Science.gov

Sample records for act sba regulations

  1. 13 CFR 101.109 - Do SBA regulations include the section headings?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Do SBA regulations include the section headings? 101.109 Section 101.109 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.109 Do SBA regulations include the section headings? Yes. All SBA regulations...

  2. 13 CFR 101.108 - Has SBA waived any of the public participation exemptions of the Administrative Procedure Act?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Has SBA waived any of the public participation exemptions of the Administrative Procedure Act? 101.108 Section 101.108 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.108 Has SBA waived any of the...

  3. 13 CFR 107.1630 - SBA regulation of Brokers and Dealers and disclosure to purchasers of Leverage or Trust...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA regulation of Brokers and Dealers and disclosure to purchasers of Leverage or Trust Certificates. 107.1630 Section 107.1630 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES SBA Financial Assistance for Licensees (Leverage)...

  4. 13 CFR 108.1630 - SBA regulation of Brokers and Dealers and disclosure to purchasers of Leverage or Trust...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA regulation of Brokers and Dealers and disclosure to purchasers of Leverage or Trust Certificates. 108.1630 Section 108.1630 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM SBA Financial Assistance for NMVC Companies...

  5. 13 CFR 102.39 - SBA's exempt Privacy Act systems of records.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... in this part promulgated under these provisions: (1) 552a(c)(3) (Accounting of Certain Disclosures...) Investigations Division Management Information System—SBA 17, contains records gathered or created...

  6. 48 CFR 19.810 - SBA appeals.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... intent to appeal. The SBA must send the written appeal to the head of the contracting activity within 15... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false SBA appeals. 19.810....810 SBA appeals. (a) The SBA Administrator may submit the following matters for determination to...

  7. 48 CFR 19.810 - SBA appeals.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... intent to appeal. The SBA must send the written appeal to the head of the contracting activity within 15... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false SBA appeals. 19.810....810 SBA appeals. (a) The SBA Administrator may submit the following matters for determination to...

  8. 13 CFR 120.1600 - General procedures for enforcement actions against SBA Lenders, SBA Supervised Lenders, Other...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... actions against SBA Lenders, SBA Supervised Lenders, Other Regulated SBLCs, Management Officials, Other... procedures for enforcement actions against SBA Lenders, SBA Supervised Lenders, Other Regulated SBLCs... for the enforcement actions listed in paragraphs (b) and (c) of this section, SBA will follow...

  9. 13 CFR 108.1810 - Events of default and SBA's remedies for NMVC Company's noncompliance with terms of Debentures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... substantive regulation promulgated under the Act. (9) Failure to maintain diversity. You fail to maintain diversity between management and ownership as required by § 108.150. (g) SBA remedies for events of...

  10. 13 CFR 108.1810 - Events of default and SBA's remedies for NMVC Company's noncompliance with terms of Debentures.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... substantive regulation promulgated under the Act. (9) Failure to maintain diversity. You fail to maintain diversity between management and ownership as required by § 108.150. (g) SBA remedies for events of...

  11. 75 FR 63703 - Privacy Act of 1974; Privacy Act Regulation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-18

    ...The Board of Governors of the Federal Reserve System (Board) is issuing a final rule to amend its regulation implementing the Privacy Act of 1974 (Privacy Act). The primary changes concern the waiver of copying fees charged to current and former Board employees, and applicants for Board employment, for access to their records under the Privacy Act; the amendment of special procedures for the......

  12. 32 CFR 644.511 - Certificate of competency by SBA.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Certificate of competency by SBA. 644.511 Section... Stone § 644.511 Certificate of competency by SBA. Section 8(b)(7) of the Small Business Act (15 U.S.C. 637(b)(7)) authorizes the SBA to certify the competency of a small business concern as to capacity...

  13. 13 CFR 102.21 - Agency employees responsible for the Privacy Act of 1974.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... systems of records in that office. (c) Senior Agency Official for Privacy is SBA's Chief Information... information privacy protections, including the SBA's full compliance with Federal laws, regulations, and policies relating to information privacy such as the Privacy Act and the E-Government Act of 2002....

  14. 13 CFR 119.17 - What types of oversight will SBA provide to grantees?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... quarterly within 15 calendar days of the end of each quarter. Thereafter, grantees may request that SBA... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What types of oversight will SBA... SBA provide to grantees? (a) In addition to reports required under the Riegle Act, SBA will...

  15. 48 CFR 19.804-3 - SBA acceptance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... C of 13 CFR part 134. ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false SBA acceptance. 19.804-3....804-3 SBA acceptance. (a) Upon receipt of the contracting agency's offer, the SBA will...

  16. 48 CFR 19.804-3 - SBA acceptance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... subpart C of 13 CFR part 134. ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false SBA acceptance. 19.804-3....804-3 SBA acceptance. (a) Upon receipt of the contracting agency's offer, the SBA will...

  17. 13 CFR 121.101 - What are SBA size standards?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are SBA size standards? 121.101 Section 121.101 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Provisions of General Applicability § 121.101 What are SBA size standards? (a) SBA's...

  18. 75 FR 37287 - Privacy Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-29

    ... April 19, 2010 (75 FR 20298) for a public comment period to end on June 18, 2010. This rule amends the... criminal law enforcement duties. (2) From subsection (c)(4) and (d) because notification would alert a... regulations implementing the Privacy Act of 1974 (Privacy Act), Public Law 93-579, 5 U.S.C. 552a. This...

  19. 78 FR 39820 - Notice Seeking Exemption Under the Small Business Investment Act, Conflicts of Interest

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... Act, Conflicts of Interest Notice is hereby given that Salem Halifax Capital Partners, L.P., 2849... Conflicts of Interest of the Small Business Administration (``SBA'') Rules and Regulations (13 CFR 107.730... financing constituting a conflict of interest requiring prior SBA approval. Salem Halifax Capital...

  20. The French Space Operations Act: Technical Regulations

    NASA Astrophysics Data System (ADS)

    Lazare, B.

    2013-12-01

    The French Space Operations Act (FSOA) [1] stipulates that one of the National Technical Regulations' prime objectives is to protect people, property, public health and the environment. Compliance with these Technical Regulations has been mandatory since 10 December, 2010 for space operations by French space operators and for space operations conducted on French territory. The space safety requirements and regulations governing procedures are based on national and international best practices and experience. A critical design review of the space system and procedures shall be carried out by applicant space operators, in order to verify compliance with the Technical Regulations. An independent technical assessment of the operation is delegated to CNES. The principles applied when drafting the Technical Regulations are as follows: requirements must, as far as possible, establish the rules according to the objective to be obtained, rather than how it is to be achieved; requirements must give preference to international standards recognised as being state of the art; requirements must take previous experience into account. The Technical Regulations are divided into three sections covering requirements common to the launch, control and return of a space object. A special section will cover specific rules to be applied at the Guiana Space Centre. The main topics addressed by the Technical Regulations are: operator safety management system; study of risks to people, property, public health and the Earth's environment; impact study on the outer space environment: space debris generated by the operation; planetary protection. The first version of the Technical Regulations [2], issued in March 2011, is dedicated to unmanned space systems.

  1. 48 CFR 619.804-3 - SBA acceptance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false SBA acceptance. 619.804-3 Section 619.804-3 Federal Acquisition Regulations System DEPARTMENT OF STATE SOCIOECONOMIC PROGRAMS SMALL... acceptance....

  2. 48 CFR 619.804-3 - SBA acceptance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false SBA acceptance. 619.804-3 Section 619.804-3 Federal Acquisition Regulations System DEPARTMENT OF STATE SOCIOECONOMIC PROGRAMS SMALL... acceptance....

  3. 48 CFR 1480.102 - Buy Indian Act acquisition regulations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Buy Indian Act acquisition... INDIAN AFFAIRS SUPPLEMENT ACQUISITIONS UNDER THE BUY INDIAN ACT General 1480.102 Buy Indian Act... the Buy Indian Act. (b) This part is under the direct oversight and control of the Chief...

  4. NASA Principal Center for Review of Clean Air Act Regulations

    NASA Technical Reports Server (NTRS)

    Clark-Ingram, Marceia; Munafo, Paul M. (Technical Monitor)

    2002-01-01

    The Clean Air Act (CAA) regulations have greatly impacted materials and processes utilized in the manufacture of aerospace hardware. Code JE/ NASA's Environmental Management Division at NASA Headquarters recognized the need for a formal, Agency-wide review process of CAA regulations. Marshall Space Flight Center (MSFC) was selected as the 'Principal Center for Review of Clean Air Act Regulations'. This presentation describes the centralized support provided by MSFC for the management and leadership of NASA's CAA regulation review process.

  5. 75 FR 35686 - Community Reinvestment Act Regulation Hearings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... TREASURY Office of Thrift Supervision 12 CFR Part 563e Community Reinvestment Act Regulation Hearings... financial institution's performance under the Community Reinvestment Act (CRA). The purpose of the hearings... better serve the goals of the Community Reinvestment Act. The hearings will be held in:...

  6. 48 CFR 1480.102 - Buy Indian Act acquisition regulations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Buy Indian Act acquisition... INDIAN AFFAIRS SUPPLEMENT ACQUISITIONS UNDER THE BUY INDIAN ACT Subpart 1480.1 General 1480.102 Buy... in implementing the Buy Indian Act. (b) This part is under the direct oversight and control of...

  7. 13 CFR 108.30 - Amendments to Act and regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Amendments to Act and regulations. 108.30 Section 108.30 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM Introduction to Part 108 § 108.30 Amendments to Act and regulations. A...

  8. 13 CFR 107.30 - Amendments to Act and regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Amendments to Act and regulations. 107.30 Section 107.30 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Introduction to Part 107 § 107.30 Amendments to Act and regulations. A Licensee shall...

  9. 75 FR 20298 - Privacy Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-19

    ...,'' pursuant to the Privacy Act, as amended (74 FR 60302, Nov. 20, 2009). The Board received no comments on... amendment procedures for this record system. Ivan J. Flores, Paralegal Specialist, Recovery...

  10. 77 FR 75521 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-21

    ... 1, 2005. 70 FR 44256 (Aug. 2, 2005). As explained in the SUPPLEMENTARY INFORMATION section of these... Home Mortgage Disclosure Act (HMDA). 70 FR 12148 (Mar. 11, 2005). See 12 U.S.C. 2808; 12 CFR 203.2(e)(1... the other federal banking agencies in its CRA rule set forth at 12 CFR part 563e. 72 FR 13429 (Mar....

  11. 75 FR 78932 - Federal Seed Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-17

    ...--Zea mays L. subsp. mays'', ``Crambe--Crambe abyssinica R.E. Fr.'', ``Crotalaria, slenderleaf... Federalism implications. Background The FSA, Title II (7 U.S.C. 1571-1575) regulates agricultural...

  12. 78 FR 32595 - Revision of Freedom of Information Act Regulation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ...This proposed rule would amend HUD's regulations implementing the Freedom of Information Act (FOIA). HUD is proposing these amendments to update and streamline HUD's current FOIA regulation. This proposed rule would update HUD's regulations to reflect statutory changes to FOIA, current HUD organizational structure, and current HUD policies and practices with respect to FOIA. Finally, the rule......

  13. 78 FR 79283 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-30

    ... CRA purposes by the OCC, Board, and FDIC on August 2, 2005, effective September 1, 2005. 70 FR 44256... Act (HMDA). 70 FR 12148 (Mar. 11, 2005). See 12 U.S.C. 2808; 12 CFR 203.2(e)(1). On March 22, 2007... other federal banking agencies in its CRA rule previously set forth at 12 CFR 563e. 72 FR 13429 (Mar....

  14. 76 FR 79529 - Community Reinvestment Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... adopted for CRA purposes by the OCC, Board, and FDIC on August 2, 2005, effective September 1, 2005. 70 FR... Disclosure Act (HMDA). 70 FR 12148 (Mar. 22, 2007). See 12 U.S.C. 2808; 12 CFR 203.2(e)(1). On March 22, 2007... federal banking agencies in its CRA rule set forth at 12 CFR 563e. 72 FR 13429. Pursuant to the...

  15. 78 FR 46555 - Privacy Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-01

    ... File ] System--Interior, FWS-20 (48 FR 54719, December 6, 1983); Bureau of Land Management Criminal... proposing to amend its regulations to exempt certain records in the Incident Management, Analysis and... Management, Analysis and Reporting System (IMARS) system of records. IMARS is an incident management...

  16. 75 FR 36270 - Appraisal Subcommittee; Appraiser Regulation; Privacy Act Implementation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-25

    ... From the Federal Register Online via the Government Publishing Office FEDERAL FINANCIAL INSTITUTIONS EXAMINATION COUNCIL 12 CFR Part 1102 Appraisal Subcommittee; Appraiser Regulation; Privacy Act Implementation AGENCY: Appraisal Subcommittee of the Federal Financial Institutions Examination...

  17. 77 FR 56592 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ... Office of the Secretary 43 CFR Part 2 RIN 1093-AA15 Freedom of Information Act Regulations AGENCY: Office... Department follows in processing records under the Freedom of Information Act (``FOIA''). The revisions clarify and update procedures for requesting information from the Department and procedures that...

  18. 78 FR 6216 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... corrections to the final rule published on December 31, 2012 (77 FR 76898). The regulation revises the... publication on December 31, 2012, of the final rule that was the subject of FR Doc. 2012-31117, is corrected... Office of the Secretary 43 CFR Part 2 RIN 1093-AA15 Freedom of Information Act Regulations AGENCY:...

  19. 77 FR 75362 - Government in the Sunshine Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-20

    ... From the Federal Register Online via the Government Publishing Office EQUAL EMPLOYMENT OPPORTUNITY COMMISSION 29 CFR Part 1612 Government in the Sunshine Act Regulations CFR Correction 0 In Title 29 of the Code of Federal Regulations, Parts 900 to 1899, revised as of July 1, 2012, on page 266, in Sec....

  20. 7 CFR 4290.30 - Amendments to Act and regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4290.30 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL BUSINESS INVESTMENT COMPANY (âRBICâ) PROGRAM Introduction to Part 4290 § 4290.30 Amendments to Act and regulations. A RBIC is subject to...

  1. 76 FR 15236 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-21

    ... Information Fee Schedule and Guidelines published by the Office of Management and Budget at 52 FR 10012 (Mar... Information Act (FOIA). The regulations are being revised to update and streamline the language of several... revisions to the Department's regulations under the FOIA to update and streamline the language of...

  2. 75 FR 22320 - Regulations Implementing the Freedom of Information Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-28

    ... HEALTH REVIEW COMMISSION 29 CFR Part 2201 Regulations Implementing the Freedom of Information Act AGENCY: Occupational Safety and Health Review Commission. ACTION: Notice of proposed rulemaking. SUMMARY: The Occupational Safety and Health Review Commission (OSHRC) is proposing to revise its regulations...

  3. 13 CFR 101.107 - What SBA forms are approved for public use?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What SBA forms are approved for public use? 101.107 Section 101.107 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION... Paperwork Reduction Act (44 U.S.C. 3512 and see 5 CFR 1320.6) should notify SBA. Direct such comments to...

  4. 13 CFR 121.411 - What are the size procedures for SBA's Section 8(d) Subcontracting Program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... in the Central Contractor Registration (CCR), or equivalent data base maintained or sanctioned by SBA... SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and...

  5. 75 FR 41370 - Regulations Implementing the Freedom of Information Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ... amended. 75 FR 22320, Apr. 28, 2010. Interested persons were afforded an opportunity to participate in the... HEALTH REVIEW COMMISSION 29 CFR Part 2201 Regulations Implementing the Freedom of Information Act AGENCY: Occupational Safety and Health Review Commission. ACTION: Final rule. SUMMARY: The Occupational Safety...

  6. 76 FR 59071 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... follows: Authority: 50 U.S.C. 401-442; 50 U.S.C. 403a-403v; 5 U.S.C. 552; Executive Order 13392, 70 FR... From the Federal Register Online via the Government Publishing Office CENTRAL INTELLIGENCE AGENCY 32 CFR Part 1900 Freedom of Information Act Regulations AGENCY: Central Intelligence Agency....

  7. 78 FR 50351 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-19

    ... compliance with, among others, the Comprehensive Plan, principals of good planning and urban design, and...; ] NATIONAL CAPITAL PLANNING COMMISSION 1 CFR Part 456 Freedom of Information Act Regulations AGENCY: National Capital Planning Commission. ACTION: Notice of Proposed Rulemaking. SUMMARY: The National Capital...

  8. 76 FR 45403 - Bank Secrecy Act Regulations-Definitions and Other Regulations Relating to Prepaid Access

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-29

    ... regulations. See 75 FR 65806 (October 26, 2010) (Transfer and Reorganization of Bank Secrecy Act Regulations... Relating to Money Services Businesses, 74 FR 22129 (May 12, 2009), was issued before the Chapter X Final...), (c). \\16\\ 75 FR 36589. B. Prior Regulation of Stored Value In 1999, when FinCEN issued its final...

  9. NASA's Principal Center for Review of Clean Air Act Regulations

    NASA Technical Reports Server (NTRS)

    Clark-Ingram, Marceia

    2003-01-01

    Marshall Space Flight Center (MSFC) was selected as the Principal Center for review of Clean Air Act (CAA) regulations. The CAA Principal Center is tasked to: 1) Provide centralized support to NASA/HDQ Code JE for the management and leadership of NASA's CAA regulation review process; 2) Identify potential impact from proposed CAA regulations to NASA program hardware and supporting facilities. The Shuttle Environmental Assurance Initiative, one of the responsibilities of the NASA CAA Working Group (WG), is described in part of this viewgraph presentation.

  10. 13 CFR 120.461 - What are SBA's additional requirements for SBA Supervised Lenders concerning records?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are SBA's additional requirements for SBA Supervised Lenders concerning records? 120.461 Section 120.461 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Lenders Sba Supervised Lenders § 120.461 What are SBA's additional requirements for SBA...

  11. 13 CFR 121.105 - How does SBA define “business concern or concern”?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false How does SBA define âbusiness concern or concernâ? 121.105 Section 121.105 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Provisions of General Applicability § 121.105 How does SBA define...

  12. 13 CFR 121.102 - How does SBA establish size standards?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How does SBA establish size standards? 121.102 Section 121.102 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Provisions of General Applicability § 121.102 How does SBA establish size standards?...

  13. 13 CFR 121.302 - When does SBA determine the size status of an applicant?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false When does SBA determine the size status of an applicant? 121.302 Section 121.302 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Size Eligibility Requirements for Sba Financial Assistance §...

  14. 13 CFR 121.302 - When does SBA determine the size status of an applicant?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false When does SBA determine the size status of an applicant? 121.302 Section 121.302 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Size Eligibility Requirements for Sba Financial Assistance §...

  15. 76 FR 40367 - Federal Acquisition Regulation; Information Collection; Buy American Act-Construction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-08

    ... Regulation; Information Collection; Buy American Act--Construction AGENCY: Department of Defense (DOD... previously approved information collection requirement concerning the Buy American Act--Construction..., American Act--Construction (Grimberg Decision), by any of the following methods: ] Regulations.gov :...

  16. 48 CFR 2419.804-3 - SBA acceptance.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false SBA acceptance. 2419.804-3 Section 2419.804-3 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Small Business Administration Section (8)(a) Program...

  17. Medical loss ratio regulation under the Affordable Care Act.

    PubMed

    Harrington, Scott E

    2013-01-01

    The minimum medical loss ratio (MLR) regulations in the Affordable Care Act guarantee that a specific percentage of health insurance premiums is spent on medical care and specified activities to improve health care quality. This paper analyzes the regulations' potential unintended consequences and incentive effects, including: higher medical costs and premiums for some insurers; less innovation to align consumer, provider, and health plan incentives, less consumer choice and increased market concentration; and the risk that insurers will pay rebates if claim costs are lower than projected when premiums are established, despite the regulations' permitted "credibility adjustments." The paper discusses modifications and alternatives to the MLR regulations to help achieve their stated goals with less potential for adverse effects. PMID:23720876

  18. Balancing act creating the right regulation for coal combustion waste

    SciTech Connect

    Manuel, J.

    2009-11-15

    The December 2008 collapse of a coal ash pond in Tennessee threw safe management of coal combustion waste (CCW) into the spotlight. Millions of tons of CCW are produced in the United States each year, and a large percentage of that is recycled. The US Environmental Protection Agency is pursuing a host of initiatives that could directly or indirectly affect the disposition of CCW. States, too, are taking a look at how they regulate CCW. Among the options is the possibility of regulating CCW under the Resource Conservation and Recovery Act, a move that could have far-reaching implications for both the recycling and the disposal of this waste.

  19. 77 FR 74347 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-14

    ... Information Act (FOIA) (U.S.C. 552) in the Federal Register. See 77 FR 42673, July 20, 2012. CIGIE provided..., Regulatory Planning and Review, 58 FR 51735. This regulation has not been reviewed by the Office of.... Authority: Pub. L. 110-409, 122 Stat. 4302; 5 U.S.C. App; E.O. 12600, 52 FR 23781, 3 CFR, 1987 Comp., p....

  20. 13 CFR 103.2 - Who may conduct business with SBA?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., Participant corporation, or limited liability company, you may conduct business with SBA without a... evidence of his or her authority to act on behalf of an Applicant, Participant, or lender as a condition...

  1. 13 CFR 103.2 - Who may conduct business with SBA?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., Participant corporation, or limited liability company, you may conduct business with SBA without a... evidence of his or her authority to act on behalf of an Applicant, Participant, or lender as a condition...

  2. 76 FR 43585 - Bank Secrecy Act Regulations; Definitions and Other Regulations Relating to Money Services...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-21

    ...The Financial Crimes Enforcement Network (``FinCEN''), a bureau of the Department of the Treasury (``Treasury''), is revising the regulations implementing the Bank Secrecy Act (``BSA'') regarding money services businesses (``MSBs'') to clarify which entities are covered by the definitions. The changes more clearly delineate the scope of entities regulated as MSBs, so that determining which......

  3. 13 CFR 101.101 - Who manages SBA?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Who manages SBA? 101.101 Section 101.101 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.101... appoints employees necessary to implement the Small Business Act, as amended, the Small Business...

  4. 13 CFR 119.12 - What criteria will SBA use to evaluate applications for funding under the PRIME program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... evaluate applications for funding under the PRIME program? 119.12 Section 119.12 Business Credit and...) § 119.12 What criteria will SBA use to evaluate applications for funding under the PRIME program? During... first. SBA will evaluate applications for funding in accordance with the specific goals of the Act,...

  5. 13 CFR 119.12 - What criteria will SBA use to evaluate applications for funding under the PRIME program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... based on expertise and ability to fulfill the purposes of the Act. (2) SBA will evaluate organizational... communication capabilities (or potential for same). SBA will also evaluate data collection capabilities... types of clients served, and the successes realized within the client's organizational goals. (3)...

  6. 13 CFR 120.1010 - SBA access to SBA Lender, Intermediary, and NTAP files.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA access to SBA Lender, Intermediary, and NTAP files. 120.1010 Section 120.1010 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Risk-Based Lender Oversight Supervision § 120.1010 SBA access to SBA...

  7. 13 CFR 120.460 - What are SBA's additional requirements for SBA Supervised Lenders?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are SBA's additional requirements for SBA Supervised Lenders? 120.460 Section 120.460 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Lenders Sba Supervised Lenders § 120.460 What are SBA's additional requirements...

  8. 77 FR 55271 - Truth in Lending Act (Regulation Z); Loan Originator Compensation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-07

    ...The Bureau of Consumer Financial Protection is publishing for public comment a proposed rule amending Regulation Z (Truth in Lending) to implement amendments to the Truth in Lending Act (TILA) made by the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act). The proposal would implement statutory changes made by the Dodd- Frank Act to Regulation Z's current loan......

  9. 7 CFR 800.7 - Information about the Service, Act, and regulations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Information about the Service, Act, and regulations... AGRICULTURE GENERAL REGULATIONS Administration § 800.7 Information about the Service, Act, and regulations. Information about the Grain Inspection, Packers and Stockyards Administration, Service, Act,...

  10. Regulations Under the Americans With Disabilities Act; Genetic Information Nondiscrimination Act. Final rule.

    PubMed

    2016-05-17

    The Equal Employment Opportunity Commission (EEOC or Commission) is issuing its final rule to amend the regulations and interpretive guidance implementing Title I of the Americans with Disabilities Act (ADA) to provide guidance on the extent to which employers may use incentives to encourage employees to participate in wellness programs that ask them to respond to disability-related inquiries and/or undergo medical examinations. This rule applies to all wellness programs that include disability-related inquiries and/or medical examinations whether they are offered only to employees enrolled in an employer-sponsored group health plan, offered to all employees regardless of whether they are enrolled in such a plan, or offered as a benefit of employment by employers that do not sponsor a group health plan or group health insurance. Published elsewhere in this issue of the Federal Register, the EEOC also issued a final rule to amend the regulations implementing Title II of the Genetic Information Nondiscrimination Act (GINA) that addresses the extent to which employers may offer incentives for an employee's spouse to participate in a wellness program. PMID:27192736

  11. 13 CFR 134.807 - SBA response.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... initiative or on motion by a party), the SBA must file any response to the appeal petition no later than 15... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false SBA response. 134.807 Section 134... BEFORE THE OFFICE OF HEARINGS AND APPEALS Rules of Practice for Employee Disputes § 134.807 SBA...

  12. Adaptive Equilibrium Regulation: A Balancing Act in Two Timescales

    PubMed Central

    Boker, Steven M.

    2015-01-01

    An equilibrium involves a balancing of forces. Just as one maintains upright posture in standing or walking, many self-regulatory and interpersonal behaviors can be framed as a balancing act between an ever changing environment and within-person processes. The emerging balance between person and environment, the equilibria, are dynamic and adaptive in response to development and learning. A distinction is made between equilibrium achieved solely due to a short timescale balancing of forces and a longer timescale preferred equilibrium which we define as a state towards which the system slowly adapts. Together, these are developed into a framework that this article calls Adaptive Equilibrium Regulation (ÆR), which separates a regulatory process into two timescales: a faster regulation that automatically balances forces and a slower timescale adaptation process that reconfigures the fast regulation so as to move the system towards its preferred equilibrium when an environmental force persists over the longer timescale. This way of thinking leads to novel models for the interplay between multiple timescales of behavior, learning, and development. PMID:27066197

  13. Polypurine/polypyrimidine sequences as cis-acting transcriptional regulators.

    PubMed

    Brahmachari, S K; Sarkar, P S; Raghavan, S; Narayan, M; Maiti, A K

    1997-04-29

    Genome sequence information has generated increasing evidence for the claim that repetitive DNA sequences present within and around genes could play a important role in the regulation of gene expression. Polypurine/polypyrimidine sequences [poly(Pu/Py)] have been observed in the vicinity of promoters and within the transcribed regions of many genes. To understand whether such sequences influence the level of gene expression, we constructed several prokaryotic and eukaryotic expression vectors incorporating poly(Pu/Py) repeats both within and upstream of a reporter gene, lacZ (encoding beta-galactosidase), and studied its expression in vivo. We find that, in contrast to the situation in Escherichia coli, the presence of poly(Pu/Py) sequences within the gene does not significantly inhibit gene expression in mammalian cells. On the other hand, the presence of such sequences upstream of lacZ leads to a several-fold reduction of gene expression in mammalian cells. Similar down-regulation was observed when a structural cassette containing poly(Pu/Py) sequences upstream of lacZ was integrated into yeast chromosome V. Sequence analysis of the nine totally sequenced yeast chromosomes shows that a large number of such sequences occur upstream of ORFs. On the basis of our experimental results and DNA sequence analysis, we propose that these sequences can function as cis-acting transcriptional regulators. PMID:9185844

  14. 77 FR 43081 - Federal Acquisition Regulation; Information Collection; Buy American Act-Free Trade Agreements...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-23

    ... Regulation; Information Collection; Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate... previously approved information collection requirement concerning the Buy American Act--Free Trade Agreements..., Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate, by any of the...

  15. STENOFOLIA acts as a repressor in regulating leaf blade outgrowth

    PubMed Central

    Lin, Hao; Niu, Lifang; Tadege, Million

    2013-01-01

    We recently reported that the Medicago WOX gene, STENOFOLIA (STF), acts as a transcriptional repressor in regulating leaf blade outgrowth. By using the Nicotiana sylvestris bladeless lam1 mutant as a genetic tool, we showed that the WUS-box, which is conserved among WUS clade WOX genes, is partly responsible for the repressive activity of STF. All members of the modern/WUS clade genes (WUS, WOX1-WOX7) in Arabidopsis that contain intact WUS-box can substitute for STF/LAM1 function while the intermediate and ancient clade members including WOX9,WOX11 and WOX13 cannot, due to lack of the intact WUS-box. Taken together, our results reveal a conserved repression mechanism playing a central role in cell proliferation conferred to the evolutionarily dynamic WOX gene family with acquisition of a repressor domain. PMID:23603965

  16. 7 CFR 4290.507 - Violations based on false filings and nonperformance of agreements with the Secretary or SBA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Violations based on false filings and nonperformance of agreements with the Secretary or SBA. 4290.507 Section 4290.507 Agriculture Regulations of the... with the Secretary or SBA. The following shall constitute a violation of this part: (a)...

  17. 7 CFR 4290.640 - Requirement to file portfolio financing reports with the Secretary (SBA Form 1031).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Requirement to file portfolio financing reports with the Secretary (SBA Form 1031). 4290.640 Section 4290.640 Agriculture Regulations of the Department of... reports with the Secretary (SBA Form 1031). For each Financing you make (excluding guarantees), you...

  18. 7 CFR 4290.640 - Requirement to file portfolio financing reports with the Secretary (SBA Form 1031).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Requirement to file portfolio financing reports with the Secretary (SBA Form 1031). 4290.640 Section 4290.640 Agriculture Regulations of the Department of... reports with the Secretary (SBA Form 1031). For each Financing you make (excluding guarantees), you...

  19. 7 CFR 4290.507 - Violations based on false filings and nonperformance of agreements with the Secretary or SBA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Violations based on false filings and nonperformance of agreements with the Secretary or SBA. 4290.507 Section 4290.507 Agriculture Regulations of the... with the Secretary or SBA. The following shall constitute a violation of this part: (a)...

  20. 78 FR 57444 - Eagle Fund III-A, L.P.; Notice Seeking Exemption Under the Small Business Investment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-18

    ... ADMINISTRATION Eagle Fund III-A, L.P.; Notice Seeking Exemption Under the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Eagle Fund III-A, L.P., 101 S. Hanley Road, Suite 1250, St... Business Administration (``SBA'') Rules and Regulations. Eagle Fund III-A, L.P., provided debt and...

  1. 77 FR 57199 - 2012 Real Estate Settlement Procedures Act (Regulation X) Mortgage Servicing Proposal

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ...The Bureau of Consumer Financial Protection (the Bureau) is proposing to amend Regulation X, which implements the Real Estate Settlement Procedures Act of 1974 (RESPA) and the official interpretation of the regulation. The proposed amendments implement the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act) provisions regarding mortgage loan servicing. Specifically, this......

  2. 78 FR 11279 - Loan Originator Compensation Requirements Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-15

    ...The Bureau of Consumer Financial Protection (Bureau) is amending Regulation Z to implement amendments to the Truth in Lending Act (TILA) made by the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act). The final rule implements requirements and restrictions imposed by the Dodd-Frank Act concerning loan originator compensation; qualifications of, and registration or......

  3. 7 CFR 868.10 - Information about the Service, Act, and regulations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Information about the Service, Act, and regulations. 868.10 Section 868.10 Agriculture Regulations of the Department of Agriculture (Continued) GRAIN... § 868.10 Information about the Service, Act, and regulations. Information about the Service,...

  4. 76 FR 24027 - Federal Acquisition Regulation; Information Collection; Buy American Act Certificate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... Regulation; Information Collection; Buy American Act Certificate AGENCY: Department of Defense (DOD), General... previously approved information collection requirement concerning the Buy American Act certificate. Public... Comment'' screen. Please include your name, company name (if any), and ``Information Collection...

  5. 13 CFR 121.103 - How does SBA determine affiliation?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CFR 2510.3-101(d); (ii) Employee benefit or pension plans established and maintained by the Federal... How does SBA determine affiliation? (a) * * * (7) For SBA's Small Business Innovation Research (SBIR... purposes of SBA's SBIR and STTR programs....

  6. 13 CFR 120.461 - What are SBA's additional requirements for SBA Supervised Lenders concerning records?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What are SBA's additional requirements for SBA Supervised Lenders concerning records? 120.461 Section 120.461 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Lenders Sba Supervised Lenders § 120.461 What are...

  7. 13 CFR 119.10 - Will SBA give preferential consideration to other SBA program participants?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Will SBA give preferential consideration to other SBA program participants? 119.10 Section 119.10 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION PROGRAM FOR INVESTMENT IN MICROENTREPRENEURS (âPRIMEâ OR âTHE ACTâ) § 119.10 Will SBA give preferential consideration...

  8. 13 CFR 119.10 - Will SBA give preferential consideration to other SBA program participants?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Will SBA give preferential consideration to other SBA program participants? 119.10 Section 119.10 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION PROGRAM FOR INVESTMENT IN MICROENTREPRENEURS (âPRIMEâ OR âTHE ACTâ) § 119.10 Will SBA give preferential consideration...

  9. 76 FR 78977 - Real Estate Settlement Procedures Act (Regulation X)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    ... this rule. See 76 FR 43569 (July 21, 2011). \\11\\ Section 1066 of the Dodd-Frank Act grants the...-Frank Act In developing the interim final rule, the Bureau has conducted an analysis of potential... the described analyses and consultations. Although not required by the interim final rule,...

  10. 13 CFR 112.12 - Effect on other regulations; forms and instructions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Effect on other regulations; forms and instructions. 112.12 Section 112.12 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OF SBA-EFFECTUATION OF TITLE VI OF THE CIVIL RIGHTS ACT OF 1964 § 112.12 Effect on...

  11. 60 FR 8812 - Real Estate Settlement Procedures Act (Regulation X); Escrow Accounting Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    1995-02-15

    ... 24 CFR Part 3500 Real Estate Settlement Procedures Act (Regulation X); Escrow Accounting Procedures...-Federal Housing Commissioner 24 CFR Part 3500 RIN 2502-AF77 Real Estate Settlement Procedures Act... of the Real Estate Settlement Procedures Act. Subsequent to the publication of that rule,...

  12. 76 FR 68690 - Rules and Regulations Under the Textile Fiber Products Identification Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-07

    ... Wool Products Labeling Act, and the Fur Products Labeling Act: Final Rule, 63 FR 7508 (Feb. 13, 1998). \\2\\ Federal Trade Commission: Miscellaneous Rules: Final Rule, 63 FR 71582 (Dec. 29, 1998). \\3... and Regulations Under the Wool Products Labeling Act of 1939, Final Rule, 65 FR 75154 (Dec. 1,...

  13. 22 CFR 91.1 - Answering inquiries regarding tariff acts and customs regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Answering inquiries regarding tariff acts and... IMPORT CONTROLS § 91.1 Answering inquiries regarding tariff acts and customs regulations. In replying to inquiries received from exporters, travelers, or other interested parties, concerning tariff acts or...

  14. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Purpose, scope, and applicability of the Job... ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and applicability of the Job Training Partnership Act regulations. (a) Parts...

  15. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Purpose, scope, and applicability of the Job Training Partnership Act regulations. 626.3 Section 626.3 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and...

  16. 20 CFR 626.3 - Purpose, scope, and applicability of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Purpose, scope, and applicability of the Job... ADMINISTRATION, DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.3 Purpose, scope, and applicability of the Job Training Partnership Act regulations. (a) Parts...

  17. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Compliance with the Consumer Credit Protection Act and regulations. 374.3 Section 374.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) SPECIAL REGULATIONS IMPLEMENTATION OF THE CONSUMER CREDIT PROTECTION ACT WITH RESPECT TO AIR CARRIERS...

  18. 29 CFR 1903.2 - Posting of notice; availability of the Act, regulations and applicable standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 5 2010-07-01 2010-07-01 false Posting of notice; availability of the Act, regulations and... § 1903.2 Posting of notice; availability of the Act, regulations and applicable standards. (a)(1) Each... large type, generally not less than 36 pt. (b) Establishment means a single physical location...

  19. 78 FR 29263 - Rules andRegulations Under the Textile Fiber Products Identification Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-20

    ... Under the Textile Fiber Products Identification Act, 76 FR 68690 (Nov. 7, 2011). This Notice of Proposed...--Rules and Regulations Under the Textile Fiber Products Identification Act, 24 FR 4480, 4485 (June 2... Rulemaking, 50 FR 15100 at 15101 (Apr. 15, 1985). This Notice compared the Customs regulations in 19 CFR...

  20. 75 FR 50718 - Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-17

    ...This document contains proposed regulations implementing amendments to the Longshore and Harbor Workers' Compensation Act (LHWCA) by the American Recovery and Reinvestment Act of 2009 (ARRA), relating to the exclusion of certain recreational-vessel workers from the LHWCA's definition of ``employee.'' These regulations would clarify both the definition of ``recreational vessel'' and those......

  1. 77 FR 29982 - Federal Acquisition Regulation; Submission for OMB Review; Davis Bacon Act-Price Adjustment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... (actual method). A notice was published in the Federal Register at 77 FR 13328, on March 6, 2012. No... Regulation; Submission for OMB Review; Davis Bacon Act-Price Adjustment (Actual Method) AGENCY: Department of... Bacon Act-Price Adjustment (Actual Method), by any of the following methods: Regulations.gov :...

  2. 78 FR 4725 - Escrow Requirements Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ...The Bureau of Consumer Financial Protection (Bureau) is publishing a final rule that amends Regulation Z (Truth in Lending) to implement certain amendments to the Truth in Lending Act made by the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act). Regulation Z currently requires creditors to establish escrow accounts for higher-priced mortgage loans secured by a first......

  3. 13 CFR 120.541 - Time for approval by SBA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... approve or deny the request within 15 business days of the date when SBA receives the request. If SBA is unable to approve or deny the request within this 15-day period, SBA will provide a written notice of no... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Time for approval by SBA....

  4. 77 FR 57043 - Regulations Under the Fur Products Labeling Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... Labeling Act (``TFLA''), and, on its own initiative, deleting unnecessary requirements. \\1\\ 76 FR 13550..., most recently in 1967.\\17\\ \\16\\ 15 U.S.C. 69e(b). \\17\\ 32 FR 6023 (Apr. 15, 1967). C. TFLA In 2010....ftc.gov/opa/2011/07/regreview.shtm . \\24\\ 76 FR 13550. \\25\\ The comments, along with a transcript...

  5. 77 FR 66539 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ... electronic records and automated searches along with paper records and manual searches. In addition, FMCS's... twenty cents per page, while document search and review charges will increase to $4.00 per each quarter... revisions read as follows: Sec. 1401.36 Freedom of Information Act fee schedules. (a) * * * (2) Search...

  6. The Equal Access Act: Policy and Regulations for Implementation.

    ERIC Educational Resources Information Center

    Jones, H. Lawrence; Martin, James

    The Equal Access Act of 1984 was drafted to implement access for religious clubs to public secondary schools, but with constitutionally protected safeguards to preclude entanglement of church and state. A number of challenges were made on the constitutional grounds of excessive entanglement of church and state, as well as what the definition of…

  7. 77 FR 50390 - Equal Credit Opportunity Act (Regulation B)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... from the Bureau's rulemaking authority by section 1029 of the Dodd-Frank Act). 76 FR 79442 (Dec. 21... burdens on industry. Thus, many of the rulemakings listed above involve issues that extend across two or... Bureau planned to conduct qualitative testing with industry participants as well as consumers. Each...

  8. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2012-04-01 2011-04-01 true Federal Reserve Regulation Z and Fair Credit Reporting Act... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  9. The Final Regulations to the Carl D. Perkins Vocational and Applied Technology Education Act of 1990.

    ERIC Educational Resources Information Center

    American Vocational Association, Alexandria, VA.

    This publication presents the final regulations to the Carl D. Perkins Vocational and Applied Technology Education Act of 1990. The first section is an analysis of the regulations. This analysis compares the proposed regulations to the final version and explains the differences between the two and the potential impact on program administration.…

  10. 13 CFR 120.462 - What are SBA's additional requirements on capital maintenance for SBA Supervised Lenders?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... until differences between the two calculations are resolved. (e) Capital restoration plan. (1) Filing requirement. An SBA Supervised Lender must file a written capital restoration plan with SBA within 45 days of... Supervised Lender charged with carrying out the capital restoration plan. (3) SBA response. SBA will...

  11. 13 CFR 120.462 - What are SBA's additional requirements on capital maintenance for SBA Supervised Lenders?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... until differences between the two calculations are resolved. (e) Capital restoration plan. (1) Filing requirement. An SBA Supervised Lender must file a written capital restoration plan with SBA within 45 days of... Supervised Lender charged with carrying out the capital restoration plan. (3) SBA response. SBA will...

  12. 13 CFR 120.462 - What are SBA's additional requirements on capital maintenance for SBA Supervised Lenders?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... until differences between the two calculations are resolved. (e) Capital restoration plan. (1) Filing requirement. An SBA Supervised Lender must file a written capital restoration plan with SBA within 45 days of... Supervised Lender charged with carrying out the capital restoration plan. (3) SBA response. SBA will...

  13. 13 CFR 120.462 - What are SBA's additional requirements on capital maintenance for SBA Supervised Lenders?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... until differences between the two calculations are resolved. (e) Capital restoration plan. (1) Filing requirement. An SBA Supervised Lender must file a written capital restoration plan with SBA within 45 days of... Supervised Lender charged with carrying out the capital restoration plan. (3) SBA response. SBA will...

  14. 77 FR 76898 - Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ... Federal Register (77 FR 56592) and requested comments over a 60-day period ending on November 13, 2012... regulations must be based on the best available science and that the rulemaking process must allow for public.... Finally, the revisions include current cost figures to be used in calculating and charging fees...

  15. p23/Sba1p Protects against Hsp90 Inhibitors Independently of Its Intrinsic Chaperone Activity▿ ‡

    PubMed Central

    Forafonov, Fedor; Toogun, Oyetunji A.; Grad, Iwona; Suslova, Elena; Freeman, Brian C.; Picard, Didier

    2008-01-01

    The molecular chaperone Hsp90 assists a subset of cellular proteins and is essential in eukaryotes. A cohort of cochaperones contributes to and regulates the multicomponent Hsp90 machine. Unlike the biochemical activities of the cochaperone p23, its in vivo functions and the structure-function relationship remain poorly understood, even in the genetically tractable model organism Saccharomyces cerevisiae. The SBA1 gene that encodes the p23 ortholog in this species is not an essential gene. We found that in the absence of p23/Sba1p, yeast and mammalian cells are hypersensitive to Hsp90 inhibitors. This protective function of Sba1p depends on its abilities to bind Hsp90 and to block the Hsp90 ATPase and inhibitor binding. In contrast, the protective function of Sba1p does not require the Hsp90-independent molecular chaperone activity of Sba1p. The structure-function analysis suggests that Sba1p undergoes considerable structural rearrangements upon binding Hsp90 and that the large size of the p23/Sba1p-Hsp90 interaction surface facilitates maintenance of high affinity despite sequence divergence during evolution. The large interface may also contribute to preserving a protective function in an environment in which Hsp90 inhibitory compounds can be produced by various microorganisms. PMID:18362168

  16. Auxin acts independently of DELLA proteins in regulating gibberellin levels.

    PubMed

    Reid, James B; Davidson, Sandra E; Ross, John J

    2011-03-01

    Shoot elongation is a vital process for plant development and productivity, in both ecological and economic contexts. Auxin and bioactive gibberellins (GAs), such as GA1, play critical roles in the control of elongation, along with environmental and endogenous factors, including other hormones such as the brassinosteroids. The effect of auxins, such as indole-3-acetic acid (IAA), is at least in part mediated by its effect on GA metabolism, since auxin up-regulates biosynthesis genes such as GA 3-oxidase and GA 20-oxidase and down regulates GA catabolism genes such as GA 2-oxidases, leading to elevated levels of bioactive GA 1. In our recent paper, we have provided evidence that this action of IAA is largely independent of DELLA proteins, the negative regulators of GA action, since the auxin effects are still present in the DELLA-deficient la cry-s genotype of pea. This was a crucial issue to resolve, since like auxin, the DELLAs also promote GA 1 synthesis and inhibit its deactivation. DELLAs are deactivated by GA, and thereby mediate a feedback system by which bioactive GA regulates its own level. However, our recent results, in themselves, do not show the generality of the auxin-GA relationship across species and phylogenetic groups or across different tissue types and responses. Further, they do not touch on the ecological benefits of the auxin-GA interaction. These issues are discussed below as well as the need for the development of suitable experimental systems to allow this process to be examined. PMID:21358281

  17. TRF2 acts as a transcriptional regulator in tumor angiogenesis

    PubMed Central

    Maï, Mounir El; Wagner, Kay-Dietrich; Michiels, Jean-François; Gilson, Eric; Wagner, Nicole

    2015-01-01

    We recently showed that telomeric repeat-binding factor 2 (TRF2) regulates gene expression to promote angiogenesis. We found that TRF2 is highly expressed in tumor vessels and transcriptionally activates platelet-derived growth factor receptor β to promote endothelial cell angiogenic properties independently of its function in telomere protection. This work identifies TRF2 as a promising dual target for cancer therapy. PMID:27308469

  18. Brightening up: the effect of the Physician Payment Sunshine Act on existing regulation of pharmaceutical marketing.

    PubMed

    Gorlach, Igor; Pham-Kanter, Genevieve

    2013-01-01

    With the passage of the Physician Payment Sunshine Act as part of the federal health care reform law, pharmaceutical manufacturers are now required to disclose a wide range of payments made by manufacturers to physicians. We review current state regulation of pharmaceutical marketing and consider how the federal sunshine provision will affect existing marketing regulation. We analyze the legal and practical implications of the Physician Payment Sunshine Act. PMID:23581674

  19. Vapour phase hydrogenation of phenol over rhodium on SBA-15 and SBA-16.

    PubMed

    Giraldo, Liliana; Bastidas-Barranco, Marlon; Moreno-Piraján, Juan Carlos

    2014-01-01

    In the present work, mesoporous SBA-15 and SBA-16 were synthesised using classical methods, and their physicochemical properties were investigated by X-ray diffraction (XRD), FTIR, TEM and N2 adsorption-desorption. Rhodium (Rh, 1 wt %) was loaded on the mesoporous SBA-15 and SBA-16 by an impregnation method. The Rh surface coverage, dispersion and crystallite size were determined by room temperature H2 chemisorption on reduced samples. The catalytic activity of Rh supported on mesoporous SBA-15 and SBA-16 was evaluated for the first time in the hydrogenation of phenol in vapour phase in a temperature range between 130 and 270 °C at atmospheric pressure. The reaction over Rh/SBA-15 at 180 °C produced cyclohexanone as the major product (about 60%) along with lower amounts of cyclohexanol (about 35%) and cyclohexane (about 15%). The influences of temperature, H2/phenol ratio, contact time and the nature of the solvent on the catalytic performance were systematically investigated. The Rh/SBA-16 system offered lower phenol conversion compared to Rh/SBA-15, but both have a very high selectivity for cyclohexanone (above 60%). PMID:25514052

  20. 20 CFR 626.2 - Format of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., including complaint processing and compliance reviews, will be governed by the provisions of 29 CFR part 34... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Format of the Job Training Partnership Act... LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.2 Format of the...

  1. 20 CFR 626.4 - Table of contents for the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the Job Training Partnership Act, 20 CFR parts 626-638 and 1005, 1 is as follows: 1 Part 1005 was... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Table of contents for the Job Training..., DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.4 Table...

  2. 20 CFR 626.2 - Format of the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., including complaint processing and compliance reviews, will be governed by the provisions of 29 CFR part 34... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Format of the Job Training Partnership Act... LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.2 Format of the...

  3. 20 CFR 626.4 - Table of contents for the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the Job Training Partnership Act, 20 CFR parts 626-638 and 1005, 1 is as follows: 1 Part 1005 was... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Table of contents for the Job Training..., DEPARTMENT OF LABOR INTRODUCTION TO THE REGULATIONS UNDER THE JOB TRAINING PARTNERSHIP ACT § 626.4 Table...

  4. The Fair Labor Standards Act and the Child Care Industry--What Regulations Apply.

    ERIC Educational Resources Information Center

    Lee, Elisa

    1996-01-01

    Discusses how the federal Fair Labor Standard Act (FLSA) affects the wages and work hours of child care employees and how FLSA interacts with analogous state laws. Examines how child care centers can determine whether FLSA regulations apply to them, existing exemptions to the current act, minimum wage and overtime, whether training time is working…

  5. 77 FR 13328 - Federal Acquisition Regulation; Information Collection; Davis Bacon Act-Price Adjustment (Actual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... Regulation; Information Collection; Davis Bacon Act--Price Adjustment (Actual Method) AGENCY: Department of... (actual method). Public comments are particularly invited on: Whether this collection of information is... comments identified by Information Collection 9000- 0154, Davis Bacon Act--Price Adjustment (Actual...

  6. 78 FR 39901 - Amendments to the 2013 Mortgage Rules Under the Equal Credit Opportunity Act (Regulation B), Real...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... Bureau issued Escrow Requirements Under the Truth in Lending Act (Regulation Z), 78 FR 4726 (Jan. 30... Settlement Procedures Act (Regulation X), 78 FR 6856 (Jan. 31, 2013) (2013 HOEPA Final Rule), and Ability to Repay and Qualified Mortgage Standards Under the Truth in Lending Act (Regulation Z), 78 FR 6407...

  7. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... credit card practices. (2) The Fair Credit Reporting Act, 15 U.S.C. 1681-1681 setting forth requirements... air carrier and foreign air carrier shall comply with the requirements of Regulation B, 12 CFR part 202, and Regulation Z, 12 CFR part 226, of the Board of Governors of the Federal Reserve Board....

  8. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... credit card practices. (2) The Fair Credit Reporting Act, 15 U.S.C. 1681-1681 setting forth requirements... air carrier and foreign air carrier shall comply with the requirements of Regulation B, 12 CFR part 202, and Regulation Z, 12 CFR part 226, of the Board of Governors of the Federal Reserve Board....

  9. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... credit card practices. (2) The Fair Credit Reporting Act, 15 U.S.C. 1681-1681 setting forth requirements... air carrier and foreign air carrier shall comply with the requirements of Regulation B, 12 CFR part 202, and Regulation Z, 12 CFR part 226, of the Board of Governors of the Federal Reserve Board....

  10. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  11. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  12. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  13. 40 CFR 261.8 - PCB wastes regulated under Toxic Substance Control Act.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false PCB wastes regulated under Toxic... (CONTINUED) SOLID WASTES (CONTINUED) IDENTIFICATION AND LISTING OF HAZARDOUS WASTE General § 261.8 PCB wastes regulated under Toxic Substance Control Act. The disposal of PCB-containing dielectric fluid and...

  14. 75 FR 59609 - Rescission of Regulations Under the Comprehensive Smokeless Tobacco Health Education Act of 1986

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-28

    ... its smokeless tobacco regulations, 16 CFR Part 307, on November 4, 1986.\\1\\ 51 FR 40015. \\1\\ The... on utilitarian items. 56 FR 11654 (Mar. 20, 1991). II. Basis for Removal of Regulations On June 22... the enactment of the Family Smoking Prevention Act. 75 FR 3665 (Jan. 22, ] 2010). The...

  15. 78 FR 8963 - Update of Existing Privacy Act-NASA Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-07

    ... document contains corrections to the final regulations (NASA-2012-0005), which were published in the Federal Register of Thursday, October 4, 2012 (77 FR 60620). The regulations relate to requests to access... SPACE ADMINISTRATION 14 CFR Part 1212 RIN 2700-AD86 Update of Existing Privacy Act--NASA...

  16. GUAM - FACILITIES REGULATED UNDER THE RESOURCE CONVERVATION AND RECOVERY ACT (RCRA)

    EPA Science Inventory

    Points represent facilities that are regulated by the EPA under the Resource Conservation and Recovery Act (RCRA). Facilities regulated under RCRA generate, dispose of, treate or transport hazardous waste. RCRA is a law enacted by Congress in 1976 and amended in 1984 to include ...

  17. NEVADA FACILITIES REGULATED UNDER THE RESOURCE CONVERVATION AND RECOVERY ACT (RCRA)

    EPA Science Inventory

    Points represent facilities that are regulated by the EPA under the Resource Conservation and Recovery Act (RCRA). Facilities regulated under RCRA generate, dispose of, treate or transport hazardous waste. RCRA is a law enacted by Congress in 1976 and amended in 1984 to include ...

  18. 13 CFR 121.105 - How does SBA define “business concern or concern”?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Provisions of General....S.C.A. 1141j) whose size does not exceed the size standard established by SBA for other similar... business concern if a substantial portion of its assets and/or liabilities are the same as those of...

  19. 43 CFR 5441.1-3 - SBA set-aside sales.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false SBA set-aside sales. 5441.1-3 Section 5441.1-3 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR FOREST MANAGEMENT (5000) CONDUCT OF SALES Advertised Sales §...

  20. 43 CFR 5441.1-3 - SBA set-aside sales.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false SBA set-aside sales. 5441.1-3 Section 5441.1-3 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR FOREST MANAGEMENT (5000) CONDUCT OF SALES Advertised Sales §...

  1. 13 CFR 120.1722 - SBA's offset rights.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... offset rights. SBA shall have the right to offset any amount owed by Lender to SBA, including, without limitation, an offset against CSA's obligation to pay Lender pursuant to any Section 504 First Mortgage...

  2. 13 CFR 101.102 - Where is SBA's Headquarters located?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Where is SBA's Headquarters located? 101.102 Section 101.102 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.102 Where is SBA's Headquarters located? The Headquarters of SBA is at 409...

  3. 13 CFR 120.541 - Time for approval by SBA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Time for approval by SBA. 120.541 Section 120.541 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Servicing, Liquidation and Debt Collection Litigation of 7(a) and 504 Loans Sba's Purchase of A Guaranteed Portion § 120.541 Time for approval by SBA....

  4. 13 CFR 120.150 - What are SBA's lending criteria?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false What are SBA's lending criteria? 120.150 Section 120.150 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Policies Applying to All Business Loans Credit Criteria for Sba Loans § 120.150 What are SBA's lending criteria? The applicant (including an...

  5. 13 CFR 120.601 - SBA Secondary Market.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false SBA Secondary Market. 120.601... Market Fiscal and Transfer Agent (fta) § 120.601 SBA Secondary Market. The SBA secondary market (“Secondary Market”) consists of the sale of Certificates, representing either a fractional undivided...

  6. 13 CFR 120.601 - SBA Secondary Market.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA Secondary Market. 120.601... Market Fiscal and Transfer Agent (fta) § 120.601 SBA Secondary Market. The SBA secondary market (“Secondary Market”) consists of the sale of Certificates, representing either the entire guaranteed...

  7. 13 CFR 120.601 - SBA Secondary Market.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false SBA Secondary Market. 120.601... Market Fiscal and Transfer Agent (fta) § 120.601 SBA Secondary Market. The SBA secondary market (“Secondary Market”) consists of the sale of Certificates, representing either the entire guaranteed...

  8. 13 CFR 120.601 - SBA Secondary Market.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false SBA Secondary Market. 120.601... Market Fiscal and Transfer Agent (fta) § 120.601 SBA Secondary Market. The SBA secondary market (“Secondary Market”) consists of the sale of Certificates, representing either a fractional undivided...

  9. 13 CFR 120.601 - SBA Secondary Market.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false SBA Secondary Market. 120.601... Market Fiscal and Transfer Agent (fta) § 120.601 SBA Secondary Market. The SBA secondary market (“Secondary Market”) consists of the sale of Certificates, representing either a fractional undivided...

  10. 13 CFR 101.101 - Who manages SBA?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Who manages SBA? 101.101 Section... Who manages SBA? (a) An Administrator, appointed by the President with the advice and consent of the Senate, manages SBA. The Administrator— (1) Is responsible to the President and Congress for...

  11. 13 CFR 101.101 - Who manages SBA?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Who manages SBA? 101.101 Section... Who manages SBA? (a) An Administrator, appointed by the President with the advice and consent of the Senate, manages SBA. The Administrator— (1) Is responsible to the President and Congress for...

  12. 13 CFR 101.101 - Who manages SBA?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Who manages SBA? 101.101 Section... Who manages SBA? (a) An Administrator, appointed by the President with the advice and consent of the Senate, manages SBA. The Administrator— (1) Is responsible to the President and Congress for...

  13. 13 CFR 101.101 - Who manages SBA?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Who manages SBA? 101.101 Section... Who manages SBA? (a) An Administrator, appointed by the President with the advice and consent of the Senate, manages SBA. The Administrator— (1) Is responsible to the President and Congress for...

  14. 78 FR 18344 - Federal Acquisition Regulation; Submission for OMB Review; Buy American Act-Free Trade Agreements...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-26

    ... Regulation; Submission for OMB Review; Buy American Act--Free Trade Agreements--Israeli Trade Act Certificate... previously approved information collection requirement concerning the Buy American Act--Free Trade Agreements... comments identified by Information Collection 9000- 0130, Buy American Act--Free Trade......

  15. 13 CFR 120.463 - Regulatory accounting-What are SBA's regulatory accounting requirements for SBA Supervised Lenders?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Regulatory accounting-What are SBA's regulatory accounting requirements for SBA Supervised Lenders? 120.463 Section 120.463 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Lenders Sba Supervised Lenders §...

  16. 21 CFR 16.24 - Regulatory hearing required by the act or a regulation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Regulatory hearing required by the act or a regulation. 16.24 Section 16.24 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL REGULATORY HEARING BEFORE THE FOOD AND DRUG ADMINISTRATION Initiation of Proceedings §...

  17. 60 FR 11194 - Real Estate Settlement Procedures Act (Regulation X); Escrow Accounting Procedures

    Federal Register 2010, 2011, 2012, 2013, 2014

    1995-03-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT Office of the Assistant Secretary for Housing-Federal Housing Commissioner 24 CFR Part 3500 RIN 2502-AF77 Real Estate Settlement Procedures Act (Regulation X); Escrow Accounting...

  18. 75 FR 40754 - Government in the Sunshine Act Regulations of the National Science Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-14

    ... From the Federal Register Online via the Government Publishing Office NATIONAL SCIENCE FOUNDATION 45 CFR Part 614 RIN 3145-AA53 Government in the Sunshine Act Regulations of the National Science Board AGENCY: National Science Board (NSB), National Science Foundation (NSF). ACTION: Direct final...

  19. The National Labor Relations Act and the Regulation of Public Employee Collective Bargaining

    ERIC Educational Resources Information Center

    McCann, Walter; Smiley, Stafford

    1976-01-01

    The arguments for and against federal assumption of the responsibility for regulating the relationship between public employers and public employees are analyzed. It is suggested that the National Labor Relations Act should be extended to include them, thereby imposing upon them a duty to bargain collectively. Available from: the Harvard…

  20. 77 FR 20295 - Regulations To Implement the Equal Employment Provisions of the Americans With Disabilities Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-04

    ... From the Federal Register Online via the Government Publishing Office ] EQUAL EMPLOYMENT OPPORTUNITY COMMISSION 29 CFR Part 1630 Regulations To Implement the Equal Employment Provisions of the Americans With Disabilities Act, as Amended CFR Correction In Title 29 of the Code of Federal...

  1. 78 FR 70515 - Petition To Promulgate Standards for Bears Under the Animal Welfare Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-26

    ...; ] DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service 9 CFR Part 3 Petition To Promulgate Standards for Bears Under the Animal Welfare Act Regulations AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Notice of petition. SUMMARY: We are notifying the public that the Animal and...

  2. 75 FR 24862 - Removing Regulations Implementing the Fish and Wildlife Conservation Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-06

    ...'' (59 FR 22951), E.O. 13175, and 512 DM 2. We have determined that there are no potential effects. This... Fish and Wildlife Service 50 CFR Part 83 RIN 1018-AX00 Removing Regulations Implementing the Fish and Wildlife Conservation Act AGENCY: Fish and Wildlife Service, Interior. ACTION: Proposed rule. SUMMARY:...

  3. 75 FR 19241 - Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations; Defining...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... for Mutual Funds, 67 FR 21117 (April 29, 2002); Customer Identification Programs for Mutual Funds, 68 FR 25131 (May 9, 2003); Amendment to the Bank Secrecy Act Regulations--Requirement That Mutual Funds Report Suspicious Activity, 71 FR 26213 (May 4, 2006); Anti-Money Laundering Programs; Special...

  4. 76 FR 57940 - Revision of Department of Justice Freedom of Information Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-19

    ... NRPM published on March 21, 2011 (76 FR 15236), closed on April 20, 2011. This document reopens the... the Freedom of Information Act. The comment period for that rule closed on April 20, 2011. The... revising its existing regulations under the FOIA. (See 76 FR 15236.) The rule proposed to amend...

  5. 76 FR 35963 - Disclosure of Information; Privacy Act Regulations; Notice and Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ... CORPORATION 12 CFR Parts 309 and 310 RIN 3064-AD83 Disclosure of Information; Privacy Act Regulations; Notice... disclosure of information and the PA, as amended. In taking this action the FDIC's goal is to avoid potential... to correct addresses and other matters that have changed since the FDIC's rules involving...

  6. Impact of Clean Air Act Regulations on Nitrogen Fate and Transport in Neuse River Basin

    EPA Science Inventory

    This study investigated impacts of Clean Air Act Amendment (CAAA) NOx emissions regulations on the fate and transport of nitrogen for two watersheds in the Neuse River Basin, North Carolina, USA from 1990 to 2020. The Soil and Water Assessment Tool (SWAT) and the Community Multi-...

  7. 75 FR 14547 - Tribal Consultation on Draft Buy Indian Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Indian Affairs 48 CFR Chapter 14 RIN 1076-AE95 Tribal Consultation on Draft Buy Indian Act Regulations AGENCY: Bureau of Indian Affairs, Interior. ACTION: Notice of tribal consultation...

  8. 14 CFR 374.3 - Compliance with the Consumer Credit Protection Act and regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Compliance with the Consumer Credit... OF TRANSPORTATION (AVIATION PROCEEDINGS) SPECIAL REGULATIONS IMPLEMENTATION OF THE CONSUMER CREDIT PROTECTION ACT WITH RESPECT TO AIR CARRIERS AND FOREIGN AIR CARRIERS § 374.3 Compliance with the...

  9. 76 FR 20569 - Horse Protection Act; Petition for Amendments to Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... Animal and Plant Health Inspection Service 9 CFR Part 11 Horse Protection Act; Petition for Amendments to... changes to our horse protection regulations and our current enforcement practices and related policies... are noting, however, that certain requests in the petition lack authority in the Horse Protection...

  10. Rationing health protection: a proposal to exempt nuisance dust from US Clean Air Act regulations.

    PubMed

    Centner, Terence J; Colson, Gregory

    2013-03-15

    The US House of Representative has passed a bill called the "Farm Dust Regulation Prevention Act" (Dust Act) that would exempt most types of particulate matter (PM) in rural areas from the air quality controls of the US Clean Air Act. The Dust Act would markedly change the country's air quality standards. An examination of the proposed provisions shows that they would exempt non-combustion PM pollutants from mining, smelting, petroleum production, and power generation from existing air quality standards. Persons downwind from pollutants generated in rural areas could be exposed to concentrations of carcinogenic heavy metals, asbestos, and benzene known to adversely affect their health and ecological resources. Existing federal air quality standards based on science would be replaced by a flexible standard that rations health protection. PMID:23376304

  11. Regulating the regulator: Numb acts upstream of p53 to control mammary stem and progenitor cell

    PubMed Central

    Faraldo, Marisa M.

    2015-01-01

    In this issue, Tosoni et al. (2015. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201505037) report that cell fate determinant and tumor suppressor Numb imposes asymmetric cell divisions in mammary stem cells by regulating p53. Numb thereby restricts mammary stem cell expansion and controls the proliferation and lineage-specific characteristics of their progeny. PMID:26598611

  12. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Are my HCT/P's regulated solely under section 361..., AND CELLULAR AND TISSUE-BASED PRODUCTS General Provisions § 1271.10 Are my HCT/P's regulated solely.../P is regulated solely under section 361 of the PHS Act and the regulations in this part if it...

  13. Industry invites regulation: the passage of the Pure Food and Drug Act of 1906.

    PubMed Central

    Barkan, I D

    1985-01-01

    Ending its 27-year stranglehold on proposals for federal pure food and drug legislation, Congress passed the Pure Food and Drug Act and its companion bill, the Meat Inspection Act, on June 30, 1906. An unprecedented convergence of consumer, scientific, and industrial support in 1906 prompted such action; most industries even planned for it, hoping regulation would restore the competitiveness of their products on weak foreign and domestic markets. The ways in which these interests converged, and the reasons therefore, suggest a change in their relationships to each other and with the federal government as America headed into the twentieth century. Images p21-a p21-b PMID:3881052

  14. 13 CFR 107.1230 - Draw-downs by Licensee under SBA's Leverage commitment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Draw-downs by Licensee under SBA's... Commitments by Sba to Reserve Leverage for A Licensee § 107.1230 Draw-downs by Licensee under SBA's Leverage... for a draw against SBA's Leverage commitment, you authorize SBA, or any agent or trustee...

  15. 13 CFR 107.1230 - Draw-downs by Licensee under SBA's Leverage commitment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Draw-downs by Licensee under SBA's... Commitments by Sba to Reserve Leverage for A Licensee § 107.1230 Draw-downs by Licensee under SBA's Leverage... for a draw against SBA's Leverage commitment, you authorize SBA, or any agent or trustee...

  16. 13 CFR 107.1230 - Draw-downs by Licensee under SBA's Leverage commitment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Draw-downs by Licensee under SBA's... Commitments by Sba to Reserve Leverage for A Licensee § 107.1230 Draw-downs by Licensee under SBA's Leverage... for a draw against SBA's Leverage commitment, you authorize SBA, or any agent or trustee...

  17. The Maharashtra Felling of Trees (Regulation) (Amendment) Act, 1988 (No. 26 of 1989), 5 August 1989.

    PubMed

    1989-01-01

    Among other things, this Act amends the Maharashtra (India) Felling of Trees (Regulation) Act, 1964, to authorize the Tree Officer to order the planting of trees in any land (other than government-designated drought land) that he thinks does not contain an adequate number of trees. Owners and occupiers of the land must comply with the order, after being given a reasonable opportunity to be heard. If they do not comply, they can be charged for expenses incurred in having the trees planted. In 1989 the government of West Bengal enacted The Indian Forest (West Bengal Amendment) Act, 1989 (No. 22 of 1988) (Calcutta Gazette, Extraordinary, Part III, 3 February 1989). This Act increases the punishment for various infractions of the Indian Forest Act, 1927, as applied to West Bengal and inserts new language relating to the power of officers to stop and search vehicles; the ability of the state government to seize contraband and related tools, vehicles, boats, and cattle; and punishment for abetting offenses delineated by the Act. PMID:12344326

  18. 13 CFR 119.12 - What criteria will SBA use to evaluate applications for funding under the PRIME program?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... based on expertise and ability to fulfill the purposes of the Act. (2) SBA will evaluate organizational structure, financial stability, financial management systems, personnel capacity, and electronic... types of clients served, and the successes realized within the client's organizational goals. (3)...

  19. 77 FR 75362 - Nondiscrimination in Federally Assisted Programs or Activities of SBA-Effectuation of the Age...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-20

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION 13 CFR Part 117 Nondiscrimination in Federally Assisted Programs or Activities of SBA--Effectuation of the Age Discrimination Act of 1975, as Amended CFR Correction 0 In Title 13 of the Code...

  20. 78 FR 21180 - Announcement of the 2013 SBA-Visa Export Video Contest Under the America COMPETES Reauthorization...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-09

    ... available to support small business exporters. The Cosponsors are looking for creative videos from small... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS... Act of 2011 AGENCY: U.S. Small Business Administration (SBA) ACTION: Notice. SUMMARY: The U.S....

  1. 78 FR 45842 - Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedure Act (Regulation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ... From the Federal Register Online via the Government Publishing Office BUREAU OF CONSUMER FINANCIAL PROTECTION 12 CFR Parts 1024 and 1026 RIN 3170-AA37 Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedure Act (Regulation X) and the Truth in Lending Act (Regulation Z) Correction...

  2. 26 CFR 1.706-2T - Temporary regulations; question and answer under the Tax Reform Act of 1984.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Temporary regulations; question and answer under the Tax Reform Act of 1984. 1.706-2T Section 1.706-2T Internal Revenue INTERNAL REVENUE SERVICE... Partnerships § 1.706-2T Temporary regulations; question and answer under the Tax Reform Act of 1984. Question...

  3. 26 CFR 1.706-2T - Temporary regulations; question and answer under the Tax Reform Act of 1984.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Temporary regulations; question and answer under the Tax Reform Act of 1984. 1.706-2T Section 1.706-2T Internal Revenue INTERNAL REVENUE SERVICE...-2T Temporary regulations; question and answer under the Tax Reform Act of 1984. Question 1:...

  4. 26 CFR 18.0 - Effective date of temporary regulations under the Subchapter S Revision Act of 1982.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 14 2014-04-01 2013-04-01 true Effective date of temporary regulations under the Subchapter S Revision Act of 1982. 18.0 Section 18.0 Internal Revenue INTERNAL REVENUE SERVICE... SUBCHAPTER S REVISION ACT OF 1982 § 18.0 Effective date of temporary regulations under the Subchapter...

  5. 26 CFR 18.0 - Effective date of temporary regulations under the Subchapter S Revision Act of 1982.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 14 2012-04-01 2012-04-01 false Effective date of temporary regulations under the Subchapter S Revision Act of 1982. 18.0 Section 18.0 Internal Revenue INTERNAL REVENUE SERVICE... SUBCHAPTER S REVISION ACT OF 1982 § 18.0 Effective date of temporary regulations under the Subchapter...

  6. HIPAA Mental Health Parity Act--IRS. Notice of proposed rulemaking by cross-reference to temporary regulations.

    PubMed

    1997-12-22

    Elsewhere in this issue of the Federal Register, the IRS is issuing temporary regulations relating to mental health parity requirements imposed on group health plans. These requirements were added to the Internal Revenue Code by section 1532 of the Taxpayer Relief Act of 1997. The IRS is issuing the temporary regulations at the same time that the Pension and Welfare Benefits Administration of the U.S. Department of Labor and the Health Care Financing Administration of the U.S. Department of Health and Human Services are issuing substantially similar interim final regulations relating to mental health parity requirements added by the Mental Health Parity Act of 1996 to the Employee Retirement Income Security Act of 1974 and the Public Health Service Act. The temporary regulations provide guidance to employers and group health plans relating to the new mental health parity requirements. The text of those temporary regulations also serves as the text of these proposed regulations. PMID:10179306

  7. 13 CFR 107.115 - 1940 Act and 1980 Act Companies.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .... 107.115 Section 107.115 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS... Companies. A 1940 Act or 1980 Act Company is eligible to apply for an SBIC license, and an existing Licensee is eligible to apply for SBA's approval to convert to a 1940 Act or 1980 Act Company. In either...

  8. 13 CFR 121.102 - How does SBA establish size standards?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... § 121.102 How does SBA establish size standards? (a) SBA considers economic characteristics comprising... from other industries, growth trends, historical activity within an industry, unique factors...

  9. 78 FR 44685 - Amendments to the 2013 Mortgage Rules Under the Real Estate Settlement Procedures Act (Regulation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-24

    ... Final Rule), 78 FR 6407 (Jan. 30, 2013). \\2\\ Mortgage Servicing Rules Under the Real Estate Settlement... Final Rules), 78 FR 10695 (Feb. 14, 2013) (Regulation X), 78 FR 10901 (Feb. 14, 2013) (Regulation Z). \\3\\ Amendments to the 2013 Escrows Final Rule under the Truth in Lending Act (Regulation Z), 78 FR 30739 (May...

  10. 78 FR 13298 - Notice of Retrospective Review of the Americans With Disabilities Act Regulations for Over-the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-27

    ... Statement in the Federal Register published on April 11, 2000 (65 FR 19477). FOR FURTHER INFORMATION CONTACT... Act Regulations for Over-the-Road Bus Operators; Request for Comments AGENCY: Office of the Secretary...) implementing regulations for over-the-road bus (OTRB) operators. The DOT will review regulations specified...

  11. Revision of Department of Justice Freedom of Information Act and Privacy Act Regulations and Implementation of Electronic Freedom of Information Act Amendments of 1996--DoJ. Proposed Rule.

    PubMed

    1997-08-26

    This document sets forth proposed revision of the Department's regulations under both the Freedom of Information Act (FOIA) and the Privacy Act of 1974. The FOIA and Privacy Act regulations have been streamlined and condensed, in accordance with the principles of the National Performance Reviews. With more "user-friendly" language wherever possible. These revisions also reflect the principles established by President Clinton and Attorney General Reno in their FOIA Policy Memoranda of October 4, 1993. The new statement of discretionary disclosure policy will supercede the existing regulation regarding discretionary access to records of historical interest. Additionally, the regulations have been updated to reflect developments in the case law and to include updated cost figures to be used in calculating and charging fees. These proposed revisions also contain new provisions implementing the Electronic Freedom of Information Act Amendments of 1996. PMID:10169840

  12. Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration

    PubMed Central

    Bernis, Cyril; Swift-Taylor, Beth; Nord, Matthew; Carmona, Sarah; Chook, Yuh Min; Forbes, Douglass J.

    2014-01-01

    The nuclear import receptors importin β and transportin play a different role in mitosis: both act phenotypically as spatial regulators to ensure that mitotic spindle, nuclear membrane, and nuclear pore assembly occur exclusively around chromatin. Importin β is known to act by repressing assembly factors in regions distant from chromatin, whereas RanGTP produced on chromatin frees factors from importin β for localized assembly. The mechanism of transportin regulation was unknown. Diametrically opposed models for transportin action are as follows: 1) indirect action by RanGTP sequestration, thus down-regulating release of assembly factors from importin β, and 2) direct action by transportin binding and inhibiting assembly factors. Experiments in Xenopus assembly extracts with M9M, a superaffinity nuclear localization sequence that displaces cargoes bound by transportin, or TLB, a mutant transportin that can bind cargo and RanGTP simultaneously, support direct inhibition. Consistently, simple addition of M9M to mitotic cytosol induces microtubule aster assembly. ELYS and the nucleoporin 107–160 complex, components of mitotic kinetochores and nuclear pores, are blocked from binding to kinetochores in vitro by transportin, a block reversible by M9M. In vivo, 30% of M9M-transfected cells have spindle/cytokinesis defects. We conclude that the cell contains importin β and transportin “global positioning system”or “GPS” pathways that are mechanistically parallel. PMID:24478460

  13. Carbon material formation on SBA-15 and Ni-SBA-15 and residue constituents during acetylene decomposition.

    PubMed

    Chiang, Hung-Lung; Wu, Trong-Neng; Ho, Yung-Shou; Zeng, Li-Xuan

    2014-07-15

    Carbon materials including carbon spheres and nanotubes were formed from acetylene decomposition on hydrogen-reduced SBA-15 and Ni-SBA-15 at 650-850°C. The physicochemical characteristics of SBA-15, Ni-SBA-15 and carbon materials were analyzed by field emission scanning electronic microscopy (FE-SEM), Raman spectrometry, and energy dispersive spectrometry (EDS). In addition, the contents of polyaromatic hydrocarbons (PAHs) in the tar and residue and volatile organic compounds (VOCs) in the exhaust were determined during acetylene decomposition on SBA-15 and Ni-SBA-15. Spherical carbon materials were observed on SBA-15 during acetylene decomposition at 750 and 850°C. Carbon filaments and ball spheres were formed on Ni-SBA-15 at 650-850°C. Raman spectroscopy revealed peaks at 1290 (D-band, disorder mode, amorphous carbon) and 1590 (G-band, graphite sp(2) structure)cm(-1). Naphthalene (2 rings), pyrene (4 rings), phenanthrene (3 rings), and fluoranthene (4 rings) were major PAHs in tar and residues. Exhaust constituents of hydrocarbon (as propane), H2, and C2H2 were 3.9-2.6/2.7-1.5, 1.4-2.8/2.6-4.3, 4.2-2.4/3.2-1.7% when acetylene was decomposed on SBA-15/Ni-SBA-15, respectively, corresponding to temperatures ranging from 650 to 850°C. The concentrations of 52 VOCs ranged from 9359 to 5658 and 2488 to 1104ppm for SBA-15 and Ni-SBA-15 respectively, at acetylene decomposition temperatures from 650 to 850°C, and the aromatics contributed more than 87% fraction of VOC concentrations. PMID:24858051

  14. The Arabidopsis LYST INTERACTING PROTEIN 5 Acts in Regulating Abscisic Acid Signaling and Drought Response.

    PubMed

    Xia, Zongliang; Huo, Yongjin; Wei, Yangyang; Chen, Qiansi; Xu, Ziwei; Zhang, Wei

    2016-01-01

    Multivesicular bodies (MVBs) are unique endosomes containing vesicles in the lumens and play essential roles in many eukaryotic cellular processes. The Arabidopsis LYST INTERACTING PROTEIN 5 (LIP5), a positive regulator of MVB biogenesis, has critical roles in biotic and abiotic stress responses. However, whether the abscisic acid (ABA) signaling is involved in LIP5-mediated stress response is largely unknown. Here, we report that LIP5 functions in regulating ABA signaling and drought response in Arabidopsis. Analyses of a LIP5 promoter-β-glucuronidase (GUS) construct revealed substantial GUS activity in whole seedlings. The expression of LIP5 was induced by ABA and drought, and overexpression of LIP5 led to ABA hypersensitivity, enhanced stomatal closure, reduced water loss, and, therefore, increased drought tolerance. On the contrary, LIP5 knockdown mutants showed ABA-insensitive phenotypes and reduced drought tolerance; suggesting that LIP5 acts in regulating ABA response. Further analysis using a fluorescent dye revealed that ABA and water stress induced cell endocytosis or vesicle trafficking in a largely LIP5-dependent manner. Furthermore, expression of several drought- or ABA-inducible marker genes was significantly down-regulated in the lip5 mutant seedlings. Collectively, our data suggest that LIP5 positively regulates drought tolerance through ABA-mediated cell signaling. PMID:27313589

  15. The Arabidopsis LYST INTERACTING PROTEIN 5 Acts in Regulating Abscisic Acid Signaling and Drought Response

    PubMed Central

    Xia, Zongliang; Huo, Yongjin; Wei, Yangyang; Chen, Qiansi; Xu, Ziwei; Zhang, Wei

    2016-01-01

    Multivesicular bodies (MVBs) are unique endosomes containing vesicles in the lumens and play essential roles in many eukaryotic cellular processes. The Arabidopsis LYST INTERACTING PROTEIN 5 (LIP5), a positive regulator of MVB biogenesis, has critical roles in biotic and abiotic stress responses. However, whether the abscisic acid (ABA) signaling is involved in LIP5-mediated stress response is largely unknown. Here, we report that LIP5 functions in regulating ABA signaling and drought response in Arabidopsis. Analyses of a LIP5 promoter-β-glucuronidase (GUS) construct revealed substantial GUS activity in whole seedlings. The expression of LIP5 was induced by ABA and drought, and overexpression of LIP5 led to ABA hypersensitivity, enhanced stomatal closure, reduced water loss, and, therefore, increased drought tolerance. On the contrary, LIP5 knockdown mutants showed ABA-insensitive phenotypes and reduced drought tolerance; suggesting that LIP5 acts in regulating ABA response. Further analysis using a fluorescent dye revealed that ABA and water stress induced cell endocytosis or vesicle trafficking in a largely LIP5-dependent manner. Furthermore, expression of several drought- or ABA-inducible marker genes was significantly down-regulated in the lip5 mutant seedlings. Collectively, our data suggest that LIP5 positively regulates drought tolerance through ABA-mediated cell signaling. PMID:27313589

  16. 13 CFR 121.604 - Are 8(a) BD Participants considered small for purposes of other SBA assistance?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Are 8(a) BD Participants considered small for purposes of other SBA assistance? 121.604 Section 121.604 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and...

  17. 13 CFR 121.604 - Are 8(a) BD Participants considered small for purposes of other SBA assistance?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... considered small for purposes of other SBA assistance? 121.604 Section 121.604 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Size Eligibility Requirements for the 8(a) Business Development Program § 121.604 Are 8(a)...

  18. 13 CFR 121.604 - Are 8(a) BD Participants considered small for purposes of other SBA assistance?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... considered small for purposes of other SBA assistance? 121.604 Section 121.604 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Size Eligibility Requirements for the 8(a) Business Development Program § 121.604 Are 8(a)...

  19. 13 CFR 121.604 - Are 8(a) BD Participants considered small for purposes of other SBA assistance?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... considered small for purposes of other SBA assistance? 121.604 Section 121.604 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Size Eligibility Requirements for the 8(a) Business Development Program § 121.604 Are 8(a)...

  20. 13 CFR 121.604 - Are 8(a) BD Participants considered small for purposes of other SBA assistance?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... considered small for purposes of other SBA assistance? 121.604 Section 121.604 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and Standards Size Eligibility Requirements for the 8(a) Business Development Program § 121.604 Are 8(a)...

  1. Identification of trans-acting factors regulating SamDC expression in Oryza sativa

    SciTech Connect

    Basu, Supratim; Roychoudhury, Aryadeep; Sengupta, Dibyendu N.

    2014-03-07

    Highlights: • Identification of cis elements responsible for SamDC expression by in silico analysis. • qPCR analysis of SamDC expression to abiotic and biotic stress treatments. • Detection of SamDC regulators using identified cis-elements as probe by EMSA. • Southwestern Blot analysis to predict the size of the trans-acting factors. - Abstract: Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silico analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the trans-acting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression.

  2. The European "clinical trial" regulation; relationship with the Jardé Act: a Giens workshop.

    PubMed

    Lemaire, François; Marchenay, Brigitte; Chassany, Olivier; Barthélémy, Philippe; Bouzzagou, Mohamed; Comet, Denis; Delval, Cécile; Dubray, Claude; Fouret, Cécile; Frija-Orvoen, Elisabeth; Gambotti, Laetitia; Lamarque, Véronique; d'Orsay, Geneviève; Plattner, Valérie; Sibenaler, Claire; Roux, Jacques; Thoby, Frédérique

    2015-01-01

    In May 2014, the European Union Parliament and Council published a new regulation on clinical trials on medicinal products for human use, which is designed to replace Directive 2001/20/EC. It will not come into effect until 2016. Nevertheless, it is essential to examine its relationship with national legislation, i.e. the Jardé Act, whose implementation has been delayed pending publication of the European regulation. The Giens workshop identified and examined the various issues that this relationship is bound to raise. In particular, it looked at trial methodology assessment procedures, the working relationship between the French National Agency of Drug Safety and Health Products (Agence Nationale de Sécurité du Médicament et des Produits de Santé, ANSM) and ethics committees during the authorization application evaluation phase, review of post-authorization/registration studies on medicinal products and medical devices, and data transparency. PMID:25679191

  3. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly.

    PubMed

    Lettice, Laura A; Horikoshi, Taizo; Heaney, Simon J H; van Baren, Marijke J; van der Linde, Herma C; Breedveld, Guido J; Joosse, Marijke; Akarsu, Nurten; Oostra, Ben A; Endo, Naoto; Shibata, Minoru; Suzuki, Mikio; Takahashi, Eiichi; Shinka, Toshikatsu; Nakahori, Yutaka; Ayusawa, Dai; Nakabayashi, Kazuhiko; Scherer, Stephen W; Heutink, Peter; Hill, Robert E; Noji, Sumihare

    2002-05-28

    Preaxial polydactyly (PPD) is a common limb malformation in human. A number of polydactylous mouse mutants indicate that misexpression of Shh is a common requirement for generating extra digits. Here we identify a translocation breakpoint in a PPD patient and a transgenic insertion site in the polydactylous mouse mutant sasquatch (Ssq). The genetic lesions in both lie within the same respective intron of the LMBR1/Lmbr1 gene, which resides approximately 1 Mb away from Shh. Genetic analysis of Ssq reveals that the Lmbr1 gene is incidental to the phenotype and that the mutation directly interrupts a cis-acting regulator of Shh. This regulator is most likely the target for generating PPD mutations in human. PMID:12032320

  4. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly

    PubMed Central

    Lettice, Laura A.; Horikoshi, Taizo; Heaney, Simon J. H.; van Baren, Marijke J.; van der Linde, Herma C.; Breedveld, Guido J.; Joosse, Marijke; Akarsu, Nurten; Oostra, Ben A.; Endo, Naoto; Shibata, Minoru; Suzuki, Mikio; Takahashi, Eiichi; Shinka, Toshikatsu; Nakahori, Yutaka; Ayusawa, Dai; Nakabayashi, Kazuhiko; Scherer, Stephen W.; Heutink, Peter; Hill, Robert E.; Noji, Sumihare

    2002-01-01

    Preaxial polydactyly (PPD) is a common limb malformation in human. A number of polydactylous mouse mutants indicate that misexpression of Shh is a common requirement for generating extra digits. Here we identify a translocation breakpoint in a PPD patient and a transgenic insertion site in the polydactylous mouse mutant sasquatch (Ssq). The genetic lesions in both lie within the same respective intron of the LMBR1/Lmbr1 gene, which resides ≈1 Mb away from Shh. Genetic analysis of Ssq reveals that the Lmbr1 gene is incidental to the phenotype and that the mutation directly interrupts a cis-acting regulator of Shh. This regulator is most likely the target for generating PPD mutations in human. PMID:12032320

  5. Knowledge of the animal welfare act and animal welfare regulations influences attitudes toward animal research.

    PubMed

    Metzger, Mitchell M

    2015-01-01

    Recent public-opinion polls indicate that Americans have shown a decline in support for animal experimentation, and several reports suggest a relationship between people's knowledge of animal welfare regulations and their attitudes toward animal research. Therefore, this study was designed to assess respondent's knowledge of several provisions in the Animal Welfare Act (AWA) and Animal Welfare Regulations (AWR), and determine whether exposure to elements of this legislation would influence an individual's attitudes toward the use of animals in research. A survey was used to assess knowledge of animal research regulations and attitudes toward animal research from a sample of individuals recruited through Amazon's Mechanical Turk crowdsourcing marketplace. Results from study 1 confirmed the hypothesis that respondents had little knowledge of various federal regulations that govern animal research activities. Data from study 2 revealed that exposure to elements of the AWA and AWR influenced participants' attitudes toward the use of animals in research. These results suggest that providing information to the general public about the AWA and AWR that protect laboratory animals from abuse and neglect may help alleviate concerns about using animals in research settings. PMID:25651094

  6. Knowledge of the Animal Welfare Act and Animal Welfare Regulations Influences Attitudes toward Animal Research

    PubMed Central

    2015-01-01

    Recent public-opinion polls indicate that Americans have shown a decline in support for animal experimentation, and several reports suggest a relationship between people's knowledge of animal welfare regulations and their attitudes toward animal research. Therefore, this study was designed to assess respondent's knowledge of several provisions in the Animal Welfare Act (AWA) and Animal Welfare Regulations (AWR), and determine whether exposure to elements of this legislation would influence an individual's attitudes toward the use of animals in research. A survey was used to assess knowledge of animal research regulations and attitudes toward animal research from a sample of individuals recruited through Amazon's Mechanical Turk crowdsourcing marketplace. Results from study 1 confirmed the hypothesis that respondents had little knowledge of various federal regulations that govern animal research activities. Data from study 2 revealed that exposure to elements of the AWA and AWR influenced participants’ attitudes toward the use of animals in research. These results suggest that providing information to the general public about the AWA and AWR that protect laboratory animals from abuse and neglect may help alleviate concerns about using animals in research settings. PMID:25651094

  7. APP intracellular domain acts as a transcriptional regulator of miR-663 suppressing neuronal differentiation

    PubMed Central

    Shu, R; Wong, W; Ma, Q H; Yang, Z Z; Zhu, H; Liu, F J; Wang, P; Ma, J; Yan, S; Polo, J M; Bernard, C C A; Stanton, L W; Dawe, G S; Xiao, Z C

    2015-01-01

    Amyloid precursor protein (APP) is best known for its involvement in the pathogenesis of Alzheimer's disease. We have previously demonstrated that APP intracellular domain (AICD) regulates neurogenesis; however, the mechanisms underlying AICD-mediated regulation of neuronal differentiation are not yet fully characterized. Using genome-wide chromatin immunoprecipitation approaches, we found that AICD is specifically recruited to the regulatory regions of several microRNA genes, and acts as a transcriptional regulator for miR-663, miR-3648 and miR-3687 in human neural stem cells. Functional assays show that AICD negatively modulates neuronal differentiation through miR-663, a primate-specific microRNA. Microarray data further demonstrate that miR-663 suppresses the expression of multiple genes implicated in neurogenesis, including FBXL18 and CDK6. Our results indicate that AICD has a novel role in suppression of neuronal differentiation via transcriptional regulation of miR-663 in human neural stem cells. PMID:25695604

  8. Identification of trans-acting factors regulating SamDC expression in Oryza sativa.

    PubMed

    Basu, Supratim; Roychoudhury, Aryadeep; Sengupta, Dibyendu N

    2014-03-01

    Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silico analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the trans-acting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression. PMID:24530223

  9. 75 FR 35338 - Implementation of Regulations Required Under Title XI of the Food, Conservation and Energy Act of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-22

    ...-AB07 Implementation of Regulations Required Under Title XI of the Food, Conservation and Energy Act of... participants regarding compliance. In enacting Title XI of the Food, Conservation and Energy Act of 2008 (Farm..., and its coverage was to encompass the complete chain of commerce and give the Secretary of...

  10. 76 FR 39007 - Native American Graves Protection and Repatriation Act Regulations- Definition of “Indian Tribe”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-05

    ... Office of the Secretary of the Interior 43 CFR Part 10 RIN 1024-AD98 Native American Graves Protection... regulations implementing the Native American Graves Protection and Repatriation Act (NAGPRA) removes the... implementation of the Native American Graves Protection and Repatriation Act, including the issuance...

  11. 13 CFR 120.413 - Advertisement of relationship with SBA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Advertisement of relationship with SBA. 120.413 Section 120.413 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Lenders Participation Criteria § 120.413 Advertisement of relationship with SBA. A Lender may refer in its advertising to its participation...

  12. 13 CFR 108.1720 - Characteristics of SBA's guarantee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Characteristics of SBA's guarantee. 108.1720 Section 108.1720 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM SBA Financial Assistance for NMVC Companies (Leverage)...

  13. 13 CFR 120.464 - Reports to SBA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...: (1) Annual Report. Within three months after the close of each fiscal year, each SBA Supervised Lender must submit to SBA two copies of an annual report including audited financial statements as prepared by a certified public accountant in accordance with § 120.463. Specifically, the annual...

  14. 13 CFR 120.464 - Reports to SBA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...: (1) Annual Report. Within three months after the close of each fiscal year, each SBA Supervised Lender must submit to SBA two copies of an annual report including audited financial statements as prepared by a certified public accountant in accordance with § 120.463. Specifically, the annual...

  15. 13 CFR 120.464 - Reports to SBA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...: (1) Annual Report. Within three months after the close of each fiscal year, each SBA Supervised Lender must submit to SBA two copies of an annual report including audited financial statements as prepared by a certified public accountant in accordance with § 120.463. Specifically, the annual...

  16. 77 FR 25779 - SBA Council on Underserved Communities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-01

    ... ADMINISTRATION SBA Council on Underserved Communities AGENCY: U.S. Small Business Administration. ACTION: Notice... location, date, time, and agenda for the first meeting of the SBA Council on Underserved Communities Advisory Council. The meeting will be open to the public. DATES: The meeting will be held on Monday, May...

  17. 13 CFR 120.464 - Reports to SBA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... prepared by a certified public accountant in accordance with § 120.463. Specifically, the annual report... by or against the SBA Supervised Lender, or against any officer, director or employee of the SBA... to be a true, accurate, and complete representation of the financial condition and...

  18. 13 CFR 120.1721 - SBA's right to investigate.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA's right to investigate. 120.1721 Section 120.1721 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS... right to investigate. SBA may undertake such investigation as it deems necessary to determine whether...

  19. 13 CFR 130.810 - SBA review authority.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false SBA review authority. 130.810 Section 130.810 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS DEVELOPMENT CENTERS § 130.810 SBA review authority. (a) Site visits. The AA/SBDCs, or a representative, on notice...

  20. 13 CFR 130.810 - SBA review authority.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA review authority. 130.810 Section 130.810 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS DEVELOPMENT CENTERS § 130.810 SBA review authority. (a) Site visits. The AA/SBDCs, or a representative, on notice...

  1. 13 CFR 130.810 - SBA review authority.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false SBA review authority. 130.810 Section 130.810 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS DEVELOPMENT CENTERS § 130.810 SBA review authority. (a) Site visits. The AA/SBDCs, or a representative, on notice...

  2. 13 CFR 130.810 - SBA review authority.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false SBA review authority. 130.810 Section 130.810 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS DEVELOPMENT CENTERS § 130.810 SBA review authority. (a) Site visits. The AA/SBDCs, or a representative, on notice...

  3. 13 CFR 130.810 - SBA review authority.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false SBA review authority. 130.810 Section 130.810 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS DEVELOPMENT CENTERS § 130.810 SBA review authority. (a) Site visits. The AA/SBDCs, or a representative, on notice...

  4. Tritium removal from tritiated water by organic functionalized SBA-15

    SciTech Connect

    Taguchi, A.; Kato, Y.; Akai, R.; Torikai, Y.; Matsuyama, M.

    2015-03-15

    The recovery of tritium from tritiated water is important for reducing tritium emissions to the environment and for recycling tritium. Meso-porous silicas (SBA-15) were modified by -COOH, -SO{sub 3}H and -NH{sub 2} groups and their tritium adsorption ability from tritiated water under solid-liquid sorption was investigated. The adsorption abilities and separation factor of organic functionalized SBAs were comparable to those of bare SBA. The desorption of water from bare SBA and -COOH functionalized SBA were studied by Fourier transform infra-red spectroscopy using D{sub 2}O as a probe molecule. An interaction was observed for D{sub 2}O with -COOH group where the hydrogen bonds became weaker than D{sub 2}O with bare SBA. (authors)

  5. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Are my HCT/P's regulated solely under section 361... Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS,...

  6. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Are my HCT/P's regulated solely under section 361... Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS,...

  7. MecA Protein Acts as a Negative Regulator of Genetic Competence in Streptococcus mutans

    PubMed Central

    Tian, Xiao-Lin; Dong, Gaofeng; Liu, Tianlei; Gomez, Zubelda A.; Wahl, Astrid; Hols, Pascal

    2013-01-01

    Streptococcus mutans develops competence for genetic transformation through a complex network that receives inputs from at least two signaling peptides, competence-stimulating peptide (CSP) and sigX-inducing peptide (XIP). The key step of competence induction is the transcriptional activation of comX, which encodes an alternative sigma factor, SigX (σX), controlling the expression of late competence genes essential for DNA uptake and recombination. In this study, we provide evidence that MecA acts as a negative regulator in the posttranslational regulation of SigX in S. mutans. Using luxAB transcriptional reporter strains, we demonstrate that MecA represses the expression of late competence genes in S. mutans grown in a complex medium that is subpermissive for competence induction by CSP. The negative regulation of competence by MecA requires the presence of a functional SigX. Accordingly, inactivation of MecA results in a prolonged competence state of S. mutans under this condition. We have also found that the AAA+ protease ClpC displays a similar repressing effect on late competence genes, suggesting that both MecA and ClpC function coordinately to regulate competence in the same regulatory circuit in S. mutans. This suggestion is strongly supported by the results of bacterial two-hybrid assays, which demonstrate that MecA interacts with both SigX and ClpC, forming a ternary SigX-MecA-ClpC complex. Western blot analysis also confirms that inactivation of MecA or ClpC results in the intracellular accumulation of the SigX in S. mutans. Together, our data support the notion that MecA mediates the formation of a ternary SigX-MecA-ClpC complex that sequesters SigX and thereby negatively regulates genetic competence in S. mutans. PMID:24039267

  8. 14-3-3 family members act coordinately to regulate mitotic progression.

    PubMed

    Dalal, Sorab N; Yaffe, Michael B; DeCaprio, James A

    2004-05-01

    The mitosis promoting phosphatase, cdc25C, is a target of both the DNA replication and DNA damage checkpoint pathways. These pathways regulate cdc25C function, in part, by promoting the association of cdc25C with 14-3-3 proteins, which results in the retention of cdc25C in the cytoplasm. To determine which 14-3-3 proteins were required to regulate cdc25C function, we tested the ability of various 14-3-3 family members to form a complex with and negatively regulate cdc25C in human cells. Two 14-3-3 family members, 14-3-3epsilon and 14-3-3gamma specifically formed a complex with cdc25C but not with the 14-3-3 binding defective cdc25C mutant, S216A. In addition, 14-3-3epsilon and 14-3-3gamma inhibited the ability of cdc25C, but not the S216A mutant, to induce premature chromatin condensation (PCC) in U-2OS cells. These results suggested that the reduction in PCC by 14-3-3epsilon and 14-3-3gamma was due to inhibition of cdc25C function. In contrast, 14-3-3sigma was unable to form a complex with cdc25C, but was able to inhibit the ability of both wild type cdc25C and S216A to induce PCC. This suggests that 14-3-3sigma regulates entry into mitosis independently of cdc25C and 14-3-3epsilon and 14-3-3gamma. Thus, specific members of the 14-3-3 family of proteins may act coordinately to maintain the DNA replication checkpoint by regulating the activity of different cell cycle proteins. PMID:15107609

  9. Preparation, characterization, and luminescence of (SBA-15) immobilized pepsin

    NASA Astrophysics Data System (ADS)

    Zhai, Qing-Zhou; Sun, Si-Jia

    2014-12-01

    SBA-15 mesoporous silica was synthesized by hydrothermal method and its surface was methylated by treatment with methyltrimethoxysilane. Pepsin was immobilized on the obtained materials giving host-guest composite materials (SBA-15)-pepsin and (methylated SBA-15)-pepsin. The optimum conditions for preparation of these materials were established. Methylated SBA-15 (M-SBA-15) has improved immobilization efficiency of enzyme compared to initial SBA-15 silica. It was shown that with the gradual increase of NaCl solution ionic strength the immobilized amount of enzyme was reduced. Powder X-ray diffraction and Fourier transform infrared spectroscopy showed that the host frameworks in the prepared host-guest composite materials are intact and the ordered structure was retained. Scanning electron microscopic studies revealed fibrous morphologic characteristics of the SBA-15 and the immobilized pepsin composite materials. The average particle diameter of (SBA-15)-pepsin composite was 338 ± 10 and 343 ± 10 nm for (M-SBA-15)-pepsin. The low temperature N2 adsorption-desorption study at 77 K showed that the pore sizes and specific surface areas of the host-guest composite materials were smaller than those before the introduction of the enzyme, suggesting that the immobilized enzyme occupied a definite position in the host material pore channels. The UV-vis solid diffuse reflectance and luminescence studies showed that the enzyme was successfully immobilized on to the host material and that after the immobilization of enzyme on SBA-15 the conformation of pepsin macromolecule has not been changed.

  10. 13 CFR 124.205 - Can an applicant ask SBA to reconsider SBA's initial decision to decline its application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Can an applicant ask SBA to... DETERMINATIONS 8(a) Business Development Applying to the 8(a) Bd Program § 124.205 Can an applicant ask SBA to... raised in the initial decline, the applicant can ask for reconsideration as if it were an initial decline....

  11. 13 CFR 103.3 - May SBA suspend or revoke an Agent's privilege?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false May SBA suspend or revoke an Agent... STANDARDS FOR CONDUCTING BUSINESS WITH SBA § 103.3 May SBA suspend or revoke an Agent's privilege? The Administrator of SBA or designee may, for good cause, suspend or revoke the privilege of any Agent to...

  12. Eya 1 acts as a critical regulator for specifying the metanephric mesenchyme.

    PubMed

    Sajithlal, Gangadharan; Zou, Dan; Silvius, Derek; Xu, Pin-Xian

    2005-08-15

    Although it is well established that the Gdnf-Ret signal transduction pathway initiates metanephric induction, no single regulator has yet been identified to specify the metanephric mesenchyme or blastema within the intermediate mesoderm, the earliest step of metanephric kidney development and the molecular mechanisms controlling Gdnf expression are essentially unknown. Previous studies have shown that a loss of Eya 1 function leads to renal agenesis that is a likely result of failure of metanephric induction. The studies presented here demonstrate that Eya 1 specifies the metanephric blastema within the intermediate mesoderm at the caudal end of the nephrogenic cord. In contrast to its specific roles in metanephric development, Eya 1 appears dispensable for the formation of nephric duct and mesonephric tubules. Using a combination of null and hypomorphic Eya 1 mutants, we now demonstrated that approximately 20% of normal Eya 1 protein level is sufficient for establishing the metanephric blastema and inducing the ureteric bud formation but not for its normal branching. Using Eya 1, Gdnf, Six 1 and Pax 2 mutant mice, we show that Eya 1 probably functions at the top of the genetic hierarchy controlling kidney organogenesis and it acts in combination with Six 1 and Pax 2 to regulate Gdnf expression during UB outgrowth and branching. These findings uncover an essential function for Eya 1 as a critical determination factor in acquiring metanephric fate within the intermediate mesoderm and as a key regulator of Gdnf expression during ureteric induction and branching morphogenesis. PMID:16018995

  13. Polycomb Group Protein Pcgf6 Acts as a Master Regulator to Maintain Embryonic Stem Cell Identity

    PubMed Central

    Yang, Chao-Shun; Chang, Kung-Yen; Dang, Jason; Rana, Tariq M.

    2016-01-01

    The polycomb repressive complex 1 (PRC1) is a multi-subunit complex that plays critical roles in the epigenetic modulation of gene expression. Here, we show that the PRC1 component polycomb group ring finger 6 (Pcgf6) is required to maintain embryonic stem cell (ESC) identity. In contrast to canonical PRC1, Pcgf6 acts as a positive regulator of transcription and binds predominantly to promoters bearing active chromatin marks. Pcgf6 is expressed at high levels in ESCs, and knockdown reduces the expression of the core ESC regulators Oct4, Sox2, and Nanog. Conversely, Pcgf6 overexpression prevents downregulation of these factors and impairs differentiation. In addition, Pcgf6 enhanced reprogramming in both mouse and human somatic cells. The genomic binding profile of Pcgf6 is highly similar to that of trithorax group proteins, but not of PRC1 or PRC2 complexes, suggesting that Pcgf6 functions atypically in ESCs. Our data reveal novel roles for Pcgf6 in directly regulating Oct4, Nanog, Sox2, and Lin28 expression to maintain ESC identity. PMID:27247273

  14. Epithelial IL-1R2 acts as a homeostatic regulator during remission of ulcerative colitis

    PubMed Central

    Mora-Buch, R; Dotti, I; Planell, N; Calderón-Gómez, E; Jung, P; Masamunt, M C; Llach, J; Ricart, E; Batlle, E; Panés, J; Salas, A

    2016-01-01

    Ulcerative colitis (UC) is a chronic intestinal inflammatory disease that may undergo periods of activity followed by remission. We aimed to identify the endogenous regulatory mechanisms that may promote disease remission. Transcriptional and protein analysis of the intestinal mucosa revealed that the IL-1 decoy receptor, interleukin-1 receptor type 2 (IL1R2), was upregulated in remission compared with active UC and controls. We identified epithelial cells as being responsible for increased IL-1R2 production during remission. Expression of IL1R2 was negatively regulated by Wnt/beta-catenin signals in colonic crypts or epithelial stem cell cultures; accordingly, epithelial stem cells upregulated IL-1R2 upon differentiation. Blocking IL-1R2 in isolated colonic crypt cultures of UC patients in remission and T-cell cultures stimulated with biopsy supernatant from UC patients in remission boosted IL-1β-dependent production of inflammation-related cytokines. Finally, IL1R2 transcription was significantly lower in patients that relapsed during a 1-year follow-up period compared with those in endoscopic remission. Collectively, our results reveal that the IL-1/IL-1R2 axis is differentially regulated in the remitting intestinal mucosa of UC patients. We hypothesize that IL-1R2 in the presence of low concentrations of IL-1β may act locally as a regulator of intestinal homeostasis. PMID:26530134

  15. The NEPA mandate and federal regulation of the natural gas industry. [NEPA (National Environmental Policy Act)

    SciTech Connect

    Hoecker, J.J.

    1992-01-01

    Utility regulators increasingly take responsibility for the [open quotes]extemalities[close quotes] associated with their decisions, meaning the economic and social costs related to rate decisions or other kinds of authorizations. Yet, when Congress adopted the National Environmental Policy Act of 1969 (NEPA), it intervened to ensure protection of the natural environment, not from abuses by the citizenry but from the activities of the federal government itself. Comprised of action forcing procedures, NEPA was designed to infuse the decisional processes of federal agencies with a broad awareness of the environmental consequences of their actions. NEPA encourages decisionmakers to counterbalance the organic statutory and political missions of their departments or agencies with a sensitivity to the ecological consequences of their directives and authorizations. This paper examines how the requirements of NEPA have fared in the environment of classical public utility regulation at the Federal Energy Regulatory Commission. Commission proceedings did not evidence any widely held opinion that economic regulation of the gas industry is hostile to the NEPA process.

  16. 78 FR 30296 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The Commission's regulations include a methodology for oil...

  17. 77 FR 29997 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The Commission's regulations include a methodology for oil...

  18. 76 FR 28966 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods The Commission's regulations include a methodology for oil...

  19. atonal regulates neurite arborization but does not act as a proneural gene in the Drosophila brain

    NASA Technical Reports Server (NTRS)

    Hassan, B. A.; Bermingham, N. A.; He, Y.; Sun, Y.; Jan, Y. N.; Zoghbi, H. Y.; Bellen, H. J.

    2000-01-01

    Drosophila atonal (ato) is the proneural gene of the chordotonal organs (CHOs) in the peripheral nervous system (PNS) and the larval and adult photoreceptor organs. Here, we show that ato is expressed at multiple stages during the development of a lineage of central brain neurons that innervate the optic lobes and are required for eclosion. A novel fate mapping approach shows that ato is expressed in the embryonic precursors of these neurons and that its expression is reactivated in third instar larvae (L3). In contrast to its function in the PNS, ato does not act as a proneural gene in the embryonic brain. Instead, ato performs a novel function, regulating arborization during larval and pupal development by interacting with Notch.

  20. Enhancer of zeste acts as a major developmental regulator of Ciona intestinalis embryogenesis

    PubMed Central

    Le Goff, Emilie; Martinand-Mari, Camille; Martin, Marianne; Feuillard, Jérôme; Boublik, Yvan; Godefroy, Nelly; Mangeat, Paul; Baghdiguian, Stephen; Cavalli, Giacomo

    2015-01-01

    ABSTRACT The paradigm of developmental regulation by Polycomb group (PcG) proteins posits that they maintain silencing outside the spatial expression domains of their target genes, particularly of Hox genes, starting from mid embryogenesis. The Enhancer of zeste [E(z)] PcG protein is the catalytic subunit of the PRC2 complex, which silences its targets via deposition of the H3K27me3 mark. Here, we studied the ascidian Ciona intestinalis counterpart of E(z). Ci-E(z) is detected by immunohistochemistry as soon as the 2- and 4-cell stages as a cytoplasmic form and becomes exclusively nuclear thereafter, whereas the H3K27me3 mark is detected starting from the gastrula stage and later. Morpholino invalidation of Ci-E(z) leads to the total disappearance of both Ci-E(z) protein and its H3K27me3 mark. Ci-E(z) morphants display a severe phenotype. Strikingly, the earliest defects occur at the 4-cell stage with the dysregulation of cell positioning and mitotic impairment. At later stages, Ci-E(z)-deficient embryos are affected by terminal differentiation defects of neural, epidermal and muscle tissues, by the failure to form a notochord and by the absence of caudal nerve. These major phenotypic defects are specifically rescued by injection of a morpholino-resistant Ci-E(z) mRNA, which restores expression of Ci-E(z) protein and re-deposition of the H3K27me3 mark. As observed by qPCR analyses, Ci-E(z) invalidation leads to the early derepression of tissue-specific developmental genes, whereas late-acting developmental genes are generally down-regulated. Altogether, our results suggest that Ci-E(z) plays a major role during embryonic development in Ciona intestinalis by silencing early-acting developmental genes in a Hox-independent manner. PMID:26276097

  1. Aurora-A acts as a tumor suppressor and regulates self-renewal of Drosophila neuroblasts

    PubMed Central

    Wang, Hongyan; Somers, Gregory W.; Bashirullah, Arash; Heberlein, Ulrike; Yu, Fengwei; Chia, William

    2006-01-01

    The choice of self-renewal versus differentiation is a fundamental issue in stem cell and cancer biology. Neural progenitors of the Drosophila post-embryonic brain, larval neuroblasts (NBs), divide asymmetrically in a stem cell-like fashion to generate a self-renewing NB and a Ganglion Mother Cell (GMC), which divides terminally to produce two differentiating neuronal/glial daughters. Here we show that Aurora-A (AurA) acts as a tumor suppressor by suppressing NB self-renewal and promoting neuronal differentiation. In aurA loss-of-function mutants, supernumerary NBs are produced at the expense of neurons. AurA suppresses tumor formation by asymmetrically localizing atypical protein kinase C (aPKC), an NB proliferation factor. Numb, which also acts as a tumor suppressor in larval brains, is a major downstream target of AurA and aPKC. Notch activity is up-regulated in aurA and numb larval brains, and Notch signaling is necessary and sufficient to promote NB self-renewal and suppress differentiation in larval brains. Our data suggest that AurA, aPKC, Numb, and Notch function in a pathway that involved a series of negative genetic interactions. We have identified a novel mechanism for controlling the balance between self-renewal and neuronal differentiation during the asymmetric division of Drosophila larval NBs. PMID:17182870

  2. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis.

    PubMed

    Xu, Jie; Ding, Zhiwen; Vizcay-Barrena, Gema; Shi, Jianxin; Liang, Wanqi; Yuan, Zheng; Werck-Reichhart, Danièle; Schreiber, Lukas; Wilson, Zoe A; Zhang, Dabing

    2014-04-29

    Mature pollen is covered by durable cell walls, principally composed of sporopollenin, an evolutionary conserved, highly resilient, but not fully characterized, biopolymer of aliphatic and aromatic components. Here, we report that ABORTED MICROSPORES (AMS) acts as a master regulator coordinating pollen wall development and sporopollenin biosynthesis in Arabidopsis thaliana. Genome-wide coexpression analysis revealed 98 candidate genes with specific expression in the anther and 70 that showed reduced expression in ams. Among these 70 members, we showed that AMS can directly regulate 23 genes implicated in callose dissociation, fatty acids elongation, formation of phenolic compounds, and lipidic transport putatively involved in sporopollenin precursor synthesis. Consistently, ams mutants showed defective microspore release, a lack of sporopollenin deposition, and a dramatic reduction in total phenolic compounds and cutin monomers. The functional importance of the AMS pathway was further demonstrated by the observation of impaired pollen wall architecture in plant lines with reduced expression of several AMS targets: the abundant pollen coat protein extracellular lipases (EXL5 and EXL6), and CYP98A8 and CYP98A9, which are enzymes required for the production of phenolic precursors. These findings demonstrate the central role of AMS in coordinating sporopollenin biosynthesis and the secretion of materials for pollen wall patterning. PMID:24781116

  3. PKR is activated by cellular dsRNAs during mitosis and acts as a mitotic regulator

    PubMed Central

    Kim, Yoosik; Lee, Jung Hyun; Park, Jong-Eun; Cho, Jun; Yi, Hyerim; Kim, V. Narry

    2014-01-01

    dsRNA-dependent protein kinase R (PKR) is a ubiquitously expressed enzyme well known for its roles in immune response. Upon binding to viral dsRNA, PKR undergoes autophosphorylation, and the phosphorylated PKR (pPKR) regulates translation and multiple signaling pathways in infected cells. Here, we found that PKR is activated in uninfected cells, specifically during mitosis, by binding to dsRNAs formed by inverted Alu repeats (IRAlus). While PKR and IRAlu-containing RNAs are segregated in the cytosol and nucleus of interphase cells, respectively, they interact during mitosis when nuclear structure is disrupted. Once phosphorylated, PKR suppresses global translation by phosphorylating the α subunit of eukaryotic initiation factor 2 (eIF2α). In addition, pPKR acts as an upstream kinase for c-Jun N-terminal kinase and regulates the levels of multiple mitotic factors such as CYCLINS A and B and POLO-LIKE KINASE 1 and phosphorylation of HISTONE H3. Disruption of PKR activation via RNAi or expression of a transdominant-negative mutant leads to misregulation of the mitotic factors, delay in mitotic progression, and defects in cytokinesis. Our study unveils a novel function of PKR and endogenous dsRNAs as signaling molecules during the mitosis of uninfected cells. PMID:24939934

  4. Gamma-aminobutyric acid acts as a specific virulence regulator in Pseudomonas aeruginosa.

    PubMed

    Dagorn, Audrey; Hillion, Mélanie; Chapalain, Annelise; Lesouhaitier, Olivier; Duclairoir Poc, Cécile; Vieillard, Julien; Chevalier, Sylvie; Taupin, Laure; Le Derf, Franck; Feuilloley, Marc G J

    2013-02-01

    Gamma-aminobutyric acid (GABA) is widespread in the environment and can be used by animal and plants as a communication molecule. Pseudomonas species, in particular fluorescent ones, synthesize GABA and express GABA-binding proteins. In this study, we investigated the effects of GABA on the virulence of Pseudomonas aeruginosa. While exposure to GABA (10 µM) did not modify either the growth kinetics or the motility of the bacterium, its cytotoxicity and virulence were strongly increased. The Caenorhabditis elegans 'fast killing test' model revealed that GABA acts essentially through an increase in diffusible toxin(s). GABA also modulates the biofilm formation activity and adhesion properties of PAO1. GABA has no effect on cell surface polarity, biosurfactant secretion or on the lipopolysaccharide structure. The production of several exo-enzymes, pyoverdin and exotoxin A is not modified by GABA but we observed an increase in cyanogenesis which, by itself, could explain the effect of GABA on P. aeruginosa virulence. This mechanism appears to be regulated by quorum sensing. A proteomic analysis revealed that the effect of GABA on cyanogenesis is correlated with a reduction of oxygen accessibility and an over-expression of oxygen-scavenging proteins. GABA also promotes specific changes in the expression of thermostable and unstable elongation factors Tuf/Ts involved in the interaction of the bacterium with the host proteins. Taken together, these results suggest that GABA is a physiological regulator of P. aeruginosa virulence. PMID:23154974

  5. Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis.

    PubMed

    Prévost, Gaëtan; Jeandel, Lydie; Arabo, Arnaud; Coëffier, Moïse; El Ouahli, Mariama; Picot, Marie; Alexandre, David; Gobet, Françoise; Leprince, Jérôme; Berrahmoune, Hind; Déchelotte, Pierre; Malagon, Maria; Bonner, Caroline; Kerr-Conte, Julie; Chigr, Fatiha; Lefebvre, Hervé; Anouar, Youssef; Chartrel, Nicolas

    2015-08-01

    26RFa is a hypothalamic neuropeptide that promotes food intake. 26RFa is upregulated in obese animal models, and its orexigenic activity is accentuated in rodents fed a high-fat diet, suggesting that this neuropeptide might play a role in the development and maintenance of the obese status. As obesity is frequently associated with type 2 diabetes, we investigated whether 26RFa may be involved in the regulation of glucose homeostasis. In the current study, we show a moderate positive correlation between plasma 26RFa levels and plasma insulin in patients with diabetes. Plasma 26RFa concentration also increases in response to an oral glucose tolerance test. In addition, we found that 26RFa and its receptor GPR103 are present in human pancreatic β-cells as well as in the gut. In mice, 26RFa attenuates the hyperglycemia induced by a glucose load, potentiates insulin sensitivity, and increases plasma insulin concentrations. Consistent with these data, 26RFa stimulates insulin production by MIN6 insulinoma cells. Finally, we show, using in vivo and in vitro approaches, that a glucose load induces a massive secretion of 26RFa by the small intestine. Altogether, the present data indicate that 26RFa acts as an incretin to regulate glucose homeostasis. PMID:25858563

  6. Neuropeptide Y acts in the paraventricular nucleus to suppress sympathetic nerve activity and its baroreflex regulation.

    PubMed

    Cassaglia, Priscila A; Shi, Zhigang; Li, Baoxin; Reis, Wagner L; Clute-Reinig, Nicholas M; Stern, Javier E; Brooks, Virginia L

    2014-04-01

    Neuropeptide Y (NPY), a brain neuromodulator that has been strongly implicated in the regulation of energy balance, also acts centrally to inhibit sympathetic nerve activity (SNA); however, the site and mechanism of action are unknown. In chloralose-anaesthetized female rats, nanoinjection of NPY into the paraventricular nucleus of the hypothalamus (PVN) dose-dependently suppressed lumbar SNA (LSNA) and its baroreflex regulation, and these effects were blocked by prior inhibition of NPY Y1 or Y5 receptors. Moreover, PVN injection of Y1 and Y5 receptor antagonists in otherwise untreated rats increased basal and baroreflex control of LSNA, indicating that endogenous NPY tonically inhibits PVN presympathetic neurons. The sympathoexcitation following blockade of PVN NPY inhibition was eliminated by prior PVN nanoinjection of the melanocortin 3/4 receptor inhibitor SHU9119. Moreover, presympathetic neurons, identified immunohistochemically using cholera toxin b neuronal tract tracing from the rostral ventrolateral medulla (RVLM), express NPY Y1 receptor immunoreactivity, and patch-clamp recordings revealed that both NPY and α-melanocyte-stimulating hormone (α-MSH) inhibit and stimulate, respectively, PVN-RVLM neurons. Collectively, these data suggest that PVN NPY inputs converge with α-MSH to influence presympathetic neurons. Together these results identify endogenous NPY as a novel and potent inhibitory neuromodulator within the PVN that may contribute to changes in SNA that occur in states associated with altered energy balance, such as obesity and pregnancy. PMID:24535439

  7. Cis-acting elements in its 3' UTR mediate post-transcriptional regulation of KRAS.

    PubMed

    Kim, Minlee; Kogan, Nicole; Slack, Frank J

    2016-03-15

    Multiple RNA-binding proteins and non-coding RNAs, such as microRNAs (miRNAs), are involved in post-transcriptional gene regulation through recognition motifs in the 3' untranslated region (UTR) of their target genes. The KRAS gene encodes a key signaling protein, and its messenger RNA (mRNA) contains an exceptionally long 3' UTR; this suggests that it may be subject to a highly complex set of regulatory processes. However, 3' UTR-dependent regulation of KRAS expression has not been explored in detail. Using extensive deletion and mutational analyses combined with luciferase reporter assays, we have identified inhibitory and stabilizing cis-acting regions within the KRAS 3' UTR that may interact with miRNAs and RNA-binding proteins, such as HuR. Particularly, we have identified an AU-rich 49-nt fragment in the KRAS 3' UTR that is required for KRAS 3' UTR reporter repression. This element contains a miR-185 complementary element, and we show that overexpression of miR-185 represses endogenous KRAS mRNA and protein in vitro. In addition, we have identified another 49-nt fragment that is required to promote KRAS 3' UTR reporter expression. These findings indicate that multiple cis-regulatory motifs in the 3' UTR of KRAS finely modulate its expression, and sequence alterations within a binding motif may disrupt the precise functions of trans-regulatory factors, potentially leading to aberrant KRAS expression. PMID:26930719

  8. Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis

    NASA Technical Reports Server (NTRS)

    Brown, D. E.; Rashotte, A. M.; Murphy, A. S.; Normanly, J.; Tague, B. W.; Peer, W. A.; Taiz, L.; Muday, G. K.

    2001-01-01

    Polar transport of the plant hormone auxin controls many aspects of plant growth and development. A number of synthetic compounds have been shown to block the process of auxin transport by inhibition of the auxin efflux carrier complex. These synthetic auxin transport inhibitors may act by mimicking endogenous molecules. Flavonoids, a class of secondary plant metabolic compounds, have been suggested to be auxin transport inhibitors based on their in vitro activity. The hypothesis that flavonoids regulate auxin transport in vivo was tested in Arabidopsis by comparing wild-type (WT) and transparent testa (tt4) plants with a mutation in the gene encoding the first enzyme in flavonoid biosynthesis, chalcone synthase. In a comparison between tt4 and WT plants, phenotypic differences were observed, including three times as many secondary inflorescence stems, reduced plant height, decreased stem diameter, and increased secondary root development. Growth of WT Arabidopsis plants on naringenin, a biosynthetic precursor to those flavonoids with auxin transport inhibitor activity in vitro, leads to a reduction in root growth and gravitropism, similar to the effects of synthetic auxin transport inhibitors. Analyses of auxin transport in the inflorescence and hypocotyl of independent tt4 alleles indicate that auxin transport is elevated in plants with a tt4 mutation. In hypocotyls of tt4, this elevated transport is reversed when flavonoids are synthesized by growth of plants on the flavonoid precursor, naringenin. These results are consistent with a role for flavonoids as endogenous regulators of auxin transport.

  9. Neuropeptide Y acts in the paraventricular nucleus to suppress sympathetic nerve activity and its baroreflex regulation

    PubMed Central

    Cassaglia, Priscila A; Shi, Zhigang; Li, Baoxin; Reis, Wagner L; Clute-Reinig, Nicholas M; Stern, Javier E; Brooks, Virginia L

    2014-01-01

    Neuropeptide Y (NPY), a brain neuromodulator that has been strongly implicated in the regulation of energy balance, also acts centrally to inhibit sympathetic nerve activity (SNA); however, the site and mechanism of action are unknown. In chloralose-anaesthetized female rats, nanoinjection of NPY into the paraventricular nucleus of the hypothalamus (PVN) dose-dependently suppressed lumbar SNA (LSNA) and its baroreflex regulation, and these effects were blocked by prior inhibition of NPY Y1 or Y5 receptors. Moreover, PVN injection of Y1 and Y5 receptor antagonists in otherwise untreated rats increased basal and baroreflex control of LSNA, indicating that endogenous NPY tonically inhibits PVN presympathetic neurons. The sympathoexcitation following blockade of PVN NPY inhibition was eliminated by prior PVN nanoinjection of the melanocortin 3/4 receptor inhibitor SHU9119. Moreover, presympathetic neurons, identified immunohistochemically using cholera toxin b neuronal tract tracing from the rostral ventrolateral medulla (RVLM), express NPY Y1 receptor immunoreactivity, and patch-clamp recordings revealed that both NPY and α–melanocyte-stimulating hormone (α-MSH) inhibit and stimulate, respectively, PVN–RVLM neurons. Collectively, these data suggest that PVN NPY inputs converge with α-MSH to influence presympathetic neurons. Together these results identify endogenous NPY as a novel and potent inhibitory neuromodulator within the PVN that may contribute to changes in SNA that occur in states associated with altered energy balance, such as obesity and pregnancy. PMID:24535439

  10. Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis.

    PubMed

    Brown, D E; Rashotte, A M; Murphy, A S; Normanly, J; Tague, B W; Peer, W A; Taiz, L; Muday, G K

    2001-06-01

    Polar transport of the plant hormone auxin controls many aspects of plant growth and development. A number of synthetic compounds have been shown to block the process of auxin transport by inhibition of the auxin efflux carrier complex. These synthetic auxin transport inhibitors may act by mimicking endogenous molecules. Flavonoids, a class of secondary plant metabolic compounds, have been suggested to be auxin transport inhibitors based on their in vitro activity. The hypothesis that flavonoids regulate auxin transport in vivo was tested in Arabidopsis by comparing wild-type (WT) and transparent testa (tt4) plants with a mutation in the gene encoding the first enzyme in flavonoid biosynthesis, chalcone synthase. In a comparison between tt4 and WT plants, phenotypic differences were observed, including three times as many secondary inflorescence stems, reduced plant height, decreased stem diameter, and increased secondary root development. Growth of WT Arabidopsis plants on naringenin, a biosynthetic precursor to those flavonoids with auxin transport inhibitor activity in vitro, leads to a reduction in root growth and gravitropism, similar to the effects of synthetic auxin transport inhibitors. Analyses of auxin transport in the inflorescence and hypocotyl of independent tt4 alleles indicate that auxin transport is elevated in plants with a tt4 mutation. In hypocotyls of tt4, this elevated transport is reversed when flavonoids are synthesized by growth of plants on the flavonoid precursor, naringenin. These results are consistent with a role for flavonoids as endogenous regulators of auxin transport. PMID:11402184

  11. Decree No. 0784 of 18 April 1989 regulating, in part, Act 21 of 1982 and Act 71 of 1988.

    PubMed

    1989-01-01

    This Colombian Decree regulates certain aspects of the system of family allowances. Among other things, it provides that allowances can be granted in terms of food, clothing, school scholarships, textbooks, drugs, and other substitutes for money. Further provisions of the Decree deal with the definition of persons affiliated to the regime, booklets certifying affiliation, family service subsidies, and financing. PMID:12344547

  12. Allowance trading under the Clean Air Act: Who should regulate, and when?

    SciTech Connect

    Lock, R.

    1993-07-01

    The goal of this paper is to explore how compliance with the Clean Air Act Amendments of 1990 (CAAA), especially Title IV and emission trading under it, will affect the current relationship between state and federal regulation. It is difficult, with the limited experience we have had under Title IV, to be definitive about or to be a very strong advocate of too many policy positions. What may be most helpful at this point is to identify where the difficult issues in state/federal relations might arise; and then to explore ways in which tensions might be either avoided or resolved. One anticipated conclusion is that a traditional regulatory mindset could be very destructive if applied to this new area of oversight without due sensitivity to what Congress is trying to achieve in Title IV. That concern pervaded the early legislative debates; and it persists today. Title IV presents some unique challenges to state regulators and will require some creative solutions and fresh thinking if the goals of Congress are to be realized and the full benefits that allowance trading can offer are to be reaped by electricity consumers. In the ultimate analysis, Title IV amounts to a massive internalization of the external costs imposed on society by acid rain deposition. (This places in serious question the notion of additional externality {open_quotes}adders{close_quotes} for sulfur dioxide (SO{sub 2}) and nitrous oxide (NO{sub x}) at the state level for utility supply planning purposes.) The whole point of Title IV is to give those directly charged with compliance, namely power producers, the maximum flexibility to pursue least-cost compliance solutions. Perhaps the biggest single factor in how well they do this will be how state regulators respond to their compliance and allowance trading initiatives.

  13. 77 FR 4498 - Rules and Regulations Under the Wool Products Labeling Act of 1939

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-30

    ... Identification Act, the Wool Products Labeling Act, and the Fur Products Labeling Act: Final Rule, 63 FR 7508...: Miscellaneous Rules: Final Rule, 63 FR 71582 (Dec. 29, 1998). \\7\\ Federal Trade Commission: Rules and... Labeling Act of 1939, Final Rule, 65 FR 75154 (Dec. 1, 2000). \\8\\ The Wool Rules provide that...

  14. Users guide to working in and around water: Regulation under British Columbia`s Water Act. Revised edition

    SciTech Connect

    1998-12-01

    This booklet has been published to help understand and comply with the Regulation under the British Columbia Water Act for works and changes in and about a stream. Works carried out under the Regulation are those that do not involve water diversion, may be completed within a short period of time, and have little environmental impact. Examples include installation or removal of culverts or docks, repair of dikes, beaver dam removal, and installation of drain tile outlets. Information is included on definitions of terms, scope of the Regulation, general conditions for projects, specific project requirements, notification requirements, and enforcement. A copy of the Regulation is included.

  15. The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling1

    PubMed Central

    Wu, Ling; Wang, Chenhui; Boisson, Bertrand; Misra, Saurav; Rayman, Patricia; Finke, James H.; Puel, Anne; Casanova, Jean-Laurent; Li, Xiaoxia

    2014-01-01

    IL-17 is a pro-inflammatory cytokine implicated in the pathogenesis of autoimmune diseases including psoriasis. ACT1 is an essential adaptor molecule in the IL-17 signaling pathway. A missense single nucleotide polymorphism (rs33980500; SNP-D10N) that resulted in the substitution of an asparagine for an aspartic acid at position 10 of ACT1 (ACT1-D10N) is associated with psoriasis susceptibility. Due to alternative splicing in humans, SNP-D10N encodes two mutated ACT1 proteins, ACT1-D10N and ACT1-D19N. Though both ACT1 isoforms are Hsp90 ‘client’ proteins, the nine additional amino acids in ACT1-D19N provide an additional Hsp90 binding site that is absent in ACT1-D10N. Therefore, while ACT1-D10N is a dead protein that is unable to transduce IL-17 signals for gene expression, ACT1-D19N is fully responsive to IL-17. Intriguingly, the two ACT1 isoforms are differentially expressed in ACT1D10N/D10N fibroblasts and T cells. Fibroblasts express both isoforms equally, enabling ACT1-D19N to compensate for the loss of ACT1-D10N function. ACT1D10N/D10N T cells, however, express predominantly ACT1-D10N. Lacking this compensatory mechanism, ACT1D10N/D10N T cells behave like ACT1-deficient T cells, exhibiting a dysregulated and hyperactive Th17 phenotype with overproduction of IL-22 and IL-17. The hyperactive Th17 response combined with fully responsive fibroblasts likely synergized to contribute to psoriasis susceptibility in SNP-D10N patients. PMID:25024377

  16. The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling.

    PubMed

    Wu, Ling; Wang, Chenhui; Boisson, Bertrand; Misra, Saurav; Rayman, Patricia; Finke, James H; Puel, Anne; Casanova, Jean-Laurent; Li, Xiaoxia

    2014-08-15

    IL-17 is a proinflammatory cytokine implicated in the pathogenesis of autoimmune diseases including psoriasis. ACT1 is an essential adaptor molecule in the IL-17 signaling pathway. A missense single nucleotide polymorphism (rs33980500; SNP-D10N) that resulted in the substitution of an asparagine for an aspartic acid at position 10 of ACT1 (ACT1-D10N) is associated with psoriasis susceptibility. Due to alternative splicing in humans, SNP-D10N encodes two mutated ACT1 proteins, ACT1-D10N and ACT1-D19N. Although both ACT1 isoforms are Hsp90 client proteins, the nine additional amino acids in ACT1-D19N provide an additional Hsp90 binding site that is absent in ACT1-D10N. Therefore, whereas ACT1-D10N is a dead protein that is unable to transduce IL-17 signals for gene expression, ACT1-D19N is fully responsive to IL-17. Intriguingly, the two ACT1 isoforms are differentially expressed in ACT1(D10N/D10N) fibroblasts and T cells. Fibroblasts express both isoforms equally, enabling ACT1-D19N to compensate for the loss of ACT1-D10N function. ACT1(D10N/D10N) T cells, however, express predominantly ACT1-D10N. Lacking this compensatory mechanism, ACT1(D10N/D10N) T cells behave like ACT1-deficient T cells, exhibiting a dysregulated and hyperactive Th17 phenotype with overproduction of IL-22 and IL-17. The hyperactive Th17 response combined with fully responsive fibroblasts likely synergized to contribute to psoriasis susceptibility in SNP-D10N patients. PMID:25024377

  17. Some impacts of the 1990 Clean Air Act and state clean-air regulations on the fertilizer industry

    SciTech Connect

    Breed, C.E.; Kerns, O.S.

    1992-12-01

    The Clean Air Act amendments of 1990 will intensify national efforts to reduce air pollution. They will have major impacts an governmental agencies and on industrial and commercial facilities throughout the country. As with other industries, it is essential for fertilizer dealers and producers to understand how these changes to the Clean Air Act can significantly change the way they do business. This paper is proffered as an overview of ways in which the 1990 amendments to the Clean Air Act may impact the fertilizer industry. The nonattainment, toxics, and permit provisions of the amended act will be three areas of particular concern to the fertilizer industry. Implementation of the new regulatory requirements of this legislation promises to be a long and onerous process for all concerned. However, it appears that state and local regulations may have a much more profound impact on the fertilizer industry than the new Clean Air Act.

  18. Some impacts of the 1990 Clean Air Act and state clean-air regulations on the fertilizer industry

    SciTech Connect

    Breed, C.E.; Kerns, O.S.

    1992-12-31

    The Clean Air Act amendments of 1990 will intensify national efforts to reduce air pollution. They will have major impacts on governmental agencies and on industrial and commercial facilities throughout the country. As with other industries, it is essential for fertilizer dealers and producers to understand how these changes to the Clean Air Act can significantly change the way they do business. This paper is proffered as an overview of ways in which the 1990 amendments to the Clean Air Act may impact the fertilizer industry. The nonattainment, toxics, and permit provisions of the amended act will be three areas of particular concern to the fertilizer industry. Implementation of the new regulatory requirements of this legislation promises to be a long and onerous process for all concerned. However, it appears that state and local regulations may have a much more profound impact on the fertilizer industry than the new Clean Air Act.

  19. 13 CFR 120.150 - What are SBA's lending criteria?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... reasonably assure repayment. SBA will consider: (a) Character, reputation, and credit history of the... operate on a sound financial basis; (g) Potential for long-term success; (h) Nature and value...

  20. 13 CFR 120.150 - What are SBA's lending criteria?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... reasonably assure repayment. SBA will consider: (a) Character, reputation, and credit history of the... operate on a sound financial basis; (g) Potential for long-term success; (h) Nature and value...

  1. 13 CFR 120.150 - What are SBA's lending criteria?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... reasonably assure repayment. SBA will consider: (a) Character, reputation, and credit history of the... operate on a sound financial basis; (g) Potential for long-term success; (h) Nature and value...

  2. 13 CFR 120.150 - What are SBA's lending criteria?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... reasonably assure repayment. SBA will consider: (a) Character, reputation, and credit history of the... operate on a sound financial basis; (g) Potential for long-term success; (h) Nature and value...

  3. Cis-acting elements in its 3′ UTR mediate post-transcriptional regulation of KRAS

    PubMed Central

    Kim, Minlee; Kogan, Nicole; Slack, Frank J.

    2016-01-01

    Multiple RNA-binding proteins and non-coding RNAs, such as microRNAs (miRNAs), are involved in post-transcriptional gene regulation through recognition motifs in the 3′ untranslated region (UTR) of their target genes. The KRAS gene encodes a key signaling protein, and its messenger RNA (mRNA) contains an exceptionally long 3′ UTR; this suggests that it may be subject to a highly complex set of regulatory processes. However, 3′ UTR-dependent regulation of KRAS expression has not been explored in detail. Using extensive deletion and mutational analyses combined with luciferase reporter assays, we have identified inhibitory and stabilizing cis-acting regions within the KRAS 3′ UTR that may interact with miRNAs and RNA-binding proteins, such as HuR. Particularly, we have identified an AU-rich 49-nt fragment in the KRAS 3′ UTR that is required for KRAS 3′ UTR reporter repression. This element contains a miR-185 complementary element, and we show that overexpression of miR-185 represses endogenous KRAS mRNA and protein in vitro. In addition, we have identified another 49-nt fragment that is required to promote KRAS 3′ UTR reporter expression. These findings indicate that multiple cis-regulatory motifs in the 3′ UTR of KRAS finely modulate its expression, and sequence alterations within a binding motif may disrupt the precise functions of trans-regulatory factors, potentially leading to aberrant KRAS expression. PMID:26930719

  4. Alternative 3'UTRs act as scaffolds to regulate membrane protein localization

    PubMed Central

    Berkovits, Binyamin D.; Mayr, Christine

    2015-01-01

    About half of human genes use alternative cleavage and polyadenylation (ApA) to generate mRNA transcripts that differ in the length of their 3' untranslated regions (3'UTRs) while producing the same protein 1–3. Here we show in human cell lines that alternative 3' UTRs differentially regulate the localization of membrane proteins. The long 3'UTR of CD47 enables efficient cell surface expression of CD47 protein, whereas the short 3'UTR primarily localizes CD47 protein to the endoplasmic reticulum. CD47 protein localization occurs post-translationally and independently of RNA localization. In our model of 3' UTR-dependent protein localization, the long 3' UTR of CD47 acts as a scaffold to recruit a protein complex containing the RNA-binding protein HuR (also known as ELAVL1) and SET4 to the site of translation. This facilitates interaction of SET with the newly translated cytoplasmic domains of CD47 and results in subsequent translocation of CD47 to the plasma membrane via activated RAC1 5. We also show that CD47 protein has different functions depending on whether it was generated by the short or long 3'UTR isoforms. Thus, ApA contributes to the functional diversity of the proteome without changing the amino acid sequence. 3' UTR-dependent protein localization has the potential to be a widespread trafficking mechanism for membrane proteins because HuR binds to thousands of mRNAs6–9, and we show that the long 3' UTRs of CD44, ITGA1 and TNFRSF13C, which are bound by HuR, increase surface protein expression compared to their corresponding short 3' UTRs. We propose that during translation the scaffold function of 3' UTRs facilitates binding of proteins to nascent proteins to direct their transport or function—and that this role of 3' UTRs can be regulated by ApA. PMID:25896326

  5. Impact of Clean Air Act Regulations on Nitrogen Fate and Transport in the Neuse River Basin

    NASA Astrophysics Data System (ADS)

    Gabriel, M. C.; Knightes, C. D.; Dennis, R. L.; Cooter, E. J.

    2012-12-01

    This study investigated impacts of Clean Air Act Amendment (CAAA) NOx emissions regulations on the fate and transport of nitrogen for two watersheds in the Neuse River Basin, North Carolina, USA from 1990 to 2020. The Soil and Water Assessment Tool (SWAT) and the Community Multi-scale Air Quality (CMAQ) modeling system were used. CMAQ simulated atmospheric chemical transport and nitrogen deposition. This data was entered into SWAT which simulated watershed hydrology and water quality. Two cases were investigated: one that incorporates CAAA regulatory emissions controls in CMAQ simulation (with) and a second case that does not (without). SWAT model results forecasted a 70% decrease in inorganic nitrogen discharge from the Little River watershed and a 50% decrease for the Nahunta watershed by 2020 under the emission control (with) scenario. Denitrification and plant nitrogen uptake played important roles in nitrogen discharge from each watershed. The nitrogen discharge response time following a change in atmospheric nitrogen deposition was 4 years for the Nahunta watershed and 2 years for the Little River watershed. The longer response time for Nahunta is primarily due to a higher percentage of soybean land cover (22.5% [Nahunta]; 1.6% [Little River]). Agricultural land covers had varied nitrogen response times to changes in atmospheric deposition, particularly for soybean, hay and corn. The studied watersheds retained >80% of all nitrogen delivered by agriculture fertilization, biological fixation and atmospheric deposition.

  6. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES... objective intent; (3) The manufacture of the HCT/P does not involve the combination of the cells or tissues... not have a systemic effect and is not dependent upon the metabolic activity of living cells for...

  7. 21 CFR 1271.10 - Are my HCT/P's regulated solely under section 361 of the PHS Act and the regulations in this part...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES... objective intent; (3) The manufacture of the HCT/P does not involve the combination of the cells or tissues... not have a systemic effect and is not dependent upon the metabolic activity of living cells for...

  8. 29 CFR 102.117 - Freedom of Information Act Regulations: Board materials and formal documents available for public...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 2 2011-07-01 2011-07-01 false Freedom of Information Act Regulations: Board materials and formal documents available for public inspection and copying; requests for described records; time limit for response; appeal from denial of request; fees for document search and duplication; files and records not subject to inspection....

  9. 75 FR 8844 - Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations-Reports of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY 31 CFR Part 103 RIN 1506-AB08 Financial Crimes Enforcement Network; Amendment to the Bank Secrecy Act Regulations--Reports of Foreign Financial Accounts AGENCY: Financial Crimes Enforcement Network...

  10. 78 FR 6407 - Ability-to-Repay and Qualified Mortgage Standards Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... underwriting standards. See 73 FR 44524 (July 30, 2008). The following discussion provides background... rulemaking authority by section 1029 of the Dodd- Frank Act). 76 FR 79768 (Dec. 22, 2011). This rule did not... Regulation Z. See 60 FR 15463 (Mar. 24, 1995). In particular, Sec. 226.32(e)(1) \\42\\ implemented TILA...

  11. 78 FR 23171 - Amendments to the 2013 Escrows Final Rule Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-18

    ... loans made in ``rural'' or ``underserved'' areas. \\1\\ 78 FR 4726 (Jan. 22, 2013). \\2\\ The other rules...) (2013 ATR Final Rule), 78 FR 6407; High-Cost Mortgages and Homeownership Counseling Amendments to the... Settlement Procedures Act (Regulation X) (2013 HOEPA Final Rule), 78 FR 6855; Disclosure and...

  12. 75 FR 29526 - Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission 61,161; Docket No. RM93-11-000] Revisions to Oil Pipeline Regulations Pursuant to the Energy Policy Act of 1992; Notice of Annual Change in the Producer Price Index for Finished Goods May 19, 2010. The...

  13. 78 FR 60381 - Amendments to the 2013 Mortgage Rules Under the Equal Credit Opportunity Act (Regulation B), Real...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-01

    ... February 15, 2013, at 78 FR 11280. FOR FURTHER INFORMATION CONTACT: Whitney Patross, Attorney; Richard... Under the Truth in Lending Act (Regulation Z), 78 FR 4726 (Jan. 22, 2013) (2013 Escrows Final Rule...), 78 FR 6856 (Jan. 31, 2013) (2013 HOEPA Final Rule), and Ability-to-Repay and Qualified...

  14. 9 CFR 331.3 - States designated under paragraph 301(c) of the Act; application of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false States designated under paragraph 301(c) of the Act; application of regulations. 331.3 Section 331.3 Animals and Animal Products FOOD... DESIGNATED STATES AND TERRITORIES; AND FOR DESIGNATION OF ESTABLISHMENTS WHICH ENDANGER PUBLIC HEALTH AND...

  15. 9 CFR 381.222 - States designated under paragraph 5(c) of the Act; application of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false States designated under paragraph 5(c) of the Act; application of regulations. 381.222 Section 381.222 Animals and Animal Products FOOD... Designating Establishments With Operations Which Would Clearly Endanger the Public Health; Disposition...

  16. 78 FR 63408 - Petition To Amend Animal Welfare Act Regulations To Prohibit Public Contact With Big Cats, Bears...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-24

    ... 5, 2013 (78 FR 47215) is reopened. We will consider all comments that we receive on or before.... SUPPLEMENTARY INFORMATION: On August 5, 2013, we published in the Federal Register (78 FR 47215-47217, Docket No... Animal Welfare Act Regulations To Prohibit Public Contact With Big Cats, Bears, and Nonhuman...

  17. 78 FR 30739 - Amendments to the 2013 Escrows Final Rule under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    .... \\1\\ 78 FR 4726 (Jan. 22, 2013). \\2\\ The other rules include: Ability-to-Repay and Qualified Mortgage Standards under the Truth in Lending Act (Regulation Z) (2013 ATR Final Rule), 78 FR 6407 (Jan. 30, 2013...) (2013 HOEPA Final Rule), 78 FR 6855 (Jan. 31, 2013); Disclosure and Delivery Requirements for Copies...

  18. 75 FR 34271 - Federal Acquisition Regulation; FAR Case 2009-010, American Recovery and Reinvestment Act of 2009...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... Federal Register at 74 FR 14636 on March 31, 2009, with a request for comments by June 1, 2009. The... the Federal Register at 74 FR 14636 on March 31, 2009, is adopted as a final rule with the following... Acquisition Regulation; FAR Case 2009-010, American Recovery and Reinvestment Act of 2009 (the Recovery...

  19. Degradation of trinitroglycerin (TNG) using zero-valent iron nanoparticles/nanosilica SBA-15 composite (ZVINs/SBA-15).

    PubMed

    Saad, Rabih; Thiboutot, Sonia; Ampleman, Guy; Dashan, Wang; Hawari, Jalal

    2010-11-01

    Trinitroglycerin (TNG) is an industrial chemical mostly known for its clinical use in treating angina and manufacturing dynamite. The wide manufacture and application of TNG has led to contamination of vast areas of soil and water. The present study describes degradation of TNG with zero-valent iron nanoparticles (ZVINs) in water either present alone or stabilized on nanostructured silica SBA-15 (Santa Barbara Amorphous No. 15). The BET surface areas of ZVINs/SBA-15 (275.1 m2 g(-1)), as determined by nitrogen adsorption-desorption isotherms, was much larger than the non-stabilized ZVINs (82.0 m2 g(-1)). X-ray diffraction (XRD) showed that iron in both ZVINs and ZVINs/SBA-15 was present mostly in the α-Fe0 crystalline form considered responsible for TNG degradation. Transmission Electron Microscopy (TEM) showed that iron nanoparticles were well dispersed on the surface of the nanosilica support. Both ZVINs and ZVINs/SBA-15 degraded TNG (100%) in water to eventually produce glycerol and ammonium. The reaction followed pseudo-first-order kinetics and was faster with ZVINs/SBA-15 (k1 0.83 min(-1)) than with ZVINs (k1 0.228 min(-1)). The corresponding surface-area normalized rate constants, knorm, were 0.36 and 0.33 L h(-1) m(-2) for ZVINs/SBA-15 and ZVINs, respectively. The ZVINs/SBA-15 retained its original degradation efficiency of TNG after repeatedly reacting with fresh nitrate ester for five successive cycles. The rapid and efficient transformation of TNG with ZVINs/SBA-15, combined with excellent sustained reactivity, makes the nanometal an ideal choice for the clean up of water contaminated with TNG. PMID:20801482

  20. Transient colloidal stability controls the particle formation of SBA-15.

    PubMed

    Ruan, Juanfang; Kjellman, Tomas; Sakamoto, Yasuhiro; Alfredsson, Viveka

    2012-08-01

    A hypothesis about (transient) colloidal stability as a controlling mechanism for particle formation in SBA-15 is presented. The hypothesis is based on results from both in situ and ex situ investigations, including cryogenic transmission electron microscopy (cryo-TEM), UV-vis spectroscopy, and dynamic light scattering (DLS). Cryo-TEM images show that particles grow via the formation of silica-Pluronic-water "flocs", which coalesce in a seemingly arbitrary manner. Despite this, the final material consists of well-defined particles with a small size distribution. We argue that the interface between the flocs and surrounding media is covered by Pluronic molecules, which provide steric stabilization. As the flocs grow, the coverage of polymers at the interface is increased until a stable size is reached, and that regulates the particle size. By targeting the characteristics of the Pluronic molecules, during the on-going synthesis, the hypothesis is tested. The results are consistent with the concept of (transient) colloidal stability. PMID:22758927

  1. Transient Colloidal Stability Controls the Particle Formation of SBA-15

    PubMed Central

    2012-01-01

    A hypothesis about (transient) colloidal stability as a controlling mechanism for particle formation in SBA-15 is presented. The hypothesis is based on results from both in situ and ex situ investigations, including cryogenic transmission electron microscopy (cryo-TEM), UV–vis spectroscopy, and dynamic light scattering (DLS). Cryo-TEM images show that particles grow via the formation of silica–Pluronic–water “flocs”, which coalesce in a seemingly arbitrary manner. Despite this, the final material consists of well-defined particles with a small size distribution. We argue that the interface between the flocs and surrounding media is covered by Pluronic molecules, which provide steric stabilization. As the flocs grow, the coverage of polymers at the interface is increased until a stable size is reached, and that regulates the particle size. By targeting the characteristics of the Pluronic molecules, during the on-going synthesis, the hypothesis is tested. The results are consistent with the concept of (transient) colloidal stability. PMID:22758927

  2. 76 FR 10527 - Regulatory Flexibility Act: Section 610 Review of National Organic Program Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-25

    ... regulations implementing the National Organic Program (NOP) were published December 21, 2000 (65 FR 80548... FR 14827), its schedule to review certain regulations, including the NOP regulations, under criteria... Organic Program Regulations AGENCY: Agricultural Marketing Service, USDA. ACTION: Review and request...

  3. 13 CFR 107.440 - Standards governing prior SBA approval for a proposed transfer of Control.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... prior SBA approval for a proposed transfer of Control. SBA approval is contingent upon full disclosure... assume, in writing, personal liability for your Leverage, effective only in the event of their direct...

  4. 76 FR 78130 - Mortgage Acts and Practices-Advertising (Regulation N); Mortgage Assistance Relief Services...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-16

    ... Advance Notice of Proposed Rulemaking: Mortgage Acts and Practices, 74 FR 26118 (June 1, 2009), that.... See 76 FR 43569 (July 21, 2011). The Omnibus Appropriations Act, as amended, authorizes the Bureau to... response to a notice published at 76 FR 75825 (Dec. 5, 2011) concerning its efforts to identify...

  5. 75 FR 12377 - Native American Graves Protection and Repatriation Act Regulations-Disposition of Culturally...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... first rules to implement the Act on December 4, 1995 (43 CFR part 10, 60 FR 62158). --We published rules for assessing civil penalties under the Act on April 3, 2003 (43 CFR 10.12, 68 FR 16354). --We....13, 72 FR 13189). --We are publishing this rule today. --We are developing additional rules to...

  6. 78 FR 41835 - Inflation Adjustments to the Price-Anderson Act Financial Protection Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-12

    ... consistent with the Plain Writing Act as well as the Presidential Memorandum, ``Plain Language in Government... made the initial changes to the Price-Anderson Act amounts on October 27, 2005 (70 FR 61885), and the first periodic inflation adjustments on September 29, 2008 (73 FR 56451). This final rule makes...

  7. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2013-04-01 2013-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  8. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2010-04-01 2010-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  9. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2014-04-01 2014-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  10. 25 CFR 101.10 - Federal Reserve Regulation Z and Fair Credit Reporting Act.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... subject to the provisions of Federal Reserve Regulation Z (Truth In Lending, 12 CFR part 226; Pub. L. 91... 25 Indians 1 2011-04-01 2011-04-01 false Federal Reserve Regulation Z and Fair Credit Reporting... ACTIVITIES LOANS TO INDIANS FROM THE REVOLVING LOAN FUND § 101.10 Federal Reserve Regulation Z and...

  11. GP Supervisors' Experience in Supporting Self-Regulated Learning: A Balancing Act

    ERIC Educational Resources Information Center

    Sagasser, Margaretha H.; Kramer, Anneke W. M.; van Weel, Chris; van der Vleuten, Cees P. M.

    2015-01-01

    Self-regulated learning is essential for professional development and lifelong learning. As self-regulated learning has many inaccuracies, the need to support self-regulated learning has been recommended. Supervisors can provide such support. In a prior study trainees reported on the variation in received supervisor support. This study aims at…

  12. 13 CFR 127.403 - What happens if SBA verifies the concern's eligibility?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What happens if SBA verifies the concern's eligibility? 127.403 Section 127.403 Business Credit and Assistance SMALL BUSINESS....403 What happens if SBA verifies the concern's eligibility? If SBA verifies that the concern...

  13. 13 CFR 101.200 - When does SBA hire private counsel?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false When does SBA hire private counsel? 101.200 Section 101.200 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Employment of Private Counsel § 101.200 When does SBA hire private counsel? (a) Business loans. SBA may...

  14. 13 CFR 101.100 - What is the purpose of SBA?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What is the purpose of SBA? 101.100 Section 101.100 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.100 What is the purpose of SBA? The U.S. Small Business Administration (SBA) aids,...

  15. 13 CFR 120.1718 - SBA's right to assume Seller's responsibilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA's right to assume Seller's responsibilities. 120.1718 Section 120.1718 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools § 120.1718 SBA's right to...

  16. 7 CFR 4290.509 - Employment of USDA or SBA officials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Employment of USDA or SBA officials. 4290.509 Section... or SBA officials. (a) Without the Secretary's prior written approval, for a period of two years after... professional services, any person who: (1) Served as an officer, attorney, agent, or employee of SBA or...

  17. 13 CFR 124.204 - How does SBA process applications for 8(a) BD program admission?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false How does SBA process applications... Development Applying to the 8(a) Bd Program § 124.204 How does SBA process applications for 8(a) BD program... except those from ANC-owned applicants. SBA's Anchorage District Office will receive all...

  18. 13 CFR 125.27 - How will SBA process an SDVO protest?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false How will SBA process an SDVO... CONTRACTING PROGRAMS Protests Concerning SDVO SBCs § 125.27 How will SBA process an SDVO protest? (a) Notice of receipt of protest. Upon receipt of the protest, SBA will notify the contracting officer and...

  19. 7 CFR 4290.509 - Employment of USDA or SBA officials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Employment of USDA or SBA officials. 4290.509 Section... or SBA officials. (a) Without the Secretary's prior written approval, for a period of two years after... professional services, any person who: (1) Served as an officer, attorney, agent, or employee of SBA or...

  20. 13 CFR 127.404 - What happens if SBA is unable to verify a concern's eligibility?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... that it has 15 calendar days from the date it receives the notice to respond. (b) SBA determination... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What happens if SBA is unable to... Examinations § 127.404 What happens if SBA is unable to verify a concern's eligibility? (a) Notice of...

  1. 13 CFR 127.604 - How will SBA process an EDWOSB or WOSB status protest?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .... SBA will determine the EDWOSB or WOSB status of the protested concern within 15 business days after.... If SBA does not issue its determination within the 15-day period, the contracting officer may award... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false How will SBA process an EDWOSB...

  2. 13 CFR 120.1720 - SBA's right to review Pool Loan documents.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... underwriting documents, and any other documents SBA may require in writing within 15 calendar days of a written... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false SBA's right to review Pool Loan... LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan...

  3. 13 CFR 120.1718 - SBA's right to assume Seller's responsibilities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 calendar days of Seller's receipt of such request. SBA will notify the Obligor of the change in... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false SBA's right to assume Seller's... LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan...

  4. 13 CFR 126.803 - How will SBA process a HUBZone status protest?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How will SBA process a HUBZone status protest? 126.803 Section 126.803 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION HUBZONE PROGRAM Protests § 126.803 How will SBA process a HUBZone status protest? (a) Notice of receipt of protest. (1) SBA immediately will notify...

  5. 13 CFR 120.470 - What are SBA's additional requirements for SBLCs?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Form 14, or Finance Companies Blanket Bond, Standard Form 15, or such other form of coverage as SBA may... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What are SBA's additional... BUSINESS LOANS Lenders Small Business Lending Companies (sblc) § 120.470 What are SBA's...

  6. 13 CFR 120.223 - Subsidy recoupment fee payable to SBA by Borrower.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SBA by Borrower. 120.223 Section 120.223 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION... payable to SBA by Borrower. (a) The subsidy recoupment fee is payable to SBA when: (1) Loan has a maturity of 15 years or more. (2) Borrower makes a voluntary prepayment (or several prepayments in...

  7. 13 CFR 126.803 - How will SBA process a HUBZone status protest?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .... (2) If SBA does not contact the contracting officer within 15 business days, the contracting officer... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false How will SBA process a HUBZone... HUBZONE PROGRAM Protests § 126.803 How will SBA process a HUBZone status protest? (a) Notice of receipt...

  8. 13 CFR 120.1720 - SBA's right to review Pool Loan documents.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA's right to review Pool Loan documents. 120.1720 Section 120.1720 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Establishment of SBA Secondary Market Guarantee Program for First Lien Position 504 Loan Pools § 120.1720 SBA's right to review Pool...

  9. 13 CFR 120.434 - What are SBA's requirements for loan pledges?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... satisfactory SBA performance, as determined by SBA in its discretion. The Lender's Risk Rating, among other... must retain an economic interest in and the ultimate risk of loss on the unguaranteed portion of the... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are SBA's requirements...

  10. 13 CFR 101.103 - Where are SBA's field offices located?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Where are SBA's field offices located? 101.103 Section 101.103 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.103 Where are SBA's field offices located? A list of SBA's field offices...

  11. 13 CFR 108.1230 - Draw-downs by NMVC Company under SBA's Leverage commitment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Draw-downs by NMVC Company under...) Conditional Commitments by Sba to Reserve Leverage for A Nmvc Company § 108.1230 Draw-downs by NMVC Company... submitting a request for a draw against SBA's Leverage commitment, you authorize SBA, or any agent or...

  12. 13 CFR 108.1230 - Draw-downs by NMVC Company under SBA's Leverage commitment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Draw-downs by NMVC Company under...) Conditional Commitments by Sba to Reserve Leverage for A Nmvc Company § 108.1230 Draw-downs by NMVC Company... submitting a request for a draw against SBA's Leverage commitment, you authorize SBA, or any agent or...

  13. 13 CFR 108.1230 - Draw-downs by NMVC Company under SBA's Leverage commitment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Draw-downs by NMVC Company under...) Conditional Commitments by Sba to Reserve Leverage for A Nmvc Company § 108.1230 Draw-downs by NMVC Company... submitting a request for a draw against SBA's Leverage commitment, you authorize SBA, or any agent or...

  14. 13 CFR 101.105 - Who may use SBA's official seal and for what purpose?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Who may use SBA's official seal... ADMINISTRATION ADMINISTRATION Overview § 101.105 Who may use SBA's official seal and for what purpose? (a) General. This section describes the official seal of the SBA and prescribes rules for its use....

  15. 13 CFR 108.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false SBA authority to collect or compromise its claims. 108.1710 Section 108.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION...) Miscellaneous § 108.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions...

  16. 13 CFR 107.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA authority to collect or compromise its claims. 107.1710 Section 107.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION... § 107.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions and for...

  17. 13 CFR 120.524 - When is SBA released from liability on its guarantee?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... liability on its guarantee? 120.524 Section 120.524 Business Credit and Assistance SMALL BUSINESS... Purchase of A Guaranteed Portion § 120.524 When is SBA released from liability on its guarantee? (a) SBA is... from its guarantee only if the Lender fails to request that SBA purchase the guarantee within 180...

  18. 13 CFR 107.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false SBA authority to collect or compromise its claims. 107.1710 Section 107.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION... § 107.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions and for...

  19. 13 CFR 108.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA authority to collect or compromise its claims. 108.1710 Section 108.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION...) Miscellaneous § 108.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions...

  20. 13 CFR 107.650 - Requirement to report portfolio valuations to SBA.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... valuations to SBA. 107.650 Section 107.650 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL... Reporting Requirements for Licensees § 107.650 Requirement to report portfolio valuations to SBA. You must... valuations to SBA within 90 days of the end of the fiscal year in the case of annual valuations, and...

  1. 13 CFR 101.105 - Who may use SBA's official seal and for what purpose?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Who may use SBA's official seal... ADMINISTRATION ADMINISTRATION Overview § 101.105 Who may use SBA's official seal and for what purpose? (a) General. This section describes the official seal of the SBA and prescribes rules for its use....

  2. 13 CFR 101.105 - Who may use SBA's official seal and for what purpose?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Who may use SBA's official seal... ADMINISTRATION ADMINISTRATION Overview § 101.105 Who may use SBA's official seal and for what purpose? (a) General. This section describes the official seal of the SBA and prescribes rules for its use....

  3. 13 CFR 101.105 - Who may use SBA's official seal and for what purpose?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Who may use SBA's official seal... ADMINISTRATION ADMINISTRATION Overview § 101.105 Who may use SBA's official seal and for what purpose? (a) General. This section describes the official seal of the SBA and prescribes rules for its use....

  4. 13 CFR 101.105 - Who may use SBA's official seal and for what purpose?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Who may use SBA's official seal... ADMINISTRATION ADMINISTRATION Overview § 101.105 Who may use SBA's official seal and for what purpose? (a) General. This section describes the official seal of the SBA and prescribes rules for its use....

  5. 13 CFR 107.1570 - Distributions by Licensee-optional Distribution to private investors and SBA.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Distribution to private investors and SBA. 107.1570 Section 107.1570 Business Credit and Assistance SMALL... private investors and SBA. If you have outstanding Participating Securities or Earmarked Assets, you may... and your private investors in proportion to the capital contributions of each. You must notify SBA...

  6. 13 CFR 107.1570 - Distributions by Licensee-optional Distribution to private investors and SBA.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Distribution to private investors and SBA. 107.1570 Section 107.1570 Business Credit and Assistance SMALL... private investors and SBA. If you have outstanding Participating Securities or Earmarked Assets, you may... and your private investors in proportion to the capital contributions of each. You must notify SBA...

  7. 13 CFR 107.1570 - Distributions by Licensee-optional Distribution to private investors and SBA.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Distribution to private investors and SBA. 107.1570 Section 107.1570 Business Credit and Assistance SMALL... private investors and SBA. If you have outstanding Participating Securities or Earmarked Assets, you may... and your private investors in proportion to the capital contributions of each. You must notify SBA...

  8. 13 CFR 107.1570 - Distributions by Licensee-optional Distribution to private investors and SBA.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Distribution to private investors and SBA. 107.1570 Section 107.1570 Business Credit and Assistance SMALL... private investors and SBA. If you have outstanding Participating Securities or Earmarked Assets, you may... and your private investors in proportion to the capital contributions of each. You must notify SBA...

  9. 13 CFR 107.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false SBA authority to collect or compromise its claims. 107.1710 Section 107.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION... § 107.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions and for...

  10. 13 CFR 108.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false SBA authority to collect or compromise its claims. 108.1710 Section 108.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION...) Miscellaneous § 108.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions...

  11. 13 CFR 107.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false SBA authority to collect or compromise its claims. 107.1710 Section 107.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION... § 107.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions and for...

  12. 13 CFR 120.524 - When is SBA released from liability on its guarantee?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... liability on its guarantee? 120.524 Section 120.524 Business Credit and Assistance SMALL BUSINESS... Purchase of A Guaranteed Portion § 120.524 When is SBA released from liability on its guarantee? (a) SBA is... from its guarantee only if the Lender fails to request that SBA purchase the guarantee within 180...

  13. 13 CFR 120.524 - When is SBA released from liability on its guarantee?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... liability on its guarantee? 120.524 Section 120.524 Business Credit and Assistance SMALL BUSINESS... Purchase of A Guaranteed Portion § 120.524 When is SBA released from liability on its guarantee? (a) SBA is... from its guarantee only if the Lender fails to request that SBA purchase the guarantee within 180...

  14. 13 CFR 108.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false SBA authority to collect or compromise its claims. 108.1710 Section 108.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION...) Miscellaneous § 108.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions...

  15. 13 CFR 107.1710 - SBA authority to collect or compromise its claims.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false SBA authority to collect or compromise its claims. 107.1710 Section 107.1710 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION... § 107.1710 SBA authority to collect or compromise its claims. SBA may, upon such conditions and for...

  16. 13 CFR 108.1140 - NMVC Company's acceptance of SBA remedies under § 108.1810.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false NMVC Company's acceptance of SBA... ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM SBA Financial Assistance for NMVC Companies (Leverage) General Information About Obtaining Leverage § 108.1140 NMVC Company's acceptance of SBA remedies...

  17. 13 CFR 120.1400 - Grounds for enforcement actions-SBA Lenders.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Grounds for enforcement actions-SBA Lenders. 120.1400 Section 120.1400 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Risk-Based Lender Oversight Enforcement Actions § 120.1400 Grounds for enforcement actions—SBA Lenders. (a) Agreement. By making SBA...

  18. 13 CFR 120.542 - Payment by SBA of legal fees and other expenses.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Liquidator that disagrees with a decision by SBA to decline to reimburse all, or a portion, of the legal fees... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Payment by SBA of legal fees and... of A Guaranteed Portion § 120.542 Payment by SBA of legal fees and other expenses. (a) Legal fees...

  19. 13 CFR 101.100 - What is the purpose of SBA?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... business development assistance. For a more detailed description of the functions of SBA see The United....100 Section 101.100 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.100 What is the purpose of SBA? The U.S. Small Business Administration (SBA) aids,...

  20. 13 CFR 101.100 - What is the purpose of SBA?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... business development assistance. For a more detailed description of the functions of SBA see The United....100 Section 101.100 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.100 What is the purpose of SBA? The U.S. Small Business Administration (SBA) aids,...

  1. The Regulation of Medical Computer Software as a “Device” under the Food, Drug, and Cosmetic Act

    PubMed Central

    Brannigan, Vincent

    1986-01-01

    Recent developments in computer software have raised the possibility that federal regulators may claim to control medical computer software as a “device” under the Food, Drug and Cosmetic Act. The purpose of this paper is to analyze the FDCA to determine whether computer software is included in the statutory scheme, examine constitutional arguments relating to computer software, and discuss regulatory principles that should be taken into account when deciding appropriate regulation. This paper is limited to computer program output used by humans in deciding appropriate medical therapy for a patient.

  2. 78 FR 20291 - Fisheries of the United States; Billfish Conservation Act of 2012 Implementing Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-04

    ...NMFS issues this advance notice of proposed rulemaking (ANPR) to provide background information and request public comment on potential issues related to the implementation of the Billfish Conservation Act of...

  3. Direct synthesis and characterization of phenyl-functionalized SBA-15

    NASA Astrophysics Data System (ADS)

    Wang, Xue-mei; Du, Xin-zhen; Li, Chun-lan; Cao, Xu

    2008-04-01

    Phenyl-functionalized SBA-15 materials (Ph-SBA-15) were directly synthesized by using tri-block copolymer Pluronic P123 as templating agent under acidic conditions. The samples were characterized by Fourier transform infrared (FT-IR) spectra, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermogravimetry analysis (TGA) and N 2 adsorption-desorption. The results show that the phenyl groups are covalently attached to the pore wall of SBA-15 after modification. The functionalized materials still preserve a desirable two-dimensional P6 mm hexagonal structure and have large specific surface area and pore volume although the molar ratio of phenyltrimethoxysilane in total silica precursors is as high as 23.0%.

  4. Grafting of alumina on SBA-15: effect of surface roughness.

    PubMed

    Zukal, A; Siklová, H; Cejka, J

    2008-09-01

    Alumina-grafted materials were prepared by postsynthesis alumination of mesoporous SBA-15 silica in an aqueous solution of aluminum chlorhydrol. Prepared samples were characterized by nitrogen adsorption, scanning electron microscopy, X-ray powder diffraction, and (27)Al magic-angle-spinning NMR. The successive grafting of alumina on SBA-15 leads to a gradual filling of the corona surrounding the mesopores. As a consequence smoothing of the mesopore surface takes place. The in-depth analysis of nitrogen adsorption data proves that the alpha s method affords real values of the structure parameters, while the Kruk, Jaroniec, and Sayari (KJS) procedure based on the BJH algorithm provides only effective data corresponding to cylindrical mesopores of smooth geometrical surface. The quantification of the roughness of the SBA-15 mesopore surface based on the comparison of data obtained from the alpha s plot and KJS method was carried out. PMID:18683961

  5. 13 CFR 124.1013 - How does SBA make disadvantaged status determinations in considering an SDB protest?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SBA determination within 15 working days after the SBA's receipt of the protest, the contracting... the contract until: (i) The SBA has made an SDB determination, or (ii) 15 working days have expired since SBA's receipt of a protest and the contracting officer has not agreed to an extension of the...

  6. Regulation of caulimovirus gene expression and the involvement of cis-acting elements on both viral transcripts.

    PubMed

    Scholthof, H B; Wu, F C; Gowda, S; Shepherd, R J

    1992-09-01

    In a further analysis of gene regulation of figwort mosaic virus (FMV), a caulimovirus, we studied transient gene expression with modified viral genomes in Nicotiana edwardsonii cell suspension protoplasts. The results demonstrated that the presence of the promoter for the full-length RNA interferes with expression from the separate downstream promoter for gene VI. In addition, expression of gene VI was inhibited by cis-acting sequences within gene VI itself. Both inhibitory effects could be partially relieved by coelectroporation with a plasmid that produces gene VI protein, demonstrating that expression of gene VI is transactivated by its own product. Subsequent expression studies with partially redundant FMV plasmids containing a reporter gene in frame with gene IV showed that efficient transactivation of CAT expression relies on a cis-acting element inside the downstream gene VI. Insertions of a transcriptional terminator upstream of the cis-acting element for premature termination of transcription showed that the cis-acting region is not a DNA element but is active only as a feature of the RNA transcript. We conclude that the cis-acting element, together with the transacting gene VI product, enhances expression of all major genes, including gene VI, from the polycistronic mRNA and the separate mRNA for gene VI. PMID:1529539

  7. Sol Gel-Derived SBA-16 Mesoporous Material

    PubMed Central

    Rivera-Muñoz, Eric M.; Huirache-Acuña, Rafael

    2010-01-01

    The aim of this article is to review current knowledge related to the synthesis and characterization of sol gel-derived SBA-16 mesoporous silicas, as well as a review of the state of the art in this issue, to take stock of knowledge about current and future applications. The ease of the method of preparation, the orderly structure, size and shape of their pores and control, all these achievable through simple changes in the method of synthesis, makes SBA-16 a very versatile material, potentially applicable in many areas of science and molecular engineering of materials. PMID:20957080

  8. 76 FR 10234 - Amendment to the Bank Secrecy Act Regulations-Reports of Foreign Financial Accounts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... Regulations (CFR) for BSA regulations. (See 75 FR 65806 (October 26, 2010) (Transfer and Reorganization of... draft of which appeared in the Federal Register as an attachment to the NPRM. \\3\\ See 75 FR 8844... foreign financial institution.\\5\\ \\4\\ 75 FR 8851 (February 26, 2010) (Emphasis added). \\5\\ A revised...

  9. 75 FR 51420 - Removing Regulations Implementing the Fish and Wildlife Conservation Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-20

    ... implementing regulations (50 CFR 83) in the May 6, 2010, Federal Register (75 FR 24862) and invited public... with Native American Tribal Governments'' (59 FR 22951), E.O. 13175, and 512 DM 2. We have determined... Fish and Wildlife Service 50 CFR Part 83 RIN 1018-AX00 Removing Regulations Implementing the Fish...

  10. 76 FR 64049 - Bank Secrecy Act Regulations: Definition of “Monetary Instrument”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-17

    ... Code of Federal Regulations for the BSA regulations. See 75 FR 65806 (October 26, 2010) (Transfer and... rule could be proposed by creating a regulatory framework and definition of prepaid access. \\10\\ 75 FR... regarding reporting of the international transport of prepaid access, see 75 FR 36593. \\11\\ 76 FR 45403....

  11. Twenty Years of the Dietary Supplement Health and Education Act--How Should Dietary Supplements Be Regulated?

    PubMed

    Wallace, Taylor C

    2015-08-01

    The Dietary Supplement Health and Education Act (DSHEA) of 1994 defines the FDA's statutory authority to regulate dietary supplement products in the United States. The dietary supplement industry has rapidly expanded since 1994, presenting an obvious need for "DSHEA 2.0." Current regulations surrounding dietary supplements have been increasingly and reasonably scrutinized, given their widespread use by over one-half of the US population as well as highly publicized safety concerns over the past 20 y. As the market continues to expand and evolve, so too must the laws that protect consumers from potential harm and misleading communication. This article is meant to begin a scientific dialogue on how regulations may be improved to provide both ease of access and safer products to the consumer by focusing on 4 topics: premarket approval, label claims, current Good Manufacturing Practices, and adverse event reporting. PMID:26063064

  12. Synthesis, characterization, and catalytic properties of Ti-containing SBA-15 mesoporous materials

    NASA Astrophysics Data System (ADS)

    Dang, Phuong T.; Le, Hy G.; Hoang, Duc V.; Hoang, Yen; Dinh, Thang C.; Bui, Linh H.; Tran, Hoa T. K.; Nguyen, Phu H.; Vu, Tuan A.

    2006-12-01

    Titanium-containing SBA-15 mesoporous materials with Si/Ti molar ratios of 25, 50 and 100 (Ti-SBA-15) were successfully prepared by direct synthesized method using P123 as surfactant. The samples were characterized by XRD, BET, TEM and UV-Vis. It revealed at low Ti-loading (Si/Ti of 50-100), titanium was completely incorporated into SBA-15 framework, whereas at high Ti-loading (Si/Ti of 25) titanium was partially incorporated into SBA-15 framework, one part of Ti existed as extra-framework Ti (anatase phase). For comparison, Ti impregnated on Si-SBA-15 (Ti/SBA-15) was also prepared by postsynthesis method. In (*)this case, titanium was well dispersed onto the surface of SBA-15. The catalytic activities of Ti-SBA-15 with different Ti-content and Ti/SBA-15 samples were tested in the photocatalytic oxidation of red-phenol and in the photocatalytic reduction of Cr(VI) to Cr(III). The catalytic results showed that both the Ti-SBA-15 and Ti/SBA-15 solids are also the good catalysts for total photooxidation of red phenol. Especially, the tetrahedral coordinated titanium can oxidize red phenol much deeper than well dispersed titanium particles does. For photocatalytic reduction, the activities mainly depend on the number of Ti, not the state of Ti.

  13. Fnr (EtrA) acts as a fine-tuning regulator of anaerobic metabolism in Shewanella oneidensis MR-1

    SciTech Connect

    Cruz-Garza, Claribel; Murray, Alison E.; Rodrigues, Jorge L.M.; Gralnick, Jeffrey A.; McCue, Lee Ann; Romine, Margaret F.; Loffler, F. E.; Tiedje, James M.

    2011-03-30

    EtrA in Shewanella oneidensis MR-1, a model organism for study of adaptation to varied redox niches, shares 73.6% and 50.8% amino acid sequence identity with the oxygen-sensing regulators Fnr in E. coli and Anr in Pseudomonas aeruginosa, respectively; however, its regulatory role of anaerobic metabolism in Shewanella spp. is not well understood. The expression of the nap genes, nrfA, cymA and hcp was significantly reduced in etrA deletion mutant EtrA7-1; however, limited anaerobic growth and nitrate reduction occurred, suggesting that multiple regulators control nitrate reduction in this strain. Dimethyl sulfoxide (DMSO) and fumarate reductase gene expression was down regulated at least 2-fold and the EtrA7-1 mutant grew poorly with fumarate and dimethyl sulfoxide (DMSO), suggesting both respiratory pathways are under EtrA control. Transcript analysis further suggested a role of EtrA in prophage activation and down regulation of genes implicated in aerobic metabolism. In contrast to previous studies that attributed a minor regulatory role to EtrA in Shewanella spp., this study demonstrates that EtrA acts as a global transcriptional regulator and confers physiological advantages to strain MR-1 under certain growth conditions. In conjunction with other regulators, EtrA fine-tunes the expression of genes involved in anaerobic metabolism in S. oneidensis strain MR-1.

  14. 75 FR 63425 - Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... the responsible employer, and that he or she is disabled as a result of the illness. (3) Hearing loss. If the individual claims compensation for hearing loss, the date of injury is the date the individual... and Harbor Workers' Compensation Act: Recreational Vessels, published on August 17, 2010 (75 FR...

  15. 75 FR 46859 - Lacey Act Implementation Plan; Definitions for Exempt and Regulated Articles

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-04

    ... is being phased in, as described in two notices we published in the Federal Register (74 FR 5911-5913 and 74 FR 45415- 45418, Docket No. APHIS-2008-0119). Under the Act, ``Plant'' means: ``Any wild member... an appendix to the Convention on International Trade in Endangered Species of Wild Fauna and...

  16. 76 FR 78483 - S.A.F.E. Mortgage Licensing Act (Regulations G & H)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    ... part of the public record and subject to public disclosure. Sensitive personal information, such as... for licensing loan originators that meets the requirements of the S.A.F.E. Act. \\1\\ 75 FR 44656 (July... this rule. See 76 FR 43569 (July 21, 2011). \\6\\ Public Law 111-203, section 1002(14)...

  17. Trans-acting factors regulate the expression of CD44 splice variants.

    PubMed Central

    Konig, H; Moll, J; Ponta, H; Herrlich, P

    1996-01-01

    Variant isoforms of the cell surface glycoprotein CD44 (CD44v) are expressed during development, in selected adult tissues and in certain metastatic tumor cells. CD44v differ from the standard isoform (CD44s) by up to ten additional exon sequences included by alternative splicing. By cell fusion experiments, we have obtained evidence for the existence of cell-type specific trans-acting factors recruiting CD44 variant exon sequences. Stable cell hybrids of CD44s and CD44v expressing cells indicated a dominant mechanism for variant-exon inclusion. In transient interspecies heterokaryons of human keratinocytes and rat fibroblasts, the ability of the keratinocytes to include all variant exon sequences in CD44 was conferred completely on the rat fibroblast nucleus. Fusions of cells with complex CD44 splice patterns do not permit interpretation of splice control by the relative abundance of a single trans-acting factor, but rather by (a) positively acting factor(s) recruiting variant exon sequences in the 3' to 5' direction and additional factors selecting individual exons. Since the pancreatic carcinoma cell line BSp73ASML (in contrast to the cervix carcinoma cell lines SiHa and ME180) could not transfer its specific splice pattern in cell fusions, we conclude that in some tumors, splicing is also controlled by mutation of cis-acting recognition sites. Images PMID:8670907

  18. 78 FR 36693 - Rules and Regulations Under the Fur Products Labeling Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-19

    ... guaranties annually. \\10\\ 78 FR 29263 (May 20, 2013). In light of the proposed amendments to the Textile... Act, 77 FR 57043 (Sept. 17, 2012). \\8\\ National Retail Federation Comment 00025 at 1-5, available at... Activities; Submission for OMB Review; Comment Request, 7 FR 10744 (Feb. 23, 2012). D. Projected...

  19. 76 FR 63817 - Disclosure of Information; Privacy Act Regulations; Notice and Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-14

    ... supervisory agency, for purposes of Sec. 309.6, to which exempt records could be disclosed.\\1\\ \\1\\ 76 FR 35963.... D. Plain Language Section 722 of the Gramm-Leach-Bliley Act (Pub. L. 106-102, 113 Stat. 1338, 1471), requires the Federal banking agencies to use plain language in all proposed and final rules published...

  20. 75 FR 58389 - Federal Acquisition Regulation; Information Collection; American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... Reporting Requirements for Prime Contractors AGENCY: Department of Defense (DOD), General Services... American Recovery and Reinvestment Act-Reporting Requirements--One Time Reporting Requirements for Prime... for which the burden is imposed on the prime contractor under the FAR requirements include...

  1. 75 FR 58388 - Federal Acquisition Regulation; Information Collection; American Recovery and Reinvestment Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... Reporting for Prime Contractors AGENCY: Department of Defense (DOD), General Services Administration (GSA... Recovery and Reinvestment Act--Quarterly Reporting for Prime Contractors. Public comments are particularly...- mail to ernest.woodson@gsa.gov . ] SUPPLEMENTARY INFORMATION: A. Purpose Quarterly Reporting for...

  2. 20 CFR 626.4 - Table of contents for the Job Training Partnership Act regulations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the Job Training Partnership Act, 20 CFR parts 626-638 and 1005, 1 is as follows: 1 Part 1005 was...—Center Operations 638.500Orientation program. 694.501Student handbook. 638.502Job Corps basic...

  3. Market effects of environmental regulation: coal, railroads, and the 1990 Clean Air Act

    SciTech Connect

    Busse, M.R.; Keohane, N.O.

    2007-01-01

    Many environmental regulations encourage the use of 'clean' inputs. When the suppliers of such an input have market power, environmental regulation will affect not only the quantity of the input used but also its price. We investigate the effect of the Title IV emissions trading program for sulfur dioxide on the market for low-sulfur coal. We find that the two railroads transporting coal were able to price discriminate on the basis of environmental regulation and geographic location. Delivered prices rose for plants in the trading program relative to other plants, and by more at plants near a low-sulfur coal source.

  4. Increasing the oral bioavailability of poorly water-soluble carbamazepine using immediate-release pellets supported on SBA-15 mesoporous silica

    PubMed Central

    Wang, Zhouhua; Chen, Bao; Quan, Guilan; Li, Feng; Wu, Qiaoli; Dian, Linghui; Dong, Yixuan; Li, Ge; Wu, Chuanbin

    2012-01-01

    Background and methods: The aim of this study was to develop an immediate-release pellet formulation with improved drug dissolution and adsorption. Carbamazepine, a poorly water-soluble drug, was adsorbed into mesoporous silica (SBA-15-CBZ) via a wetness impregnation method and then processed by extrusion/spheronization into pellets. Physicochemical characterization of the preparation was carried out by scanning electron microscopy, transmission electron microscopy, nitrogen adsorption, small-angle and wide-angle x-ray diffraction, and differential scanning calorimetry. Flowability and wettability of the drug-loaded silica powder were evaluated by bulk and tapped density and by the angle of repose and contact angle, respectively. The drug-loaded silica powder was formulated into pellets to improve flowability. Results: With maximum drug loading in SBA-15 matrices determined to be 20% wt, in vitro release studies demonstrated that the carbamazepine dissolution rate was notably improved from both the SBA-15 powder and the corresponding pellets as compared with the bulk drug. Correspondingly, the oral bioavailability of SBA-15-CBZ pellets was increased considerably by 1.57-fold in dogs (P < 0.05) compared with fast-release commercial carbamazepine tablets. Conclusion: Immediate-release carbamazepine pellets prepared from drug-loaded silica provide a feasible approach for development of a rapidly acting oral formulation for this poorly water-soluble drug and with better absorption. PMID:23209366

  5. Ethylene acts as a negative regulator of glucose induced lateral root emergence in Arabidopsis.

    PubMed

    Singh, Manjul; Gupta, Aditi; Laxmi, Ashverya

    2015-01-01

    Plants, being sessile organisms, are more exposed to the hazards of constantly changing environmental conditions globally. During the lifetime of a plant, the root system encounters various challenges such as obstacles, pathogens, high salinity, water logging, nutrient scarcity etc. The developmental plasticity of the root system provides brilliant adaptability to plants to counter the changes exerted by both external as well as internal cues and achieve an optimized growth status. Phytohormones are one of the major intrinsic factors regulating all aspects of plant growth and development both independently as well as through complex signal integrations at multiple levels. We have previously shown that glucose (Glc) and brassinosteroid (BR) signalings interact extensively to regulate lateral root (LR) development in Arabidopsis. (1) Auxin efflux as well as influx and downstream signaling components are also involved in Glc-BR regulation of LR emergence. Here, we provide evidence for involvement of ethylene signaling machinery downstream to Glc and BR in regulation of LR emergence. PMID:26236960

  6. 75 FR 3665 - Regulations under the Comprehensive Smokeless Tobacco Health Education Act; Termination of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-22

    ... cars, banners, flags, and other event-related objects. On November 4, 1993 (58 FR 58810), the FTC... Tobacco to Protect Children and Adolescents, 61 FR 44615-618 (Aug. 28, 1996). Those regulations...

  7. Ethylene acts as a negative regulator of glucose induced lateral root emergence in Arabidopsis

    PubMed Central

    Singh, Manjul; Gupta, Aditi; Laxmi, Ashverya

    2015-01-01

    Plants, being sessile organisms, are more exposed to the hazards of constantly changing environmental conditions globally. During the lifetime of a plant, the root system encounters various challenges such as obstacles, pathogens, high salinity, water logging, nutrient scarcity etc. The developmental plasticity of the root system provides brilliant adaptability to plants to counter the changes exerted by both external as well as internal cues and achieve an optimized growth status. Phytohormones are one of the major intrinsic factors regulating all aspects of plant growth and development both independently as well as through complex signal integrations at multiple levels. We have previously shown that glucose (Glc) and brassinosteroid (BR) signalings interact extensively to regulate lateral root (LR) development in Arabidopsis.1 Auxin efflux as well as influx and downstream signaling components are also involved in Glc-BR regulation of LR emergence. Here, we provide evidence for involvement of ethylene signaling machinery downstream to Glc and BR in regulation of LR emergence. PMID:26236960

  8. 75 FR 66699 - Lacey Act Implementation Plan; Definitions for Exempt and Regulated Articles

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-29

    ... published in the Federal Register (75 FR 46859-46861, Docket No. APHIS-2009-0018) a proposal to establish... Implementation Plan; Definitions for Exempt and Regulated Articles AGENCY: Animal and Plant Health...

  9. 77 FR 61467 - Privacy Act of 1974: Revision of Privacy Act System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-09

    ... comments are related to SOR 21 or SOR 36. FOR FURTHER INFORMATION CONTACT: Ingrid Ripley, Program Analyst, (202) 205-7538. SUPPLEMENTARY INFORMATION: SBA is amending its Privacy Act System of Records, notice of which was previously published at 74 FR 14890 (April 1, 2009) and 77 FR 15835-01 (March 16, 2012),...

  10. GP supervisors' experience in supporting self-regulated learning: a balancing act.

    PubMed

    Sagasser, Margaretha H; Kramer, Anneke W M; van Weel, Chris; van der Vleuten, Cees P M

    2015-08-01

    Self-regulated learning is essential for professional development and lifelong learning. As self-regulated learning has many inaccuracies, the need to support self-regulated learning has been recommended. Supervisors can provide such support. In a prior study trainees reported on the variation in received supervisor support. This study aims at exploring supervisors' perspectives. The aim is to explore how supervisors experience self-regulated learning of postgraduate general practitioners (GP) trainees and their role in this, and what helps and hinders them in supervising. In a qualitative study using a phenomenological approach, we interviewed 20 supervisors of first- and third-year postgraduate GP trainees. Supervisors recognised trainee activity in self-regulated learning and adapted their coaching style to trainee needs, occasionally causing conflicting emotions. Supervisors' beliefs regarding their role, trainees' role and the usefulness of educational interventions influenced their support. Supervisors experienced a relation between patient safety, self-regulated learning and trainee capability to learn. Supervisor training was helpful to exchange experience and obtain advice. Supervisors found colleagues helpful in sharing supervision tasks or in calibrating judgments of trainees. Busy practice occasionally hindered the supervisory process. In conclusion, supervisors adapt their coaching to trainees' self-regulated learning, sometimes causing conflicting emotions. Patient safety and entrustment are key aspects of the supervisory process. Supervisors' beliefs about their role and trainees' role influence their support. Supervisor training is important to increase awareness of these beliefs and the influence on their behaviour, and to improve the use of educational instruments. The results align with findings from other (medical) education, thereby illustrating its relevance. PMID:25314934

  11. Discrete complexes immobilized onto Click-SBA-15 silica: Controllable loadings and the impact of surface coverage on catalysis

    PubMed Central

    Nakazawa, Jun; Smith, Brian J.; Stack, T. Daniel P.

    2012-01-01

    Azidopropyl functionalized mesoporous silica SBA-15 were prepared with variable azide loadings of 0.03 – 0.7 mmol g−1 (ca. 2 – 50% of maximal surface coverage) through a direct synthesis, co-condensation approach. These materials are functionalized selectively with ethynylated organic moieties through the copper-catalyzed azide alkyne cycloaddition (CuAAC) or “click” reaction. Specific loading within a material can be regulated by either the azide loading or limiting the alkyne reagent relative to the azide loading. The immobilization of ferrocene, pyrene, tris(pyridylmethyl)amine (TPA), and iron porphyrin (FeTPP) demonstrates the robust nature and reproducibility of this two step synthetic attachment strategy. Loading-sensitive pyrene fluorescence correlates with a theoretically random. surface distribution, rather than a uniform one; full site-isolation of tethered moieties ca. 15 Å in length. occurs at loadings less than 0.02 mmol g−1. The effect of surface loading on reactivity is observed in oxygenation of SBA-15-[CuI(TPA)]. SBA-15-[MnII(TPA)]-catalyzed epoxidation exhibits a systematic dependence on surface loading. A comparison of homogeneous, site-isolated and site-dense complexes provides insight into catalyst speciation and ligand activity. PMID:22277027

  12. CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster

    PubMed Central

    Domanitskaya, Elena; Schüpbach, Trudi

    2012-01-01

    The Notch signaling pathway plays important roles in a variety of developmental events. The context-dependent activities of positive and negative modulators dramatically increase the diversity of cellular responses to Notch signaling. In a screen for mutations affecting the Drosophila melanogaster follicular epithelium, we isolated a mutation in CoREST that disrupts the Notch-dependent mitotic-to-endocycle switch of follicle cells at stage 6 of oogenesis. We show that Drosophila CoREST positively regulates Notch signaling, acting downstream of the proteolytic cleavage of Notch but upstream of Hindsight activity; the Hindsight gene is a Notch target that coordinates responses in the follicle cells. We show that CoREST genetically interacts with components of the Notch repressor complex, Hairless, C-terminal Binding Protein and Groucho. In addition, we demonstrate that levels of H3K27me3 and H4K16 acetylation are dramatically increased in CoREST mutant follicle cells. Our data indicate that CoREST acts as a positive modulator of the Notch pathway in the follicular epithelium as well as in wing tissue, and suggests a previously unidentified role for CoREST in the regulation of Notch signaling. Given its high degree of conservation among species, CoREST probably also functions as a regulator of Notch-dependent cellular events in other organisms. PMID:22331351

  13. Regulation by consensus: The expanded use of regulatory negotiation under the Clean Air Act

    SciTech Connect

    Claiborne, M.L.

    1996-12-31

    This article discusses the consensus building approach, which stems from the more formal regulatory negotiation process under the Negotiated Rulemaking Act of 1990, for improving air quality. The article uses as examples the joint plan to improve air quality and visibility in the Grand Canyon and 15 other national parks and wilderness areas in the SW USA, and the Southern Appalachian Mountain initiative tackling more complex issues including visibility, ground ozone, acid deposition, etc.

  14. Insulin/IGF-1 and Hypoxia Signaling Act in Concert to Regulate Iron Homeostasis in Caenorhabditis elegans

    PubMed Central

    Ackerman, Daniel; Gems, David

    2012-01-01

    Iron plays an essential role in many biological processes, but also catalyzes the formation of reactive oxygen species (ROS), which can cause molecular damage. Iron homeostasis is therefore a critical determinant of fitness. In Caenorhabditis elegans, insulin/IGF-1 signaling (IIS) promotes growth and reproduction but limits stress resistance and lifespan through inactivation of the DAF-16/FoxO transcription factor (TF). We report that long-lived daf-2 insulin/IGF-1 receptor mutants show a daf-16–dependent increase in expression of ftn-1, which encodes the iron storage protein H-ferritin. To better understand the regulation of iron homeostasis, we performed a TF–limited genetic screen for factors influencing ftn-1 gene expression. The screen identified the heat-shock TF hsf-1, the MAD bHLH TF mdl-1, and the putative histone acetyl transferase ada-2 as activators of ftn-1 expression. It also revealed that the HIFα homolog hif-1 and its binding partner aha-1 (HIFβ) are potent repressors of ftn-1 expression. ftn-1 expression is induced by exposure to iron, and we found that hif-1 was required for this induction. In addition, we found that the prolyl hydroxylase EGL-9, which represses HIF-1 via the von Hippel-Lindau tumor suppressor VHL-1, can also act antagonistically to VHL-1 in regulating ftn-1. This suggests a novel mechanism for HIF target gene regulation by these evolutionarily conserved and clinically important hydroxylases. Our findings imply that the IIS and HIF pathways act together to regulate iron homeostasis in C. elegans. We suggest that IIS/DAF-16 regulation of ftn-1 modulates a trade-off between growth and stress resistance, as elevated iron availability supports growth but also increases ROS production. PMID:22396654

  15. 32 CFR 644.511 - Certificate of competency by SBA.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 4 2013-07-01 2013-07-01 false Certificate of competency by SBA. 644.511 Section 644.511 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  16. 32 CFR 644.511 - Certificate of competency by SBA.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 4 2014-07-01 2013-07-01 true Certificate of competency by SBA. 644.511 Section 644.511 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  17. 32 CFR 644.511 - Certificate of competency by SBA.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 4 2011-07-01 2011-07-01 false Certificate of competency by SBA. 644.511 Section 644.511 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  18. 32 CFR 644.511 - Certificate of competency by SBA.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 4 2012-07-01 2011-07-01 true Certificate of competency by SBA. 644.511 Section 644.511 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  19. 13 CFR 107.509 - Employment of SBA officials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Employment of SBA officials. 107.509 Section 107.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee General Requirements § 107.509 Employment of...

  20. 13 CFR 107.560 - Subordination of SBA's creditor position.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Subordination of SBA's creditor position. 107.560 Section 107.560 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee Borrowing by Licensees from...

  1. 13 CFR 108.509 - Employment of SBA officials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Employment of SBA officials. 108.509 Section 108.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM Managing the Operations of a NMVC Company General Requirements §...

  2. 75 FR 13145 - SBA Lender Risk Rating System

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ... ADMINISTRATION SBA Lender Risk Rating System AGENCY: Small Business Administration. ACTION: Notice; extension of... comment period for the notice published on March 1, 2010 (75 FR 9257) is extended until May 30, 2010. FOR FURTHER INFORMATION CONTACT: Bryan Hooper, Director, Office of Credit Risk Management, U.S. Small...

  3. 13 CFR 121.103 - How does SBA determine affiliation?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... CFR 2510.3-101(d); (ii) Employee benefit or pension plans established and maintained by the Federal... Innovation Research (SBIR) and Small Business Technology Transfer (STTR) programs, the bases for affiliation... affiliation and any others set forth in § 121.702 apply for purposes of SBA's SBIR and STTR programs. (9)...

  4. 13 CFR 121.103 - How does SBA determine affiliation?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CFR 2510.3-101(d); (ii) Employee benefit or pension plans established and maintained by the Federal... his/her position in the concern, has a critical influence in or substantive control over the...) Two firms approved by SBA to be a mentor and protégé under 13 CFR 124.520 may joint venture as a...

  5. 13 CFR 107.509 - Employment of SBA officials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Employment of SBA officials. 107.509 Section 107.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee General Requirements § 107.509 Employment of...

  6. 13 CFR 108.509 - Employment of SBA officials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Employment of SBA officials. 108.509 Section 108.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM Managing the Operations of a NMVC Company General Requirements §...

  7. 13 CFR 108.509 - Employment of SBA officials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Employment of SBA officials. 108.509 Section 108.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM Managing the Operations of a NMVC Company General Requirements §...

  8. 13 CFR 107.560 - Subordination of SBA's creditor position.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Subordination of SBA's creditor position. 107.560 Section 107.560 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee Borrowing by Licensees from...

  9. 13 CFR 107.509 - Employment of SBA officials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Employment of SBA officials. 107.509 Section 107.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee General Requirements § 107.509 Employment of...

  10. 13 CFR 107.560 - Subordination of SBA's creditor position.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Subordination of SBA's creditor position. 107.560 Section 107.560 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee Borrowing by Licensees from...

  11. 13 CFR 107.560 - Subordination of SBA's creditor position.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Subordination of SBA's creditor position. 107.560 Section 107.560 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee Borrowing by Licensees from...

  12. 13 CFR 108.509 - Employment of SBA officials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Employment of SBA officials. 108.509 Section 108.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM Managing the Operations of a NMVC Company General Requirements §...

  13. 13 CFR 108.509 - Employment of SBA officials.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Employment of SBA officials. 108.509 Section 108.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM Managing the Operations of a NMVC Company General Requirements §...

  14. 13 CFR 107.509 - Employment of SBA officials.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Employment of SBA officials. 107.509 Section 107.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee General Requirements § 107.509 Employment of...

  15. 13 CFR 107.509 - Employment of SBA officials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Employment of SBA officials. 107.509 Section 107.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee General Requirements § 107.509 Employment of...

  16. 13 CFR 107.560 - Subordination of SBA's creditor position.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Subordination of SBA's creditor position. 107.560 Section 107.560 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Managing the Operations of a Licensee Borrowing by Licensees from...

  17. 75 FR 9257 - SBA Lender Risk Rating System

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-01

    ... found at 72 FR 27611, 27619 (May 16, 2007). B. Lender Risk Rating The SBA Lender risk rating (LRR) is a... Rating System (72 FR 25624). A final notice was published in the Federal Register on May 16, 2007 (72 FR... System Notice. 71 FR 25624 (May 1, 2006). The factors were run through the model in various...

  18. Nitric Oxide Acts as a Positive Regulator to Induce Metamorphosis of the Ascidian Herdmania momus

    PubMed Central

    Ueda, Nobuo; Degnan, Sandie M.

    2013-01-01

    Marine invertebrates commonly have a biphasic life cycle in which the metamorphic transition from a pelagic larva to a benthic post-larva is mediated by the nitric oxide signalling pathway. Nitric oxide (NO) is synthesised by nitric oxide synthase (NOS), which is a client protein of the molecular chaperon heat shock protein 90 (HSP90). It is notable, then, that both NO and HSP90 have been implicated in regulating metamorphosis in marine invertebrates as diverse as urochordates, echinoderms, molluscs, annelids, and crustaceans. Specifically, the suppression of NOS activity by the application of either NOS- or HSP90-inhibiting pharmacological agents has been shown consistently to induce the initiation of metamorphosis, leading to the hypothesis that a negative regulatory role of NO is widely conserved in biphasic life cycles. Further, the induction of metamorphosis by heat-shock has been demonstrated for multiple species. Here, we investigate the regulatory role of NO in induction of metamorphosis of the solitary tropical ascidian, Herdmania momus. By coupling pharmacological treatments with analysis of HmNOS and HmHSP90 gene expression, we present compelling evidence of a positive regulatory role for NO in metamorphosis of this species, in contrast to all existing ascidian data that supports the hypothesis of NO as a conserved negative regulator of metamorphosis. The exposure of competent H. momus larvae to a NOS inhibitor or an NO donor results in an up-regulation of NOS and HSP90 genes. Heat shock of competent larvae induces metamorphosis in a temperature dependent manner, up to a thermal tolerance that approaches 35°C. Both larval/post-larval survival and the appearance of abnormal morphologies in H. momus post-larvae reflect the magnitude of up-regulation of the HSP90 gene in response to heat-shock. The demonstrated role of NO as a positive metamorphic regulator in H. momus suggests the existence of inter-specific adaptations of NO regulation in ascidian

  19. Regulations for the administration and enforcement of the Radiation Control for Health and Safety Act of 1968

    SciTech Connect

    Not Available

    1988-04-01

    The purpose of the Act is to protect the public from unnecessary exposure to radiation from electronic products, including ionizing, nonionizing, electromagnetic, or particulate radiation, or any sonic, infrasonic, or ultrasonic wave. The regulations in the publication cover general provisions, statements of policy and interpretation, radiation protection recommendations, recordkeeping and reporting requirements for manufacturers, dealers, and distributors; notification requirements for defects or failure to comply; repurchase, repairs, or replacement of electronic products; importation of products; and performance standards for television receivers, cathode ray tubes, diagnostic x-ray equipment, radiographic equipment, fluoroscopic equipment, computer aided tomography equipment, microwave ovens, laser products, sunlamps, ultraviolet lamps, mercury lamps, and ultrasonic therapy products.

  20. Disemployment effects caused by regulation of drilling fluids and produced waters as hazardous under the Resource Conservation and Recovery Act

    SciTech Connect

    Flaim, S.J.

    1988-03-01

    This report reviews and compares several studies of the effects on employment of regulating wastes from oil and natural gas exploration and extraction under the Resource Conservation and Recovery Act (RCRA). The waste management scenarios on which most of the studies were based were developed by the U.S. Environmental Protection Agency. The analyses show that as many as 500,000-700,000 jobs may be lost in the first year if RCRA Subtitle C rules are applied to drilling fluids and produced waters. As a results, unemployment in major oil-producing states could rise by as much as six percentage points. 13 refs., 4 tabs.

  1. The Activity Centres (Young Persons' Safety) Act 1995, the Adventure Activities Licensing Regulations 1996.

    ERIC Educational Resources Information Center

    Journal of Adventure Education and Outdoor Leadership, 1996

    1996-01-01

    Explains licensing regulations applicable in the United Kingdom to commercial providers of adventure education programs that offer caving, climbing, trekking, and water sports; provides information on license applications, including licensing standards related to risk assessment and safety management procedures; and addresses issues related to the…

  2. 78 FR 59101 - Regulations Implementing the Byrd Amendments to the Black Lung Benefits Act: Determining Coal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-25

    ... public comment. 77 FR 19456-19478 (Mar. 30, 2012). These amendments reinstated two BLBA entitlement... Department recounted the history of these provisions in the NPRM. 77 FR at 19456-58. The Department also... streamlining the regulations where possible. See generally Executive Order 13563, 76 FR 3821 (January 18,...

  3. 78 FR 6621 - Ability To Repay Standards Under the Truth in Lending Act (Regulation Z)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... lending model, and ties to their communities enable them to make more accurate assessments of consumers... deterioration in underwriting standards. See 73 FR 44524 (Jul. 30, 2008). The following is presented as..., which substantially parallels the Board's Regulation Z, 12 CFR part 226. See 76 FR 79767 (Dec. 22,...

  4. 75 FR 68911 - Regulations Under the Genetic Information Nondiscrimination Act of 2008

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-09

    ... document. See 74 FR 9056 (March 2, 2009). Several days earlier, on February 25, 2009, the Commission held a... FR 51664. The HHS Office for Civil Rights is responsible for issuing the regulations applicable to GINA section 105 and issued a proposed rule on October 7, 2009 at 74 FR 51698. Among the various...

  5. 77 FR 19455 - Regulations Implementing the Byrd Amendments to the Black Lung Benefits Act: Determining Coal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ..., 20 CFR 718.2 (2011); 45 FR 13677, 13679 (Feb. 29, 1980). The proposed rule does, however, retain... 65 FR 79920, 80029 (Dec. 20, 2000); 20 CFR 725.4(d) (2011). Consequently, there is no reason to... Federal Regulations in 2000. See 65 FR 79920, 80029 (Dec. 20, 2000); 20 CFR 725.4(d) (2011)....

  6. 77 FR 23196 - Native American Graves Protection and Repatriation Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ... amendments to the regulation concerning: Civil penalties (68 FR 16354, April 3, 2003); Future applicability (72 FR 13189, March 21, 2007); and Disposition of culturally unidentifiable human remains (75 FR 12378... Department of the Interior (Department) published the initial rules to implement NAGPRA (60 FR...

  7. 78 FR 27078 - Native American Graves Protection and Repatriation Act Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... Government Relations with Native American Tribal Governments'' (59 FR 22951, April 29, 1994); Executive Order... Office of the Secretary of the Interior 43 CFR Part 10 RIN 1024-AD99 Native American Graves Protection.... SUMMARY: This final rule revises regulations implementing the Native American Graves Protection...

  8. 77 FR 234 - Rules and Regulations Under the Textile Fiber Products Identification Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-04

    .... SUPPLEMENTARY INFORMATION: On November 7, 2011, the Commission published its ANPR (76 FR 68690) seeking comment...; ] FEDERAL TRADE COMMISSION 16 CFR Part 303 Rules and Regulations Under the Textile Fiber Products... section below. Write ``Textile Rules, 16 CFR Part 303, Project No. P948404'' on your comment, and...

  9. 78 FR 60686 - Regulations Implementing the Byrd Amendments to the Black Lung Benefits Act: Determining Coal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    ... of Workers' Compensation Programs 20 CFR Parts 718 and 725 RIN 1240-AA04 Regulations Implementing the... Benefits; Correction AGENCY: Office of Workers' Compensation Programs, Labor. ACTION: Final rule; correction. SUMMARY: The Office of Workers' Compensation Programs is correcting the preamble to a final...

  10. Strigolactone acts downstream of auxin to regulate bud outgrowth in pea and Arabidopsis.

    PubMed

    Brewer, Philip B; Dun, Elizabeth A; Ferguson, Brett J; Rameau, Catherine; Beveridge, Christine A

    2009-05-01

    During the last century, two key hypotheses have been proposed to explain apical dominance in plants: auxin promotes the production of a second messenger that moves up into buds to repress their outgrowth, and auxin saturation in the stem inhibits auxin transport from buds, thereby inhibiting bud outgrowth. The recent discovery of strigolactone as the novel shoot-branching inhibitor allowed us to test its mode of action in relation to these hypotheses. We found that exogenously applied strigolactone inhibited bud outgrowth in pea (Pisum sativum) even when auxin was depleted after decapitation. We also found that strigolactone application reduced branching in Arabidopsis (Arabidopsis thaliana) auxin response mutants, suggesting that auxin may act through strigolactones to facilitate apical dominance. Moreover, strigolactone application to tiny buds of mutant or decapitated pea plants rapidly stopped outgrowth, in contrast to applying N-1-naphthylphthalamic acid (NPA), an auxin transport inhibitor, which significantly slowed growth only after several days. Whereas strigolactone or NPA applied to growing buds reduced bud length, only NPA blocked auxin transport in the bud. Wild-type and strigolactone biosynthesis mutant pea and Arabidopsis shoots were capable of instantly transporting additional amounts of auxin in excess of endogenous levels, contrary to predictions of auxin transport models. These data suggest that strigolactone does not act primarily by affecting auxin transport from buds. Rather, the primary repressor of bud outgrowth appears to be the auxin-dependent production of strigolactones. PMID:19321710

  11. Thyroid Hormone May Regulate mRNA Abundance in Liver by Acting on MicroRNAs

    PubMed Central

    Dong, Hongyan; Paquette, Martin; Williams, Andrew; Zoeller, R. Thomas; Wade, Mike; Yauk, Carole

    2010-01-01

    MicroRNAs (miRNAs) are extensively involved in diverse biological processes. However, very little is known about the role of miRNAs in mediating the action of thyroid hormones (TH). Appropriate TH levels are known to be critically important for development, differentiation and maintenance of metabolic balance in mammals. We induced transient hypothyroidism in juvenile mice by short-term exposure to methimazole and perchlorate from post natal day (PND) 12 to 15. The expression of miRNAs in the liver was analyzed using Taqman Low Density Arrays (containing up to 600 rodent miRNAs). We found the expression of 40 miRNAs was significantly altered in the livers of hypothyroid mice compared to euthyroid controls. Among the miRNAs, miRs-1, 206, 133a and 133b exhibited a massive increase in expression (50- to 500-fold). The regulation of TH on the expression of miRs-1, 206, 133a and 133b was confirmed in various mouse models including: chronic hypothyroid, short-term hyperthyroid and short-term hypothyroid followed by TH supplementation. TH regulation of these miRNAs was also confirmed in mouse hepatocyte AML 12 cells. The expression of precursors of miRs-1, 206, 133a and 133b were examined in AML 12 cells and shown to decrease after TH treatment, only pre-mir-206 and pre-mir-133b reached statistical significance. To identify the targets of these miRNAs, DNA microarrays were used to examine hepatic mRNA levels in the short-term hypothyroid mouse model relative to controls. We found transcripts from 92 known genes were significantly altered in these hypothyroid mice. Web-based target predication software (TargetScan and Microcosm) identified 14 of these transcripts as targets of miRs-1, 206, 133a and 133b. The vast majority of these mRNA targets were significantly down-regulated in hypothyroid mice, corresponding with the up-regulation of miRs-1, 206, 133a and 133b in hypothyroid mouse liver. To further investigate target genes, miR-206 was over-expressed in AML 12 cells. TH

  12. Potential Impact of Clean Air Act Regulations on Nitrogen Fate and Transport in the Neuse River Basin: a Modeling Investigation Using CMAQ and SWAT

    EPA Science Inventory

    There has been extensive analysis of Clean Air Act Amendment (CAAA) regulation impacts to changes in atmospheric nitrogen deposition; however, few studies have focused on watershed nitrogen transfer particularly regarding long-term predictions. In this study, we investigated impa...

  13. Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance

    PubMed Central

    Tsang, Chi Kwan; Liu, Yuan; Thomas, Janice; Zhang, Yanjie; Zheng, X. F. Steven

    2015-01-01

    Summary Superoxide dismutase 1 (Sod1) has been known for nearly half a century for catalysis of superoxide to hydrogen peroxide. Here we report a new Sod1 function in oxidative signaling: in response to elevated endogenous and exogenous reactive oxygen species (ROS), Sod1 rapidly relocates into the nucleus, which is important for maintaining genomic stability. Interestingly, H2O2 is sufficient to promote Sod1 nuclear localization, indicating that it is responding to general ROS rather than Sod1 substrate superoxide. ROS signaling is mediated by Mec1/ATM and its effector Dun1/Cds1 kinase, through Dun1 interaction with Sod1 and regulation of Sod1 by phosphorylation at S60, 99. In the nucleus, Sod1 binds to the promoters and regulates the expression of oxidative resistance and repair genes. Altogether, our study unravels an unorthodox function of Sod1 as a transcription factor and elucidates the regulatory mechanism for its localization. PMID:24647101

  14. Anaplastic Lymphoma Kinase Acts in the Drosophila Mushroom Body to Negatively Regulate Sleep

    PubMed Central

    Bai, Lei; Sehgal, Amita

    2015-01-01

    Though evidence is mounting that a major function of sleep is to maintain brain plasticity and consolidate memory, little is known about the molecular pathways by which learning and sleep processes intercept. Anaplastic lymphoma kinase (Alk), the gene encoding a tyrosine receptor kinase whose inadvertent activation is the cause of many cancers, is implicated in synapse formation and cognitive functions. In particular, Alk genetically interacts with Neurofibromatosis 1 (Nf1) to regulate growth and associative learning in flies. We show that Alk mutants have increased sleep. Using a targeted RNAi screen we localized the negative effects of Alk on sleep to the mushroom body, a structure important for both sleep and memory. We also report that mutations in Nf1 produce a sexually dimorphic short sleep phenotype, and suppress the long sleep phenotype of Alk. Thus Alk and Nf1 interact in both learning and sleep regulation, highlighting a common pathway in these two processes. PMID:26536237

  15. Lysophosphatidic acid acts as a nutrient-derived developmental cue to regulate early hematopoiesis

    PubMed Central

    Li, Haisen; Yue, Rui; Wei, Bin; Gao, Ge; Du, Jiulin; Pei, Gang

    2014-01-01

    Primitive hematopoiesis occurs in the yolk sac blood islands during vertebrate embryogenesis, where abundant phosphatidylcholines (PC) are available as important nutrients for the developing embryo. However, whether these phospholipids also generate developmental cues to promote hematopoiesis is largely unknown. Here, we show that lysophosphatidic acid (LPA), a signaling molecule derived from PC, regulated hemangioblast formation and primitive hematopoiesis. Pharmacological and genetic blockage of LPA receptor 1 (LPAR1) or autotoxin (ATX), a secretory lysophospholipase that catalyzes LPA production, inhibited hematopoietic differentiation of mouse embryonic stem cells and impaired the formation of hemangioblasts. Mechanistic experiments revealed that the regulatory effect of ATX-LPA signaling was mediated by PI3K/Akt-Smad pathway. Furthermore, during in vivo embryogenesis in zebrafish, LPA functioned as a developmental cue for hemangioblast formation and primitive hematopoiesis. Taken together, we identified LPA as an important nutrient-derived developmental cue for primitive hematopoiesis as well as a novel mechanism of hemangioblast regulation. PMID:24829209

  16. Two Group A Streptococcal Peptide Pheromones Act through Opposing Rgg Regulators to Control Biofilm Development

    PubMed Central

    Chang, Jennifer C.; LaSarre, Breah; Jimenez, Juan C.; Aggarwal, Chaitanya; Federle, Michael J.

    2011-01-01

    Streptococcus pyogenes (Group A Streptococcus, GAS) is an important human commensal that occasionally causes localized infections and less frequently causes severe invasive disease with high mortality rates. How GAS regulates expression of factors used to colonize the host and avoid immune responses remains poorly understood. Intercellular communication is an important means by which bacteria coordinate gene expression to defend against host assaults and competing bacteria, yet no conserved cell-to-cell signaling system has been elucidated in GAS. Encoded within the GAS genome are four rgg-like genes, two of which (rgg2 and rgg3) have no previously described function. We tested the hypothesis that rgg2 or rgg3 rely on extracellular peptides to control target-gene regulation. We found that Rgg2 and Rgg3 together tightly regulate two linked genes encoding new peptide pheromones. Rgg2 activates transcription of and is required for full induction of the pheromone genes, while Rgg3 plays an antagonistic role and represses pheromone expression. The active pheromone signals, termed SHP2 and SHP3, are short and hydrophobic (DI[I/L]IIVGG), and, though highly similar in sequence, their ability to disrupt Rgg3-DNA complexes were observed to be different, indicating that specificity and differential activation of promoters are characteristics of the Rgg2/3 regulatory circuit. SHP-pheromone signaling requires an intact oligopeptide permease (opp) and a metalloprotease (eep), supporting the model that pro-peptides are secreted, processed to the mature form, and subsequently imported to the cytoplasm to interact directly with the Rgg receptors. At least one consequence of pheromone stimulation of the Rgg2/3 pathway is increased biogenesis of biofilms, which counteracts negative regulation of biofilms by RopB (Rgg1). These data provide the first demonstration that Rgg-dependent quorum sensing functions in GAS and substantiate the role that Rggs play as peptide receptors across the

  17. 75 FR 60263 - Federal Acquisition Regulation; Offering a Construction Requirement-8(a) Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ... Federal Acquisition Regulation; Offering a Construction Requirement--8(a) Program AGENCIES: Department of... submitting offering letters to SBA for a construction requirement for which a specific offeror is nominated... offerings for construction ] requirements be submitted to the SBA District Office for the geographical...

  18. Phosphorylation acts positively and negatively to regulate MRTF-A subcellular localisation and activity.

    PubMed

    Panayiotou, Richard; Miralles, Francesc; Pawlowski, Rafal; Diring, Jessica; Flynn, Helen R; Skehel, Mark; Treisman, Richard

    2016-01-01

    The myocardin-related transcription factors (MRTF-A and MRTF-B) regulate cytoskeletal genes through their partner transcription factor SRF. The MRTFs bind G-actin, and signal-regulated changes in cellular G-actin concentration control their nuclear accumulation. The MRTFs also undergo Rho- and ERK-dependent phosphorylation, but the function of MRTF phosphorylation, and the elements and signals involved in MRTF-A nuclear export are largely unexplored. We show that Rho-dependent MRTF-A phosphorylation reflects relief from an inhibitory function of nuclear actin. We map multiple sites of serum-induced phosphorylation, most of which are S/T-P motifs and show that S/T-P phosphorylation is required for transcriptional activation. ERK-mediated S98 phosphorylation inhibits assembly of G-actin complexes on the MRTF-A regulatory RPEL domain, promoting nuclear import. In contrast, S33 phosphorylation potentiates the activity of an autonomous Crm1-dependent N-terminal NES, which cooperates with five other NES elements to exclude MRTF-A from the nucleus. Phosphorylation thus plays positive and negative roles in the regulation of MRTF-A. PMID:27304076

  19. Phosphorylation acts positively and negatively to regulate MRTF-A subcellular localisation and activity

    PubMed Central

    Panayiotou, Richard; Miralles, Francesc; Pawlowski, Rafal; Diring, Jessica; Flynn, Helen R; Skehel, Mark; Treisman, Richard

    2016-01-01

    The myocardin-related transcription factors (MRTF-A and MRTF-B) regulate cytoskeletal genes through their partner transcription factor SRF. The MRTFs bind G-actin, and signal-regulated changes in cellular G-actin concentration control their nuclear accumulation. The MRTFs also undergo Rho- and ERK-dependent phosphorylation, but the function of MRTF phosphorylation, and the elements and signals involved in MRTF-A nuclear export are largely unexplored. We show that Rho-dependent MRTF-A phosphorylation reflects relief from an inhibitory function of nuclear actin. We map multiple sites of serum-induced phosphorylation, most of which are S/T-P motifs and show that S/T-P phosphorylation is required for transcriptional activation. ERK-mediated S98 phosphorylation inhibits assembly of G-actin complexes on the MRTF-A regulatory RPEL domain, promoting nuclear import. In contrast, S33 phosphorylation potentiates the activity of an autonomous Crm1-dependent N-terminal NES, which cooperates with five other NES elements to exclude MRTF-A from the nucleus. Phosphorylation thus plays positive and negative roles in the regulation of MRTF-A. DOI: http://dx.doi.org/10.7554/eLife.15460.001 PMID:27304076

  20. Identification of cis- and trans-acting elements regulating calretinin expression in mesothelioma cells

    PubMed Central

    Kresoja-Rakic, Jelena; Kapaklikaya, Esra; Ziltener, Gabriela; Dalcher, Damian; Santoro, Raffaella; Christensen, Brock C.; Johnson, Kevin C.; Schwaller, Beat; Weder, Walter; Stahel, Rolf A.; Felley-Bosco, Emanuela

    2016-01-01

    Calretinin (CALB2) is a diagnostic marker for epithelioid mesothelioma. It is also a prognostic marker since patients with tumors expressing high calretinin levels have better overall survival. Silencing of calretinin decreases viability of epithelioid mesothelioma cells. Our aim was to elucidate mechanisms regulating calretinin expression in mesothelioma. Analysis of calretinin transcript and protein suggested a control at the mRNA level. Treatment with 5-aza-2′-deoxycytidine and analysis of TCGA data indicated that promoter methylation is not likely to be involved. Therefore, we investigated CALB2 promoter by analyzing ~1kb of genomic sequence surrounding the transcription start site (TSS) + 1 using promoter reporter assay. Deletion analysis of CALB2 proximal promoter showed that sequence spanning the −161/+80bp region sustained transcriptional activity. Site-directed analysis identified important cis-regulatory elements within this −161/+80bp CALB2 promoter. EMSA and ChIP assays confirmed binding of NRF-1 and E2F2 to the CALB2 promoter and siRNA knockdown of NRF-1 led to decreased expression of calretinin. Cell synchronization experiment showed that calretinin expression was cell cycle regulated with a peak of expression at G1/S phase. This study provides the first insight in the regulation of CALB2 expression in mesothelioma cells. PMID:26848772

  1. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act...

  2. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act...

  3. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act...

  4. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act...

  5. 25 CFR 309.1 - How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false How do the regulations in this part carry out the Indian Arts and Crafts Act of 1990? 309.1 Section 309.1 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.1 How do the regulations in this part carry out the Indian Arts and Crafts Act...

  6. Functional overlap in the cis-acting regulation of the V(D)J recombination at the TCRβ locus

    PubMed Central

    Khor, Bernard; Mahowald, Grace; Khor, Katrina; Sleckman, Barry P.

    2009-01-01

    The second exon of lymphocyte antigen receptor genes is assembled in developing lymphocytes from component V, J and, in some cases, D gene segments through the process of V(D)J recombination. This process is initiated by an endonuclease comprised of the Rag-1 and Rag-2 proteins, collectively referred to as Rag. Rag binds to recombination signals (RSs) and catalyzes the pair-wise introduction of DNA double strand breaks (DSBs) at recombining gene segments. DNA cleavage by Rag is restricted both by intrinsic features of RSs, as well as the activity of other cis-acting elements, such as promoters and enhancers that regulate the accessibility of gene segments to Rag. In the TCRβ locus, accessibility of the Dβ1-Jβ1 gene segment cluster relies on the function of an enhancer, Eβ, and a promoter, PDβ1. Here we demonstrate that deletion of a small genomic region containing 5 of the 6 Jβ1 gene segments, but no known transcriptional regulatory elements, leads to a marked decrease in transcription and rearrangements involving the Dβ1 and Jβ1.1 gene segments. Surprisingly, point mutations in the RS of the Jβ1.1 gene segment not only impact Rag cleavage, but also lead to diminished transcription through the Dβ1-Jβ1 gene segment cluster. Our findings demonstrate that cis-acting elements that regulate transcription and accessibility of the TCRβ locus may functionally overlap with RS sequences, which are known primarily to direct Rag-mediated cleavage. PMID:19070901

  7. An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development.

    PubMed

    Chae, Eunyoung; Tan, Queenie K-G; Hill, Theresa A; Irish, Vivian F

    2008-04-01

    Plants flower in response to both environmental and endogenous signals. The Arabidopsis LEAFY (LFY) transcription factor is crucial in integrating these signals, and acts in part by activating the expression of multiple floral homeotic genes. LFY-dependent activation of the homeotic APETALA3 (AP3) gene requires the activity of UNUSUAL FLORAL ORGANS (UFO), an F-box component of an SCF ubiquitin ligase, yet how this regulation is effected has remained unclear. Here, we show that UFO physically interacts with LFY both in vitro and in vivo, and this interaction is necessary to recruit UFO to the AP3 promoter. Furthermore, a transcriptional repressor domain fused to UFO reduces endogenous LFY activity in plants, supporting the idea that UFO acts as part of a transcriptional complex at the AP3 promoter. Moreover, chemical or genetic disruption of proteasome activity compromises LFY-dependent AP3 activation, indicating that protein degradation is required to promote LFY activity. These results define an unexpected role for an F-box protein in functioning as a DNA-associated transcriptional co-factor in regulating floral homeotic gene expression. These results suggest a novel mechanism for promoting flower development via protein degradation and concomitant activation of the LFY transcription factor. This mechanism may be widely conserved, as homologs of UFO and LFY have been identified in a wide array of plant species. PMID:18287201

  8. 17 CFR 239.500 - Form D, notice of sales of securities under Regulation D and section 4(6) of the Securities Act...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Form D, notice of sales of securities under Regulation D and section 4(6) of the Securities Act of 1933. 239.500 Section 239.500 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION FORMS PRESCRIBED UNDER THE SECURITIES ACT OF 1933 Forms Pertaining to...

  9. The Texas space flight liability act and efficient regulation for the private commercial space flight era

    NASA Astrophysics Data System (ADS)

    Johnson, Christopher D.

    2013-12-01

    In the spring of 2011, the American state of Texas passed into law an act limiting the liability of commercial space flight entities. Under it, those companies would not be liable for space flight participant injuries, except in cases of intentional injury or injury proximately caused by the company's gross negligence. An analysis within the framework of international and national space law, but especially informed by the academic discipline of law and economics, discusses the incentives of all relevant parties and attempts to understand whether the law is economically "efficient" (allocating resources so as to yield maximum utility), and suited to further the development of the fledgling commercial suborbital tourism industry. Insights into the Texas law are applicable to other states hoping to foster commercial space tourism and considering space tourism related legislation.

  10. Polyadenylation in Rice Tungro Bacilliform Virus: cis-Acting Signals and Regulation

    PubMed Central

    Rothnie, Helen M.; Chen, Gang; Fütterer, Johannes; Hohn, Thomas

    2001-01-01

    The polyadenylation signal of rice tungro bacilliform virus (RTBV) was characterized by mutational and deletion analysis. The cis-acting signals required to direct polyadenylation conformed to what is known for plant poly(A) signals in general and were very similar to those of the related cauliflower mosaic virus. Processing was directed by a canonical AAUAAA poly(A) signal, an upstream UG-rich region considerably enhanced processing efficiency, and sequences downstream of the cleavage site were not required. When present at the end of a transcription unit, the cis-acting signals for 3′-end processing were highly efficient in both monocot (rice) and dicot (Nicotiana plumbaginifolia) protoplasts. In a promoter-proximal position, as in the viral genome, the signal was also efficiently processed in rice protoplasts, giving rise to an abundant “short-stop” (SS-) RNA. The proportion of SS-RNA was considerably lower in N. plumbaginifolia protoplasts. In infected plants, SS-RNA was hardly detectable, suggesting either that SS-RNA is unstable in infected plants or that read-through of the promoter-proximal poly(A) site is very efficient. SS-RNA is readily detectable in transgenic rice plants (A. Klöti, C. Henrich, S. Bieri, X. He, G. Chen, P. K. Burkhardt, J. Wünn, P. Lucca, T. Hohn, I. Potrylus, and J. Fütterer, 1999. Plant Mol. Biol. 40:249–266), thus the absence of SS-RNA in infected plants can be attributed to poly(A) site bypass in the viral context to ensure production of the full-length pregenomic viral RNA. RTBV poly(A) site suppression thus depends both on context and the expression system; our results suggest that the circular viral minichromosome directs assembly of a transcription-processing complex with specific properties to effect read-through of the promoter-proximal poly(A) signal. PMID:11287568

  11. Sclerostin Is a Locally Acting Regulator of Late-Osteoblast/Preosteocyte Differentiation and Regulates Mineralization Through a MEPE-ASARM-Dependent Mechanism

    PubMed Central

    Atkins, Gerald J; Rowe, Peter S; Lim, Hui P; Welldon, Katie J; Ormsby, Renee; Wijenayaka, Asiri R; Zelenchuk, Lesya; Evdokiou, Andreas; Findlay, David M

    2012-01-01

    The identity of the cell type responsive to sclerostin, a negative regulator of bone mass, is unknown. Since sclerostin is expressed in vivo by mineral-embedded osteocytes, we tested the hypothesis that sclerostin would regulate the behavior of cells actively involved in mineralization in adult bone, the preosteocyte. Differentiating cultures of human primary osteoblasts exposed to recombinant human sclerostin (rhSCL) for 35 days displayed dose- and time-dependent inhibition of in vitro mineralization, with late cultures being most responsive in terms of mineralization and gene expression. Treatment of advanced (day 35) cultures with rhSCL markedly increased the expression of the preosteocyte marker E11 and decreased the expression of mature markers DMP1 and SOST. Concomitantly, matrix extracellular phosphoglycoprotein (MEPE) expression was increased by rhSCL at both the mRNA and protein levels, whereas PHEX was decreased, implying regulation through the MEPE-ASARM axis. We confirmed that mineralization by human osteoblasts is exquisitely sensitive to the triphosphorylated ASARM-PO4 peptide. Immunostaining revealed that rhSCL increased the endogenous levels of MEPE-ASARM. Importantly, antibody-mediated neutralization of endogenous MEPE-ASARM antagonized the effect of rhSCL on mineralization, as did the PHEX synthetic peptide SPR4. Finally, we found elevated Sost mRNA expression in the long bones of HYP mice, suggesting that sclerostin may drive the increased MEPE-ASARM levels and mineralization defect in this genotype. Our results suggest that sclerostin acts through regulation of the PHEX/MEPE axis at the preosteocyte stage and serves as a master regulator of physiologic bone mineralization, consistent with its localization in vivo and its established role in the inhibition of bone formation. PMID:21312267

  12. Cyclic AMP Receptor Protein Acts as a Transcription Regulator in Response to Stresses in Deinococcus radiodurans

    PubMed Central

    Wang, Jiali; Liu, Chengzhi; Lu, Huizhi; Liu, Mengjia; Zhao, Ye; Tian, Bing; Wang, Liangyan; Hua, Yuejin

    2016-01-01

    The cyclic AMP receptor protein family of transcription factors regulates various metabolic pathways in bacteria, and also play roles in response to environmental changes. Here, we identify four homologs of the CRP family in Deinococcus radiodurans, one of which tolerates extremely high levels of oxidative stress and DNA-damaging reagents. Transcriptional levels of CRP were increased under hydrogen peroxide (H2O2) treatment during the stationary growth phase, indicating that CRPs function in response to oxidative stress. By constructing all CRP single knockout mutants, we found that the dr0997 mutant showed the lowest tolerance toward H2O2, ultraviolet radiation, ionizing radiation, and mitomycin C, while the phenotypes of the dr2362, dr0834, and dr1646 mutants showed slight or no significant differences from those of the wild-type strain. Taking advantage of the conservation of the CRP-binding site in many bacteria, we found that transcription of 18 genes, including genes encoding chromosome-partitioning protein (dr0998), Lon proteases (dr0349 and dr1974), NADH-quinone oxidoreductase (dr1506), thiosulfate sulfurtransferase (dr2531), the DNA repair protein UvsE (dr1819), PprA (dra0346), and RecN (dr1447), are directly regulated by DR0997. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses showed that certain genes involved in anti-oxidative responses, DNA repair, and various cellular pathways are transcriptionally attenuated in the dr0997 mutant. Interestingly, DR0997 also regulate the transcriptional levels of all CRP genes in this bacterium. These data suggest that DR0997 contributes to the extreme stress resistance of D. radiodurans via its regulatory role in multiple cellular pathways, such as anti-oxidation and DNA repair pathways. PMID:27182600

  13. CHD1 acts via the Hmgpi pathway to regulate mouse early embryogenesis.

    PubMed

    Suzuki, Shinnosuke; Nozawa, Yusuke; Tsukamoto, Satoshi; Kaneko, Takehito; Manabe, Ichiro; Imai, Hiroshi; Minami, Naojiro

    2015-07-01

    The protein CHD1 is a member of the family of ATPase-dependent chromatin remodeling factors. CHD1, which recognizes trimethylated histone H3 lysine 4, has been implicated in transcriptional activation in organisms ranging from yeast to humans. It is required for pre-mRNA maturation, maintenance of mouse embryonic stem cell pluripotency and rapid growth of the mouse epiblast. However, the function(s) of CHD1 in mouse preimplantation embryos has not yet been examined. Here, we show that loss of CHD1 function led to embryonic lethality after implantation. In mouse embryos in which Chd1 was targeted by siRNA microinjection, the expression of the key regulators of cell fate specification Pou5f1 (also known as Oct4), Nanog and Cdx2 was dramatically decreased, starting at mid-preimplantation gene activation (MGA). Moreover, expression of Hmgpi and Klf5, which regulate Pou5f1, Nanog and Cdx2, was also significantly suppressed at zygotic gene activation (ZGA). Suppression of Hmgpi expression in Chd1-knockdown embryos continued until the blastocyst stage, whereas suppression of Klf5 expression was relieved by the morula stage. Next, we rescued HMGPI expression via Hmgpi mRNA microinjection in Chd1-knockdown embryos. Consequently, Pou5f1, Nanog and Cdx2 expression was restored at MGA and live offspring were recovered. These findings indicate that CHD1 plays important roles in mouse early embryogenesis via activation of Hmgpi at ZGA. PMID:26092847

  14. Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis.

    PubMed

    Shi, Bihai; Zhang, Cui; Tian, Caihuan; Wang, Jin; Wang, Quan; Xu, Tengfei; Xu, Yan; Ohno, Carolyn; Sablowski, Robert; Heisler, Marcus G; Theres, Klaus; Wang, Ying; Jiao, Yuling

    2016-07-01

    Shoot branching requires the establishment of new meristems harboring stem cells; this phenomenon raises questions about the precise regulation of meristematic fate. In seed plants, these new meristems initiate in leaf axils to enable lateral shoot branching. Using live-cell imaging of leaf axil cells, we show that the initiation of axillary meristems requires a meristematic cell population continuously expressing the meristem marker SHOOT MERISTEMLESS (STM). The maintenance of STM expression depends on the leaf axil auxin minimum. Ectopic expression of STM is insufficient to activate axillary buds formation from plants that have lost leaf axil STM expressing cells. This suggests that some cells undergo irreversible commitment to a developmental fate. In more mature leaves, REVOLUTA (REV) directly up-regulates STM expression in leaf axil meristematic cells, but not in differentiated cells, to establish axillary meristems. Cell type-specific binding of REV to the STM region correlates with epigenetic modifications. Our data favor a threshold model for axillary meristem initiation, in which low levels of STM maintain meristematic competence and high levels of STM lead to meristem initiation. PMID:27398935

  15. Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis

    PubMed Central

    Tian, Caihuan; Wang, Jin; Xu, Tengfei; Xu, Yan; Ohno, Carolyn; Sablowski, Robert; Heisler, Marcus G.; Theres, Klaus; Wang, Ying

    2016-01-01

    Shoot branching requires the establishment of new meristems harboring stem cells; this phenomenon raises questions about the precise regulation of meristematic fate. In seed plants, these new meristems initiate in leaf axils to enable lateral shoot branching. Using live-cell imaging of leaf axil cells, we show that the initiation of axillary meristems requires a meristematic cell population continuously expressing the meristem marker SHOOT MERISTEMLESS (STM). The maintenance of STM expression depends on the leaf axil auxin minimum. Ectopic expression of STM is insufficient to activate axillary buds formation from plants that have lost leaf axil STM expressing cells. This suggests that some cells undergo irreversible commitment to a developmental fate. In more mature leaves, REVOLUTA (REV) directly up-regulates STM expression in leaf axil meristematic cells, but not in differentiated cells, to establish axillary meristems. Cell type-specific binding of REV to the STM region correlates with epigenetic modifications. Our data favor a threshold model for axillary meristem initiation, in which low levels of STM maintain meristematic competence and high levels of STM lead to meristem initiation. PMID:27398935

  16. Tmem178 acts in a novel negative feedback loop targeting NFATc1 to regulate bone mass

    PubMed Central

    Decker, Corinne E.; Yang, Zhengfeng; Rimer, Ryan; Park-Min, Kyung-Hyun; Macaubas, Claudia; Mellins, Elizabeth D.; Novack, Deborah V.; Faccio, Roberta

    2015-01-01

    Phospholipase C gamma-2 (PLCγ2)-dependent calcium (Ca2+) oscillations are indispensable for nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) activation and downstream gene transcription driving osteoclastogenesis during skeletal remodeling and pathological bone loss. Here we describe, to our knowledge, the first known function of transmembrane protein 178 (Tmem178), a PLCγ2 downstream target gene, as a critical modulator of the NFATc1 axis. In surprising contrast to the osteopetrotic phenotype of PLCγ2−/− mice, Tmem178−/− mice are osteopenic in basal conditions and are more susceptible to inflammatory bone loss, owing to enhanced osteoclast formation. Mechanistically, Tmem178 localizes to the ER membrane and regulates RANKL-induced Ca2+ fluxes, thus controlling NFATc1 induction. Importantly, down-regulation of Tmem178 is observed in human CD14+ monocytes exposed to plasma from systemic juvenile idiopathic arthritis patients. Similar to the mouse model, reduced Tmem178 expression in human cells correlates with excessive osteoclastogenesis. In sum, these findings identify an essential role for Tmem178 to maintain skeletal mass and limit pathological bone loss. PMID:26644563

  17. Modified SBA-15 as the carrier for metoprolol and papaverine: Adsorption and release study

    SciTech Connect

    Moritz, MichaL; Laniecki, Marek

    2011-07-15

    A series of modified SBA-15 materials were applied in drug delivery systems. The internal surface of siliceous hexagonal structure of SBA-15 was modified with different amount of (3-mercaptopropyl)trimethoxysilane (MPTMS) and oxidized in the presence of hydrogen peroxide. The sulfonated material was loaded with metoprolol tartrate or papaverine hydrochloride. Both drugs indicated strong chemical interaction with modified mesoporous surface. The characteristic of the obtained materials was performed with XRD and DRUV-vis spectrometry, themogravimetry and nitrogen adsorption (BET) measurements. The obtained results show that modification of the mesoporous materials leads towards significant decrease of the drug delivery rate. - Graphical abstract: XRD and DSC of the -SO{sub 3}H modified SBA-15 loaded with metoprolol. Highlights: > Modification of SBA-15 internal channels with SO{sub 3}H groups. > Adsorption of metoprolol and papaverine on modified SBA-15. > Uniform and homogeneous distribution of the drugs inside the mesoporous structure of SBA-15. > Release of the supported drugs.

  18. Nucleoside diphosphate regulation of overall rates of protein biosynthesis acting at the level of initiation.

    PubMed

    Hucul, J A; Henshaw, E C; Young, D A

    1985-12-15

    A sensitive assay method developed to examine the effects of subtle, physiologically relevant, changes in the levels of adenine and guanine mono-, di-, and triphosphorylated nucleotides specifically on the initiation of protein synthesis is described. Initiation rates are quantified by measuring the amount of protein synthesis resulting from the run-off of ribosomes which have initiated during defined intervals in a modified in vitro protein-synthesizing system developed from Ehrlich ascites tumor cell lysates (Henshaw, E.C., and Panniers, R. (1983) Methods Enzymol. 101, 616-629). The modifications include the attenuation of the ATP-regenerating system so that the relative nucleotide levels more nearly reflect actual intracellular conditions. With this system the rate of initiation is highly sensitive to changes in the ADP:ATP and GDP:GTP ratios, but indifferent to the absolute levels of either diphosphate. While the tight coupling of these two ratios by endogenous nucleoside diphosphate kinase activity prevents the independent manipulation of either ratio, the data do eliminate both AMP and GMP per se as inhibitory species. The close agreement of our data calculated in terms of energy charge to previously published results on overall rates of protein synthesis in rat thymocytes (Mendelsohn, S.K., Nordeen, S.K., and Young, D.A. (1977) Biochem. Biophys. Res. Commun. 79, 53-60) continues to suggest a physiologically relevant regulatory influence of subtle changes in nucleotides acting at the level of the initiation reaction. PMID:2999123

  19. LGI1 acts presynaptically to regulate excitatory synaptic transmission during early postnatal development.

    PubMed

    Boillot, Morgane; Lee, Chun-Yao; Allene, Camille; Leguern, Eric; Baulac, Stéphanie; Rouach, Nathalie

    2016-01-01

    The secreted leucine-rich glioma inactivated 1 (LGI1) protein is an important actor for human seizures of both genetic and autoimmune etiology: mutations in LGI1 cause inherited temporal lobe epilepsy, while LGI1 is involved in antibody-mediated encephalitis. Remarkably, Lgi1-deficient (Lgi1(-/-)) mice recapitulate the epileptic disorder and display early-onset spontaneous seizures. To understand how Lgi1-deficiency leads to seizures during postnatal development, we here investigated the early functional and structural defects occurring before seizure onset in Lgi1(-/-) mice. We found an increased excitatory synaptic transmission in hippocampal slices from Lgi1(-/-) mice. No structural alteration in the morphology of pyramidal cell dendrites and synapses was observed at this stage, indicating that Lgi1-deficiency is unlikely to trigger early developmental abnormalities. Consistent with the presynaptic subcellular localization of the protein, Lgi1-deficiency caused presynaptic defects, with no alteration in postsynaptic AMPA receptor activity in Lgi1-/- pyramidal cells before seizure onset. Presynaptic dysfunction led to increased synaptic glutamate levels, which were associated with hyperexcitable neuronal networks. Altogether, these data show that Lgi1 acts presynaptically as a negative modulator of excitatory synaptic transmission during early postnatal development. We therefore here reveal that increased presynaptic glutamate release is a key early event resulting from Lgi1-deficiency, which likely contributes to epileptogenesis. PMID:26878798

  20. LGI1 acts presynaptically to regulate excitatory synaptic transmission during early postnatal development

    PubMed Central

    Boillot, Morgane; Lee, Chun-Yao; Allene, Camille; Leguern, Eric; Baulac, Stéphanie; Rouach, Nathalie

    2016-01-01

    The secreted leucine-rich glioma inactivated 1 (LGI1) protein is an important actor for human seizures of both genetic and autoimmune etiology: mutations in LGI1 cause inherited temporal lobe epilepsy, while LGI1 is involved in antibody-mediated encephalitis. Remarkably, Lgi1-deficient (Lgi1−/−) mice recapitulate the epileptic disorder and display early-onset spontaneous seizures. To understand how Lgi1-deficiency leads to seizures during postnatal development, we here investigated the early functional and structural defects occurring before seizure onset in Lgi1−/− mice. We found an increased excitatory synaptic transmission in hippocampal slices from Lgi1−/− mice. No structural alteration in the morphology of pyramidal cell dendrites and synapses was observed at this stage, indicating that Lgi1-deficiency is unlikely to trigger early developmental abnormalities. Consistent with the presynaptic subcellular localization of the protein, Lgi1-deficiency caused presynaptic defects, with no alteration in postsynaptic AMPA receptor activity in Lgi1−/− pyramidal cells before seizure onset. Presynaptic dysfunction led to increased synaptic glutamate levels, which were associated with hyperexcitable neuronal networks. Altogether, these data show that Lgi1 acts presynaptically as a negative modulator of excitatory synaptic transmission during early postnatal development. We therefore here reveal that increased presynaptic glutamate release is a key early event resulting from Lgi1-deficiency, which likely contributes to epileptogenesis. PMID:26878798

  1. Ubiquitin-Fold Modifier 1 Acts as a Positive Regulator of Breast Cancer

    PubMed Central

    Yoo, Hee Min; Park, Jong Ho; Jeon, Young Joo; Chung, Chin Ha

    2015-01-01

    Estrogen receptor-α (ERα) is a steroid hormone-sensitive transcription factor that plays a critical role in development of breast cancer. The binding of estrogen to ERα triggers the recruitment of transcriptional co-activators as well as chromatin remodeling factors to estrogen-responsive elements (ERE) of ERα target genes. This process is tightly associated with post-translational modifications (PTMs) of ERα and its co-activators for promotion of transcriptional activation, which leads to proliferation of a large subset of breast tumor cells. These PTMs include phosphorylation, acetylation, methylation, and conjugation by ubiquitin and ubiquitin-like proteins. Ubiquitin-fold modifier 1 (UFM1), one of ubiquitin-like proteins, has recently been shown to be ligated to activating signal co-integrator 1 (ASC1), which acts as a transcriptional co-activator of nuclear receptors. Here, we discuss the mechanistic connection between ASC1 modification by UFM1 and ERα transactivation, and highlight how the interplay of these processes is involved in development of breast cancer. We also discuss potential use of UFM1-conjugating system as therapeutic targets against not only breast cancer but also other nuclear receptor-mediated cancers. PMID:25852645

  2. 13 CFR 126.304 - What must a concern submit to SBA?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What must a concern submit to SBA? 126.304 Section 126.304 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION HUBZONE PROGRAM Certification § 126.304 What must a concern submit to SBA? (a) To be certified by SBA as a qualified HUBZone SBC, a concern must submit a...

  3. Adsorption of lysozyme on hyaluronic acid functionalized SBA-15 mesoporous silica: a possible bioadhesive depot system.

    PubMed

    Medda, Luca; Casula, Maria F; Monduzzi, Maura; Salis, Andrea

    2014-11-01

    Silica-based ordered mesoporous materials are very attractive matrices to prepare smart depot systems for several kinds of therapeutic agents. This work focuses on the well-known SBA-15 mesoporous silica and lysozyme, an antimicrobial protein. In order to improve the bioadhesion properties of SBA-15 particles, the effect of hyaluronic acid (HA) functionalization on lysozyme adsorption was investigated. SBA-15 samples having high (H-SBA) and low (L-SBA) levels of functionalization were analyzed during the three steps of the preparations: (1) introduction of the -NH2 groups to obtain the SBA-NH2 samples; (2) functionalization with HA to obtain the SBA-HA matrices; (3) adsorption of lysozyme. All silica matrices were characterized through N2-adsorption/desorption isotherms, small-angle X-ray scattering, transmission electron microscopy, thermogravimetric analysis, and Fourier transform infrared spectroscopy. The whole of the experimental data suggests that a high level of functionalization of the silica surface allows for a negligible lysozyme adsorption mainly due to unfavorable electrostatic interactions (H-SBA-NH2) or steric hindrance (H-SBA-HA). A low degree of functionalization of the silica surface brings about a very good performance toward lysozyme adsorption, being 71% (L-SBA-NH2) and 63% (L-SBA-HA) respectively, compared to that observed for original SBA-15. Finally, two different kinetic models--a "pseudo-second order" and a "intraparticle diffusion"--were compared to fit lysozyme adsorption data, the latter being more reliable than the former. PMID:25295387

  4. Paralogous Antirepressors Acting on the Master Regulator for Biofilm Formation in Bacillus subtilis

    PubMed Central

    Chai, Yunrong; Kolter, Roberto; Losick, Richard

    2009-01-01

    Summary Matrix production during biofilm formation by Bacillus subtilis is governed by a gene control circuit at the heart of which are three dedicated regulatory proteins, the antirepressor SinI, the repressor SinR, and the downstream regulator SlrR. Matrix production is triggered by the synthesis of SinI, which binds to and inactivates SinR, thereby derepressing genes for matrix production as well as the gene for SlrR. Recently, two additional regulators of matrix genes were identified: SlrA, which was reported to be an activator of SlrR, and YwcC, a repressor of SlrA synthesis. We present evidence indicating that SlrA, which is a paralog of SinI, is like SinI, an antirepressor that binds to, and inactivates, SinR. We also show that SlrA does not activate SlrR for expression of matrix genes. Instead, SlrR binds to, and inhibits the activity of, SlrA. Thus, the YwcC-SlrA-SinR-SlrR pathway is a negative feedback loop in which SlrA indirectly stimulates the synthesis of SlrR, and SlrR, in turn, inhibits the activity of SlrA. Finally, we report that under standard laboratory conditions SlrA makes only a small contribution to the expression of genes for matrix production. We propose that in response to an unknown signal recognized by the YwcC repressor, SlrA transiently boosts matrix production. PMID:19788541

  5. Prostanoids regulate angiogenesis acting primarily on IP and EP4 receptors.

    PubMed

    Hoang, Khuyen Gia; Allison, Sarah; Murray, Michael; Petrovic, Nenad

    2015-09-01

    Angiogenesis is regulated by numerous activators and inhibitors, including prostanoids. Although many studies have identified their roles in inflammation, regulatory functions of prostanoids in angiogenesis are poorly understood. Here, we compared the activation of angiogenesis in vitro by two prostanoids with important vascular roles: prostaglandin E2 (PGE2) - thought to be the most important prostanoid activator of angiogenesis - and prostaglandin I2 (prostacyclin or PGI2), whose receptors are predominantly expressed in endothelial cells. Both of these prostanoids activate G-protein coupled receptors: EP1, EP2, EP3 and EP4 by PGE2 and IP by prostacyclin. Human umbilical vein endothelial cells (HUVECs) were used to characterize two pivotal pro-angiogenic processes in vitro: cell migration (using the matrigel droplet assay developed in our laboratory) and "tube formation" (a widely accepted method of assessing formation of blood vessel precursors). The suppression of cell migration and tube formation by the IP-specific antagonist CAY10441 was more extensive (~80%) than by the EP4-specific antagonist L-161,982 (~20%). AH6809, an antagonist of EP1, EP2 and EP3 receptors did not significantly suppress angiogenesis. Expression of the pro-angiogenic receptors KDR and Tie-2 in HUVECs was preferentially suppressed by antagonism of IP and EP4 receptors, respectively. EP4 and IP receptor agonists elicited biphasic actions on angiogenic processes in which there was activation at low concentration, and rapid desensitization at high concentrations - a characteristic common to many G-protein coupled receptors. Together these findings suggest that the prostacyclin-IP pathway plays a major role in the regulation of pro-angiogenic processes in HUVECs. PMID:26188701

  6. DOCK2 and DOCK5 Act Additively in Neutrophils To Regulate Chemotaxis, Superoxide Production, and Extracellular Trap Formation

    PubMed Central

    Watanabe, Mayuki; Terasawa, Masao; Miyano, Kei; Yanagihara, Toyoshi; Uruno, Takehito; Sanematsu, Fumiyuki; Nishikimi, Akihiko; Côté, Jean-François; Sumimoto, Hideki; Fukui, Yoshinori

    2015-01-01

    Neutrophils are highly motile leukocytes that play important roles in the innate immune response to invading pathogens. Neutrophils rapidly migrate to the site of infections and kill pathogens by producing reactive oxygen species (ROS). Neutrophil chemotaxis and ROS production require activation of Rac small GTPase. DOCK2, an atypical guanine nucleotide exchange factor (GEF), is one of the major regulators of Rac in neutrophils. However, because DOCK2 deficiency does not completely abolish fMLF-induced Rac activation, other Rac GEFs may also participate in this process. In this study, we show that DOCK5 acts with DOCK2 in neutrophils to regulate multiple cellular functions. We found that fMLF- and PMA-induced Rac activation were almost completely lost in mouse neutrophils lacking both DOCK2 and DOCK5. Although β2 integrin–mediated adhesion occurred normally even in the absence of DOCK2 and DOCK5, mouse neutrophils lacking DOCK2 and DOCK5 exhibited a severe defect in chemotaxis and ROS production. Similar results were obtained when human neutrophils were treated with CPYPP, a small-molecule inhibitor of these DOCK GEFs. Additionally, we found that DOCK2 and DOCK5 regulate formation of neutrophil extracellular traps (NETs). Because NETs are involved in vascular inflammation and autoimmune responses, DOCK2 and DOCK5 would be a therapeutic target for controlling NET-mediated inflammatory disorders. PMID:25339677

  7. The conserved Misshapen-Warts-Yorkie pathway acts in enteroblasts to regulate intestinal stem cells in Drosophila

    PubMed Central

    Li, Qi; Li, Shuangxi; Mana-Capelli, Sebastian; Roth Flach, Rachel J.; Danai, Laura V.; Amcheslavsky, Alla; Nie, Yingchao; Kaneko, Satoshi; Yao, Xiaohao; Chen, Xiaochu; Cotton, Jennifer L.; Mao, Junhao; McCollum, Dannel; Jiang, Jin; Czech, Michael P.; Xu, Lan; Ip, Y. Tony

    2014-01-01

    SUMMARY Similar to the mammalian intestine, the Drosophila adult midgut has resident stem cells that support growth and regeneration. How the niche regulates intestinal stem cell activity in both mammals and flies is not well understood. Here we show that the conserved germinal center protein kinase Misshapen restricts intestinal stem cell division by repressing the expression of the JAK-STAT pathway ligand Upd3 in differentiating enteroblasts. Misshapen, a distant relative to the prototypic Warts activating kinase Hippo, interacts with and activates Warts to negatively regulate the activity of Yorkie and the expression of Upd3. The mammalian Misshapen homolog MAP4K4 similarly interacts with LATS (Warts homolog) and promotes inhibition of YAP (Yorkie homolog). Together, this work reveals that the Misshapen-Warts-Yorkie pathway acts in enteroblasts to control niche signaling to intestinal stem cells. These findings also provide a model in which to study requirements for MAP4K4-related kinases in MST1/2-independent regulation of LATS and YAP. PMID:25453828

  8. Transferrin synthesis by small cell lung cancer cells acts as an autocrine regulator of cellular proliferation.

    PubMed Central

    Vostrejs, M; Moran, P L; Seligman, P A

    1988-01-01

    Since transferrin is required for cellular proliferation, we investigated transferrin synthesis by a small cell lung cancer line (NCI-H510) that survives in serum-free media without added transferrin. Immunoassays for human transferrin demonstrated that these cells contained immunoreactive human transferrin. Immunofluorescence studies showed that the protein is expressed on the surface of cells, presumably bound to transferrin receptor. Media conditioned by NCI-H510 cells support proliferation of human leukemic cells that would not survive in media lacking transferrin. [35S]Methionine incorporation documented transferrin synthesis by NCI-H510 cells as well as three other small cell lines. Transferrin synthesis by NCI-H510 cells increased more than 10-fold when cells entered active phases of the cell cycle, and this increase was seen before large increases in transferrin-receptor expression. Further experiments examining the effects of agents that affect iron metabolism show that the addition of transferrin-iron or hemin to the media is associated with a more rapid initial rate of proliferation and lower rates of transferrin synthesis than control cells. Gallium salts, which inhibit iron uptake, inhibited proliferation of these cells. If the cells recovered from this effect, transferrin synthesis remained greatly increased compared to control. We conclude that transferrin synthesis by these malignant cells is ultimately related to an iron requirement for cellular proliferation. It appears that this synthesized transferrin acts as part of an important autocrine mechanism permitting proliferation of these cells, and perhaps permitting tumor cell growth in vivo in areas not well vascularized. Images PMID:2839550

  9. National health information privacy: regulations under the Health Insurance Portability and Accountability Act.

    PubMed

    Gostin, L O

    2001-06-20

    Health information privacy is important in US society, but existing federal and state law does not offer adequate protection. The Department of Health and Human Services, under powers granted by the Health Insurance Portability and Accountability Act of 1996, recently issued a final rule providing systematic, nationwide health information privacy protection. The rule is extensive in its scope, applying to health plans, health care clearinghouses, and health care providers (hospitals, clinics, and health departments) who conduct financial transactions electronically ("covered entities"). The rule applies to personally identifiable information in any form, whether communicated electronically, on paper, or orally. The rule does not preempt state law that affords more stringent privacy protection; thus, the health care industry will have to comply with multiple layers of federal and state law. The rule affords patients rights to education about privacy safeguards, access to their medical records, and a process for correction of records. It also requires the patient's permission for disclosures of personal information. While privacy is an important value, it may conflict with public responsibilities to use data for social goods. The rule has special provisions for disclosure of health information for research, public health, law enforcement, and commercial marketing. The privacy debate will continue in Congress and within the president's administration. The primary focus will be on the costs and burdens on health care providers, the ability of health care professionals to use and share full medical information when treating patients, the provision of patient care in a timely and efficient manner, and parents' access to information about the health of their children. PMID:11410101

  10. Selective functionalization of the mesopores of SBA-15

    DOE PAGESBeta

    Webb, Jonathan D.; Seki, Tomohiro; Goldston, Jennifer F.; Pruski, Marek; Crudden, Cathleen M.

    2014-10-23

    In this study, a method has been developed that permits the highly selective functionalization of the interior and exterior surfaces of the ubiquitous mesoporous material, SBA-15. The key step is reloading the as-synthesized material with structure-directing agent, Pluronic® P123, prior to selective functionalization of the external surface with a silylating agent. This new approach represents a significant improvement over literature procedures. Results from physisorption analyses as well as solid-state NMR permit a detailed, quantitative assessment of functionalized SBA-15. This work also provides insight into the stability of the silyl layer during extraction procedures – an issue often neglected in othermore » studies but of significant importance as decomposition of this layer could result in the introduction of new silanols and reduce the effectiveness of any selective grafting procedure.« less

  11. Selective functionalization of the mesopores of SBA-15

    SciTech Connect

    Webb, Jonathan D.; Seki, Tomohiro; Goldston, Jennifer F.; Pruski, Marek; Crudden, Cathleen M.

    2014-10-23

    In this study, a method has been developed that permits the highly selective functionalization of the interior and exterior surfaces of the ubiquitous mesoporous material, SBA-15. The key step is reloading the as-synthesized material with structure-directing agent, Pluronic® P123, prior to selective functionalization of the external surface with a silylating agent. This new approach represents a significant improvement over literature procedures. Results from physisorption analyses as well as solid-state NMR permit a detailed, quantitative assessment of functionalized SBA-15. This work also provides insight into the stability of the silyl layer during extraction procedures – an issue often neglected in other studies but of significant importance as decomposition of this layer could result in the introduction of new silanols and reduce the effectiveness of any selective grafting procedure.

  12. Four Tomato FLOWERING LOCUS T-Like Proteins Act Antagonistically to Regulate Floral Initiation.

    PubMed

    Cao, Kai; Cui, Lirong; Zhou, Xiaoting; Ye, Lin; Zou, Zhirong; Deng, Shulin

    2015-01-01

    The transition from vegetative growth to floral meristems in higher plants is regulated through the integration of internal cues and environmental signals. We were interested to examine the molecular mechanism of flowering in the day-neutral plant tomato (Solanum lycopersicum L.) and the effect of environmental conditions on tomato flowering. Analysis of the tomato genome uncovered 13 PEBP (phosphatidylethanolamine-binding protein) genes, and found six of them were FT-like genes which named as SlSP3D, SlSP6A, SlSP5G, SlSP5G1, SlSP5G2, and SlSP5G3. Six FT-like genes were analyzed to clarify their functional roles in flowering using transgenic and expression analyses. We found that SlSP5G, SlSP5G2, and SlSP5G3 proteins were floral inhibitors whereas only SlSP3D/SFT (SINGLE FLOWER TRUSS) was a floral inducer. SlSP5G was expressed at higher levels in long day (LD) conditions compared to short day (SD) conditions while SlSP5G2 and SlSP5G3 showed the opposite expression patterns. The silencing of SlSP5G by VIGS (Virus induced gene silencing) resulted in tomato plants that flowered early under LD conditions and the silencing of SlSP5G2 and SlSP5G3 led to early flowering under SD conditions. The higher expression levels of SlSP5G under LD conditions were not seen in phyB1 mutants, and the expression levels of SlSP5G2 and SlSP5G3 were increased in phyB1 mutants under both SD and LD conditions compared to wild type plants. These data suggest that SlSP5G, SlSP5G2, and SlSP5G3 are controlled by photoperiod, and the different expression patterns of FT-like genes under different photoperiod may contribute to tomato being a day neutral plant. In addition, PHYB1 mediate the expression of SlSP5G, SlSP5G2, and SlSP5G3 to regulate flowering in tomato. PMID:26793202

  13. Four Tomato FLOWERING LOCUS T-Like Proteins Act Antagonistically to Regulate Floral Initiation

    PubMed Central

    Cao, Kai; Cui, Lirong; Zhou, Xiaoting; Ye, Lin; Zou, Zhirong; Deng, Shulin

    2016-01-01

    The transition from vegetative growth to floral meristems in higher plants is regulated through the integration of internal cues and environmental signals. We were interested to examine the molecular mechanism of flowering in the day-neutral plant tomato (Solanum lycopersicum L.) and the effect of environmental conditions on tomato flowering. Analysis of the tomato genome uncovered 13 PEBP (phosphatidylethanolamine-binding protein) genes, and found six of them were FT-like genes which named as SlSP3D, SlSP6A, SlSP5G, SlSP5G1, SlSP5G2, and SlSP5G3. Six FT-like genes were analyzed to clarify their functional roles in flowering using transgenic and expression analyses. We found that SlSP5G, SlSP5G2, and SlSP5G3 proteins were floral inhibitors whereas only SlSP3D/SFT (SINGLE FLOWER TRUSS) was a floral inducer. SlSP5G was expressed at higher levels in long day (LD) conditions compared to short day (SD) conditions while SlSP5G2 and SlSP5G3 showed the opposite expression patterns. The silencing of SlSP5G by VIGS (Virus induced gene silencing) resulted in tomato plants that flowered early under LD conditions and the silencing of SlSP5G2 and SlSP5G3 led to early flowering under SD conditions. The higher expression levels of SlSP5G under LD conditions were not seen in phyB1 mutants, and the expression levels of SlSP5G2 and SlSP5G3 were increased in phyB1 mutants under both SD and LD conditions compared to wild type plants. These data suggest that SlSP5G, SlSP5G2, and SlSP5G3 are controlled by photoperiod, and the different expression patterns of FT-like genes under different photoperiod may contribute to tomato being a day neutral plant. In addition, PHYB1 mediate the expression of SlSP5G, SlSP5G2, and SlSP5G3 to regulate flowering in tomato. PMID:26793202

  14. Cis-acting elements are required for selenium regulation of glutathione peroxidase-1 mRNA levels.

    PubMed Central

    Weiss, S L; Sunde, R A

    1998-01-01

    Classical glutathione peroxidase (GPX1) mRNA levels can decrease to less than 10% in selenium (Se)-deficient rat liver. The cis-acting nucleic acid sequence requirements for Se regulation of GPX1 mRNA levels were studied by transfecting Chinese hamster ovary (CHO) cells with GPX1 DNA constructs in which specific regions of the GPX1 gene were mutated, deleted, or replaced by comparable regions from unregulated genes such as phospholipid hydroperoxide glutathione peroxidase (GPX4). For each construct, stable transfectants were pooled two weeks after transfection, divided into Se-deficient (2 nM Se) or Se-adequate (200 nM Se) medium, and grown for an additional four days. On day of harvest, Se-deficient GPX1 and GPX4 activities averaged 13 +/- 2% and 15 +/- 2% of Se adequate levels, confirming that cellular Se status was dramatically altered by Se supplementation. RNA was isolated from replicate plates of cells and transfected mRNA levels were specifically determined by RNase protection assay. Analysis of chimeric GPX1/GPX4 constructs showed that the GPX4 3'-UTR can completely replace the GPX1 3'-UTR in Se regulation of GPX1 mRNA. We did not find any GPX1 coding regions that could be replaced by the corresponding GPX4 coding regions without diminishing or eliminating Se regulation of the transfected GPX1 mRNA. Further analysis of the GPX1 coding region demonstrated that the GPX1 Sec codon (UGA) and the GPX1 intron sequences are required for full Se regulation of transfected GPX1 mRNA levels. Mutations that moved the GPX1 Sec codon to three different positions within the GPX1 coding region suggest that the mechanism for Se regulation of GPX1 mRNA requires a Sec codon within exon 1. Lastly, we found that addition of the GPX1 3'-UTR to beta-globin mRNA can convey significant Se regulation to beta-globin mRNA levels when a UGA codon is placed within exon 1. We conclude that Se regulation of GPX1 mRNA requires a functional selenocysteine insertion sequence (SECIS) in the 3

  15. Regulation of DNA replication in irradiated cells by trans-acting factors

    SciTech Connect

    Wang, Y.; Huq, M.S.; Cheng, X.; Iliakis, G.

    1995-05-01

    We compared DNA replication activity in cytoplasmic extracts prepared from irradiated and nonirradiated HeLa cells using a simian virus 40 (SV40)-based in vitro replication assay. The assay measures semi-conservative DNA replication in a plasmid carrying the SV40 origin of replication and requires SV40 T antigen as the sole noncellular protein. The plasmid DNA used in the replication reaction is never exposed to radiation. We find that replication of plasmid DNA is significantly reduced when cytoplasmic extracts from irradiated cells are used. Since plasmid replication proceeds to completion in extracts from irradiated cells, the observed reduction in the overall replication activity is probably due to a reduction in the efficiency of initiation events. The degree of inhibition of DNA replication after exposure to 10, 30 and 50 Gy X rays as measured in vitro using this assay is similar to that measured in intact cells immediately before processing for extract preparation. These observations are compatible with the induction or activation by ionizing radiation of a factor(s) that inhibits in trans DNA replication. The results contribute to our understanding of the mechanism(s) developed by the cells to regulate DNA replication when exposed to clastogenic agents. Such processes may be of significance in the restoration of DNA integrity, and may define yet another checkpoint operating during S at the level of clusters of replicons. 26 refs., 4 figs.

  16. GABAA receptor-acting neurosteroids: A role in the development and regulation of the stress response

    PubMed Central

    Gunn, Benjamin G.; Cunningham, Linda; Mitchell, Scott G.; Swinny, Jerome D.; Lambert, Jeremy J.; Belelli, Delia

    2015-01-01

    Regulation of hypothalamic–pituitary–adrenocortical (HPA) axis activity by stress is a fundamental survival mechanism and HPA-dysfunction is implicated in psychiatric disorders. Adverse early life experiences, e.g. poor maternal care, negatively influence brain development and programs an abnormal stress response by encoding long-lasting molecular changes, which may extend to the next generation. How HPA-dysfunction leads to the development of affective disorders is complex, but may involve GABAA receptors (GABAARs), as they curtail stress-induced HPA axis activation. Of particular interest are endogenous neurosteroids that potently modulate the function of GABAARs and exhibit stress-protective properties. Importantly, neurosteroid levels rise rapidly during acute stress, are perturbed in chronic stress and are implicated in the behavioural changes associated with early-life adversity. We will appraise how GABAAR-active neurosteroids may impact on HPA axis development and the orchestration of the stress-evoked response. The significance of these actions will be discussed in the context of stress-associated mood disorders. PMID:24929099

  17. Vinculin acts as a sensor in lipid regulation of adhesion-site turnover.

    PubMed

    Chandrasekar, Indra; Stradal, Theresia E B; Holt, Mark R; Entschladen, Frank; Jockusch, Brigitte M; Ziegler, Wolfgang H

    2005-04-01

    The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of this interaction are poorly understood. To study the significance of phospholipid binding to vinculin for adhesion-site turnover and cell motility, we constructed a mutant, vinculin-LD, deficient in acidic phospholipid binding yet with functional actin-binding sites. When expressed in cells, vinculin-LD was readily recruited to adhesion sites, as judged by fluorescence recovery after photobleaching (FRAP) analysis, but cell spreading and migration were strongly impaired, and PIP(2)-dependent disassembly of adhesions was suppressed. Thus, PIP(2) binding is not essential for vinculin activation and recruitment, as previously suggested. Instead, we propose that PIP(2) levels can regulate the uncoupling of adhesion sites from the actin cytoskeleton, with vinculin functioning as a sensor. PMID:15769850

  18. FoxG1 and TLE2 act cooperatively to regulate ventral telencephalon formation

    PubMed Central

    Roth, Martin; Bonev, Boyan; Lindsay, Jennefer; Lea, Robert; Panagiotaki, Niki; Houart, Corinne; Papalopulu, Nancy

    2010-01-01

    FoxG1 is a conserved transcriptional repressor that plays a key role in the specification, proliferation and differentiation of the telencephalon, and is expressed from the earliest stages of telencephalic development through to the adult. How the interaction with co-factors might influence the multiplicity and diversity of FoxG1 function is not known. Here, we show that interaction of FoxG1 with TLE2, a Xenopus tropicalis co-repressor of the Groucho/TLE family, is crucial for regulating the early activity of FoxG1. We show that TLE2 is co-expressed with FoxG1 in the ventral telencephalon from the early neural plate stage and functionally cooperates with FoxG1 in an ectopic neurogenesis assay. FoxG1 has two potential TLE binding sites: an N-terminal eh1 motif and a C-terminal YWPMSPF motif. Although direct binding seems to be mediated by the N-terminal motif, both motifs appear important for functional synergism. In the neurogenesis assay, mutation of either motif abolishes functional cooperation of TLE2 with FoxG1, whereas in the forebrain deletion of both motifs renders FoxG1 unable to induce the ventral telencephalic marker Nkx2.1. Knocking down either FoxG1 or TLE2 disrupts the development of the ventral telencephalon, supporting the idea that endogenous TLE2 and FoxG1 work together to specify the ventral telencephalon. PMID:20356955

  19. IL-4 acts as a homeostatic regulator of IL-2-induced TNF and IFN-gamma.

    PubMed Central

    Bello-Fernandez, C; Oblakowski, P; Meager, A; Duncombe, A S; Rill, D M; Hoffbrand, A V; Brenner, M K

    1991-01-01

    Interleukin-4 (IL-4) is a cytokine secreted by interleukin-2 (IL-2)-activated lymphocytes. IL-2-stimulated lymphocytes also secrete two cytokines, tumour necrosis factor (TNF) and gamma-interferon (IFN-gamma), which contribute to effector function and which may themselves recruit fresh, cytokine-secreting effector cells. We have now investigated whether the IL-4 induced is able to homeostatically regulate secretion of the TNF and IFN-gamma. Peripheral blood mononuclear cells or lymphocytes from normal donors and from patients with neoplastic disease were cultured in the presence of IL-2 alone, IL-4 alone or with both cytokines. IL-2 induced high levels of TNF and IFN-gamma secretion in both groups. The addition of recombinant IL-4 to these IL-2-stimulated cultures lead to significant inhibition of IFN-gamma and TNF production. IFN-gamma secretion was reduced by 50-99% in normal donors and by between 11% and 99% in patients (P less than 0.001). TNF levels induced by IL-2 were similarly reduced by IL-4 both in normal donors (P less than 0.003) and in patients (P less than 0.01). These inhibitory effects were produced by IL-4 at doses of IL-2 attainable in vivo. Inhibition appears to represent a homeostatic regulatory mechanism which may limit recruitment of fresh activated killer (AK) cells. When endogenous IL-4 activity in IL-2-activated lymphocytes was blocked by anti-IL-4 antibody, significantly higher levels of IFN-gamma and TNF were secreted (P less than 0.05). Since both TNF and IFN-gamma may contribute to the anti-neoplastic action of IL-2, manipulating the level of IL-4 activity in vivo could augment the benefits of IL-2 immunotherapy. PMID:1901829

  20. The pif1 helicase, a negative regulator of telomerase, acts preferentially at long telomeres.

    PubMed

    Phillips, Jane A; Chan, Angela; Paeschke, Katrin; Zakian, Virginia A

    2015-04-01

    Telomerase, the enzyme that maintains telomeres, preferentially lengthens short telomeres. The S. cerevisiae Pif1 DNA helicase inhibits both telomerase-mediated telomere lengthening and de novo telomere addition at double strand breaks (DSB). Here, we report that the association of the telomerase subunits Est2 and Est1 at a DSB was increased in the absence of Pif1, as it is at telomeres, suggesting that Pif1 suppresses de novo telomere addition by removing telomerase from the break. To determine how the absence of Pif1 results in telomere lengthening, we used the single telomere extension assay (STEX), which monitors lengthening of individual telomeres in a single cell cycle. In the absence of Pif1, telomerase added significantly more telomeric DNA, an average of 72 nucleotides per telomere compared to the 45 nucleotides in wild type cells, and the fraction of telomeres lengthened increased almost four-fold. Using an inducible short telomere assay, Est2 and Est1 no longer bound preferentially to a short telomere in pif1 mutant cells while binding of Yku80, a telomere structural protein, was unaffected by the status of the PIF1 locus. Two experiments demonstrate that Pif1 binding is affected by telomere length: Pif1 (but not Yku80) -associated telomeres were 70 bps longer than bulk telomeres, and in the inducible short telomere assay, Pif1 bound better to wild type length telomeres than to short telomeres. Thus, preferential lengthening of short yeast telomeres is achieved in part by targeting the negative regulator Pif1 to long telomeres. PMID:25906395

  1. The Exosome and Trans-Acting Small Interfering RNAs Regulate Cuticular Wax Biosynthesis during Arabidopsis Inflorescence Stem Development1[OPEN

    PubMed Central

    Lam, Patricia; Zhao, Lifang; Eveleigh, Nathan; Yu, Yu; Chen, Xuemei

    2015-01-01

    The primary aerial surfaces of land plants are covered with a cuticle, a protective layer composed of the cutin polyester matrix and cuticular waxes. Previously, we discovered a unique mechanism of regulating cuticular wax biosynthesis during Arabidopsis (Arabidopsis thaliana) stem elongation that involves ECERIFERUM7 (CER7), a core subunit of the exosome. Because loss-of-function mutations in CER7 result in reduced expression of the wax biosynthetic gene CER3, we proposed that CER7 is involved in degrading a messenger RNA encoding a CER3 repressor. To identify this putative repressor, we performed a cer7 suppressor screen that resulted in the isolation of the posttranscriptional gene-silencing components RNA-DEPENDENT RNA POLYMERASE1 and SUPPRESSOR OF GENE SILENCING3, indicating that small RNAs regulate CER3 expression. To establish the identity of the effector RNA species and determine whether these RNAs control CER3 transcript levels directly, we cloned additional genes identified in our suppressor screen and performed next-generation sequencing of small RNA populations that differentially accumulate in the cer7 mutant in comparison with the wild type. Our results demonstrate that the trans-acting small interfering RNA class of small RNAs are the effector molecules involved in direct silencing of CER3 and that the expression of five additional genes (EARLY RESPONSE TO DEHYDRATION14, AUXIN RESISTANT1, a translation initiation factor SUI1 family protein, and two genes of unknown function) is controlled by both CER7 and trans-acting small interfering RNAs. PMID:25502190

  2. Diversification of a Transcription Factor Family Led to the Evolution of Antagonistically Acting Genetic Regulators of Root Hair Growth.

    PubMed

    Breuninger, Holger; Thamm, Anna; Streubel, Susanna; Sakayama, Hidetoshi; Nishiyama, Tomoaki; Dolan, Liam

    2016-06-20

    Streptophytes colonized the land some time before 470 million years ago [1-3]. The colonization coincided with an increase in morphological and cellular diversity [4-7]. This increase in diversity is correlated with a proliferation in transcription factors encoded in genomes [8-10]. This suggests that gene duplication and subsequent diversification of function was instrumental in the generation of land plant diversity. Here, we investigate the diversification of the streptophyte-specific Lotus japonicus ROOTHAIRLESS LIKE (LRL) transcription factor (TF) [11, 12] subfamily of basic loop helix (bHLH) proteins by comparing gene function in early divergent and derived land plant species. We report that the single Marchantia polymorpha LRL gene acts as a general growth regulator required for rhizoid development, a function that has been partially conserved throughout multicellular streptophytes. In contrast, the five relatively derived Arabidopsis thaliana LRL genes comprise two antagonistically acting groups of differentially expressed genes. The diversification of LRL genes accompanied the evolution of an antagonistic regulatory element controlling root hair development. PMID:27265398

  3. 13 CFR 120.1830 - What are the terms and conditions of an SBA loan to an SISMBD?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... will be $10,000,000 and the maximum size of a SISMBD Loan at the time of loan approval will be equal to... collateral assignment, of the SBA Form 1086 (Secondary Market Participation Agreement) or SBA Form 1088...

  4. 13 CFR 107.1640 - SBA access to records of the CRA, Brokers, Dealers and Pool or Trust assemblers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... (Leverage) Funding Leverage by Use of Sba-Guaranteed Trust Certificates (âtcsâ) § 107.1640 SBA access to... and copying purposes. Such access shall be at such party's primary place of business during...

  5. 13 CFR 102.36 - Privacy Act standards of conduct.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... accounting in the specified form of all disclosures of records by the SBA to persons, organizations, or... employees of the provisions of the Privacy Act, including its civil liability and criminal penalty.... 102.36 Section 102.36 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION RECORD...

  6. The long noncoding RNA Six3OS acts in trans to regulate retinal development by modulating Six3 activity

    PubMed Central

    2011-01-01

    Background Thousands of different long non-coding RNAs are expressed during embryonic development, but the function of these molecules remains largely unexplored. Results Here we characterize the expression and function of Six3OS, a long non-coding RNA that is transcribed from the distal promoter region of the gene encoding the homeodomain transcription factor Six3. Overexpression and knockdown analysis of Six3OS reveals that it plays an essential role in regulating retinal cell specification. We further observe that Six3OS regulates Six3 activity in developing retina, but does not do so by modulating Six3 expression. Finally, we show that Six3OS binds directly to Ezh2 and Eya family members, indicating that Six3OS can act as a molecular scaffold to recruit histone modification enzymes to Six3 target genes. Conclusions Our findings demonstrate a novel mechanism by which promoter-associated long non-coding RNAs can modulate the activity of their associated protein coding genes, and highlight the importance of this diverse class of molecules in the control of neural development. PMID:21936910

  7. c-Myb acts in parallel and cooperatively with Cebp1 to regulate neutrophil maturation in zebrafish.

    PubMed

    Jin, Hao; Huang, Zhibin; Chi, Yali; Wu, Mei; Zhou, Riyang; Zhao, Lingfeng; Xu, Jin; Zhen, Fenghua; Lan, Yahui; Li, Li; Zhang, Wenqing; Wen, Zilong; Zhang, Yiyue

    2016-07-21

    Neutrophils are the key effectors for generating innate immunity in response to pathogenic infection and tissue injury in vertebrates. Dysregulation of neutrophil development and function is known to associate with various human disorders. Yet, the genetic network that orchestrates lineage commitment, differentiation, and maturation of neutrophils remains incompletely defined. Here, we present an in vivo study to delineate the genetic program underlying neutrophil development during zebrafish embryonic myelopoiesis. We show that loss of c-Myb function has no effect on macrophages but severely impairs neutrophil terminal differentiation, resulting in the accumulation of neutrophils with unsegmented nuclei and scant granule. This neutrophilic defect, which resembles the neutrophil-specific granule deficiency (SGD) caused by the mutations in CCAAT/enhancer-binding protein ε (C/EBPε) in humans, is attributed, at least in part, to the downregulation of the granule protein transcription. Likewise, genetic inactivation of Cebp1, the zebrafish functional homolog of mammalian C/EBPε, also leads to a similar SGD-like phenotype in zebrafish. Genetic epistasis and biochemical analysis further reveals that c-Myb and Cebp1 act in parallel and cooperatively to control neutrophil differentiation by directly regulating granule protein gene transcription. Our study indicates that c-MYB is an intrinsic master regulator for neutrophil terminal differentiation and a potential target in SGD patients. PMID:27268086

  8. Hsa-miR-495 acts as a tumor suppressor gene in glioma via the negative regulation of MYB.

    PubMed

    Zhang, Benping; Yuan, Fei; Liu, Jie; Li, Yang; Zhou, Fucheng; Liu, Xuanxi; Hao, Zhen; Li, Qingsong; Zheng, Yongri; Wang, Weizhi

    2016-07-01

    MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression at the post-transcriptional level. Previous studies have reported that there are causative links between the abnormal regulation of miRNAs and cancer development. Hsa‑miR‑495 has previously been demonstrated to be downregulated, and to function as a tumor suppressor, in numerous types of human cancer. However, the function and molecular mechanism of hsa‑miR‑495 in glioma remains unclear. In the current study, the expression and effects of hsa‑miR‑495 on glioma were evaluated. It was identified that the expression levels of hsa-miR-495 were downregulated in glioma tissues and cell lines. Furthermore, restoration of hsa-miR-495 inhibited glioma cell proliferation and invasion in vitro. Notably, a luciferase reporter assay revealed that hsa‑miR‑495 was able to directly target v‑myb avian myeloblastosis viral oncogene homolog (MYB) in glioma cells. In addition, an RNA interference assay indicated that MYB knockdown inhibited glioma cell proliferation and invasion in vitro. In conclusion, the results of the present study suggested that hsa‑miR‑495 may act as a tumor suppressor gene in glioma by directly inhibiting MYB expression, which may provide a novel therapeutic strategy for the treatment of glioma. PMID:27220777

  9. CK2 acts as a potent negative regulator of receptor-mediated insulin release in vitro and in vivo.

    PubMed

    Rossi, Mario; Ruiz de Azua, Inigo; Barella, Luiz F; Sakamoto, Wataru; Zhu, Lu; Cui, Yinghong; Lu, Huiyan; Rebholz, Heike; Matschinsky, Franz M; Doliba, Nicolai M; Butcher, Adrian J; Tobin, Andrew B; Wess, Jürgen

    2015-12-01

    G protein-coupled receptors (GPCRs) regulate virtually all physiological functions including the release of insulin from pancreatic β-cells. β-Cell M3 muscarinic receptors (M3Rs) are known to play an essential role in facilitating insulin release and maintaining proper whole-body glucose homeostasis. As is the case with other GPCRs, M3R activity is regulated by phosphorylation by various kinases, including GPCR kinases and casein kinase 2 (CK2). At present, it remains unknown which of these various kinases are physiologically relevant for the regulation of β-cell activity. In the present study, we demonstrate that inhibition of CK2 in pancreatic β-cells, knockdown of CK2α expression, or genetic deletion of CK2α in β-cells of mutant mice selectively augmented M3R-stimulated insulin release in vitro and in vivo. In vitro studies showed that this effect was associated with an M3R-mediated increase in intracellular calcium levels. Treatment of mouse pancreatic islets with CX4945, a highly selective CK2 inhibitor, greatly reduced agonist-induced phosphorylation of β-cell M3Rs, indicative of CK2-mediated M3R phosphorylation. We also showed that inhibition of CK2 greatly enhanced M3R-stimulated insulin secretion in human islets. Finally, CX4945 treatment protected mice against diet-induced hyperglycemia and glucose intolerance in an M3R-dependent fashion. Our data demonstrate, for the first time to our knowledge, the physiological relevance of CK2 phosphorylation of a GPCR and suggest the novel concept that kinases acting on β-cell GPCRs may represent novel therapeutic targets. PMID:26598688

  10. p53 Acts as a Co-Repressor to Regulate Keratin 14 Expression during Epidermal Cell Differentiation

    PubMed Central

    Chao, Chung-Faye; Lu, Mei-Hua; Lin, Hwang-Chi; Chiou, Shih-Hwa; Tao, Pao-Luh; Chen, Jang-Yi

    2012-01-01

    During epidermal cell differentiation, keratin 14 (K14) expression is down-regulated, p53 expression varies, and the expression of the p53 target genes, p21 and 14-3-3σ, increases. These trends suggest that the relative transcriptional activity of p53 is increased during epidermal cell differentiation. To determine the relationship between K14 and p53, we constructed K14 promoters of various sizes and found that wild-type p53 could repress the promoter activity of all of the K14 promoter constructs in H1299 cells. K14-p160 contains an SP1 binding site mutation that prevents p53 from repressing K14 expression. Using a DNA affinity precipitation assay, we confirmed that p53 forms a complex with SP1 at the SP1 binding site between nucleotides -48 and -43 on the K14 promoter. Thus, our data indicate that p53 acts as a co-repressor to down-regulate K14 expression by binding to SP1. Next, we used a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced epidermal cell differentiation model to examine the inhibition of K14 expression caused by increased p53 activity. Human ovarian teratocarcinoma C9 cells were treated with TPA to induce differentiation. Over-expression of the dominant negative p53 mutant ΔTAp53, which inhibits p53 activity, prevented the TPA-induced K14 down-regulation in C9 cells. Furthermore, treatment of normal primary human foreskin keratinocytes (PHFK) with the p53 inhibitor pifithrin-α (PFT-α) showed that the inhibition of p53 activity relieves K14 repression during epidermal cell differentiation. Finally, we found that TPA induces the phosphorylation of p53 at residue 378, which enhances the affinity of p53 to bind to Sp1 and repress K14 expression. PMID:22911849

  11. 13 CFR 107.1810 - Events of default and SBA's remedies for Licensee's noncompliance with terms of Debentures.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... you at least 15 days to cure the default(s); and (ii) You fail to cure the default(s) to SBA's... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Events of default and SBA's... Noncompliance With Terms of Leverage § 107.1810 Events of default and SBA's remedies for...

  12. 13 CFR 108.1810 - Events of default and SBA's remedies for NMVC Company's noncompliance with terms of Debentures.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Events of default and SBA's... Company's Noncompliance With Terms of Leverage § 108.1810 Events of default and SBA's remedies for NMVC...) Voluntary assignment. You make a voluntary assignment for the benefit of creditors without SBA's...

  13. 13 CFR 120.433 - What are SBA's other requirements for sales and sales of participating interests?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Lender has satisfactory SBA performance, as determined by SBA in its discretion. The Lender's Risk Rating... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are SBA's other requirements for sales and sales of participating interests? 120.433 Section 120.433 Business Credit and...

  14. 28 CFR Appendix D to Subpart G of... - OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) D..., App. D Appendix D to Subpart G of Part 42—OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) Editorial Note: For...

  15. 28 CFR Appendix D to Subpart G of... - OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) D..., App. D Appendix D to Subpart G of Part 42—OJARS' Regulations Under the Omnibus Crime Control and Safe Streets Act, as Amended, Which Apply to This Subpart (28 CFR 42.205 and 42.206) Editorial Note: For...

  16. 13 CFR 126.304 - What must a concern submit to SBA?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Titles and Records Offices and their jurisdiction in 25 CFR 150.4 and 150.5. (c) Concerns applying for... set forth in § 126.200. After submitting the application, applicants must notify SBA of any material... notify SBA of a material change affecting its eligibility pursuant to § 126.501, it must include with...

  17. 13 CFR 126.307 - Where will SBA maintain the List of qualified HUBZone SBCs?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Where will SBA maintain the List of qualified HUBZone SBCs? 126.307 Section 126.307 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION HUBZONE PROGRAM Certification § 126.307 Where will SBA maintain the List of qualified HUBZone SBCs? Qualified HUBZone SBCs are...

  18. In situ catalytic pyrolysis of miscanthus over modified SBA-15 catalysts using Py-GC/MS.

    PubMed

    Lee, Eun Hwa; Jeon, Mi-Jin; Jeon, Jong-Ki; Suh, Dong Jin; Park, Sung Hoon; Seo, Bora; Joo, Sang Hoon; Park, Young-Kwon

    2014-03-01

    Various SBA-15-based catalysts, Si-SBA-15, Pt/Si-SBA-15, Al-SBA-15, and Pt/Al-SBA-15, were applied to the catalytic pyrolysis of miscanthus. Pt nanoparticles with three different sizes, 1.7 nm, 2.9 nm, and 7.1 nm, were used to synthesize Pt/Si-SBA-15 and Pt/Al-SBA-15. Pyrolysis-gas chromatography/mass spectrometry was used for the pyrolysis experiments. The catalysts were characterized by X-ray diffraction patterns, transmittance electron microscopy, N2 adsorption-desorption, and Brunaure-Emmett-Teller surface area. The product species distribution of pyrolysis of miscanthus was significantly affected by the acid property of the catalyst and the presence of Pt. In particular, Pt/Al-SBA-15, which has both acid sites and Pt, changed the product species distribution to the largest extent; the main products were phenolics and furans. The effect of Pt particle size on the species distribution of pyrolysis product was negligible. PMID:24745230

  19. 13 CFR 107.1600 - SBA authority to issue and guarantee Trust Certificates.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA authority to issue and guarantee Trust Certificates. 107.1600 Section 107.1600 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES SBA Financial Assistance for Licensees (Leverage)...

  20. 13 CFR 120.713 - Does SBA provide technical assistance to Intermediaries?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Does SBA provide technical assistance to Intermediaries? 120.713 Section 120.713 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Microloan Program § 120.713 Does SBA provide technical assistance to...

  1. 13 CFR 101.106 - Does Federal law apply to SBA programs and activities?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Does Federal law apply to SBA programs and activities? 101.106 Section 101.106 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.106 Does Federal law apply to SBA programs and activities? (a)...

  2. 13 CFR 101.104 - What are the functions of SBA's field offices?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are the functions of SBA's field offices? 101.104 Section 101.104 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Overview § 101.104 What are the functions of SBA's field offices? (a) Regional offices....

  3. 13 CFR 120.715 - Does SBA guarantee any loans an Intermediary obtains from another source?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Does SBA guarantee any loans an Intermediary obtains from another source? 120.715 Section 120.715 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Microloan Program § 120.715 Does SBA guarantee any loans an Intermediary...

  4. 13 CFR 107.1910 - Non-waiver of SBA's rights or terms of Leverage security.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Non-waiver of SBA's rights or terms of Leverage security. 107.1910 Section 107.1910 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Miscellaneous § 107.1910 Non-waiver of SBA's rights or...

  5. 75 FR 13329 - Implications of Financial Accounting System (FAS) 166 on SBA Guaranteed Loan Programs

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... ADMINISTRATION Implications of Financial Accounting System (FAS) 166 on SBA Guaranteed Loan Programs AGENCY... Administration (SBA) is soliciting information and views from the public on: (1) The effect that the accounting changes mandated by the Financial Accounting Standards Board (FASB) in Financial Accounting Standard...

  6. 13 CFR 120.470 - What are SBA's additional requirements for SBLCs?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are SBA's additional requirements for SBLCs? 120.470 Section 120.470 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Lenders Small Business Lending Companies (sblc) § 120.470 What are SBA's...

  7. 13 CFR 125.27 - How will SBA process an SDVO protest?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How will SBA process an SDVO protest? 125.27 Section 125.27 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION GOVERNMENT CONTRACTING PROGRAMS Protests Concerning SDVO SBCs § 125.27 How will SBA process an SDVO protest? (a) Notice of receipt of protest. Upon receipt of...

  8. 13 CFR 120.223 - Subsidy recoupment fee payable to SBA by Borrower.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Subsidy recoupment fee payable to SBA by Borrower. 120.223 Section 120.223 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Policies Specific to 7(a) Loans Fees for Guaranteed Loans § 120.223 Subsidy recoupment fee payable to SBA by Borrower. (a)...

  9. 13 CFR 127.404 - What happens if SBA is unable to verify a concern's eligibility?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What happens if SBA is unable to verify a concern's eligibility? 127.404 Section 127.404 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION WOMEN-OWNED SMALL BUSINESS FEDERAL CONTRACT ASSISTANCE PROCEDURES Eligibility Examinations § 127.404 What happens if SBA is unable...

  10. Innovative Approaches to Meeting Entrepreneurial Informational Needs: SBA Enters the XXI Century.

    ERIC Educational Resources Information Center

    Solomon, George T.; Fernald, Lloyd W., Jr.

    1993-01-01

    This article points out the critical importance of up-to-date information for small business entrepreneurs and describes two innovative initiatives of the U.S. Small Business Administration (SBA): the national electronic bulletin board system called "SBA On-Line" and Business Information Centers in field locations. (JDD)

  11. 13 CFR 108.1600 - SBA authority to issue and guarantee Trust Certificates.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false SBA authority to issue and guarantee Trust Certificates. 108.1600 Section 108.1600 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW MARKETS VENTURE CAPITAL (âNMVCâ) PROGRAM SBA Financial Assistance for NMVC Companies (Leverage) Funding Leverage by Use of...

  12. 13 CFR 127.604 - How will SBA process an EDWOSB or WOSB status protest?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... a notice of dismissal, citing the reason(s) for the dismissal. Notwithstanding SBA's dismissal of... determination. SBA's determination is effective immediately and is final unless overturned by OHA on appeal... cured the reason(s) for its ineligibility may request an examination under the procedures set forth...

  13. 32 CFR 644.508 - Agreement with Small Business Administration (SBA) on sale of timber.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Agreement with Small Business Administration (SBA..., Crops, and Embedded Gravel, Sand and Stone § 644.508 Agreement with Small Business Administration (SBA... administration of the Army's timber disposal program....

  14. 13 CFR 101.401 - What programs and activities of SBA are subject to this subpart?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What programs and activities of SBA are subject to this subpart? 101.401 Section 101.401 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION ADMINISTRATION Intergovernmental Partnership § 101.401 What programs and activities of SBA...

  15. 13 CFR 107.470 - SBA approval of merger, consolidation, or reorganization of Licensee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... BUSINESS ADMINISTRATION SMALL BUSINESS INVESTMENT COMPANIES Changes in Ownership, Control, or Structure of Licensee; Transfer of License Change in Structure of Licensee § 107.470 SBA approval of merger..., change form of organization (corporation or partnership) or reorganize without SBA's prior...

  16. 13 CFR 126.306 - How will SBA process the certification?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How will SBA process the certification? 126.306 Section 126.306 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION HUBZONE PROGRAM Certification § 126.306 How will SBA process the certification? (a) The D/HUB or designee...

  17. 13 CFR 126.306 - How will SBA process the certification?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false How will SBA process the certification? 126.306 Section 126.306 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION HUBZONE PROGRAM Certification § 126.306 How will SBA process the certification? (a) The D/HUB or designee...

  18. 13 CFR 126.202 - Who does SBA consider to control a HUBZone SBC?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Who does SBA consider to control a HUBZone SBC? 126.202 Section 126.202 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION HUBZONE PROGRAM Requirements to be a Qualified HUBZone SBC § 126.202 Who does SBA consider to control a HUBZone SBC? Control means both the...

  19. Silylated Co/SBA-15 catalysts for Fischer-Tropsch synthesis

    SciTech Connect

    Jia Lihong; Jia Litao; Li Debao; Hou Bo; Wang Jungang; Sun Yuhan

    2011-03-15

    A series of silylated Co/SBA-15 catalysts were prepared via the reaction of surface Si-OH of SBA-15 with hexamethyldisilazane (HMDS) under anhydrous, vapor-phase conditions, and then characterized by FT-IR, N{sub 2} physisorption, TG, XRD, and TPR-MS. The results showed that organic modification led to a silylated SBA-15 surface composed of stable hydrophobic Si-(CH{sub 3}){sub 3} species even after calcinations and H{sub 2} reduction at 673 K. Furthermore, the hydrophobic surface strongly influenced both metal dispersion and reducibility. Compared with non-silylated Co/SBA, Co/S-SBA (impregnation after silylation) showed a high activity, due to the better cobalt reducibility on the hydrophobic support. However, S-Co/SBA (silylation after impregnation) had the lowest FT activity among all the catalysts, due to the lower cobalt reducibility along with the steric hindrance of grafted -Si(CH{sub 3}){sub 3} for the re-adsorption of {alpha}-olefins. -- Graphical abstract: The silylation of an SBA-15 before cobalt impregnation enhanced the reducibility of cobalt oxides on an SBA-15-supported cobalt catalyst and consequently increased the catalytic activity for Fischer-Tropsch synthesis. Display Omitted

  20. 13 CFR 127.303 - How will SBA select and identify approved certifiers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How will SBA select and identify approved certifiers? 127.303 Section 127.303 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION WOMEN-OWNED SMALL BUSINESS FEDERAL CONTRACT ASSISTANCE PROCEDURES Certification of EDWOSB or WOSB Status § 127.303 How will SBA select...