acting on Single Molecules of DNA

    PubMed Central

    Amitani, Ichiro; Liu, Bian; Dombrowski, Christopher C.; Baskin, Ronald J.; Kowalczykowski, Stephen C.

    2011-01-01

    In traditional biochemical experiments, the behavior of individual proteins is obscured by ensemble averaging. To better understand the behavior of proteins that bind to and/or translocate on DNA, we have developed instrumentation that uses optical trapping, microfluidic solution delivery, and fluorescent microscopy to visualize either individual proteins or assemblies of proteins acting on single molecules of DNA. The general experimental design involves attaching a single DNA molecule to a polystyrene microsphere that is then used as a microscopic handle to manipulate individual DNA molecules with a laser trap. Visualization is achieved by fluorescently labeling either the DNA or the protein of interest, followed by direct imaging using high-sensitivity fluorescence microscopy. We describe the sample preparation and instrumentation used to visualize the interaction of individual proteins with single molecules of DNA. As examples, we describe the application of these methods to the study of proteins involved in recombination-mediated DNA repair, a process essential for the maintenance of genomic integrity. PMID:20580968

  18. Interlanguage Pragmatics in Russian: The Speech Act of Request in Email

    ERIC Educational Resources Information Center

    Krulatz, Anna M.

    2012-01-01

    As face-threatening speech acts, requests are of particular interest to second language acquisition scholars. They affect the interlocutors' public self-images, and thus require a careful consideration of the social distance between the interlocutors, their status, and the level of the imposition, factors that are weighed differently in…

  19. 76 FR 33413 - Proposed Renewal Without Change; Comment Request; Nine Bank Secrecy Act Recordkeeping Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... Financial Crimes Enforcement Network Proposed Renewal Without Change; Comment Request; Nine Bank Secrecy Act... be submitted to: Financial Crimes Enforcement Network, P.O. Box 39, Vienna, VA 22183, Attention: BSA... Requirements Comments.'' FOR FURTHER INFORMATION CONTACT: Financial Crimes Enforcement Network,...

  20. 29 CFR 1209.09 - Requests for records under Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....09 Section 1209.09 Labor Regulations Relating to Labor (Continued) NATIONAL MEDIATION BOARD PUBLIC OBSERVATION OF NATIONAL MEDIATION BOARD MEETINGS § 1209.09 Requests for records under Freedom of Information... 1208 of the Board's Rules addresses the requisite procedures under that Act....

  1. 76 FR 52295 - Amendment of Privacy Act Regulations, Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR 43 CFR Part 2 RIN 1090-AA94 Amendment of Privacy Act Regulations, Request for Comments AGENCY: Office... on October 3, 2011. ADDRESSES: Send written comments, identified by RIN 1090-AA94, by one of...

  2. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Requests for records under Freedom of Information and Privacy Acts. 1703.403 Section 1703.403 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE...

  3. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Requests for records under Freedom of Information and Privacy Acts. 1703.403 Section 1703.403 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE...

  4. 28 CFR 513.65 - Review of documents for Freedom of Information Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Review of documents for Freedom of Information Act requests. 513.65 Section 513.65 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom...

  5. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Freedom of Information Act and Privacy Act... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY INFORMATION SECURITY PROGRAM Declassification and Downgrading § 2400.22 Freedom of Information Act and...

  6. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Freedom of Information Act and Privacy Act... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY INFORMATION SECURITY PROGRAM Declassification and Downgrading § 2400.22 Freedom of Information Act and...

  7. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Tribal requests for additional Clean... TRIBAL CLEAN AIR ACT AUTHORITY Tribal Authority § 49.5 Tribal requests for additional Clean Air Act... request that the Administrator specify additional provisions of the Clean Air Act for which it would...

  8. Small circular DNA molecules act as rigid motifs to build DNA nanotubes.

    PubMed

    Zheng, Hongning; Xiao, Minyu; Yan, Qin; Ma, Yinzhou; Xiao, Shou-Jun

    2014-07-23

    Small circular DNA molecules with designed lengths, for example 64 and 96 nucleotides (nt), after hybridization with a few 32-nt staple strands respectively, can act as rigid motifs for the construction of DNA nanotubes with excellent uniformity in ring diameter. Unlike most native DNA nanotubes, which consist of longitudinal double helices, nanotubes assembled from circular DNAs are constructed from lateral double helices. Of the five types of DNA nanotubes designed here, four are built by alternating two different rings of the same ring size, while one is composed of all the same 96-nt rings. Nanotubes constructed from the same 96-nt rings are 10-100 times shorter than those constructed from two different 96-nt rings, because there are fewer hinge joints on the rings.

  9. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  10. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  11. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE TRIBAL CLEAN AIR ACT AUTHORITY Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act program...

  12. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  13. 3 CFR - State of California Request for Waiver Under 42 U.S.C. 7543(b), the Clean Air Act

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....C. 7543(b), the Clean Air Act Presidential Documents Other Presidential Documents Memorandum of January 26, 2009 State of California Request for Waiver Under 42 U.S.C. 7543(b), the Clean Air Act Memorandum for the Administrator of the Environmental Protection Agency Under the Clean Air Act (42...

  14. 77 FR 33388 - Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-06

    ... Animal and Plant Health Inspection Service Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration Requirement; Plants and Plant Products AGENCY: Animal and Plant Health... Plant Health Inspection Service's intention to request an extension of approval of an...

  15. 17 CFR Appendix A to Part 146 - Fees for Copies of Records Requested Under the Privacy Act of 1974

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 1 2011-04-01 2011-04-01 false Fees for Copies of Records Requested Under the Privacy Act of 1974 A Appendix A to Part 146 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION RECORDS MAINTAINED ON INDIVIDUALS Pt. 146, App. A Appendix A to Part 146—Fees for Copies of Records Requested...

  16. 78 FR 15019 - Food and Drug Administration Prescription Drug User Fee Act V Benefit-Risk Plan; Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... HUMAN SERVICES Food and Drug Administration Food and Drug Administration Prescription Drug User Fee Act V Benefit-Risk Plan; Request for Comments AGENCY: Food and Drug Administration, HHS. ACTION: Notice, request for comments. SUMMARY: The Food and Drug Administration (FDA or the Agency) is announcing...

  17. 12 CFR 1204.4 - How will FHFA or FHFA-OIG respond to my Privacy Act request?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 9 2012-01-01 2012-01-01 false How will FHFA or FHFA-OIG respond to my Privacy Act request? 1204.4 Section 1204.4 Banks and Banking FEDERAL HOUSING FINANCE AGENCY ORGANIZATION AND... amendment or correction of a record in whole or in part; (3) Declines to provide a requested accounting...

  18. The Effect of Explicit Metapragmatic Instruction on Request Speech Act Awareness of Intermediate EFL Students at Institute Level

    ERIC Educational Resources Information Center

    Masouleh, Fatemeh Abdollahizadeh; Arjmandi, Masoumeh; Vahdany, Fereydoon

    2014-01-01

    This study deals with the application of the pragmatics research to EFL teaching. The need for language learners to utilize a form of speech acts such as request which involves a series of strategies was significance of the study. Although defining different speech acts has been established since 1960s, recently there has been a shift towards…

  19. 78 FR 23885 - Federal Seed Act Program; Request for Extension and Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-23

    ... Agricultural Marketing Service Federal Seed Act Program; Request for Extension and Revision of a Currently... collection 7 CFR part 201 for Federal Seed Act Labeling and Enforcement. DATES: Comments on this document... submitted online at www.regulations.gov or sent to Fawad S. Shah, Director, Seed Regulatory and...

  20. 12 CFR 1204.5 - What if I am dissatisfied with the FHFA response to my Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false What if I am dissatisfied with the FHFA response to my Privacy Act request? 1204.5 Section 1204.5 Banks and Banking FEDERAL HOUSING FINANCE AGENCY... explain your reason(s) for disagreeing with the Privacy Act Appeals Officer's denial in whole or in...

  1. DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair

    PubMed Central

    Abdou, Ismail; Poirier, Guy G.; Hendzel, Michael J.; Weinfeld, Michael

    2015-01-01

    In the current model of DNA SSBR, PARP1 is regarded as the sensor of single-strand breaks (SSBs). However, biochemical studies have implicated LIG3 as another possible SSB sensor. Using a laser micro-irradiation protocol that predominantly generates SSBs, we were able to demonstrate that PARP1 is dispensable for the accumulation of different single-strand break repair (SSBR) proteins at sites of DNA damage in live cells. Furthermore, we show in live cells for the first time that LIG3 plays a role in mediating the accumulation of the SSBR proteins XRCC1 and PNKP at sites of DNA damage. Importantly, the accumulation of LIG3 at sites of DNA damage did not require the BRCT domain-mediated interaction with XRCC1. We were able to show that the N-terminal ZnF domain of LIG3 plays a key role in the enzyme's SSB sensing function. Finally, we provide cellular evidence that LIG3 and not PARP1 acts as the sensor for DNA damage caused by the topoisomerase I inhibitor, irinotecan. Our results support the existence of a second damage-sensing mechanism in SSBR involving the detection of nicks in the genome by LIG3. PMID:25539916

  2. 8 CFR 103.10 - Requests for records under the Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., district offices, and sub-offices see 8 CFR 100.4. (2) Manner of requesting records. All Freedom of... under 28 CFR 16.1(d). A request improperly addressed will not be deemed to have been received for..., requesters may deem their request to be denied and exercise their right to appeal in accordance with 28...

  3. 75 FR 15458 - Request for Small Reclamation Projects Act Loan To Construct Narrows Dam in Sanpete County, UT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... 25, 2003 (68 FR 66123-66124). The SDEIS describes and analyzes the potential effects of three action... Bureau of Reclamation Request for Small Reclamation Projects Act Loan To Construct Narrows Dam in Sanpete... law. These Reclamation actions would facilitate the construction by SWCD of the proposed Narrows...

  4. 76 FR 40728 - Clean Air Act Advisory Committee (CAAAC); Request for Nominations for 2011 Clean Air Excellence...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... AGENCY Clean Air Act Advisory Committee (CAAAC); Request for Nominations for 2011 Clean Air Excellence... Air Excellence Awards. ] SUMMARY: EPA established the Clean Air Excellence Awards Program in February... progress in achieving clean air. This notice announces the competition for the Year 2011 program....

  5. 75 FR 35025 - Clean Air Act Advisory Committee (CAAAC); Request for Nominations for 2010 Clean Air Excellence...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... AGENCY Clean Air Act Advisory Committee (CAAAC); Request for Nominations for 2010 Clean Air Excellence... Air Excellence Awards. SUMMARY: EPA established the Clean Air Excellence Awards Program in February... progress in achieving clean air. This notice announces the competition for the Year 2010 program....

  6. 78 FR 40443 - Submission for OMB Review; Comment Request-Virginia Graeme Baker Pool and Spa Safety Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-05

    ... of April 19, 2013 (76 FR 23546), the Consumer Product Safety Commission (CPSC or Commission... COMMISSION Submission for OMB Review; Comment Request--Virginia Graeme Baker Pool and Spa Safety Act... verify whether pools and spas are in compliance with the Virginia Graeme Baker Pool and Spa Safety...

  7. Examination of Learner and Situation Level Variables: Choice of Speech Act and Request Strategy by Spanish L2 Learners

    ERIC Educational Resources Information Center

    Kuriscak, Lisa

    2015-01-01

    This study focuses on variation within a group of learners of Spanish (N = 253) who produced requests and complaints via a written discourse completion task. It examines the effects of learner and situational variables on production--the effect of proficiency and addressee-gender on speech-act choice and the effect of perception of imposition on…

  8. 28 CFR Appendix to Subpart H of... - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... by 28 CFR 16.5 (b) and (c) and 16.45(a), as amended July 1, 1977, and defined in those sections, for... 28 Judicial Administration 1 2014-07-01 2014-07-01 false Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H of Part 0...

  9. 28 CFR Appendix to Subpart H of... - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... by 28 CFR 16.5 (b) and (c) and 16.45(a), as amended July 1, 1977, and defined in those sections, for... 28 Judicial Administration 1 2013-07-01 2013-07-01 false Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H of Part 0...

  10. 28 CFR Appendix to Subpart H - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... duties and responsibilities previously assigned to the Assistant Attorney General by 28 CFR 16.5 (b) and... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H Judicial...

  11. 28 CFR Appendix to Subpart H - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... duties and responsibilities previously assigned to the Assistant Attorney General by 28 CFR 16.5 (b) and... 28 Judicial Administration 1 2012-07-01 2012-07-01 false Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H Judicial...

  12. 12 CFR 1204.5 - What if I am dissatisfied with the response to my Privacy Act request?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... my Privacy Act request? 1204.5 Section 1204.5 Banks and Banking FEDERAL HOUSING FINANCE AGENCY... Finance Agency, 1700 G Street, NW., Washington, DC 20552. The facsimile number is: (202) 414-8917. For... persons, organizations, or Federal agencies to which it previously disclosed the record, if an...

  13. 28 CFR Appendix to Subpart H - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... duties and responsibilities previously assigned to the Assistant Attorney General by 28 CFR 16.5 (b) and... Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H Judicial Administration DEPARTMENT OF JUSTICE ORGANIZATION OF THE DEPARTMENT OF JUSTICE Antitrust Division Pt. 0, Subpt. H,...

  14. 43 CFR 2.27 - How will a bureau handle a request for information that is contained in a Privacy Act system of...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... information that is contained in a Privacy Act system of records? (See DOI's Privacy Act regulations (Subpart... system of records? (See DOI's Privacy Act regulations (Subpart G of this part) for additional information.) (a) When you request information pertaining to yourself that is contained in a Privacy Act system...

  15. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Reading Room where CEQ makes available records pertaining to matters within the scope of 5 U.S.C. 552(a)(2... requesters to review documents currently available from the Center's Online Reading Room before submitting a... needed to process the request. CEQ may provide requesters on a slower track an opportunity to limit...

  16. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Reading Room where CEQ makes available records pertaining to matters within the scope of 5 U.S.C. 552(a)(2... requesters to review documents currently available from the Center's Online Reading Room before submitting a... needed to process the request. CEQ may provide requesters on a slower track an opportunity to limit...

  17. Male DNA typing from 25-year-old vaginal swabs using Y chromosomal STR polymorphisms in a retrial request case.

    PubMed

    Honda, K; Roewer, L; de Knijff, P

    1999-07-01

    We report here the application of Y chromosomal DNA analysis in a retrial request case, raised officially by Sapporo High Court, Japan, of a condemned criminal whose capital punishment has been suspended. DNA was extracted from mixed seminal/vaginal secretion stains collected 25 years ago from two raped and murdered victims, and Y chromosome STR loci (DYS19, 390, 393, YCAII) were amplified and sequenced to clarify the DNA type of the rapist. Alkaline proteinase and sodium hydroxide were used before phenol/chloroform extraction to achieve high quality DNA from very old samples. In addition, amplified fragments of DYS19, DYS390, and DYS393 were sequenced using an automated DNA sequencer. Four Y STR DNA types detected from vaginal swabs were found identical to those of the accused criminal and confirmed that the two rape and murder cases had been committed by the same person. Sapporo High Court accepted the results and rejected the retrial request in February 1998.

  18. [Cu(phen)2](2+) acts as electrochemical indicator and anchor to immobilize probe DNA in electrochemical DNA biosensor.

    PubMed

    Yang, Linlin; Li, Xiaoyu; Li, Xi; Yan, Songling; Ren, Yinna; Wang, Mengmeng; Liu, Peng; Dong, Yulin; Zhang, Chaocan

    2016-01-01

    We demonstrate a novel protocol for sensitive in situ label-free electrochemical detection of DNA hybridization based on copper complex ([Cu(phen)2](2+), where phen = 1,10-phenanthroline) and graphene (GR) modified glassy carbon electrode. Here, [Cu(phen)2](2+) acted advantageously as both the electrochemical indicator and the anchor for probe DNA immobilization via intercalative interactions between the partial double helix structure of probe DNA and the vertical aromatic groups of phen. GR provided large density of docking site for probe DNA immobilization and increased the electrical conductivity ability of the electrode. The modification procedure was monitored by electrochemical impedance spectroscopy (EIS). Square-wave voltammetry (SWV) was used to explore the hybridization events. Under the optimal conditions, the designed electrochemical DNA biosensor could effectively distinguish different mismatch degrees of complementary DNA from one-base mismatch to noncomplementary, indicating that the biosensor had high selectivity. It also exhibited a reasonable linear relationship. The oxidation peak currents of [Cu(phen)2](2+) were linear with the logarithm of the concentrations of complementary target DNA ranging from 1 × 10(-12) to 1 × 10(-6) M with a detection limit of 1.99 × 10(-13) M (signal/noise = 3). Moreover, the stability of the electrochemical DNA biosensor was also studied.

  19. [Cu(phen)2](2+) acts as electrochemical indicator and anchor to immobilize probe DNA in electrochemical DNA biosensor.

    PubMed

    Yang, Linlin; Li, Xiaoyu; Li, Xi; Yan, Songling; Ren, Yinna; Wang, Mengmeng; Liu, Peng; Dong, Yulin; Zhang, Chaocan

    2016-01-01

    We demonstrate a novel protocol for sensitive in situ label-free electrochemical detection of DNA hybridization based on copper complex ([Cu(phen)2](2+), where phen = 1,10-phenanthroline) and graphene (GR) modified glassy carbon electrode. Here, [Cu(phen)2](2+) acted advantageously as both the electrochemical indicator and the anchor for probe DNA immobilization via intercalative interactions between the partial double helix structure of probe DNA and the vertical aromatic groups of phen. GR provided large density of docking site for probe DNA immobilization and increased the electrical conductivity ability of the electrode. The modification procedure was monitored by electrochemical impedance spectroscopy (EIS). Square-wave voltammetry (SWV) was used to explore the hybridization events. Under the optimal conditions, the designed electrochemical DNA biosensor could effectively distinguish different mismatch degrees of complementary DNA from one-base mismatch to noncomplementary, indicating that the biosensor had high selectivity. It also exhibited a reasonable linear relationship. The oxidation peak currents of [Cu(phen)2](2+) were linear with the logarithm of the concentrations of complementary target DNA ranging from 1 × 10(-12) to 1 × 10(-6) M with a detection limit of 1.99 × 10(-13) M (signal/noise = 3). Moreover, the stability of the electrochemical DNA biosensor was also studied. PMID:26403602

  20. DNA Identification Act of 1993. House of Representatives, One Hundred Third Congress, First Session

    SciTech Connect

    Not Available

    1993-01-01

    Report 103-45 on the DNA Identification Act of 1993 is intended to amend H.R. 829 (to amend title I of the Omnibus Crime Control and Safe Streets Act of 1968 to authorize funds received by States and units of the local government to be expended to improve the quality and availability of DNA records; to authorize the establishment of a DNA identification index; and for other purposes). The report states that the Committee on the Judiciary recommends Bill H.R. 829 pass with the amendment contained in this report. This report explains the amendment, the committee vote on H.R. 829, background behind H.R. 829, quality control concerns, proficincy testing, the significance of DNA matches, and databanking and privacy. Also included is a section-by-section analysis of H.R. 829 and committee oversight findings.

  1. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...), as amended, and environmental issues and other documents that, because of the nature of their subject... request. 1515.5 Section 1515.5 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY FREEDOM OF....” Written requests may also be faxed to (202) 456-0753 or addressed and mailed to: Council on...

  2. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...), as amended, and environmental issues and other documents that, because of the nature of their subject... request. 1515.5 Section 1515.5 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY FREEDOM OF....” Written requests may also be faxed to (202) 456-0753 or addressed and mailed to: Council on...

  3. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Tribal requests for additional Clean... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air...

  4. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... of identity, preferably a government-issued document bearing the individual's photograph. (d) Freedom... or name, author, recipient, and subject matter. (c) Proof of identity. Requests received by mail,...

  5. DIRECT-ACTING, DNA-DAMAGING AS (III)-METHYLATED SPECIES: IMPLICATIONS FOR A CARCINOGENIC MECHANISM OF ACTION OF ARSENICALS

    EPA Science Inventory

    Direct-acting, DNA-damaging As (III)-methylated species: implications for a carcinogenic . mechanism of action of arsenicals

    Inorganic arsenic (iAs, arsenite and arsenate) has been thought to act as a carcinogen without reacting directly with DNA; neither iAs nor the As(...

  6. DNA evidence in rape cases and the Debbie Smith Act: forensic practice and criminal justice implications.

    PubMed

    Telsavaara, Terhi V T; Arrigo, Bruce A

    2006-10-01

    The Debbie Smith or "Justice for All" Act was passed on November 1, 2004. The act addresses the problem of collecting and analyzing DNA evidence from backlogged rape kits sitting in crime laboratories around the country. Presently, no empirical data exist by which to assess the soundness of the legislation. However, the act clearly affects discrete operations within the forensic and criminal justice systems. This article explores the relative merits of the Debbie Smith law, highlighting changes in Sexual Assault Nurse Examiner (SANE) programs, law enforcement, court administration, correctional treatment, and juvenile justice practices. Concerns linked to the likely impact of the "Justice for All" Act raise significant questions about its overall programmatic utility and treatment efficacy.

  7. 77 FR 61008 - Request for Comments Under the Paperwork Reduction Act, Section 3506

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-05

    ... Carr, Acting Director, Office of Clinical Research and Bioethics Policy, Office of Science Policy, NIH... 28, 2012. Sarah Carr, Acting Director, Office of Clinical Research and Bioethics Policy, Office...

  8. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Officer by electronic mail, mail, delivery service, or facsimile. The electronic mail address is: privacy... can help FHFA and FHFA-OIG process your request by marking electronic mail, letters, or facsimiles and..., passport card, driver's license, ID card, etc.), or one form of unexpired identification with a...

  9. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Officer by electronic mail, mail, delivery service, or facsimile. The electronic mail address is: privacy... direct response. You can help FHFA and FHFA-OIG process your request by marking electronic mail, letters... agency or entity (i.e. passport, passport card, driver's license, ID card, etc.), or one form...

  10. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Officer by electronic mail, mail, delivery service, or facsimile. The electronic mail address is: privacy... direct response. You can help FHFA and FHFA-OIG process your request by marking electronic mail, letters... agency or entity (i.e. passport, passport card, driver's license, ID card, etc.), or one form...

  11. Does Instruction Alter the Naturalistic Pattern of Pragmatic Development? A Case of Request Speech Act

    ERIC Educational Resources Information Center

    Taguchi, Naoko; Naganuma, Naeko; Budding, Carlos

    2015-01-01

    This study examined the effects of explicit instruction on the development of pragmatic competence in L2 English. The study is based on Taguchi's (2012) study conducted in an English-medium university in Japan, which revealed patterns of change in Japanese EFL students' production of requests in high- and low-imposition situations. Students showed…

  12. 75 FR 32483 - Prescription Drug User Fee Act; Meetings on Reauthorization; Request for Notification of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ... reauthorization (75 FR 12555, March 16, 2010). This meeting and written comments submitted to the docket have...; Request for Notification of Stakeholder Intention to Participate AGENCY: Food and Drug Administration, HHS... by establishing consistent stakeholder representation. DATES: Submit notification of intention...

  13. Clofarabine Acts as Radiosensitizer In Vitro and In Vivo by Interfering With DNA Damage Response

    SciTech Connect

    Cariveau, Mickael J.; Stackhouse, Murray; Cui Xiaoli; Tiwari, Kamal; Waud, William; Secrist, John A.; Xu Bo

    2008-01-01

    Purpose: Combination treatment with radiotherapy and chemotherapy has emerged as the dominant form of cancer adjuvant regimens in recent years. Clofarabine, a newly approved drug for pediatric leukemia, is a second-generation purine nucleoside analogue that can block DNA synthesis and inhibit DNA repair. Therefore, we hypothesized that clofarabine could work synergistically with radiotherapy to increase the tumor cell response. Methods and Materials: The effects of clofarabine on radiosensitivity have been established in several tumor cell lines in vitro and in vivo using colony-forming assays and tumor xenografts. The effect of clofarabine on the DNA damage response was also studied in vitro by measuring {gamma}-H2AX focus formation. Results: Clonogenic survival was significantly reduced in irradiated cells treated with clofarabine, demonstrating the strong radiosensitizing effect of clofarabine. Furthermore, clofarabine displayed a radiosensitizing effect that was greater than gemcitabine or 5-fluorouracil. We also found that low doses of clofarabine can prolong the presence of radiation-induced {gamma}-H2AX nuclear focus formation, and high doses of clofarabine can induce DNA double-strand breaks, suggesting that clofarabine can interfere with DNA damage response pathways. In addition, clofarabine-induced radiosensitization was also established in vivo using a colorectal cancer model, DLD-1, in athymic nude mice. When combined with fractionated radiotherapy, a moderate dose of clofarabine led to a significant increase in tumor growth inhibition. Conclusion: Clofarabine acts as a powerful radiosensitizer both in vitro and in vivo by interfering with the DNA damage response.

  14. 77 FR 37923 - Comment Request for Information Collection for the Workforce Investment Act (WIA) Adult and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-25

    ... Investment Act (WIA) Adult and Dislocated Worker Programs Gold Standard Evaluation (WIA Evaluation); New...: New collection. Title of Collection: WIA Adult and Dislocated Worker Programs Gold Standard...

  15. 75 FR 12496 - Proposed Information Collection; Comment Request; DOC National Environmental Policy Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... Policy Act Environmental Questionnaire and Checklist AGENCY: Office of the Secretary, Office of...) Environmental Questionnaire and Checklist (EQC) was developed to assist DOC in complying with NEPA...

  16. Cis-acting signals modulate the efficiency of programmed DNA elimination in Paramecium tetraurelia.

    PubMed

    Ferro, Diana; Lepennetier, Gildas; Catania, Francesco

    2015-09-30

    In Paramecium, the regeneration of a functional somatic genome at each sexual event relies on the elimination of thousands of germline DNA sequences, known as Internal Eliminated Sequences (IESs), from the zygotic nuclear DNA. Here, we provide evidence that IESs' length and sub-terminal bases jointly modulate IES excision by affecting DNA conformation in P. tetraurelia. Our study reveals an excess of complementary base pairing between IESs' sub-terminal and contiguous sites, suggesting that IESs may form DNA loops prior to cleavage. The degree of complementary base pairing between IESs' sub-terminal sites (termed Cin-score) is positively associated with IES length and is shaped by natural selection. Moreover, it escalates abruptly when IES length exceeds 45 nucleotides (nt), indicating that only sufficiently large IESs may form loops. Finally, we find that IESs smaller than 46 nt are favored targets of the cellular surveillance systems, presumably because of their relatively inefficient excision. Our findings extend the repertoire of cis-acting determinants for IES recognition/excision and provide unprecedented insights into the distinct selective pressures that operate on IESs and somatic DNA regions. This information potentially moves current models of IES evolution and of mechanisms of IES recognition/excision forward. PMID:26304543

  17. Cis-acting signals modulate the efficiency of programmed DNA elimination in Paramecium tetraurelia.

    PubMed

    Ferro, Diana; Lepennetier, Gildas; Catania, Francesco

    2015-09-30

    In Paramecium, the regeneration of a functional somatic genome at each sexual event relies on the elimination of thousands of germline DNA sequences, known as Internal Eliminated Sequences (IESs), from the zygotic nuclear DNA. Here, we provide evidence that IESs' length and sub-terminal bases jointly modulate IES excision by affecting DNA conformation in P. tetraurelia. Our study reveals an excess of complementary base pairing between IESs' sub-terminal and contiguous sites, suggesting that IESs may form DNA loops prior to cleavage. The degree of complementary base pairing between IESs' sub-terminal sites (termed Cin-score) is positively associated with IES length and is shaped by natural selection. Moreover, it escalates abruptly when IES length exceeds 45 nucleotides (nt), indicating that only sufficiently large IESs may form loops. Finally, we find that IESs smaller than 46 nt are favored targets of the cellular surveillance systems, presumably because of their relatively inefficient excision. Our findings extend the repertoire of cis-acting determinants for IES recognition/excision and provide unprecedented insights into the distinct selective pressures that operate on IESs and somatic DNA regions. This information potentially moves current models of IES evolution and of mechanisms of IES recognition/excision forward.

  18. 77 FR 4564 - Request for Information Regarding the Reinsurance Program Under the Affordable Care Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-30

    ..., 2011 (76 FR 41930). The Affordable Care Act instructs each State to establish or contract with an... hotlines, helpdesk)? 21. Are your support systems compliant or have the capability of being Section...

  19. 76 FR 18254 - Proposed Information Collection Request (ICR) for the Family Medical Leave Act (FMLA) Employee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... qualitative data obtained provided a detailed anecdotal picture of the workings of the FMLA. The period for... Paperwork Reduction Act of 1995 (PRA95) . This program helps to ensure that required data can be provided in... and to close current data gaps remaining from the previous surveys. To better understand...

  20. 77 FR 24555 - Andean Trade Preference Act (ATPA), as Amended: Request for Public Comments Regarding Beneficiary...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-24

    ... Register notice published on August 15, 2002 (67 FR 53379), for a full list of the eligibility criteria... interested parties on whether the remaining designated beneficiary country (as of May 15, 2012), Ecuador, is...., provides trade benefits for eligible Andean countries. The original Act allowed only Bolivia,...

  1. 77 FR 59224 - Comment Request for Information Collection for the Workforce Investment Act (WIA) Management...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    ... Investment Act (WIA) Management Information and Reporting System; Extension With Revisions AGENCY: Employment... comments concerning the collection of data about the WIA Management Information and Reporting System (OMB... revisions to the WIA Management Information and Reporting System. The first are changes to the...

  2. Job Training Partnership Act: Native American Status for American Samoans Appears Unwarranted. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    General Accounting Office, Washington, DC. Div. of Human Resources.

    This report is the result of a study to assess whether American Samoans should be designated as Native Americans under the Job Training Partnership Act. The finding is that there is insufficient evidence in favor of such a change in designation. To designate American Samoans as Native Americans may set a precedent for amending numerous other…

  3. 78 FR 27187 - Proposed Information Collection; Comment Request; Green Sturgeon Endangered Species Act Take...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... scientific research, scientific monitoring, and fisheries activities conducted under an approved 4(d) program... Species Act (ESA) were promulgated for the species on June 2, 2010 (75 FR 30714) (the final ESA 4(d) Rule... Respondents: 67. Estimated Time per Response: Written notification describing research, monitoring or...

  4. 77 FR 57544 - Native American Housing Assistance and Self-Determination Reauthorization Act of 1996: Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-18

    ... URBAN DEVELOPMENT 24 CFR Part 1000 Native American Housing Assistance and Self-Determination... Housing Block Grant program authorized by the Native American Housing Assistance and Self-Determination...). SUPPLEMENTARY INFORMATION: I. Background The Native American Housing Assistance and Self-Determination Act...

  5. 75 FR 5346 - Comment Request for Information Collection for Workforce Investment Act National Emergency Grant...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-02

    .... III. Current Actions: Type of Review: Extension without changes. Title: Workforce Investment Act...: Employment and Training Administration. ACTION: Notice. SUMMARY: The Department of Labor, as part of its... Regulations define four NEG project types: Regular, which encompasses plant closures, mass layoffs,...

  6. 77 FR 59899 - Proposed Information Collection; Comment Request; Coastal Zone Management Act Walter B. Jones and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-01

    ... Zone Management Act Walter B. Jones and NOAA Excellence Awards AGENCY: National Oceanic and Atmospheric... promote excellence in coastal zone management by identifying and acknowledging outstanding accomplishments... recognize three categories of excellence: Coastal Steward of the Year, Excellence in Local Government,...

  7. 10 CFR 905.23 - What are the opportunities for using the Freedom of Information Act to request plan and report data?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false What are the opportunities for using the Freedom of Information Act to request plan and report data? 905.23 Section 905.23 Energy DEPARTMENT OF ENERGY ENERGY PLANNING AND MANAGEMENT PROGRAM Integrated Resource Planning § 905.23 What are the opportunities for...

  8. Assessment of ADHD Documentation from Candidates Requesting Americans with Disabilities Act (ADA) Accommodations for the National Board of Osteopathic Medical Examiners COMLEX Exam

    ERIC Educational Resources Information Center

    Joy, Javed A.; Julius, Rose J.; Akter, Rashida; Baron, David A.

    2010-01-01

    Purpose: Every year increasing numbers of candidates request special accommodations for high-stakes medical licensing examinations, due to ADHD, on the basis of the Americans with Disabilities Act (ADA). This poses significant challenges for both the applicant and the medical boards and has significant financial, legal, and ethical implications.…

  9. 15 CFR 781.4 - U.S. Government requests for information needed to satisfy the requirements of the APR or the Act.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false U.S. Government requests for information needed to satisfy the requirements of the APR or the Act. 781.4 Section 781.4 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND...

  10. DNA repair after ultraviolet irradiation of ICR 2A frog cells: pyrimidine dimers are long acting blocks to nascent DNA synthesis

    SciTech Connect

    Rosenstein, B.S.; Setlow, R.B.

    1980-08-01

    The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecular weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.

  11. A novel cis-acting element required for DNA damage-inducible expression of yeast DIN7

    SciTech Connect

    Yoshitani, Ayako; Yoshida, Minoru; Ling Feng

    2008-01-04

    Din7 is a DNA damage-inducible mitochondrial nuclease that modulates the stability of mitochondrial DNA (mtDNA) in Saccharomyces cerevisiae. How DIN7 gene expression is regulated, however, has remained largely unclear. Using promoter sequence alignment, we found a highly conserved 19-bp sequence in the promoter regions of DIN7 and NTG1, which encodes an oxidative stress-inducible base-excision-repair enzyme. Deletion of the 19-bp sequence markedly reduced the hydroxyurea (HU)-enhanced DIN7 promoter activity. In addition, nuclear fractions prepared from HU-treated cells were used in in vitro band shift assays to reveal the presence of currently unidentified trans-acting factor(s) that preferentially bound to the 19-bp region. These results suggest that the 19-bp sequence is a novel cis-acting element that is required for the regulation of DIN7 expression in response to HU-induced DNA damage.

  12. DOE FY 2010 Budget Request and Recovery Act Funding for Energy Research, Development, Demonstration, and Deployment: Analysis and Recommendations

    SciTech Connect

    Anadon, Laura Diaz; Gallagher, Kelly Sims; Bunn, Matthew

    2009-06-01

    The combination of the FY 2010 budget request for the Department of Energy (DOE) and the portion of the American Recovery and Reinvestment Act of 2009 (ARRA) funds likely to be available in 2010 would (assuming that they would be split evenly between FY 2010 and FY 2011) result in a doubling in funding available for energy research, development, and deployment (ERD and D) from $3.6 billion in FY 2009 to $7.2 billion in FY 2010. Without the stimulus funds, DOE ERD and D investments in FY 2010 would decrease very slightly when compared to FY 2009. Excluding the $7.5 billion for the Advanced Technology Vehicles Manufacturing Loans in FY 2009, the FY 2010 budget request for deployment represents a 33 percent decrease from the FY 2009 levels from $520 million to $350 million. This decrease is largely due to the large amounts of funds appropriated in ARRA for DOE deployment programs, or $23.6 billion, which are three times greater than those appropriated in the FY 2009 budget. These very substantial funding amounts, coupled with the broad range of institutional innovations the administration is putting in place and movement toward putting a price on carbon emissions, will help accelerate innovation for a broad range of energy technologies. DOE's Advanced Research Projects Agency-Energy (ARPA-E) and the Energy Innovation Hubs are important initiatives that could contribute to two weak points of the government's energy innovation effort, namely funding high-risk projects in transformational technologies and in companies that have not traditionally worked with the government and strengthening the integration of basic and applied research in priority areas. Increasing the funding for different types of energy storage research, providing some support for exploring opportunities in coal-to-liquids with carbon capture and storage (CCS) and coal-and-biomass-to-liquids with CCS, and reducing funding for fission RD and D are other actions that Congress could take in the short

  13. Two high-mobility group box domains act together to underwind and kink DNA

    SciTech Connect

    Sánchez-Giraldo, R.; Acosta-Reyes, F. J.; Malarkey, C. S.; Saperas, N.; Churchill, M. E. A.; Campos, J. L.

    2015-06-30

    The crystal structure of HMGB1 box A bound to an unmodified AT-rich DNA fragment is reported at a resolution of 2 Å. A new mode of DNA recognition for HMG box proteins is found in which two box A domains bind in an unusual configuration generating a highly kinked DNA structure. High-mobility group protein 1 (HMGB1) is an essential and ubiquitous DNA architectural factor that influences a myriad of cellular processes. HMGB1 contains two DNA-binding domains, box A and box B, which have little sequence specificity but have remarkable abilities to underwind and bend DNA. Although HMGB1 box A is thought to be responsible for the majority of HMGB1–DNA interactions with pre-bent or kinked DNA, little is known about how it recognizes unmodified DNA. Here, the crystal structure of HMGB1 box A bound to an AT-rich DNA fragment is reported at a resolution of 2 Å. Two box A domains of HMGB1 collaborate in an unusual configuration in which the Phe37 residues of both domains stack together and intercalate the same CG base pair, generating highly kinked DNA. This represents a novel mode of DNA recognition for HMGB proteins and reveals a mechanism by which structure-specific HMG boxes kink linear DNA.

  14. DNA Ligase IV and Artemis Act Cooperatively to Suppress Homologous Recombination in Human Cells: Implications for DNA Double-Strand Break Repair

    PubMed Central

    Kurosawa, Aya; Saito, Shinta; So, Sairei; Hashimoto, Mitsumasa; Iwabuchi, Kuniyoshi; Watabe, Haruka; Adachi, Noritaka

    2013-01-01

    Nonhomologous end-joining (NHEJ) and homologous recombination (HR) are two major pathways for repairing DNA double-strand breaks (DSBs); however, their respective roles in human somatic cells remain to be elucidated. Here we show using a series of human gene-knockout cell lines that NHEJ repairs nearly all of the topoisomerase II- and low-dose radiation-induced DNA damage, while it negatively affects survival of cells harbouring replication-associated DSBs. Intriguingly, we find that loss of DNA ligase IV, a critical NHEJ ligase, and Artemis, an NHEJ factor with endonuclease activity, independently contribute to increased resistance to replication-associated DSBs. We also show that loss of Artemis alleviates hypersensitivity of DNA ligase IV-null cells to low-dose radiation- and topoisomerase II-induced DSBs. Finally, we demonstrate that Artemis-null human cells display increased gene-targeting efficiencies, particularly in the absence of DNA ligase IV. Collectively, these data suggest that DNA ligase IV and Artemis act cooperatively to promote NHEJ, thereby suppressing HR. Our results point to the possibility that HR can only operate on accidental DSBs when NHEJ is missing or abortive, and Artemis may be involved in pathway switching from incomplete NHEJ to HR. PMID:23967291

  15. Single-stranded DNA fragments of insect-specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control.

    PubMed

    Oberemok, Volodymyr V; Skorokhod, Oleksii A

    2014-07-01

    This paper focuses on the DNA insecticides as a novel preparation against gypsy moth (Lymantria dispar) based on DNA fragments of the anti-apoptotic gene of its nuclear polyhedrosis virus. It was found that the external application of a solution with two single-stranded DNA fragments from BIR and RING domains of LdMNPV (L.dispar multicapsid nuclear polyhedrosis virus) IAP-3 (inhibitor of apoptosis) gene induces a significantly higher mortality of gypsy moth caterpillars in comparison with the application of the control solutions. This effect does not depend on the infection of caterpillars with LdMNPV. The results also show that DNA insecticides based on LdMNPV IAP-3 gene fragments can be selective in action, and at least are not harmful to tobacco hornworm (Manduca sexta) and black cutworm (Agrotis ipsilon). Part of the gypsy moth genome cloned with the fragments of BIR and RING domains of LdMNPV IAP-3 gene as primers, has an overlap with the corresponding part of the LdMNPV IAP-3 gene and L.dispar IAP-1 mRNA for an inhibitor of apoptosis protein with the high cover by query, allows assuming that we cloned a part of gypsy moth anti-apoptosis gene. This finding gives the grounding that proposed here DNA insecticides might act through the blocking of the mechanisms involved in post transcriptional expression of insect anti-apoptosis genes. The results show the insecticidal potential of the viral genome fragments that can be used to create safe and relatively fast-acting DNA insecticides to control the quantity of gypsy moth populations, important task for forestry and agriculture.

  16. Single-stranded DNA fragments of insect-specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control.

    PubMed

    Oberemok, Volodymyr V; Skorokhod, Oleksii A

    2014-07-01

    This paper focuses on the DNA insecticides as a novel preparation against gypsy moth (Lymantria dispar) based on DNA fragments of the anti-apoptotic gene of its nuclear polyhedrosis virus. It was found that the external application of a solution with two single-stranded DNA fragments from BIR and RING domains of LdMNPV (L.dispar multicapsid nuclear polyhedrosis virus) IAP-3 (inhibitor of apoptosis) gene induces a significantly higher mortality of gypsy moth caterpillars in comparison with the application of the control solutions. This effect does not depend on the infection of caterpillars with LdMNPV. The results also show that DNA insecticides based on LdMNPV IAP-3 gene fragments can be selective in action, and at least are not harmful to tobacco hornworm (Manduca sexta) and black cutworm (Agrotis ipsilon). Part of the gypsy moth genome cloned with the fragments of BIR and RING domains of LdMNPV IAP-3 gene as primers, has an overlap with the corresponding part of the LdMNPV IAP-3 gene and L.dispar IAP-1 mRNA for an inhibitor of apoptosis protein with the high cover by query, allows assuming that we cloned a part of gypsy moth anti-apoptosis gene. This finding gives the grounding that proposed here DNA insecticides might act through the blocking of the mechanisms involved in post transcriptional expression of insect anti-apoptosis genes. The results show the insecticidal potential of the viral genome fragments that can be used to create safe and relatively fast-acting DNA insecticides to control the quantity of gypsy moth populations, important task for forestry and agriculture. PMID:25052520

  17. Identification of a cis-acting DNA antisilencer element which modulates vimentin gene expression.

    PubMed Central

    Stover, D M; Zehner, Z E

    1992-01-01

    Vimentin is a tissue-specific, developmentally regulated member of the intermediate filament protein family normally expressed in cells of mesenchymal origin. Transcription factors which recognize specific cis-acting elements of the chicken gene include Sp-1 and the 95-kDa silencer protein which binds to a 40-bp silencer element at -608 (F. X. Farrell, C. M. Sax, and Z. E. Zehner, Mol. Cell. Biol. 10:2349-2358, 1990). In this study, we have identified a region upstream of the silencer element which restores gene activity. This region has been further delineated into two functional subelements of 75 and 260 bp. In transient transfection assays, the 75-bp element overrides the silencer effect of pStkCAT by 100%, while the 260-bp element is about half as active. Neither element affects gene activity when the silencer element is absent. Therefore, these elements do not function as enhancers, but they may serve only to override the silencer element and therefore can be viewed as antisilencers. In addition, the 75-bp element binds a specific 140-kDa protein, as determined by gel mobility shift assays and Southwestern (DNA-protein) blots, the binding site of which has been delineated to a 10- to 17-bp element by DNase I protection experiments. During myogenesis, a direct correlation can be made between the binding efficiency of the 140-kDa protein, the silencer protein, and gene activity in vivo. Genes known to contain a functional silencer element also contain at least one antisilencer element, as determined by sequence identity. Therefore, we have identified an antisilencer element and protein important in the developmental regulation of vimentin gene expression which may be involved in the regulation of other genes. Images PMID:1569950

  18. The Benzyl Moiety in a Quinoxaline-Based Scaffold Acts as a DNA Intercalation Switch.

    PubMed

    Mahata, Tridib; Kanungo, Ajay; Ganguly, Sudakshina; Modugula, Eswar Kalyan; Choudhury, Susobhan; Pal, Samir Kumar; Basu, Gautam; Dutta, Sanjay

    2016-06-27

    Quinoxaline antibiotics intercalate dsDNA and exhibit antitumor properties. However, they are difficult to synthesize and their structural complexity impedes a clear mechanistic understanding of DNA binding. Therefore design and synthesis of minimal-intercalators, using only part of the antibiotic scaffold so as to retain the key DNA-binding property, is extremely important. Reported is a unique example of a monomeric quinoxaline derivative of a 6-nitroquinoxaline-2,3-diamine scaffold which binds dsDNA by two different modes. While benzyl derivatives bound DNA in a sequential fashion, with intercalation as the second event, nonbenzyl derivatives showed only the first binding event. The benzyl intercalation switch provides important insights about molecular architecture which control specific DNA binding modes and would be useful in designing functionally important monomeric quinoxaline DNA binders and benchmarking molecular simulations.

  19. cis-acting components in the replication origin from ribosomal DNA of Saccharomyces cerevisiae.

    PubMed Central

    Miller, C A; Kowalski, D

    1993-01-01

    The ribosomal DNA (rDNA) repeats of Saccharomyces cerevisiae contain an autonomously replicating sequence (ARS) that colocalizes with a chromosomal origin of replication. We show that a minimal sequence necessary for full ARS function corresponds to a 107-bp rDNA fragment which contains three 10-of-11-bp matches to the ARS consensus sequence. Point mutations in only one of the 10-of-11-bp matches, GTTTAT GTTTT, inactivate the rDNA ARS, indicating that this consensus sequence is essential. A perfect match to a revised ARS consensus is present but not essential. Sequences up to 9 bp 5' from the essential consensus are dispensable. A broad DNA region directly 3' to the essential consensus is required and is easily unwound as indicated by: (i) hypersensitivity to nicking of an approximately 100-bp region by mung bean nuclease in a negatively supercoiled plasmid and (ii) helical instability determined by thermodynamic analysis of the nucleotide sequence. A correlation between DNA helical instability and replication efficiency of wild-type and mutated ribosomal ARS derivatives suggests that a broad region 3' to the essential ARS consensus functions as a DNA unwinding element. Certain point mutations that do not stabilize the DNA helix in the 3' region but reduce ARS efficiency reveal an element distinct from, but overlapping, the DNA unwinding element. The nucleotide sequence of the functionally important constituents in the ARS appears to be conserved among the rDNA repeats in the chromosome. Images PMID:8355687

  20. Shifts in rDNA levels act as a genome buffer promoting chromosome homeostasis.

    PubMed

    Deregowska, Anna; Adamczyk, Jagoda; Kwiatkowska, Aleksandra; Gurgul, Artur; Skoneczny, Marek; Skoneczna, Adrianna; Szmatola, Tomasz; Jasielczuk, Igor; Magda, Michal; Rawska, Ewa; Pabian, Sylwia; Panek, Anita; Kaplan, Jakub; Lewinska, Anna; Wnuk, Maciej

    2015-01-01

    The nucleolus is considered to be a stress sensor and rDNA-based regulation of cellular senescence and longevity has been proposed. However, the role of rDNA in the maintenance of genome integrity has not been investigated in detail. Using genomically diverse industrial yeasts as a model and array-based comparative genomic hybridization (aCGH), we show that chromosome level may be balanced during passages and as a response to alcohol stress that may be associated with changes in rDNA pools. Generation- and ethanol-mediated changes in genes responsible for protein and DNA/RNA metabolism were revealed using next-generation sequencing. Links between redox homeostasis, DNA stability, and telomere and nucleolus states were also established. These results suggest that yeast genome is dynamic and chromosome homeostasis may be controlled by rDNA. PMID:26566866

  1. 75 FR 60065 - Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ... Approval of an Information Collection; Lacey Act Declaration Requirement; Plants and Plant Products AGENCY... an information collection required by the Lacey Act for the importation of certain plants and plant...: For information on the Lacey Act declaration requirement, contact Mr. Craig Fedchock,...

  2. A rescue act: Translesion DNA synthesis past N(2) -deoxyguanosine adducts.

    PubMed

    Nair, Deepak T; Kottur, Jithesh; Sharma, Rahul

    2015-07-01

    Genomic DNA is continually subjected to a number of chemical insults that result in the formation of modified nucleotides--termed as DNA lesions. The N(2) -atom of deoxyguanosine is particularly reactive and a number of chemicals react at this site to form different kinds of DNA adducts. The N(2) -deoxyguanosine adducts perturb different genomic processes and are particularly deleterious for DNA replication as they have a strong tendency to inhibit replicative DNA polymerases. Many organisms possess specialized dPols--generally classified in the Y-family--that serves to rescue replication stalled at N(2) -dG and other adducts. A review of minor groove N(2) -adducts and the known strategies utilized by Y-family dPols to replicate past these lesions will be presented here.

  3. 76 FR 30835 - Requests for Modification or Revocation of Toxic Substances Control Act Section 5 Significant New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... action under docket identification (ID) number EPA-HQ-OPPT-2008-0296. All documents in the docket are... (202) 566-0280. Docket visitors are required to show photographic identification, pass through a metal... January 6, 2010 (75 FR 773) (FRL-8794-5), EPA had intended to include these modification requests. In...

  4. Maternal, Infant and Early Childhood Home Visiting Program in the Affordable Care Act: Supplemental Instruction Request-February 2011

    ERIC Educational Resources Information Center

    Schmit, Stephanie

    2011-01-01

    The latest Evidence-Based Home Visiting Supplemental Information Request (SIR) has recently been released by the Department of Health and Human Services (HHS) with collaboration from the Health Resources and Services Administration (HRSA) and the Administration for Children and Families (ACF) as outlined in the first Funding Opportunity…

  5. 78 FR 32390 - Food and Drug Administration Safety and Innovation Act (FDASIA): Request for Comments on the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-30

    ... Technology (ONC), and Federal Communication Commission (FCC) seek broad input from stakeholders and experts..., we are requesting comments on the topics identified in Section III. DATES: This Docket on regulations... Coordinator for Health Information Technology (ONC) and the Chairman of the Federal Communications...

  6. Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination

    PubMed Central

    Fredsøe, Jacob; Nielsen, Ida; Pedersen, Jakob Madsen; Bentsen, Iben Bach; Lisby, Michael; Bjergbaek, Lotte; Andersen, Anni H

    2015-01-01

    Faithful DNA replication with correct termination is essential for genome stability and transmission of genetic information. Here we have investigated the potential roles of Topoisomerase II (Top2) and the RecQ helicase Sgs1 during late stages of replication. We find that cells lacking Top2 and Sgs1 (or Top3) display two different characteristics during late S/G2 phase, checkpoint activation and accumulation of asymmetric X-structures, which are both independent of homologous recombination. Our data demonstrate that checkpoint activation is caused by a DNA structure formed at the strongest rDNA replication fork barrier (RFB) during replication termination, and consistently, checkpoint activation is dependent on the RFB binding protein, Fob1. In contrast, asymmetric X-structures are formed independent of Fob1 at less strong rDNA replication fork barriers. However, both checkpoint activation and formation of asymmetric X-structures are sensitive to conditions, which facilitate fork merging and progression of replication forks through replication fork barriers. Our data are consistent with a redundant role of Top2 and Sgs1 together with Top3 (Sgs1-Top3) in replication fork merging at rDNA barriers. At RFB either Top2 or Sgs1-Top3 is essential to prevent formation of a checkpoint activating DNA structure during termination, but at less strong rDNA barriers absence of the enzymes merely delays replication fork merging, causing an accumulation of asymmetric termination structures, which are solved over time. PMID:26630413

  7. Bacillus subtilis glutamine synthetase regulates its own synthesis by acting as a chaperone to stabilize GlnR-DNA complexes.

    PubMed

    Fisher, Susan H; Wray, Lewis V

    2008-01-22

    The Bacillus subtilis GlnR repressor controls gene expression in response to nitrogen availability. Because all GlnR-regulated genes are expressed constitutively in mutants lacking glutamine synthetase (GS), GS is required for repression by GlnR. Feedback-inhibited GS (FBI-GS) was shown to activate GlnR DNA binding with an in vitro electophoretic mobility shift assay (EMSA). The activation of GlnR DNA binding by GS in these experiments depended on the feedback inhibitor glutamine and did not occur with mutant GS proteins defective in regulating GlnR activity in vivo. Although stable GS-GlnR-DNA ternary complexes were not observed in the EMSA experiments, cross-linking experiments showed that a protein-protein interaction occurs between GlnR and FBI-GS. This interaction was reduced in the absence of the feedback inhibitor glutamine and with mutant GS proteins. Because FBI-GS significantly reduced the dissociation rate of the GlnR-DNA complexes, the stability of these complexes is enhanced by FBI-GS. These results argue that FBI-GS acts as a chaperone that activates GlnR DNA binding through a transient protein-protein interaction that stabilizes GlnR-DNA complexes. GS was shown to control the activity of the B. subtilis nitrogen transcription factor TnrA by forming a stable complex between FBI-GS and TnrA that inhibits TnrA DNA binding. Thus, B. subtilis GS is an enzyme with dual catalytic and regulatory functions that uses distinct mechanisms to control the activity of two different transcription factors.

  8. Antibacterial effects of Brazilian and Bulgarian propolis and synergistic effects with antibiotics acting on the bacterial DNA and folic acid.

    PubMed

    Orsi, R O; Fernandes, A; Bankova, V; Sforcin, J M

    2012-01-01

    Propolis is a honeybee product that has been used since ancient times because of its therapeutic effects. It can be used in the development of alternative therapies for the treatment of many diseases, and because propolis shows antibacterial action, this work was carried out in order to investigate a possible synergism between propolis and antibiotics acting on DNA (ciprofloxacin and norfloxacin) and on the metabolism (cotrimoxazole) against Salmonella typhi. Propolis samples collected in Brazil and Bulgaria were compared in these assays, and the synergism was investigated by using ½ and ¼ of the minimal inhibitory concentration for propolis and antibiotics, evaluating the number of viable cells according to the incubation time. Brazilian and Bulgarian propolis showed antibacterial activity, but no synergistic effects with the three tested antibiotics were seen. Previous works by our laboratory have revealed that propolis has synergistic effects with antibiotics, acting on the bacterial wall and ribosome, but it does not seem to interact with antibiotics acting on DNA or folic acid, and only a bacteriostatic action was seen in these assay conditions.

  9. 76 FR 52596 - Request for Comment Concerning Interpretations of the Magnuson-Moss Warranty Act; Rule Governing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... Commission published in the Federal Register (40 FR 60188) its Rule Governing Disclosure of Written Consumer... (40 FR 25721) providing interim guidance during the initial implementation of the Act. As the... the Federal Register (42 FR 36112) its Interpretations of the Magnuson-Moss Warranty Act to...

  10. Proteins bound at adjacent DNA elements act synergistically to regulate human proenkephalin cAMP inducible transcription.

    PubMed Central

    Comb, M; Mermod, N; Hyman, S E; Pearlberg, J; Ross, M E; Goodman, H M

    1988-01-01

    Synthesis of the endogenous opioid precursor, proenkephalin, is regulated by neurotransmitters and membrane depolarization. These events act through second messenger dependent signal transduction pathways via a short inducible DNA enhancer to regulate transcription of the proenkephalin gene. Two DNA elements located within this enhancer are essential for the transcriptional response to cAMP and phorbol ester. Inactivation of either element by mutation or by alteration of their stereospecific alignment eliminates inducible enhancer activity. The promoter distal element, ENKCRE-1, in the absence of a functional adjacent ENKCRE-2 element, has no inherent capacity to activate transcription. However, in the presence of a functional ENKCRE-2 element, this element synergistically augments cAMP and phorbol ester inducible transcription. The promoter proximal element, ENKCRE-2, is essential for both basal and regulated enhancer function. Four different protein factors found in HeLa cell nuclear extracts bind in vitro to the enhancer region. ENKTF-1, a novel enhancer binding protein, binds to the DNA region encompassing ENKCRE-1. The transcription factors AP-1 and AP-4 bind to overlapping sites spanning ENKCRE-2, and a fourth transcription factor, AP-2, binds to a site immediately downstream of ENKCRE-2. The binding of ENKTF-1 to mutant ENKCRE-1 sequences in vitro correlates with the in vivo inducibility of the mutant elements suggesting that ENKTF-1 acts in combination with factors that recognize the ENKCRE-2 domain to regulate cAMP inducible transcription. Together, the two DNA elements, ENKCRE-1 and ENKCRE-2 and the protein factors with which they interact, play a critical role in the transduction and reception of signals transmitted from cell surface receptors to the proenkephalin nuclear transcription complex. Images PMID:2850173

  11. A Werner syndrome protein homolog affects C. elegans development, growth rate, life span and sensitivity to DNA damage by acting at a DNA damage checkpoint.

    PubMed

    Lee, Se-Jin; Yook, Jong-Sung; Han, Sung Min; Koo, Hyeon-Sook

    2004-06-01

    A Werner syndrome protein homolog in C. elegans (WRN-1) was immunolocalized to the nuclei of germ cells, embryonic cells, and many other cells of larval and adult worms. When wrn-1 expression was inhibited by RNA interference (RNAi), a slight reduction in C. elegans life span was observed, with accompanying signs of premature aging, such as earlier accumulation of lipofuscin and tissue deterioration in the head. In addition, various developmental defects, including small, dumpy, ruptured, transparent body, growth arrest and bag of worms, were induced by RNAi. The frequency of these defects was accentuated by gamma-irradiation, implying that they were derived from spontaneous or induced DNA damage. wrn-1(RNAi) worms showed accelerated larval growth irrespective of gamma-irradiation, and pre-meiotic germ cells had an abnormal checkpoint response to DNA replication blockage. These observations suggest that WRN-1 acts as a checkpoint protein for DNA damage and replication blockage. This idea is also supported by an accelerated S phase in wrn-1(RNAi) embryonic cells. wrn-1(RNAi) phenotypes similar to those of Werner syndrome, such as premature aging and short stature, suggest wrn-1-deficient C. elegans as a useful model organism for Werner syndrome. PMID:15115755

  12. What Does It Take? California County Funding Requests for Recovery-Oriented Full Service Partnerships Under the Mental Health Services Act

    PubMed Central

    Cashin, Cheryl E.; Brown, Timothy T.

    2010-01-01

    The need to move mental health systems toward more recovery-oriented treatment modes is well established. Progress has been made to define needed changes but evidence is lacking about the resources required to implement them. The Mental Health Services Act (MHSA) in California was designed to implement more recovery-oriented treatment modes. We use data from county funding requests and annual updates to examine how counties budgeted for recovery-oriented programs targeted to different age groups under MHSA. Findings indicate that initial per-client budgeting for Full Services Partnerships under MHSA was maintained in future cycles and counties budgeted less per client for children. With this analysis, we begin to benchmark resource allocation for programs that are intended to be recovery-oriented, which should be evaluated against appropriate outcome measures in the future to determine the degree of recovery-orientation. PMID:20440560

  13. A conserved domain of the large subunit of replication factor C binds PCNA and acts like a dominant negative inhibitor of DNA replication in mammalian cells.

    PubMed

    Fotedar, R; Mossi, R; Fitzgerald, P; Rousselle, T; Maga, G; Brickner, H; Messier, H; Kasibhatla, S; Hübscher, U; Fotedar, A

    1996-08-15

    Replication factor C (RF-C), a complex of five polypeptides, is essential for cell-free SV40 origin-dependent DNA replication and viability in yeast. The cDNA encoding the large subunit of human RF-C (RF-Cp145) was cloned in a Southwestern screen. Using deletion mutants of RF-Cp145 we have mapped the DNA binding domain of RF-Cp145 to amino acid residues 369-480. This domain is conserved among both prokaryotic DNA ligases and eukaryotic poly(ADP-ribose) polymerases and is absent in other subunits of RF-C. The PCNA binding domain maps to amino acid residues 481-728 and is conserved in all five subunits of RF-C. The PCNA binding domain of RF-Cp145 inhibits several functions of RF-C, such as: (i) in vitro DNA replication of SV40 origin-containing DNA; (ii) RF-C-dependent loading of PCNA onto DNA; and (iii) RF-C-dependent DNA elongation. The PCNA binding domain of RF-Cp145 localizes to the nucleus and inhibits DNA synthesis in transfected mammalian cells. In contrast, the DNA binding domain of RF-Cp145 does not inhibit DNA synthesis in vitro or in vivo. We therefore conclude that amino acid residues 481-728 of human RF-Cp145 are critical and act as a dominant negative mutant of RF-C function in DNA replication in vivo.

  14. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE INDIAN COUNTRY: AIR QUALITY PLANNING AND... Indian tribe's authority to regulate air quality. For applications covering areas within the exterior... Self-Determination and Education Assistance Act (25 U.S.C. 450, et seq.), the Indian...

  15. 75 FR 13241 - Request for Comment on Implementation of the Family Smoking Prevention and Tobacco Control Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... its provisions to the regulation promulgated by FDA in 1996 (61 FR 44396, August 28, 1996) (1996 final... Implementation of the Family Smoking Prevention and Tobacco Control Act AGENCY: Food and Drug Administration, HHS... advertising of cigarettes and smokeless tobacco. Elsewhere in this issue of the Federal Register, FDA...

  16. 78 FR 26374 - An Evaluation of the Prescription Drug User Fee Act Workload Adjuster; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-06

    ...,'' subsection B, which was reauthorized by the Food and Drug Administration Safety and Innovation Act (FDASIA... changes in workload volume and complexity in the human drug review program and present options to... public comment, FDA can adopt appropriate change to the workload adjustment methodology, if...

  17. A saturation screen for cis-acting regulatory DNA in the Hox genes of Ciona intestinalis

    SciTech Connect

    Keys, David N.; Lee, Byung-in; Di Gregorio, Anna; Harafuji, Naoe; Detter, Chris; Wang, Mei; Kahsai, Orsalem; Ahn, Sylvia; Arellano, Andre; Zhang, Quin; Trong, Stephan; Doyle, Sharon A.; Satoh, Noriyuki; Satou, Yutaka; Saiga, Hidetoshi; Christian, Allen; Rokhsar, Dan; Hawkins, Trevor L.; Levine, Mike; Richardson, Paul

    2005-01-05

    A screen for the systematic identification of cis-regulatory elements within large (>100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis. Randomly generated DNA fragments from bacterial artificial chromosomes containing two clusters of Hox genes were inserted into a vector upstream of a minimal promoter and lacZ reporter gene. A total of 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulatory activities were monitored in larvae. In sum, 21 separable cis-regulatory elements were found. These include eight Hox linked domains that drive expression in nested anterior-posterior domains of ectodermally derived tissues. In addition to vertebrate-like CNS regulation, the discovery of cis-regulatory domains that drive epidermal transcription suggests that C. intestinalis has arthropod-like Hox patterning in the epidermis.

  18. Cis-acting elements in the lytic origin of DNA replication of Marek's disease virus type 1.

    PubMed

    Katsumata, A; Iwata, A; Ueda, S

    1998-12-01

    The replication origin of Marek's disease virus (MDV) type 1 was analysed by using a transient replication assay with plasmids containing various fragments of MDV strain Md5 genomic DNA. Plasmid pMBH, containing the BamHI-H fragment, showed replication activity in MDV-infected chicken embryonic fibroblasts (CEF). By deletion analysis of pMBH, two regions, the promoter-enhancer region of the MDV pp38 gene and the 132 bp tandem direct repeat, were shown to be required for replication activity. Replication of pMBH was not observed in uninfected CEF, suggesting that a trans-acting factor(s) encoded by the MDV genome was necessary for replication.

  19. Immunomodulatory drugs act as inhibitors of DNA methyltransferases and induce PU.1 up-regulation in myeloma cells.

    PubMed

    Endo, Shinya; Amano, Masayuki; Nishimura, Nao; Ueno, Niina; Ueno, Shikiko; Yuki, Hiromichi; Fujiwara, Shiho; Wada, Naoko; Hirata, Shinya; Hata, Hiroyuki; Mitsuya, Hiroaki; Okuno, Yutaka

    2016-01-01

    Immunomodulatory drugs (IMiDs) such as thalidomide, lenalidomide, and pomalidomide are efficacious in the treatment of multiple myeloma and significantly prolong their survival. However, the mechanisms of such effects of IMiDs have not been fully elucidated. Recently, cereblon has been identified as a target binding protein of thalidomide. Lenalidomide-resistant myeloma cell lines often lose the expression of cereblon, suggesting that IMiDs act as an anti-myeloma agent through interacting with cereblon. Cereblon binds to damaged DNA-binding protein and functions as a ubiquitin ligase, inducing degradation of IKZF1 and IKZF3 that are essential transcription factors for B and T cell development. Degradation of both IKZF1 and IKZF3 reportedly suppresses myeloma cell growth. Here, we found that IMiDs act as inhibitors of DNA methyltransferases (DMNTs). We previously reported that PU.1, which is an ETS family transcription factor and essential for myeloid and lymphoid development, functions as a tumor suppressor in myeloma cells. PU.1 induces growth arrest and apoptosis of myeloma cell lines. In this study, we found that low-dose lenalidomide and pomalidomide up-regulate PU.1 expression through inducing demethylation of the PU.1 promoter. In addition, IMiDs inhibited DNMT1, DNMT3a, and DNMT3b activities in vitro. Furthermore, lenalidomide and pomalidomide decreased the methylation status of the whole genome in myeloma cells. Collectively, IMiDs exert demethylation activity through inhibiting DNMT1, 3a, and 3b, and up-regulating PU.1 expression, which may be one of the mechanisms of the anti-myeloma activity of IMiDs.

  20. Methyl-CpG binding domain protein acts to regulate the repair of cyclobutane pyrimidine dimers on rice DNA

    PubMed Central

    Fang, Changxun; Chen, Weisi; Li, Chengxun; Jian, Xin; Li, Yingzhe; Lin, Hongmei; Lin, Wenxiong

    2016-01-01

    UVB radiation causes cyclobutane pyrimidine dimers (CPDs) to form on the DNA of living organisms. This study found that overexpression of the silicon absorbance gene Lsi1 reduced the accumulation of CPDs in rice, which profited from the reactivation by photolyase. The transcript abundance of deoxyribodipyrimidine photolyase (Os10g0167600) was generally correlated with the silicon content of the rice, and the up-regulation of Os10g0167600 was found to be highest in the UVB-treated Lsi1-overexpressed (Lsi1-OX) rice. A trans-acting factor, methyl-CpG binding domain protein (OsMeCP), was found to interact with the cis-element of Os10g0167600. The nucleic location of OsMeCP effectively enabled the transcriptional regulation. Compared with the WT, the level of OsMeCP was lower in the Lsi1-OX rice but higher in the Lsi1-RNAi line. Rice cultured in a high silicate-concentration solution also exhibited less OsMeCP abundance. Overexpression of OsMeCP led to lower Os10g0167600 transcript levels and a higher CPD content than in the WT, but the reverse was true in the OsMeCP-RNAi line. These findings indicate that OsMeCP acts as a negative regulator of silicon, and can mediate the repression of the transcription from Os10g0167600, which inhibits the photoreactivation of the photolyase involved in the repair of CPDs. PMID:27694845

  1. Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome

    PubMed Central

    Riber, Leise; Olsson, Jan A.; Jensen, Rasmus B.; Skovgaard, Ole; Dasgupta, Santanu; Marinus, Martin G.; Løbner-Olesen, Anders

    2006-01-01

    Initiation of DNA replication in Eschericia coli requires the ATP-bound form of the DnaA protein. The conversion of DnaA–ATP to DnaA–ADP is facilitated by a complex of DnaA, Hda (homologous to DnaA), and DNA-loaded β-clamp proteins in a process termed RIDA (regulatory inactivation of DnaA). Hda-deficient cells initiate replication at each origin mainly once per cell cycle, and the rare reinitiation events never coincide with the end of the origin sequestration period. Therefore, RIDA is not the predominant mechanism to prevent immediate reinitiation from oriC. The cellular level of Hda correlated directly with dnaA gene expression such that Hda deficiency led to reduced dnaA gene expression, and overproduction of Hda led to DnaA overproduction. Hda-deficient cells were very sensitive to variations in the cellular level of DnaA, and DnaA overproduction led to uncontrolled initiation of replication from oriC, causing severe growth retardation or cell death. Based on these observations, we propose that both RIDA and dnaA gene autoregulation are required as homeostatic mechanisms to ensure that initiation of replication occurs at the same time relative to cell mass in each cell cycle. PMID:16882985

  2. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2010-04-01 2010-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  3. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2014-04-01 2014-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  4. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2013-04-01 2013-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  5. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2012-04-01 2012-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  6. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2011-04-01 2011-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  7. A negatively acting DNA sequence element mediates phytochrome-directed repression of phyA gene transcription.

    PubMed Central

    Bruce, W B; Deng, X W; Quail, P H

    1991-01-01

    Phytochrome represses transcription of its own phyA genes within 5 min of light-triggered conversion to its active Pfr form. We have utilized microprojectile mediated gene transfer into etiolated rice seedlings to delineate sequence elements in the oat phyA3 promoter responsible for this regulation. Linker-scan mutagenesis of this promoter has identified two positive elements which together are necessary for maximal transcription in the absence of Pfr. These elements are designated PE1, centered at position -357 bp, and PE3, centered at position -96 bp. Sequence mutagenesis immediately downstream of PE3 results in maximal transcription in the presence of high Pfr levels, indicating that Pfr represses phyA3 transcription through a negatively acting sequence element. This element, designated RE1, with the sequence CATGGGCGCGG, encompasses a motif that is highly conserved in all monocot phyA promoters thus far characterized. DNase I protection analysis indicates that oat nuclear extracts contain multiple factors that bind to an array of sequence motifs, including PE1 and part of PE3, within 400 bp upstream of the oat phyA3 transcription start site. This DNA-binding pattern is not altered by Pfr. Weak binding to part of the RE1 motif is evident but also with no difference between high and low Pfr levels. We conclude that the signal transduction chain that mediates Pfr-directed repression of phyA3 transcription terminates with a negatively acting transcription factor that binds to the sequence element RE1. Images PMID:1915276

  8. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  9. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  10. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  11. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  12. DNA.

    ERIC Educational Resources Information Center

    Felsenfeld, Gary

    1985-01-01

    Structural form, bonding scheme, and chromatin structure of and gene-modification experiments with deoxyribonucleic acid (DNA) are described. Indicates that DNA's double helix is variable and also flexible as it interacts with regulatory and other molecules to transfer hereditary messages. (DH)

  13. 32 CFR 701.46 - Aggregating requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... that are for a commercial use, a DON activity may not charge for the first 2 hours of search time or.... When an activity reasonably believes that a requester or, on rare occasions, a group of requesters acting in concert, is attempting to break a request down into a series of requests for the purpose...

  14. 32 CFR 701.46 - Aggregating requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... that are for a commercial use, a DON activity may not charge for the first 2 hours of search time or.... When an activity reasonably believes that a requester or, on rare occasions, a group of requesters acting in concert, is attempting to break a request down into a series of requests for the purpose...

  15. 7 CFR 1.6 - Aggregating requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Aggregating requests. 1.6 Section 1.6 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Official Records § 1.6 Aggregating requests. When an agency reasonably believes that a requester, or a group of requesters acting in...

  16. 7 CFR 1.6 - Aggregating requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Aggregating requests. 1.6 Section 1.6 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Official Records § 1.6 Aggregating requests. When an agency reasonably believes that a requester, or a group of requesters acting in...

  17. 14 CFR 1212.300 - Requesting amendment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....300 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Amendments to Privacy Act Records § 1212.300 Requesting amendment. Individuals may request that NASA amend their records maintained in a NASA system of records. This request shall be in writing, addressed to...

  18. 14 CFR 1212.300 - Requesting amendment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ....300 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Amendments to Privacy Act Records § 1212.300 Requesting amendment. Individuals may request that NASA amend their records maintained in a NASA system of records. This request shall be in writing, addressed to...

  19. 14 CFR 1212.300 - Requesting amendment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ....300 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Amendments to Privacy Act Records § 1212.300 Requesting amendment. Individuals may request that NASA amend their records maintained in a NASA system of records. This request shall be in writing, addressed to...

  20. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  1. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  2. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  3. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  4. 36 CFR 1008.13 - Requests for access to records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Requests for access to records. 1008.13 Section 1008.13 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.13 Requests for access to records. The Privacy Act permits individuals, upon request,...

  5. DNA

    ERIC Educational Resources Information Center

    Stent, Gunther S.

    1970-01-01

    This history for molecular genetics and its explanation of DNA begins with an analysis of the Golden Jubilee essay papers, 1955. The paper ends stating that the higher nervous system is the one major frontier of biological inquiry which still offers some romance of research. (Author/VW)

  6. 45 CFR 1171.5 - Requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... INFORMATION ACT § 1171.5 Requests for records. (a) How to make a request. Your FOIA request need not be in any... request. (b) Agreement to pay fees. If you make a FOIA request, the NEH will consider it an agreement by... Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND...

  7. 22 CFR 503.2 - Making a request.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Making a request. 503.2 Section 503.2 Foreign Relations BROADCASTING BOARD OF GOVERNORS FREEDOM OF INFORMATION ACT REGULATION § 503.2 Making a request. (a) How to request records. All requests for documents shall be made in writing. Requests should...

  8. 22 CFR 503.2 - Making a request.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 2 2013-04-01 2009-04-01 true Making a request. 503.2 Section 503.2 Foreign Relations BROADCASTING BOARD OF GOVERNORS FREEDOM OF INFORMATION ACT REGULATION § 503.2 Making a request. (a) How to request records. All requests for documents shall be made in writing. Requests should...

  9. 22 CFR 503.2 - Making a request.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 2 2014-04-01 2014-04-01 false Making a request. 503.2 Section 503.2 Foreign Relations BROADCASTING BOARD OF GOVERNORS FREEDOM OF INFORMATION ACT REGULATION § 503.2 Making a request. (a) How to request records. All requests for documents shall be made in writing. Requests should...

  10. 22 CFR 503.2 - Making a request.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 2 2012-04-01 2009-04-01 true Making a request. 503.2 Section 503.2 Foreign Relations BROADCASTING BOARD OF GOVERNORS FREEDOM OF INFORMATION ACT REGULATION § 503.2 Making a request. (a) How to request records. All requests for documents shall be made in writing. Requests should...

  11. 22 CFR 503.2 - Making a request.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Making a request. 503.2 Section 503.2 Foreign Relations BROADCASTING BOARD OF GOVERNORS FREEDOM OF INFORMATION ACT REGULATION § 503.2 Making a request. (a) How to request records. All requests for documents shall be made in writing. Requests should...

  12. 76 FR 78662 - Proposed Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    ...; Comment Request Proposed Projects: Title: Plan for Foster Care and Adoption Assistance--Title IV-E. OMB No... Act (the Act) for each public child welfare agency requesting Federal funding for foster...

  13. 77 FR 38637 - Proposed Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-28

    ...; Comment Request Proposed Projects Title: Plan for Foster Care and Adoption Assistance--Title IV-E. OMB No... Act (the Act) for each public child welfare agency requesting Federal funding for foster...

  14. The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.

    PubMed

    Costes, Audrey; Lecointe, François; McGovern, Stephen; Quevillon-Cheruel, Sophie; Polard, Patrice

    2010-01-01

    We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter)) as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter) interactome using GFP fusions and by Tap-tag and biochemical analysis. It includes at least 12 proteins. The interactome was previously shown to include PriA, RecG, and RecQ and extended in this study by addition of DnaE, SbcC, RarA, RecJ, RecO, XseA, Ung, YpbB, and YrrC. Targeting of YpbB to active forks appears to depend on RecS, a RecQ paralogue, with which it forms a stable complex. Most of these SSB partners are conserved in bacteria, while others, such as the essential DNA polymerase DnaE, YrrC, and the YpbB/RecS complex, appear to be specific to B. subtilis. SSB(Cter) deletion has a moderate impact on B. subtilis cell growth. However, it markedly affects the efficiency of repair of damaged genomic DNA and arrested replication forks. ssbΔCter mutant cells appear deficient in RecA loading on ssDNA, explaining their inefficiency in triggering the SOS response upon exposure to genotoxic agents. Together, our findings show that the bacterial SSB(Cter) acts as a DNA maintenance hub at active chromosomal forks that secures their propagation along the genome. PMID:21170359

  15. Efficacy of an amphipathic oligopeptide to shuttle and release a cis-acting DNA decoy into human cells.

    PubMed

    Citti, L; Rovero, P; Colombo, M G; Mariani, L; Poliseno, L; Rainaldi, G

    2002-01-01

    We investigated the ability of an amphipathic oligopeptide to carry a synthetic dsDNA oligonucleotide inside human cells. The oligonucleotide was designed as a decoy binding site for the transcriptional activator of the methylguanine-DNA methyltransferase (MGMT) gene. The complex oligopeptide and decoy were administered to MCF10A exponentially growing cells, and the uptake was monitored by flow cytometry. After a 1-h exposure, almost all of the MCF10A cells were fluorescent, indicating that all of the cells had been transfected. By increasing the time, the fluorescence intensity per cell rapidly increased to a plateau at the 8-h time point. RT-PCR analysis of the MGMT gene was used as the molecular readout of the intracellular activity of the DNA decoy. MCF10A cells transfected with the oligopeptide/decoy complex showed a strong reduction in MGMT mRNA. Here, we discuss the advantages of using amphipathic oligopeptides as carriers of short DNA sequences.

  16. 20 CFR 401.35 - Your right to request records.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Act (see 20 CFR part 402) allows you to request information from SSA whether or not it is in a system... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Your right to request records. 401.35 Section... AND INFORMATION The Privacy Act § 401.35 Your right to request records. The Privacy Act gives you...

  17. 77 FR 5481 - Notice of Request for Extension of Approval of an Information Collection; Special Need Requests...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ... Collection; Special Need Requests Under the Plant Protection Act AGENCY: Animal and Plant Health Inspection... INFORMATION CONTACT: For information on special need requests under the Plant Protection Act, contact Ms. Lynn...: Title: Special Need Request Under the Plant Protection Act. OMB Number: 0579-0291. Type of...

  18. cis-Acting sequences in addition to donor and acceptor sites are required for template switching during synthesis of plus-strand DNA for duck hepatitis B virus.

    PubMed Central

    Havert, M B; Loeb, D D

    1997-01-01

    A characteristic of all hepadnaviruses is the relaxed-circular conformation of the DNA genome within an infectious virion. Synthesis of the relaxed-circular genome by reverse transcription requires three template switches. These template switches, as for the template switches or strand transfers of other reverse-transcribing genetic elements, require repeated sequences (the donor and acceptor sites) between which a complementary strand of nucleic acid is transferred. The mechanism for each of the template switches in hepadnaviruses is poorly understood. To determine whether sequences other than the donor and acceptor sites are involved in the template switches of duck hepatitis B virus (DHBV), a series of molecular clones which express viral genomes bearing deletion mutations were analyzed. We found that three regions of the DHBV genome, which are distinct from the donor and acceptor sites, are required for the synthesis of relaxed-circular DNA. One region, located near the 3' end of the minus-strand template, is required for the template switch that circularizes the genome. The other two regions, located in the middle of the genome and near DR2, appear to be required for plus-strand primer translocation. We speculate that these cis-acting sequences may play a role in the organization of the minus-strand DNA template within the capsid particle so that it supports efficient template switching during plus-strand DNA synthesis. PMID:9188603

  19. 76 FR 70111 - Proposed Information Collection; Comment Request; Request for Investigation Under Section 232 of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Bureau of Industry and Security Proposed Information Collection; Comment Request; Request for Investigation Under Section 232 of the Trade Expansion Act AGENCY: Bureau of Industry and Security,...

  20. XPF-ERCC1 acts in unhooking DNA interstrand crosslinks in cooperation with FANCD2 and FANCP/SLX4

    PubMed Central

    Douwel, Daisy Klein; Boonen, Rick A.C.M.; Long, David T.; Szypowska, Anna A.; Räschle, Markus; Walter, Johannes C.; Knipscheer, Puck

    2016-01-01

    Summary DNA interstrand crosslinks (ICLs), highly toxic lesions that covalently link the Watson and Crick strands of the double helix, are repaired by a complex, replication-coupled pathway in higher eukaryotes. The earliest DNA processing event in ICL repair is the incision of parental DNA on either side of the ICL (“unhooking”), which allows lesion bypass. Incisions depend critically on the Fanconi anemia pathway, whose activation involves ubiquitylation of the FANCD2 protein. Using Xenopus egg extracts, which support replication-coupled ICL repair, we show that the 3′ flap endonuclease XPF-ERCC1 cooperates with SLX4/FANCP to carry out the unhooking incisions. Efficient recruitment of XPF-ERCC1 and SLX4 to the ICL depends on FANCD2 and its ubiquitylation. These data help define the molecular mechanism by which the Fanconi anemia pathway promotes a key event in replication-coupled ICL repair. PMID:24726325

  1. Embryonic caffeine exposure acts via A1 adenosine receptors to alter adult cardiac function and DNA methylation in mice.

    PubMed

    Buscariollo, Daniela L; Fang, Xiefan; Greenwood, Victoria; Xue, Huiling; Rivkees, Scott A; Wendler, Christopher C

    2014-01-01

    Evidence indicates that disruption of normal prenatal development influences an individual's risk of developing obesity and cardiovascular disease as an adult. Thus, understanding how in utero exposure to chemical agents leads to increased susceptibility to adult diseases is a critical health related issue. Our aim was to determine whether adenosine A1 receptors (A1ARs) mediate the long-term effects of in utero caffeine exposure on cardiac function and whether these long-term effects are the result of changes in DNA methylation patterns in adult hearts. Pregnant A1AR knockout mice were treated with caffeine (20 mg/kg) or vehicle (0.09% NaCl) i.p. at embryonic day 8.5. This caffeine treatment results in serum levels equivalent to the consumption of 2-4 cups of coffee in humans. After dams gave birth, offspring were examined at 8-10 weeks of age. A1AR+/+ offspring treated in utero with caffeine were 10% heavier than vehicle controls. Using echocardiography, we observed altered cardiac function and morphology in adult mice exposed to caffeine in utero. Caffeine treatment decreased cardiac output by 11% and increased left ventricular wall thickness by 29% during diastole. Using DNA methylation arrays, we identified altered DNA methylation patterns in A1AR+/+ caffeine treated hearts, including 7719 differentially methylated regions (DMRs) within the genome and an overall decrease in DNA methylation of 26%. Analysis of genes associated with DMRs revealed that many are associated with cardiac hypertrophy. These data demonstrate that A1ARs mediate in utero caffeine effects on cardiac function and growth and that caffeine exposure leads to changes in DNA methylation.

  2. RecO acts with RecF and RecR to protect and maintain replication forks blocked by UV-induced DNA damage in Escherichia coli.

    PubMed

    Chow, Kin-Hoe; Courcelle, Justin

    2004-01-30

    In Escherichia coli, recF and recR are required to stabilize and maintain replication forks arrested by UV-induced DNA damage. In the absence of RecF, replication fails to recover, and the nascent lagging strand of the arrested replication fork is extensively degraded by the RecQ helicase and RecJ nuclease. recO mutants are epistatic with recF and recR with respect to recombination and survival assays after DNA damage. In this study, we show that RecO functions with RecF and RecR to protect the nascent lagging strand of arrested replication forks after UV-irradiation. In the absence of RecO, the nascent DNA at arrested replication forks is extensively degraded and replication fails to recover. The extent of nascent DNA degradation is equivalent in single, double, or triple mutants of recF, recO, or recR, and the degradation is dependent upon RecJ and RecQ functions. Because RecF has been shown to protect the nascent lagging strand from degradation, these observations indicate that RecR and RecO function with RecF to protect the same nascent strand of the arrested replication fork and are likely to act at a common point during the recovery process. We discuss these results in relation to the biochemical and cellular properties of RecF, RecO, and RecR and their potential role in loading RecA filaments to maintain the replication fork structure after the arrest of replication by UV-induced DNA damage. PMID:14625283

  3. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. 49.5... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as... for which it would be inappropriate to treat tribes in general in the same manner as States....

  4. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  5. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  6. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  7. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  8. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  9. The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers.

    PubMed

    Zech, Juergen; Godfrey, Emma Louise; Masai, Hisao; Hartsuiker, Edgar; Dalgaard, Jacob Zeuthen

    2015-01-01

    During S-phase replication forks can stall at specific genetic loci. At some loci, the stalling events depend on the replisome components Schizosaccharomyces pombe Swi1 (Saccharomyces cerevisiae Tof1) and Swi3 (S. cerevisiae Csm3) as well as factors that bind DNA in a site-specific manner. Using a new genetic screen we identified Mrc1 (S. cerevisiae Mrc1/metazoan Claspin) as a replisome component involved in replication stalling. Mrc1 is known to form a sub-complex with Swi1 and Swi3 within the replisome and is required for the intra-S phase checkpoint activation. This discovery is surprising as several studies show that S. cerevisiae Mrc1 is not required for replication barrier activity. In contrast, we show that deletion of S. pombe mrc1 leads to an approximately three-fold reduction in barrier activity at several barriers and that Mrc1's role in replication fork stalling is independent of its role in checkpoint activation. Instead, S. pombe Mrc1 mediated fork stalling requires the presence of a functional copy of its phylogenetically conserved DNA binding domain. Interestingly, this domain is on the sequence level absent from S. cerevisiae Mrc1. Our study indicates that direct interactions between the eukaryotic replisome and the DNA are important for site-specific replication stalling.

  10. Cadmium treatment suppresses DNA polymerase δ catalytic subunit gene expression by acting on the p53 and Sp1 regulatory axis.

    PubMed

    Antoniali, Giulia; Marcuzzi, Federica; Casarano, Elena; Tell, Gianluca

    2015-11-01

    Cadmium (Cd) is a carcinogenic and neurotoxic environmental pollutant. Among the proposed mechanisms for Cd toxic effects, its ability to promote oxidative stress and to inhibit, in vitro, the activities of some Base Excision DNA Repair (BER) enzymes, such as hOGG1, XRCC1 and APE1, have been already established. However, the molecular mechanisms at the basis of these processes are largely unknown especially at sub-lethal doses of Cd and no information is available on the effect of Cd on the expression levels of BER enzymes. Here, we show that non-toxic treatment of neuronal cell lines, with pro-mitogenic doses of Cd, promotes a significant time- and dose-dependent down-regulation of DNA polymerase δ (POLD1) expression through a transcriptional mechanism with a modest effect on Polβ, XRCC1 and APE1. We further elucidated that the observed transcriptional repression on Polδ is acted by through competition by activated p53 on Sp1 at POLD1 promoter and by a squelching effect. We further proved the positive effect of Sp1 not only on POLD1 expression but also on Polβ, XRCC1 and APE1 expression, suggesting that Sp1 has pleiotropic effects on the whole BER pathway. Our results indicated that Cd-mediated impairment of BER pathway, besides acting on the enzymatic functions of some key proteins, is also exerted at the gene expression level of Polδ by acting on the p53-Sp1 regulatory axis. These data may explain not only the Cd-induced neurotoxic effects but also the potential carcinogenicity of this heavy metal.

  11. Polymorphic tandem repeats within gene promoters act as modifiers of gene expression and DNA methylation in humans

    PubMed Central

    Quilez, Javier; Guilmatre, Audrey; Garg, Paras; Highnam, Gareth; Gymrek, Melissa; Erlich, Yaniv; Joshi, Ricky S.; Mittelman, David; Sharp, Andrew J.

    2016-01-01

    Despite representing an important source of genetic variation, tandem repeats (TRs) remain poorly studied due to technical difficulties. We hypothesized that TRs can operate as expression (eQTLs) and methylation (mQTLs) quantitative trait loci. To test this we analyzed the effect of variation at 4849 promoter-associated TRs, genotyped in 120 individuals, on neighboring gene expression and DNA methylation. Polymorphic promoter TRs were associated with increased variance in local gene expression and DNA methylation, suggesting functional consequences related to TR variation. We identified >100 TRs associated with expression/methylation levels of adjacent genes. These potential eQTL/mQTL TRs were enriched for overlaps with transcription factor binding and DNaseI hypersensitivity sites, providing a rationale for their effects. Moreover, we showed that most TR variants are poorly tagged by nearby single nucleotide polymorphisms (SNPs) markers, indicating that many functional TR variants are not effectively assayed by SNP-based approaches. Our study assigns biological significance to TR variations in the human genome, and suggests that a significant fraction of TR variations exert functional effects via alterations of local gene expression or epigenetics. We conclude that targeted studies that focus on genotyping TR variants are required to fully ascertain functional variation in the genome. PMID:27060133

  12. Polymorphic tandem repeats within gene promoters act as modifiers of gene expression and DNA methylation in humans.

    PubMed

    Quilez, Javier; Guilmatre, Audrey; Garg, Paras; Highnam, Gareth; Gymrek, Melissa; Erlich, Yaniv; Joshi, Ricky S; Mittelman, David; Sharp, Andrew J

    2016-05-01

    Despite representing an important source of genetic variation, tandem repeats (TRs) remain poorly studied due to technical difficulties. We hypothesized that TRs can operate as expression (eQTLs) and methylation (mQTLs) quantitative trait loci. To test this we analyzed the effect of variation at 4849 promoter-associated TRs, genotyped in 120 individuals, on neighboring gene expression and DNA methylation. Polymorphic promoter TRs were associated with increased variance in local gene expression and DNA methylation, suggesting functional consequences related to TR variation. We identified >100 TRs associated with expression/methylation levels of adjacent genes. These potential eQTL/mQTL TRs were enriched for overlaps with transcription factor binding and DNaseI hypersensitivity sites, providing a rationale for their effects. Moreover, we showed that most TR variants are poorly tagged by nearby single nucleotide polymorphisms (SNPs) markers, indicating that many functional TR variants are not effectively assayed by SNP-based approaches. Our study assigns biological significance to TR variations in the human genome, and suggests that a significant fraction of TR variations exert functional effects via alterations of local gene expression or epigenetics. We conclude that targeted studies that focus on genotyping TR variants are required to fully ascertain functional variation in the genome. PMID:27060133

  13. Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity

    PubMed Central

    Tan, Benedict G.; Wellesley, Frederick C.; Savery, Nigel J.; Szczelkun, Mark D.

    2016-01-01

    The guanine (G)-tract of conserved sequence block 2 (CSB 2) in human mitochondrial DNA can result in transcription termination due to formation of a hybrid G-quadruplex between the nascent RNA and the nontemplate DNA strand. This structure can then influence genome replication, stability and localization. Here we surveyed the frequency of variation in sequence identity and length at CSB 2 amongst human mitochondrial genomes and used in vitro transcription to assess the effects of this length heterogeneity on the activity of the mitochondrial RNA polymerase, POLRMT. In general, increased G-tract length correlated with increased termination levels. However, variation in the population favoured CSB 2 sequences which produced efficient termination while particularly weak or strong signals were avoided. For all variants examined, the 3′ end of the transcripts mapped to the same downstream sequences and were prevented from terminating by addition of the transcription factor TEFM. We propose that CSB 2 length heterogeneity allows variation in the efficiency of transcription termination without affecting the position of the products or the capacity for regulation by TEFM. PMID:27436287

  14. 36 CFR 1120.25 - Form of requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Form of requests. 1120.25... BOARD PUBLIC AVAILABILITY OF INFORMATION Information Available Upon Request § 1120.25 Form of requests... otherwise need not be in any particular form. Each request under the Freedom of Information Act should...

  15. 75 FR 77663 - Information Collection Extension Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-13

    ... of the Secretary Information Collection Extension Request ACTION: Notice. SUMMARY: The Department of... comment on information collection extension request in accordance with the Paperwork Reduction Act of 1995... Information Report--29 CFR part 31 (Title VI of the Civil Rights Act), Nondiscrimination--Disability--29...

  16. 14 CFR 1212.300 - Requesting amendment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ....300 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Amendments to Privacy Act Records § 1212.300 Requesting amendment. Individuals may request that NASA amend their records maintained in a NASA system of records under the provisions of 5 U.S.C. 552a(d)(2)....

  17. Identification of two telomere-proximal fission yeast DNA replication origins constrained by nearby cis-acting sequences to replicate in late S phase

    PubMed Central

    Chaudari, Amna; Huberman, Joel A

    2012-01-01

    Telomeres of the fission yeast,  Schizosaccharomyces pombe, are known to replicate in late S phase, but the reasons for this late replication are not fully understood. We have identified two closely-spaced DNA replication origins, 5.5 to 8 kb upstream from the telomere itself. These are the most telomere-proximal of all the replication origins in the fission yeast genome. When located by themselves in circular plasmids, these origins fired in early S phase, but if flanking sequences closer to the telomere were included in the circular plasmid, then replication was restrained to late S phase – except in cells lacking the replication-checkpoint kinase, Cds1. We conclude that checkpoint-dependent late replication of telomere-associated sequences is dependent on nearby cis-acting sequences, not on proximity to the physical end of a linear chromosome. PMID:24358832

  18. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  19. 45 CFR 612.3 - Requirements for making requests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... how to make a Privacy Act request (see NSF Privacy Act regulations at 45 CFR 613.2). (2) If you are... 45 Public Welfare 3 2011-10-01 2011-10-01 false Requirements for making requests. 612.3 Section... AVAILABILITY OF RECORDS AND INFORMATION § 612.3 Requirements for making requests. (a) Where to send a...

  20. 45 CFR 612.3 - Requirements for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... how to make a Privacy Act request (see NSF Privacy Act regulations at 45 CFR 613.2). (2) If you are... 45 Public Welfare 3 2010-10-01 2010-10-01 false Requirements for making requests. 612.3 Section... AVAILABILITY OF RECORDS AND INFORMATION § 612.3 Requirements for making requests. (a) Where to send a...

  1. 45 CFR 612.3 - Requirements for making requests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... how to make a Privacy Act request (see NSF Privacy Act regulations at 45 CFR 613.2). (2) If you are... 45 Public Welfare 3 2012-10-01 2012-10-01 false Requirements for making requests. 612.3 Section... AVAILABILITY OF RECORDS AND INFORMATION § 612.3 Requirements for making requests. (a) Where to send a...

  2. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  3. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  4. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  5. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  6. 36 CFR 1008.13 - Requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Requests for access to records. 1008.13 Section 1008.13 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.13 Requests for access to records. The Privacy Act permits individuals, upon...

  7. 36 CFR 1008.13 - Requests for access to records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Requests for access to records. 1008.13 Section 1008.13 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.13 Requests for access to records. The Privacy Act permits individuals, upon...

  8. 36 CFR 1008.13 - Requests for access to records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Requests for access to records. 1008.13 Section 1008.13 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.13 Requests for access to records. The Privacy Act permits individuals, upon...

  9. 36 CFR 1008.13 - Requests for access to records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Requests for access to records. 1008.13 Section 1008.13 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.13 Requests for access to records. The Privacy Act permits individuals, upon...

  10. No Child Left Behind Act: Additional Assistance and Research on Effective Strategies Would Help Small Rural Districts. Report to Congressional Requesters. GAO-04-909

    ERIC Educational Resources Information Center

    Hickok, Eugene W.

    2004-01-01

    Congress has raised concerns about difficulties rural school districts face implementing the No Child Left Behind Act (NCLBA). This report describes: key challenges rural states and districts face; strategies rural districts have developed; expenditures and resources related to rural districts? compliance; and guidance and assistance from the…

  11. Workforce Investment Act: Improvements Needed in Performance Measures To Provide a More Accurate Picture of WIA's Effectiveness. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    Nilsen, Sigurd R.

    A study assessed effectiveness of Workforce Investment Act (WIA) of 1998 performance measures. Data were from a survey of WIA program administrators in 50 states; visits to 5 states; interviews with labor officials and national associations representing state and local workforce development officials; and a document review. Findings indicate…

  12. DNA Polymerase α (swi7) and the Flap Endonuclease Fen1 (rad2) Act Together in the S-Phase Alkylation Damage Response in S. pombe

    PubMed Central

    Koulintchenko, Milana; Vengrova, Sonya; Eydmann, Trevor; Arumugam, Prakash; Dalgaard, Jacob Z.

    2012-01-01

    Polymerase α is an essential enzyme mainly mediating Okazaki fragment synthesis during lagging strand replication. A specific point mutation in Schizosaccharomyces pombe polymerase α named swi7-1, abolishes imprinting required for mating-type switching. Here we investigate whether this mutation confers any genome-wide defects. We show that the swi7-1 mutation renders cells hypersensitive to the DNA damaging agents methyl methansulfonate (MMS), hydroxyurea (HU) and UV and incapacitates activation of the intra-S checkpoint in response to DNA damage. In addition we show that, in the swi7-1 background, cells are characterized by an elevated level of repair foci and recombination, indicative of increased genetic instability. Furthermore, we detect novel Swi1-, -Swi3- and Pol α- dependent alkylation damage repair intermediates with mobility on 2D-gel that suggests presence of single-stranded regions. Genetic interaction studies showed that the flap endonuclease Fen1 works in the same pathway as Pol α in terms of alkylation damage response. Fen1 was also required for formation of alkylation- damage specific repair intermediates. We propose a model to explain how Pol α, Swi1, Swi3 and Fen1 might act together to detect and repair alkylation damage during S-phase. PMID:23071723

  13. Prelinguistic Vocalizations Distinguish Pointing Acts

    ERIC Educational Resources Information Center

    Grunloh, Thomas; Liszkowski, Ulf

    2015-01-01

    The current study investigated whether point-accompanying characteristics, like vocalizations and hand shape, differentiate infants' underlying motives of prelinguistic pointing. We elicited imperative (requestive) and declarative (expressive and informative) pointing acts in experimentally controlled situations, and analyzed accompanying…

  14. Temporary alien workers seeking H-1B classification under the Immigration and Nationality Act--Immigration and Naturalization Service. Interim rule with requests for comments.

    PubMed

    1992-04-01

    This interim rule implements certain provisions of the Miscellaneous and Technical Immigration and Naturalization Amendments of 1991, Public Law 102-232, December 12, 1991, as it relates to aliens seeking nonimmigrant classification and admission to the United States under section 101(a)(15)(H) of the Immigration and Nationality Act (Act). Public Law 102-232 altered, among other things, the procedures for petitioning for H-1B nonimmigrants and established new eligibility criteria for foreign physicians seeking employment in the medical profession in the United States. This rule contains the new procedures required by the legislation and makes Service policy consistent with the intent of Congress. This rule sets forth the new filing procedures and eligibility standards and clarifies for businesses and the general public the requirements for classification and admission.

  15. Procedures for the handling of retaliation complaints under section 1558 of the Affordable Care Act. Interim final rule; request for comments.

    PubMed

    2013-02-27

    This document provides the interim final regulations governing the employee protection (whistleblower) provision of section 1558 of the Affordable Care Act, which added section 18C of the Fair Labor Standards Act, to provide protections to employees of health insurance issuers or other employers who may have been subject to retaliation for reporting potential violations of the law's consumer protections (e.g., the prohibition on denials of insurance due to pre-existing conditions) or affordability assistance provisions (e.g., access to health insurance premium tax credits). This interim rule establishes procedures and time frames for the handling of retaliation complaints under section 18C, including procedures and time frames for employee complaints to the Occupational Safety and Health Administration (OSHA), investigations by OSHA, appeals of OSHA determinations to an administrative law judge (ALJ) for a hearing de novo, hearings by ALJs, review of ALJ decisions by the Administrative Review Board (ARB) (acting on behalf of the Secretary of Labor), and judicial review of the Secretary's final decision.

  16. Procedures for the handling of retaliation complaints under section 1558 of the Affordable Care Act. Interim final rule; request for comments.

    PubMed

    2013-02-27

    This document provides the interim final regulations governing the employee protection (whistleblower) provision of section 1558 of the Affordable Care Act, which added section 18C of the Fair Labor Standards Act, to provide protections to employees of health insurance issuers or other employers who may have been subject to retaliation for reporting potential violations of the law's consumer protections (e.g., the prohibition on denials of insurance due to pre-existing conditions) or affordability assistance provisions (e.g., access to health insurance premium tax credits). This interim rule establishes procedures and time frames for the handling of retaliation complaints under section 18C, including procedures and time frames for employee complaints to the Occupational Safety and Health Administration (OSHA), investigations by OSHA, appeals of OSHA determinations to an administrative law judge (ALJ) for a hearing de novo, hearings by ALJs, review of ALJ decisions by the Administrative Review Board (ARB) (acting on behalf of the Secretary of Labor), and judicial review of the Secretary's final decision. PMID:23476992

  17. 77 FR 22765 - Proposed Information Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-17

    ... Reduction Act of 1995 (PRA95) (44 U.S.C. 3506(c)(2)(A)). This program helps to ensure that requested data... and structure in the future. Copies of the information collection request can be obtained...

  18. 21 CFR 20.23 - Request for existing records.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... request for records pursuant to the Freedom of Information Act, whether or not the Freedom of Information..., speeches, and educational materials, shall be furnished free of charge upon request as long as the...

  19. 75 FR 14582 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-26

    ... Notice of Proposed Information Collection Requests AGENCY: Department of Education. SUMMARY: The Acting Director, Information Collection Clearance Division, Regulatory Information Management Services, Office of Management, invites comments on the proposed information collection requests as required by the...

  20. 14 CFR 1212.201 - Requesting a record.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... request shall be processed as a Freedom of Information Act (FOIA) request under 14 CFR part 1206. If the... appropriate system manager, or, if unknown, to the NASA Headquarters or Field Installation Information...

  1. 32 CFR 1700.7 - Processing of requests for records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of Information Act Request.” (b) Electronic Reading Room. ODNI maintains an online FOIA Reading Room... on the amount of work and/or time needed to process the request, including through limits based...

  2. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the requester... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act,...

  3. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the requester... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act,...

  4. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the requester... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act,...

  5. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the requester... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act,...

  6. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the requester... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act,...

  7. 36 CFR 1008.14 - Requests for access to records: Submission.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the Privacy Act shall be in writing and addressed to Privacy Act Officer, The Presidio Trust, P.O. Box...: Submission. 1008.14 Section 1008.14 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.14 Requests for access to records: Submission. (a) Submission of requests. (1)...

  8. 77 FR 56861 - Agency Information Collection Activities: Proposed Collection, Comments Requested Extension of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... Requested Extension of a Currently Approved Collection Bioterrorism Preparedness Act: Entity/Individual.../collection: Federal Bureau of Investigation Bioterrorism Preparedness Act: Entity/Individual Information....

  9. 77 FR 56862 - Agency Information Collection Activities: Proposed Collection, Comments Requested; Extension of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... Requested; Extension of a Currently Approved Collection; Bioterrorism Preparedness Act: Entity/Individual.../collection: Federal Bureau of Investigation Bioterrorism Preparedness Act: Entity/Individual Information....

  10. 77 FR 56863 - Agency Information Collection Activities: Proposed Collection, Comments Requested Extension of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... Requested Extension of a Currently Approved Collection Bioterrorism Preparedness Act: Entity/Individual.../collection: Federal Bureau of Investigation Bioterrorism Preparedness Act: Entity/Individual Information....

  11. TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is a potential prognostic biomarker in lung cancer.

    PubMed

    Liu, Wen-bin; Han, Fei; Jiang, Xiao; Chen, Hong-qiang; Zhao, Huan; Liu, Yong; Li, Yong-hong; Huang, Chuanshu; Cao, Jia; Liu, Jin-yi

    2015-08-28

    Epigenetic silencing of tumour suppressors contributes to the development and progression of lung cancer. We recently found that TMEM196 was hypermethylated in lung cancer. This study aimed to clarify its epigenetic regulation, possible roles and clinical significance. TMEM196 methylation correlated with loss of protein expression in chemical-induced rat lung pathologic lesions and human lung cancer tissues and cell lines. 5-aza-2'-deoxycytidine restored TMEM196 expression. Moreover, TMEM196 hypermethylation was detected in 61.2% of primary lung tumours and found to be associated with poor differentiation and pathological stage of lung cancer. Functional studies showed that ectopic re-expression of TMEM196 in lung cancer cells inhibited cell proliferation, clonogenicity, cell motility and tumour formation. However, TMEM196 knockdown increased cell proliferation and inhibited apoptosis and cell-cycle arrest. These effects were associated with upregulation of p21 and Bax, and downregulation of cyclin D1, c-myc, CD44 and β-catenin. Kaplan-Meier survival curves showed that TMEM196 downregulation was significantly associated with shortened survival in lung cancer patients. Multivariate analysis showed that patients with TMEM196 expression had a better overall survival. Our results revealed for the first time that TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is an independent prognostic factor of lung cancer. PMID:26056045

  12. 78 FR 15377 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Requests To...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-11

    ... Benefit Plans Under the Davis-Bacon and Related Acts and Contract Work Hours and Safety Standards Act...; Requests To Approve Conformed Wage Classifications and Unconventional Fringe Benefit Plans Under the Davis-Bacon and Related Acts and Contract Work Hours and Safety Standards Act ACTION: Notice. SUMMARY:...

  13. 75 FR 81659 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Affordable...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ...; Affordable Care Act Enrollment Opportunity Notice--Prohibition on Lifetime Limits ACTION: Notice. SUMMARY... Administration (EBSA) sponsored information collection request (ICR) titled, ``Affordable Care Act Enrollment... INFORMATION: The Patient Protection and Affordable Care Act (the Affordable Care Act)......

  14. Rapid DNA Act of 2014

    THOMAS, 113th Congress

    Rep. Sensenbrenner, F. James, Jr. [R-WI-5

    2014-12-03

    12/18/2014 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, and Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  15. Analysis of S.1844, the Clear Skies Act of 2003; S. 843, the Clean Air Planning Act of 2003; and S. 366, the Clean Power Act of 2003

    EIA Publications

    2004-01-01

    Senator James M. Inhofe requested that the Energy Information Administration (EIA) undertake analysis of S.843, the Clean Air Planning Act of 2003, introduced by Senator Thomas Carper; S.366, the Clean Power Act of 2003, introduced by Senator James Jeffords; and S.1844, the Clear Skies Act of 2003, introduced by Senator James M. Inhofe. The EIA received this request on March 19, 2004. This Service Report responds to his request.

  16. 76 FR 30344 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... Federal Home Loan Bank Act (Bank Act) vests the management of each Federal Home Loan Bank (Bank) in its.... SUMMARY: In accordance with the Paperwork Reduction Act of 1995, Public Law 104-13, the Federal Housing...; Comment Request: ``Federal Home Loan Bank Directors, (No. 2011-N-05)'' in the subject line of the...

  17. Patient and family requests for hastened death.

    PubMed

    Abrahm, Janet L

    2008-01-01

    Patient and family requests for hastened death, upsetting as they are to the treating team, are usually a way for patients and their families to express their need for an increase in the intensity of communication, improved symptom control, or acknowledgment of an existential or spiritual crisis. Rarely do they represent the need for patients to control the time, place, and manner of their death. Using a hypothetical case study, this paper reviews the unspoken concerns underlying these requests; characteristics of patients who request a hastened death, and when and why they make the request; the Oregon Death with Dignity Act and its implementation since its passage in 1997; the effect these requests have on clinicians, their common reactions, and suggestions for self-care after such requests; techniques for responding to the requests and keeping the dialogue open with the patient and family; and the legal and ethical options available to clinicians outside of Oregon.

  18. 36 CFR 1192.37 - Stop request.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY GUIDELINES FOR TRANSPORTATION VEHICLES Buses, Vans and Systems § 1192.37 Stop request. (a) Where passengers may board or alight at multiple stops at their option... requesting stops and which alerts the driver that a mobility aid user wishes to disembark. Such a...

  19. 36 CFR 1192.37 - Stop request.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY GUIDELINES FOR TRANSPORTATION VEHICLES Buses, Vans and Systems § 1192.37 Stop request. (a) Where passengers may board or alight at multiple stops at their option... requesting stops and which alerts the driver that a mobility aid user wishes to disembark. Such a...

  20. 36 CFR 1192.37 - Stop request.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY GUIDELINES FOR TRANSPORTATION VEHICLES Buses, Vans and Systems § 1192.37 Stop request. (a) Where passengers may board or alight at multiple stops at their option... requesting stops and which alerts the driver that a mobility aid user wishes to disembark. Such a...

  1. 36 CFR 1192.37 - Stop request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY GUIDELINES FOR TRANSPORTATION VEHICLES Buses, Vans and Systems § 1192.37 Stop request. (a) Where passengers may board or alight at multiple stops at their option... requesting stops and which alerts the driver that a mobility aid user wishes to disembark. Such a...

  2. 76 FR 14106 - Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-15

    ... of Labor Statistics Proposed Collection, Comment Request ACTION: Notice. SUMMARY: The Department of... Reduction Act of 1995 (PRA95) . This program helps to ensure that requested data can be provided in the... Bureau of Labor Statistics (BLS) is soliciting comments concerning the proposed extension of...

  3. 49 CFR 38.37 - Stop request.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Stop request. 38.37 Section 38.37 Transportation Office of the Secretary of Transportation AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY SPECIFICATIONS FOR TRANSPORTATION VEHICLES Buses, Vans and Systems § 38.37 Stop request. (a) Where passengers...

  4. Detection of cis- and trans-acting Factors in DNA Structure-Induced Genetic Instability Using In silico and Cellular Approaches

    PubMed Central

    Wang, Guliang; Zhao, Junhua; Vasquez, Karen M.

    2016-01-01

    Sequences that can adopt alternative DNA structures (i.e., non-B DNA) are very abundant in mammalian genomes, and recent studies have revealed many important biological functions of non-B DNA structures in chromatin remodeling, DNA replication, transcription, and genetic instability. Here, we provide results from an in silico web-based search engine coupled with cell-based experiments to characterize the roles of non-B DNA conformations in genetic instability in eukaryotes. The purpose of this article is to illustrate strategies that can be used to identify and interrogate the biological roles of non-B DNA structures, particularly on genetic instability. We have included unpublished data using a short H-DNA-forming sequence from the human c-MYC promoter region as an example, and identified two different mechanisms of H-DNA-induced genetic instability in yeast and mammalian cells: a DNA replication-related model of mutagenesis; and a replication-independent cleavage model. Further, we identified candidate proteins involved in H-DNA-induced genetic instability by using a yeast genetic screen. A combination of in silico and cellular methods, as described here, should provide further insight into the contributions of non-B DNA structures in biological functions, genetic evolution, and disease development. PMID:27532010

  5. Detection of cis- and trans-acting Factors in DNA Structure-Induced Genetic Instability Using In silico and Cellular Approaches.

    PubMed

    Wang, Guliang; Zhao, Junhua; Vasquez, Karen M

    2016-01-01

    Sequences that can adopt alternative DNA structures (i.e., non-B DNA) are very abundant in mammalian genomes, and recent studies have revealed many important biological functions of non-B DNA structures in chromatin remodeling, DNA replication, transcription, and genetic instability. Here, we provide results from an in silico web-based search engine coupled with cell-based experiments to characterize the roles of non-B DNA conformations in genetic instability in eukaryotes. The purpose of this article is to illustrate strategies that can be used to identify and interrogate the biological roles of non-B DNA structures, particularly on genetic instability. We have included unpublished data using a short H-DNA-forming sequence from the human c-MYC promoter region as an example, and identified two different mechanisms of H-DNA-induced genetic instability in yeast and mammalian cells: a DNA replication-related model of mutagenesis; and a replication-independent cleavage model. Further, we identified candidate proteins involved in H-DNA-induced genetic instability by using a yeast genetic screen. A combination of in silico and cellular methods, as described here, should provide further insight into the contributions of non-B DNA structures in biological functions, genetic evolution, and disease development. PMID:27532010

  6. Detection of cis- and trans-acting Factors in DNA Structure-Induced Genetic Instability Using In silico and Cellular Approaches.

    PubMed

    Wang, Guliang; Zhao, Junhua; Vasquez, Karen M

    2016-01-01

    Sequences that can adopt alternative DNA structures (i.e., non-B DNA) are very abundant in mammalian genomes, and recent studies have revealed many important biological functions of non-B DNA structures in chromatin remodeling, DNA replication, transcription, and genetic instability. Here, we provide results from an in silico web-based search engine coupled with cell-based experiments to characterize the roles of non-B DNA conformations in genetic instability in eukaryotes. The purpose of this article is to illustrate strategies that can be used to identify and interrogate the biological roles of non-B DNA structures, particularly on genetic instability. We have included unpublished data using a short H-DNA-forming sequence from the human c-MYC promoter region as an example, and identified two different mechanisms of H-DNA-induced genetic instability in yeast and mammalian cells: a DNA replication-related model of mutagenesis; and a replication-independent cleavage model. Further, we identified candidate proteins involved in H-DNA-induced genetic instability by using a yeast genetic screen. A combination of in silico and cellular methods, as described here, should provide further insight into the contributions of non-B DNA structures in biological functions, genetic evolution, and disease development.

  7. 77 FR 35754 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... Reduction Act of 1995, Public Law 104-13 (44 U.S.C. 3506(c)(2)(A)). Currently, the Community Development....gov . Requests for additional information should be directed to Trefor Henry, Associate...

  8. 78 FR 72065 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... Act of 1995, the Department of Defense Education Activity announces a proposed public information... information technology. DATES: Consideration will be given to all comments received by January 31, 2014... of the Secretary Proposed Collection; Comment Request AGENCY: Department of Defense...

  9. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  10. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  11. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  12. 46 CFR 503.23 - Records available upon written request.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 9 2014-10-01 2014-10-01 false Records available upon written request. 503.23 Section... Information Act Procedures § 503.23 Records available upon written request. (a) The following Commission... procedures, upon request in writing addressed to the Office of the Secretary, Federal Maritime...

  13. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of identity,...

  14. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of identity,...

  15. 10 CFR 221.32 - Evaluation of DOD request.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 3 2014-01-01 2014-01-01 false Evaluation of DOD request. 221.32 Section 221.32 Energy... UNDER THE DEFENSE PRODUCTION ACT Administrative Procedures and Sanctions § 221.32 Evaluation of DOD request. (a) Upon receipt of a request from DOD for a priority rating as provided in § 221.31, it shall...

  16. 10 CFR 221.32 - Evaluation of DOD request.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 3 2012-01-01 2012-01-01 false Evaluation of DOD request. 221.32 Section 221.32 Energy... UNDER THE DEFENSE PRODUCTION ACT Administrative Procedures and Sanctions § 221.32 Evaluation of DOD request. (a) Upon receipt of a request from DOD for a priority rating as provided in § 221.31, it shall...

  17. 10 CFR 221.32 - Evaluation of DOD request.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 3 2013-01-01 2013-01-01 false Evaluation of DOD request. 221.32 Section 221.32 Energy... UNDER THE DEFENSE PRODUCTION ACT Administrative Procedures and Sanctions § 221.32 Evaluation of DOD request. (a) Upon receipt of a request from DOD for a priority rating as provided in § 221.31, it shall...

  18. 29 CFR 70.19 - Requests for records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of Records Under the Freedom of Information Act § 70.19 Requests for records. (a) How to make a... by mail, delivery service/courier, facsimile or e-mail. (b) To whom to direct requests. A request should be sent to the appropriate official/officer for the component that maintains the records at...

  19. 29 CFR 70.19 - Requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of Records Under the Freedom of Information Act § 70.19 Requests for records. (a) How to make a... by mail, delivery service/courier, facsimile or e-mail. (b) To whom to direct requests. A request should be sent to the appropriate official/officer for the component that maintains the records at...

  20. 29 CFR 70.19 - Requests for records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of Records Under the Freedom of Information Act § 70.19 Requests for records. (a) How to make a... by mail, delivery service/courier, facsimile or e-mail. (b) To whom to direct requests. A request should be sent to the appropriate official/officer for the component that maintains the records at...

  1. 39 CFR 3004.51 - Fees-request category.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 39 Postal Service 1 2012-07-01 2012-07-01 false Fees-request category. 3004.51 Section 3004.51 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT § 3004.51 Fees—request category. (a) The level of fee charged depends on the request category....

  2. 12 CFR 791.15 - Requests for open meeting.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Requests for open meeting. 791.15 Section 791... Sunshine Act § 791.15 Requests for open meeting. (a) Following any announcement that the Board intends to... the Board that the meeting or a portion of the meeting be open. The request shall be circulated to...

  3. 6 CFR 5.3 - Requirements for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Requirements for making requests. 5.3 Section 5... AND INFORMATION Freedom of Information Act § 5.3 Requirements for making requests. (a) How made and... the statute. If you are making a request for records about yourself, see § 5.21(d) for...

  4. 15 CFR 4.4 - Requirements for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Department of Commerce, operates under its own FOIA regulations at 37 CFR part 102, subpart A. Accordingly... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Requirements for making requests. 4.4... INFORMATION Freedom of Information Act § 4.4 Requirements for making requests. (a) A request for records...

  5. 5 CFR 9800.6 - Requirements for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Requirements for making requests. 9800.6 Section 9800.6 Administrative Personnel COUNCIL OF THE INSPECTORS GENERAL ON INTEGRITY AND EFFICIENCY FREEDOM OF INFORMATION ACT REGULATIONS § 9800.6 Requirements for making requests. (a) Requesters may...

  6. 45 CFR 612.3 - Requirements for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Privacy Act regulations at 45 CFR 613.2). (2) If you are making a request for personal information about... 45 Public Welfare 3 2014-10-01 2014-10-01 false Requirements for making requests. 612.3 Section... AVAILABILITY OF RECORDS AND INFORMATION § 612.3 Requirements for making requests. (a) Where to send a...

  7. 15 CFR 4.4 - Requirements for making requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Department of Commerce, operates under its own FOIA regulations at 37 CFR part 102, subpart A. Accordingly... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Requirements for making requests. 4.4... INFORMATION Freedom of Information Act § 4.4 Requirements for making requests. (a) A request for records...

  8. 45 CFR 612.3 - Requirements for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Privacy Act regulations at 45 CFR 613.2). (2) If you are making a request for personal information about... 45 Public Welfare 3 2013-10-01 2013-10-01 false Requirements for making requests. 612.3 Section... AVAILABILITY OF RECORDS AND INFORMATION § 612.3 Requirements for making requests. (a) Where to send a...

  9. 6 CFR 5.3 - Requirements for making requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Requirements for making requests. 5.3 Section 5... AND INFORMATION Freedom of Information Act § 5.3 Requirements for making requests. (a) How made and... the statute. If you are making a request for records about yourself, see § 5.21(d) for...

  10. 5 CFR 9800.6 - Requirements for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Requirements for making requests. 9800.6 Section 9800.6 Administrative Personnel COUNCIL OF THE INSPECTORS GENERAL ON INTEGRITY AND EFFICIENCY FREEDOM OF INFORMATION ACT REGULATIONS § 9800.6 Requirements for making requests. (a) Requesters may...

  11. 15 CFR 4.4 - Requirements for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Department of Commerce, operates under its own FOIA regulations at 37 CFR part 102, subpart A. Accordingly... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Requirements for making requests. 4.4... INFORMATION Freedom of Information Act § 4.4 Requirements for making requests. (a) A request for records...

  12. 6 CFR 5.3 - Requirements for making requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Requirements for making requests. 5.3 Section 5... AND INFORMATION Freedom of Information Act § 5.3 Requirements for making requests. (a) How made and... the statute. If you are making a request for records about yourself, see § 5.21(d) for...

  13. 15 CFR 4.4 - Requirements for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Department of Commerce, operates under its own FOIA regulations at 37 CFR part 102, subpart A. Accordingly... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Requirements for making requests. 4.4... INFORMATION Freedom of Information Act § 4.4 Requirements for making requests. (a) A request for records...

  14. 6 CFR 5.3 - Requirements for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Requirements for making requests. 5.3 Section 5... AND INFORMATION Freedom of Information Act § 5.3 Requirements for making requests. (a) How made and... the statute. If you are making a request for records about yourself, see § 5.21(d) for...

  15. 15 CFR 4.4 - Requirements for making requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Department of Commerce, operates under its own FOIA regulations at 37 CFR part 102, subpart A. Accordingly... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Requirements for making requests. 4.4... INFORMATION Freedom of Information Act § 4.4 Requirements for making requests. (a) A request for records...

  16. 6 CFR 5.3 - Requirements for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Requirements for making requests. 5.3 Section 5... AND INFORMATION Freedom of Information Act § 5.3 Requirements for making requests. (a) How made and... the statute. If you are making a request for records about yourself, see § 5.21(d) for...

  17. 14 CFR 1206.701 - Categories of requesters.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... are representatives of the news media. NASA shall provide documents to requesters in this category for... scientific institutions; representatives of the news media; and all other requesters. The Act prescribes specific levels of fees for each of these categories: (a) Commercial use requesters. When NASA receives...

  18. 14 CFR § 1206.701 - Categories of requesters.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... sought. (c) Requesters who are representatives of the news media. NASA shall provide documents to... noncommercial scientific institutions; representatives of the news media; and all other requesters. The Act prescribes specific levels of fees for each of these categories: (a) Commercial use requesters. When...

  19. 14 CFR 1206.701 - Categories of requesters.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... are representatives of the news media. NASA shall provide documents to requesters in this category for... scientific institutions; representatives of the news media; and all other requesters. The Act prescribes specific levels of fees for each of these categories: (a) Commercial use requesters. When NASA receives...

  20. 14 CFR 1206.701 - Categories of requesters.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... are representatives of the news media. NASA shall provide documents to requesters in this category for... scientific institutions; representatives of the news media; and all other requesters. The Act prescribes specific levels of fees for each of these categories: (a) Commercial use requesters. When NASA receives...

  1. 29 CFR 1921.5 - Motions and requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Motions and requests. 1921.5 Section 1921.5 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR... WORKERS' COMPENSATION ACT Prehearing Procedures § 1921.5 Motions and requests. Motions or requests...

  2. 4 CFR 201.5 - Requests for Board records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... RECOVERY ACCOUNTABILITY AND TRANSPARENCY BOARD PUBLIC INFORMATION AND REQUESTS § 201.5 Requests for Board records. (a) To request Board records, you may: (1) Write: FOIA Officer, Recovery Accountability and... of Information Act Request” on the letter and envelope or in the subject line of the e-mail...

  3. 45 CFR 2106.4 - Requests for copies of records.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Requests for copies of records. 2106.4 Section... FOR COMPLIANCE WITH 5 U.S.C. 552a, THE PRIVACY ACT OF 1974 § 2106.4 Requests for copies of records. Rules governing requests for copies of records are the same as those for the granting of access to...

  4. 6 CFR 5.6 - Responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Responses to requests. 5.6 Section 5.6 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act § 5.6 Responses to requests. (a) Acknowledgements of requests. On receipt of...

  5. 48 CFR 926.7003 - Review of the procurement request.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... potential for making Energy Policy Act awards. ... request. 926.7003 Section 926.7003 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SOCIOECONOMIC PROGRAMS OTHER SOCIOECONOMIC PROGRAMS Implementation of Section 3021 of the Energy Policy Act...

  6. 43 CFR 2.4 - Does where you send your request affect its processing?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... OF INFORMATION ACT; RECORDS AND TESTIMONY How To Make a Request § 2.4 Does where you send your request affect its processing? (a) A request to a particular bureau component (for example, a request addressed to a regional or field office) will be presumed to seek only records from that...

  7. 43 CFR 2.4 - Does where you send your request affect its processing?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... OF INFORMATION ACT; RECORDS AND TESTIMONY How To Make a Request § 2.4 Does where you send your request affect its processing? (a) A request to a particular bureau component (for example, a request addressed to a regional or field office) will be presumed to seek only records from that...

  8. Freedom of Information Act

    USGS Publications Warehouse

    Newman, D.J.

    2012-01-01

    The Freedom of Information Act( FOIA), 5 U.S.C.§ 552, as amended, generally provides that any person has a right to request access to Federal agency records. The USGS proactively promotes information disclosure as inherent to its mission of providing objective science to inform decisionmakers and the general public. USGS scientists disseminate up-to-date and historical scientific data that are critical to addressing national and global priorities.

  9. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  10. 32 CFR 806b.13 - Making a Request for Access.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Making a Request for Access. 806b.13 Section 806b.13 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION PRIVACY ACT PROGRAM Giving Access to Privacy Act Records § 806b.13 Making a Request for Access. Persons...

  11. 32 CFR 806b.13 - Making a Request for Access.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Making a Request for Access. 806b.13 Section 806b.13 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION PRIVACY ACT PROGRAM Giving Access to Privacy Act Records § 806b.13 Making a Request for Access. Persons...

  12. 32 CFR 806b.13 - Making a Request for Access.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Making a Request for Access. 806b.13 Section 806b.13 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION PRIVACY ACT PROGRAM Giving Access to Privacy Act Records § 806b.13 Making a Request for Access. Persons...

  13. 32 CFR 806b.13 - Making a Request for Access.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Making a Request for Access. 806b.13 Section 806b.13 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION PRIVACY ACT PROGRAM Giving Access to Privacy Act Records § 806b.13 Making a Request for Access. Persons...

  14. 32 CFR 806b.13 - Making a Request for Access.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Making a Request for Access. 806b.13 Section 806b.13 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION PRIVACY ACT PROGRAM Giving Access to Privacy Act Records § 806b.13 Making a Request for Access. Persons...

  15. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  16. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  17. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  18. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  19. 32 CFR 701.8 - Processing FOIA requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Additionally, written consent of the subject of the record is required for disclosure from a Privacy Act System... filing arrangements and existing retrieval systems, or unless the record contains sufficient Category II... in connection with a request for records concerning the requester, only records in Privacy Act...

  20. 36 CFR 1008.11 - Request for notification of existence of records: Submission.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... existence of records: Submission. 1008.11 Section 1008.11 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.11 Request for notification of existence of records: Submission. (a) Submission of requests. (1) Individuals desiring to determine under the Privacy Act whether a system...

  1. 36 CFR 1008.11 - Request for notification of existence of records: Submission.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... existence of records: Submission. 1008.11 Section 1008.11 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.11 Request for notification of existence of records: Submission. (a) Submission of requests. (1) Individuals desiring to determine under the Privacy Act whether a system...

  2. 36 CFR 1008.11 - Request for notification of existence of records: Submission.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... existence of records: Submission. 1008.11 Section 1008.11 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.11 Request for notification of existence of records: Submission. (a) Submission of requests. (1) Individuals desiring to determine under the Privacy Act whether a system...

  3. 36 CFR 1008.11 - Request for notification of existence of records: Submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... existence of records: Submission. 1008.11 Section 1008.11 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.11 Request for notification of existence of records: Submission. (a) Submission of requests. (1) Individuals desiring to determine under the Privacy Act whether a system...

  4. 36 CFR 1008.11 - Request for notification of existence of records: Submission.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... existence of records: Submission. 1008.11 Section 1008.11 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.11 Request for notification of existence of records: Submission. (a) Submission of requests. (1) Individuals desiring to determine under the Privacy Act whether a system...

  5. 78 FR 56225 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Soil...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-12

    ... activities as described in this ICR (77 FR 39699, July 5, 2012). EPA did not receive any public comments... AGENCY Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Soil...) for review and approval in accordance with the Paperwork Reduction Act: ``Soil Fumigant...

  6. 76 FR 28987 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  7. 75 FR 45121 - Agency Information Collection Request; 30-Day Public Comment Request; 30-Day Notice

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request; 30-Day Notice... Paperwork Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human...

  8. 75 FR 48969 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30 Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  9. 77 FR 29348 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  10. 76 FR 1616 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  11. 75 FR 32416 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ... Defense. WUSU 07 System name: USUHS Grievance Records (August 9, 1993; 58 FR 42304). Changes... Parkway, Aurora, CO 80011-9066. The request should contain the full name, address and the signature of the... Act Requester Service, 16401 Centretech Parkway, Aurora, CO 80011-9066. The request should contain...

  12. DNA nanomachines.

    PubMed

    Bath, Jonathan; Turberfield, Andrew J

    2007-05-01

    We are learning to build synthetic molecular machinery from DNA. This research is inspired by biological systems in which individual molecules act, singly and in concert, as specialized machines: our ambition is to create new technologies to perform tasks that are currently beyond our reach. DNA nanomachines are made by self-assembly, using techniques that rely on the sequence-specific interactions that bind complementary oligonucleotides together in a double helix. They can be activated by interactions with specific signalling molecules or by changes in their environment. Devices that change state in response to an external trigger might be used for molecular sensing, intelligent drug delivery or programmable chemical synthesis. Biological molecular motors that carry cargoes within cells have inspired the construction of rudimentary DNA walkers that run along self-assembled tracks. It has even proved possible to create DNA motors that move autonomously, obtaining energy by catalysing the reaction of DNA or RNA fuels.

  13. 76 FR 4377 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Affordable...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    ...; Affordable Care Act Enrollment Opportunity Notice Relating to Extension of Dependent Coverage ACTION: Notice... Administration (EBSA) sponsored information collection request (ICR) titled, ``Affordable Care Act Enrollment... Collection: Affordable Care Act Enrollment Opportunity Notice Relating to Extension of Dependent......

  14. 78 FR 13681 - Agency Information Collection Activities; Proposed Collection; Comment Request; Mammography...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... Collection; Comment Request; Mammography Quality Standards Act Requirements AGENCY: Food and Drug... associated with the Mammography Quality Standards Act requirements. DATES: Submit either electronic or... of information technology. Mammography Quality Standards Act Requirements--21 CFR Part 900...

  15. 77 FR 77112 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Workforce...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ...; Workforce Investment Act Management Information and Reporting System ACTION: Notice. SUMMARY: The Department... collection request (ICR) revision titled, ``Workforce Investment Act Management Information and Reporting...-year extension to the OMB PRA approval for the Workforce Investment Act Management Information...

  16. 78 FR 68096 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Quick...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... Workforce Investment Act Implementation AGENCY: Office of the Secretary, DOL. ACTION: Notice. SUMMARY: The... information collection request (ICR) titled, ``Quick Turnaround Surveys on Workforce Investment Act... quick turnaround surveys on Workforce Investment Act (WIA) related issues. Specifically, this ICR is...

  17. 77 FR 58398 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-20

    ...: Title IV-E Plan for Foster Care, Adoption Assistance, and, optional, Guardianship Assistance Programs... Social Security Act (the Act) for each public child welfare agency requesting Federal funding for foster care, adoption assistance and guardianship assistance under the Act. Section 479B of the Act...

  18. 76 FR 10006 - Proposed Information Collection; Comment Request; Economic Surveys of American Samoa, Guam, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-23

    ... National Oceanic and Atmospheric Administration Proposed Information Collection; Comment Request; Economic.../or continuing information collections, as required by the Paperwork Reduction Act of 1995. DATES... INFORMATION CONTACT: Requests for additional information or copies of the information collection...

  19. 76 FR 28951 - Proposed Information Collection; Comment Request; Data Collection for Compliance With Government...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... Economic Development Administration Proposed Information Collection; Comment Request; Data Collection for... on proposed and/or continuing information collections, as required by the Paperwork Reduction Act of... ). FOR FURTHER INFORMATION CONTACT: Requests for additional information or ] copies of the...

  20. 78 FR 19025 - Submission for Review; New Information Collection: Freedom of Information/Privacy Act Record...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... Request Form (INV 100) AGENCY: U.S. Office of Personnel Management. ACTION: 30-Day Notice and request for... the general public and other Federal agencies the opportunity to comment on a new information... Information/Privacy Act Record Request Form (INV 100). As required by the Paperwork Reduction Act of...

  1. [Mitochondrial DNA typing--a new level for solving identification problems in forensic medical expert identification of unidentified remains of victims of terrorist acts in Moscow and the armed conflict in the Chechen Republic].

    PubMed

    Ivanov, P L; Frolova, S A; Orekhov, V A; Iankovskiĭ, N K; Zemskova, E Iu

    2001-01-01

    Two large-scale episodes described in this paper reflect the first in Russia use of molecular genetic matrilinear markers (analysis of polymorphism of sequences of amplified fragments of mitochondrial DNA hypervariable locuses) in solution of a complex identification problem: forensic medical identification of unidentified fragments of victims of explosions of houses in Moscow in September, 1999, and of soldiers dead in the war conflict in the Chechen Republic in 1994-1996. The results of this work and methodological experience gained in it essentially extend the potentialities of expert studies as regards forensic medical identification of victims of large scale disasters, terroristic acts, and war conflicts.

  2. Budesonide epimer R or dexamethasone selectively inhibit platelet-activating factor-induced or interleukin 1β-induced DNA binding activity of cis-acting transcription factors and cyclooxygenase-2 gene expression in human epidermal keratinocytes

    PubMed Central

    Lukiw, Walter J.; Pelaez, Ricardo Palacios; Martinez, Jorge; Bazan, Nicolas G.

    1998-01-01

    To further understand the molecular mechanism of glucocorticoid action on gene expression, DNA-binding activities of the cis-acting transcription factors activator protein 1 (AP1), AP2, Egr1 (zif268), NF-κB, the signal transducers and activators of transcription proteins gamma interferon activation site (GAS), Sis-inducible element, and the TATA binding protein transcription factor II D (TFIID) were examined in human epidermal keratinocytes. The cytokine interleukin 1β (IL-1β) and platelet-activating factor (PAF), both potent mediators of inflammation, were used as triggers for gene expression. Budesonide epimer R (BUDeR) and dexamethasone (DEX) were studied as potential antagonists. BUDeR or DEX before IL-1β- or PAF-mediated gene induction elicited strong inhibition of AP1-, GAS-, and in particular NF-κB-DNA binding (P < 0.001, ANOVA). Only small effects were noted on AP2, Egr1 (zif268), and Sis-inducible element-DNA binding (P > 0.05). No significant effect was noted on the basal transcription factor TFIID recognition of TATA-containing core promoter sequences (P > 0.68). To test the hypothesis that changing cis-acting transcription factor binding activity may be involved in inflammatory-response related gene transcription, RNA message abundance for human cyclooxygenase (COX)-1 and -2 (E.C.1.14.99.1) was assessed in parallel by using reverse transcription–PCR. Although the COX-1 gene was found to be expressed at constitutively low levels, the TATA-containing COX-2 gene, which contains AP1-like, GAS, and NF-κB DNA-binding sites in its immediate promoter, was found to be strongly induced by IL-1β or PAF (P < 0.001). BUDeR and DEX both suppressed COX-2 RNA message generation; however, no correlation was associated with TFIID–DNA binding. These results suggest that on stimulation by mediators of inflammation, although the basal transcription machinery remains intact, modulation of cis-activating transcription factor AP1, GAS, and NF-κB-DNA binding by the

  3. 22 CFR 505.4 - Requirements and identification for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requests. 505.4 Section 505.4 Foreign Relations BROADCASTING BOARD OF GOVERNORS PRIVACY ACT REGULATION..., you may present your written request, fax it to (202) 260-4394 or mail it to the FOIA/Privacy Act... Avenue, SW, Washington, DC 20237. The FOIA/Privacy Act Office may be visited between the hours of 9...

  4. 36 CFR 1008.14 - Requests for access to records: Submission.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... subject to the Privacy Act shall be in writing and addressed to Privacy Act Officer, The Presidio Trust, P... records: Submission. 1008.14 Section 1008.14 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.14 Requests for access to records: Submission. (a) Submission of...

  5. 36 CFR 1008.14 - Requests for access to records: Submission.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... subject to the Privacy Act shall be in writing and addressed to Privacy Act Officer, The Presidio Trust, P... records: Submission. 1008.14 Section 1008.14 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.14 Requests for access to records: Submission. (a) Submission of...

  6. 36 CFR 1008.14 - Requests for access to records: Submission.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... subject to the Privacy Act shall be in writing and addressed to Privacy Act Officer, The Presidio Trust, P... records: Submission. 1008.14 Section 1008.14 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.14 Requests for access to records: Submission. (a) Submission of...

  7. 36 CFR 1008.14 - Requests for access to records: Submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... subject to the Privacy Act shall be in writing and addressed to Privacy Act Officer, The Presidio Trust, P... records: Submission. 1008.14 Section 1008.14 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.14 Requests for access to records: Submission. (a) Submission of...

  8. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  9. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  10. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  11. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  12. 43 CFR 2.10 - May you ask for the processing of your request to be expedited?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... request to be expedited? 2.10 Section 2.10 Public Lands: Interior Office of the Secretary of the Interior FREEDOM OF INFORMATION ACT; RECORDS AND TESTIMONY How To Make a Request § 2.10 May you ask for the processing of your request to be expedited? You may ask for the processing of your request to be...

  13. 43 CFR 2.10 - May you ask for the processing of your request to be expedited?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... request to be expedited? 2.10 Section 2.10 Public Lands: Interior Office of the Secretary of the Interior FREEDOM OF INFORMATION ACT; RECORDS AND TESTIMONY How To Make a Request § 2.10 May you ask for the processing of your request to be expedited? You may ask for the processing of your request to be...

  14. Managing DNA polymerases: coordinating DNA replication, DNA repair, and DNA recombination.

    PubMed

    Sutton, M D; Walker, G C

    2001-07-17

    Two important and timely questions with respect to DNA replication, DNA recombination, and DNA repair are: (i) what controls which DNA polymerase gains access to a particular primer-terminus, and (ii) what determines whether a DNA polymerase hands off its DNA substrate to either a different DNA polymerase or to a different protein(s) for the completion of the specific biological process? These questions have taken on added importance in light of the fact that the number of known template-dependent DNA polymerases in both eukaryotes and in prokaryotes has grown tremendously in the past two years. Most notably, the current list now includes a completely new family of enzymes that are capable of replicating imperfect DNA templates. This UmuC-DinB-Rad30-Rev1 superfamily of DNA polymerases has members in all three kingdoms of life. Members of this family have recently received a great deal of attention due to the roles they play in translesion DNA synthesis (TLS), the potentially mutagenic replication over DNA lesions that act as potent blocks to continued replication catalyzed by replicative DNA polymerases. Here, we have attempted to summarize our current understanding of the regulation of action of DNA polymerases with respect to their roles in DNA replication, TLS, DNA repair, DNA recombination, and cell cycle progression. In particular, we discuss these issues in the context of the Gram-negative bacterium, Escherichia coli, that contains a DNA polymerase (Pol V) known to participate in most, if not all, of these processes.

  15. Collaborative Dialogue in Learning Pragmatics: Pragmatic-Related Episodes as an Opportunity for Learning Request-Making

    ERIC Educational Resources Information Center

    Taguchi, Naoko; Kim, Youjin

    2016-01-01

    This study examined the effects of collaborative dialogue in learning the speech act of request. Seventy-four second-grade girls' junior high students were divided into three groups. The "collaborative group" (n = 25) received explicit metapragmatic information on request (request head act and modifications) followed by a dialogue…

  16. 19 CFR 201.29 - Commission disclosure of individual records, accounting of record disclosures, and requests for...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... disclosure required by 5 U.S.C. 552, the Privacy Act Officer shall keep an accurate accounting of: (1) The... individual requesting an accounting of disclosure of his or her records should make the request in writing to... and in the letter that it is a Privacy Act request for an accounting of disclosure of records....

  17. 75 FR 41887 - Request for Nominations of Members To Serve on the Bureau of Indian Education Advisory Board for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... Request for Nominations. SUMMARY: Pursuant to the Federal Advisory Committee Act, 5 U.S.C., Appendix 2... Advisory Committee Act regulations. Nomination Information (a) Nominations are requested from individuals... Bureau of Indian Affairs Request for Nominations of Members To Serve on the Bureau of Indian...

  18. 78 FR 48142 - Proposed Information Collection; Comment Request; Procedures for Considering Requests and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-07

    ... Act, and for providing relief under section 322(b) of the Act. In Proclamation No. 8341 (74 FR 4105... Requests and Comments From the Public Under the Textile Safeguard Provision of the United States-Peru Free... instructions should be directed to Laurie Mease, Office of Textiles and Apparel, U.S. Department of...

  19. 77 FR 31832 - Proposed Information Collection; Comment Request; Procedures for Considering Requests and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-30

    ... Act, and for providing relief under section 322(b) of the Act. In Proclamation No. 8818 (77 FR 29519... Requests and Comments From the Public for Textile and Apparel Safeguard Actions on Imports From Colombia... to Robert Carrigg, Office of Textiles and Apparel, U.S. Department of Commerce, Telephone:...

  20. 75 FR 30368 - Proposed Information Collection; Comment Request; Procedures for Considering Requests and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-01

    ... Act, and for providing relief under section 322(b) of the Act. In Proclamation No. 8341 (74 FR 4103... Requests and Comments From the Public for Textile and Apparel Safeguard Actions on Imports From Peru AGENCY... Carrigg, Office of Textiles and Apparel, U.S. Department of Commerce, Telephone: 202-482-2573, Fax:...

  1. 78 FR 20631 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Spill...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-05

    ... requested via the Federal Register (77 FR 74659) on December 17, 2012 during a 60-day comment period. This... derived from section 311(j)(1)(C) of the Clean Water Act, as amended by the Oil Pollution Act of 1990. EPA... attributed to adjustments for wage rates, unit costs, and the projected universe of facilities. John...

  2. 76 FR 77558 - Sunshine Act Meeting; Notice

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-13

    ... Counsel, at (202) 295-1500. Questions may be sent by electronic mail to FR_NOTICE_QUESTIONS@lsc.gov... or FR_NOTICE_QUESTIONS@lsc.gov , at least 2 business days in advance of the meeting. If a request is.... Consider and act on changes to LSC Bylaws necessitated by the DC Nonprofit Corporation Act of 2010....

  3. 77 FR 57013 - Privacy Act; Implementation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... of the Secretary 32 CFR Part 319 Privacy Act; Implementation AGENCY: Defense Intelligence Agency, DoD. ACTION: Direct final rule with request for comments. SUMMARY: The Defense Intelligence Agency is updating the Defense Intelligence Agency Privacy Act Program, by adding the (k)(2) exemption to...

  4. 77 FR 41801 - Agency Information Collection Activities: Proposed Collection, Comments Requested; Extension of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-16

    ... Requested; Extension of a Currently Approved Collection; Bioterrorism Preparedness Act: Entity/Individual.... (2) The title of the form/collection: Federal Bureau of Investigation Bioterrorism Preparedness...

  5. Genome-Wide Characterization of cis-Acting DNA Targets Reveals the Transcriptional Regulatory Framework of Opaque2 in Maize[OPEN

    PubMed Central

    Li, Chaobin; Qiao, Zhenyi; Qi, Weiwei; Wang, Qian; Yuan, Yue; Yang, Xi; Tang, Yuanping; Mei, Bing; Lv, Yuanda; Zhao, Han; Xiao, Han; Song, Rentao

    2015-01-01

    Opaque2 (O2) is a transcription factor that plays important roles during maize endosperm development. Mutation of the O2 gene improves the nutritional value of maize seeds but also confers pleiotropic effects that result in reduced agronomic quality. To reveal the transcriptional regulatory framework of O2, we studied the transcriptome of o2 mutants using RNA sequencing (RNA-Seq) and determined O2 DNA binding targets using chromatin immunoprecipitation coupled to high-throughput sequencing (ChIP-Seq). The RNA-Seq analysis revealed 1605 differentially expressed genes (DEGs) and 383 differentially expressed long, noncoding RNAs. The DEGs cover a wide range of functions related to nutrient reservoir activity, nitrogen metabolism, stress resistance, etc. ChIP-Seq analysis detected 1686 O2 DNA binding sites distributed over 1143 genes. Overlay of the RNA-Seq and ChIP-Seq results revealed 35 O2-modulated target genes. We identified four O2 binding motifs; among them, TGACGTGG appears to be the most conserved and strongest. We confirmed that, except for the 16- and 18-kD zeins, O2 directly regulates expression of all other zeins. O2 directly regulates two transcription factors, genes linked to carbon and amino acid metabolism and abiotic stress resistance. We built a hierarchical regulatory model for O2 that provides an understanding of its pleiotropic biological effects. PMID:25691733

  6. 20 CFR 402.15 - Relationship between the FOIA and the Privacy Act of 1974.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... “Individuals” and “system of records” are defined in the Privacy Act and in 20 CFR 401.25. (b) Requesting your... defined in the Privacy Act and in 20 CFR 401.25.) If we handle your request under the FOIA and the rules... Act of 1974. 402.15 Section 402.15 Employees' Benefits SOCIAL SECURITY ADMINISTRATION AVAILABILITY...

  7. 10 CFR 780.32 - Response and request for hearing.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Response and request for hearing. 780.32 Section 780.32 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.32 Response and request for hearing. Any...

  8. 10 CFR 780.32 - Response and request for hearing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Response and request for hearing. 780.32 Section 780.32 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.32 Response and request for hearing. Any...

  9. 10 CFR 780.32 - Response and request for hearing.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Response and request for hearing. 780.32 Section 780.32 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.32 Response and request for hearing. Any...

  10. 10 CFR 780.32 - Response and request for hearing.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Response and request for hearing. 780.32 Section 780.32 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.32 Response and request for hearing. Any...

  11. 10 CFR 780.32 - Response and request for hearing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Response and request for hearing. 780.32 Section 780.32 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.32 Response and request for hearing. Any...

  12. 7 CFR 1.172 - Motions and requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Motions and requests. 1.172 Section 1.172 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Rules of Practice Governing Cease and Desist Proceedings Under Section 2 of the Capper-Volstead Act § 1.172 Motions and requests. (a)...

  13. 76 FR 53906 - Proposed Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ...; Comment Request Title: ORR State Plan for Grants to States for Refugee Resettlement. OMB No. 0970-0351... IV, Sec. 412 of the Act] for each State agency requesting Federal funding for refugee resettlement under 8 U.S.C. 524 , including Refugee Cash and Medical Assistance, Refugee Social Services,...

  14. 42 CFR 405.1208 - Hospital requests expedited QIO review.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 2 2010-10-01 2010-10-01 false Hospital requests expedited QIO review. 405.1208... Reconsiderations of Provider Service Terminations, and Procedures for Inpatient Hospital Discharges § 405.1208 Hospital requests expedited QIO review. (a) General rule. (1) If the hospital (acting directly or...

  15. 32 CFR 1900.21 - Processing of requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 1900.21 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Cia Action on Foia... requester pursuant to 32 CFR 1900.33. In such event, the Agency will inform the requester in writing...

  16. 32 CFR 1900.21 - Processing of requests for records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 1900.21 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Cia Action on Foia... requester pursuant to 32 CFR 1900.33. In such event, the Agency will inform the requester in writing...

  17. 32 CFR 1900.21 - Processing of requests for records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 1900.21 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Cia Action on Foia... requester pursuant to 32 CFR 1900.33. In such event, the Agency will inform the requester in writing...

  18. 32 CFR 1900.21 - Processing of requests for records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 1900.21 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Cia Action on Foia... requester pursuant to 32 CFR 1900.33. In such event, the Agency will inform the requester in writing...

  19. 10 CFR 221.31 - Requests by DOD.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 3 2012-01-01 2012-01-01 false Requests by DOD. 221.31 Section 221.31 Energy DEPARTMENT... THE DEFENSE PRODUCTION ACT Administrative Procedures and Sanctions § 221.31 Requests by DOD. (a) When DOD finds that (1) a fuel supply shortage for DOD exists or is anticipated which would have...

  20. 10 CFR 221.31 - Requests by DOD.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 3 2014-01-01 2014-01-01 false Requests by DOD. 221.31 Section 221.31 Energy DEPARTMENT... THE DEFENSE PRODUCTION ACT Administrative Procedures and Sanctions § 221.31 Requests by DOD. (a) When DOD finds that (1) a fuel supply shortage for DOD exists or is anticipated which would have...

  1. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  2. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  3. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  4. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  5. 10 CFR 1304.105 - Requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Requests for access to records. 1304.105 Section 1304.105 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.105 Requests for access to records.... Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300; Arlington, VA 22201. (c)...

  6. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  7. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  8. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  9. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  10. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  11. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  12. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  13. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  14. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  15. 40 CFR 2.102 - Procedures for making requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Procedures for making requests. 2.102... Procedures for Disclosure of Records Under the Freedom of Information Act § 2.102 Procedures for making... writing. When making a request, you may specify a willingness to pay a greater or lesser amount. Should...

  16. 40 CFR 2.102 - Procedures for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Procedures for making requests. 2.102... Procedures for Disclosure of Records Under the Freedom of Information Act § 2.102 Procedures for making... writing. When making a request, you may specify a willingness to pay a greater or lesser amount. Should...

  17. 25 CFR 515.3 - Identification of individuals making requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Identification of individuals making requests. 515.3 Section 515.3 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR GENERAL PROVISIONS PRIVACY ACT PROCEDURES § 515.3 Identification of individuals making requests. (a) Any individual...

  18. 40 CFR 2.102 - Procedures for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Procedures for making requests. 2.102... Procedures for Disclosure of Records Under the Freedom of Information Act § 2.102 Procedures for making... writing. When making a request, you may specify a willingness to pay a greater or lesser amount. Should...

  19. 18 CFR 1301.3 - Requirements for making requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Requirements for making... PROCEDURES Freedom of Information Act § 1301.3 Requirements for making requests. (a) How made and addressed... as well—helpful in making your request. For additional information about the FOIA, you may...

  20. 18 CFR 1301.3 - Requirements for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Requirements for making... PROCEDURES Freedom of Information Act § 1301.3 Requirements for making requests. (a) How made and addressed... as well—helpful in making your request. For additional information about the FOIA, you may...

  1. 18 CFR 1301.3 - Requirements for making requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 2 2012-04-01 2012-04-01 false Requirements for making... PROCEDURES Freedom of Information Act § 1301.3 Requirements for making requests. (a) How made and addressed... as well—helpful in making your request. For additional information about the FOIA, you may...

  2. 40 CFR 2.102 - Procedures for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Procedures for making requests. 2.102... Procedures for Disclosure of Records Under the Freedom of Information Act § 2.102 Procedures for making... writing. When making a request, you may specify a willingness to pay a greater or lesser amount. Should...

  3. 25 CFR 515.3 - Identification of individuals making requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Identification of individuals making requests. 515.3 Section 515.3 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR GENERAL PROVISIONS PRIVACY ACT PROCEDURES § 515.3 Identification of individuals making requests. (a) Any individual...

  4. 25 CFR 515.3 - Identification of individuals making requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false Identification of individuals making requests. 515.3 Section 515.3 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR GENERAL PROVISIONS PRIVACY ACT PROCEDURES § 515.3 Identification of individuals making requests. (a) Any individual...

  5. 18 CFR 1301.3 - Requirements for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false Requirements for making... PROCEDURES Freedom of Information Act § 1301.3 Requirements for making requests. (a) How made and addressed... as well—helpful in making your request. For additional information about the FOIA, you may...

  6. 40 CFR 2.102 - Procedures for making requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Procedures for making requests. 2.102... Procedures for Disclosure of Records Under the Freedom of Information Act § 2.102 Procedures for making... writing. When making a request, you may specify a willingness to pay a greater or lesser amount. Should...

  7. 18 CFR 1301.3 - Requirements for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true Requirements for making... PROCEDURES Freedom of Information Act § 1301.3 Requirements for making requests. (a) How made and addressed... as well—helpful in making your request. For additional information about the FOIA, you may...

  8. 25 CFR 515.3 - Identification of individuals making requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Identification of individuals making requests. 515.3 Section 515.3 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR GENERAL PROVISIONS PRIVACY ACT PROCEDURES § 515.3 Identification of individuals making requests. (a) Any individual...

  9. 25 CFR 515.3 - Identification of individuals making requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Identification of individuals making requests. 515.3 Section 515.3 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR GENERAL PROVISIONS PRIVACY ACT PROCEDURES § 515.3 Identification of individuals making requests. (a) Any individual...

  10. 22 CFR 1101.6 - Requests for access to records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Requests for access to records. 1101.6 Section 1101.6 Foreign Relations INTERNATIONAL BOUNDARY AND WATER COMMISSION, UNITED STATES AND MEXICO, UNITED STATES SECTION PRIVACY ACT OF 1974 § 1101.6 Requests for access to records. (a) Any individual may...

  11. 10 CFR 1304.105 - Requests for access to records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Requests for access to records. 1304.105 Section 1304.105 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.105 Requests for access to records.... Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300; Arlington, VA 22201. (c)...

  12. 10 CFR 1304.105 - Requests for access to records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Requests for access to records. 1304.105 Section 1304.105 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.105 Requests for access to records.... Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300; Arlington, VA 22201. (c)...

  13. 40 CFR 2.104 - Responses to requests and appeals.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Responses to requests and appeals. 2.104 Section 2.104 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL PUBLIC INFORMATION Procedures for Disclosure of Records Under the Freedom of Information Act § 2.104 Responses to requests and appeals. (a) Unless the Agency and...

  14. 15 CFR 2016.0 - Requests for reviews

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Requests for reviews 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  15. 28 CFR 802.15 - Denial of request.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Denial of request. 802.15 Section 802.15 Judicial Administration COURT SERVICES AND OFFENDER SUPERVISION AGENCY FOR THE DISTRICT OF COLUMBIA DISCLOSURE OF RECORDS Privacy Act § 802.15 Denial of request. (a) Denial in whole or in part. If it...

  16. 28 CFR 802.15 - Denial of request.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Denial of request. 802.15 Section 802.15 Judicial Administration COURT SERVICES AND OFFENDER SUPERVISION AGENCY FOR THE DISTRICT OF COLUMBIA DISCLOSURE OF RECORDS Privacy Act § 802.15 Denial of request. (a) Denial in whole or in part. If it...

  17. 5 CFR 9301.17 - Requests to amend records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Requests to amend records. 9301.17 Section 9301.17 Administrative Personnel SPECIAL INSPECTOR GENERAL FOR AFGHANISTAN RECONSTRUCTION DISCLOSURE OF RECORDS AND INFORMATION Privacy Act § 9301.17 Requests to amend records. (a) Requirement...

  18. 5 CFR 9301.17 - Requests to amend records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Requests to amend records. 9301.17 Section 9301.17 Administrative Personnel SPECIAL INSPECTOR GENERAL FOR AFGHANISTAN RECONSTRUCTION DISCLOSURE OF RECORDS AND INFORMATION Privacy Act § 9301.17 Requests to amend records. (a) Requirement...

  19. 5 CFR 9301.16 - Requests for copies of records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Requests for copies of records. 9301.16 Section 9301.16 Administrative Personnel SPECIAL INSPECTOR GENERAL FOR AFGHANISTAN RECONSTRUCTION DISCLOSURE OF RECORDS AND INFORMATION Privacy Act § 9301.16 Requests for copies of records. Rules...

  20. 5 CFR 9301.16 - Requests for copies of records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Requests for copies of records. 9301.16 Section 9301.16 Administrative Personnel SPECIAL INSPECTOR GENERAL FOR AFGHANISTAN RECONSTRUCTION DISCLOSURE OF RECORDS AND INFORMATION Privacy Act § 9301.16 Requests for copies of records. Rules...

  1. 22 CFR 1101.6 - Requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Requests for access to records. 1101.6 Section 1101.6 Foreign Relations INTERNATIONAL BOUNDARY AND WATER COMMISSION, UNITED STATES AND MEXICO, UNITED STATES SECTION PRIVACY ACT OF 1974 § 1101.6 Requests for access to records. (a) Any individual may...

  2. 40 CFR 305.20 - Request for a hearing; contents.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., AND LIABILITY ACT (CERCLA) ADMINISTRATIVE HEARING PROCEDURES FOR CLAIMS AGAINST THE SUPERFUND Prehearing Procedures § 305.20 Request for a hearing; contents. (a) Within 30 days after receiving notice... for a Hearing with the Review Officer. The Request for a Hearing shall contain: (1) A statement of...

  3. 17 CFR 200.304 - Disclosure of requested records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ORGANIZATION; CONDUCT AND ETHICS; AND INFORMATION AND REQUESTS Regulations Pertaining to the Privacy of... Commission's Privacy Act Officer who initially will determine whether access will be granted, Provided... Counsel, whose zone of responsibility relates to the record requested (see 17 CFR 200.13 et seq.),...

  4. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  5. 5 CFR 1630.4 - Request for notification and access.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Request for notification and access. 1630.4 Section 1630.4 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD PRIVACY ACT REGULATIONS § 1630.4 Request for notification and access. (a) TSP records. (1) Records on TSP participants and the spouses, former spouses,...

  6. 15 CFR 2016.0 - Requests for reviews.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 3 2013-01-01 2013-01-01 false Requests for reviews. 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  7. 15 CFR 2016.0 - Requests for reviews

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 3 2011-01-01 2011-01-01 false Requests for reviews 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  8. 15 CFR 2016.0 - Requests for reviews.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 3 2012-01-01 2012-01-01 false Requests for reviews. 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  9. 15 CFR 2016.0 - Requests for reviews.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 3 2014-01-01 2014-01-01 false Requests for reviews. 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  10. 21 CFR 21.42 - Responses to requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 1 2013-04-01 2013-04-01 false Responses to requests. 21.42 Section 21.42 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.42... requester is not an “individual” under § 21.3(a), the letter shall so state. Where appropriate, the...

  11. 21 CFR 21.41 - Processing of requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 1 2012-04-01 2012-04-01 false Processing of requests. 21.41 Section 21.41 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.41 Processing of requests. (a) An individual or his guardian under § 21.75 shall not be required to show...

  12. 21 CFR 21.41 - Processing of requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 1 2013-04-01 2013-04-01 false Processing of requests. 21.41 Section 21.41 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.41 Processing of requests. (a) An individual or his guardian under § 21.75 shall not be required to show...

  13. 21 CFR 21.42 - Responses to requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 1 2011-04-01 2011-04-01 false Responses to requests. 21.42 Section 21.42 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.42... requester is not an “individual” under § 21.3(a), the letter shall so state. Where appropriate, the...

  14. 21 CFR 21.41 - Processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Processing of requests. 21.41 Section 21.41 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.41 Processing of requests. (a) An individual or his guardian under § 21.75 shall not be required to show...

  15. 21 CFR 21.42 - Responses to requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 1 2014-04-01 2014-04-01 false Responses to requests. 21.42 Section 21.42 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.42... requester is not an “individual” under § 21.3(a), the letter shall so state. Where appropriate, the...

  16. 21 CFR 21.42 - Responses to requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 1 2012-04-01 2012-04-01 false Responses to requests. 21.42 Section 21.42 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.42... requester is not an “individual” under § 21.3(a), the letter shall so state. Where appropriate, the...

  17. 21 CFR 21.41 - Processing of requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 1 2011-04-01 2011-04-01 false Processing of requests. 21.41 Section 21.41 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.41 Processing of requests. (a) An individual or his guardian under § 21.75 shall not be required to show...

  18. 21 CFR 21.41 - Processing of requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 1 2014-04-01 2014-04-01 false Processing of requests. 21.41 Section 21.41 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.41 Processing of requests. (a) An individual or his guardian under § 21.75 shall not be required to show...

  19. 21 CFR 21.42 - Responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Responses to requests. 21.42 Section 21.42 Food... PRIVACY Procedures for Notification of and Access to Records in Privacy Act Record Systems § 21.42... requester is not an “individual” under § 21.3(a), the letter shall so state. Where appropriate, the...

  20. 75 FR 15668 - Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-30

    ...; ] DEPARTMENT OF AGRICULTURE Rural Utilities Service Information Collection Activity; Comment Request AGENCY: Rural Utilities Service, USDA. ACTION: Notice and request for comments. SUMMARY: In accordance with the Paperwork Reduction Act of 1995 (44 U.S.C. Chapter 35, as amended), the Rural Utilities Service...

  1. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air Force elements must make reasonable efforts to find...

  2. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air Force elements must make reasonable efforts to find...

  3. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air Force elements must make reasonable efforts to find...

  4. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air Force elements must make reasonable efforts to find...

  5. 32 CFR 1800.21 - Processing of requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Processing of requests for records. 1800.21 Section 1800.21 National Defense Other Regulations Relating to National Defense NATIONAL COUNTERINTELLIGENCE CENTER PUBLIC ACCESS TO NACIC RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) NACIC Action On FOIA Requests § 1800.21 Processing of...

  6. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air Force elements must make reasonable efforts to find...

  7. 32 CFR 1900.21 - Processing of requests for records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requester pursuant to 32 CFR 1900.33. In such event, the Agency will inform the requester in writing and... AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Cia Action on Foia... taskings to the CIA components reasonably believed to hold responsive records. Effective October 2,...

  8. 39 CFR 3004.42 - Tracking of requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 39 Postal Service 1 2013-07-01 2013-07-01 false Tracking of requests. 3004.42 Section 3004.42 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT... that number to the person making the request. (b) Any person with a tracking number may call or...

  9. 39 CFR 3004.42 - Tracking of requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 39 Postal Service 1 2011-07-01 2011-07-01 false Tracking of requests. 3004.42 Section 3004.42 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT... that number to the person making the request. (b) Any person with a tracking number may call or...

  10. 39 CFR 3004.42 - Tracking of requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 39 Postal Service 1 2012-07-01 2012-07-01 false Tracking of requests. 3004.42 Section 3004.42 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT... that number to the person making the request. (b) Any person with a tracking number may call or...

  11. 6 CFR 5.4 - Responsibility for responding to requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Responsibility for responding to requests. 5.4 Section 5.4 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act § 5.4 Responsibility for responding to requests. (a)...

  12. 6 CFR 5.5 - Timing of responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Timing of responses to requests. 5.5 Section 5.5 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act § 5.5 Timing of responses to requests. (a) In general....

  13. 75 FR 12519 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION Notice of Proposed Information Collection Requests AGENCY: Department of Education. SUMMARY: The Acting... Department of Education is especially interested in public comment addressing the following issues: (1)...

  14. 49 CFR 599.601 - Procedures for requesting exception.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... CONSUMER ASSISTANCE TO RECYCLE AND SAVE ACT PROGRAM Requirements and Procedures for Exceptions § 599.601.... (b) Contents. The request must include paper copies of the following materials: (1) Explanation...

  15. 75 FR 31428 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION Notice of Proposed Information Collection Requests AGENCY: Department of Education. SUMMARY: The Acting... Department of Education is especially interested in public comment addressing the following issues: (1)...

  16. 32 CFR 320.4 - Procedures for requesting information.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... accordance with “DoD Freedom of Information Act Program Regulation” (32 CFR part 286). (g) Requests for... (CONTINUED) PRIVACY PROGRAM NATIONAL GEOSPATIAL-INTELLIGENCE AGENCY (NGA) PRIVACY § 320.4 Procedures...

  17. 32 CFR 320.4 - Procedures for requesting information.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... accordance with “DoD Freedom of Information Act Program Regulation” (32 CFR part 286). (g) Requests for... (CONTINUED) PRIVACY PROGRAM NATIONAL GEOSPATIAL-INTELLIGENCE AGENCY (NGA) PRIVACY § 320.4 Procedures...

  18. 77 FR 14770 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-13

    ... Notice of Proposed Information Collection Requests AGENCY: Department of Education. ACTION: Comment... information technology. Dated: March 7, 2012. Stephanie Valentine, Acting Director, Information Collection... for information should be addressed to U.S. Department of Education, 400 Maryland Avenue SW.,...

  19. 32 CFR 320.4 - Procedures for requesting information.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... accordance with “DoD Freedom of Information Act Program Regulation” (32 CFR part 286). (g) Requests for... Directive 7650.1 1 “GAO Access to Records”. 1 Copies may be obtained via Internet at...

  20. 32 CFR 320.4 - Procedures for requesting information.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... accordance with “DoD Freedom of Information Act Program Regulation” (32 CFR part 286). (g) Requests for... Directive 7650.1 1 “GAO Access to Records”. 1 Copies may be obtained via Internet at...

  1. 48 CFR 926.7003 - Review of the procurement request.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 1992 926.7003 Review of the procurement request. Any Energy Policy Act procurement, including basic research contracts with educational institutions, shall be reviewed in accordance with the Small...

  2. 48 CFR 926.7003 - Review of the procurement request.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 1992 926.7003 Review of the procurement request. Any Energy Policy Act procurement, including basic research contracts with educational institutions, shall be reviewed in accordance with the Small...

  3. 24 CFR 26.19 - Request for production of documents.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... to produce, and permit the party making the request, or someone acting on the requestor's behalf, to inspect, copy, test, or sample any designated documents—including writings, drawings, graphs,...

  4. 24 CFR 26.19 - Request for production of documents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... to produce, and permit the party making the request, or someone acting on the requestor's behalf, to inspect, copy, test, or sample any designated documents—including writings, drawings, graphs,...

  5. 24 CFR 26.19 - Request for production of documents.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... to produce, and permit the party making the request, or someone acting on the requestor's behalf, to inspect, copy, test, or sample any designated documents—including writings, drawings, graphs,...

  6. 24 CFR 26.19 - Request for production of documents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... to produce, and permit the party making the request, or someone acting on the requestor's behalf, to inspect, copy, test, or sample any designated documents—including writings, drawings, graphs,...

  7. 24 CFR 26.19 - Request for production of documents.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... to produce, and permit the party making the request, or someone acting on the requestor's behalf, to inspect, copy, test, or sample any designated documents—including writings, drawings, graphs,...

  8. 75 FR 43156 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ...The Director, Information Collection Clearance Division, Regulatory Information Management Services, Office of Management, invites comments on the proposed information collection requests as required by the Paperwork Reduction Act of...

  9. 32 CFR 320.4 - Procedures for requesting information.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... accordance with “DoD Freedom of Information Act Program Regulation” (32 CFR part 286). (g) Requests for... (CONTINUED) PRIVACY PROGRAM NATIONAL GEOSPATIAL-INTELLIGENCE AGENCY (NGA) PRIVACY § 320.4 Procedures...

  10. 16 CFR 1015.5 - Time limitation on responses to requests for records and requests for expedited processing.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... imminent threat to the life or physical safety of an individual; or (ii) With respect to a request made by... public concerning actual or alleged Federal Government activity. (2) Requesters for expedited processing...) of the Consumer Product Safety Act, 15 U.S.C. 2055(b), and the regulations implementing that...

  11. 7 CFR 28.120 - Expenses to be borne by party requesting classification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Expenses to be borne by party requesting... United States Cotton Standards Act Fees and Costs § 28.120 Expenses to be borne by party requesting... shall be borne by the party requesting classification....

  12. 7 CFR 28.120 - Expenses to be borne by party requesting classification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Expenses to be borne by party requesting... United States Cotton Standards Act Fees and Costs § 28.120 Expenses to be borne by party requesting... shall be borne by the party requesting classification....

  13. 7 CFR 28.120 - Expenses to be borne by party requesting classification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Expenses to be borne by party requesting... United States Cotton Standards Act Fees and Costs § 28.120 Expenses to be borne by party requesting... shall be borne by the party requesting classification....

  14. 7 CFR 28.120 - Expenses to be borne by party requesting classification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Expenses to be borne by party requesting... United States Cotton Standards Act Fees and Costs § 28.120 Expenses to be borne by party requesting... shall be borne by the party requesting classification....

  15. 7 CFR 28.120 - Expenses to be borne by party requesting classification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Expenses to be borne by party requesting... United States Cotton Standards Act Fees and Costs § 28.120 Expenses to be borne by party requesting... shall be borne by the party requesting classification....

  16. 32 CFR 1900.14 - Fee estimates (pre-request option).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 1900.14 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Filing of Foia Requests..., a requester may submit a request for a fee estimate. The Agency will endeavor within ten (10)...

  17. 32 CFR 1900.14 - Fee estimates (pre-request option).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 1900.14 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Filing of Foia Requests..., a requester may submit a request for a fee estimate. The Agency will endeavor within ten (10)...

  18. 32 CFR 1900.14 - Fee estimates (pre-request option).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 1900.14 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Filing of Foia Requests..., a requester may submit a request for a fee estimate. The Agency will endeavor within ten (10)...

  19. 32 CFR 1900.14 - Fee estimates (pre-request option).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 1900.14 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Filing of Foia Requests..., a requester may submit a request for a fee estimate. The Agency will endeavor within ten (10)...

  20. 32 CFR 1900.14 - Fee estimates (pre-request option).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 1900.14 National Defense Other Regulations Relating to National Defense CENTRAL INTELLIGENCE AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Filing of Foia Requests..., a requester may submit a request for a fee estimate. The Agency will endeavor within ten (10)...

  1. 15 CFR 4.24 - Procedures for making requests for records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Procedures for making requests for... GOVERNMENT INFORMATION Privacy Act § 4.24 Procedures for making requests for records. (a) Any individual... person. Each individual making a request in person shall be required to present satisfactory proof...

  2. 5 CFR 2606.202 - OGE or other agency action on requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... PROCEDURES PRIVACY ACT RULES Access to Records and Accounting of Disclosures § 2606.202 OGE or other agency action on requests. A response to a request for access should include the following: (a) A statement that there is a record or records as requested or a statement that there is not a record in the system...

  3. 5 CFR 2606.202 - OGE or other agency action on requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... PROCEDURES PRIVACY ACT RULES Access to Records and Accounting of Disclosures § 2606.202 OGE or other agency action on requests. A response to a request for access should include the following: (a) A statement that there is a record or records as requested or a statement that there is not a record in the system...

  4. 12 CFR 390.54 - Request for document discovery from parties.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 5 2012-01-01 2012-01-01 false Request for document discovery from parties... and Procedure in Adjudicatory Proceedings § 390.54 Request for document discovery from parties. (a... copying rate imposed under part 309 for requests under the Freedom of Information Act (5 U.S.C. 552)....

  5. 12 CFR 390.54 - Request for document discovery from parties.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 5 2014-01-01 2014-01-01 false Request for document discovery from parties... and Procedure in Adjudicatory Proceedings § 390.54 Request for document discovery from parties. (a... copying rate imposed under part 309 for requests under the Freedom of Information Act (5 U.S.C. 552)....

  6. 12 CFR 390.54 - Request for document discovery from parties.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 5 2013-01-01 2013-01-01 false Request for document discovery from parties... and Procedure in Adjudicatory Proceedings § 390.54 Request for document discovery from parties. (a... copying rate imposed under part 309 for requests under the Freedom of Information Act (5 U.S.C. 552)....

  7. 22 CFR 505.8 - Agency review of requests for changes.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Agency review of requests for changes. 505.8 Section 505.8 Foreign Relations BROADCASTING BOARD OF GOVERNORS PRIVACY ACT REGULATION § 505.8 Agency review of requests for changes. (a) In reviewing a record in response to a request to amend or correct...

  8. 22 CFR 505.8 - Agency review of requests for changes.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Agency review of requests for changes. 505.8 Section 505.8 Foreign Relations BROADCASTING BOARD OF GOVERNORS PRIVACY ACT REGULATION § 505.8 Agency review of requests for changes. (a) In reviewing a record in response to a request to amend or correct...

  9. 22 CFR 505.8 - Agency review of requests for changes.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 2 2013-04-01 2009-04-01 true Agency review of requests for changes. 505.8 Section 505.8 Foreign Relations BROADCASTING BOARD OF GOVERNORS PRIVACY ACT REGULATION § 505.8 Agency review of requests for changes. (a) In reviewing a record in response to a request to amend or correct...

  10. 22 CFR 505.8 - Agency review of requests for changes.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 2 2012-04-01 2009-04-01 true Agency review of requests for changes. 505.8 Section 505.8 Foreign Relations BROADCASTING BOARD OF GOVERNORS PRIVACY ACT REGULATION § 505.8 Agency review of requests for changes. (a) In reviewing a record in response to a request to amend or correct...

  11. 21 CFR 516.52 - Availability for public disclosure of data and information in requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Availability for public disclosure of data and information in requests. (a) FDA will not publicly disclose the existence of a request for MUMS-drug designation under section 573 of the act prior to final FDA action on... acknowledged, no data or information in the request are available for public disclosure prior to final...

  12. 21 CFR 516.52 - Availability for public disclosure of data and information in requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Availability for public disclosure of data and information in requests. (a) FDA will not publicly disclose the existence of a request for MUMS-drug designation under section 573 of the act prior to final FDA action on... acknowledged, no data or information in the request are available for public disclosure prior to final...

  13. 17 CFR 200.307 - Review of requests for amendment or correction.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... COMMISSION ORGANIZATION; CONDUCT AND ETHICS; AND INFORMATION AND REQUESTS Regulations Pertaining to the... making the request in writing of the extent to which it has been granted; (2) amend or correct the record... in part, the Privacy Act Officer will: (1) Promptly advise the individual making the request...

  14. 36 CFR 1008.17 - Requests for access to records: Special situations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Requests for access to records: Special situations. 1008.17 Section 1008.17 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.17 Requests for access to records: Special situations. (a)...

  15. 36 CFR 1008.17 - Requests for access to records: Special situations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Requests for access to records: Special situations. 1008.17 Section 1008.17 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.17 Requests for access to records: Special situations. (a)...

  16. 36 CFR 1008.17 - Requests for access to records: Special situations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Requests for access to records: Special situations. 1008.17 Section 1008.17 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.17 Requests for access to records: Special situations. (a) Medical...

  17. 36 CFR 1008.17 - Requests for access to records: Special situations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Requests for access to records: Special situations. 1008.17 Section 1008.17 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.17 Requests for access to records: Special situations. (a)...

  18. 36 CFR 1008.17 - Requests for access to records: Special situations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Requests for access to records: Special situations. 1008.17 Section 1008.17 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.17 Requests for access to records: Special situations. (a)...

  19. 39 CFR 3004.40 - Hard copy requests for records and for expedited processing.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Hard copy requests for records and for expedited... FREEDOM OF INFORMATION ACT § 3004.40 Hard copy requests for records and for expedited processing. (a) A hard copy request for records must: (1) Be in writing; (2) Include the name and address of...

  20. 5 CFR 2606.202 - OGE or other agency action on requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... records; (b) The method of access (if a copy of all the records requested is not provided with the... PROCEDURES PRIVACY ACT RULES Access to Records and Accounting of Disclosures § 2606.202 OGE or other agency action on requests. A response to a request for access should include the following: (a) A statement...

  1. 29 CFR 2701.4 - Request to open or close meeting.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... GOVERNMENT IN THE SUNSHINE ACT REGULATIONS § 2701.4 Request to open or close meeting. Any person may request that the Commission open a meeting that it has earlier decided to close. Any person whose interest may... 29 Labor 9 2010-07-01 2010-07-01 false Request to open or close meeting. 2701.4 Section...

  2. Inhibitor of differentiation 4 (ID4) acts as an inhibitor of ID-1, -2 and -3 and promotes basic helix loop helix (bHLH) E47 DNA binding and transcriptional activity.

    PubMed

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-05-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID-interactions.

  3. Inhibitor of Differentiation 4 (ID4) Acts as an Inhibitor of ID-1, -2 and -3 and Promotes basic Helix Loop Helix (bHLH) E47 DNA Binding and Transcriptional Activity

    PubMed Central

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-01-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID- interactions. PMID:25778840

  4. ACT: Acting Out Central Theme.

    ERIC Educational Resources Information Center

    Kise, Joan Duff

    1982-01-01

    The author describes ACT (Acting Out Central Theme), a method for dealing with psychomotor, cognitive, and affective domains in slow readers. The ACT approach involves three sessions which focus on discussion of a theme such as friendship, presentaton of the theme as a skit, and assignment of topics to individual students. (SW)

  5. Identification of a major cis-acting DNA element controlling the bidirectionally transcribed penicillin biosynthesis genes acvA (pcbAB) and ipnA (pcbC) of Aspergillus nidulans.

    PubMed Central

    Bergh, K T; Litzka, O; Brakhage, A A

    1996-01-01

    The beta-lactam antibiotic penicillin is produced as a secondary metabolite by some filamentous fungi. In this study, the molecular regulation of the Aspergillus (Emericella) nidulans penicillin biosynthesis genes acvA (pcbAB) and ipnA (pcbC) was analyzed. acvA and ipnA are divergently oriented and separated by an intergenic region of 872 bp. Translational fusions of acvA and ipnA with the two Escherichia coli reporter genes lacZ and uidA enabled us to measure the regulation of both genes simultaneously. A moving-window analysis of the 872-bp intergenic region indicated that the divergently oriented promoters are, at least in part, overlapping and share common regulatory elements. Removal of nucleotides -353 to -432 upstream of the acvA gene led to a 10-fold increase of acvA-uidA expression and simultaneously to a reduction of ipnA-lacZ expression to about 30%. Band shift assays and methyl interference analysis using partially purified protein extracts revealed that a CCAAT-containing DNA element within this region was specifically bound by a protein (complex), which we designated PENR1, for penicillin regulator. Deletion of 4 bp within the identified protein binding site caused the same contrary effects on acvA and ipnA expression as observed for all of the deletion clones which lacked nucleotides -353 to -432. The PENR1 binding site thus represents a major cis-acting DNA element. The intergenic regions of the corresponding genes of the beta-lactam-producing fungi Penicillium chrysogenum and Acremonium chrysogenum also diluted the complex formed between the A. nidulans probe and PENR1 in vitro, suggesting that these beta-lactam biosynthesis genes are regulated by analogous DNA elements and proteins. PMID:8682797

  6. Ancient DNA reveals selection acting on genes associated with hypoxia response in pre-Columbian Peruvian Highlanders in the last 8500 years

    PubMed Central

    Fehren-Schmitz, Lars; Georges, Lea

    2016-01-01

    Archaeological evidence shows that humans began living in the high altitude Andes approximately 12,000 years ago. Andean highlanders are known to have developed the most complex societies of pre-Columbian South America despite challenges to their health and reproductive success resulting from chronic exposure to hypoxia. While the physiological adaptations to this environmental stressor are well studied in contemporary Andean highlanders, the molecular evolutionary processes associated with such adaptations remain unclear. We aim to better understand how humans managed to demographically establish in this harsh environment by addressing a central question: did exposure to hypoxia drive adaptation via natural selection within Andean populations or did an existing phenotype –characterized by reduced susceptibility to hypoxic stress–enable human settlement of the Andes? We genotyped three variable loci within the NOS3 and EGLN1 genes previously associated with adaptation to high altitude in 150 ancient human DNA samples from Peruvian high altitude and coastal low altitude sites in a time frame between ~8500–560 BP. We compare the data of 109 successful samples to forward simulations of genetic drift with natural selection and find that selection, rather than drift, explains the gradual frequency changes observed in the highland populations for two of the three SNPs. PMID:26996763

  7. 76 FR 10624 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Employment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-25

    ... additional information, see the related notice published in the Federal Register on September 27, 2010 (75 FR... Requirement in this request: Workforce Investment Act Title IB, Wagner-Peysner Act, Trade...

  8. 75 FR 48973 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; National...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ... Review; Comment Request; National Survey of Older Americans Act Title III Service Recipients AGENCY... information to OMB for review and clearance. The National Survey of Older Americans Act Title III...

  9. 75 FR 11542 - Agency Information Collection Activities; Proposed Collection; Comment Request; Mammography...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-11

    ... Collection; Comment Request; Mammography Quality Standards Act Requirements AGENCY: Food and Drug... Mammography Quality Standards Act requirements. DATES: Submit written or electronic comments on the collection... techniques, when appropriate, and other forms of information technology. The Mammography Quality...

  10. NADH:Cytochrome b5 Reductase and Cytochrome b5 Can Act as Sole Electron Donors to Human Cytochrome P450 1A1-Mediated Oxidation and DNA Adduct Formation by Benzo[a]pyrene

    PubMed Central

    2016-01-01

    Benzo[a]pyrene (BaP) is a human carcinogen that covalently binds to DNA after activation by cytochrome P450 (P450). Here, we investigated whether NADH:cytochrome b5 reductase (CBR) in the presence of cytochrome b5 can act as sole electron donor to human P450 1A1 during BaP oxidation and replace the canonical NADPH:cytochrome P450 reductase (POR) system. We also studied the efficiencies of the coenzymes of these reductases, NADPH as a coenzyme of POR, and NADH as a coenzyme of CBR, to mediate BaP oxidation. Two systems containing human P450 1A1 were utilized: human recombinant P450 1A1 expressed with POR, CBR, epoxide hydrolase, and cytochrome b5 in Supersomes and human recombinant P450 1A1 reconstituted with POR and/or with CBR and cytochrome b5 in liposomes. BaP-9,10-dihydrodiol, BaP-7,8-dihydrodiol, BaP-1,6-dione, BaP-3,6-dione, BaP-9-ol, BaP-3-ol, a metabolite of unknown structure, and two BaP-DNA adducts were generated by the P450 1A1-Supersomes system, both in the presence of NADPH and in the presence of NADH. The major BaP-DNA adduct detected by 32P-postlabeling was characterized as 10-(deoxyguanosin-N2-yl)-7,8,9-trihydroxy-7,8,9,10-tetrahydro-BaP (assigned adduct 1), while the minor adduct is probably a guanine adduct derived from 9-hydroxy-BaP-4,5-epoxide (assigned adduct 2). BaP-3-ol as the major metabolite, BaP-9-ol, BaP-1,6-dione, BaP-3,6-dione, an unknown metabolite, and adduct 2 were observed in the system using P450 1A1 reconstituted with POR plus NADPH. When P450 1A1 was reconstituted with CBR and cytochrome b5 plus NADH, BaP-3-ol was the predominant metabolite too, and an adduct 2 was also generated. Our results demonstrate that the NADH/cytochrome b5/CBR system can act as the sole electron donor both for the first and second reduction of P450 1A1 during the oxidation of BaP in vitro. They suggest that NADH-dependent CBR can replace NADPH-dependent POR in the P450 1A1-catalyzed metabolism of BaP. PMID:27404282

  11. NADH:Cytochrome b5 Reductase and Cytochrome b5 Can Act as Sole Electron Donors to Human Cytochrome P450 1A1-Mediated Oxidation and DNA Adduct Formation by Benzo[a]pyrene.

    PubMed

    Stiborová, Marie; Indra, Radek; Moserová, Michaela; Frei, Eva; Schmeiser, Heinz H; Kopka, Klaus; Philips, David H; Arlt, Volker M

    2016-08-15

    Benzo[a]pyrene (BaP) is a human carcinogen that covalently binds to DNA after activation by cytochrome P450 (P450). Here, we investigated whether NADH:cytochrome b5 reductase (CBR) in the presence of cytochrome b5 can act as sole electron donor to human P450 1A1 during BaP oxidation and replace the canonical NADPH:cytochrome P450 reductase (POR) system. We also studied the efficiencies of the coenzymes of these reductases, NADPH as a coenzyme of POR, and NADH as a coenzyme of CBR, to mediate BaP oxidation. Two systems containing human P450 1A1 were utilized: human recombinant P450 1A1 expressed with POR, CBR, epoxide hydrolase, and cytochrome b5 in Supersomes and human recombinant P450 1A1 reconstituted with POR and/or with CBR and cytochrome b5 in liposomes. BaP-9,10-dihydrodiol, BaP-7,8-dihydrodiol, BaP-1,6-dione, BaP-3,6-dione, BaP-9-ol, BaP-3-ol, a metabolite of unknown structure, and two BaP-DNA adducts were generated by the P450 1A1-Supersomes system, both in the presence of NADPH and in the presence of NADH. The major BaP-DNA adduct detected by (32)P-postlabeling was characterized as 10-(deoxyguanosin-N(2)-yl)-7,8,9-trihydroxy-7,8,9,10-tetrahydro-BaP (assigned adduct 1), while the minor adduct is probably a guanine adduct derived from 9-hydroxy-BaP-4,5-epoxide (assigned adduct 2). BaP-3-ol as the major metabolite, BaP-9-ol, BaP-1,6-dione, BaP-3,6-dione, an unknown metabolite, and adduct 2 were observed in the system using P450 1A1 reconstituted with POR plus NADPH. When P450 1A1 was reconstituted with CBR and cytochrome b5 plus NADH, BaP-3-ol was the predominant metabolite too, and an adduct 2 was also generated. Our results demonstrate that the NADH/cytochrome b5/CBR system can act as the sole electron donor both for the first and second reduction of P450 1A1 during the oxidation of BaP in vitro. They suggest that NADH-dependent CBR can replace NADPH-dependent POR in the P450 1A1-catalyzed metabolism of BaP. PMID:27404282

  12. 77 FR 6090 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Office of the Secretary Proposed Collection; Comment Request AGENCY: Office of the Under Secretary of Defense for Personnel and Readiness, DoD. ACTION: Notice. In compliance with Section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office...

  13. Requests Win More Leeway under NCLB

    ERIC Educational Resources Information Center

    Olson, Lynn

    2005-01-01

    This article talks about the approval given by the Education Department to 16 states that requested changes to accountability plans under the No Child Left Behind Act. Federal officials had sent decision letters to these states. Another 31 states are awaiting such letters, although many have received oral approvals or denials. This article…

  14. 78 FR 51170 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Office of the Secretary Proposed Collection; Comment Request AGENCY: Defense Finance and Accounting Service (DFAS), DoD. ACTION: Notice. In compliance with Section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the DFAS announces a proposed...

  15. 77 FR 71176 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-29

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF DEFENSE Office of the Secretary Proposed Collection; Comment Request AGENCY: Office of the Assistant Secretary of Defense for Health Affairs, DoD. ACTION: Notice. In compliance with Section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of...

  16. Supportive Discourse Moves in Persian Requests

    ERIC Educational Resources Information Center

    Nodoushan, Mohammad Ali Salmani; Allami, Hamid

    2011-01-01

    This paper reports the findings of a study designed to investigate the types of supportive discourse moves employed by Persian speakers in their Requestive Speech Acts. 372 respondents took a Discourse Completion Test (DCT) with six scenarios ranging from formal to informal degrees of Perceived Situational Seriousness, and returned 2232 Requestive…

  17. 75 FR 22569 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-29

    ... Department of the Navy Proposed Collection; Comment Request AGENCY: Marine Corps Marathon, Marine Corps Base... the Paperwork Reduction Act of 1995, the Marine Corps Marathon, Marine Corps Base Quantico announces..., please write to the Marine Corps Marathon Office, Attn: Angela Huff, P.O. Box 188, Quantico, VA 22134,...

  18. 78 FR 70929 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-27

    ... Department of the Army Proposed Collection; Comment Request AGENCY: Office of the Administrative Assistant to the Secretary of the Army, DoD. ACTION: Notice. SUMMARY: In compliance with Section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Department of the Army announces a proposed...

  19. 76 FR 15351 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-21

    ...) of the Paperwork Reduction Act of 1995 which provides opportunity for public comment on new or... information collection: Request for Internet Services, OMB 3220-0198. The RRB uses a Personal Identification Number (PIN)/Password system that allows RRB customers to conduct business with the agency...

  20. 78 FR 63970 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-25

    ... Department of the Army Proposed Collection; Comment Request AGENCY: Office of the Administrative Assistant to the Secretary of the Army (OAA-RPA), DoD. ACTION: Notice. SUMMARY: In compliance with Section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Department of the Army announces a proposed...