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Sample records for act request dna

  1. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  2. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  3. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  4. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  5. 32 CFR Appendix A to Part 291 - Freedom of Information Act Request (DNA Form 524)

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Freedom of Information Act Request (DNA Form 524... OF DEFENSE (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE NUCLEAR AGENCY (DNA) FREEDOM OF INFORMATION ACT PROGRAM Pt. 291, App. A Appendix A to Part 291—Freedom of Information Act Request (DNA...

  6. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Privacy Act requests by inmates. 513.50... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR...

  7. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Privacy Act requests by inmates. 513.50... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR...

  8. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Privacy Act requests by inmates. 513.50... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR...

  9. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Privacy Act requests by inmates. 513.50... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR...

  10. 28 CFR 513.50 - Privacy Act requests by inmates.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Privacy Act requests by inmates. 513.50... ADMINISTRATION ACCESS TO RECORDS Release of Information Privacy Act Requests for Information § 513.50 Privacy Act requests by inmates. Because inmate records are exempt from disclosure under the Privacy Act (see 28 CFR...

  11. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 1 2012-10-01 2012-10-01 false FOIA and Privacy Act requests. 0.506 Section 0... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for....461), of the Privacy Act of 1974, (See § 0.554) shall be processed in accordance with the...

  12. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 1 2011-10-01 2011-10-01 false FOIA and Privacy Act requests. 0.506 Section 0... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for....461), of the Privacy Act of 1974, (See § 0.554) shall be processed in accordance with the...

  13. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 1 2013-10-01 2013-10-01 false FOIA and Privacy Act requests. 0.506 Section 0... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for....461), of the Privacy Act of 1974, (See § 0.554) shall be processed in accordance with the...

  14. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT) Requests for Access or Amendment § 1008.6 Procedures for Privacy Act requests. (a) Any individual may— (1)...

  15. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false FOIA and Privacy Act requests. 0.506 Section 0... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for....461), of the Privacy Act of 1974, (See § 0.554) shall be processed in accordance with the...

  16. 47 CFR 0.506 - FOIA and Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 1 2014-10-01 2014-10-01 false FOIA and Privacy Act requests. 0.506 Section 0... Declassification of National Security Information § 0.506 FOIA and Privacy Act requests. Requests for....461), of the Privacy Act of 1974, (See § 0.554) shall be processed in accordance with the...

  17. 6 CFR 1002.3 - Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Privacy Act requests. 1002.3 Section 1002.3 Domestic Security PRIVACY AND CIVIL LIBERTIES OVERSIGHT BOARD IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 1002.3 Privacy Act requests. (a) Requests to determine if you are the subject of a record. You...

  18. 6 CFR 1002.4 - Responses to Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Responses to Privacy Act requests. 1002.4 Section 1002.4 Domestic Security PRIVACY AND CIVIL LIBERTIES OVERSIGHT BOARD IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 1002.4 Responses to Privacy Act requests. (a) Acknowledgement. The Privacy Act...

  19. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY... Act requests. The Office of Science and Technology Policy shall process requests for...

  20. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY... Act requests. The Office of Science and Technology Policy shall process requests for...

  1. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY... Act requests. The Office of Science and Technology Policy shall process requests for...

  2. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY... Act requests. The Office of Science and Technology Policy shall process requests for...

  3. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... pursuant to this section may be obtained from any DOE Privacy Act Officer at the locations listed at § 1008... 10 Energy 4 2014-01-01 2014-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT)...

  4. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... pursuant to this section may be obtained from any DOE Privacy Act Officer at the locations listed at § 1008... 10 Energy 4 2011-01-01 2011-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT)...

  5. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... pursuant to this section may be obtained from any DOE Privacy Act Officer at the locations listed at § 1008... 10 Energy 4 2012-01-01 2012-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT)...

  6. 10 CFR 1008.6 - Procedures for Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... pursuant to this section may be obtained from any DOE Privacy Act Officer at the locations listed at § 1008... 10 Energy 4 2013-01-01 2013-01-01 false Procedures for Privacy Act requests. 1008.6 Section 1008.6 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) RECORDS MAINTAINED ON INDIVIDUALS (PRIVACY ACT)...

  7. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) A Appendix A to Part 2507 Public Welfare Regulations Relating to Public Welfare (Continued... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter (Sample... Information Act Request. Dear____: This is a request under the Freedom of Information Act. I request that...

  8. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) A Appendix A to Part 2507 Public Welfare Regulations Relating to Public Welfare (Continued... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter (Sample... Information Act Request. Dear____: This is a request under the Freedom of Information Act. I request that...

  9. 45 CFR Appendix A to Part 2507 - Freedom of Information Act Request Letter (Sample)

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) A Appendix A to Part 2507 Public Welfare Regulations Relating to Public Welfare (Continued... INFORMATION ACT Pt. 2507, App. A Appendix A to Part 2507—Freedom of Information Act Request Letter (Sample... Information Act Request. Dear____: This is a request under the Freedom of Information Act. I request that...

  10. Request for Correction 15003 - Information Quality Act request for Correction of the TSCA Work Plan

    EPA Pesticide Factsheets

    This RFC concerns the Information Quality Act request for correction of the TSCA Work Plan for Chemical Assessments: 20154 Update and the TSCA Work Plan Chemicals: Methods Document (February 2012) regarding assessment of phthalic anhydride

  11. Privacy Act System of Records: Freedom of Information Act Request and Appeal File, EPA-9

    EPA Pesticide Factsheets

    Learn more about the Freedom of Information Act Request and Appeal File System, including who is covered in the system, the purpose of data collection, routine uses for the system's records, and other security procedures.

  12. DNA analysis: what and when to request?

    PubMed Central

    Norbury, G; Norbury, C J

    2006-01-01

    Over the last 15 years genetic testing by DNA analysis has expanded enormously both in volume and range due to advances in scientific knowledge and analytical technology. This type of analysis has the potential to provide rapid, cost effective, and accurate diagnostic information but also has its limitations. Some of the changes detected may be of ambiguous consequence and as the knowledge base expands so too does the recognition that other factors can influence the clinical picture. In many cases outcomes may be predicted only on a statistical basis rather than individually. Careful attention should therefore be given to the clinical question that is being addressed before such testing is requested. PMID:16551793

  13. 78 FR 62629 - Proposed Information Collection Request; Comment Request; Clean Water Act Section 404 State...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ...The Environmental Protection Agency is planning to submit an information collection request (ICR), ``Clean Water Act Section 404 State-Assumed Programs'' (EPA ICR No. 0220.12, OMB Control No. 2040- 0168) to the Office of Management and Budget (OMB) for review and approval in accordance with the Paperwork Reduction Act (44 U.S.C. 3501 et seq.). Before doing so, EPA is soliciting public comments......

  14. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Requirements for making Privacy Act requests. 1830.2 Section 1830.2 Administrative Personnel OFFICE OF SPECIAL COUNSEL PRIVACY § 1830.2 Requirements for making Privacy Act requests. (a) How made and addressed. A request for OSC records under...

  15. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Requirements for making Privacy Act requests. 1830.2 Section 1830.2 Administrative Personnel OFFICE OF SPECIAL COUNSEL PRIVACY § 1830.2 Requirements for making Privacy Act requests. (a) How made and addressed. A request for OSC records under...

  16. 5 CFR 1830.2 - Requirements for making Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Requirements for making Privacy Act requests. 1830.2 Section 1830.2 Administrative Personnel OFFICE OF SPECIAL COUNSEL PRIVACY § 1830.2 Requirements for making Privacy Act requests. (a) How made and addressed. A request for OSC records under...

  17. 78 FR 32227 - Perishable Agricultural Commodities Act; Request for Extension and Revision of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-29

    ... Agricultural Marketing Service Perishable Agricultural Commodities Act; Request for Extension and Revision of a Currently Approved Information Collection AGENCY: Agricultural Marketing Service, USDA. ACTION: Notice and... 35), this document announces the Agricultural Marketing Service's (AMS) intention to request...

  18. Summary of Waiver Requests Under Section 211(f) of the Clean Air Act

    EPA Pesticide Factsheets

    This document summarizes the waiver requests that EPA has received under Section 211(f) of the Clean Air Act, including docket numbers, federal register citations, and the actions that were taken by EPA for each request.

  19. 78 FR 23885 - Federal Seed Act Program; Request for Extension and Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-23

    ... Agricultural Marketing Service Federal Seed Act Program; Request for Extension and Revision of a Currently Approved Information Collection AGENCY: Agricultural Marketing Service, USDA. ACTION: Request for comments... announces the Agricultural Marketing Service's (AMS) intention to request approval, from the Office...

  20. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Maintenance of Meeting Records § 1703.403 Requests for records under Freedom of Information and Privacy Acts... 45 Public Welfare 4 2011-10-01 2011-10-01 false Requests for records under Freedom of...

  1. 45 CFR 1703.403 - Requests for records under Freedom of Information and Privacy Acts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... (Continued) NATIONAL COMMISSION ON LIBRARIES AND INFORMATION SCIENCE GOVERNMENT IN THE SUNSHINE ACT Maintenance of Meeting Records § 1703.403 Requests for records under Freedom of Information and Privacy Acts... 45 Public Welfare 4 2010-10-01 2010-10-01 false Requests for records under Freedom of...

  2. Effects of Culture and Gender in Comprehension of Speech Acts of Indirect Request

    ERIC Educational Resources Information Center

    Shams, Rabe'a; Afghari, Akbar

    2011-01-01

    This study investigates the comprehension of indirect request speech act used by Iranian people in daily communication. The study is an attempt to find out whether different cultural backgrounds and the gender of the speakers affect the comprehension of the indirect request of speech act. The sample includes thirty males and females in Gachsaran…

  3. 36 CFR 1202.54 - On what grounds can NARA deny my Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false On what grounds can NARA deny... Access to Records § 1202.54 On what grounds can NARA deny my Privacy Act request? (a) NARA can deny your... Information Act (FOIA). (c) Upon receipt of a request for access to a record which is contained within...

  4. 38 CFR 20.1200 - Rule 1200. Privacy Act request-appeal pending.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Rule 1200. Privacy Act... VETERANS AFFAIRS (CONTINUED) BOARD OF VETERANS' APPEALS: RULES OF PRACTICE Privacy Act § 20.1200 Rule 1200. Privacy Act request—appeal pending. When a Privacy Act request is filed under § 1.577 of this chapter...

  5. 38 CFR 20.1200 - Rule 1200. Privacy Act request-appeal pending.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Rule 1200. Privacy Act... VETERANS AFFAIRS (CONTINUED) BOARD OF VETERANS' APPEALS: RULES OF PRACTICE Privacy Act § 20.1200 Rule 1200. Privacy Act request—appeal pending. When a Privacy Act request is filed under § 1.577 of this chapter...

  6. 17 CFR Appendix A to Part 146 - Fees for Copies of Records Requested Under the Privacy Act of 1974

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Requested Under the Privacy Act of 1974 A Appendix A to Part 146 Commodity and Securities Exchanges... 146—Fees for Copies of Records Requested Under the Privacy Act of 1974 a. The following schedule of fees shall apply to copies of records requested pursuant to the Privacy Act of 1974, 5 U.S.C. 552a...

  7. 77 FR 53237 - Submission for Review; Information Collection: Freedom of Information/Privacy Act Record Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-31

    ... MANAGEMENT Submission for Review; Information Collection: Freedom of Information/Privacy Act Record Request... request (ICR), Office of Management and Budget (OMB) Control No. 3206-NEW, Freedom of Information/Privacy... other technological collection techniques or other forms of information technology, e.g.,...

  8. 75 FR 69126 - Proposed Information Collection Request (ICR) for the Workforce Investment Act Random Assignment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-10

    ... Information Collection Request (ICR) for the Workforce Investment Act Random Assignment Impact Evaluation of... a Skilled Workforce--to conduct this evaluation. The evaluation will address the following research...). SUPPLEMENTARY INFORMATION: I. Background In 1998, Congress significantly reformed the public...

  9. 76 FR 58277 - Animal Generic Drug User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-20

    ... HUMAN SERVICES Food and Drug Administration Animal Generic Drug User Fee Act; Public Meeting; Request... comments. The Food and Drug Administration (FDA) is announcing a public meeting on the Animal Generic Drug... on the Internet at...

  10. 76 FR 4745 - Proposed Collection; Comment Request; Bank Secrecy Act Designation of Exempt Person Report...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ... Financial Crimes Enforcement Network Proposed Collection; Comment Request; Bank Secrecy Act Designation of Exempt Person Report Proposed Data Fields AGENCY: Financial Crimes Enforcement Network (``FinCEN... be submitted to: Regulatory Policy and Programs Division, Financial Crimes Enforcement...

  11. 78 FR 24227 - Notice of Proposed Information Collection; Comment Request: Real Estate Settlement Procedures Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... URBAN DEVELOPMENT Notice of Proposed Information Collection; Comment Request: Real Estate Settlement... information: Title of Proposal: Real Estate Settlement Procedures Act (RESPA) Disclosures. OMB Control Number... 24 CFR part 3500, require real estate settlement service providers to give homebuyers...

  12. 75 FR 12496 - Proposed Information Collection; Comment Request; DOC National Environmental Policy Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... a diverse range of potential environmental issues covered under Federal environmental laws and... Office of the Secretary Proposed Information Collection; Comment Request; DOC National Environmental Policy Act Environmental Questionnaire and Checklist AGENCY: Office of the Secretary, Office...

  13. 8 CFR 103.10 - Requests for records under the Freedom of Information Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...; Spokane, WA; and St. Albans, VT. In certain cases, a district director may designate another Service... requester automatically agrees to pay fees up to $25.00 by filing a Freedom of Information Act...

  14. 8 CFR 103.10 - Requests for records under the Freedom of Information Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...; Spokane, WA; and St. Albans, VT. In certain cases, a district director may designate another Service... requester automatically agrees to pay fees up to $25.00 by filing a Freedom of Information Act...

  15. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Freedom of Information Act (FOIA) requests. 600.155 Section 600.155 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS...

  16. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Freedom of Information Act (FOIA) requests. 600.155 Section 600.155 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS...

  17. 50 CFR 600.155 - Freedom of Information Act (FOIA) requests.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Freedom of Information Act (FOIA) requests. 600.155 Section 600.155 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS...

  18. 46 CFR 503.32 - Procedures for responding to requests made under the Freedom of Information Act.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Freedom of Information Act. 503.32 Section 503.32 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS PUBLIC INFORMATION Requests for Records Under the Freedom of Information § 503.32 Procedures for responding to requests made under the Freedom of Information Act. (a) Determination to...

  19. 46 CFR 503.32 - Procedures for responding to requests made under the Freedom of Information Act.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Freedom of Information Act. 503.32 Section 503.32 Shipping FEDERAL MARITIME COMMISSION GENERAL AND ADMINISTRATIVE PROVISIONS PUBLIC INFORMATION Requests for Records Under the Freedom of Information § 503.32 Procedures for responding to requests made under the Freedom of Information Act. (a) Determination to...

  20. 17 CFR Appendix A to Part 146 - Fees for Copies of Records Requested Under the Privacy Act of 1974

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 1 2013-04-01 2013-04-01 false Fees for Copies of Records Requested Under the Privacy Act of 1974 A Appendix A to Part 146 Commodity and Securities Exchanges... 146—Fees for Copies of Records Requested Under the Privacy Act of 1974 a. The following schedule...

  1. 76 FR 33413 - Proposed Renewal Without Change; Comment Request; Nine Bank Secrecy Act Recordkeeping Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-08

    ... Financial Crimes Enforcement Network Proposed Renewal Without Change; Comment Request; Nine Bank Secrecy Act... be submitted to: Financial Crimes Enforcement Network, P.O. Box 39, Vienna, VA 22183, Attention: BSA... Requirements Comments.'' FOR FURTHER INFORMATION CONTACT: Financial Crimes Enforcement Network,...

  2. 75 FR 996 - Medicare Prescription Drug, Improvement, and Modernization Act of 2003 Section 1013: Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-07

    ... Modernization Act of 2003 Section 1013: Request for Nominations--The Effective Health Care Stakeholder Group... nominations for the Effective Health Care Stakeholder Group. SUMMARY: The DHHS Agency for Healthcare Research... members of the Stakeholder Group to support the work of the Effective Health Care Program,...

  3. 75 FR 17771 - Comment Request for Information Collection for Jobs for Veterans Act Priority of Service...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-07

    ... Comment Request for Information Collection for Jobs for Veterans Act Priority of Service Provisions: OMB... information technology, e.g., permitting electronic submissions of responses. III. Current Actions Type of... agencies with an opportunity to comment on proposed and/or continuing collections of information...

  4. 32 CFR 2400.22 - Freedom of Information Act and Privacy Act requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Freedom of Information Act and Privacy Act... SCIENCE AND TECHNOLOGY POLICY REGULATIONS TO IMPLEMENT E.O. 12356; OFFICE OF SCIENCE AND TECHNOLOGY POLICY INFORMATION SECURITY PROGRAM Declassification and Downgrading § 2400.22 Freedom of Information Act and...

  5. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... TRIBAL CLEAN AIR ACT AUTHORITY Tribal Authority § 49.5 Tribal requests for additional Clean Air Act... request that the Administrator specify additional provisions of the Clean Air Act for which it would be... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Tribal requests for additional...

  6. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  7. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  8. 40 CFR 49.7 - Request by an Indian tribe for eligibility determination and Clean Air Act program approval.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... eligibility determination and Clean Air Act program approval. 49.7 Section 49.7 Protection of Environment... MANAGEMENT Tribal Authority § 49.7 Request by an Indian tribe for eligibility determination and Clean Air Act... it meets the eligibility requirements of § 49.6 for Clean Air Act program approval. The...

  9. 77 FR 33388 - Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-06

    ... Animal and Plant Health Inspection Service Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration Requirement; Plants and Plant Products AGENCY: Animal and Plant Health... Plant Health Inspection Service's intention to request an extension of approval of an...

  10. 78 FR 15019 - Food and Drug Administration Prescription Drug User Fee Act V Benefit-Risk Plan; Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... HUMAN SERVICES Food and Drug Administration Food and Drug Administration Prescription Drug User Fee Act V Benefit-Risk Plan; Request for Comments AGENCY: Food and Drug Administration, HHS. ACTION: Notice, request for comments. SUMMARY: The Food and Drug Administration (FDA or the Agency) is announcing...

  11. 42 CFR 137.227 - How much time does the Secretary have to act on a waiver request?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... HUMAN SERVICES INDIAN HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Regulation Waiver § 137.227 How much time does the Secretary have to act on a waiver request? The...

  12. 42 CFR 137.227 - How much time does the Secretary have to act on a waiver request?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... HUMAN SERVICES INDIAN HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Regulation Waiver § 137.227 How much time does the Secretary have to act on a waiver request? The...

  13. 3 CFR - State of California Request for Waiver Under 42 U.S.C. 7543(b), the Clean Air Act

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 3 The President 1 2010-01-01 2010-01-01 false State of California Request for Waiver Under 42 U.S.C. 7543(b), the Clean Air Act Presidential Documents Other Presidential Documents Memorandum of January 26, 2009 State of California Request for Waiver Under 42 U.S.C. 7543(b), the Clean Air Act Memorandum for the Administrator of the...

  14. Small circular DNA molecules act as rigid motifs to build DNA nanotubes.

    PubMed

    Zheng, Hongning; Xiao, Minyu; Yan, Qin; Ma, Yinzhou; Xiao, Shou-Jun

    2014-07-23

    Small circular DNA molecules with designed lengths, for example 64 and 96 nucleotides (nt), after hybridization with a few 32-nt staple strands respectively, can act as rigid motifs for the construction of DNA nanotubes with excellent uniformity in ring diameter. Unlike most native DNA nanotubes, which consist of longitudinal double helices, nanotubes assembled from circular DNAs are constructed from lateral double helices. Of the five types of DNA nanotubes designed here, four are built by alternating two different rings of the same ring size, while one is composed of all the same 96-nt rings. Nanotubes constructed from the same 96-nt rings are 10-100 times shorter than those constructed from two different 96-nt rings, because there are fewer hinge joints on the rings.

  15. 43 CFR 3742.3-1 - Request for publication of notice of Leasing Act filing; supporting instruments.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Request for publication of notice of Leasing Act filing; supporting instruments. 3742.3-1 Section 3742.3-1 Public Lands: Interior Regulations... for publication of notice of Leasing Act filing; supporting instruments. (a) Having complied with...

  16. 40 CFR 1515.5 - Making a Freedom of Information Act request.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... needed to process the request. CEQ may provide requesters on a slower track an opportunity to limit the scope of their request in order to qualify for faster processing. (4) The Council will make a...

  17. DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair

    PubMed Central

    Abdou, Ismail; Poirier, Guy G.; Hendzel, Michael J.; Weinfeld, Michael

    2015-01-01

    In the current model of DNA SSBR, PARP1 is regarded as the sensor of single-strand breaks (SSBs). However, biochemical studies have implicated LIG3 as another possible SSB sensor. Using a laser micro-irradiation protocol that predominantly generates SSBs, we were able to demonstrate that PARP1 is dispensable for the accumulation of different single-strand break repair (SSBR) proteins at sites of DNA damage in live cells. Furthermore, we show in live cells for the first time that LIG3 plays a role in mediating the accumulation of the SSBR proteins XRCC1 and PNKP at sites of DNA damage. Importantly, the accumulation of LIG3 at sites of DNA damage did not require the BRCT domain-mediated interaction with XRCC1. We were able to show that the N-terminal ZnF domain of LIG3 plays a key role in the enzyme's SSB sensing function. Finally, we provide cellular evidence that LIG3 and not PARP1 acts as the sensor for DNA damage caused by the topoisomerase I inhibitor, irinotecan. Our results support the existence of a second damage-sensing mechanism in SSBR involving the detection of nicks in the genome by LIG3. PMID:25539916

  18. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air Act... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Tribal requests for additional...

  19. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air Act... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Tribal requests for additional...

  20. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air Act... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Tribal requests for additional...

  1. 40 CFR 49.5 - Tribal requests for additional Clean Air Act provisions for which it is not appropriate to treat...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... additional Clean Air Act provisions for which it is not appropriate to treat tribes in the same manner as States. Any tribe may request that the Administrator specify additional provisions of the Clean Air Act... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Tribal requests for additional...

  2. Report: Congressionally Requested Report on Comments Related to Effects of Jurisdictional Uncertainty on Clean Water Act Implementation

    EPA Pesticide Factsheets

    Report #09-N-0149, April 30, 2009. This is in response to a request dated April 24, 2009, asking that the EPA OIG provide information we have collected on the impact of the Rapanos case on Clean Water Act (CWA) enforcement.

  3. 28 CFR Appendix to Subpart H - Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Delegation of Authority Respecting Denials of Freedom of Information and Privacy Act Requests Appendix to Subpart H Judicial Administration DEPARTMENT OF JUSTICE ORGANIZATION OF THE DEPARTMENT OF JUSTICE Antitrust Division Pt. 0, Subpt. H,...

  4. 43 CFR 2.27 - How will a bureau handle a request for information that is contained in a Privacy Act system of...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... information that is contained in a Privacy Act system of records? (See DOI's Privacy Act regulations (Subpart... system of records? (See DOI's Privacy Act regulations (Subpart G of this part) for additional information.) (a) When you request information pertaining to yourself that is contained in a Privacy Act system...

  5. 43 CFR 2.27 - How will a bureau handle a request for information that is contained in a Privacy Act system of...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... information that is contained in a Privacy Act system of records? (See DOI's Privacy Act regulations (Subpart... system of records? (See DOI's Privacy Act regulations (Subpart G of this part) for additional information.) (a) When you request information pertaining to yourself that is contained in a Privacy Act system...

  6. 78 FR 38430 - Paperwork Reduction Act of 1995, as Amended; Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-26

    ... Clearance Officer no later than July 26, 2013. SUPPLEMENTARY INFORMATION: Type of Request: Reauthorization. Title of Information Collection: Section 26a Permit Application. Frequency of Use: On occasion. Type of... Authority. ACTION: Proposed Collection; comment request. SUMMARY: The proposed information...

  7. Using the Freedom of Information Act (FOIA) Request Process to Get Information about Risk Management Plans

    EPA Pesticide Factsheets

    FOIAonline allows the public to electronically submit/modify a FOIA request, check its status, search previously submitted requests, view responsive documents that are cleared for release, and file an appeal.

  8. A DNA ring acting as a thermal ratchet.

    PubMed

    Kulić, Igor M; Thaokar, Rochish; Schiessel, Helmut

    2005-11-30

    Several DNA nanomotors have been recently constructed in laboratories worldwide. These machines are, however, relatively slow and do not perform continuous rotations. We have recently proposed a rotary DNA nanomachine that shows a continuous rotation with a frequency of 10(2)-10(4) Hz. This motor is a closed DNA ring whose elastic features are tuned such that it can be externally driven via e.g. periodic temperature changes. As a result, the twirling ring propels itself through the fluid with a speed of tens of nanometres up to a few microns per second. The current paper gives a more detailed presentation of this motor and provides a derivation of the low- and high-frequency asymptotic behaviour of thermal ratchets in general.

  9. 77 FR 72356 - Animal Drug User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... Requests for Oral Presentations: Send registration information (including name, title, firm name, address, telephone, and fax number), and written material and requests to make oral presentations, to the contact... cycles and improve communications during reviews. The ADUFA programs have enabled FDA to speed up...

  10. 77 FR 72359 - Animal Generic Drug User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... . Registration and Requests for Oral Presentations: Send registration information (including name, title, firm name, address, telephone, and fax number), and written material and requests to make oral presentations... enable FDA to speed up the application review process for generic new animal drugs without...

  11. Identification of a UV-induced trans-acting protein that stimulates polyomavirus DNA replication

    SciTech Connect

    Ronai, Z.A.; Weinstein, I.B. )

    1988-03-01

    Previous studies provided indirect evidence that the ability of a variety of DNA-damaging agents to induce asynchronous polyomavirus DNA replication in the H3 rat fibroblast cell line is mediated by a trans-acting factor. Using an erythrocyte insertion technique to introduce protein fractions from UV-irradiated cells into unirradiated H3 cells, we have now obtained evidence that this factor is a 60-kilodalton protein. These findings provide evidence that DNA damage in mammalian cells induces a factor that can alter the replication of a viral DNA.

  12. [Cu(phen)2](2+) acts as electrochemical indicator and anchor to immobilize probe DNA in electrochemical DNA biosensor.

    PubMed

    Yang, Linlin; Li, Xiaoyu; Li, Xi; Yan, Songling; Ren, Yinna; Wang, Mengmeng; Liu, Peng; Dong, Yulin; Zhang, Chaocan

    2016-01-01

    We demonstrate a novel protocol for sensitive in situ label-free electrochemical detection of DNA hybridization based on copper complex ([Cu(phen)2](2+), where phen = 1,10-phenanthroline) and graphene (GR) modified glassy carbon electrode. Here, [Cu(phen)2](2+) acted advantageously as both the electrochemical indicator and the anchor for probe DNA immobilization via intercalative interactions between the partial double helix structure of probe DNA and the vertical aromatic groups of phen. GR provided large density of docking site for probe DNA immobilization and increased the electrical conductivity ability of the electrode. The modification procedure was monitored by electrochemical impedance spectroscopy (EIS). Square-wave voltammetry (SWV) was used to explore the hybridization events. Under the optimal conditions, the designed electrochemical DNA biosensor could effectively distinguish different mismatch degrees of complementary DNA from one-base mismatch to noncomplementary, indicating that the biosensor had high selectivity. It also exhibited a reasonable linear relationship. The oxidation peak currents of [Cu(phen)2](2+) were linear with the logarithm of the concentrations of complementary target DNA ranging from 1 × 10(-12) to 1 × 10(-6) M with a detection limit of 1.99 × 10(-13) M (signal/noise = 3). Moreover, the stability of the electrochemical DNA biosensor was also studied.

  13. DNA Identification Act of 1993. House of Representatives, One Hundred Third Congress, First Session

    SciTech Connect

    Not Available

    1993-01-01

    Report 103-45 on the DNA Identification Act of 1993 is intended to amend H.R. 829 (to amend title I of the Omnibus Crime Control and Safe Streets Act of 1968 to authorize funds received by States and units of the local government to be expended to improve the quality and availability of DNA records; to authorize the establishment of a DNA identification index; and for other purposes). The report states that the Committee on the Judiciary recommends Bill H.R. 829 pass with the amendment contained in this report. This report explains the amendment, the committee vote on H.R. 829, background behind H.R. 829, quality control concerns, proficincy testing, the significance of DNA matches, and databanking and privacy. Also included is a section-by-section analysis of H.R. 829 and committee oversight findings.

  14. 77 FR 61008 - Request for Comments Under the Paperwork Reduction Act, Section 3506

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-05

    ... Carr, Acting Director, Office of Clinical Research and Bioethics Policy, Office of Science Policy, NIH... 28, 2012. Sarah Carr, Acting Director, Office of Clinical Research and Bioethics Policy, Office...

  15. Does Instruction Alter the Naturalistic Pattern of Pragmatic Development? A Case of Request Speech Act

    ERIC Educational Resources Information Center

    Taguchi, Naoko; Naganuma, Naeko; Budding, Carlos

    2015-01-01

    This study examined the effects of explicit instruction on the development of pragmatic competence in L2 English. The study is based on Taguchi's (2012) study conducted in an English-medium university in Japan, which revealed patterns of change in Japanese EFL students' production of requests in high- and low-imposition situations. Students showed…

  16. 76 FR 19177 - Paperwork Reduction Act of 1995, as amended; Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ... Agency Clearance Officer no later than June 6, 2011. SUPPLEMENTARY INFORMATION: Type of Request: Regular Submission. Title of Information Collection: EnergyRight Program. Frequency of Use: On occasion. Type of... Authority. ACTION: Notice. SUMMARY: The proposed information collection described below will be submitted...

  17. 76 FR 39150 - Paperwork Reduction Act of 1995, as Amended; Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-05

    .... SUPPLEMENTARY INFORMATION: Type of Request: Regular Submission. Title of Information Collection: EnergyRight... Authority. ACTION: Notice. SUMMARY: The proposed information collection described below will be submitted to... on this proposed collection as provided by 5 CFR Section 1320.8(d)(1). FOR FURTHER...

  18. 46 CFR 503.31 - Records available upon written request under the Freedom of Information Act.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... conjunction with the official having responsibility for the subject matter area and the General Counsel, and... provide the record in any form or format requested by the person if the record is readily reproducible by the Secretary in that form or format. (d) Certain fees may be assessed for processing of...

  19. 46 CFR 503.31 - Records available upon written request under the Freedom of Information Act.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... conjunction with the official having responsibility for the subject matter area and the General Counsel, and... provide the record in any form or format requested by the person if the record is readily reproducible by the Secretary in that form or format. (d) Certain fees may be assessed for processing of...

  20. 12 CFR 1204.3 - How do I make a Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... name, current address, and date and place of birth. In order to help identify and locate the records... forms of identification with photographs, or one form of identification with a photograph and a properly authenticated birth certificate. If you make your request by mail, your signature either must be notarized...

  1. DIRECT-ACTING, DNA-DAMAGING AS (III)-METHYLATED SPECIES: IMPLICATIONS FOR A CARCINOGENIC MECHANISM OF ACTION OF ARSENICALS

    EPA Science Inventory

    Direct-acting, DNA-damaging As (III)-methylated species: implications for a carcinogenic . mechanism of action of arsenicals

    Inorganic arsenic (iAs, arsenite and arsenate) has been thought to act as a carcinogen without reacting directly with DNA; neither iAs nor the As(...

  2. DNA evidence in rape cases and the Debbie Smith Act: forensic practice and criminal justice implications.

    PubMed

    Telsavaara, Terhi V T; Arrigo, Bruce A

    2006-10-01

    The Debbie Smith or "Justice for All" Act was passed on November 1, 2004. The act addresses the problem of collecting and analyzing DNA evidence from backlogged rape kits sitting in crime laboratories around the country. Presently, no empirical data exist by which to assess the soundness of the legislation. However, the act clearly affects discrete operations within the forensic and criminal justice systems. This article explores the relative merits of the Debbie Smith law, highlighting changes in Sexual Assault Nurse Examiner (SANE) programs, law enforcement, court administration, correctional treatment, and juvenile justice practices. Concerns linked to the likely impact of the "Justice for All" Act raise significant questions about its overall programmatic utility and treatment efficacy.

  3. Re: Request Under the Data Quality Act and EPA's Information Quality Guidelines

    EPA Pesticide Factsheets

    In light of recent statements from EPA in the rulemaking for the Mercury and Air Toxics Standards (MATS Rule), Environmental Integrity Project (EIP) and Chesapeake Climate Action Network (CCAN) submit this Request for Correction to ask EPA to resolve the conflict between the statements from the MATS rulemaking and EPA's earlier position on the accuracy of monitoring of sulfur dioxide (SO2) under the Acid Rain program.

  4. 78 FR 15705 - Proposed Information Collection; Comment Request; American Fisheries Act (AFA): Permits

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-12

    ... Fisheries Act (AFA): Permits AGENCY: National Oceanic and Atmospheric Administration (NOAA), Commerce... currently approved information collection. In response to the American Fisheries Act (AFA), NMFS developed a management program for Bering Sea and Aleutian Islands Management Area (BSAI) pollock to include a set of...

  5. 78 FR 48462 - Comment Request for Information Collection: American Recovery and Reinvestment Act (ARRA), High...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... Reinvestment Act (ARRA), High Growth and Emerging Industries (HGEI) Grants, Extension With no Revisions AGENCY... for a program of competitive grants for worker training and placement in high growth and emerging... Review: Extension with no revisions. Title: American Recovery and Reinvestment Act (ARRA), High...

  6. Clofarabine Acts as Radiosensitizer In Vitro and In Vivo by Interfering With DNA Damage Response

    SciTech Connect

    Cariveau, Mickael J.; Stackhouse, Murray; Cui Xiaoli; Tiwari, Kamal; Waud, William; Secrist, John A.; Xu Bo

    2008-01-01

    Purpose: Combination treatment with radiotherapy and chemotherapy has emerged as the dominant form of cancer adjuvant regimens in recent years. Clofarabine, a newly approved drug for pediatric leukemia, is a second-generation purine nucleoside analogue that can block DNA synthesis and inhibit DNA repair. Therefore, we hypothesized that clofarabine could work synergistically with radiotherapy to increase the tumor cell response. Methods and Materials: The effects of clofarabine on radiosensitivity have been established in several tumor cell lines in vitro and in vivo using colony-forming assays and tumor xenografts. The effect of clofarabine on the DNA damage response was also studied in vitro by measuring {gamma}-H2AX focus formation. Results: Clonogenic survival was significantly reduced in irradiated cells treated with clofarabine, demonstrating the strong radiosensitizing effect of clofarabine. Furthermore, clofarabine displayed a radiosensitizing effect that was greater than gemcitabine or 5-fluorouracil. We also found that low doses of clofarabine can prolong the presence of radiation-induced {gamma}-H2AX nuclear focus formation, and high doses of clofarabine can induce DNA double-strand breaks, suggesting that clofarabine can interfere with DNA damage response pathways. In addition, clofarabine-induced radiosensitization was also established in vivo using a colorectal cancer model, DLD-1, in athymic nude mice. When combined with fractionated radiotherapy, a moderate dose of clofarabine led to a significant increase in tumor growth inhibition. Conclusion: Clofarabine acts as a powerful radiosensitizer both in vitro and in vivo by interfering with the DNA damage response.

  7. 75 FR 5346 - Comment Request for Information Collection for Workforce Investment Act National Emergency Grant...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-02

    ... and financial resources) is minimized, collection instruments are clearly understood, and the impact... Globalization Adjustment Assistance Act (TGAAA) of 2009; and 20 CFR 671.140. Applications are accepted on...

  8. Privacy Impact Assessment for the Request and Appeal Files for the Freedom of Information Act

    EPA Pesticide Factsheets

    This system collects contact information from Freedom of Information Act (FOIA) requestors. Learn how this data is collected, how it will be used, access to the data, the purpose of data collection, and record retention policies for this data.

  9. Viroid RNA redirects host DNA ligase 1 to act as an RNA ligase.

    PubMed

    Nohales, María-Ángeles; Flores, Ricardo; Daròs, José-Antonio

    2012-08-21

    Viroids are a unique class of noncoding RNAs: composed of only a circular, single-stranded molecule of 246-401 nt, they manage to replicate, move, circumvent host defenses, and frequently induce disease in higher plants. Viroids replicate through an RNA-to-RNA rolling-circle mechanism consisting of transcription of oligomeric viroid RNA intermediates, cleavage to unit-length strands, and circularization. Though the host RNA polymerase II (redirected to accept RNA templates) mediates RNA synthesis and a type-III RNase presumably cleavage of Potato spindle tuber viroid (PSTVd) and closely related members of the family Pospiviroidae, the host enzyme catalyzing the final circularization step, has remained elusive. In this study we propose that PSTVd subverts host DNA ligase 1, converting it to an RNA ligase, for the final step. To support this hypothesis, we show that the tomato (Solanum lycopersicum L.) DNA ligase 1 specifically and efficiently catalyzes circularization of the genuine PSTVd monomeric linear replication intermediate opened at position G95-G96 and containing 5'-phosphomonoester and 3'-hydroxyl terminal groups. Moreover, we also show a decreased PSTVd accumulation and a reduced ratio of monomeric circular to total monomeric PSTVd forms in Nicotiana benthamiana Domin plants in which the endogenous DNA ligase 1 was silenced. Thus, in a remarkable example of parasitic strategy, viroids reprogram for their replication the template and substrate specificity of a DNA-dependent RNA polymerase and a DNA ligase to act as RNA-dependent RNA polymerase and RNA ligase, respectively.

  10. Cis-acting signals modulate the efficiency of programmed DNA elimination in Paramecium tetraurelia

    PubMed Central

    Ferro, Diana; Lepennetier, Gildas; Catania, Francesco

    2015-01-01

    In Paramecium, the regeneration of a functional somatic genome at each sexual event relies on the elimination of thousands of germline DNA sequences, known as Internal Eliminated Sequences (IESs), from the zygotic nuclear DNA. Here, we provide evidence that IESs’ length and sub-terminal bases jointly modulate IES excision by affecting DNA conformation in P. tetraurelia. Our study reveals an excess of complementary base pairing between IESs’ sub-terminal and contiguous sites, suggesting that IESs may form DNA loops prior to cleavage. The degree of complementary base pairing between IESs’ sub-terminal sites (termed Cin-score) is positively associated with IES length and is shaped by natural selection. Moreover, it escalates abruptly when IES length exceeds 45 nucleotides (nt), indicating that only sufficiently large IESs may form loops. Finally, we find that IESs smaller than 46 nt are favored targets of the cellular surveillance systems, presumably because of their relatively inefficient excision. Our findings extend the repertoire of cis-acting determinants for IES recognition/excision and provide unprecedented insights into the distinct selective pressures that operate on IESs and somatic DNA regions. This information potentially moves current models of IES evolution and of mechanisms of IES recognition/excision forward. PMID:26304543

  11. 15 CFR Appendix B to Part 4 - Officials Authorized To Deny Requests for Records Under the Freedom of Information Act, and...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... of Energy, Infrastructure and Machinery Director, Office of Electronic Commerce Director, Office of... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Officials Authorized To Deny Requests... or Amendment Under the Privacy Act B Appendix B to Part 4 Commerce and Foreign Trade Office of...

  12. 10 CFR 905.23 - What are the opportunities for using the Freedom of Information Act to request plan and report data?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false What are the opportunities for using the Freedom of Information Act to request plan and report data? 905.23 Section 905.23 Energy DEPARTMENT OF ENERGY ENERGY PLANNING AND MANAGEMENT PROGRAM Integrated Resource Planning § 905.23 What are the opportunities for...

  13. Assessment of ADHD Documentation from Candidates Requesting Americans with Disabilities Act (ADA) Accommodations for the National Board of Osteopathic Medical Examiners COMLEX Exam

    ERIC Educational Resources Information Center

    Joy, Javed A.; Julius, Rose J.; Akter, Rashida; Baron, David A.

    2010-01-01

    Purpose: Every year increasing numbers of candidates request special accommodations for high-stakes medical licensing examinations, due to ADHD, on the basis of the Americans with Disabilities Act (ADA). This poses significant challenges for both the applicant and the medical boards and has significant financial, legal, and ethical implications.…

  14. 75 FR 49502 - Medical Device User Fee Act; Public Meeting; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-13

    ... before FDA begins negotiations with the regulated industry on medical device user fee program... 738A(b)(2) of the FD&C Act (21 U.S.C. 379j- 1(b)(2)) requires that, before FDA begins negotiations...

  15. 77 FR 4564 - Request for Information Regarding the Reinsurance Program Under the Affordable Care Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-30

    ... responses to this RFI are not offers, and cannot be accepted by the Government to form a binding contract or... contract for services required to fulfill the statutory and regulatory requirements of the reinsurance..., 2011 (76 FR 41930). The Affordable Care Act instructs each State to establish or contract with...

  16. Workforce Investment Act: Better Guidance Needed To Address Concerns over New Requirements. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    Nilsen, Sigurd R.

    In an effort to assess what progress states and localities are making in implementation of the Workforce Investment Act (WIA) of 1998, a study identified three issues of particular concern: (1) mandatory partners' participation in the one-stops; (2) job seekers' ability to receive enhanced choices for training; and (3) private-sector participation…

  17. 77 FR 59224 - Comment Request for Information Collection for the Workforce Investment Act (WIA) Management...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    ... Investment Act (WIA) Management Information and Reporting System; Extension With Revisions AGENCY: Employment... comments concerning the collection of data about the WIA Management Information and Reporting System (OMB... revisions to the WIA Management Information and Reporting System. The first are changes to the...

  18. 77 FR 59899 - Proposed Information Collection; Comment Request; Coastal Zone Management Act Walter B. Jones and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-01

    ... Zone Management Act Walter B. Jones and NOAA Excellence Awards AGENCY: National Oceanic and Atmospheric Administration (NOAA), Commerce. ACTION: Notice. SUMMARY: The Department of Commerce, as part of its continuing... Nedelka, (301) 713-3155 ext. 127 or Patmarie.Nedelka@noaa.gov . SUPPLEMENTARY INFORMATION: I....

  19. Replication of chromosomal and episomal DNA in X-ray-damaged human cells: A cis- or trans-acting mechanism

    SciTech Connect

    Cleaver, J.E.; Rose, R.; Mitchell, D.L. )

    1990-12-01

    Episomal plasmids and viruses in mammalian cells present small targets for X-ray-induced DNA damage. At doses up to 100 Gy, DNA strand breaks or endonuclease III-sensitive sites were not discernible in 10.3-kb Epstein-Barr virus-based plasmid DNA or in 4.9-kb defective simian virus 40 DNA. DNA replication in these small molecules, however, was inhibited strongly by X-ray doses of greater than or equal to 20 Gy, decreasing to only 20 to 40% of control values. Inhibition was relieved slightly by growth in caffeine but was increased by growth in 3-aminobenzamide. Inhibition of DNA replication in episomal DNA molecules that are too small to sustain significant damage directly to their DNA may be due to either (a) a trans-acting diffusible factor that transfers the consequences of DNA breakage to episomes and to other replicating molecules, (b) a cis-acting mechanism in which episomes are structurally linked to genomic chromatin, and replication of both episomal and chromosomal replicons is under common control, or (c) radiation damage on other cellular structures unrelated to DNA. The resolution of these cellular mechanisms may shed light on the X-ray-resistant replication in ataxia-telangiectasia and may suggest strategies for molecular characterization of potential trans- or cis-acting factors.

  20. 76 FR 4747 - Proposed Collection; Comment Request; Bank Secrecy Act Unified Currency Transaction Report...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ...FinCEN is continuing the design of a new Bank Secrecy Act (BSA) database (the Database) and invites comment on the list of proposed data fields within the Database that will be required to support unified Currency Transaction Report (CTR) filings by financial institutions required to file such reports under the BSA. This notice does not propose any new regulatory requirements or changes to the......

  1. DOE FY 2010 Budget Request and Recovery Act Funding for Energy Research, Development, Demonstration, and Deployment: Analysis and Recommendations

    SciTech Connect

    Anadon, Laura Diaz; Gallagher, Kelly Sims; Bunn, Matthew

    2009-06-01

    The combination of the FY 2010 budget request for the Department of Energy (DOE) and the portion of the American Recovery and Reinvestment Act of 2009 (ARRA) funds likely to be available in 2010 would (assuming that they would be split evenly between FY 2010 and FY 2011) result in a doubling in funding available for energy research, development, and deployment (ERD and D) from $3.6 billion in FY 2009 to $7.2 billion in FY 2010. Without the stimulus funds, DOE ERD and D investments in FY 2010 would decrease very slightly when compared to FY 2009. Excluding the $7.5 billion for the Advanced Technology Vehicles Manufacturing Loans in FY 2009, the FY 2010 budget request for deployment represents a 33 percent decrease from the FY 2009 levels from $520 million to $350 million. This decrease is largely due to the large amounts of funds appropriated in ARRA for DOE deployment programs, or $23.6 billion, which are three times greater than those appropriated in the FY 2009 budget. These very substantial funding amounts, coupled with the broad range of institutional innovations the administration is putting in place and movement toward putting a price on carbon emissions, will help accelerate innovation for a broad range of energy technologies. DOE's Advanced Research Projects Agency-Energy (ARPA-E) and the Energy Innovation Hubs are important initiatives that could contribute to two weak points of the government's energy innovation effort, namely funding high-risk projects in transformational technologies and in companies that have not traditionally worked with the government and strengthening the integration of basic and applied research in priority areas. Increasing the funding for different types of energy storage research, providing some support for exploring opportunities in coal-to-liquids with carbon capture and storage (CCS) and coal-and-biomass-to-liquids with CCS, and reducing funding for fission RD and D are other actions that Congress could take in the short

  2. 12 CFR 1204.5 - What if I am dissatisfied with the FHFA response to my Privacy Act request?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... a court of any adverse determination or denial of a request, you first must appeal it under this... available to you. (2)(i) If your appeal concerns an amendment or correction of a record and the appeal determination grants your request for an amendment or correction, the response will describe any amendment...

  3. 75 FR 27672 - Request for Comment on Implementation of the Family Smoking Prevention and Tobacco Control Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ... of cigarettes and smokeless tobacco. The agency is taking this action in response to a request for an... tobacco products are necessary to protect children and adolescents from the harms caused by tobacco use... adolescents from the harms caused by tobacco use. The agency has received a request for a 90-day extension...

  4. 75 FR 60065 - Notice of Request for Extension of Approval of an Information Collection; Lacey Act Declaration...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ...; ] DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service Notice of Request for Extension of...: Animal and Plant Health Inspection Service, USDA. ACTION: Extension of approval of an information... announces the Animal and Plant Health Inspection Service's intention to request an extension of approval...

  5. A novel cis-acting element required for DNA damage-inducible expression of yeast DIN7

    SciTech Connect

    Yoshitani, Ayako; Yoshida, Minoru; Ling Feng

    2008-01-04

    Din7 is a DNA damage-inducible mitochondrial nuclease that modulates the stability of mitochondrial DNA (mtDNA) in Saccharomyces cerevisiae. How DIN7 gene expression is regulated, however, has remained largely unclear. Using promoter sequence alignment, we found a highly conserved 19-bp sequence in the promoter regions of DIN7 and NTG1, which encodes an oxidative stress-inducible base-excision-repair enzyme. Deletion of the 19-bp sequence markedly reduced the hydroxyurea (HU)-enhanced DIN7 promoter activity. In addition, nuclear fractions prepared from HU-treated cells were used in in vitro band shift assays to reveal the presence of currently unidentified trans-acting factor(s) that preferentially bound to the 19-bp region. These results suggest that the 19-bp sequence is a novel cis-acting element that is required for the regulation of DIN7 expression in response to HU-induced DNA damage.

  6. 75 FR 19335 - Premium Review Process; Request for Comments Regarding Section 2794 of the Public Health Service Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... Comments Regarding Section 2794 of the Public Health Service Act AGENCY: Office of the Secretary, HHS... to the Public Health Service Act (the PHS Act). Section 2794 of the PHS Act requires the Secretary to... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH...

  7. Two high-mobility group box domains act together to underwind and kink DNA

    SciTech Connect

    Sánchez-Giraldo, R.; Acosta-Reyes, F. J.; Malarkey, C. S.; Saperas, N.; Churchill, M. E. A.; Campos, J. L.

    2015-06-30

    The crystal structure of HMGB1 box A bound to an unmodified AT-rich DNA fragment is reported at a resolution of 2 Å. A new mode of DNA recognition for HMG box proteins is found in which two box A domains bind in an unusual configuration generating a highly kinked DNA structure. High-mobility group protein 1 (HMGB1) is an essential and ubiquitous DNA architectural factor that influences a myriad of cellular processes. HMGB1 contains two DNA-binding domains, box A and box B, which have little sequence specificity but have remarkable abilities to underwind and bend DNA. Although HMGB1 box A is thought to be responsible for the majority of HMGB1–DNA interactions with pre-bent or kinked DNA, little is known about how it recognizes unmodified DNA. Here, the crystal structure of HMGB1 box A bound to an AT-rich DNA fragment is reported at a resolution of 2 Å. Two box A domains of HMGB1 collaborate in an unusual configuration in which the Phe37 residues of both domains stack together and intercalate the same CG base pair, generating highly kinked DNA. This represents a novel mode of DNA recognition for HMGB proteins and reveals a mechanism by which structure-specific HMG boxes kink linear DNA.

  8. DNA ligase IV and artemis act cooperatively to suppress homologous recombination in human cells: implications for DNA double-strand break repair.

    PubMed

    Kurosawa, Aya; Saito, Shinta; So, Sairei; Hashimoto, Mitsumasa; Iwabuchi, Kuniyoshi; Watabe, Haruka; Adachi, Noritaka

    2013-01-01

    Nonhomologous end-joining (NHEJ) and homologous recombination (HR) are two major pathways for repairing DNA double-strand breaks (DSBs); however, their respective roles in human somatic cells remain to be elucidated. Here we show using a series of human gene-knockout cell lines that NHEJ repairs nearly all of the topoisomerase II- and low-dose radiation-induced DNA damage, while it negatively affects survival of cells harbouring replication-associated DSBs. Intriguingly, we find that loss of DNA ligase IV, a critical NHEJ ligase, and Artemis, an NHEJ factor with endonuclease activity, independently contribute to increased resistance to replication-associated DSBs. We also show that loss of Artemis alleviates hypersensitivity of DNA ligase IV-null cells to low-dose radiation- and topoisomerase II-induced DSBs. Finally, we demonstrate that Artemis-null human cells display increased gene-targeting efficiencies, particularly in the absence of DNA ligase IV. Collectively, these data suggest that DNA ligase IV and Artemis act cooperatively to promote NHEJ, thereby suppressing HR. Our results point to the possibility that HR can only operate on accidental DSBs when NHEJ is missing or abortive, and Artemis may be involved in pathway switching from incomplete NHEJ to HR.

  9. Single-stranded DNA fragments of insect-specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control.

    PubMed

    Oberemok, Volodymyr V; Skorokhod, Oleksii A

    2014-07-01

    This paper focuses on the DNA insecticides as a novel preparation against gypsy moth (Lymantria dispar) based on DNA fragments of the anti-apoptotic gene of its nuclear polyhedrosis virus. It was found that the external application of a solution with two single-stranded DNA fragments from BIR and RING domains of LdMNPV (L.dispar multicapsid nuclear polyhedrosis virus) IAP-3 (inhibitor of apoptosis) gene induces a significantly higher mortality of gypsy moth caterpillars in comparison with the application of the control solutions. This effect does not depend on the infection of caterpillars with LdMNPV. The results also show that DNA insecticides based on LdMNPV IAP-3 gene fragments can be selective in action, and at least are not harmful to tobacco hornworm (Manduca sexta) and black cutworm (Agrotis ipsilon). Part of the gypsy moth genome cloned with the fragments of BIR and RING domains of LdMNPV IAP-3 gene as primers, has an overlap with the corresponding part of the LdMNPV IAP-3 gene and L.dispar IAP-1 mRNA for an inhibitor of apoptosis protein with the high cover by query, allows assuming that we cloned a part of gypsy moth anti-apoptosis gene. This finding gives the grounding that proposed here DNA insecticides might act through the blocking of the mechanisms involved in post transcriptional expression of insect anti-apoptosis genes. The results show the insecticidal potential of the viral genome fragments that can be used to create safe and relatively fast-acting DNA insecticides to control the quantity of gypsy moth populations, important task for forestry and agriculture.

  10. Public Health Service Act, Rural Physician Training Grant Program, definition of "underserved rural community." Interim final rule with request for comment.

    PubMed

    2010-05-26

    This interim final rule (IFR) with request for comment is meant to comply with the statutory directive to issue a regulation defining "underserved rural community" for purposes of the Rural Physician Training Grant Program in section 749B of the Public Health Service Act, as amended by the Patient Protection and Affordable Care Act of 2010. This IFR is technical in nature. It will not change grant or funding eligibility for any other grant program currently available through the Office of Rural Health Policy (ORHP) or HRSA. For purposes of the Rural Physician Training Grant Program only, HRSA has combined existing definitions of "underserved" and "rural" by using the definition of rural utilized by the ORHP Rural Health Grant programs and the definition of "underserved" established by HRSA's Office of Shortage Designation (OSD) in the Bureau of Health Professions (BHPr).

  11. 75 FR 15458 - Request for Small Reclamation Projects Act Loan To Construct Narrows Dam in Sanpete County, UT

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... record. Under the NEPA process, written and oral comments, received by the due date, are given the same... public review period commences with the publication of this notice. Written comments on the SDEIS are due...: Send written comments on the SDEIS and requests for copies to Mr. Peter Crookston, PRO-774, Bureau...

  12. 17 CFR Appendix A to Part 146 - Fees for Copies of Records Requested Under the Privacy Act of 1974

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... by means of an overnight/express service at the fee of $10.00 per unit mailed. (5) The Commission may, upon application by the individual, furnish any records without charge or at a reduced rate, if it determines that such wavier or reduction of fee is in the public interest. b. Requests for copies...

  13. Maternal, Infant and Early Childhood Home Visiting Program in the Affordable Care Act: Supplemental Instruction Request-February 2011

    ERIC Educational Resources Information Center

    Schmit, Stephanie

    2011-01-01

    The latest Evidence-Based Home Visiting Supplemental Information Request (SIR) has recently been released by the Department of Health and Human Services (HHS) with collaboration from the Health Resources and Services Administration (HRSA) and the Administration for Children and Families (ACF) as outlined in the first Funding Opportunity…

  14. 76 FR 30835 - Requests for Modification or Revocation of Toxic Substances Control Act Section 5 Significant New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... (202) 566-0280. Docket visitors are required to show photographic identification, pass through a metal... substitute for paper. 2. Use and acceptance of electronic signatures, when practicable. EPA's Cross-Media... manner set forth in 40 CFR 720.40(a)(2)(i), (ii), or (iii). Paper requests must be submitted either via...

  15. 76 FR 52596 - Request for Comment Concerning Interpretations of the Magnuson-Moss Warranty Act; Rule Governing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... Settlement Procedures; and Guides for the Advertising of Warranties and Guarantees AGENCY: Federal Trade... Advertising of Warranties and Guarantees (``Guides''). The Commission requests public comment on the overall... into their lemon laws. E. 16 CFR Part 239: Guides for the Advertising of Warranties and Guarantees...

  16. 12 CFR 978.4 - Form of request.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REQUESTS FOR INFORMATION § 978.4 Form of request. A request by a Bank to a financial regulatory agency for... and the financial regulatory agency. The request shall reference section 22 of the Act (12 U.S.C....

  17. 78 FR 26374 - An Evaluation of the Prescription Drug User Fee Act Workload Adjuster; Request for Comments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-06

    ... HUMAN SERVICES Food and Drug Administration An Evaluation of the Prescription Drug User Fee Act Workload... on an assessment of the Prescription Drug User Fee Act (PDUFA) Workload Adjuster conducted by an... identified with the docket number found in brackets in the heading of this document. FOR FURTHER...

  18. 76 FR 37057 - Notice of Request for Extension of Approval of an Information Collection; Virus-Serum-Toxin Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    ... Collection; Virus-Serum-Toxin Act and Regulations AGENCY: Animal and Plant Health Inspection Service, USDA... Virus-Serum-Toxin Act and regulations. DATES: We will consider all comments that we receive on or before... (202) 690-2817 before coming. FOR FURTHER INFORMATION CONTACT: For information on the...

  19. Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination

    PubMed Central

    Fredsøe, Jacob; Nielsen, Ida; Pedersen, Jakob Madsen; Bentsen, Iben Bach; Lisby, Michael; Bjergbaek, Lotte; Andersen, Anni H

    2015-01-01

    Faithful DNA replication with correct termination is essential for genome stability and transmission of genetic information. Here we have investigated the potential roles of Topoisomerase II (Top2) and the RecQ helicase Sgs1 during late stages of replication. We find that cells lacking Top2 and Sgs1 (or Top3) display two different characteristics during late S/G2 phase, checkpoint activation and accumulation of asymmetric X-structures, which are both independent of homologous recombination. Our data demonstrate that checkpoint activation is caused by a DNA structure formed at the strongest rDNA replication fork barrier (RFB) during replication termination, and consistently, checkpoint activation is dependent on the RFB binding protein, Fob1. In contrast, asymmetric X-structures are formed independent of Fob1 at less strong rDNA replication fork barriers. However, both checkpoint activation and formation of asymmetric X-structures are sensitive to conditions, which facilitate fork merging and progression of replication forks through replication fork barriers. Our data are consistent with a redundant role of Top2 and Sgs1 together with Top3 (Sgs1-Top3) in replication fork merging at rDNA barriers. At RFB either Top2 or Sgs1-Top3 is essential to prevent formation of a checkpoint activating DNA structure during termination, but at less strong rDNA barriers absence of the enzymes merely delays replication fork merging, causing an accumulation of asymmetric termination structures, which are solved over time. PMID:26630413

  20. Dysregulated human Tyrosyl-DNA phosphodiesterase I acts as cellular toxin

    PubMed Central

    Cuya, Selma M.; Comeaux, Evan Q.; Wanzeck, Keith; Yoon, Karina J.; van Waardenburg, Robert C.A.M.

    2016-01-01

    Tyrosyl-DNA phosphodiesterase I (TDP1) hydrolyzes the drug-stabilized 3’phospho-tyrosyl bond formed between DNA topoisomerase I (TOPO1) and DNA. TDP1-mediated hydrolysis uses a nucleophilic histidine (Hisnuc) and a general acid/base histidine (Hisgab). A Tdp1Hisgab to Arg mutant identified in patients with the autosomal recessive neurodegenerative disease SCAN1 causes stabilization of the TDP1-DNA intermediate. Based on our previously reported Hisgab-substitutions inducing yeast toxicity (Gajewski et al. J. Mol. Biol. 415, 741-758, 2012), we propose that converting TDP1 into a cellular poison by stabilizing the covalent enzyme-DNA intermediate is a novel therapeutic strategy for cancer treatment. Here, we analyzed the toxic effects of two TDP1 catalytic mutants in HEK293 cells. Expression of human Tdp1HisnucAla and Tdp1HisgabAsn mutants results in stabilization of the covalent TDP1-DNA intermediate and induces cytotoxicity. Moreover, these mutants display reduced in vitro catalytic activity compared to wild type. Co-treatment of Tdp1mutant with topotecan shows more than additive cytotoxicity. Overall, these results support the hypothesis that stabilization of the TDP1-DNA covalent intermediate is a potential anti-cancer therapeutic strategy. PMID:27893431

  1. Bacillus subtilis glutamine synthetase regulates its own synthesis by acting as a chaperone to stabilize GlnR-DNA complexes.

    PubMed

    Fisher, Susan H; Wray, Lewis V

    2008-01-22

    The Bacillus subtilis GlnR repressor controls gene expression in response to nitrogen availability. Because all GlnR-regulated genes are expressed constitutively in mutants lacking glutamine synthetase (GS), GS is required for repression by GlnR. Feedback-inhibited GS (FBI-GS) was shown to activate GlnR DNA binding with an in vitro electophoretic mobility shift assay (EMSA). The activation of GlnR DNA binding by GS in these experiments depended on the feedback inhibitor glutamine and did not occur with mutant GS proteins defective in regulating GlnR activity in vivo. Although stable GS-GlnR-DNA ternary complexes were not observed in the EMSA experiments, cross-linking experiments showed that a protein-protein interaction occurs between GlnR and FBI-GS. This interaction was reduced in the absence of the feedback inhibitor glutamine and with mutant GS proteins. Because FBI-GS significantly reduced the dissociation rate of the GlnR-DNA complexes, the stability of these complexes is enhanced by FBI-GS. These results argue that FBI-GS acts as a chaperone that activates GlnR DNA binding through a transient protein-protein interaction that stabilizes GlnR-DNA complexes. GS was shown to control the activity of the B. subtilis nitrogen transcription factor TnrA by forming a stable complex between FBI-GS and TnrA that inhibits TnrA DNA binding. Thus, B. subtilis GS is an enzyme with dual catalytic and regulatory functions that uses distinct mechanisms to control the activity of two different transcription factors.

  2. cis-Acting components of human papillomavirus (HPV) DNA replication: linker substitution analysis of the HPV type 11 origin.

    PubMed Central

    Russell, J; Botchan, M R

    1995-01-01

    Papillomavirus DNA replication requires the viral trans-acting factors E1 and E2 in addition to the host cell's general replication machinery. The origins of DNA replication in bovine and human papillomavirus genomes have been localized to a specific part of the upstream regulatory region (URR) which includes recognition sites for E1 and E2 proteins. To fine map cis-acting elements influencing human papillomavirus type 11 (HPV-11) DNA replication and to determine the relative contributions of such sites, we engineered consecutive linker substitution mutations across a region of 158 bp in the HPV-11 origin and tested mutant origins for replication function in a cell-based transient replication assay. Our results both confirm and extend the findings of others. E2 binding sites are the major cis components of HPV-11 DNA replication, and there is evidence for synergy between these sites. Differential capacity of the three E2 binding sites within the origin to affect replication may be attributed, at least in part, to context. At least one E2 binding site is essential for replication. The imperfect AT-rich palindrome of the E1 helicase binding site is not essential since replication occurs even in the absence of this sequence. However, replication is enhanced by the presence of the palindromic sequence in the HPV-11 origin. Sequence components adjacent to the E1 and E2 binding sites, comprising AT-rich and purine-rich elements and the consensus TATA box sequence, probably contribute to the overall efficiency of replication, though they are nonessential. None of the other cis elements of the HPV-11 origin region analyzed seems to influence replication significantly in the system described. The HPV-11 origin of DNA replication therefore differs from those of the other papovaviruses, simian virus 40 and polyomavirus, inasmuch as an intact helicase binding site and adjacent AT-rich components, while influential, are not absolutely essential. PMID:7815528

  3. Antibacterial effects of Brazilian and Bulgarian propolis and synergistic effects with antibiotics acting on the bacterial DNA and folic acid.

    PubMed

    Orsi, R O; Fernandes, A; Bankova, V; Sforcin, J M

    2012-01-01

    Propolis is a honeybee product that has been used since ancient times because of its therapeutic effects. It can be used in the development of alternative therapies for the treatment of many diseases, and because propolis shows antibacterial action, this work was carried out in order to investigate a possible synergism between propolis and antibiotics acting on DNA (ciprofloxacin and norfloxacin) and on the metabolism (cotrimoxazole) against Salmonella typhi. Propolis samples collected in Brazil and Bulgaria were compared in these assays, and the synergism was investigated by using ½ and ¼ of the minimal inhibitory concentration for propolis and antibiotics, evaluating the number of viable cells according to the incubation time. Brazilian and Bulgarian propolis showed antibacterial activity, but no synergistic effects with the three tested antibiotics were seen. Previous works by our laboratory have revealed that propolis has synergistic effects with antibiotics, acting on the bacterial wall and ribosome, but it does not seem to interact with antibiotics acting on DNA or folic acid, and only a bacteriostatic action was seen in these assay conditions.

  4. 78 FR 32390 - Food and Drug Administration Safety and Innovation Act (FDASIA): Request for Comments on the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-30

    ... HUMAN SERVICES Office of the Secretary Food and Drug Administration Safety and Innovation Act (FDASIA... Information Technology AGENCY: Office of the National Coordinator for Health Information Technology...: The Food and Drug Administration (FDA), Office of the National Coordinator for Health...

  5. 75 FR 13241 - Request for Comment on Implementation of the Family Smoking Prevention and Tobacco Control Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... Implementation of the Family Smoking Prevention and Tobacco Control Act AGENCY: Food and Drug Administration, HHS... protect children and adolescents as required by the Family Smoking Prevention and Tobacco Control...

  6. What does it take? California county funding requests for recovery-oriented full service partnerships under the Mental Health Services Act.

    PubMed

    Felton, Mistique C; Cashin, Cheryl E; Brown, Timothy T

    2010-10-01

    The need to move mental health systems toward more recovery-oriented treatment modes is well established. Progress has been made to define needed changes but evidence is lacking about the resources required to implement them. The Mental Health Services Act (MHSA) in California was designed to implement more recovery-oriented treatment modes. We use data from county funding requests and annual updates to examine how counties budgeted for recovery-oriented programs targeted to different age groups under MHSA. Findings indicate that initial per-client budgeting for Full Services Partnerships under MHSA was maintained in future cycles and counties budgeted less per client for children. With this analysis, we begin to benchmark resource allocation for programs that are intended to be recovery-oriented, which should be evaluated against appropriate outcome measures in the future to determine the degree of recovery-orientation.

  7. FAN1 acts with FANCI-FANCD2 to promote DNA interstrand cross-link repair.

    PubMed

    Liu, Ting; Ghosal, Gargi; Yuan, Jingsong; Chen, Junjie; Huang, Jun

    2010-08-06

    Fanconi anemia (FA) is caused by mutations in 13 Fanc genes and renders cells hypersensitive to DNA interstrand cross-linking (ICL) agents. A central event in the FA pathway is mono-ubiquitylation of the FANCI-FANCD2 (ID) protein complex. Here, we characterize a previously unrecognized nuclease, Fanconi anemia-associated nuclease 1 (FAN1), that promotes ICL repair in a manner strictly dependent on its ability to accumulate at or near sites of DNA damage and that relies on mono-ubiquitylation of the ID complex. Thus, the mono-ubiquitylated ID complex recruits the downstream repair protein FAN1 and facilitates the repair of DNA interstrand cross-links.

  8. Statewide Educational Accountability Under the No Child Left Behind Act: A Report on 2006 Amendments to State Plans. A Summary of State Requests in 2005-06 for Amendments to Their Educational Accountability Systems Under NCLB

    ERIC Educational Resources Information Center

    Forte, Ellen; Erpenbach, William J.

    2006-01-01

    This report summarizes requests submitted by states in 2005-06 to amend their educational accountability plans under the No Child Left Behind Act (NCLB) of 2001 as well as the related decisions of the U.S. Department of Education (ED). This fourth installment of the Council of Chief State School Officers' (CCSSO's) annual review of changes to…

  9. 76 FR 12360 - Request for Information on Implementation of the James Zadroga 9/11 Health and Compensation Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND... the James Zadroga 9/ 11 Health and Compensation Act of 2010 (Pub. L. 111-347) AGENCY: National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention...

  10. 76 FR 30717 - Notice Pursuant to Executive Order 12600 of Receipt of Freedom of Information Act (FOIA) Requests...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-26

    ... Compensation as required under section 2 of the Federal Funding Accountability and Transparency Act of 2006... Exempt status Public comments 1) CAGE CODE Not exempt under the FOIA. 2) CCR EXTRACT CODE Not exempt... under the FOIA. 10) ST ADD (2) Not exempt under the FOIA. 11) CITY Not exempt under the FOIA. 12)...

  11. Workforce Investment Act: States' Spending Is on Track, But Better Guidance Would Improve Financial Reporting. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    General Accounting Office, Washington, DC.

    The U.S. Congress asked the Government Accounting Office (GAO) to determine the following: (1) to what extent states were spending their Workforce Investment Act (WIA) funds and whether the Department of Labor's (Labor's) data accurately reflected available funds; (2) what Labor did to assess how states were managing their WIA spending; and (3)…

  12. 16 CFR 4.11 - Disclosure requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... RULES § 4.11 Disclosure requests. (a) Freedom of Information Act requests—(1) Initial requests—(i) Form and contents; time of receipt. (A) A request under the provisions of the Freedom of Information Act, 5... follows: Freedom of Information Act Request, Office of the General Counsel, Federal Trade Commission,...

  13. 32 CFR 300.7 - FOIA request processing procedures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) FREEDOM OF INFORMATION ACT PROGRAM DEFENSE LOGISTICS AGENCY FREEDOM OF INFORMATION ACT PROGRAM... request is perfected. When a DLA FOIA Requester Service Center receives a request for records that clearly... Service Center has a telephone line to inform the requester of the status of their request (see Appendix...

  14. Scabin, a Novel DNA-acting ADP-ribosyltransferase from Streptomyces scabies.

    PubMed

    Lyons, Bronwyn; Ravulapalli, Ravikiran; Lanoue, Jason; Lugo, Miguel R; Dutta, Debajyoti; Carlin, Stephanie; Merrill, A Rod

    2016-05-20

    A bioinformatics strategy was used to identify Scabin, a novel DNA-targeting enzyme from the plant pathogen 87.22 strain of Streptomyces scabies Scabin shares nearly 40% sequence identity with the Pierisin family of mono-ADP-ribosyltransferase toxins. Scabin was purified to homogeneity as a 22-kDa single-domain enzyme and was shown to possess high NAD(+)-glycohydrolase (Km (NAD) = 68 ± 3 μm; kcat = 94 ± 2 min(-1)) activity with an RSQXE motif; it was also shown to target deoxyguanosine and showed sigmoidal enzyme kinetics (K0.5(deoxyguanosine) = 302 ± 12 μm; kcat = 14 min(-1)). Mass spectrometry analysis revealed that Scabin labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA. Several small molecule inhibitors were identified, and the most potent compounds were found to inhibit the enzyme activity with Ki values ranging from 3 to 24 μm PJ34, a well known inhibitor of poly-ADP-ribosyltransferases, was shown to be the most potent inhibitor of Scabin. Scabin was crystallized, representing the first structure of a DNA-targeting mono-ADP-ribosyltransferase enzyme; the structures of the apo-form (1.45 Å) and with two inhibitors (P6-E, 1.4 Å; PJ34, 1.6 Å) were solved. These x-ray structures are also the first high resolution structures of the Pierisin subgroup of the mono-ADP-ribosyltransferase toxin family. A model of Scabin with its DNA substrate is also proposed.

  15. 29 CFR 70.19 - Requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... by mail, delivery service/courier, facsimile or e-mail. (b) To whom to direct requests. A request... Information Act Request.” If the request is made by e-mail, it must be sent to foiarequest@dol.gov. Requests submitted to any other e-mail address will not be accepted as a request made under this part. (1)...

  16. Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism.

    PubMed Central

    Singer, J D; Manning, B M; Formosa, T

    1996-01-01

    We have developed a genetic screen of the yeast Saccharomyces cerevisiae to identify genes that act to coordinate DNA replication so that each part of the genome is copied exactly once per cell cycle. A mutant was recovered in this screen that accumulates aberrantly high DNA contents but does not complete a second round of synthesis. The mutation principally responsible for this phenotype is in the DOA4 gene, which encodes a ubiquitin hydrolase, one of several yeast genes that encode enzymes that can remove the signalling polypeptide ubiquitin hydrolase, one of several yeast genes that encode enzymes that can remove the signaling polypeptide ubiquitin from its covalently linked conjugated forms. DOA4 is nonessential, and deleting this gene causes uncoordinated replication. Overreplication does not occur in cells with limiting amounts of Cdc7 protein kinase, suggesting that entry into S phase is required for this phenotype. The DNA formed in doa4 mutants is not highly unusual in the sense that mitotic recombination rates are normal, implying that a high level of repair is not induced. The temperature sensitivity of doa4 mutations is partially suppressed by extra copies of the polyubiquitin gene UB14, but overreplication still occurs in the presence of this suppressor. Mutations in DOA4 cause loss of the free ubiquitin pool in cells under heat stress conditions, and extra copies of UB14 restore this pool without restoring coordination of replication. We conclude that a ubiquitin-mediated signaling event directly involving the ubiquitin hydrolase encoded by DOA4 is needed in S. cerevisiae to prevent uncoordinated DNA replication. PMID:8657109

  17. 45 CFR 2106.2 - Requests for access.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... WITH 5 U.S.C. 552a, THE PRIVACY ACT OF 1974 § 2106.2 Requests for access. (a) Requirement for written.... The request should state that the request is pursuant to the Privacy Act of 1974. In the absence of... system of records, the request will be presumed to be made under the Privacy Act of 1974. The...

  18. 45 CFR 2106.2 - Requests for access.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... WITH 5 U.S.C. 552a, THE PRIVACY ACT OF 1974 § 2106.2 Requests for access. (a) Requirement for written.... The request should state that the request is pursuant to the Privacy Act of 1974. In the absence of... system of records, the request will be presumed to be made under the Privacy Act of 1974. The...

  19. 45 CFR 2106.2 - Requests for access.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... WITH 5 U.S.C. 552a, THE PRIVACY ACT OF 1974 § 2106.2 Requests for access. (a) Requirement for written.... The request should state that the request is pursuant to the Privacy Act of 1974. In the absence of... system of records, the request will be presumed to be made under the Privacy Act of 1974. The...

  20. [Fourth evaluation of the law on the review of termination of life on request and assisted suicide (Euthanasia Act)].

    PubMed

    van der Heide, A; Onwuteaka-Philipsen, B D; van Delden, J J M; Gevers, J K M; van der Maas, P J; van der Wal, G

    2005-09-24

    This fall, an extensive study will start to evaluate the Dutch Euthanasia Act. This law was enacted in 2002. According to this law, physicians must report cases of euthanasia and physician-assisted suicide. The cases are then judged by regional euthanasia review committees consisting of a lawyer, a physician and an ethicist. Only if they conclude that the case does not meet the requirements for prudent practice, it will be sent to the public prosecutor. The study will be focused on the practice of medical end-of-life decision-making, the functioning and effects of the Euthanasia Act, and opinions of physicians about the scope of the law and the demarcation between different end-of-life decisions. The study will comprise 4 sub-studies: a judicial evaluation, a death certificate study, a survey among physicians and a panel study among physicians, nurses, members of euthanasia review committees, lawyers and ethicists. This study is the fourth in a row of nationwide studies into end-of-life practices that have been performed since 1990. The previous studies contributed to the public debate about medical care at the end of life and to the development of policy in this field. It is expected that this study, by providing up-to-date information on and insight into end-of-life care in the Netherlands, will do the same.

  1. A saturation screen for cis-acting regulatory DNA in the Hox genes of Ciona intestinalis

    SciTech Connect

    Keys, David N.; Lee, Byung-in; Di Gregorio, Anna; Harafuji, Naoe; Detter, Chris; Wang, Mei; Kahsai, Orsalem; Ahn, Sylvia; Arellano, Andre; Zhang, Quin; Trong, Stephan; Doyle, Sharon A.; Satoh, Noriyuki; Satou, Yutaka; Saiga, Hidetoshi; Christian, Allen; Rokhsar, Dan; Hawkins, Trevor L.; Levine, Mike; Richardson, Paul

    2005-01-05

    A screen for the systematic identification of cis-regulatory elements within large (>100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis. Randomly generated DNA fragments from bacterial artificial chromosomes containing two clusters of Hox genes were inserted into a vector upstream of a minimal promoter and lacZ reporter gene. A total of 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulatory activities were monitored in larvae. In sum, 21 separable cis-regulatory elements were found. These include eight Hox linked domains that drive expression in nested anterior-posterior domains of ectodermally derived tissues. In addition to vertebrate-like CNS regulation, the discovery of cis-regulatory domains that drive epidermal transcription suggests that C. intestinalis has arthropod-like Hox patterning in the epidermis.

  2. Chromosomal position effects reveal different cis-acting requirements for rDNA transcription and sex chromosome pairing in Drosophila melanogaster.

    PubMed Central

    Briscoe, A; Tomkiel, J E

    2000-01-01

    In Drosophila melanogaster, the rDNA loci function in ribosome biogenesis and nucleolar formation and also as sex chromosome pairing sites in male meiosis. These activities are not dependent on the heterochromatic location of the rDNA, because euchromatic transgenes are competent to form nucleoli and restore pairing to rDNA-deficient X chromosomes. These transgene studies, however, do not address requirements for the function of the endogenous rDNA loci within the heterochromatin. Here we describe two chromosome rearrangements that disrupt rDNA functions. Both rearrangements are translocations that cause an extreme bobbed visible phenotype and XY nondisjunction and meiotic drive in males. However, neither rearrangement interacts with a specific Y chromosome, Ymal(+), that induces male sterility in combination with rDNA deletions. Molecular studies show that the translocations are not associated with gross rearrangements of the rDNA repeat arrays. Rather, suppression of the bobbed phenotypes by Y heterochromatin suggests that decreased rDNA function is caused by a chromosomal position effect. While both translocations affect rDNA transcription, only one disrupts meiotic XY pairing, indicating that there are different cis-acting requirements for rDNA transcription and rDNA-mediated meiotic pairing. PMID:10880481

  3. Immunomodulatory drugs act as inhibitors of DNA methyltransferases and induce PU.1 up-regulation in myeloma cells.

    PubMed

    Endo, Shinya; Amano, Masayuki; Nishimura, Nao; Ueno, Niina; Ueno, Shikiko; Yuki, Hiromichi; Fujiwara, Shiho; Wada, Naoko; Hirata, Shinya; Hata, Hiroyuki; Mitsuya, Hiroaki; Okuno, Yutaka

    2016-01-08

    Immunomodulatory drugs (IMiDs) such as thalidomide, lenalidomide, and pomalidomide are efficacious in the treatment of multiple myeloma and significantly prolong their survival. However, the mechanisms of such effects of IMiDs have not been fully elucidated. Recently, cereblon has been identified as a target binding protein of thalidomide. Lenalidomide-resistant myeloma cell lines often lose the expression of cereblon, suggesting that IMiDs act as an anti-myeloma agent through interacting with cereblon. Cereblon binds to damaged DNA-binding protein and functions as a ubiquitin ligase, inducing degradation of IKZF1 and IKZF3 that are essential transcription factors for B and T cell development. Degradation of both IKZF1 and IKZF3 reportedly suppresses myeloma cell growth. Here, we found that IMiDs act as inhibitors of DNA methyltransferases (DMNTs). We previously reported that PU.1, which is an ETS family transcription factor and essential for myeloid and lymphoid development, functions as a tumor suppressor in myeloma cells. PU.1 induces growth arrest and apoptosis of myeloma cell lines. In this study, we found that low-dose lenalidomide and pomalidomide up-regulate PU.1 expression through inducing demethylation of the PU.1 promoter. In addition, IMiDs inhibited DNMT1, DNMT3a, and DNMT3b activities in vitro. Furthermore, lenalidomide and pomalidomide decreased the methylation status of the whole genome in myeloma cells. Collectively, IMiDs exert demethylation activity through inhibiting DNMT1, 3a, and 3b, and up-regulating PU.1 expression, which may be one of the mechanisms of the anti-myeloma activity of IMiDs.

  4. Methyl-CpG binding domain protein acts to regulate the repair of cyclobutane pyrimidine dimers on rice DNA

    PubMed Central

    Fang, Changxun; Chen, Weisi; Li, Chengxun; Jian, Xin; Li, Yingzhe; Lin, Hongmei; Lin, Wenxiong

    2016-01-01

    UVB radiation causes cyclobutane pyrimidine dimers (CPDs) to form on the DNA of living organisms. This study found that overexpression of the silicon absorbance gene Lsi1 reduced the accumulation of CPDs in rice, which profited from the reactivation by photolyase. The transcript abundance of deoxyribodipyrimidine photolyase (Os10g0167600) was generally correlated with the silicon content of the rice, and the up-regulation of Os10g0167600 was found to be highest in the UVB-treated Lsi1-overexpressed (Lsi1-OX) rice. A trans-acting factor, methyl-CpG binding domain protein (OsMeCP), was found to interact with the cis-element of Os10g0167600. The nucleic location of OsMeCP effectively enabled the transcriptional regulation. Compared with the WT, the level of OsMeCP was lower in the Lsi1-OX rice but higher in the Lsi1-RNAi line. Rice cultured in a high silicate-concentration solution also exhibited less OsMeCP abundance. Overexpression of OsMeCP led to lower Os10g0167600 transcript levels and a higher CPD content than in the WT, but the reverse was true in the OsMeCP-RNAi line. These findings indicate that OsMeCP acts as a negative regulator of silicon, and can mediate the repression of the transcription from Os10g0167600, which inhibits the photoreactivation of the photolyase involved in the repair of CPDs. PMID:27694845

  5. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  6. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  7. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  8. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  9. 7 CFR 1220.600 - Act.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Procedures To Request a Referendum Definitions § 1220.600 Act. Act means the...

  10. 7 CFR 1.6 - Aggregating requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Aggregating requests. 1.6 Section 1.6 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Official Records § 1.6 Aggregating requests. When an agency reasonably believes that a requester, or a group of requesters acting in...

  11. 7 CFR 1.6 - Aggregating requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Aggregating requests. 1.6 Section 1.6 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Official Records § 1.6 Aggregating requests. When an agency reasonably believes that a requester, or a group of requesters acting in...

  12. 45 CFR 1171.7 - Timing of responses to requests.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... FREEDOM OF INFORMATION ACT § 1171.7 Timing of responses to requests. (a) In general. The NEH customarily... requester must submit a statement, certified to be true and correct to the requester's best knowledge...

  13. 14 CFR 1212.300 - Requesting amendment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Requesting amendment. 1212.300 Section 1212.300 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Amendments to Privacy Act Records § 1212.300 Requesting amendment. Individuals may request that NASA...

  14. 16 CFR 4.11 - Disclosure requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... RULES § 4.11 Disclosure requests. (a) Freedom of Information Act—(1) Initial requests—(i) Form and contents; time of receipt. (A) A request under the provisions of the Freedom of Information Act, 5 U.S.C... following means: by mail to the following address: Freedom of Information Act Request, Office of the...

  15. p53 in the mitochondria, as a trans-acting protein, provides error-correction activities during the incorporation of non-canonical dUTP into DNA.

    PubMed

    Bonda, Elad; Rahav, Galia; Kaya, Angelina; Bakhanashvili, Mary

    2016-11-08

    Mutations in mitochondrial DNA is an outcome of errors produced by DNA polymerase γ during replication and failure of the repair mechanism. Misincorporation of non-canonical dUTP leads to mutagenesis or apoptosis, and may contribute to the cytotoxic effects of 5'-fluorouracil chemotherapy. Tumor suppressor p53 protein in the mitochondria displays physical and functional interactions with mitochondrial DNA and polymerase γ, and by its intrinsic 3'→5' exonuclease activity can diminish the polymerization errors. Here we demonstrate the impact of p53 on incorporation of uracil into DNA examined with mitochondrial fractions, as the source of polymerase γ. p53 in mitochondria facilitates DNA damage repair functions resulting from uracil-DNA misincorporation. Our biochemical studies revealed that the procession of U:A and mismatched U:G lesions enhances in the presence of recombinant or endogenous cytoplasmic p53. p53 in mitochondria can function as an exonuclease/proofreader for polymerase γ by either decreasing the incorporation of non-canonical dUTP into DNA or by promoting the excision of incorporated nucleotide from nascent DNA, thus expanding the spectrum of DNA damage sites exploited for proofreading as a trans-acting protein. The data suggest that p53 may contribute to defense of the cells from consequences of dUTP misincorporation in both normal and tumor cells.

  16. p53 in the mitochondria, as a trans-acting protein, provides error-correction activities during the incorporation of non-canonical dUTP into DNA

    PubMed Central

    Bonda, Elad; Rahav, Galia; Kaya, Angelina; Bakhanashvili, Mary

    2016-01-01

    Mutations in mitochondrial DNA is an outcome of errors produced by DNA polymerase γ during replication and failure of the repair mechanism. Misincorporation of non-canonical dUTP leads to mutagenesis or apoptosis, and may contribute to the cytotoxic effects of 5′-fluorouracil chemotherapy. Tumor suppressor p53 protein in the mitochondria displays physical and functional interactions with mitochondrial DNA and polymerase γ, and by its intrinsic 3′→5′ exonuclease activity can diminish the polymerization errors. Here we demonstrate the impact of p53 on incorporation of uracil into DNA examined with mitochondrial fractions, as the source of polymerase γ. p53 in mitochondria facilitates DNA damage repair functions resulting from uracil–DNA misincorporation. Our biochemical studies revealed that the procession of U:A and mismatched U:G lesions enhances in the presence of recombinant or endogenous cytoplasmic p53. p53 in mitochondria can function as an exonuclease/proofreader for polymerase γ by either decreasing the incorporation of non-canonical dUTP into DNA or by promoting the excision of incorporated nucleotide from nascent DNA, thus expanding the spectrum of DNA damage sites exploited for proofreading as a trans-acting protein. The data suggest that p53 may contribute to defense of the cells from consequences of dUTP misincorporation in both normal and tumor cells. PMID:27689337

  17. 10 CFR 9.23 - Requests for records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...)(3) to the Freedom of Information Act and Privacy Act Officer by an appropriate method listed in § 9... until the date it is actually received by the Freedom of Information Act and Privacy Act Officer. (1) A... Freedom of Information Act and Privacy Act Officer receives the request. A request does not cover...

  18. An amphipathic trans-acting phosphorothioate DNA element delivers uncharged PNA and PMO nucleic acid sequences in mammalian cells

    PubMed Central

    Jain, Harsh V.; Beaucage, Serge L.

    2016-01-01

    An innovative approach to the delivery of uncharged peptide nucleic acids (PNA) and phosphorodiamidate morpholino (PMO) oligomers in mammalian cells is described and consists of extending the sequence of those oligomers with a short PNA-polyA or PMO-polyA tail. Recognition of the polyA-tailed PNA or PMO oligomers by an amphipathic trans-acting polythymidylic thiophosphate triester element (dTtaPS) results in efficient internalization of those oligomers in several cell lines. Our findings indicate that cellular uptake of the oligomers occurs through an energy-dependent mechanism and macropinocytosis appears to be the predo-minant endocytic pathway used for internalization. The functionality of the internalized oligomers is demonstrated by alternate splicing of the pre-mRNA encoding luciferase in HeLa pLuc 705 cells. Amphipathic phosphorothioate DNA elements may represent a unique class of cellular transporters for robust delivery of uncharged nucleic acid sequences in live mammalian cells. PMID:27516815

  19. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  20. 45 CFR 1602.8 - Requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... DISCLOSURE OF INFORMATION UNDER THE FREEDOM OF INFORMATION ACT § 1602.8 Requests for records. (a) Except for... clearly marked Freedom of Information Act Request. All such requests shall be addressed to the Corporation... submit a statement demonstrating a compelling need that is certified by the requester to be true...

  1. 22 CFR 505.3 - Procedures for requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Procedures for requests. 505.3 Section 505.3 Foreign Relations BROADCASTING BOARD OF GOVERNORS PRIVACY ACT REGULATION § 505.3 Procedures for requests... treated as a Privacy Act request. The remaining portions of such a request will be processed as a...

  2. 45 CFR 1602.8 - Requests for records.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... DISCLOSURE OF INFORMATION UNDER THE FREEDOM OF INFORMATION ACT § 1602.8 Requests for records. (a) Except for... clearly marked Freedom of Information Act Request. All such requests shall be addressed to the Corporation... submit a statement demonstrating a compelling need that is certified by the requester to be true...

  3. 22 CFR 505.3 - Procedures for requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 2 2013-04-01 2009-04-01 true Procedures for requests. 505.3 Section 505.3 Foreign Relations BROADCASTING BOARD OF GOVERNORS PRIVACY ACT REGULATION § 505.3 Procedures for requests... treated as a Privacy Act request. The remaining portions of such a request will be processed as a...

  4. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  5. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  6. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  7. 36 CFR 810.2 - Procedure for requesting information.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... FREEDOM OF INFORMATION ACT REGULATIONS § 810.2 Procedure for requesting information. (a) Requests for... should be clearly marked “FREEDOM OF INFORMATION REQUEST” in order to ensure timely processing. Requests... of the Council staff as being a request pursuant to the Freedom of Information Act. (b)...

  8. 10 CFR 1705.05 - Processing of requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Processing of requests. 1705.05 Section 1705.05 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.05 Processing of requests. (a) Requests in writing. The Privacy Act Officer will acknowledge receipt of the request within five working days of...

  9. 5 CFR 9301.14 - Requests for access.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... RECORDS AND INFORMATION Privacy Act § 9301.14 Requests for access. (a) Requirement for written requests... shall be addressed to the Privacy Officer at the address cited above, and shall name the system of... request is pursuant to the Privacy Act of 1974. In the absence of such a statement, if the request is...

  10. 5 CFR 9301.14 - Requests for access.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... RECORDS AND INFORMATION Privacy Act § 9301.14 Requests for access. (a) Requirement for written requests... shall be addressed to the Privacy Officer at the address cited above, and shall name the system of... request is pursuant to the Privacy Act of 1974. In the absence of such a statement, if the request is...

  11. 77 FR 34415 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ... COMMISSION Proposed Collection; Comment Request Upon Written Request, Copies Available From: U.S. Securities... Exchange Commission (``Commission'') is soliciting comments on the existing collection of information... et seq.) (``Exchange Act''). The Commission plans to submit this existing collection of...

  12. 12 CFR 404.18 - Requests for correction of records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 4 2011-01-01 2011-01-01 false Requests for correction of records. 404.18... Records Under the Privacy Act of 1974 § 404.18 Requests for correction of records. (a) Form. Requests for correction must be made in writing and must be signed by the requester. Requests should be addressed to...

  13. 12 CFR 404.18 - Requests for correction of records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Requests for correction of records. 404.18... Records Under the Privacy Act of 1974 § 404.18 Requests for correction of records. (a) Form. Requests for correction must be made in writing and must be signed by the requester. Requests should be addressed to...

  14. 12 CFR 1102.306 - Procedures for requesting records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Information Act Request” at his or her e-mail address listed on the ASC's World Wide Web site. (2) The request... corrected request, which will be treated as a new request. (c) Processing requests—(1) Receipt of requests... sound administrative process, the ASC processes requests in the order they are received. However, in...

  15. PKBalpha/Akt1 acts downstream of DNA-PK in the DNA double-strand break response and promotes survival.

    PubMed

    Bozulic, Lana; Surucu, Banu; Hynx, Debby; Hemmings, Brian A

    2008-04-25

    Protein kinase B (PKB/Akt) is a well-established regulator of several essential cellular processes. Here, we report a route by which activated PKB promotes survival in response to DNA insults in vivo. PKB activation following DNA damage requires 3-phosphoinositide-dependent kinase 1 (PDK1) and DNA-dependent protein kinase (DNA-PK). Active PKB localizes in the nucleus of gamma-irradiated cells adjacent to DNA double-strand breaks, where it colocalizes and interacts with DNA-PK. Levels of active PKB inversely correlate with DNA damage-induced apoptosis. A significant portion of p53- and DNA damage-regulated genes are misregulated in cells lacking PKBalpha. PKBalpha knockout mice show impaired DNA damage-dependent induction of p21 and increased tissue apoptosis after single-dose whole-body irradiation. Our findings place PKB downstream of DNA-PK in the DNA damage response signaling cascade, where it provides a prosurvival signal, in particular by affecting transcriptional p21 regulation. Furthermore, this function is apparently restricted to the PKBalpha isoform.

  16. 76 FR 78662 - Proposed Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    ...; Comment Request Proposed Projects: Title: Plan for Foster Care and Adoption Assistance--Title IV-E. OMB No... Act (the Act) for each public child welfare agency requesting Federal funding for foster...

  17. 77 FR 38637 - Proposed Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-28

    ...; Comment Request Proposed Projects Title: Plan for Foster Care and Adoption Assistance--Title IV-E. OMB No... Act (the Act) for each public child welfare agency requesting Federal funding for foster...

  18. 22 CFR 171.35 - Denials of requests; appeals.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... RECORDS TO THE PUBLIC Privacy Act Provisions § 171.35 Denials of requests; appeals. If the Department denies a request for access to Privacy Act records, for amendment of such records, or for an...

  19. 75 FR 32416 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ... Parkway, Aurora, CO 80011-9066. The request should contain the full name, address and the signature of the... Act Requester Service, 16401 Centretech Parkway, Aurora, CO 80011-9066. The request should contain...

  20. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... the request and on the front of the envelope and directed to the Privacy Act Officer. (c) Requests...

  1. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... the request and on the front of the envelope and directed to the Privacy Act Officer. (c) Requests...

  2. DNA.

    ERIC Educational Resources Information Center

    Felsenfeld, Gary

    1985-01-01

    Structural form, bonding scheme, and chromatin structure of and gene-modification experiments with deoxyribonucleic acid (DNA) are described. Indicates that DNA's double helix is variable and also flexible as it interacts with regulatory and other molecules to transfer hereditary messages. (DH)

  3. 43 CFR 2.27 - How will a bureau handle a request for information that is contained in a Privacy Act system of...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... information that is contained in a Privacy Act system of records? (See DOI's Privacy Act regulations (Subpart... system of records? (See DOI's Privacy Act regulations (Subpart G of this part) for additional information... information, usually an original signature (see the appropriate Privacy Act system notice and, subpart G...

  4. 11 CFR 4.7 - Requests for records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Elections FEDERAL ELECTION COMMISSION PUBLIC RECORDS AND THE FREEDOM OF INFORMATION ACT § 4.7 Requests for records. (a) (b)(1) Requests for copies of records pursuant to the Freedom of Information Act shall be... process requests under the Freedom of Information Act that is based on the amount of work and/or...

  5. 43 CFR 2.62 - Requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Requests for access to records. 2.62... OF INFORMATION ACT Privacy Act § 2.62 Requests for access to records. The Privacy Act permits individuals, upon request, to gain access to their records or to any information pertaining to them which...

  6. 32 CFR 1665.2 - Requests for access.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM PRIVACY ACT... description of such system of records. The request should state that the request is pursuant to the Privacy Act of 1974. In the absence of specifying solely the Privacy Act of 1974 and, if the request may...

  7. 40 CFR 1602.2 - Requests for access to records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PRIVACY AND ACCESS TO INDIVIDUAL RECORDS UNDER THE PRIVACY ACT OF 1974 § 1602.2 Requests for access to... should mark both your request letter and the envelope “Privacy Act Request.” (b) Description of records... systems of records also may be found as part of the “Privacy Act Compilation” published by the...

  8. 32 CFR 1665.2 - Requests for access.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM PRIVACY ACT... description of such system of records. The request should state that the request is pursuant to the Privacy Act of 1974. In the absence of specifying solely the Privacy Act of 1974 and, if the request may...

  9. 1 CFR 304.21 - Requests for access to records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... INFORMATION Protection of Privacy and Access to Individual Records Under the Privacy Act of 1974 § 304.21... possible handling of a mail request, you should mark both your request letter and the envelope “Privacy Act... its systems of records. (c) Agreement to pay fees. If you make a Privacy Act request for access...

  10. 77 FR 5481 - Notice of Request for Extension of Approval of an Information Collection; Special Need Requests...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ... Collection; Special Need Requests Under the Plant Protection Act AGENCY: Animal and Plant Health Inspection... INFORMATION CONTACT: For information on special need requests under the Plant Protection Act, contact Ms. Lynn...: Title: Special Need Request Under the Plant Protection Act. OMB Number: 0579-0291. Type of...

  11. 78 FR 56225 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Soil...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-12

    ... AGENCY Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Soil...) for review and approval in accordance with the Paperwork Reduction Act: ``Soil Fumigant Risk... measures necessary for reregistration eligibility for certain soil fumigant chemicals are...

  12. The plasmid replicon of EBV consists of multiple cis-acting elements that facilitate DNA synthesis by the cell and a viral maintenance element.

    PubMed Central

    Aiyar, A; Tyree, C; Sugden, B

    1998-01-01

    Plasmids containing oriP, the plasmid origin of Epstein-Barr virus (EBV), are replicated stably in human cells that express a single viral trans-acting factor, EBNA-1. Unlike plasmids of other viruses, but akin to human chromosomes, oriP plasmids are synthesized once per cell cycle, and are partitioned faithfully to daughter cells during mitosis. Although EBNA-1 binds multiple sites within oriP, its role in DNA synthesis and partitioning has been obscure. EBNA-1 lacks enzymatic activities that are present in the origin-binding proteins of other mammalian viruses, and does not interact with human cellular proteins that provide equivalent enzymatic functions. We demonstrate that plasmids with oriP or its constituent elements are synthesized efficiently in human cells in the absence of EBNA-1. Further, we show that human cells rapidly eliminate or destroy newly synthesized plasmids, and that both EBNA-1 and the family of repeats of oriP are required for oriP plasmids to escape this catastrophic loss. These findings indicate that EBV's plasmid replicon consists of genetic elements with distinct functions, multiple cis-acting elements that facilitate DNA synthesis and viral cis/trans elements that permit retention of replicated DNA in daughter cells. They also explain historical failures to identify mammalian origins of DNA synthesis as autonomously replicating sequences. PMID:9799247

  13. 36 CFR 902.14 - Deletion of nondiscloseable information from requested records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AVENUE DEVELOPMENT CORPORATION FREEDOM OF INFORMATION ACT General Administration § 902.14 Deletion of nondiscloseable information from requested records. Whenever a requested record contains information which...

  14. A T7exonuclease-assisted target recycling amplification with graphene oxide acting as the signal amplifier for fluorescence polarization detection of human immunodeficiency virus (HIV) DNA.

    PubMed

    Wang, Lijun; Tian, Jianniao; Yang, Wen; Zhao, Yanchun; Zhao, Shulin

    2016-03-01

    We report a fluorescence polarization (FP) platform for human immunodeficiency virus (HIV) DNA detection based on T7exonuclease-assisted target recycling amplification with graphene oxide (GO) acting as a FP signal amplifier. In the sensing method, the presence of the target DNA leads to target recycling with the assistance of T7exonuclease, furthermore, the amplification products are absorbed onto the surface of GO, so the all FP values are enhanced by GO. More importantly, this FP sensor exhibits high detection sensitivity; under optimal conditions, the change in FP is linear with the concentration of the target DNA within a concentration range of 50-2000 pmol/L, and the detection limit of this method is as low as 38.6 pmol/L. This FP sensor also exhibits high selectivity, even single-base mismatched DNA can be effectively discriminated from complementary target DNA. Above all, the proposed FP sensor may serve as a general platform for the sensitive assay of disease-related genes.

  15. 22 CFR 1502.4 - Written requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 2 2013-04-01 2009-04-01 true Written requests. 1502.4 Section 1502.4 Foreign Relations AFRICAN DEVELOPMENT FOUNDATION AVAILABILITY OF RECORDS § 1502.4 Written requests. In order to... marked: “Freedom of Information Act Request.” (b) Identify the desired record by name, title, author,...

  16. 22 CFR 1502.4 - Written requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Written requests. 1502.4 Section 1502.4 Foreign Relations AFRICAN DEVELOPMENT FOUNDATION AVAILABILITY OF RECORDS § 1502.4 Written requests. In order to... marked: “Freedom of Information Act Request.” (b) Identify the desired record by name, title, author,...

  17. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  18. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  19. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  20. 28 CFR 47.4 - Written request.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Written request. 47.4 Section 47.4 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) RIGHT TO FINANCIAL PRIVACY ACT § 47.4 Written request. (a) The formal written request shall be in the form of a letter or memorandum to an...

  1. The Crucial Role of Divalent Metal Ions in the DNA-Acting Efficacy and Inhibition of the Transcription of Dimeric Chromomycin A3

    PubMed Central

    Hsu, Chun-Wei; Chuang, Show-Mei; Wu, Wen-Ling; Hou, Ming-Hon

    2012-01-01

    Chromomycin A3 (Chro) is capable of forming a stable dimeric complex via chelation with Ni(II), Fe(II) and Co(II). According to the circular dichroism study, the dimer conformations are significantly different among the Fe(II)-, Co(II)-, and Ni(II)-containing dimeric Chro complexes; however, the dimer conformations were preserved at high temperatures. Furthermore, we conducted a systematic study to determine the effects of these divalent metal ions on the DNA-acting efficacy of dimeric Chro, including its DNA-binding affinity, DNA stabilization capacity, DNA cleavage activity, and the inhibition of transcription both in vitro and within cells. Kinetic analyses using surface plasmon resonance (SPR) showed that NiII(Chro)2 exhibited the highest Ka with a value of 1.26×107 M−1, which is approximately 1.6- and 3.7-fold higher than the Ka values obtained for CoII(Chro)2 and FeII(Chro)2, respectively. The Tm and ΔG values for the DNA duplex increased after the addition of drug complexes in the following order: NiII(Chro)2>CoII(Chro)2>FeII(Chro)2. In the DNA integrity assays, the DNA cleavage rate of CoII(Chro)2 (1.2×10−3 s−1) is higher than those of FeII(Chro)2 and NiII(Chro)2, which were calculated to be 1×10−4 and 3.1×10−4 s−1, respectively. Consistent with the SPR and UV melting results, NiII(Chro)2 possesses the highest inhibitory effect on in vitro transcription and c-myc transcription within cells compared to CoII(Chro)2 and FeII(Chro)2. By comparing the cytotoxicity among CoII(Chro)2, FeII(Chro)2, and NiII(Chro)2 to several cancer cell lines, our studies concluded that NiII(Chro)2 displayed more potential antitumor activities than CoII(Chro)2 and FeII(Chro)2 did due to its higher DNA-acting efficacy. Changes to the divalent metal ions in the dimeric Chro complexes have been correlated with improved anticancer profiles. The availability of new metal derivatives of Chro may introduce new possibilities for exploiting the unique properties of this

  2. DNA

    ERIC Educational Resources Information Center

    Stent, Gunther S.

    1970-01-01

    This history for molecular genetics and its explanation of DNA begins with an analysis of the Golden Jubilee essay papers, 1955. The paper ends stating that the higher nervous system is the one major frontier of biological inquiry which still offers some romance of research. (Author/VW)

  3. New orally active DNA minor groove binding small molecule CT-1 acts against breast cancer by targeting tumor DNA damage leading to p53-dependent apoptosis.

    PubMed

    Saini, Karan Singh; Hamidullah; Ashraf, Raghib; Mandalapu, Dhanaraju; Das, Sharmistha; Siddiqui, Mohd Quadir; Dwivedi, Sonam; Sarkar, Jayanta; Sharma, Vishnu Lal; Konwar, Rituraj

    2017-04-01

    Targeting tumor DNA damage and p53 pathway is a clinically established strategy in the development of cancer chemotherapeutics. Majority of anti-cancer drugs are delivered through parenteral route for reasons like severe toxicity, lack of stability, and poor enteral absorption. Current DNA targeting drugs in clinical like anthracycline suffers from major drawbacks like cardiotoxicity. Here, we report identification of a new orally active small molecule curcumin-triazole conjugate (CT-1) with significant anti-breast cancer activity in vitro and in vivo. CT-1 selectively and significantly inhibits viability of breast cancer cell lines; retards cells cycle progression at S phase and induce mitochondrial-mediated cell apoptosis. CT-1 selectively binds to minor groove of DNA and induces DNA damage leading to increase in p53 along with decrease in its ubiquitination. Inhibition of p53 with pharmacological inhibitor as well as siRNA revealed the necessity of p53 in CT-1-mediated anti-cancer effects in breast cancer cells. Studies using several other intact p53 and deficient p53 cancer cell lines further confirmed necessity of p53 in CT-1-mediated anti-cancer response. Pharmacological inhibition of pan-caspase showed CT-1 induces caspase-dependent cell death in breast cancer cells. Most interestingly, oral administration of CT-1 induces significant inhibition of tumor growth in LA-7 syngeneic orthotropic rat mammary tumor model. CT-1 treated mammary tumor shows enhancement in DNA damage, p53 upregulation, and apoptosis. Collectively, CT-1 exhibits potent anti-cancer effect both in vitro and in vivo and could serve as a safe orally active lead for anti-cancer drug development. © 2016 Wiley Periodicals, Inc.

  4. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction of records. (a) Correction requests. Any person is entitled to request correction of his or...

  5. 4 CFR 200.9 - Requests for correction of records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4 Accounts 1 2010-01-01 2010-01-01 false Requests for correction of records. 200.9 Section 200.9 Accounts RECOVERY ACCOUNTABILITY AND TRANSPARENCY BOARD PRIVACY ACT OF 1974 § 200.9 Requests for correction of records. (a) Correction requests. Any person is entitled to request correction of his or...

  6. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction of records. (a) Correction requests. Any person is entitled to request correction of his or...

  7. 5 CFR 2100.7 - Request for correction or amendment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Request for correction or amendment. 2100... PRIVACY ACT PROCEDURES § 2100.7 Request for correction or amendment. (a) Requests to correct or amend a... amendment (e.g., deletion, correction, amendment), and the reason for the amendment. The request should...

  8. 4 CFR 200.9 - Requests for correction of records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 4 Accounts 1 2011-01-01 2011-01-01 false Requests for correction of records. 200.9 Section 200.9 Accounts RECOVERY ACCOUNTABILITY AND TRANSPARENCY BOARD PRIVACY ACT OF 1974 § 200.9 Requests for correction of records. (a) Correction requests. Any person is entitled to request correction of his or...

  9. 5 CFR 2100.7 - Request for correction or amendment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Request for correction or amendment. 2100... PRIVACY ACT PROCEDURES § 2100.7 Request for correction or amendment. (a) Requests to correct or amend a... amendment (e.g., deletion, correction, amendment), and the reason for the amendment. The request should...

  10. 22 CFR 1304.4 - Requests for records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Requests for records. 1304.4 Section 1304.4 Foreign Relations MILLENNIUM CHALLENGE CORPORATION FREEDOM OF INFORMATION ACT PROCEDURES § 1304.4 Requests for records. (a) Request requirements. Requests for access to, or copies of, MCC records shall be...

  11. 22 CFR 1304.4 - Requests for records.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 2 2013-04-01 2009-04-01 true Requests for records. 1304.4 Section 1304.4 Foreign Relations MILLENNIUM CHALLENGE CORPORATION FREEDOM OF INFORMATION ACT PROCEDURES § 1304.4 Requests for records. (a) Request requirements. Requests for access to, or copies of, MCC records shall be...

  12. 36 CFR 1600.4 - Timing of responses to requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... EXCELLENCE IN NATIONAL ENVIRONMENTAL POLICY FOUNDATION PUBLIC AVAILABILITY OF DOCUMENTS AND RECORDS... request can be expected to be completed. Where the extension is for more than 10 working days, the... requester, or by a group of requesters acting in concert, constitute a single request that would...

  13. 29 CFR 2702.3 - Requests for information.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... receipt. In the event of a tie vote of those Commissioners, the Chief FOIA Officer's initial determination... appropriately examine a voluminous amount of separate and distinct records that are requested in a single... requests by the same requester, or a group of requesters acting in concert, actually constitute a...

  14. 29 CFR 2201.5 - Procedure for requesting records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... itself. Requests for information must describe the particular record requested to the fullest extent... 29 Labor 9 2010-07-01 2010-07-01 false Procedure for requesting records. 2201.5 Section 2201.5... REGULATIONS IMPLEMENTING THE FREEDOM OF INFORMATION ACT § 2201.5 Procedure for requesting records....

  15. 29 CFR 2201.5 - Procedure for requesting records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... itself. Requests for information must describe the particular record requested to the fullest extent... 29 Labor 9 2013-07-01 2013-07-01 false Procedure for requesting records. 2201.5 Section 2201.5... REGULATIONS IMPLEMENTING THE FREEDOM OF INFORMATION ACT § 2201.5 Procedure for requesting records....

  16. 29 CFR 2201.5 - Procedure for requesting records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... itself. Requests for information must describe the particular record requested to the fullest extent... 29 Labor 9 2012-07-01 2012-07-01 false Procedure for requesting records. 2201.5 Section 2201.5... REGULATIONS IMPLEMENTING THE FREEDOM OF INFORMATION ACT § 2201.5 Procedure for requesting records....

  17. 12 CFR 404.14 - Requirements of request for access.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Records Under the Privacy Act of 1974 § 404.14 Requirements of request for access. (a) Form. Requests for... Freedom of Information and Privacy Office at the address in § 404.12(e) and should contain both the return... number in the request in order to facilitate the identification and location of the requested records....

  18. Barrier to auto integration factor becomes dephosphorylated during HSV-1 Infection and Can Act as a host defense by impairing viral DNA replication and gene expression.

    PubMed

    Jamin, Augusta; Thunuguntla, Prasanth; Wicklund, April; Jones, Clinton; Wiebe, Matthew S

    2014-01-01

    BAF (Barrier to Autointegration Factor) is a highly conserved DNA binding protein that senses poxviral DNA in the cytoplasm and tightly binds to the viral genome to interfere with DNA replication and transcription. To counteract BAF, a poxviral-encoded protein kinase phosphorylates BAF, which renders BAF unable to bind DNA and allows efficient viral replication to occur. Herein, we examined how BAF phosphorylation is affected by herpes simplex virus type 1 (HSV-1) infection and tested the ability of BAF to interfere with HSV-1 productive infection. Interestingly, we found that BAF phosphorylation decreases markedly following HSV-1 infection. To determine whether dephosphorylated BAF impacts HSV-1 productive infection, we employed cell lines stably expressing a constitutively unphosphorylated form of BAF (BAF-MAAAQ) and cells overexpressing wild type (wt) BAF for comparison. Although HSV-1 production in cells overexpressing wtBAF was similar to that in cells expressing no additional BAF, viral growth was reduced approximately 80% in the presence of BAF-MAAAQ. Experiments were also performed to determine the mechanism of the antiviral activity of BAF with the following results. BAF-MAAAQ was localized to the nucleus, whereas wtBAF was dispersed throughout cells prior to infection. Following infection, wtBAF becomes dephosphorylated and relocalized to the nucleus. Additionally, BAF was associated with the HSV-1 genome during infection, with BAF-MAAAQ associated to a greater extent than wtBAF. Importantly, unphosphorylated BAF inhibited both viral DNA replication and gene expression. For example, expression of two regulatory proteins, ICP0 and VP16, were substantially reduced in cells expressing BAF-MAAAQ. However, other viral genes were not dramatically affected suggesting that expression of certain viral genes can be differentially regulated by unphosphorylated BAF. Collectively, these results suggest that BAF can act in a phosphorylation-regulated manner to impair

  19. Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.

    PubMed Central

    Blow, J J; Watson, J V

    1987-01-01

    We have used a novel approach to investigate the control of initiation of replication of sperm nuclei in a Xenopus cell-free extract. Nascent DNA was labelled with biotin by supplementing the extract with biotin-11-dUTP, and isolated nuclei were then probed with fluorescein-conjugated streptavidin. Flow cytometry was used to measure the biotin content of individual nuclei and their total DNA content. This showed that incorporation of the biotinylated precursor increases linearly with DNA content. Haploid sperm nuclei replicate fully to reach the diploid DNA content over 2-6 h in the extract. Synthesis stops once the diploid DNA content is reached. Different nuclei enter S phase at different times over greater than 1.5 h, although they share the same cytoplasmic environment. Nuclei reach their maximum rates of synthesis soon after entry into S phase and some replicate fully in less than 0.5 h, resembling the rates of replication observed in the intact egg. These results indicate that initiations are coordinated within each nucleus such that the nucleus is the fundamental unit of replication in the cell-free system. Images Fig. 1. Fig. 2. PMID:3653079

  20. 12 CFR 505.3 - Requests for records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 6 2013-01-01 2012-01-01 true Requests for records. 505.3 Section 505.3 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FREEDOM OF INFORMATION ACT § 505...) whether to grant a request for OTS records. Requests may be mailed to: Freedom of Information Act...

  1. 12 CFR 505.3 - Requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true Requests for records. 505.3 Section 505.3 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FREEDOM OF INFORMATION ACT § 505...) whether to grant a request for OTS records. Requests may be mailed to: Freedom of Information Act...

  2. 18 CFR 401.103 - Request for existing records.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true Request for existing... routine distribution to the public shall be deemed to be a request for records pursuant to the Freedom of Information Act, whether or not the Freedom of Information Act is mentioned in the request, and shall...

  3. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  4. 4 CFR 200.6 - Processing of requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4 Accounts 1 2010-01-01 2010-01-01 false Processing of requests. 200.6 Section 200.6 Accounts RECOVERY ACCOUNTABILITY AND TRANSPARENCY BOARD PRIVACY ACT OF 1974 § 200.6 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain whether the...

  5. 12 CFR 309.5 - Procedures for requesting records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... processing. A requester may contact the FOIA/PA Group to learn whether a particular request has been assigned... a free-lance journalist who can demonstrate a reasonable expectation of having his or her work... Act/Privacy Act Group (“FOIA/PA Group”), Legal Division : (i) By completing the online request...

  6. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  7. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  8. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  9. 10 CFR 1304.106 - Processing of requests.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Processing of requests. 1304.106 Section 1304.106 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.106 Processing of requests. Upon receipt of a request for information, the Privacy Act Officer will ascertain: Whether the records...

  10. 28 CFR 700.11 - Request for access to records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... OR INFORMATION OF THE OFFICE OF INDEPENDENT COUNSEL Protection of Privacy and Access to Individual Records Under the Privacy Act of 1974 § 700.11 Request for access to records. (a) Procedure for making..., DC 20004. Both the envelope and the request itself should be marked: “Privacy Act Request.”...

  11. 28 CFR 700.11 - Request for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OR INFORMATION OF THE OFFICE OF INDEPENDENT COUNSEL Protection of Privacy and Access to Individual Records Under the Privacy Act of 1974 § 700.11 Request for access to records. (a) Procedure for making..., DC 20004. Both the envelope and the request itself should be marked: “Privacy Act Request.”...

  12. Cholera toxin B subunit acts as a potent systemic adjuvant for HIV-1 DNA vaccination intramuscularly in mice.

    PubMed

    Hou, Jue; Liu, Ying; Hsi, Jenny; Wang, Hongzhi; Tao, Ran; Shao, Yiming

    2014-01-01

    Cholera toxin B subunit (CTB) was investigated as a classical mucosal adjuvant that can increase vaccine immunogenicity. In this study, we found out the in vitro efficacy of cholera toxin B subunit (CTB) in activating mice bone marrow-derived dendritic cells (BMDCs) through Toll-like receptor signaling pathways. In vitro RNA and transcriptional level profiling arrays revealed that CTB guides high levels of Th1 and Th2 type cytokines, inflammatory cytokines, and chemokines. Based on the robustness of these profiling results, we examined the induction of HIV Env-specific immunity by CTB co-inoculated with HIV Env DNA vaccine intramuscularly in vivo. CTB enhanced HIV-Env specific cellular immune responses in Env-specific IFN-γ ELISPOT, compared with DNA vaccine alone. Moreover, CTB induced high levels of Env specific humoral response and promoted antibody maturation after the third round of vaccination. This combination immunization strategy induced a Th2-type bias response which is indicative of a high ratio of IgG1/IgG2a. This study reports that CTB as a classical mucosal adjuvant could enhance HIV-1 DNA-based vaccine immunogenicity intramuscularly; therefore, these findings suggest that CTB could serve as an effective candidate adjuvant for DNA vaccination.

  13. Embryonic caffeine exposure acts via A1 adenosine receptors to alter adult cardiac function and DNA methylation in mice.

    PubMed

    Buscariollo, Daniela L; Fang, Xiefan; Greenwood, Victoria; Xue, Huiling; Rivkees, Scott A; Wendler, Christopher C

    2014-01-01

    Evidence indicates that disruption of normal prenatal development influences an individual's risk of developing obesity and cardiovascular disease as an adult. Thus, understanding how in utero exposure to chemical agents leads to increased susceptibility to adult diseases is a critical health related issue. Our aim was to determine whether adenosine A1 receptors (A1ARs) mediate the long-term effects of in utero caffeine exposure on cardiac function and whether these long-term effects are the result of changes in DNA methylation patterns in adult hearts. Pregnant A1AR knockout mice were treated with caffeine (20 mg/kg) or vehicle (0.09% NaCl) i.p. at embryonic day 8.5. This caffeine treatment results in serum levels equivalent to the consumption of 2-4 cups of coffee in humans. After dams gave birth, offspring were examined at 8-10 weeks of age. A1AR+/+ offspring treated in utero with caffeine were 10% heavier than vehicle controls. Using echocardiography, we observed altered cardiac function and morphology in adult mice exposed to caffeine in utero. Caffeine treatment decreased cardiac output by 11% and increased left ventricular wall thickness by 29% during diastole. Using DNA methylation arrays, we identified altered DNA methylation patterns in A1AR+/+ caffeine treated hearts, including 7719 differentially methylated regions (DMRs) within the genome and an overall decrease in DNA methylation of 26%. Analysis of genes associated with DMRs revealed that many are associated with cardiac hypertrophy. These data demonstrate that A1ARs mediate in utero caffeine effects on cardiac function and growth and that caffeine exposure leads to changes in DNA methylation.

  14. 77 FR 61599 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Trade...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-10

    ... information collection request (ICR), ``Trade Secret Claims for Emergency Planning and Community Right-to-Know... AGENCY Information Collection Request Submitted to OMB for Review and Approval; Comment Request; Trade Secret Claims for Emergency Planning and Community Right-to-Know Act (Renewal) AGENCY:...

  15. 77 FR 43822 - Proposed Information Collection Request; Comment Request; Valuing Improved Water Quality in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-26

    ... AGENCY Proposed Information Collection Request; Comment Request; Valuing Improved Water Quality in the... request (ICR), ``Valuing Improved Water Quality in the Chesapeake Bay Using Stated Preference Methods (New... Act (CWA) directs EPA to coordinate Federal and State efforts to improve water quality in...

  16. 76 FR 35442 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-17

    ... HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is publishing the following summary of a proposed information collection request for public comment....

  17. The water-soluble Roussin's red ester acting as a potential photochemical NO-delivery agent: photolysis reactions, DNA cleavage and anticancer activity.

    PubMed

    Chang, Han-Hun; Huang, Hung-Jen; Ho, Yun-Lung; Wen, Yu-Der; Huang, Wei-Ning; Chiou, Show-Jen

    2009-08-28

    The water-soluble Roussin's red ester [(NO)(2)Fe(mu-SCH(2)CH(2)P(O)(CH(2)OH)(2))(2)Fe(NO)(2)] (1), a potential photochemical prodrug of an NO precursor, was synthesized from the reaction of HSCH(2)CH(2)P(O)(CH(2)OH)(2) (F) and [Fe(CO)(2)(NO)(2)]. The IR v(NO) stretching frequencies of complex 1 appear at 1759 (s), 1784 (s) and 1816 (w) cm(-1) in buffer (pH = 7.4). NO was released with a stoichiometry ratio Delta[NO]/Delta[1] = 3.6 +/- 0.2 when complex 1 was exposed to UV in deaerated aqueous phosphate buffer solution. Here light acts as an On/Off switch for NO release. Incubation of pBR322 supercoiled DNA with complex 1, followed by irradiation, produced DNA strand breakage. In contrast to the addition of carboxy-PTIO (NO radical scavenger), DNA strand breakage was not inhibited when the scavengers of hydroxyl radical and singlet oxygen were added. Complex 1 irradiated under a N(2) atmosphere exhibited the same cleavage efficiency as complex 1 irradiated under air. The results show that DNA strand cleavage efficiency depends on the concentration of complex 1, the pH value of the buffer, and the duration of the photolysis of complex 1. The conversion rate from supercoiled (SC form) to nicked circular (NC form) of complex 1 was 2.96 x 10(-2) s(-1). The results of a T4 ligase enzymatic assay reveals the nonhydrolytic DNA breakage mechanism. The NO-release ability of complexes 1, 2, and 3 follows the order 1 > 2 > 3. Upon UV-irradiation, complex 1 exhibits cytotoxicity against B16-F10 mouse melanoma cells.

  18. Group health plans and health insurance issuers relating to coverage of preventive services under the Patient Protection and Affordable Care Act. Interim final rules with request for comments.

    PubMed

    2011-08-03

    This document contains amendments to the interim final regulations implementing the rules for group health plans and health insurance coverage in the group and individual markets under provisions of the Patient Protection and Affordable Care Act regarding preventive health services.

  19. cis-acting DNA regulatory elements, including the retinoic acid response element, are required for tissue specific laminin B1 promoter/lacZ expression in transgenic mice.

    PubMed

    Sharif, K A; Li, C; Gudas, L J

    2001-05-01

    The LAMB1 gene encodes the laminin beta1 subunit of laminin, an extracellular matrix protein. Using several transgenic mouse lines containing various lengths of the LAMB1 promoter driving lacZ reporter gene expression, regions of LAMB1 promoter that contain cis-acting DNA regulatory element(s) have been identified. The 3.9LAMB1betagal transgene is expressed in various tissues during development. LAMB1 transgene expression is observed in a selective set of nephrons of the neonatal and adult kidneys. The cis-acting DNA regulatory elements responsible for LAMB1 transgene expression in ovaries and in juvenile kidneys are present between -'1.4 and -0.7 kb relative to the transcription start site, while those of adult kidneys are located between -2.5 and -1.4 kb. The LAMB1 transgene is also expressed in the epididymis of 1 week old transgenic mice. Mutation of the retinoic acid response element (RARE) in the context of the 3.9LAMB1betagal transgene results in loss of LAMB1 transgene expression in all tissues. Thus, sequences between -2.5 and -0.7 kb plus the RARE are required for appropriate expression of the LAMB1 transgene in mice.

  20. Protective effects of garlic sulfur compounds against DNA damage induced by direct- and indirect-acting genotoxic agents in HepG2 cells.

    PubMed

    Belloir, C; Singh, V; Daurat, C; Siess, M H; Le Bon, A M

    2006-06-01

    The aim of this study was to assess the antigenotoxic activity of several garlic organosulfur compounds (OSC) in the human hepatoma cell line HepG2, using comet assay. The OSC selected were allicin (DADSO), diallyl sulfide (DAS), diallyl disulfide (DADS), S-allyl cysteine (SAC) and allyl mercaptan (AM). To explore their potential mechanisms of action, two approaches were performed: (i) a pre-treatment protocol which allowed study of the possible modulation of drug metabolism enzymes by OSC before treatment of the cells with the genotoxic agent; (ii) a co-treatment protocol by which the ability of OSC to scavenge direct-acting compounds was assessed. Preliminary studies showed that, over the concentration range tested (5-100 microM), the studied OSC neither affected cell viability nor induced DNA damage by themselves. In the pre-treatment protocol, aflatoxin B1 genotoxicity was significantly reduced by all the OSC tested except AM. DADS was the most efficient OSC in reducing benzo(a)pyrene genotoxicity. SAC and AM significantly decreased DNA breaks in HepG2 cells treated with dimethylnitrosamine. Additionally, all the OSC studied were shown to decrease the genotoxicity of the direct-acting compounds, hydrogen peroxide and methyl methanesulfonate. This study demonstrated that garlic OSC displayed antigenotoxic activity in human metabolically competent cells.

  1. 75 FR 20829 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-21

    ... Submission for OMB Review; Comment Request AGENCY: Department of Education. SUMMARY: The Acting Director... substantially interfere with any agency's ability to perform its statutory obligations. The Acting Director... comment. Dated: April 16, 2010. James Hyler, Acting Director, Information Collection Clearance...

  2. Cajanol inhibits the growth of Escherichia coli and Staphylococcus aureus by acting on membrane and DNA damage.

    PubMed

    Liu, Xiao-lei; Zhang, Xin-jian; Fu, Yu-jie; Zu, Yuan-gang; Wu, Nan; Liang, Lu; Efferth, Thomas

    2011-01-01

    In the present study, the mechanism of antibacterial activity of cajanol extracted from the roots of Cajanus cajan (L.) Millsp. towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was investigated. The antibacterial activity of cajanol was evaluated towards six bacterial strains (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa) by the broth microdilution method. It showed strong antibacterial activity towards all bacteria tested with minimal inhibition concentration (MIC) values ranging from 98.90 µM to 197.8 µM. Cajanol-induced death rates in the most sensitive strains ( E.COLI, 96.55 % and S. AUREUS, 97.25 %) were analyzed by flow cytometry. Furthermore, the activity of cajanol on the membranes of E. coli and S. aureus was investigated by using lecithin, phosphate groups, and fluorescence microscopy. Cajanol-induced DNA damage was observed by agarose gel electrophoresis. In summary, cajanol inhibited E. coli only by DNA damage, whereas S. aureus was inhibited by affecting both, the lecithin and phosphate groups on the cellular membrane and DNA. The present study shows that cajanol possesses antibacterial activity in vitro towards both gram-negative and gram-positive bacteria and therefore may be a promising candidate as an antibacterial agent for the therapy of microbial infections.

  3. 47 CFR 0.461 - Requests for inspection of materials not routinely available for public inspection.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... be an agency record subject to the requirements of the Freedom of Information Act when maintained by... the requested form or format. (b)(1) Requests shall be captioned “Freedom of Information Act Request... the request is enclosed in an envelope, the envelope shall be marked, “Freedom of Information...

  4. 41 CFR 51-8.6 - Aggregating requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Contracts COMMITTEE FOR PURCHASE FROM PEOPLE WHO ARE BLIND OR SEVERELY DISABLED 8-PUBLIC AVAILABILITY OF..., or a group of requesters acting in concert, is attempting to break a request down into a series...

  5. 41 CFR 51-8.6 - Aggregating requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Contracts COMMITTEE FOR PURCHASE FROM PEOPLE WHO ARE BLIND OR SEVERELY DISABLED 8-PUBLIC AVAILABILITY OF..., or a group of requesters acting in concert, is attempting to break a request down into a series...

  6. 75 FR 48659 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-11

    ... Notice of Proposed Information Collection Requests AGENCY: Department of Education. SUMMARY: The Acting Director, Information Collection Clearance Division, Regulatory Information Management Services, Office of Management, invites comments on the proposed information collection requests as required by the...

  7. 75 FR 50763 - Proposed Agency Information Collection Activities; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-17

    ... Act Submission, supporting statements and approved collection of information instruments are placed... Proposed Agency Information Collection Activities; Comment Request AGENCY: Board of Governors of the... collection of information requests and requirements conducted or sponsored by the Board under conditions...

  8. 42 CFR 405.821 - Request for carrier hearing.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Program § 405.821 Request for carrier hearing. (a) A request for a carrier hearing is any clear expression in writing by a claimant asking for a hearing to adjudicate a claim when not acted upon...

  9. 42 CFR 405.821 - Request for carrier hearing.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Program § 405.821 Request for carrier hearing. (a) A request for a carrier hearing is any clear expression in writing by a claimant asking for a hearing to adjudicate a claim when not acted upon...

  10. Cadmium treatment suppresses DNA polymerase δ catalytic subunit gene expression by acting on the p53 and Sp1 regulatory axis.

    PubMed

    Antoniali, Giulia; Marcuzzi, Federica; Casarano, Elena; Tell, Gianluca

    2015-11-01

    Cadmium (Cd) is a carcinogenic and neurotoxic environmental pollutant. Among the proposed mechanisms for Cd toxic effects, its ability to promote oxidative stress and to inhibit, in vitro, the activities of some Base Excision DNA Repair (BER) enzymes, such as hOGG1, XRCC1 and APE1, have been already established. However, the molecular mechanisms at the basis of these processes are largely unknown especially at sub-lethal doses of Cd and no information is available on the effect of Cd on the expression levels of BER enzymes. Here, we show that non-toxic treatment of neuronal cell lines, with pro-mitogenic doses of Cd, promotes a significant time- and dose-dependent down-regulation of DNA polymerase δ (POLD1) expression through a transcriptional mechanism with a modest effect on Polβ, XRCC1 and APE1. We further elucidated that the observed transcriptional repression on Polδ is acted by through competition by activated p53 on Sp1 at POLD1 promoter and by a squelching effect. We further proved the positive effect of Sp1 not only on POLD1 expression but also on Polβ, XRCC1 and APE1 expression, suggesting that Sp1 has pleiotropic effects on the whole BER pathway. Our results indicated that Cd-mediated impairment of BER pathway, besides acting on the enzymatic functions of some key proteins, is also exerted at the gene expression level of Polδ by acting on the p53-Sp1 regulatory axis. These data may explain not only the Cd-induced neurotoxic effects but also the potential carcinogenicity of this heavy metal.

  11. Prelinguistic Vocalizations Distinguish Pointing Acts

    ERIC Educational Resources Information Center

    Grunloh, Thomas; Liszkowski, Ulf

    2015-01-01

    The current study investigated whether point-accompanying characteristics, like vocalizations and hand shape, differentiate infants' underlying motives of prelinguistic pointing. We elicited imperative (requestive) and declarative (expressive and informative) pointing acts in experimentally controlled situations, and analyzed accompanying…

  12. 10 CFR 1703.105 - Requests for board records not available through the public reading room (FOIA requests).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... addressed to the Designated FOIA Officer and clearly marked “Freedom of Information Act Request.” The... records rapidly. The requester must also state that these facts are true and correct to the best of...

  13. 10 CFR 1703.105 - Requests for board records not available through the public reading room (FOIA requests).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... addressed to the Designated FOIA Officer and clearly marked “Freedom of Information Act Request.” The... records rapidly. The requester must also state that these facts are true and correct to the best of...

  14. No Child Left Behind Act: Improved Accessibility to Education's Information Could Help States Further Implement Teacher Qualification Requirements. Report to Congressional Requesters. GAO-06-25

    ERIC Educational Resources Information Center

    Shaul, Marnie S.

    2005-01-01

    This GAO report examines response of 47 states to the No Child Left Behind Act (NCLBA) of 2001 that established qualification requirements for teachers of core academic subjects that must meet by the end of the 2005-2006 school year. With the deadline approaching for all teachers to meet the requirements, GAO was asked to examine the following:…

  15. Workforce Investment Act: States and Localities Increasingly Coordinate Services for TANF Clients, but Better Information Needed on Effective Approaches. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    Nilsen, Sigurd R.

    The General Accounting Office (GAO) reviewed the state and local coordination of Temporary Assistance for Needy Families (TANF) and Workforce Investment Act (WIA) services for TANF clients. Data were gathered though the following activities: (1) analysis of surveys of WIA officials in all 50 states that were conducted in 2001 and 2000; (2) visits…

  16. Workforce Investment Act: Improvements Needed in Performance Measures To Provide a More Accurate Picture of WIA's Effectiveness. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    Nilsen, Sigurd R.

    A study assessed effectiveness of Workforce Investment Act (WIA) of 1998 performance measures. Data were from a survey of WIA program administrators in 50 states; visits to 5 states; interviews with labor officials and national associations representing state and local workforce development officials; and a document review. Findings indicate…

  17. Native Hawaiian Education Act: Greater Oversight Would Increase Accountability and Enable Targeting of Funds to Areas with Greatest Need. Report to Congressional Requesters. GAO-08-422

    ERIC Educational Resources Information Center

    Government Accountability Office, 2008

    2008-01-01

    The Native Hawaiian Education Act (NHEA) seeks to develop innovative educational programs to assist Native Hawaiians. The Department of Education (Education) administers NHEA and has provided grants for a wide range of activities. Education is authorized to establish a Native Hawaiian Education Council and seven island councils to help implement…

  18. Interim final rules under the Paul Wellstone and Pete Domenici Mental Health Parity and Addiction Equity Act of 2008. Interim final rules with request for comments.

    PubMed

    2010-02-02

    This document contains interim final rules implementing the Paul Wellstone and Pete Domenici Mental Health Parity and Addiction Equity Act of 2008, which requires parity between mental health or substance use disorder benefits and medical/surgical benefits with respect to financial requirements and treatment limitations under group health plans and health insurance coverage offered in connection with a group health plan.

  19. No Child Left Behind Act: Additional Assistance and Research on Effective Strategies Would Help Small Rural Districts. Report to Congressional Requesters. GAO-04-909

    ERIC Educational Resources Information Center

    Hickok, Eugene W.

    2004-01-01

    Congress has raised concerns about difficulties rural school districts face implementing the No Child Left Behind Act (NCLBA). This report describes: key challenges rural states and districts face; strategies rural districts have developed; expenditures and resources related to rural districts? compliance; and guidance and assistance from the…

  20. Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity

    PubMed Central

    Tan, Benedict G.; Wellesley, Frederick C.; Savery, Nigel J.; Szczelkun, Mark D.

    2016-01-01

    The guanine (G)-tract of conserved sequence block 2 (CSB 2) in human mitochondrial DNA can result in transcription termination due to formation of a hybrid G-quadruplex between the nascent RNA and the nontemplate DNA strand. This structure can then influence genome replication, stability and localization. Here we surveyed the frequency of variation in sequence identity and length at CSB 2 amongst human mitochondrial genomes and used in vitro transcription to assess the effects of this length heterogeneity on the activity of the mitochondrial RNA polymerase, POLRMT. In general, increased G-tract length correlated with increased termination levels. However, variation in the population favoured CSB 2 sequences which produced efficient termination while particularly weak or strong signals were avoided. For all variants examined, the 3′ end of the transcripts mapped to the same downstream sequences and were prevented from terminating by addition of the transcription factor TEFM. We propose that CSB 2 length heterogeneity allows variation in the efficiency of transcription termination without affecting the position of the products or the capacity for regulation by TEFM. PMID:27436287

  1. 14 CFR 1212.201 - Requesting a record.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ....201 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Access to Records § 1212.201 Requesting a record. (a) Individuals may request access to their Privacy Act... to their Privacy Act records. This authorization shall be in writing, signed by the individual...

  2. 22 CFR 1101.6 - Requests for access to records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... STATES SECTION PRIVACY ACT OF 1974 § 1101.6 Requests for access to records. (a) Any individual may submit... through Friday (excluding holidays). (c) Inquiries submitted by mail should be marked “PRIVACY ACT REQUEST... made under the Privacy Act. (e) Inquiries concerning whether a system of records contains a...

  3. 22 CFR 1101.6 - Requests for access to records.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... STATES SECTION PRIVACY ACT OF 1974 § 1101.6 Requests for access to records. (a) Any individual may submit... through Friday (excluding holidays). (c) Inquiries submitted by mail should be marked “PRIVACY ACT REQUEST... made under the Privacy Act. (e) Inquiries concerning whether a system of records contains a...

  4. 22 CFR 1101.6 - Requests for access to records.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... STATES SECTION PRIVACY ACT OF 1974 § 1101.6 Requests for access to records. (a) Any individual may submit... through Friday (excluding holidays). (c) Inquiries submitted by mail should be marked “PRIVACY ACT REQUEST... made under the Privacy Act. (e) Inquiries concerning whether a system of records contains a...

  5. 14 CFR 1212.201 - Requesting a record.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....201 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PRIVACY ACT-NASA REGULATIONS Access to Records § 1212.201 Requesting a record. (a) Individuals may request access to their Privacy Act... to their Privacy Act records. This authorization shall be in writing, signed by the individual...

  6. Procedures for the handling of retaliation complaints under section 1558 of the Affordable Care Act. Interim final rule; request for comments.

    PubMed

    2013-02-27

    This document provides the interim final regulations governing the employee protection (whistleblower) provision of section 1558 of the Affordable Care Act, which added section 18C of the Fair Labor Standards Act, to provide protections to employees of health insurance issuers or other employers who may have been subject to retaliation for reporting potential violations of the law's consumer protections (e.g., the prohibition on denials of insurance due to pre-existing conditions) or affordability assistance provisions (e.g., access to health insurance premium tax credits). This interim rule establishes procedures and time frames for the handling of retaliation complaints under section 18C, including procedures and time frames for employee complaints to the Occupational Safety and Health Administration (OSHA), investigations by OSHA, appeals of OSHA determinations to an administrative law judge (ALJ) for a hearing de novo, hearings by ALJs, review of ALJ decisions by the Administrative Review Board (ARB) (acting on behalf of the Secretary of Labor), and judicial review of the Secretary's final decision.

  7. 39 CFR 3004.40 - Hard copy requests for records and for expedited processing.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... FREEDOM OF INFORMATION ACT § 3004.40 Hard copy requests for records and for expedited processing. (a) A... clearly identified as “Freedom of Information Act Request” both in the text of the request and on the... identified as “Expedited Freedom of Information Act Request” both in the text of the request and on...

  8. 39 CFR 3004.40 - Hard copy requests for records and for expedited processing.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... FREEDOM OF INFORMATION ACT § 3004.40 Hard copy requests for records and for expedited processing. (a) A... clearly identified as “Freedom of Information Act Request” both in the text of the request and on the... identified as “Expedited Freedom of Information Act Request” both in the text of the request and on...

  9. 39 CFR 3004.40 - Hard copy requests for records and for expedited processing.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... FREEDOM OF INFORMATION ACT § 3004.40 Hard copy requests for records and for expedited processing. (a) A... clearly identified as “Freedom of Information Act Request” both in the text of the request and on the... identified as “Expedited Freedom of Information Act Request” both in the text of the request and on...

  10. 39 CFR 3004.40 - Hard copy requests for records and for expedited processing.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... FREEDOM OF INFORMATION ACT § 3004.40 Hard copy requests for records and for expedited processing. (a) A... clearly identified as “Freedom of Information Act Request” both in the text of the request and on the... identified as “Expedited Freedom of Information Act Request” both in the text of the request and on...

  11. A particular DNA structure is required for the function of a cis-acting component of the Epstein-Barr virus OriLyt origin of replication.

    PubMed Central

    Portes-Sentis, S; Sergeant, A; Gruffat, H

    1997-01-01

    OriLyt, thecis-acting element of Epstein-Barr virus lytic origin of replication, consists of upstream and downstream components. The upstream component plays a dual role in transcription and replication. The downstream component contains a homopurine-homopyrimidine sequence which forms an H palindrome. We show that the downstream component can adopt a triple helix structure in vitro, that the 5' border of the homopyrimidine sequence is sensitive to P1 nuclease when carried by a supercoiled plasmid and that an oligonucleotide complementary to the homopyrimidine strand is taken up by a plasmid carrying the OriLyt H palindrome. We also show that all mutations which alter the H palindrome impair both oligonucleotide uptake and OriLyt-dependent replication. Interestingly, compensatory mutations which restore an H palindrome also restore oligonucleotide uptake by the mutated plasmids and their OriLyt-dependent replication. Thus, there is a strong correlation between the inability of the OriLyt H palindrome to form a non-B-DNA structure in vitro and impairment of OriLyt-dependent replication. This suggests that the presence of a non-B-DNA structure in the OriLyt downstream component is required for OriLyt-dependent replication. PMID:9060428

  12. DNA adenine methyltransferase (Dam) controls the expression of the cytotoxic enterotoxin (act) gene of Aeromonas hydrophila via tRNA modifying enzyme-glucose-inhibited division protein (GidA).

    PubMed

    Erova, Tatiana E; Kosykh, Valeri G; Sha, Jian; Chopra, Ashok K

    2012-05-01

    Aeromonas hydrophila is both a human and animal pathogen, and the cytotoxic enterotoxin (Act) is a crucial virulence factor of this bacterium because of its associated hemolytic, cytotoxic, and enterotoxic activities. Previously, to define the role of some regulatory genes in modulating Act production, we showed that deletion of a glucose-inhibited division gene (gidA) encoding tRNA methylase reduced Act levels, while overproduction of DNA adenine methyltransferase (Dam) led to a concomitant increase in Act-associated biological activities of a diarrheal isolate SSU of A. hydrophila. Importantly, there are multiple GATC binding sites for Dam within an upstream sequence of the gidA gene and one such target site in the act gene upstream region. We showed the dam gene to be essential for the viability of A. hydrophila SSU, and, therefore, to better understand the interaction of the encoding genes, Dam and GidA, in act gene regulation, we constructed a gidA in-frame deletion mutant of Escherichia coli GM28 (dam(+)) and GM33 (∆dam) strains. We then tested the expressional activity of the act and gidA genes by using a promoterless pGlow-TOPO vector containing a reporter green fluorescent protein (GFP). Our data indicated that in GidA(+) strains of E. coli, constitutive methylation of the GATC site(s) by Dam negatively regulated act and gidA gene expression as measured by GFP production. However, in the ∆gidA strains, irrespective of the presence or absence of constitutively active Dam, we did not observe any alteration in the expression of the act gene signifying the role of GidA in positively regulating Act production. To determine the exact mechanism of how Dam and GidA influence Act, a real-time quantitative PCR (RT-qPCR) assay was performed. The analysis indicated an increase in gidA and act gene expression in the A. hydrophila Dam-overproducing strain, and these data matched with Act production in the E. coli GM28 strain. Thus, the extent of DNA methylation

  13. Glutamine Acts as a Neuroprotectant against DNA Damage, Beta-Amyloid and H2O2-Induced Stress

    PubMed Central

    Chen, Jianmin; Herrup, Karl

    2012-01-01

    Glutamine is the most abundant free amino acid in the human blood stream and is ‘conditionally essential’ to cells. Its intracellular levels are regulated both by the uptake of extracellular glutamine via specific transport systems and by its intracellular synthesis by glutamine synthetase (GS). Adding to the regulatory complexity, when extracellular glutamine is reduced GS protein levels rise. Unfortunately, this excess GS can be maladaptive. GS overexpression is neurotoxic especially if the cells are in a low-glutamine medium. Similarly, in low glutamine, the levels of multiple stress response proteins are reduced rendering cells hypersensitive to H2O2, zinc salts and DNA damage. These altered responses may have particular relevance to neurodegenerative diseases of aging. GS activity and glutamine levels are lower in the Alzheimer's disease (AD) brain, and a fraction of AD hippocampal neurons have dramatically increased GS levels compared with control subjects. We validated the importance of these observations by showing that raising glutamine levels in the medium protects cultured neuronal cells against the amyloid peptide, Aβ. Further, a 10-day course of dietary glutamine supplementation reduced inflammation-induced neuronal cell cycle activation, tau phosphorylation and ATM-activation in two different mouse models of familial AD while raising the levels of two synaptic proteins, VAMP2 and synaptophysin. Together, our observations suggest that healthy neuronal cells require both intracellular and extracellular glutamine, and that the neuroprotective effects of glutamine supplementation may prove beneficial in the treatment of AD. PMID:22413000

  14. 32 CFR 1700.12 - Requests for expedited processing.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... NATIONAL INTELLIGENCE PROCEDURES FOR DISCLOSURE OF RECORDS PURSUANT TO THE FREEDOM OF INFORMATION ACT... statement, certified to be true and correct, explaining in detail the basis for requesting...

  15. 32 CFR 1700.12 - Requests for expedited processing.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... NATIONAL INTELLIGENCE PROCEDURES FOR DISCLOSURE OF RECORDS PURSUANT TO THE FREEDOM OF INFORMATION ACT... statement, certified to be true and correct, explaining in detail the basis for requesting...

  16. 32 CFR 1700.12 - Requests for expedited processing.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... NATIONAL INTELLIGENCE PROCEDURES FOR DISCLOSURE OF RECORDS PURSUANT TO THE FREEDOM OF INFORMATION ACT... statement, certified to be true and correct, explaining in detail the basis for requesting...

  17. 77 FR 52066 - Sunshine Act Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-28

    ... to the Board on the appropriations request for FY 2014 (Resolution 2012-XXX) 4. Consider and act on... 2012-XXX) 6. Consider and act on whether to authorize an executive session of the Board Closed Session... 2012-XXX) 9. Public comment 10. Consider and act on other business 11. Consider and act on motion...

  18. Analysis of S.1844, the Clear Skies Act of 2003; S. 843, the Clean Air Planning Act of 2003; and S. 366, the Clean Power Act of 2003

    EIA Publications

    2004-01-01

    Senator James M. Inhofe requested that the Energy Information Administration (EIA) undertake analysis of S.843, the Clean Air Planning Act of 2003, introduced by Senator Thomas Carper; S.366, the Clean Power Act of 2003, introduced by Senator James Jeffords; and S.1844, the Clear Skies Act of 2003, introduced by Senator James M. Inhofe. The EIA received this request on March 19, 2004. This Service Report responds to his request.

  19. 78 FR 25321 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-30

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Proposed Collection; Comment Request Upon Written Request, Copies Available From: Securities and... Paperwork Reduction Act of 1995 (44 U.S.C. 3501 et seq.), the Securities and Exchange Commission...

  20. 49 CFR 38.37 - Stop request.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false Stop request. 38.37 Section 38.37 Transportation Office of the Secretary of Transportation AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY SPECIFICATIONS FOR TRANSPORTATION VEHICLES Buses, Vans and Systems § 38.37 Stop request. (a) Where passengers...

  1. 49 CFR 38.37 - Stop request.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 1 2014-10-01 2014-10-01 false Stop request. 38.37 Section 38.37 Transportation Office of the Secretary of Transportation AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY SPECIFICATIONS FOR TRANSPORTATION VEHICLES Buses, Vans and Systems § 38.37 Stop request. (a) Where passengers...

  2. 40 CFR 142.41 - Variance request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....41 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS IMPLEMENTATION Variances Issued by the Administrator Under Section 1415(a) of the Act § 142.41 Variance request. A supplier of water may request the granting of...

  3. 40 CFR 142.41 - Variance request.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....41 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS IMPLEMENTATION Variances Issued by the Administrator Under Section 1415(a) of the Act § 142.41 Variance request. A supplier of water may request the granting of...

  4. 40 CFR 142.41 - Variance request.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....41 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS IMPLEMENTATION Variances Issued by the Administrator Under Section 1415(a) of the Act § 142.41 Variance request. A supplier of water may request the granting of...

  5. 75 FR 70309 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Proposed Collection; Comment Request Upon Written Request, Copies Available From: U.S. Securities... pursuant to the Paperwork Reduction Act of 1995 (44 U.S.C. 3501 et seq.), the Securities and...

  6. 75 FR 10529 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Proposed Collection; Comment Request Upon Written Request, Copies Available From: Securities and... given that, pursuant to the Paperwork Reduction Act of 1995 (44 U.S.C. 3501 et seq.), the Securities...

  7. 77 FR 59225 - Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    ... Reduction Act of 1995 (PRA95) . This program helps to ensure that requested data can be provided in the... clearly understood, and the impact of collection requirements on respondents can be properly assessed. The... ``International Training Application.'' A copy of the proposed information collection request (ICR) can...

  8. 75 FR 69128 - Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-10

    ... Reduction Act of 1995 (PRA95) . This program helps to ensure that requested data can be provided in the... clearly understood, and the impact of collection requirements on respondents can be properly assessed. The... information collection request (ICR) can be obtained by contacting the individual listed below in...

  9. 75 FR 55356 - Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-10

    ... Reduction Act of 1995 (PRA95) . This program helps to ensure that requested data can be provided in the... clearly understood, and the impact of collection requirements on respondents can be properly assessed. The... ``Telephone Point of Purchase Survey.'' A copy of the proposed information collection request (ICR) can...

  10. 77 FR 27799 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... Reduction Act of 1995 (PRA95) . This program helps to ensure that requested data can be provided in the... clearly understood, and the impact of collection requirements on respondents can be properly assessed. The... collection request (ICR) can be obtained by contacting the individual listed below in the Addresses...

  11. 77 FR 2094 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-13

    ... Reduction Act of 1995 (PRA95) . This program helps to ensure that requested data can be provided in the... clearly understood, and the impact of collection requirements on respondents can be properly assessed... information collection request can be obtained by contacting the office listed below in the address section...

  12. 77 FR 5090 - Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-01

    ... Bureau of the Public Debt Proposed Collection: Comment Request ACTION: Notice and request for comments... on proposed and/or continuing information collections, as required by the Paperwork Reduction Act of... on: (a) Whether the collection of information is necessary for the proper performance of...

  13. 78 FR 25533 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... Bureau of the Public Debt Proposed Collection; Comment Request ACTION: Notice and request for comments... on proposed and/or continuing information collections, as required by the Paperwork Reduction Act of... on: (a) Whether the collection of information is necessary for the proper performance of...

  14. 77 FR 72893 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-06

    ... COMMISSION Proposed Collection; Comment Request Upon Written Request Copies Available From: Securities and... Exchange Commission (``Commission'') is soliciting comments on the existing collection of information... Exchange Act of 1934 (15 U.S.C. 78a et seq.). The Commission plans to submit this existing collection...

  15. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of identity,...

  16. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  17. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  18. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of...

  19. 12 CFR 1102.106 - Review of requests for amendment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 9 2012-01-01 2012-01-01 false Review of requests for amendment. 1102.106....S.C. 552(e) (1) and (5), i.e., that records maintained by the ASC shall contain only such... information from a record. (c) Time for acting on requests. Initial review of a request by an individual...

  20. 28 CFR 700.20 - Requests for correction of records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Requests for correction of records. 700... Records Under the Privacy Act of 1974 § 700.20 Requests for correction of records. (a) How made. Unless a record is exempted from correction and amendment, an individual may submit a request for correction of...

  1. 28 CFR 700.20 - Requests for correction of records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Requests for correction of records. 700... Records Under the Privacy Act of 1974 § 700.20 Requests for correction of records. (a) How made. Unless a record is exempted from correction and amendment, an individual may submit a request for correction of...

  2. 36 CFR 1275.70 - Freedom of information requests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requests. (a) The Archivist will process Freedom of Information Act requests for access to only those materials within the Presidential historical materials which are identifiable by an archivist as records of an agency as defined in § 1275.16(f). The Archivist will process these requests in accordance...

  3. 36 CFR 1275.70 - Freedom of information requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requests. (a) The Archivist will process Freedom of Information Act requests for access to only those materials within the Presidential historical materials which are identifiable by an archivist as records of an agency as defined in § 1275.16(f). The Archivist will process these requests in accordance...

  4. 22 CFR 308.15 - Identification of requesters.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Identification of requesters. 308.15 Section 308.15 Foreign Relations PEACE CORPS IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 308.15 Identification of requesters. The agency shall require reasonable identification of all individuals who request access...

  5. 22 CFR 308.15 - Identification of requesters.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 2 2012-04-01 2009-04-01 true Identification of requesters. 308.15 Section 308.15 Foreign Relations PEACE CORPS IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 308.15 Identification of requesters. The agency shall require reasonable identification of all individuals who request access...

  6. 22 CFR 308.15 - Identification of requesters.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Identification of requesters. 308.15 Section 308.15 Foreign Relations PEACE CORPS IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 308.15 Identification of requesters. The agency shall require reasonable identification of all individuals who request access...

  7. 50 CFR 501.5 - Disclosure of requested information.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Disclosure of requested information. 501.5 Section 501.5 Wildlife and Fisheries MARINE MAMMAL COMMISSION IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 501.5 Disclosure of requested information. (a) Upon request and satisfactory proof of identity,...

  8. 22 CFR 707.23 - Requests for amendment of records.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... must be in writing, labeled “Privacy Act Request,” and should be addressed to the Director of Human...) Information to include. All requests under this section must: (1) Be in writing and be signed by the requester... records and provide a record creation time range. A description of OPIC's systems of records can...

  9. 11 CFR 9405.7 - Requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Federal Elections ELECTION ASSISTANCE COMMISSION PROCEDURES FOR DISCLOSURE OF RECORDS UNDER THE FREEDOM OF INFORMATION ACT § 9405.7 Requests for records. (a) Requests for copies of Commission records under the FOIA..., 1201 New York Avenue, NW., Suite 300, Washington, DC 20005. The request shall reasonably describe...

  10. 11 CFR 9405.7 - Requests for records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Federal Elections ELECTION ASSISTANCE COMMISSION PROCEDURES FOR DISCLOSURE OF RECORDS UNDER THE FREEDOM OF INFORMATION ACT § 9405.7 Requests for records. (a) Requests for copies of Commission records under the FOIA..., 1201 New York Avenue, NW., Suite 300, Washington, DC 20005. The request shall reasonably describe...

  11. 11 CFR 9405.7 - Requests for records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Federal Elections ELECTION ASSISTANCE COMMISSION PROCEDURES FOR DISCLOSURE OF RECORDS UNDER THE FREEDOM OF INFORMATION ACT § 9405.7 Requests for records. (a) Requests for copies of Commission records under the FOIA..., 1201 New York Avenue, NW., Suite 300, Washington, DC 20005. The request shall reasonably describe...

  12. 11 CFR 9405.7 - Requests for records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Federal Elections ELECTION ASSISTANCE COMMISSION PROCEDURES FOR DISCLOSURE OF RECORDS UNDER THE FREEDOM OF INFORMATION ACT § 9405.7 Requests for records. (a) Requests for copies of Commission records under the FOIA..., 1201 New York Avenue, NW., Suite 300, Washington, DC 20005. The request shall reasonably describe...

  13. 11 CFR 9405.7 - Requests for records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Federal Elections ELECTION ASSISTANCE COMMISSION PROCEDURES FOR DISCLOSURE OF RECORDS UNDER THE FREEDOM OF INFORMATION ACT § 9405.7 Requests for records. (a) Requests for copies of Commission records under the FOIA..., 1225 New York Avenue, NW., Suite 1100, Washington, DC 20005. The request shall reasonably describe...

  14. 18 CFR 1301.6 - Responses to requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true Responses to requests. 1301.6 Section 1301.6 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.6 Responses to requests. (a) Acknowledgements of requests. On receipt...

  15. 18 CFR 1301.6 - Responses to requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Responses to requests. 1301.6 Section 1301.6 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.6 Responses to requests. (a) Acknowledgements of requests. On receipt...

  16. 18 CFR 1301.6 - Responses to requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 2 2012-04-01 2012-04-01 false Responses to requests. 1301.6 Section 1301.6 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.6 Responses to requests. (a) Acknowledgements of requests. On receipt...

  17. 18 CFR 1301.6 - Responses to requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false Responses to requests. 1301.6 Section 1301.6 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.6 Responses to requests. (a) Acknowledgements of requests. On receipt...

  18. 18 CFR 1301.6 - Responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Responses to requests. 1301.6 Section 1301.6 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.6 Responses to requests. (a) Acknowledgements of requests. On receipt...

  19. 5 CFR 2100.7 - Request for correction or amendment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Request for correction or amendment. 2100.7 Section 2100.7 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.7 Request for correction or amendment. (a) Requests to correct or amend...

  20. 5 CFR 2100.7 - Request for correction or amendment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Request for correction or amendment. 2100.7 Section 2100.7 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.7 Request for correction or amendment. (a) Requests to correct or amend...

  1. 5 CFR 2100.7 - Request for correction or amendment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Request for correction or amendment. 2100.7 Section 2100.7 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.7 Request for correction or amendment. (a) Requests to correct or amend...

  2. 5 CFR 9301.6 - Requesting records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... INFORMATION Freedom of Information Act Procedures for Disclosure of Records Under the Freedom of Information... statement, which shall state that it is true and correct to the best of the requester's knowledge and...

  3. 78 FR 72065 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... of the Secretary Proposed Collection; Comment Request AGENCY: Department of Defense Education... Act of 1995, the Department of Defense Education Activity announces a proposed public information... collection on respondents, including through the use of automated collection techniques or other forms...

  4. 5 CFR 1302.2 - Requests for access.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....2 Administrative Personnel OFFICE OF MANAGEMENT AND BUDGET ADMINISTRATIVE PROCEDURES PRIVACY ACT... the Privacy Act of 1974. In the absence of specifying solely the Privacy Act of 1974 and, if the request may be processed under both the Freedom of Information Act and the Privacy Act and the...

  5. 5 CFR 1302.2 - Requests for access.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ....2 Administrative Personnel OFFICE OF MANAGEMENT AND BUDGET ADMINISTRATIVE PROCEDURES PRIVACY ACT... the Privacy Act of 1974. In the absence of specifying solely the Privacy Act of 1974 and, if the request may be processed under both the Freedom of Information Act and the Privacy Act and the...

  6. 5 CFR 1302.2 - Requests for access.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ....2 Administrative Personnel OFFICE OF MANAGEMENT AND BUDGET ADMINISTRATIVE PROCEDURES PRIVACY ACT... the Privacy Act of 1974. In the absence of specifying solely the Privacy Act of 1974 and, if the request may be processed under both the Freedom of Information Act and the Privacy Act and the...

  7. 48 CFR 926.7003 - Review of the procurement request.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... potential for making Energy Policy Act awards. ... request. 926.7003 Section 926.7003 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SOCIOECONOMIC PROGRAMS OTHER SOCIOECONOMIC PROGRAMS Implementation of Section 3021 of the Energy Policy Act...

  8. 48 CFR 926.7003 - Review of the procurement request.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... potential for making Energy Policy Act awards. ... request. 926.7003 Section 926.7003 Federal Acquisition Regulations System DEPARTMENT OF ENERGY SOCIOECONOMIC PROGRAMS OTHER SOCIOECONOMIC PROGRAMS Implementation of Section 3021 of the Energy Policy Act...

  9. TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is a potential prognostic biomarker in lung cancer.

    PubMed

    Liu, Wen-bin; Han, Fei; Jiang, Xiao; Chen, Hong-qiang; Zhao, Huan; Liu, Yong; Li, Yong-hong; Huang, Chuanshu; Cao, Jia; Liu, Jin-yi

    2015-08-28

    Epigenetic silencing of tumour suppressors contributes to the development and progression of lung cancer. We recently found that TMEM196 was hypermethylated in lung cancer. This study aimed to clarify its epigenetic regulation, possible roles and clinical significance. TMEM196 methylation correlated with loss of protein expression in chemical-induced rat lung pathologic lesions and human lung cancer tissues and cell lines. 5-aza-2'-deoxycytidine restored TMEM196 expression. Moreover, TMEM196 hypermethylation was detected in 61.2% of primary lung tumours and found to be associated with poor differentiation and pathological stage of lung cancer. Functional studies showed that ectopic re-expression of TMEM196 in lung cancer cells inhibited cell proliferation, clonogenicity, cell motility and tumour formation. However, TMEM196 knockdown increased cell proliferation and inhibited apoptosis and cell-cycle arrest. These effects were associated with upregulation of p21 and Bax, and downregulation of cyclin D1, c-myc, CD44 and β-catenin. Kaplan-Meier survival curves showed that TMEM196 downregulation was significantly associated with shortened survival in lung cancer patients. Multivariate analysis showed that patients with TMEM196 expression had a better overall survival. Our results revealed for the first time that TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is an independent prognostic factor of lung cancer.

  10. 36 CFR 1008.15 - Requests for access to records: Initial decision.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... records: Initial decision. 1008.15 Section 1008.15 Parks, Forests, and Public Property PRESIDIO TRUST REQUESTS UNDER THE PRIVACY ACT § 1008.15 Requests for access to records: Initial decision. (a) Decisions on requests. A request made under this part for access to a record shall be granted promptly unless the...

  11. 43 CFR 2.23 - When will the bureau deny a request or procedural benefits?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... requested record is exempt, in full or in part; (2) The request does not reasonably describe the records sought; (3) A requested record does not exist, cannot be located, or is not in the bureau's possession... OF INFORMATION ACT; RECORDS AND TESTIMONY Responses to Requests § 2.23 When will the bureau deny...

  12. Rapid DNA Act of 2014

    THOMAS, 113th Congress

    Rep. Sensenbrenner, F. James, Jr. [R-WI-5

    2014-12-03

    12/18/2014 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, and Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  13. 29 CFR 2702.3 - Requests for information.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... IMPLEMENTING THE FREEDOM OF INFORMATION ACT § 2702.3 Requests for information. (a) Content of request. All... submitted electronically or by facsimile. The words “Freedom of Information Act Request” should be printed... processing shall be made by a statement certified by such person to be true and correct to the best of...

  14. 29 CFR 2702.3 - Requests for information.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... IMPLEMENTING THE FREEDOM OF INFORMATION ACT § 2702.3 Requests for information. (a) Content of request. All... submitted electronically or by facsimile. The words “Freedom of Information Act Request” should be printed... processing shall be made by a statement certified by such person to be true and correct to the best of...

  15. 22 CFR 503.2 - Making a request.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Making a request. 503.2 Section 503.2 Foreign Relations BROADCASTING BOARD OF GOVERNORS FREEDOM OF INFORMATION ACT REGULATION § 503.2 Making a request. (a... addressed to The Broadcasting Board of Governors (BBG), FOIA/Privacy Act Officer, Office of the...

  16. 4 CFR 200.5 - Requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4 Accounts 1 2010-01-01 2010-01-01 false Requests for access to records. 200.5 Section 200.5 Accounts RECOVERY ACCOUNTABILITY AND TRANSPARENCY BOARD PRIVACY ACT OF 1974 § 200.5 Requests for access to...: Privacy Act Officer, Recovery Accountability and Transparency Board, 1717 Pennsylvania Avenue, NW.,...

  17. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  18. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  19. 10 CFR 1304.109 - Requests for correction of records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Requests for correction of records. 1304.109 Section 1304.109 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.109 Requests for correction... Act Officer; U.S. Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300;...

  20. 14 CFR 1206.701 - Categories of requesters.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... RECORDS TO MEMBERS OF THE PUBLIC Search, Review, and Duplication Fees § 1206.701 Categories of requesters... scientific institutions; representatives of the news media; and all other requesters. The Act prescribes... under the fee provisions of the Privacy Act of 1974, which permits fees only for...

  1. 36 CFR 1600.22 - Requests for access to records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Privacy and Access to Individual Records Under the Privacy Act of 1974 § 1600.22 Requests for.... For the quickest possible handling, you should mark both your request letter and the envelope “Privacy... of the “Privacy Act Compilation” published by the National Archives and Records...

  2. 5 CFR 293.310 - Response to requests for information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... cites the FOIA, as with all stated Privacy Act requests made by current employees, shall be processed in accordance with agency Privacy Act procedures consistent with Office regulations in part 297 of this chapter... be referred to the Office's regional or area office nearest to the location of the requester....

  3. 5 CFR 293.310 - Response to requests for information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... cites the FOIA, as with all stated Privacy Act requests made by current employees, shall be processed in accordance with agency Privacy Act procedures consistent with Office regulations in part 297 of this chapter... be referred to the Office's regional or area office nearest to the location of the requester....

  4. 36 CFR 1600.22 - Requests for access to records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Protection of Privacy and Access to Individual Records Under the Privacy Act of 1974 § 1600.22 Requests for.... For the quickest possible handling, you should mark both your request letter and the envelope “Privacy... of the “Privacy Act Compilation” published by the National Archives and Records...

  5. Food security of older adults requesting Older Americans Act Nutrition Program in Georgia can be validly measured using a short form of the U.S. Household Food Security Survey Module.

    PubMed

    Lee, Jung Sun; Johnson, Mary Ann; Brown, Arvine; Nord, Mark

    2011-07-01

    Food security is a newly recommended outcome measure for the Older Americans Act Nutrition Program (OAANP); however, it is unknown how best to evaluate the need for this program and assess its impact on a large scale. Therefore, we measured food security in all new OAANP participants and waitlisted applicants in Georgia between July and early November, 2008 (n = 4731) with the self-administered mail survey method used in the ongoing Georgia Performance Outcomes Measures project. We used a modified 6-item U.S. Household Food Security Survey Module (HFSSM) with a 30-d reference period and 2 reminder postcards. Approximately 33% of those identified completed the survey (n = 1594, mean age 74.6 ± 9.5 y, 68.6% female, 30.6% black). Most of the respondents (91%) completed all 6 food security questions, whereas 26 did not respond to any question. Infit and outfit statistics for each of the 6 questions were within an acceptable range. Psychometric properties observed in our food security data were generally similar to those in the nationally representative survey conducted by the Census Bureau and suggest that our food security statistics may be meaningfully compared with national food security statistics published by the USDA. Our findings suggest that food security can be reasonably measured by a short form of HFSSM in older adults requesting OAANP. Such methodology also can be used to estimate the extent of food insecurity and help guide program and policy decisions to meet the nutrition assistance needs of vulnerable older adults.

  6. 76 FR 76165 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-06

    ... Public Service Announcement (PSA) campaign that aims to educate, engage and empower women and their... HUMAN SERVICES Agency Information Collection Request. 60-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  7. 77 FR 13128 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ...'' campaign is a national Public Service Announcement (PSA) campaign that aims to educate, engage and empower... HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  8. 77 FR 37407 - Agency Information Collection Request-30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-21

    ... HUMAN SERVICES Agency Information Collection Request--30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is.... Proposed Project: New Comprehensive Communication Campaign on Right To Non-Discrimination in Certain...

  9. 77 FR 18820 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services,...

  10. 76 FR 59129 - Agency Information Collection Request; 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... HUMAN SERVICES Agency Information Collection Request; 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... to send comments regarding this burden estimate or any other aspect of this collection of...

  11. 77 FR 32638 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-01

    ... HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is...) ways to enhance the quality, utility, and clarity of the information to be collected; and (4) the...

  12. 76 FR 79683 - Agency Information Collection Request. 30-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... HUMAN SERVICES Agency Information Collection Request. 30-Day Public Comment Request AGENCY: Office of... Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services, is... new AIDS cases. As one strategy to address this disparity, the U.S. Department of Health and...

  13. 76 FR 36131 - Agency Information Collection Request. 60-Day Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ... HUMAN SERVICES ; 60-Day Notice] Agency Information Collection Request. 60-Day Public Comment Request... Paperwork Reduction Act of 1995, the Office of the Secretary (OS), Department of Health and Human Services... and Human Services Office of HIV/AIDS Policy (OHAP) implemented the Minority Serving...

  14. 78 FR 13472 - Freedom of Information Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... CFR Part 4 Freedom of Information Act AGENCY: Federal Trade Commission (FTC). ACTION: Final rule... information for the filing of initial Freedom of Information Act (``FOIA'') requests; clarifying agency..., Freedom of Information Act. For the reasons set forth in the preamble, the Federal Trade Commission...

  15. 76 FR 44962 - Sunshine Act Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-27

    ... 335, Harvard Law School, 1563 Massachusetts Avenue, Cambridge, MA 02138. PUBLIC OBSERVATION: Members...'s FY 2013 appropriation request. 4. Public comment. 5. Consider and act on other business. 6... business days in advance of the meeting. If a request is made without advance notice, LSC will make...

  16. Freedom of Information Act

    USGS Publications Warehouse

    Newman, D.J.

    2012-01-01

    The Freedom of Information Act( FOIA), 5 U.S.C.§ 552, as amended, generally provides that any person has a right to request access to Federal agency records. The USGS proactively promotes information disclosure as inherent to its mission of providing objective science to inform decisionmakers and the general public. USGS scientists disseminate up-to-date and historical scientific data that are critical to addressing national and global priorities.

  17. 77 FR 75147 - Proposed Information Collection; Comment Request; Interim Procedures for Considering Requests...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-19

    ... (``CITA''), which issues procedures and acts on requests through the U.S. Department of Commerce, Office... public dissemination of information used by CITA in making commercial availability determinations....

  18. Detection of cis- and trans-acting Factors in DNA Structure-Induced Genetic Instability Using In silico and Cellular Approaches

    PubMed Central

    Wang, Guliang; Zhao, Junhua; Vasquez, Karen M.

    2016-01-01

    Sequences that can adopt alternative DNA structures (i.e., non-B DNA) are very abundant in mammalian genomes, and recent studies have revealed many important biological functions of non-B DNA structures in chromatin remodeling, DNA replication, transcription, and genetic instability. Here, we provide results from an in silico web-based search engine coupled with cell-based experiments to characterize the roles of non-B DNA conformations in genetic instability in eukaryotes. The purpose of this article is to illustrate strategies that can be used to identify and interrogate the biological roles of non-B DNA structures, particularly on genetic instability. We have included unpublished data using a short H-DNA-forming sequence from the human c-MYC promoter region as an example, and identified two different mechanisms of H-DNA-induced genetic instability in yeast and mammalian cells: a DNA replication-related model of mutagenesis; and a replication-independent cleavage model. Further, we identified candidate proteins involved in H-DNA-induced genetic instability by using a yeast genetic screen. A combination of in silico and cellular methods, as described here, should provide further insight into the contributions of non-B DNA structures in biological functions, genetic evolution, and disease development. PMID:27532010

  19. 78 FR 68096 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Quick...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... Workforce Investment Act Implementation AGENCY: Office of the Secretary, DOL. ACTION: Notice. SUMMARY: The... information collection request (ICR) titled, ``Quick Turnaround Surveys on Workforce Investment Act... quick turnaround surveys on Workforce Investment Act (WIA) related issues. Specifically, this ICR is...

  20. 77 FR 77112 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Workforce...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ...; Workforce Investment Act Management Information and Reporting System ACTION: Notice. SUMMARY: The Department... Collection: Workforce Investment Act Management Information and Reporting System. OMB Control Number: 1205... collection request (ICR) revision titled, ``Workforce Investment Act Management Information and...

  1. 31 CFR 50.9 - Procedure for requesting general interpretations of statute.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... TERRORISM RISK INSURANCE PROGRAM General Provisions § 50.9 Procedure for requesting general interpretations... request an interpretation of the Act or regulations by writing to the Terrorism Risk Insurance...

  2. Foreign Evidence Request Efficiency Act of 2009

    THOMAS, 111th Congress

    Rep. Schiff, Adam B. [D-CA-29

    2009-07-08

    08/19/2009 Referred to the Subcommittee on Crime, Terrorism, and Homeland Security. (All Actions) Notes: For further action, see S.1289, which became Public Law 111-79 on 10/19/2009. Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  3. 77 FR 58398 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-20

    ...: Title IV-E Plan for Foster Care, Adoption Assistance, and, optional, Guardianship Assistance Programs... Social Security Act (the Act) for each public child welfare agency requesting Federal funding for foster care, adoption assistance and guardianship assistance under the Act. Section 479B of the Act...

  4. 77 FR 65248 - Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-25

    ... or Check Issued Under the Armed Forces Leave Act of 1946, as amended. DATES: Written comments should... Bond or Check Issued Under the Armed Forces Leave Act of 1946, as amended. OMB Number: 1535-0104. Form Number: PD F 2066. Abstract: The information is requested to support payment of an Armed Forces...

  5. 76 FR 28962 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... AF A System Name: Information requests--Freedom of Information Act (April 7, 2003, 68 FR 16787... requested records from the Air Force under the provisions of the Freedom of Information Act (FOIA); all... foregoing is true and correct. Executed on (date). (Signature)'. If executed within the United States,...

  6. 77 FR 61407 - Agency Forms Undergoing Paperwork Reduction Act Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-09

    ... HUMAN SERVICES Centers for Disease Control and Prevention Agency Forms Undergoing Paperwork Reduction Act Review The Centers for Disease Control and Prevention (CDC) publishes a list of information... Paperwork Reduction Act (44 U.S.C. Chapter 35). To request a copy of these requests, call the CDC...

  7. Female EFL Learners' Request Realization in Relation to Their Ethnic Backgrounds

    ERIC Educational Resources Information Center

    Sari, Artanti Puspita

    2010-01-01

    The choice of strategies and modifications of speech acts in conversational communication may be correlated with sociolinguistic factors, such as ethnicity, social distance and dominance. Requests, as a part of speech acts, provide an interesting focus in this research because requests are face-threatening acts. This article was based on a…

  8. Human Mre11/human Rad50/Nbs1 and DNA ligase IIIalpha/XRCC1 protein complexes act together in an alternative nonhomologous end joining pathway.

    PubMed

    Della-Maria, Julie; Zhou, Yi; Tsai, Miaw-Sheue; Kuhnlein, Jeff; Carney, James P; Paull, Tanya T; Tomkinson, Alan E

    2011-09-30

    Recent studies have implicated a poorly defined alternative pathway of nonhomologous end joining (alt-NHEJ) in the generation of large deletions and chromosomal translocations that are frequently observed in cancer cells. Here, we describe an interaction between two factors, hMre11/hRad50/Nbs1 (MRN) and DNA ligase IIIα/XRCC1, that have been linked with alt-NHEJ. Expression of DNA ligase IIIα and the association between MRN and DNA ligase IIIα/XRCC1 are altered in cell lines defective in the major NHEJ pathway. Most notably, DNA damage induced the association of these factors in DNA ligase IV-deficient cells. MRN interacts with DNA ligase IIIα/XRCC1, stimulating intermolecular ligation, and together these proteins join incompatible DNA ends in a reaction that mimics alt-NHEJ. Thus, our results provide novel mechanistic insights into the alt-NHEJ pathway that not only contributes to genome instability in cancer cells but may also be a therapeutic target.

  9. P-glycoprotein attenuates DNA repair activity in multidrug-resistant cells by acting through the Cbp-Csk-Src cascade.

    PubMed

    Lin, Li-Fang; Wu, Ming-Hsi; Pidugu, Vijaya Kumar; Ho, I-Ching; Su, Tsann-Long; Lee, Te-Chang

    2017-02-03

    Recent studies have demonstrated that P-glycoprotein (P-gp) expression impairs DNA interstrand cross-linking agent-induced DNA repair efficiency in multidrug-resistant (MDR) cells. To date, the detailed molecular mechanisms underlying how P-gp interferes with Src activation and subsequent DNA repair activity remain unclear. In this study, we determined that the C-terminal Src kinase-binding protein (Cbp) signaling pathway involved in the negative control of Src activation is enhanced in MDR cells. We also demonstrated that cells that ectopically express P-gp exhibit reduced activation of DNA damage response regulators, such as ATM, Chk2, Braca1 and Nbs1 and hence attenuated DNA double-strand break repair capacity and become more susceptible than vector control cells to DNA interstrand cross-linking (ICL) agents. Moreover, we demonstrated that P-gp can not only interact with Cbp and Src but also enhance the formation of inhibitory C-terminal Src kinase (Csk)-Cbp complexes that reduce phosphorylation of the Src activation residue Y416 and increase phosphorylation of the Src negative regulatory residue Y527. Notably, suppression of Cbp expression in MDR cells restores cisplatin-induced Src activation, improves DNA repair capacity, and increases resistance to ICL agents. Ectopic expression of Cbp attenuates cisplatin-induced Src activation and increases the susceptibility of cells to ICL agents. Together, the current results indicate that P-gp inhibits DNA repair activity by modulating Src activation via Cbp-Csk-Src cascade. These results suggest that DNA ICL agents are likely to have therapeutic potential against MDR cells with P-gp-overexpression.

  10. Collaborative Dialogue in Learning Pragmatics: Pragmatic-Related Episodes as an Opportunity for Learning Request-Making

    ERIC Educational Resources Information Center

    Taguchi, Naoko; Kim, Youjin

    2016-01-01

    This study examined the effects of collaborative dialogue in learning the speech act of request. Seventy-four second-grade girls' junior high students were divided into three groups. The "collaborative group" (n = 25) received explicit metapragmatic information on request (request head act and modifications) followed by a dialogue…

  11. "Self-activating" chemical nuclease: ferrocenyl cyclen Cu(II) complexes act as efficient DNA cleavage reagents in the absence of reductant.

    PubMed

    Li, Kun; Zhou, Li-Hong; Zhang, Ji; Chen, Shan-Yong; Zhang, Zhong-Wei; Zhang, Jing-Jing; Lin, Hong-Hui; Yu, Xiao-Qi

    2009-04-01

    The interactions of cyclen Cu(II) complexes functionalized by ferrocenyl group with plasmid DNA indicated that these complexes have high cleavage efficiency via an oxidative mechanism in the absence of any reductant or oxidant.

  12. A novel cis-acting element from the 3'UTR of DNA damage-binding protein 2 mRNA links transcriptional and post-transcriptional regulation of gene expression.

    PubMed

    Melanson, Brian D; Cabrita, Miguel A; Bose, Reetesh; Hamill, Jeffrey D; Pan, Elysia; Brochu, Christian; Marcellus, Kristen A; Zhao, Tong T; Holcik, Martin; McKay, Bruce C

    2013-06-01

    The DNA damage-binding protein 2 (DDB2) is an adapter protein that can direct a modular Cul4-DDB1-RING E3 Ligase complex to sites of ultraviolet light-induced DNA damage to ubiquitinate substrates during nucleotide excision repair. The DDB2 transcript is ultraviolet-inducible; therefore, its regulation is likely important for its function. Curiously, the DDB2 mRNA is reportedly short-lived, but the transcript does not contain any previously characterized cis-acting determinants of mRNA stability in its 3' untranslated region (3'UTR). Here, we used a tetracycline regulated d2EGFP reporter construct containing specific 3'UTR sequences from DDB2 to identify novel cis-acting elements that regulate mRNA stability. Synthetic 3'UTRs corresponding to sequences as short as 25 nucleotides from the central region of the 3'UTR of DDB2 were sufficient to accelerate decay of the heterologous reporter mRNA. Conversely, these same 3'UTRs led to more rapid induction of the reporter mRNA, export of the message to the cytoplasm and the subsequent accumulation of the encoded reporter protein, indicating that this newly identified cis-acting element affects transcriptional and post-transciptional processes. These results provide clear evidence that nuclear and cytoplasmic processing of the DDB2 mRNA is inextricably linked.

  13. 10 CFR 1304.103 - Privacy Act inquiries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Privacy Act inquiries. 1304.103 Section 1304.103 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.103 Privacy Act inquiries. (a) Requests... writing may be sent to: Privacy Act Officer, U.S. Nuclear Waste Technical Review Board, 2300...

  14. Privacy Act

    EPA Pesticide Factsheets

    Learn about the Privacy Act of 1974, the Electronic Government Act of 2002, the Federal Information Security Management Act, and other information about the Environmental Protection Agency maintains its records.

  15. 78 FR 76859 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Prevent All...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... Collection; Comments Requested: Prevent All Cigarette Trafficking (PACT) Act Registration Form ACTION: 60-Day... a currently approved collection. (2) The title of the form/collection: Prevent All...

  16. 78 FR 5169 - Proposed Information Collection; Comment Request; Procedures for Considering Requests and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-24

    ... Requests and Comments From the Public for Textile and Apparel Safeguard Actions on Imports From Oman AGENCY... to Maria D'Andrea, Office of Textiles and Apparel, U.S. Department of Commerce, Tel. (202) 482-1550... Act (the ``Act'') implements the textile and apparel safeguard provisions, provided for in Article...

  17. 78 FR 48434 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... Water Act (CWA), as amended by the Oil Pollution Act of 1990. The Schedule is used by federal On-Scene... Oil and Hazardous Substances Pollution Contingency Plans (Renewal) AGENCY: Environmental Protection... collection request (ICR), National Oil and Hazardous Substances Pollution Contingency Plans (Renewal)...

  18. 45 CFR 2106.5 - Requests to amend records.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Commission of Fine Arts whether to grant in full, or deny any part of the request to amend the record; (ii... 45 Public Welfare 4 2014-10-01 2014-10-01 false Requests to amend records. 2106.5 Section 2106.5... COMPLIANCE WITH 5 U.S.C. 552a, THE PRIVACY ACT OF 1974 § 2106.5 Requests to amend records. (a)...

  19. 45 CFR 2106.5 - Requests to amend records.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Commission of Fine Arts whether to grant in full, or deny any part of the request to amend the record; (ii... 45 Public Welfare 4 2011-10-01 2011-10-01 false Requests to amend records. 2106.5 Section 2106.5... COMPLIANCE WITH 5 U.S.C. 552a, THE PRIVACY ACT OF 1974 § 2106.5 Requests to amend records. (a)...

  20. 77 FR 57013 - Privacy Act; Implementation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... of the Secretary 32 CFR Part 319 Privacy Act; Implementation AGENCY: Defense Intelligence Agency, DoD. ACTION: Direct final rule with request for comments. SUMMARY: The Defense Intelligence Agency is updating the Defense Intelligence Agency Privacy Act Program, by adding the (k)(2) exemption to...

  1. Reverse transcription - 3' rapid amplification of cDNA ends-nested PCR of ACT1 and SAP2 mRNA as a means of detecting viable Candida albicans in an in vitro cutaneous candidiasis model.

    PubMed

    Okeke, C N; Tsuboi, R; Kawai, M; Yamazaki, M; Reangchainam, S; Ogawa, H

    2000-01-01

    The presence of viable cells of Candida albicans, in broth or in a reconstructed living skin equivalent, was determined by the detection of amplicons of partial mRNA sequences of the genes encoding fungal actin (ACT1) and secreted aspartyl proteinase 2 (SAP2). The mRNA of both genes were amplified by reverse transcription-3' rapid amplification of cDNA ends-nested polymerase chain reaction. Single bands of ACT1 (315 bp) and SAP2 (162 bp) mRNA were amplified from total RNA extracts of C. albicans grown in yeast carbon base-albumin broth or in living skin equivalent tissue; only the former was amplified from Sabouraud broth-grown organisms. Primer pairs targeted for ACT1 and SAP2 were Candida genus-specific and C. albicans-specific, respectively. The sensitivity limits of the assay were 100 fg of total RNA or 10 cells of C. albicans, by ethidium bromide staining. When C. albicans-infected living skin equivalent was exposed to amorolfine, amplicons of ACT1 and SAP2 mRNA were not detected in total RNA extracts. Non-amplification of the mRNA correlated with the absence of C. albicans growth in Sabouraud agar cultures of living skin equivalent samples. Reverse transcription-3' rapid amplification of cDNA ends-nested polymerase chain reaction of the mRNA encoding specific proteins of an organism has potential application in determining the viability of the organism in tissue, thus monitoring the efficacy of an antimicrobial therapy, and in detecting mRNA expressed in very little amounts in tissue.

  2. Nuclear respiratory factor 2 induces the expression of many but not all human proteins acting in mitochondrial DNA transcription and replication.

    PubMed

    Bruni, Francesco; Polosa, Paola Loguercio; Gadaleta, Maria Nicola; Cantatore, Palmiro; Roberti, Marina

    2010-02-05

    In mammals, NRF-2 (nuclear respiratory factor 2), also named GA-binding protein, is an Ets family transcription factor that controls many genes involved in cell cycle progression and protein synthesis as well as in mitochondrial biogenesis. In this paper, we analyzed the role of NRF-2 in the regulation of human genes involved in mitochondrial DNA transcription and replication. By a combination of bioinformatic and biochemical approaches, we found that the factor binds in vitro and in vivo to the proximal promoter region of the genes coding for the transcription termination factor mTERF, the RNA polymerase POLRMT, the B subunit of the DNA polymerase-gamma, the DNA helicase TWINKLE, and the single-stranded DNA-binding protein mtSSB. The role of NRF-2 in modulating the expression of those genes was further established by RNA interference and overexpression strategies. On the contrary, we found that NRF-2 does not control the genes for the subunit A of DNA polymerase-gamma and for the transcription repressor MTERF3; we suggest that these genes are under regulatory mechanisms that do not involve NRF proteins. Since NRFs are known to positively control the expression of transcription-activating proteins, the novelty emerging from our data is that proteins playing antithetical roles in mitochondrial DNA transcription, namely activators and repressors, are under different regulatory pathways. Finally, we developed a more stringent consensus with respect to the general consensus of NRF-2/GA-binding protein when searching for NRF-2 binding sites in the promoter of mitochondrial proteins.

  3. 76 FR 65190 - Notice of Proposed Information Collection Requests

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-20

    ... Child Left Behind Act of 2001, 4201 et seq., (20 U.S. Code 7171 et seq.), is to provide expanded... Act of 1995 (PRA) (44 U.S.C. 3506(c)(2)(A)), provides the general public and Federal agencies with an... the proposed information collection requests as required by the Paperwork Reduction Act of 1995....

  4. 24 CFR 16.4 - Requests for access; requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Requests for access; requirements. 16.4 Section 16.4 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development IMPLEMENTATION OF THE PRIVACY ACT OF 1974 § 16.4 Requests for access; requirements....

  5. 78 FR 41129 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-09

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Submission for OMB Review; Comment Request Upon Written Request, Copies Available From: Securities..., pursuant to the Paperwork Reduction Act of 1995 (44 U.S.C. 3501 et seq.), the Securities and...

  6. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  7. 16 CFR 1101.22 - Timing: request for time extensions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Timing: request for time extensions. 1101.22 Section 1101.22 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT... Providing Notice and Opportunity To Comment Under Section 6(b)(1) § 1101.22 Timing: request for...

  8. 40 CFR 1601.25 - Disclosure of requested records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Disclosure of requested records. 1601.25 Section 1601.25 Protection of Environment CHEMICAL SAFETY AND HAZARD INVESTIGATION BOARD PROCEDURES FOR DISCLOSURE OF RECORDS UNDER THE FREEDOM OF INFORMATION ACT Procedures for Requesting...

  9. 40 CFR 1601.21 - Responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Responses to requests. 1601.21 Section 1601.21 Protection of Environment CHEMICAL SAFETY AND HAZARD INVESTIGATION BOARD PROCEDURES FOR DISCLOSURE OF RECORDS UNDER THE FREEDOM OF INFORMATION ACT Procedures for Requesting and Disclosing...

  10. 40 CFR 1601.24 - Timing of responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Timing of responses to requests. 1601.24 Section 1601.24 Protection of Environment CHEMICAL SAFETY AND HAZARD INVESTIGATION BOARD PROCEDURES FOR DISCLOSURE OF RECORDS UNDER THE FREEDOM OF INFORMATION ACT Procedures for Requesting...

  11. 40 CFR 1602.2 - Requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Requests for access to records. 1602.2 Section 1602.2 Protection of Environment CHEMICAL SAFETY AND HAZARD INVESTIGATION BOARD PROTECTION OF PRIVACY AND ACCESS TO INDIVIDUAL RECORDS UNDER THE PRIVACY ACT OF 1974 § 1602.2 Requests for access...

  12. 15 CFR 2016.0 - Requests for reviews.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 3 2014-01-01 2014-01-01 false Requests for reviews. 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  13. 15 CFR 2016.0 - Requests for reviews.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 3 2013-01-01 2013-01-01 false Requests for reviews. 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  14. 15 CFR 2016.0 - Requests for reviews

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 3 2011-01-01 2011-01-01 false Requests for reviews 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  15. 75 FR 57961 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-23

    ... HUMAN SERVICES Administration for Children and Families Submission for OMB Review; Comment Request Title: Division of Unaccompanied Children's Services (DUCS) Request for Specific Consent. OMB No.: New Collection. Description: The William Wilberforce Trafficking Victims Protection Reauthorization Act of 2008 (TVPRA of...

  16. 75 FR 81615 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ... HUMAN SERVICES Administration for Children and Families Submission for OMB Review; Comment Request Title: Division of Unaccompanied Children's Services (DUCS) Request for Specific Consent. OMB No.: New Collection. Description: The William Wilberforce Trafficking Victims Protection Reauthorization Act of 2008 (TVPRA of...

  17. 12 CFR 1102.103 - Disclosure of requested records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 9 2012-01-01 2012-01-01 false Disclosure of requested records. 1102.103... the unauthorized disclosure of information pertaining to other individuals. (c) Denial of request for... are not required to be disclosed under provisions of the Freedom of Information Act, 5 U.S.C. 552,...

  18. 10 CFR 780.32 - Response and request for hearing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Response and request for hearing. 780.32 Section 780.32 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.32 Response and request for hearing. Any...

  19. 44 CFR 206.35 - Requests for emergency declarations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Directorate provided that a written request for such extension is made by the Governor, or Acting Governor, during the 30-day period immediately following the incident. The extension request must stipulate the... lives and to protect property, public health and safety, or to lessen or avert the threat of a...

  20. 77 FR 66881 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-07

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Submission for OMB Review; Comment Request Upon Written Request Copies Available From: Securities... given that, pursuant to the Paperwork Reduction Act of 1995 (44 U.S.C. 3501 et. seq.), the...

  1. 5 CFR 1820.4 - Timing of responses to requests.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Timing of responses to requests. 1820.4 Section 1820.4 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS... expedited processing must submit a statement, certified to be true and correct to the best of that...

  2. 32 CFR 701.8 - Processing FOIA requests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... THE NAVY DOCUMENTS AFFECTING THE PUBLIC Department of the Navy Freedom of Information Act (FOIA... signature or a statement certifying under the penalty of perjury that their identity is true and correct... requester shall be made by a statement certified by the requester to be true and correct to the best of...

  3. 40 CFR 2.104 - Responses to requests and appeals.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Procedures for Disclosure of Records Under the Freedom of Information Act § 2.104 Responses to requests and... must submit a statement, certified to be true and correct to the best of your knowledge and belief... determination of your request by an office, you may appeal that determination to the Headquarters Freedom...

  4. 36 CFR 1275.70 - Freedom of information requests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Freedom of information... HISTORICAL MATERIALS OF THE NIXON ADMINISTRATION Access by the Public § 1275.70 Freedom of information requests. (a) The Archivist will process Freedom of Information Act requests for access to only...

  5. 5 CFR 1820.4 - Timing of responses to requests.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Timing of responses to requests. 1820.4 Section 1820.4 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS... expedited processing must submit a statement, certified to be true and correct to the best of that...

  6. 40 CFR 2.104 - Responses to requests and appeals.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Procedures for Disclosure of Records Under the Freedom of Information Act § 2.104 Responses to requests and... must submit a statement, certified to be true and correct to the best of your knowledge and belief... determination of your request by an office, you may appeal that determination to the Headquarters Freedom...

  7. 78 FR 41401 - Proposed Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-10

    ... HUMAN SERVICES Administration for Children and Families Proposed Information Collection Activity; Comment Request Proposed Projects: Title: Tribal Child Support Enforcement Direct Funding Request: 45 CFR... Section 506(c)(2)(A) of the Paperwork Reduction Act of 1995, the Administration for Children and...

  8. 15 CFR 2016.0 - Requests for reviews

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Requests for reviews 2016.0 Section 2016.0 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED... DUTY-FREE TREATMENT UNDER THE ANDEAN TRADE PREFERENCE ACT (ATPA), AS AMENDED § 2016.0 Requests...

  9. 75 FR 22863 - Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ...: In accordance with the Paperwork Reduction Act (44 U.S.C. 3501 et seq.), the Office of Management and... two standard forms: SF-270, Request for Advance or Reimbursement and SF-271, Outlay and Request for... published in the Federal Register and that are open for comment. Simply type ``SF-270 PRA'' (in quotes)...

  10. 76 FR 29263 - Proposed Information Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ... Bureau of Land Management Proposed Information Collection; Comment Request AGENCY: Bureau of Land Management, Interior. ACTION: 60-day notice and request for comments. SUMMARY: In accordance with the Paperwork Reduction Act (PRA), the Bureau of Land Management (BLM) is announcing its intention to...

  11. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  12. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  13. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  14. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air...

  15. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air...

  16. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air...

  17. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air...

  18. 32 CFR 806.8 - Description of requested record.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Description of requested record. 806.8 Section 806.8 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE ADMINISTRATION AIR FORCE FREEDOM OF INFORMATION ACT PROGRAM § 806.8 Description of requested record. Air...

  19. 78 FR 15932 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-13

    ... provisions of the Paperwork Reduction Act (44 U.S.C. Chapter 35). Agency: National Oceanic and Atmospheric...): NA. Type of Request: Regular submission (revision and extension of a current information collection... Hours: 80,637. Needs and Uses: This request is for revision and extension of a current...

  20. 76 FR 69239 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-08

    ... provisions of the Paperwork Reduction Act (44 U.S.C. Chapter 35). Agency: National Oceanic and Atmospheric... Forms 88-164 and 88-166. Type of Request: Regular submission (extension of a current information... minutes. Burden Hours: 1,008. Needs and Uses: This request is for extension of a current...

  1. 10 CFR 1304.105 - Requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Requests for access to records. 1304.105 Section 1304.105 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.105 Requests for access to records.... Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300; Arlington, VA 22201. (c)...

  2. 76 FR 30405 - Information Collection Request Under OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... No: 2011-12939] PEACE CORPS Information Collection Request Under OMB Review AGENCY: Peace Corps. ACTION: 60-Day notice and request for comments. SUMMARY: The Peace Corps will be submitting the following... Reduction Act of 1995 (44 U.S.C. chapter 35), the Peace Corps invites the general public to comment on...

  3. 32 CFR 1665.4 - Requests to amend records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... National Defense Other Regulations Relating to National Defense SELECTIVE SERVICE SYSTEM PRIVACY ACT... records are inaccurate may bring this to the attention of SSS. (b) Procedures. (1)(i) The requests to... request for amendment will include the following: (i) The decision of the Selective Service System...

  4. 45 CFR 508.2 - Request for hearing.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Request for hearing. 508.2 Section 508.2 Public Welfare Regulations Relating to Public Welfare (Continued) FOREIGN CLAIMS SETTLEMENT COMMISSION OF THE... WAR CLAIMS ACT OF 1948, AS AMENDED HEARINGS § 508.2 Request for hearing. Within 30 days after...

  5. 45 CFR 508.2 - Request for hearing.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Request for hearing. 508.2 Section 508.2 Public Welfare Regulations Relating to Public Welfare (Continued) FOREIGN CLAIMS SETTLEMENT COMMISSION OF THE... WAR CLAIMS ACT OF 1948, AS AMENDED HEARINGS § 508.2 Request for hearing. Within 30 days after...

  6. 45 CFR 508.2 - Request for hearing.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Request for hearing. 508.2 Section 508.2 Public Welfare Regulations Relating to Public Welfare (Continued) FOREIGN CLAIMS SETTLEMENT COMMISSION OF THE... WAR CLAIMS ACT OF 1948, AS AMENDED HEARINGS § 508.2 Request for hearing. Within 30 days after...

  7. 45 CFR 508.2 - Request for hearing.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Request for hearing. 508.2 Section 508.2 Public Welfare Regulations Relating to Public Welfare (Continued) FOREIGN CLAIMS SETTLEMENT COMMISSION OF THE... WAR CLAIMS ACT OF 1948, AS AMENDED HEARINGS § 508.2 Request for hearing. Within 30 days after...

  8. 10 CFR 780.32 - Response and request for hearing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Response and request for hearing. 780.32 Section 780.32 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.32 Response and request for hearing. Any...

  9. 10 CFR 780.32 - Response and request for hearing.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Response and request for hearing. 780.32 Section 780.32 Energy DEPARTMENT OF ENERGY PATENT COMPENSATION BOARD REGULATIONS Application for a License Pursuant to Section 153b(2) of the Atomic Energy Act of 1954 § 780.32 Response and request for hearing. Any...

  10. 20 CFR 725.451 - Request for hearing.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Employees' Benefits OFFICE OF WORKERS' COMPENSATION PROGRAMS, DEPARTMENT OF LABOR FEDERAL COAL MINE HEALTH... SAFETY AND HEALTH ACT, AS AMENDED Hearings § 725.451 Request for hearing. After the completion of... request a hearing on any contested issue of fact or law (see § 725.419). A district director may on his...

  11. 7 CFR 1.309 - Answer and request for hearing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Answer and request for hearing. 1.309 Section 1.309 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Procedures Related to Administrative Hearings Under the Program Fraud Civil Remedies Act of 1986 § 1.309 Answer and request for...

  12. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  13. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  14. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  15. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  16. 14 CFR 303.05 - Applications requesting antitrust immunity.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Applications requesting antitrust immunity... Applications requesting antitrust immunity. (a) Each application must state explicitly whether or not the applicant seeks antitrust immunity under the provisions of section 414 of the Act. If antitrust immunity...

  17. 22 CFR 171.32 - Request for access to records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... to ensure a full search, or to ensure that a record retrieved does in fact pertain to the individual... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Request for access to records. 171.32 Section... RECORDS TO THE PUBLIC Privacy Act Provisions § 171.32 Request for access to records. (a) Description...

  18. 5 CFR 9301.9 - Fees for categories of requesters.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act Costs § 9301.9 Fees for categories of... requesters. In responding to commercial use requests, SIGAR shall assess fees that recover the full direct costs of searching for, reviewing and duplicating records. (b) Educational institutions. SIGAR...

  19. 18 CFR 1301.4 - Responsibility for responding to requests.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Responsibility for responding to requests. 1301.4 Section 1301.4 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.4 Responsibility for responding to requests. (a)...

  20. 18 CFR 1301.4 - Responsibility for responding to requests.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Responsibility for responding to requests. 1301.4 Section 1301.4 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.4 Responsibility for responding to requests. (a)...

  1. 18 CFR 1301.4 - Responsibility for responding to requests.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false Responsibility for responding to requests. 1301.4 Section 1301.4 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROCEDURES Freedom of Information Act § 1301.4 Responsibility for responding to requests. (a)...

  2. 75 FR 68631 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-08

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION Submission for OMB Review; Comment Request Upon Written Request, Copies Available From: Securities... to the Paperwork Reduction Act of 1995 (44 U.S.C. 350l-3520), the Securities and Exchange...

  3. 10 CFR 1304.105 - Requests for access to records.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Requests for access to records. 1304.105 Section 1304.105 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.105 Requests for access to records.... Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300; Arlington, VA 22201. (c)...

  4. 10 CFR 1304.105 - Requests for access to records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Requests for access to records. 1304.105 Section 1304.105 Energy NUCLEAR WASTE TECHNICAL REVIEW BOARD PRIVACY ACT OF 1974 § 1304.105 Requests for access to records.... Nuclear Waste Technical Review Board; 2300 Clarendon Blvd., Suite 1300; Arlington, VA 22201. (c)...

  5. 49 CFR 10.31 - Requests for records.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false Requests for records. 10.31 Section 10.31... INDIVIDUALS Availability of Records § 10.31 Requests for records. (a) Ordinarily, each person desiring to... free of charge at http://www.access.gpo.gov/su_docs/aces/PrivacyAct.shtml?desc015.html. See §...

  6. 49 CFR 10.31 - Requests for records.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 1 2014-10-01 2014-10-01 false Requests for records. 10.31 Section 10.31... INDIVIDUALS Availability of Records § 10.31 Requests for records. (a) Ordinarily, each person desiring to... free of charge at http://www.access.gpo.gov/su_docs/aces/PrivacyAct.shtml?desc015.html. See §...

  7. 49 CFR 10.31 - Requests for records.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Requests for records. 10.31 Section 10.31... INDIVIDUALS Availability of Records § 10.31 Requests for records. (a) Ordinarily, each person desiring to... free of charge at http://www.access.gpo.gov/su_docs/aces/PrivacyAct.shtml?desc015.html. See §...

  8. 49 CFR 10.31 - Requests for records.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false Requests for records. 10.31 Section 10.31... INDIVIDUALS Availability of Records § 10.31 Requests for records. (a) Ordinarily, each person desiring to... free of charge at http://www.access.gpo.gov/su_docs/aces/PrivacyAct.shtml?desc015.html. See §...

  9. 49 CFR 10.31 - Requests for records.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false Requests for records. 10.31 Section 10.31... INDIVIDUALS Availability of Records § 10.31 Requests for records. (a) Ordinarily, each person desiring to... free of charge at http://www.access.gpo.gov/su_docs/aces/PrivacyAct.shtml?desc015.html. See §...

  10. 45 CFR 612.5 - Timing of responses to requests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....5 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION... Foundation will make reasonable effort to act on a request within 20 days of when a request is received by... ten working days. (2) Where the extension is for more than ten working days, the FOIA Officer or...

  11. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  12. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 6 2012-07-01 2012-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  13. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  14. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 6 2013-07-01 2013-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  15. 32 CFR 806b.12 - Requesting the Social Security Number.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 6 2011-07-01 2011-07-01 false Requesting the Social Security Number. 806b.12... ADMINISTRATION PRIVACY ACT PROGRAM Collecting Personal Information § 806b.12 Requesting the Social Security Number. When asking an individual for his or her Social Security Number, always give a Privacy...

  16. 22 CFR 171.42 - Requests and identifying information.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Requests and identifying information. 171.42 Section 171.42 Foreign Relations DEPARTMENT OF STATE ACCESS TO INFORMATION AVAILABILITY OF INFORMATION AND RECORDS TO THE PUBLIC Ethics in Government Act Provisions § 171.42 Requests and identifying...

  17. 29 CFR 4220.3 - Requests for PBGC approval.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 9 2013-07-01 2013-07-01 false Requests for PBGC approval. 4220.3 Section 4220.3 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION WITHDRAWAL LIABILITY FOR... representative acting on behalf of a plan sponsor, shall sign and submit the request. (c) Where to file....

  18. 29 CFR 4220.3 - Requests for PBGC approval.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 9 2012-07-01 2012-07-01 false Requests for PBGC approval. 4220.3 Section 4220.3 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION WITHDRAWAL LIABILITY FOR... representative acting on behalf of a plan sponsor, shall sign and submit the request. (c) Where to file....

  19. 78 FR 75576 - Agency Information Collection Activities: Request for Information

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-12

    ...As part of its continuing effort to reduce paperwork and respondent burden, CBP invites the general public and other Federal agencies to comment on an information collection requirement concerning: Request for Information (CBP Form 28). This request for comment is being made pursuant to the Paperwork Reduction Act of 1995 (Pub. L. 104-13; 44 U.S.C....

  20. 77 FR 26051 - Information Collection Request; Submission for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-02

    ...: 2012-10611] PEACE CORPS Information Collection Request; Submission for OMB Review AGENCY: Peace Corps. ACTION: 60-Day notice and request for comments. SUMMARY: The Peace Corps will submit the following... conducting this process in accordance with the Paperwork Reduction Act of 1995 (44 U.S.C. Chapter 35)....

  1. 32 CFR 2402.5 - Responses to requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 6 2014-07-01 2014-07-01 false Responses to requests. 2402.5 Section 2402.5 National Defense Other Regulations Relating to National Defense OFFICE OF SCIENCE AND TECHNOLOGY POLICY REGULATIONS IMPLEMENTING THE FREEDOM OF INFORMATION ACT § 2402.5 Responses to requests. (a) Responses...

  2. 10 CFR 1705.07 - Requests for correction of records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Requests for correction of records. 1705.07 Section 1705.07 Energy DEFENSE NUCLEAR FACILITIES SAFETY BOARD PRIVACY ACT § 1705.07 Requests for correction of..., Defense Nuclear Facilities Safety Board, 625 Indiana Avenue, NW., Suite 700, Washington, DC 20004....

  3. 36 CFR 1007.4 - Preliminary processing of requests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Preliminary processing of... FREEDOM OF INFORMATION ACT § 1007.4 Preliminary processing of requests. (a) Scope of requests. Unless a... containing trade secrets or commercial or financial information submitted by a person outside of the...

  4. 29 CFR 2400.6 - Procedures for requesting records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... REGULATIONS IMPLEMENTING THE PRIVACY ACT § 2400.6 Procedures for requesting records. The purpose of this... effect either in person or by mail to the Privacy Officer, OSHRC, One Lafayette Centre, 1120-20th Street... Privacy Officer shall respond promptly to the request and no later than 10 working days from receipt...

  5. 29 CFR 2400.6 - Procedures for requesting records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... REGULATIONS IMPLEMENTING THE PRIVACY ACT § 2400.6 Procedures for requesting records. The purpose of this... effect either in person or by mail to the Privacy Officer, OSHRC, One Lafayette Centre, 1120-20th Street... Privacy Officer shall respond promptly to the request and no later than 10 working days from receipt...

  6. 29 CFR 2400.6 - Procedures for requesting records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... REGULATIONS IMPLEMENTING THE PRIVACY ACT § 2400.6 Procedures for requesting records. The purpose of this... effect either in person or by mail to the Privacy Officer, OSHRC, One Lafayette Centre, 1120-20th Street... Privacy Officer shall respond promptly to the request and no later than 10 working days from receipt...

  7. 5 CFR 2100.3 - Procedure for requesting information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Procedure for requesting information. 2100.3 Section 2100.3 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.3 Procedure for requesting information. Individuals shall...

  8. 5 CFR 2100.3 - Procedure for requesting information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Procedure for requesting information. 2100.3 Section 2100.3 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.3 Procedure for requesting information. Individuals shall...

  9. 5 CFR 2100.8 - Review of request for amendment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Review of request for amendment. 2100.8 Section 2100.8 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.8 Review of request for amendment. (a) A written acknowledgement of the...

  10. 5 CFR 2100.3 - Procedure for requesting information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Procedure for requesting information. 2100.3 Section 2100.3 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.3 Procedure for requesting information. Individuals shall...

  11. 5 CFR 2100.8 - Review of request for amendment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Review of request for amendment. 2100.8 Section 2100.8 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.8 Review of request for amendment. (a) A written acknowledgement of the...

  12. 5 CFR 2100.3 - Procedure for requesting information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Procedure for requesting information. 2100.3 Section 2100.3 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.3 Procedure for requesting information. Individuals shall...

  13. 5 CFR 2100.8 - Review of request for amendment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Review of request for amendment. 2100.8 Section 2100.8 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.8 Review of request for amendment. (a) A written acknowledgement of the...

  14. 5 CFR 2100.8 - Review of request for amendment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Review of request for amendment. 2100.8 Section 2100.8 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.8 Review of request for amendment. (a) A written acknowledgement of the...

  15. 5 CFR 2100.3 - Procedure for requesting information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Procedure for requesting information. 2100.3 Section 2100.3 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.3 Procedure for requesting information. Individuals shall...

  16. 5 CFR 2100.8 - Review of request for amendment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Review of request for amendment. 2100.8 Section 2100.8 Administrative Personnel ARMED FORCES RETIREMENT HOME ARMED FORCES RETIREMENT HOME PRIVACY ACT PROCEDURES § 2100.8 Review of request for amendment. (a) A written acknowledgement of the...

  17. 36 CFR 902.11 - How records may be requested.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false How records may be requested. 902.11 Section 902.11 Parks, Forests, and Public Property PENNSYLVANIA AVENUE DEVELOPMENT CORPORATION FREEDOM OF INFORMATION ACT General Administration § 902.11 How records may be requested. In...

  18. Mitigating E-Mail Requests in Teenagers' First and Second Language Academic Cyber-Consultation

    ERIC Educational Resources Information Center

    Alcón Soler, Eva

    2013-01-01

    The study analyses teenagers' e-mail requests during academic cyber-consultation, exploring how the performance of request modifiers is influenced by participants' perceptions of the degree of imposition of the speech act and social distance with the recipient. A total of 295 e-mail requests, 145 produced by British English speakers (BES) and 150…

  19. 22 CFR 215.7 - Request for correction or amendment of record.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Request for correction or amendment of record... IMPLEMENTATION OF PRIVACY ACT OF 1974 § 215.7 Request for correction or amendment of record. (a) An individual... correction; and (4) The reasons for the amendment or correction. The request must be accompanied by...

  20. 7 CFR 28.120 - Expenses to be borne by party requesting classification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Expenses to be borne by party requesting... United States Cotton Standards Act Fees and Costs § 28.120 Expenses to be borne by party requesting... shall be borne by the party requesting classification. [56 FR 24673, May 31, 1991]...