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Sample records for act request dna

  1. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  2. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  3. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  4. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  5. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  6. 28 CFR 513.60 - Freedom of Information Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Freedom of Information Act requests. 513... AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.60 Freedom of Information Act requests. Requests for any Bureau record (including...

  7. 28 CFR 513.60 - Freedom of Information Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Freedom of Information Act requests. 513... AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.60 Freedom of Information Act requests. Requests for any Bureau record (including...

  8. 28 CFR 513.60 - Freedom of Information Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Freedom of Information Act requests. 513... AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.60 Freedom of Information Act requests. Requests for any Bureau record (including...

  9. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR 16... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Privacy Act requests by inmates....

  10. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR 16... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Privacy Act requests by inmates....

  11. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR 16... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Privacy Act requests by inmates....

  12. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR 16... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Privacy Act requests by inmates....

  13. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR 16.... See §§ 513.61 through 513.68. Freedom of Information Act Requests for Information...

  14. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for declassification that are submitted under the provisions of the Freedom of Information Act, as amended, (See §...

  15. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for declassification that are submitted under the provisions of the Freedom of Information Act, as amended, (See §...

  16. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for declassification that are submitted under the provisions of the Freedom of Information Act, as amended, (See §...

  17. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for declassification that are submitted under the provisions of the Freedom of Information Act, as amended, (See §...

  18. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.6 Procedures for Privacy Act requests. (a) Any individual may— (1)...

  19. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.6 Procedures for Privacy Act requests. (a) Any individual may— (1)...

  20. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.6 Procedures for Privacy Act requests. (a) Any individual may— (1)...

  1. 6 CFR 1002.3 - Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Privacy Act requests. 1002.3 Section 1002.3 Domestic Security PRIVACY AND CIVIL LIBERTIES OVERSIGHT BOARD IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 1002.3 Privacy Act requests. (a) Requests to determine if you are the subject of a record. You...

  2. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.6 Procedures for Privacy Act requests. (a) Any individual may— (1)...

  3. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.6 Procedures for Privacy Act requests. (a) Any individual may— (1)...

  4. 28 CFR 802.5 - Freedom of Information Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Freedom of Information Act requests. 802... DISTRICT OF COLUMBIA DISCLOSURE OF RECORDS Freedom of Information Act § 802.5 Freedom of Information Act...) ordinarily will be processed pursuant to the Freedom of Information Act, 5 U.S.C. 552. Your request must...

  5. 28 CFR 802.5 - Freedom of Information Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Freedom of Information Act requests. 802... DISTRICT OF COLUMBIA DISCLOSURE OF RECORDS Freedom of Information Act § 802.5 Freedom of Information Act...) ordinarily will be processed pursuant to the Freedom of Information Act, 5 U.S.C. 552. Your request must...

  6. 28 CFR 802.5 - Freedom of Information Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Freedom of Information Act requests. 802... DISTRICT OF COLUMBIA DISCLOSURE OF RECORDS Freedom of Information Act § 802.5 Freedom of Information Act...) ordinarily will be processed pursuant to the Freedom of Information Act, 5 U.S.C. 552. Your request must...

  7. 42 CFR 85.4 - Acting on requests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Acting on requests. 85.4 Section 85.4 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OCCUPATIONAL SAFETY AND HEALTH RESEARCH AND RELATED ACTIVITIES REQUESTS FOR HEALTH HAZARD EVALUATIONS § 85.4 Acting on requests. (a)...

  8. 42 CFR 90.5 - Acting on requests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Acting on requests. 90.5 Section 90.5 Public Health... PROCEDURES § 90.5 Acting on requests. (a) Upon receipt of a request for a health assessment submitted under... justify conducting a health assessment. ATSDR will base this determination on, among other factors:...

  9. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY... Act requests. The Office of Science and Technology Policy shall process requests for...

  10. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) A Appendix A to Part 2507 Public Welfare Regulations Relating to Public Welfare (Continued... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter (Sample... Information Act Request. Dear____: This is a request under the Freedom of Information Act. I request that...

  11. 6 CFR 1002.4 - Responses to Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Information Act procedures at 6 CFR part 1001. ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Responses to Privacy Act requests. 1002.4 Section 1002.4 Domestic Security PRIVACY AND CIVIL LIBERTIES OVERSIGHT BOARD IMPLEMENTATION OF THE...

  12. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Freedom of Information Act (FOIA) requests... Fishery Management Councils § 600.155 Freedom of Information Act (FOIA) requests. (a) FOIA requests... will forward the request to the NMFS FOIA Official to secure a FOIA number and log into the FOIA...

  13. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false How do I make a Privacy Act request? 1204.3 Section 1204.3 Banks and Banking FEDERAL HOUSING FINANCE AGENCY ORGANIZATION AND OPERATIONS PRIVACY ACT IMPLEMENTATION § 1204.3 How do I make a Privacy Act request? (a) What is a valid request? In general, a Privacy Act request can be made on your...

  14. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 9 2013-01-01 2013-01-01 false How do I make a Privacy Act request? 1204.3 Section 1204.3 Banks and Banking FEDERAL HOUSING FINANCE AGENCY ORGANIZATION AND OPERATIONS PRIVACY ACT IMPLEMENTATION § 1204.3 How do I make a Privacy Act request? (a) What is a valid request? In general, a Privacy Act request can be made on your...

  15. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... and envelope or any fax cover sheet should be clearly marked “Privacy Act Request.” A Privacy Act... mail or by fax, or delivered in person, a Privacy Act request will not be considered to have been... or name, author, recipient, and subject matter. (c) Proof of identity. Requests received by mail,...

  16. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... and envelope or any fax cover sheet should be clearly marked “Privacy Act Request.” A Privacy Act... mail or by fax, or delivered in person, a Privacy Act request will not be considered to have been... or name, author, recipient, and subject matter. (c) Proof of identity. Requests received by mail,...

  17. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and envelope or any fax cover sheet should be clearly marked “Privacy Act Request.” A Privacy Act... mail or by fax, or delivered in person, a Privacy Act request will not be considered to have been... or name, author, recipient, and subject matter. (c) Proof of identity. Requests received by mail,...

  18. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 33 2011-07-01 2011-07-01 false Making a Freedom of Information Act request. 1515.5 Section 1515.5 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY FREEDOM OF INFORMATION ACT PROCEDURES Procedures for Requesting Records § 1515.5 Making a Freedom of Information Act request. (a) Availability of records....

  19. 28 CFR 513.61 - Freedom of Information Act requests by inmates.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Freedom of Information Act requests by... MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.61 Freedom of Information Act requests by inmates. (a) Inmates are encouraged...

  20. 28 CFR 513.62 - Freedom of Information Act requests by former inmates.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Freedom of Information Act requests by... GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.62 Freedom of Information Act requests by former inmates. Former...

  1. 28 CFR 513.62 - Freedom of Information Act requests by former inmates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Freedom of Information Act requests by... GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.62 Freedom of Information Act requests by former inmates. Former...

  2. 28 CFR 513.61 - Freedom of Information Act requests by inmates.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Freedom of Information Act requests by... MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.61 Freedom of Information Act requests by inmates. (a) Inmates are encouraged...

  3. 28 CFR 513.61 - Freedom of Information Act requests by inmates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Freedom of Information Act requests by... MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.61 Freedom of Information Act requests by inmates. (a) Inmates are encouraged...

  4. 28 CFR 513.62 - Freedom of Information Act requests by former inmates.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Freedom of Information Act requests by... GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.62 Freedom of Information Act requests by former inmates. Former...

  5. 78 FR 62629 - Proposed Information Collection Request; Comment Request; Clean Water Act Section 404 State...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ...The Environmental Protection Agency is planning to submit an information collection request (ICR), ``Clean Water Act Section 404 State-Assumed Programs'' (EPA ICR No. 0220.12, OMB Control No. 2040- 0168) to the Office of Management and Budget (OMB) for review and approval in accordance with the Paperwork Reduction Act (44 U.S.C. 3501 et seq.). Before doing so, EPA is soliciting public comments......

  6. DNA analysis and the Freedom of Information Act: information or invasion?

    PubMed

    Might, C

    2001-01-01

    This note explores the possibility of release of an individual's DNA analysis to any person who requests it through the Freedom of Information Act (FOIA), after an individual's post-aircraft accident DNA profile has been developed by the Federal Aviation Administration's (FAA) Civil Aerospace Medical Institute (CAMI). It analyzes whether the request would fall under the FOIA's 552(b)(6) exemption, which weighs a person's privacy interest against any public interest in such information, or if the release would constitute a "clearly unwarranted invasion of personal privacy." PMID:12741386

  7. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Freedom of Information Act (FOIA... Fishery Management Councils § 600.155 Freedom of Information Act (FOIA) requests. (a) FOIA requests... will forward the request to the NMFS FOIA Official to secure a FOIA number and log into the FOIA...

  8. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Freedom of Information Act (FOIA... Fishery Management Councils § 600.155 Freedom of Information Act (FOIA) requests. (a) FOIA requests... will forward the request to the NMFS FOIA Official to secure a FOIA number and log into the FOIA...

  9. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false Freedom of Information Act (FOIA... Fishery Management Councils § 600.155 Freedom of Information Act (FOIA) requests. (a) FOIA requests... will forward the request to the NMFS FOIA Official to secure a FOIA number and log into the FOIA...

  10. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Freedom of Information Act (FOIA... Fishery Management Councils § 600.155 Freedom of Information Act (FOIA) requests. (a) FOIA requests... will forward the request to the NMFS FOIA Official to secure a FOIA number and log into the FOIA...

  11. 7 CFR 1700.3 - Requests under the Freedom of Information Act.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Information Act are found at 7 CFR part 1. Requests must be in writing and may be submitted in person or by... CFR 1.16, fees may be charged for processing of requests for records. An appeal of the agency... 7 Agriculture 11 2013-01-01 2013-01-01 false Requests under the Freedom of Information Act....

  12. 7 CFR 1700.3 - Requests under the Freedom of Information Act.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Information Act are found at 7 CFR part 1. Requests must be in writing and may be submitted in person or by... CFR 1.16, fees may be charged for processing of requests for records. An appeal of the agency... 7 Agriculture 11 2014-01-01 2014-01-01 false Requests under the Freedom of Information Act....

  13. 78 FR 32227 - Perishable Agricultural Commodities Act; Request for Extension and Revision of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-29

    ... Agricultural Marketing Service Perishable Agricultural Commodities Act; Request for Extension and Revision of a Currently Approved Information Collection AGENCY: Agricultural Marketing Service, USDA. ACTION: Notice and... 35), this document announces the Agricultural Marketing Service's (AMS) intention to request...

  14. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Freedom of Information Act Request Letter (Sample) A Appendix A to Part 2507 Public Welfare Regulations Relating to Public Welfare (Continued... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter...

  15. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Freedom of Information Act Request Letter (Sample) A Appendix A to Part 2507 Public Welfare Regulations Relating to Public Welfare (Continued... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter...

  16. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Freedom of Information Act Request Letter (Sample) A Appendix A to Part 2507 Public Welfare Regulations Relating to Public Welfare (Continued... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter...

  17. 12 CFR 1204.4 - How will FHFA respond to my Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false How will FHFA respond to my Privacy Act request? 1204.4 Section 1204.4 Banks and Banking FEDERAL HOUSING FINANCE AGENCY ORGANIZATION AND OPERATIONS PRIVACY ACT IMPLEMENTATION § 1204.4 How will FHFA respond to my Privacy Act request? (a) How will FHFA locate the requested records? FHFA will...

  18. 12 CFR 1204.4 - How will FHFA respond to my Privacy Act request?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 7 2011-01-01 2011-01-01 false How will FHFA respond to my Privacy Act request? 1204.4 Section 1204.4 Banks and Banking FEDERAL HOUSING FINANCE AGENCY ORGANIZATION AND OPERATIONS PRIVACY ACT IMPLEMENTATION § 1204.4 How will FHFA respond to my Privacy Act request? (a) How will FHFA locate the requested records? FHFA will...

  19. 75 FR 70160 - Affordable Care Act; Federal External Review Process; Request for Information

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... provisions of PHS Act section 2719 on July 23, 2010, at 75 FR 43330. Section 2719(b)(1) of the PHS Act and... Administration 29 CFR Part 2590 Affordable Care Act; Federal External Review Process; Request for Information... Act, as amended by the Affordable Care Act, and its implementing regulations. DATES: Submit written...

  20. The Balancing Act of Ribonucleotides in DNA.

    PubMed

    Cerritelli, Susana M; Crouch, Robert J

    2016-05-01

    The abundance of ribonucleotides in DNA remained undetected until recently because they are efficiently removed by the ribonucleotide excision repair (RER) pathway, a process similar to Okazaki fragment (OF) processing after incision by Ribonuclease H2 (RNase H2). All DNA polymerases incorporate ribonucleotides during DNA synthesis. How many, when, and why they are incorporated has been the focus of intense work during recent years by many labs. In this review, we discuss recent advances in ribonucleotide incorporation by eukaryotic DNA polymerases that suggest an evolutionarily conserved role for ribonucleotides in DNA. We also review the data that indicate that removal of ribonucleotides has an important role in maintaining genome stability. PMID:26996833

  1. 76 FR 9611 - Notice of Permit Modification Request Received Under the Antarctic Conservation Act of 1978

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-18

    ... Notice of Permit Modification Request Received Under the Antarctic Conservation Act of 1978 AGENCY: National Science Foundation. ACTION: Notice of permit modification request received under the Antarctic... modification by March 21, 2011. The permit modification request may be inspected by interested parties at...

  2. 46 CFR 503.31 - Records available upon written request under the Freedom of Information Act.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 9 2014-10-01 2014-10-01 false Records available upon written request under the Freedom... ADMINISTRATIVE PROVISIONS PUBLIC INFORMATION Requests for Records Under the Freedom of Information § 503.31 Records available upon written request under the Freedom of Information Act. (a) A member of the...

  3. 46 CFR 503.31 - Records available upon written request under the Freedom of Information Act.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 9 2013-10-01 2013-10-01 false Records available upon written request under the Freedom... ADMINISTRATIVE PROVISIONS PUBLIC INFORMATION Requests for Records Under the Freedom of Information § 503.31 Records available upon written request under the Freedom of Information Act. (a) A member of the...

  4. 46 CFR 503.31 - Records available upon written request under the Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Records available upon written request under the Freedom... ADMINISTRATIVE PROVISIONS PUBLIC INFORMATION Requests for Records Under the Freedom of Information § 503.31 Records available upon written request under the Freedom of Information Act. (a) A member of the...

  5. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Maintenance of Meeting Records § 1703.403 Requests for records under Freedom of Information and Privacy Acts... 45 Public Welfare 4 2011-10-01 2011-10-01 false Requests for records under Freedom of...

  6. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Maintenance of Meeting Records § 1703.403 Requests for records under Freedom of Information and Privacy Acts... 45 Public Welfare 4 2010-10-01 2010-10-01 false Requests for records under Freedom of...

  7. Effects of Culture and Gender in Comprehension of Speech Acts of Indirect Request

    ERIC Educational Resources Information Center

    Shams, Rabe'a; Afghari, Akbar

    2011-01-01

    This study investigates the comprehension of indirect request speech act used by Iranian people in daily communication. The study is an attempt to find out whether different cultural backgrounds and the gender of the speakers affect the comprehension of the indirect request of speech act. The sample includes thirty males and females in Gachsaran…

  8. 77 FR 53237 - Submission for Review; Information Collection: Freedom of Information/Privacy Act Record Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-31

    ... MANAGEMENT Submission for Review; Information Collection: Freedom of Information/Privacy Act Record Request... request (ICR), Office of Management and Budget (OMB) Control No. 3206-NEW, Freedom of Information/Privacy... Federal Investigative Services (FIS), Freedom of Information and Privacy Act (FOI/PA) office proposes...

  9. 28 CFR 513.64 - Acknowledgment of Freedom of Information Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Acknowledgment of Freedom of Information... GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.64 Acknowledgment of Freedom of Information Act requests. (a) All...

  10. 40 CFR 1515.5 - How to make a Freedom of Information Act request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false How to make a Freedom of Information Act request. 1515.5 Section 1515.5 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY FREEDOM OF INFORMATION ACT PROCEDURES Procedures for Requesting Records § 1515.5 How to make a Freedom...

  11. 29 CFR 1209.09 - Requests for records under Freedom of Information Act.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 4 2014-07-01 2014-07-01 false Requests for records under Freedom of Information Act. 1209... OBSERVATION OF NATIONAL MEDIATION BOARD MEETINGS § 1209.09 Requests for records under Freedom of Information... under this part may be pursued in accordance with the Freedom of Information Act (5 U.S.C. 552)....

  12. 28 CFR 513.64 - Acknowledgment of Freedom of Information Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Acknowledgment of Freedom of Information... GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.64 Acknowledgment of Freedom of Information Act requests. (a) All...

  13. 29 CFR 1209.09 - Requests for records under Freedom of Information Act.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 4 2013-07-01 2013-07-01 false Requests for records under Freedom of Information Act. 1209... OBSERVATION OF NATIONAL MEDIATION BOARD MEETINGS § 1209.09 Requests for records under Freedom of Information... under this part may be pursued in accordance with the Freedom of Information Act (5 U.S.C. 552)....

  14. 28 CFR 513.64 - Acknowledgment of Freedom of Information Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Acknowledgment of Freedom of Information... GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.64 Acknowledgment of Freedom of Information Act requests. (a) All...

  15. 78 FR 23885 - Federal Seed Act Program; Request for Extension and Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-23

    ... Agricultural Marketing Service Federal Seed Act Program; Request for Extension and Revision of a Currently Approved Information Collection AGENCY: Agricultural Marketing Service, USDA. ACTION: Request for comments... announces the Agricultural Marketing Service's (AMS) intention to request approval, from the Office...

  16. 38 CFR 20.1200 - Rule 1200. Privacy Act request-appeal pending.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Rule 1200. Privacy Act... VETERANS AFFAIRS (CONTINUED) BOARD OF VETERANS' APPEALS: RULES OF PRACTICE Privacy Act § 20.1200 Rule 1200. Privacy Act request—appeal pending. When a Privacy Act request is filed under § 1.577 of this chapter...

  17. 38 CFR 20.1200 - Rule 1200. Privacy Act request-appeal pending.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2013-07-01 2013-07-01 false Rule 1200. Privacy Act... VETERANS AFFAIRS (CONTINUED) BOARD OF VETERANS' APPEALS: RULES OF PRACTICE Privacy Act § 20.1200 Rule 1200. Privacy Act request—appeal pending. When a Privacy Act request is filed under § 1.577 of this chapter...

  18. 38 CFR 20.1200 - Rule 1200. Privacy Act request-appeal pending.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Rule 1200. Privacy Act... VETERANS AFFAIRS (CONTINUED) BOARD OF VETERANS' APPEALS: RULES OF PRACTICE Privacy Act § 20.1200 Rule 1200. Privacy Act request—appeal pending. When a Privacy Act request is filed under § 1.577 of this chapter...

  19. 38 CFR 20.1200 - Rule 1200. Privacy Act request-appeal pending.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2014-07-01 2014-07-01 false Rule 1200. Privacy Act... VETERANS AFFAIRS (CONTINUED) BOARD OF VETERANS' APPEALS: RULES OF PRACTICE Privacy Act § 20.1200 Rule 1200. Privacy Act request—appeal pending. When a Privacy Act request is filed under § 1.577 of this chapter...

  20. 7 CFR 1700.3 - Requests under the Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Information Act are found at 7 CFR part 1. Requests must be in writing and may be submitted in person or by... Independence Avenue, SW., STOP 1530, Washington, DC 20250-1530; or by FAX to 202-401-1977. As set forth in 7 CFR 1.16, fees may be charged for processing of requests for records. An appeal of the...

  1. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....gov/administration/eop/ceq, and an online Freedom of Information Act Requester Service Center (“Center... information regarding the CEQ's FOIA Public Liaison, as defined in Section 1515.4(b), and access CEQ's Online... matter, are likely to be the subject of FOIA requests. To save both time and money, CEQ strongly...

  2. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....gov/administration/eop/ceq, and an online Freedom of Information Act Requester Service Center (“Center... information regarding the CEQ's FOIA Public Liaison, as defined in Section 1515.4(b), and access CEQ's Online... matter, are likely to be the subject of FOIA requests. To save both time and money, CEQ strongly...

  3. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....gov/administration/eop/ceq, and an online Freedom of Information Act Requester Service Center (“Center... information regarding the CEQ's FOIA Public Liaison, as defined in Section 1515.4(b), and access CEQ's Online... matter, are likely to be the subject of FOIA requests. To save both time and money, CEQ strongly...

  4. 77 FR 72359 - Animal Generic Drug User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... HUMAN SERVICES Food and Drug Administration Animal Generic Drug User Fee Act; Public Meeting; Request for Comments AGENCY: Food and Drug Administration, HHS. ACTION: Notice of meeting; request for comments. The Food and Drug Administration (FDA) is announcing the following meeting: Animal Generic...

  5. 77 FR 4564 - Request for Information Regarding the Reinsurance Program Under the Affordable Care Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-30

    ..., 2011 (76 FR 41930). The Affordable Care Act instructs each State to establish or contract with an.... ACTION: Request for information. SUMMARY: This notice is a request for information (RFI) to gain market information on entities that could administer a transitional reinsurance program. This RFI will inform one...

  6. 29 CFR 1209.09 - Requests for records under Freedom of Information Act.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 4 2011-07-01 2011-07-01 false Requests for records under Freedom of Information Act. 1209.09 Section 1209.09 Labor Regulations Relating to Labor (Continued) NATIONAL MEDIATION BOARD PUBLIC OBSERVATION OF NATIONAL MEDIATION BOARD MEETINGS § 1209.09 Requests for records under Freedom of...

  7. 29 CFR 1209.09 - Requests for records under Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Requests for records under Freedom of Information Act. 1209.09 Section 1209.09 Labor Regulations Relating to Labor (Continued) NATIONAL MEDIATION BOARD PUBLIC OBSERVATION OF NATIONAL MEDIATION BOARD MEETINGS § 1209.09 Requests for records under Freedom of...

  8. 78 FR 24227 - Notice of Proposed Information Collection; Comment Request: Real Estate Settlement Procedures Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection; Comment Request: Real Estate Settlement... information: Title of Proposal: Real Estate Settlement Procedures Act (RESPA) Disclosures. OMB Control Number... 24 CFR part 3500, require real estate settlement service providers to give homebuyers...

  9. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... declassification that are submitted under the provisions of the Freedom of Information Act, as amended, (See § 0.461), of the Privacy Act of 1974, (See § 0.554) shall be processed in accordance with the...

  10. Requests Made under the Official Information Act 1982: A Survey at the Agency Level.

    ERIC Educational Resources Information Center

    Clemens, Dave Andrew

    New Zealand's Official Information Act (OIA) has been in force for almost two decades. During these years, there has been little research published about how citizens use the legislation or how agencies have responded to requests made under the Act. The aim of this project is to produce basic research data from a range of agencies to quantify use…

  11. 77 FR 72356 - Animal Drug User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... HUMAN SERVICES Food and Drug Administration Animal Drug User Fee Act; Public Meeting; Request for... Food and Drug Administration (FDA) is announcing the following meeting: Animal Drug User Fee Act. The topic to be discussed is proposed recommendations for the reauthorization of the Animal Drug User...

  12. 76 FR 18254 - Proposed Information Collection Request (ICR) for the Family Medical Leave Act (FMLA) Employee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... Proposed Information Collection Request (ICR) for the Family Medical Leave Act (FMLA) Employee and Employer... assessed. The Department is soliciting comments concerning its proposal to collect information on employees... conditions and the Family and Medical Leave Act (FMLA) regulations since the 2000 employee and...

  13. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Requests for records under Freedom of Information and Privacy Acts. 1703.403 Section 1703.403 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Maintenance of Meeting Records § 1703.403...

  14. 28 CFR 513.66 - Denials and appeals of Freedom of Information Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Denials and appeals of Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.66 Denials and appeals of Freedom of Information Act...

  15. 28 CFR 513.65 - Review of documents for Freedom of Information Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Review of documents for Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.65 Review of documents for Freedom of Information Act...

  16. 28 CFR 513.66 - Denials and appeals of Freedom of Information Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Denials and appeals of Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.66 Denials and appeals of Freedom of Information Act...

  17. 28 CFR 513.65 - Review of documents for Freedom of Information Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Review of documents for Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.65 Review of documents for Freedom of Information Act...

  18. 28 CFR 513.65 - Review of documents for Freedom of Information Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Review of documents for Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.65 Review of documents for Freedom of Information Act...

  19. 28 CFR 513.66 - Denials and appeals of Freedom of Information Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Denials and appeals of Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act Requests for Information § 513.66 Denials and appeals of Freedom of Information Act...

  20. Requests for euthanasia in general practice before and after implementation of the Dutch Euthanasia Act

    PubMed Central

    van Alphen, Jojanneke E; Donker, Gé A; Marquet, Richard L

    2010-01-01

    Background The Netherlands was the first country in the world to implement a Euthanasia Act in 2002. It is unknown whether legalising euthanasia under strict conditions influences the number and nature of euthanasia requests. Aim To investigate changes in the number of, and reasons for, requests for euthanasia in Dutch general practice after implementation of the Euthanasia Act. Design of study Retrospective dynamic cohort study comparing 5 years before (1998–2002) and 5 years after (2003–2007) implementation of the Act. Method Standardised registration forms were used to collect data on requests for euthanasia via the Dutch Sentinel Practice Network. This network of 45 general practices is nationally representative by age, sex, geographic distribution, and population density. Results The mean annual incidence of requests before implementation amounted to 3.1/10 000 and thereafter to 2.8/10 000 patients. However, trends differed by sex. The number of requests by males decreased significantly from 3.7/10 000 to 2.6/10 000 (P = 0.008); the requests by females increased non-significantly from 2.6/10 000 to 3.1/10 000. Before and after implementation, cancer remained the major underlying disease for requesting euthanasia: 82% versus 77% for men; 73% versus 75% for females. Pain was a major reason for a request, increasing in the period before implementation (mean 27%), but declining in the period thereafter (mean 22%). Loss of dignity became a less important reason after implementation (from 18% to 10%, P = 0.04), predominantly due to a marked decrease in the number of females citing it as a reason (from 17% to 6%, P = 0.02). Conclusion There was no increase in demand for euthanasia after implementation of the Euthanasia Act. Pain as a reason for requesting euthanasia showed an increasing trend before implementation, but declined thereafter. Loss of dignity as a reason declined, especially in females. PMID:20353671

  1. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Freedom of Information Act and Privacy Act... INFORMATION SECURITY PROGRAM Declassification and Downgrading § 2400.22 Freedom of Information Act and Privacy... that are submitted under the provisions of the Freedom of Information Act, as amended, or the...

  2. 76 FR 58277 - Animal Generic Drug User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Animal Generic Drug User Fee Act; Public Meeting; Request... meeting participants better understand the history and evolution of AGDUFA, and its current status....

  3. 75 FR 49502 - Medical Device User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration Medical Device User Fee Act; Public Meeting; Request for... provided to help potential meeting participants better understand the history and evolution of the...

  4. Interlanguage Pragmatics in Russian: The Speech Act of Request in Email

    ERIC Educational Resources Information Center

    Krulatz, Anna M.

    2012-01-01

    As face-threatening speech acts, requests are of particular interest to second language acquisition scholars. They affect the interlocutors' public self-images, and thus require a careful consideration of the social distance between the interlocutors, their status, and the level of the imposition, factors that are weighed differently in…

  5. Pragmatic Production of Third Language Learners of English: A Focus on Request Acts Modifiers

    ERIC Educational Resources Information Center

    Jorda, Maria Pilar Safont

    2005-01-01

    The present study examines pragmatic production of monolingual (L1 Castilian) and bilingual (L1 Catalan, L2 Castilian) learners of English in a foreign language learning context, that of the Valencian Community in Spain. We particularly focused on proficiency-level effects and on the role of the task type in the use of request acts peripheral…

  6. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Requests for records under Freedom of Information and Privacy Acts. 1703.403 Section 1703.403 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE...

  7. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Requests for records under Freedom of Information and Privacy Acts. 1703.403 Section 1703.403 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE...

  8. 75 FR 996 - Medicare Prescription Drug, Improvement, and Modernization Act of 2003 Section 1013: Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-07

    ... Modernization Act of 2003 Section 1013: Request for Nominations--The Effective Health Care Stakeholder Group... nominations for the Effective Health Care Stakeholder Group. SUMMARY: The DHHS Agency for Healthcare Research... members of the Stakeholder Group to support the work of the Effective Health Care Program,...

  9. 77 FR 24555 - Andean Trade Preference Act (ATPA), as Amended: Request for Public Comments Regarding Beneficiary...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-24

    ... Register notice published on August 15, 2002 (67 FR 53379), for a full list of the eligibility criteria... TRADE REPRESENTATIVE Andean Trade Preference Act (ATPA), as Amended: Request for Public Comments Regarding Beneficiary Countries AGENCY: Office of the United States Trade Representative. ACTION:...

  10. 75 FR 19669 - Andean Trade Preference Act (ATPA), as Amended: Request for Public Comments Regarding Beneficiary...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ... August 15, 2002 (67 FR 53379), for a full list of the eligibility criteria. 2. Requirements for... TRADE REPRESENTATIVE Andean Trade Preference Act (ATPA), as Amended: Request for Public Comments Regarding Beneficiary Countries AGENCY: Office of the United States Trade Representative. ACTION:...

  11. 76 FR 40723 - Agency Information Collection Activities; Proposed Collection; Comment Request; Clean Water Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... grants to States for the establishment of State Water Pollution Control Revolving Funds (SRF). The... (CWA). The 1987 Act declares that water pollution control revolving funds shall be administered by an... AGENCY Agency Information Collection Activities; Proposed Collection; Comment Request; Clean Water...

  12. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Freedom of Information Act and Privacy Act... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY INFORMATION SECURITY PROGRAM Declassification and Downgrading § 2400.22 Freedom of Information Act and...

  13. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Freedom of Information Act and Privacy Act... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY INFORMATION SECURITY PROGRAM Declassification and Downgrading § 2400.22 Freedom of Information Act and...

  14. 46 CFR 503.32 - Procedures for responding to requests made under the Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Freedom of Information Act. 503.32 Section 503.32 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS PUBLIC INFORMATION Requests for Records Under the Freedom of Information § 503.32 Procedures for responding to requests made under the Freedom of Information Act. (a) Determination to...

  15. 46 CFR 503.32 - Procedures for responding to requests made under the Freedom of Information Act.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Freedom of Information Act. 503.32 Section 503.32 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS PUBLIC INFORMATION Requests for Records Under the Freedom of Information § 503.32 Procedures for responding to requests made under the Freedom of Information Act. (a) Determination to...

  16. 46 CFR 503.32 - Procedures for responding to requests made under the Freedom of Information Act.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Freedom of Information Act. 503.32 Section 503.32 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS PUBLIC INFORMATION Requests for Records Under the Freedom of Information § 503.32 Procedures for responding to requests made under the Freedom of Information Act. (a) Determination to...

  17. 12 CFR 1204.5 - What if I am dissatisfied with the FHFA response to my Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false What if I am dissatisfied with the FHFA response to my Privacy Act request? 1204.5 Section 1204.5 Banks and Banking FEDERAL HOUSING FINANCE AGENCY ORGANIZATION AND OPERATIONS PRIVACY ACT IMPLEMENTATION § 1204.5 What if I am dissatisfied with the FHFA response to my Privacy Act request? (a) May...

  18. 12 CFR 1204.5 - What if I am dissatisfied with the FHFA response to my Privacy Act request?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 7 2011-01-01 2011-01-01 false What if I am dissatisfied with the FHFA response to my Privacy Act request? 1204.5 Section 1204.5 Banks and Banking FEDERAL HOUSING FINANCE AGENCY ORGANIZATION AND OPERATIONS PRIVACY ACT IMPLEMENTATION § 1204.5 What if I am dissatisfied with the FHFA response to my Privacy Act request? (a) May...

  19. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  20. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  1. 3 CFR - State of California Request for Waiver Under 42 U.S.C. 7543(b), the Clean Air Act

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....C. 7543(b), the Clean Air Act Presidential Documents Other Presidential Documents Memorandum of January 26, 2009 State of California Request for Waiver Under 42 U.S.C. 7543(b), the Clean Air Act Memorandum for the Administrator of the Environmental Protection Agency Under the Clean Air Act (42...

  2. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE TRIBAL CLEAN AIR ACT AUTHORITY Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act program...

  3. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  4. 75 FR 19297 - Medical Loss Ratios; Request for Comments Regarding Section 2718 of the Public Health Service Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... at 64 FR 70164, December 15, 1999. B. Public Reporting of the Ratio of Incurred Claims to Earned... Medical Loss Ratios; Request for Comments Regarding Section 2718 of the Public Health Service Act AGENCY... information. ] SUMMARY: This document is a request for comments regarding Section 2718 of the Public...

  5. 78 FR 15019 - Food and Drug Administration Prescription Drug User Fee Act V Benefit-Risk Plan; Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... HUMAN SERVICES Food and Drug Administration Food and Drug Administration Prescription Drug User Fee Act V Benefit-Risk Plan; Request for Comments AGENCY: Food and Drug Administration, HHS. ACTION: Notice, request for comments. SUMMARY: The Food and Drug Administration (FDA or the Agency) is announcing...

  6. 77 FR 33388 - Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-06

    ... Animal and Plant Health Inspection Service Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration Requirement; Plants and Plant Products AGENCY: Animal and Plant Health... Plant Health Inspection Service's intention to request an extension of approval of an...

  7. 17 CFR Appendix A to Part 146 - Fees for Copies of Records Requested Under the Privacy Act of 1974

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 1 2011-04-01 2011-04-01 false Fees for Copies of Records Requested Under the Privacy Act of 1974 A Appendix A to Part 146 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION RECORDS MAINTAINED ON INDIVIDUALS Pt. 146, App. A Appendix A to Part 146—Fees for Copies of Records Requested...

  8. 28 CFR 513.63 - Freedom of Information Act requests on behalf of an inmate or former inmate.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) months of the date of the request letter. Identification data, as listed in 28 CFR 16.41, must be... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Freedom of Information Act requests on..., DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information...

  9. 28 CFR 513.63 - Freedom of Information Act requests on behalf of an inmate or former inmate.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) months of the date of the request letter. Identification data, as listed in 28 CFR 16.41, must be... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Freedom of Information Act requests on..., DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information...

  10. 28 CFR 513.63 - Freedom of Information Act requests on behalf of an inmate or former inmate.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) months of the date of the request letter. Identification data, as listed in 28 CFR 16.41, must be... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Freedom of Information Act requests on..., DEPARTMENT OF JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information...

  11. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... TRIBAL CLEAN AIR ACT AUTHORITY Tribal Authority § 49.5 Tribal requests for additional Clean Air Act... request that the Administrator specify additional provisions of the Clean Air Act for which it would be... Air Act provisions for which it is not appropriate to treat tribes in the same manner as States....

  12. 15 CFR Appendix B to Part 4 - Officials Authorized To Deny Requests for Records Under the Freedom of Information Act, and...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Information Technologies Director, Office of Telecommunications Technologies U.S. and Foreign Commercial... Secretary for Information Technology Industries Deputy Assistant Secretary for Environmental Technologies... for Records Under the Freedom of Information Act, and Requests for Records and Requests for...

  13. The Effect of Explicit Metapragmatic Instruction on Request Speech Act Awareness of Intermediate EFL Students at Institute Level

    ERIC Educational Resources Information Center

    Masouleh, Fatemeh Abdollahizadeh; Arjmandi, Masoumeh; Vahdany, Fereydoon

    2014-01-01

    This study deals with the application of the pragmatics research to EFL teaching. The need for language learners to utilize a form of speech acts such as request which involves a series of strategies was significance of the study. Although defining different speech acts has been established since 1960s, recently there has been a shift towards…

  14. 8 CFR 103.10 - Requests for records under the Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., district offices, and sub-offices see 8 CFR 100.4. (2) Manner of requesting records. All Freedom of... under 28 CFR 16.1(d). A request improperly addressed will not be deemed to have been received for..., requesters may deem their request to be denied and exercise their right to appeal in accordance with 28...

  15. 8 CFR 103.10 - Requests for records under the Freedom of Information Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., district offices, and sub-offices see 8 CFR 100.4. (2) Manner of requesting records. All Freedom of... under 28 CFR 16.1(d). A request improperly addressed will not be deemed to have been received for..., requesters may deem their request to be denied and exercise their right to appeal in accordance with 28...

  16. 75 FR 60065 - Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ...; ] DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration Requirement; Plants and Plant Products AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Extension of approval of an...

  17. Examination of Learner and Situation Level Variables: Choice of Speech Act and Request Strategy by Spanish L2 Learners

    ERIC Educational Resources Information Center

    Kuriscak, Lisa

    2015-01-01

    This study focuses on variation within a group of learners of Spanish (N = 253) who produced requests and complaints via a written discourse completion task. It examines the effects of learner and situational variables on production--the effect of proficiency and addressee-gender on speech-act choice and the effect of perception of imposition on…

  18. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Request by an Indian tribe for eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE INDIAN COUNTRY: AIR QUALITY PLANNING AND MANAGEMENT Tribal Authority § 49.7...

  19. 36 CFR 1256.74 - How does NARA process Freedom of Information Act (FOIA) requests for classified information?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... accordance with the provisions of 36 CFR part 1250. Time limits for responses to FOIA requests for classified... FOIA, the PRA, and Executive Order 13233, Further Implementation of the Presidential Records Act (3 CFR... CFR, 1995 Comp., p. 333), as amended by Executive Order 13292 (68 FR 15315, 3 CFR, 2003 Comp., p....

  20. 36 CFR 1256.74 - How does NARA process Freedom of Information Act (FOIA) requests for classified information?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... accordance with the provisions of 36 CFR part 1250. Time limits for responses to FOIA requests for classified... FOIA, the PRA, and Executive Order 13233, Further Implementation of the Presidential Records Act (3 CFR... CFR, 1995 Comp., p. 333), as amended by Executive Order 13292 (68 FR 15315, 3 CFR, 2003 Comp., p....

  1. 36 CFR 1256.74 - How does NARA process Freedom of Information Act (FOIA) requests for classified information?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... accordance with the provisions of 36 CFR part 1250. Time limits for responses to FOIA requests for classified... FOIA, the PRA, and Executive Order 13233, Further Implementation of the Presidential Records Act (3 CFR... CFR, 1995 Comp., p. 333), as amended by Executive Order 13292 (68 FR 15315, 3 CFR, 2003 Comp., p....

  2. 76 FR 69792 - Notice of Exemption Request Under Section 312 of the Small Business Investment Act, Conflicts of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-09

    ... ADMINISTRATION Notice of Exemption Request Under Section 312 of the Small Business Investment Act, Conflicts of Interest; Contemporary Healthcare Senior Lien Fund I, LP Notice is hereby given that Contemporary Healthcare Senior Lien Fund I, LP, License No. 02/02-0649, 1040 Broad Street, Suite 103, Shrewsbury, NJ,...

  3. 28 CFR Appendix to Subpart H of... - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... by 28 CFR 16.5 (b) and (c) and 16.45(a), as amended July 1, 1977, and defined in those sections, for... Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H of Part 0 Judicial Administration DEPARTMENT OF JUSTICE ORGANIZATION OF THE DEPARTMENT OF JUSTICE Antitrust Division Pt. 0, Subpt....

  4. 28 CFR Appendix to Subpart H of... - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... by 28 CFR 16.5 (b) and (c) and 16.45(a), as amended July 1, 1977, and defined in those sections, for... Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H of Part 0 Judicial Administration DEPARTMENT OF JUSTICE ORGANIZATION OF THE DEPARTMENT OF JUSTICE Antitrust Division Pt. 0, Subpt....

  5. 28 CFR Appendix to Subpart H - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... duties and responsibilities previously assigned to the Assistant Attorney General by 28 CFR 16.5 (b) and... Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H Judicial Administration DEPARTMENT OF JUSTICE ORGANIZATION OF THE DEPARTMENT OF JUSTICE Antitrust Division Pt. 0, Subpt. H,...

  6. 17 CFR Appendix A to Part 146 - Fees for Copies of Records Requested Under the Privacy Act of 1974

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Fees for Copies of Records Requested Under the Privacy Act of 1974 A Appendix A to Part 146 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION RECORDS MAINTAINED ON INDIVIDUALS Pt. 146, App. A Appendix A to...

  7. 75 FR 35025 - Clean Air Act Advisory Committee (CAAAC); Request for Nominations for 2010 Clean Air Excellence...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... AGENCY Clean Air Act Advisory Committee (CAAAC); Request for Nominations for 2010 Clean Air Excellence... Air Excellence Awards. SUMMARY: EPA established the Clean Air Excellence Awards Program in February... progress in achieving clean air. This notice announces the competition for the Year 2010 program....

  8. 76 FR 40728 - Clean Air Act Advisory Committee (CAAAC); Request for Nominations for 2011 Clean Air Excellence...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... AGENCY Clean Air Act Advisory Committee (CAAAC); Request for Nominations for 2011 Clean Air Excellence... Air Excellence Awards. ] SUMMARY: EPA established the Clean Air Excellence Awards Program in February... progress in achieving clean air. This notice announces the competition for the Year 2011 program....

  9. 28 CFR Appendix to Subpart H - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... duties and responsibilities previously assigned to the Assistant Attorney General by 28 CFR 16.5 (b) and... Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H Judicial Administration DEPARTMENT OF JUSTICE ORGANIZATION OF THE DEPARTMENT OF JUSTICE Antitrust Division Pt. 0, Subpt. H,...

  10. 28 CFR Appendix to Subpart H - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... duties and responsibilities previously assigned to the Assistant Attorney General by 28 CFR 16.5 (b) and... Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H Judicial Administration DEPARTMENT OF JUSTICE ORGANIZATION OF THE DEPARTMENT OF JUSTICE Antitrust Division Pt. 0, Subpt. H,...

  11. 75 FR 15458 - Request for Small Reclamation Projects Act Loan To Construct Narrows Dam in Sanpete County, UT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... 25, 2003 (68 FR 66123-66124). The SDEIS describes and analyzes the potential effects of three action... Bureau of Reclamation Request for Small Reclamation Projects Act Loan To Construct Narrows Dam in Sanpete... law. These Reclamation actions would facilitate the construction by SWCD of the proposed Narrows...

  12. 36 CFR 1256.74 - How does NARA process Freedom of Information Act (FOIA) requests for classified information?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... accordance with the provisions of 36 CFR part 1250. Time limits for responses to FOIA requests for classified... CFR, 1995 Comp., p. 333), as amended by Executive Order 13292 (68 FR 15315, 3 CFR, 2003 Comp., p. 196... FOIA, the PRA, and Executive Order 13233, Further Implementation of the Presidential Records Act (3...

  13. [Cu(phen)2](2+) acts as electrochemical indicator and anchor to immobilize probe DNA in electrochemical DNA biosensor.

    PubMed

    Yang, Linlin; Li, Xiaoyu; Li, Xi; Yan, Songling; Ren, Yinna; Wang, Mengmeng; Liu, Peng; Dong, Yulin; Zhang, Chaocan

    2016-01-01

    We demonstrate a novel protocol for sensitive in situ label-free electrochemical detection of DNA hybridization based on copper complex ([Cu(phen)2](2+), where phen = 1,10-phenanthroline) and graphene (GR) modified glassy carbon electrode. Here, [Cu(phen)2](2+) acted advantageously as both the electrochemical indicator and the anchor for probe DNA immobilization via intercalative interactions between the partial double helix structure of probe DNA and the vertical aromatic groups of phen. GR provided large density of docking site for probe DNA immobilization and increased the electrical conductivity ability of the electrode. The modification procedure was monitored by electrochemical impedance spectroscopy (EIS). Square-wave voltammetry (SWV) was used to explore the hybridization events. Under the optimal conditions, the designed electrochemical DNA biosensor could effectively distinguish different mismatch degrees of complementary DNA from one-base mismatch to noncomplementary, indicating that the biosensor had high selectivity. It also exhibited a reasonable linear relationship. The oxidation peak currents of [Cu(phen)2](2+) were linear with the logarithm of the concentrations of complementary target DNA ranging from 1 × 10(-12) to 1 × 10(-6) M with a detection limit of 1.99 × 10(-13) M (signal/noise = 3). Moreover, the stability of the electrochemical DNA biosensor was also studied. PMID:26403602

  14. 76 FR 32991 - Request (ICR) for the Family Medical Leave Act (FMLA) Employee and Employer Surveys; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Wage and...; Comment Request AGENCY: Wage and Hour Division, Department of Labor. ACTION: Notice and Extension of... of collection requirements on respondents can be properly assessed. The Wage and Hour Division...

  15. DNA acts as a nucleation site for transient cavitation in the ultrasonic nebulizer.

    PubMed

    Lentz, Y K; Anchordoquy, T J; Lengsfeld, C S

    2006-03-01

    Several new technologies based upon ultrasound technology have been proposed as a method to enhance the delivery of genetic therapeutics. Using ultrasonic nebulization and a well-established method to quantitatively monitor transient cavitation, this study investigates the extent and factors influencing the degradation of DNA. Results from our studies show that the presence of DNA greatly enhances cavitation, and that the number of transient cavitation events also increases with the hydrodynamic diameter and number of DNA molecules in solution. More importantly, removing saturated gases from the plasmid DNA solutions resulted in a decrease in transient cavitation events but not degradation rate, suggesting that the cavitation event responsible for degradation occurs locally at the DNA molecule. Finally, complexing plasmid DNA with the cationic polymer polyethylenimine protected the native structure by reducing the molecule's potential to act as a heterogeneous nucleation site for transient cavitation. PMID:16432878

  16. DNA Identification Act of 1993. House of Representatives, One Hundred Third Congress, First Session

    SciTech Connect

    Not Available

    1993-01-01

    Report 103-45 on the DNA Identification Act of 1993 is intended to amend H.R. 829 (to amend title I of the Omnibus Crime Control and Safe Streets Act of 1968 to authorize funds received by States and units of the local government to be expended to improve the quality and availability of DNA records; to authorize the establishment of a DNA identification index; and for other purposes). The report states that the Committee on the Judiciary recommends Bill H.R. 829 pass with the amendment contained in this report. This report explains the amendment, the committee vote on H.R. 829, background behind H.R. 829, quality control concerns, proficincy testing, the significance of DNA matches, and databanking and privacy. Also included is a section-by-section analysis of H.R. 829 and committee oversight findings.

  17. 75 FR 32483 - Prescription Drug User Fee Act; Meetings on Reauthorization; Request for Notification of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ... reauthorization (75 FR 12555, March 16, 2010). This meeting and written comments submitted to the docket have...; Request for Notification of Stakeholder Intention to Participate AGENCY: Food and Drug Administration, HHS...) is issuing this notice to request that public stakeholders--including patient and consumer...

  18. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air Act... Air Act provisions for which it is not appropriate to treat tribes in the same manner as States....

  19. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air Act... Air Act provisions for which it is not appropriate to treat tribes in the same manner as States....

  20. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air Act... Air Act provisions for which it is not appropriate to treat tribes in the same manner as States....

  1. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air Act... Air Act provisions for which it is not appropriate to treat tribes in the same manner as States....

  2. 43 CFR 2.27 - How will a bureau handle a request for information that is contained in a Privacy Act system of...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false How will a bureau handle a request for information that is contained in a Privacy Act system of records? (See DOI's Privacy Act regulations (Subpart... system of records? (See DOI's Privacy Act regulations (Subpart G of this part) for additional...

  3. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... copy of the following documents be provided to me: . —A representative of the news media affiliated with the ____ newspaper (magazine, television station, etc.) and this request is made as part of...

  4. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., passport card, driver's license, ID card, etc.), or one form of unexpired identification with a photograph... license, ID card, etc.) and a properly authenticated birth certificate. If you make your request by...

  5. DIRECT-ACTING, DNA-DAMAGING AS (III)-METHYLATED SPECIES: IMPLICATIONS FOR A CARCINOGENIC MECHANISM OF ACTION OF ARSENICALS

    EPA Science Inventory

    Direct-acting, DNA-damaging As (III)-methylated species: implications for a carcinogenic . mechanism of action of arsenicals

    Inorganic arsenic (iAs, arsenite and arsenate) has been thought to act as a carcinogen without reacting directly with DNA; neither iAs nor the As(...

  6. 77 FR 61008 - Request for Comments Under the Paperwork Reduction Act, Section 3506

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-05

    ... Carr, Acting Director, Office of Clinical Research and Bioethics Policy, Office of Science Policy, NIH... 28, 2012. Sarah Carr, Acting Director, Office of Clinical Research and Bioethics Policy, Office...

  7. 78 FR 27260 - Comment Request: Antarctic Conservation Act Application Permit Form

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... type of object to be taken (plant, native mammal, or native bird. Use of the Information The purpose of... Act of 1978, Public Law 95-541, as amended by the Antarctic Science, Tourism, and Conservation Act...

  8. Clofarabine Acts as Radiosensitizer In Vitro and In Vivo by Interfering With DNA Damage Response

    SciTech Connect

    Cariveau, Mickael J.; Stackhouse, Murray; Cui Xiaoli; Tiwari, Kamal; Waud, William; Secrist, John A.; Xu Bo

    2008-01-01

    Purpose: Combination treatment with radiotherapy and chemotherapy has emerged as the dominant form of cancer adjuvant regimens in recent years. Clofarabine, a newly approved drug for pediatric leukemia, is a second-generation purine nucleoside analogue that can block DNA synthesis and inhibit DNA repair. Therefore, we hypothesized that clofarabine could work synergistically with radiotherapy to increase the tumor cell response. Methods and Materials: The effects of clofarabine on radiosensitivity have been established in several tumor cell lines in vitro and in vivo using colony-forming assays and tumor xenografts. The effect of clofarabine on the DNA damage response was also studied in vitro by measuring {gamma}-H2AX focus formation. Results: Clonogenic survival was significantly reduced in irradiated cells treated with clofarabine, demonstrating the strong radiosensitizing effect of clofarabine. Furthermore, clofarabine displayed a radiosensitizing effect that was greater than gemcitabine or 5-fluorouracil. We also found that low doses of clofarabine can prolong the presence of radiation-induced {gamma}-H2AX nuclear focus formation, and high doses of clofarabine can induce DNA double-strand breaks, suggesting that clofarabine can interfere with DNA damage response pathways. In addition, clofarabine-induced radiosensitization was also established in vivo using a colorectal cancer model, DLD-1, in athymic nude mice. When combined with fractionated radiotherapy, a moderate dose of clofarabine led to a significant increase in tumor growth inhibition. Conclusion: Clofarabine acts as a powerful radiosensitizer both in vitro and in vivo by interfering with the DNA damage response.

  9. TREX1 acts in degrading damaged DNA from drug-treated tumor cells

    PubMed Central

    Wang, Chuan-Jen; Lam, Wing; Bussom, Scott; Chang, Hua-Mei; Cheng, Yung-Chi

    2009-01-01

    The major mammalian exonuclease TREX1 has been proposed to play a role in DNA repair and drug resistance. However, no cellular evidence substantiates this claim. Recent reports indicate TREX1’s involvement in autoimmunity. To further understand its role, we studied TREX1 expression and functionality in anticancer drug-treated tumor cells. We report that the expression and localization of TREX1 are cell-type dependent. Camptothecin and other DNA damaging agents induced both TREX1 protein and its mRNA in a dose- and time-dependent manner. Using a TREX1-inducible cell line, we performed clonogenic assays and found no change in sensitivity of the cells to the agents upon TREX1 induction, suggesting that TREX1 may not play a role in DNA repair or drug sensitivity. Nevertheless, TREX1 serves as a key enzyme in the degradation of DNA from dying cells leading to less cellular DNA. Ubiquitously expressed in normal tissues, TREX1 may act in degrading DNA in all cell types undergoing a dying process before phagocytosis occurs. PMID:19617005

  10. 28 CFR 513.61 - Freedom of Information Act requests by inmates.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... or her Inmate Central File. (b) An inmate may make a request for access to documents in his or her Inmate Central File or Medical File (including documents which have been withheld from disclosure during... concerning the inmate which are not contained in the Inmate Central File or Medical File. Staff shall...

  11. Does Instruction Alter the Naturalistic Pattern of Pragmatic Development? A Case of Request Speech Act

    ERIC Educational Resources Information Center

    Taguchi, Naoko; Naganuma, Naeko; Budding, Carlos

    2015-01-01

    This study examined the effects of explicit instruction on the development of pragmatic competence in L2 English. The study is based on Taguchi's (2012) study conducted in an English-medium university in Japan, which revealed patterns of change in Japanese EFL students' production of requests in high- and low-imposition situations. Students showed…

  12. 77 FR 59224 - Comment Request for Information Collection for the Workforce Investment Act (WIA) Management...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    ... is the structure of the file. ETA is requesting permission to modify the WIASRD layout so as to make... individual record layout. The modified WIASRD layout will utilize the exact same variable definitions and data structures as the WISPR individual record layout rendering full scale WISPR implementation...

  13. 28 CFR 513.68 - Time limits for responses to Freedom of Information Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requests. Consistent with sound administrative practice and the provisions of 28 CFR 16.1, the Bureau... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Time limits for responses to Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom...

  14. 28 CFR 513.68 - Time limits for responses to Freedom of Information Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... requests. Consistent with sound administrative practice and the provisions of 28 CFR 16.1, the Bureau... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Time limits for responses to Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom...

  15. 28 CFR 513.68 - Time limits for responses to Freedom of Information Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... requests. Consistent with sound administrative practice and the provisions of 28 CFR 16.1, the Bureau... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Time limits for responses to Freedom of... JUSTICE GENERAL MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom...

  16. Cis-acting signals modulate the efficiency of programmed DNA elimination in Paramecium tetraurelia.

    PubMed

    Ferro, Diana; Lepennetier, Gildas; Catania, Francesco

    2015-09-30

    In Paramecium, the regeneration of a functional somatic genome at each sexual event relies on the elimination of thousands of germline DNA sequences, known as Internal Eliminated Sequences (IESs), from the zygotic nuclear DNA. Here, we provide evidence that IESs' length and sub-terminal bases jointly modulate IES excision by affecting DNA conformation in P. tetraurelia. Our study reveals an excess of complementary base pairing between IESs' sub-terminal and contiguous sites, suggesting that IESs may form DNA loops prior to cleavage. The degree of complementary base pairing between IESs' sub-terminal sites (termed Cin-score) is positively associated with IES length and is shaped by natural selection. Moreover, it escalates abruptly when IES length exceeds 45 nucleotides (nt), indicating that only sufficiently large IESs may form loops. Finally, we find that IESs smaller than 46 nt are favored targets of the cellular surveillance systems, presumably because of their relatively inefficient excision. Our findings extend the repertoire of cis-acting determinants for IES recognition/excision and provide unprecedented insights into the distinct selective pressures that operate on IESs and somatic DNA regions. This information potentially moves current models of IES evolution and of mechanisms of IES recognition/excision forward. PMID:26304543

  17. 77 FR 62494 - Proposed Information Collection; Comment Request; Evaluations of Coastal Zone Management Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-15

    ...; Evaluations of Coastal Zone Management Act Programs--State Coastal Management Programs and National Estuarine... Management Act of 1972, as amended (CZMA; 16 U.S.C. 1451 et seq.) requires that state coastal management... state coastal management programs be evaluated concerning the extent to which the state has...

  18. 75 FR 43485 - Proposed Information Collection; Comment Request; South Pacific Tuna Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-26

    ... Pacific Tuna Act AGENCY: National Oceanic and Atmospheric Administration (NOAA). ACTION: Notice. SUMMARY... information is required to meet U.S. obligations under the Treaty. The Treaty authorizes U.S. tuna vessels to fish within fishing zones of a large region of the Pacific Ocean. The South Pacific Tuna Act of...

  19. 78 FR 48860 - Proposed Information Collection; Comment Request; South Pacific Tuna Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-12

    ... Pacific Tuna Act AGENCY: National Oceanic and Atmospheric Administration (NOAA), Commerce. ACTION: Notice... under the Treaty. The Treaty authorizes U.S. tuna vessels to fish within fishing zones of a large region of the Pacific Ocean. The South Pacific Tuna Act of 1988 (16 U.S.C. 973-973r) and U.S....

  20. 28 CFR 513.67 - Fees for Freedom of Information Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Department of Justice regulations contained in 28 CFR 16.10. ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Fees for Freedom of Information Act... MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act...

  1. 28 CFR 513.67 - Fees for Freedom of Information Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Department of Justice regulations contained in 28 CFR 16.10. ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Fees for Freedom of Information Act... MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act...

  2. 28 CFR 513.67 - Fees for Freedom of Information Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Department of Justice regulations contained in 28 CFR 16.10. ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Fees for Freedom of Information Act... MANAGEMENT AND ADMINISTRATION ACCESS TO RECORDS Release of Information Freedom of Information Act...

  3. 77 FR 59899 - Proposed Information Collection; Comment Request; Coastal Zone Management Act Walter B. Jones and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-01

    ... Zone Management Act Walter B. Jones and NOAA Excellence Awards AGENCY: National Oceanic and Atmospheric... of the Coastal Zone Management Act (CZMA) authorized an awards program to ``implement a program to promote excellence in coastal zone management by identifying and acknowledging outstanding...

  4. 77 FR 37923 - Comment Request for Information Collection for the Workforce Investment Act (WIA) Adult and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-25

    ... Investment Act (WIA) Adult and Dislocated Worker Programs Gold Standard Evaluation (WIA Evaluation); New... than 6 million people annually at a cost of over $3 billion (U.S. Department of Labor, Fiscal Year 2012...--adults and dislocated workers in this group can receive any WIA service for which they are eligible;...

  5. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... describe the record that you want in enough detail to enable the Privacy Act Officer to locate the system... or identifying number of each system of records in which you believe it is kept, and the date, title... documentation that you think would be helpful, including an annotated copy of the record. (e) How do I...

  6. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Privacy Act Officer to locate the system of records containing it with a reasonable amount of effort... compiled, the name or identifying number of each system of records in which you believe it is kept, and the... question and the system of records in which the record is located, describe the amendment or...

  7. 76 FR 33413 - Proposed Renewal Without Change; Comment Request; Nine Bank Secrecy Act Recordkeeping Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... international terrorism was added by section 358 of the Uniting and Strengthening America by Providing Appropriate Tools Required to Intercept and Obstruct Terrorism (USA PATRIOT) Act of 2001, Pubic Law 107-56... intelligence or counter-intelligence activities, including analysis, to protect against international...

  8. 78 FR 27187 - Proposed Information Collection; Comment Request; Green Sturgeon Endangered Species Act Take...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... prohibitions, local, state, and federal agencies, non-governmental organizations, academic researchers, and... Species Act (ESA) were promulgated for the species on June 2, 2010 (75 FR 30714) (the final ESA 4(d) Rule... schedule to be determined by National Marine Fisheries Service (NMFS) staff. This information is used...

  9. 75 FR 5346 - Comment Request for Information Collection for Workforce Investment Act National Emergency Grant...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-02

    .... ADDRESSES: Submit written comments to Jeanette Provost, Office of National Response, Room C-5311, U.S... . SUPPLEMENTARY INFORMATION: I. Background: The information collection is necessary for the U.S. Department of... Investment Act's National Emergency Grant Program: Application and Reporting Procedures (OMB Control No....

  10. Ribonucleotides Misincorporated into DNA Act as Strand-Discrimination Signals in Eukaryotic Mismatch Repair

    PubMed Central

    Ghodgaonkar, Medini Manohar; Lazzaro, Federico; Olivera-Pimentel, Maite; Artola-Borán, Mariela; Cejka, Petr; Reijns, Martin A.; Jackson, Andrew P.; Plevani, Paolo; Muzi-Falconi, Marco; Jiricny, Josef

    2013-01-01

    Summary To improve replication fidelity, mismatch repair (MMR) must detect non-Watson-Crick base pairs and direct their repair to the nascent DNA strand. Eukaryotic MMR in vitro requires pre-existing strand discontinuities for initiation; consequently, it has been postulated that MMR in vivo initiates at Okazaki fragment termini in the lagging strand and at nicks generated in the leading strand by the mismatch-activated MLH1/PMS2 endonuclease. We now show that a single ribonucleotide in the vicinity of a mismatch can act as an initiation site for MMR in human cell extracts and that MMR activation in this system is dependent on RNase H2. As loss of RNase H2 in S.cerevisiae results in a mild MMR defect that is reflected in increased mutagenesis, MMR in vivo might also initiate at RNase H2-generated nicks. We therefore propose that ribonucleotides misincoporated during DNA replication serve as physiological markers of the nascent DNA strand. PMID:23603115

  11. 15 CFR 781.4 - U.S. Government requests for information needed to satisfy the requirements of the APR or the Act.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false U.S. Government requests for information needed to satisfy the requirements of the APR or the Act. 781.4 Section 781.4 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND...

  12. Assessment of ADHD Documentation from Candidates Requesting Americans with Disabilities Act (ADA) Accommodations for the National Board of Osteopathic Medical Examiners COMLEX Exam

    ERIC Educational Resources Information Center

    Joy, Javed A.; Julius, Rose J.; Akter, Rashida; Baron, David A.

    2010-01-01

    Purpose: Every year increasing numbers of candidates request special accommodations for high-stakes medical licensing examinations, due to ADHD, on the basis of the Americans with Disabilities Act (ADA). This poses significant challenges for both the applicant and the medical boards and has significant financial, legal, and ethical implications.…

  13. A novel cis-acting element required for DNA damage-inducible expression of yeast DIN7

    SciTech Connect

    Yoshitani, Ayako; Yoshida, Minoru; Ling Feng

    2008-01-04

    Din7 is a DNA damage-inducible mitochondrial nuclease that modulates the stability of mitochondrial DNA (mtDNA) in Saccharomyces cerevisiae. How DIN7 gene expression is regulated, however, has remained largely unclear. Using promoter sequence alignment, we found a highly conserved 19-bp sequence in the promoter regions of DIN7 and NTG1, which encodes an oxidative stress-inducible base-excision-repair enzyme. Deletion of the 19-bp sequence markedly reduced the hydroxyurea (HU)-enhanced DIN7 promoter activity. In addition, nuclear fractions prepared from HU-treated cells were used in in vitro band shift assays to reveal the presence of currently unidentified trans-acting factor(s) that preferentially bound to the 19-bp region. These results suggest that the 19-bp sequence is a novel cis-acting element that is required for the regulation of DIN7 expression in response to HU-induced DNA damage.

  14. DOE FY 2010 Budget Request and Recovery Act Funding for Energy Research, Development, Demonstration, and Deployment: Analysis and Recommendations

    SciTech Connect

    Anadon, Laura Diaz; Gallagher, Kelly Sims; Bunn, Matthew

    2009-06-01

    The combination of the FY 2010 budget request for the Department of Energy (DOE) and the portion of the American Recovery and Reinvestment Act of 2009 (ARRA) funds likely to be available in 2010 would (assuming that they would be split evenly between FY 2010 and FY 2011) result in a doubling in funding available for energy research, development, and deployment (ERD and D) from $3.6 billion in FY 2009 to $7.2 billion in FY 2010. Without the stimulus funds, DOE ERD and D investments in FY 2010 would decrease very slightly when compared to FY 2009. Excluding the $7.5 billion for the Advanced Technology Vehicles Manufacturing Loans in FY 2009, the FY 2010 budget request for deployment represents a 33 percent decrease from the FY 2009 levels from $520 million to $350 million. This decrease is largely due to the large amounts of funds appropriated in ARRA for DOE deployment programs, or $23.6 billion, which are three times greater than those appropriated in the FY 2009 budget. These very substantial funding amounts, coupled with the broad range of institutional innovations the administration is putting in place and movement toward putting a price on carbon emissions, will help accelerate innovation for a broad range of energy technologies. DOE's Advanced Research Projects Agency-Energy (ARPA-E) and the Energy Innovation Hubs are important initiatives that could contribute to two weak points of the government's energy innovation effort, namely funding high-risk projects in transformational technologies and in companies that have not traditionally worked with the government and strengthening the integration of basic and applied research in priority areas. Increasing the funding for different types of energy storage research, providing some support for exploring opportunities in coal-to-liquids with carbon capture and storage (CCS) and coal-and-biomass-to-liquids with CCS, and reducing funding for fission RD and D are other actions that Congress could take in the short

  15. 46 CFR 503.32 - Procedures for responding to requests made under the Freedom of Information Act.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... (iii) The need for consultation, which shall be conducted with all practicable speed, with another... the extent reasonably necessary to the proper processing of the particular request: (i) The need to... separate from the office processing the request; (ii) The need to search for, collect, and...

  16. Two high-mobility group box domains act together to underwind and kink DNA

    SciTech Connect

    Sánchez-Giraldo, R.; Acosta-Reyes, F. J.; Malarkey, C. S.; Saperas, N.; Churchill, M. E. A.; Campos, J. L.

    2015-06-30

    The crystal structure of HMGB1 box A bound to an unmodified AT-rich DNA fragment is reported at a resolution of 2 Å. A new mode of DNA recognition for HMG box proteins is found in which two box A domains bind in an unusual configuration generating a highly kinked DNA structure. High-mobility group protein 1 (HMGB1) is an essential and ubiquitous DNA architectural factor that influences a myriad of cellular processes. HMGB1 contains two DNA-binding domains, box A and box B, which have little sequence specificity but have remarkable abilities to underwind and bend DNA. Although HMGB1 box A is thought to be responsible for the majority of HMGB1–DNA interactions with pre-bent or kinked DNA, little is known about how it recognizes unmodified DNA. Here, the crystal structure of HMGB1 box A bound to an AT-rich DNA fragment is reported at a resolution of 2 Å. Two box A domains of HMGB1 collaborate in an unusual configuration in which the Phe37 residues of both domains stack together and intercalate the same CG base pair, generating highly kinked DNA. This represents a novel mode of DNA recognition for HMGB proteins and reveals a mechanism by which structure-specific HMG boxes kink linear DNA.

  17. DNA Ligase IV and Artemis Act Cooperatively to Suppress Homologous Recombination in Human Cells: Implications for DNA Double-Strand Break Repair

    PubMed Central

    Kurosawa, Aya; Saito, Shinta; So, Sairei; Hashimoto, Mitsumasa; Iwabuchi, Kuniyoshi; Watabe, Haruka; Adachi, Noritaka

    2013-01-01

    Nonhomologous end-joining (NHEJ) and homologous recombination (HR) are two major pathways for repairing DNA double-strand breaks (DSBs); however, their respective roles in human somatic cells remain to be elucidated. Here we show using a series of human gene-knockout cell lines that NHEJ repairs nearly all of the topoisomerase II- and low-dose radiation-induced DNA damage, while it negatively affects survival of cells harbouring replication-associated DSBs. Intriguingly, we find that loss of DNA ligase IV, a critical NHEJ ligase, and Artemis, an NHEJ factor with endonuclease activity, independently contribute to increased resistance to replication-associated DSBs. We also show that loss of Artemis alleviates hypersensitivity of DNA ligase IV-null cells to low-dose radiation- and topoisomerase II-induced DSBs. Finally, we demonstrate that Artemis-null human cells display increased gene-targeting efficiencies, particularly in the absence of DNA ligase IV. Collectively, these data suggest that DNA ligase IV and Artemis act cooperatively to promote NHEJ, thereby suppressing HR. Our results point to the possibility that HR can only operate on accidental DSBs when NHEJ is missing or abortive, and Artemis may be involved in pathway switching from incomplete NHEJ to HR. PMID:23967291

  18. Single-stranded DNA fragments of insect-specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control.

    PubMed

    Oberemok, Volodymyr V; Skorokhod, Oleksii A

    2014-07-01

    This paper focuses on the DNA insecticides as a novel preparation against gypsy moth (Lymantria dispar) based on DNA fragments of the anti-apoptotic gene of its nuclear polyhedrosis virus. It was found that the external application of a solution with two single-stranded DNA fragments from BIR and RING domains of LdMNPV (L.dispar multicapsid nuclear polyhedrosis virus) IAP-3 (inhibitor of apoptosis) gene induces a significantly higher mortality of gypsy moth caterpillars in comparison with the application of the control solutions. This effect does not depend on the infection of caterpillars with LdMNPV. The results also show that DNA insecticides based on LdMNPV IAP-3 gene fragments can be selective in action, and at least are not harmful to tobacco hornworm (Manduca sexta) and black cutworm (Agrotis ipsilon). Part of the gypsy moth genome cloned with the fragments of BIR and RING domains of LdMNPV IAP-3 gene as primers, has an overlap with the corresponding part of the LdMNPV IAP-3 gene and L.dispar IAP-1 mRNA for an inhibitor of apoptosis protein with the high cover by query, allows assuming that we cloned a part of gypsy moth anti-apoptosis gene. This finding gives the grounding that proposed here DNA insecticides might act through the blocking of the mechanisms involved in post transcriptional expression of insect anti-apoptosis genes. The results show the insecticidal potential of the viral genome fragments that can be used to create safe and relatively fast-acting DNA insecticides to control the quantity of gypsy moth populations, important task for forestry and agriculture. PMID:25052520

  19. 75 FR 19335 - Premium Review Process; Request for Comments Regarding Section 2794 of the Public Health Service Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... Comments Regarding Section 2794 of the Public Health Service Act AGENCY: Office of the Secretary, HHS... to the Public Health Service Act (the PHS Act). Section 2794 of the PHS Act requires the Secretary to... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH...

  20. Bovine papillomavirus type 1 DNA replication: the transcriptional activator E2 acts in vitro as a specificity factor.

    PubMed Central

    Bonne-Andréa, C; Tillier, F; McShan, G D; Wilson, V G; Clertant, P

    1997-01-01

    We previously devised cell-free conditions supporting efficient replication of bovine papillomavirus type 1 (BPV1) DNA (C. Bonne-Andréa, S. Santucci, and P. Clertant, J. Virol. 69:3201-3205, 1995): the use of highly active preparations of viral initiator protein E1, together with extract from a particular cell source, allowed the synthesis of complete DNA circles through successive rounds of replication; this occurred in the absence of the viral transcriptional activator E2, required in vivo for the replication of viral genomes. We now report that adding E2 to cell-free assays produced only slight effects both on the yield of E1-dependent DNA synthesis and on the quality of newly made DNA molecules when a template carrying a wild-type BPV1 replication origin (ori) was used. The performance of mouse cell extracts, unable to sustain efficient BPV1 DNA replication in the presence of E1 only, was likewise not improved by the addition of E2. In a proper in vitro environment, E1 is thus fully capable of efficiently initiating viral DNA synthesis by itself, an activity which is not enhanced by interaction with E2. An important effect, however, was detected: E2 totally suppressed the nonspecific replication of ori-defective DNA templates, otherwise observed in high E1 concentrations. We examined the requirements for building a minimal DNA sequence behaving in vitro as a specific ori sequence under stringent recognition conditions, i.e., in the presence of both E1 and E2. Only two elements, the 18-bp E1 binding palindrome and an AT-rich sequence, were required in cis to allow specific cell-free DNA replication; there seemed to be no need for an E2 binding site to ensure discrimination between specific ori templates and other DNA molecules, even in the presence of E2. This suggests that during the initiation of BPV1 DNA replication, at least in vitro, E2 acts as a specificity factor restricting the action of E1 to a defined ori sequence; this function, likely not demanding

  1. Regulation of DNA replication in irradiated cells by trans-acting factors

    SciTech Connect

    Wang, Y.; Huq, M.S.; Cheng, X.; Iliakis, G.

    1995-05-01

    We compared DNA replication activity in cytoplasmic extracts prepared from irradiated and nonirradiated HeLa cells using a simian virus 40 (SV40)-based in vitro replication assay. The assay measures semi-conservative DNA replication in a plasmid carrying the SV40 origin of replication and requires SV40 T antigen as the sole noncellular protein. The plasmid DNA used in the replication reaction is never exposed to radiation. We find that replication of plasmid DNA is significantly reduced when cytoplasmic extracts from irradiated cells are used. Since plasmid replication proceeds to completion in extracts from irradiated cells, the observed reduction in the overall replication activity is probably due to a reduction in the efficiency of initiation events. The degree of inhibition of DNA replication after exposure to 10, 30 and 50 Gy X rays as measured in vitro using this assay is similar to that measured in intact cells immediately before processing for extract preparation. These observations are compatible with the induction or activation by ionizing radiation of a factor(s) that inhibits in trans DNA replication. The results contribute to our understanding of the mechanism(s) developed by the cells to regulate DNA replication when exposed to clastogenic agents. Such processes may be of significance in the restoration of DNA integrity, and may define yet another checkpoint operating during S at the level of clusters of replicons. 26 refs., 4 figs.

  2. 75 FR 64776 - Initiation of Section 302 Investigation and Request for Public Comment: China-Acts, Policies and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-20

    ...The United States Trade Representative (``Trade Representative'') has initiated an investigation under section 302(a) of the Trade Act of 1974, as amended (``Trade Act''), with respect to acts, policies, and practices of the People's Republic of China (China) affecting trade and investment in green technology. The Trade Representative has initiated the investigation in response to a petition......

  3. 76 FR 2762 - Proposed Information Collection (VAAR Clause 852.236.89, Buy American Act) Activity: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-14

    ... AFFAIRS Proposed Information Collection (VAAR Clause 852.236.89, Buy American Act) Activity: Comment... Acquisition Regulation (VAAR) Clause 852.236-89, Buy American Act. OMB Control Number: 2900-0622. Type of Review: Extension of a currently approved collection. Abstract: The Buy American Act requires that...

  4. 76 FR 12360 - Request for Information on Implementation of the James Zadroga 9/11 Health and Compensation Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ... Register (76 FR 7862) on February 11, 2011 to accept oral comments from the public. Public Comment Period... the James Zadroga 9/ 11 Health and Compensation Act of 2010 (Pub. L. 111-347) AGENCY: National... Compensation Act of 2010 (Pub. L. 111- 347). A copy of the Act is posted on the Internet at...

  5. Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination

    PubMed Central

    Fredsøe, Jacob; Nielsen, Ida; Pedersen, Jakob Madsen; Bentsen, Iben Bach; Lisby, Michael; Bjergbaek, Lotte; Andersen, Anni H

    2015-01-01

    Faithful DNA replication with correct termination is essential for genome stability and transmission of genetic information. Here we have investigated the potential roles of Topoisomerase II (Top2) and the RecQ helicase Sgs1 during late stages of replication. We find that cells lacking Top2 and Sgs1 (or Top3) display two different characteristics during late S/G2 phase, checkpoint activation and accumulation of asymmetric X-structures, which are both independent of homologous recombination. Our data demonstrate that checkpoint activation is caused by a DNA structure formed at the strongest rDNA replication fork barrier (RFB) during replication termination, and consistently, checkpoint activation is dependent on the RFB binding protein, Fob1. In contrast, asymmetric X-structures are formed independent of Fob1 at less strong rDNA replication fork barriers. However, both checkpoint activation and formation of asymmetric X-structures are sensitive to conditions, which facilitate fork merging and progression of replication forks through replication fork barriers. Our data are consistent with a redundant role of Top2 and Sgs1 together with Top3 (Sgs1-Top3) in replication fork merging at rDNA barriers. At RFB either Top2 or Sgs1-Top3 is essential to prevent formation of a checkpoint activating DNA structure during termination, but at less strong rDNA barriers absence of the enzymes merely delays replication fork merging, causing an accumulation of asymmetric termination structures, which are solved over time. PMID:26630413

  6. 77 FR 58848 - Prescription Drug User Fee Act V Patient-Focused Drug Development; Consultation Meetings; Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ...The Food and Drug Administration (FDA) is issuing this notice to request that patient stakeholders notify FDA of their intention to participate in periodic consultation meetings on process issues related to FDA's patient-focused drug development initiative. This initiative is being conducted to fulfill FDA performance commitments made as part of the fifth authorization of the Prescription Drug......

  7. 76 FR 30835 - Requests for Modification or Revocation of Toxic Substances Control Act Section 5 Significant New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... detector, and sign the EPA visitor log. All visitor bags are processed through an X-ray machine and subject... January 6, 2010 (75 FR 773) (FRL-8794-5), EPA had intended to include these modification requests. In the... issue of December 22, 2008 (73 FR 78261) (FRL-8395-8), EPA included regulatory text to...

  8. 78 FR 32390 - Food and Drug Administration Safety and Innovation Act (FDASIA): Request for Comments on the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-30

    ...): Request for Comments on the Development of a Risk-Based Regulatory Framework and Strategy for Health Information Technology AGENCY: Office of the National Coordinator for Health Information Technology...: The Food and Drug Administration (FDA), Office of the National Coordinator for Health...

  9. Maternal, Infant and Early Childhood Home Visiting Program in the Affordable Care Act: Supplemental Instruction Request-February 2011

    ERIC Educational Resources Information Center

    Schmit, Stephanie

    2011-01-01

    The latest Evidence-Based Home Visiting Supplemental Information Request (SIR) has recently been released by the Department of Health and Human Services (HHS) with collaboration from the Health Resources and Services Administration (HRSA) and the Administration for Children and Families (ACF) as outlined in the first Funding Opportunity…

  10. 78 FR 61444 - Request for Public Comments on the Caribbean Basin Economic Recovery Act and the Caribbean Basin...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-03

    ... Representative (USTR). ACTION: Notice and request for public comment. SUMMARY: The Trade Policy Staff Committee....regulations.gov , please contact Don Eiss, Trade Policy Staff Committee, at (202) 395-3475 to make other... beneficiary countries; and its effect on advancing U.S. trade policy goals as set forth in the CBTPA....

  11. Walker-A Motif Acts to Coordinate ATP Hydrolysis with Motor Output in Viral DNA Packaging.

    PubMed

    delToro, Damian; Ortiz, David; Ordyan, Mariam; Sippy, Jean; Oh, Choon-Seok; Keller, Nicholas; Feiss, Michael; Catalano, Carlos E; Smith, Douglas E

    2016-07-01

    During the assembly of many viruses, a powerful ATP-driven motor translocates DNA into a preformed procapsid. A Walker-A "P-loop" motif is proposed to coordinate ATP binding and hydrolysis with DNA translocation. We use genetic, biochemical, and biophysical techniques to survey the roles of P-loop residues in bacteriophage lambda motor function. We identify 55 point mutations that reduce virus yield to below detectable levels in a highly sensitive genetic complementation assay and 33 that cause varying reductions in yield. Most changes in the predicted conserved residues K76, R79, G81, and S83 produce no detectable yield. Biochemical analyses show that R79A and S83A mutant proteins fold, assemble, and display genome maturation activity similar to wild-type (WT) but exhibit little ATPase or DNA packaging activity. Kinetic DNA cleavage and ATPase measurements implicate R79 in motor ring assembly on DNA, supporting recent structural models that locate the P-loop at the interface between motor subunits. Single-molecule measurements detect no translocation for K76A and K76R, while G81A and S83A exhibit strong impairments, consistent with their predicted roles in ATP binding. We identify eight residue changes spanning A78-K84 that yield impaired translocation phenotypes and show that Walker-A residues play important roles in determining motor velocity, pausing, and processivity. The efficiency of initiation of packaging correlates strongly with motor velocity. Frequent pausing and slipping caused by changes A78V and R79K suggest that these residues are important for ATP alignment and coupling of ATP binding to DNA gripping. Our findings support recent structural models implicating the P-loop arginine in ATP hydrolysis and mechanochemical coupling. PMID:27139643

  12. Flavonoids acting on DNA topoisomerases: recent advances and future perspectives in cancer therapy.

    PubMed

    Russo, P; Del Bufalo, A; Cesario, A

    2012-01-01

    Flavonoids, secondary metabolites ubiquitously produced in the plant kingdom, are low molecular weight polyphenolic molecules. They are characterized by variable chemical structures and show a vast array of biological activities (i.e... antiviral, antiinflammatory, antitumor, antimicrobial, estrogenic, antiestrogenic, antioxidant, mutagenic and antimutagenic) targeting different pathways. Some of these compounds such as Genistein, Daidzein or its synthetic derivative Phenoxodiol as well as Luteolin and Quercetin are able to inhibit DNA topoisomerases. This review discusses that Flavonoids targeting DNA topoisomerases may lead to novel drug development with anticancer potential. PMID:22998568

  13. 76 FR 37057 - Notice of Request for Extension of Approval of an Information Collection; Virus-Serum-Toxin Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    ... Collection; Virus-Serum-Toxin Act and Regulations AGENCY: Animal and Plant Health Inspection Service, USDA... Virus-Serum-Toxin Act and regulations. DATES: We will consider all comments that we receive on or before... (202) 690-2817 before coming. FOR FURTHER INFORMATION CONTACT: For information on the...

  14. 76 FR 52596 - Request for Comment Concerning Interpretations of the Magnuson-Moss Warranty Act; Rule Governing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... (40 FR 25721) providing interim guidance during the initial implementation of the Act. As the... the Federal Register (42 FR 36112) its Interpretations of the Magnuson-Moss Warranty Act to assist... Commission published in the Federal Register (40 FR 60188) its Rule Governing Disclosure of Written...

  15. The Intracellular DNA Sensor IFI16 Gene Acts as Restriction Factor for Human Cytomegalovirus Replication

    PubMed Central

    Gariano, Grazia Rosaria; Dell'Oste, Valentina; Bronzini, Matteo; Gatti, Deborah; Luganini, Anna; De Andrea, Marco; Gribaudo, Giorgio; Gariglio, Marisa; Landolfo, Santo

    2012-01-01

    Human interferon (IFN)-inducible IFI16 protein, an innate immune sensor of intracellular DNA, modulates various cell functions, however, its role in regulating virus growth remains unresolved. Here, we adopt two approaches to investigate whether IFI16 exerts pro- and/or anti-viral actions. First, the IFI16 gene was silenced using specific small interfering RNAs (siRNA) in human embryo lung fibroblasts (HELF) and replication of DNA and RNA viruses evaluated. IFI16-knockdown resulted in enhanced replication of Herpesviruses, in particular, Human Cytomegalovirus (HCMV). Consistent with this, HELF transduction with a dominant negative form of IFI16 lacking the PYRIN domain (PYD) enhanced the replication of HCMV. Second, HCMV replication was compared between HELFs overexpressing either the IFI16 gene or the LacZ gene. IFI16 overexpression decreased both virus yield and viral DNA copy number. Early and late, but not immediate-early, mRNAs and proteins were strongly down-regulated, thus IFI16 may exert its antiviral effect by impairing viral DNA synthesis. Constructs with the luciferase reporter gene driven by deleted or site-specific mutated forms of the HCMV DNA polymerase (UL54) promoter demonstrated that the inverted repeat element 1 (IR-1), located between −54 and −43 relative to the transcription start site, is the target of IFI16 suppression. Indeed, electrophoretic mobility shift assays and chromatin immunoprecipitation demonstrated that suppression of the UL54 promoter is mediated by IFI16-induced blocking of Sp1-like factors. Consistent with these results, deletion of the putative Sp1 responsive element from the HCMV UL44 promoter also relieved IFI16 suppression. Together, these data implicate IFI16 as a novel restriction factor against HCMV replication and provide new insight into the physiological functions of the IFN-inducible gene IFI16 as a viral restriction factor. PMID:22291595

  16. CAPACITY OF AQUATIC BACTERIA TO ACT AS RECIPIENTS FOR PLASMID DNA

    EPA Science Inventory

    Sixty-nine randomly selected, gram negative, freshwater bacterial isolates were screened for their ability to receive and express plasmids from Pseudomonas aeruginosa donors, using a plate mating technique. The plate mating technique identified 26 of the isolates as recipient-act...

  17. Scabin, a Novel DNA-acting ADP-ribosyltransferase from Streptomyces scabies.

    PubMed

    Lyons, Bronwyn; Ravulapalli, Ravikiran; Lanoue, Jason; Lugo, Miguel R; Dutta, Debajyoti; Carlin, Stephanie; Merrill, A Rod

    2016-05-20

    A bioinformatics strategy was used to identify Scabin, a novel DNA-targeting enzyme from the plant pathogen 87.22 strain of Streptomyces scabies Scabin shares nearly 40% sequence identity with the Pierisin family of mono-ADP-ribosyltransferase toxins. Scabin was purified to homogeneity as a 22-kDa single-domain enzyme and was shown to possess high NAD(+)-glycohydrolase (Km (NAD) = 68 ± 3 μm; kcat = 94 ± 2 min(-1)) activity with an RSQXE motif; it was also shown to target deoxyguanosine and showed sigmoidal enzyme kinetics (K0.5(deoxyguanosine) = 302 ± 12 μm; kcat = 14 min(-1)). Mass spectrometry analysis revealed that Scabin labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA. Several small molecule inhibitors were identified, and the most potent compounds were found to inhibit the enzyme activity with Ki values ranging from 3 to 24 μm PJ34, a well known inhibitor of poly-ADP-ribosyltransferases, was shown to be the most potent inhibitor of Scabin. Scabin was crystallized, representing the first structure of a DNA-targeting mono-ADP-ribosyltransferase enzyme; the structures of the apo-form (1.45 Å) and with two inhibitors (P6-E, 1.4 Å; PJ34, 1.6 Å) were solved. These x-ray structures are also the first high resolution structures of the Pierisin subgroup of the mono-ADP-ribosyltransferase toxin family. A model of Scabin with its DNA substrate is also proposed. PMID:27002155

  18. Different cis-acting DNA elements control expression of the human apolipoprotein AI gene in different cell types

    SciTech Connect

    Sastry, K.; Seedorf, U.; Karathanasis, S.K.

    1988-02-01

    In mammals, the gene coding for apolipoprotein AI (apoAI), a protein of the plasma lipid transport system, is expressed only in the liver and the intestine. A series of plasmids containing various lengths of sequences flanking the 5' end of the human apoAI gene were constructed and assayed for transient expression after introduction into cultured human hepatoma 9HepG2), colon carcinoma (Caco-2), and epithelial (HeLa) cells. The results showed that while most of these constructs are expressed in HepG2 and Caco-2 cells, none of them is expressed in HeLa cells. In addition, the results indicated that a DNA segment located between nucleotides -256 and -41 upstream from the transcription start site of this gene is necessary and sufficient for maximal levels of expression in HepG2 but not in Caco-2 cells, while a DNA segment located between nucleotides -2052 and -192 is required for maximal levels of expression in Caco-2 cells. Moreover, it was shown that the -256 to -41 DNA segment functions as a hepatoma cell-specific transcriptional enhancer with both homologous and heterologous promoters. These results indicate that different cis- and possibly trans-acting factors are involved in the establishment and subsequent regulation of expression of the apoAI gene in the mammalian liver and intestine.

  19. Workforce Investment Act: States' Spending Is on Track, But Better Guidance Would Improve Financial Reporting. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    General Accounting Office, Washington, DC.

    The U.S. Congress asked the Government Accounting Office (GAO) to determine the following: (1) to what extent states were spending their Workforce Investment Act (WIA) funds and whether the Department of Labor's (Labor's) data accurately reflected available funds; (2) what Labor did to assess how states were managing their WIA spending; and (3)…

  20. Workforce Investment Act: Interim Report on Status of Spending and States' Available Funds. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    Nilsen, Sigurd R.

    A study assessed whether the Department of Labor's (DOL's) information on states' Workforce Investment Act of 1998 (WIA) spending was a true reflection of states' available funds. The most recent available spending data from DOL and the 50 states were analyzed. Interviews were conducted with state workforce officials in nine states, local…

  1. A saturation screen for cis-acting regulatory DNA in the Hox genes of Ciona intestinalis

    SciTech Connect

    Keys, David N.; Lee, Byung-in; Di Gregorio, Anna; Harafuji, Naoe; Detter, Chris; Wang, Mei; Kahsai, Orsalem; Ahn, Sylvia; Arellano, Andre; Zhang, Quin; Trong, Stephan; Doyle, Sharon A.; Satoh, Noriyuki; Satou, Yutaka; Saiga, Hidetoshi; Christian, Allen; Rokhsar, Dan; Hawkins, Trevor L.; Levine, Mike; Richardson, Paul

    2005-01-05

    A screen for the systematic identification of cis-regulatory elements within large (>100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis. Randomly generated DNA fragments from bacterial artificial chromosomes containing two clusters of Hox genes were inserted into a vector upstream of a minimal promoter and lacZ reporter gene. A total of 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulatory activities were monitored in larvae. In sum, 21 separable cis-regulatory elements were found. These include eight Hox linked domains that drive expression in nested anterior-posterior domains of ectodermally derived tissues. In addition to vertebrate-like CNS regulation, the discovery of cis-regulatory domains that drive epidermal transcription suggests that C. intestinalis has arthropod-like Hox patterning in the epidermis.

  2. Cutting edge: a cis-acting DNA element targets AID-mediated sequence diversification to the chicken Ig light chain gene locus.

    PubMed

    Kothapalli, Nagarama; Norton, Darrell D; Fugmann, Sebastian D

    2008-02-15

    Somatic hypermutation and gene conversion are two closely related processes that increase the diversity of the primary Ig repertoire. Both processes are initiated by the activation-induced cytidine deaminase that converts cytosine residues to uracils in a transcription-dependent manner; these lesions are subsequently fixed in the genome by direct replication and error-prone DNA repair. Two alternative mechanisms were proposed to explain why this mutagenic activity is targeted almost exclusively to Ig loci: 1) specific cis-acting DNA sequences; or 2) very high levels of Ig gene transcription. In this study we now identify a novel 3' regulatory region in the chicken Ig light chain gene containing not only a classical transcriptional enhancer but also cis-acting DNA elements essential for targeting activation-induced cytidine deaminase-mediated sequence diversification to this locus. PMID:18250404

  3. DNA.

    ERIC Educational Resources Information Center

    Felsenfeld, Gary

    1985-01-01

    Structural form, bonding scheme, and chromatin structure of and gene-modification experiments with deoxyribonucleic acid (DNA) are described. Indicates that DNA's double helix is variable and also flexible as it interacts with regulatory and other molecules to transfer hereditary messages. (DH)

  4. 36 CFR 1007.4 - Preliminary processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requests. 1007.4 Section 1007.4 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE FREEDOM OF INFORMATION ACT § 1007.4 Preliminary processing of requests. (a) Scope of requests. Unless a... request under § 1007.6, the requester shall be notified of the delay as provided in § 1007.6(f)....

  5. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2013-04-01 2013-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  6. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2011-04-01 2011-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  7. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2010-04-01 2010-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  8. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2014-04-01 2014-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  9. 25 CFR 517.4 - Requirements for making requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requester is making a request for records about himself/herself, the requester should see 25 CFR 515.3 for... 25 Indians 2 2012-04-01 2012-04-01 false Requirements for making requests. 517.4 Section 517.4... INFORMATION ACT PROCEDURES § 517.4 Requirements for making requests. (a) How to make a FOIA request....

  10. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  11. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  12. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  13. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  14. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  15. A Citizen's Guide on Using the Freedom of Information Act and the Privacy Act of 1974 To Request Government Records. First Report by the Committee on Government Reform and Oversight. House of Representatives, 104th Congress, 1st Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Government Reform and Oversight.

    This report explains how to use the Freedom of Information Act (FOIA) and the Privacy Act of 1974. It reflects all changes to the laws made since 1977: major amendments to the Freedom of Information Act passed in 1974 and 1986; a major addition to the Privacy Act of 1974 was enacted in 1988; and minor amendments to the Privacy Act were made in…

  16. 7 CFR 1.6 - Aggregating requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Aggregating requests. 1.6 Section 1.6 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Official Records § 1.6 Aggregating requests. When an agency reasonably believes that a requester, or a group of requesters acting in...

  17. 7 CFR 1.6 - Aggregating requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Aggregating requests. 1.6 Section 1.6 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Official Records § 1.6 Aggregating requests. When an agency reasonably believes that a requester, or a group of requesters acting in...

  18. DNA

    ERIC Educational Resources Information Center

    Stent, Gunther S.

    1970-01-01

    This history for molecular genetics and its explanation of DNA begins with an analysis of the Golden Jubilee essay papers, 1955. The paper ends stating that the higher nervous system is the one major frontier of biological inquiry which still offers some romance of research. (Author/VW)

  19. 36 CFR 1008.15 - Requests for access to records: Initial decision.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... request has indicated a willingness to pay, the Privacy Act Officer shall notify the requester and shall not complete processing of the request until the requester has agreed, in writing, to pay fees as...

  20. 43 CFR 2.27 - How will a bureau handle a request for information that is contained in a Privacy Act system of...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... information that is contained in a Privacy Act system of records? (See DOI's Privacy Act regulations (Subpart... system of records? (See DOI's Privacy Act regulations (Subpart G of this part) for additional information... information, usually an original signature (see the appropriate Privacy Act system notice and, subpart G...

  1. 43 CFR 2.27 - How will a bureau handle a request for information that is contained in a Privacy Act system of...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... information that is contained in a Privacy Act system of records? (See DOI's Privacy Act regulations (Subpart... system of records? (See DOI's Privacy Act regulations (Subpart G of this part) for additional information... information, usually an original signature (see the appropriate Privacy Act system notice and, subpart G...

  2. 5 CFR 1830.4 - Requirements for requesting amendment of records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... quickest handling, both the request letter and envelope or any fax cover sheet should be clearly marked “Privacy Act Amendment Request.” Whether sent by mail or by fax, a Privacy Act amendment request will...

  3. 5 CFR 1830.4 - Requirements for requesting amendment of records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... quickest handling, both the request letter and envelope or any fax cover sheet should be clearly marked “Privacy Act Amendment Request.” Whether sent by mail or by fax, a Privacy Act amendment request will...

  4. 5 CFR 1830.4 - Requirements for requesting amendment of records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... quickest handling, both the request letter and envelope or any fax cover sheet should be clearly marked “Privacy Act Amendment Request.” Whether sent by mail or by fax, a Privacy Act amendment request will...

  5. The Crucial Role of Divalent Metal Ions in the DNA-Acting Efficacy and Inhibition of the Transcription of Dimeric Chromomycin A3

    PubMed Central

    Hsu, Chun-Wei; Chuang, Show-Mei; Wu, Wen-Ling; Hou, Ming-Hon

    2012-01-01

    Chromomycin A3 (Chro) is capable of forming a stable dimeric complex via chelation with Ni(II), Fe(II) and Co(II). According to the circular dichroism study, the dimer conformations are significantly different among the Fe(II)-, Co(II)-, and Ni(II)-containing dimeric Chro complexes; however, the dimer conformations were preserved at high temperatures. Furthermore, we conducted a systematic study to determine the effects of these divalent metal ions on the DNA-acting efficacy of dimeric Chro, including its DNA-binding affinity, DNA stabilization capacity, DNA cleavage activity, and the inhibition of transcription both in vitro and within cells. Kinetic analyses using surface plasmon resonance (SPR) showed that NiII(Chro)2 exhibited the highest Ka with a value of 1.26×107 M−1, which is approximately 1.6- and 3.7-fold higher than the Ka values obtained for CoII(Chro)2 and FeII(Chro)2, respectively. The Tm and ΔG values for the DNA duplex increased after the addition of drug complexes in the following order: NiII(Chro)2>CoII(Chro)2>FeII(Chro)2. In the DNA integrity assays, the DNA cleavage rate of CoII(Chro)2 (1.2×10−3 s−1) is higher than those of FeII(Chro)2 and NiII(Chro)2, which were calculated to be 1×10−4 and 3.1×10−4 s−1, respectively. Consistent with the SPR and UV melting results, NiII(Chro)2 possesses the highest inhibitory effect on in vitro transcription and c-myc transcription within cells compared to CoII(Chro)2 and FeII(Chro)2. By comparing the cytotoxicity among CoII(Chro)2, FeII(Chro)2, and NiII(Chro)2 to several cancer cell lines, our studies concluded that NiII(Chro)2 displayed more potential antitumor activities than CoII(Chro)2 and FeII(Chro)2 did due to its higher DNA-acting efficacy. Changes to the divalent metal ions in the dimeric Chro complexes have been correlated with improved anticancer profiles. The availability of new metal derivatives of Chro may introduce new possibilities for exploiting the unique properties of this

  6. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records identified by the requester exist, and Whether they...

  7. The SRS2 suppressor of rad6 mutations of Saccharomyces cerevisiae acts by channeling DNA lesions into the RAD52 DNA repair pathway

    SciTech Connect

    Schiestl, R.H.; Prakash, S.; Prakash, L. )

    1990-04-01

    rad6 mutants of Saccharomyces cerevisiae are defective in the repair of damaged DNA, DNA damage induced mutagenesis, and sporulation. In order to identify genes that can substitute for RAD6 function, the authors have isolated genomic suppressors of the UV sensitivity of rad6 deletion (rad6{Delta}) mutations and show that they also suppress the {gamma}-ray sensitivity but not the UV mutagenesis or sporulation defects of rad6. The suppressors show semidominance for suppression of UV sensitivity and dominance for suppression of {gamma}-ray sensitivity. The six suppressor mutations they isolated are all alleles of the same locus and are also allelic to a previously described suppressor of the rad6-1 nonsense mutation, SRS2. They show that suppression of rad6{Delta} is dependent on the RAD52 recombinational repair pathway since suppression is not observed in the rad6{Delta} SRS2 strain containing an additional mutation in either the RAD51, RAD52, RAD54, RAD55 or RAD57 genes. Possible mechanisms by which SRS2 may channel unrepaired DNA lesions into the RAD52 DNA repair pathway are discussed.

  8. 36 CFR 1007.3 - Requests for records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Requests for records. 1007.3 Section 1007.3 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE FREEDOM OF INFORMATION ACT § 1007.3 Requests for records. (a) Submission of requests. A request to inspect or copy records shall be submitted to the Presidio...

  9. cis-Acting sequences in addition to donor and acceptor sites are required for template switching during synthesis of plus-strand DNA for duck hepatitis B virus.

    PubMed Central

    Havert, M B; Loeb, D D

    1997-01-01

    A characteristic of all hepadnaviruses is the relaxed-circular conformation of the DNA genome within an infectious virion. Synthesis of the relaxed-circular genome by reverse transcription requires three template switches. These template switches, as for the template switches or strand transfers of other reverse-transcribing genetic elements, require repeated sequences (the donor and acceptor sites) between which a complementary strand of nucleic acid is transferred. The mechanism for each of the template switches in hepadnaviruses is poorly understood. To determine whether sequences other than the donor and acceptor sites are involved in the template switches of duck hepatitis B virus (DHBV), a series of molecular clones which express viral genomes bearing deletion mutations were analyzed. We found that three regions of the DHBV genome, which are distinct from the donor and acceptor sites, are required for the synthesis of relaxed-circular DNA. One region, located near the 3' end of the minus-strand template, is required for the template switch that circularizes the genome. The other two regions, located in the middle of the genome and near DR2, appear to be required for plus-strand primer translocation. We speculate that these cis-acting sequences may play a role in the organization of the minus-strand DNA template within the capsid particle so that it supports efficient template switching during plus-strand DNA synthesis. PMID:9188603

  10. The strain-specific cis-acting element of beet curly top geminivirus DNA replication maps to the directly repeated motif of the ori.

    PubMed

    Choi, I R; Stenger, D C

    1996-12-01

    Strains of beet curly top geminivirus (BCTV) possess distinct cis- and trans-acting replication specificity elements which are not separately interchangeable among strains. Analysis of the replication competency of chimeric BCTV genomes, in which portions of the origin of DNA replication (ori) were derived from heterologous BCTV strains, have permitted identification of an essential cis-acting element governing strain-specific replication in a subgroup II geminivirus. Our studies indicate that the cis-acting element responsible for strain-specific replication properties resides within the directly repeated motif of the BCTV ori. Transient replication assays conducted in leaf disks and complementation experiments conducted in whole plants indicated that the trans-acting replication specificity element, residing within the amino-terminal region of the C1 Rep protein, may recognize and replicate a chimeric BCTV genome containing a heterologous ori so long as all or portions of the core element of the directly repeated motif are derived from the same strain as the Rep protein. As Rep protein binding to the core element of the directly repeated motif has been demonstrated by others to be essential for replication of subgroup III geminiviruses, our results support the hypothesis that replication specificity of subgroup II viruses is governed by processes similar to that of subgroup III viruses. However, a second cis-acting element of the ori, which appears to contribute to subgroup III virus replication specificity, does not seem to be required for replication specificity among the subgroup II viruses examined. Nonetheless, a potential role for a second cis-acting element in the BCTV ori contributing to maximal replication cannot be excluded. PMID:8941329

  11. The Nuclear DNA Sensor IFI16 Acts as a Restriction Factor for Human Papillomavirus Replication through Epigenetic Modifications of the Viral Promoters

    PubMed Central

    Lo Cigno, Irene; De Andrea, Marco; Borgogna, Cinzia; Albertini, Silvia; Landini, Manuela M.; Peretti, Alberto; Johnson, Karen E.; Chandran, Bala; Landolfo, Santo

    2015-01-01

    ABSTRACT The human interferon-inducible IFI16 protein, an innate immune sensor of intracellular DNA, was recently demonstrated to act as a restriction factor for human cytomegalovirus (HCMV) and herpes simplex virus 1 (HSV-1) infection by inhibiting both viral-DNA replication and transcription. Through the use of two distinct cellular models, this study provides strong evidence in support of the notion that IFI16 can also restrict human papillomavirus 18 (HPV18) replication. In the first model, an immortalized keratinocyte cell line (NIKS) was used, in which the IFI16 protein was knocked down through the use of small interfering RNA (siRNA) technology and overexpressed following transduction with the adenovirus IFI16 (AdVIFI16) vector. The second model consisted of U2OS cells transfected by electroporation with HPV18 minicircles. In differentiated IFI16-silenced NIKS-HPV18 cells, viral-load values were significantly increased compared with differentiated control cells. Consistent with this, IFI16 overexpression severely impaired HPV18 replication in both NIKS and U2OS cells, thus confirming its antiviral restriction activity. In addition to the inhibition of viral replication, IFI16 was also able to reduce viral transcription, as demonstrated by viral-gene expression analysis in U2OS cells carrying episomal HPV18 minicircles and HeLa cells. We also provide evidence that IFI16 promotes the addition of heterochromatin marks and the reduction of euchromatin marks on viral chromatin at both early and late promoters, thus reducing both viral replication and transcription. Altogether, these results argue that IFI16 restricts chromatinized HPV DNA through epigenetic modifications and plays a broad surveillance role against viral DNA in the nucleus that is not restricted to herpesviruses. IMPORTANCE Intrinsic immunity is mediated by cellular restriction factors that are constitutively expressed and active even before a pathogen enters the cell. The host nuclear factor IFI

  12. Cholera toxin B subunit acts as a potent systemic adjuvant for HIV-1 DNA vaccination intramuscularly in mice

    PubMed Central

    Hou, Jue; Liu, Ying; Hsi, Jenny; Wang, Hongzhi; Tao, Ran; Shao, Yiming

    2014-01-01

    Cholera toxin B subunit (CTB) was investigated as a classical mucosal adjuvant that can increase vaccine immunogenicity. In this study, we found out the in vitro efficacy of cholera toxin B subunit (CTB) in activating mice bone marrow-derived dendritic cells (BMDCs) through Toll-like receptor signaling pathways. In vitro RNA and transcriptional level profiling arrays revealed that CTB guides high levels of Th1 and Th2 type cytokines, inflammatory cytokines, and chemokines. Based on the robustness of these profiling results, we examined the induction of HIV Env-specific immunity by CTB co-inoculated with HIV Env DNA vaccine intramuscularly in vivo. CTB enhanced HIV-Env specific cellular immune responses in Env-specific IFN-γ ELISPOT, compared with DNA vaccine alone. Moreover, CTB induced high levels of Env specific humoral response and promoted antibody maturation after the third round of vaccination. This combination immunization strategy induced a Th2-type bias response which is indicative of a high ratio of IgG1/IgG2a. This study reports that CTB as a classical mucosal adjuvant could enhance HIV-1 DNA-based vaccine immunogenicity intramuscularly; therefore, these findings suggest that CTB could serve as an effective candidate adjuvant for DNA vaccination. PMID:24633335

  13. 20 CFR 401.35 - Your right to request records.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Act (see 20 CFR part 402) allows you to request information from SSA whether or not it is in a system... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Your right to request records. 401.35 Section... AND INFORMATION The Privacy Act § 401.35 Your right to request records. The Privacy Act gives you...

  14. 76 FR 65519 - Notice of Proposed Information Collection: Comment Request; Construction Complaint-Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-21

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection: Comment Request; Construction Complaint... Office of Management and Budget (OMB) for review, as required by the Paperwork Reduction Act. The... also lists the following information: Title of Proposal: Construction Complaint--Request for...

  15. 76 FR 70111 - Proposed Information Collection; Comment Request; Request for Investigation Under Section 232 of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Bureau of Industry and Security Proposed Information Collection; Comment Request; Request for Investigation Under Section 232 of the Trade Expansion Act AGENCY: Bureau of Industry and Security,...

  16. The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers

    PubMed Central

    Zech, Juergen; Godfrey, Emma Louise; Masai, Hisao; Hartsuiker, Edgar; Dalgaard, Jacob Zeuthen

    2015-01-01

    During S-phase replication forks can stall at specific genetic loci. At some loci, the stalling events depend on the replisome components Schizosaccharomyces pombe Swi1 (Saccharomyces cerevisiae Tof1) and Swi3 (S. cerevisiae Csm3) as well as factors that bind DNA in a site-specific manner. Using a new genetic screen we identified Mrc1 (S. cerevisiae Mrc1/metazoan Claspin) as a replisome component involved in replication stalling. Mrc1 is known to form a sub-complex with Swi1 and Swi3 within the replisome and is required for the intra-S phase checkpoint activation. This discovery is surprising as several studies show that S. cerevisiae Mrc1 is not required for replication barrier activity. In contrast, we show that deletion of S. pombe mrc1 leads to an approximately three-fold reduction in barrier activity at several barriers and that Mrc1’s role in replication fork stalling is independent of its role in checkpoint activation. Instead, S. pombe Mrc1 mediated fork stalling requires the presence of a functional copy of its phylogenetically conserved DNA binding domain. Interestingly, this domain is on the sequence level absent from S. cerevisiae Mrc1. Our study indicates that direct interactions between the eukaryotic replisome and the DNA are important for site-specific replication stalling. PMID:26201080

  17. ROSINA (RSI), a novel protein with DNA-binding capacity, acts during floral organ development in Antirrhinum majus.

    PubMed

    Roccaro, Mario; Li, Yubin; Masiero, Simona; Saedler, Heinz; Sommer, Hans

    2005-07-01

    Petal and stamen identity of the Antirrhinum majus flower is under the genetic control of the floral homeotic gene DEFICIENS (DEF). To isolate factors involved in the regulation of DEF gene activity, a promoter segment of this B-function gene, containing cis-acting regulatory elements, was used to identify the novel trans-acting factor ROSINA (RSI). RSI does not show an extended similarity with any gene product present in the database. Rather RSI constitutes a protein that contains domains similar to known proteins from organisms of different phyla. The capacity of RSI to bind a sequence element of the DEF promoter, its spatial and temporal expression pattern together with the phenotype of RSI-RNAi interference plants as well as RSI over-expression in Arabidopsis thaliana suggest that RSI is a putative regulator of DEF gene activity in A. majus. PMID:15998310

  18. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction of records. (a) Correction requests. Any person is entitled to request correction of his or her record(s) covered under the Act. The request...

  19. 10 CFR 9.41 - Requests for waiver or reduction of fees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....41 Energy NUCLEAR REGULATORY COMMISSION PUBLIC RECORDS Freedom of Information Act Regulations § 9.41... Freedom of Information Act requests, the request for a waiver or reduction of fees should be included in the initial Freedom of Information Act request letter. (2) Each request for a waiver or reduction...

  20. Medicaid program; increased Federal Medical Assistance Percentage changes under the Affordable Care Act of 2010. Final rule with request for comments.

    PubMed

    2013-04-01

    This final rule implements the provisions of the Patient Protection and Affordable Care Act of 2010 and the Health Care and Education Reconciliation Act of 2010 (collectively referred to as the Affordable Care Act) relating to the availability of increased Federal Medical Assistance Percentage (FMAP) rates for certain adult populations under states' Medicaid programs. This final rule implements and interprets the increased FMAP rates that will be applicable beginning January 1, 2014 and sets forth conditions for states to claim these increased FMAP rates. PMID:23556184

  1. 40 CFR 142.41 - Variance request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Variance request. 142.41 Section 142...) NATIONAL PRIMARY DRINKING WATER REGULATIONS IMPLEMENTATION Variances Issued by the Administrator Under Section 1415(a) of the Act § 142.41 Variance request. A supplier of water may request the granting of...

  2. 28 CFR 80.16 - Additional requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Additional requests. 80.16 Section 80.16 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) FOREIGN CORRUPT PRACTICES ACT OPINION PROCEDURE § 80.16 Additional requests. Additional requests for FCPA Opinions may be filed with the...

  3. 28 CFR 80.16 - Additional requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Additional requests. 80.16 Section 80.16 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) FOREIGN CORRUPT PRACTICES ACT OPINION PROCEDURE § 80.16 Additional requests. Additional requests for FCPA Opinions may be filed with the...

  4. 28 CFR 80.16 - Additional requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Additional requests. 80.16 Section 80.16 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) FOREIGN CORRUPT PRACTICES ACT OPINION PROCEDURE § 80.16 Additional requests. Additional requests for FCPA Opinions may be filed with the...

  5. 28 CFR 80.16 - Additional requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Additional requests. 80.16 Section 80.16 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) FOREIGN CORRUPT PRACTICES ACT OPINION PROCEDURE § 80.16 Additional requests. Additional requests for FCPA Opinions may be filed with the...

  6. 28 CFR 80.16 - Additional requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Additional requests. 80.16 Section 80.16 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) FOREIGN CORRUPT PRACTICES ACT OPINION PROCEDURE § 80.16 Additional requests. Additional requests for FCPA Opinions may be filed with the...

  7. Polymorphic tandem repeats within gene promoters act as modifiers of gene expression and DNA methylation in humans.

    PubMed

    Quilez, Javier; Guilmatre, Audrey; Garg, Paras; Highnam, Gareth; Gymrek, Melissa; Erlich, Yaniv; Joshi, Ricky S; Mittelman, David; Sharp, Andrew J

    2016-05-01

    Despite representing an important source of genetic variation, tandem repeats (TRs) remain poorly studied due to technical difficulties. We hypothesized that TRs can operate as expression (eQTLs) and methylation (mQTLs) quantitative trait loci. To test this we analyzed the effect of variation at 4849 promoter-associated TRs, genotyped in 120 individuals, on neighboring gene expression and DNA methylation. Polymorphic promoter TRs were associated with increased variance in local gene expression and DNA methylation, suggesting functional consequences related to TR variation. We identified >100 TRs associated with expression/methylation levels of adjacent genes. These potential eQTL/mQTL TRs were enriched for overlaps with transcription factor binding and DNaseI hypersensitivity sites, providing a rationale for their effects. Moreover, we showed that most TR variants are poorly tagged by nearby single nucleotide polymorphisms (SNPs) markers, indicating that many functional TR variants are not effectively assayed by SNP-based approaches. Our study assigns biological significance to TR variations in the human genome, and suggests that a significant fraction of TR variations exert functional effects via alterations of local gene expression or epigenetics. We conclude that targeted studies that focus on genotyping TR variants are required to fully ascertain functional variation in the genome. PMID:27060133

  8. Genome-wide characterization of cis-acting DNA targets reveals the transcriptional regulatory framework of opaque2 in maize.

    PubMed

    Li, Chaobin; Qiao, Zhenyi; Qi, Weiwei; Wang, Qian; Yuan, Yue; Yang, Xi; Tang, Yuanping; Mei, Bing; Lv, Yuanda; Zhao, Han; Xiao, Han; Song, Rentao

    2015-03-01

    Opaque2 (O2) is a transcription factor that plays important roles during maize endosperm development. Mutation of the O2 gene improves the nutritional value of maize seeds but also confers pleiotropic effects that result in reduced agronomic quality. To reveal the transcriptional regulatory framework of O2, we studied the transcriptome of o2 mutants using RNA sequencing (RNA-Seq) and determined O2 DNA binding targets using chromatin immunoprecipitation coupled to high-throughput sequencing (ChIP-Seq). The RNA-Seq analysis revealed 1605 differentially expressed genes (DEGs) and 383 differentially expressed long, noncoding RNAs. The DEGs cover a wide range of functions related to nutrient reservoir activity, nitrogen metabolism, stress resistance, etc. ChIP-Seq analysis detected 1686 O2 DNA binding sites distributed over 1143 genes. Overlay of the RNA-Seq and ChIP-Seq results revealed 35 O2-modulated target genes. We identified four O2 binding motifs; among them, TGACGTGG appears to be the most conserved and strongest. We confirmed that, except for the 16- and 18-kD zeins, O2 directly regulates expression of all other zeins. O2 directly regulates two transcription factors, genes linked to carbon and amino acid metabolism and abiotic stress resistance. We built a hierarchical regulatory model for O2 that provides an understanding of its pleiotropic biological effects. PMID:25691733

  9. Polymorphic tandem repeats within gene promoters act as modifiers of gene expression and DNA methylation in humans

    PubMed Central

    Quilez, Javier; Guilmatre, Audrey; Garg, Paras; Highnam, Gareth; Gymrek, Melissa; Erlich, Yaniv; Joshi, Ricky S.; Mittelman, David; Sharp, Andrew J.

    2016-01-01

    Despite representing an important source of genetic variation, tandem repeats (TRs) remain poorly studied due to technical difficulties. We hypothesized that TRs can operate as expression (eQTLs) and methylation (mQTLs) quantitative trait loci. To test this we analyzed the effect of variation at 4849 promoter-associated TRs, genotyped in 120 individuals, on neighboring gene expression and DNA methylation. Polymorphic promoter TRs were associated with increased variance in local gene expression and DNA methylation, suggesting functional consequences related to TR variation. We identified >100 TRs associated with expression/methylation levels of adjacent genes. These potential eQTL/mQTL TRs were enriched for overlaps with transcription factor binding and DNaseI hypersensitivity sites, providing a rationale for their effects. Moreover, we showed that most TR variants are poorly tagged by nearby single nucleotide polymorphisms (SNPs) markers, indicating that many functional TR variants are not effectively assayed by SNP-based approaches. Our study assigns biological significance to TR variations in the human genome, and suggests that a significant fraction of TR variations exert functional effects via alterations of local gene expression or epigenetics. We conclude that targeted studies that focus on genotyping TR variants are required to fully ascertain functional variation in the genome. PMID:27060133

  10. DNA Polymerase α (swi7) and the Flap Endonuclease Fen1 (rad2) Act Together in the S-Phase Alkylation Damage Response in S. pombe

    PubMed Central

    Koulintchenko, Milana; Vengrova, Sonya; Eydmann, Trevor; Arumugam, Prakash; Dalgaard, Jacob Z.

    2012-01-01

    Polymerase α is an essential enzyme mainly mediating Okazaki fragment synthesis during lagging strand replication. A specific point mutation in Schizosaccharomyces pombe polymerase α named swi7-1, abolishes imprinting required for mating-type switching. Here we investigate whether this mutation confers any genome-wide defects. We show that the swi7-1 mutation renders cells hypersensitive to the DNA damaging agents methyl methansulfonate (MMS), hydroxyurea (HU) and UV and incapacitates activation of the intra-S checkpoint in response to DNA damage. In addition we show that, in the swi7-1 background, cells are characterized by an elevated level of repair foci and recombination, indicative of increased genetic instability. Furthermore, we detect novel Swi1-, -Swi3- and Pol α- dependent alkylation damage repair intermediates with mobility on 2D-gel that suggests presence of single-stranded regions. Genetic interaction studies showed that the flap endonuclease Fen1 works in the same pathway as Pol α in terms of alkylation damage response. Fen1 was also required for formation of alkylation- damage specific repair intermediates. We propose a model to explain how Pol α, Swi1, Swi3 and Fen1 might act together to detect and repair alkylation damage during S-phase. PMID:23071723

  11. 77 FR 38637 - Proposed Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-28

    ...; Comment Request Proposed Projects Title: Plan for Foster Care and Adoption Assistance--Title IV-E. OMB No... Act (the Act) for each public child welfare agency requesting Federal funding for foster care, adoption assistance and guardianship assistance under the Act. Section 479B of the Act provides for...

  12. 75 FR 77663 - Information Collection Extension Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-13

    ... of the Secretary Information Collection Extension Request ACTION: Notice. SUMMARY: The Department of... comment on information collection extension request in accordance with the Paperwork Reduction Act of 1995... Information Report--29 CFR part 31 (Title VI of the Civil Rights Act), Nondiscrimination--Disability--29...

  13. 16 CFR 4.11 - Disclosure requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Register citations affecting § 4.11, see the List of CFR Sections Affected, which appears in the Finding... RULES § 4.11 Disclosure requests. (a) Freedom of Information Act requests—(1) Initial requests—(i) Form and contents; time of receipt. (A) A request under the provisions of the Freedom of Information Act,...

  14. 36 CFR 1007.5 - Action on initial requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Action on initial requests... INFORMATION ACT § 1007.5 Action on initial requests. (a) Authority. (1) Requests shall be decided by the FOIA... requester) shall be forwarded a reasonable number of days prior to the date on which disclosure is to...

  15. 10 CFR 1008.7 - Processing of requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Processing of requests. 1008.7 Section 1008.7 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.7 Processing of requests. (a) Receipt of a request made in accordance with §...

  16. 10 CFR 1008.7 - Processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Processing of requests. 1008.7 Section 1008.7 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.7 Processing of requests. (a) Receipt of a request made in accordance with §...

  17. 10 CFR 1008.7 - Processing of requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Processing of requests. 1008.7 Section 1008.7 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.7 Processing of requests. (a) Receipt of a request made in accordance with §...

  18. 10 CFR 1008.7 - Processing of requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Processing of requests. 1008.7 Section 1008.7 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.7 Processing of requests. (a) Receipt of a request made in accordance with §...

  19. 32 CFR 320.4 - Procedures for requesting information.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... accordance with “DoD Freedom of Information Act Program Regulation” (32 CFR part 286). (g) Requests for... 32 National Defense 2 2010-07-01 2010-07-01 false Procedures for requesting information. 320.4... requesting information. (a) Upon request in person or by mail, any individual, as defined in § 320.2,...

  20. 45 CFR 613.2 - Requesting access to records.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Requesting access to records. 613.2 Section 613.2 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PRIVACY ACT REGULATIONS § 613.2 Requesting access to records. (a) Where to make a request. You may make a request for access to NSF records about...

  1. 45 CFR 613.2 - Requesting access to records.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Requesting access to records. 613.2 Section 613.2 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PRIVACY ACT REGULATIONS § 613.2 Requesting access to records. (a) Where to make a request. You may make a request for access to NSF records about...

  2. 45 CFR 613.2 - Requesting access to records.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Requesting access to records. 613.2 Section 613.2 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION PRIVACY ACT REGULATIONS § 613.2 Requesting access to records. (a) Where to make a request. You may make a request for access to NSF records about...

  3. 18 CFR 1301.6 - Responses to requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 2 2012-04-01 2012-04-01 false Responses to requests. 1301.6 Section 1301.6 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.6 Responses to requests. (a) Acknowledgements of requests. On receipt of a request, the FOIA Officer...

  4. 6 CFR 5.6 - Responses to requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Responses to requests. 5.6 Section 5.6 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act § 5.6 Responses to requests. (a) Acknowledgements of requests. On receipt of a request, a component ordinarily...

  5. 29 CFR 19.4 - Contents of request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Contents of request. 19.4 Section 19.4 Labor Office of the Secretary of Labor RIGHT TO FINANCIAL PRIVACY ACT § 19.4 Contents of request. The formal written request..., business address and business phone number. The request shall also contain the following: (a) The...

  6. 36 CFR 1120.25 - Form of requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Form of requests. 1120.25... BOARD PUBLIC AVAILABILITY OF INFORMATION Information Available Upon Request § 1120.25 Form of requests... otherwise need not be in any particular form. Each request under the Freedom of Information Act should...

  7. 10 CFR 1304.105 - Requests for access to records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Requests for access to records. 1304.105 Section 1304.105 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.105 Requests for access to records. (a) All requests for records should include the following information: (1) Full name, address, and telephone number of requester. (2)...

  8. Interim final rules under the Paul Wellstone and Pete Domenici Mental Health Parity and Addiction Equity Act of 2008. Interim final rules with request for comments.

    PubMed

    2010-02-01

    This document contains interim final rules implementing the Paul Wellstone and Pete Domenici Mental Health Parity and Addiction Equity Act of 2008, which requires parity between mental health or substance use disorder benefits and medical/surgical benefits with respect to financial requirements and treatment limitations under group health plans and health insurance coverage offered in connection with a group health plan. PMID:20352665

  9. DNA adenine methyltransferase (Dam) controls the expression of the cytotoxic enterotoxin (act) gene of Aeromonas hydrophila via tRNA modifying enzyme-glucose-inhibited division protein (GidA)

    PubMed Central

    Erova, Tatiana E.; Kosykh, Valeri G.; Sha, Jian; Chopra, Ashok K.

    2013-01-01

    Aeromonas hydrophila is both a human and animal pathogen, and the cytotoxic enterotoxin (Act) is a crucial virulence factor of this bacterium because of its associated hemolytic, cytotoxic, and enterotoxic activities. Previously, to define the role of some regulatory genes in modulating Act production, we showed that deletion of a glucose-inhibited division gene (gidA) encoding tRNA methylase reduced Act levels, while overproduction of DNA adenine methyltransferase (Dam) led to a concomitant increase in Act-associated biological activities of a diarrheal isolate SSU of A. hydrophila. Importantly, there are multiple GATC binding sites for Dam within an upstream sequence of the gidA gene and one such target site in the act gene upstream region. We showed the dam gene to be essential for the viability of A. hydrophila SSU, and, therefore, to better understand the interaction of the encoding genes, Dam and GidA, in act gene regulation, we constructed a gidA in-frame deletion mutant of Escherichia coli GM28 (dam+) and GM33 (Δdam) strains. We then tested the expressional activity of the act and gidA genes by using a promoterless pGlow-TOPO vector containing a reporter green fluorescent protein (GFP). Our data indicated that in GidA+ strains of E. coli, constitutive methylation of the GATC site(s) by Dam negatively regulated act and gidA gene expression as measured by GFP production. However, in the ΔgidA strains, irrespective of the presence or absence of constitutively active Dam, we did not observe any alteration in the expression of the act gene signifying the role of GidA in positively regulating Act production. To determine the exact mechanism of how Dam and GidA influence Act, a real-time quantitative PCR (RT-qPCR) assay was performed. The analysis indicated an increase in gidA and act gene expression in the A. hydrophila Dam-overproducing strain, and these data matched with Act production in the E. coli GM28 strain. Thus, the extent of DNA methylation caused by

  10. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  11. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  12. 10 CFR 9.53 - Requests; how and where presented.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Requests by Individuals for Information, Access Or Amendment of Records Maintained About Them § 9.53... Information Act and Privacy Act Officer, by an appropriate method listed in § 9.6. Requests relating to... the appropriate system manager. (b) All written requests must be made to the Freedom of...

  13. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  14. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  15. 28 CFR 802.14 - Requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... pursuant to the Privacy Act, 5 U.S.C. 552a. Such a request must be made in writing and addressed to the... the envelope “Privacy Act Request.” (2) Your request will be considered received as of the date it is received by the Office of the General Counsel. For quickest possible handling, you should mark both...

  16. 78 FR 66995 - Freedom of Information, Privacy Act, and Government in the Sunshine Act Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-08

    ...The Privacy and Civil Liberties Oversight Board is finalizing regulations to implement the Freedom of Information Act, the Privacy Act of 1974, and the Government in the Sunshine Act. This rule describes the procedures for members of the public to request access to records. In addition, this rule also includes procedures for the Board's responses to these requests, including the timeframe for......

  17. 78 FR 28532 - Freedom of Information, Privacy Act, and Government in the Sunshine Act Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-15

    ...The Privacy and Civil Liberties Oversight Board is proposing regulations to implement the Freedom of Information Act, the Privacy Act of 1974, and the Government in the Sunshine Act. This proposed rulemaking describes the procedures for members of the public to request access to records. In addition, this notice also proposes procedures for the Board's responses to these requests, including......

  18. Prelinguistic Vocalizations Distinguish Pointing Acts

    ERIC Educational Resources Information Center

    Grunloh, Thomas; Liszkowski, Ulf

    2015-01-01

    The current study investigated whether point-accompanying characteristics, like vocalizations and hand shape, differentiate infants' underlying motives of prelinguistic pointing. We elicited imperative (requestive) and declarative (expressive and informative) pointing acts in experimentally controlled situations, and analyzed accompanying…

  19. 75 FR 27997 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION Notice of Proposed Information Collection Requests AGENCY: Department of Education. ACTION: Notice of Proposed Information Collection Requests. SUMMARY: The Acting Director, Information Collection...

  20. 75 FR 7264 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-18

    ... Notice of Proposed Information Collection Requests AGENCY: Department of Education. SUMMARY: The Acting Director, Information Collection Clearance Division, Regulatory Information Management Services, Office of Management, invites comments on the proposed information collection requests as required by the...

  1. 75 FR 66165 - Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-27

    ... BUDGET Information Collection Activities: Proposed Collection; Comment Request AGENCY: Office of Management and Budget, Office of Federal Financial Management. ACTION: Notice; request for comments... accordance with the Paperwork Reduction Act [75 FR 35507]. OMB Watch, Project on Government...

  2. 75 FR 67976 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-04

    ... requirements of Section 512 of the Recovery Act, set forth by the Office of Management and Budget (OMB) under... HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  3. 75 FR 13289 - Agency Information Collection Request, 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... requirements of Section 512 of the Recovery Act, set forth by the Office of Management and Budget (OMB) under... HUMAN SERVICES Agency Information Collection Request, 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  4. TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is a potential prognostic biomarker in lung cancer

    PubMed Central

    Liu, Wen-bin; Han, Fei; Jiang, Xiao; Chen, Hong-qiang; Zhao, Huan; Liu, Yong; Li, Yong-hong; Huang, Chuanshu; Cao, Jia; Liu, Jin-yi

    2015-01-01

    Epigenetic silencing of tumour suppressors contributes to the development and progression of lung cancer. We recently found that TMEM196 was hypermethylated in lung cancer. This study aimed to clarify its epigenetic regulation, possible roles and clinical significance. TMEM196 methylation correlated with loss of protein expression in chemical-induced rat lung pathologic lesions and human lung cancer tissues and cell lines. 5-aza-2′-deoxycytidine restored TMEM196 expression. Moreover, TMEM196 hypermethylation was detected in 61.2% of primary lung tumours and found to be associated with poor differentiation and pathological stage of lung cancer. Functional studies showed that ectopic re-expression of TMEM196 in lung cancer cells inhibited cell proliferation, clonogenicity, cell motility and tumour formation. However, TMEM196 knockdown increased cell proliferation and inhibited apoptosis and cell-cycle arrest. These effects were associated with upregulation of p21 and Bax, and downregulation of cyclin D1, c-myc, CD44 and β-catenin. Kaplan–Meier survival curves showed that TMEM196 downregulation was significantly associated with shortened survival in lung cancer patients. Multivariate analysis showed that patients with TMEM196 expression had a better overall survival. Our results revealed for the first time that TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is an independent prognostic factor of lung cancer. PMID:26056045

  5. Rapid DNA Act of 2014

    THOMAS, 113th Congress

    Rep. Sensenbrenner, F. James, Jr. [R-WI-5

    2014-12-03

    12/18/2014 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, and Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  6. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the requester... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act,...

  7. 78 FR 40151 - Agency Forms Undergoing Paperwork Reduction Act Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-03

    ... Paperwork Reduction Act (44 U.S.C. Chapter 35). To request a copy of these requests, call (404) 639-7570 or... Invasive Methicillin- 10 609 20/60 resistant Staphylococcus aureus ABCs Case Report Form. ABCs...

  8. 75 FR 25860 - Agency Forms Undergoing Paperwork Reduction Act Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-10

    ... Paperwork Reduction Act (44 U.S.C. Chapter 35). To request a copy of these requests, call the CDC Reports...) will use a randomized controlled trial to evaluate the effectiveness of the intervention. Depending...

  9. 75 FR 81659 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Affordable...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ...; Affordable Care Act Enrollment Opportunity Notice--Prohibition on Lifetime Limits ACTION: Notice. SUMMARY... Administration (EBSA) sponsored information collection request (ICR) titled, ``Affordable Care Act Enrollment... INFORMATION: The Patient Protection and Affordable Care Act (the Affordable Care Act)......

  10. Detection of cis- and trans-acting Factors in DNA Structure-Induced Genetic Instability Using In silico and Cellular Approaches

    PubMed Central

    Wang, Guliang; Zhao, Junhua; Vasquez, Karen M.

    2016-01-01

    Sequences that can adopt alternative DNA structures (i.e., non-B DNA) are very abundant in mammalian genomes, and recent studies have revealed many important biological functions of non-B DNA structures in chromatin remodeling, DNA replication, transcription, and genetic instability. Here, we provide results from an in silico web-based search engine coupled with cell-based experiments to characterize the roles of non-B DNA conformations in genetic instability in eukaryotes. The purpose of this article is to illustrate strategies that can be used to identify and interrogate the biological roles of non-B DNA structures, particularly on genetic instability. We have included unpublished data using a short H-DNA-forming sequence from the human c-MYC promoter region as an example, and identified two different mechanisms of H-DNA-induced genetic instability in yeast and mammalian cells: a DNA replication-related model of mutagenesis; and a replication-independent cleavage model. Further, we identified candidate proteins involved in H-DNA-induced genetic instability by using a yeast genetic screen. A combination of in silico and cellular methods, as described here, should provide further insight into the contributions of non-B DNA structures in biological functions, genetic evolution, and disease development. PMID:27532010

  11. Detection of cis- and trans-acting Factors in DNA Structure-Induced Genetic Instability Using In silico and Cellular Approaches.

    PubMed

    Wang, Guliang; Zhao, Junhua; Vasquez, Karen M

    2016-01-01

    Sequences that can adopt alternative DNA structures (i.e., non-B DNA) are very abundant in mammalian genomes, and recent studies have revealed many important biological functions of non-B DNA structures in chromatin remodeling, DNA replication, transcription, and genetic instability. Here, we provide results from an in silico web-based search engine coupled with cell-based experiments to characterize the roles of non-B DNA conformations in genetic instability in eukaryotes. The purpose of this article is to illustrate strategies that can be used to identify and interrogate the biological roles of non-B DNA structures, particularly on genetic instability. We have included unpublished data using a short H-DNA-forming sequence from the human c-MYC promoter region as an example, and identified two different mechanisms of H-DNA-induced genetic instability in yeast and mammalian cells: a DNA replication-related model of mutagenesis; and a replication-independent cleavage model. Further, we identified candidate proteins involved in H-DNA-induced genetic instability by using a yeast genetic screen. A combination of in silico and cellular methods, as described here, should provide further insight into the contributions of non-B DNA structures in biological functions, genetic evolution, and disease development. PMID:27532010

  12. Analysis of S.1844, the Clear Skies Act of 2003; S. 843, the Clean Air Planning Act of 2003; and S. 366, the Clean Power Act of 2003

    EIA Publications

    2004-01-01

    Senator James M. Inhofe requested that the Energy Information Administration (EIA) undertake analysis of S.843, the Clean Air Planning Act of 2003, introduced by Senator Thomas Carper; S.366, the Clean Power Act of 2003, introduced by Senator James Jeffords; and S.1844, the Clear Skies Act of 2003, introduced by Senator James M. Inhofe. The EIA received this request on March 19, 2004. This Service Report responds to his request.

  13. 78 FR 50449 - Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... Reduction Act of 1995 (PRA95) . This program helps to ensure that requested data can be provided in the... clearly understood, and the impact of collection requirements on respondents can be properly assessed. The... ``Telephone Point of Purchase Survey.'' A copy of the proposed information collection request (ICR) can...

  14. 36 CFR 1192.37 - Stop request.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Stop request. 1192.37 Section 1192.37 Parks, Forests, and Public Property ARCHITECTURAL AND TRANSPORTATION BARRIERS COMPLIANCE BOARD AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY GUIDELINES FOR TRANSPORTATION VEHICLES Buses, Vans and Systems § 1192.37 Stop request....

  15. 16 CFR 4.11 - Disclosure requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Disclosure requests. 4.11 Section 4.11 Commercial Practices FEDERAL TRADE COMMISSION ORGANIZATION, PROCEDURES AND RULES OF PRACTICE MISCELLANEOUS RULES § 4.11 Disclosure requests. (a) Freedom of Information Act requests—(1) Initial requests—(i) Form and contents; time of receipt. (A) A...

  16. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written... business phone number. The request shall also contain the following: (1) The identity of the customer...

  17. 36 CFR 810.3 - Action on requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Action on requests. 810.3 Section 810.3 Parks, Forests, and Public Property ADVISORY COUNCIL ON HISTORIC PRESERVATION FREEDOM OF INFORMATION ACT REGULATIONS § 810.3 Action on requests. (a) Once a requested record has been identified,...

  18. 32 CFR 806.6 - Prompt action on requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Prompt action on requests. 806.6 Section 806.6... FREEDOM OF INFORMATION ACT PROGRAM § 806.6 Prompt action on requests. (a) Examples of letters to FOIA... quickly, with limited impact on the responding units. The request clearly identifies the records with...

  19. 45 CFR 502.11 - Actions on requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Actions on requests. 502.11 Section 502.11 Public... § 502.11 Actions on requests. (a) The Administrative Officer or any employee acting in that official's... making the request setting forth the reasons for the extension and the date on which a determination...

  20. 5 CFR 1820.5 - Responses to requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Responses to requests. 1820.5 Section 1820.5 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS; PRODUCTION OF RECORDS OR TESTIMONY § 1820.5 Responses to requests. (a) General. Ordinarily, OSC shall have...

  1. 5 CFR 1820.5 - Responses to requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Responses to requests. 1820.5 Section 1820.5 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS; PRODUCTION OF RECORDS OR TESTIMONY § 1820.5 Responses to requests. (a) General. Ordinarily, OSC shall have...

  2. 5 CFR 1820.5 - Responses to requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Responses to requests. 1820.5 Section 1820.5 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS; PRODUCTION OF RECORDS OR TESTIMONY § 1820.5 Responses to requests. (a) General. Ordinarily, OSC shall have...

  3. 32 CFR 1662.3 - Identification of information requested.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Identification of information requested. 1662.3 Section 1662.3 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM FREEDOM OF INFORMATION ACT (FOIA) PROCEDURES § 1662.3 Identification of information requested. Any person who requests information under...

  4. 32 CFR 1662.3 - Identification of information requested.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Identification of information requested. 1662.3 Section 1662.3 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM FREEDOM OF INFORMATION ACT (FOIA) PROCEDURES § 1662.3 Identification of information requested. Any person who requests information under...

  5. 32 CFR 1662.3 - Identification of information requested.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Identification of information requested. 1662.3 Section 1662.3 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM FREEDOM OF INFORMATION ACT (FOIA) PROCEDURES § 1662.3 Identification of information requested. Any person who requests information under...

  6. 12 CFR 1024.36 - Requests for information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 8 2014-01-01 2014-01-01 false Requests for information. 1024.36 Section 1024.36 Banks and Banking BUREAU OF CONSUMER FINANCIAL PROTECTION REAL ESTATE SETTLEMENT PROCEDURES ACT (REGULATION X) Mortgage Servicing § 1024.36 Requests for information. (a) Information request. A servicer shall comply with the requirements of...

  7. 5 CFR 1820.5 - Responses to requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Responses to requests. 1820.5 Section 1820.5 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS; PRODUCTION OF RECORDS OR TESTIMONY § 1820.5 Responses to requests. (a) General. Ordinarily, OSC shall have...

  8. 5 CFR 2100.7 - Request for correction or amendment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Request for correction or amendment. 2100.7 Section 2100.7 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.7 Request for correction or amendment. (a) Requests to correct or amend a file shall be addressed to the system...

  9. 12 CFR 202.5 - Rules concerning requests for information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 2 2013-01-01 2013-01-01 false Rules concerning requests for information. 202... SYSTEM EQUAL CREDIT OPPORTUNITY ACT (REGULATION B) § 202.5 Rules concerning requests for information. (a) General rules—(1) Requests for information. Except as provided in paragraphs (b) through (d) of...

  10. 12 CFR 1002.5 - Rules concerning requests for information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 8 2013-01-01 2013-01-01 false Rules concerning requests for information. 1002... ACT (REGULATION B) § 1002.5 Rules concerning requests for information. (a) General rules—(1) Requests for information. Except as provided in paragraphs (b) through (d) of this section, a creditor...

  11. 12 CFR 202.5 - Rules concerning requests for information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 2 2012-01-01 2012-01-01 false Rules concerning requests for information. 202... SYSTEM EQUAL CREDIT OPPORTUNITY ACT (REGULATION B) § 202.5 Rules concerning requests for information. (a) General rules—(1) Requests for information. Except as provided in paragraphs (b) through (d) of...

  12. 12 CFR 1002.5 - Rules concerning requests for information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 8 2014-01-01 2014-01-01 false Rules concerning requests for information. 1002... ACT (REGULATION B) § 1002.5 Rules concerning requests for information. (a) General rules—(1) Requests for information. Except as provided in paragraphs (b) through (d) of this section, a creditor...

  13. 12 CFR 1002.5 - Rules concerning requests for information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 8 2012-01-01 2012-01-01 false Rules concerning requests for information. 1002... ACT (REGULATION B) § 1002.5 Rules concerning requests for information. (a) General rules—(1) Requests for information. Except as provided in paragraphs (b) through (d) of this section, a creditor...

  14. 6 CFR 5.3 - Requirements for making requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Requirements for making requests. 5.3 Section 5... AND INFORMATION Freedom of Information Act § 5.3 Requirements for making requests. (a) How made and... the statute. If you are making a request for records about yourself, see § 5.21(d) for...

  15. 6 CFR 5.3 - Requirements for making requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Requirements for making requests. 5.3 Section 5... AND INFORMATION Freedom of Information Act § 5.3 Requirements for making requests. (a) How made and... the statute. If you are making a request for records about yourself, see § 5.21(d) for...

  16. 6 CFR 5.3 - Requirements for making requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Requirements for making requests. 5.3 Section 5... AND INFORMATION Freedom of Information Act § 5.3 Requirements for making requests. (a) How made and... the statute. If you are making a request for records about yourself, see § 5.21(d) for...

  17. 32 CFR 1900.21 - Processing of requests for records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requester pursuant to 32 CFR 1900.33. In such event, the Agency will inform the requester in writing and... Information Act Amendments of 1996. (b) Database of “officially released information.” As an alternative to extensive tasking and as an accommodation to many requesters, the Agency maintains a database of...

  18. 39 CFR 3004.51 - Fees-request category.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 39 Postal Service 1 2012-07-01 2012-07-01 false Fees-request category. 3004.51 Section 3004.51 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT § 3004.51 Fees—request category. (a) The level of fee charged depends on the request category....

  19. 32 CFR 1900.21 - Processing of requests for records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... requester pursuant to 32 CFR 1900.33. In such event, the Agency will inform the requester in writing and... Information Act Amendments of 1996. (b) Database of “officially released information.” As an alternative to extensive tasking and as an accommodation to many requesters, the Agency maintains a database of...

  20. 40 CFR 2.104 - Responses to requests and appeals.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... seek judicial review to obtain the records without first making an administrative appeal. (b) On... notify you of the decision. If your request for expedited treatment is granted, the request will be given..., any appeal of that decision will be acted on expeditiously. (f) Grants of requests. Once an...

  1. 49 CFR 701.8 - Responses to requests.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 7 2013-10-01 2013-10-01 false Responses to requests. 701.8 Section 701.8 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL RAILROAD PASSENGER CORPORATION (AMTRAK) AMTRAK FREEDOM OF INFORMATION ACT PROGRAM § 701.8 Responses to requests. (a) Granting of requests. When an initial determination is...

  2. 49 CFR 701.8 - Responses to requests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 7 2012-10-01 2012-10-01 false Responses to requests. 701.8 Section 701.8 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL RAILROAD PASSENGER CORPORATION (AMTRAK) AMTRAK FREEDOM OF INFORMATION ACT PROGRAM § 701.8 Responses to requests. (a) Granting of requests. When an initial determination is...

  3. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  4. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  5. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of identity,...

  6. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  7. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of identity,...

  8. 32 CFR 326.8 - Procedures for requesting access.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Procedures for requesting access. 326.8 Section 326.8 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) PRIVACY PROGRAM NATIONAL RECONNAISSANCE OFFICE PRIVACY ACT PROGRAM § 326.8 Procedures for requesting access. (a) Request in writing. An...

  9. 22 CFR 171.32 - Request for access to records.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Request for access to records. 171.32 Section 171.32 Foreign Relations DEPARTMENT OF STATE ACCESS TO INFORMATION AVAILABILITY OF INFORMATION AND RECORDS TO THE PUBLIC Privacy Act Provisions § 171.32 Request for access to records. (a) Description of records sought. All requests for access to...

  10. 6 CFR 5.5 - Timing of responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Timing of responses to requests. 5.5 Section 5.5... INFORMATION Freedom of Information Act § 5.5 Timing of responses to requests. (a) In general. Components... the number of pages involved. If a component does so, it shall advise requesters in its slower...

  11. 5 CFR 1820.5 - Responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Responses to requests. 1820.5 Section 1820.5 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS; PRODUCTION OF RECORDS OR TESTIMONY § 1820.5 Responses to requests. (a) General. Ordinarily, OSC shall have...

  12. 32 CFR 806.26 - Addressing FOIA requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Addressing FOIA requests. 806.26 Section 806.26 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.26 Addressing FOIA requests. (a) FOIA requests concerning Air National Guard Inspector General...

  13. 15 CFR 4.4 - Requirements for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Requirements for making requests. 4.4 Section 4.4 Commerce and Foreign Trade Office of the Secretary of Commerce DISCLOSURE OF GOVERNMENT INFORMATION Freedom of Information Act § 4.4 Requirements for making requests. (a) A request for records...

  14. 18 CFR 1301.5 - Timing of responses to requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Timing of responses to requests. 1301.5 Section 1301.5 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.5 Timing of responses to requests. (a) In general, TVA ordinarily shall respond to requests according...

  15. 12 CFR 202.5 - Rules concerning requests for information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Rules concerning requests for information. 202... SYSTEM EQUAL CREDIT OPPORTUNITY ACT (REGULATION B) § 202.5 Rules concerning requests for information. (a) General rules—(1) Requests for information. Except as provided in paragraphs (b) through (d) of...

  16. 39 CFR 3004.42 - Tracking of requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Tracking of requests. 3004.42 Section 3004.42 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT § 3004.42 Tracking of requests. (a) Upon receipt of a request, the Commission shall assign a...

  17. 39 CFR 3004.42 - Tracking of requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 39 Postal Service 1 2014-07-01 2014-07-01 false Tracking of requests. 3004.42 Section 3004.42 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT § 3004.42 Tracking of requests. (a) Upon receipt of a request, the Commission shall assign a...

  18. 39 CFR 3004.42 - Tracking of requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 39 Postal Service 1 2013-07-01 2013-07-01 false Tracking of requests. 3004.42 Section 3004.42 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT § 3004.42 Tracking of requests. (a) Upon receipt of a request, the Commission shall assign a...

  19. 39 CFR 3004.42 - Tracking of requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 39 Postal Service 1 2011-07-01 2011-07-01 false Tracking of requests. 3004.42 Section 3004.42 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT § 3004.42 Tracking of requests. (a) Upon receipt of a request, the Commission shall assign a...

  20. 39 CFR 3004.42 - Tracking of requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 39 Postal Service 1 2012-07-01 2012-07-01 false Tracking of requests. 3004.42 Section 3004.42 Postal Service POSTAL REGULATORY COMMISSION PERSONNEL PUBLIC RECORDS AND FREEDOM OF INFORMATION ACT § 3004.42 Tracking of requests. (a) Upon receipt of a request, the Commission shall assign a...

  1. Food security of older adults requesting Older Americans Act Nutrition Program in Georgia can be validly measured using a short form of the U.S. Household Food Security Survey Module.

    PubMed

    Lee, Jung Sun; Johnson, Mary Ann; Brown, Arvine; Nord, Mark

    2011-07-01

    Food security is a newly recommended outcome measure for the Older Americans Act Nutrition Program (OAANP); however, it is unknown how best to evaluate the need for this program and assess its impact on a large scale. Therefore, we measured food security in all new OAANP participants and waitlisted applicants in Georgia between July and early November, 2008 (n = 4731) with the self-administered mail survey method used in the ongoing Georgia Performance Outcomes Measures project. We used a modified 6-item U.S. Household Food Security Survey Module (HFSSM) with a 30-d reference period and 2 reminder postcards. Approximately 33% of those identified completed the survey (n = 1594, mean age 74.6 ± 9.5 y, 68.6% female, 30.6% black). Most of the respondents (91%) completed all 6 food security questions, whereas 26 did not respond to any question. Infit and outfit statistics for each of the 6 questions were within an acceptable range. Psychometric properties observed in our food security data were generally similar to those in the nationally representative survey conducted by the Census Bureau and suggest that our food security statistics may be meaningfully compared with national food security statistics published by the USDA. Our findings suggest that food security can be reasonably measured by a short form of HFSSM in older adults requesting OAANP. Such methodology also can be used to estimate the extent of food insecurity and help guide program and policy decisions to meet the nutrition assistance needs of vulnerable older adults. PMID:21562242

  2. 32 CFR 324.14 - Relationship between the Privacy Act and the Freedom of Information Act.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Freedom of Information Act. 324.14 Section 324.14 National Defense Department of Defense (Continued... to Records § 324.14 Relationship between the Privacy Act and the Freedom of Information Act. Access... to the DFAS Freedom of Information Act Regulation. Access requests that specifically state...

  3. 32 CFR 324.14 - Relationship between the Privacy Act and the Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Freedom of Information Act. 324.14 Section 324.14 National Defense Department of Defense (Continued... to Records § 324.14 Relationship between the Privacy Act and the Freedom of Information Act. Access... to the DFAS Freedom of Information Act Regulation. Access requests that specifically state...

  4. 32 CFR 324.14 - Relationship between the Privacy Act and the Freedom of Information Act.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Freedom of Information Act. 324.14 Section 324.14 National Defense Department of Defense (Continued... to Records § 324.14 Relationship between the Privacy Act and the Freedom of Information Act. Access... to the DFAS Freedom of Information Act Regulation. Access requests that specifically state...

  5. 36 CFR 1250.14 - If I do not use FOIA to request records, will NARA treat my request differently?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false If I do not use FOIA to... AVAILABILITY AND USE OF FEDERAL RECORDS General Information About Freedom of Information Act (FOIA) Requests § 1250.14 If I do not use FOIA to request records, will NARA treat my request differently? Whether...

  6. 36 CFR 1250.14 - If I do not use FOIA to request records, will NARA treat my request differently?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false If I do not use FOIA to... AVAILABILITY AND USE OF FEDERAL RECORDS General Information About Freedom of Information Act (FOIA) Requests § 1250.14 If I do not use FOIA to request records, will NARA treat my request differently? Whether...

  7. 36 CFR 1250.14 - If I do not use FOIA to request records, will NARA treat my request differently?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false If I do not use FOIA to... AVAILABILITY AND USE OF FEDERAL RECORDS General Information About Freedom of Information Act (FOIA) Requests § 1250.14 If I do not use FOIA to request records, will NARA treat my request differently? Whether...

  8. 20 CFR 401.35 - Your right to request records.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... this Privacy Act right include— (a) Special procedures for access to certain medical records (see 5 U.S... Act (see 20 CFR part 402) allows you to request information from SSA whether or not it is in a...

  9. 20 CFR 401.35 - Your right to request records.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... this Privacy Act right include— (a) Special procedures for access to certain medical records (see 5 U.S... Act (see 20 CFR part 402) allows you to request information from SSA whether or not it is in a...

  10. 20 CFR 401.35 - Your right to request records.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... this Privacy Act right include— (a) Special procedures for access to certain medical records (see 5 U.S... Act (see 20 CFR part 402) allows you to request information from SSA whether or not it is in a...

  11. 20 CFR 401.35 - Your right to request records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... this Privacy Act right include— (a) Special procedures for access to certain medical records (see 5 U.S... Act (see 20 CFR part 402) allows you to request information from SSA whether or not it is in a...

  12. 75 FR 3211 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-20

    ...The Acting Director, Information Collection Clearance Division, Regulatory Information Management Services, Office of Management, invites comments on the proposed information collection requests as required by the Paperwork Reduction Act of...

  13. 75 FR 4053 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-26

    ...The Acting Director, Information Collection Clearance Division, Regulatory Information Management Services, Office of Management, invites comments on the proposed information collection requests as required by the Paperwork Reduction Act of...

  14. 75 FR 9189 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-01

    ...The Acting Director, Information Collection Clearance Division, Regulatory Information Management Services, Office of Management, invites comments on the proposed information collection requests as required by the Paperwork Reduction Act of...

  15. Freedom of Information Act

    USGS Publications Warehouse

    Newman, D.J.

    2012-01-01

    The Freedom of Information Act( FOIA), 5 U.S.C.§ 552, as amended, generally provides that any person has a right to request access to Federal agency records. The USGS proactively promotes information disclosure as inherent to its mission of providing objective science to inform decisionmakers and the general public. USGS scientists disseminate up-to-date and historical scientific data that are critical to addressing national and global priorities.

  16. 75 FR 59274 - Medicare and Medicaid Programs; Announcement of Application from Hospital Requesting Waiver for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-27

    ... section 1138(a)(2) of the Social Security Act (the Act). This notice requests comments from OPOs and the...)(C) of the Social Security Act (the Act) and our regulations at Sec. 482.45. Section 1138(a)(1)(A... United Network for Organ Sharing, and our regional offices. If necessary, we may request...

  17. 75 FR 21337 - Medicare and Medicaid Programs; Announcement of an Application From a Hospital Requesting Waiver...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-23

    ... accordance with section 1138(a)(2) of the Social Security Act (the Act). This notice requests comments from...)(C) of the Social Security Act (the Act) and our regulations at 42 CFR 482.45. Section 1138(a)(1)(A... United Network for Organ Sharing, and our regional offices. If necessary, we may request...

  18. 77 FR 60703 - Medicare and Medicaid Programs; Announcement of Application From Hospital Requesting Waiver for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-04

    ... Social Security Act (the Act). In addition, this notice requests comments from OPOs and the general...)(1)(C) of the Social Security Act (the Act) and our regulations at 42 CFR 482.45. Section 1138(a)(1... United Network for Organ Sharing, and our regional offices. If necessary, we may request...

  19. 2 CFR 176.120 - Determinations on late requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Determinations on late requests. 176.120... American Recovery and Reinvestment Act of 2009 § 176.120 Determinations on late requests. (a) If a... determination regarding the inapplicability of section 1605 of the Recovery Act on information required by §...

  20. 5 CFR 1630.6 - Action on request.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Action on request. 1630.6 Section 1630.6 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD PRIVACY ACT REGULATIONS § 1630.6 Action on request. (a) For TSP records, the record keeper designee, and for non-TSP records, the Privacy Act...

  1. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  2. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  3. 14 CFR 1212.201 - Requesting a record.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... request shall be processed as a Freedom of Information Act (FOIA) request under 14 CFR part 1206. If the....201 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS... appropriate system manager, or, if unknown, to the NASA Headquarters or Field Installation Information...

  4. 14 CFR 1212.201 - Requesting a record.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... request shall be processed as a Freedom of Information Act (FOIA) request under 14 CFR part 1206. If the....201 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS... appropriate system manager, or, if unknown, to the NASA Headquarters or Field Installation Information...

  5. 14 CFR 1212.201 - Requesting a record.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... request shall be processed as a Freedom of Information Act (FOIA) request under 14 CFR part 1206. If the....201 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS... appropriate system manager, or, if unknown, to the NASA Headquarters or Field Installation Information...

  6. 14 CFR § 1212.201 - Requesting a record.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... be processed as a Freedom of Information Act (FOIA) request under 14 CFR part 1206. If the records... Freedom of Information Act (FOIA) Office at NASA Headquarters or Field Center. The request should be...§ 1212.201 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY...

  7. 45 CFR 612.3 - Requirements for making requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... how to make a Privacy Act request (see NSF Privacy Act regulations at 45 CFR 613.2). (2) If you are... 612.3 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION.... The National Science Foundation has one Agency component. You may make a FOIA request for records...

  8. 32 CFR 300.7 - FOIA request processing procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE LOGISTICS AGENCY FREEDOM OF INFORMATION ACT PROGRAM... belong to an agency outside of DoD, the requester shall be told these are not agency records and, if possible, provide the name of the agency that may hold the records. No referral of the request is...

  9. 75 FR 345 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-05

    ... Notice of Proposed Information Collection Requests AGENCY: Department of Education. SUMMARY: The Acting... information technology. Dated: December 30, 2009. James Hyler, Acting Director, Information Collection... Attachments'' to view. Written requests for information should be addressed to U.S. Department of...

  10. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  11. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  12. 22 CFR 171.35 - Denials of requests; appeals.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 171.35 Foreign Relations DEPARTMENT OF STATE ACCESS TO INFORMATION AVAILABILITY OF INFORMATION AND RECORDS TO THE PUBLIC Privacy Act Provisions § 171.35 Denials of requests; appeals. If the Department denies a request for access to Privacy Act records, for amendment of such records, or for an...

  13. 12 CFR 1102.106 - Review of requests for amendment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Privacy Act Officer will: (1) Advise the individual making the request in writing of the extent to which... or in part, the Privacy Act Officer will: (1) Promptly advise the individual making the request in... individual in perfecting an appeal of the initial decision....

  14. 22 CFR 503.2 - Making a request.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 2 2014-04-01 2014-04-01 false Making a request. 503.2 Section 503.2 Foreign Relations BROADCASTING BOARD OF GOVERNORS FREEDOM OF INFORMATION ACT REGULATION § 503.2 Making a request. (a... (202) 260-4394. Write the words “Freedom of Information Act Request” on the envelope and letter....

  15. In-Silico docking of HIV-1 integrase inhibitors reveals a novel drug type acting on an enzyme/DNA reaction intermediate

    PubMed Central

    Savarino, Andrea

    2007-01-01

    Background HIV-1 integrase (IN) is an emerging drug target, as IN strand transfer inhibitors (INSTIs) are proving potent antiretroviral agents in clinical trials. One credible theory sees INSTIs as docking at the cellular (acceptor) DNA-binding site after IN forms a transitional complex with viral (donor) DNA. However, mapping of the DNA and INSTI binding sites within the IN catalytic core domain (CCD) has been uncertain. Methods Structural superimpositions were conducted using the SWISS PDB and Cn3D free software. Docking simulations of INSTIs were run by a widely validated genetic algorithm (GOLD). Results Structural superimpositions suggested that a two-metal model for HIV-1 IN CCD in complex with small molecule, 1-(5-chloroindol-3-yl)-3-(tetrazoyl)-1,3-propandione-ene (5CITEP) could be used as a surrogate for an IN/viral DNA complex, because it allowed replication of contacts documented biochemically in viral DNA/IN complexes or displayed by a crystal structure of the IN-related enzyme Tn5 transposase in complex with transposable DNA. Docking simulations showed that the fitness of different compounds for the catalytic cavity of the IN/5CITEP complex significantly (P < 0.01) correlated with their 50% inhibitory concentrations (IC50s) in strand transfer assays in vitro. The amino acids involved in inhibitor binding matched those involved in drug resistance. Both metal binding and occupation of the putative viral DNA binding site by 5CITEP appeared to be important for optimal drug/ligand interactions. The docking site of INSTIs appeared to overlap with a putative acceptor DNA binding region adjacent to but distinct from the putative donor DNA binding site, and homologous to the nucleic acid binding site of RNAse H. Of note, some INSTIs such as 4,5-dihydroxypyrimidine carboxamides/N-Alkyl-5-hydroxypyrimidinone carboxamides, a highly promising drug class including raltegravir/MK-0518 (now in clinical trials), displayed interactions with IN reminiscent of those

  16. 36 CFR 1250.4 - Who can file a FOIA request?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Who can file a FOIA request... Freedom of Information Act (FOIA) Requests § 1250.4 Who can file a FOIA request? Any individual, partnership, corporation, association, or government regardless of nationality may file a FOIA request....

  17. 36 CFR 1250.4 - Who can file a FOIA request?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Who can file a FOIA request... Freedom of Information Act (FOIA) Requests § 1250.4 Who can file a FOIA request? Any individual, partnership, corporation, association, or government regardless of nationality may file a FOIA request....

  18. 12 CFR 1202.5 - How do I request information from FHFA under FOIA?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... FOIA? 1202.5 Section 1202.5 Banks and Banking FEDERAL HOUSING FINANCE AGENCY ORGANIZATION AND OPERATIONS FREEDOM OF INFORMATION ACT § 1202.5 How do I request information from FHFA under FOIA? (a) Where To Send Your Request. FOIA requests must be in writing. You may make a request for FHFA records...

  19. 36 CFR 1250.4 - Who can file a FOIA request?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Who can file a FOIA request... Freedom of Information Act (FOIA) Requests § 1250.4 Who can file a FOIA request? Any individual, partnership, corporation, association, or government regardless of nationality may file a FOIA request....

  20. 36 CFR 1250.4 - Who can file a FOIA request?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Who can file a FOIA request... Freedom of Information Act (FOIA) Requests § 1250.4 Who can file a FOIA request? Any individual, partnership, corporation, association, or government regardless of nationality may file a FOIA request....

  1. 77 FR 5481 - Notice of Request for Extension of Approval of an Information Collection; Special Need Requests...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ... Animal and Plant Health Inspection Service Notice of Request for Extension of Approval of an Information Collection; Special Need Requests Under the Plant Protection Act AGENCY: Animal and Plant Health Inspection... accordance with the Paperwork Reduction Act of 1995, this notice announces the Animal and Plant...

  2. 75 FR 39692 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-12

    ... HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of... within 60-days. Proposed Project: Prevention and Wellness-Leveraging National Organizations OMB No. 0990... efforts beyond Recovery Act CPPW funding and support the National Prevention Media Initiative through...

  3. 75 FR 45118 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-02

    ... HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... collection for the proper performance of the agency's functions; (2) the accuracy of the estimated burden;...

  4. 75 FR 45120 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-02

    ... HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... collection for the proper performance of the agency's functions; (2) the accuracy of the estimated burden;...

  5. 77 FR 43826 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-26

    ... HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... proposed information collection for the proper performance of the agency's functions; (2) the accuracy...

  6. 76 FR 71037 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ... HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of... Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is publishing... proposed information collection for the proper performance of the agency's functions; (2) the accuracy...

  7. 75 FR 6033 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-05

    ... HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... proposed information collection for the proper performance of the agency's functions; (2) the accuracy...

  8. 75 FR 45118 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  9. 77 FR 29348 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  10. 75 FR 63479 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is..., Director, Office of Resources Management; Office of the Chief Information Officer. BILLING CODE 4150-45-P...

  11. 77 FR 61599 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Trade...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-10

    ... comments were previously requested via the Federal Register (77 FR 34037) on June 8, 2012 during a 60-day... Secret Claims for Emergency Planning and Community Right-to-Know Act (Renewal) AGENCY: Environmental... information collection request (ICR), ``Trade Secret Claims for Emergency Planning and Community...

  12. 76 FR 20987 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  13. 77 FR 70748 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Acid...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-27

    ... collection request (ICR), Acid Rain Program under Title IV of the Clean Air Act Amendments (Renewal) (EPA ICR... requested via the Federal Register (77 FR 4066) on July 10, 2012 during a 60-day comment period. This notice...'s public docket, visit http://www.epa.gov/dockets . Abstract: The Acid Rain Program was...

  14. 77 FR 4561 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-30

    ... HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... requirements. The lack of general awareness and understanding about the nature and extent of racial and...

  15. 76 FR 35444 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  16. 76 FR 78012 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  17. 76 FR 59130 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  18. 78 FR 56225 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Soil...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-12

    ... activities as described in this ICR (77 FR 39699, July 5, 2012). EPA did not receive any public comments... AGENCY Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Soil...) for review and approval in accordance with the Paperwork Reduction Act: ``Soil Fumigant...

  19. 75 FR 45121 - Agency Information Collection Request; 30-Day Public Comment Request; 30-Day Notice

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request; 30-Day Notice... Paperwork Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human...

  20. 75 FR 48969 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30 Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  1. 76 FR 10364 - Agency Information Collection Request. 30-Day Public Comment Request, Grants.gov

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request, Grants.gov AGENCY... Paperwork Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human...

  2. 76 FR 10035 - Agency Information Collection Request. 30-Day Public Comment Request, Grants.gov

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request, Grants.gov AGENCY... Paperwork Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human...

  3. 76 FR 10034 - Agency Information Collection Request. 30-Day Public Comment Request, Grants.gov

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request, Grants.gov AGENCY... Paperwork Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human...

  4. 75 FR 45120 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  5. 75 FR 63478 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  6. 76 FR 76165 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  7. 75 FR 27346 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  8. 76 FR 1616 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  9. 76 FR 17128 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  10. 76 FR 59701 - Agency Information Collection Request. 30 Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-27

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30 Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  11. 77 FR 4854 - Agency Information Collection Activities: Proposed Request and Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-31

    ..., 2011 at 76 FR 71105. Since we are revising the Privacy Act Statement, this is actually a revision of an... From the Federal Register Online via the Government Publishing Office SOCIAL SECURITY ADMINISTRATION Agency Information Collection Activities: Proposed Request and Comment Request The Social...

  12. 76 FR 67454 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-01

    ... HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... numbered 4.C and titled ] ``Previous Grants.gov Tracking Number''; (2) Modification of an existing...

  13. 76 FR 45902 - Agency Information Collection Activities: Proposed Request and Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... Internet or Automated Telephone Response System to conduct business with SSA. Type of Request: Request for... information collection as an extension on May 26, 2011 at 76 FR 30749. Since we are revising the Privacy Act... quality, utility, and clarity; and ways to minimize burden on respondents, including the use of...

  14. 76 FR 55065 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  15. 75 FR 48968 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ... HUMAN SERVICES Agency Information Collection Request. 30 Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... is a biennial survey of the blood collection and utilization community to produce reliable...

  16. 75 FR 22806 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... is a biennial survey of the blood collection and utilization community to produce reliable...

  17. 77 FR 5253 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of the Secretary, HHS. In compliance with the requirement of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Office of the Secretary...

  18. 75 FR 13288 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... conduct a nationwide survey which will use computer-assisted telephone interviews (CATI) to interview...

  19. 76 FR 10366 - Agency Information Collection Request; 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... HUMAN SERVICES Agency Information Collection Request; 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... Evaluation (ASPE). Abstract: This research leverages best practices in behavior change, interaction...

  20. 76 FR 71036 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  1. 76 FR 51980 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  2. 76 FR 33761 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... research leverages best practices in behavior change, interaction design, and service innovation...

  3. 78 FR 19269 - Medicare and Medicaid Programs; Announcement of Application From a Hospital Requesting Waiver for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    ...A hospital has requested a waiver of statutory requirements that would otherwise require the hospital to enter into an agreement with its designated Organ Procurement Organization (OPO). The request was made in accordance with section 1138(a)(2) of the Social Security Act (the Act). This notice requests comments from OPOs and the general public for our consideration in determining whether we......

  4. 75 FR 5599 - Medicare and Medicaid Programs; Announcement of Applications From Hospitals Requesting Waiver for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-03

    ...Two hospitals have requested waivers of statutory requirements that would otherwise require the hospitals to enter into an agreement with their designated Organ Procurement Organization (OPO). Both requests were made in accordance with section 1138(a)(2) of the Social Security Act (the Act). This notice requests comments from OPOs and the general public for our consideration in determining......

  5. DNA Strand Damage Product Analysis Provides Evidence that the Tumor Cell-Specific Cytotoxin Tirapazamine Produces Hydroxyl Radical and Acts as a Surrogate for O2

    PubMed Central

    Chowdhury, Goutam; Junnotula, Venkatraman; Daniels, J. Scott; Greenberg, Marc M.; Gates, Kent S.

    2010-01-01

    The compound 3-amino-1,2,4-benzotriazine 1,4-dioxide (tirapazamine, TPZ) is a clinically-promising anticancer agent that selectively kills the oxygen-poor (hypoxic) cells found in solid tumors. It has long been known that, under hypoxic conditions, TPZ causes DNA strand damage that is initiated by the abstraction of hydrogen atoms from the deoxyribose phosphate backbone of duplex DNA but exact chemical mechanisms underlying this process remain unclear. Here we describe detailed characterization of sugar-derived products arising from TPZ-mediated strand damage. We find that the action of TPZ on duplex DNA under hypoxic conditions generates 5-methylene-2-furanone (6), oligonucleotide 3′-phosphoglycolates (7), malondialdehyde equivalents (8 or 9), and furfural (10). These results provide evidence that TPZ-mediated strand damage arises via hydrogen atom abstraction from both the most hindered (C1′) and least hindered (C4′ and C5′) positions of the deoxyribose sugars in the double helix. The products observed are identical to those produced by hydroxyl radical. Additional experiments were conducted to better understand the chemical pathways by which TPZ generates the observed DNA-damage products. Consistent with previous work showing that TPZ can substitute for molecular oxygen in DNA damage reactions, it is found that, under anaerobic conditions, reaction of TPZ with a discrete, photogenerated C1′-radical in a DNA 2′-oligodeoxynucleotide cleanly generates the 2-deoxyribonolactone lesion (5) that serves as the precursor to 5-methylene-2-furanone (6). Overall, the results provide insight regarding the chemical structure of the DNA lesions that confront cellular repair, transcription, and replication machinery following exposure to TPZ and offer new information relevant to the chemical mechanisms underlying TPZ-mediated strand cleavage. PMID:17900117

  6. By Request.

    ERIC Educational Resources Information Center

    Beales, Janet R.

    1995-01-01

    Public schools that contract with someone outside to offer an instructional program need to issue a Request for Proposal (RFP) to potential providers. This document details the type of service desired and invites individuals or companies to submit bids or proposals for consideration. Explains the key components of an RFP. (MLF)

  7. DisA and c-di-AMP act at the intersection between DNA-damage response and stress homeostasis in exponentially growing Bacillus subtilis cells.

    PubMed

    Gándara, Carolina; Alonso, Juan C

    2015-03-01

    Bacillus subtilis contains two vegetative diadenylate cyclases, DisA and CdaA, which produce cyclic di-AMP (c-di-AMP), and one phosphodiesterase, GdpP, that degrades it into a linear di-AMP. We report here that DisA and CdaA contribute to elicit repair of DNA damage generated by alkyl groups and H2O2, respectively, during vegetative growth. disA forms an operon with radA (also termed sms) that encodes a protein distantly related to RecA. Among different DNA damage agents tested, only methyl methane sulfonate (MMS) affected disA null strain viability, while radA showed sensitivity to all of them. A strain lacking both disA and radA was as sensitive to MMS as the most sensitive single parent (epistasis). Low c-di-AMP levels (e.g. by over-expressing GdpP) decreased the ability of cells to repair DNA damage caused by MMS and in less extent by H2O2, while high levels of c-di-AMP (absence of GdpP or expression of sporulation-specific diadenylate cyclase, CdaS) increased cell survival. Taken together, our results support the idea that c-di-AMP is a crucial signalling molecule involved in DNA repair with DisA and CdaA contributing to modulate different DNA damage responses during exponential growth. PMID:25616256

  8. Nuclear respiratory factor 2 induces the expression of many but not all human proteins acting in mitochondrial DNA transcription and replication.

    PubMed

    Bruni, Francesco; Polosa, Paola Loguercio; Gadaleta, Maria Nicola; Cantatore, Palmiro; Roberti, Marina

    2010-02-01

    In mammals, NRF-2 (nuclear respiratory factor 2), also named GA-binding protein, is an Ets family transcription factor that controls many genes involved in cell cycle progression and protein synthesis as well as in mitochondrial biogenesis. In this paper, we analyzed the role of NRF-2 in the regulation of human genes involved in mitochondrial DNA transcription and replication. By a combination of bioinformatic and biochemical approaches, we found that the factor binds in vitro and in vivo to the proximal promoter region of the genes coding for the transcription termination factor mTERF, the RNA polymerase POLRMT, the B subunit of the DNA polymerase-gamma, the DNA helicase TWINKLE, and the single-stranded DNA-binding protein mtSSB. The role of NRF-2 in modulating the expression of those genes was further established by RNA interference and overexpression strategies. On the contrary, we found that NRF-2 does not control the genes for the subunit A of DNA polymerase-gamma and for the transcription repressor MTERF3; we suggest that these genes are under regulatory mechanisms that do not involve NRF proteins. Since NRFs are known to positively control the expression of transcription-activating proteins, the novelty emerging from our data is that proteins playing antithetical roles in mitochondrial DNA transcription, namely activators and repressors, are under different regulatory pathways. Finally, we developed a more stringent consensus with respect to the general consensus of NRF-2/GA-binding protein when searching for NRF-2 binding sites in the promoter of mitochondrial proteins. PMID:19951946

  9. 77 FR 58398 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-20

    ...: Title IV-E Plan for Foster Care, Adoption Assistance, and, optional, Guardianship Assistance Programs... Social Security Act (the Act) for each public child welfare agency requesting Federal funding for foster care, adoption assistance and guardianship assistance under the Act. Section 479B of the Act...

  10. 17 CFR 200.304 - Disclosure of requested records.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Counsel, whose zone of responsibility relates to the record requested (see 17 CFR 200.13 et seq.), may..., Public Utility Holding Company Act of 1935, 49 Stat. 833; sec. 319, Trust Indenture Act of 1939, 53 Stat. 1173; sec. 38, Investment Company Act of 1940, 54 Stat. 841; sec. 211, Investment Advisers Act of...

  11. 43 CFR 2.10 - Where do I send my request?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... INFORMATION ACT Requests for Records under the FOIA § 2.10 Where do I send my request? (a) DOI does not have a... of documents in its possession. DOI's files are decentralized and are maintained by various...

  12. 76 FR 24057 - Agency Information Collection Activities; Proposed Collection; Comments Requested; ARCOS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... Information Collection Activities; Proposed Collection; Comments Requested; ARCOS Transaction Reporting DEA...), Drug Enforcement Administration (DEA) will be submitting the following information collection request.... Gallagher, Acting Chief, Liaison and Policy Section, Office of Diversion Control, Drug...

  13. 78 FR 73583 - Request for Comments Concerning Compliance With Telecommunications Trade Agreements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-06

    ... Agama, Acting Chair, Trade Policy Staff Committee. BILLING CODE 3290-F4-P ... TRADE REPRESENTATIVE Request for Comments Concerning Compliance With Telecommunications Trade Agreements AGENCY: Office of the United States Trade Representative. ACTION: Notice of request for public...

  14. 77 FR 37374 - Notice of Request for Revision of Currently Approved Information Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-21

    ... Commodity Credit Corporation Notice of Request for Revision of Currently Approved Information Collection AGENCY: Commodity Credit Corporation, USDA. ACTION: Notice and request for comments. SUMMARY: In accordance with the Paperwork Reduction Act of 1995, this notice announces the Commodity Credit...

  15. 77 FR 37375 - Notice of Request for Extension of Currently Approved Information Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-21

    ... Commodity Credit Corporation Notice of Request for Extension of Currently Approved Information Collection AGENCY: Commodity Credit Corporation, USDA. ACTION: Notice and request for comments. SUMMARY: In accordance with the Paperwork Reduction Act of 1995, this notice announces the Commodity Credit...

  16. 14 CFR 1212.201 - Determining existence of records subject to the Privacy Act.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Act (FOIA) request under 14 CFR part 1206. If the records requested are subject to release under FOIA... processed under this part. NASA will not rely on exemptions contained in FOIA to withhold any record which... ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Requests for Access to Records § 1212.201 Determining existence...

  17. A steroid receptor coactivator acts as the DNA-binding partner of the methoprene-tolerant protein in regulating juvenile hormone response genes.

    PubMed

    Li, Meng; Liu, Pengcheng; Wiley, Jessica D; Ojani, Reyhaneh; Bevan, David R; Li, Jianyong; Zhu, Jinsong

    2014-08-25

    Methoprene-tolerant (Met) protein is a juvenile hormone (JH) receptor in insects. JH-bound Met forms a complex with the βFtz-F1-interacting steroid receptor coactivator (FISC) and together they regulate JH response genes in mosquitoes. Both proteins contain basic helix-loop-helix (bHLH) and PAS motifs. Here we demonstrated that FISC is the obligatory partner of Met for binding to JH-response elements (JHREs). Met or FISC alone could not bind a previously characterized JHRE, while formation of the Met-FISC complex was necessary and sufficient to bind to the JHRE. This binding required participation of the DNA-binding domains of both Met and FISC. The optimal DNA sequence recognized by Met and FISC contained a core consensus sequence GCACGTG. While formation of the Met-FISC complex in mosquito cells was induced by JH, heterodimerization and DNA binding of bacterially expressed Met and FISC were JH-independent, implying that additional mosquito proteins were required to modulate formation of the receptor complex. PMID:25004255

  18. A Steroid Receptor Coactivator Acts as the DNA-binding Partner of the Methoprene-tolerant Protein in Regulating Juvenile Hormone Response Genes

    PubMed Central

    Li, Meng; Liu, Pengcheng; Wiley, Jessica D.; Ojani, Reyhaneh; Bevan, David R.; Li, Jianyong; Zhu, Jinsong

    2014-01-01

    Methoprene-tolerant (Met) protein is a juvenile hormone (JH) receptor in insects. JH-bound Met forms a complex with the βFtz-F1-interacting steroid receptor coactivator (FISC) and together they regulate JH response genes in mosquitoes. Both proteins contain basic-helix-loop-helix (bHLH) and PAS motifs. Here we demonstrated that FISC is the obligatory partner of Met for binding to JH-response elements (JHREs). Met or FISC alone could not bind a previously characterized JHRE, while formation of the Met-FISC complex was necessary and sufficient to bind to the JHRE. This binding required participation of the DNA-binding domains of both Met and FISC. The optimal DNA sequence recognized by Met and FISC contained a core consensus sequence GCACGTG. While formation of the Met-FISC complex in mosquito cells was induced by JH, heterodimerization and DNA binding of bacterially expressed Met and FISC were JH-independent, implying that additional mosquito proteins were required to modulate formation of the receptor complex. PMID:25004255

  19. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act, 5... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the...

  20. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act Requests... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act, 5... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the...

  1. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act, 5... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the requester... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act...

  2. 32 CFR 1901.21 - Processing requests for access to or amendment of records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requirements of 32 CFR 1901.11 through 1901.13 shall be processed under both the Freedom of Information Act, 5... time from a requester pursuant to 32 CFR 1901.33. In such event, the Agency will inform the requester... CENTRAL INTELLIGENCE AGENCY PUBLIC RIGHTS UNDER THE PRIVACY ACT OF 1974 Action on Privacy Act...

  3. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  4. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  5. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  6. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  7. Viability of the antigen determines whether DNA or urocanic acid act as initiator molecules for UV-induced suppression of delayed-type hypersensitivity.

    PubMed

    Kim, Tae-Hueng; Moodycliffe, Angus M; Yarosh, Daniel B; Norval, Mary; Kripke, Margaret L; Ullrich, Stephen E

    2003-09-01

    UV radiation suppresses the immune response, and UV-induced immune suppression contributes to UV-induced photocarcinogenesis. For UV-induced immune suppression to occur, electromagnetic energy (i.e. UV radiation) must be converted to a biological signal. Two photoreceptors have been identified in the skin that serves this purpose, epidermal DNA and trans-urocanic acid (UCA). Although compelling evidence exists to support a role for each pathway (UV-induced DNA damage or photoisomerization of UCA) in UV-induced immune suppression, it is not clear what determines which photoreceptor pathway is activated. To address this question, we injected UV-irradiated mice with a monoclonal antibody with specificity for cis-UCA or applied liposomes containing DNA repair enzymes to the skin of UV-irradiated mice. The effect that each had on UV-induced suppression of delayed-type hypersensitivity was measured. We asked whether the light source used (FS-40 sunlamps vs solar-simulated UV radiation) altered whichever pathway of immune suppression was activated. Different doses of UV radiation and the viability of the antigen were also considered. Neither the dose of UV nor the light source had any influence on determining which pathway was activated. Rather, we found that the viability of the antigen was the critical determinant. When live antigens were used, UV-induced immune suppression was blocked with monoclonal anti-cis-UCA but not with T4 endonuclease V-containing liposomes. The reverse was observed when formalin-fixed or killed antigens were used. Our findings indicate that antigen viability dictates which photoreceptor pathway predominates after UV exposure. PMID:14556308

  8. Residue Substitutions Near the Redox Center of Bacillus subtilis Spx Affect RNA Polymerase Interaction, Redox Control, and Spx-DNA Contact at a Conserved cis-Acting Element

    PubMed Central

    Lin, Ann A.; Walthers, Don

    2013-01-01

    Spx, a member of the ArsC protein family, is a regulatory factor that interacts with RNA polymerase (RNAP). It is highly conserved in Gram-positive bacteria and controls transcription on a genome-wide scale in response to oxidative stress. The structural requirements for RNAP interaction and promoter DNA recognition by Spx were examined through mutational analysis. Residues near the CxxC redox disulfide center of Spx functioned in RNAP α subunit interaction and in promoter DNA binding. R60E and C10A mutants were shown previously to confer defects in transcriptional activation, but both were able to interact with RNAP. R92, which is conserved in ArsC-family proteins, is likely involved in redox control of Spx, as the C10A mutation, which blocks disulfide formation, was epistatic to the R92A mutation. The R91A mutation reduced transcriptional activation and repression, suggesting a defect in RNAP interaction, which was confirmed by interaction assays using an epitope-tagged mutant protein. Protein-DNA cross-linking detected contact between RNAP-bound Spx and the AGCA element at −44 that is conserved in Spx-controlled genes. This interaction caused repositioning of the RNAP σA subunit from a −35-like element upstream of the trxB (thioredoxin reductase) promoter to positions −36 and −11 of the core promoter. The study shows that RNAP-bound Spx contacts a conserved upstream promoter sequence element when bound to RNAP. PMID:23813734

  9. Isolation of cis-acting vaccinia virus DNA fragments promoting the expression of herpes simplex virus thymidine kinase by recombinant viruses.

    PubMed Central

    Vassef, A; Mars, M; Dru, A; Plucienniczak, A; Streeck, R E; Beaud, G

    1985-01-01

    Recombinant TK- vaccinia viruses containing the pBR322 sequence inserted in either orientation within the coding sequence of the viral thymidine kinase gene were constructed. They were characterized by genomic analysis, hybridization studies, reversion to wild-type virus by in vivo recombination, and rescue from their genomes of plasmids which contained all or parts of the pBR322 sequence. TK- cells were infected with one of these recombinant viruses and then transfected with pools of chimeric plasmids composed of a cloned herpes simplex virus thymidine kinase gene which contained upstream inserts of different vaccinia DNA fragments prepared by restriction or sonication. Recombination between homologous pBR322 sequences within infected cells generated selectable recombinant viruses in which expression of the herpes simplex virus thymidine kinase gene was promoted by the upstream vaccinia insert. These viruses were characterized by genomic analysis, hybridization, and in vivo or in vitro phosphorylation of (5-[125I]deoxycytidine as a specific assay for the expressed herpes simplex virus thymidine kinase. Vaccinia DNA inserts were isolated conveniently for transfer to bacteria by rescuing appropriate plasmids from the genome of recombinant viruses. The sequence of 100 nucleotides adjacent to the upstream region of the herpes simplex virus gene was determined in nine different inserts measuring 0.17 to 1.07 kilobase pairs. Images PMID:2989553

  10. 78 FR 13472 - Freedom of Information Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... USC 552, as amended; EO 13392, ``Improving Agency Disclosure of Information,'' 70 FR 75373 (Dec. 19... CFR Part 4 Freedom of Information Act AGENCY: Federal Trade Commission (FTC). ACTION: Final rule... information for the filing of initial Freedom of Information Act (``FOIA'') requests; clarifying...

  11. 77 FR 57013 - Privacy Act; Implementation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... of the Secretary 32 CFR Part 319 Privacy Act; Implementation AGENCY: Defense Intelligence Agency, DoD. ACTION: Direct final rule with request for comments. SUMMARY: The Defense Intelligence Agency is updating the Defense Intelligence Agency Privacy Act Program, by adding the (k)(2) exemption to...

  12. 76 FR 1470 - Sunshine Act Notice

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ... From the Federal Register Online via the Government Publishing Office OVERSEAS PRIVATE INVESTMENT CORPORATION Sunshine Act Notice OPIC Annual Public Hearing OPIC's Sunshine Act notice of its Annual Public Hearing was published in the Federal Register (Volume 75, Number 236, Page 76758) on December 9, 2010. No requests were received to...

  13. 78 FR 54643 - Proposed Information Collection Request; Comment Request; Laboratory Quality Assurance Evaluation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ...The Environmental Protection Agency is planning to submit an information collection request (ICR), ``Laboratory Quality Assurance Evaluation Program for Analysis of Cryptosporidium Under the Safe Drinking Water Act'' (EPA ICR No. 2067.05, OMB Control No. 2040-0246) to the Office of Management and Budget (OMB) for review and approval in accordance with the Paperwork Reduction Act (44 U.S.C.......

  14. 75 FR 9992 - Agency Information Collection Activities: Proposed Request and Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-04

    ... December 28, 2009 at 74 FR 68655. The correct information is below. Type of Request: Revision of an OMB... December 28, 2009 at 74 FR 68655. In addition, since we are revising the Privacy Act Statement, this is now... at 74 FR 64801. Since we are revising the Privacy Act Statement, this is now a revision of an...

  15. 75 FR 30368 - Proposed Information Collection; Comment Request; Procedures for Considering Requests and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-01

    ... Act, and for providing relief under section 322(b) of the Act. In Proclamation No. 8341 (74 FR 4103... Requests and Comments From the Public for Textile and Apparel Safeguard Actions on Imports From Peru AGENCY... Carrigg, Office of Textiles and Apparel, U.S. Department of Commerce, Telephone: 202-482-2573, Fax:...

  16. 77 FR 31832 - Proposed Information Collection; Comment Request; Procedures for Considering Requests and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-30

    ... Act, and for providing relief under section 322(b) of the Act. In Proclamation No. 8818 (77 FR 29519... Requests and Comments From the Public for Textile and Apparel Safeguard Actions on Imports From Colombia... to Robert Carrigg, Office of Textiles and Apparel, U.S. Department of Commerce, Telephone:...

  17. 78 FR 48142 - Proposed Information Collection; Comment Request; Procedures for Considering Requests and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-07

    ... Act, and for providing relief under section 322(b) of the Act. In Proclamation No. 8341 (74 FR 4105... Requests and Comments From the Public Under the Textile Safeguard Provision of the United States-Peru Free... instructions should be directed to Laurie Mease, Office of Textiles and Apparel, U.S. Department of...

  18. Collaborative Dialogue in Learning Pragmatics: Pragmatic-Related Episodes as an Opportunity for Learning Request-Making

    ERIC Educational Resources Information Center

    Taguchi, Naoko; Kim, Youjin

    2016-01-01

    This study examined the effects of collaborative dialogue in learning the speech act of request. Seventy-four second-grade girls' junior high students were divided into three groups. The "collaborative group" (n = 25) received explicit metapragmatic information on request (request head act and modifications) followed by a dialogue…

  19. 19 CFR 201.29 - Commission disclosure of individual records, accounting of record disclosures, and requests for...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... disclosure required by 5 U.S.C. 552, the Privacy Act Officer shall keep an accurate accounting of: (1) The... individual requesting an accounting of disclosure of his or her records should make the request in writing to... and in the letter that it is a Privacy Act request for an accounting of disclosure of records....

  20. 78 FR 76859 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Prevent All...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... Collection; Comments Requested: Prevent All Cigarette Trafficking (PACT) Act Registration Form ACTION: 60-Day... a currently approved collection. (2) The title of the form/collection: Prevent All...

  1. The Drosophila melanogaster DNA Ligase IV gene plays a crucial role in the repair of radiation-induced DNA double-strand breaks and acts synergistically with Rad54.

    PubMed Central

    Gorski, Marcin M; Eeken, Jan C J; de Jong, Anja W M; Klink, Ilse; Loos, Marjan; Romeijn, Ron J; van Veen, Bert L; Mullenders, Leon H; Ferro, Wouter; Pastink, Albert

    2003-01-01

    DNA Ligase IV has a crucial role in double-strand break (DSB) repair through nonhomologous end joining (NHEJ). Most notably, its inactivation leads to embryonic lethality in mammals. To elucidate the role of DNA Ligase IV (Lig4) in DSB repair in a multicellular lower eukaryote, we generated viable Lig4-deficient Drosophila strains by P-element-mediated mutagenesis. Embryos and larvae of mutant lines are hypersensitive to ionizing radiation but hardly so to methyl methanesulfonate (MMS) or the crosslinking agent cis-diamminedichloroplatinum (cisDDP). To determine the relative contribution of NHEJ and homologous recombination (HR) in Drosophila, Lig4; Rad54 double-mutant flies were generated. Survival studies demonstrated that both HR and NHEJ have a major role in DSB repair. The synergistic increase in sensitivity seen in the double mutant, in comparison with both single mutants, indicates that both pathways partially overlap. However, during the very first hours after fertilization NHEJ has a minor role in DSB repair after exposure to ionizing radiation. Throughout the first stages of embryogenesis of the fly, HR is the predominant pathway in DSB repair. At late stages of development NHEJ also becomes less important. The residual survival of double mutants after irradiation strongly suggests the existence of a third pathway for the repair of DSBs in Drosophila. PMID:14704177

  2. Inhibitor of Differentiation 4 (ID4) Acts as an Inhibitor of ID-1, -2 and -3 and Promotes basic Helix Loop Helix (bHLH) E47 DNA Binding and Transcriptional Activity

    PubMed Central

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-01-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID- interactions. PMID:25778840

  3. Inhibitor of differentiation 4 (ID4) acts as an inhibitor of ID-1, -2 and -3 and promotes basic helix loop helix (bHLH) E47 DNA binding and transcriptional activity.

    PubMed

    Sharma, Pankaj; Chinaranagari, Swathi; Chaudhary, Jaideep

    2015-05-01

    The four known ID proteins (ID1-4, Inhibitor of Differentiation) share a homologous helix loop helix (HLH) domain and act as dominant negative regulators of basic-HLH transcription factors. ID proteins also interact with many non-bHLH proteins in complex networks. The expression of ID proteins is increasingly observed in many cancers. Whereas ID-1, ID-2 and ID-3, are generally considered as tumor promoters, ID4 on the contrary has emerged as a tumor suppressor. In this study we demonstrate that ID4 heterodimerizes with ID-1, -2 and -3 and promote bHLH DNA binding, essentially acting as an inhibitor of inhibitors of differentiation proteins. Interaction of ID4 was observed with ID1, ID2 and ID3 that was dependent on intact HLH domain of ID4. Interaction with bHLH protein E47 required almost 3 fold higher concentration of ID4 as compared to ID1. Furthermore, inhibition of E47 DNA binding by ID1 was restored by ID4 in an EMSA binding assay. ID4 and ID1 were also colocalized in prostate cancer cell line LNCaP. The alpha helix forming alanine stretch N-terminal, unique to HLH ID4 domain was required for optimum interaction. Ectopic expression of ID4 in DU145 prostate cancer line promoted E47 dependent expression of CDKNI p21. Thus counteracting the biological activities of ID-1, -2 and -3 by forming inactive heterodimers appears to be a novel mechanism of action of ID4. These results could have far reaching consequences in developing strategies to target ID proteins for cancer therapy and understanding biologically relevant ID-interactions. PMID:25778840

  4. 20 CFR 402.15 - Relationship between the FOIA and the Privacy Act of 1974.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... “Individuals” and “system of records” are defined in the Privacy Act and in 20 CFR 401.25. (b) Requesting your... defined in the Privacy Act and in 20 CFR 401.25.) If we handle your request under the FOIA and the rules... Act of 1974. 402.15 Section 402.15 Employees' Benefits SOCIAL SECURITY ADMINISTRATION AVAILABILITY...

  5. 5 CFR 1205.22 - Action on request.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Action on request. 1205.22 Section 1205.22 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES PRIVACY ACT REGULATIONS Amendment of Records § 1205.22 Action on request. (a) Amendment granted. If the Board grants...

  6. 5 CFR 9301.5 - Accessing records without request.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Accessing records without request. 9301.5 Section 9301.5 Administrative Personnel SPECIAL INSPECTOR GENERAL FOR AFGHANISTAN RECONSTRUCTION... the Freedom of Information Act § 9301.5 Accessing records without request. Certain SIGAR...

  7. 6 CFR 5.5 - Timing of responses to requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Timing of responses to requests. 5.5 Section 5.5 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act § 5.5 Timing of responses to requests. (a) In general....

  8. 6 CFR 5.5 - Timing of responses to requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Timing of responses to requests. 5.5 Section 5.5 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act § 5.5 Timing of responses to requests. (a) In general....

  9. 6 CFR 5.5 - Timing of responses to requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Timing of responses to requests. 5.5 Section 5.5 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act § 5.5 Timing of responses to requests. (a) In general....

  10. 40 CFR 1602.2 - Requests for access to records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 33 2011-07-01 2011-07-01 false Requests for access to records. 1602.2 Section 1602.2 Protection of Environment CHEMICAL SAFETY AND HAZARD INVESTIGATION BOARD PROTECTION OF PRIVACY AND ACCESS TO INDIVIDUAL RECORDS UNDER THE PRIVACY ACT OF 1974 § 1602.2 Requests for access...

  11. 32 CFR 1662.3 - Identification of information requested.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Identification of information requested. 1662.3 Section 1662.3 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM FREEDOM OF INFORMATION ACT (FOIA) PROCEDURES § 1662.3 Identification of information requested. Any...

  12. 32 CFR 1662.3 - Identification of information requested.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Identification of information requested. 1662.3 Section 1662.3 National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM FREEDOM OF INFORMATION ACT (FOIA) PROCEDURES § 1662.3 Identification of information requested. Any...

  13. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  14. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  15. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  16. 5 CFR 1630.17 - Federal agency requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Federal agency requests. 1630.17 Section 1630.17 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD PRIVACY ACT REGULATIONS § 1630.17 Federal agency requests. Employing agencies needing automated data processing services from...

  17. 5 CFR 1630.17 - Federal agency requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Federal agency requests. 1630.17 Section 1630.17 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD PRIVACY ACT REGULATIONS § 1630.17 Federal agency requests. Employing agencies needing automated data processing services from...

  18. 5 CFR 1630.17 - Federal agency requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Federal agency requests. 1630.17 Section 1630.17 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD PRIVACY ACT REGULATIONS § 1630.17 Federal agency requests. Employing agencies needing automated data processing services from...

  19. 5 CFR 1630.17 - Federal agency requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Federal agency requests. 1630.17 Section 1630.17 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD PRIVACY ACT REGULATIONS § 1630.17 Federal agency requests. Employing agencies needing automated data processing services from...

  20. 32 CFR 1900.21 - Processing of requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... requester pursuant to 32 CFR 1900.33. In such event, the Agency will inform the requester in writing and... AGENCY PUBLIC ACCESS TO CIA RECORDS UNDER THE FREEDOM OF INFORMATION ACT (FOIA) Cia Action on Foia... taskings to the CIA components reasonably believed to hold responsive records. Effective October 2,...