Science.gov

Sample records for action investigation activities

  1. Investigation of the peak action wavelength of light-activated gene transduction.

    PubMed

    Myakishev-Rempel, M; Kuper, J; Mintz, B; Hutchinson, S; Voris, J; Zavislan, K; Offley, S; Nardia, F B; Yaseen, Z; Yen, T; Zavislan, J M; Maloney, M D; Schwarz, E M

    2011-11-01

    Light-activated gene transduction (LAGT) is an approach to localize gene therapy via preactivation of cells with UV light, which facilitates transduction by recombinant adeno-associated virus vectors. Previous studies demonstrated that UVC induces LAGT secondary to pyrimidine dimer formation, whereas UVA induces LAGT secondary to reactive-oxygen species (ROS) generation. However, the empirical UVB boundary of these UV effects is unknown. Thus, we aimed to define the action spectra for UV-induced LAGT independent of DNA damage and determine an optimal wavelength to maximize safety and efficacy. UV at 288, 311 and 320 nm produced significant dose-dependent LAGT effects, of which the maximum (800-fold) was observed with 4 kJ m⁻² at 311 nm. Consistent with its robust cytotoxicity, 288 nm produced significantly high levels of DNA damage at all doses tested, whereas 311, 320 and 330 nm did not generate pyrimidine dimers and produced low levels of DNA damage detected by comet assay. Although 288 nm failed to induce ROS, the other wavelengths were effective, with the maximum (10-fold) effect observed with 30 kJ m⁻² at 311 nm. An in vivo pilot study assessing 311 nm-induced LAGT of rabbit articular chondrocytes demonstrated a significant 6.6-fold (P<0.05) increase in transduction with insignificant cytotoxicity. In conclusion, 311 nm was found to be the optimal wavelength for LAGT on the basis of its superior efficacy at the peak dose and its broad safety range that is remarkably wider than the other UV wavelengths tested.

  2. Investigation of the peak action wavelength of light-activated gene transduction (LAGT)

    PubMed Central

    Myakishev-Rempel, Max; Kuper, Jerry; Mintz, Benjamin; Hutchinson, Sara; Voris, Jay; Zavislan, Katrina; Offley, Sarah; Nardia, Frances Barg; Yaseen, Zaneb; Yen, Tony; Zavislan, James; Maloney, Michael D.; Schwarz, Edward M.

    2011-01-01

    Light-activated gene transduction (LAGT) is an approach to localize gene therapy via preactivation of cells with UV light, which facilitates transduction by recombinant adeno-associated virus vectors. Prior studies demonstrated that UVC induces LAGT secondary to pyrimidine dimer formation, while UVA induces LAGT secondary to reactive oxygen species (ROS) generation. However, the empirical UVB boundary of these UV effects is unknown. Thus, we aimed to define the action spectra for UV-induced LAGT independent of DNA damage, and determine an optimal wavelength to maximize safety and efficacy. Results: UV at 288, 311 and 320nm produced significant dose-dependent LAGT effects, of which the maximum (800-fold) was observed with 4kJ/m2 at 311nm. Consistent with its robust cytotoxicity, 288nm produced significantly high levels of DNA damage at all doses tested, while 311, 320 and 330nm did not generate pyrimidine dimers and produced low levels of DNA damage detected by comet assay. While 288nm failed to induce ROS, the other wavelengths were effective, with the maximum (10-fold) effect observed with 30 kJ/m2 at 311nm. An in vivo pilot study assessing 311nm-induced LAGT of rabbit articular chondrocytes demonstrated a significant 6.6-fold (p<0.05) increase in transduction with insignificant cytotoxicity. Conclusion: 311nm was found to be the optimal wavelength for LAGT based on its superior efficacy at the peak dose, and its broad safety range that is remarkably wider than the other UV wavelengths tested. PMID:21490685

  3. Semantic Activation in Action Planning

    ERIC Educational Resources Information Center

    Lindemann, Oliver; Stenneken, Prisca; van Schie, Hein T.; Bekkering, Harold

    2006-01-01

    Four experiments investigated activation of semantic information in action preparation. Participants either prepared to grasp and use an object (e.g., to drink from a cup) or to lift a finger in association with the object's position following a go/no-go lexical-decision task. Word stimuli were consistent to the action goals of the object use…

  4. Differences in anti-malarial activity of 4-aminoalcohol quinoline enantiomers and investigation of the presumed underlying mechanism of action

    PubMed Central

    2012-01-01

    Background A better anti-malarial efficiency and lower neurotoxicity have been reported for mefloquine (MQ) (+)- enantiomer. However, the importance of stereoselectivity remains poorly understood as the anti-malarial activity of pure enantiomer MQ analogues has never been described. Building on these observations, a series of enantiopure 4-aminoalcohol quinoline derivatives has previously been synthesized to optimize the efficiency and reduce possible adverse effects. Their in vitro activity on Plasmodium falciparum W2 and 3D7 strains is reported here along with their inhibition of β-haematin formation and peroxidative degradation of haemin, two possible mechanisms of action of anti-malarial drugs. Results The (S)-enantiomers of this series of 4-aminoalcohol quinoline derivatives were found to be at least as effective as both chloroquine (CQ) and MQ. The derivative with a 5-carbon side-chain length was the more efficient on both P. falciparum strains. (R )-enantiomers displayed an activity decreased by 2 to 15-fold as compared to their (S) counterparts. The inhibition of β-haematin formation was significantly stronger with all tested compounds than with MQ, irrespective of the stereochemistry. Similarly, the inhibition of haemin peroxidation was significantly higher for both (S) and (R)-enantiomers of derivatives with a side-chain length of five or six carbons than for MQ and CQ. Conclusions The prominence of stereochemistry in the anti-malarial activity of 4-aminoalcohol quinoline derivatives is confirmed. The inhibition of β-haematin formation and haemin peroxidation can be put forward as presumed mechanisms of action but do not account for the stereoselectivity of action witnessed in vitro. PMID:22401346

  5. Gastroprotective activity of isopulegol on experimentally induced gastric lesions in mice: investigation of possible mechanisms of action.

    PubMed

    Silva, Maria Izabel Gomes; Moura, Brinell Arcanjo; Neto, Manuel Rufino de Aquino; Tomé, Adriana da Rocha; Rocha, Nayrton Flávio Moura; de Carvalho, Alyne Mara Rodrigues; Macêdo, Danielle Silveira; Vasconcelos, Silvânia Maria Mendes; de Sousa, Damião Pergentino; Viana, Glauce Socorro de Barros; de Sousa, Francisca Cléa Florenço

    2009-09-01

    The present study investigated whether isopulegol, a monoterpene present in essential oils of several aromatic plants, would be able to promote some gastroprotective effect and also verified the possible mechanisms involved in this action. For this study, ethanol- and indomethacin-induced gastric ulcer models in mice and histopathological assessment were used. The roles of NO, sulfhydryls (glutathione, GSH), ATP-sensitive K(+) channels (K(ATP) channels), and prostaglandins were also investigated. Isopulegol exhibited a dose-related gastroprotective effect against ethanol-induced lesions, while the pretreatment with glibenclamide and indomethacin [but not with N(G)-nitro-L-arginine methyl ester] were able to reverse this action. The pretreatment with isopulegol also restored GSH levels to normal levels and exhibited dose-related gastroprotective effect against indomethacin-induced ulcer. The results suggested that isopulegol presents significant gastroprotective effects in both ethanol- and indomethacin-induced ulcer models, which appear to be mediated, at least in part, by endogenous prostaglandins, K(ATP) channel opening, and antioxidant properties. PMID:19479241

  6. Clean slate corrective action investigation plan

    SciTech Connect

    1996-05-01

    The Clean Slate sites discussed in this report are situated in the central portion of the Tonopah Test Range (TTR), north of the Nevada Test Site (NTS) on the northwest portion of the Nellis Air Force Range (NAFR) which is approximately 390 kilometers (km) (240 miles [mi]) northwest of Las Vegas, Nevada. These sites were the locations for three of the four Operation Roller Coaster experiments. These experiments evaluated the dispersal of plutonium in the environment from the chemical explosion of a plutonium-bearing device. Although it was not a nuclear explosion, Operation Roller Coaster created some surface contamination which is now the subject of a corrective action strategy being implemented by the Nevada Environmental Restoration Project (NV ERP) for the U.S. Department of Energy (DOE). Corrective Action Investigation (CAI) activities will be conducted at three of the Operation Roller Coaster sites. These are Clean Slate 1 (CS-1), Clean Slate 2 (CS-2), and Clean Slate 3 (CS-3) sites, which are located on the TTR. The document that provides or references all of the specific information relative to the various investigative processes is called the Corrective Action Investigation Plan (CAIP). This CAIP has been prepared for the DOE Nevada Operations Office (DOE/NV) by IT Corporation (IT).

  7. Moving mirrors: a high-density EEG study investigating the effect of camera movements on motor cortex activation during action observation.

    PubMed

    Heimann, Katrin; Umiltà, Maria Alessandra; Guerra, Michele; Gallese, Vittorio

    2014-09-01

    Action execution-perception links (mirror mechanism) have been repeatedly suggested to play crucial roles in social cognition. Remarkably, the designs of most studies exploring this topic so far excluded even the simplest traces of social interaction, such as a movement of the observer toward another individual. This study introduces a new design by investigating the effects of camera movements, possibly simulating the observer's own approaching movement toward the scene. We conducted a combined high-density EEG and behavioral study investigating motor cortex activation during action observation measured by event-related desynchronization and resynchronization (ERD/ERS) of the mu rhythm. Stimuli were videos showing a goal-related hand action filmed while using the camera in four different ways: filming from a fixed position, zooming in on the scene, approaching the scene by means of a dolly, and approaching the scene by means of a steadycam. Results demonstrated a consistently stronger ERD of the mu rhythm for videos that were filmed while approaching the scene with a steadycam. Furthermore, videos in which the zoom was applied reliably demonstrated a stronger rebound. A rating task showed that videos in which the camera approached the scene were felt as more involving and the steadycam was most able to produce a visual experience close to the one of a human approaching the scene. These results suggest that filming technique predicts time course specifics of ERD/ERS during action observation with only videos simulating the natural vision of a walking human observer eliciting a stronger ERD than videos filmed from a fixed position. This demonstrates the utility of ecologically designed studies for exploring social cognition. PMID:24666130

  8. 14 CFR 13.129 - Post-investigation action.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Post-investigation action. 13.129 Section 13.129 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PROCEDURAL... Investigation § 13.129 Post-investigation action. A decision on whether to initiate subsequent action shall...

  9. 14 CFR 13.129 - Post-investigation action.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Post-investigation action. 13.129 Section 13.129 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PROCEDURAL... Investigation § 13.129 Post-investigation action. A decision on whether to initiate subsequent action shall...

  10. 14 CFR 13.129 - Post-investigation action.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Post-investigation action. 13.129 Section 13.129 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PROCEDURAL... Investigation § 13.129 Post-investigation action. A decision on whether to initiate subsequent action shall...

  11. Gastroprotective Activity of Violacein Isolated from Chromobacterium violaceum on Indomethacin-Induced Gastric Lesions in Rats: Investigation of Potential Mechanisms of Action

    PubMed Central

    Antonisamy, Paulrayer; Kannan, Ponnusamy; Aravinthan, Adithan; Duraipandiyan, Veeramuthu; Valan Arasu, Mariadhas; Ignacimuthu, Savarimuthu; Abdullah Al-Dhabi, Naif; Kim, Jong-Hoon

    2014-01-01

    Chromobacterium violaceum, Gram-negative bacteria species found in tropical regions of the world, produces a distinct deep violet-colored pigment called violacein. In the present study, we investigated whether violacein can promote a gastroprotective effect and verified the possible mechanisms involved in this action. For this study, an indomethacin-induced gastric ulcer rat model was used. The roles of biomolecules such as MPO, PGE2, pro- and anti-inflammatory cytokines, growth factors, caspase-3, NO, K+ATP channels, and α2-receptors were investigated. Violacein exhibited significant gastroprotective effect against indomethacin-induced lesions, while pretreatment with L-NAME and glibenclamide (but not with NEM or yohimbine) was able to reverse this action. Pretreatment with violacein also restored cNOS level to normal and led to attenuation of enhanced apoptosis and gastric microvascular permeability. Our results suggest that violacein provides a significant gastroprotective effect in an indomethacin-induced ulcer model through the maintenance of some vital protein molecules, and this effect appears to be mediated, at least in part, by endogenous prostaglandins, NOS, K+ATP channel opening, and inhibition of apoptosis and gastric microvascular permeability. PMID:25162059

  12. Copper(II) complexes with naringenin and hesperetin: cytotoxic activity against A 549 human lung adenocarcinoma cells and investigation on the mode of action.

    PubMed

    Tamayo, Lenka V; Gouvea, Ligiane R; Sousa, Anna C; Albuquerque, Ronniel M; Teixeira, Sarah Fernandes; de Azevedo, Ricardo Alexandre; Louro, Sonia R W; Ferreira, Adilson Kleber; Beraldo, Heloisa

    2016-02-01

    Copper(II) complexes [Cu(H2O)2 (L1)(phen)](ClO4) (1) and [Cu(H2O)(L2)(phen)](ClO4) (2) (HL1 = naringenin; HL2 = hesperetin) were obtained, in which an anionic flavonoid ligand is attached to the metal center along with 1,10-phenanthroline (phen) as co-ligand. Complexes (1) and (2) were assayed for their cytotoxic activity against A549 lung carcinoma and against normal lung fibroblasts (LL-24) and human umbilical vein endothelial cells (HUVEC). We found IC50 = 16.42 µM (1) and IC50 = 5.82 µM (2) against A549 tumor cells. Complexes (1) and (2) exhibited slight specificity, being more cytotoxic against malignant than against non-malignant cells. 1 and 2 induced apoptosis on A549 cells in a mitochondria-independent pathway, and showed antioxidant activity. The antioxidant effect of the complexes could possibly improve their apoptotic action, most likely by a PI3K-independent reduction of autophagy. Complexes (1) and (2) interact in vitro with calf thymus DNA by an intercalative binding mode. EPR data indicated that 1 and 2 interact with human serum albumin (HSA) forming mixed ligand species.

  13. Corrective Action Investigation Plan for Corrective Action Unit 554: Area 23 Release Site, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    David A. Strand

    2004-10-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information for conducting site investigation activities at Corrective Action Unit (CAU) 554: Area 23 Release Site, Nevada Test Site, Nevada. Information presented in this CAIP includes facility descriptions, environmental sample collection objectives, and criteria for the selection and evaluation of environmental samples. Corrective Action Unit 554 is located in Area 23 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 554 is comprised of one Corrective Action Site (CAS), which is: 23-02-08, USTs 23-115-1, 2, 3/Spill 530-90-002. This site consists of soil contamination resulting from a fuel release from underground storage tanks (USTs). Corrective Action Site 23-02-08 is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation prior to evaluating corrective action alternatives and selecting the appropriate corrective action for this CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document for CAU 554. Corrective Action Site 23-02-08 will be investigated based on the data quality objectives (DQOs) developed on July 15, 2004, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; and contractor personnel. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 554.

  14. Inquiry in Action: Investigating Matter through Inquiry.

    ERIC Educational Resources Information Center

    Kessler, James H.; Galvan, Patricia M.

    This book gives elementary and middle school teachers a set of physical science activities to help teach major concepts in the study of matter. The activities were developed to lend themselves to a guided inquiry approach for use with grades 3-8. To be effective over such a wide grade range, activities are designed to cover basic concepts but have…

  15. Corrective Action Investigation Plan for Corrective Action Unit 145: Wells and Storage Holes, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    David A. Strand

    2004-09-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information for conducting site investigation activities at Corrective Action Unit (CAU) 145: Wells and Storage Holes. Information presented in this CAIP includes facility descriptions, environmental sample collection objectives, and criteria for the selection and evaluation of environmental samples. Corrective Action Unit 145 is located in Area 3 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 145 is comprised of the six Corrective Action Sites (CASs) listed below: (1) 03-20-01, Core Storage Holes; (2) 03-20-02, Decon Pad and Sump; (3) 03-20-04, Injection Wells; (4) 03-20-08, Injection Well; (5) 03-25-01, Oil Spills; and (6) 03-99-13, Drain and Injection Well. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation (CAI) prior to evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. One conceptual site model with three release scenario components was developed for the six CASs to address all releases associated with the site. The sites will be investigated based on data quality objectives (DQOs) developed on June 24, 2004, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQOs process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 145.

  16. Investigating and Evaluating Environmental Issues and Actions: Skill Development Modules.

    ERIC Educational Resources Information Center

    Hungerford, Harold R.; And Others

    Middle school and high school students need to develop many skills in order to investigate environmental issues and know the basics of citizenship action strategies. This student guide is designed to teach students how to investigate and evaluate environmental issues and actions. The guide is presented in six modules. Module 1 introduces the…

  17. Corrective Action Investigation Plan for Corrective Action Unit 552: Area 12 Muckpile and Ponds, Nevada Test Site, Nevada: Revision 0

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2004-04-06

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach for collecting the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 552: Area 12 Muckpile and Ponds, Nevada Test Site (NTS), Nevada, under the Federal Facility Agreement and Consent Order. Located in Area 12 on the NTS, CAU 552 consists of two Corrective Action Sites (CASs): 12-06-04, Muckpile; 12-23-05, Ponds. Corrective Action Site 12-06-04 in Area 12 consists of the G-Tunnel muckpile, which is the result of tunneling activities. Corrective Action Site 12-23-05 consists of three dry ponds adjacent to the muckpile. The toe of the muckpile extends into one of the ponds creating an overlap of two CASs. The purpose of the investigation is to ensure that adequate data are collected to provide sufficient and reliable information to identify, evaluate, and select technic ally viable corrective actions. The results of the field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  18. Corrective Action Investigation plan for Corrective Action Unit 263: Area 25 Building 4839 Leachfield, Nevada Test Site, Nevada, March 1999

    SciTech Connect

    ITLV

    1999-03-01

    The Corrective Action Investigation Plan for Corrective Action Unit 263, the Area 25 Building 4839 Leachfield, has been developed in accordance with the Federal Facility Agreement and Consent Order that was agreed to by the US Department of Energy, Nevada Operations Office; the Nevada Division of Environmental Protection; and the US Department of Defense. Corrective Action Unit 263 is comprised of the Corrective Action Site 25-05-04 sanitary leachfield and associated collection system. This Corrective Action Investigation Plan is used in combination with the Work Plan for Leachfield Corrective Action Units: Nevada Test Site and Tonopah Test Range, Nevada (DOE/NV, 1998d). The Leachfield Work Plan was developed to streamline investigations at Leachfield Corrective Action Units by incorporating management, technical, quality assurance, health and safety, public involvement, field sampling, and waste management information common to a set of Corrective Action Units with similar site histories and characteristics into a single document that can be referenced. This Corrective Action Investigation Plan provides investigative details specific to Corrective Action Unit 263. Corrective Action Unit 263 is located southwest of Building 4839, in the Central Propellant Storage Area. Operations in Building 4839 from 1968 to 1996 resulted in effluent releases to the leachfield and associated collection system. In general, effluent released to the leachfield consisted of sanitary wastewater from a toilet, urinal, lavatory, and drinking fountain located within Building 4839. The subsurface soils in the vicinity of the collection system and leachfield may have been impacted by effluent containing contaminants of potential concern generated by support activities associated with the Building 4839 operations.

  19. Corrective Action Investigation Plan for Corrective Action Unit 240: Area 25 Vehicle Washdown Nevada Test Site, Nevada

    SciTech Connect

    DOE /NV

    1999-01-25

    This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO, CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites (FFACO, 1996). Corrective Action Units consist of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at CAU 240, Area 25 Vehicle Washdown, which is located on the Nevada Test Site (NTS).

  20. Corrective Action Investigation Plan for Corrective Action Unit 232: Area 25 Sewage Lagoons Nevada Test Site, Nevada

    SciTech Connect

    DOE /NV Operations Office

    1999-05-01

    This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) (1996) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense. The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO, CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites. A CAU consists of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and criteria for conducting site investigation activities at CAU 232, Area 25 Sewage Lagoons. Corrective Action Unit 232 consists of CAS 25-03-01, Sewage Lagoon, located in Area 25 of the Nevada Test Site (NTS). The NTS is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1) (DOE/NV, 1996a). The Area 25 Sewage Lagoons (Figure 1-2) (IT, 1999b) are located approximately 0.3 mi south of the Test Cell 'C' (TCC) Facility and were used for the discharge of sanitary effluent from the TCC facility. For purposes of this discussion, this site will be referred to as either CAU 232 or the sewage lagoons.

  1. Antiseptics and Disinfectants: Activity, Action, and Resistance

    PubMed Central

    McDonnell, Gerald; Russell, A. Denver

    1999-01-01

    Antiseptics and disinfectants are extensively used in hospitals and other health care settings for a variety of topical and hard-surface applications. A wide variety of active chemical agents (biocides) are found in these products, many of which have been used for hundreds of years, including alcohols, phenols, iodine, and chlorine. Most of these active agents demonstrate broad-spectrum antimicrobial activity; however, little is known about the mode of action of these agents in comparison to antibiotics. This review considers what is known about the mode of action and spectrum of activity of antiseptics and disinfectants. The widespread use of these products has prompted some speculation on the development of microbial resistance, in particular whether antibiotic resistance is induced by antiseptics or disinfectants. Known mechanisms of microbial resistance (both intrinsic and acquired) to biocides are reviewed, with emphasis on the clinical implications of these reports. PMID:9880479

  2. Corrective action investigation plan for the Roller Coaster RADSAFE Area, Corrective Action Unit 407, Tonopah Test Range, Nevada

    SciTech Connect

    1998-04-01

    This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO (1996), CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites. CAUs consist of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at CAU No. 407, the Roller Coaster RADSAFE Area (RCRSA) which is located on the Tonopah Test Range (TTR). The TTR, included in the Nellis Air Force Range Complex, is approximately 255 km (140 mi) northwest of Las Vegas, Nevada. CAU No. 407 is comprised of only one CAS (TA-23-001-TARC). The RCRSA was used during May and June 1963 to decontaminate vehicles, equipment, and personnel from the Clean Slate tests. The surface and subsurface soils are likely to have been impacted by plutonium and other contaminants of potential concern (COPCs) associated with decontamination activities at this site. The purpose of the corrective action investigation described in this CAIP is to: identify the presence and nature of COPCs; determine the vertical and lateral extent of COPCs; and provide sufficient information and data to develop and evaluate appropriate corrective actions for the CAS.

  3. Investigation of the low-level modulated light action

    NASA Astrophysics Data System (ADS)

    Antonov, Sergei N.; Sotnikov, V. N.; Koreneva, L. G.

    1994-07-01

    Now there exists no clear complete knowledge about mechanisms and pathways by which low level laser bioactivation works. Modulated laser light action has been investigated two new ways: dynamical infrared thermography and computing image of living brain. These ways permit observation in real time laser action on peripheral blood flow, reflex reactions to functional probes, thermoregulation mechanisms as well as brain electrical activity changes of humans. We have designed a universal apparatus which produced all regimes of the output laser light. It has a built-in He-Ne laser with an acousto-optic modulator and an infrared GaAs laser. The device provided spatial combination of both the light beams and permitted us to irradiate an object both separately and simultaneously. This research shows that the most effective frequencies range from several to dozens of hertz. The duty factor and frequency scanning are also important. On the basis of these results in Russian clinics new treatment methods using modulated light are applied in practical neurology, gynecology, etc.

  4. Corrective Action Investigation Plan for Corrective Action Unit 137: Waste Disposal Sites, Nevada Test Site, Nevada, Rev. No.:0

    SciTech Connect

    Wickline, Alfred

    2005-12-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 137: Waste Disposal Sites. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. Corrective Action Unit 137 contains sites that are located in Areas 1, 3, 7, 9, and 12 of the Nevada Test Site (NTS), which is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Corrective Action Unit 137 is comprised of the eight corrective action sites (CASs) shown on Figure 1-1 and listed below: (1) CAS 01-08-01, Waste Disposal Site; (2) CAS 03-23-01, Waste Disposal Site; (3) CAS 03-23-07, Radioactive Waste Disposal Site; (4) CAS 03-99-15, Waste Disposal Site; (5) CAS 07-23-02, Radioactive Waste Disposal Site; (6) CAS 09-23-07, Radioactive Waste Disposal Site; (7) CAS 12-08-01, Waste Disposal Site; and (8) CAS 12-23-07, Waste Disposal Site. The Corrective Action Investigation (CAI) will include field inspections, radiological surveys, geophysical surveys, sampling of environmental media, analysis of samples, and assessment of investigation results, where appropriate. Data will be obtained to support corrective action alternative evaluations and waste management decisions. The CASs in CAU 137 are being investigated because hazardous and/or radioactive constituents may be present in concentrations that could potentially pose a threat to human health and the environment. Existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives for the CASs. Additional information will be generated by conducting a CAI before evaluating and selecting corrective action

  5. Vicarious motor activation during action perception: beyond correlational evidence

    PubMed Central

    Avenanti, Alessio; Candidi, Matteo; Urgesi, Cosimo

    2013-01-01

    Neurophysiological and imaging studies have shown that seeing the actions of other individuals brings about the vicarious activation of motor regions involved in performing the same actions. While this suggests a simulative mechanism mediating the perception of others' actions, one cannot use such evidence to make inferences about the functional significance of vicarious activations. Indeed, a central aim in social neuroscience is to comprehend how vicarious activations allow the understanding of other people's behavior, and this requires to use stimulation or lesion methods to establish causal links from brain activity to cognitive functions. In the present work, we review studies investigating the effects of transient manipulations of brain activity or stable lesions in the motor system on individuals' ability to perceive and understand the actions of others. We conclude there is now compelling evidence that neural activity in the motor system is critical for such cognitive ability. More research using causal methods, however, is needed in order to disclose the limits and the conditions under which vicarious activations are required to perceive and understand actions of others as well as their emotions and somatic feelings. PMID:23675338

  6. Corrective Action Investigation Plan for Corrective Action Unit 551: Area 12 Muckpiles, Nevada Test Site, Nevada, Rev. No. 0

    SciTech Connect

    Robert F. Boehlecke

    2004-06-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 551, Area 12 muckpiles, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. Corrective Action Unit 551 is located in Area 12 of the NTS, which is approximately 110 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Area 12 is approximately 40 miles beyond the main gate to the NTS. Corrective Action Unit 551 is comprised of the four Corrective Action Sites (CASs) shown on Figure 1-1 and listed below: (1) 12-01-09, Aboveground Storage Tank and Stain; (2) 12-06-05, Muckpile; (3) 12-06-07, Muckpile; and (4) 12-06-08, Muckpile. Corrective Action Site 12-01-09 is located in Area 12 and consists of an above ground storage tank (AST) and associated stain. Corrective Action Site 12-06-05 is located in Area 12 and consists of a muckpile associated with the U12 B-Tunnel. Corrective Action Site 12-06-07 is located in Area 12 and consists of a muckpile associated with the U12 C-, D-, and F-Tunnels. Corrective Action Site 12-06-08 is located in Area 12 and consists of a muckpile associated with the U12 B-Tunnel. In keeping with common convention, the U12B-, C-, D-, and F-Tunnels will be referred to as the B-, C-, D-, and F-Tunnels. The corrective action investigation (CAI) will include field inspections, radiological surveys, and sampling of media, where appropriate. Data will also be obtained to support waste management decisions.

  7. Corrective Action Investigation Plan for Corrective Action Unit 551: Area 12 Muckpiles, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    David A. Strand

    2004-06-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 551, Area 12 muckpiles, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. Corrective Action Unit 551 is located in Area 12 of the NTS, which is approximately 110 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Area 12 is approximately 40 miles beyond the main gate to the NTS. Corrective Action Unit 551 is comprised of the four Corrective Action Sites (CASs) shown on Figure 1-1 and listed below: (1) 12-01-09, Aboveground Storage Tank and Stain; (2) 12-06-05, Muckpile; (3) 12-06-07, Muckpile; and (4) 12-06-08, Muckpile. Corrective Action Site 12-01-09 is located in Area 12 and consists of an above ground storage tank (AST) and associated stain. Corrective Action Site 12-06-05 is located in Area 12 and consists of a muckpile associated with the U12 B-Tunnel. Corrective Action Site 12-06-07 is located in Area 12 and consists of a muckpile associated with the U12 C-, D-, and F-Tunnels. Corrective Action Site 12-06-08 is located in Area 12 and consists of a muckpile associated with the U12 B-Tunnel. In keeping with common convention, the U12B-, C-, D-, and F-Tunnels will be referred to as the B-, C-, D-, and F-Tunnels. The corrective action investigation (CAI) will include field inspections, radiological surveys, and sampling of media, where appropriate. Data will also be obtained to support waste management decisions.

  8. Corrective Action Investigation Plan for Corrective Action Unit 555: Septic Systems Nevada Test Site, Nevada, Rev. No.: 0 with Errata

    SciTech Connect

    Pastor, Laura

    2005-12-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 555: Septic Systems, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. Corrective Action Unit 555 is located in Areas 1, 3 and 6 of the NTS, which is approximately 65 miles (mi) northwest of Las Vegas, Nevada, and is comprised of the five corrective action sites (CASs) shown on Figure 1-1 and listed below: (1) CAS 01-59-01, Area 1 Camp Septic System; (2) CAS 03-59-03, Core Handling Building Septic System; (3) CAS 06-20-05, Birdwell Dry Well; (4) CAS 06-59-01, Birdwell Septic System; and (5) CAS 06-59-02, National Cementers Septic System. An FFACO modification was approved on December 14, 2005, to include CAS 06-20-05, Birdwell Dry Well, as part of the scope of CAU 555. The work scope was expanded in this document to include the investigation of CAS 06-20-05. The Corrective Action Investigation (CAI) will include field inspections, radiological surveys, geophysical surveys, sampling of environmental media, analysis of samples, and assessment of investigation results, where appropriate. Data will be obtained to support corrective action alternative evaluations and waste management decisions. The CASs in CAU 555 are being investigated because hazardous and/or radioactive constituents may be present in concentrations that could potentially pose a threat to human health and the environment. Existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives for the CASs. Additional information will be generated by conducting a CAI

  9. Corrective action investigation plan: Cactus Spring Waste Trenches. Revision 2

    SciTech Connect

    1997-02-01

    This Correction Action Investigation Plan (CAIP) contains environmental sample collection objectives and logic for the CAU No. 426, which includes the Cactus Spring Waste Trenches, CAS No. RG-08-001-RG-CS. The Cactus Spring Waste Trenches are located at the Tonopah Test Range (TTR) which is part of the Nellis Air Force Range, approximately 255 kilometers (km) (140 miles [mi]) northwest of Las Vegas, Nevada, by air. The purpose of this investigation is to generate sufficient data to establish the types of waste buried in the trenches, identify the presence and nature of contamination, determine the vertical extent of contaminant migration below the Cactus Spring Waste Trenches, and determine the appropriate course of action for the site. The potential courses of action for the site are clean closure, closure in place (with or without remediation), or no further action.

  10. Fungistatic activity of Zanthoxylum rhoifolium Lam. bark extracts against fungal plant pathogens and investigation on mechanism of action in Botrytis cinerea.

    PubMed

    Carotenuto, Gennaro; Carrieri, Raffaele; Tarantino, Paola; Alfieri, Mariaevelina; Leone, Antonella; De Tommasi, Nunziatina; Lahoz, Ernesto

    2015-01-01

    Plant-derived compounds are emerging as an alternative choice to synthetic fungicides. Chloroform-methanol extract, obtained from the bark of Zanthoxylum rhoifolium, a member of Rutaceae, showed a fungistatic effect on Botrytis cinerea, Sclerotinia sclerotiorum, Alternaria alternata, Colletotrichum gloeosporioides and Clonostachys rosea, when added to the growth medium at different concentrations. A fraction obtained by gel separation and containing the alkaloid O-Methylcapaurine showed significant fungistatic effect against B. cinerea and S. sclerotiorum, two of the most destructive phytopathogenic fungi. The underlying mechanism of such an inhibition was further investigated in B. cinerea, a fungus highly prone to develop fungicide resistance, by analysing the expression levels of a set of genes (BcatrB, P450, CYP51 and TOR). O-Methylcapaurine inhibited the expression of all the analysed genes. In particular, the expression of BcatrB gene, encoding a membrane drug transporter involved in the resistance to a wide range of xenobiotic compounds, was strongly inhibited (91%).

  11. Corrective action investigation plan for CAU Number 453: Area 9 Landfill, Tonopah Test Range

    SciTech Connect

    1997-05-14

    This Corrective Action Investigation Plan (CAIP) contains the environmental sample collection objectives and criteria for conducting site investigation activities at the Area 9 Landfill, Corrective Action Unit (CAU) 453/Corrective Action (CAS) 09-55-001-0952, which is located at the Tonopah Test Range (TTR). The TTR, included in the Nellis Air Force Range, is approximately 255 kilometers (140 miles) northwest of Las Vegas, Nevada. The Area 9 Landfill is located northwest of Area 9 on the TTR. The landfill cells associated with CAU 453 were excavated to receive waste generated from the daily operations conducted at Area 9 and from range cleanup which occurred after test activities.

  12. Corrective Action Investigation Plan for Corrective Action Unit 528: Polychlorinated Biphenyls Contamination, Nevada Test Site, Nevada, Rev. 0

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2003-05-08

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 528, Polychlorinated Biphenyls Contamination (PCBs), Nevada Test Site (NTS), Nevada, under the Federal Facility Agreement and Consent Order. Located in the southwestern portion of Area 25 on the NTS in Jackass Flats (adjacent to Test Cell C [TCC]), CAU 528 consists of Corrective Action Site 25-27-03, Polychlorinated Biphenyls Surface Contamination. Test Cell C was built to support the Nuclear Rocket Development Station (operational between 1959 and 1973) activities including conducting ground tests and static firings of nuclear engine reactors. Although CAU 528 was not considered as a direct potential source of PCBs and petroleum contamination, two potential sources of contamination have nevertheless been identified from an unknown source in concentrations that could potentially pose an unacceptable risk to human health and/or the environment. This CAU's close proximity to TCC prompted Shaw to collect surface soil samples, which have indicated the presence of PCBs extending throughout the area to the north, east, south, and even to the edge of the western boundary. Based on this information, more extensive field investigation activities are being planned, the results of which are to be used to support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  13. Mechanism of action of platelet activating factor in the pulmonary circulation: an investigation using a novel isotopic system in rabbit isolated lung.

    PubMed Central

    Seale, J. P.; Nourshargh, S.; Hellewell, P. G.; Williams, T. J.

    1991-01-01

    1. Rabbit isolated lungs were perfused via the pulmonary artery with Tyrode solution containing 4.5% Ficoll and 0.1% bovine serum albumin at a constant rate of 20 ml min-1. Lung perfusate was drawn for alternating 5 min periods from two reservoirs, one containing 125I-albumin and the other unlabelled albumin to wash out the intravascular label. Microvascular 125I-albumin leakage was determined from the count remaining at the end of the washout phase with an external gamma scintillation probe. In addition, perfusion pressure was monitored continuously. Each experiment comprised 6 cycles over a total period of 60 min. 2. Infusion of platelet activating factor (PAF, 3 nmol min-1 for 10 min) resulted in microvascular 125I-albumin leakage, whereas lyso-PAF was without effect. During PAF infusions there was also an increase in perfusion pressure. Both the permeability and pressor effects of PAF were inhibited by the PAF antagonist L-652731. 3. Infusion of the thromboxane analogue U-46619 (0.6 nmol min-1 for 10 min) caused an increase in perfusion pressure but protein accumulation was not significantly different from that observed with control infusions. 4. Bolus injections of PAF (1 nmol) caused increases in perfusion pressure which were reduced by indomethacin, dazmegrel and BW 755C. Bolus injections of PAF, repeated at 30 min intervals caused reproducible pressor responses; however, repeated injections at 60 min intervals resulted in augmented responses. This augmentation did not occur in the presence of indomethacin. 5. Retrograde perfusion of PAF via the pulmonary vein induced increased perfusion pressure and microvascular 125I-albumin leakage.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1786514

  14. Active Sites Environmental Monitoring Program: Action levels

    SciTech Connect

    Ashwood, J.S.; Ashwood, T.L.

    1991-10-01

    The Active Sites Environmental Monitoring Program (ASEMP) was established at Oak Ridge National Laboratory to provide for early leak detection and to monitor performance of the active low-level waste disposal facilities in Solid Waste Storage Area (SWSA) 6 and the transuranic waste storage areas in SWSA 5 North. Early leak detection is accomplished by sampling runoff, groundwater, and perched water in burial trenches. Sample results are compared to action levels that represent background contamination by naturally occurring and fallout-derived radionuclides. 15 refs., 3 figs., 12 tabs.

  15. Corrective Action Investigation Plan for Corrective Action Unit 190: Contaminated Waste Sites Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    Wickline, Alfred

    2006-12-01

    Corrective Action Unit (CAU) 190 is located in Areas 11 and 14 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 190 is comprised of the four Corrective Action Sites (CASs) listed below: (1) 11-02-01, Underground Centrifuge; (2) 11-02-02, Drain Lines and Outfall; (3) 11-59-01, Tweezer Facility Septic System; and (4) 14-23-01, LTU-6 Test Area. These sites are being investigated because existing information is insufficient on the nature and extent of potential contamination to evaluate and recommend corrective action alternatives. Additional information will be obtained before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS by conducting a corrective action investigation (CAI). The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on August 24, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture, and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 190. The scope of the CAU 190 CAI includes the following activities: (1) Move surface debris and/or materials, as needed, to facilitate sampling; (2) Conduct radiological and geophysical surveys; (3) Perform field screening; (4) Collect and submit environmental samples for laboratory analysis to determine whether contaminants of concern (COCs) are present; (5) If COCs are present, collect additional step-out samples to define the lateral and vertical extent of the contamination; (6) Collect samples of source material, if present

  16. Change in Action: Navigating and Investigating the Classroom Using Action Research.

    ERIC Educational Resources Information Center

    Tonack, De, Ed.; Dean, Ceri, Ed.

    Thirty-five Nebraska educators from across the state came together for five Saturday seminars during the 1995-96 school year to collaborate as they conducted classroom investigations. They earned 3 hours of tuition-free graduate credit. Action research was the tool for their investigations, guided by R. Sagor's book "How To Conduct Collaborative…

  17. Corrective action investigation plan: Cactus Spring Waste Trenches. Revision 2

    SciTech Connect

    1997-02-01

    This Correction Action Investigation Plan (CAIP) contains environmental sample collection objectives and logic for the Corrective Action Unit No. 426, which includes the Cactus Spring Waste Trenches, located at the Tonopah Test Range. The purpose of this investigation is to generate sufficient data to establish the types of waste buried in the trenches, identify the presence and nature of contamination, determine the vertical extent of contaminant migration below the Cactus Spring Waste Trenches, and determine the appropriate course of action for the site. The potential courses of action for the site are clean closure, closure in place (with or without remediation), or no further action. The scope of this investigation will include drilling and collecting subsurface samples from within and below the trenches. Sampling locations will be biased toward the areas most likely to be contaminated. The Cactus Spring Waste Trenches Site is identified as one of three potential locations for buried, radioactively contaminated materials from the Double Tracks Test. This test was the first of four storage-transportation tests conducted in 1963 as part of Operation Roller Coaster. The experiment involved the use of live animals to assess the inhalation intake of a plutonium aerosol.

  18. Metal complexes of 3-(4-bromophenyl)-1-pyridin-2-ylprop-2-en-1-one thiosemicarbazone: cytotoxic activity and investigation on the mode of action of the gold(III) complex.

    PubMed

    Sâmia, Luciana B P; Parrilha, Gabrieli L; Da Silva, Jeferson G; Ramos, Jonas P; Souza-Fagundes, Elaine M; Castelli, Silvia; Vutey, Venn; Desideri, Alessandro; Beraldo, Heloisa

    2016-06-01

    Complexes [Au(PyCT4BrPh)Cl]Cl (1), [Pt(PyCT4BrPh)Cl]0.5KCl (2), and [Pd(PyCT4BrPh)Cl]KCl (3) were obtained with 3-(4-bromophenyl)-1-pyridin-2-ylprop-2-en-1-one thiosemicarbazone (HPyCT4BrPh). Although complexes (2) and (3) did not exhibit potent cytotoxic activity, HPyCT4BrPh and its gold(III) complex (1) proved to be highly cytotoxic against HL-60 (human promyelocytic leukemia) and THP-1 (human monocytic leukemia) cells, and against MDA-MB 231 and MCF-7 (human breast adenocarcinoma) solid tumor cells. Except for HL-60 cells, upon coordination to gold(III) a 2- to 3-fold increase in the cytotoxic effect was observed. An investigation on the possible biological targets of the gold(III) complex was carried out. Complex (1) but not the free thiosemicarbazone inhibits the enzymatic activity of thioredoxin reductase (TrxR). The affinity of 1 for TrxR suggests metal binding to a selenol residue in the active site of the enzyme. While HPyCT4BrPh was inactive, 1 was able to inhibit topoisomerase IB (Topo IB) activity. Hence, inhibition of TrxR and Topo IB could contribute to the mechanism of cytotoxic action of complex (1).

  19. Corrective Action Investigation Plan for Corrective Action Unit 565: Stored Samples, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    Wickline, Alfred; McCall, Robert

    2006-08-01

    Corrective Action Unit (CAU) 565 is located in Area 26 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 565 is comprised of one corrective action site (CAS) listed--CAS 26-99-04, Ground Zero Soil Samples. This site is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend closure of CAU 565. Additional information will be obtained by conducting a corrective action investigation before evaluating closure objectives and selecting the appropriate corrective action. The results of the field investigation will support closure and waste management decisions that will be presented in the Corrective Action Decision Document/Closure Report. The site will be investigated based on the data quality objectives (DQOs) developed on June 1, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQO process was utilized to identify and define the type, amount, and quality of data needed to develop and evaluate closure for CAU 565. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to this CAS. The scope of the corrective action investigation for CAU 565 includes the following activities: (1) Remove stored samples, shelves, and debris from the interior of Building 26-2106. (2) Perform field screening on stored samples, shelves, and debris. (3) Dispose of stored samples, shelves, and debris. (4) Collect samples of investigation-derived waste, as needed, for waste management purposes. (5) Conduct radiological surveys of Building 26-2106 in accordance with the requirements in the ''NV/YMP Radiological Control Manual'' to determine if there is residual radiological contamination that would prevent the release of the building for unrestricted use. This

  20. Using Action Research to Investigate Social Networking Technologies

    ERIC Educational Resources Information Center

    Worrall, Lisa; Harris, Katy

    2013-01-01

    This article outlines the first cycle of an Action Research (AR) investigation into why professional learners are not using the Social Networking Technologies (SNTs) of their bespoke website. It presents the rationale of how this study came about, the ontological and epistemological stance of the authors and how this led to the particular choice…

  1. Corrective Action Investigation Plan for Corrective Action Unit 557: Spills and Tank Sites, Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Alfred Wickline

    2008-07-01

    Corrective Action Unit (CAU) 557 is located in Areas 1, 3, 6, and 25 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada, and is comprised of the four corrective action sites (CASs) listed below: • 01-25-02, Fuel Spill • 03-02-02, Area 3 Subdock UST • 06-99-10, Tar Spills • 25-25-18, Train Maintenance Bldg 3901 Spill Site These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 3, 2008, by representatives of the Nevada Division of Environmental Protection (NDEP); U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 557. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the corrective action investigation for CAU 557 includes the following activities: • Move surface debris and/or materials, as needed, to facilitate sampling. • Conduct radiological survey at CAS 25-25-18. • Perform field screening. • Collect and submit environmental samples for laboratory analysis to determine whether contaminants of concern are present. • If contaminants of concern are present, collect additional step

  2. Corrective action investigation plan for Corrective Action Unit Number 427: Area 3 septic waste system numbers 2 and 6, Tonopah Test Range, Nevada

    SciTech Connect

    1997-09-19

    This Corrective Action Investigation Plan (CAIP) contains the environmental sample collection objectives and the criteria for conducting site investigation activities at the Area 3 Compound, specifically Corrective Action Unit (CAU) Number 427, which is located at the Tonopah Test Range (TTR). The TTR, included in the Nellis Air Force Range, is approximately 255 kilometers (140 miles) northwest of Las Vegas, Nevada. The Corrective Action Unit Work Plan, Tonopah Test Range, Nevada divides investigative activities at TTR into Source Groups. The Septic Tanks and Lagoons Group consists of seven CAUs. Corrective Action Unit Number 427 is one of three septic waste system CAUs in TTR Area 3. Corrective Action Unit Numbers 405 and 428 will be investigated at a future data. Corrective Action Unit Number 427 is comprised of Septic Waste Systems Number 2 and 6 with respective CAS Numbers 03-05-002-SW02 and 03-05-002-SW06.

  3. Corrective Action Investigation Plan for Corrective Action Unit 309: Area 12 Muckpiles, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    David A. Strand

    2004-12-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 309, Area 12 Muckpiles, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense (DoD). Corrective Action Unit 309 is located in Area 12 of the NTS, which is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Area 12 is approximately 40 mi beyond the main gate to the NTS. Corrective Action Unit 309 is comprised of the three Corrective Action Sites (CASs) shown on Figure 1-1 and listed below: CAS 12-06-09, Muckpile; CAS 12-08-02, Contaminated Waste Dump (CWD); and CAS 12-28-01, I, J, and K-Tunnel Debris. Corrective Action Sites 12-06-09 and 12-08-02 will be collectively referred to as muckpiles in this document. Corrective Action Site 12-28-01 will be referred to as the fallout plume because of the extensive lateral area of debris and fallout contamination resulting from the containment failures of the J-and K-Tunnels. The corrective action investigation (CAI) will include field inspections, radiological surveys, and media sampling, where appropriate. Data will also be obtained to support waste management decisions. The CASs in CAU 309 are being investigated because hazardous and/or radioactive constituents may be present in concentrations that could potentially pose a threat to human health and/or the environment. Existing information on the nature and extent of potential contamination at these sites are insufficient to evaluate and recommend corrective action alternatives for the CASs. Therefore, additional information will be obtained by conducting a CAI prior to evaluating corrective action

  4. Corrective action investigation plan for Corrective Action Unit 340, Pesticide Release Sites, Nevada Test Site, Nye County, Nevada

    SciTech Connect

    1998-01-01

    This Correction Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense. As required by the FFACO (1996), this document provides or references all of the specific information for planning investigation activities associated with three Corrective Action Sites (CASs) located at the Nevada Test Site (NTS). These CASs are collectively known as Corrective Action Unit (CAU) 340, Pesticide Release Sites. According to the FFACO, CASs are sites that may require corrective action(s) and may include solid waste management units or individual disposal or release sites. These sites are CAS 23-21-01, Area 23 Quonset Hut 800 (Q800) Pesticide Release Ditch; CAS 23-18-03, Area 23 Skid Huts Pesticide Storage; and CAS 15-18-02, Area 15 Quonset Hut 15-11 Pesticide Storage (Q15-11). The purpose of this CAIP for CAU 340 is to direct and guide the investigation for the evaluation of the nature and extent of pesticides, herbicides, and other contaminants of potential concern (COPCs) that were stored, mixed, and/or disposed of at each of the CASs.

  5. A Novel Task for the Investigation of Action Acquisition

    PubMed Central

    Stafford, Tom; Thirkettle, Martin; Walton, Tom; Vautrelle, Nicolas; Hetherington, Len; Port, Michael; Gurney, Kevin; Redgrave, Pete

    2012-01-01

    We present a behavioural task designed for the investigation of how novel instrumental actions are discovered and learnt. The task consists of free movement with a manipulandum, during which the full range of possible movements can be explored by the participant and recorded. A subset of these movements, the ‘target’, is set to trigger a reinforcing signal. The task is to discover what movements of the manipulandum evoke the reinforcement signal. Targets can be defined in spatial, temporal, or kinematic terms, can be a combination of these aspects, or can represent the concatenation of actions into a larger gesture. The task allows the study of how the specific elements of behaviour which cause the reinforcing signal are identified, refined and stored by the participant. The task provides a paradigm where the exploratory motive drives learning and as such we view it as in the tradition of Thorndike [1]. Most importantly it allows for repeated measures, since when a novel action is acquired the criterion for triggering reinforcement can be changed requiring a new action to be discovered. Here, we present data using both humans and rats as subjects, showing that our task is easily scalable in difficulty, adaptable across species, and produces a rich set of behavioural measures offering new and valuable insight into the action learning process. PMID:22675490

  6. Corrective Action Investigation Plan for Corrective Action Unit 321: Area 22 Weather Station Fuel Storage, Nevada Test Site, Nevada

    SciTech Connect

    DOE /NV

    1999-01-28

    This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO (1996), CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites. A CAU consists of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at the CAU 321 Area 22 Weather Station Fuel Storage, CAS 22-99-05 Fuel Storage Area. For purposes of this discussion, this site will be referred to as either CAU 321 or the Fuel Storage Area. The Fuel Storage Area is located in Area 22 of the Nevada Test Site (NTS). The NTS is approximately 105 kilometers (km) (65 miles [mi]) northwest of Las Vegas, Nevada (Figure 1-1) (DOE/NV, 1996a). The Fuel Storage Area (Figure 1-2) was used to store fuel and other petroleum products necessary for motorized operations at the historic Camp Desert Rock facility which was operational from 1951 to 1958 at the Nevada Test Site, Nevada. The site was dismantled after 1958 (DOE/NV, 1996a).

  7. Corrective Action Investigation Plan for Corrective Action Unit 486: Double Tracks RADSAFE Area Nellis Air Force Range, Nevada

    SciTech Connect

    IT Las Vegas

    1998-10-15

    This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO, CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites (FFACO, 1996). Corrective Action Units consist of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at CAU 486, the Double Tracks Radiological Safety (RADSAFE) Area (DTRSA) which is located on the Nellis Air Force Range 71North (N), west of the Tonopah Test Range (TTR). The TTR, included in the Nellis Air Force Range Complex, is approximately 255 kilometers (km) (140 miles [mi]) northwest of Las Vegas, Nevada (Figure 1-1). Corrective Action Unit 486 is comprised of CAS 71-23-001-71DT consisting of two areas of concern referred to as the vehicle decontamination area and the animal burial pit. The DTRSA is located on the west side of the Cactus Range approximately 8 km (5 mi) southwest of the Cactus Spring gate at the intersection of the Cactus Spring Road and the Double Tracks Control Point Road (Figure 1-2). The DTRSA was used during May 1963 to decontaminate vehicles, equipment, personnel, and animals from the Double Tracks test. The DTRSA is one of three areas identified as a potential location for the disposal of radioactively contaminated

  8. Corrective Action Investigation Plan for Corrective Action Unit 562: Waste Systems Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Alfred Wickline

    2009-04-01

    Corrective Action Unit 562 is located in Areas 2, 23, and 25 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 562 is comprised of the 13 corrective action sites (CASs) listed below: • 02-26-11, Lead Shot • 02-44-02, Paint Spills and French Drain • 02-59-01, Septic System • 02-60-01, Concrete Drain • 02-60-02, French Drain • 02-60-03, Steam Cleaning Drain • 02-60-04, French Drain • 02-60-05, French Drain • 02-60-06, French Drain • 02-60-07, French Drain • 23-60-01, Mud Trap Drain and Outfall • 23-99-06, Grease Trap • 25-60-04, Building 3123 Outfalls These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on December 11, 2008, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 562. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the corrective action investigation for CAU 562 includes the following activities: • Move surface debris and/or materials, as needed, to facilitate sampling.

  9. Corrective Action Investigation Plan for Corrective Action Unit 543: Liquid Disposal Units, Nevada Test Site, Nevada: Revision 0

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2004-05-03

    The general purpose of this Corrective Action Investigation Plan is to ensure that adequate data are collected to provide sufficient and reliable information to identify, evaluate, and select technically viable corrective action alternatives (CAAs) for Corrective Action Unit (CAU) 543: Liquid Disposal Units, Nevada Test Site (NTS), Nevada. Located in Areas 6 and 15 on the NTS, CAU 543 is comprised of a total of seven corrective action sites (CASs), one in Area 6 and six in Area 15. The CAS in Area 6 consists of a Decontamination Facility and its components which are associated with decontamination of equipment, vehicles, and materials related to nuclear testing. The six CASs in Area 15 are located at the U.S. Environmental Protection Agency Farm and are related to waste disposal activities at the farm. Sources of possible contamination at Area 6 include potentially contaminated process waste effluent discharged through a process waste system, a sanitary waste stream generated within buildings of the Decon Facility, and radiologically contaminated materials stored within a portion of the facility yard. At Area 15, sources of potential contamination are associated with the dairy operations and the animal tests and experiments involving radionuclide uptake. Identified contaminants of potential concern include volatile organic compounds, semivolatile organic compounds, petroleum hydrocarbons, pesticides, herbicides, polychlorinated biphenyls, metals, and radionuclides. Three corrective action closure alternatives - No Further Action, Close in Place, or Clean Closure - will be recommended for CAU 543 based on an evaluation of all the data quality objective-related data. Field work will be conducted following approval of the plan. The results of the field investigation will support a defensible evaluation of CAAs that will be presented in the Corrective Action Decision Document.

  10. Corrective Action Investigation Plan for Corrective Action Unit 542: Disposal Holes, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    Laura Pastor

    2006-05-01

    Corrective Action Unit (CAU) 542 is located in Areas 3, 8, 9, and 20 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 542 is comprised of eight corrective action sites (CASs): (1) 03-20-07, ''UD-3a Disposal Hole''; (2) 03-20-09, ''UD-3b Disposal Hole''; (3) 03-20-10, ''UD-3c Disposal Hole''; (4) 03-20-11, ''UD-3d Disposal Hole''; (5) 06-20-03, ''UD-6 and UD-6s Disposal Holes''; (6) 08-20-01, ''U-8d PS No.1A Injection Well Surface Release''; (7) 09-20-03, ''U-9itsy30 PS No.1A Injection Well Surface Release''; and (8) 20-20-02, ''U-20av PS No.1A Injection Well Surface Release''. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on January 30, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 542. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the CAI for CAU 542 includes the following activities: (1) Move surface debris and/or materials, as needed, to facilitate sampling. (2) Conduct radiological surveys. (3) Conduct geophysical surveys to

  11. AMPK activators: mechanisms of action and physiological activities.

    PubMed

    Kim, Joungmok; Yang, Goowon; Kim, Yeji; Kim, Jin; Ha, Joohun

    2016-01-01

    AMP-activated protein kinase (AMPK) is a central regulator of energy homeostasis, which coordinates metabolic pathways and thus balances nutrient supply with energy demand. Because of the favorable physiological outcomes of AMPK activation on metabolism, AMPK has been considered to be an important therapeutic target for controlling human diseases including metabolic syndrome and cancer. Thus, activators of AMPK may have potential as novel therapeutics for these diseases. In this review, we provide a comprehensive summary of both indirect and direct AMPK activators and their modes of action in relation to the structure of AMPK. We discuss the functional differences among isoform-specific AMPK complexes and their significance regarding the development of novel AMPK activators and the potential for combining different AMPK activators in the treatment of human disease. PMID:27034026

  12. AMPK activators: mechanisms of action and physiological activities

    PubMed Central

    Kim, Joungmok; Yang, Goowon; Kim, Yeji; Kim, Jin; Ha, Joohun

    2016-01-01

    AMP-activated protein kinase (AMPK) is a central regulator of energy homeostasis, which coordinates metabolic pathways and thus balances nutrient supply with energy demand. Because of the favorable physiological outcomes of AMPK activation on metabolism, AMPK has been considered to be an important therapeutic target for controlling human diseases including metabolic syndrome and cancer. Thus, activators of AMPK may have potential as novel therapeutics for these diseases. In this review, we provide a comprehensive summary of both indirect and direct AMPK activators and their modes of action in relation to the structure of AMPK. We discuss the functional differences among isoform-specific AMPK complexes and their significance regarding the development of novel AMPK activators and the potential for combining different AMPK activators in the treatment of human disease. PMID:27034026

  13. Corrective Action Investigation Plan for Corrective Action Unit 447: Project Shoal Area, Nevada Subsurface Site

    SciTech Connect

    DOE /NV

    1998-11-01

    This Corrective Action Investigation Plan (CAIP) describes the US Department of Energy's (DOE's) continued environmental investigation of the subsurface Project Shoal Area (PSA) Corrective Action Unit (CAU) 447. The PSA is located in the Sand Springs Mountains in Churchill County, Nevada, about 48 kilometers (km) (30 miles [mi]) southeast of Fallon, Nevada. Project Shoal was part of the Vela Uniform Program which was conducted to improve the US' ability to detect, identify, and locate underground nuclear detonations. The test consisted of detonating a 12-kiloton nuclear device deep underground in granitic rock to determine whether seismic waves produced by an underground nuclear test could be differentiated from seismic waves produced by a naturally occurring earthquake. The test was a joint effort conducted by the US Atomic Energy Commission (AEC) and the US Department of Defense (DoD) in October 1963 (AEC, 1964).

  14. Corrective Action Investigation Plan for Corrective Action Unit 563: Septic Systems, Nevada Test Site, Nevada, with Errata Sheet, Revision 0

    SciTech Connect

    Alfred Wickline

    2007-01-01

    Corrective Action Unit 563, Septic Systems, is located in Areas 3 and 12 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 563 is comprised of the four corrective action sites (CASs) below: • 03-04-02, Area 3 Subdock Septic Tank • 03-59-05, Area 3 Subdock Cesspool • 12-59-01, Drilling/Welding Shop Septic Tanks • 12-60-01, Drilling/Welding Shop Outfalls These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document.

  15. Corrective Action Investigation Plan for Corrective Action Unit 487: Thunderwell Site, Tonopah Test Range, Nevada (Rev. No.: 0, January 2001)

    SciTech Connect

    DOE /NV

    2001-01-02

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operations Office's (DOE/NV's) approach to collect the data necessary to evaluate corrective action alternatives (CAAs) appropriate for the closure of Corrective Action Unit (CAU) 487, Thunderwell Site, Tonopah Test Range (TTR), Nevada, under the Federal Facility Agreement and Consent Order. Corrective Action Unit 487 consists of a single Corrective Action Site (CAS), RG 26-001-RGRV, Thunderwell Site. The site is located in the northwest portion of the TTR, Nevada, approximately five miles northwest of the Area 3 Control Point and closest to the Cactus Flats broad basin. Historically, Sandia National Laboratories in New Mexico used CAU 487 in the early to mid-1960s for a series of high explosive tests detonated at the bottom of large cylindrical steel tubes. Historical photographs indicate that debris from these tests and subsequent operations may have been scattered and buried throughout the site. A March 2000 walk-over survey and a July 2000 geophysical survey indicated evidence of buried and surface debris in dirt mounds and areas throughout the site; however, a radiological drive-over survey also performed in July 2000 indicated that no radiological hazards were identified at this site. Based on site history, the scope of this plan is to resolve the problem statement identified during the Data Quality Objectives process that detonation activities at this CAU site may have resulted in the release of contaminants of concern into the surface/subsurface soil including total volatile and total semivolatile organic compounds, total Resource Conservation and Recovery Act metals, radionuclides, total petroleum hydrocarbons, and high explosives. Therefore, the scope of corrective action field investigation will involve excavation, drilling, and extensive soil sampling and analysis activities to determine the extent (if any) of both the lateral and vertical contamination and whether

  16. Corrective Action Investigation Plan for Corrective Action Unit 550: Smoky Contamination Area Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Grant Evenson

    2012-05-01

    on January 31, 2012, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 550. The potential contamination sources associated with the study groups are from nuclear testing activities conducted at CAU 550. The DQO process resulted in an assumption that the total effective dose (TED) within the default contamination boundary of CAU 550 exceeds the final action level and requires corrective action. The presence and nature of contamination outside the default contamination boundary at CAU 550 will be evaluated based on information collected from a field investigation. Radiological contamination will be evaluated based on a comparison of the TED at sample locations to the dose-based final action level. The TED will be calculated as the total of separate estimates of internal and external dose. Results from the analysis of soil samples will be used to calculate internal radiological dose. Thermoluminescent dosimeters placed at the center of each sample location will be used to measure external radiological dose. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each group of CASs.

  17. Predictive motor activation during action observation in human infants.

    PubMed

    Southgate, Victoria; Johnson, Mark H; Osborne, Tamsin; Csibra, Gergely

    2009-12-23

    Certain regions of the human brain are activated both during action execution and action observation. This so-called 'mirror neuron system' has been proposed to enable an observer to understand an action through a process of internal motor simulation. Although there has been much speculation about the existence of such a system from early in life, to date there is little direct evidence that young infants recruit brain areas involved in action production during action observation. To address this question, we identified the individual frequency range in which sensorimotor alpha-band activity was attenuated in nine-month-old infants' electroencephalographs (EEGs) during elicited reaching for objects, and measured whether activity in this frequency range was also modulated by observing others' actions. We found that observing a grasping action resulted in motor activation in the infant brain, but that this activity began prior to observation of the action, once it could be anticipated. These results demonstrate not only that infants, like adults, display overlapping neural activity during execution and observation of actions, but that this activation, rather than being directly induced by the visual input, is driven by infants' understanding of a forthcoming action. These results provide support for theories implicating the motor system in action prediction. PMID:19675001

  18. Corrective Action Decision Document for Corrective Action Unit 417: Central Nevada Test Area Surface, Nevada Appendix D - Corrective Action Investigation Report, Central Nevada Test Area, CAU 417

    SciTech Connect

    U.S. Department of Energy, Nevada Operations office

    1999-04-02

    This Corrective Action Decision Document (CADD) identifies and rationalizes the U.S. Department of Energy, Nevada Operations Office's selection of a recommended corrective action alternative (CAA) appropriate to facilitate the closure of Corrective Action Unit (CAU) 417: Central Nevada Test Area Surface, Nevada, under the Federal Facility Agreement and Consent Order. Located in Hot Creek Valley in Nye County, Nevada, and consisting of three separate land withdrawal areas (UC-1, UC-3, and UC-4), CAU 417 is comprised of 34 corrective action sites (CASs) including 2 underground storage tanks, 5 septic systems, 8 shaker pad/cuttings disposal areas, 1 decontamination facility pit, 1 burn area, 1 scrap/trash dump, 1 outlier area, 8 housekeeping sites, and 16 mud pits. Four field events were conducted between September 1996 and June 1998 to complete a corrective action investigation indicating that the only contaminant of concern was total petroleum hydrocarbon (TPH) which was found in 18 of the CASs. A total of 1,028 samples were analyzed. During this investigation, a statistical approach was used to determine which depth intervals or layers inside individual mud pits and shaker pad areas were above the State action levels for the TPH. Other related field sampling activities (i.e., expedited site characterization methods, surface geophysical surveys, direct-push geophysical surveys, direct-push soil sampling, and rotosonic drilling located septic leachfields) were conducted in this four-phase investigation; however, no further contaminants of concern (COCs) were identified. During and after the investigation activities, several of the sites which had surface debris but no COCs were cleaned up as housekeeping sites, two septic tanks were closed in place, and two underground storage tanks were removed. The focus of this CADD was to identify CAAs which would promote the prevention or mitigation of human exposure to surface and subsurface soils with contaminant

  19. Investigating the neural basis of cooperative joint action. An EEG hyperscanning study.

    PubMed

    Astolfi, L; Toppi, J; Vogel, P; Mattia, D; Babiloni, F; Ciaramidaro, A; Siniatchkin, M

    2014-01-01

    The aim of the present study is to investigate the neurophysiological basis of the cognitive functions underlying the execution of joint actions, by means of the recent technique called hyperscanning. Neuroelectrical hyperscanning is based on the simultaneous recording of brain activity from multiple subjects and includes the analysis of the functional relation between the brain activity of all the interacting individuals. We recorded simultaneous high density electroencephalography (hdEEG) from 16 pairs of subjects involved in a computerized joint action paradigm, with controlled levels of cooperation. Results of cortical connectivity analysis returned significant differences, in terms of inter-brain functional causal links, between the condition of cooperative joint action and a condition in which the subjects were told they were interacting with a PC, while actually interacting with another human subject. Such differences, described by selected brain connectivity indices, point toward an integration between the two subjects' brain activity in the cooperative condition, with respect to control conditions.

  20. Corrective Action Investigation Plan for Corrective Action Unit 554: Area 23 Release Site, Nevada Test Site, Nevada, Rev. 0 with ROTC No. 1 and ROTC No. 2

    SciTech Connect

    Robert F. Boehlecke

    2004-10-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information for conducting site investigation activities at Corrective Action Unit (CAU) 554: Area 23 Release Site, Nevada Test Site, Nevada. Information presented in this CAIP includes facility descriptions, environmental sample collection objectives, and criteria for the selection and evaluation of environmental samples. Corrective Action Unit 554 is located in Area 23 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 554 is comprised of one Corrective Action Site (CAS), which is: 23-02-08, USTs 23-115-1, 2, 3/Spill 530-90-002. This site consists of soil contamination resulting from a fuel release from underground storage tanks (USTs). Corrective Action Site 23-02-08 is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation prior to evaluating corrective action alternatives and selecting the appropriate corrective action for this CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document for CAU 554. Corrective Action Site 23-02-08 will be investigated based on the data quality objectives (DQOs) developed on July 15, 2004, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; and contractor personnel. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 554. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to CAS 23-02-08. The scope of the corrective action investigation

  1. Investigations to the Antibacterial Mechanism of Action of Kendomycin

    PubMed Central

    A. Elnakady, Yasser; Chatterjee, Indranil; Bischoff, Markus; Rohde, Manfred; Josten, Michaele; Sahl, Hans-Georg; Herrmann, Mathias; Müller, Rolf

    2016-01-01

    Purpose The emergence of bacteria that are resistant to many currently used drugs emphasizes the need to discover and develop new antibiotics that are effective against such multi-resistant strains. Kendomycin is a novel polyketide that has a unique quinone methide ansa structure and various biological properties. This compound exhibits strong antibacterial activity against Gram-negative and Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). Despite the promise of kendomycinin in several therapeutic areas, its mode of action has yet to be identified. Methods In this study, we used a multidisciplinary approach to gain insight into the antibacterial mechanism of this compound. Results The antibacterial activity of kendomycin appears to be bacteriostatic rather than bactericidal. Kendomycin inhibited the growth of the MRSA strain COL at a low concentration (MIC of 5 μg/mL). Proteomic analysis and gene transcription profiling of kendomycin-treated cells indicated that this compound affected the regulation of numerous proteins and genes involved in central metabolic pathways, such as the tricarboxylic acid (TCA) cycle (SdhA) and gluconeogenesis (PckA and GapB), cell wall biosynthesis and cell division (FtsA, FtsZ, and MurAA), capsule production (Cap5A and Cap5C), bacterial programmed cell death (LrgA and CidA), the cellular stress response (ClpB, ClpC, ClpP, GroEL, DnaK, and GrpE), and oxidative stress (AhpC and KatA). Electron microscopy revealed that kendomycin strongly affected septum formation during cell division. Most kendomycin-treated cells displayed incomplete septa with abnormal morphology. Conclusions Kendomycin might directly or indirectly affect the cell division machinery, protein stability, and programmed cell death in S. aureus. Additional studies are still needed to obtain deeper insight into the mode of action of kendomycin. PMID:26795276

  2. Corrective action investigation plan for CAU No. 424: Area 3 Landfill Complex, Tonopah Test Range, Nevada

    SciTech Connect

    1997-04-01

    This Correction Action Investigation Plan contains the environmental sample collection objectives and the criteria for conducting site investigation activities at the Area 3 Landfill Complex, CAU No. 424, which is located at the Tonopah Test Range (TTR). The TTR, included in the Nellis Air Force Range, is approximately 255 kilometers (140 miles) northwest of Las Vegas, nevada. The CAU 424 is comprised of eight individual landfill sites that are located around and within the perimeter of the Area 3 Compound. Due to the unregulated disposal activities commonly associated with early landfill operations, an investigation will be conducted at each CAS to complete the following tasks: identify the presence and nature of possible contaminant migration from the landfills; determine the vertical and lateral extent of possible contaminant migration; ascertain the potential impact to human health and the environment; and provide sufficient information and data to develop and evaluate appropriate corrective action strategies for each CAS.

  3. Corrective Action Investigation plan for Corrective Action Unit 546: Injection Well and Surface Releases, Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Alfred Wickline

    2008-03-01

    Corrective Action Unit (CAU) 546 is located in Areas 6 and 9 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 546 is comprised of two Corrective Action Sites (CASs) listed below: •06-23-02, U-6a/Russet Testing Area •09-20-01, Injection Well These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on November 8, 2007, by representatives of the Nevada Division of Environmental Protection and U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process has been used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 546.

  4. Corrective Action Investigation Plan for Corrective Action Unit 560: Septic Systems, Nevada Test Site, Nevada with ROTC1, Revision 0

    SciTech Connect

    Grant Evenson

    2008-05-01

    Corrective Action Unit (CAU) 560 is located in Areas 3 and 6 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 560 is comprised of the seven corrective action sites (CASs) listed below: • 03-51-01, Leach Pit • 06-04-02, Septic Tank • 06-05-03, Leach Pit • 06-05-04, Leach Bed • 06-59-03, Building CP-400 Septic System • 06-59-04, Office Trailer Complex Sewage Pond • 06-59-05, Control Point Septic System These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on January 22, 2008, by representatives from the Nevada Division of Environmental Protection; U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 560.

  5. Action Research as a Professional Development Activity

    ERIC Educational Resources Information Center

    West, Chad

    2011-01-01

    Reflective teachers are always searching for ways to improve their teaching. When this reflection becomes intentional and systematic, they are engaging in teacher research. This type of research, sometimes called "action research", can help bridge the gap between theory and practice by addressing topics that are relevant to practicing teachers.…

  6. Corrective Action Investigation Plan for Corrective Action Unit 370: T-4 Atmospheric Test Site, Nevada Test Site, Nevada with ROTC-1, Revision 0

    SciTech Connect

    Pat Matthews

    2008-04-01

    Corrective Action Unit (CAU) 370 is located in Area 4 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 370 is comprised of Corrective Action Site (CAS) 04-23-01, Atmospheric Test Site T-4. This site is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and/or implement a corrective action. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for this CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The investigation results may also be used to evaluate improvements in the Soils Project strategy to be implemented. The site will be investigated based on the data quality objectives (DQOs) developed on December 10, 2007, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office; Desert Research Institute; Stoller-Navarro Joint Venture; and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 370. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to the CAS. The scope of the CAI for CAU 370 includes the following activities: • Move surface debris and/or materials, as needed, to facilitate sampling. • Conduct radiological surveys. • Perform field screening. • Collect and submit environmental samples for laboratory analysis to determine whether contaminants of concern are present. • If contaminants of concern are present, collect samples to define the extent of the

  7. Corrective Action Investigation Plan for Corrective Action Unit 371: Johnnie Boy Crater and Pin Stripe Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2009-02-01

    Corrective Action Unit (CAU) 371 is located in Areas 11 and 18 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 371 is comprised of the two corrective action sites (CASs) listed below: • 11-23-05, Pin Stripe Contamination Area • 18-45-01, U-18j-2 Crater (Johnnie Boy) These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on November 19, 2008, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 371. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the corrective action investigation for CAU 371 includes the following activities: • Move surface debris and/or materials, as needed, to facilitate sampling. • Conduct radiological surveys. • Measure in situ external dose rates using thermoluminescent dosimeters or other dose measurement devices. • Collect and submit environmental samples for laboratory analysis to determine internal dose rates. • Combine internal and external dose rates to determine whether total

  8. Corrective Action Investigation Plan for Corrective Action Unit 232: Area 25 Sewage Lagoons, Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    USDOE /NV

    1999-05-01

    The Corrective Action Investigation Plan for Corrective Action Unit 232, Area 25 Sewage Lagoons, has been developed in accordance with the Federal Facility Agreement and Consent Order that was agreed to by the U.S. Department of Energy, Nevada Operations Office; the State of Nevada Division of Environmental Protection; and the U. S. Department of Defense. Corrective Action Unit 232 consists of Corrective Action Site 25-03-01, Sewage Lagoon. Corrective Action Unit 232, Area 25 Sewage Lagoons, received sanitary effluent from four buildings within the Test Cell ''C'' Facility from the mid-1960s through approximately 1996. The Test Cell ''C'' Facility was used to develop nuclear propulsion technology by conducting nuclear test reactor studies. Based on the site history collected to support the Data Quality Objectives process, contaminants of potential concern include volatile organic compounds, semivolatile organic compounds, Resource Conservation and Recovery Act metals, petroleum hydrocarbons, polychlorinated biphenyls, pesticides, herbicides, gamma emitting radionuclides, isotopic plutonium, isotopic uranium, and strontium-90. A detailed conceptual site model is presented in Section 3.0 and Appendix A of this Corrective Action Investigation Plan. The conceptual model serves as the basis for the sampling strategy. Under the Federal Facility Agreement and Consent Order, the Corrective Action Investigation Plan will be submitted to the Nevada Division of Environmental Protection for approval. Field work will be conducted following approval of the plan. The results of the field investigation will support a defensible evaluation of corrective action alternatives in the Corrective Action Decision Document.

  9. Corrective Action Investigation Plan for Corrective Action Unit 552: Area 12 Muckpile and Ponds, Nevada Test Site, Nevada, Rev. 1

    SciTech Connect

    Robert F. Boehlecke

    2005-01-01

    II samples, since the analytical results of those samples will show how far the contamination has travelled in the time period since activities at the site ended. Most of the data necessary to resolve the decisions will be generated from the analysis of environmental samples collected during the CAI for CAU 552. The general purpose of the investigation is to: (1) Identify the presence and nature of COCs. (2) Determine the vertical and lateral extent of identified COCs. (3) Ensure sufficient data is collected to support the selection of a corrective action compliant with all NDEP, ''Resource Conservation and Recovery Act (RCRA), Toxic Substance Control Act (TSCA)'', and DOE requirements. In addition, data will be obtained to support (IDW) disposal and potential future waste management decisions.

  10. Can persuasive messages encourage individuals to create action plans for physical activity?

    PubMed

    Sweet, Shane N; Brawley, Lawrence R; Hatchell, Alexandra; Gainforth, Heather L; Latimer-Cheung, Amy E

    2014-08-01

    Given the positive influence of action planning on physical activity, persuasive messages could be designed to promote action planning. The purpose of this paper was to test action planning messages in two studies. Participants were allocated to one of two message groups, reading either a physical activity only or physical activity plus action planning message (Study 1) and either a gain-framed or loss-framed action planning message (Study 2). The percent of individuals who created an action plan and the quality of the plans were evaluated. In Study 1, individuals in the physical activity plus action planning group created as many action plans as the physical activity only group, but their plans were higher quality. In Study 2, Week 2 differences between the gain- and loss-framed message groups were found for action planning. To our knowledge, these studies were the first to investigate message-induced action planning as a behavior. More research is needed to optimize these messages.

  11. Spontaneous Action and Transformative Learning: Empirical Investigations and Pragmatist Reflections

    ERIC Educational Resources Information Center

    Nohl, Arnd-Michael

    2009-01-01

    Whereas present theories of transformative learning tend to focus on the rational and reflective actor, in this article it is suggested that spontaneous action may play a decisive role in transformative learning too. In the spontaneity of action, novelty finds its way into life, gains momentum, is respected by others and reflected by the actor.…

  12. Addendum to the Corrective Action Investigation Plan for Corrective Action Unit 321: Area 22 Weather Station Fuel Storage, Nevada Test Site, Nevada (Rev. 0, November 2000)

    SciTech Connect

    DOE /NV

    2000-11-03

    This addendum to the Corrective Action Investigation Plan (CAIP) contains the U.S. Department of Energy, Nevada Operations Office's approach to determine the extent of contamination existing at Corrective Action Unit (CAU) 321. This addendum was required when the extent of contamination exceeded the estimate in the original Corrective Action Decision Document (CADD). Located in Area 22 on the Nevada Test Site, Corrective Action Unit 321, Weather Station Fuel Storage, consists of Corrective Action Site 22-99-05, Fuel Storage Area, was used to store fuel and other petroleum products necessary for motorized operations at the historic Camp Desert Rock facility. This facility was operational from 1951 to 1958 and dismantled after 1958. Based on site history and earlier investigation activities at CAU 321, the contaminant of potential concern (COPC) was previously identified as total petroleum hydrocarbons (diesel-range organics). The scope of this corrective action investigation for the Fuel Storage Area will include the selection of biased sample locations to determine the vertical and lateral extent of contamination, collection of soil samples using rotary sonic drilling techniques, and the utilization of field-screening methods to accurately determine the extent of COPC contamination. The results of this field investigation will support a defensible evaluation of corrective action alternatives and be included in the revised CADD.

  13. Aircraft accident investigation: the decision-making in initial action scenario.

    PubMed

    Barreto, Marcia M; Ribeiro, Selma L O

    2012-01-01

    In the complex aeronautical environment, the efforts in terms of operational safety involve the adoption of proactive and reactive measures. The process of investigation begins right after the occurrence of the aeronautical accident, through the initial action. Thus, it is in the crisis scenario, that the person responsible for the initial action makes decisions and gathers the necessary information for the subsequent phases of the investigation process. Within this scenario, which is a natural environment, researches have shown the fragility of rational models of decision making. The theoretical perspective of naturalistic decision making constitutes a breakthrough in the understanding of decision problems demanded by real world. The proposal of this study was to verify if the initial action, after the occurrence of an accident, and the decision-making strategies, used by the investigators responsible for this activity, are characteristic of the naturalistic decision making theoretical approach. To attend the proposed objective a descriptive research was undertaken with a sample of professionals that work in this activity. The data collected through individual interviews were analyzed and the results demonstrated that the initial action environment, which includes restricted time, dynamic conditions, the presence of multiple actors, stress and insufficient information is characteristic of the naturalistic decision making. They also demonstrated that, when the investigators make their decisions, they use their experience and the mental simulation, intuition, improvisation, metaphors and analogues cases, as strategies, all of them related to the naturalistic approach of decision making, in order to satisfy the needs of the situation and reach the objectives of the initial action in the accident scenario.

  14. Aircraft accident investigation: the decision-making in initial action scenario.

    PubMed

    Barreto, Marcia M; Ribeiro, Selma L O

    2012-01-01

    In the complex aeronautical environment, the efforts in terms of operational safety involve the adoption of proactive and reactive measures. The process of investigation begins right after the occurrence of the aeronautical accident, through the initial action. Thus, it is in the crisis scenario, that the person responsible for the initial action makes decisions and gathers the necessary information for the subsequent phases of the investigation process. Within this scenario, which is a natural environment, researches have shown the fragility of rational models of decision making. The theoretical perspective of naturalistic decision making constitutes a breakthrough in the understanding of decision problems demanded by real world. The proposal of this study was to verify if the initial action, after the occurrence of an accident, and the decision-making strategies, used by the investigators responsible for this activity, are characteristic of the naturalistic decision making theoretical approach. To attend the proposed objective a descriptive research was undertaken with a sample of professionals that work in this activity. The data collected through individual interviews were analyzed and the results demonstrated that the initial action environment, which includes restricted time, dynamic conditions, the presence of multiple actors, stress and insufficient information is characteristic of the naturalistic decision making. They also demonstrated that, when the investigators make their decisions, they use their experience and the mental simulation, intuition, improvisation, metaphors and analogues cases, as strategies, all of them related to the naturalistic approach of decision making, in order to satisfy the needs of the situation and reach the objectives of the initial action in the accident scenario. PMID:22317482

  15. Health Activities Project (HAP): Heart Fitness and Action Module.

    ERIC Educational Resources Information Center

    Buller, Dave; And Others

    Contained within this Health Activities Project (HAP) learning packet are activities for children in grades 5-8. Design of the activities centers around the idea that students can control their own health and safety. Within the Heart Fitness and Action Module are teacher and student folios describing five activities which involve students in…

  16. Corrective Action Investigation Plan for Corrective Action Unit 374: Area 20 Schooner Unit Crater Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2010-02-01

    Corrective Action Unit 374 is located in Areas 18 and 20 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 374 comprises the five corrective action sites (CASs) listed below: • 18-22-05, Drum • 18-22-06, Drums (20) • 18-22-08, Drum • 18-23-01, Danny Boy Contamination Area • 20-45-03, U-20u Crater (Schooner) These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on October 20, 2009, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 374.

  17. Corrective Action Investigation Plan for Corrective Action Unit 204: Storage Bunkers, Nevada Test Site, Nevada (December 2002, Revision No.: 0), Including Record of Technical Change No. 1

    SciTech Connect

    NNSA /NSO

    2002-12-12

    The Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 204 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 204 is located on the Nevada Test Site approximately 65 miles northwest of Las Vegas, Nevada. This CAU is comprised of six Corrective Action Sites (CASs) which include: 01-34-01, Underground Instrument House Bunker; 02-34-01, Instrument Bunker; 03-34-01, Underground Bunker; 05-18-02, Chemical Explosives Storage; 05-33-01, Kay Blockhouse; 05-99-02, Explosive Storage Bunker. Based on site history, process knowledge, and previous field efforts, contaminants of potential concern for Corrective Action Unit 204 collectively include radionuclides, beryllium, high explosives, lead, polychlorinated biphenyls, total petroleum hydrocarbons, silver, warfarin, and zinc phosphide. The primary question for the investigation is: ''Are existing data sufficient to evaluate appropriate corrective actions?'' To address this question, resolution of two decision statements is required. Decision I is to ''Define the nature of contamination'' by identifying any contamination above preliminary action levels (PALs); Decision II is to ''Determine the extent of contamination identified above PALs. If PALs are not exceeded, the investigation is completed. If PALs are exceeded, then Decision II must be resolved. In addition, data will be obtained to support waste management decisions. Field activities will include radiological land area surveys, geophysical surveys to identify any subsurface metallic and nonmetallic debris, field screening for applicable contaminants of potential concern, collection and analysis of surface and subsurface soil samples from biased locations, and step-out sampling to define the extent of

  18. Familiarity modulates motor activation while other species' actions are observed: a magnetic stimulation study.

    PubMed

    Amoruso, Lucia; Urgesi, Cosimo

    2016-03-01

    Observing other people's actions facilitates the observer's motor system as compared with observing the same individuals at rest. This motor activation is thought to result from mirror-like activity in fronto-parietal areas, which enhances the excitability of the primary motor cortex via cortico-cortical pathways. Although covert motor activation in response to observed actions has been widely investigated between conspecifics, how humans cope with other species' actions has received less attention. For example, it remains unclear whether the human motor system is activated by observing other species' actions, and whether prior familiarity with the non-conspecific agent modulates this activation. Here, we combined single-pulse transcranial magnetic stimulation and motor-evoked potential recording to explore the impact of familiarity on motor activation during the observation of non-conspecific actions. Videos displaying actions performed either by a conspecific (human) or by a non-conspecific (dog) were shown to individuals who had prior familiarity or no familiarity at all with the non-conspecific agent. We found that, whereas individuals with long-lasting familiarity showed similar levels of motor activation for human and canine actions, individuals who had no familiarity showed higher motor activation for human than for canine actions. These findings suggest that the human motor system is flexible enough to resonate with other species, and that familiarity plays a key role in tuning this ability. PMID:26666833

  19. Investigating social gaze as an action-perception online performance

    PubMed Central

    Grynszpan, Ouriel; Simonin, Jérôme; Martin, Jean-Claude; Nadel, Jacqueline

    2012-01-01

    Gaze represents a major non-verbal communication channel in social interactions. In this respect, when facing another person, one's gaze should not be examined as a purely perceptive process but also as an action-perception online performance. However, little is known about processes involved in the real-time self-regulation of social gaze. The present study investigates the impact of a gaze-contingent viewing window on fixation patterns and the awareness of being the agent moving the window. In face-to-face scenarios played by a virtual human character, the task for the 18 adult participants was to interpret an equivocal sentence which could be disambiguated by examining the emotional expressions of the character speaking. The virtual character was embedded in naturalistic backgrounds to enhance realism. Eye-tracking data showed that the viewing window induced changes in gaze behavior, notably longer visual fixations. Notwithstanding, only half of the participants ascribed the window displacements to their eye movements. These participants also spent more time looking at the eyes and mouth regions of the virtual human character. The outcomes of the study highlight the dissociation between non-volitional gaze adaptation and the self-ascription of agency. Such dissociation provides support for a two-step account of the sense of agency composed of pre-noetic monitoring mechanisms and reflexive processes, linked by bottom-up and top-down processes. We comment upon these results, which illustrate the relevance of our method for studying online social cognition, in particular concerning autism spectrum disorders (ASD) where the poor pragmatic understanding of oral speech is considered linked to visual peculiarities that impede facial exploration. PMID:22529796

  20. Corrective Action Investigation Plan for Corrective Action Unit 552: Area 12 Muckpile and Ponds, Nevada Test Site, Nevada, Rev. No.: 1 with ROTC 1 and 2

    SciTech Connect

    David A. Strand

    2005-01-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 552: Area 12 Muckpile and Ponds, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense. The NTS is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Corrective Action Unit 552 is comprised of the one Corrective Action Site which is 12-23-05, Ponds. One additional CAS, 12-06-04, Muckpile (G-Tunnel Muckpile), was removed from this CAU when it was determined that the muckpile is an active site. A modification to the FFACO to remove CAS 12-06-04 was approved by the Nevada Division of Environmental Protection (NDEP) on December 16, 2004. The G-Tunnel ponds were first identified in the 1991 Reynolds Electrical & Engineering Co., Inc. document entitled, ''Nevada Test Site Inventory of Inactive and Abandoned Facilities and Waste Sites'' (REECo, 1991). Corrective Action Unit 552 is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Therefore, additional information will be obtained by conducting a corrective action investigation (CAI) prior to evaluating and selecting the corrective action alternatives for the site. The CAI will include field inspections, radiological surveys, and sampling of appropriate media. Data will also be obtained to support investigation-derived waste (IDW) disposal and potential future waste management decisions.

  1. Modulation of Brain Activity during Action Observation: Influence of Perspective, Transitivity and Meaningfulness

    PubMed Central

    Hétu, Sébastien; Mercier, Catherine; Eugène, Fanny; Michon, Pierre-Emmanuel; Jackson, Philip L.

    2011-01-01

    The coupling process between observed and performed actions is thought to be performed by a fronto-parietal perception-action system including regions of the inferior frontal gyrus and the inferior parietal lobule. When investigating the influence of the movements' characteristics on this process, most research on action observation has focused on only one particular variable even though the type of movements we observe can vary on several levels. By manipulating the visual perspective, transitivity and meaningfulness of observed movements in a functional magnetic resonance imaging study we aimed at investigating how the type of movements and the visual perspective can modulate brain activity during action observation in healthy individuals. Importantly, we used an active observation task where participants had to subsequently execute or imagine the observed movements. Our results show that the fronto-parietal regions of the perception action system were mostly recruited during the observation of meaningless actions while visual perspective had little influence on the activity within the perception-action system. Simultaneous investigation of several sources of modulation during active action observation is probably an approach that could lead to a greater ecological comprehension of this important sensorimotor process. PMID:21931832

  2. Corrective Action Investigation Plan for Corrective Action Unit 365: Baneberry Contamination Area, Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2010-12-01

    Corrective Action Unit 365 comprises one corrective action site (CAS), CAS 08-23-02, U-8d Contamination Area. This site is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for the CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The site will be investigated based on the data quality objectives (DQOs) developed on July 6, 2010, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for the Baneberry site. The primary release associated with Corrective Action Unit 365 was radiological contamination from the Baneberry nuclear test. Baneberry was an underground weapons-related test that vented significant quantities of radioactive gases from a fissure located in close proximity to ground zero. A crater formed shortly after detonation, which stemmed part of the flow from the fissure. The scope of this investigation includes surface and shallow subsurface (less than 15 feet below ground surface) soils. Radionuclides from the Baneberry test with the potential to impact groundwater are included within the Underground Test Area Subproject. Investigations and corrective actions associated with the Underground Test Area Subproject include the radiological inventory resulting from the Baneberry test.

  3. Corrective Action Investigation Plan for Corrective Action Unit 139: Waste Disposal Sites, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    Grant Evenson

    2006-04-01

    Corrective Action Unit (CAU) 139 is located in Areas 3, 4, 6, and 9 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 139 is comprised of the seven corrective action sites (CASs) listed below: (1) 03-35-01, Burn Pit; (2) 04-08-02, Waste Disposal Site; (3) 04-99-01, Contaminated Surface Debris; (4) 06-19-02, Waste Disposal Site/Burn Pit; (5) 06-19-03, Waste Disposal Trenches; (6) 09-23-01, Area 9 Gravel Gertie; and (7) 09-34-01, Underground Detection Station. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives with the exception of CASs 09-23-01 and 09-34-01. Regarding these two CASs, CAS 09-23-01 is a gravel gertie where a zero-yield test was conducted with all contamination confined to below ground within the area of the structure, and CAS 09-34-01 is an underground detection station where no contaminants are present. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for the other five CASs where information is insufficient. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on January 4, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 139.

  4. Corrective Action Investigation Plan for Corrective Action Unit 573: Alpha Contaminated Sites, Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Matthews, Patrick

    2014-05-01

    Corrective Action Unit (CAU) 573 is located in Area 5 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. CAU 573 is a grouping of sites where there has been a suspected release of contamination associated with non-nuclear experiments and nuclear testing. This document describes the planned investigation of CAU 573, which comprises the following corrective action sites (CASs): • 05-23-02, GMX Alpha Contaminated Area • 05-45-01, Atmospheric Test Site - Hamilton These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives.

  5. Corrective Action Investigation Plan for Corrective Action Unit 166: Storage Yards and Contaminated Materials, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    David Strand

    2006-06-01

    Corrective Action Unit 166 is located in Areas 2, 3, 5, and 18 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit (CAU) 166 is comprised of the seven Corrective Action Sites (CASs) listed below: (1) 02-42-01, Cond. Release Storage Yd - North; (2) 02-42-02, Cond. Release Storage Yd - South; (3) 02-99-10, D-38 Storage Area; (4) 03-42-01, Conditional Release Storage Yard; (5) 05-19-02, Contaminated Soil and Drum; (6) 18-01-01, Aboveground Storage Tank; and (7) 18-99-03, Wax Piles/Oil Stain. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on February 28, 2006, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and Bechtel Nevada. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 166. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the CAI for CAU 166 includes the following activities: (1) Move surface debris and/or materials, as needed, to facilitate sampling. (2) Conduct radiological surveys. (3) Perform field screening. (4) Collect and submit environmental samples for laboratory analysis to determine if

  6. Corrective Action Investigation Plan for Corrective Action Unit 106: Areas 5, 11 Frenchman Flat Atmospheric Sites, Nevada National Security Site, Nevada

    SciTech Connect

    Patrick Matthews

    2011-07-01

    Corrective Action Unit 106 comprises the four corrective action sites (CASs) listed below: • 05-20-02, Evaporation Pond • 05-23-05, Atmospheric Test Site - Able • 05-45-04, 306 GZ Rad Contaminated Area • 05-45-05, 307 GZ Rad Contaminated Area These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on January 19, 2010, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 106. The presence and nature of contamination at CAU 106 will be evaluated based on information collected from a field investigation. The CAU includes land areas impacted by the release of radionuclides from groundwater pumping during the Radionuclide Migration study program (CAS 05-20-02), a weapons-related airdrop test (CAS 05-23-05), and unknown support activities at two sites (CAS 05-45-04 and CAS 05-45-05). The presence and nature of contamination from surface-deposited radiological contamination from CAS 05-23-05, Atmospheric Test Site - Able, and other types of releases (such as migration and excavation as well as any potential releases discovered during the investigation) from the remaining three CASs will be evaluated using soil samples collected from the locations

  7. Corrective action investigation plan: Area 2 Photo Skid 16 Wastewater Pit, Corrective Action Unit 332. Revision 1

    SciTech Connect

    1997-01-01

    This Corrective Action Investigation Plan (CAIP) contains a detailed description and plan for an environmental investigation of the Area 2 Photo Skid 16 Wastewater Pit. The site is located in Area 2 of the Nevada Test Site. The Photo Skid Wastewater Pit was used for disposal of photochemical process waste, and there is a concern that such disposal may have released photochemicals and metals to the soil beneath the pit and adjacent to it. The purpose of this investigation is to identify the presence and nature of contamination present in and adjacent to the wastewater pit and to determine the appropriate course of environmental response action for the site. The potential courses of action for the site are clean closure through remediation, closure in place (with or without remediation), or no further action.

  8. Corrective Action Investigation Plan for Corrective Action Unit 98: Frenchman Flat, Nevada Test Site, Nevada (Revision 1)

    SciTech Connect

    USDOE /NV

    1999-07-01

    This Corrective Action Investigation Plan (CAIP) has been developed for Frenchman Flat Corrective Action Unit (CAU) 98. The Frenchman Flat CAU is located along the eastern border of the Nevada Test Site (NTS) and includes portions of Areas 5 and 11. The Frenchman Flat CAU constitutes one of several areas of the Nevada Test Site used for underground nuclear testing in the past. The nuclear tests resulted in groundwater contamination in the vicinity as well as downgradient of the underground test areas. The CAIP describes the Corrective Action Investigation (CAI) to be conducted at the Frenchman Flat CAU to evaluate the extent of contamination in groundwater due to the underground nuclear testing. The Frenchman Flat CAI will be conducted by the Underground Test Area (UGTA) Project which is a part of the U.S. Department of Energy, Nevada Operations Office (DOE/NV) Environmental Restoration Project. The CAIP is a requirement of the Federal Facility Agreement and Consent Order (FFACO) (1996 ) agreed to by the U.S. Department of Energy (DOE), the Nevada Division of Environmental Protection (NDEP), and the U.S. Department of Defense (DoD). Based on the general definition of a CAI from Section IV.14 of the FFACO, the purpose of the CAI is ''...to gather data sufficient to characterize the nature, extent, and rate of migration or potential rate of migration from releases or discharges of pollutants or contaminants and/or potential releases or discharges from corrective action units identified at the facilities...'' (FFACO, 1996). However, for the Underground Test Area (UGTA) CAUs, ''...the objective of the CAI process is to define boundaries around each UGTA CAU that establish areas that contain water that may be unsafe for domestic and municipal use.'', as stated in Appendix VI of the FFACO (1996). According to the UGTA strategy (Appendix VI of the FFACO), the CAI of a given CAU starts with the evaluation of the existing data. New data collection activities are generally

  9. The Biodiversity Community Action Project: An STS Investigation

    ERIC Educational Resources Information Center

    Aidin, Amirshokoohi; Mahsa, Kazempour

    2010-01-01

    The Biodiversity Community Action Project is a stimulating and vigorous project that allows students to gain an in-depth understanding of the interconnection between organisms and their environments as well as the connection of science to their lives and society. It addresses key content standards in the National Science Education Standards and…

  10. Investigating the Benefits of Participatory Action Research for Environmental Education

    ERIC Educational Resources Information Center

    Bywater, Krista

    2014-01-01

    Environmental education (EE) continues to focus on enhancing people's ecological knowledge to encourage sustainable actions. This deficit approach presumes that once informed about environmental harms, people will work towards sustainable solutions for healthy societies. Yet research overwhelmingly demonstrates that knowledge of environmental…

  11. Differential activation of the lateral premotor cortex during action observation

    PubMed Central

    2010-01-01

    Background Action observation leads to neural activation of the human premotor cortex. This study examined how the level of motor expertise (expert vs. novice) in ballroom dancing and the visual viewpoint (internal vs. external viewpoint) influence this activation within different parts of this area of the brain. Results Sixteen dance experts and 16 novices observed ballroom dance videos from internal or external viewpoints while lying in a functional magnetic resonance imaging scanner. A conjunction analysis of all observation conditions showed that action observation activated distinct networks of premotor, parietal, and cerebellar structures. Experts revealed increased activation in the ventral premotor cortex compared to novices. An internal viewpoint led to higher activation of the dorsal premotor cortex. Conclusions The present results suggest that the ventral and dorsal premotor cortex adopt differential roles during action observation depending on the level of motor expertise and the viewpoint. PMID:20673366

  12. Corrective Action Investigation Plan for Corrective Action Unit No. 423: Building 03-60 Underground Discharge Point, Tonopah Test Range, Nevada

    SciTech Connect

    DOE /NV

    1997-10-01

    This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV), the State of Nevada Division of Environmental Protection (NDEP), and the US Department of Defense. The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUS) or Corrective Action Sites (CASs) (FFACO, 1996). As per the FFACO (1996), CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites. Corrective Action Units consist of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at CAU No. 423, the Building 03-60 Underground Discharge Point (UDP), which is located in Area 3 at the Tonopah Test Range (TTR). The TTR, part of the Nellis Air Force Range, is approximately 225 kilometers (km) (140 miles [mi]) northwest of Las Vegas, Nevada (Figures 1-1 and 1-2). Corrective Action Unit No. 423 is comprised of only one CAS (No. 03-02-002-0308), which includes the Building 03-60 UDP and an associated discharge line extending from Building 03-60 to a point approximately 73 meters (m) (240 feet [ft]) northwest as shown on Figure 1-3.

  13. Both novelty and expertise increase action observation network activity.

    PubMed

    Liew, Sook-Lei; Sheng, Tong; Margetis, John L; Aziz-Zadeh, Lisa

    2013-01-01

    Our experiences with others affect how we perceive their actions. In particular, activity in bilateral premotor and parietal cortices during action observation, collectively known as the action observation network (AON), is modulated by one's expertise with the observed actions or individuals. However, conflicting reports suggest that AON activity is greatest both for familiar and unfamiliar actions. The current study examines the effects of different types and amounts of experience (e.g., visual, interpersonal, personal) on AON activation. fMRI was used to scan 16 healthy participants without prior experience with individuals with amputations (novices), 11 experienced occupational therapists (OTs) who had varying amounts of experience with individuals with amputations, and one individual born with below-elbow residual limbs (participant CJ), as they viewed video clips of goal-matched actions performed by an individual with residual limbs and by an individual with hands. Participants were given increased visual exposure to actions performed by both effectors midway through the scanning procedure. Novices demonstrated a large AON response to the initial viewing of an individual with residual limbs compared to one with hands, but this signal was attenuated after they received visual exposure to both effectors. In contrast, OTs, who had moderate familiarity with residual limbs, demonstrated a lower AON response upon initial viewing-similar to novices after they received visual exposure. At the other extreme, CJ, who has extreme familiarity with residual limbs both visually and motorically, shows a largely increased left-lateralized AON response, exceeding that of novices and experienced OTs, when viewing the residual limb compared to hand actions. These results suggest that a nuanced model of AON engagement is needed to explain how cases of both extreme experience (CJ) and extreme novelty (novices) can result in the greatest AON activity.

  14. Corrective Action Investigation Plan for Corrective Action Unit 561: Waste Disposal Areas, Nevada Test Site, Nevada with ROTC 1, Revision 0

    SciTech Connect

    Grant Evenson

    2008-07-01

    Corrective Action Unit (CAU) 561 is located in Areas 1, 2, 3, 5, 12, 22, 23, and 25 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 561 is comprised of the 10 corrective action sites (CASs) listed below: • 01-19-01, Waste Dump • 02-08-02, Waste Dump and Burn Area • 03-19-02, Debris Pile • 05-62-01, Radioactive Gravel Pile • 12-23-09, Radioactive Waste Dump • 22-19-06, Buried Waste Disposal Site • 23-21-04, Waste Disposal Trenches • 25-08-02, Waste Dump • 25-23-21, Radioactive Waste Dump • 25-25-19, Hydrocarbon Stains and Trench These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 28, 2008, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 561. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS. The scope of the Corrective Action Investigation for CAU 561 includes the following activities: • Move surface debris and/or materials, as needed, to facilitate sampling. • Conduct radiological surveys

  15. Measurement of action spectra of light-activated processes

    NASA Astrophysics Data System (ADS)

    Ross, Justin; Zvyagin, Andrei V.; Heckenberg, Norman R.; Upcroft, Jacqui; Upcroft, Peter; Rubinsztein-Dunlop, Halina H.

    2006-01-01

    We report on a new experimental technique suitable for measurement of light-activated processes, such as fluorophore transport. The usefulness of this technique is derived from its capacity to decouple the imaging and activation processes, allowing fluorescent imaging of fluorophore transport at a convenient activation wavelength. We demonstrate the efficiency of this new technique in determination of the action spectrum of the light mediated transport of rhodamine 123 into the parasitic protozoan Giardia duodenalis.

  16. Corrective action investigation plan for Corrective Action Unit Number 423: Building 03-60 Underground Discharge Point, Tonopah Test Range, Nevada

    SciTech Connect

    1997-10-27

    This Corrective Action Investigation Plan (CAIP) contains the environmental sample collection objectives and the criteria for conducting site investigation activities at Corrective Action Unit (CAU) Number 423, the Building 03-60 Underground Discharge Point (UDP), which is located in Area 3 at the Tonopah Test Range (TTR). The TTR, part of the Nellis Air Force Range, is approximately 225 kilometers (140 miles) northwest of Las Vegas, Nevada. CAU Number 423 is comprised of only one Corrective Action Site (CAS) which includes the Building 03-60 UDP and an associated discharge line extending from Building 03-60 to a point approximately 73 meters (240 feet) northwest. The UDP was used between approximately 1965 and 1990 to dispose of waste fluids from the Building 03-60 automotive maintenance shop. It is likely that soils surrounding the UDP have been impacted by oil, grease, cleaning supplies and solvents as well as waste motor oil and other automotive fluids released from the UDP.

  17. Corrective Action Investigation Plan for Corrective Action Unit 571: Area 9 Yucca Flat Plutonium Dispersion Sites, Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Bailey, Bernadine; Matthews, Patrick

    2013-07-01

    CAU 571 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 571, which comprises the following corrective action sites (CASs): • 09-23-03, Atmospheric Test Site S-9F • 09-23-04, Atmospheric Test Site T9-C • 09-23-12, Atmospheric Test Site S-9E • 09-23-13, Atmospheric Test Site T-9D • 09-45-01, Windrows Crater These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the investigation report. The sites will be investigated based on the data quality objectives (DQOs) developed on March 6, 2013, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (now the Nevada Field Office). The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 571. The site investigation process will also be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The potential contamination sources associated with CAU 571 CASs are from nuclear testing activities. The DQO process resulted in an assumption that total effective dose (TED) within a default contamination boundary exceeds the final action level (FAL) and requires corrective action. The presence and nature of contamination outside the default

  18. Corrective Action Investigation Plan for Corrective Action Unit 541: Small Boy Nevada National Security Site and Nevada Test and Training Range, Nevada with ROTC 1

    SciTech Connect

    Matthews, Patrick

    2014-09-01

    Corrective Action Unit (CAU) 541 is co-located on the boundary of Area 5 of the Nevada National Security Site and Range 65C of the Nevada Test and Training Range, approximately 65 miles northwest of Las Vegas, Nevada. CAU 541 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 541, which comprises the following corrective action sites (CASs): 05-23-04, Atmospheric Tests (6) - BFa Site; 05-45-03, Atmospheric Test Site - Small Boy. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the investigation report. The sites will be investigated based on the data quality objectives (DQOs) developed on April 1, 2014, by representatives of the Nevada Division of Environmental Protection; U.S. Air Force; and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Field Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 541. The site investigation process also will be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The potential contamination sources associated with CASs 05-23-04 and 05-45-03 are from nuclear testing activities conducted at the Atmospheric Tests (6) - BFa Site and Atmospheric Test Site - Small Boy sites. The presence and nature of

  19. Corrective Action Investigation Plan for Corrective Action Unit 224: Decon Pad and Septic Systems Nevada Test Site, Nevada, Rev. No.: 0, with ROTC 1 and 2

    SciTech Connect

    David A. Strand

    2004-04-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 224: Decon Pad and Septic Systems, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense (DoD). The NTS is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Corrective Action Unit 224 is comprised of the nine Corrective Action Sites (CASs) listed below: 02-04-01, Septic Tank (Buried); 03-05-01, Leachfield; 05-04-01, Septic Tanks (4)/Discharge Area; 06-03-01, Sewage Lagoons (3); 06-05-01, Leachfield; 06-17-04, Decon Pad and Wastewater Catch; 06-23-01, Decon Pad Discharge Piping; 11-04-01, Sewage Lagoon; and 23-05-02, Leachfield. Corrective Action Sites 06-05-01, 06-23-01, and 23-05-02 were identified in the 1991 Reynolds Electrical & Engineering Co., Inc. (REECo) inventory (1991). The remaining sites were identified during review of various historical documents. Additional information will be obtained by conducting a corrective action investigation (CAI) prior to evaluating and selecting a corrective action alternative for each CAS. The CAI will include field inspections, radiological and geological surveys, and sample collection. Data will also be obtained to support investigation-derived waste (IDW) disposal and potential future waste management decisions.

  20. The Internal Anticipation of Sensory Action Effects: When Action Induces FFA and PPA Activity.

    PubMed

    Kühn, Simone; Seurinck, Ruth; Fias, Wim; Waszak, Florian

    2010-01-01

    Voluntary action - in particular the ability to produce desired effects in the environment - is fundamental to human existence. According to ideomotor theory we can achieve goals in the environment by means of anticipating their outcomes. We aimed at providing neurophysiological evidence for the assumption that performing actions calls for the activation of brain areas associated with the sensory effects usually evoked by the actions. We conducted an fMRI study in which right and left button presses lead to the presentation of face and house pictures. We compared a baseline phase with the same phase after participants experienced the association between button presses and pictures. We found an increase in the parahippocampal place area (PPA) for the response that has been associated with house pictures and fusiform face area (FFA) for the response that has been coupled with face pictures. This observation constitutes support for ideomotor theory.

  1. Corrective Action Investigation Plan for Corrective Action Unit 335: Area 6 Injection Well and Drain Pit, Nevada Test Site, Nevada

    SciTech Connect

    DOE /NV

    2000-12-01

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 335, Area 6 Injection Well and Drain Pit, under the Federal Facility Agreement and Consent Order. Corrective Action Unit 335 consists of three Corrective Action Sites (CASs). The CAU is located in the Well 3 Yard in Area 6 at the Nevada Test Site. Historical records indicate that the Drain Pit (CAS 06-23-03) received effluent from truck-washing; the Drums/Oil Waste/Spill (CAS 06-20-01) consisted of four 55-gallon drums containing material removed from the Cased Hole; and the Cased Hole (CAS 06-20-02) was used for disposal of used motor oil, wastewater, and debris. These drums were transported to the Area 5 Hazardous Waste Accumulation Site in July 1991; therefore, they are no longer on site and further investigation or remediation efforts are not required. Consequently, CAS 06-20-01 will be closed with no further action and details of this decision will be described in the Closure Report for this CAU. Any spills that may have been associated with this CAS will be investigated and addressed under CAS 06-20-02. Field investigation efforts will be focused on the two remaining CASs. The scope of the investigation will center around identifying any contaminants of potential concern (COPCs) and, if present, determining the vertical and lateral extent of contamination. The COPCs for the Drain Pit include: total volatile/ semivolatile organic compounds, total petroleum hydrocarbons (gasoline-and diesel-range organics), ethylene glycol monobutyl ether, polychlorinated biphenyls, total Resource Conservation and Recovery Act metals, and radionuclides. The COPCs for the Cased Hole include: total volatile/ semivolatile organic compounds, total petroleum hydrocarbons (diesel-range organics only), and total Resource Conservation an d

  2. Active Spacecraft Potential Control Investigation

    NASA Astrophysics Data System (ADS)

    Torkar, K.; Nakamura, R.; Tajmar, M.; Scharlemann, C.; Jeszenszky, H.; Laky, G.; Fremuth, G.; Escoubet, C. P.; Svenes, K.

    2016-03-01

    In tenuous plasma the floating potential of sunlit spacecraft reaches tens of volts, positive. The corresponding field disturbs measurements of the ambient plasma by electron and ion sensors and can reduce micro-channel plate lifetime in electron detectors owing to large fluxes of attracted photoelectrons. Also the accuracy of electric field measurements may suffer from a high spacecraft potential. The Active Spacecraft Potential Control (ASPOC) neutralizes the spacecraft potential by releasing positive charge produced by indium ion emitters. The method has been successfully applied on other spacecraft such as Cluster and Double Star. Two ASPOC units are present on each spacecraft. Each unit contains four ion emitters, whereby one emitter per instrument is operated at a time. ASPOC for the Magnetospheric Multiscale (MMS) mission includes new developments in the design of the emitters and the electronics. New features include the use of capillaries instead of needles, new materials for the emitters and their internal thermal insulators, an extended voltage and current range of the electronics, both for ion emission and heating purposes, and a more capable control software. This enables lower spacecraft potentials, higher reliability, and a more uniform potential structure in the spacecraft's sheath compared to previous missions. Results from on-ground testing demonstrate compliance with requirements. Model calculations confirm the findings from previous applications that the plasma measurements will not be affected by the beam's space charge. Finally, the various operating modes to adapt to changing boundary conditions are described along with the main data products.

  3. 19 CFR 206.54 - Investigations with respect to extension of action.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... INVESTIGATIONS INVESTIGATIONS RELATING TO GLOBAL AND BILATERAL SAFEGUARD ACTIONS, MARKET DISRUPTION, TRADE...) Limited disclosure of certain confidential business information under administrative protective order... available to an authorized applicant under administrative protective order all confidential...

  4. Corrective Action Investigation Plan for Corrective Action Unit 543: Liquid Disposal Units Nevada Test Site, Nevada, Rev. No.: 0 with ROTC 1 and 2

    SciTech Connect

    David A. Strand

    2004-05-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information including facility descriptions, environmental sample collection objectives, and criteria for conducting site investigation activities at Corrective Action Unit (CAU) 543: Liquid Disposal Units, Nevada Test Site (NTS), Nevada. This CAIP has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S Department of Defense (DoD). Corrective Action Unit 543 is located in Area 6 and Area 15 of the NTS, which is approximately 65 miles (mi) northwest of Las Vegas, Nevada (Figure 1-1). Seven corrective action sites (CASs) comprise CAU 543 and are listed below: (1) 06-07-01, Decon Pad; (2) 15-01-03, Aboveground Storage Tank; (3) 15-04-01, Septic Tank; (4) 15-05-01, Leachfield; (5) 15-08-01, Liquid Manure Tank; (6) 15-23-01, Underground Radioactive Material Area; and (7) 15-23-03, Contaminated Sump, Piping. Corrective Action Site 06-07-01, Decon Pad, is located in Area 6 and consists of the Area 6 Decontamination Facility and its components that are associated with decontamination of equipment, vehicles, and materials related to nuclear testing. The six CASs in Area 15 are located at the U.S. Environmental Protection Agency (EPA) Farm and are related to waste disposal activities at the EPA Farm. The EPA Farm was a fully-functional dairy associated with animal experiments conducted at the on-site laboratory. The corrective action investigation (CAI) will include field inspections, video-mole surveys, and sampling of media, where appropriate. Data will also be obtained to support waste management decisions. The CASs within CAU 543 are being investigated because hazardous and/or radioactive constituents may be present at concentrations that could potentially pose a threat to human health and the environment. The seven CASs in CAU 543 primarily consist of

  5. Corrective Action Investigation Plan for Corrective Action Unit 252: Area 25 Engine Test Stand 1 Decontamination Pad, Nevada Test Site, Nevada

    SciTech Connect

    U.S. Department of Energy, Nevada Operations Office

    1999-08-20

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit 252 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 252 consists of Corrective Action Site (CAS) 25-07-02, Engine Test Stand-1 (ETS-1) Decontamination Pad. Located in Area 25 at the intersection of Road H and Road K at the Nevada Test Site, ETS-1 was designed for use as a mobile radiation checkpoint and for vehicle decontamination. The CAS consists of a concrete decontamination pad with a drain, a gravel-filled sump, two concrete trailer pads, and utility boxes. Constructed in 1966, the ETS-1 facility was part of the Nuclear Rocket Development Station (NRDS) complex and used to test nuclear rockets. The ETS-1 Decontamination Pad and mobile radiation check point was built in 1968. The NRDS complex ceased primary operations in 1973. Based on site history, the focus of the field investigation activities will be to determine if any primary contaminants of potential concern (COPCs) (including radionuclides, total volatile organic compounds, total semivolatile organic compounds, total petroleum hydrocarbons as diesel-range organics, Resource Conservation and Recovery Act metals, total pesticides, and polychlorinated biphenyls) are present at this site. Vertical extent of migration of suspected vehicle decontamination effluent COPCs is expected to be less than 12 feet below ground surface. Lateral extent of migration of COPCs is expected to be limited to the sump area or near the northeast corner of the decontamination pad. Using a biased sampling approach, near-surface and subsurface sampling will be conducted at the suspected worst-case areas including the sump and soil near the northeast corner of the decontamination pad. The results of this field investigation will support a defensible e valuation

  6. Propagation of Action Potentials: An Active Participation Exercise.

    ERIC Educational Resources Information Center

    Felsten, Gary

    1998-01-01

    Describes an active participation exercise that demonstrates the propagation of action potentials (the ability to transmit information through the neural network, dependent upon chemical interactions in the brain). Students assume the structure and function of the network by lining up around the room and communicating through hand signals and…

  7. Corrective Action Investigation Plan for Corrective Action Unit 410: Waste Disposal Trenches, Tonopah Test Range, Nevada, Revision 0 (includes ROTCs 1, 2, and 3)

    SciTech Connect

    NNSA /NV

    2002-07-16

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 410 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 410 is located on the Tonopah Test Range (TTR), which is included in the Nevada Test and Training Range (formerly the Nellis Air Force Range) approximately 140 miles northwest of Las Vegas, Nevada. This CAU is comprised of five Corrective Action Sites (CASs): TA-19-002-TAB2, Debris Mound; TA-21-003-TANL, Disposal Trench; TA-21-002-TAAL, Disposal Trench; 09-21-001-TA09, Disposal Trenches; 03-19-001, Waste Disposal Site. This CAU is being investigated because contaminants may be present in concentrations that could potentially pose a threat to human health and/or the environment, and waste may have been disposed of with out appropriate controls. Four out of five of these CASs are the result of weapons testing and disposal activities at the TTR, and they are grouped together for site closure based on the similarity of the sites (waste disposal sites and trenches). The fifth CAS, CAS 03-19-001, is a hydrocarbon spill related to activities in the area. This site is grouped with this CAU because of the location (TTR). Based on historical documentation and process know-ledge, vertical and lateral migration routes are possible for all CASs. Migration of contaminants may have occurred through transport by infiltration of precipitation through surface soil which serves as a driving force for downward migration of contaminants. Land-use scenarios limit future use of these CASs to industrial activities. The suspected contaminants of potential concern which have been identified are volatile organic compounds; semivolatile organic compounds; high explosives; radiological constituents including depleted uranium

  8. Corrective Action Investigation Plan for Corrective Action Unit 511: Waste Dumps (Piles & Debris), Nevada Test Site, Nevada, Rev. No.: 0 with ROTC 1

    SciTech Connect

    David A. Strand

    2004-08-01

    This Corrective Action Investigation Plan for Corrective Action Unit 511: Waste Dumps (Piles & Debris), Nevada Test Site, Nevada, has been developed in accordance with the Federal Facility Agreement and Consent Order that was agreed to by the State of Nevada, U.S. Department of Energy, and the U.S. Department of Defense. The general purpose of the investigation is to ensure adequate data are collected to provide sufficient and reliable information to identify, evaluate, and select viable corrective actions. This Corrective Action Investigation Plan provides investigative details for CAU 511, whereas programmatic aspects of this project are discussed in the ''Project Management Plan'' (DOE/NV, 1994). General field and laboratory quality assurance and quality control issues are presented in the ''Industrial Sites Quality Assurance Project Plan'' (NNSA/NV, 2002). Health and safety aspects of the project are documented in the current version of the Environmental Engineering Services Contractor's Health and Safety Plan and will be supplemented with a site-specific safety basis document. Corrective Action Unit 511 is comprised of the following nine corrective action sites in Nevada Test Site Areas 3, 4, 6, 7, 18, and 19: (1) 03-08-02, Waste Dump (Piles & Debris); (2) 03-99-11, Waste Dump (Piles); (3) 03-99-12, Waste Dump (Piles & Debris); (4) 04-99-04, Contaminated Trench/Berm; (5) 06-16-01, Waste Dump (Piles & Debris); (6) 06-17-02, Scattered Ordnance/Automatic Weapons Range; (7) 07-08-01, Contaminated Mound; (8) 18-99-10, Ammunition Dump; and (9) 19-19-03, Waste Dump (Piles & Debris). Corrective Action Sites 18-99-10 and 19-19-03 were identified after a review of the ''1992 RCRA Part B Permit Application for Waste Management Activities at the Nevada Test Site, Volume IV, Section L Potential Solid Waste Management Unit'' (DOE/NV, 1992). The remaining seven sites were first identified in the 1991 Reynolds Electrical & Engineering Co., Inc. document entitled, ''Nevada

  9. Disabling Conditions: Investigating Instructional Leadership Teams in Action

    ERIC Educational Resources Information Center

    Weiner, Jennie Miles

    2014-01-01

    This study investigated why and how principals selected members for their instructional leadership team (ILT) and how this selection criteria and process may have impacted team members' understandings of, and behaviors on, the team. Qualitative methods, specifically interviews and observations, were used to explore team members'…

  10. Investigating the Rights of Youths. Students in Action.

    ERIC Educational Resources Information Center

    Insights on Law & Society, 2002

    2002-01-01

    Focuses on the rights of youth in three articles: (1) "Equal Justice? Girls in the Juvenile Justice System"; (2) "Child Labor: An International Challenge"; and (3) "Should Minors Have Access to Violent Video Games?" Includes learning activities at the end of each article. (CMK)

  11. Corrective Action Investigation Plan for Corrective Action Unit 106: Areas 5, 11 Frenchman Flat Atmospheric Sites, Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2010-04-01

    Corrective Action Unit (CAU) 106 is located in Area 5 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 106 comprises the five corrective action sites (CASs) listed below: •05-23-02, GMX Alpha Contaminated Area •05-23-05, Atmospheric Test Site - Able •05-45-01, Atmospheric Test Site - Hamilton •05-45-04, 306 GZ Rad Contaminated Area •05-45-05, 307 GZ Rad Contaminated Area These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on January 19, 2010, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 106. The presence and nature of contamination at CAU 106 will be evaluated based on information collected from a field investigation. The CAU includes land areas impacted by the release of radionuclides from a weapons-effect tower test (CAS 05-45-01), a weapons-related airdrop test (CAS 05-23-05), “equation of state” experiments (CAS 05-23-02), and unknown support activities at two sites (CAS 05-45-04 and CAS 05-45-05). Surface-deposited radiological contamination will be evaluated based on a comparison of the total effective dose (TED) at sample plot locations to the dose

  12. Corrective Action Investigation Plan for Corrective Action Unit 105: Area 2 Yucca Flat Atmospheric Test Sites Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2012-09-01

    Corrective Action Unit (CAU) 105 is located in Area 2 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. CAU 105 is a geographical grouping of sites where there has been a suspected release of contamination associated with atmospheric nuclear testing. This document describes the planned investigation of CAU 105, which comprises the following corrective action sites (CASs): • 02-23-04, Atmospheric Test Site - Whitney • 02-23-05, Atmospheric Test Site T-2A • 02-23-06, Atmospheric Test Site T-2B • 02-23-08, Atmospheric Test Site T-2 • 02-23-09, Atmospheric Test Site - Turk These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 30, 2012, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 105. The site investigation process will also be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The potential contamination sources associated with all CAU 105 CASs are from atmospheric nuclear testing activities. The presence and nature of contamination at CAU

  13. Corrective Action Investigation Plan for Corrective Action Unit 409: Other Waste Sites, Tonopah Test Range, Nevada (Rev. 0)

    SciTech Connect

    DOE /NV

    2000-10-05

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 409 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 409 consists of three Corrective Action Sites (CASs): TA-53-001-TAB2, Septic Sludge Disposal Pit No.1; TA-53-002-TAB2, Septic Sludge Disposal Pit No.2; and RG-24-001-RGCR, Battery Dump Site. The Septic Sludge Disposal Pits are located near Bunker Two, close to Area 3, on the Tonopah Test Range. The Battery Dump Site is located at the abandoned Cactus Repeater Station on Cactus Peak. The Cactus Repeater Station was a remote, battery-powered, signal repeater station. The two Septic Sludge Disposal Pits were suspected to be used through the late 1980s as disposal sites for sludge from septic tanks located in Area 3. Based on site history collected to support the Data Quality Objectives process, contaminants of potential concern are the same for the disposal pits and include: volatile organic compounds (VOCs), semivolatile organic compounds, total petroleum hydrocarbons (TPHs) as gasoline- and diesel-range organics, polychlorinated biphenyls, Resource Conservation and Recovery Act metals, and radionuclides (including plutonium and depleted uranium). The Battery Dump Site consists of discarded lead-acid batteries and associated construction debris, placing the site in a Housekeeping Category and, consequently, no contaminants are expected to be encountered during the cleanup process. The corrective action the at this CAU will include collection of discarded batteries and construction debris at the Battery Dump Site for proper disposal and recycling, along with photographic documentation as the process progresses. The corrective action for the remaining CASs involves the collection of background radiological data through borings drilled at

  14. Functionally Optimized Neuritogenic Farinosone C Analogs: SAR-Study and Investigations on Their Mode of Action

    PubMed Central

    2013-01-01

    Several natural products derived from entomopathogenic fungi have been shown to initiate neuronal differentiation in the rat pheochromocytoma PC12 cell line. After the successful completion of the total synthesis program, the reduction of structural complexity while retaining biological activity was targeted. In this study, farinosone C served as a lead structure and inspired the preparation of small molecules with reduced complexity, of which several were able to induce neurite outgrowth. This allowed for the elaboration of a detailed structure–activity relationship. Investigations on the mode of action utilizing a computational similarity ensemble approach suggested the involvement of the endocannabinoid system as potential target for our analogs and also led to the discovery of four potent new endocannabinoid transport inhibitors. PMID:24900793

  15. Corrective Action Investigation Plan for Corrective Action Unit 570: Area 9 Yucca Flat Atmospheric Test Sites Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2012-08-01

    CAU 570 comprises the following six corrective action sites (CASs): • 02-23-07, Atmospheric Test Site - Tesla • 09-23-10, Atmospheric Test Site T-9 • 09-23-11, Atmospheric Test Site S-9G • 09-23-14, Atmospheric Test Site - Rushmore • 09-23-15, Eagle Contamination Area • 09-99-01, Atmospheric Test Site B-9A These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 30, 2012, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 570. The site investigation process will also be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The presence and nature of contamination at CAU 570 will be evaluated based on information collected from a field investigation. Radiological contamination will be evaluated based on a comparison of the total effective dose at sample locations to the dose-based final action level. The total effective dose will be calculated as the total of separate estimates of internal and external dose. Results from the analysis of soil samples will be used to calculate internal radiological

  16. Expanding the mirror: vicarious activity for actions, emotions, and sensations.

    PubMed

    Keysers, Christian; Gazzola, Valeria

    2009-12-01

    We often empathically share the states of others. The discovery of 'mirror neurons' suggested a neural mechanism for monkeys to share the actions of others. Here we expand this view by showing that mirror neurons for actions not only exist in the premotor cortex or in monkeys and that vicarious activity can also be measured for the emotions and sensations of others. Although we still need to empirically explore the function and development of these vicarious activations, we should stop thinking of vicarious brain activity as a peculiar property of the premotor cortex: instead it seems to be a very common phenomenon which leads social stimuli to recruit a wide range of seemingly private neural systems. PMID:19880311

  17. Corrective Action Investigation Plan for Corrective Action Unit 428: Area 3 Septic Waste Systems 1 and 5, Tonopah Test Range, Nevada, REVISION 0, march 1999

    SciTech Connect

    DOE /NV

    1999-03-26

    and Recovery Act characteristic volatile organic compounds, semivolatile organic compounds, and metals. A limited number of samples will be analyzed for gamma- emitting radionuclides and isotopic uranium from four of the septic tanks and if radiological field screening levels are exceeded. Additional samples will be analyzed for geotechnical and hydrological properties and a bioassessment may be performed. The technical approach for investigating this Corrective Action Unit consists of the following activities: (1) Perform video surveys of the discharge and outfall lines. (2) Collect samples of material in the septic tanks. (3) Conduct exploratory trenching to locate and inspect subsurface components. (4) Collect subsurface soil samples in areas of the collection system including the septic tanks and outfall end of distribution boxes. (5) Collect subsurface soil samples underlying the leachfield distribution pipes via trenching. (6) Collect surface and near- surface samples near potential locations of the Acid Sewer Outfall if Septic Waste System 5 Leachfield cannot be located. (7) Field screen samples for volatile organic compounds, total petroleum hydrocarbons, and radiological activity. (8) Drill boreholes and collect subsurface soil samples if required. (9) Analyze samples for total volatile organic compounds, total semivolatile organic compounds, total Resource Conservation and Recovery Act metals, and total petroleum hydrocarbons (oil/ diesel range organics). Limited number of samples will be analyzed for gamma- emitting radionuclides and isotopic uranium from particular septic tanks and if radiological field screening levels are exceeded. (10) Collect samples from native soils beneath the distribution system and analyze for geotechnical/ hydrologic parameters. (11) Collect and analyze bioassessment samples at the discretion of the Site Supervisor if total petroleum hydrocarbons exceed field- screening levels.

  18. Corrective Action Investigation Plan for Corrective Action Unit 375: Area 30 Buggy Unit Craters, Nevada Test Site, Nevada

    SciTech Connect

    Patrick Matthews

    2010-03-01

    Corrective Action Unit (CAU) 375 is located in Areas 25 and 30 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 375 comprises the two corrective action sites (CASs) listed below: • 25-23-22, Contaminated Soils Site • 30-45-01, U-30a, b, c, d, e Craters Existing information on the nature and extent of potential contamination present at the CAU 375 CASs is insufficient to evaluate and recommend corrective action alternatives (CAAs). This document details an investigation plan that will provide for the gathering of sufficient information to evaluate and recommend CAAs. Corrective Action Site 25-23-22 is composed of the releases associated with nuclear rocket testing at Test Cell A (TCA). Test Cell A was used to test and develop nuclear rocket motors as part of the Nuclear Rocket Development Station from its construction in 1958 until 1966, when rocket testing began being conducted at Test Cell C. The rocket motors were built with an unshielded nuclear reactor that produced as much as 1,100 kilowatts (at full power) to heat liquid hydrogen to 4,000 degrees Fahrenheit, at which time the expanded gases were focused out a nozzle to produce thrust. The fuel rods in the reactor were not clad and were designed to release fission fragments to the atmosphere, but due to vibrations and loss of cooling during some operational tests, fuel fragments in excess of planned releases became entrained in the exhaust and spread in the immediate surrounding area. Cleanup efforts have been undertaken at times to collect the fuel rod fragments and other contamination. Previous environmental investigations in the TCA area have resulted in the creation of a number of use restrictions. The industrial area of TCA is encompassed by a fence and is currently posted as a radioactive material area. Corrective Action Site 30-45-01 (releases associated with the Buggy Plowshare test) is located in Area 30 on Chukar Mesa. It was a

  19. Corrective Action Investigation Plan for Corrective Action Unit 367: Area 10 Sedan, Ess and Uncle Unit Craters Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2009-12-01

    Corrective Action Unit 367 is located in Area 10 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 367 comprises the four corrective action sites (CASs) listed below: • 10-45-01, U-10h Crater (Sedan) • 10-45-02, Ess Crater Site • 10-09-03, Mud Pit • 10-45-03, Uncle Crater Site The CASs in CAU 367 are being investigated because hazardous and/or radioactive contaminants may be present in concentrations that exceed risk-based corrective action (RBCA) levels. Existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend CAAs for the CASs. Additional information will be generated by conducting a CAI before evaluating and selecting CAAs. The scope of the corrective action investigation for CAU 367 includes the following activities: • Move surface debris and/or materials, as needed, to facilitate sampling. • Conduct radiological surveys. • Collect and submit environmental samples for laboratory analysis to determine the area where TED at the site exceeds FALs (i.e., corrective action boundary). • Evaluate TED to potential receptors in areas along Mercury Highway that have been impacted by a release of radionuclides from the Sedan test. • Collect and submit environmental samples for laboratory analysis related to the drilling mud within CAS 10-09-03, Mud Pit, and any encountered stains or waste as necessary to determine whether COCs are present. • If COCs are present, collect additional step-out samples to define the extent of the contamination. • Collect samples of investigation-derived waste, as needed, for waste management purposes.

  20. Treating Another's Actions as One's Own: Children's Memory of and Learning from Joint Activity

    ERIC Educational Resources Information Center

    Sommerville, Jessica A.; Hammond, Amy J.

    2007-01-01

    Children often overestimate their contribution to collaborative activities. Across 2 studies, the authors investigated whether this memory bias supports internalization of the actions of others in the context of joint exchanges. After taking turns with (high collaborative condition; Studies 1 and 2) or working independently of (low collaborative…

  1. Corrective Action Investigation Plan for Corrective Action Unit 151: Septic Systems and Discharge Area, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    David A. Strand

    2004-06-01

    This Corrective Action Investigation Plan (CAIP) contains project-specific information for conducting site investigation activities at Corrective Action Unit (CAU) 151: Septic Systems and Discharge Area, Nevada Test Site, Nevada. Information presented in this CAIP includes facility descriptions, environmental sample collection objectives, and criteria for the selection and evaluation of environmental corrective action alternatives. Corrective Action Unit 151 is located in Areas 2, 12, 18, and 20 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 151 is comprised of the nine Corrective Action Sites (CAS) listed below: (1) 02-05-01, UE-2ce Pond; (2) 12-03-01, Sewage Lagoons (6); (3) 12-04-01, Septic Tanks; (4) 12-04-02, Septic Tanks; (5) 12-04-03, Septic Tank; (6) 12-47-01, Wastewater Pond; (7) 18-03-01, Sewage Lagoon; (8) 18-99-09, Sewer Line (Exposed); and (9) 20-19-02, Photochemical Drain. The CASs within CAU 151 are discharge and collection systems. Corrective Action Site 02-05-01 is located in Area 2 and is a well-water collection pond used as a part of the Nash test. Corrective Action Sites 12-03-01, 12-04-01, 12-04-02, 12-04-03, and 12-47-01 are located in Area 12 and are comprised of sewage lagoons, septic tanks, associated piping, and two sumps. The features are a part of the Area 12 Camp housing and administrative septic systems. Corrective Action Sites 18-03-01 and 18-99-09 are located in the Area 17 Camp in Area 18. These sites are sewage lagoons and associated piping. The origin and terminus of CAS 18-99-09 are unknown; however, the type and configuration of the pipe indicates that it may be a part of the septic systems in Area 18. Corrective Action Site 20-19-02 is located in the Area 20 Camp. This site is comprised of a surface discharge of photoprocessing chemicals.

  2. Action of palmatine on Tetrahymena thermophila BF5 growth investigated by microcalorimetry.

    PubMed

    Kong, Wei-Jun; Zhao, Yan-Ling; Xiao, Xiao-He; Li, Zu-Lun; Ren, Yong-Shen

    2009-09-15

    Using a thermal activity monitor (TAM) air isothermal microcalorimeter with ampoule mode, the thermo-genic curves of the metabolism of Tetrahymena thermophila BF(5) growth at 28 degrees C were obtained and the action of palmatine on it was investigated. Meanwhile, the biomass change during the process of T. thermophila BF(5) growth coexisted with palmatine was studied by a haemacytometer. The results showed that a low concentration (50 microg/mL) of palmatine began to inhibit the growth of T. thermophila BF(5), and when the concentration of palmatine reached 600 microg/mL, T. thermophila BF(5) could not grow at all. The relationship between the growth rate constant (k) and the concentration c was almost linear with the correlation coefficient of 0.9957, showing the strong toxic action of palmatine on T. thermophila BF(5) growth. The biomass during T. thermophila BF(5) growth decreased obviously by the addition of palmatine at different concentrations. The investigation of biomass agreed well with the results obtained by means of microcalorimetry.

  3. Corrective Action Investigation Plan for Corrective Action Unit 490: Station 44 Burn Area, Tonopah Test Range, Nevada (with Record of Technical Change No.1)

    SciTech Connect

    U.S. Department of Energy, Nevada Operations Office

    2000-06-09

    This Corrective Action Investigation Plan (CAIP) contains the U.S. Department of Energy, Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 490 under the Federal Facility Agreement and Consent Order. Corrective Active Unit 490 consists of four Corrective Action Sites (CASs): 03-56-001-03BA, Fire Training Area (FTA); RG-56-001-RGBA, Station 44 Burn Area; 03-58-001-03FN, Sandia Service Yard; and 09-54-001-09L2, Gun Propellant Burn Area. These CASs are located at the Tonopah Test Range near Areas 3 and 9. Historically, the FTA was used for training exercises where tires and wood were ignited with diesel fuel. Records indicate that water and carbon dioxide were the only extinguishing agents used during these training exercises. The Station 44 Burn Area was used for fire training exercises and consisted of two wooden structures. The two burn areas (ignition of tires, wood, and wooden structures with diesel fuel and water) were limited to the building footprints (10 ft by 10 ft each). The Sandia Service Yard was used for storage (i.e., wood, tires, metal, electronic and office equipment, construction debris, and drums of oil/grease) from approximately 1979 to 1993. The Gun Propellant Burn Area was used from the 1960s to 1980s to burn excess artillery gun propellant, solid-fuel rocket motors, black powder, and deteriorated explosives; additionally, the area was used for the disposal of experimental explosive items. Based on site history, the focus of the field investigation activities will be to: (1) determine the presence of contaminants of potential concern (COPCs) at each CAS, (2) determine if any COPCs exceed field-screening levels and/or preliminary action levels, and (3) determine the nature and extent of contamination with enough certainty to support selection of corrective action alternatives for each CAS. The scope of this CAIP is to resolve the

  4. Corrective Action Investigation Plan for Corrective Action Unit 309: Area 12 Muckpiles, Nevada Test Site, Nevada, Rev. No. 0

    SciTech Connect

    Robert F. Boehlecke

    2004-12-01

    This Corrective Action Investigation Plan (CAIP) for Corrective Action Unit (CAU) 309, Area 12 Muckpiles, Nevada Test Site (NTS), Nevada, has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (FFACO) that was agreed to by the State of Nevada, the U.S. Department of Energy, and the U.S. Department of Defense. The general purpose of the investigation is to ensure that adequate data are collected to provide sufficient and reliable information to identify, evaluate, and select technically viable corrective actions. Corrective Action Unit 309 is comprised of the following three corrective action sites (CASs) in Area 12 of the NTS: (1) CAS 12-06-09, Muckpile; (2) CAS 12-08-02, Contaminated Waste Dump (CWD); and (3) CAS 12-28-01, I-, J-, and K-Tunnel Debris. Corrective Action Site 12-06-09 consists of a muckpile and debris located on the hillside in front of the I-, J-, and K-Tunnels on the eastern slopes of Rainier Mesa in Area 12. The muckpile includes mining debris (muck) and debris generated during the excavation and construction of the I-, J-, and K-Tunnels. Corrective Action Site 12-08-02, CWD, consists of a muckpile and debris and is located on the hillside in front of the re-entry tunnel for K-Tunnel. For the purpose of this investigation CAS 12-28-01 is defined as debris ejected by containment failures during the Des Moines and Platte Tests and the associated contamination that is not covered in the two muckpile CASs. This site consists of debris scattered south of the I-, J-, and K-Tunnel muckpiles and extends down the hillside, across the valley, and onto the adjacent hillside to the south. In addition, the site will cover the potential contamination associated with ''ventings'' along the fault, fractures, and various boreholes on the mesa top and face. One conceptual site model was developed for all three CASs to address possible contamination migration pathways associated with CAU 309. The data quality objective (DQO

  5. Corrective Action Investigation Plan for Corrective Action Unit 556: Dry Wells and Surface Release Points Nevada Test Site, Nevada (Draft), Revision 0

    SciTech Connect

    Grant Evenson

    2007-02-01

    Corrective Action Unit  (CAU) 556, Dry Wells and Surface Release Points, is located in Areas 6 and 25 of the Nevada Test Site, 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 556 is comprised of four corrective action sites (CASs) listed below: •06-20-04, National Cementers Dry Well •06-99-09, Birdwell Test Hole •25-60-03, E-MAD Stormwater Discharge and Piping •25-64-01, Vehicle Washdown and Drainage Pit These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document.

  6. Taking Action: A Cross-Modal Investigation of Discourse-Level Representations

    PubMed Central

    Kaiser, Elsi

    2012-01-01

    Segmenting stimuli into events and understanding the relations between those events is crucial for understanding the world. For example, on the linguistic level, successful language use requires the ability to recognize semantic coherence relations between events (e.g., causality, similarity). However, relatively little is known about the mental representation of discourse structure. We report two experiments that used a cross-modal priming paradigm to investigate how humans represent the relations between events. Participants repeated a motor action modeled by the experimenter (e.g., rolled a ball toward mini bowling pins to knock them over), and then completed an unrelated sentence-continuation task (e.g., provided a continuation for “Peter scratched John.…”). In two experiments, we tested whether and how the coherence relations represented by the motor actions (e.g., causal events vs. non-causal events) influence participants’ performance in the linguistic task. (A production study was also conducted to explore potential syntactic priming effects.) Our analyses focused on the coherence relations between the prompt sentences and participants’ continuations, as well as the referential shifts in the continuations. As a whole, the results suggest that the mental representations activated by motor actions overlap with the mental representations used during linguistic discourse-level processing, but nevertheless contain fine-grained information about sub-types of causality (reaction vs. consequence). In addition, the findings point to parallels between shifting one’s attention from one-event to another and shifting one’s attention from one referent to another, and indicate that the event structure of causal sequences is conceptualized more like single events than like two distinct events. As a whole, the results point toward common representations activated by motor sequences and discourse-semantic relations, and further our understanding of the mental

  7. EEG activations during intentional inhibition of voluntary action: an electrophysiological correlate of self-control?

    PubMed

    Walsh, E; Kühn, S; Brass, M; Wenke, D; Haggard, P

    2010-01-01

    An important aspect of volition is the internal decision whether to act or to withhold an action. We used EEG frequency analysis of sensorimotor rhythms to investigate brain activity when people prepare and then cancel a voluntary action. Participants used a rotating clock-hand to report when they experienced the intention to press a key with their right hand, even on trials where they freely decided to inhibit movement at the last moment. On action trials, we observed the classical pattern of reduced beta-band spectral power prior to movement, followed by beta rebound after movement. On inhibition trials where participants prepared but then cancelled a movement, we found a left frontal increase in spectral power (event-related synchronisation: ERS) peaking 12 ms before the perceived intention to move. This neural correlate of intentional inhibition was significantly different from the activity at the corresponding moment in action trials. The results are discussed in the context of a recent model of voluntary action (WWW model; Brass & Haggard, 2008). Planned actions can be subjected to a final predictive check which either commits actions for execution or suspends and withholds them. The neural mechanism of intentional inhibition may play an important role in self-control.

  8. Corrective Action Investigation Plan for Corrective Action Unit 266: Area 25 Building 3124 Leachfield, Nevada Test Site, Nevada, Revision 1, February 1999

    SciTech Connect

    U.S. Department Of Energy, Nevada Operations Office

    1999-02-24

    The Corrective Action Investigation Plan for Corrective Action Unit 266, Area 25 Building 3124 Leachfield, has been developed in accordance with the Federal Facility Agreement and Consent Order that was agreed to by the U.S. Department of Energy, Nevada Operations Office; the State of Nevada Division of Environmental Protection; and the U.S. Department of Defense. Corrective Action Unit 266 consists of the Corrective Action Site 25-05-09 sanitary leachfield and associated collection system. This Corrective Action Investigation Plan is used in combination with the Work Plan for Leachfield Corrective Action Units: Nevada Test Site and Tonopah Test Range, Nevada (DOE/NV, 1998d). This Corrective Action Investigation Plan provides investigative details specific to Corrective Action Unit 266. Corrective Action Unit 266 is located southwest of Building 3124 which is located southwest and adjacent to Test Cell A. Test Cell A was operational during the 1960s to test nuclear rocket reactors in support of the Nuclear Rocket Development Station. Operations within Building 3124 from 1962 through the early 1990s resulted in effluent releases to the leachfield and associated collection system. The subsurface soils in the vicinity of the collection system and leachfield may have been impacted by effluent containing contaminants of potential concern generated by support activities associated with Test Cell A reactor testing operations, various laboratories including a high-level radioactivity environmental sample handling laboratory, and possibly the Treatability Test Facility. Based on site history collected to support the Data Quality Objectives process, contaminants of potential concern for the site include radionuclides, oil/diesel range total petroleum hydrocarbons, and Resource Conservation and Recovery Act characteristic volatile organic compounds, semivolatile organic compounds, and metals. Samples will also be analyzed for radionuclides and polychlorinated biphenyls not

  9. Corrective Action Investigation Plan for Corrective Action Unit 366: Area 11 Plutonium Valley Dispersion Sites, Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2011-09-01

    associated with the nuclear tests, it was determined that CASs 11-23-02, 11-23-03, and 11-23-04 will be investigated as one release site. The three test areas associated with these CASs are in close proximity; the devices tested were all composed of plutonium and enriched uranium; and the ground zeroes are all posted high contamination areas (HCAs). Because the device tested at CAS 11-23-01 was composed primarily of enriched uranium and the ground zero is not a posted HCA, the CAS will be investigated as a separate release. The DQO process also resulted in an assumption that TED within the HCAs and contaminated waste dumps exceeds the FAL and requires corrective action. A field investigation will be performed to define where TED exceeds the FAL and to determine whether other contaminants of concern are present at the site associated with other activities that took place at the site or from spills or waste discovered during the investigation. The presence and nature of contamination from other types of releases (such as migration and any potential releases discovered during the investigation) will be evaluated using soil samples collected from the locations most likely containing contamination, if present. Appendix A provides a detailed discussion of the DQO methodology and the DQOs specific to each CAS.

  10. Current activities handbook: formerly utilized sites remedial action program

    SciTech Connect

    1981-02-27

    This volume is one of a series produced under contract with the DOE, by Politech Corporation to develop a legislative and regulatory data base to assist the FUSRAP management in addressing the institutional and socioeconomic issues involved in carrying out the Formerly Utilized Sites Remedial Action Program. This Information Handbook series contains information about all relevant government agencies at the Federal and state levels, the pertinent programs they administer, each affected state legislature, and current Federal and state legislative and regulatory initiatives. This volume is a compilation of information about the activities each of the thirteen state legislatures potentially affected by the Formerly Utilized Sites Remedial Action Program. It contains a description of the state legislative procedural rules and a schedule of each legislative session; a summary of pending relevant legislation; the name and telephone number of legislative and state agency contacts; and the full text of all bills identified.

  11. Corrective Action Investigation Plan for Corrective Action Unit 34: Area 3 Contaminated Waste Site, Nevada Test Site, Nevada (Rev. 0, March 2001)

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office

    2001-03-27

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 34 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 34 consists of four Corrective Action Sites (CASs). The CAU is located within the Area 3 Compound at the Nevada Test Site (NTS) in the vicinity of the Mud Plant Facility in Yucca Valley. Historically, CAS 03-09-07, Mud Pit, was used for disposal of excess mud from washing drilling equipment from 1968 to 1974, at which time it began to be used for excess mud disposal (currently inactive); CAS 03-44-01, Chromium Contamination Spill, was used to store additives used in the formulation of drilling mud from the early 1960s to the mid-1990s; CAS 03-47-02, Area 3 Mud Plant Pond, was used as a freshwater storage reservoir for the mud plant as well as supplied water for a number of activities including the mixing of mud, the rinsing and cleaning of tanks, and various washdowns from the 1960s through 1990s; and CAS 03-09-06, Mud Disposal Crater, was created in 1962 by an underground nuclear detonation (i.e., Chinchilla test) and was used to mix and store mud, dispose of receiving waste from the mud plant floor drains and excess drilling mud, and clean/flush mix tanks through the mid-1990s. Based on site history, the scope of this plan is to identify potentially contaminated ground soil at each of the four CASs and determine the quantity, nature, and extent of contaminants of potential concern (COPCs). The investigation will include systematic and biased surface and subsurface soil and mud sampling using hand-auguring and direct-push techniques; visual, video, and/or electromagnetic surveys of pipes; field screening for volatile organic compounds (VOCs) and alpha/beta-emitting radionuclides; and laboratory

  12. Corrective Action Investigation Plan for Corrective Action Unit 219: Septic Systems and Injection Wells, Nevada Test Site, Nevada, Rev. No.: 0

    SciTech Connect

    David A. Strand

    2005-01-01

    The Corrective Action Investigation Plan for Corrective Action Unit 219, Septic Systems and Injection Wells, has been developed in accordance with the ''Federal Facility Agreement and Consent Order'' (1996) that was agreed to by the State of Nevada, the U.S. Department of Energy, and the U.S. Department of Defense. The purpose of the investigation is to ensure that adequate data are collected to provide sufficient and reliable information to identify, evaluate, and select technically viable corrective actions. Corrective Action Unit 219 is located in Areas 3, 16, and 23 of the Nevada Test Site, which is 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 219 is comprised of the six Corrective Action Sites (CASs) listed below: (1) 03-11-01, Steam Pipes and Asbestos Tiles; (2) 16-04-01, Septic Tanks (3); (3) 16-04-02, Distribution Box; (4) 16-04-03, Sewer Pipes; (5) 23-20-01, DNA Motor Pool Sewage and Waste System; and (6) 23-20-02, Injection Well. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation prior to evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document.

  13. Corrective Action Investigation Plan for Corrective Action Unit 567: Miscellaneous Soil Sites, Nevada National Security Site, Nevada, with ROTC 1 Revision 0

    SciTech Connect

    Matthews, Patrick K.

    2013-07-01

    Corrective Action Unit (CAU) 567 is located in Areas 1, 3, 5, 20, and 25 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. CAU 567 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 567, which comprises the following corrective action sites (CASs): • 01-23-03, Atmospheric Test Site T-1 • 03-23-25, Seaweed E Contamination Area • 05-23-07, A5b RMA • 20-23-08, Colby Mud Spill • 25-23-23, J-11 Soil RMA These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the investigation report. The sites will be investigated based on the data quality objectives (DQOs) developed on May 6, 2013, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Field Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 567. The site investigation process will also be conducted in accordance with the Soils Activity Quality Assurance Plan, which establishes requirements, technical planning, and general quality practices to be applied to this activity. The potential contamination sources associated with CAU 567 releases are nuclear test operations and other NNSS operations. The DQO process resulted in an assumption that total effective dose (TED) within a default contamination boundary

  14. Action!

    ERIC Educational Resources Information Center

    Senese, Joseph

    1998-01-01

    A small group of teachers at one Illinois high school is helping to effect and promote change. Through the Action Research Laboratory (ARL), teams of teachers conduct collaborative action research to improve classroom practices. Data from the first two years of the ARL indicate that teachers are eager to participate in, and have thrived in, their…

  15. Corrective Action Investigation Plan for Corrective Action Unit 262: Area 25 Septic Systems and Underground Discharge Point, Nevada Test Site, Nevada, Revision No. 1 (9/2001)

    SciTech Connect

    NNSA /NV

    2000-07-20

    This corrective action investigation plan contains the U.S. Department of Energy, Nevada Operations Office's approach to collect data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 262 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 262 consists of nine Corrective Action Sites (CASs): Underground Storage Tank (25-02-06), Septic Systems A and B (25-04-06), Septic System (25-04-07), Leachfield (25-05-03), Leachfield (25-05-05), Leachfield (25-05-06), Radioactive Leachfield (25-05-08), Leachfield (25-05-12), and Dry Well (25-51-01). Situated in Area 25 at the Nevada Test Site (NTS), sites addressed by CAU 262 are located at the Reactor-Maintenance, Assembly, and Disassembly (R-MAD); Test Cell C; and Engine-Maintenance, Assembly, and Disassembly (E-MAD) facilities. The R-MAD, Test Cell C, and E-MAD facilities supported nuclear rocket reactor and engine testing as part of the Nuclear Rocket Development Station. The activities associated with the testing program were conducted between 1958 and 1973. Based on site history collected to support the Data Quality Objectives process, contaminants of potential concern (COPCs) for the site include oil/diesel-range total petroleum hydrocarbons, volatile organic compounds, semivolatile organic compounds, polychlorinated biphenyls, Resource Conservation and Recovery Act metals, and gamma-emitting radionuclides, isotopic uranium, isotopic plutonium, strontium-90, and tritium. The scope of the corrective action field investigation at the CAU will include the inspection of portions of the collection systems, sampling the contents of collection system features in situ of leachfield logging materials, surface soil sampling, collection of samples of soil underlying the base of inlet and outfall ends of septic tanks and outfall ends of diversion structures and distribution boxes, collection of soil samples from biased or a combination of

  16. Investigating a Potential Auxin-Related Mode of Hormetic/Inhibitory Action of the Phytotoxin Parthenin.

    PubMed

    Belz, Regina G

    2016-01-01

    Parthenin is a metabolite of Parthenium hysterophorus and is believed to contribute to the weed's invasiveness via allelopathy. Despite the potential of parthenin to suppress competitors, low doses stimulate plant growth. This biphasic action was hypothesized to be auxin-like and, therefore, an auxin-related mode of parthenin action was investigated using two approaches: joint action experiments with Lactuca sativa, and dose-response experiments with auxin/antiauxin-resistant Arabidopsis thaliana genotypes. The joint action approach comprised binary mixtures of subinhibitory doses of the auxin 3-indoleacetic acid (IAA) mixed with parthenin or one of three reference compounds [indole-3-butyric acid (IBA), 2,3,5-triiodobenzoic acid (TIBA), 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB)]. The reference compounds significantly interacted with IAA at all doses, but parthenin interacted only at low doses indicating that parthenin hormesis may be auxin-related, in contrast to its inhibitory action. The genetic approach investigated the response of four auxin/antiauxin-resistant mutants and a wildtype to parthenin or two reference compounds (IAA, PCIB). The responses of mutant plants to the reference compounds confirmed previous reports, but differed from the responses observed for parthenin. Parthenin stimulated and inhibited all mutants independent of resistance. This provided no indication for an auxin-related action of parthenin. Therefore, the hypothesis of an auxin-related inhibitory action of parthenin was rejected in two independent experimental approaches, while the hypothesis of an auxin-related stimulatory effect could not be rejected.

  17. A behavioral task for investigating action discovery, selection and switching: comparison between types of reinforcer

    PubMed Central

    Fisher, Simon D.; Gray, Jason P.; Black, Melony J.; Davies, Jennifer R.; Bednark, Jeffery G.; Redgrave, Peter; Franz, Elizabeth A.; Abraham, Wickliffe C.; Reynolds, John N. J.

    2014-01-01

    Action discovery and selection are critical cognitive processes that are understudied at the cellular and systems neuroscience levels. Presented here is a new rodent joystick task suitable to test these processes due to the range of action possibilities that can be learnt while performing the task. Rats learned to manipulate a joystick while progressing through task milestones that required increasing degrees of movement accuracy. In a switching phase designed to measure action discovery, rats were repeatedly required to discover new target positions to meet changing task demands. Behavior was compared using both food and electrical brain stimulation reward (BSR) of the substantia nigra as reinforcement. Rats reinforced with food and those with BSR performed similarly overall, although BSR-treated rats exhibited greater vigor in responding. In the switching phase, rats learnt new actions to adapt to changing task demands, reflecting action discovery processes. Because subjects are required to learn different goal-directed actions, this task could be employed in further investigations of the cellular mechanisms of action discovery and selection. Additionally, this task could be used to assess the behavioral flexibility impairments seen in conditions such as Parkinson's disease and obsessive-compulsive disorder. The versatility of the task will enable cross-species investigations of these impairments. PMID:25477795

  18. Investigating a Potential Auxin-Related Mode of Hormetic/Inhibitory Action of the Phytotoxin Parthenin.

    PubMed

    Belz, Regina G

    2016-01-01

    Parthenin is a metabolite of Parthenium hysterophorus and is believed to contribute to the weed's invasiveness via allelopathy. Despite the potential of parthenin to suppress competitors, low doses stimulate plant growth. This biphasic action was hypothesized to be auxin-like and, therefore, an auxin-related mode of parthenin action was investigated using two approaches: joint action experiments with Lactuca sativa, and dose-response experiments with auxin/antiauxin-resistant Arabidopsis thaliana genotypes. The joint action approach comprised binary mixtures of subinhibitory doses of the auxin 3-indoleacetic acid (IAA) mixed with parthenin or one of three reference compounds [indole-3-butyric acid (IBA), 2,3,5-triiodobenzoic acid (TIBA), 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB)]. The reference compounds significantly interacted with IAA at all doses, but parthenin interacted only at low doses indicating that parthenin hormesis may be auxin-related, in contrast to its inhibitory action. The genetic approach investigated the response of four auxin/antiauxin-resistant mutants and a wildtype to parthenin or two reference compounds (IAA, PCIB). The responses of mutant plants to the reference compounds confirmed previous reports, but differed from the responses observed for parthenin. Parthenin stimulated and inhibited all mutants independent of resistance. This provided no indication for an auxin-related action of parthenin. Therefore, the hypothesis of an auxin-related inhibitory action of parthenin was rejected in two independent experimental approaches, while the hypothesis of an auxin-related stimulatory effect could not be rejected. PMID:26686984

  19. Corrective Action Investigation Plan for Corrective Action Unit 529: Area 25 Contaminated Materials, Nevada Test Site, Nevada, Rev. 0, Including Record of Technical Change No. 1

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2003-02-26

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 529, Area 25 Contaminated Materials, Nevada Test Site (NTS), Nevada, under the Federal Facility Agreement and Consent Order. CAU 529 consists of one Corrective Action Site (25-23-17). For the purpose of this investigation, the Corrective Action Site has been divided into nine parcels based on the separate and distinct releases. A conceptual site model was developed for each parcel to address the translocation of contaminants from each release. The results of this investigation will be used to support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  20. What are you doing? How active and observational experience shape infants' action understanding

    PubMed Central

    Hunnius, Sabine; Bekkering, Harold

    2014-01-01

    From early in life, infants watch other people's actions. How do young infants come to make sense of actions they observe? Here, we review empirical findings on the development of action understanding in infancy. Based on this review, we argue that active action experience is crucial for infants' developing action understanding. When infants execute actions, they form associations between motor acts and the sensory consequences of these acts. When infants subsequently observe these actions in others, they can use their motor system to predict the outcome of the ongoing actions. Also, infants come to an understanding of others’ actions through the repeated observation of actions and the effects associated with them. In their daily lives, infants have plenty of opportunities to form associations between observed events and learn about statistical regularities of others’ behaviours. We argue that based on these two forms of experience—active action experience and observational experience—infants gradually develop more complex action understanding capabilities. PMID:24778386

  1. Dynamic structure of joint-action stimulus-response activity.

    PubMed

    Malone, MaryLauren; Castillo, Ramon D; Kloos, Heidi; Holden, John G; Richardson, Michael J

    2014-01-01

    The mere presence of a co-actor can influence an individual's response behavior. For instance, a social Simon effect has been observed when two individuals perform a Go/No-Go response to one of two stimuli in the presence of each other, but not when they perform the same task alone. Such effects are argued to provide evidence that individuals co-represent the task goals and the to-be-performed actions of a co-actor. Motivated by the complex-systems approach, the present study was designed to investigate an alternative hypothesis--that such joint-action effects are due to a dynamical (time-evolving) interpersonal coupling that operates to perturb the behavior of socially situated actors. To investigate this possibility, participants performed a standard Go/No-Go Simon task in joint and individual conditions. The dynamic structure of recorded reaction times was examined using fractal statistics and instantaneous cross-correlation. Consistent with our hypothesis that participants responding in a shared space would become behaviorally coupled, the analyses revealed that reaction times in the joint condition displayed decreased fractal structure (indicative of interpersonal perturbation processes modulating ongoing participant behavior) compared to the individual condition, and were more correlated across a range of time-scales compared to the reaction times of pseudo-pair controls. Collectively, the findings imply that dynamic processes might underlie social stimulus-response compatibility effects and shape joint cognitive processes in general.

  2. [Correlative interconnections between impulse activity of aminergic neurons of the brainstem and spectral components of electroencephalogram during action of bemitil].

    PubMed

    Kolotilova, O I; Pavlenko, V B; Koreniuk, I I; Kulychenko, O M; Fokina, Iu O

    2007-01-01

    Correlative interconnections between frequency of impulse activity of aminergic neurons and neocortex electrical activity during action of bemitil (50 mg/kg) were investigated in 5 cats. It was shown that bemitil affects correlations between frequency of impulses of aminergic neurons and electrical activity of neocortex.

  3. Corrective action investigation plan for Project Shoal Area CAU No. 416

    SciTech Connect

    1996-08-01

    This Corrective Action Investigation Plan (CAIP) is part of an ongoing US Department of Energy (DOE)-funded project for the investigation of Corrective Action Unit (CAU) No. 416, Project Shoal Area (PSA). Project Shoal was conducted to determine whether seismic waves produced by underground nuclear testing could be differentiated from naturally occurring earthquakes. The PSA site is located approximately 30 miles southeast of Fallon, Nevada, in the northern portion of Sand Springs Mountains in Churchill County. This CAIP will be implemented in accordance with the Federal Facility Agreement and Consent Order, the Industrial Sites Quality Assurance Project Plan, and all applicable Nevada Division of Environmental Protection policies and regulations.

  4. Corrective Action Investigation Plan: Roller Coaster Lagoons and North Disposal Trench, Tonopah Test Range, Revision 1

    SciTech Connect

    IT Corporation, Las Vegas, NV

    1996-06-27

    1.1 Purpose The purpose of this investigation is to collect data to confirm the presence or absence of contamination, evaluate the potential for contaminant migration, and select appropriate closure methods for these sites. The potential closure methods for these sites involve either clean closure, closure in place, or no further action. 1.2 Scope The scope of this investigation includes collecting surface and subsurface soil samples at the Roller Coaster Lagoons; and collecting surface soil samples at the North Disposal Trench and the small spill area associated with the Voluntary Cotiective Action (VCA) that was conducted in 1995.

  5. Math Activities Using LogoWriter--Investigations.

    ERIC Educational Resources Information Center

    Flewelling, Gary

    This book is one in a series of teacher resource books developed to: (1) rescue students from the clutches of computers that drill and control; and (2) supply teachers with computer activities compatible with a mathematics program that emphasizes investigation, problem solving, creativity, and hypothesis making and testing. This is not a book…

  6. Corrective Action Investigation Plan for Corrective Action Unit 372: Area 20 Cabriolet/Palanquin Unit Craters Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2009-06-01

    Corrective Action Unit (CAU) 372 is located in Areas 18 and 20 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 372 is comprised of the four corrective action sites (CASs) listed below: • 18-45-02, Little Feller I Surface Crater • 18-45-03, Little Feller II Surface Crater • 20-23-01, U-20k Contamination Area • 20-45-01, U-20L Crater (Cabriolet) These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. Additional information will be obtained by conducting a corrective action investigation (CAI) before evaluating corrective action alternatives and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on February 10, 2009, by representatives of the Nevada Division of Environmental Protection; U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Stoller-Navarro Joint Venture; Desert Research Institute, and National Security Technologies, LLC. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 372.

  7. Corrective Action Investigation Plan for Corrective Action Unit 536: Area 3 Release Site, Nevada Test Site, Nevada (Rev. 0 / June 2003), Including Record of Technical Change No. 1

    SciTech Connect

    2003-06-27

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach to collect the data necessary to evaluate corrective action alternatives (CAAs) appropriate for the closure of Corrective Action Unit (CAU) 536: Area 3 Release Site, Nevada Test Site, Nevada, under the Federal Facility Agreement and Consent Order. Corrective Action Unit 536 consists of a single Corrective Action Site (CAS): 03-44-02, Steam Jenny Discharge. The CAU 536 site is being investigated because existing information on the nature and extent of possible contamination is insufficient to evaluate and recommend corrective action alternatives for CAS 03-44-02. The additional information will be obtained by conducting a corrective action investigation (CAI) prior to evaluating CAAs and selecting the appropriate corrective action for this CAS. The results of this field investigation are to be used to support a defensible evaluation of corrective action alternatives in the corrective action decision document. Record of Technical Change No. 1 is dated 3-2004.

  8. Mechanisms of insulin action on sympathetic nerve activity

    NASA Technical Reports Server (NTRS)

    Muntzel, Martin S.; Anderson, Erling A.; Johnson, Alan Kim; Mark, Allyn L.

    1996-01-01

    Insulin resistance and hyperinsulinemia may contribute to the development of arterial hypertension. Although insulin may elevate arterial pressure, in part, through activation of the sympathetic nervous system, the sites and mechanisms of insulin-induced sympathetic excitation remain uncertain. While sympathoexcitation during insulin may be mediated by the baroreflex, or by modulation of norepinephrine release from sympathetic nerve endings, it has been shown repeatedly that insulin increases sympathetic outflow by actions on the central nervous system. Previous studies employing norepinephrine turnover have suggested that insulin causes sympathoexcitation by acting in the hypothalamus. Recent experiments from our laboratory involving direct measurements of regional sympathetic nerve activity have provided further evidence that insulin acts in the central nervous system. For example, administration of insulin into the third cerebralventricle increased lumbar but not renal or adrenal sympathetic nerve activity in normotensive rats. Interestingly, this pattern of regional sympathetic nerve responses to central neural administration of insulin is similar to that seen with systemic administration of insulin. Further, lesions of the anteroventral third ventricle hypothalamic (AV3V) region abolished increases in sympathetic activity to systemic administration of insulin with euglycemic clamp, suggesting that AV3V-related structures are critical for insulin-induced elevations in sympathetic outflow.

  9. Corrective Action Investigation Plan for Corrective Action Unit 263: Area 25 Building 4839 Leachfields, Nevada Test Site, Revision 0, DOE/NV--535 UPDATED WITH RECORD OF TECHNICAL CHANGE No.1

    SciTech Connect

    US DOE Nevada Operations Office

    1999-04-12

    The Corrective Action Investigation Plan for Corrective Action Unit 263, the Area 25 Building 4839 Leachfield, has been developed in accordance with the Federal Facility Agreement and Consent Order that was agreed to by the US Department of Energy, Nevada Operations Office; the Nevada Division of Environmental Protection; and the US Department of Defense. Corrective Action Unit 263 is comprised of the Corrective Action Site 25-05-04 sanitary leachfield and associated collection system. This Corrective Action Investigation Plan is used in combination with the Work Plan for Leachfield Corrective Action Units: Nevada Test Site and Tonopah Test Range, Nevada (DOE/NV, 1998d). The Leachfield Work Plan was developed to streamline investigations at Leachfield Corrective Action Units by incorporating management, technical, quality assurance, health and safety, public involvement, field sampling, and waste management information common to a set of Corrective Action Units with similar site histories and characteristics into a single document that can be referenced. This Corrective Action Investigation Plan provides investigative details specific to Corrective Action Unit 263. Corrective Action Unit 263 is located southwest of Building 4839, in the Central Propellant Storage Area. Operations in Building 4839 from 1968 to 1996 resulted in effluent releases to the leachfield and associated collection system. In general, effluent released to the leachfield consisted of sanitary wastewater from a toilet, urinal, lavatory, and drinking fountain located within Building 4839. The subsurface soils in the vicinity of the collection system and leachfield may have been impacted by effluent containing contaminants of potential concern generated by support activities associated with the Building 4839 operations.

  10. Glycosylated antitumor ether lipids: activity and mechanism of action.

    PubMed

    Arthur, Gilbert; Bittman, Robert

    2014-05-01

    Glycosylated antitumor ether lipids (GAELs) are distinguished from the alkyllysophospholipids or alkylphosphocholines classes of antitumor ether lipids (AEL) by the presence of a sugar moiety. Non-phosphorus GAELs, the subject of this review, have a sugar moiety in place of the phosphobase found in alkyllysophospholipids. Analogues of non-phosphorus GAELs with glucose, maltose, arabinose, or disaccharide moieties have been synthesized. Non-phosphorus GAELs with monosaccharides have cytotoxic and antiproliferative effects against cancer cells derived from a wide range of tissues, including drug resistant cell lines. The most active compound of this group to date is 1-O-hexadecyl-2-O-methyl-3-O-(2'-amino-2'-deoxy-β-D-glucopyranosyl)-sn-glycerol (11), which displays in vitro activity similar to or greater than that of ET-18-OCH3, the AEL "gold" standard. While the detailed molecular mechanism of action of non-phosphorus GAELs is not known, the data indicate that non-phosphorus GAELs are taken up by endocytosis and incorporated into early endosomes. The presence of non-phosphorus GAELs perturbs the maturation of the endocytic vesicles, resulting in the formation of large acidic vacuoles. Cell death appears to be the result of the release of cathepsins from the vacuoles into the cytosol and subsequent activation of a death pathway that is independent of the mitochondria and independent of apoptosis. The ability of these GAELs to kill cells via an apoptosis-independent mechanism makes them prime candidates for development of effective compounds against chemo-resistant tumors and cancer stem cells. The disaccharide-linked GAELs do not have cytotoxic activity but rather inhibit cancer cell motility due to the ability of the compounds to block specific calcium-activated potassium channels in cells. The antitumor activities displayed by these experimental compounds augurs well for their eventual development into clinically useful agents for cancer treatment.

  11. Sandia's activities in uranium mill tailings remedial action

    SciTech Connect

    Neuhauser, S.

    1980-01-01

    The Uranium Mill Tailings Radiation Control Act of 1978 requires that remedial action be taken at over 20 inactive uranium mill tailings sites in the United States. Standards promulgated by the EPA under this act are to be the operative standards for this activity. Proposed standards must still undergo internal review, public comment, and receive Nuclear Regulatory Commission concurrence before being finalized. Briefly reviewed, the standards deal separately with new disposal sites (Part A) and cleanup of soil and contaminated structures at existing locations (Part B). In several cases, the present sites are felt to be too close to human habitations or to be otherwise unacceptably located. These tailings will probably be relocated. New disposal sites for relocated tailings must satisfy certain standards. The salient features of these standards are summarized.

  12. Action of methotrexate and tofacitinib on directly stimulated and bystander-activated lymphocytes.

    PubMed

    Piscianz, Elisa; Candilera, Vanessa; Valencic, Erica; Loganes, Claudia; Paron, Greta; De Iudicibus, Sara; Decorti, Giuliana; Tommasini, Alberto

    2016-07-01

    Chronic inflammation associated with autoimmune activation is characteristic of rheumatic diseases from childhood to adulthood. In recent decades, significant improvements in the treatment of these types of disease have been achieved using disease modifying anti-rheumatic drugs (DMARDs), such as methotrexate (MTX) and, more recently, using biologic inhibitors. The recent introduction of kinase inhibitors (for example, tofacitinib; Tofa) further increases the available ARDs. However, there are patients that do not respond to any treatment strategies, for whom combination therapies are proposed. The data regarding the combined action of different drugs is lacking and the knowledge of the mechanisms of ARDs and their actions upon pathogenic lymphocytes, which are hypothesized to sustain disease, is poor. An in vitro model of inflammation was developed in the current study, in which stimulated and unstimulated lymphocytes were cultured together, but tracked separately, to investigate the action of MTX and Tofa on the two populations. By analysing lymphocyte proliferation and activation, and cytokine secretion in the culture supernatants, it was established that, due to the presence of activated cells, unstimulated cells underwent a bystander activation that was modulated by the ARDs. Additionally, varying administration schedules were demonstrated to affect lymphocytes differently in vitro, either directly or via bystander activation. Furthermore, MTX and Tofa exerted different effects; while MTX showed an antiproliferative effect, Tofa marginally effected activation, although only a slight antiproliferative action, which could be potentiated by sequential treatment with MTX. Thus, it was hypothesized that these differences may be exploited in sequential therapeutic strategies, to maximize the anti‑rheumatic effect. These findings are notable and must be accounted for, as bystander‑activated cells in vivo could contribute to the spread of autoimmune activation

  13. Investigating the Impact of Possession-Way of a Smartphone on Action Recognition.

    PubMed

    Kim, Zae Myung; Jeong, Young-Seob; Oh, Hyung Rai; Oh, Kyo-Joong; Lim, Chae-Gyun; Iraqi, Youssef; Choi, Ho-Jin

    2016-01-01

    For the past few decades, action recognition has been attracting many researchers due to its wide use in a variety of applications. Especially with the increasing number of smartphone users, many studies have been conducted using sensors within a smartphone. However, a lot of these studies assume that the users carry the device in specific ways such as by hand, in a pocket, in a bag, etc. This paper investigates the impact of providing an action recognition system with the information of the possession-way of a smartphone, and vice versa. The experimental dataset consists of five possession-ways (hand, backpack, upper-pocket, lower-pocket, and shoulder-bag) and two actions (walking and running) gathered by seven users separately. Various machine learning models including recurrent neural network architectures are employed to explore the relationship between the action recognition and the possession-way recognition. The experimental results show that the assumption of possession-ways of smartphones do affect the performance of action recognition, and vice versa. The results also reveal that a good performance is achieved when both actions and possession-ways are recognized simultaneously.

  14. Investigating the Impact of Possession-Way of a Smartphone on Action Recognition.

    PubMed

    Kim, Zae Myung; Jeong, Young-Seob; Oh, Hyung Rai; Oh, Kyo-Joong; Lim, Chae-Gyun; Iraqi, Youssef; Choi, Ho-Jin

    2016-01-01

    For the past few decades, action recognition has been attracting many researchers due to its wide use in a variety of applications. Especially with the increasing number of smartphone users, many studies have been conducted using sensors within a smartphone. However, a lot of these studies assume that the users carry the device in specific ways such as by hand, in a pocket, in a bag, etc. This paper investigates the impact of providing an action recognition system with the information of the possession-way of a smartphone, and vice versa. The experimental dataset consists of five possession-ways (hand, backpack, upper-pocket, lower-pocket, and shoulder-bag) and two actions (walking and running) gathered by seven users separately. Various machine learning models including recurrent neural network architectures are employed to explore the relationship between the action recognition and the possession-way recognition. The experimental results show that the assumption of possession-ways of smartphones do affect the performance of action recognition, and vice versa. The results also reveal that a good performance is achieved when both actions and possession-ways are recognized simultaneously. PMID:27271623

  15. Investigating the Impact of Possession-Way of a Smartphone on Action Recognition †

    PubMed Central

    Kim, Zae Myung; Jeong, Young-Seob; Oh, Hyung Rai; Oh, Kyo-Joong; Lim, Chae-Gyun; Iraqi, Youssef; Choi, Ho-Jin

    2016-01-01

    For the past few decades, action recognition has been attracting many researchers due to its wide use in a variety of applications. Especially with the increasing number of smartphone users, many studies have been conducted using sensors within a smartphone. However, a lot of these studies assume that the users carry the device in specific ways such as by hand, in a pocket, in a bag, etc. This paper investigates the impact of providing an action recognition system with the information of the possession-way of a smartphone, and vice versa. The experimental dataset consists of five possession-ways (hand, backpack, upper-pocket, lower-pocket, and shoulder-bag) and two actions (walking and running) gathered by seven users separately. Various machine learning models including recurrent neural network architectures are employed to explore the relationship between the action recognition and the possession-way recognition. The experimental results show that the assumption of possession-ways of smartphones do affect the performance of action recognition, and vice versa. The results also reveal that a good performance is achieved when both actions and possession-ways are recognized simultaneously. PMID:27271623

  16. Action Learning in Higher Education: An Investigation of Its Potential to Develop Professional Capability

    ERIC Educational Resources Information Center

    Lizzio, Alf; Wilson, Keithia

    2004-01-01

    This study investigated the extent to which a course, designed using peer and action learning principles to function as an 'on campus practicum', can develop the professional capabilities of students. As part of their formal coursework, third year behavioural science students, functioning as 'student consultants', entered into a…

  17. 29 CFR 801.20 - Adverse employment action under ongoing investigation exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Restrictions on Polygraph Usage Under Exemptions § 801.20 Adverse employment action under ongoing investigation exemption... upon the analysis of a polygraph test chart or the refusal to take a polygraph test, without...

  18. 29 CFR 801.20 - Adverse employment action under ongoing investigation exemption.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Restrictions on Polygraph Usage Under Exemptions § 801.20 Adverse employment action under ongoing investigation exemption... upon the analysis of a polygraph test chart or the refusal to take a polygraph test, without...

  19. 29 CFR 801.20 - Adverse employment action under ongoing investigation exemption.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Restrictions on Polygraph Usage Under Exemptions § 801.20 Adverse employment action under ongoing investigation exemption... upon the analysis of a polygraph test chart or the refusal to take a polygraph test, without...

  20. 29 CFR 801.20 - Adverse employment action under ongoing investigation exemption.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Restrictions on Polygraph Usage Under Exemptions § 801.20 Adverse employment action under ongoing investigation exemption... upon the analysis of a polygraph test chart or the refusal to take a polygraph test, without...

  1. 29 CFR 801.20 - Adverse employment action under ongoing investigation exemption.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., DEPARTMENT OF LABOR OTHER LAWS APPLICATION OF THE EMPLOYEE POLYGRAPH PROTECTION ACT OF 1988 Restrictions on Polygraph Usage Under Exemptions § 801.20 Adverse employment action under ongoing investigation exemption... upon the analysis of a polygraph test chart or the refusal to take a polygraph test, without...

  2. The Effects of Issue Investigation and Action Training on Environmental Behavior in Seventh Grade Students.

    ERIC Educational Resources Information Center

    Ramsey, John M.; Hungerford, Harold

    1989-01-01

    This study reports the instructional effects of issue investigation and action training on the environmental behavior of middle school students. Discussed are the methodology including training and modules, the design of the study, instruments used, scoring protocols, and analysis of results. (Author/CW)

  3. A Study of the Qualities Teachers Recommend in STS Issues Investigation and Action Instructional Materials.

    ERIC Educational Resources Information Center

    Rubba, Peter A.; Wiesenmayer, Randall L.

    This study was part of a project to develop science and technology (STS) issue investigation and action materials with the potential for use across science and social studies classes at the secondary level. Special emphasis was given to learning teachers' perspectives on the development of a unit on the impact of pesticides on society. Seven areas…

  4. Feature activation during word recognition: action, visual, and associative-semantic priming effects

    PubMed Central

    Lam, Kevin J. Y.; Dijkstra, Ton; Rueschemeyer, Shirley-Ann

    2015-01-01

    Embodied theories of language postulate that language meaning is stored in modality-specific brain areas generally involved in perception and action in the real world. However, the temporal dynamics of the interaction between modality-specific information and lexical-semantic processing remain unclear. We investigated the relative timing at which two types of modality-specific information (action-based and visual-form information) contribute to lexical-semantic comprehension. To this end, we applied a behavioral priming paradigm in which prime and target words were related with respect to (1) action features, (2) visual features, or (3) semantically associative information. Using a Go/No-Go lexical decision task, priming effects were measured across four different inter-stimulus intervals (ISI = 100, 250, 400, and 1000 ms) to determine the relative time course of the different features. Notably, action priming effects were found in ISIs of 100, 250, and 1000 ms whereas a visual priming effect was seen only in the ISI of 1000 ms. Importantly, our data suggest that features follow different time courses of activation during word recognition. In this regard, feature activation is dynamic, measurable in specific time windows but not in others. Thus the current study (1) demonstrates how multiple ISIs can be used within an experiment to help chart the time course of feature activation and (2) provides new evidence for embodied theories of language. PMID:26074836

  5. Pro-oxidative action of polyphenols as action mechanism for their pro-apoptotic activity.

    PubMed

    Lecci, Raffaella Marina; Logrieco, Antonio; Leone, Antonella

    2014-01-01

    Polyphenols, secondary metabolites widely present in plant kingdom, are known for their positive effects on human health, such as treatments of degenerative disease and cancer. Many dietary polyphenols show anti-inflammatory, immunomodulatory and antioxidant properties and they are proposed as chemopreventive agents for many skin disorders and cancer. Exposure to solar UV radiation is widely considered to cause skin cancer and a consistent carcinogenic dose derived from UVA causes several skin disorders as a consequence of free radicals generation and DNA damages. In this study, verbascoside, isoverbascoside and tyrosol were investigated for their effects on HEKa (Human Epidermal Keratinocytes adult) cell cultures challenged from UVA-rays. Non-toxic doses of each polyphenol were assayed on HEKa before, during and after the exposure to a damaging dose of UVA. Treatment with polyphenols before and after the UVA-irradiation exerted a pro-oxidant effect, while the simultaneous treatment caused a weak decrease of ROS production. The increasing of ROS levels was associated with a proapoptotic effect on HEKa, detected by AnnexinV/Propidiun Iodide, mainly evident in surviving cells treated with the polyphenols after the UVA-irradiation. The pro-apoptotic effect was confirmed by the immunodetection of significant changes in the Bax and Bcl-xL protein levels, leading to apoptotic events. The hypothesis that these polyphenols could trigger the apoptosis pathway mainly in UVA-damaged cells, via ROS increase, is here proposed as action mechanism behind their protective effect.

  6. Action Research to Encourage Pupils' Active Participation in the Sustainable School

    ERIC Educational Resources Information Center

    Katsenou, Christina; Flogaitis, Evgenia; Liarakou, Georgia

    2015-01-01

    This article aims to explore the contribution of action research to the development of active participation of pupils in the context of the sustainable school. Action research is looked at not simply as a methodological tool for the exploration of participation, but as a key element of the educational actions that promote the active participation…

  7. Corrective Action Investigation Plan for Corrective Action Unit 127: Areas 25 and 26 Storage Tanks, Nevada Test Site, Nevada (Rev. No.: 0, August 2002)

    SciTech Connect

    NNSA /NV

    2002-08-27

    contaminated materials. Future land-use scenarios limit subsequent uses of the CASs to various nonresidential (i.e., industrial) activities. Field activities will consist of radiological walkover and screening surveys, and field-screening and collecting of both tank content and soil samples, and further sample testing as appropriate. A two-step data quality objective strategy will be followed: (1) Phase I will be to collect environmental samples for laboratory analysis to confirm the presence or absence of contaminants at concentrations exceeding preliminary action levels; and (2) Phase II will be to collect additional environmental samples for laboratory analysis to determine the extent of contamination identified in Phase I. The results of this field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  8. Processing of action- but not stimulus-related prediction errors differs between active and observational feedback learning.

    PubMed

    Kobza, Stefan; Bellebaum, Christian

    2015-01-01

    Learning of stimulus-response-outcome associations is driven by outcome prediction errors (PEs). Previous studies have shown larger PE-dependent activity in the striatum for learning from own as compared to observed actions and the following outcomes despite comparable learning rates. We hypothesised that this finding relates primarily to a stronger integration of action and outcome information in active learners. Using functional magnetic resonance imaging, we investigated brain activations related to action-dependent PEs, reflecting the deviation between action values and obtained outcomes, and action-independent PEs, reflecting the deviation between subjective values of response-preceding cues and obtained outcomes. To this end, 16 active and 15 observational learners engaged in a probabilistic learning card-guessing paradigm. On each trial, active learners saw one out of five cues and pressed either a left or right response button to receive feedback (monetary win or loss). Each observational learner observed exactly those cues, responses and outcomes of one active learner. Learning performance was assessed in active test trials without feedback and did not differ between groups. For both types of PEs, activations were found in the globus pallidus, putamen, cerebellum, and insula in active learners. However, only for action-dependent PEs, activations in these structures and the anterior cingulate were increased in active relative to observational learners. Thus, PE-related activity in the reward system is not generally enhanced in active relative to observational learning but only for action-dependent PEs. For the cerebellum, additional activations were found across groups for cue-related uncertainty, thereby emphasising the cerebellum's role in stimulus-outcome learning.

  9. An integrated approach for prospectively investigating a mode-of-action for rodent liver effects.

    PubMed

    LeBaron, Matthew J; Geter, David R; Rasoulpour, Reza J; Gollapudi, B Bhaskar; Thomas, Johnson; Murray, Jennifer; Kan, H Lynn; Wood, Amanda J; Elcombe, Cliff; Vardy, Audrey; McEwan, Jillian; Terry, Claire; Billington, Richard

    2013-07-15

    Registration of new plant protection products (e.g., herbicide, insecticide, or fungicide) requires comprehensive mammalian toxicity evaluation including carcinogenicity studies in two species. The outcome of the carcinogenicity testing has a significant bearing on the overall human health risk assessment of the substance and, consequently, approved uses for different crops across geographies. In order to understand the relevance of a specific tumor finding to human health, a systematic, transparent, and hypothesis-driven mode of action (MoA) investigation is, appropriately, an expectation by the regulatory agencies. Here, we describe a novel approach of prospectively generating the MoA data by implementing additional end points to the standard guideline toxicity studies with sulfoxaflor, a molecule in development. This proactive MoA approach results in a more robust integration of molecular with apical end points while minimizing animal use. Sulfoxaflor, a molecule targeting sap-feeding insects, induced liver effects (increased liver weight due to hepatocellular hypertrophy) in an initial palatability probe study for selecting doses for subsequent repeat-dose dietary studies. This finding triggered the inclusion of dose-response investigations of the potential key events for rodent liver carcinogenesis, concurrent with the hazard assessment studies. As predicted, sulfoxaflor induced liver tumors in rats and mice in the bioassays. The MoA data available by the time of the carcinogenicity finding supported the conclusion that the carcinogenic potential of sulfoxaflor was due to CAR/PXR nuclear receptor activation with subsequent hepatocellular proliferation. This MoA was not considered to be relevant to humans as sulfoxaflor is unlikely to induce hepatocellular proliferation in humans and therefore would not be a human liver carcinogen.

  10. Corrective Action Investigation Plan for Corrective Action Unit 568: Area 3 Plutonium Dispersion Sites Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Matthews, Patrick

    2014-01-01

    CAU 568 is a grouping of sites where there has been a suspected release of contamination associated with nuclear testing. This document describes the planned investigation of CAU 568, which comprises the following corrective action sites (CASs): • 03-23-17, S-3I Contamination Area • 03-23-19, T-3U Contamination Area • 03-23-20, Otero Contamination Area • 03-23-22, Platypus Contamination Area • 03-23-23, San Juan Contamination Area • 03-23-26, Shrew/Wolverine Contamination Area These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the investigation report.

  11. Microcalorimetric investigation of the toxic action of berberine on Tetrahymena thermophila BF(5).

    PubMed

    Kong, Weijun; Li, Zulun; Xiao, Xiaohe; Zhao, Yanling

    2010-10-01

    Tetrahymena thermophila (T. thermophila) BF(5) produces heat through growth and metabolism. By microcalorimetry, the power-time curves of the metabolism of T. thermophila BF(5) at 28 °C were measured and some quantitative parameters were obtained from these curves. Then the action of berberine on this microbe was investigated. Furthermore, the minimum inhibitory concentration (MIC) of berberine against T. thermophila BF(5) growth was obtained by tube dilution method. Berberine of different concentrations had various actions on T. thermophila BF(5) growth: a low concentration (25 μg/ml) of berberine began to inhibit the growth of T. thermophila BF(5) and a high concentration (450 μg/ml) of berberine completely inhibited T. thermophila BF(5) growth. The toxic action of berberine could also be expressed as half-inhibitory concentration IC(50), i.e., 50% effective in this inhibition. The value of IC(50) was 175.60 μg/ml, while the MIC of this compound against T. thermophila BF(5) was 20.76 mg/ml. Berberine has strong toxic action on T. thermophila BF(5) growth. The microcalorimetric method for the assay of toxic action is quantitative, inexpensive and versatile.

  12. Corrective Action Investigation Plan for Corrective Action Unit 516: Septic Systems and Discharge Points, Nevada Test Site, Nevada, Rev. 0, Including Record of Technical Change No. 1

    SciTech Connect

    2003-04-28

    This Corrective Action Investigation Plan (CAIP) contains the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Sites Office's (NNSA/NSO's) approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 516, Septic Systems and Discharge Points, Nevada Test Site (NTS), Nevada, under the Federal Facility Agreement and Consent Order. CAU 516 consists of six Corrective Action Sites: 03-59-01, Building 3C-36 Septic System; 03-59-02, Building 3C-45 Septic System; 06-51-01, Sump Piping, 06-51-02, Clay Pipe and Debris; 06-51-03, Clean Out Box and Piping; and 22-19-04, Vehicle Decontamination Area. Located in Areas 3, 6, and 22 of the NTS, CAU 516 is being investigated because disposed waste may be present without appropriate controls, and hazardous and/or radioactive constituents may be present or migrating at concentrations and locations that could potentially pose a threat to human health and the environment. Existing information and process knowledge on the expected nature and extent of contamination of CAU 516 are insufficient to select preferred corrective action alternatives; therefore, additional information will be obtained by conducting a corrective action investigation. The results of this field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document. Record of Technical Change No. 1 is dated 3/2004.

  13. Corrective action investigation plan for Central Nevada Test Area, CAU No. 417

    SciTech Connect

    1997-04-01

    This Corrective Action Investigation Plan (CAIP) is part of a US Department of Energy (DOE)-funded environmental investigation of the Central Nevada Test Area (CNTA). This CAIP addresses the surface investigation and characterization of 15 identified Corrective Action Sites (CASs). In addition, several other areas of the CNTA project area have surface expressions that may warrant investigation. These suspect areas will be characterized, if necessary, in subsequent CAIPs or addendums to this CAIP prepared to address these sites. This CAIP addresses only the 15 identified CASs as shown in Table 2-1 that are associated with the drilling and construction of a number of testing wells designed as part of an underground nuclear testing program. The purpose of the wells at the time of construction was to provide subsurface access for the emplacement, testing, and post detonation evaluations of underground nuclear devices. If contamination is found at any of the 15-surface CASs, the extent of contamination will be determined in order to develop an appropriate corrective action.

  14. Herbicide activity of monosulfuron and its mode of action.

    PubMed

    Fan, Zhi-Jin; Ai, Ying-Wei; Qian, Chuan-Fan; Li, Zheng-Ming

    2005-01-01

    Monosulfuron was developed for weed control in the field of wheat (Triticum, aestivum L.) and millet (Panicum miliaceum L.) with the application rate ranging from 15 to 60 g ai/hm2. Herbicidal activity of monosulfuron was evaluated systematically by bioassay using maize (Zea mays L.) taproot as indicator and weed fresh weight of Acalypha australis L. and Echinochloa phyllopogon. Maize CAU 3138 was the most tolerant cultivars to monosulfuron with IC50 (concentration of 50% inhibition) of 85 microg/kg, Yedan 13 was one of the most sensitive cultivars to monosulfuron with IC50 of 6.4 microg/kg. Monosulfuron inhibited the growth of Acalypha australis L. strongly comparing with that of Echinochloa phyllopogon. Monosulfuron was a good acetolactate synthase (ALS) inhibitor in vitro, the I50 (50% of inhibition) of monosulfuron, chlorsulfuron, tribenuron-methyl and nicosulfuron for CAU 3138 were 32, 2, 19 and 26 nmol/L respectively, for Yedan 13 the I50 were 15, 3, 17 and 65 nmol/L respectively. In vivo ALS inhibition occurred only in higher concentration of 4 sulfonylurea herbicide tested. Comparison study of this test indicated that the mode of action of monosulfuron was the same as that of other sulfonylurea herbicides such as chlorsulfuron, tribenuron-methyl and nicosulfuron, they were all inhibitors targeted at the ALS. PMID:16083111

  15. Modes of action of three disinfectant active substances: a review.

    PubMed

    Wessels, Stephen; Ingmer, Hanne

    2013-12-01

    This review deals with three categories of active substances for disinfectant products, their modes of action (MOA), and how MOA can help predict propensity for resistance in microorganisms. Within the European Union applications for approval of disinfectants of all kinds must be submitted in a few years, and documentation on MOA and resistance must be part of those applications. Peracetic acid is an unspecific, pervasive oxidizer of C-C double bonds and reduced atoms. This MOA would imply poor chance for development of resistance in microorganisms, as borne out by the absence of such reports in the literature. The quaternary ammonium compounds (QAC's) are much more specific in their antimicrobial mechanism. Even very low concentrations cause damage to the cytoplasmic membrane due to perturbation of the bilayers by the molecules' alkyl chains. Development of microbial resistance to QAC's, as well as cross-resistance to antibiotics, are particularly well documented. The polymer PHMB is antimicrobial because it disturbs the cell membrane's bilayer by interacting with it along the surface of the membrane. Resistance to the polymer appears not to develop despite many years of use in many fields. However, PHMB's toxicity to humans upon inhalation dictates great caution when deploying the substance.

  16. Students of Action? A Comparative Investigation of Secondary Science and Social Studies Students' Action Repertoires in a Land Use Context

    ERIC Educational Resources Information Center

    Kumler, Lori M.

    2011-01-01

    Environmental education (EE) and social studies education share an interest in behavioral outcomes. This study compares behavioral outcomes--including both self-reported knowledge of actions and reported actions taken--in the context of a land use curriculum enacted in secondary science versus social studies classes with 500 students and nine…

  17. Prediction of human actions: expertise and task-related effects on neural activation of the action observation network.

    PubMed

    Balser, Nils; Lorey, Britta; Pilgramm, Sebastian; Stark, Rudolf; Bischoff, Matthias; Zentgraf, Karen; Williams, Andrew Mark; Munzert, Jörn

    2014-08-01

    The action observation network (AON) is supposed to play a crucial role when athletes anticipate the effect of others' actions in sports such as tennis. We used functional magnetic resonance imaging to explore whether motor expertise leads to a differential activation pattern within the AON during effect anticipation and whether spatial and motor anticipation tasks are associated with a differential activation pattern within the AON depending on participant expertise level. Expert (N=16) and novice (N=16) tennis players observed video clips depicting forehand strokes with the instruction to either indicate the predicted direction of ball flight (spatial anticipation) or to decide on an appropriate response to the observed action (motor anticipation). The experts performed better than novices on both tennis anticipation tasks, with the experts showing stronger neural activation in areas of the AON, namely, the superior parietal lobe, the intraparietal sulcus, the inferior frontal gyrus, and the cerebellum. When novices were contrasted with experts, motor anticipation resulted in stronger activation of the ventral premotor cortex, the supplementary motor area, and the superior parietal lobe than spatial anticipation task did. In experts, the comparison of motor and spatial anticipation revealed no increased activation. We suggest that the stronger activation of areas in the AON during the anticipation of action effects in experts reflects their use of the more fine-tuned motor representations they have acquired and improved during years of training. Furthermore, results suggest that the neural processing of different anticipation tasks depends on the expertise level.

  18. Geometric investigation of a gaming active device

    NASA Astrophysics Data System (ADS)

    Menna, Fabio; Remondino, Fabio; Battisti, Roberto; Nocerino, Erica

    2011-07-01

    3D imaging systems are widely available and used for surveying, modeling and entertainment applications, but clear statements regarding their characteristics, performances and limitations are still missing. The VDI/VDE and the ASTME57 committees are trying to set some standards but the commercial market is not reacting properly. Since many new users are approaching these 3D recording methodologies, clear statements and information clarifying if a package or system satisfies certain requirements before investing are fundamental for those users who are not really familiar with these technologies. Recently small and portable consumer-grade active sensors came on the market, like TOF rangeimaging cameras or low-cost triangulation-based range sensor. A quite interesting active system was produced by PrimeSense and launched on the market thanks to the Microsoft Xbox project with the name of Kinect. The article reports the geometric investigation of the Kinect active sensors, considering its measurement performances, the accuracy of the retrieved range data and the possibility to use it for 3D modeling application.

  19. Corrective Action Investigation Plan for Corrective Action Unit 97: Yucca Flat/Climax Mine, Nevada National Security Site, Nevada with ROTCs 1, 2, and 3 (Revision 0, September 2000)

    SciTech Connect

    Andrews, Robert; Marutzky, Sam

    2000-09-01

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operations Office's (DOE/NV's) approach to collect the data necessary to evaluate Corrective Action Alternatives (CAAs) appropriate for the closure of Corrective Action Unit (CAU) 97 under the Federal Facility Agreement and Consent Order (FFACO). Corrective Action Unit 97, collectively known as the Yucca Flat/Climax Mine CAU, consists of 720 Corrective Action Sites (CASs). The Yucca Flat/Climax Mine CAU extends over several areas of the NTS and constitutes one of several areas used for underground nuclear testing in the past. The nuclear tests resulted in groundwater contamination in the vicinity as well as downgradient of the underground test areas. Based on site history, the Yucca Flat underground nuclear tests were conducted in alluvial, volcanic, and carbonate rocks; whereas, the Climax Mine tests were conducted in an igneous intrusion located in northern Yucca Flat. Particle-tracking simulations performed during the regional evaluation indicate that the local Climax Mine groundwater flow system merges into the much larger Yucca Flat groundwater flow systems during the 1,000-year time period of interest. Addressing these two areas jointly and simultaneously investigating them as a combined CAU has been determined the best way to proceed with corrective action investigation (CAI) activities. The purpose and scope of the CAI includes characterization activities and model development conducted in five major sequential steps designed to be consistent with FFACO Underground Test Area Project's strategy to predict the location of the contaminant boundary, develop and implement a corrective action, and close each CAU. The results of this field investigation will support a defensible evaluation of CAAs in the subsequent corrective action decision document.

  20. Cortical alpha activity predicts the confidence in an impending action

    PubMed Central

    Kubanek, Jan; Hill, N. Jeremy; Snyder, Lawrence H.; Schalk, Gerwin

    2015-01-01

    When we make a decision, we experience a degree of confidence that our choice may lead to a desirable outcome. Recent studies in animals have probed the subjective aspects of the choice confidence using confidence-reporting tasks. These studies showed that estimates of the choice confidence substantially modulate neural activity in multiple regions of the brain. Building on these findings, we investigated the neural representation of the confidence in a choice in humans who explicitly reported the confidence in their choice. Subjects performed a perceptual decision task in which they decided between choosing a button press or a saccade while we recorded EEG activity. Following each choice, subjects indicated whether they were sure or unsure about the choice. We found that alpha activity strongly encodes a subject's confidence level in a forthcoming button press choice. The neural effect of the subjects' confidence was independent of the reaction time and independent of the sensory input modeled as a decision variable. Furthermore, the effect is not due to a general cognitive state, such as reward expectation, because the effect was specifically observed during button press choices and not during saccade choices. The neural effect of the confidence in the ensuing button press choice was strong enough that we could predict, from independent single trial neural signals, whether a subject was going to be sure or unsure of an ensuing button press choice. In sum, alpha activity in human cortex provides a window into the commitment to make a hand movement. PMID:26283892

  1. 34 CFR 426.7 - What activities does the Secretary fund under the Agriculture Action Centers?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Agriculture Action Centers? 426.7 Section 426.7 Education Regulations of the Offices of the Department of... DEMONSTRATION PROGRAM General § 426.7 What activities does the Secretary fund under the Agriculture Action Centers? The Secretary supports model Agriculture Action Centers that provide improved access...

  2. Making Tracks 1.0: Action Researching an Active Transportation Education Program

    ERIC Educational Resources Information Center

    Robinson, Daniel; Foran, Andrew; Robinson, Ingrid

    2014-01-01

    This paper reports on the results of the first cycle of an action research project. The objective of this action research was to examine the implementation of a school-based active transportation education program (Making Tracks). A two-cycle action research design was employed in which elementary school students' (ages 7-9), middle school…

  3. 34 CFR 426.7 - What activities does the Secretary fund under the Agriculture Action Centers?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Agriculture Action Centers? 426.7 Section 426.7 Education Regulations of the Offices of the Department of... DEMONSTRATION PROGRAM General § 426.7 What activities does the Secretary fund under the Agriculture Action Centers? The Secretary supports model Agriculture Action Centers that provide improved access...

  4. 34 CFR 426.7 - What activities does the Secretary fund under the Agriculture Action Centers?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Agriculture Action Centers? 426.7 Section 426.7 Education Regulations of the Offices of the Department of... DEMONSTRATION PROGRAM General § 426.7 What activities does the Secretary fund under the Agriculture Action Centers? The Secretary supports model Agriculture Action Centers that provide improved access...

  5. Cycles of Action through Systems of Activity: Examining an Action Research Model through the Lens of Activity Theory

    ERIC Educational Resources Information Center

    Orland-Barak, Lily; Becher, Ayelet

    2011-01-01

    In this article we offer an extended reading of an action research model in the context of mentored learning in preservice education in Israel. Our reading attends both to how a particular form of action research plays out in participants' constructions of the practice of mentoring and mentored learning and how such constructions can be understood…

  6. Action sentences activate sensory motor regions in the brain independently of their status of reality.

    PubMed

    de Vega, Manuel; León, Inmaculada; Hernández, Juan A; Valdés, Mitchell; Padrón, Iván; Ferstl, Evelyn C

    2014-07-01

    Some studies have reported that understanding concrete action-related words and sentences elicits activations of motor areas in the brain. The present fMRI study goes one step further by testing whether this is also the case for comprehension of nonfactual statements. Three linguistic structures were used (factuals, counterfactuals, and negations), referring either to actions or, as a control condition, to visual events. The results showed that action sentences elicited stronger activations than visual sentences in the SMA, extending to the primary motor area, as well as in regions generally associated with the planning and understanding of actions (left superior temporal gyrus, left and right supramarginal gyri). Also, we found stronger activations for action sentences than for visual sentences in the extrastriate body area, a region involved in the visual processing of human body movements. These action-related effects occurred not only in factuals but also in negations and counterfactuals, suggesting that brain regions involved in action understanding and planning are activated by default even when the actions are described as hypothetical or as not happening. Moreover, some of these regions overlapped with those activated during the observation of action videos, indicating that the act of understanding action language and that of observing real actions share neural networks. These results support the claim that embodied representations of linguistic meaning are important even in abstract linguistic contexts.

  7. An integrated approach for prospectively investigating a mode-of-action for rodent liver effects

    SciTech Connect

    LeBaron, Matthew J.; Geter, David R.; Rasoulpour, Reza J.; Gollapudi, B. Bhaskar; Thomas, Johnson; Murray, Jennifer; Kan, H. Lynn; Wood, Amanda J.; Elcombe, Cliff; Vardy, Audrey; McEwan, Jillian; Terry, Claire; Billington, Richard

    2013-07-15

    Registration of new plant protection products (e.g., herbicide, insecticide, or fungicide) requires comprehensive mammalian toxicity evaluation including carcinogenicity studies in two species. The outcome of the carcinogenicity testing has a significant bearing on the overall human health risk assessment of the substance and, consequently, approved uses for different crops across geographies. In order to understand the relevance of a specific tumor finding to human health, a systematic, transparent, and hypothesis-driven mode of action (MoA) investigation is, appropriately, an expectation by the regulatory agencies. Here, we describe a novel approach of prospectively generating the MoA data by implementing additional end points to the standard guideline toxicity studies with sulfoxaflor, a molecule in development. This proactive MoA approach results in a more robust integration of molecular with apical end points while minimizing animal use. Sulfoxaflor, a molecule targeting sap-feeding insects, induced liver effects (increased liver weight due to hepatocellular hypertrophy) in an initial palatability probe study for selecting doses for subsequent repeat-dose dietary studies. This finding triggered the inclusion of dose-response investigations of the potential key events for rodent liver carcinogenesis, concurrent with the hazard assessment studies. As predicted, sulfoxaflor induced liver tumors in rats and mice in the bioassays. The MoA data available by the time of the carcinogenicity finding supported the conclusion that the carcinogenic potential of sulfoxaflor was due to CAR/PXR nuclear receptor activation with subsequent hepatocellular proliferation. This MoA was not considered to be relevant to humans as sulfoxaflor is unlikely to induce hepatocellular proliferation in humans and therefore would not be a human liver carcinogen. - Highlights: • We prospectively generated MoA data into standard guideline toxicity studies. • A proactive MoA approach

  8. An investigation into the antimicrobial mechanisms of action of two contact lens biocides using electron microscopy.

    PubMed

    Codling, Caroline E; Hann, Anthony C; Maillard, Jean-Yves; Russell, A Denver

    2005-12-01

    Polyquaternium-1 (PQ-1) and myristamidopropyl dimethylamine (MAPD) are biocides used commercially in a contact lens disinfecting solutions. Electron microscopy was used to provide further evidence on the mechanism(s) of action of these agents against a wide range of ocular pathogens including bacteria, fungi and protozoa. Both PQ-1 and MAPD caused multiple forms of damage to the organisms tested, evidenced by structural alterations, blebbing, leakage and cell destruction. The extent of damage and the selectivity against specific type of microorganisms was consistent with the antimicrobial activity of these agents. Although electron microscopy is a powerful tool, it has its limitations when used to examine the mode of action of biocides. Indeed, there was no evidence of gross structural alteration to Acanthamoeba castellani or Aspergillus fumigatus following treatment.

  9. An investigation into the antimicrobial mechanisms of action of two contact lens biocides using electron microscopy.

    PubMed

    Codling, Caroline E; Hann, Anthony C; Maillard, Jean-Yves; Russell, A Denver

    2005-12-01

    Polyquaternium-1 (PQ-1) and myristamidopropyl dimethylamine (MAPD) are biocides used commercially in a contact lens disinfecting solutions. Electron microscopy was used to provide further evidence on the mechanism(s) of action of these agents against a wide range of ocular pathogens including bacteria, fungi and protozoa. Both PQ-1 and MAPD caused multiple forms of damage to the organisms tested, evidenced by structural alterations, blebbing, leakage and cell destruction. The extent of damage and the selectivity against specific type of microorganisms was consistent with the antimicrobial activity of these agents. Although electron microscopy is a powerful tool, it has its limitations when used to examine the mode of action of biocides. Indeed, there was no evidence of gross structural alteration to Acanthamoeba castellani or Aspergillus fumigatus following treatment. PMID:16332500

  10. Do displacement activities help preschool children to inhibit a forbidden action?

    PubMed

    Pecora, Giulia; Addessi, Elsa; Schino, Gabriele; Bellagamba, Francesca

    2014-10-01

    Displacement activities are commonly recognized as behavioral patterns, mostly including self-directed actions (e.g., scratching, self-touching), that often occur in situations involving conflicting motivational tendencies. In ethology, several researchers have suggested that displacement activities could facilitate individuals in dealing with the stress experienced in a frustrating context. In child developmental research, some authors have assessed whether distraction strategies could help children to inhibit a dominant response during delay of gratification tasks. However, little is known about the displacement activities that young children may produce in such situations. This study was aimed at investigating whether displacement activities had an effect on preschool children's ability to postpone an immediate gratification (i.e., interacting with an attractive toy, a musical box), thereby functioning as regulators of their emotional state. To this end, we administered 143 2- to 4-year-olds with a delay maintenance task and related their performance with displacement activities they produced during the task and with actions with an external object. Children's latency to touch the musical box was positively related to their rate of displacement activities. However, the rate of displacement activities increased progressively as long as the children were able to inhibit the interaction with the musical box. In addition, the rate of displacement activities during the first 1 min of test did not predict the ability of children to inhibit the interaction with the box. These results suggest that displacement activities represented a functionless by-product of motivational conflict rather than a strategy that children used to inhibit their response to an attractive stimulus. PMID:24907630

  11. Duration of activity and mode of action of modafinil: Studies on sleep and wakefulness in humans.

    PubMed

    Turner, C; Belyavin, A J; Nicholson, A N

    2014-07-01

    The duration of activity of modafinil was investigated in healthy male volunteers in two double-blind crossover studies. Mode of action was explored using a statistical model concerned with the relationship between total sleep duration and that of rapid eye movement (REM) sleep. Nocturnal sleep (23:00-07:00) followed by next-day performance (09:00-17:00) was studied in 12 subjects administered 100, 200, 300 mg modafinil and placebo, 0.5 h before bedtime. Performance overnight (19:00-08:45) followed by sleep (09:15-15:15) was studied in nine subjects administered 100, 200, 300, 400 mg modafinil, 300 mg caffeine and placebo at 22:15. Modafinil dose-dependently reduced sleep duration (nocturnal: 200 mg, p<0.05; 300 mg, p<0.001; morning: 300 and 400 mg, p<0.05) and REM sleep (nocturnal: 300 mg; morning: 400 mg; p<0.05). The statistical model revealed that reduced REM sleep was due to alerting activity, with no evidence of direct suppression of REM sleep, suggesting dopaminergic activity. Enhanced performance with modafinil during overnight work varied with dose (200 mg>100 mg; 300, 400 mg>200, 100 mg, caffeine). However, in the study of next-day performance, the enhancement was attenuated at the highest dose (300 mg) by the greater disturbance of prior sleep. These findings indicate that modafinil has a long duration of action, with alerting properties arising predominantly from dopaminergic activity. PMID:24306135

  12. Action preparation modulates sensory perception in unseen personal space: An electrophysiological investigation.

    PubMed

    Job, Xavier E; de Fockert, Jan W; van Velzen, José

    2016-08-01

    Behavioural and electrophysiological evidence has demonstrated that preparation of goal-directed actions modulates sensory perception at the goal location before the action is executed. However, previous studies have focused on sensory perception in areas of peripersonal space. The present study investigated visual and tactile sensory processing at the goal location of upcoming movements towards the body, much of which is not visible, as well as visible peripersonal space. A motor task cued participants to prepare a reaching movement towards goals either in peripersonal space in front of them or personal space on the upper chest. In order to assess modulations of sensory perception during movement preparation, event-related potentials (ERPs) were recorded in response to task-irrelevant visual and tactile probe stimuli delivered randomly at one of the goal locations of the movements. In line with previous neurophysiological findings, movement preparation modulated visual processing at the goal of a movement in peripersonal space. Movement preparation also modulated somatosensory processing at the movement goal in personal space. The findings demonstrate that tactile perception in personal space is subject to similar top-down sensory modulation by motor preparation as observed for visual stimuli presented in peripersonal space. These findings show for the first time that the principles and mechanisms underlying adaptive modulation of sensory processing in the context of action extend to tactile perception in unseen personal space.

  13. WSSRAP chemical plant geotechnical investigations for the Weldon Spring Site Remedial Action Project, Weldon Spring, Missouri

    SciTech Connect

    Not Available

    1990-12-01

    This document has been prepared for the United states Department of Energy (DOE) Weldon Spring Site Remedial Action Project (WSSRAP) by the Project Management Contractor (PMC), which consists of MK-Ferguson Company (MKF) and Morrison Knudsen Corporation Environmental Services Group (MKES) with Jacobs Engineering Group (JEG) as MKF's predesignated subcontractor. This report presents the results of site geotechnical investigations conducted by the PMC in the vicinity of the Weldon Spring chemical plant and raffinate pits (WSCP/RP) and in potential on-site and off-site clayey material borrow sources. The WSCP/RP is the proposed disposal cell (DC) site. 39 refs., 24 figs., 12 tabs.

  14. By the sound of it. An ERP investigation of human action sound processing in 7-month-old infants

    PubMed Central

    Geangu, Elena; Quadrelli, Ermanno; Lewis, James W.; Macchi Cassia, Viola; Turati, Chiara

    2015-01-01

    Recent evidence suggests that human adults perceive human action sounds as a distinct category from human vocalizations, environmental, and mechanical sounds, activating different neural networks (Engel et al., 2009; Lewis et al., 2011). Yet, little is known about the development of such specialization. Using event-related potentials (ERP), this study investigated neural correlates of 7-month-olds’ processing of human action (HA) sounds in comparison to human vocalizations (HV), environmental (ENV), and mechanical (MEC) sounds. Relative to the other categories, HA sounds led to increased positive amplitudes between 470 and 570 ms post-stimulus onset at left anterior temporal locations, while HV led to increased negative amplitudes at the more posterior temporal locations in both hemispheres. Collectively, human produced sounds (HA + HV) led to significantly different response profiles compared to non-living sound sources (ENV + MEC) at parietal and frontal locations in both hemispheres. Overall, by 7 months of age human action sounds are being differentially processed in the brain, consistent with a dichotomy for processing living versus non-living things. This provides novel evidence regarding the typical categorical processing of socially relevant sounds. PMID:25732377

  15. Corrective Action Investigation Plan for Corrective Action Unit 99: Rainier Mesa/Shoshone Mountain, Nevada Test Site, Nevada with Errata and ROTC 1, Rev. No. 0

    SciTech Connect

    McCord, John; Marutzky, Sam

    2004-12-01

    This Corrective Action Investigation Plan (CAIP) was developed for Corrective Action Unit (CAU) 99, Rainier Mesa/Shoshone Mountain. The CAIP is a requirement of the ''Federal Facility Agreement and Consent Order'' (FFACO) agreed to by the State of Nevada, the U.S. Department of Energy (DOE), and the U.S. Department of Defense (DoD) (FFACO, 1996). The FFACO addresses environmental restoration activities at U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) facilities and sites including the underground testing area(s) of the Nevada Test Site (NTS). This CAIP describes the investigation activities currently planned for the Rainier Mesa/Shoshone Mountain CAU. These activities are consistent with the current Underground Test Area (UGTA) Project strategy described in Section 3.0 of Appendix VI, Revision No. 1 (December 7, 2000) of the FFACO (1996) and summarized in Section 2.1.2 of this plan. The Rainier Mesa/Shoshone Mountain CAU extends over several areas of the NTS (Figure 1-1) and includes former underground nuclear testing locations in Areas 12 and 16. The area referred to as ''Rainier Mesa'' includes the geographical area of Rainier Mesa proper and the contiguous Aqueduct Mesa. Figure 1-2 shows the locations of the tests (within tunnel complexes) conducted at Rainier Mesa. Shoshone Mountain is located approximately 20 kilometers (km) south of Rainier Mesa, but is included within the same CAU due to similarities in their geologic setting and in the nature and types of nuclear tests conducted. Figure 1-3 shows the locations of the tests conducted at Shoshone Mountain. The Rainier Mesa/Shoshone Mountain CAU falls within the larger-scale Rainier Mesa/Shoshone Mountain Investigation Area, which also includes the northwest section of the Yucca Flat CAU as shown in Figure 1-1. Rainier Mesa and Shoshone Mountain lie adjacent to the Timber Mountain Caldera Complex and are composed of volcanic rocks that erupted from the

  16. Comparative investigations of manual action representations: evidence that chimpanzees represent the costs of potential future actions involving tools

    PubMed Central

    Frey, Scott H.; Povinelli, Daniel J.

    2012-01-01

    The ability to adjust one's ongoing actions in the anticipation of forthcoming task demands is considered as strong evidence for the existence of internal action representations. Studies of action selection in tool use reveal that the behaviours that we choose in the present moment differ depending on what we intend to do next. Further, they point to a specialized role for mechanisms within the human cerebellum and dominant left cerebral hemisphere in representing the likely sensory costs of intended future actions. Recently, the question of whether similar mechanisms exist in other primates has received growing, but still limited, attention. Here, we present data that bear on this issue from a species that is a natural user of tools, our nearest living relative, the chimpanzee. In experiment 1, a subset of chimpanzees showed a non-significant tendency for their grip preferences to be affected by anticipation of the demands associated with bringing a tool's baited end to their mouths. In experiment 2, chimpanzees' initial grip preferences were consistently affected by anticipation of the forthcoming movements in a task that involves using a tool to extract a food reward. The partial discrepancy between the results of these two studies is attributed to the ability to accurately represent differences between the motor costs associated with executing the two response alternatives available within each task. These findings suggest that chimpanzees are capable of accurately representing the costs of intended future actions, and using those predictions to select movements in the present even in the context of externally directed tool use. PMID:22106426

  17. Corrective Action Investigation Plan for Corrective Action Unit 254: Area 25 R-MAD Decontamination Facility, Nevada Test Site, Nevada (includes ROTC No. 1, date 01/25/1999)

    SciTech Connect

    DOE /NV

    1999-07-29

    This Corrective Action Investigation Plan contains the US Department of Energy, Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 254 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 254 consists of Corrective Action Site (CAS) 25-23-06, Decontamination Facility. Located in Area 25 at the Nevada Test Site (NTS), CAU 254 was used between 1963 through 1973 for the decontamination of test-car hardware and tooling used in the Nuclear Rocket Development Station program. The CAS is composed of a fenced area measuring approximately 119 feet by 158 feet that includes Building 3126, an associated aboveground storage tank, a potential underground storage area, two concrete decontamination pads, a generator, two sumps, and a storage yard. Based on site history, the scope of this plan is to resolve the problem statement identified during the Data Quality Objectives process that decontamination activities at this CAU site may have resulted in the release of contaminants of concern (COCs) onto building surfaces, down building drains to associated leachfields, and to soils associated with two concrete decontamination pads located outside the building. Therefore, the scope of the corrective action field investigation will involve soil sampling at biased and random locations in the yard using a direct-push method, scanning and static radiological surveys, and laboratory analyses of all soil/building samples. Historical information provided by former NTS employees indicates that solvents and degreasers may have been used in the decontamination processes; therefore, potential COCs include volatile/semivolatile organic compounds, Resource Conservation and Recovery Act metals, petroleum hydrocarbons, polychlorinated biphenyls, pesticides, asbestos, gamma-emitting radionuclides, plutonium, uranium, and strontium-90. The results of this

  18. Anti-inflammatory activities of cecropin A and its mechanism of action.

    PubMed

    Lee, Eunjung; Shin, Areum; Kim, Yangmee

    2015-01-01

    Cecropin A is a novel 37-residue cecropin-like antimicrobial peptide isolated from the cecropia moth, Hyalophora cecropia. We have demonstrated that cecropin A is an antibacterial agent and have investigated its mode of action. In this study, we show that cecropin A has potent antimicrobial activity against 2 multidrug resistant organisms-Acinetobacter baumanii and-Pseudomonas aeruginosa. Interactions between cecropin A and membrane phospholipids were studied using tryptophan blue shift experiments. Cecropin A has a strong interaction with bacterial cell mimetic membranes. These results imply that cecropin A has selectivity for bacterial cells. To address the potential the rapeutic efficacy of cecropin A, its anti-inflammatory activities and mode of action in mouse macrophage-derived RAW264.7 cells stimulated with lipopolysaccharide (LPS) were examined. Cecropin A suppressed nitrite production, mTNF-α, mIL-1β, mMIP-1, and mMIP-2 cytokine release in LPS-stimulated RAW264.7 cells. Furthermore, cecropin A inhibited intracellular cell signaling via the ERK, JNK, and p38 MAPK pathway, leading to the prevention of COX-2 expression in LPS-stimulated RAW264.7 cells. These results strongly suggest that cecropin A should be investigated as a potential agent for the prevention and treatment of inflammatory diseases.

  19. Scientific rigour and innovations in participatory action research investigating workplace learning in continuing interprofessional education.

    PubMed

    Langlois, Sophie; Goudreau, Johanne; Lalonde, Lyne

    2014-05-01

    The persistent theory-practice gap shows how challenging it can be for healthcare professionals to keep updating their practices. The continuing education challenges are partly explained by the tremendous stream of new discoveries in health and the epidemic of multi-morbid conditions. Participatory action research (PAR) is used in healthcare as a research approach that capitalizes on people's resources to better understand and enhance their professional practices. PAR thus can consolidate our knowledge on workplace learning in continuing interprofessional education while directly improving quality of care. However, PAR lacks clear scientific criteria to ensure the consistency between the investigators' methodology and philosophy, which jeopardize its credibility. This paper outlines the principles of rigour in PAR and describes the additions of a preliminary planning phase to Kemmis and McTaggart's PAR description as well as the use of the professional co-development group, an action-oriented data collection method. We believe that this will help PAR co-participants achieve improved scientific rigour and encourage more investigators to collaborate through this research approach contributing to the advancement of knowledge on workplace learning in continuing interprofessional education. PMID:24559150

  20. Addendum to Revision 1 of the Corrective Action Investigation Plan for Corrective Action Unit 98: Frenchman Flat, Nevada Test Site, Nevada (Addendum Revision No. 1)

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office

    2001-06-06

    This document is submitted as an addendum to the Corrective Action Investigation Plan (CAIP) for Corrective Action Unit (CAU) 98: Frenchman Flat, Nevada Test Site (NTS), Nevada. The addendum was prepared to propose work activities in response to comments resulting from the U.S. Department of Energy's (DOE's) review of the draft Frenchman Flat CAU model of groundwater flow and contaminant transport completed in April 1999. The reviewers included an external panel of experts and the Nevada Division of Environmental Protection. As a result of the review, additional work scope, including new data-collection and modeling activities, has been identified for the Frenchman Flat CAU. The proposed work scope described in this addendum will be conducted in accordance with the revised Underground Test Area strategy contained in the December 2000 amendment to the Federal Facility Agreement and Consent Order. The Frenchman Flat CAU model is a group of interdependent models designed to predict the extent of contamination in groundwater due to the underground nuclear tests conducted within this CAU. At the time of the DOE review, the CAU model consisted of a CAU groundwater flow and transport model comprised of two major components: a groundwater flow model and a recharge model. The CAU groundwater flow model is supported by a hydrostratigraphic model and a recharge model, whereas the CAU transport model is supported by a source-term model. As part of the modeling activities proposed in this addendum, two new major components may be added to the Frenchman Flat CAU model: a total-system model and two local groundwater flow and transport models. The reviewers identified several issues relating to insufficiency of data and inadequacy of the modeling process that should be addressed to provide additional confidence in the modeling results with respect to the potential for contaminant migration to the Lower Carbonate Aquifer. The proposed additional work scope includes new data

  1. Corrective action investigation plan for Central Nevada Test Area CAU No. 417

    SciTech Connect

    1997-01-01

    This Corrective Action Investigation Plan (CAIP) is part of a US Department of Energy (DOE)-funded environmental investigation of the Central Nevada Test Area (CNTA). The CNTA is located in Hot Creek Valley in Nye County, Nevada, adjacent to US Highway 6, about 15 kilometers (10 miles) northeast of Warm Springs. The CNTA was the site of Project Faultless, a nuclear device detonated in the subsurface by the US Atomic Energy Commission (AEC) in January 1968. The purpose of this test was to gauge the seismic effects of relatively large, high-yield detonations completed outside of the Nevada Test Site (NTS). The test was also used to determine the suitability of the site for future large detonations. The yield of the Faultless test was between 200 kilotons and 1 megaton (DOE, 1994c).

  2. American Thyroid Association Guide to Investigating Thyroid Hormone Economy and Action in Rodent and Cell Models

    PubMed Central

    Anderson, Grant; Forrest, Douglas; Galton, Valerie Anne; Gereben, Balázs; Kim, Brian W.; Kopp, Peter A.; Liao, Xiao Hui; Obregon, Maria Jesus; Peeters, Robin P.; Refetoff, Samuel; Sharlin, David S.; Simonides, Warner S.; Weiss, Roy E.; Williams, Graham R.

    2014-01-01

    Background: An in-depth understanding of the fundamental principles that regulate thyroid hormone homeostasis is critical for the development of new diagnostic and treatment approaches for patients with thyroid disease. Summary: Important clinical practices in use today for the treatment of patients with hypothyroidism, hyperthyroidism, or thyroid cancer are the result of laboratory discoveries made by scientists investigating the most basic aspects of thyroid structure and molecular biology. In this document, a panel of experts commissioned by the American Thyroid Association makes a series of recommendations related to the study of thyroid hormone economy and action. These recommendations are intended to promote standardization of study design, which should in turn increase the comparability and reproducibility of experimental findings. Conclusions: It is expected that adherence to these recommendations by investigators in the field will facilitate progress towards a better understanding of the thyroid gland and thyroid hormone dependent processes. PMID:24001133

  3. Reputation in an economic game modulates premotor cortex activity during action observation.

    PubMed

    Farmer, Harry; Apps, Matthew; Tsakiris, Manos

    2016-09-01

    Our interactions with other people - and our processing of their actions - are shaped by their reputation. Research has identified an Action Observation Network (AON) which is engaged when observing other people's actions. Yet, little is known about how the processing of others' actions is influenced by another's reputation. Is the response of the AON modulated by the reputation of the actor? We developed a variant of the ultimatum game in which participants watched either the visible or occluded actions of two 'proposers'. These actions were tied to decisions of how to split a pot of money although the proposers' decisions on each trial were not known to participants when observing the actions. One proposer made fair offers on the majority of trials, establishing a positive reputation, whereas the other made predominantly, unfair offers resulting in a negative reputation. We found significant activations in two regions of the left dorsal premotor cortex (dPMC). The first of these showed a main effect of reputation with greater activation for the negative reputation proposer than the positive reputation proposer. Furthermore individual differences in trust ratings of the two proposers covaried with activation in the right primary motor cortex (M1). The second showed an interaction between visibility and reputation driven by a greater effect of reputation when participants were observing an occluded action. Our findings show that the processing of others' actions in the AON is modulated by an actor's reputation, and suggest a predictive role for the PMC during action observation. PMID:27364606

  4. Is Brain Activity during Action Observation Modulated by the Perceived Fairness of the Actor?

    PubMed

    Etzel, Joset A; Valchev, Nikola; Gazzola, Valeria; Keysers, Christian

    2016-01-01

    Perceiving other people's actions triggers activity in premotor and parietal areas, brain areas also involved in executing and sensing our own actions. Paralleling this phenomenon, observing emotional states (including pain) in others is associated with activity in the same brain areas as activated when experiencing similar emotions directly. This emotion perception associated activity has been shown to be affected by the perceived fairness of the actor, and in-group membership more generally. Here, we examine whether action observation associated brain activity is also affected by the perceived social fairness of the actors. Perceived fairness was manipulated using an alternating iterated Prisoner's Dilemma game between the participant and two confederates, one of whom played fairly and the other unfairly. During fMRI scanning the participants watched movies of the confederates performing object-directed hand actions, and then performed hand actions themselves. Mass-univariate analysis showed that observing the actions triggered robust activation in regions associated with action execution, but failed to identify a strong modulation of this activation based on perceived fairness. Multivariate pattern analysis, however, identified clusters potentially carrying information about the perceived fairness of the actor in the middle temporal gyrus, left postcentral gyrus, right inferior parietal lobule, right middle cingulate cortex, right angular gyrus, and right superioroccipital gyrus. Despite being identified by a whole-brain searchlight analysis (and so without anatomical restriction), these clusters fall into areas frequently associated with action observation. We conclude that brain activity during action observation may be modulated by perceived fairness, but such modulation is subtle; robust activity is associated with observing the actions of both fair and unfair individuals.

  5. Is Brain Activity during Action Observation Modulated by the Perceived Fairness of the Actor?

    PubMed

    Etzel, Joset A; Valchev, Nikola; Gazzola, Valeria; Keysers, Christian

    2016-01-01

    Perceiving other people's actions triggers activity in premotor and parietal areas, brain areas also involved in executing and sensing our own actions. Paralleling this phenomenon, observing emotional states (including pain) in others is associated with activity in the same brain areas as activated when experiencing similar emotions directly. This emotion perception associated activity has been shown to be affected by the perceived fairness of the actor, and in-group membership more generally. Here, we examine whether action observation associated brain activity is also affected by the perceived social fairness of the actors. Perceived fairness was manipulated using an alternating iterated Prisoner's Dilemma game between the participant and two confederates, one of whom played fairly and the other unfairly. During fMRI scanning the participants watched movies of the confederates performing object-directed hand actions, and then performed hand actions themselves. Mass-univariate analysis showed that observing the actions triggered robust activation in regions associated with action execution, but failed to identify a strong modulation of this activation based on perceived fairness. Multivariate pattern analysis, however, identified clusters potentially carrying information about the perceived fairness of the actor in the middle temporal gyrus, left postcentral gyrus, right inferior parietal lobule, right middle cingulate cortex, right angular gyrus, and right superioroccipital gyrus. Despite being identified by a whole-brain searchlight analysis (and so without anatomical restriction), these clusters fall into areas frequently associated with action observation. We conclude that brain activity during action observation may be modulated by perceived fairness, but such modulation is subtle; robust activity is associated with observing the actions of both fair and unfair individuals. PMID:26820995

  6. Is Brain Activity during Action Observation Modulated by the Perceived Fairness of the Actor?

    PubMed Central

    Gazzola, Valeria; Keysers, Christian

    2016-01-01

    Perceiving other people’s actions triggers activity in premotor and parietal areas, brain areas also involved in executing and sensing our own actions. Paralleling this phenomenon, observing emotional states (including pain) in others is associated with activity in the same brain areas as activated when experiencing similar emotions directly. This emotion perception associated activity has been shown to be affected by the perceived fairness of the actor, and in-group membership more generally. Here, we examine whether action observation associated brain activity is also affected by the perceived social fairness of the actors. Perceived fairness was manipulated using an alternating iterated Prisoner’s Dilemma game between the participant and two confederates, one of whom played fairly and the other unfairly. During fMRI scanning the participants watched movies of the confederates performing object-directed hand actions, and then performed hand actions themselves. Mass-univariate analysis showed that observing the actions triggered robust activation in regions associated with action execution, but failed to identify a strong modulation of this activation based on perceived fairness. Multivariate pattern analysis, however, identified clusters potentially carrying information about the perceived fairness of the actor in the middle temporal gyrus, left postcentral gyrus, right inferior parietal lobule, right middle cingulate cortex, right angular gyrus, and right superioroccipital gyrus. Despite being identified by a whole-brain searchlight analysis (and so without anatomical restriction), these clusters fall into areas frequently associated with action observation. We conclude that brain activity during action observation may be modulated by perceived fairness, but such modulation is subtle; robust activity is associated with observing the actions of both fair and unfair individuals. PMID:26820995

  7. Corrective Action Investigation Plan for Corrective Action Unit 406: Area 3 Building 03-74 and Building 03-58 Under ground Discharge Points and Corrective Action Unit 429: Area 3 Building 03-55 and Area 9 Building 09-52 Underground Discharge Points, Tonopah Test Range, Nevada

    SciTech Connect

    DOE /NV

    1999-05-20

    This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO (1996), CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites. Corrective Action Units consist of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at the Underground Discharge Points (UDPs) included in both CAU 406 and CAU 429. The CAUs are located in Area 3 and Area 9 of the Tonopah Test Range (TTR). The TTR, included in the Nellis Air Force Range, is approximately 255 kilometers (km) (140 miles [mi]) northwest of Las Vegas, Nevada.

  8. Investigating Nitrogen Pollution: Activities and Models.

    ERIC Educational Resources Information Center

    Green Teacher, 2000

    2000-01-01

    Introduces activities on nitrogen, nitrogen pollution from school commuters, nitrogen response in native and introduced species, and nutrient loading models. These activities help students determine the nitrogen contribution from their parents' cars, test native plant responses to nitrogen, and experiment with the results of removing water from…

  9. Investigation of the mechanisms of action behind Electromotive Drug Administration (EMDA)

    PubMed Central

    Vásquez, Juan Luis; Miklavčič, Damijan; Hermann, Gregers G.G.; Gehl, Julie

    2016-01-01

    Objective Bladder cancer is a cause of considerable morbidity worldwide. Electromotive Drug Administration is a method that combines intravesical chemotherapy with local electric field application. Electroporation has been suggested among other mechanisms as having a possible role in the therapy, so the goal of the present study was to investigate the electric fields present in the bladder wall during the treatment to determine which mechanisms might be involved. Material and Methods Electromotive Drug Administration involves applying intravesical mitomycin C with direct current of 20 mA delivered through a catheter electrode for 30 min. For numerical electric field computation we built a 3-D nonhomogeneous patient specific model based on CT images and used finite element method simulations to determine the electric fields in the whole body. Results Results indicate that highest electric field in the bladder wall was 37.7 V/m. The mean electric field magnitude in the bladder wall was 3.03 V/m. The mean magnitude of the current density in the bladder wall was 0.61 A/m2. Conclusions The present study shows that electroporation is not the mechanism of action in EMDA. A more likely explanation of the mechanism of action is iontophoretic forces increasing the mitomycin C concentration in the bladder wall. PMID:27635313

  10. Investigation of the mechanisms of action behind Electromotive Drug Administration (EMDA)

    PubMed Central

    Vásquez, Juan Luis; Miklavčič, Damijan; Hermann, Gregers G.G.; Gehl, Julie

    2016-01-01

    Objective Bladder cancer is a cause of considerable morbidity worldwide. Electromotive Drug Administration is a method that combines intravesical chemotherapy with local electric field application. Electroporation has been suggested among other mechanisms as having a possible role in the therapy, so the goal of the present study was to investigate the electric fields present in the bladder wall during the treatment to determine which mechanisms might be involved. Material and Methods Electromotive Drug Administration involves applying intravesical mitomycin C with direct current of 20 mA delivered through a catheter electrode for 30 min. For numerical electric field computation we built a 3-D nonhomogeneous patient specific model based on CT images and used finite element method simulations to determine the electric fields in the whole body. Results Results indicate that highest electric field in the bladder wall was 37.7 V/m. The mean electric field magnitude in the bladder wall was 3.03 V/m. The mean magnitude of the current density in the bladder wall was 0.61 A/m2. Conclusions The present study shows that electroporation is not the mechanism of action in EMDA. A more likely explanation of the mechanism of action is iontophoretic forces increasing the mitomycin C concentration in the bladder wall.

  11. Mechanistic investigations on the antioxidant action of a neuroprotective estrogen derivative

    PubMed Central

    Prokai-Tatrai, Katalin; Perjesi, Pal; Rivera-Portalatin, Nilka M.; Simpkins, James W.; Prokai, Laszlo

    2008-01-01

    Antioxidant action is an important component of the complex neuroprotective action of estrogens. Combining theoretical prediction and subsequent experimental confirmation by chemical and in vitro paradigms, this study focused on the mechanistic aspects of hydroxyl-radical scavenging by 17β-butoxy-1,3,5(10)-estratrien-3-ol, a synthetic derivative of 17β-estradiol with increased potency to inhibit lipid peroxidation and reduced affinity to estrogen-receptors compared to the endogenous hormone. In the process that acts as a “chemical shield,” the phenolic A-ring turns into 10β-hydroxy-17β-butoxy-1,3,5(10)-estratrien-3-one, a non-aromatic para-quinol, upon capturing hydroxyl-radicals, which results in the complete loss of estrogen-receptor affinity and antioxidant activity. However, the parent compound is apparently recovered in brain tissue from this para-quinol via enzyme-catalyzed NAD(P)H-dependent reductive aromatization without causing oxidative stress. Taken together, our report argues for a previously unrecognized antioxidant cycle for synthetic estrogen-derived compounds. PMID:18068745

  12. Corrective Action Investigation Plan for Corrective Action Unit 214: Bunkers and Storage Areas Nevada Test Site, Nevada: Revision 0, Including Record of Technical Change No. 1 and No. 2

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2003-05-16

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 214 under the Federal Facility Agreement and Consent Order. Located in Areas 5, 11, and 25 of the Nevada Test Site, CAU 214 consists of nine Corrective Action Sites (CASs): 05-99-01, Fallout Shelters; 11-22-03, Drum; 25-99-12, Fly Ash Storage; 25-23-01, Contaminated Materials; 25-23-19, Radioactive Material Storage; 25-99-18, Storage Area; 25-34-03, Motor Dr/Gr Assembly (Bunker); 25-34-04, Motor Dr/Gr Assembly (Bunker); and 25-34-05, Motor Dr/Gr Assembly (Bunker). These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). The suspected contaminants and critical analyte s for CAU 214 include oil (total petroleum hydrocarbons-diesel-range organics [TPH-DRO], polychlorinated biphenyls [PCBs]), pesticides (chlordane, heptachlor, 4,4-DDT), barium, cadmium, chronium, lubricants (TPH-DRO, TPH-gasoline-range organics [GRO]), and fly ash (arsenic). The land-use zones where CAU 214 CASs are located dictate that future land uses will be limited to nonresidential (i.e., industrial) activities. The results of this field investigation will support a defensible evaluation of viable corrective action alternatives that will be presented in the corrective action decision document.

  13. 76 FR 20707 - Notice of Possible Shutdown of Investigative Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... determined to shut down its investigative activities in the event of the absence of an appropriation. FOR... shut down its investigative activities for the duration of the absence of appropriation....

  14. Corrective Action Investigation Plan for Corrective Action Unit 321: Area 22 Weather Station Fuel Storage, Nevada Test Site, Nevada, Revision 0. UPDATED WITH RECORD OF TECHNICAL CHANGE No.1

    SciTech Connect

    U.S. DOE /NV

    1999-02-08

    This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO (1996), CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites. A CAU consists of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at the CAU 321 Area 22 Weather Station Fuel Storage, CAS 22-99-05 Fuel Storage Area. For purposes of this discussion, this site will be referred to as either CAU 321 or the Fuel Storage Area. The Fuel Storage Area is located in Area 22 of the Nevada Test Site (NTS). The NTS is approximately 105 kilometers (km) (65 miles [mi]) northwest of Las Vegas, Nevada (DOE/NV, 1996a). The Fuel Storage Area was used to store fuel and other petroleum products necessary for motorized operations at the historic Camp Desert Rock facility which was operational from 1951 to 1958 at the Nevada Test Site, Nevada. The site was dismantled after 1958 (DOE/NV, 1996a).

  15. Health activism and the logic of connective action. A case study of rare disease patient organisations

    PubMed Central

    Vicari, Stefania; Cappai, Franco

    2016-01-01

    ABSTRACT This exploratory work investigates the role of digital media in expanding health discourse practices in a way to transform traditional structures of agency in public health. By focusing on a sample of rare disease patient organisations as representative of contemporary health activism, this study investigates the role of digital communication in the development of (1) bottom-up sharing and co-production of health knowledge, (2) health public engagement dynamics and (3) health information pathways. Findings show that digital media affordances for patient organisations go beyond the provision of social support for patient communities; they ease one-way, two-way and crowdsourced processes of health knowledge sharing, exchange and co-production, provide personalised routes to health public engagement and bolster the emergence of varied pathways to health information where experiential knowledge and medical authority are equally valued. These forms of organisationally enabled connective action can help the surfacing of personal narratives that strengthen patient communities, the bottom-up production of health knowledge relevant to a wider public and the development of an informational and eventually cultural context that eases patients’ political action. PMID:27499676

  16. Antifungal activity of salicylic acid against Penicillium expansum and its possible mechanisms of action.

    PubMed

    da Rocha Neto, Argus Cezar; Maraschin, Marcelo; Di Piero, Robson Marcelo

    2015-12-23

    Apple is a fruit widely produced and consumed around the world. Blue mold (Penicillium expansum) is one of the main postharvest diseases in apples, leading to a wide use of fungicides and the search for alternative products. The aim of this study was to assess the effect of salicylic acid (SA) against P. expansum, elucidating its mechanisms of action. The antimicrobial effect was determined by exposing conidia to a 2.5 mM SA solution for 0 to 120 min, followed by incubation. The effect of pH on the efficacy of SA against P. expansum was assessed both in vitro and in situ. The action mechanisms were investigated through fluorescence assays, measurement of protein leakage, lipid damage, and transmission electronic microscopy. SA was capable of inhibiting 90% of the fungal germination after 30 min, causing damage to the conidial plasma membrane and leading to protein leakage up to 3.2 μg of soluble protein per g of mycelium. The pH of the SA solution affected the antimicrobial activity of this secondary metabolite, which inhibited the germination of P. expansum and the blue mold incidence in apples in solutions with pH≤3 by 100%, gradually losing its activity at higher pH.

  17. Anti-diabetic action of Punica granatum flower extract: Activation of PPAR-{gamma} and identification of an active component

    SciTech Connect

    Huang, Tom H.W.; Peng Gang; Kota, Bhavani P.; Li, George Q.; Yamahara, Johji; Roufogalis, Basil D.; Li Yuhao . E-mail: yuhao@pharm.usyd.edu.au

    2005-09-01

    Peroxisome proliferator-activated receptor (PPAR)-{gamma} activators are widely used in the treatment of type 2 diabetes because they improve the sensitivity of insulin receptors. Punica granatum flower (PGF) has been used as an anti-diabetic medicine in Unani medicinal literature. The mechanism of actions is, however, unknown. In the current study, we demonstrated that 6-week oral administration of methanol extract from PGF (500 mg/kg, daily) inhibited glucose loading-induced increase of plasma glucose levels in Zucker diabetic fatty rats (ZDF), a genetic animal model for type 2 diabetes, whereas it did not inhibit the increase in Zucker lean rats (ZL). The treatment did not lower the plasma glucose levels in fasted ZDF and ZL rats. Furthermore, RT-PCR results demonstrated that the PGF extract treatment in ZDF rats enhanced cardiac PPAR-{gamma} mRNA expression and restored the down-regulated cardiac glucose transporter (GLUT)-4 (the insulin-dependent isoform of GLUTs) mRNA. These results suggest that the anti-diabetic activity of PGF extract may result from improved sensitivity of the insulin receptor. From the in vitro studies, we demonstrated that the PGF extract enhanced PPAR-{gamma} mRNA and protein expression and increased PPAR-{gamma}-dependent mRNA expression and activity of lipoprotein lipase in human THP-1-differentiated macrophage cells. Phytochemical investigation demonstrated that gallic acid in PGF extract is mostly responsible for this activity. Thus, our findings indicate that PPAR-{gamma} is a molecular target for PGF extract and its prominent component gallic acid, and provide a better understanding of the potential mechanism of the anti-diabetic action of PGF.

  18. Investigation into stereoselective pharmacological activity of phenotropil.

    PubMed

    Zvejniece, Liga; Svalbe, Baiba; Veinberg, Grigory; Grinberga, Solveiga; Vorona, Maksims; Kalvinsh, Ivars; Dambrova, Maija

    2011-11-01

    Phenotropil [N-carbamoylmethyl-4-aryl-2-pyrrolidone (2-(2-oxo-4-phenyl-pyrrolidin-1-yl) acetamide; carphedon)] is clinically used in its racemic form as a nootropic drug that improves physical condition and cognition. The aim of this study was to compare the stereoselective pharmacological activity of R- and S-enantiomers of phenotropil in different behavioural tests. Racemic phenotropil and its enantiomers were tested for locomotor, antidepressant and memory-improving activity and influence on the central nervous system (CNS) using general pharmacological tests in mice. After a single administration, the amount of compound in brain tissue extracts was determined using an ultra performance liquid chromatography-tandem mass spectrometry (UPLC/MS/MS) method in a positive ion electrospray mode. In the open-field test, a significant increase in locomotor activity was observed after a single administration of R-phenotropil at doses of 10 and 50 mg/kg and S-phenotropil at a dose of 50 mg/kg. In the forced swim test, R-phenotropil induced an antidepressant effect at doses of 100 and 50 mg/kg, and S-phenotropil was active at a dose of 100 mg/kg. R-phenotropil significantly enhanced memory function in a passive avoidance response test at a dose of 1 mg/kg; the S-enantiomer did not show any activity in this test. However, the concentrations of R- and S-phenotropils in brain tissue were similar. In conclusion, the antidepressant and increased locomotor activity relies on both R- and S-phenotropils, but the memory-improving activity is only characteristic of R-phenotropil. These results may be important for the clinical use of optically pure isomers of phenotropil.

  19. [Investigation of physical activity among adults].

    PubMed

    Meboniia, N M; Kalandadze, I L; Chachava, T D; Sadzhaia, M V

    2006-06-01

    Lack of physical activity as well as smoking, overweight, high blood cholesterol level and hypertension are independent risk factors for development of various chronic diseases. Lack of physical activity is a main cause of non-communicable diseases morbidity and mortality in about 23% of cases (WHO, 2002). Goal of the survey was the identification and characteristics of physical activity among adults. We conducted epidemiologic survey among the school-children and students in Tbilisi. Survey was carried out in 40 schools from all regions of Tbilisi and 9 state and private institutes. Schools were selected by randomized trail in each region of Tbilisi and in each school were questioned all teen-agers from 14-17 year old. For selecting group of survey was used method of "proportional probability of value". There were questioned 2 500 school children and 1000 students, 38% boys and 62% girls. The results of survey ascertained that 23,5%-39,5% of respondents are training or engaged in sport everyday, or several times per week; 17,2% - once in a month; 9,3% once in a year; or 10,5% - never. Adults are less informed about useful effect of physical activity. Respondents had different point of on the role of physical activity: some of them thought it controls weight; others - prevents development of different diseases; the rest thinks that it copes with psycho-emotional stress, and none of them precise about effectiveness of physical activity in all cases mentioned above. Propaganda of healthy life stile must be associated with the effectiveness of physical activity on health.

  20. State-Level Activities: A Plan for Action.

    ERIC Educational Resources Information Center

    Lamborn, Robert L.

    A variety of specific activities that have been successfully undertaken by the Council for American Private Education (CAPE) are suggested for state members. The activities are related to developing private school communications within states and developing working relationships with local, metropolitan, and state governments and their agencies.…

  1. Narratives and Activity Theory as Reflective Tools in Action Research

    ERIC Educational Resources Information Center

    Stuart, Kaz

    2012-01-01

    Narratives and activity theory are useful as socially constructed data collection tools that allow a researcher access to the social, cultural and historical meanings that research participants place on events in their lives. This case study shows how these tools were used to promote reflection within a cultural-historical activity theoretically…

  2. Immune activation by a sterile aqueous extract of Cordyceps sinensis: mechanism of action.

    PubMed

    Jordan, J L; Sullivan, A M; Lee, T D G

    2008-01-01

    Cordyceps sinensis is a fungus that has been used for over 2,000 years in China as a treatment for a variety of conditions including infectious diseases. The available evidence suggests a hypothesis that any efficacy of C. sinensis as an anti-infective therapeutic would be related to a role as an activator of innate immune responses. The objectives of this study were first to investigate the ability of C. sinensis to activate pro-inflammatory responses in macrophages in vitro and induce protective responses against intracellular pathogens in vivo, and second to characterize a method of action. We found that C. sinensis activates murine macrophages to produce a variety of pro-inflammatory cytokines. IFN-gamma synergizes with C. sinensis to amplify this response. Bacterial endotoxin contamination was ruled out as a potential artefact. The evidence presented in this study supports a hypothesis that C. sinensis activates macrophages by engaging Toll-like receptors and inducing mitogen-activated protein kinase (MAPK) pathways characteristic of inflammatory stimuli.

  3. Antibacterial action of silver-doped activated carbon prepared by vacuum impregnation

    NASA Astrophysics Data System (ADS)

    Zhao, Ying; Wang, Zi-qiang; Zhao, Xin; Li, Wei; Liu, Shou-xin

    2013-02-01

    Silver-containing activated carbon (Ag/AC) exhibiting controlled release of silver and antibacterial action was prepared by vacuum impregnation using acetate silver as a precursor. The antibacterial activity toward E. coli and resistance to water erosion were investigated with the view of water purification. N2 adsorption at 77 K, scanning electron microscopy and X-ray diffraction were used to characterize the surface morphology and crystalline properties of the Ag/AC samples. As the concentration of CH3COOAg increased, the samples change from exhibiting no antibacterial activity to inhibition of bacteria growth and then to antibacterial activity because of the higher silver content and smaller size of the silver particles. The Ag/AC composites showed a lower release rate of silver than that of a composite prepared by a traditional AgNO3 impregnation method, which suggests a strong interaction between the silver particles and carbon. Because the Ag particles block the pores of AC, the BET surface area, total pore volume and average pore diameter of the Ag/AC samples decreased as the concentration of the CH3COOAg solution increased. The higher antibacterial activity and controlled release of silver by Ag/AC containing 1.65 wt % silver means that it shows promise for purification of drinking water.

  4. Modulation of neural activity during observational learning of actions and their sequential orders.

    PubMed

    Frey, Scott H; Gerry, Valerie E

    2006-12-20

    How does the brain transform perceptual representations of others' actions into motor representations that can be used to guide behavior? Here we used functional magnetic resonance imaging to record human brain activity while subjects watched others construct multipart objects under varied task demands. We find that relative to resting baseline, passive action observation increases activity within inferior frontal and parietal cortices implicated in action encoding (mirror system) and throughout a distributed network of areas involved in motor representation, including dorsal premotor cortex, pre-supplementary motor area, cerebellum, and basal ganglia (experiments 1 and 2). Relative to passive observation, these same areas show increased activity when subjects observe with the intention to subsequently reproduce component actions using the demonstrated sequential procedures (experiment 1). Observing the same actions with the intention of reproducing component actions, but without the requirement to use the demonstrated sequential procedure, increases activity in the same regions, although to a lesser degree (experiment 2). These findings demonstrate that when attempting to learn behaviors through observation, the observers' intentions modulate responses in a widely distributed network of cortical and subcortical regions implicated previously in action encoding and/or motor representation. Among these regions, only activity within the right intraparietal sulcus predicts the accuracy with which observed procedures are subsequently performed. Successful formation of motor representations of sequential procedures through observational learning is dependent on computations implemented within this parietal region. PMID:17182769

  5. Physical activity counselling in sports medicine: a call to action.

    PubMed

    Joy, Elizabeth Liz; Blair, Steven N; McBride, Patrick; Sallis, Robert

    2013-01-01

    Physical activity (PA) is a key component of healthy lifestyle and disease prevention. In contrast, physical inactivity accounts for a significant proportion of premature deaths worldwide. Physicians are in a critical position to help patients develop healthy lifestyles by actively counseling on PA. Sports medicine physicians, with their focus on sports and exercise medicine are uniquely trained to provide such expertise to patients, learners and colleagues. To succeed, physicians need clinical tools and processes that support PA assessment and counseling. Linking patients to community resources, and specifically to health and fitness professionals is a key strategy. Efforts should be made to expand provider education during medical school, residency and fellowship training, and continuing medical education. Lastly, physically active physicians are more likely to counsel patients to be active. A key message for the sports medicine community is the importance of serving as a positive PA role model.

  6. Corrective Action Investigation Plan for Corrective Action Unit 234: Mud Pits, Cellars, and Mud Spills, Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Grant Evenson

    2007-08-01

    Corrective Action Unit 234, Mud Pits, Cellars, and Mud Spills, consists of 12 inactive sites located in the north and northeast section of the NTS. The 12 CAU 234 sites consist of mud pits, mud spills, mud sumps, and an open post-test cellar. The CAU 234 sites were all used to support nuclear testing conducted in the Yucca Flat and Rainier Mesa areas during the 1950s through the 1970s. The CASs in CAU 234 are being investigated because hazardous and/or radioactive constituents may be present in concentrations that could potentially pose a threat to human health and the environment. Existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives for the CASs. Additional information will be generated by conducting a CAI before evaluating and selecting appropriate corrective action alternatives.

  7. 76 FR 44339 - Office of Biotechnology Activities; Recombinant DNA Research: Action Under the NIH Guidelines for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health Office of Biotechnology Activities; Recombinant DNA...) AGENCY: National Institutes of Health, PHS, Department of Health and Human Services. ACTION:...

  8. Investigations of the Mode of Action and Resistance Development of Cadazolid, a New Antibiotic for Treatment of Clostridium difficile Infections

    PubMed Central

    Caspers, Patrick; Bruyère, Thierry; Schroeder, Susanne; Pfaff, Philippe; Knezevic, Andreja; Keck, Wolfgang; Ritz, Daniel

    2014-01-01

    Cadazolid is a new oxazolidinone-type antibiotic currently in clinical development for the treatment of Clostridium difficile-associated diarrhea. Here, we report investigations on the mode of action and the propensity for spontaneous resistance development in C. difficile strains. Macromolecular labeling experiments indicated that cadazolid acts as a potent inhibitor of protein synthesis, while inhibition of DNA synthesis was also observed, albeit only at substantially higher concentrations of the drug. Strong inhibition of protein synthesis was also obtained in strains resistant to linezolid, in agreement with low MICs against such strains. Inhibition of protein synthesis was confirmed in coupled transcription/translation assays using extracts from different C. difficile strains, including strains resistant to linezolid, while inhibitory effects in DNA topoisomerase assays were weak or not detectable under the assay conditions. Spontaneous resistance frequencies of cadazolid were low in all strains tested (generally <10−10 at 2× to 4× the MIC), and in multiple-passage experiments (up to 13 passages) MICs did not significantly increase. Furthermore, no cross-resistance was observed, as cadazolid retained potent activity against strains resistant or nonsusceptible to linezolid, fluoroquinolones, and the new antibiotic fidaxomicin. In conclusion, the data presented here indicate that cadazolid acts primarily by inhibition of protein synthesis, with weak inhibition of DNA synthesis as a potential second mode of action, and suggest a low potential for spontaneous resistance development. PMID:24277035

  9. Investigations of the mode of action and resistance development of cadazolid, a new antibiotic for treatment of Clostridium difficile infections.

    PubMed

    Locher, Hans H; Caspers, Patrick; Bruyère, Thierry; Schroeder, Susanne; Pfaff, Philippe; Knezevic, Andreja; Keck, Wolfgang; Ritz, Daniel

    2014-01-01

    Cadazolid is a new oxazolidinone-type antibiotic currently in clinical development for the treatment of Clostridium difficile-associated diarrhea. Here, we report investigations on the mode of action and the propensity for spontaneous resistance development in C. difficile strains. Macromolecular labeling experiments indicated that cadazolid acts as a potent inhibitor of protein synthesis, while inhibition of DNA synthesis was also observed, albeit only at substantially higher concentrations of the drug. Strong inhibition of protein synthesis was also obtained in strains resistant to linezolid, in agreement with low MICs against such strains. Inhibition of protein synthesis was confirmed in coupled transcription/translation assays using extracts from different C. difficile strains, including strains resistant to linezolid, while inhibitory effects in DNA topoisomerase assays were weak or not detectable under the assay conditions. Spontaneous resistance frequencies of cadazolid were low in all strains tested (generally <10(-10) at 2× to 4× the MIC), and in multiple-passage experiments (up to 13 passages) MICs did not significantly increase. Furthermore, no cross-resistance was observed, as cadazolid retained potent activity against strains resistant or nonsusceptible to linezolid, fluoroquinolones, and the new antibiotic fidaxomicin. In conclusion, the data presented here indicate that cadazolid acts primarily by inhibition of protein synthesis, with weak inhibition of DNA synthesis as a potential second mode of action, and suggest a low potential for spontaneous resistance development.

  10. Disruption of Saccharomyces cerevisiae by Plantaricin 149 and investigation of its mechanism of action with biomembrane model systems.

    PubMed

    Lopes, José Luiz S; Nobre, Thatyane M; Siano, Alvaro; Humpola, Verónica; Bossolan, Nelma R S; Zaniquelli, Maria E D; Tonarelli, Georgina; Beltramini, Leila M

    2009-10-01

    The action of a synthetic antimicrobial peptide analog of Plantaricin 149 (Pln149a) against Saccharomyces cerevisiae and its interaction with biomembrane model systems were investigated. Pln149a was shown to inhibit S. cerevisiae growth by more than 80% in YPD medium, causing morphological changes in the yeast wall and remaining active and resistant to the yeast proteases even after 24 h of incubation. Different membrane model systems and carbohydrates were employed to better describe the Pln149a interaction with cellular components using circular dichroism and fluorescence spectroscopies, adsorption kinetics and surface elasticity in Langmuir monolayers. These assays showed that Pln149a does not interact with either mono/polysaccharides or zwitterionic LUVs, but is strongly adsorbed to and incorporated into negatively charged surfaces, causing a conformational change in its secondary structure from random-coil to helix upon adsorption. From the concurrent analysis of Pln149a adsorption kinetics and dilatational surface elasticity data, we determined that 2.5 muM is the critical concentration at which Pln149a will disrupt a negative DPPG monolayer. Furthermore, Pln149a exhibited a carpet-like mechanism of action, in which the peptide initially binds to the membrane, covering its surface and acquiring a helical structure that remains associated to the negatively charged phospholipids. After this electrostatic interaction, another peptide region causes a strain in the membrane, promoting its disruption.

  11. Climate Action and Activism: Scientists as Citizens and Communicators

    NASA Astrophysics Data System (ADS)

    Brown, M. B.; Peacock, K.

    2015-12-01

    Humans are not particularly good at being rational, either individually or socially; in the case of climate change, our concerns are chiefly social. The denial of climate change and its costs (ranging from denial of basic principles to using high discount rates to reduce the current value of future losses, and supported by fossil fuel companies and their many political allies) has delayed an effective response to a problem that gets worse and more costly the longer action is delayed. The central role of fossil fuels in our economies and of fossil fuel interests in our politics leads many to worry about the costs of change while denying or ignoring the costs of business as usual. Rational decision makers would not be so selective, either about the evidence or about the costs and benefits that hang in the balance. Effective communication can help call attention to the evidence and to the costs and benefits that have been neglected. Our society has no formal rules requiring scientists to become activists, even when the results of their work provide sound reasons for taking action. But ideals of citizenship and humanitarianism provide strong justification for those who choose to engage with the issues. A reticent scientist might feel that her job is done once the results of her research are published. The rest is arguably the responsibility of others—of politicians, journalists and citizens in general, to learn the relevant facts (now available as part of the published literature) and to bring those facts to bear in decisions ranging from the personal to the political and economic. But I urge scientists who feel this way to reconsider—not because their view of where the real responsibility lies is wrong, but because they are in a position to make a difference. In situations like these, where powerful interests are threatened by inconvenient facts, scientists can be very effective communicators: they have high credibility with the public (as deniers' repeated claims

  12. Corrective Action Investigation Plan for Corrective Action Unit 140: Waste Dumps, Burn Pits, and Storage Area, Nevada Test Site, Nevada, July 2002, Rev. No. 0

    SciTech Connect

    NNSA /NV

    2002-07-18

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 140 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 140 consists of nine Corrective Action Sites (CASs): 05-08-01, Detonation Pits; 05-08-02, Debris Pits; 05-17-01, Hazardous Waste Accumulation Site (Buried); 05-19-01, Waste Disposal Site; 05-23-01, Gravel Gertie; 05-35-01, Burn Pit; 05-99-04, Burn Pit; 22-99-04, Radioactive Waste Dump; 23-17-01, Hazardous Waste Storage Area. All nine of these CASs are located within Areas 5, 22, and 23 of the Nevada Test Site (NTS) in Nevada, approximately 65 miles northwest of Las Vegas. This CAU is being investigated because disposed waste may be present without appropriate controls (i.e., use restrictions, adequate cover) and hazardous and/or radioactive constituents may be present or migrating at concentrations and locations that could potentially pose a threat to human health and the environment. The NTS has been used for various research and development projects including nuclear weapons testing. The CASs in CAU 140 were used for testing, material storage, waste storage, and waste disposal. A two-phase approach has been selected to collect information and generate data to satisfy needed resolution criteria and resolve the decision statements. Phase I will determine if contaminants of potential concern (COPCs) are present in concentrations exceeding preliminary action levels. This data will be evaluated at all CASs. Phase II will determine the extent of the contaminant(s) of concern (COCs). This data will only be evaluated for CASs with a COC identified during Phase I. Based on process knowledge, the COPCs for CAU 140 include volatile organics, semivolatile organics, petroleum hydrocarbons, explosive residues

  13. Radioprotective cerium oxide nanoparticles: Molecular imaging investigations of conps' pharmacokinetics, efficacy, and mechanisms of action

    NASA Astrophysics Data System (ADS)

    McDonagh, Philip Reed Wills, III

    Cerium oxide nanoparticles (CONPs) are being investigated for several anti-oxidant applications in medicine. One of their most promising applications is as a radioprotective drug, an area of research in need due to the severe side effects from radiation therapy. In this work, the potential of CONPs as a radioprotective drug is examined using four criteria: favorable biodistribution/pharmacokinetics, low toxicity, ability to protect normal tissue from radiation damage, and lack of protection of tumor. The mechanisms of action of CONPs are also studied. Biodistribution was determined in radiolabeled CONPs with surface coatings including citrate, dextran T10-amine (DT10-NH2), dextran T10-polyethylene glycol (DT10-PEG), dextran T10-sulfobetaine (DT10-SB) and poly(acrylic acid) (PAA), and compared to uncoated. 89Zr was incorporated into CONPs for positron emission tomography (PET) imaging and ex vivo tissue analysis in tumor bearing mice. Compared to uncoated [ 89Zr]CONPs, coated [89Zr]CONPs showed improved biodistribution, including significantly enhanced renal clearance of PAA- [89Zr]CONPs. The toxicity of CONPs was evaluated in vitro and in vivo, with low toxicity at therapeutic doses. After clinically mimetic radiation therapy, pre-treatment of mice with coated and uncoated CONPs showed greater than 50% reduction of cell death in normal colon tissue, comparable to the clinically available radioprotective drug amifostine. Tumor control after irradiation of spontaneous colon tumors was unchanged with PAA-CONP pre-treatment, while citrate, DT10-PEG, and uncoated CONP pre-treatment had slightly less tumor control. Xenograft tumors were irradiated after pH normalizing treatment with sodium bicarbonate and PAA-CONP pre-treatment. Treatment of these tumors showed slightly less tumor control than irradiation alone or PAA-CONP plus irradiation, demonstrating that the acidic pH of the tumor microenvironment may be the basis of preventing CONPs' radioprotective properties in

  14. Practicum in adapted physical activity: a Dewey-inspired action research project.

    PubMed

    Standal, Øyvind; Rugseth, Gro

    2014-07-01

    The purpose of this study was to investigate what adapted physical activity (APA) students learn from their practicum experiences. One cohort of APA students participated, and data were generated from an action research project that included observations, reflective journals, and a focus group interview. The theoretical framework for the study was Dewey's and Wackerhausen's theories of reflections. The findings show the objects of students' reflections, the kind of conceptual resources they draw on while reflecting, and their knowledge interests. In addition, two paradoxes are identified: the tension between reflecting from and on own values, and how practicum as a valued experience of reality can become too difficult to handle. In conclusion, we reflect on how practicum learning can be facilitated.

  15. Time for Action: Advocacy for Physical Activity in Later Life

    ERIC Educational Resources Information Center

    Grant, Bevan

    2010-01-01

    By 2050, the over 65 year's age group will account for approximately one quarter of the population. This will have many unprecedented social and economic consequences of which one is the cost associated with health. A preventive health related behaviour attracting considerable attention is physical activity, something that becomes less popular…

  16. Educational Leadership and Social Activism: A Call for Action

    ERIC Educational Resources Information Center

    Hoffman, Lauren P.

    2009-01-01

    The purpose of this article is to argue for a social activist stance in educational leadership that fundamentally addresses social change and human emancipation. This call for social activism is framed within neoliberal, neoconservative, and authoritarian populist discourses in the USA, which to social justice educators and leaders had devastating…

  17. Corrective Action Investigation Plan for Corrective Action Unit 569: Area 3 Yucca Flat Atmospheric Test Sites Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews; Christy Sloop

    2012-02-01

    Corrective Action Unit (CAU) 569 is located in Area 3 of the Nevada National Security Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 569 comprises the nine numbered corrective action sites (CASs) and one newly identified site listed below: (1) 03-23-09, T-3 Contamination Area (hereafter referred to as Annie, Franklin, George, and Moth); (2) 03-23-10, T-3A Contamination Area (hereafter referred to as Harry and Hornet); (3) 03-23-11, T-3B Contamination Area (hereafter referred to as Fizeau); (4) 03-23-12, T-3S Contamination Area (hereafter referred to as Rio Arriba); (5) 03-23-13, T-3T Contamination Area (hereafter referred to as Catron); (6) 03-23-14, T-3V Contamination Area (hereafter referred to as Humboldt); (7) 03-23-15, S-3G Contamination Area (hereafter referred to as Coulomb-B); (8) 03-23-16, S-3H Contamination Area (hereafter referred to as Coulomb-A); (9) 03-23-21, Pike Contamination Area (hereafter referred to as Pike); and (10) Waste Consolidation Site 3A. Because CAU 569 is a complicated site containing many types of releases, it was agreed during the data quality objectives (DQO) process that these sites will be grouped. These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each study group. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the DQOs developed on September 26, 2011, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO

  18. Orexin A enhances locomotor activity and induces anxiogenic-like action in the goldfish, Carassius auratus.

    PubMed

    Nakamachi, Tomoya; Shibata, Haruki; Sakashita, Atsushi; Iinuma, Naoto; Wada, Kohei; Konno, Norifumi; Matsuda, Kouhei

    2014-07-01

    Orexin acts as an orexigenic factor for the regulation of appetite and rhythmicity in rodents. In goldfish, intracerebroventricular (ICV) administration of orexin A has been shown to affect not only food intake, but also locomotor activity. However, as there is still no information regarding the effect of orexin A on emotional behavior in goldfish, we investigated the effect of orexin A on psychomotor activity in this species. Intracerebroventricular administration of synthetic orexin A at 2 and 4pmol/g body weight (BW) enhanced locomotor activity, and this enhancement by orexin A at 4pmol/g BW was attenuated by treatment with the orexin receptor 1 antagonist, SB334867, at 10pmol/g BW. Since intact goldfish prefer a black to a white background area, or the lower to the upper area of a tank, we used two types of preference tests (black/white and upper/lower tests) for measuring anxiety-like behavior in goldfish. Intracerebroventricular administration of orexin A at 4pmol/g BW shortened the time spent in the white background area, and increased the time taken to move from the lower to the upper area. This action of orexin A mimicked that of the central-type benzodiazepine receptor inverse agonist, FG-7142 (an anxiogenic agent), at 4pmol/g BW. The anxiogenic-like effect of orexin A was abolished by treatment with SB334867 at 10pmol/g BW. These results indicate that orexin A potently affects psychomotor activity in goldfish.

  19. Corrective Action Investigation Plan for Corrective Action Unit 271: Areas 25, 26, and 27 Septic Systems, Nevada Test Site, Nevada (Rev. 0, April 2001)

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office

    2001-04-09

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 271 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 271 consists of 15 Corrective Action Sites (CASs) including: thirteen Septic Systems (25-04-01, 25-04-03, 25-04-04, 25-04-08, 25-04-09, 25-04-10, 25-04-11, 26-04-01, 26-04-02, 26-05-03, 26-05-04, 26-05-05, and 27-05-02), one Contaminated Water Reservoir (26-03-01), and one Radioactive Leachfield (26-05-01). The CASs addressed by CAU 271 are located at Guard Station 500, the Reactor Control Point (RCP), Bare Reactor Experiment - Nevada Tower, and Engine Test State-1 (ETS-1) facilities in Area 25; the Port Gaston and Project Pluto facilities in Area 26; and the Baker Site in Area 27 of the Nevada Test Site. Between 1 958 and 1973, the RCP and ETS-1 facilities supported the development and testing of nuclear reactors for space propulsion as part of the Nuclear Rocket Development Station. The Project Pluto facilities supported nuclear reactor testing for use as a ramjet propulsion system between 1961 and 1964, followed by similar use for other projects through the early 1980s. The Baker Site facilities were constructed in the 1960s to serve as the staging point where the manufactured components of nuclear devices were assembled, disassembled, and modified. The scope of the investigation strategy at these sites will involve biased and random soil sampling in leachfields using excavation (with drilling as a contingency), collection of soil samples underlying the base of proximal and distal ends of septic tanks and distal ends of distribution structures, defining the lateral and vertical extent of contamination through discrete field and possible stepout location sampling, collection system line

  20. Antinociceptive action of isolated mitragynine from Mitragyna Speciosa through activation of opioid receptor system.

    PubMed

    Shamima, Abdul Rahman; Fakurazi, Sharida; Hidayat, Mohamad Taufik; Hairuszah, Ithnin; Moklas, Mohamad Aris Mohd; Arulselvan, Palanisamy

    2012-01-01

    Cannabinoids and opioids systems share numerous pharmacological properties and antinociception is one of them. Previous findings have shown that mitragynine (MG), a major indole alkaloid found in Mitragyna speciosa (MS) can exert its antinociceptive effects through the opioids system. In the present study, the action of MG was investigated as the antinociceptive agent acting on Cannabinoid receptor type 1 (CB1) and effects on the opioids receptor. The latency time was recorded until the mice showed pain responses such as shaking, licking or jumping and the duration of latency was measured for 2 h at every 15 min interval by hot plate analysis. To investigate the beneficial effects of MG as antinociceptive agent, it was administered intraperitoneally 15 min prior to pain induction with a single dosage (3, 10, 15, 30, and 35 mg/kg b.wt). In this investigation, 35 mg/kg of MG showed significant increase in the latency time and this dosage was used in the antagonist receptor study. The treated groups were administered with AM251 (cannabinoid receptor-1 antagonist), naloxone (non-selective opioid antagonist), naltrindole (δ-opioid antagonist) naloxonazine (μ(1)-receptor antagonist) and norbinaltorpimine (κ-opioid antagonist) respectively, prior to administration of MG (35 mg/kg). The results showed that the antinociceptive effect of MG was not antagonized by AM251; naloxone and naltrindole were effectively blocked; and norbinaltorpimine partially blocked the antinociceptive effect of MG. Naloxonazine did inhibit the effect of MG, but it was not statistically significant. These results demonstrate that CB1 does not directly have a role in the antinociceptive action of MG where the effect was observed with the activation of opioid receptor. PMID:23109863

  1. Antinociceptive Action of Isolated Mitragynine from Mitragyna Speciosa through Activation of Opioid Receptor System

    PubMed Central

    Shamima, Abdul Rahman; Fakurazi, Sharida; Hidayat, Mohamad Taufik; Hairuszah, Ithnin; Moklas, Mohamad Aris Mohd; Arulselvan, Palanisamy

    2012-01-01

    Cannabinoids and opioids systems share numerous pharmacological properties and antinociception is one of them. Previous findings have shown that mitragynine (MG), a major indole alkaloid found in Mitragyna speciosa (MS) can exert its antinociceptive effects through the opioids system. In the present study, the action of MG was investigated as the antinociceptive agent acting on Cannabinoid receptor type 1 (CB1) and effects on the opioids receptor. The latency time was recorded until the mice showed pain responses such as shaking, licking or jumping and the duration of latency was measured for 2 h at every 15 min interval by hot plate analysis. To investigate the beneficial effects of MG as antinociceptive agent, it was administered intraperitoneally 15 min prior to pain induction with a single dosage (3, 10, 15, 30, and 35 mg/kg b.wt). In this investigation, 35 mg/kg of MG showed significant increase in the latency time and this dosage was used in the antagonist receptor study. The treated groups were administered with AM251 (cannabinoid receptor-1 antagonist), naloxone (non-selective opioid antagonist), naltrindole (δ-opioid antagonist) naloxonazine (μ1-receptor antagonist) and norbinaltorpimine (κ-opioid antagonist) respectively, prior to administration of MG (35 mg/kg). The results showed that the antinociceptive effect of MG was not antagonized by AM251; naloxone and naltrindole were effectively blocked; and norbinaltorpimine partially blocked the antinociceptive effect of MG. Naloxonazine did inhibit the effect of MG, but it was not statistically significant. These results demonstrate that CB1 does not directly have a role in the antinociceptive action of MG where the effect was observed with the activation of opioid receptor. PMID:23109863

  2. Antinociceptive action of isolated mitragynine from Mitragyna Speciosa through activation of opioid receptor system.

    PubMed

    Shamima, Abdul Rahman; Fakurazi, Sharida; Hidayat, Mohamad Taufik; Hairuszah, Ithnin; Moklas, Mohamad Aris Mohd; Arulselvan, Palanisamy

    2012-01-01

    Cannabinoids and opioids systems share numerous pharmacological properties and antinociception is one of them. Previous findings have shown that mitragynine (MG), a major indole alkaloid found in Mitragyna speciosa (MS) can exert its antinociceptive effects through the opioids system. In the present study, the action of MG was investigated as the antinociceptive agent acting on Cannabinoid receptor type 1 (CB1) and effects on the opioids receptor. The latency time was recorded until the mice showed pain responses such as shaking, licking or jumping and the duration of latency was measured for 2 h at every 15 min interval by hot plate analysis. To investigate the beneficial effects of MG as antinociceptive agent, it was administered intraperitoneally 15 min prior to pain induction with a single dosage (3, 10, 15, 30, and 35 mg/kg b.wt). In this investigation, 35 mg/kg of MG showed significant increase in the latency time and this dosage was used in the antagonist receptor study. The treated groups were administered with AM251 (cannabinoid receptor-1 antagonist), naloxone (non-selective opioid antagonist), naltrindole (δ-opioid antagonist) naloxonazine (μ(1)-receptor antagonist) and norbinaltorpimine (κ-opioid antagonist) respectively, prior to administration of MG (35 mg/kg). The results showed that the antinociceptive effect of MG was not antagonized by AM251; naloxone and naltrindole were effectively blocked; and norbinaltorpimine partially blocked the antinociceptive effect of MG. Naloxonazine did inhibit the effect of MG, but it was not statistically significant. These results demonstrate that CB1 does not directly have a role in the antinociceptive action of MG where the effect was observed with the activation of opioid receptor.

  3. Effects of muscle action type with equal impulse of conditioning activity on postactivation potentiation.

    PubMed

    Bogdanis, Gregory C; Tsoukos, Athanasios; Veligekas, Panagiotis; Tsolakis, Charilaos; Terzis, Gerasimos

    2014-09-01

    This study investigated the effects of muscle action type during conditioning activity (half-squat) on subsequent vertical jump performance. Fourteen track and field athletes (relative half-squat of 2.3 ± 0.3 times their body weight) completed 4 main trials in a randomized and counterbalanced order 5-7 days apart: (a) concentric (CON) half-squats: 7.5 ± 1.2 repetitions against 90% of 1 repetition maximum (1RM), (b) eccentric (ECC) half-squats: 9.3 ± 1.5 repetitions against 70% of 1RM, and (c) 3 sets of 3-second maximal isometric (ISO) half-squats, (d) a control (CTRL) trial, where subjects rested for 10 minutes. The number of repetitions in CON and ECC was adjusted so that the impulse of the vertical ground reaction force was similar to ISO. Countermovement vertical jump (CMJ) performance was evaluated for 21 minutes after each main trial. Countermovement vertical jump performance in ISO was higher than CTRL from the second to the 10th minute of recovery, whereas CMJ performance in ECC was higher than CTRL from the sixth and 10th minute of recovery. Analysis of the peak individual responses revealed an increase in CMJ performance compared with baseline only in ISO (3.0 ± 1.2%; p = 0.045), whereas no significant increases were observed in ECC and CON. Peak CMJ performance for all subjects in ISO and ECC was achieved within 2-10 minutes after the conditioning muscle actions. Isometric were more effective than CON and ECC muscle actions in increasing explosive leg performance when the impulse of the ground reaction force of the conditioning exercise was equated.

  4. A calcium-activated sodium conductance produces a long-duration action potential in the egg of a nemertean worm.

    PubMed Central

    Jaffe, L A; Kado, R T; Kline, D

    1986-01-01

    1. The egg of the nemertean worm Cerebratulus lacteus produced an action potential having a duration of about 9 min. We investigated the ionic conductances which accounted for this long-duration action potential. 2. The peak of the action potential was about +50 mV and depended on extracellular Ca2+, while the plateau potential was about +25 mV and depended on extracellular Na+. 3. Under voltage-clamp conditions, depolarization produced two temporally separate inward currents: a fast current which reached a peak at about 10 ms, and a slow current which took up to 1 min to reach its peak and lasted for several min. 4. The fast current was independent of extracellular Na+, but was blocked by removal of extracellular Ca2+. 5. The slow current was not seen when extracellular Na+ was replaced by choline+ or K+. 6. The slow current did not develop in Ca2+-free sea water, and was reduced to about half if Ca2+ was removed after the current had been initiated. 7. Microinjection of EGTA blocked the slow current, and reduced the action potential duration to about 1 min. 8. We concluded that a voltage-activated Ca2+ conductance produced the peak of the action potential, while a Ca2+-activated Na+ conductance produced its plateau. PMID:2442351

  5. Action myoclonus-renal failure syndrome: diagnostic applications of activity-based probes and lipid analysis

    PubMed Central

    Gaspar, Paulo; Kallemeijn, Wouter W.; Strijland, Anneke; Scheij, Saskia; Van Eijk, Marco; Aten, Jan; Overkleeft, Herman S.; Balreira, Andrea; Zunke, Friederike; Schwake, Michael; Sá Miranda, Clara; Aerts, Johannes M. F. G.

    2014-01-01

    Lysosomal integral membrane protein-2 (LIMP2) mediates trafficking of glucocerebrosidase (GBA) to lysosomes. Deficiency of LIMP2 causes action myoclonus-renal failure syndrome (AMRF). LIMP2-deficient fibroblasts virtually lack GBA like the cells of patients with Gaucher disease (GD), a lysosomal storage disorder caused by mutations in the GBA gene. While GD is characterized by the presence of glucosylceramide-laden macrophages, AMRF patients do not show these. We studied the fate of GBA in relation to LIMP2 deficiency by employing recently designed activity-based probes labeling active GBA molecules. We demonstrate that GBA is almost absent in lysosomes of AMRF fibroblasts. However, white blood cells contain considerable amounts of residual enzyme. Consequently, AMRF patients do not acquire lipid-laden macrophages and do not show increased plasma levels of macrophage markers, such as chitotriosidase, in contrast to GD patients. We next investigated the consequences of LIMP2 deficiency with respect to plasma glycosphingolipid levels. Plasma glucosylceramide concentration was normal in the AMRF patients investigated as well as in LIMP2-deficient mice. However, a marked increase in the sphingoid base, glucosylsphingosine, was observed in AMRF patients and LIMP2-deficient mice. Our results suggest that combined measurements of chitotriosidase and glucosylsphingosine can be used for convenient differential laboratory diagnosis of GD and AMRF. PMID:24212238

  6. Corrective Action Investigation Plan for Corrective Action Unit 104: Area 7 Yucca Flat Atmospheric Test Sites, Nevada National Security Site, Nevada, Revision 0

    SciTech Connect

    Patrick Matthews

    2011-08-01

    CAU 104 comprises the 15 CASs listed below: (1) 07-23-03, Atmospheric Test Site T-7C; (2) 07-23-04, Atmospheric Test Site T7-1; (3) 07-23-05, Atmospheric Test Site; (4) 07-23-06, Atmospheric Test Site T7-5a; (5) 07-23-07, Atmospheric Test Site - Dog (T-S); (6) 07-23-08, Atmospheric Test Site - Baker (T-S); (7) 07-23-09, Atmospheric Test Site - Charlie (T-S); (8) 07-23-10, Atmospheric Test Site - Dixie; (9) 07-23-11, Atmospheric Test Site - Dixie; (10) 07-23-12, Atmospheric Test Site - Charlie (Bus); (11) 07-23-13, Atmospheric Test Site - Baker (Buster); (12) 07-23-14, Atmospheric Test Site - Ruth; (13) 07-23-15, Atmospheric Test Site T7-4; (14) 07-23-16, Atmospheric Test Site B7-b; (15) 07-23-17, Atmospheric Test Site - Climax These sites are being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives (CAAs). Additional information will be obtained by conducting a corrective action investigation before evaluating CAAs and selecting the appropriate corrective action for each CAS. The results of the field investigation will support a defensible evaluation of viable CAAs that will be presented in the Corrective Action Decision Document. The sites will be investigated based on the data quality objectives (DQOs) developed on April 28, 2011, by representatives of the Nevada Division of Environmental Protection and the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office. The DQO process was used to identify and define the type, amount, and quality of data needed to develop and evaluate appropriate corrective actions for CAU 104. The releases at CAU 104 consist of surface-deposited radionuclides from 30 atmospheric nuclear tests. The presence and nature of contamination at CAU 104 will be evaluated based on information collected from a field investigation. Radiological contamination will be evaluated based on a comparison

  7. An in vitro investigation into the cutting action of ultrasonic radicular access preparation instruments.

    PubMed

    Waplington, M; Lumley, P J; Blunt, L

    2000-08-01

    The aim of this study was to investigate the dentine cutting action of a CT4 and SJ4 radicular preparation inserts (Excellence in Endodontics/Analytic Technology, Orange, CA, USA) used in a Piezon Master 400 ultrasonic handpiece (Electro Medical Systems SA, Nyon, Switzerland). Calibration of the ultrasonic inserts was carried out at minimum, medium and maximum power settings using light microscopic measurement of the tip displacement amplitudes (x120 mag). Polished dentine samples were instrumented, at monitored loads, at the three chosen power settings, to produce uniform dentine cuts. The resultant dentine debris was collected for SEM analysis, and comparison with that produced by rotary instruments. The depth of dentine cut was measured at x30 magnification. Fracture of the SJ4 insert was noted at medium power setting. Results revealed a significant increase in both displacement amplitude and depth of dentine cut with power setting (ANOVA P < 0.001). Dentine chip size increased with power, and differed in structure and appearance from those produced by rotary instruments. In conclusion, ultrasonic cutting is influenced significantly by power setting, and dentine chip formation is fundamentally different to rotary instrumentation.

  8. Record of Technical Change {number_sign}2 for ''Corrective Action Investigation Plan for Corrective Action Unit 261: Test Cell A Leachfield System, Nevada Test Site, Nevada,'' Revision 0

    SciTech Connect

    US DOE Nevada Operations Office

    2000-06-08

    This Record of Technical Change updates the technical information included in ''Corrective Action Investigation Plan for Corrective Action Unit 261: Test Cell A Leachfield System, Nevada Test Site, Nevada,'' Revision 0, DOE/NV--515.

  9. Record of Technical Change {number_sign}1 to ''Corrective Action Investigation Plan for Corrective Action Unit 261: Test Cell A Leachfield System, Nevada Test Site, Nevada,'' Revision 0, DOE/NV-519

    SciTech Connect

    US DOE Nevada Operations Office

    2000-02-25

    This Record of Technical Change provides updates to the technical information included in ''Corrective Action Investigation Plan for Corrective Action Unit 261: Test Cell A Leachfield System, Nevada Test Site, Nevada,'' DOE/NV--519.

  10. Computational investigations on polymerase actions in gene transcription and replication: Combining physical modeling and atomistic simulations

    NASA Astrophysics Data System (ADS)

    Jin, Yu

    2016-01-01

    Polymerases are protein enzymes that move along nucleic acid chains and catalyze template-based polymerization reactions during gene transcription and replication. The polymerases also substantially improve transcription or replication fidelity through the non-equilibrium enzymatic cycles. We briefly review computational efforts that have been made toward understanding mechano-chemical coupling and fidelity control mechanisms of the polymerase elongation. The polymerases are regarded as molecular information motors during the elongation process. It requires a full spectrum of computational approaches from multiple time and length scales to understand the full polymerase functional cycle. We stay away from quantum mechanics based approaches to the polymerase catalysis due to abundant former surveys, while addressing statistical physics modeling approaches along with all-atom molecular dynamics simulation studies. We organize this review around our own modeling and simulation practices on a single subunit T7 RNA polymerase, and summarize commensurate studies on structurally similar DNA polymerases as well. For multi-subunit RNA polymerases that have been actively studied in recent years, we leave systematical reviews of the simulation achievements to latest computational chemistry surveys, while covering only representative studies published very recently, including our own work modeling structure-based elongation kinetic of yeast RNA polymerase II. In the end, we briefly go through physical modeling on elongation pauses and backtracking activities of the multi-subunit RNAPs. We emphasize on the fluctuation and control mechanisms of the polymerase actions, highlight the non-equilibrium nature of the operation system, and try to build some perspectives toward understanding the polymerase impacts from the single molecule level to a genome-wide scale. Project supported by the National Natural Science Foundation (Grant No. 11275022).

  11. Exploring the Utility of Action Research to Investigate Second-Language Classrooms as Complex Systems

    ERIC Educational Resources Information Center

    Ahmadian, Mohammad Javad; Tavakoli, Mansoor

    2011-01-01

    Action research is geared to changes for the better and has the potential to assist teachers to extend their teaching skills and develop a deeper understanding of themselves, their classroom and their learners. However, in the area of applied linguistics, the viability of action research has been seriously questioned. In this article, we argue…

  12. Development of an in vitro Hepatocyte Model to Investigate Chemical Mode of Action

    EPA Science Inventory

    There is a clear need to identify and characterize the potential of liver in vitro models that can be used to replace animals for mode of action analysis. Our goal is to use in vitro models for mode of action prediction which recapitulate critical cellular processes underlying in...

  13. An Investigation of Preschool Teachers' Ways of Seeing Action Research Using Phenomenography

    ERIC Educational Resources Information Center

    Lam, Ho Cheong

    2016-01-01

    One of the purposes of introducing the use of action research in schools is to bring about educational change in policy and practice. To make this happen, it is of paramount importance to look into how teachers actually see action research. We, the writers of this paper, are teacher educators, teaching preschool teachers courses related to action…

  14. 4-Alkynylphenylsilatranes: Insecticidal activity, mammalian toxicity, and mode of action

    SciTech Connect

    Horsham, M.A.; Palmer, C.J.; Cole, L.M.; Casida, J.E. )

    1990-08-01

    4-Ethynyl- and 4-(prop-1-ynyl)phenylsilatranes (N(CH{sub 2}CH{sub 2}O){sub 3}SiR, R = C{sub 6}H{sub 4}-4-C{triple bond}CH or C{sub 6}H{sub 4}-4-C{triple bond}CCH{sub 3}) are highly toxic to houseflies (pretreated with piperonyl butoxide) and milkweed bugs (topical LD{sub 50}s 3-14 {mu}g/g) and to mice (intraperitoneal LD{sub 50}s 0.4-0.9 mg/kg), and they are moderately potent inhibitors of the ({sup 35}S)-tert-butylbicyclophosphorothionate or TBPS binding site (GABA-gated chloride channel) of mouse brain membranes. Scatchard analysis indicates noncompetitive interaction of 4-ethynylphenylsilatrane with the TBPS binding site. Phenylsilatrane analogues with 4-substituents of H, CH{sub 3}, Cl, Br, and C{triple bond}CSi(CH{sub 3}){sub 3} are highly toxic to mice but have little or no activity in the insect and receptor assays. Radioligand binding studies with (4-{sup 3}H)phenylsilatrane failed to reveal a specific binding site in mouse brain. Silatranes with R = H, CH{sub 3}, CH{sub 2}Cl, CH{double bond}CH{sub 2}, OCH{sub 2}CH{sub 3}, and C{sub 6}H{sub 4}-4-CH{sub 2}CH{sub 3} are of little or no activity in the insect and mouse toxicity and TBPS binding site assays as are the trithia and monocyclic analogues of phenylsilatrane. 4-Alkynylphenylsilatranes are new probes to examine the GABA receptor-ionophore complex of insects and mammals.

  15. Activity of human motor system during action observation is modulated by object presence.

    PubMed

    Villiger, Michael; Chandrasekharan, Sanjay; Welsh, Timothy N

    2011-03-01

    Neurons in the monkey mirror neuron system (MNS) become active when actions are observed or executed. Increases in activity are greater when objects are engaged than when the actions are mimed. This modulation occurs even when object manipulation is hidden from view. We examined whether human motor systems are similarly modulated during action observation because such observation-related modulations are potentially mediated by a putative human MNS. Transcranial magnetic stimulation (TMS) was used to elicit motor-evoked potentials (MEPs) of a grasping muscle while participants observed actual or pantomimed grasping movements whose endpoints were sometimes hidden from view. MEP amplitudes were found to be modulated by object presence. Critically, the object-based modulation was found when the participant directly observed object manipulation and when the object manipulation had to be inferred because it was hidden. These findings parallel studies of MNS activity in monkeys and support the hypothesis that the MNS influences motor system activity during action observation. Although the object-based modulation of MEP amplitudes was consistent with the hypotheses, the direction of the modulation was not--MEP amplitudes decreased during action observation in contrast to the increase that has previously been observed. We suggest that the decrease in MEP amplitude on object-present trials resulted from inhibitory mechanisms that were activated to suppress the observation-evoked response codes from generating overt muscle activity.

  16. Synthesis, antitumor activity, and mechanism of action of 6-acrylic phenethyl ester-2-pyranone derivatives.

    PubMed

    Fang, Sai; Chen, Lei; Yu, Miao; Cheng, Bao; Lin, Yongsheng; Morris-Natschke, Susan L; Lee, Kuo-Hsiung; Gu, Qiong; Xu, Jun

    2015-04-28

    Based on the scaffolds of caffeic acid phenethyl ester (CAPE) as well as bioactive lactone-containing compounds, 6-acrylic phenethyl ester-2-pyranone derivatives were synthesized and evaluated against five tumor cell lines (HeLa, C6, MCF-7, A549, and HSC-2). Most of the new derivatives exhibited moderate to potent cytotoxic activity. Moreover, HeLa cell lines showed higher sensitivity to these compounds. In particular, compound showed potent cytotoxic activity (IC50 = 0.50-3.45 μM) against the five cell lines. Further investigation on the mechanism of action showed that induced apoptosis, arrested the cell cycle at G2/M phases in HeLa cells, and inhibited migration through disruption of the actin cytoskeleton. In addition, ADMET properties were also calculated in silico, and compound showed good ADMET properties with good absorption, low hepatotoxicity, and good solubility, and thus, could easily be bound to carrier proteins, without inhibition of CYP2D6. A structure-activity relationship (SAR) analysis indicated that compounds with ortho-substitution on the benzene ring exhibited obviously increased cytotoxic potency. This study indicated that compound is a promising compound as an antitumor agent. PMID:25800703

  17. Diversity, Antimicrobial Action and Structure-Activity Relationship of Buffalo Cathelicidins.

    PubMed

    Brahma, Biswajit; Patra, Mahesh Chandra; Karri, Satyanagalakshmi; Chopra, Meenu; Mishra, Purusottam; De, Bidhan Chandra; Kumar, Sushil; Mahanty, Sourav; Thakur, Kiran; Poluri, Krishna Mohan; Datta, Tirtha Kumar; De, Sachinandan

    2015-01-01

    Cathelicidins are an ancient class of antimicrobial peptides (AMPs) with broad spectrum bactericidal activities. In this study, we investigated the diversity and biological activity of cathelicidins of buffalo, a species known for its disease resistance. A series of new homologs of cathelicidin4 (CATHL4), which were structurally diverse in their antimicrobial domain, was identified in buffalo. AMPs of newly identified buffalo CATHL4s (buCATHL4s) displayed potent antimicrobial activity against selected Gram positive (G+) and Gram negative (G-) bacteria. These peptides were prompt to disrupt the membrane integrity of bacteria and induced specific changes such as blebing, budding, and pore like structure formation on bacterial membrane. The peptides assumed different secondary structure conformations in aqueous and membrane-mimicking environments. Simulation studies suggested that the amphipathic design of buCATHL4 was crucial for water permeation following membrane disruption. A great diversity, broad-spectrum antimicrobial action, and ability to induce an inflammatory response indicated the pleiotropic role of cathelicidins in innate immunity of buffalo. This study suggests short buffalo cathelicidin peptides with potent bactericidal properties and low cytotoxicity have potential translational applications for the development of novel antibiotics and antimicrobial peptidomimetics.

  18. Diversity, Antimicrobial Action and Structure-Activity Relationship of Buffalo Cathelicidins.

    PubMed

    Brahma, Biswajit; Patra, Mahesh Chandra; Karri, Satyanagalakshmi; Chopra, Meenu; Mishra, Purusottam; De, Bidhan Chandra; Kumar, Sushil; Mahanty, Sourav; Thakur, Kiran; Poluri, Krishna Mohan; Datta, Tirtha Kumar; De, Sachinandan

    2015-01-01

    Cathelicidins are an ancient class of antimicrobial peptides (AMPs) with broad spectrum bactericidal activities. In this study, we investigated the diversity and biological activity of cathelicidins of buffalo, a species known for its disease resistance. A series of new homologs of cathelicidin4 (CATHL4), which were structurally diverse in their antimicrobial domain, was identified in buffalo. AMPs of newly identified buffalo CATHL4s (buCATHL4s) displayed potent antimicrobial activity against selected Gram positive (G+) and Gram negative (G-) bacteria. These peptides were prompt to disrupt the membrane integrity of bacteria and induced specific changes such as blebing, budding, and pore like structure formation on bacterial membrane. The peptides assumed different secondary structure conformations in aqueous and membrane-mimicking environments. Simulation studies suggested that the amphipathic design of buCATHL4 was crucial for water permeation following membrane disruption. A great diversity, broad-spectrum antimicrobial action, and ability to induce an inflammatory response indicated the pleiotropic role of cathelicidins in innate immunity of buffalo. This study suggests short buffalo cathelicidin peptides with potent bactericidal properties and low cytotoxicity have potential translational applications for the development of novel antibiotics and antimicrobial peptidomimetics. PMID:26675301

  19. Synthesis, Antitumor Activity, and Mechanism of Action of 6-Acrylic Phenethyl Ester-2-pyranone Derivatives

    PubMed Central

    Fang, Sai; Chen, Lei; Yu, Miao; Cheng, Bao; Lin, Yongsheng; Morris-Natschke, Susan L.; Lee, Kuo-Hsiung; Gu, Qiong; Xu, Jun

    2015-01-01

    Based on the scaffolds of caffeic acid phenethyl ester (CAPE) as well as bioactive lactone-containing compounds, 6-acrylic phenethyl ester-2-pyranone derivatives were synthesized and evaluated against five tumor cell lines (HeLa, C6, MCF-7, A549, and HSC-2). Most of the new derivatives exhibited moderate to potent cytotoxic activity. Moreover, HeLa cell lines showed higher sensitivity to these compounds. Particularly, compound 5o showed potent cytotoxic activity (IC50 = 0.50 – 3.45 μM) against the five cell lines. Further investigation on the mechanism of action showed that 5o induced apoptosis, arrested the cell cycle at G2/M phases in HeLa cells, and inhibited migration through disruption of the actin cytoskeleton. In addition, ADME properties were also calculated in silico, and compound 5o showed good ADMET properties with good absorption, low hepatotoxicity, and good solubility, and thus, could easily be bound to carrier proteins, without inhibition of CYP2D6. A structure-activity relationship (SAR) analysis indicated that compounds with ortho-substitution on the benzene ring exhibited obviously increased cytotoxic potency. This study indicated that compound 5o is a promising compound as an antitumor agent. PMID:25800703

  20. Diversity, Antimicrobial Action and Structure-Activity Relationship of Buffalo Cathelicidins

    PubMed Central

    Brahma, Biswajit; Patra, Mahesh Chandra; Karri, Satyanagalakshmi; Chopra, Meenu; Mishra, Purusottam; De, Bidhan Chandra; Kumar, Sushil; Mahanty, Sourav; Thakur, Kiran; Poluri, Krishna Mohan; Datta, Tirtha Kumar; De, Sachinandan

    2015-01-01

    Cathelicidins are an ancient class of antimicrobial peptides (AMPs) with broad spectrum bactericidal activities. In this study, we investigated the diversity and biological activity of cathelicidins of buffalo, a species known for its disease resistance. A series of new homologs of cathelicidin4 (CATHL4), which were structurally diverse in their antimicrobial domain, was identified in buffalo. AMPs of newly identified buffalo CATHL4s (buCATHL4s) displayed potent antimicrobial activity against selected Gram positive (G+) and Gram negative (G-) bacteria. These peptides were prompt to disrupt the membrane integrity of bacteria and induced specific changes such as blebing, budding, and pore like structure formation on bacterial membrane. The peptides assumed different secondary structure conformations in aqueous and membrane-mimicking environments. Simulation studies suggested that the amphipathic design of buCATHL4 was crucial for water permeation following membrane disruption. A great diversity, broad-spectrum antimicrobial action, and ability to induce an inflammatory response indicated the pleiotropic role of cathelicidins in innate immunity of buffalo. This study suggests short buffalo cathelicidin peptides with potent bactericidal properties and low cytotoxicity have potential translational applications for the development of novel antibiotics and antimicrobial peptidomimetics. PMID:26675301

  1. Synthesis and biological activities of d-chiro-inositol analogues with insulin-like actions.

    PubMed

    Rendle, P M; Kassibawi, F; Johnston, K A; Hart, J B; Cameron, S A; Falshaw, A; Painter, G F; Loomes, K M

    2016-10-21

    d-chiro-inositol (DCI, 1) evokes therapeutic actions in diabetes and insulin resistance but has sub-optimal pharmacokinetic profiles. To investigate what positions on the DCI cyclohexanol ring may be amenable to modification to improve pharmaceutical formulations, a series of analogues based on DCI were synthesised. These compounds were then evaluated for their ability to stimulate glucose transport using 3T3-L1 adipocytes as a model system. Positional analyses indicate that the hydroxyl group at position 1 is not essential for activity and can be modified without affecting glucose uptake. Removal of the hydroxyl at position 3 also had minimal effect on activity but this group is sensitive to modification. By comparison, the oxygen at position 2 is crucial to the potency of DCI, although this group can withstand modification without fundamentally affecting activity. These data reveal that positions 1 and 2 on the cyclohexanol ring of DCI offer further scope for modification to develop DCI analogues with desirable pharmacokinetic profiles for the potential treatment of metabolic disease. PMID:27410479

  2. Action planning as predictor of health protective and health risk behavior: an investigation of fruit and snack consumption

    PubMed Central

    van Osch, Liesbeth; Beenackers, Mariëlle; Reubsaet, Astrid; Lechner, Lilian; Candel, Math; de Vries, Hein

    2009-01-01

    Background Large discrepancies between people's intention to eat a healthy diet and actual dietary behavior indicate that motivation is not a sufficient instigator for healthy behavior. Research efforts to decrease this 'intention - behavior gap' have centered on aspects of self-regulation, most importantly self-regulatory planning. Most studies on the impact of self-regulatory planning in health and dietary behavior focus on the promotion of health protective behaviors. This study investigates and compares the predictive value of action planning in health protective behavior and the restriction of health risk behavior. Methods Two longitudinal observational studies were performed simultaneously, one focusing on fruit consumption (N = 572) and one on high-caloric snack consumption (N = 585) in Dutch adults. Structural equation modeling was used to investigate and compare the predictive value of action planning in both behaviors, correcting for demographics and the influence of motivational factors and past behavior. The nature of the influence of action planning was investigated by testing mediating and moderating effects. Results Action planning was a significant predictor of fruit consumption and restricted snack consumption beyond the influence of motivational factors and past behavior. The strength of the predictive value of action planning did not differ between the two behaviors. Evidence for mediation of the intention - behavior relationship was found for both behaviors. Positive moderating effects of action planning were demonstrated for fruit consumption, indicating that individuals who report high levels of action planning are significantly more likely to translate their intentions into actual behavior. Conclusion The results indicate that the planning of specific preparatory actions predicts the performance of healthy dietary behavior and support the application of self-regulatory planning in both health protective and health risk behaviors. Future

  3. Increased Event-Related Potentials and Alpha-, Beta-, and Gamma-Activity Associated with Intentional Actions

    PubMed Central

    Karch, Susanne; Loy, Fabian; Krause, Daniela; Schwarz, Sandra; Kiesewetter, Jan; Segmiller, Felix; Chrobok, Agnieszka I.; Keeser, Daniel; Pogarell, Oliver

    2016-01-01

    Objective: Internally guided actions are defined as being purposeful, self-generated and offering choices between alternatives. Intentional actions are essential to reach individual goals. In previous empirical studies, internally guided actions were predominantly related to functional responses in frontal and parietal areas. The aim of the present study was to distinguish event-related potentials and oscillatory responses of intentional actions and externally guided actions. In addition, we compared neurobiological findings of the decision which action to perform with those referring to the decision whether or not to perform an action. Methods: Twenty-eight subjects participated in adapted go/nogo paradigms, including a voluntary selection condition allowing participants to (1) freely decide whether to press the response button or (2) to decide whether they wanted to press the response button with the right index finger or the left index finger. Results: The reaction times were increased when participants freely decided whether and how they wanted to respond compared to the go condition. Intentional processes were associated with a fronto-centrally located N2 and P3 potential. N2 and P3 amplitudes were increased during intentional actions compared to instructed responses (go). In addition, increased activity in the alpha-, beta- and gamma-frequency range was shown during voluntary behavior rather than during externally guided responses. Conclusion: These results may indicate that an additional cognitive process is needed for intentional actions compared to instructed behavior. However, the neural responses were comparatively independent of the kind of decision that was made (1) decision which action to perform; (2) decision whether or not to perform an action). Significance: The study demonstrates the importance of fronto-central alpha-, beta-, and gamma oscillations for voluntary behavior. PMID:26834680

  4. Corrective Action Investigation Plan for Corrective Action Unit 230: Area 22 Sewage Lagoons and Corrective Action Unit 320: Area 22 Desert Rock Airport Strainer Box, Nevada Test Site, Nevada

    SciTech Connect

    US DOE /Nevada Operations Office

    1999-06-10

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, Nevada Operation Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 230/320 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 230 consists of Corrective Action Site (CAS) 22-03-01, Sewage Lagoon; while CAU 320 consists of CAS 22-99-01, Strainer Box. These CAUs are referred to as CAU 230/320 or the Sewage Lagoons Site. The Sewage Lagoons Site also includes an Imhoff tank, sludge bed, and associated buried sewer piping. Located in Area 22, the site was used between 1951 to 1958 for disposal of sanitary sewage effluent from the historic Camp Desert Rock Facility at the Nevada Test Site in Nevada. Based on site history, the contaminants of potential concern include volatile organic compounds (VOCs), semivolatile organic compounds, total petroleum hydrocarbons (TPH), and radionuclides. Vertical migration is estimated to be less than 12 feet below ground surface, and lateral migration is limited to the soil immediately adjacent to or within areas of concern. The proposed investigation will involve a combination of field screening for VOCs and TPH using the direct-push method and excavation using a backhoe to gather soil samples for analysis. Gamma spectroscopy will also be conducted for waste management purposes. Sampling locations will be biased to suspected worst-case areas including the nearby sludge bed, sewage lagoon inlet(s) and outlet(s), disturbed soil surrounding the lagoons, surface drainage channel south of the lagoons, and the area near the Imhoff tank. The results of this field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  5. Corrective Action Investigation Plan for Corrective Action Unit 230: Area 22 Sewage Lagoons and Corrective Action Unit 320: Area 22 Desert Rock Air port Strainer Box, Nevada Test Site, Nevada

    SciTech Connect

    U.S. Department of Energy, Nevada Operations Office

    1999-06-10

    This Corrective Action Investigation Plan contains the US Department of Energy, Nevada Operation Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 230/320 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 230 consists of Corrective Action Site (CAS) 22-03-01, Sewage Lagoon; while CAU 320 consists of CAS 22-99-01, Strainer Box. These CAUs are referred to as CAU 230/320 or the Sewage Lagoons Site. The Sewage Lagoons Site also includes an Imhoff tank, sludge bed, and associated buried sewer piping. Located in Area 22, the site was used between 1951 to 1958 for disposal of sanitary sewage effluent from the historic Camp Desert Rock Facility at the Nevada Test Site in Nevada. Based on site history, the contaminants of potential concern include volatile organic compounds (VOCs), semivolatile organic compounds, total petroleum hydrocarbons (TPH), and radionuclides. Vertical migration is estimated to be less than 12 feet below ground surface, and lateral migration is limited to the soil immediately adjacent to or within areas of concern. The proposed investigation will involve a combination of field screening for VOCs and TPH using the direct-push method and excavation using a backhoe to gather soil samples for analysis. Gamma spectroscopy will also be conducted for waste management purposes. Sampling locations will be biased to suspected worst-case areas including the nearby sludge bed, sewage lagoon inlet(s) and outlet(s), disturbed soil surrounding the lagoons, surface drainage channel south of the lagoons, and the area near the Imhoff tank. The results of this field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  6. Light Activated Serotonin for Exploring Its Action in Biological Systems

    PubMed Central

    Rea, Adam C.; Vandenberg, Laura N.; Ball, Rebecca E.; Snouffer, Ashley A.; Hudson, Alicia G.; Zhu, Yue; McLain, Duncan E.; Johnston, Lindsey L.; Lauderdale, James D.; Levin, Michael; Dore, Timothy M.

    2013-01-01

    Summary Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. To explore the role of 5-HT in a variety of physiological contexts, we have created two forms of “caged” 5-HT, BHQ-O-5HT and BHQ-N-5HT. When exposed to 365- or 740-nm light, BHQ-O-5HT releases 5-HT through 1- or 2-photon excitation, respectively. BHQ-O-5HT mediated changes in neural activity in cultured primary sensory neurons from mouse and the trigeminal ganglion and optic tectum of intact zebrafish larvae in the form of high amplitude spiking in response to light. In Xenopus laevis embryos, 5-HT released from BHQ-O-5HT upon exposure to light increased the occurrence of LR patterning defects. Maximal rates of LR defects were observed when 5-HT was released at stage 5 compared to stage 8. These experiments show the potential for BHQ-caged serotonins in studying 5-HT-regulated physiological processes. PMID:24333002

  7. The Austrian radon activities on the way to the national radon action plan.

    PubMed

    Gruber, V; Ringer, W; Wurm, G; Haider, W

    2014-07-01

    Based on the new Euratom Basic Safety Standards (BSS), all EU member states will be obliged to design a strategy to address long-term risks from radon exposure, which is laid down in the 'national radon action plan'. In Austria, the National Radon Centre is responsible for the development of the action plan. This paper presents the current and planned radon protection activities on the way to establish the radon action plan--like the national radon database, the definition of radon risk areas by improving the existing radon map, as well as strategies and activities to increase the radon awareness of the public and decision-makers and to involve the building sector. The impact of and the need for actions caused by the BSS requirements on the Austrian radon legislation, strategy and programme are discussed.

  8. Corrective Action Investigation Plan for Corrective Action Unit 545: Dumps, Waste Disposal Sites, and Buried Radioactive Materials Nevada Test Site, Nevada, Revision 0

    SciTech Connect

    Wickline, Alfred

    2007-06-01

    Corrective Action Unit 545, Dumps, Waste Disposal Sites, and Buried Radioactive Materials, consists of seven inactive sites located in the Yucca Flat area and one inactive site in the Pahute Mesa area. The eight CAU 545 sites consist of craters used for mud disposal, surface or buried waste disposed within craters or potential crater areas, and sites where surface or buried waste was disposed. The CAU 545 sites were used to support nuclear testing conducted in the Yucca Flat area during the 1950s through the early 1990s, and in Area 20 in the mid-1970s. This Corrective Action Investigation Plan has been developed in accordance with the Federal Facility Agreement and Consent Order that was agreed to by the State of Nevada, the U.S. Department of Energy, and the U.S. Department of Defense. Under the Federal Facility Agreement and Consent Order, this Corrective Action Investigation Plan will be submitted to the Nevada Division of Environmental Protection for approval. Fieldwork will be conducted following approval.

  9. Dialogue--Missing in Action Competence: A Cultural Historical Activity Theory Approach in a Botswana School

    ERIC Educational Resources Information Center

    Silo, Nthalivi

    2013-01-01

    An in-depth case study on children's participation in environmental management activities in a primary school in Botswana was undertaken, drawing on cultural historical activity theory (CHAT) and the action competence model. This research revealed that due to a lack of dialogue between teachers and children, teachers tended to view children's…

  10. Contextual action recognition and target localization with an active allocation of attention on a humanoid robot.

    PubMed

    Ognibene, Dimitri; Chinellato, Eris; Sarabia, Miguel; Demiris, Yiannis

    2013-09-01

    Exploratory gaze movements are fundamental for gathering the most relevant information regarding the partner during social interactions. Inspired by the cognitive mechanisms underlying human social behaviour, we have designed and implemented a system for a dynamic attention allocation which is able to actively control gaze movements during a visual action recognition task exploiting its own action execution predictions. Our humanoid robot is able, during the observation of a partner's reaching movement, to contextually estimate the goal position of the partner's hand and the location in space of the candidate targets. This is done while actively gazing around the environment, with the purpose of optimizing the gathering of information relevant for the task. Experimental results on a simulated environment show that active gaze control, based on the internal simulation of actions, provides a relevant advantage with respect to other action perception approaches, both in terms of estimation precision and of time required to recognize an action. Moreover, our model reproduces and extends some experimental results on human attention during an action perception.

  11. Elucidation of the Mode of Action of a New Antibacterial Compound Active against Staphylococcus aureus and Pseudomonas aeruginosa

    PubMed Central

    Gerits, Evelien; Blommaert, Eline; Lippell, Anna; O’Neill, Alex J.; Weytjens, Bram; De Maeyer, Dries; Fierro, Ana Carolina; Marchal, Kathleen; Marchand, Arnaud; Chaltin, Patrick; Spincemaille, Pieter; De Brucker, Katrijn; Thevissen, Karin; Cammue, Bruno P. A.; Swings, Toon; Liebens, Veerle; Fauvart, Maarten; Verstraeten, Natalie; Michiels, Jan

    2016-01-01

    Nosocomial and community-acquired infections caused by multidrug resistant bacteria represent a major human health problem. Thus, there is an urgent need for the development of antibiotics with new modes of action. In this study, we investigated the antibacterial characteristics and mode of action of a new antimicrobial compound, SPI031 (N-alkylated 3, 6-dihalogenocarbazol 1-(sec-butylamino)-3-(3,6-dichloro-9H-carbazol-9-yl)propan-2-ol), which was previously identified in our group. This compound exhibits broad-spectrum antibacterial activity, including activity against the human pathogens Staphylococcus aureus and Pseudomonas aeruginosa. We found that SPI031 has rapid bactericidal activity (7-log reduction within 30 min at 4x MIC) and that the frequency of resistance development against SPI031 is low. To elucidate the mode of action of SPI031, we performed a macromolecular synthesis assay, which showed that SPI031 causes non-specific inhibition of macromolecular biosynthesis pathways. Liposome leakage and membrane permeability studies revealed that SPI031 rapidly exerts membrane damage, which is likely the primary cause of its antibacterial activity. These findings were supported by a mutational analysis of SPI031-resistant mutants, a transcriptome analysis and the identification of transposon mutants with altered sensitivity to the compound. In conclusion, our results show that SPI031 exerts its antimicrobial activity by causing membrane damage, making it an interesting starting point for the development of new antibacterial therapies. PMID:27167126

  12. Elucidation of the Mode of Action of a New Antibacterial Compound Active against Staphylococcus aureus and Pseudomonas aeruginosa.

    PubMed

    Gerits, Evelien; Blommaert, Eline; Lippell, Anna; O'Neill, Alex J; Weytjens, Bram; De Maeyer, Dries; Fierro, Ana Carolina; Marchal, Kathleen; Marchand, Arnaud; Chaltin, Patrick; Spincemaille, Pieter; De Brucker, Katrijn; Thevissen, Karin; Cammue, Bruno P A; Swings, Toon; Liebens, Veerle; Fauvart, Maarten; Verstraeten, Natalie; Michiels, Jan

    2016-01-01

    Nosocomial and community-acquired infections caused by multidrug resistant bacteria represent a major human health problem. Thus, there is an urgent need for the development of antibiotics with new modes of action. In this study, we investigated the antibacterial characteristics and mode of action of a new antimicrobial compound, SPI031 (N-alkylated 3, 6-dihalogenocarbazol 1-(sec-butylamino)-3-(3,6-dichloro-9H-carbazol-9-yl)propan-2-ol), which was previously identified in our group. This compound exhibits broad-spectrum antibacterial activity, including activity against the human pathogens Staphylococcus aureus and Pseudomonas aeruginosa. We found that SPI031 has rapid bactericidal activity (7-log reduction within 30 min at 4x MIC) and that the frequency of resistance development against SPI031 is low. To elucidate the mode of action of SPI031, we performed a macromolecular synthesis assay, which showed that SPI031 causes non-specific inhibition of macromolecular biosynthesis pathways. Liposome leakage and membrane permeability studies revealed that SPI031 rapidly exerts membrane damage, which is likely the primary cause of its antibacterial activity. These findings were supported by a mutational analysis of SPI031-resistant mutants, a transcriptome analysis and the identification of transposon mutants with altered sensitivity to the compound. In conclusion, our results show that SPI031 exerts its antimicrobial activity by causing membrane damage, making it an interesting starting point for the development of new antibacterial therapies. PMID:27167126

  13. In vitro investigations on the mode of action of the hydroxypyridone antimycotics rilopirox and piroctone on Candida albicans.

    PubMed

    Sigle, H C; Schäfer-Korting, M; Korting, H C; Hube, B; Niewerth, M

    2006-05-01

    Rilopirox and piroctone belong to the class of hydroxypyridone antimycotics. This class is not related to other antimycotics. In contrast to azole antimycotics and polyene antimycotics the mode of action of hydroxypyridone antimycotics is not fully understood. Inhibition of cellular uptake of essential compounds as well as loss of other compounds seems to be only a secondary effect of a primary not known action of these drugs. The antifungal effect in vitro depends on the medium used. The hyphal induction of Candida albicans is inhibited by hydroxypyridone antimycotics, but this effect is compensated by iron ions. A damage of the cell membrane and a direct influence on adenosine triphosphate synthesis, respectively, do not seem to be part of the mode of action. But there are clear hints that reactive oxygen species (ROS) and available metabolic activity are important parts of the mode of action of the hydroxypyridone antimycotics rilopirox and piroctone. PMID:16681805

  14. Investigating stability using nonlinear quasihomogeneous approximation to differential equations with impulsive action

    SciTech Connect

    Dvirny, A. I.; Slyn'ko, V. I. E-mail: vitstab@ukr.net

    2014-06-01

    Inverse theorems to Lyapunov's direct method are established for quasihomogeneous systems of differential equations with impulsive action. Conditions for the existence of Lyapunov functions satisfying typical bounds for quasihomogeneous functions are obtained. Using these results, we establish conditions for an equilibrium of a nonlinear system with impulsive action to be stable, using the properties of a quasihomogeneous approximation to the system. The results are illustrated by an example of a large-scale system with homogeneous subsystems. Bibliography: 30 titles. (paper)

  15. Teacher Organizations and Collective Action: A Review of History and a Survey of School District Activity, 1964-65. Volume I, Collective Action by Public School Teachers. Final Report.

    ERIC Educational Resources Information Center

    Wildman, Wesley A.; Burns, Robert K.

    This first of 4 volumes comprising a broad investigation of teacher collective action in local school districts focuses on the history of such activity. Part I (42 pages) of this volume traces the history of the major teacher organizations--local, state, and national--from 1857 to 1967 with respect to their concern for teacher welfare and their…

  16. Event-related potentials reveal early activation of body part representations in action concept comprehension.

    PubMed

    Lu, Aitao; Liu, Jing; Zhang, John X

    2012-03-01

    With tasks involving action concept comprehension, many fMRI studies have reported brain activations in sensori-motor regions specific to effectors of the referent action. There is relatively less evidence whether such activations reflect early semantic access or late conceptual re-processing. Here we recorded event-related potentials when participants recognized noun-verb pairs. For Congruent pairs, the verb was the one most commonly associated with the noun (e.g., football-kick). Compared with a control condition, verbs in Congruent pairs showed priming effects in the time windows of 100-150 ms and 210-260 ms. Such activation seems to be specific to body part but not other aspects of the action as similar priming effect was also found when the noun and verb involved different actions though sharing the same body part (e.g., football-jump), documenting for the first time the early activation of body part representations in action concept comprehension. PMID:22306088

  17. Event-related potentials reveal early activation of body part representations in action concept comprehension.

    PubMed

    Lu, Aitao; Liu, Jing; Zhang, John X

    2012-03-01

    With tasks involving action concept comprehension, many fMRI studies have reported brain activations in sensori-motor regions specific to effectors of the referent action. There is relatively less evidence whether such activations reflect early semantic access or late conceptual re-processing. Here we recorded event-related potentials when participants recognized noun-verb pairs. For Congruent pairs, the verb was the one most commonly associated with the noun (e.g., football-kick). Compared with a control condition, verbs in Congruent pairs showed priming effects in the time windows of 100-150 ms and 210-260 ms. Such activation seems to be specific to body part but not other aspects of the action as similar priming effect was also found when the noun and verb involved different actions though sharing the same body part (e.g., football-jump), documenting for the first time the early activation of body part representations in action concept comprehension.

  18. Conscientiousness, extroversion, and action control: comparing moderate and vigorous physical activity.

    PubMed

    de Bruijn, Gert-Jan; de Groot, Ruben; van den Putte, Bas; Rhodes, Ryan

    2009-12-01

    The present study explored the influence of the Big Five dimensions extroversion and conscientiousness on action control regarding both moderate and vigorous physical activity within the framework of the theory of planned behavior (TPB). Prospective data were available from 186 respondents, who completed measures of intention, cognitive and affective attitude, subjective norm, perceived behavioral control, extroversion, conscientiousness, and physical activity at T1. Four weeks later, physical activity was assessed again. Respondents were grouped into four profiles: nonintenders, successful nonintenders, unsuccessful intenders, and successful intenders. Logistic regression analyses revealed that successful enactment in moderate physical activity was associated with extroversion, subjective norm, and affective attitude, whereas successful enactment in vigorous physical activity was associated with conscientiousness. Findings illustrate the differential role played by personality dimensions and TPB concepts in the explanation of moderate and vigorous physical activity action control.

  19. Basal Ganglia Activity Mirrors a Benefit of Action and Reward on Long-Lasting Event Memory

    PubMed Central

    Koster, Raphael; Guitart-Masip, Marc; Dolan, Raymond J.; Düzel, Emrah

    2015-01-01

    The expectation of reward is known to enhance a consolidation of long-term memory for events. We tested whether this effect is driven by positive valence or action requirements tied to expected reward. Using a functional magnetic resonance imaging (fMRI) paradigm in young adults, novel images predicted gain or loss outcomes, which in turn were either obtained or avoided by action or inaction. After 24 h, memory for these images reflected a benefit of action as well as a congruence of action requirements and valence, namely, action for reward and inaction for avoidance. fMRI responses in the hippocampus, a region known to be critical for long-term memory function, reflected the anticipation of inaction. In contrast, activity in the putamen mirrored the congruence of action requirement and valence, whereas other basal ganglia regions mirrored overall action benefits on long-lasting memory. The findings indicate a novel type of functional division between the hippocampus and the basal ganglia in the motivational regulation of long-term memory consolidation, which favors remembering events that are worth acting for. PMID:26420783

  20. Patterns of brain activity distinguishing free and forced actions: contribution from sensory cortices

    PubMed Central

    Kostelecki, Wojciech; Mei, Ye; Garcia Dominguez, Luis; Pérez Velázquez, José L.

    2012-01-01

    The neural basis of decision-making is extremely complex due to the large number of factors that contribute to the outcome of even the most basic actions as well as the range of appropriate responses within many behavioral contexts. To better understand the neural processes underlying basic forms of decision-making, this study utilized an experiment that required a choice about whether to press a button with the right or left hand. These instances of decision-making were compared to identical button presses that were experimentally specified rather than selected by the subject. Magnetoencephalography (MEG) was used to record neural activity during these—what are being termed—free and forced actions and differences in the MEG signal between these two conditions were attributed to the distinct forms of neural activity required to carry out the two types of actions. To produce instances of free and forced behavior, cued button-pressing experiments were performed that use visual, aural, and memorized cues to instruct experimental subjects of the expected outcome of individual trials. Classification analysis of the trials revealed that cortical regions that allowed for the most accurate classification of free and forced actions primarily handle sensory input for the modality used to cue the trials: occipital cortex for visually cued trials, temporal cortex for aurally cued trials, and minor non-localized differences in MEG activity for trials initiated from memory. The differential roles of visual and auditory sensory cortices during free and forced actions provided insight into the neural processing steps that were engaged to initiate cued actions. Specifically, it suggested that detectable differences exist in the activity of sensory cortices and their target sites when subjects performed free and forced actions in response to sensory cues. PMID:23060760

  1. Potentiation of the cytotoxic action of melphalan and "activated" cyclophosphamide against cultured tumor cells by centrophenoxine.

    PubMed

    Sladek, N E

    1977-01-01

    Centrophenoxine, without antitumor activity itself, enhanced the cytotoxic action of melphalan and "activated" cyclophosphamide against mouse P388 lymphoma and rat W256 carcinosarcoma cells growing in static suspension culture. The concentration of alkylating agent required for 99% cell-kill was approximately halved when centrophenoxine was also present during exposure to the antitumor drug. Maximum potentiation by centrophenoxine of the cytotoxic action of melphalan occurred when cells were exposed to the two agents simultaneously; little or no potentiation was observed when cells were exposed to centrophenoxine before or after exposure to the alkylating agent.

  2. Detection and classification of raw action potential patterns in human Muscle Sympathetic Nerve Activity.

    PubMed

    Salmanpour, Aryan; Brown, Lyndon J; Shoemaker, J K

    2008-01-01

    The Muscle Sympathetic Nerve Activity (MSNA) consists of synchronous neural discharges separated by periods of neural silence dominated by heavy background noise. During measurement with electrodes, the raw MSNA signal is amplified, band-pass filtered, rectified and integrated. This integration process removes much neurophysiological information. In this paper a method for detecting a raw action potential (before the pre-amplifier) and filtered action potential (after the band-pass filter) is presented. This method is based on stationary wavelet transform (SWT) and a peak detection algorithm. Also, the detected action potentials were clustered using the k-means method and the cluster averages were calculated. The action potential detector and classification algorithm are evaluated using real MSNA recorded from three healthy subjects.

  3. 19 CFR 207.20 - Investigative activity following preliminary determination.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Investigative activity following preliminary determination. 207.20 Section 207.20 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION NONADJUDICATIVE INVESTIGATIONS INVESTIGATIONS OF WHETHER INJURY TO DOMESTIC INDUSTRIES RESULTS FROM IMPORTS SOLD AT LESS THAN FAIR VALUE OR FROM...

  4. Population of Computational Rabbit-Specific Ventricular Action Potential Models for Investigating Sources of Variability in Cellular Repolarisation

    PubMed Central

    Gemmell, Philip; Burrage, Kevin; Rodriguez, Blanca; Quinn, T. Alexander

    2014-01-01

    Variability is observed at all levels of cardiac electrophysiology. Yet, the underlying causes and importance of this variability are generally unknown, and difficult to investigate with current experimental techniques. The aim of the present study was to generate populations of computational ventricular action potential models that reproduce experimentally observed intercellular variability of repolarisation (represented by action potential duration) and to identify its potential causes. A systematic exploration of the effects of simultaneously varying the magnitude of six transmembrane current conductances (transient outward, rapid and slow delayed rectifier K+, inward rectifying K+, L-type Ca2+, and Na+/K+ pump currents) in two rabbit-specific ventricular action potential models (Shannon et al. and Mahajan et al.) at multiple cycle lengths (400, 600, 1,000 ms) was performed. This was accomplished with distributed computing software specialised for multi-dimensional parameter sweeps and grid execution. An initial population of 15,625 parameter sets was generated for both models at each cycle length. Action potential durations of these populations were compared to experimentally derived ranges for rabbit ventricular myocytes. 1,352 parameter sets for the Shannon model and 779 parameter sets for the Mahajan model yielded action potential duration within the experimental range, demonstrating that a wide array of ionic conductance values can be used to simulate a physiological rabbit ventricular action potential. Furthermore, by using clutter-based dimension reordering, a technique that allows visualisation of multi-dimensional spaces in two dimensions, the interaction of current conductances and their relative importance to the ventricular action potential at different cycle lengths were revealed. Overall, this work represents an important step towards a better understanding of the role that variability in current conductances may play in experimentally observed

  5. Being Active and Impulsive: The Role of Goals for Action and Inaction in Self-Control.

    PubMed

    Hepler, Justin; Albarracin, Dolores; McCulloch, Kathleen C; Noguchi, Kenji

    2012-12-01

    Although self-control often requires behavioral inaction (i.e., not eating a piece of cake), the process of inhibiting impulsive behavior is commonly characterized as cognitively active (i.e., actively exerting self-control). Two experiments examined whether motivation for action or inaction facilitates self-control behavior in the presence of tempting stimuli. Experiment 1 used a delay discounting task to assess the ability to delay gratification with respect to money. Experiment 2 used a Go/No-Go task to assess the ability to inhibit a dominant but incorrect motor response to the words "condom" and "sex". The results demonstrate that goals for inaction promote self-control, whereas goals for action promote impulsive behavior. These findings are discussed in light of recent evidence suggesting that goals for action and inaction modulate physiological resources that promote behavioral execution. PMID:23766548

  6. Observing Children's Learning: Informing Effective Intervention. A Personal Story of Investigative Research in Action.

    ERIC Educational Resources Information Center

    Lockett, Andrew

    This paper outlines the underlying principles that have guided the development of an observational orientation to assessing children's learning. The development of an observation orientation was achieved through a process of a number of action-type research projects within a range of early years settings in the United Kingdom. The paper outlines a…

  7. Using Action Research to Investigate the Use of Digital Information Resources in Further Education

    ERIC Educational Resources Information Center

    Rowley, Jennifer; Ray, Kathryn; Proud, Debbie; Banwell, Linda; Spink, Sian; Thomas, Rhian; Urquhart, Christine

    2004-01-01

    Within the Third Annual Cycle of the Joint Information Systems Committee (JISC) User Behaviour Monitoring and Evaluation Framework, six small-scale action research interventions were undertaken in further education (FE) institutions. The aims of these interventions were two-fold: (i) to develop understanding of the facilitators and barriers to the…

  8. Learning to associate novel words with motor actions: language-induced motor activity following short training.

    PubMed

    Fargier, Raphaël; Paulignan, Yves; Boulenger, Véronique; Monaghan, Padraic; Reboul, Anne; Nazir, Tatjana A

    2012-07-01

    Action words referring to face, arm or leg actions activate areas along the motor strip that also control the planning and execution of the actions specified by the words. This electroencephalogram (EEG) study aimed to test the learning profile of this language-induced motor activity. Participants were trained to associate novel verbal stimuli to videos of object-oriented hand and arm movements or animated visual images on two consecutive days. Each training session was preceded and followed by a test-session with isolated videos and verbal stimuli. We measured motor-related brain activity (reflected by a desynchronization in the μ frequency bands; 8-12 Hz range) localized at centro-parietal and fronto-central electrodes. We compared activity from viewing the videos to activity resulting from processing the language stimuli only. At centro-parietal electrodes, stable action-related μ suppression was observed during viewing of videos in each test-session of the two days. For processing of verbal stimuli associated with motor actions, a similar pattern of activity was evident only in the second test-session of Day 1. Over the fronto-central regions, μ suppression was observed in the second test-session of Day 2 for the videos and in the second test-session of Day 1 for the verbal stimuli. Whereas the centro-parietal μ suppression can be attributed to motor events actually experienced during training, the fronto-central μ suppression seems to serve as a convergence zone that mediates underspecified motor information. Consequently, sensory-motor reactivations through which concepts are comprehended seem to differ in neural dynamics from those implicated in their acquisition.

  9. Pre & Postsynaptic Tuning of Action Potential Timing by Spontaneous GABAergic Activity

    PubMed Central

    Caillard, Olivier

    2011-01-01

    Frequency and timing of action potential discharge are key elements for coding and transfer of information between neurons. The nature and location of the synaptic contacts, the biophysical parameters of the receptor-operated channels and their kinetics of activation are major determinants of the firing behaviour of each individual neuron. Ultimately the intrinsic excitability of each neuron determines the input-output function. Here we evaluate the influence of spontaneous GABAergic synaptic activity on the timing of action potentials in Layer 2/3 pyramidal neurones in acute brain slices from the somatosensory cortex of young rats. Somatic dynamic current injection to mimic synaptic input events was employed, together with a simple computational model that reproduce subthreshold membrane properties. Besides the well-documented control of neuronal excitability, spontaneous background GABAergic activity has a major detrimental effect on spike timing. In fact, GABAA receptors tune the relationship between the excitability and fidelity of pyramidal neurons via a postsynaptic (the reversal potential for GABAA activity) and a presynaptic (the frequency of spontaneous activity) mechanism. GABAergic activity can decrease or increase the excitability of pyramidal neurones, depending on the difference between the reversal potential for GABAA receptors and the threshold for action potential. In contrast, spike time jitter can only be increased proportionally to the difference between these two membrane potentials. Changes in excitability by background GABAergic activity can therefore only be associated with deterioration of the reliability of spike timing. PMID:21789249

  10. An Action Research Study on the Effect of Interactive Technology and Active Learning on Student Performance

    ERIC Educational Resources Information Center

    Bear, Teresa J.

    2013-01-01

    This quantitative action science research study utilized a causal-comparative experimental research design in order to determine if the use of student response systems (clickers), as an active learning strategy in a community college course, improved student performance in the course. Students in the experimental group (n = 26) used clickers to…

  11. 76 FR 3150 - Office of Biotechnology Activities; Recombinant DNA Research: Action Under the NIH Guidelines for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-19

    ...). On July 20, 2010 the NIH Office of Biotechnology Activities (OBA) published a proposed action (75 FR... exception of rodents that contain a transgene encoding more than fifty percent of an exogenous eukaryotic... percent of the genome of an exogenous eukaryotic virus from a single family, in order to...

  12. 78 FR 17702 - Agency Information Collection Activities: Application for Action on an Approved Application or...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-22

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Application... Collection ACTION: 60-Day Notice. SUMMARY: The Department of Homeland Security (DHS), U.S. Citizenship and... Coordination Division, Office of Policy and Strategy, U.S. Citizenship and Immigration Services, Department...

  13. Activity-Based Teaching in Social Studies Education: An Action Research

    ERIC Educational Resources Information Center

    Akkus, Zekerya

    2015-01-01

    The aim of this study was to determine pre-service social studies teachers' skills to plan and apply the activity-based teaching and contribute to their development of these skills. In the study, the action research design of qualitative research was used. The sample of the study consisted of 6 pre-service teachers who were 4th year students at…

  14. 75 FR 69687 - Office of Biotechnology Activities Recombinant DNA Research: Proposed Actions Under the NIH...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-15

    ... HUMAN SERVICES National Institutes of Health Office of Biotechnology Activities Recombinant DNA Research: Proposed Actions Under the NIH Guidelines for Research Involving Recombinant DNA Molecules (NIH Guidelines... the NIH Recombinant DNA Advisory Committee (RAC) and specifically approved by the NIH Director as...

  15. 78 FR 12074 - Office of Biotechnology Activities; Recombinant DNA Research: Actions Under the NIH Guidelines...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-21

    ... containing an HA from the Goose/Guangdong/1/96 lineage should become an HHS Select Agent (77 FR 63783... HUMAN SERVICES National Institutes of Health Office of Biotechnology Activities; Recombinant DNA Research: Actions Under the NIH Guidelines for Research Involving Recombinant DNA Molecules (NIH...

  16. 75 FR 28811 - Office of Biotechnology Activities; Recombinant DNA Research: Proposed Actions Under the NIH...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-24

    ... HUMAN SERVICES National Institutes of Health Office of Biotechnology Activities; Recombinant DNA Research: Proposed Actions Under the NIH Guidelines for Research Involving Recombinant DNA Molecules (NIH... DNA Advisory Committee and approved by the NIH Director (Section III-A-1). Such research involves...

  17. 76 FR 62816 - Office of Biotechnology Activities; Recombinant DNA Research: Action Under the NIH Guidelines for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... experts from NIH, CDC, and academia. These proposed changes were published in the Federal Register (76 FR... HUMAN SERVICES National Institutes of Health Office of Biotechnology Activities; Recombinant DNA Research: Action Under the NIH Guidelines for Research Involving Recombinant DNA Molecules (NIH...

  18. Science and Social Practice: Action Research and Activity Theory as Socio-Critical Approaches

    ERIC Educational Resources Information Center

    Langemeyer, Ines

    2011-01-01

    Action research and activity theory are considered by a number of followers as socio-critical approaches, whereas others do not relate them to social-criticism and use them merely as methods to improve practice. This article searches for general insights in Kurt Lewin's and Lev S. Vygotsky's work into how one proceeds and acts critically. In their…

  19. Empowering Parents: Developing Support, Leadership, Advocacy, and Activism. Child Care Action Campaign Issue Brief #2.

    ERIC Educational Resources Information Center

    Miller, Laurie; Anderson, Candice

    This report from a 1995 Child Care Action Campaign national audioconference examines approaches to empowering parents through developing support, leadership, advocacy, and activism to better enable low-income parents to become effective change agents. The report describes the experiences of three parent programs, which found that parents typically…

  20. 77 FR 3460 - Reimbursement for Costs of Remedial Action at Active Uranium and Thorium Processing Sites

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-24

    ... published a final rule under 10 CFR Part 765 in the Federal Register on May 23, 1994, (59 FR 26714) to carry... for reimbursement. DOE amended the final rule on June 3, 2003, (68 FR 32955) to adopt several... Reimbursement for Costs of Remedial Action at Active Uranium and Thorium Processing Sites AGENCY: Department...

  1. 75 FR 71677 - Reimbursement for Costs of Remedial Action at Active Uranium and Thorium Processing Sites

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-24

    ... published a final rule under 10 CFR Part 765 in the Federal Register on May 23, 1994, (59 FR 26714) to carry... for reimbursement. DOE amended the final rule on June 3, 2003, (68 FR 32955) to adopt several... Reimbursement for Costs of Remedial Action at Active Uranium and Thorium Processing Sites AGENCY: Department...

  2. Corrective Action Investigation Plan for Corrective Action Unit 5: Landfills, Nevada Test Site, Nevada (Rev. No.: 0) includes Record of Technical Change No. 1 (dated 9/17/2002)

    SciTech Connect

    IT Corporation, Las Vegas, NV

    2002-05-28

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 5 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 5 consists of eight Corrective Action Sites (CASs): 05-15-01, Sanitary Landfill; 05-16-01, Landfill; 06-08-01, Landfill; 06-15-02, Sanitary Landfill; 06-15-03, Sanitary Landfill; 12-15-01, Sanitary Landfill; 20-15-01, Landfill; 23-15-03, Disposal Site. Located between Areas 5, 6, 12, 20, and 23 of the Nevada Test Site (NTS), CAU 5 consists of unlined landfills used in support of disposal operations between 1952 and 1992. Large volumes of solid waste were produced from the projects which used the CAU 5 landfills. Waste disposed in these landfills may be present without appropriate controls (i.e., use restrictions, adequate cover) and hazardous and/or radioactive constituents may be present at concentrations and locations that could potentially pose a threat to human health and/or the environment. During the 1992 to 1995 time frame, the NTS was used for various research and development projects including nuclear weapons testing. Instead of managing solid waste at one or two disposal sites, the practice on the NTS was to dispose of solid waste in the vicinity of the project. A review of historical documentation, process knowledge, personal interviews, and inferred activities associated with this CAU identified the following as potential contaminants of concern: volatile organic compounds, semivolatile organic compounds, polychlorinated biphenyls, pesticides, petroleum hydrocarbons (diesel- and gasoline-range organics), Resource Conservation and Recovery Act Metals, plus nickel and zinc. A two-phase approach has been selected to collect information and generate data to satisfy needed resolution criteria

  3. Adolescents' Physical Activity at Recess and Actions to Promote a Physically Active School Day in Four Finnish Schools

    ERIC Educational Resources Information Center

    Haapala, H. L.; Hirvensalo, M. H.; Laine, K.; Laakso, L.; Hakonen, H.; Lintunen, T.; Tammelin, T. H.

    2014-01-01

    The national Finnish Schools on the Move programme support schools with their individual plans to promote school-based physical activity (PA). We examined the changes in adolescents' recess and overall PA in four lower secondary schools and described the school actions to promote students' PA and the local contact persons'…

  4. Selecting object pairs for action: Is the active object always first?

    PubMed

    Laverick, Rosanna; Wulff, Melanie; Honisch, Juliane J; Chua, Wei Ling; Wing, Alan M; Rotshtein, Pia

    2015-08-01

    Perception is linked to action via two routes: a direct route based on affordance information in the environment and an indirect route based on semantic knowledge about objects. The present study explored the factors modulating the recruitment of the two routes, in particular which factors affecting the selection of paired objects. In Experiment 1, we presented real objects among semantically related or unrelated distracters. Participants had to select two objects that can interact. The presence of distracters affected selection times, but not the semantic relations of the objects with the distracters. Furthermore, participants first selected the active object (e.g. teaspoon) with their right hand, followed by the passive object (e.g. mug), often with their left hand. In Experiment 2, we presented pictures of the same objects with no hand grip, congruent or incongruent hand grip. Participants had to decide whether the two objects can interact. Action decisions were faster when the presentation of the active object preceded the presentation of the passive object, and when the grip was congruent. Interestingly, participants were slower when the objects were semantically but not functionally related; this effect increased with congruently gripped objects. Our data showed that action decisions in the presence of strong affordance cues (real objects, pictures of congruently gripped objects) relied on sensory-motor representation, supporting the direct route from perception-to-action that bypasses semantic knowledge. However, in the case of weak affordance cues (pictures), semantic information interfered with action decisions, indicating that semantic knowledge impacts action decisions. The data support the dual-route account from perception-to-action.

  5. Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans

    PubMed Central

    Li, Wen-Ru; Shi, Qing-Shan; Dai, Huan-Qin; Liang, Qing; Xie, Xiao-Bao; Huang, Xiao-Mo; Zhao, Guang-Ze; Zhang, Li-Xin

    2016-01-01

    The antifungal activity, kinetics, and molecular mechanism of action of garlic oil against Candida albicans were investigated in this study using multiple methods. Using the poisoned food technique, we determined that the minimum inhibitory concentration of garlic oil was 0.35 μg/mL. Observation by transmission electron microscopy indicated that garlic oil could penetrate the cellular membrane of C. albicans as well as the membranes of organelles such as the mitochondria, resulting in organelle destruction and ultimately cell death. RNA sequencing analysis showed that garlic oil induced differential expression of critical genes including those involved in oxidation-reduction processes, pathogenesis, and cellular response to drugs and starvation. Moreover, the differentially expressed genes were mainly clustered in 19 KEGG pathways, representing vital cellular processes such as oxidative phosphorylation, the spliceosome, the cell cycle, and protein processing in the endoplasmic reticulum. In addition, four upregulated proteins selected after two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) analysis were identified with high probability by mass spectrometry as putative cytoplasmic adenylate kinase, pyruvate decarboxylase, hexokinase, and heat shock proteins. This is suggestive of a C. albicans stress responses to garlic oil treatment. On the other hand, a large number of proteins were downregulated, leading to significant disruption of the normal metabolism and physical functions of C. albicans. PMID:26948845

  6. Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans.

    PubMed

    Li, Wen-Ru; Shi, Qing-Shan; Dai, Huan-Qin; Liang, Qing; Xie, Xiao-Bao; Huang, Xiao-Mo; Zhao, Guang-Ze; Zhang, Li-Xin

    2016-01-01

    The antifungal activity, kinetics, and molecular mechanism of action of garlic oil against Candida albicans were investigated in this study using multiple methods. Using the poisoned food technique, we determined that the minimum inhibitory concentration of garlic oil was 0.35 μg/mL. Observation by transmission electron microscopy indicated that garlic oil could penetrate the cellular membrane of C. albicans as well as the membranes of organelles such as the mitochondria, resulting in organelle destruction and ultimately cell death. RNA sequencing analysis showed that garlic oil induced differential expression of critical genes including those involved in oxidation-reduction processes, pathogenesis, and cellular response to drugs and starvation. Moreover, the differentially expressed genes were mainly clustered in 19 KEGG pathways, representing vital cellular processes such as oxidative phosphorylation, the spliceosome, the cell cycle, and protein processing in the endoplasmic reticulum. In addition, four upregulated proteins selected after two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) analysis were identified with high probability by mass spectrometry as putative cytoplasmic adenylate kinase, pyruvate decarboxylase, hexokinase, and heat shock proteins. This is suggestive of a C. albicans stress responses to garlic oil treatment. On the other hand, a large number of proteins were downregulated, leading to significant disruption of the normal metabolism and physical functions of C. albicans. PMID:26948845

  7. Microbial and immunological investigations and remedial action after an outbreak of humidifier fever

    PubMed Central

    Edwards, J H

    1980-01-01

    ABSTRACT Humidifier fever (Monday sickness) occuring in office staff in a factory processing rayon presented as pyrexia with a polyuria and leucocytosis on the first day back to work after a break during the winter half of the year. Chest radiographs showed no abnormalities but pulmonary function tests indicated mild airways obstruction in the affected group as a whole. Respirable dust samples taken on a Monday when 11 cases occured were not pyrogenic, indicating that a mechanism other than direct pyrogen activity produced the pyrexia. Efforts were then directed to determining an immunological basis for the episodes. In particular, Thermoactinomyces vulgaris, previously held responsible for humidifier fever, was studied. During the episode of 11 cases, the number of viable airborne spores of this organism was far higher than on Mondays when no cases occured. In a second episode of nine cases, however, the airborne viable count was of the same order as non-episode Mondays. Extracts of T vulgaris produced lines of precipitation in gel diffusion studies with roughly half the office staff sera tested, but no correlation was observed between precipitin line formation and disease. A similar proportion of normal sera reacted against this extract. Extracts of dust lying on the topside surface of the suspended ceiling above the office, however, produced precipitin lines with sera from 16/18 affected individuals and 2/18 non-affected individuals (p < 0·001) as did extracts of humidifier material. Extensive microbial analysis failed to detect any one fungus or bacterium that produced antigens capable of reacting with positive serum, but extracts of amoebae correlated absolutely with humidifier material and ceiling dust extract in gel diffusion studies. A reaction of identity observed between the amoebae and ceiling dust extracts showed the presence of identical antigens. In similar studies the high degree of cross reactivity with antigens and sera from Spanish and Swedish

  8. Action Memorandum for General Decommissioning Activities under the Idaho Cleanup Project

    SciTech Connect

    S. L. Reno

    2006-10-26

    This Action Memorandum documents the selected alternative to perform general decommissioning activities at the Idaho National Laboratory (INL) under the Idaho Cleanup Project (ICP). Preparation of this Action Memorandum has been performed in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), as amended by the "Superfund Amendments and Reauthorization Act of 1986", and in accordance with the "National Oil and Hazardous Substances Pollution Contingency Plan". An engineering evaluation/cost analysis (EE/CA) was prepared and released for public comment and evaluated alternatives to accomplish the decommissioning of excess buildings and structures whose missions havve been completed.

  9. Action semantic knowledge about objects is supported by functional motor activation.

    PubMed

    van Elk, Michiel; van Schie, Hein T; Bekkering, Harold

    2009-08-01

    The present study assessed the functional organization of action semantics by asking subjects to categorize pictures of an actor holding objects with a correct or incorrect grip at either a correct or incorrect goal location. Overall, reaction times were slower if the object was presented with an inappropriate posture, and this effect was stronger for goal violations compared with grip violations (Experiment 1). In addition, the retrieval of action semantics was found accompanied by the implicit activation of motor representations. Body-related objects (e.g., cup) were classified faster when a movement toward the subject's body was required, whereas world-related objects (e.g., pincers) were responded to faster with a movement in the opposite direction (Experiments 2 and 3). In contrast, when subjects were required to retrieve only visual semantics (Experiment 4), no interference effects of postural information were observed, and motor representations were only partially activated. These findings suggest that action semantics can be accessed independently from visual semantics and that the retrieval of action semantics is supported by functional motor activation reflecting the prototypical use of an object.

  10. Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential.

    PubMed

    Desbois, Andrew P; Smith, Valerie J

    2010-02-01

    Amongst the diverse and potent biological activities of free fatty acids (FFAs) is the ability to kill or inhibit the growth of bacteria. The antibacterial properties of FFAs are used by many organisms to defend against parasitic or pathogenic bacteria. Whilst their antibacterial mode of action is still poorly understood, the prime target of FFA action is the cell membrane, where FFAs disrupt the electron transport chain and oxidative phosphorylation. Besides interfering with cellular energy production, FFA action may also result from the inhibition of enzyme activity, impairment of nutrient uptake, generation of peroxidation and auto-oxidation degradation products or direct lysis of bacterial cells. Their broad spectrum of activity, non-specific mode of action and safety makes them attractive as antibacterial agents for various applications in medicine, agriculture and food preservation, especially where the use of conventional antibiotics is undesirable or prohibited. Moreover, the evolution of inducible FFA-resistant phenotypes is less problematic than with conventional antibiotics. The potential for commercial or biomedical exploitation of antibacterial FFAs, especially for those from natural sources, is discussed.

  11. Quinoxaline 1,4-di-N-Oxides: Biological Activities and Mechanisms of Actions.

    PubMed

    Cheng, Guyue; Sa, Wei; Cao, Chen; Guo, Liangliang; Hao, Haihong; Liu, Zhenli; Wang, Xu; Yuan, Zonghui

    2016-01-01

    Quinoxaline 1,4-di-N-oxides (QdNOs) have manifold biological properties, including antimicrobial, antitumoral, antitrypanosomal and antiinflammatory/antioxidant activities. These diverse activities endow them broad applications and prospects in human and veterinary medicines. As QdNOs arouse widespread interest, the evaluation of their medicinal chemistry is still in progress. In the meantime, adverse effects have been reported in some of the QdNO derivatives. For example, genotoxicity and bacterial resistance have been found in QdNO antibacterial growth promoters, conferring urgent need for discovery of new QdNO drugs. However, the modes of actions of QdNOs are not fully understood, hindering the development and innovation of these promising compounds. Here, QdNOs are categorized based on the activities and usages, among which the antimicrobial activities are consist of antibacterial, antimycobacterial and anticandida activities, and the antiprotozoal activities include antitrypanosomal, antimalarial, antitrichomonas, and antiamoebic activities. The structure-activity relationship and the mode of actions of each type of activity of QdNOs are summarized, and the toxicity and the underlying mechanisms are also discussed, providing insight for the future research and development of these fascinating compounds.

  12. Pivotal role of tissue plasminogen activator in the mechanism of action of electroconvulsive therapy.

    PubMed

    Hoirisch-Clapauch, Silvia; Mezzasalma, Marco A U; Nardi, Antonio E

    2014-02-01

    Electroconvulsive therapy is an important treatment option for major depressive disorders, acute mania, mood disorders with psychotic features, and catatonia. Several hypotheses have been proposed as electroconvulsive therapy's mechanism of action. Our hypothesis involves many converging pathways facilitated by increased synthesis and release of tissue-plasminogen activator. Human and animal experiments have shown that tissue-plasminogen activator participates in many mechanisms of action of electroconvulsive therapy or its animal variant, electroconvulsive stimulus, including improved N-methyl-D-aspartate receptor-mediated signaling, activation of both brain-derived neurotrophic factor and vascular endothelial growth factor, increased bioavailability of zinc, purinergic release, and increased mobility of dendritic spines. As a result, tissue-plasminogen activator helps promote neurogenesis in limbic structures, modulates synaptic transmission and plasticity, improves cognitive function, and mediates antidepressant effects. Notably, electroconvulsive therapy seems to influence tissue-plasminogen activator metabolism. For example, electroconvulsive stimulus increases the expression of glutamate decarboxylase 65 isoform in γ-aminobutyric acid-releasing neurons, which enhances the release of tissue-plasminogen activator, and the expression of p11, a protein involved in plasminogen and tissue-plasminogen activator assembling. This paper reviews how electroconvulsive therapy correlates with tissue-plasminogen activator. We suggest that interventions aiming at increasing tissue-plasminogen activator levels or its bioavailability - such as daily aerobic exercises together with a carbohydrate-restricted diet, or normalization of homocysteine levels - be evaluated in controlled studies assessing response and remission duration in patients who undergo electroconvulsive therapy.

  13. Quinoxaline 1,4-di-N-Oxides: Biological Activities and Mechanisms of Actions

    PubMed Central

    Cheng, Guyue; Sa, Wei; Cao, Chen; Guo, Liangliang; Hao, Haihong; Liu, Zhenli; Wang, Xu; Yuan, Zonghui

    2016-01-01

    Quinoxaline 1,4-di-N-oxides (QdNOs) have manifold biological properties, including antimicrobial, antitumoral, antitrypanosomal and antiinflammatory/antioxidant activities. These diverse activities endow them broad applications and prospects in human and veterinary medicines. As QdNOs arouse widespread interest, the evaluation of their medicinal chemistry is still in progress. In the meantime, adverse effects have been reported in some of the QdNO derivatives. For example, genotoxicity and bacterial resistance have been found in QdNO antibacterial growth promoters, conferring urgent need for discovery of new QdNO drugs. However, the modes of actions of QdNOs are not fully understood, hindering the development and innovation of these promising compounds. Here, QdNOs are categorized based on the activities and usages, among which the antimicrobial activities are consist of antibacterial, antimycobacterial and anticandida activities, and the antiprotozoal activities include antitrypanosomal, antimalarial, antitrichomonas, and antiamoebic activities. The structure-activity relationship and the mode of actions of each type of activity of QdNOs are summarized, and the toxicity and the underlying mechanisms are also discussed, providing insight for the future research and development of these fascinating compounds. PMID:27047380

  14. Baking together-the coordination of actions in activities involving people with dementia.

    PubMed

    Majlesi, Ali Reza; Ekström, Anna

    2016-08-01

    This study explores interaction and collaboration between people with dementia and their spouses in relation to the performance of household chores with the focus on instruction as an interactional context to engage the person with dementia in collaboration to accomplish joint activities. Dementia is generally associated with pathological changes in people's cognitive functions such as diminishing memory functions, communicative abilities and also diminishing abilities to take initiative as well as to plan and execute tasks. Using video recordings of everyday naturally occurring activities, we analyze the sequential organization of actions (see Schegloff, 2007) oriented toward the accomplishment of a joint multi-task activity of baking. The analysis shows the specific ways of collaboration through instructional activities in which the person with dementia exhibits his competence and skills in accomplishing the given tasks through negotiating the instructions with his partner and carrying out instructed actions. Although the driving force of the collaboration seems to be a series of directive sequences only initiated by the partner throughout the baking activity, our analyses highlight how the person with dementia can actively use the material environment-including collaborating partners-to compensate for challenges and difficulties encountered in achieving everyday tasks. The sequential organization of instructions and instructed actions are in this sense argued to provide an interactional environment wherein the person with dementia can make contributions to the joint activity in an efficient way. While a collaborator has been described as necessary for a person with dementia to be able to partake in activities, this study shows that people with dementia are not only guided by their collaborators in joint activities but they can also actively use their collaborators in intricate compensatory ways.

  15. Baking together-the coordination of actions in activities involving people with dementia.

    PubMed

    Majlesi, Ali Reza; Ekström, Anna

    2016-08-01

    This study explores interaction and collaboration between people with dementia and their spouses in relation to the performance of household chores with the focus on instruction as an interactional context to engage the person with dementia in collaboration to accomplish joint activities. Dementia is generally associated with pathological changes in people's cognitive functions such as diminishing memory functions, communicative abilities and also diminishing abilities to take initiative as well as to plan and execute tasks. Using video recordings of everyday naturally occurring activities, we analyze the sequential organization of actions (see Schegloff, 2007) oriented toward the accomplishment of a joint multi-task activity of baking. The analysis shows the specific ways of collaboration through instructional activities in which the person with dementia exhibits his competence and skills in accomplishing the given tasks through negotiating the instructions with his partner and carrying out instructed actions. Although the driving force of the collaboration seems to be a series of directive sequences only initiated by the partner throughout the baking activity, our analyses highlight how the person with dementia can actively use the material environment-including collaborating partners-to compensate for challenges and difficulties encountered in achieving everyday tasks. The sequential organization of instructions and instructed actions are in this sense argued to provide an interactional environment wherein the person with dementia can make contributions to the joint activity in an efficient way. While a collaborator has been described as necessary for a person with dementia to be able to partake in activities, this study shows that people with dementia are not only guided by their collaborators in joint activities but they can also actively use their collaborators in intricate compensatory ways. PMID:27531451

  16. Between control and hacker activism: the political actions of Anonymous Brasil.

    PubMed

    Machado, Murilo Bansi

    2015-12-01

    This paper addresses the political actions of Anonymous, the principal expression of current hacker activism, arguing that hacktivism is a form of political resistance in control societies. To this end, it focuses on studying the Brazilian, hacktivist facet of the collective. In order to stress its political character, it scrutinizes the principal expressions of hacking in the literature. It describes motivations, methods and the ethics of its political actions, based on a comparative analysis of two operations carried out by Brazilian Anonymous adherents in 2012: #OpWeeksPayment and #OpGlobo. And it finishes by identifying four of its main forms of political engagement: promotion of anonymity; "evangelization;" the formation of distributed networks; and the fact that the collective carries out and facilitates several types of political actions. PMID:26785867

  17. Between control and hacker activism: the political actions of Anonymous Brasil.

    PubMed

    Machado, Murilo Bansi

    2015-12-01

    This paper addresses the political actions of Anonymous, the principal expression of current hacker activism, arguing that hacktivism is a form of political resistance in control societies. To this end, it focuses on studying the Brazilian, hacktivist facet of the collective. In order to stress its political character, it scrutinizes the principal expressions of hacking in the literature. It describes motivations, methods and the ethics of its political actions, based on a comparative analysis of two operations carried out by Brazilian Anonymous adherents in 2012: #OpWeeksPayment and #OpGlobo. And it finishes by identifying four of its main forms of political engagement: promotion of anonymity; "evangelization;" the formation of distributed networks; and the fact that the collective carries out and facilitates several types of political actions.

  18. In Vivo Antimalarial Activity and Mechanisms of Action of 4-Nerolidylcatechol Derivatives

    PubMed Central

    Rocha e Silva, Luiz Francisco; Nogueira, Karla Lagos; Pinto, Ana Cristina da Silva; Katzin, Alejandro Miguel; Sussmann, Rodrigo A. C.; Muniz, Magno Perêa; Neto, Valter Ferreira de Andrade; Chaves, Francisco Célio Maia; Coutinho, Julia Penna; Lima, Emerson Silva; Krettli, Antoniana Ursine; Tadei, Wanderli Pedro

    2015-01-01

    4-Nerolidylcatechol (1) is an abundant antiplasmodial metabolite that is isolated from Piper peltatum roots. O-Acylation or O-alkylation of compound 1 provides derivatives exhibiting improved stability and significant in vitro antiplasmodial activity. The aim of this work was to study the in vitro inhibition of hemozoin formation, inhibition of isoprenoid biosynthesis in Plasmodium falciparum cultures, and in vivo antimalarial activity of several 4-nerolidylcatechol derivatives. 1,2-O,O-Diacetyl-4-nerolidylcatechol (2) inhibited in vitro hemozoin formation by up to 50%. In metabolic labeling studies using [1-(n)-3H]geranylgeranyl pyrophosphate, diester 2 significantly inhibited the biosynthesis of isoprenoid metabolites ubiquinone 8, menaquinone 4, and dolichol 12 in cultures of P. falciparum 3D7. Similarly, 2-O-benzyl-4-nerolidylcatechol (3) significantly inhibited the biosynthesis of dolichol 12. P. falciparum in vitro protein synthesis was not affected by compounds 2 or 3. At oral doses of 50 mg per kg of body weight per day, compound 2 suppressed Plasmodium berghei NK65 in infected BALB/c mice by 44%. This in vivo result for derivative 2 represents marked improvement over that obtained previously for natural product 1. Compound 2 was not detected in mouse blood 1 h after oral ingestion or in mixtures with mouse blood/blood plasma in vitro. However, it was detected after in vitro contact with human blood or blood plasma. Derivatives of 4-nerolidylcatechol exhibit parasite-specific modes of action, such as inhibition of isoprenoid biosynthesis and inhibition of hemozoin formation, and they therefore merit further investigation for their antimalarial potential. PMID:25801563

  19. Investigation of molecular mechanisms of action of chelating drugs on protein-lipid model membranes by X-ray fluorescence

    SciTech Connect

    Novikova, N. N.; Zheludeva, S. I.; Koval'chuk, M. V.; Stepina, N. D.; Erko, A. I.; Yur'eva, E. A.

    2009-12-15

    Protein-lipid films based on the enzyme alkaline phosphatase were subjected to the action of chelating drugs, which are used for accelerating the removal of heavy metals from the human body, and the elemental composition of the resulting films was investigated. Total-reflection X-ray fluorescence measurements were performed at the Berlin Electron Storage Ring Company for Synchrotron Radiation (BESSY) in Germany. A comparative estimation of the protective effect of four drugs (EDTA, succimer, xydiphone, and mediphon) on membrane-bound enzymes damaged by lead ions was made. The changes in the elemental composition of the protein-lipid films caused by high doses of chelating drugs were investigated. It was shown that state-of-the-art X-ray techniques can, in principle, be used to develop new methods for the in vitro evaluation of the efficiency of drugs, providing differential data on their actions.

  20. Optical clearing of skin under action of glycerol: Ex vivo and in vivo investigations

    NASA Astrophysics Data System (ADS)

    Genina, E. A.; Bashkatov, A. N.; Sinichkin, Yu. P.; Tuchin, V. V.

    2010-08-01

    The behavior of optical parameters of the skin of a laboratory rat under the action of an aqueous solution of glycerol is studied ex vivo and in vivo. It is found that the collimated transmission coefficient of ex vivo skin samples increases by a factor of 20-40-fold depending on the wavelength in the studied spectral range, and the diffuse reflection coefficient of skin in vivo decreases on the average by 16%. The results presented can be useful for many methods of laser therapy and optical diagnostics of skin diseases and localization of subcutaneous neoplasms.

  1. Evaluation of the legal consequences of action affects neural activity and emotional experience during the resolution of moral dilemmas.

    PubMed

    Pletti, Carolina; Sarlo, Michela; Palomba, Daniela; Rumiati, Rino; Lotto, Lorella

    2015-03-01

    In any modern society killing is regarded as a severe violation of the legal codes that is subjected to penal judgment. Therefore, it is likely that people take legal consequences into account when deciding about the hypothetical killing of one person in classic moral dilemmas, with legal concerns contributing to decision-making. In particular, by differing for the degree of intentionality and emotional salience, Footbridge- and Trolley-type dilemmas might promote differential assignment of blame and punishment while implicating the same severity of harm. The present study was aimed at comparing the neural activity, subjective emotional reactions, and behavioral choices in two groups of participants who either took (Legal group) or did not take (No Legal group) legal consequences into account when deciding on Footbridge-type and Trolley-type moral dilemmas. Stimulus- and response-locked ERPs were measured to investigate the neural activity underlying two separate phases of the decision process. No difference in behavioral choices was found between groups. However, the No Legal group reported greater overall emotional impact, associated with lower preparation for action, suggesting greater conflict between alternative motor responses representing the different decision choices. In contrast, the Legal group showed an overall dampened affective experience during decision-making associated with greater overall action readiness and intention to act, reflecting lower conflict in responding. On these bases, we suggest that in moral dilemmas legal consequences of actions provide a sort of reference point on which people can rely to support a decision, independent of dilemma type.

  2. Emotional reactions to success and failure of collective action as predictors of future action intentions: a longitudinal investigation in the context of student protests in Germany.

    PubMed

    Tausch, Nicole; Becker, Julia C

    2013-09-01

    This research examined how emotional responses to success and failure of collective action relate to willingness to engage in collective action in the future. It was hypothesized that both pride (in relation to a success) and anger (in response to failure) would motivate future collective action. Findings are reported from a two-wave longitudinal study (N= 98) in the context of student protests against tuition fees in Germany, which was conducted before and after collective action had resulted in both a success and a failure. While anger positively predicted action intentions, over and above baseline action intentions, pride exerted a significant indirect effect on action intentions via increased efficacy perceptions, over and above baseline efficacy and action intentions. Politicized identification positively predicted the intensity of both pride and anger and baseline group efficacy positively predicted the intensity of anger. The theoretical and practical implications of these findings are discussed. PMID:22694314

  3. An investigation on the inhibitory action of benzazole derivatives as a consequence of sulfur atom induction

    NASA Astrophysics Data System (ADS)

    Moradi, Z.; Attar, M. M.

    2014-10-01

    The inhibitory action of three benzazole based molecules namely 2-methyl benzimidazole (2-MBI), 2-methyl benzothiazole (2-MBT) and 2-mercapto benzthiazole (2-SHBT) in 1 M HCl solution was studied by gravimetric analysis and electrochemical impedance spectroscopy (EIS). Results showed that the inhibitor adsorption on the iron surface was according to Langmuir adsorption isotherm for 2-MBI and 2-MBT and Flory Huggins Isotherm for 2-SHBT. Surface roughness obtained by Atomic Forced Microscopy (AFM) revealed that a good inhibitor decreases the surface roughness significantly which can be related to the formation of more integrated molecular film of inhibitor on steel surface. Based on contact angle (CA) measurements as the efficiency of the inhibitor molecules improve the hydrophobicity increases. These three molecules were chosen to see the effect of introducing sulfur atom into the structure the main effect of which would be on electronic parameters. To better understand this effect, the quantum chemical descriptors including: EHOMO, ELUMO, energy gap (ΔE), dipole moment (μ), hardness (η), softness (σ), electronegativity index (χ), fraction of electrons transferred (ΔN), that are most relevant to the potential action of a molecule as corrosion inhibitor, have been calculated in water and vacuum. Electronic parameters of these three inhibitors have been studied using DFT/B3LYP, and HF methods with 6-31G (d,p) basis set.

  4. Investigating the Antimalarial Action of 1,2,4-Trioxolanes with Fluorescent Chemical Probes

    PubMed Central

    Hartwig, Carmony L.; Lauterwasser, Erica M.W.; Mahajan, Sumit S.; Hoke, Jonathan M.; Cooper, Roland A.; Renslo, Adam R.

    2011-01-01

    The 1,2,4-trioxolanes are a new class of synthetic peroxidic antimalarials currently in human clinical trials. The well known reactivity of the 1,2,4-trioxolane ring towards inorganic ferrous iron and ferrous iron heme is proposed to play a role in the antimalarial action of this class of compounds. We have designed structurally relevant fluorescent chemical probes to study the sub-cellular localization of 1,2,4-trioxolanes in cultured Plasmodium falciparum parasites. Microscopy experiments revealed that a probe fluorescently labeled on the adamantane ring accumulated specifically in digestive vacuole-associated neutral lipid bodies within the parasite while an isosteric, but non-peroxidic congener did not. Probes fluorescently labeled on the cyclohexane ring showed no distinct localization pattern. In their sub-cellular localization and peroxidative effects, 1,2,4-trioxolane probes behave much like artemisinin-based probes studied previously. Our results are consistent with a role for adamantane-derived carbon-centered radicals in the antimalarial action of 1,2,4-trioxolanes, as hypothesized previously on the basis of chemical reactivity studies. PMID:22023506

  5. I know what I will see: action-specific motor preparation activity in a passive observation task.

    PubMed

    Bozzacchi, Chiara; Spinelli, Donatella; Pitzalis, Sabrina; Giusti, Maria Assunta; Di Russo, Francesco

    2015-06-01

    Literature on mirror neurons has shown that seeing someone preparing to move generates in the motor areas of the observers a brain activity similar to that generated when the subject prepares his own actions. Thus, the 'mirroring' of action would not be limited to the execution phase but also involves the preparation process. Here we confirm and extend this notion showing that, just as different brain activities prepare different voluntary actions, also different brain activities prepare to observe different predictable actions. Videos of two different actions from egocentric point of view were presented in separate blocks: (i) grasping of a cup and (ii) impossible grasping of a cup. Subjects had to passively observe the videos showing object-directed hand movements. Through the use of the event-related potentials, we found a cortical activity before observing the actions, which was very similar to the one recorded prior to the actual execution of that same action, in terms of both topography and latency. This anticipatory activity does not represent a general preparation state but an action-specific state, because being dependent on the specific meaning of the forthcoming action. These results reinforce our knowledge about the correspondence between action, perception and cognition. PMID:25261822

  6. The influence of expertise on brain activation of the action observation network during anticipation of tennis and volleyball serves

    PubMed Central

    Balser, Nils; Lorey, Britta; Pilgramm, Sebastian; Naumann, Tim; Kindermann, Stefan; Stark, Rudolf; Zentgraf, Karen; Williams, A. Mark; Munzert, Jörn

    2014-01-01

    In many daily activities, and especially in sport, it is necessary to predict the effects of others' actions in order to initiate appropriate responses. Recently, researchers have suggested that the action–observation network (AON) including the cerebellum plays an essential role during such anticipation, particularly in sport expert performers. In the present study, we examined the influence of task-specific expertise on the AON by investigating differences between two expert groups trained in different sports while anticipating action effects. Altogether, 15 tennis and 16 volleyball experts anticipated the direction of observed tennis and volleyball serves while undergoing functional magnetic resonance imaging (fMRI). The expert group in each sport acted as novice controls in the other sport with which they had only little experience. When contrasting anticipation in both expertise conditions with the corresponding untrained sport, a stronger activation of AON areas (SPL, SMA), and particularly of cerebellar structures, was observed. Furthermore, the neural activation within the cerebellum and the SPL was linearly correlated with participant's anticipation performance, irrespective of the specific expertise. For the SPL, this relationship also holds when an expert performs a domain-specific anticipation task. Notably, the stronger activation of the cerebellum as well as of the SMA and the SPL in the expertise conditions suggests that experts rely on their more fine-tuned perceptual-motor representations that have improved during years of training when anticipating the effects of others' actions in their preferred sport. The association of activation within the SPL and the cerebellum with the task achievement suggests that these areas are the predominant brain sites involved in fast motor predictions. The SPL reflects the processing of domain-specific contextual information and the cerebellum the usage of a predictive internal model to solve the anticipation

  7. [The activity of prooxidant-antioxidant system in loach embryos under the action of microwave radiation].

    PubMed

    Iaremchuk, M M; Dyka, M V; Sanahurs'kyĭ, D I

    2014-01-01

    Electromagnetic radiation (EMR) affects biological organisms, primarily on the cellular level. However, the effects of EMR at low-intensity exposure on animals and state of metabolic systems are not fully defined yet. Thus, research of microwave radiation influence on the processes of lipid peroxidation and antioxidant protection system is important for understanding the mechanisms of EMR action on the cell, in particular, and organism development on the whole. The content of lipid peroxidation products--lipid hydroperoxides, thiobarbituric acid reactive substances and the activity of antioxidant enzymes--superoxide dismutase, glutathione peroxidase and catalase in loach embryos under the action of microwave radiation (GSM-900 MHz, SAR = 1.1 Vt/kg) lasting 1; 5; 10 and 20 min during early embryogenesis were studied. It has been found that content of lipid peroxidation products in germ cells undergoes significant changes under the action of low-intensity EMR. The effect of microwave radiation (1, 5, 10 min) leads to the increase of superoxide dismutase activity, nevertheless, 20 min exposure decreased this index to the level of control values as it is shown. It has been established that EMR at frequencies used for mobile communications reduce the activity of antioxidant protection system components, especially catalase and glutathione peroxidase. The growth of catalase activity at the 10-cell stage of blastomere division (P < 0.05) is an exception. The results of two-way analysis of variance attest that microwave radiation factor causes the large part of all observable modifications. PMID:25816598

  8. [The activity of prooxidant-antioxidant system in loach embryos under the action of microwave radiation].

    PubMed

    Iaremchuk, M M; Dyka, M V; Sanahurs'kyĭ, D I

    2014-01-01

    Electromagnetic radiation (EMR) affects biological organisms, primarily on the cellular level. However, the effects of EMR at low-intensity exposure on animals and state of metabolic systems are not fully defined yet. Thus, research of microwave radiation influence on the processes of lipid peroxidation and antioxidant protection system is important for understanding the mechanisms of EMR action on the cell, in particular, and organism development on the whole. The content of lipid peroxidation products--lipid hydroperoxides, thiobarbituric acid reactive substances and the activity of antioxidant enzymes--superoxide dismutase, glutathione peroxidase and catalase in loach embryos under the action of microwave radiation (GSM-900 MHz, SAR = 1.1 Vt/kg) lasting 1; 5; 10 and 20 min during early embryogenesis were studied. It has been found that content of lipid peroxidation products in germ cells undergoes significant changes under the action of low-intensity EMR. The effect of microwave radiation (1, 5, 10 min) leads to the increase of superoxide dismutase activity, nevertheless, 20 min exposure decreased this index to the level of control values as it is shown. It has been established that EMR at frequencies used for mobile communications reduce the activity of antioxidant protection system components, especially catalase and glutathione peroxidase. The growth of catalase activity at the 10-cell stage of blastomere division (P < 0.05) is an exception. The results of two-way analysis of variance attest that microwave radiation factor causes the large part of all observable modifications.

  9. Action of Heavy Metals on Hill Activity and O2 Evolution in Anacystis nidulans1

    PubMed Central

    Singh, Devendra P.; Singh, S. P.

    1987-01-01

    Addition of 5 micromolar Cu2+, Cd2+, and Zn2+ was inhibitory to 10 micromolar H2O2-supported Hill activity (dichlorophenolindophenol reduction) and O2 evolution in membrane preparation from Anacystis nidulans. The reversal of Cd2+ and Zn2+ inhibition, in contrast to Cu2+, by exogenously added catalase (EC 1.11.1.6) suggested that the former cations were inhibitory to H2O2 degradation. Ascorbic acid (20 micromolar) supported 27% of the Hill activity which was insensitive to DCMU (10 micromolar) and the remaining activity, attributable to the DCMU sensitive process, was sensitive to inhibition by Cu2+ only. It is suggestive that the action site of Cd2+ and Zn2+ is located between the electron donation sites of H2O2 and ascorbic acid, while that of Cu2+ is located beyond it. Electron donation by reduced glutathione was insensitive to DCMU and Cu2+, indicating that the action site of Cu2+ is prior to its electron donation site. Further, the phenanthroline (10 micromolar) reversal of Cu2+ inhibition of Hill activity suggested a tentative action site of Cu2+ at the level of cytochrome. PMID:16665185

  10. Enhanced activation of motor execution networks using action observation combined with imagination of lower limb movements.

    PubMed

    Villiger, Michael; Estévez, Natalia; Hepp-Reymond, Marie-Claude; Kiper, Daniel; Kollias, Spyros S; Eng, Kynan; Hotz-Boendermaker, Sabina

    2013-01-01

    The combination of first-person observation and motor imagery, i.e. first-person observation of limbs with online motor imagination, is commonly used in interactive 3D computer gaming and in some movie scenes. These scenarios are designed to induce a cognitive process in which a subject imagines himself/herself acting as the agent in the displayed movement situation. Despite the ubiquity of this type of interaction and its therapeutic potential, its relationship to passive observation and imitation during observation has not been directly studied using an interactive paradigm. In the present study we show activation resulting from observation, coupled with online imagination and with online imitation of a goal-directed lower limb movement using functional MRI (fMRI) in a mixed block/event-related design. Healthy volunteers viewed a video (first-person perspective) of a foot kicking a ball. They were instructed to observe-only the action (O), observe and simultaneously imagine performing the action (O-MI), or imitate the action (O-IMIT). We found that when O-MI was compared to O, activation was enhanced in the ventralpremotor cortex bilaterally, left inferior parietal lobule and left insula. The O-MI and O-IMIT conditions shared many activation foci in motor relevant areas as confirmed by conjunction analysis. These results show that (i) combining observation with motor imagery (O-MI) enhances activation compared to observation-only (O) in the relevant foot motor network and in regions responsible for attention, for control of goal-directed movements and for the awareness of causing an action, and (ii) it is possible to extensively activate the motor execution network using O-MI, even in the absence of overt movement. Our results may have implications for the development of novel virtual reality interactions for neurorehabilitation interventions and other applications involving training of motor tasks. PMID:24015241

  11. Enhanced Activation of Motor Execution Networks Using Action Observation Combined with Imagination of Lower Limb Movements

    PubMed Central

    Villiger, Michael; Estévez, Natalia; Hepp-Reymond, Marie-Claude; Kiper, Daniel; Kollias, Spyros S.; Eng, Kynan; Hotz-Boendermaker, Sabina

    2013-01-01

    The combination of first-person observation and motor imagery, i.e. first-person observation of limbs with online motor imagination, is commonly used in interactive 3D computer gaming and in some movie scenes. These scenarios are designed to induce a cognitive process in which a subject imagines himself/herself acting as the agent in the displayed movement situation. Despite the ubiquity of this type of interaction and its therapeutic potential, its relationship to passive observation and imitation during observation has not been directly studied using an interactive paradigm. In the present study we show activation resulting from observation, coupled with online imagination and with online imitation of a goal-directed lower limb movement using functional MRI (fMRI) in a mixed block/event-related design. Healthy volunteers viewed a video (first-person perspective) of a foot kicking a ball. They were instructed to observe-only the action (O), observe and simultaneously imagine performing the action (O-MI), or imitate the action (O-IMIT). We found that when O-MI was compared to O, activation was enhanced in the ventralpremotor cortex bilaterally, left inferior parietal lobule and left insula. The O-MI and O-IMIT conditions shared many activation foci in motor relevant areas as confirmed by conjunction analysis. These results show that (i) combining observation with motor imagery (O-MI) enhances activation compared to observation-only (O) in the relevant foot motor network and in regions responsible for attention, for control of goal-directed movements and for the awareness of causing an action, and (ii) it is possible to extensively activate the motor execution network using O-MI, even in the absence of overt movement. Our results may have implications for the development of novel virtual reality interactions for neurorehabilitation interventions and other applications involving training of motor tasks. PMID:24015241

  12. Exploring the Neural Basis of Real-Life Joint Action: Measuring Brain Activation during Joint Table Setting with Functional Near-Infrared Spectroscopy

    PubMed Central

    Egetemeir, Johanna; Stenneken, Prisca; Koehler, Saskia; Fallgatter, Andreas J.; Herrmann, Martin J.

    2011-01-01

    Many every-day life situations require two or more individuals to execute actions together. Assessing brain activation during naturalistic tasks to uncover relevant processes underlying such real-life joint action situations has remained a methodological challenge. In the present study, we introduce a novel joint action paradigm that enables the assessment of brain activation during real-life joint action tasks using functional near-infrared spectroscopy (fNIRS). We monitored brain activation of participants who coordinated complex actions with a partner sitting opposite them. Participants performed table setting tasks, either alone (solo action) or in cooperation with a partner (joint action), or they observed the partner performing the task (action observation). Comparing joint action and solo action revealed stronger activation (higher [oxy-Hb]-concentration) during joint action in a number of areas. Among these were areas in the inferior parietal lobule (IPL) that additionally showed an overlap of activation during action observation and solo action. Areas with such a close link between action observation and action execution have been associated with action simulation processes. The magnitude of activation in these IPL areas also varied according to joint action type and its respective demand on action simulation. The results validate fNIRS as an imaging technique for exploring the functional correlates of interindividual action coordination in real-life settings and suggest that coordinating actions in real-life situations requires simulating the actions of the partner. PMID:21927603

  13. Kinetic investigation of the action of hyaluronidase on hyaluronan using the Morgan-Elson and neocuproine assays.

    PubMed

    Vercruysse, K P; Lauwers, A R; Demeester, J M

    1995-08-15

    In this paper we describe kinetic investigations of the action of testicular hyaluronidase on hyaluronan. We have compared the use of two spectrophotometric assays, the first based on the Morgan-Elson reaction and the second on the neocuproine reaction. With the neocuproine reaction Km was found to be 0.46 mg/ml and Vmax to be 126 nmol l-1 s-1. Because of a low sensitivity and the production of interfering precipitates, the Morgan-Elson assay cannot be used for kinetic investigation of the enzyme. Furthermore this assay is prone to interference from compounds such as disodium cromoglycate, (+)-catechine, penicillamine, CaCl2 and acetate buffer.

  14. Assessing the Use of YouTube Videos and Interactive Activities as a Critical Thinking Stimulator for Tertiary Students: An Action Research

    ERIC Educational Resources Information Center

    June, Sethela; Yaacob, Aizan; Kheng, Yeoh Khar

    2014-01-01

    The purpose of this action research was to investigate the use of YouTube videos and interactive activities in stimulating critical thinking among students from a public university in Malaysia. There were 50 students of mixed background, comprised of local and foreign students who participated in this study which lasted for one semester. Data was…

  15. Corrective Action Investigation Plan for Corrective Action Unit 322: Areas 1 and 3 Release Sites and Injection Wells, Nevada Test Site, Nevada: Revision 0, Including Record of Technical Change No. 1

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2003-07-16

    This Corrective Action Investigation Plan contains the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office's approach to collect the data necessary to evaluate corrective action alternatives (CAAs) appropriate for the closure of Corrective Action Unit (CAU) 322, Areas 1 and 3 Release Sites and Injection Wells, Nevada Test Site, Nevada, under the Federal Facility Agreement and Consent Order. Corrective Action Unit 322 consists of three Corrective Action Sites (CASs): 01-25-01, AST Release (Area 1); 03-25-03, Mud Plant AST Diesel Release (Area 3); 03-20-05, Injection Wells (Area 3). Corrective Action Unit 322 is being investigated because existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives. The investigation of three CASs in CAU 322 will determine if hazardous and/or radioactive constituents are present at concentrations and locations that could potentially pose a threat to human health and the environment. The results of this field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  16. An investigation of semantic similarity judgments about action and non-action verbs in Parkinson's disease: implications for the Embodied Cognition Framework

    PubMed Central

    Kemmerer, David; Miller, Luke; MacPherson, Megan K.; Huber, Jessica; Tranel, Daniel

    2013-01-01

    The Embodied Cognition Framework maintains that understanding actions requires motor simulations subserved in part by premotor and primary motor regions. This hypothesis predicts that disturbances to these regions should impair comprehension of action verbs but not non-action verbs. We evaluated the performances of 10 patients with Parkinson's disease (PD) and 10 normal comparison (NC) participants on a semantic similarity judgment task (SSJT) that included four classes of action verbs and two classes of non-action verbs. The patients were tested both ON and OFF medication. The most salient results involved the accuracies and reaction times (RTs) for the action verbs taken as a whole and the non-action verbs taken as a whole. With respect to accuracies, the patients did not perform significantly worse than the NC participants for either the action verbs or the non-action verbs, regardless of whether they were ON or OFF their medication. And with respect to RTs, although the patients' responses were significantly slower than those of the NC participants for the action verbs, comparable processing delays were also observed for the non-action verbs; moreover, there was again no notable influence of medication. The major dissociation was therefore not between action and non-action verbs, but rather between accuracies (relatively intact) and RTs (relatively delayed). Overall, the data suggest that semantic similarity judgments for both action and non-action verbs are correct but slow in individuals with PD. These results provide new insights about language processing in PD, and they raise important questions about the explanatory scope of the Embodied Cognition Framework. PMID:23616759

  17. Nurse Activism in the newborn intensive care unit: actions in response to an ethical dilemma.

    PubMed

    Settle, Peggy Doyle

    2014-03-01

    Nurses working in a newborn intensive care unit report that treatment decision disagreements for infants in their care may lead to ethical dilemmas involving all health-care providers. Applying Rest's Four-Component Model of Moral Action as the theoretical framework, this study examined the responses of 224 newborn intensive care unit nurses to the Nurses Ethical Involvement Survey. The three most frequent actions selected were as follows: talking with other nurses, talking with doctors, and requesting a team meeting. The multiple regression analysis indicates that newborn intensive care unit nurses with greater concern for the ethical aspects of clinical practice (p = .001) and an increased perception of their ability to influence ethical decision making (p = .018) were more likely to display Nurse Activism. Future research is necessary to identify other factors leading to and inhibiting Nurse Activism as these findings explained just 8.5% of the variance.

  18. Predicting Changes in Physical Activity among Adolescents: The Role of Self-Efficacy, Intention, Action Planning and Coping Planning

    ERIC Educational Resources Information Center

    Araujo-Soares, Vera; McIntyre, Teresa; Sniehotta, Falko F.

    2009-01-01

    This paper aims to test the direct predictors of the theory of planned behaviour (TPB), action planning and coping planning as predictors of changes in physical activity (PA) in 157 adolescents (mean age: 12). TPB measures, the Action Planning and Coping Planning Scales (APCPS) and the International Physical Activity Questionnaires were measured…

  19. Interpretation of biological activity data of bacterial endotoxins by simple molecular models of mechanism of action.

    PubMed

    Frecer, V; Ho, B; Ding, J L

    2000-02-01

    Lipid A moiety has been identified as the bioactive component of bacterial endotoxins (lipopolysaccharides). However, the molecular mechanism of biological activity of lipid A is still not fully understood. This paper contributes to understanding of the molecular mechanism of action of bacterial endotoxins by comparing molecular modelling results for two possible mechanisms with the underlying experimental data. Mechanisms of action involving specific binding of lipid A to a protein receptor as well as nonspecific intercalation into phospholipid membrane of a host cell were modelled and analysed. As the cellular receptor for endotoxin has not been identified, a model of a peptidic pseudoreceptor was proposed, based on molecular structure, symmetry of the lipid A moiety and the observed character of endotoxin-binding sites in proteins. We have studied the monomeric form of lipid A from Escherichia coli and its seven synthetic analogues with varying numbers of phosphate groups and correlated them with known biological activities determined by the Limulus assay. Gibbs free energies associated with the interaction of lipid A with the pseudoreceptor model and intercalation into phospholipid membrane calculated by molecular mechanics and molecular dynamics methods were used to compare the two possible mechanisms of action. The results suggest that specific binding of lipid A analogues to the peptidic pseudoreceptor carrying an amphipathic cationic binding pattern BHPHB (B, basic; H, hydrophobic; P, polar residue, respectively) is energetically more favourable than intercalation into the phospholipid membrane. In addition, binding affinities of lipid A analogues to the best minimum binding sequence KFSFK of the pseudoreceptor correlated with the experimental Limulus activity parameter. This correlation enabled us to rationalize the observed relationship between the number and position of the phosphate groups in the lipid A moiety and its biological activity in terms of

  20. Analyses of Activity Design in Geometry in the Light of Student Actions.

    ERIC Educational Resources Information Center

    Hadas, Nurit; Hershkowitz, Rina; Schwarz, Baruch B.

    2002-01-01

    Investigates the effects of inquiry activities on a dynamic geometry environment designed to confront students with contradictions and uncertainties. Characterizes epistemological, didactic, and cognitive aspects of the activities. Analyzes the interplay among these three aspects, students' investigations, and their explanations. (Author/KHR)

  1. Ammonium carbamates as highly active transdermal permeation enhancers with a dual mechanism of action.

    PubMed

    Novotný, Michal; Klimentová, Jana; Janůšová, Barbora; Palát, Karel; Hrabálek, Alexandr; Vávrová, Kateřina

    2011-03-10

    Transdermal permeation enhancers are compounds that temporarily increase drug flux through the skin by interacting with constituents of the stratum corneum. Transkarbam 12 (T12) is a highly active, broad-spectrum, biodegradable enhancer with low toxicity and low dermal irritation. We show here that T12 acts by a dual mechanism of action. The first part of this activity is associated with its ammonium carbamate polar head as shown by its pH-dependent effects on the permeation of two model drugs. Once this ammonium carbamate penetrates into the stratum corneum intercellular lipids, it rapidly decomposes releasing two molecules of protonated dodecyl 6-aminohexanoate (DDEAC) and carbon dioxide. This was observed by thermogravimetric analysis and infrared spectroscopy. This step of T12 action influences drug permeation through lipidic pathways, not through the aqueous pores (polar pathway) as shown by its effects on various model drugs and electrical impedance. Consequently, protonated DDEAC released in the stratum corneum is also an active enhancer. It broadens the scope of T12 action since it is also able to increase permeation of hydrophilic drugs that prefer the pore pathway. Thus, this dual effect of T12 is likely responsible for its favorable properties, which make it a good candidate for prospective clinical use.

  2. Mode of Action of Lactoperoxidase as Related to Its Antimicrobial Activity: A Review

    PubMed Central

    Bafort, F.; Parisi, O.; Perraudin, J.-P.; Jijakli, M. H.

    2014-01-01

    Lactoperoxidase is a member of the family of the mammalian heme peroxidases which have a broad spectrum of activity. Their best known effect is their antimicrobial activity that arouses much interest in in vivo and in vitro applications. In this context, the proper use of lactoperoxidase needs a good understanding of its mode of action, of the factors that favor or limit its activity, and of the features and properties of the active molecules. The first part of this review describes briefly the classification of mammalian peroxidases and their role in the human immune system and in host cell damage. The second part summarizes present knowledge on the mode of action of lactoperoxidase, with special focus on the characteristics to be taken into account for in vitro or in vivo antimicrobial use. The last part looks upon the characteristics of the active molecule produced by lactoperoxidase in the presence of thiocyanate and/or iodide with implication(s) on its antimicrobial activity. PMID:25309750

  3. The action of the canine diaphragm on the lower ribs depends on activation.

    PubMed

    De Troyer, André

    2011-11-01

    Conventional wisdom maintains that the diaphragm lifts the lower ribs during isolated contraction. Recent studies in dogs have shown, however, that supramaximal, tetanic stimulation of the phrenic nerves displaces the lower ribs caudally and inward. In the present study, the hypothesis was tested that the action of the canine diaphragm on these ribs depends on the magnitude of muscle activation. Two experiments were performed. In the first, the C5 and C6 phrenic nerve roots were selectively stimulated in 6 animals with the airway occluded, and the level of diaphragm activation was altered by adjusting the stimulation frequency. In the second experiment, all the inspiratory intercostal muscles were severed in 7 spontaneously breathing animals, so that the diaphragm was the only muscle active during inspiration, and neural drive was increased by a succession of occluded breaths. The changes in airway opening pressure and the craniocaudal displacements of ribs 5 and 10 were measured in each animal. The data showed that 1) contraction of the diaphragm causes the upper ribs to move caudally; 2) during phrenic nerve stimulation, the lower ribs move cranially when the level of diaphragm activation is low, but they move caudally when the level of muscle activation is high and the entire rib cage is exposed to pleural pressure; and 3) during spontaneous diaphragm contraction, however, the lower ribs always move cranially, even when neural drive is elevated and the change in pleural pressure is large. It is concluded that the action of the diaphragm on the lower ribs depends on both the magnitude and the mode of muscle activation. These findings can reasonably explain the apparent discrepancies between previous studies. They also imply that observations made during phrenic nerve stimulation do not necessarily reflect the physiological action of the diaphragm.

  4. Institutional Diversity in Collective Action: Investigating Successful Village Level Maintenance of Hand Pumps in Malawi

    NASA Astrophysics Data System (ADS)

    Joubert, Brian Anthony

    Providing clean water to rural communities in sub-Saharan Africa remains a challenge. Unsanitary and distant water sources cause a host of health and humanitarian problems. A common means of remedying this situation has been the donation of improved water sources, fitted with low-cost hand pumps. Due donor capacity and/ or policy most hand pumps are donated under the guise of Village Level Operation and Maintenance (VLOM). This premises the notion that recipient communities will take ownership of the new pump and as such will ensure its maintenance. To assist with this many donors carry out programs of technical repair training and the structuring of in-village leadership and management groups. The reality is that a high proportion of these pumps break down after donation and cease to work thereafter. Measures to redress technical elements of these failures through increased training or adequate distribution of spares has seen some success but failure rates remains high. This has led to a call for more attention to demand side issues, focusing on the communal aspects that may influence a village to act collectively in the maintenance of its hand pump. This thesis researched five Malawian villages where the community had maintained their hand pumps for a period of 10 or more years. These hand pumps were treated as shared resources and the literature on common-pool resources and social institutions was used as a theoretical framework. Applying these theories proved to be appropriate for analyzing the norms, conventions and forms of cooperative conduct. This allowed the research to gain insights into institutional diversity and the relationship between 'formal institutions', most often exogenous in nature, and informal' or customary collective action institutions embedded within the communities. Findings showed the emergence of three predominant themes within these successful case studies: 1) the role of leadership at varying levels and how it is embodied

  5. Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax

    PubMed Central

    2014-01-01

    Polysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extracts of thermophilic microorganisms (Thermotoga neapolitana, Geobacillus thermantarcticus and Thermoanaerobacterium thermostercoris) is studied using as substrate hemicellulose from one of the most interesting biomass crops, the giant reed (Arundo donax L.). This biomass can be cultivated without competition and a huge amount of rhizomes remains in the soil at the end of cropping cycle (10–15 years) representing a further source of useful molecules. Optimization of the procedure for preparation of the hemicellulose fraction from rhizomes of Arundo donax, is studied. Polysaccharidases from crude extracts of thermophilic microorganisms revealed to be suitable for total degradative action and/or production of small useful oligosaccharides from hemicelluloses from A. donax. Xylobiose and interesting tetra- and pentasaccharide are obtained by enzymatic action in different conditions. Convenient amount of raw material was processed per mg of crude enzymes. Raw hemicelluloses and pretreated material show antioxidant activity unlike isolated tetra- and pentasaccharide. The body of results suggest that rhizomes represent a useful raw material for the production of valuable industrial products, thus allowing to increase the economic efficiency of A. donax cultivation. PMID:25024928

  6. Antischistosomal Activity of Trioxaquines: In Vivo Efficacy and Mechanism of Action on Schistosoma mansoni

    PubMed Central

    Portela, Julien; Boissier, Jérôme; Gourbal, Benjamin; Pradines, Vincent; Collière, Vincent; Coslédan, Frédéric; Meunier, Bernard; Robert, Anne

    2012-01-01

    Schistosomiasis is among the most neglected tropical diseases, since its mode of spreading tends to limit the contamination to people who are in contact with contaminated waters in endemic countries. Here we report the in vitro and in vivo anti-schistosomal activities of trioxaquines. These hybrid molecules are highly active on the larval forms of the worms and exhibit different modes of action, not only the alkylation of heme. The synergy observed with praziquantel on infected mice is in favor of the development of these trioxaquines as potential anti-schistosomal agents. PMID:22348155

  7. Addressing bioterrorism concerns: options for investigating the mechanism of action of Staphylococcus aureus enterotoxin B.

    PubMed

    Lindsay, C D; Griffiths, G D

    2013-06-01

    Staphylococcal enterotoxin B (SEB) is of concern to military and civilian populations as a bioterrorism threat agent. It is a highly potent toxin produced by Staphylococcus aureus and is stable in storage and under aerosolisation; it is able to produce prolonged highly incapacitating illness at very low-inhaled doses and death at elevated doses. Concerns regarding SEB are compounded by the lack of effective medical countermeasures for mass treatment of affected populations. This article considers the mechanism of action of SEB, the availability of appropriate experimental models for evaluating the efficacy of candidate medical countermeasures with particular reference to the need to realistically model SEB responses in man and the availability of candidate countermeasures (with an emphasis on commercial off-the-shelf options). The proposed in vitro approaches would be in keeping with Dstl’s commitment to reduction, refinement and replacement of animal models in biomedical research, particularly in relation to identifying valid alternatives to the use of nonhuman primates in experimental studies.

  8. Landowner's perception of flood risk and preventive actions in estuarine environment: An empirical investigation.

    PubMed

    Rambonilaza, Tina; Joalland, Olivier; Brahic, Elodie

    2016-09-15

    Within Europe, flood and coastal risk management is undergoing a major paradigm shift as it moves from an approach dominated by investment in flood defence and control infrastructure to another one in which non-structural measures are favoured. One research challenge consists in developing a better understanding of local population risk perception and its effects on prevention and preparedness actions in order to improve social acceptability of adaptive flood risk management. Landowners' involvement in wetland management offer benefits beyond the line of their property. Accordingly, the purpose of this study is to achieve an empirical understanding of risk perception and self-protective behaviour among the landowners of the riparian marshes in the Gironde Estuary, in France. Application of the psychometric approach reveals that flood risk perception among landowners can be characterised by three synthetic variables that indicate on the degree of exposure, the sense of control and knowledge of the risk. Examining the relationships between these perceived risk dimensions and landowners' participation in water structures management provides three profiles of self-protective behaviour distinguishing "vulnerable", "autonomous", and "passive" individuals. Finally, implications of our findings for the management of flood risk in estuarine environment which is often drained areas are discussed.

  9. Drug-set enrichment analysis: a novel tool to investigate drug mode of action

    PubMed Central

    Napolitano, Francesco; Sirci, Francesco; Carrella, Diego; di Bernardo, Diego

    2016-01-01

    Motivation: Automated screening approaches are able to rapidly identify a set of small molecules inducing a desired phenotype from large small-molecule libraries. However, the resulting set of candidate molecules is usually very diverse pharmacologically, thus little insight on the shared mechanism of action (MoA) underlying their efficacy can be gained. Results: We introduce a computational method (Drug-Set Enrichment Analysis—DSEA) based on drug-induced gene expression profiles, which is able to identify the molecular pathways that are targeted by most of the drugs in the set. By diluting drug-specific effects unrelated to the phenotype of interest, DSEA is able to highlight phenotype-specific pathways, thus helping to formulate hypotheses on the MoA shared by the drugs in the set. We validated the method by analysing five different drug-sets related to well-known pharmacological classes. We then applied DSEA to identify the MoA shared by drugs known to be partially effective in rescuing mutant cystic fibrosis transmembrane conductance regulator (CFTR) gene function in Cystic Fibrosis. Availability and implementation: The method is implemented as an online web tool publicly available at http://dsea.tigem.it. Contact: dibernardo@tigem.it Supplementary information: Supplementary data are available at Bioinformatics online. PMID:26415724

  10. Landowner's perception of flood risk and preventive actions in estuarine environment: An empirical investigation.

    PubMed

    Rambonilaza, Tina; Joalland, Olivier; Brahic, Elodie

    2016-09-15

    Within Europe, flood and coastal risk management is undergoing a major paradigm shift as it moves from an approach dominated by investment in flood defence and control infrastructure to another one in which non-structural measures are favoured. One research challenge consists in developing a better understanding of local population risk perception and its effects on prevention and preparedness actions in order to improve social acceptability of adaptive flood risk management. Landowners' involvement in wetland management offer benefits beyond the line of their property. Accordingly, the purpose of this study is to achieve an empirical understanding of risk perception and self-protective behaviour among the landowners of the riparian marshes in the Gironde Estuary, in France. Application of the psychometric approach reveals that flood risk perception among landowners can be characterised by three synthetic variables that indicate on the degree of exposure, the sense of control and knowledge of the risk. Examining the relationships between these perceived risk dimensions and landowners' participation in water structures management provides three profiles of self-protective behaviour distinguishing "vulnerable", "autonomous", and "passive" individuals. Finally, implications of our findings for the management of flood risk in estuarine environment which is often drained areas are discussed. PMID:27240203

  11. Zoonoses action plan Salmonella monitoring programme: an investigation of the sampling protocol.

    PubMed

    Snary, E L; Munday, D K; Arnold, M E; Cook, A J C

    2010-03-01

    The Zoonoses Action Plan (ZAP) Salmonella Programme was established by the British Pig Executive to monitor Salmonella prevalence in quality-assured British pigs at slaughter by testing a sample of pigs with a meat juice enzyme-linked immunosorbent assay for antibodies against group B and C(1) Salmonella. Farms were assigned a ZAP level (1 to 3) depending on the monitored prevalence, and ZAP 2 or 3 farms were required to act to reduce the prevalence. The ultimate goal was to reduce the risk of human salmonellosis attributable to British pork. A mathematical model has been developed to describe the ZAP sampling protocol. Results show that the probability of assigning a farm the correct ZAP level was high, except for farms that had a seroprevalence close to the cutoff points between different ZAP levels. Sensitivity analyses identified that the probability of assigning a farm to the correct ZAP level was dependent on the sensitivity and specificity of the test, the number of batches taken to slaughter each quarter, and the number of samples taken per batch. The variability of the predicted seroprevalence was reduced as the number of batches or samples increased and, away from the cutoff points, the probability of being assigned the correct ZAP level increased as the number of batches or samples increased. In summary, the model described here provided invaluable insight into the ZAP sampling protocol. Further work is required to understand the impact of the program for Salmonella infection in British pig farms and therefore on human health.

  12. Corrective Action Investigation Plan for Corrective Action Unit 486: Double Tracks RADSAFE Ares, Nellis Air Force Range, Nevada, Rev. 0; DOE/NV--523 UPDATED WITH ROTC No.1 and 2

    SciTech Connect

    ITLV

    1999-11-16

    This CAIP presents a plan to investigate the DTRSA where unregulated disposal of radioactive and possibly hazardous waste occurred during decontamination activities for the Double Tracks test. The purpose of the corrective action investigation described in this CAIP is to: Identify and verify the locations of the decontamination facility and animal burial pit within the DTRSA; Identify the presence and nature of COPCs; Determine the vertical and lateral extent of COPCs; and Provide sufficient information and data to develop and evaluate appropriate corrective actions for the CAS. This CAIP was developed using the U.S. Environmental Protection Agency (EPA) Data Quality Objectives (DQOs) (EPA, 1994d) process to clearly define the goals for collecting environmental data, to determine data uses, and to design a data collection program that will satisfy these uses. A DQO scoping meeting was held prior to preparation of this plan; a brief summary of the DQOs is presented in Section 3.4. A more detailed summary of the DQO process and results is included in Appendix A.

  13. Experimental investigation of active loads control for aircraft landing gear

    NASA Technical Reports Server (NTRS)

    Mcgehee, J. R.; Dreher, R. C.

    1982-01-01

    Aircraft dynamic loads and vibrations resulting from landing impact and from runway and taxiway unevenness are recognized as significant in causing fatigue damage, dynamic stress on the airframe, crew and passenger discomfort, and reduction of the pilot's ability to control the aircraft during ground operations. One potential method for improving operational characteistics of aircraft on the ground is the application of active control technology to the landing gears to reduce ground loads applied to the airframe. An experimental investigation was conducted which simulated the landing dynamics of a light airplane to determine the feasibility and potential of a series hydraulic active control main landing gear. The experiments involved a passive gear and an active control gear. Results of this investigation show that a series hydraulically controlled gear is feasible and that such a gear is very effective in reducing the loads transmitted by the gear to the airframe during ground operations.

  14. Complex Dynamic Thresholds and Generation of the Action Potentials in the Neural-Activity Model

    NASA Astrophysics Data System (ADS)

    Kirillov, S. Yu.; Nekorkin, V. I.

    2016-05-01

    This work is devoted to studying the processes of activation of the neurons whose excitation thresholds are not constant and vary in time (the so-called dynamic thresholds). The neuron dynamics is described by the FitzHugh-Nagumo model with nonlinear behavior of the recovery variable. The neuron response to the external pulsed activating action in the presence of a slowly varying synaptic current is studied within the framework of this model. The structure of the dynamic threshold is studied and its properties depending on the external-action parameters are established. It is found that the formation of the "folds" in the separatrix threshold manifold in the model phase space is a typical feature of the complex dynamic threshold. High neuron sensitivity to the action of the comparatively weak slow control signals is established. This explains the capability of the neurons to perform flexible tuning of their selective properties for detecting various external signals in sufficiently short times (of the order of duration of several spikes).

  15. Active Drumming Experience Increases Infants’ Sensitivity to Audiovisual Synchrony during Observed Drumming Actions

    PubMed Central

    Timmers, Renee; Hunnius, Sabine

    2015-01-01

    In the current study, we examined the role of active experience on sensitivity to multisensory synchrony in six-month-old infants in a musical context. In the first of two experiments, we trained infants to produce a novel multimodal effect (i.e., a drum beat) and assessed the effects of this training, relative to no training, on their later perception of the synchrony between audio and visual presentation of the drumming action. In a second experiment, we then contrasted this active experience with the observation of drumming in order to test whether observation of the audiovisual effect was as effective for sensitivity to multimodal synchrony as active experience. Our results indicated that active experience provided a unique benefit above and beyond observational experience, providing insights on the embodied roots of (early) music perception and cognition. PMID:26111226

  16. Active Drumming Experience Increases Infants' Sensitivity to Audiovisual Synchrony during Observed Drumming Actions.

    PubMed

    Gerson, Sarah A; Schiavio, Andrea; Timmers, Renee; Hunnius, Sabine

    2015-01-01

    In the current study, we examined the role of active experience on sensitivity to multisensory synchrony in six-month-old infants in a musical context. In the first of two experiments, we trained infants to produce a novel multimodal effect (i.e., a drum beat) and assessed the effects of this training, relative to no training, on their later perception of the synchrony between audio and visual presentation of the drumming action. In a second experiment, we then contrasted this active experience with the observation of drumming in order to test whether observation of the audiovisual effect was as effective for sensitivity to multimodal synchrony as active experience. Our results indicated that active experience provided a unique benefit above and beyond observational experience, providing insights on the embodied roots of (early) music perception and cognition. PMID:26111226

  17. Active learning in forensic science using Brownfield Action in a traditional or hybrid course in earth, environmental, or engineering sciences

    NASA Astrophysics Data System (ADS)

    Bower, P.; Liddicoat (2), J.

    2009-04-01

    Brownfield Action (BA - http://www.brownfieldaction.org) is a web-based, interactive, three-dimensional digital space and learning simulation in which students form geotechnical consulting companies and work collaboratively to explore and solve problems in environmental forensics. BA is being used in the United States at 10 colleges and universities in earth, environmental, or engineering sciences undergraduate and graduate courses. As a semester-long activity or done in modular form for specific topics, BA encourages active learning that requires attention to detail, intuition, and positive interaction between peers that results in Phase 1 and Phase 2 Environmental Site Assessments. Besides use in higher education courses, BA also can be adapted for instruction to local, state, and federal governmental employees, and employees in industry where brownfields need to be investigated or require remediation.

  18. The effects of subjective time pressure and individual differences on hypotheses generation and action prioritization in police investigations.

    PubMed

    Alison, Laurence; Doran, Bernadette; Long, Matthew L; Power, Nicola; Humphrey, Amy

    2013-03-01

    When individuals perceive time pressure, they decrease the generation of diagnostic hypotheses and prioritize information. This article examines whether individual differences in (a) internal time urgency, (b) experience, and (c) fluid mental ability can moderate these effects. Police officers worked through a computer-based rape investigative scenario, in which 35 were subjected to a time pressure manipulation, with their hypotheses generation and prioritization skills compared with a control (n = 41). Group 1 was told they would "get less time to complete the scenario compared with other officers," although both groups had equal amounts of time. Regression analyses found that time pressure reduced hypothesis generation and that individual differences in time urgency moderated this effect; individuals who tend to perceive time to pass more slowly than it is continued to generate hypotheses despite the presence of time pressure. Time pressure also influenced the likelihood of action prioritization at the start of the investigation. Time pressure was found to increase action prioritization, but only for officers with low time urgency or high fluid ability. Experience had no effect on time pressure during the investigative scenario. Implications of these findings are discussed. PMID:23544477

  19. The effects of subjective time pressure and individual differences on hypotheses generation and action prioritization in police investigations.

    PubMed

    Alison, Laurence; Doran, Bernadette; Long, Matthew L; Power, Nicola; Humphrey, Amy

    2013-03-01

    When individuals perceive time pressure, they decrease the generation of diagnostic hypotheses and prioritize information. This article examines whether individual differences in (a) internal time urgency, (b) experience, and (c) fluid mental ability can moderate these effects. Police officers worked through a computer-based rape investigative scenario, in which 35 were subjected to a time pressure manipulation, with their hypotheses generation and prioritization skills compared with a control (n = 41). Group 1 was told they would "get less time to complete the scenario compared with other officers," although both groups had equal amounts of time. Regression analyses found that time pressure reduced hypothesis generation and that individual differences in time urgency moderated this effect; individuals who tend to perceive time to pass more slowly than it is continued to generate hypotheses despite the presence of time pressure. Time pressure also influenced the likelihood of action prioritization at the start of the investigation. Time pressure was found to increase action prioritization, but only for officers with low time urgency or high fluid ability. Experience had no effect on time pressure during the investigative scenario. Implications of these findings are discussed.

  20. Tools to investigate how interprofessional education activities link to competencies.

    PubMed

    West, Courtney; Veronin, Michael; Landry, Karen; Kurz, Terri; Watzak, Bree; Quiram, Barbara; Graham, Lori

    2015-01-01

    Integrating interprofessional education (IPE) activities and curricular components in health professions education has been emphasized recently by the inclusion of accreditation standards across disciplines. The Interprofessional Education Collaborative (IPEC) established IPE competencies in 2009, but evaluating how activities link to competencies has not been investigated in depth. The purpose of this project is to investigate how well two IPE activities align with IPEC competencies. To evaluate how our IPE activities met IPEC competencies, we developed a checklist and an observation instrument. A brief description of each is included as well as the outcomes. We analyzed Disaster Day, a simulation exercise that includes participants from Nursing, Medicine, and Pharmacy, and Interprofessional Healthcare Ethics (IPHCE), a course that introduced medical, nursing, and pharmacy students to ethical issues using didactic sessions and case discussions. While both activities appeared to facilitate the development of IPE competencies, Disaster Day aligned more with IPEC competencies than the IPHCE course and appears to be a more comprehensive way of addressing IPEC competencies. However, offering one IPE activity or curricular element is not sufficient. Having several IPE options available, utilizing the tools we developed to map the IPE curriculum and evaluating competency coverage is recommended.

  1. Can the Identity of a Behavior Setting Be Perceived Through Patterns of Joint Action? An Investigation of Place Perception

    PubMed Central

    Heft, Harry; Hoch, Justine; Edmunds, Trent; Weeks, Jillian

    2014-01-01

    “Behavior settings” are generated by joint actions of individuals in conjunction with the milieu features (or affordances) that are available. The reported research explores the hypothesis that the identity or meaning of a behavior setting can be perceived by means of the patterns of action collectively generated by the setting’s participants. A set of computer animations was created based on detailed observation of activities in everyday settings. Three experiments were conducted to assess whether perceivers could extract “structure from motion” (in this case, collective actions) that was specific to the particular behavior setting displayed by way of the animations. Two experiments assessed whether individuals could accurately perceive the identity of the behavior settings with such displays, and a third experiment indirectly examined this possibility by evaluating whether setting possibilities and constraints were recognized. The results offered some support for the hypothesis, and suggested several refinements in how to conceptualize a typology of behavior settings. An ecological approach to place perception is also discussed. PMID:25431443

  2. Ground-penetrating radar investigation of St. Leonard's Crypt under the Wawel Cathedral (Cracow, Poland) - COST Action TU1208

    NASA Astrophysics Data System (ADS)

    Benedetto, Andrea; Pajewski, Lara; Dimitriadis, Klisthenis; Avlonitou, Pepi; Konstantakis, Yannis; Musiela, Małgorzata; Mitka, Bartosz; Lambot, Sébastien; Żakowska, Lidia

    2016-04-01

    "Applications of Ground Penetrating Radar in urban areas: the sensitive case of historical cities." The Action TU1208 is coordinated by "Roma Tre University" (Rome, Italy) and the TS was hosted by the Cracow University of Technology (Cracow, Poland). It was attended by 25 PhD students and early-career investigators coming from Albania, Belgium, Germany, Italy, Poland, Romania, Russia and Slovenia. Trainers and Trainees had the great honour and privilege to carry out practical sessions in St Leonard's Crypt, in cooperation with the companies Restauro (Toruń, Poland) and Geoservice (Athens, Greece). Over the centuries, city centres have been continuously changing, developing and adapting to the requirements of society, architectural planning and advancing technology. Under the pressure of urbanisation, many cities and towns have significantly expanded and the limited space in their centres has been exploited more intensively. The shallow subsurface of historical cities is nowadays a very complicated scenario including reams of pipes, cables, rubble, bars and slabs of reinforced concrete, backfilled excavation trenches and pits, cellars, wells, cavities, tunnels, graves, walls and foundations of former houses, churches, monasteries, town fortifications, along with several other modern and ancient structures and manufacts. For the prospection of such a diversified, multilayered, intricate and complex underground environment, both for archaeological and civil-engineering purposes, Ground Penetrating Radar (GPR) is a very effective non-destructive geophysical method. GPR is a powerful tool not only for the prospection of subsurface but also for the non-invasive testing of historical buildings, fountains, historical bridges, sculptures, frescoes, pottery and other objects collected in museums: it can give information about their state of preservation, it can significantly help to address a restoration project properly, and sometimes it can also help to achieve information of

  3. Investigation of the process of ferrite formation in the Li2CO3-ZnO-Fe2O3 system under high-energy actions

    NASA Astrophysics Data System (ADS)

    Surzhikov, A. P.; Lysenko, E. N.; Vlasov, V. A.; Malyshev, A. V.; Nikolaev, E. V.

    2013-11-01

    Results of synthesis of lithium-zinc ferrites under high-energy actions including mechanical activation of the mixture of initial reagents in a planetary mill and heating of reaction mixtures upon exposure to accelerated high-energy electron beams are presented. The initial structure of the reaction mixtures corresponds to the stoichiometric spinel formula: Li0.5(1- x)Zn x Fe2.5-0.5 x О4, where х = 0.2. To elucidate the effect of radiation on kinetic transformations of the phase composition, analogous investigations are performed with thermal annealing in a furnace. It is established that mechanical activation and radiation-thermal heating of the Li2CO3-ZnO-Fe2O3 mixture of initial reagents increases significantly the reactivity of solid phase systems thereby decreasing strongly the temperature of synthesis and improving the homogeneity of the end product. To synthesize Li0.4Fe2.4Zn0.2O4 lithium-zinc ferrospinels with homogeneous composition, it is suffice to provide a synthesis temperature of 600°С with holding time of 60 min under complex high-energy action including mechanical activation of the initial mixture of reagents and heating upon exposure to a high-energy beam of electrons.

  4. Opposing actions of angiopoietin-2 on Tie2 signaling and FOXO1 activation.

    PubMed

    Kim, Minah; Allen, Breanna; Korhonen, Emilia A; Nitschké, Maximilian; Yang, Hee Won; Baluk, Peter; Saharinen, Pipsa; Alitalo, Kari; Daly, Christopher; Thurston, Gavin; McDonald, Donald M

    2016-09-01

    Angiopoietin-2 (ANG2) regulates blood vessel remodeling in many pathological conditions through differential effects on Tie2 signaling. While ANG2 competes with ANG1 to inhibit Tie2, it can paradoxically also promote Tie2 phosphorylation (p-Tie2). A related paradox is that both inactivation and overactivation of Tie2 can result in vascular remodeling. Here, we reconciled these opposing actions of ANG2 by manipulating conditions that govern its actions in the vasculature. ANG2 drove vascular remodeling during Mycoplasma pulmonis infection by acting as a Tie2 antagonist, which led to p-Tie2 suppression, forkhead box O1 (FOXO1) activation, increased ANG2 expression, and vessel leakiness. These changes were exaggerated by anti-Tie2 antibody, inhibition of PI3K signaling, or ANG2 overexpression and were reduced by anti-ANG2 antibody or exogenous ANG1. In contrast, under pathogen-free conditions, ANG2 drove vascular remodeling by acting as an agonist, promoting high p-Tie2, low FOXO1 activation, and no leakage. Tie1 activation was strong under pathogen-free conditions, but infection or TNF-α led to Tie1 inactivation by ectodomain cleavage and promoted the Tie2 antagonist action of ANG2. Together, these data indicate that ANG2 activation of Tie2 supports stable enlargement of normal nonleaky vessels, but reduction of Tie1 in inflammation leads to ANG2 antagonism of Tie2 and initiates a positive feedback loop wherein FOXO1-driven ANG2 expression promotes vascular remodeling and leakage. PMID:27548529

  5. Investigating magnetic activity of F stars with the Kepler mission

    NASA Astrophysics Data System (ADS)

    Mathur, S.; García, R. A.; Ballot, J.; Ceillier, T.; Salabert, D.; Metcalfe, T. S.; Régulo, C.; Jiménez, A.; Bloemen, S.

    2014-08-01

    The dynamo process is believed to drive the magnetic activity of stars like the Sun that have an outer convection zone. Large spectroscopic surveys showed that there is a relation between the rotation periods and the cycle periods: the longer the rotation period is, the longer the magnetic activity cycle period will be. We present the analysis of F stars observed by Kepler for which individual p modes have been measure and with surface rotation periods shorter than 12 days. We defined magnetic indicators and proxies based on photometric observations to help characterise the activity levels of the stars. With the Kepler data, we investigate the existence of stars with cycles (regular or not), stars with a modulation that could be related to magnetic activity, and stars that seem to show a flat behaviour.

  6. An Initial Investigation of Active Galaxies in RESOLVE and ECO

    NASA Astrophysics Data System (ADS)

    Norman, Dara J.; Kannappan, Sheila; Bittner, Ashley; Yarber, Aara'L.; Hoversten, Erik A.; Stark, David; RESOLVE Team

    2016-01-01

    The volume-limited REsolved Spectroscopy Of a Local VolumE (RESOLVE) survey and its complementary Environmental COntext (ECO) catalog are dominated by low mass, gas-rich galaxies, as is typical of the bulk of large-scale structure in the local universe. These surveys, therefore, provide an excellent opportunity to investigate the complete large-scale environments of low-redshift AGN and nuclear starbursts in such galaxies, in order to search for external triggering, examine activity in relation to gas supply, and investigate the role of feedback. By data-mining multi-wavelength catalogs that use varied techniques, we identify known AGN in RESOLVE and ECO, including a population of gas-dominated low-mass galaxies. We take advantage of these surveys' multi-wavelength supporting data to investigate triggering, feedback, and the roles of environment and gas supply in this initial sample of active galaxies. Because biases in standard AGN candidate selection techniques (e.g. BPT, X-ray luminosity) make them individually poor selectors of AGN activity in star-forming and low mass (<10^10 Msun) host galaxies, we also seek to improve the identification of nuclear activity in such galaxies via combined analysis of star formation and AGN signatures. RESOLVE is supported by NSF grant AST-0955368

  7. Menthol shares general anesthetic activity and sites of action on the GABA(A) receptor with the intravenous agent, propofol.

    PubMed

    Watt, Erin E; Betts, Brooke A; Kotey, Francesca O; Humbert, Danielle J; Griffith, Theanne N; Kelly, Elizabeth W; Veneskey, Kelley C; Gill, Nikhila; Rowan, Kathleen C; Jenkins, Andrew; Hall, Adam C

    2008-08-20

    Menthol and related compounds were investigated for modulation of recombinant human gamma-aminobutyric acid type A (GABA(A), alpha(1)beta(2)gamma(2s)) receptor currents expressed in Xenopus oocytes. Sub-maximal (EC(20)) GABA currents were typically enhanced by co-applications of 3-300 microM (+)-menthol (e.g. by approximately 2-fold at 50 microM) > isopulegol > isomenthol> alpha-terpineol > cyclohexanol. We studied menthol's actions on GABA(A) receptors compared to sedatives (benzodiazepines) and intravenous anesthetics (barbiturates, steroids, etomidate and propofol). Flumazenil (a benzodiazepine antagonist) did not inhibit menthol enhancements while currents directly activated by 50 microM propofol were significantly inhibited (by 26+/-3%) by 50 microM (+)-menthol. GABA(A) receptors containing beta(2) subunits with either a point mutation in a methionine residue to a tryptophan at the 286 position (in transmembrane domain 3, TM-3) or a tyrosine to a tryptophan at the 444 position (TM-4) are insensitive to modulation by propofol. Enhancements of GABA EC(20) currents by menthol were equally abolished in GABA(A) alpha(1)beta(2)(M286W)gamma(2s) and alpha(1)beta(2)(Y444W)gamma(2s) receptors while positive modulations by benzodiazepines, barbiturates and steroids were unaffected. Menthol may therefore exert its actions on GABA(A) receptors via sites distinct from benzodiazepines, steroids and barbiturates, and via sites important for modulation by propofol. Finally, using an in vivo tadpole assay, addition of (+)-menthol resulted in a loss of righting reflex with an EC(50) of 23.5+/-4.7 microM (approximately10-fold less potent anesthesia than propofol). Thus, menthol and analogs share general anesthetic action with propofol, possibly via action at similar sites on the GABA(A) receptor. PMID:18593637

  8. Investigation of Antiulcer and Antioxidant Activity of Moclobemide in Rats

    PubMed Central

    Albayrak, Abdulmecit; Alp, Hamit H.; Suleyman, Halis

    2015-01-01

    Objective: Even though there are many drugs for the treatment of gastric ulcers, these drugs sometimes cannot succeed. Since the 1950s, antidepressant drugs have been used for several non-psychiatric indications. Many antidepressant drugs have been shown experimentally to produce antiulcer activity in various ulcer models. Moclobemide is an antidepressant drug which inhibits monoamine oxidase-A (MAO) enzyme selectively. When it is compared to the classic antidepressants drugs, moclobemide is the first choice in depression treatment because of its effectiveness and less side effects. This study aimed to investigate the antiulcer effects of moclobemide and to determine its relationship with antioxidant mechanisms in rat gastric tissue. Materials and Methods: The antiulcer activities of 10, 20, 40, 80, 150 mg/kg moclobemide and 20 mg/kg famotidine have been investigated on indomethacin-induced ulcers in rats, and the results have been compared with that of the control group. Results: Moclobemide decreased the indomethacin-induced ulcers significantly at all doses used. While used doses of moclobemide increased the glutathione (GSH), nitric oxide (NO) level and superoxide dismutase (SOD) activity, it decreased the malondialdehyde (MDA) level and myeloperoxidase (MPO) activity in stomach tissue when compared to the control group. Conclusion: It is determined that an antidepressant drug, moclobemide is a potent anti-ulcer agent. Inhibition of toxic oxidant radicals and activation of antioxidant mechanisms play a role in its anti-ulcer effect mechanisms. PMID:25745343

  9. Students' Performance in Investigative Activity and Their Understanding of Activity Aims

    ERIC Educational Resources Information Center

    Gomes, Alessandro Damasio Trani; Borges, A. Tarciso; Justi, Rosaria

    2008-01-01

    This study investigates the relationship between the students' understanding of the aims of an investigative activity and their performance when conducting it. One hundred and eighty-one year nine students from a public middle school in Brazil took part in the study. Students working in pairs were asked to investigate two problems using a…

  10. Antiviral activity and specific modes of action of bacterial prodigiosin against Bombyx mori nucleopolyhedrovirus in vitro.

    PubMed

    Zhou, Wei; Zeng, Cheng; Liu, RenHua; Chen, Jie; Li, Ru; Wang, XinYan; Bai, WenWen; Liu, XiaoYuan; Xiang, TingTing; Zhang, Lin; Wan, YongJi

    2016-05-01

    Prodigiosin, the tripyrrole red pigment, is a bacterial secondary metabolite with multiple bioactivities; however, the antiviral activity has not been reported yet. In the present study, we found the antiviral activity of bacterial prodigiosin on Bombyx mori nucleopolyhedrovirus (BmNPV)-infected cells in vitro, with specific modes of action. Prodigiosin at nontoxic concentrations selectively killed virus-infected cells, inhibited viral gene transcription, especially viral early gene ie-1, and prevented virus-mediated membrane fusion. Under prodigiosin treatment, both progeny virus production and viral DNA replication were significantly inhibited. Fluorescent assays showed that prodigiosin predominantly located in cytoplasm which suggested it might interact with cytoplasm factors to inhibit virus replication. In conclusion, the present study clearly indicates that prodigiosin possesses significant antiviral activity against BmNPV.

  11. Investigation of molecular mechanisms in photodynamic action and radiobiology with nanosecond flash photolysis and pulse radiolysis

    NASA Astrophysics Data System (ADS)

    Grossweiner, L. I.

    1981-06-01

    A formula was proposed for predicting enzyme inactivation quantum yields. The predictions were in good agreement with measurements on six important enzymes at 254 nm and 280 nm. Kinetics models were developed and tested for important stages in the photosensitization of DNA to near ultraviolet radiation by furocoumarin compounds currently used for PUVA therapy (psoralen plus UV-A) of psoriasis and other human skin diseases. Experiments on photobinding of psoralen (Ps) and 8-methoxypsoralen (8-MOP) to calf thymus DNA were consistent with the assumption that equilibrium dark complexing of the furocoumarin to the DNA is a precondition for the formation of covalent monoadducts and crosslinks. Singlet oxygen generation by furocoumarins has been investigated with liposomes and human erythrocytes (rbc). Results were obtained with 3-carbethoxypsoralen (3-CPs), and experimental alternate interacts with liposome and rbc membranes in the dark.

  12. Optical Investigations of Powerful Laser Actions on Massive and Flyer Targets

    SciTech Connect

    Pisarczyk, T.; Borodziuk, S.; Kasperczuk, A.; Demchenko, N. N.; Gus'kov, S. Yu.; Jungwirth, K.; Kralikova, B.; Krousky, E.; Masek, K.; Pfeifer, M.; Rohlena, K.; Rozanov, V. B.; Skala, J.; Ullschmied, J.; Kalal, M.; Limpouch, J.; Pisarczyk, P.

    2006-01-15

    In this paper we present experimental, theoretical, and computer simulation studies of craters formation produced by high power lasers in single and double layer targets. The experimental investigation was carried out using the PALS (Prague Asterix Laser System) facility working with two different laser beam wavelengths: {lambda}1 = 1.315 {mu}m and {lambda}3 0.438 {mu}m. Two types of targets made of Al were used: single massive targets, and double targets consisting of a foil or disk (6 {mu}m thick for both cases) placed in front of the massive target part at a distance of 200-500 {mu}m. Experiments with single massive targets were performed at laser intensities in the range of 1013-1015 W/cm2 by varying the laser beam diameters on the target surface from 70 {mu}m up to 1200 {mu}m (i.e. moving the target away from the focus). The double targets were illuminated by laser energies EL = 100-500 J focused always on a diameter of 250 {mu}m. In all experiments performed the laser pulse duration was equal to 400 ps. 3-frame interferometry was employed to investigate the plasma dynamics through measurements of the electron density distribution time development as well as of the disks and foil fragments velocities. The dimensions and shapes of craters were obtained by crater replica technology and microscopy measurement. The experiments were complemented by a 2-D analytical theory and computer simulations, which helped at interpretation of the results. This way the values of laser energy absorption coefficient, ablation loading efficiency and efficiency of energy transfer, as well as two-dimensional shock wave generation at the laser-driven macroparticle impact were obtained from the measured crater parameters for both the wavelengths of laser radiation.

  13. A multi-agency investigation of Heat and human Health relationships in the state of Vermont: Towards actionable science.

    NASA Astrophysics Data System (ADS)

    Oswald, E.

    2015-12-01

    This talk focuses on an assemblage of work conducted primarily between the Vermont State Climate Office and the Vermont Department of Health for better understanding, communicating, and anticipating the impact which elevated air temperatures have, and my have in the future, on public health. This is an example in how several agencies, spanning scientific fields and levels, can all play roles in in producing important understanding and actionable consequences in the face of health risk. This talk starts with an investigation of the relationships between Vermont health statistics and daily maximum air temperature with a focus on the temperatures where the health statistics changed most rapidly with temperature changes, or "changepoints". The results of this investigation suggested that meaningful temperature changepoints exist below 90F. The local WFO considered a day as "hot" when it reached or exceeded 90F unless the day was particularly sunny and humid. Discussions with the local National Weather Service Forecast Office were productive and led to some rethinking of how they consider a "Hot" day. The changepoints information was also incorporated into a health impacts report prepared by the Vermont Department of Health for the CDC's Building Resilience Against Climate Effects, by utilizing climate indices tailored to a temperature less than 90F. This work stands as a demonstration that the co-production of knowledge can produce actionable science.

  14. Antibacterial Activity and Action Mechanism of the Essential Oil from Enteromorpha linza L. against Foodborne Pathogenic Bacteria.

    PubMed

    Patra, Jayanta Kumar; Baek, Kwang-Hyun

    2016-01-01

    Foodborne illness and disease caused by foodborne pathogenic bacteria is continuing to increase day by day and it has become an important topic of concern among various food industries. Many types of synthetic antibacterial agents have been used in food processing and food preservation; however, they are not safe and have resulted in various health-related issues. Therefore, in the present study, essential oil from an edible seaweed, Enteromorpha linza (AEO), was evaluated for its antibacterial activity against foodborne pathogens, along with the mechanism of its antibacterial action. AEO at 25 mg/disc was highly active against Bacillus cereus (12.3-12.7 mm inhibition zone) and Staphylococcus aureus (12.7-13.3 mm inhibition zone). The minimum inhibitory concentration and minimum bactericidal concentration values of AEO ranged from 12.5-25 mg/mL. Further investigation of the mechanism of action of AEO revealed its strong impairing effect on the viability of bacterial cells and membrane permeability, as indicated by a significant increase in leakage of 260 nm absorbing materials and K⁺ ions from the cell membrane and loss of high salt tolerance. Taken together, these data suggest that AEO has the potential for use as an effective antibacterial agent that functions by impairing cell membrane permeability via morphological alternations, resulting in cellular lysis and cell death. PMID:27007365

  15. Antiviral activity and mode of action of extracts from neem seed kernel against duck plague virus in vitro1.

    PubMed

    Xu, J; Song, X; Yin, Z Q; Cheng, A C; Jia, R Y; Deng, Y X; Ye, K C; Shi, C F; Lv, C; Zhang, W

    2012-11-01

    Four fractions obtained from alcohol extracts of neem (Azadirachta indica) seed kernel by column chromatography were investigated for antivirus activity against the duck plague virus (DPV) in vitro. Duck embryo fibroblasts (DEF) infected with DPV were treated with the neem seed kernel extracts, and the effect of antivirus was judged by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide colorimetric method assay and direct immunofluorescence assay. The mode of action was tested by the plaque reduction assay. The results showed that fractions 1 to 3 were inactive. The median inhibitory concentration (IC(50)) of fraction 4 was 10.9 μg/mL and inhibited the virus protein expression in the direct immunofluorescence assay. In the plaque reduction assay, fraction 4 could significantly reduce the number of plaques compared with the negative control (P < 0.01) in all modes of action. This study indicated that the fourth fraction obtained from neem seed kernel could improve the viability of infected cells, and reduce the cytopathic effects caused by DPV and the amount of the virus protein expressed in virus-infected cells. The antiviral activity works in the whole process of virus infecting the normal cells. PMID:23091135

  16. Investigations into the immunomodulatory activity of Argyreia speciosa.

    PubMed

    Gokhale, A B; Damre, A S; Saraf, M N

    2003-01-01

    The objective of the present study was to investigate the immunomodulatory activity of Argyreia speciosa on cellular and humoral immunity. Oral administration of the ethanolic extract of A. speciosa root (ASEE), at the doses of 50, 100 and 200 mg/kg in mice, dose-dependently potentiated the delayed-type hypersensitivity reaction induced both by sheep red blood cells (SRBC) and oxazolone. It significantly enhanced the production of circulating antibody titre in mice in response to SRBC. ASEE failed to show any effect on macrophage phagocytosis. Chronic administration of ASEE significantly ameliorated the total white blood cell count and also restored the myelosuppressive effects induced by cyclophosphamide. The present investigation reveals that ASEE possesses immunomodulatory activity.

  17. Probing Binding Sites and Mechanisms of Action of an IKs Activator by Computations and Experiments

    PubMed Central

    Xu, Yu; Wang, Yuhong; Zhang, Mei; Jiang, Min; Rosenhouse-Dantsker, Avia; Wassenaar, Tsjerk; Tseng, Gea-Ny

    2015-01-01

    The slow delayed rectifier (IKs) channel is composed of the KCNQ1 channel and KCNE1 auxiliary subunit, and functions to repolarize action potentials in the human heart. IKs activators may provide therapeutic efficacy for treating long QT syndromes. Here, we show that a new KCNQ1 activator, ML277, can enhance IKs amplitude in adult guinea pig and canine ventricular myocytes. We probe its binding site and mechanism of action by computational analysis based on our recently reported KCNQ1 and KCNQ1/KCNE1 3D models, followed by experimental validation. Results from a pocket analysis and docking exercise suggest that ML277 binds to a side pocket in KCNQ1 and the KCNE1-free side pocket of KCNQ1/KCNE1. Molecular-dynamics (MD) simulations based on the most favorable channel/ML277 docking configurations reveal a well-defined ML277 binding space surrounded by the S2-S3 loop and S4-S5 helix on the intracellular side, and by S4–S6 transmembrane helices on the lateral sides. A detailed analysis of MD trajectories suggests two mechanisms of ML277 action. First, ML277 restricts the conformational dynamics of the KCNQ1 pore, optimizing K+ ion coordination in the selectivity filter and increasing current amplitudes. Second, ML277 binding induces global motions in the channel, including regions critical for KCNQ1 gating transitions. We conclude that ML277 activates IKs by binding to an intersubunit space and allosterically influencing pore conductance and gating transitions. KCNE1 association protects KCNQ1 from an arrhythmogenic (constitutive current-inducing) effect of ML277, but does not preclude its current-enhancing effect. PMID:25564853

  18. Radiological survey activities: uranium mill tailings remedial action project procedures manual

    SciTech Connect

    Little, C.A.; Berven, B.A.; Carter, T.E.; Espegren, M.L.; O'Donnell, F.R.; Ramos, S.J.; Retolaza, C.D.; Rood, A.S.; Santos, F.A.; Witt, D.A.

    1986-07-01

    The US Department of Energy (DOE) was assigned the responsibility for conducting remedial action at 24 sites, which are located in one eastern and nine western states. The DOE's responsibilities are being met through its Uranium Mill Tailings Remedial Action Project Office (UMTRA-PO) in Albuquerque, New Mexico. The purpose of this Procedures Manual is to provide a standardized set of procedures that document in an auditable manner the activities performed by the Radiological Survey Activities (RASA) group in the Dosimetry and Biophysical Transport Section (DABTS) of the Health and Safety Research Division (HASRD) at the Oak Ridge National Laboratory (ORNL), in its role as the Inclusion Survey Contractor (ISC). Members of the RASA group assigned to the UMTRA Project are headquartered in the ORNL/RASA office in Grand Junction, Colorado, and report to the ORNL/RASA Project Manager. The Procedures Manual ensures that the organizational, administrative, and technical activities of the RASA/UMTRA group conform properly to those of the ISC as described in the Vicinity Properties Management and Implementation Manual and the Summary Protocol. This manual also ensures that the techniques and procedures used by the RASA/UMTRA group and contractor personnel meet the requirements of applicable governmental, scientific, and industrial standards.

  19. Unraveling the actions of AMP-activated protein kinase in metabolic diseases: Systemic to molecular insights.

    PubMed

    Weikel, Karen A; Ruderman, Neil B; Cacicedo, José M

    2016-05-01

    AMP-activated protein kinase (AMPK) plays a critical role both in sensing and regulating cellular energy state. In experimental animals, its activation has been shown to reduce the risk of obesity and diabetes-related co-morbidities such as insulin resistance, the metabolic syndrome and atherosclerotic cardiovascular disease. However, in humans, AMPK activation alone often does not completely resolve these conditions. Thus, an improved understanding of AMPK action and regulation in metabolic and other diseases is needed. Herein, we provide a brief description of the enzymatic regulation of AMPK and review its role in maintaining energy homeostasis. We then discuss tissue-specific actions of AMPK that become distorted during such conditions as obesity, type 2 diabetes and certain cancers. Finally, we explore recent findings regarding the interactions of AMPK with mammalian target of rapamycin complex 1 and the lysosome and discuss how changes in these relationships during overnutrition may lead to AMPK dysfunction. A more thorough understanding of AMPK's molecular interactions during diseases of overnutrition may provide key insights for the development of AMPK-based combinatorial treatments for metabolic disease. PMID:27085772

  20. Antioxidant activity and mechanisms of action of natural compounds isolated from lichens: a systematic review.

    PubMed

    White, Pollyanna A S; Oliveira, Rita C M; Oliveira, Aldeidia P; Serafini, Mairim R; Araújo, Adriano A S; Gelain, Daniel P; Moreira, Jose C F; Almeida, Jackson R G S; Quintans, Jullyana S S; Quintans-Junior, Lucindo J; Santos, Marcio R V

    2014-09-12

    Chronic diseases such as cancer, diabetes, neurodegenerative and cardiovascular diseases are characterized by an enhanced state of oxidative stress, which may result from the overproduction of reactive species and/or a decrease in antioxidant defenses. The search for new chemical entities with antioxidant profile is still thus an emerging field on ongoing interest. Due to the lack of reviews concerning the antioxidant activity of lichen-derived natural compounds, we performed a review of the antioxidant potential and mechanisms of action of natural compounds isolated from lichens. The search terms "lichens", "antioxidants" and "antioxidant response elements" were used to retrieve articles in LILACS, PubMed and Web of Science published until February 2014. From a total of 319 articles surveyed, 32 met the established inclusion and exclusion criteria. It was observed that the most common isolated compound studied was usnic acid, cited in 14 out of the 32 articles. The most often described antioxidant assays for the study of in vitro antioxidant activity were mainly DPPH, LPO and SOD. The most suggested mechanisms of action were scavenging of reactive species, enzymatic activation and inhibition of iNOS. Thus, compounds isolated from lichens are possible candidates for the management of oxidative stress, and may be useful in the treatment of chronic diseases.

  1. Eating tools in hand activate the brain systems for eating action: a transcranial magnetic stimulation study.

    PubMed

    Yamaguchi, Kaori; Nakamura, Kimihiro; Oga, Tatsuhide; Nakajima, Yasoichi

    2014-07-01

    There is increasing neuroimaging evidence suggesting that visually presented tools automatically activate the human sensorimotor system coding learned motor actions relevant to the visual stimuli. Such crossmodal activation may reflect a general functional property of the human motor memory and thus can be operating in other, non-limb effector organs, such as the orofacial system involved in eating. In the present study, we predicted that somatosensory signals produced by eating tools in hand covertly activate the neuromuscular systems involved in eating action. In Experiments 1 and 2, we measured motor evoked response (MEP) of the masseter muscle in normal humans to examine the possible impact of tools in hand (chopsticks and scissors) on the neuromuscular systems during the observation of food stimuli. We found that eating tools (chopsticks) enhanced the masseter MEPs more greatly than other tools (scissors) during the visual recognition of food, although this covert change in motor excitability was not detectable at the behavioral level. In Experiment 3, we further observed that chopsticks overall increased MEPs more greatly than scissors and this tool-driven increase of MEPs was greater when participants viewed food stimuli than when they viewed non-food stimuli. A joint analysis of the three experiments confirmed a significant impact of eating tools on the masseter MEPs during food recognition. Taken together, these results suggest that eating tools in hand exert a category-specific impact on the neuromuscular system for eating.

  2. Action-projection in Japanese conversation: topic particles wa, mo, and tte for triggering categorization activities

    PubMed Central

    Tanaka, Hiroko

    2015-01-01

    Conversation analytic work has revealed how anticipatory completions and preemptive actions can offer invaluable glimpses into the cognitive, contextual, grammatical, and temporal bases of projectability in turn-taking, by virtue of their potential not only as a display of participants' online prediction of roughly what it might take to complete a turn-in-progress but also to plan the next move. While the predicate-final word order and the incremental transformability of turns in Japanese generally lead to delayed projectability of turn-endings, this may be partially offset by the capacity of certain postpositional particles to trigger and propel prospective action trajectories. This article engages in a case study of the topic particle wa (and related particles mo and tte), by demonstrating how its grammatical affordances, the categorization activities, and cognitive processing it can set in motion, coupled with the immediate contextual, and temporal-productional features may coalesce to a point of critical mass, thereby enhancing the projectability of the not-yet-produced trajectory of the current turn. The discussion attempts to contribute to recent debates on ways language-specific lexicogrammatical resources are deeply interlinked with the types of opportunities that are provided for social action. PMID:26379565

  3. Learning in Activity: Exploring the Methodological Potential of Action Research in Activity Theorising of Social Practice

    ERIC Educational Resources Information Center

    Darwin, Stephen

    2011-01-01

    Cultural-historical activity theory (CHAT), founded on the seminal work of Vygotsky and evolving in the subsequent work of Leont'ev and Engestrom, continues to emerge as a robust and increasingly widely used conceptual framework for the research and analysis of the complex social mediation of human learning and development. Yet there remains…

  4. Antimicrobial Activity of Amine Oxides: Mode of Action and Structure-Activity Correlation

    PubMed Central

    Šubík, Július; Takácsová, Gizela; Pšenák, Mikuláš; Devínsky, Ferdinand

    1977-01-01

    The effect of N-alkyl derivatives of saturated heterocyclic amine oxides on the growth and metabolism of microorganisms has been studied. 4-Dodecylmorpholine-N-oxide inhibited the differentiation and growth of Bacillus cereus, of different species of filamentous fungi, and of the yeast Saccharomyces cerevisiae. For vegetative cells, the effect of 4-dodecylmorpholine-N-oxide was lethal. Cells of S. cerevisiae, after interaction with 4-dodecylmorpholine-N-oxide, released intracellular K+ and were unable to oxidize or ferment glucose. The functions of isolated yeast mitochondria were also impaired. 4-Dodecylmorpholine-N-oxide at growth-inhibiting concentrations induced rapid lysis of osmotically stabilized yeast protoplasts, with the rate of lysis a function of temperature and of amine oxide concentration. A study of the relationships between structure, antimicrobial activity, and cytolytic activity was made with a group of structurally different amine oxides involving a series of homologous 4-alkylmorpholine-N-oxides, 1-alkylpiperidine-N-oxides, 1-dodecylpyrrolidine-N-oxide, 1-dodecylperhydroasepine-N-oxide, and N,N-dimethyldodecylamine oxide. Disorganization of the membrane structure after interaction of cells with the tested amine oxides was primarily responsible for the antimicrobial activity of the amine oxides. This activity was found to be dependent on the chain length of the hydrophobic alkyl group and was only moderately influenced by other substituents of the polarized N-oxide group. PMID:409340

  5. An Action Research Inquiry into the Relationship Among Aerobic Activities, Memory, and Stress with Students Identified as Gifted

    NASA Astrophysics Data System (ADS)

    Ford, Denise Marie

    Students identified as gifted come from varying socio-economic strata and nationalities with a range of talents and temperaments comprising a diverse community. They may experience stress for a variety of reasons. Although a certain amount of stress can enhance the learning process, too much stress can impede learning, especially memory. Strategies have been offered for relieving stress, yet the benefits of physical activities as stress reducers for the gifted have frequently been overlooked. The purpose of this study was to investigate the relationship among aerobic activity, stress, and memory ability in students in an elementary school gifted program. An exceptional aspect of this research was that the students were an integral part of their own study. As co-researchers they had a vested interest in what they were doing, enhancing the significance of the experience and heightening learning. This action research project conducted in a mid-western school district with fourth and fifth grade students examined the impact of aerobic movement on physical indicators of stress and memory. The study lasted twelve weeks with data collected on physical indicators of stress, memory test scores, parent observations, interviews with students, a parent focus group session, observational data, student comments, and investigator/teacher journal. By infusing regular exercise into curricula, stress levels in students identified as gifted were examined. Students' scores on declarative memory tasks conducted with and without an accompanying aerobic activity were documented. Students learned of the delicate relationship between stress and memory as they studied the physiology of the brain. Twenty-four hour retention rates of declarative memory items were higher when a 20-minute aerobic activity intervention preceded the memory activity. Perceived stress levels were lowered for 14 of the 16 co-researchers. Students indicated a positive attitude toward physical activity and its

  6. Anti-inflammatory action of legume isoflavonoid sophoricoside through inhibition on cyclooxygenase-2 activity.

    PubMed

    Kim, Byung Hak; Chung, Eun Yong; Min, Bo-Kyung; Lee, Seung Ho; Kim, Mi-Kyeong; Min, Kyung Rak; Kim, Youngsoo

    2003-05-01

    Soy is a main source of isoflavonoids which are of high dietary intake for the oriental population. In this study, the anti-inflammatory action of sophoricoside, an isoflavone glycoside isolated from immature fruits of Sophora japonica L. (Leguminosae), has been demonstrated. When administered orally at > 100 mg/kg or injected intravenously at > 10 mg/kg, sophoricoside showed significant reduction of carrageenin-induced paw edema in mice. Sophoricoside has been identified as a selective inhibitor of cyclooxygenase (COX)-2 activity with an IC50 value of 3.3 microM. PMID:12802736

  7. Evaluation of the legal consequences of action affects neural activity and emotional experience during the resolution of moral dilemmas.

    PubMed

    Pletti, Carolina; Sarlo, Michela; Palomba, Daniela; Rumiati, Rino; Lotto, Lorella

    2015-03-01

    In any modern society killing is regarded as a severe violation of the legal codes that is subjected to penal judgment. Therefore, it is likely that people take legal consequences into account when deciding about the hypothetical killing of one person in classic moral dilemmas, with legal concerns contributing to decision-making. In particular, by differing for the degree of intentionality and emotional salience, Footbridge- and Trolley-type dilemmas might promote differential assignment of blame and punishment while implicating the same severity of harm. The present study was aimed at comparing the neural activity, subjective emotional reactions, and behavioral choices in two groups of participants who either took (Legal group) or did not take (No Legal group) legal consequences into account when deciding on Footbridge-type and Trolley-type moral dilemmas. Stimulus- and response-locked ERPs were measured to investigate the neural activity underlying two separate phases of the decision process. No difference in behavioral choices was found between groups. However, the No Legal group reported greater overall emotional impact, associated with lower preparation for action, suggesting greater conflict between alternative motor responses representing the different decision choices. In contrast, the Legal group showed an overall dampened affective experience during decision-making associated with greater overall action readiness and intention to act, reflecting lower conflict in responding. On these bases, we suggest that in moral dilemmas legal consequences of actions provide a sort of reference point on which people can rely to support a decision, independent of dilemma type. PMID:25638294

  8. Investigations on the sporicidal and fungicidal activity of disinfectants.

    PubMed

    Lensing, H H; Oei, H L

    1985-12-01

    The sporicidal and fungicidal activity of disinfectants was studied in a suspension test. Glutaraldehyde 4%, sodium-dichloroisocyanurate-dihydrate (2400 ppm active chlorine) and peracetic acid 0.25% demonstrated after 30 min of exposure at 20 degrees C in the presence of 4% horse serum a clear activity against spores of Bacillus cereus. Under the same conditions formaldehyde 4% and glutaraldehyde 2% were also found to be sporicidal, but only after a longer time of exposure. Spores of Bacillus anthracis and B. cereus appeared to be comparably resistant against the investigated disinfectants, whereas conidiospores of Aspergillus fumigatus and Aspergillus niger were less resistant. Of the micro-organisms tested Candida albicans proved to be slightest resistant, while spores of Bacillus subtilis were found the most resistant. PMID:3938146

  9. Investigating Deformylase and Deacylase Activity of Mammalian and Bacterial Sirtuins.

    PubMed

    Seidel, Julian; Klockenbusch, Cordula; Schwarzer, Dirk

    2016-03-01

    Lysine acylation constitutes a major group of post-translational modifications of proteins, and is found in the proteomes of organisms from all kingdoms of life. Sirtuins are considered the main erasers of these modification marks, and thus contribute to acylation-dependent regulation of enzyme activity, and potentially of protein quality control. We have established a substrate scaffold to enable the analysis of sirtuin activity with a broad range of acyl-lysine modifications, including hydrophobic fatty acids. Characterization of the deacylase activity of the bacterial SrtN, which is encoded by the yhdZ gene of Bacillus subtilis, showed that this enzyme is capable of removing a broad range of acyl groups. These investigations further showed that SrtN and human SIRT1 are efficient lysine-deformylases, thereby providing a first clue as to how this nonenzymatic modification might be removed from affected proteins.

  10. Corrective Action Investigation Plan for Corrective Action Unit 527: Horn Silver Mine, Nevada Test Site, Nevada: Revision 1 (Including Records of Technical Change No.1, 2, 3, and 4)

    SciTech Connect

    U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office

    2002-12-06

    This Corrective Action Investigation Plan contains the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Operations Office's approach to collect the data necessary to evaluate corrective action alternatives appropriate for the closure of Corrective Action Unit (CAU) 527, Horn Silver Mine, Nevada Test Site, Nevada, under the Federal Facility Agreement and Consent Order. Corrective Action Unit 527 consists of one Corrective Action Site (CAS): 26-20-01, Contaminated Waste Dump No.1. The site is located in an abandoned mine site in Area 26 (which is the most arid part of the NTS) approximately 65 miles northwest of Las Vegas. Historical documents may refer to this site as CAU 168, CWD-1, the Wingfield mine (or shaft), and the Wahmonie mine (or shaft). Historical documentation indicates that between 1959 and the 1970s, nonliquid classified material and unclassified waste was placed in the Horn Silver Mine's shaft. Some of the waste is known to be radioactive. Documentation indicates that the waste is present from 150 feet to the bottom of the mine (500 ft below ground surface). This CAU is being investigated because hazardous constituents migrating from materials and/or wastes disposed of in the Horn Silver Mine may pose a threat to human health and the environment as well as to assess the potential impacts associated with any potential releases from the waste. The results of this field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  11. An Evaluation of the My ParticipACTION Campaign to Increase Self-Efficacy for Being More Physically Active.

    PubMed

    Craig, Cora Lynn; Bauman, Adrian; Latimer-Cheung, Amy; Rhodes, Ryan E; Faulkner, Guy; Berry, Tanya R; Tremblay, Mark S; Spence, John C

    2015-01-01

    The objective of the My ParticipACTION campaign was to inspire Canadian adults to increase their physical activity through messaging that was relevant, engaging, and designed to build self-efficacy to be more active. This research examined the communication effects of the campaign according to the a priori Hierarchy of Effects Model (saliency → cognitive engagement → self-efficacy to become more active → trial behavior) and investigated how these effects related to overall self-efficacy for physical activity, intention to be active, and current activity level. Participants (N = 1,110) were recruited from an existing panel of Canadian adults 18 years and older and completed a short online questionnaire about the potential communication effects. Logistic regression models were constructed to test the communication effects adjusting for age, gender, and education. The relations were consistent with those hypothesized in the model. In addition, some earlier outcomes in the sequence of effects were associated with other outcomes further down the progression. When intention to be active was included, the initial relation between ad-specific self-efficacy and current physical activity disappeared. This analysis suggested that the campaign was successful in increasing self-efficacy to be more active and that using the Hierarchy of Effects Model was useful in guiding the design of campaign messages and assessing communication effects. Given the limited amount of theoretical testing of the Hierarchy of Effects Model, future research employing longitudinal designs is required to further confirm the communication effects of such an intervention and further test the model. PMID:26151315

  12. Investigation of compressive membrane action in ultra high performance concrete slab strips

    NASA Astrophysics Data System (ADS)

    Foust, Bradley Wade

    Reinforced concrete slabs are found in very common structural systems in both civilian and military applications. The boundary conditions that support the slab play an important role in the response to a particular load. Specifically, the amount of lateral and rotational restraint dictates how a slab responds to a particular load. Compressive membrane (i.e., in-plane) forces are present in slabs when the boundaries are sufficiently stiff, therefore restricting the slab from both lateral translations and rotations. Advancements have been made to account for the additional capacity due to compressive membrane forces in conventional strength concrete. In today's world, concrete performance is improving because of increasing compressive strengths and additional ductility present in concrete members. As a result of this current improvement, there is an urgent need to investigate compressive membrane theory in ultra-high-performance concrete (UHPC) slabs to better understand their behavior. Existing compressive membrane theory should be revisited to determine if current theory is applicable, or if it is not, what modifications should be made. This study will provide insight into the validity of existing theory that is currently used to predict the ultimate capacity in conventional-strength concrete slabs and attempt to modify the existing equations to account for high-strength concrete materials. A matrix of 14 normal-strength concrete (NSC) and 13 UHPC slabs was tested both statically and dynamically to better understand the behavior of each material set and the effects that boundary conditions have on slab response. The results from these experiments were then compared to response calculations made from existing theory as well as finite element analyses. Valuable data sets on rigidly restrained UHPC slab response were obtained through an experimental research program. The experiments helped to validate the associated numerical analysis that was performed. It was

  13. Action of ubenimex on aminopeptidase activities in spleen cells and peritoneal macrophages from mice.

    PubMed

    Kuramochi, H; Motegi, A; Iwabuchi, M; Takahashi, K; Horinishi, H; Umezawa, H

    1987-11-01

    The action of ubenimex on aminopeptidase (APase) activity was studied in intact spleen cells and peritoneal macrophages from mice. Ubenimex strongly inhibited hydrolyzing activities against arginine-beta-naphtylamide (Arg-NA), Lys-NA and Pro-NA in both cells. Inhibition of hydrolysis of Leu-NA, Met-NA and Ala-NA was relatively small or not observed. When both cells were incubated in HANKS' solution, hydrolyzing activities against Arg-NA, Lys-NA and Pro-NA were released to the medium, while Leu-NA and Met-NA-hydrolyzing activities were mostly bound. In addition, the Leu-NA-hydrolyzing activity in the spleen cells was kinetically studied. The Arg-NA and Leu-NA-hydrolyzing activities in four fractions prepared from the homogenate of spleen cells were also studied kinetically. From these studies it was suggested that ubenimex inhibits aminopeptidase B and a Pro-NA-hydrolyzing enzyme more effectively than Leu-APase in intact spleen cells and peritoneal macrophages from mice.

  14. Alignment effects in beer mugs: Automatic action activation or response competition?

    PubMed

    Roest, Sander A; Pecher, Diane; Naeije, Lilian; Zeelenberg, René

    2016-08-01

    Responses to objects with a graspable handle are faster when the response hand and handle orientation are aligned (e.g., a key press with the right hand is required and the object handle is oriented to the right) than when they are not aligned. This effect could be explained by automatic activation of specific motor programs when an object is viewed. Alternatively, the effect could be explained by competition at the response level. Participants performed a reach-and-grasp or reach-and-button-press action with their left or right hand in response to the color of a beer mug. The alignment effect did not vary as a function of the type of action. In addition, the alignment effect disappeared in a go/no-go version of the task. The same results were obtained when participants made upright/inverted decisions, so that object shape was task-relevant. Our results indicate that alignment effects are not due to automatic motor activation of the left or right limb.

  15. Alignment effects in beer mugs: Automatic action activation or response competition?

    PubMed

    Roest, Sander A; Pecher, Diane; Naeije, Lilian; Zeelenberg, René

    2016-08-01

    Responses to objects with a graspable handle are faster when the response hand and handle orientation are aligned (e.g., a key press with the right hand is required and the object handle is oriented to the right) than when they are not aligned. This effect could be explained by automatic activation of specific motor programs when an object is viewed. Alternatively, the effect could be explained by competition at the response level. Participants performed a reach-and-grasp or reach-and-button-press action with their left or right hand in response to the color of a beer mug. The alignment effect did not vary as a function of the type of action. In addition, the alignment effect disappeared in a go/no-go version of the task. The same results were obtained when participants made upright/inverted decisions, so that object shape was task-relevant. Our results indicate that alignment effects are not due to automatic motor activation of the left or right limb. PMID:27184058

  16. Drilling to investigate processes in active tectonics and magmatism

    NASA Astrophysics Data System (ADS)

    Shervais, J.; Evans, J.; Toy, V.; Kirkpatrick, J.; Clarke, A.; Eichelberger, J.

    2014-12-01

    Coordinated drilling efforts are an important method to investigate active tectonics and magmatic processes related to faults and volcanoes. The US National Science Foundation (NSF) recently sponsored a series of workshops to define the nature of future continental drilling efforts. As part of this series, we convened a workshop to explore how continental scientific drilling can be used to better understand active tectonic and magmatic processes. The workshop, held in Park City, Utah, in May 2013, was attended by 41 investigators from seven countries. Participants were asked to define compelling scientific justifications for examining problems that can be addressed by coordinated programs of continental scientific drilling and related site investigations. They were also asked to evaluate a wide range of proposed drilling projects, based on white papers submitted prior to the workshop. Participants working on faults and fault zone processes highlighted two overarching topics with exciting potential for future scientific drilling research: (1) the seismic cycle and (2) the mechanics and architecture of fault zones. Recommended projects target fundamental mechanical processes and controls on faulting, and range from induced earthquakes and earthquake initiation to investigations of detachment fault mechanics and fluid flow in fault zones. Participants working on active volcanism identified five themes: the volcano eruption cycle; eruption sustainability, near-field stresses, and system recovery; eruption hazards; verification of geophysical models; and interactions with other Earth systems. Recommended projects address problems that are transferrable to other volcanic systems, such as improved methods for identifying eruption history and constraining the rheological structure of shallow caldera regions. Participants working on chemical geodynamics identified four major themes: large igneous provinces (LIPs), ocean islands, continental hotspot tracks and rifts, and

  17. Coordinated Action of Fast and Slow Reserves for Optimal Sequential and Dynamic Emergency Reserve Activation

    NASA Astrophysics Data System (ADS)

    Salkuti, Surender Reddy; Bijwe, P. R.; Abhyankar, A. R.

    2016-04-01

    This paper proposes an optimal dynamic reserve activation plan after the occurrence of an emergency situation (generator/transmission line outage, load increase or both). An optimal plan is developed to handle the emergency situation, using coordinated action of fast and slow reserves, for secure operation with minimum overall cost. This paper considers the reserves supplied by generators (spinning reserves) and loads (demand-side reserves). The optimal backing down of costly/fast reserves and bringing up of slow reserves in each sub-interval in an integrated manner is proposed. The simulation studies are performed on IEEE 30, 57 and 300 bus test systems to demonstrate the advantage of proposed integrated/dynamic reserve activation plan over the conventional/sequential approach.

  18. Describing the Mechanism of Antimicrobial Peptide Action with the Interfacial Activity Model

    PubMed Central

    Wimley, William C.

    2010-01-01

    Antimicrobial peptides (AMPs) have been studied for three decades, and yet a molecular understanding of their mechanism of action is still lacking. Here we summarize current knowledge for both synthetic vesicle experiments and microbe experiments, with a focus on comparisons between the two. Microbial experiments are done at peptide:lipid ratios that are at least 4 orders of magnitude higher than vesicle-based experiments. To close the gap between the two concentration regimes, we propose an “interfacial activity model”, which is based on an experimentally testable molecular image of AMP-membrane interactions. The interfacial activity model may be useful in driving engineering and design of novel AMPs. PMID:20698568

  19. Quaternized Chitosan as an Antimicrobial Agent: Antimicrobial Activity, Mechanism of Action and Biomedical Applications in Orthopedics

    PubMed Central

    Tan, Honglue; Ma, Rui; Lin, Chucheng; Liu, Ziwei; Tang, Tingting

    2013-01-01

    Chitosan (CS) is a linear polysaccharide with good biodegradability, biocompatibility and antimicrobial activity, which makes it potentially useful for biomedical applications, including an antimicrobial agent either alone or blended with other polymers. However, the poor solubility of CS in most solvents at neutral or high pH substantially limits its use. Quaternary ammonium CS, which was prepared by introducing a quaternary ammonium group on a dissociative hydroxyl group or amino group of the CS, exhibited improved water solubility and stronger antibacterial activity relative to CS over an entire range of pH values; thus, this quaternary modification increases the potential biomedical applications of CS in the field of anti-infection. This review discusses the current findings on the antimicrobial properties of quaternized CS synthesized using different methods and the mechanisms of its antimicrobial actions. The potential antimicrobial applications in the orthopedic field and perspectives regarding future studies in this field are also considered. PMID:23325051

  20. Role of habitual physical activity in modulating vascular actions of insulin

    PubMed Central

    Padilla, Jaume; Olver, T. Dylan; Thyfault, John P.; Fadel, Paul J.

    2016-01-01

    Type 2 diabetes and cardiovascular disease commonly coexist. Current evidence suggests that impaired insulin signalling in the vasculature may be a common link between metabolic and cardiovascular diseases, including glycaemic dysregulation and atherosclerosis. Herein, we highlight the importance of the actions of insulin on the vasculature for glycaemic control and arterial health. In addition, we summarize and discuss findings from our group and others demonstrating that increased physical activity may be an effective approach to enhancing vascular insulin sensitivity. Furthermore, in light of the existing literature, we formulate the hypothesis that increased shear stress may be a prime mechanism through which habitual physical activity improves insulin signalling in the vasculature. Ultimately, we propose that targeting vascular insulin resistance may represent a viable strategy for improving glycaemic control and reducing cardiovascular risk in patients with type 2 diabetes. PMID:26130183

  1. Call to Action on Making Physical Activity Assessment and Prescription a Medical Standard of Care.

    PubMed

    Sallis, Robert E; Matuszak, Jason M; Baggish, Aaron L; Franklin, Barry A; Chodzko-Zajko, Wojtek; Fletcher, Barbara J; Gregory, Andrew; Joy, Elizabeth; Matheson, Gordon; McBride, Patrick; Puffer, James C; Trilk, Jennifer; Williams, Janet

    2016-01-01

    The U.S. population is plagued by physical inactivity, lack of cardiorespiratory fitness, and sedentary lifestyles, all of which are strongly associated with the emerging epidemic of chronic disease. The time is right to incorporate physical activity assessment and promotion into health care in a manner that engages clinicians and patients. In April 2015, the American College of Sports Medicine and Kaiser Permanente convened a joint consensus meeting of subject matter experts from stakeholder organizations to discuss the development and implementation of a physical activity vital sign (PAVS) to be obtained and recorded at every medical visit for every patient. This statement represents a summary of the discussion, recommendations, and next steps developed during the consensus meeting. Foremost, it is a "call to action" for current and future clinicians and the health care community to implement a PAVS in daily practice with every patient. PMID:27172086

  2. Investigating the physiology of brain activation with MRI

    NASA Astrophysics Data System (ADS)

    Buxton, Richard B.; Uludag, Kamil; Dubowitz, David J.

    2004-04-01

    Functional magnetic resonance imaging (fMRI) has become a powerful tool for investigating the working human brain based on the blood oxygenation level dependent (BOLD) effect on the MR signal. However, despite the widespread use of fMRI techniques for mapping brain activation, the basic physiological mechanisms underlying the observed signal changes are still poorly understood. Arterial spin labeling (ASL) techniques, which measure cerebral blood flow (CBF) and the BOLD effect simultaneously, provide a useful tool for investigating these physiological questions. In this paper, recent results of studies manipulating the baseline CBF both pharmacologically and physiologically will be discussed. These data are consistent with a feed-forward mechanism of neurovascular coupling, and suggest that the CBF change itself may be a more robust reflection of neural activity changes than the BOLD effect. Consistent with these data, a new thermodynamic hypothesis is proposed for the physiological function of CBF regulation: maintenance of the [O2]/[CO2] concentration ratio at the mitochondria in order to preserve the free energy available from oxidative metabolism. A kinetic model based on this hypothesis provides a reasonable quantitative description of the CBF changes associated with neural activity and altered blood gases (CO2 and O2).

  3. Effects of the imidazobenzodiazepine R015-4513 on the stimulant and depressant actions of ethanol on spontaneous locomotor activity

    SciTech Connect

    Becker, H.C.

    1988-01-01

    The purpose of this study was to investigate the effects of the imidazobenzodiazepine R015-4513, a partial inverse agonist at benzodiazepine (BDZ) receptors, on the stimulant and depressant actions of ethanol in mice. For comparative purposes, another BDZ inverse agonist, FG-7142, was examined as well. Neither R015-4513 nor FG-7142 influenced the low-dose excitatory effects of ethanol on spontaneous locomotor activity. However, both R015-4513 and FG-7142 significantly antagonized the depressant effects of ethanol, and this antagonism was completely reversed by pretreatment with the BDZ receptor antagonist, R015-1788. These data suggest that R015-4513 is capable of antagonizing only some of the behavioral effects of ethanol, and in particular, those responses to ethanol that are mediated by modulation of the GABA/BDZ-chloride channel receptor complex.

  4. Optical Tools to Investigate Cellular Activity in the Intestinal Wall

    PubMed Central

    Boesmans, Werend; Hao, Marlene M; Berghe, Pieter Vanden

    2015-01-01

    Live imaging has become an essential tool to investigate the coordinated activity and output of cellular networks. Within the last decade, 2 Nobel prizes have been awarded to recognize innovations in the field of imaging: one for the discovery, use, and optimization of the green fluorescent protein (2008) and the second for the development of super-resolved fluorescence microscopy (2014). New advances in both optogenetics and microscopy now enable researchers to record and manipulate activity from specific populations of cells with better contrast and resolution, at higher speeds, and deeper into live tissues. In this review, we will discuss some of the recent developments in microscope technology and in the synthesis of fluorescent probes, both synthetic and genetically encoded. We focus on how live imaging of cellular physiology has progressed our understanding of the control of gastrointestinal motility, and we discuss the hurdles to overcome in order to apply the novel tools in the field of neurogastroenterology and motility. PMID:26130630

  5. AN ULTRAVIOLET INVESTIGATION OF ACTIVITY ON EXOPLANET HOST STARS

    SciTech Connect

    Shkolnik, Evgenya L.

    2013-03-20

    Using the far-UV (FUV) and near-UV (NUV) photometry from the NASA Galaxy Evolution Explorer (GALEX), we searched for evidence of increased stellar activity due to tidal and/or magnetic star-planet interactions (SPI) in the 272 known FGK planetary hosts observed by GALEX. With the increased sensitivity of GALEX, we are able probe systems with lower activity levels and at larger distances than what has been done to date with X-ray satellites. We compared samples of stars with close-in planets (a < 0.1 AU) to those with far-out planets (a > 0.5 AU) and looked for correlations of excess activity with other system parameters. This statistical investigation found no clear correlations with a, M{sub p} , or M{sub p} /a, in contrast to some X-ray and Ca II studies. However, there is tentative evidence (at a level of 1.8{sigma}) that stars with radial-velocity-(RV)-detected close-in planets are more FUV-active than stars with far-out planets, in agreement with several published X-ray and Ca II results. The case is strengthened to a level of significance to 2.3{sigma} when transit-detected close-in planets are included. This is most likely because the RV-selected sample of stars is significantly less active than the field population of comparable stars, while the transit-selected sample is similarly active. Given the factor of 2-3 scatter in fractional FUV luminosity for a given stellar effective temperature, it is necessary to conduct a time-resolved study of the planet hosts in order to better characterize their UV variability and generate a firmer statistical result.

  6. Investigation of some biologic activities of Swertia longifolia Boiss.

    PubMed

    Hajimehdipoor, H; Esmaeili, S; Shekarchi, M; Emrarian, T; Naghibi, F

    2013-10-01

    Swertia species are widespread in Eastern and Southern Asian countries and used in traditional medicine as anti-pyretic, analgesic, gastro and liver tonic. Among different species, only Swertia longifolia grows in Iran. In this investigation, antioxidant, cytotoxic and acetylcholinesterase inhibitory activities of S. longifolia have been studied. Aerial parts and roots of the plant were collected, dried and extracted with methanol 80% (total extract). Different extracts of the plant were obtained using hexane, chloroform, ethyl acetate, methanol, methanol:water (1:1) and water, respectively. Cytotoxic activity was determined by MTT assay on MDBK, HepG2, MCF7, HT29 and A549 cell lines. Antioxidant activity was measured by 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radicals and acetylcholinesterase inhibitory (AChEI) effect was evaluated based on Ellman's method in 96-well microplates.The results showed no cytotoxicity of the plant extracts on MDBK, HepG2, MCF7, HT29 and A549 cell lines up to 100 μg/ml. All samples showed radical scavenging activity but methanol extract of aerial parts and ethyl acetate extract of the roots showed the highest effects.Total extract of the roots showed higher AChEI activity than the aerial parts. Among different extracts, chloroform and ethyl acetate extracts of the roots and chloroform and methanol:water extracts of the aerial parts were more potent in AChEI assay. It is concluded that aerial parts and roots of the plant are rich in antioxidant agents with no cytotoxicity on selected cell lines up to 100 μg/ml. Moreover, since antioxidant and AChEI activity of compounds play an important role in the treatment of Alzheimer's disorder, this plant might be a potential candidate for isolation of antioxidant and AChEI compounds which could be used as supportive treatment of Alzheimer's disease. PMID:24082894

  7. LDEF meteoroid and debris special investigation group investigations and activities at the Johnson Space Center

    NASA Technical Reports Server (NTRS)

    See, Thomas H.; Warren, Jack L.; Zolensky, Michael E.; Sapp, Clyde A.; Bernhard, Ronald P.; Dardano, Claire B.

    1995-01-01

    Since the return of the Long Duration Exposure Facility (LDEF) in January, 1990, members of the Meteoroid and Debris Special Investigation Group (M&D SIG) at the Johnson Space Center (JSC) in Houston, Texas have been examining LDEF hardware in an effort to expand the knowledge base regarding the low-Earth orbit (LEO) particulate environment. In addition to the various investigative activities, JSC is also the location of the general Meteoroid & Debris database. This publicly accessible database contains information obtained from the various M&D SIG investigations, as well as limited data obtained by individual LDEF Principal Investigators. LDEF exposed approximately 130 m(exp 2) of surface area to the LEO particulate environment, approximately 15.4 m(exp 2) of which was occupied by structural frame components (i.e., longerons and intercoastals) of the spacecraft. The data reported here was obtained as a result of detailed scans of LDEF intercoastals, 68 of which reside at JSC. The limited amount of data presently available on the A0178 thermal control blankets was reported last year and will not be reiterated here. The data presented here are limited to measurements of crater diameters and their frequency of occurrence (i.e., flux).

  8. Activated Stat5 trafficking Via Endothelial Cell-derived Extracellular Vesicles Controls IL-3 Pro-angiogenic Paracrine Action.

    PubMed

    Lombardo, Giusy; Dentelli, Patrizia; Togliatto, Gabriele; Rosso, Arturo; Gili, Maddalena; Gallo, Sara; Deregibus, Maria Chiara; Camussi, Giovanni; Brizzi, Maria Felice

    2016-05-09

    Soluble factors and cell-derived extracellular vesicles (EVs) control vascular cell fate during inflammation. The present study investigates the impact of Interleukin 3 (IL-3) on EV release by endothelial cells (ECs), the mechanisms involved in EV release and paracrine actions. We found that IL-3 increases EV release, which is prevented by IL-3Ralpha blockade. EVs released upon IL-3 stimulation were able to induce pro-angiogenic signals as shown by chromatin immunoprecipitation (ChIP) assay performed on the promoter region of cyclin D1 and tridimensional tube-like structure formation. We herein demonstrate that these effects rely on the transfer of miR-126-3p, pre-miR-126 and, more importantly, of activated signal transduction and activator of transcription 5 (pSTAT5) from IL-3-EV cargo into recipient ECs. We show, using the dominant negative form (ΔN)STAT5 and an activated STAT5 (1*6STAT5) constructs, that STAT5 drives IL-3-mediated EV release, miR-126-3p and pSTAT5 content. Finally, using EVs recovered from ΔNSTAT5 expressing ECs, we provide evidence that miR-126-3p and pSTAT5 trafficking is relevant for IL-3-mediated paracrine pro-angiogenic signals. These results indicate that IL-3 regulates EC-EV release, cargo and IL-3 angiogenic paracrine action via STAT5. Moreover, these results provide evidence that EC-derived IL-3-EVs can serve as pro-angiogenic clinical delivery wound healing devices.

  9. Activated Stat5 trafficking Via Endothelial Cell-derived Extracellular Vesicles Controls IL-3 Pro-angiogenic Paracrine Action

    PubMed Central

    Lombardo, Giusy; Dentelli, Patrizia; Togliatto, Gabriele; Rosso, Arturo; Gili, Maddalena; Gallo, Sara; Deregibus, Maria Chiara; Camussi, Giovanni; Brizzi, Maria Felice

    2016-01-01

    Soluble factors and cell-derived extracellular vesicles (EVs) control vascular cell fate during inflammation. The present study investigates the impact of Interleukin 3 (IL-3) on EV release by endothelial cells (ECs), the mechanisms involved in EV release and paracrine actions. We found that IL-3 increases EV release, which is prevented by IL-3Ralpha blockade. EVs released upon IL-3 stimulation were able to induce pro-angiogenic signals as shown by chromatin immunoprecipitation (ChIP) assay performed on the promoter region of cyclin D1 and tridimensional tube-like structure formation. We herein demonstrate that these effects rely on the transfer of miR-126-3p, pre-miR-126 and, more importantly, of activated signal transduction and activator of transcription 5 (pSTAT5) from IL-3-EV cargo into recipient ECs. We show, using the dominant negative form (ΔN)STAT5 and an activated STAT5 (1*6STAT5) constructs, that STAT5 drives IL-3-mediated EV release, miR-126-3p and pSTAT5 content. Finally, using EVs recovered from ΔNSTAT5 expressing ECs, we provide evidence that miR-126-3p and pSTAT5 trafficking is relevant for IL-3-mediated paracrine pro-angiogenic signals. These results indicate that IL-3 regulates EC-EV release, cargo and IL-3 angiogenic paracrine action via STAT5. Moreover, these results provide evidence that EC-derived IL-3-EVs can serve as pro-angiogenic clinical delivery wound healing devices. PMID:27157262

  10. Defining the epigenetic actions of growth hormone: acute chromatin changes accompany GH-activated gene transcription.

    PubMed

    Chia, Dennis J; Rotwein, Peter

    2010-10-01

    Many of the long-term physiological effects of GH require hormone-mediated changes in gene expression. The transcription factor signal transducer and activator of transcription 5b (Stat5b) plays a critical role in the actions of GH on growth and metabolism by regulating a large number of GH-dependent genes by incompletely understood mechanisms. Here we have assessed the impact of GH-initiated and Stat5b-mediated signaling on the chromatin landscape of hormone-regulated genes in the liver of pituitary-deficient young adult male rats. In the absence of GH there was minimal ongoing transcription at the Socs2, Cish, Igfals, and Spi 2.1 promoters, minimal occupancy of Stat5b at proximal promoter sites, and relatively closed chromatin, as evidenced by low levels of core histone acetylation. In contrast, transcriptionally silent Igf1 promoter 1 appeared poised to be activated, based on binding of coactivators p300 and Med1/Trap220, high levels of histone acetylation, and the presence of RNA polymerase II. GH treatment led to a 8- to 20-fold rise in transcriptional activity of all five genes within 30-60 min and was accompanied by binding of Stat5b to the proximal Socs2, Cish, Igfals, and Spi 2.1 promoters and to seven distal Igf1 Stat5b elements, by enhanced histone acetylation at all five promoters, by recruitment of RNA polymerase II to the Socs2, Cish, Igfals, and Spi 2.1 promoters, and by loss of the transcriptional repressor Bcl6 from Socs2, Cish, and Igfals Stat5b sites, but not from two Igf1 Stat5b domains. We conclude that GH actions induce rapid and dramatic changes in hepatic chromatin at target promoters and propose that the chromatin signature of Igf1 differs from other GH-and Stat5b-dependent genes. PMID:20702579

  11. NADPH oxidase-mediated Rac1 GTP activity is necessary for nongenomic actions of the mineralocorticoid receptor in the CA1 region of the rat hippocampus.

    PubMed

    Kawakami-Mori, Fumiko; Shimosawa, Tatsuo; Mu, Shengyu; Wang, Hong; Ogura, Sayoko; Yatomi, Yutaka; Fujita, Toshiro

    2012-02-15

    Mineralocorticoid receptors (MRs) in the central nervous system play important roles in spatial memory, fear memory, salt sensitivity, and hypertension. Corticosterone binds to MRs to induce presynaptic vesicle release and postsynaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor aggregation, which are necessary for induction of long-term potentiation under psychological stress. On the other hand, cognitive dysfunction is an important problem clinically in patients with hypertension, diabetes, and cerebral infarction, and all of these conditions are associated with an increase in reactive oxygen species (ROS) generation. Oxidative stress has been shown to modify the genomic actions of MRs in the peripheral organs; however, there have been no reports until now about the relation between the nongenomic actions of MRs and ROS in the central nervous system. In this study, we investigated the relationship between ROS and the nongenomic actions of MR. We examined the nongenomic actions of MR by measuring the slope of the field excitatory postsynaptic potentials and found that ROS induced an additive increase of these potentials, which was accompanied by Rac1 GTP activation and ERK1/2 phosphorylation. An NADPH oxidase inhibitor, apocynin, blocked the nongenomic actions of MRs. A Rac1 inhibitor, NSC23766, was also found to block synaptic enhancement and ERK1/2 phosphorylation induced by NADPH and corticosterone. We concluded that NADPH oxidase activity and Rac1 GTP activity are indispensable for the nongenomic actions of MRs and that Rac1 GTP activation induces ERK1/2 phosphorylation in the brain.

  12. Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiology and Exerts Neuroprotective Actions In Vitro

    PubMed Central

    Martins, João; Elvas, Filipe; Brudzewsky, Dan; Martins, Tânia; Kolomiets, Bogdan; Tralhão, Pedro; Gøtzsche, Casper R.; Cavadas, Cláudia; Castelo-Branco, Miguel; Woldbye, David P. D.; Picaud, Serge; Santiago, Ana R.

    2015-01-01

    Neuropeptide Y (NPY) is expressed in mammalian retina but the location and potential modulatory effects of NPY receptor activation remain largely unknown. Retinal ganglion cell (RGC) death is a hallmark of several retinal degenerative diseases, particularly glaucoma. Using purified RGCs and ex vivo rat retinal preparations, we have measured RGC intracellular free calcium concentration ([Ca2+]i) and RGC spiking activity, respectively. We found that NPY attenuated the increase in the [Ca2+]i triggered by glutamate mainly via Y1 receptor activation. Moreover, (Leu31, Pro34)−NPY, a Y1/Y5 receptor agonist, increased the initial burst response of OFF-type RGCs, although no effect was observed on RGC spontaneous spiking activity. The Y1 receptor activation was also able to directly modulate RGC responses by attenuating the NMDA-induced increase in RGC spiking activity. These results suggest that Y1 receptor activation, at the level of inner or outer plexiform layers, leads to modulation of RGC receptive field properties. Using in vitro cultures of rat retinal explants exposed to NMDA, we found that NPY pretreatment prevented NMDA-induced cell death. However, in an animal model of retinal ischemia-reperfusion injury, pretreatment with NPY or (Leu31, Pro34)−NPY was not able to prevent apoptosis or rescue RGCs. In conclusion, we found modulatory effects of NPY application that for the first time were detected at the level of RGCs. However, further studies are needed to evaluate whether NPY neuroprotective actions detected in retinal explants can be translated into animal models of retinal degenerative diseases. PMID:26311075

  13. Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiology and Exerts Neuroprotective Actions In Vitro.

    PubMed

    Martins, João; Elvas, Filipe; Brudzewsky, Dan; Martins, Tânia; Kolomiets, Bogdan; Tralhão, Pedro; Gøtzsche, Casper R; Cavadas, Cláudia; Castelo-Branco, Miguel; Woldbye, David P D; Picaud, Serge; Santiago, Ana R; Ambrósio, António F

    2015-01-01

    Neuropeptide Y (NPY) is expressed in mammalian retina but the location and potential modulatory effects of NPY receptor activation remain largely unknown. Retinal ganglion cell (RGC) death is a hallmark of several retinal degenerative diseases, particularly glaucoma. Using purified RGCs and ex vivo rat retinal preparations, we have measured RGC intracellular free calcium concentration ([Ca2+]i) and RGC spiking activity, respectively. We found that NPY attenuated the increase in the [Ca2+]i triggered by glutamate mainly via Y1 receptor activation. Moreover, (Leu31, Pro34)-NPY, a Y1/Y5 receptor agonist, increased the initial burst response of OFF-type RGCs, although no effect was observed on RGC spontaneous spiking activity. The Y1 receptor activation was also able to directly modulate RGC responses by attenuating the NMDA-induced increase in RGC spiking activity. These results suggest that Y1 receptor activation, at the level of inner or outer plexiform layers, leads to modulation of RGC receptive field properties. Using in vitro cultures of rat retinal explants exposed to NMDA, we found that NPY pretreatment prevented NMDA-induced cell death. However, in an animal model of retinal ischemia-reperfusion injury, pretreatment with NPY or (Leu31, Pro34)-NPY was not able to prevent apoptosis or rescue RGCs. In conclusion, we found modulatory effects of NPY application that for the first time were detected at the level of RGCs. However, further studies are needed to evaluate whether NPY neuroprotective actions detected in retinal explants can be translated into animal models of retinal degenerative diseases.

  14. Resistin, an Adipokine with Non-Generalized Actions on Sympathetic Nerve Activity

    PubMed Central

    Badoer, Emilio; Kosari, Samin; Stebbing, Martin J.

    2015-01-01

    The World Health Organization has called obesity a global epidemic. There is a strong association between body weight gain and blood pressure. A major determinant of blood pressure is the level of activity in sympathetic nerves innervating cardiovascular organs. A characteristic of obesity, in both humans and in animal models, is an increase in sympathetic nerve activity to the skeletal muscle vasculature and to the kidneys. Obesity is now recognized as a chronic, low level inflammatory condition, and pro-inflammatory cytokines are elevated including those produced by adipose tissue. The most well-known adipokine released from fat tissue is leptin. The adipokine, resistin, is also released from adipose tissue. Resistin can act in the central nervous system to influence the sympathetic nerve activity. Here, we review the effects of resistin on sympathetic nerve activity and compare them with leptin. We build an argument that resistin and leptin may have complex interactions. Firstly, they may augment each other as both are excitatory on sympathetic nerves innervating cardiovascular organs; In contrast, they could antagonize each other's actions on brown adipose tissue, a key metabolic organ. These interactions may be important in conditions in which leptin and resistin are elevated, such as in obesity. PMID:26617526

  15. Key programmatic steps and activities for implementing the Uranium Mill Tailings Remedial Action Project. [UMTRA Project

    SciTech Connect

    Not Available

    1985-07-01

    The Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA) was enacted based upon findings by Congress that uranium mill tailings located at active and inactive hazard to the public, and that protection of the public health, safety and welfare, and the regulations of interstate commerce, require that every reasonable effort be made to provide for the stabilization, disposal, and control in a safe and environmentally sound manner of such tailings in order to prevent or minimize radon diffusion into the environment and to prevent or minimize other environmental hazards from such tailings.'' A general understanding of the steps leading to elimination of the hazards associated with designated uranium mill tailings sites, and the parties involved in that effort, are presented in this document. A representative schedule is also presented in this document to show both program sequence and activity interdependence. Those activities that have the most potential to influence program duration, because of the significant amount of additional time that may be required, include identification and selection of a suitable site, field data collection delays due to weather, actual acquisition of the designated or alternate disposal site, construction delays due to weather, and site licensing. This document provides an understanding of the steps, the sequence, the parties involved, and a representative duration of activities leading to remedial action and cleanup at the designated inactive uranium mill tailings sites. 10 refs., 5 figs., 1 tab.

  16. Participating in politics resembles physical activity: general action patterns in international archives, United States archives, and experiments.

    PubMed

    Noguchi, Kenji; Handley, Ian M; Albarracín, Dolores

    2011-02-01

    A series of studies examined whether political participation can emerge from general patterns of indiscriminate activity. In the first two studies, general action tendencies were measured by combining national and state-level indicators of high activity (e.g., impulsiveness, pace of life, and physical activity) from international and U.S. data. This action-tendency index positively correlated with a measure of political participation that consisted of voting behaviors and participation in political demonstrations. The following two experimental studies indicated that participants exposed to action words (e.g., go, move) had stronger intentions to vote in an upcoming election and volunteered more time to make phone calls on behalf of a university policy than participants exposed to inaction words did (e.g., relax, stop). These studies suggest that political participation can be predicted from general tendencies toward activity present at the national and state levels, as well as from verbal prompts suggestive of activity. PMID:21177515

  17. Participating in Politics Resembles Physical Activity: General Action Patterns in International Archives, United States Archives, and Experiments

    PubMed Central

    Noguchi, Kenji; Handley, Ian M.; Albarracín, Dolores

    2016-01-01

    A series of studies examined whether political participation can emerge from general patterns of indiscriminate activity. In the first two studies, general action tendencies were measured by combining national and state-level indicators of high activity (e.g., impulsiveness, pace of life, and physical activity) from international and U.S. data. This action-tendency index positively correlated with a measure of political participation that consisted of voting behaviors and participation in political demonstrations. The following two experimental studies indicated that participants exposed to action words (e.g., go, move) had stronger intentions to vote in an upcoming election and volunteered more time to make phone calls on behalf of a university policy than participants exposed to inaction words did (e.g., relax, stop). These studies suggest that political participation can be predicted from general tendencies toward activity present at the national and state levels, as well as from verbal prompts suggestive of activity. PMID:21177515

  18. Data base management activities for the Remedial Action Program at ORNL: Calendar year 1988

    SciTech Connect

    Voorhees, L.D.; Hook, L.A.; Gentry, M.J.; McCord, R.A.; Faulkner, M.A.; Bledsoe, J.L.; Newman, K.A.; Owen, P.T.; Rosen, A.E.

    1989-04-01

    The ORNL Remedial Action Program (RAP) was established in 1985 in response to state and federal regulations mandating corrective actions at contaminated sites. To achieve this goal, numerous and varied studies are being conducted to characterize the type and extent of contamination. Environmental data collected in support of other programs at ORNL are also of use to RAP. Collectively, these studies are generating a voluminous amount of data. A computerized Data and Information Management System (DIMS) was developed for RAP to (1) provide a centralized repository for data pertinent to RAP and (2) provide support for the investigations and assessments leading to the long-term remediation of contaminated facilities and sites. The current DIMS and its role in supporting RAP during 1988 are described. The DIMS consists of three components: (1) the Numeric Data Base, (2) the Bibliographic Data Base, and (3) the Records Control Data Base. This report addresses all three data bases, but focuses on a description of the contents of the Numeric Data Base. The types of numeric data currently available are summarized in the tables and figures. More detailed information on the contents of the RAP Numeric Data Base has been assembled in a menu-driven format on IBM PC diskettes, which are available upon request. 6 refs.

  19. Central action of FGF19 reduces hypothalamic AGRP/NPY neuron activity and improves glucose metabolism.

    PubMed

    Marcelin, Geneviève; Jo, Young-Hwan; Li, Xiaosong; Schwartz, Gary J; Zhang, Ying; Dun, Nae J; Lyu, Rong-Ming; Blouet, Clémence; Chang, Jaw K; Chua, Streamson

    2014-02-01

    Tight control of glucose excursions has been a long-standing goal of treatment for patients with type 2 diabetes mellitus in order to ameliorate the morbidity and mortality associated with hyperglycemia. Fibroblast growth factor (FGF) 19 is a hormone-like enterokine released postprandially that emerged as a potential therapeutic agent for metabolic disorders, including diabetes and obesity. Remarkably, FGF19 treatment has hypoglycemic actions that remain potent in models of genetic and acquired insulin resistance. Here, we provided evidence that the central nervous system responds to FGF19 administered in the periphery. Then, in two mouse models of insulin resistance, leptin-deficiency and high-fat diet feeding, third intra-cerebro-ventricular infusions of FGF19 improved glycemic status, reduced insulin resistance and potentiated insulin signaling in the periphery. In addition, our study highlights a new mechanism of central FGF19 action, involving the suppression of AGRP/NPY neuronal activity. Overall, our work unveils novel regulatory pathways induced by FGF19 that will be useful in the design of novel strategies to control diabetes in obesity. PMID:24567901

  20. Action physics

    NASA Astrophysics Data System (ADS)

    McGinness, Lachlan P.; Savage, C. M.

    2016-09-01

    More than a decade ago, Edwin Taylor issued a "call to action" that presented the case for basing introductory university mechanics teaching around the principle of stationary action [E. F. Taylor, Am. J. Phys. 71, 423-425 (2003)]. We report on our response to that call in the form of an investigation of the teaching and learning of the stationary action formulation of physics in a first-year university course. Our action physics instruction proceeded from the many-paths approach to quantum physics to ray optics, classical mechanics, and relativity. Despite the challenges presented by action physics, students reported it to be accessible, interesting, motivational, and valuable.

  1. Investigating Seasonal Gravity Wave Activity in the Summer Polar Mesosphere

    NASA Astrophysics Data System (ADS)

    Zhao, Y.; Taylor, M. J.; Randall, C. E.; Lumpe, J. D., Jr.; Siskind, D. E.; Bailey, S. M.; Russell, J. M., III

    2014-12-01

    The NASA Aeronomy of Ice in the Mesosphere (AIM) satellite is the first satellite mission dedicated to studying polar mesospheric clouds (PMCs). In particular, the Cloud Imaging and Particle Size (CIPS) instrument onboard AIM obtains large-field PMC images enabling a unique investigation of the mesospheric gravity waves, as the satellite traverses over the summer polar regions. The high quality of CIPS data has provided an exceptional capability to investigate the gravity wave signatures in the summer polar mesosphere where previous measurements have been sparse. We have utilized 12 consecutive seasons of AIM/CIPS PMC albedo data to derive the statistical properties of a broad spectrum of gravity waves present in the PMC data. Over 60,000 waves with horizontal scale sizes ranging from ~50 to >2000 km were identified and measured, revealing a well-developed, consistent distribution for their horizontal wavelengths with a peak in occurrence frequency centered around 400 km. The same result was found from season to season and in both hemispheres. Throughout this study, the wave activity in the southern hemisphere was found to be 10-15% higher than in the northern hemisphere and both northern and southern wave activity was found to decrease systematically (average ~15%) during the course of each summer season. We present new results of the intra-seasonal, inter-annual and hemispheric variability of these waves observed over the whole summer polar cap regions. The systematic decrease in wave activity is consistent with background wind filtering in the northern hemisphere but is not apparently associated with the critical level filtering in the southern hemisphere.

  2. Investigation of MEK activity in COS7 cells entering mitosis.

    PubMed

    Shi, Huaiping; Zhang, Tianying; Yi, Yongqing; Luo, Jun

    2014-12-01

    Although the mitogen-activated protein kinase (MAPK) pathway has been extensively investigated, numerous events remain unclear. In the present study, we examined mitogen-activated protein kinase kinase (MEK) expression from interphase to mitosis. Following nocodazole treatment, COS7 cells gradually became round as early as 4 h after treatment. Cyclin B1 expression gradually increased from 4 to 24 h in the presence of nocodazole. When cells were treated with nocodazole for 4 h, the level of epidermal growth factor (EGF)-mediated MEK phosphorylation did not significantly change between nocodazole-untreated and -treated (4 h) cells (P>0.05). However, EGF-mediated MEK phosphorylation was significantly inhibited upon treatment with nocodazole for 8 and 24 h compared to nocodazole-untreated cells (P<0.05). MEK phosphorylation levels were comparable between 1, 5, 10 and 50 ng/ml EGF treatments. Phorbol 12-myristic 13-acetate (PMA) did not activate MEK in mitotic cells. Following treatment of COS7 cells at the interphase with AG1478 or U0126, MEK phosphorylation was blocked. In addition, the investigation of the expression of proteins downstream of MEK demonstrated that EGF does not significantly affect the phosphorylation level of extracellular-signal-regulated kinase (ERK), ribosomal protein S6 kinase (RSK) and Elk in mitotic cells (P>0.05). The results showed that MEK expression is gradually inhibited from cell interphase to mitosis, and that MEK downstream signaling is affected by this inhibition, which probably reflects the requirements of cell physiology during mitosis.

  3. Investigating seasonal gravity wave activity in the summer polar mesosphere

    NASA Astrophysics Data System (ADS)

    Zhao, Y.; Taylor, M. J.; Randall, C. E.; Lumpe, J. D.; Siskind, D. E.; Bailey, S. M.; Russell, J. M.

    2015-05-01

    The NASA Aeronomy of Ice in the Mesosphere (AIM) satellite is the first spaceborne mission dedicated to studying high-altitude (~83 km) Polar Mesospheric Clouds (PMCs). Since its launch in 2007, the Cloud Imaging and Particle Size (CIPS) instrument onboard AIM has obtained large-field, high resolution (25 km2/pixel) images of the PMCs, enabling a unique investigation of mesospheric gravity wave activity in the summer polar mesosphere where previous measurements have been sparse. In this study, we have analyzed 12 consecutive seasons of AIM/CIPS PMC albedo data to determine the statistical properties of medium and large horizontal scale (>100 km) gravity waves present in the PMC data. Over 60,000 wave events with horizontal scale-sizes ranging up to >2000 km have been identified and measured, revealing a wealth of wave events particularly in the ~300-800 km range where our analysis sensitivity is largest. These data are ideal for investigating the intra-seasonal, inter-annual and hemispheric variability of these waves as observed over the whole summer polar cap regions. Throughout this 6 year study, the wave activity in the southern hemisphere was found to be consistently 10-15% higher than in the northern hemisphere and both the northern and southern hemisphere wave activity was determined to decrease systematically (by ~15%) during the course of each summer season. This decrease agrees well with previous seasonal stratospheric studies of variations in the wave energy, suggesting a direct influence of the lower atmospheric sources on polar mesospheric dynamics. Very similar and consistent results were also found from season to season in both hemispheres providing new information for gravity wave modeling and dynamical studies of the high-latitude summer-time mesosphere.

  4. Active control: an investigation method for combustion instabilities

    NASA Astrophysics Data System (ADS)

    Poinsot, T.; Yip, B.; Veynante, D.; Trouvé, A.; Samaniego, J. M.; Candel, S.

    1992-07-01

    Closed-loop active control methods and their application to combustion instabilities are discussed. In these methods the instability development is impeded with a feedback control loop: the signal provided by a sensor monitoring the flame or pressure oscillations is processed and sent back to actuators mounted on the combustor or on the feeding system. Different active control systems tested on a non-premixed multiple-flame turbulent combustor are described. These systems can suppress all unstable plane modes of oscillation (i.e. low frequency modes). The active instability control (AIC) also constitutes an original and powerful technique for studies of mechanisms leading to instability or resulting from the instability. Two basic applications of this kind are described. In the first case the flame is initially controlled with AIC, the feedback loop is then switched off and the growth of the instability is analysed through high speed Schlieren cinematography and simultaneous sound pressure and reaction rate measurements. Three phases are identified during th growth of the oscillations: (1) a linear phase where acoustic waves induce a flapping motion of the flame sheets without interaction between sheets, (2) a modulation phase, where flame sheets interact randomly and (3) a nonlinear phase where the flame sheets are broken and a limit cycle is reached. In the second case we investigate different types of flame extinctions associated with combustion instability. It is shown that pressure oscillations may lead to partial or total extinctions. Extinctions occur in various forms but usually follow a rapid growth of pressure oscillations. The flame is extinguished during the modulation phase observed in the initiation experiments. In these studies devoted to transient instability phenomena, the control system constitutes a unique investigation tool because it is difficult to obtain the same information by other means. Implications for modelling and prediction of

  5. Seal Investigations of an Active Clearance Control System Concept

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M.; Taylor, Shawn; Oswald, Jay; DeCastro, Jonathan A.

    2006-01-01

    In an effort to improve upon current thermal active clearance control methods, a first generation, fast-acting mechanically actuated, active clearance control system has been designed and installed into a non-rotating test rig. In order to harvest the benefit of tighter blade tip clearances, low-leakage seals are required for the actuated carrier segments of the seal shroud to prevent excessive leakage of compressor discharge (P3) cooling air. The test rig was designed and fabricated to facilitate the evaluation of these types of seals, identify seal leakage sources, and test other active clearance control system concepts. The objective of this paper is to present both experimental and analytical investigations into the nature of the face-seal to seal-carrier interface. Finite element analyses were used to examine face seal contact pressures and edge-loading under multiple loading conditions, varied E-seal positions and two new face seal heights. The analyses indicated that moving the E-seal inward radially and reducing face seal height would lead to more uniform contact conditions between the face seal and the carriers. Lab testing confirmed that moving the balance diameter inward radially caused a decrease in overall system leakage.

  6. The Body Action Coding System II: muscle activations during the perception and expression of emotion

    PubMed Central

    Huis In ‘t Veld, Elisabeth M. J.; van Boxtel, Geert J. M.; de Gelder, Beatrice

    2014-01-01

    Research into the expression and perception of emotions has mostly focused on facial expressions. Recently, body postures have become increasingly important in research, but knowledge on muscle activity during the perception or expression of emotion is lacking. The current study continues the development of a Body Action Coding System (BACS), which was initiated in a previous study, and described the involvement of muscles in the neck, shoulders and arms during expression of fear and anger. The current study expands the BACS by assessing the activity patterns of three additional muscles. Surface electromyography of muscles in the neck (upper trapezius descendens), forearms (extensor carpi ulnaris), lower back (erector spinae longissimus) and calves (peroneus longus) were measured during active expression and passive viewing of fearful and angry body expressions. The muscles in the forearm were strongly active for anger expression and to a lesser extent for fear expression. In contrast, muscles in the calves were recruited slightly more for fearful expressions. It was also found that muscles automatically responded to the perception of emotion, without any overt movement. The observer's forearms responded to the perception of fear, while the muscles used for leaning backwards were activated when faced with an angry adversary. Lastly, the calf responded immediately when a fearful person was seen, but responded slower to anger. There is increasing interest in developing systems that are able to create or recognize emotional body language for the development of avatars, robots, and online environments. To that end, multiple coding systems have been developed that can either interpret or create bodily expressions based on static postures, motion capture data or videos. However, the BACS is the first coding system based on muscle activity. PMID:25294993

  7. Opposing actions of endocannabinoids on cholangiocarcinoma growth is via the differential activation of Notch signaling

    SciTech Connect

    Frampton, Gabriel; Coufal, Monique; Li, Huang; Ramirez, Jonathan; DeMorrow, Sharon

    2010-05-15

    The endocannabinoids anandamide (AEA) and 2-arachidonylglycerol (2-AG) have opposing effects on cholangiocarcinoma growth. Implicated in cancer, Notch signaling requires the {gamma}-secretase complex for activation. The aims of this study were to determine if the opposing effects of endocannabinoids depend on the differential activation of the Notch receptors and to demonstrate that the differential activation of these receptors are due to presenilin 1 containing- and presenilin 2 containing-{gamma}-secretase complexes. Mz-ChA-1 cells were treated with AEA or 2-AG. Notch receptor expression, activation, and nuclear translocation were determined. Specific roles for Notch 1 and 2 on cannabinoid-induced effects were determined by transient transfection of Notch 1 or 2 shRNA vectors before stimulation with AEA or 2-AG. Expression of presenilin 1 and 2 was determined after AEA or 2-AG treatment, and the involvement of presenilin 1 and 2 in the cannabinoid-induced effects was demonstrated in cell lines with low presenilin 1 or 2 expression. Antiproliferative effects of AEA required increased Notch 1 mRNA, activation, and nuclear translocation, whereas the growth-promoting effects induced by 2-AG required increased Notch 2 mRNA expression, activation, and nuclear translocation. AEA increased presenilin 1 expression and recruitment into the {gamma}-secretase complex, whereas 2-AG increased expression and recruitment of presenilin 2. The development of novel therapeutic strategies aimed at modulating the endocannabinoid system or mimicking the mode of action of AEA on Notch signaling pathways would prove beneficial for cholangiocarcinoma management.

  8. The Body Action Coding System II: muscle activations during the perception and expression of emotion.

    PubMed

    Huis In 't Veld, Elisabeth M J; van Boxtel, Geert J M; de Gelder, Beatrice

    2014-01-01

    Research into the expression and perception of emotions has mostly focused on facial expressions. Recently, body postures have become increasingly important in research, but knowledge on muscle activity during the perception or expression of emotion is lacking. The current study continues the development of a Body Action Coding System (BACS), which was initiated in a previous study, and described the involvement of muscles in the neck, shoulders and arms during expression of fear and anger. The current study expands the BACS by assessing the activity patterns of three additional muscles. Surface electromyography of muscles in the neck (upper trapezius descendens), forearms (extensor carpi ulnaris), lower back (erector spinae longissimus) and calves (peroneus longus) were measured during active expression and passive viewing of fearful and angry body expressions. The muscles in the forearm were strongly active for anger expression and to a lesser extent for fear expression. In contrast, muscles in the calves were recruited slightly more for fearful expressions. It was also found that muscles automatically responded to the perception of emotion, without any overt movement. The observer's forearms responded to the perception of fear, while the muscles used for leaning backwards were activated when faced with an angry adversary. Lastly, the calf responded immediately when a fearful person was seen, but responded slower to anger. There is increasing interest in developing systems that are able to create or recognize emotional body language for the development of avatars, robots, and online environments. To that end, multiple coding systems have been developed that can either interpret or create bodily expressions based on static postures, motion capture data or videos. However, the BACS is the first coding system based on muscle activity.

  9. Data base management activities for the Remedial Action Program at ORNL, calendar year 1987

    SciTech Connect

    Voorhees, L.D.; Hook, L.A.; Gentry, M.J.; McCord, R.A.; Faulkner, M.A.; Newman, K.A.; Owen, P.T.

    1988-05-01

    The Oak Ridge National Laboratory (ORNL) Remedial Action Program (RAP) was established in FY 1985 to apply corrective measures at areas contaminated with radioactive and/or hazardous chemical wastes. To achieve this goal, numerous and varied studies are being conducted to characterize the waste disposal sites. Environmental data collected in support of other programs at ORNL are also of use to RAP. These studies are generating a voluminous amount of data on a scale unprecedented for ORNL. A computerized Data and Information Management System (DIMS) was developed for RAP to (1) provide a centralized repository for data pertinent to RAP and (2) provide support for the investigations and assessments leading to the long-term remediation of contaminated facilities and sites. 10 refs., 25 figs., 16 tabs.

  10. Cardiovascular Actions and Therapeutic Potential of Tetramethylpyrazine (Active Component Isolated from Rhizoma Chuanxiong): Roles and Mechanisms

    PubMed Central

    Guo, Ming; Liu, Yue; Shi, Dazhuo

    2016-01-01

    Tetramethylpyrazine (TMP), a pharmacologically active component isolated from the rhizome of the Chinese herb Rhizoma Chuanxiong (Chuanxiong), has been clinically used in China and Southeast Asian countries for the prevention and treatment of cardiovascular diseases (CVDs) for about fifty years. The pharmacological effects of TMP on the cardiovascular system have attracted great interest. Emerging experimental studies and clinical trials have demonstrated that TMP prevents atherosclerosis as well as ischemia-reperfusion injury. The cardioprotective effects of TMP are mainly related to its antioxidant, anti-inflammatory, or calcium-homeostasis effects. This review focuses on the roles and mechanisms of action of TMP in the cardiovascular system and provides a novel perspective on TMP's clinical use. PMID:27314011

  11. Cardiovascular Actions and Therapeutic Potential of Tetramethylpyrazine (Active Component Isolated from Rhizoma Chuanxiong): Roles and Mechanisms.

    PubMed

    Guo, Ming; Liu, Yue; Shi, Dazhuo

    2016-01-01

    Tetramethylpyrazine (TMP), a pharmacologically active component isolated from the rhizome of the Chinese herb Rhizoma Chuanxiong (Chuanxiong), has been clinically used in China and Southeast Asian countries for the prevention and treatment of cardiovascular diseases (CVDs) for about fifty years. The pharmacological effects of TMP on the cardiovascular system have attracted great interest. Emerging experimental studies and clinical trials have demonstrated that TMP prevents atherosclerosis as well as ischemia-reperfusion injury. The cardioprotective effects of TMP are mainly related to its antioxidant, anti-inflammatory, or calcium-homeostasis effects. This review focuses on the roles and mechanisms of action of TMP in the cardiovascular system and provides a novel perspective on TMP's clinical use. PMID:27314011

  12. Nouns, verbs, objects, actions, and abstractions: Local fMRI activity indexes semantics, not lexical categories

    PubMed Central

    Moseley, Rachel L.; Pulvermüller, Friedemann

    2014-01-01

    Noun/verb dissociations in the literature defy interpretation due to the confound between lexical category and semantic meaning; nouns and verbs typically describe concrete objects and actions. Abstract words, pertaining to neither, are a critical test case: dissociations along lexical-grammatical lines would support models purporting lexical category as the principle governing brain organisation, whilst semantic models predict dissociation between concrete words but not abstract items. During fMRI scanning, participants read orthogonalised word categories of nouns and verbs, with or without concrete, sensorimotor meaning. Analysis of inferior frontal/insula, precentral and central areas revealed an interaction between lexical class and semantic factors with clear category differences between concrete nouns and verbs but not abstract ones. Though the brain stores the combinatorial and lexical-grammatical properties of words, our data show that topographical differences in brain activation, especially in the motor system and inferior frontal cortex, are driven by semantics and not by lexical class. PMID:24727103

  13. Nouns, verbs, objects, actions, and abstractions: local fMRI activity indexes semantics, not lexical categories.

    PubMed

    Moseley, Rachel L; Pulvermüller, Friedemann

    2014-05-01

    Noun/verb dissociations in the literature defy interpretation due to the confound between lexical category and semantic meaning; nouns and verbs typically describe concrete objects and actions. Abstract words, pertaining to neither, are a critical test case: dissociations along lexical-grammatical lines would support models purporting lexical category as the principle governing brain organisation, whilst semantic models predict dissociation between concrete words but not abstract items. During fMRI scanning, participants read orthogonalised word categories of nouns and verbs, with or without concrete, sensorimotor meaning. Analysis of inferior frontal/insula, precentral and central areas revealed an interaction between lexical class and semantic factors with clear category differences between concrete nouns and verbs but not abstract ones. Though the brain stores the combinatorial and lexical-grammatical properties of words, our data show that topographical differences in brain activation, especially in the motor system and inferior frontal cortex, are driven by semantics and not by lexical class.

  14. [European innovation partnership on active and healthy aging: moving from policy to action].

    PubMed

    García Lizana, Francisca

    2013-01-01

    Demographic change and aging are a common challenge in Europe. The rising number of elderly people will need support at home, and will consume more healthcare services, putting further pressure on the welfare system. Collaborative, integrated and people-centered care provision, whether in hospitals, homes or in the community, is a way forward to sustainable and efficient care systems. Innovative treatments to address chronic diseases and the functional decline of older people will enable them to live longer in better health and with a better quality of life. To fully unleash the potential of aging in the European Union, the European Commission -within its Innovation Union policy- launched the first European Innovation Partnership on Active and Healthy Ageing (EIP AHA). Promoting engagement and partnerships among all stakeholders in the healthcare chain is essential. This article describes the theoretical foundations, the development and expectations of the initiative, and its first actions.

  15. Participatory action research: practical strategies for actively engaging and maintaining participation in immigrant and refugee communities.

    PubMed

    van der Velde, Jeannette; Williamson, Deanna L; Ogilvie, Linda D

    2009-09-01

    In this research we examined the processes involved in implementing and maintaining a participatory action research (PAR) project by uncovering how theoretical PAR tenets hold up in the reality of a community-based project addressing immigrants' and refugees' mental health needs. Qualitative data from focus groups with these newcomers were analyzed for thematic content. Findings reveal that active participation is seen as the gateway into a PAR project, whereas knowledge attainment and empowerment are the stimuli for continued participation. The data also suggest that newcomers' motivations to participate in a PAR-oriented project might vary across ethno-cultural groups. Practitioners working in community-based initiatives would do well to appeal to the diversity of motivational factors, while endorsing individual and group strengths.

  16. Investigation of Cytotoxic Activity in Four Stachys Species from Iran

    PubMed Central

    Khanavi, Mahnaz; Manayi, Azadeh; Lotfi, Mahnaz; Abbasi, Rofeyde; Majdzadeh, Maryam; Ostad, Seyed Nasser

    2012-01-01

    The aerial parts of Stachys laxa Boiss. and Buhse. from Siah-bishe in Mazandaran province, Stachys trinervis Aitch. and Hemsl. from Karaj in Alborz province, Stachys subaphylla Rech. F. and Stachys turcomanica Trautv. from Golestan province have been collected in May 2008. Total extracts were obtained through MeOH/H2O (80/20) and then partitioned between CHCl3, EtOAc and MeOH. These fractions and total extracts have been investigated for in-vitro cytotoxic activity against the colon carcinoma (HT-29), colorectal adenocarcinoma (Caco-2), breast ductal carcinoma (T47D) and Swiss mouse embryo fibroblast (NIH 3T3) cell lines using MTT assay (3-(4,5-di methyl thiazol-2-yl)-2,5-di phenyltetrazolium bromide). At each cell line, doses of 3.125, 6.25, 12.5, 25, 100, 200, 400 and 800 µg/mL in 1% (v/v) DMSO of all samples were tested. Ethyl acetate and chloroform fractions of Stachys laxa against proliferation of T47D and HT-29 cell lines and chloroform fraction of Stachys subaphylla and Stachys subaphylla ethyl acetate fraction toward T47D cell line exhibited highest cytotoxic activity (IC50 < 50 µg/mL). Ethyl acetate and chloroform fractions of Stachys turcomanica against HT-29 cell line, except methanol fraction of Stachys subaphylla, the other extrcts on T47D cell line, represented moderate cytotoxic activity (IC50 < 70 µg/mL). All fractions of S. trinervis demonstrated no effective cytotoxic activity. IC50 values confirmed that the growth and proliferation of HT-29 and T47D cells were most affected by chloroform and ethyl acetate fractions of Stachys laxa and Stachys turcomanica due to their nonpolar compounds. PMID:24250483

  17. Hemodynamic actions of systemically injected pituitary adenylate cyclase activating polypeptide-27 in the rat

    NASA Technical Reports Server (NTRS)

    Whalen, E. J.; Johnson, A. K.; Lewis, S. J.

    1999-01-01

    The aims of this study were (1) to characterize the hemodynamic mechanisms underlying the hypotensive effects of pituitary adenylate cyclase activating polypeptide-27 (PACAP-27 0.1-2.0 nmol/kg, i.v.) in pentobarbital-anesthetized rats, and (2) to determine the roles of the autonomic nervous system, adrenal catecholamines and endothelium-derived nitric oxide (NO) in the expression of PACAP-27-mediated effects on hemodynamic function. PACAP-27 produced dose-dependent decreases in mean arterial blood pressure and hindquarter and mesenteric vascular resistances in saline-treated rats. PACAP-27 also produced pronounced falls in mean arterial blood pressure in rats treated with the ganglion blocker, chlorisondamine (5 mg/kg, i.v.). The hypotensive and vasodilator actions of PACAP-27 were not attenuated by the beta-adrenoceptor antagonist, propranolol (1 mg/kg, i.v.), or the NO synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME 50 micromol/kg, i.v.). PACAP-27 produced dose-dependent increases in heart rate whereas the hypotensive response produced by the nitrovasodilator, sodium nitroprusside (10 microg/kg, i.v.), was associated with a minimal tachycardia. The PACAP-27-induced tachycardia was unaffected by chlorisondamine, but was virtually abolished by propranolol. These results suggest that the vasodilator effects of PACAP-27 are due to actions in the microcirculation rather than to the release of adrenal catecholamines and that this vasodilation may not involve the release of endothelium-derived NO. These results also suggest that PACAP-27 produces tachycardia by directly releasing norepinephrine from cardiac sympathetic nerve terminals rather than by direct or baroreceptor reflex-mediated increases in sympathetic nerve activity.

  18. Experimental Investigation of the Root Cause Mechanism and Effectiveness of Mitigating Actions for Axial Offset Anomaly in Pressurized Water Reactors

    SciTech Connect

    Said Abdel-Khalik

    2005-07-02

    Axial offset anomaly (AOA) in pressurized water reactors refers to the presence of a significantly larger measured negative axial offset deviation than predicted by core design calculations. The neutron flux depression in the upper half of high-power rods experiencing significant subcooled boiling is believed to be caused by the concentration of boron species within the crud layer formed on the cladding surface. Recent investigations of the root-cause mechanism for AOA [1,2] suggest that boron build-up on the fuel is caused by precipitation of lithium metaborate (LiBO2) within the crud in regions of subcooled boiling. Indirect evidence in support of this hypothesis was inferred from operating experience at Callaway, where lithium return and hide-out were, respectively, observed following power reductions and power increases when AOA was present. However, direct evidence of lithium metaborate precipitation within the crud has, heretofore, not been shown because of its retrograde solubility. To this end, this investigation has been undertaken in order to directly verify or refute the proposed root-cause mechanism of AOA, and examine the effectiveness of possible mitigating actions to limit its impact in high power PWR cores.

  19. EXPERIMENTAL STRATEGIES FOR INVESTIGATING PSYCHOSTIMULANT DRUG ACTIONS AND PREFRONTAL CORTICAL FUNCTON IN ADHD AND RELATED ATTENTION DISORDERS

    PubMed Central

    Agster, K. L.; Clark, B. D.; Gao, W-J.; Shumsky, J. S.; Wang, H-X.; Berridge, C. W.; Waterhouse, B. D.

    2011-01-01

    Amphetamine-like psychostimulant drugs have been used for decades to treat a variety of clinical conditions. Methylphenidate (MPH) - RitalinR, a compound that blocks reuptake of synaptically released norepinephrine (NE) and dopamine (DA) in the brain, has been used for more than 30 years in low dose, long-term regimens to treat attention deficit-hyperactive disorder (ADHD) in juveniles, adolescents, and adults. These agents are now also becoming increasingly popular among healthy individuals from all walks of life (e.g. military, students) and age groups (teenagers thru senior citizens) to promote wakefulness and improve attention. While there is agreement regarding the primary biochemical action of MPH, the physiological basis for its efficacy in normal individuals and ADHD patients is lacking. Study of the behavioral and physiological actions of clinically and behaviorally relevant doses of MPH in normal animals provides an opportunity to explore the role of catecholamine transmitters in prefrontal cortical function and attentional processes as they relate to normal operation of brain circuits and ADHD pathology. The goal of ongoing studies has been to: 1) assess the effects of low dose MPH on rodent performance in a well characterized sensory-guided sustained attention task, 2) examine the effects of the same low-dose chronic MPH administration on task-related discharge of prefrontal cortical (PFC) neurons and 3) investigate the effects of NE and DA on membrane response properties and synaptic transmission in identified subsets of PFC neurons. Combinations of these approaches can be used in adolescent, adult and aged animals to identify the parameters of cell and neural circuit function that are regulated by MPH and to establish an overarching explanation of how MPH impacts PFC operations from cellular through behavioral functional domains. PMID:21901844

  20. Anesthetic action on extra-synaptic receptors: effects in neural population models of EEG activity

    PubMed Central

    Hashemi, Meysam; Hutt, Axel; Sleigh, Jamie

    2014-01-01

    The role of extra-synaptic receptors in the regulation of excitation and inhibition in the brain has attracted increasing attention. Because activity in the extra-synaptic receptors plays a role in regulating the level of excitation and inhibition in the brain, they may be important in determining the level of consciousness. This paper reviews briefly the literature on extra-synaptic GABA and NMDA receptors and their affinity to anesthetic drugs. We propose a neural population model that illustrates how the effect of the anesthetic drug propofol on GABAergic extra-synaptic receptors results in changes in neural population activity and the electroencephalogram (EEG). Our results show that increased tonic inhibition in inhibitory cortical neurons cause a dramatic increase in the power of both δ− and α− bands. Conversely, the effects of increased tonic inhibition in cortical excitatory neurons and thalamic relay neurons have the opposite effect and decrease the power in these bands. The increased δ-activity is in accord with observed data for deepening propofol anesthesia; but is absolutely dependent on the inclusion of extrasynaptic (tonic) GABA action in the model. PMID:25540612

  1. Statins meditate anti-atherosclerotic action in smooth muscle cells by peroxisome proliferator-activated receptor-γ activation

    SciTech Connect

    Fukuda, Kazuki; Matsumura, Takeshi; Senokuchi, Takafumi; Ishii, Norio; Kinoshita, Hiroyuki; Yamada, Sarie; Murakami, Saiko; Nakao, Saya; Motoshima, Hiroyuki; Kondo, Tatsuya; Kukidome, Daisuke; Kawasaki, Shuji; Kawada, Teruo; Nishikawa, Takeshi; Araki, Eiichi

    2015-01-30

    Highlights: • Statins induce PPARγ activation in vascular smooth muscle cells. • Statin-induced PPARγ activation is mediated by COX-2 expression. • Statins suppress cell migration and proliferation in vascular smooth muscle cells. • Statins inhibit LPS-induced inflammatory responses by PPARγ activation. • Fluvastatin suppress the progression of atherosclerosis and induces PPARγ activation in the aorta of apoE-deficient mice. - Abstract: The peroxisome proliferator-activated receptor-γ (PPARγ) is an important regulator of lipid and glucose metabolism, and its activation is reported to suppress the progression of atherosclerosis. We have reported that 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) activate PPARγ in macrophages. However, it is not yet known whether statins activate PPARγ in other vascular cells. In the present study, we investigated whether statins activate PPARγ in smooth muscle cells (SMCs) and endothelial cells (ECs) and thus mediate anti-atherosclerotic effects. Human aortic SMCs (HASMCs) and human umbilical vein ECs (HUVECs) were used in this study. Fluvastatin and pitavastatin activated PPARγ in HASMCs, but not in HUVECs. Statins induced cyclooxygenase-2 (COX-2) expression in HASMCs, but not in HUVECs. Moreover, treatment with COX-2-siRNA abrogated statin-mediated PPARγ activation in HASMCs. Statins suppressed migration and proliferation of HASMCs, and inhibited lipopolysaccharide-induced expression of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) in HASMCs. These effects of statins were abrogated by treatment with PPARγ-siRNA. Treatment with statins suppressed atherosclerotic lesion formation in Apoe{sup −/−} mice. In addition, transcriptional activity of PPARγ and CD36 expression were increased, and the expression of MCP-1 and TNF-α was decreased, in the aorta of statin-treated Apoe{sup −/−} mice. In conclusion, statins mediate anti-atherogenic effects

  2. Primary somatosensory contribution to action observation brain activity-combining fMRI and cTBS.

    PubMed

    Valchev, Nikola; Gazzola, Valeria; Avenanti, Alessio; Keysers, Christian

    2016-08-01

    Traditionally the mirror neuron system (MNS) only includes premotor and posterior parietal cortices. However, somatosensory cortices, BA1/2 in particular, are also activated during action execution and observation. Here, we examine whether BA1/2 and the parietofrontal MNS integrate information by using functional magnetic resonance imaging (fMRI)-guided continuous theta-burst stimulation (cTBS) to perturb BA1/2. Measuring brain activity using fMRI while participants are under the influence of cTBS shows local cTBS effects in BA1/2 varied, with some participants showing decreases and others increases in the BOLD response to viewing actions vs control stimuli. We show how measuring cTBS effects using fMRI can harness this variance using a whole-brain regression. This analysis identifies brain regions exchanging action-specific information with BA1/2 by mapping voxels away from the coil with cTBS-induced, action-observation-specific BOLD contrast changes that mirror those under the coil. This reveals BA1/2 exchanges action-specific information with premotor, posterior parietal and temporal nodes of the MNS during action observation. Although anatomical connections between BA1/2 and these regions are well known, this is the first demonstration that these connections carry action-specific signals during observation and hence, that BA1/2 plays a causal role in the human MNS. PMID:26979966

  3. Broad spectrum antibacterial and antifungal polymeric paint materials: synthesis, structure-activity relationship, and membrane-active mode of action.

    PubMed

    Hoque, Jiaul; Akkapeddi, Padma; Yadav, Vikas; Manjunath, Goutham B; Uppu, Divakara S S M; Konai, Mohini M; Yarlagadda, Venkateswarlu; Sanyal, Kaustuv; Haldar, Jayanta

    2015-01-28

    Microbial attachment and subsequent colonization onto surfaces lead to the spread of deadly community-acquired and hospital-acquired (nosocomial) infections. Noncovalent immobilization of water insoluble and organo-soluble cationic polymers onto a surface is a facile approach to prevent microbial contamination. In the present study, we described the synthesis of water insoluble and organo-soluble polymeric materials and demonstrated their structure-activity relationship against various human pathogenic bacteria including drug-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), and beta lactam-resistant Klebsiella pneumoniae as well as pathogenic fungi such as Candida spp. and Cryptococcus spp. The polymer coated surfaces completely inactivated both bacteria and fungi upon contact (5 log reduction with respect to control). Linear polymers were more active and found to have a higher killing rate than the branched polymers. The polymer coated surfaces also exhibited significant activity in various complex mammalian fluids such as serum, plasma, and blood and showed negligible hemolysis at an amount much higher than minimum inhibitory amounts (MIAs). These polymers were found to have excellent compatibility with other medically relevant polymers (polylactic acid, PLA) and commercial paint. The cationic hydrophobic polymer coatings disrupted the lipid membrane of both bacteria and fungi and thus showed a membrane-active mode of action. Further, bacteria did not develop resistance against these membrane-active polymers in sharp contrast to conventional antibiotics and lipopeptides, thus the polymers hold great promise to be used as coating materials for developing permanent antimicrobial paint. PMID:25541751

  4. Broad spectrum antibacterial and antifungal polymeric paint materials: synthesis, structure-activity relationship, and membrane-active mode of action.

    PubMed

    Hoque, Jiaul; Akkapeddi, Padma; Yadav, Vikas; Manjunath, Goutham B; Uppu, Divakara S S M; Konai, Mohini M; Yarlagadda, Venkateswarlu; Sanyal, Kaustuv; Haldar, Jayanta

    2015-01-28

    Microbial attachment and subsequent colonization onto surfaces lead to the spread of deadly community-acquired and hospital-acquired (nosocomial) infections. Noncovalent immobilization of water insoluble and organo-soluble cationic polymers onto a surface is a facile approach to prevent microbial contamination. In the present study, we described the synthesis of water insoluble and organo-soluble polymeric materials and demonstrated their structure-activity relationship against various human pathogenic bacteria including drug-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), and beta lactam-resistant Klebsiella pneumoniae as well as pathogenic fungi such as Candida spp. and Cryptococcus spp. The polymer coated surfaces completely inactivated both bacteria and fungi upon contact (5 log reduction with respect to control). Linear polymers were more active and found to have a higher killing rate than the branched polymers. The polymer coated surfaces also exhibited significant activity in various complex mammalian fluids such as serum, plasma, and blood and showed negligible hemolysis at an amount much higher than minimum inhibitory amounts (MIAs). These polymers were found to have excellent compatibility with other medically relevant polymers (polylactic acid, PLA) and commercial paint. The cationic hydrophobic polymer coatings disrupted the lipid membrane of both bacteria and fungi and thus showed a membrane-active mode of action. Further, bacteria did not develop resistance against these membrane-active polymers in sharp contrast to conventional antibiotics and lipopeptides, thus the polymers hold great promise to be used as coating materials for developing permanent antimicrobial paint.

  5. Incidental and Context-Responsive Activation of Structure- and Function-Based Action Features during Object Identification

    ERIC Educational Resources Information Center

    Lee, Chia-lin; Middleton, Erica; Mirman, Daniel; Kalenine, Solene; Buxbaum, Laurel J.

    2013-01-01

    Previous studies suggest that action representations are activated during object processing, even when task-irrelevant. In addition, there is evidence that lexical-semantic context may affect such activation during object processing. Finally, prior work from our laboratory and others indicates that function-based ("use") and structure-based…

  6. Administrators in Action--Managing Public Monies and Processing Emotion in School Activities: A Teaching Case Study

    ERIC Educational Resources Information Center

    Tenuto, Penny L.; Gardiner, Mary E.; Yamamoto, Julie K.

    2015-01-01

    This teaching case describes school administrators in action performing day-to-day leadership tasks, managing public funds in school activities, and interacting with others appropriately. The case focuses on administrative challenges in handling and managing school activity funds. A method for processing emotion is discussed to assist…

  7. Quality investigation of hydroxyprogesterone caproate active pharmaceutical ingredient and injection

    PubMed Central

    Chollet, John L.; Jozwiakowski, Michael J.

    2012-01-01

    The purpose of this study was to investigate the quality of hydroxyprogesterone caproate (HPC) active pharmaceutical ingredient (API) sources that may be used by compounding pharmacies, compared to the FDA-approved source of the API; and to investigate the quality of HPC injection samples obtained from compounding pharmacies in the US, compared to the FDA-approved product (Makena®). Samples of API were obtained from every source confirmed to be an original manufacturer of the drug for human use, which were all companies in China that were not registered with FDA. Eight of the ten API samples (80%) did not meet the impurity specifications required by FDA for the API used in the approved product. One API sample was found to not be HPC at all; additional laboratory testing showed that it was glucose. Thirty samples of HPC injection obtained from com pounding pharmacies throughout the US were also tested, and eight of these samples (27%) failed to meet the potency requirement listed in the USP monograph for HPC injection and/or the HPLC assay. Sixteen of the thirty injection samples (53%) exceeded the impurity limit setforthe FDA-approved drug product. These results confirm the inconsistency of compounded HPC Injections and suggest that the risk-benefit ratio of using an unapproved compounded preparation, when an FDA-approved drug product is available, is not favorable. PMID:22329865

  8. The conceptual maps in the development of the course of biology of tenth degree: An investigation experience action in the classroom

    NASA Astrophysics Data System (ADS)

    Samo Goyco, Marisol

    This investigation describes and combines the qualitative and quantitative methods of nature. The research I have work explore, observe, record and also it describes the experience to consider the education and teaching of the course. This investigation is a research that our students from the biology course, since the constructivist approach identifying and correct mistake. In this investigation there were participating twenty five students of tenth grade from a public school specialized in music. This research includes conceptual maps, computer integration, science programmed, internet, and broadcast and assessment approach. The research of conceptual maps establishes the correct method to perform capture the acknowledgement and attention of the investigators and the students which represents a significant relation between the concepts. Thought the investigator sustains on the cycle spiral of Carr and kemmis (1988) I design every unit considering the previous ideology of the student and elaborating the unit plan. Sustaining Maintain the methodology of the action research. The methodology has response to a new teaching paradigm. Situate as a principal assignment of the professor to contribute in the process of active learning to the students. Also helps to have in this process a reflection in their function or goals. During the research I analyze and wrote the observation and materials. The investigator express in her writing the final findings in every cycle. Also evaluates the map concepts the varied integration of activity and the assessment skills which are used through the socialized discussion. The socialized discussion communicates the participant concepts that should be attended. The students express between their peers and in front of the research of the investigator how they felt in terms of resources and the development of the maps. At the moment of this information I design the next cycle responding to the outstanding needs, this reflection genre a

  9. Intentional action processing results from automatic bottom-up attention: An EEG-investigation into the Social Relevance Hypothesis using hypnosis.

    PubMed

    Neufeld, Eleonore; Brown, Elliot C; Lee-Grimm, Sie-In; Newen, Albert; Brüne, Martin

    2016-05-01

    Social stimuli grab our attention. However, it has rarely been investigated how variations in attention affect the processing of social stimuli, although the answer could help us uncover details of social cognition processes such as action understanding. In the present study, we examined how changes to bottom-up attention affects neural EEG-responses associated with intentional action processing. We induced an increase in bottom-up attention by using hypnosis. We recorded the electroencephalographic μ-wave suppression of hypnotized participants when presented with intentional actions in first and third person perspective in a video-clip paradigm. Previous studies have shown that the μ-rhythm is selectively suppressed both when executing and observing goal-directed motor actions; hence it can be used as a neural signal for intentional action processing. Our results show that neutral hypnotic trance increases μ-suppression in highly suggestible participants when they observe intentional actions. This suggests that social action processing is enhanced when bottom-up attentional processes are predominant. Our findings support the Social Relevance Hypothesis, according to which social action processing is a bottom-up driven attentional process, and can thus be altered as a function of bottom-up processing devoted to a social stimulus. PMID:26998562

  10. Using discrepant events in science demonstrations to promote student engagement in scientific investigations: An action research study

    NASA Astrophysics Data System (ADS)

    Mancuso, Vincent J.

    Students' scientific investigations have been identified in national standards and related reform documents as a critical component of students' learning experiences in school, yet it is not easy to implement them in science classrooms. Could science demonstrations help science teachers put this recommendation into practice? While demonstrations are a common practice in the science classroom and research has documented some positive effects in terms of student motivation and engagement from their use, the literature also shows that, as traditionally presented, science demonstrations do not always achieve their intended outcomes. This, in turn, suggested the value of investigating what design elements of demonstrations could be used to promote specific instructional goals. Employing action research as a methodology, the proposed study was developed to explore how science demonstrations can be designed so as to most effectively promote student engagement in scientific investigations. More specifically, I was interested in examining the effects of using a discrepant event as part of the demonstration, as a way to create cognitive conflict and, thus, increase interest and engagement. I also investigated the relative merit of the well-researched POE (Predict, Observe, Explain) design versus employing demonstrations that appear to the student to be unplanned (what I will refer to as NOE, or a Naturally Occurring Experience). This study was informed by Constructivism, Situated Cognition and Conceptual Change as theoretical frameworks. The project included the design, implementation and study of an intervention consisting of three instructional units designed to support students' learning of the concepts of density, molecular arrangement of gas particles, and cohesion, respectively. In each of these units, lasting a total of two 80-minute class periods, students were asked to design and conduct an investigation to gain a better understanding of the concept under study. In

  11. Antibodies with beta-adrenergic activity from chronic chagasic patients modulate the QT interval and M cell action potential duration

    PubMed Central

    Medei, Emiliano Horacio; Nascimento, José H.M.; Pedrosa, Roberto C.; Barcellos, Luciane; Masuda, Masako O.; Sicouri, Serge; Elizari, Marcelo V.; Campos de Carvalho, Antonio C.

    2009-01-01

    Aims The aim of this study was to investigate whether the sera from chronic chagasic patients (CChPs) with beta-1 adrenergic activity (Ab-β) can modulate ventricular repolarization. Beta-adrenergic activity has been described in CChP. It increases the L-type calcium current and heart rate in isolated hearts, but its effects on ventricular repolarization has not been described. Methods and results In isolated rabbit hearts, under pacing condition, QT interval was measured under Ab-β perfusion. Beta-adrenergic activity was also tested in guinea pig ventricular M cells. Furthermore, the immunoglobulin fraction (IgG-β) of the Ab-β was tested on Ito, ICa, and Iks currents in rat, rabbit, and guinea pig myocytes, respectively. Beta-adrenergic activity shortened the QT interval. This effect was abolished in the presence of propranolol. In addition, sera from CChP without beta-adrenergic activity (Ab-β) did not modulate QT interval. The M cell action potential duration (APD) was reversibly shortened by Ab-β. Atenolol inhibited this effect of Ab-β, and Ab- did not modulate the AP of M cells. Ito was not modulated by isoproterenol nor by IgG-β. However, IgG-β increased ICa and IKs. Conclusion The shortening of the QT interval and APD in M cells and the increase of IKs and ICa induced by IgG-β contribute to repolarization changes that may trigger malignant ventricular arrhythmias observed in patients with chronic chagasic or idiopathic cardiomyopathy. PMID:18515284

  12. Stereoscopically Observing Manipulative Actions.

    PubMed

    Ferri, S; Pauwels, K; Rizzolatti, G; Orban, G A

    2016-08-01

    The purpose of this study was to investigate the contribution of stereopsis to the processing of observed manipulative actions. To this end, we first combined the factors "stimulus type" (action, static control, and dynamic control), "stereopsis" (present, absent) and "viewpoint" (frontal, lateral) into a single design. Four sites in premotor, retro-insular (2) and parietal cortex operated specifically when actions were viewed stereoscopically and frontally. A second experiment clarified that the stereo-action-specific regions were driven by actions moving out of the frontoparallel plane, an effect amplified by frontal viewing in premotor cortex. Analysis of single voxels and their discriminatory power showed that the representation of action in the stereo-action-specific areas was more accurate when stereopsis was active. Further analyses showed that the 4 stereo-action-specific sites form a closed network converging onto the premotor node, which connects to parietal and occipitotemporal regions outside the network. Several of the specific sites are known to process vestibular signals, suggesting that the network combines observed actions in peripersonal space with gravitational signals. These findings have wider implications for the function of premotor cortex and the role of stereopsis in human behavior. PMID:27252350

  13. Stereoscopically Observing Manipulative Actions

    PubMed Central

    Ferri, S.; Pauwels, K.; Rizzolatti, G.; Orban, G. A.

    2016-01-01

    The purpose of this study was to investigate the contribution of stereopsis to the processing of observed manipulative actions. To this end, we first combined the factors “stimulus type” (action, static control, and dynamic control), “stereopsis” (present, absent) and “viewpoint” (frontal, lateral) into a single design. Four sites in premotor, retro-insular (2) and parietal cortex operated specifically when actions were viewed stereoscopically and frontally. A second experiment clarified that the stereo-action-specific regions were driven by actions moving out of the frontoparallel plane, an effect amplified by frontal viewing in premotor cortex. Analysis of single voxels and their discriminatory power showed that the representation of action in the stereo-action-specific areas was more accurate when stereopsis was active. Further analyses showed that the 4 stereo-action-specific sites form a closed network converging onto the premotor node, which connects to parietal and occipitotemporal regions outside the network. Several of the specific sites are known to process vestibular signals, suggesting that the network combines observed actions in peripersonal space with gravitational signals. These findings have wider implications for the function of premotor cortex and the role of stereopsis in human behavior. PMID:27252350

  14. An investigation of sex-linked differences to the toxic and to the pharmacological actions of difenacoum: studies in mice and rats.

    PubMed

    Winn, M J; Clegg, J A; Park, B K

    1987-03-01

    We have investigated the actions of the coumarin anticoagulant, difenacoum, in male and female rats and mice. In our first experiment difenacoum (0.5 mg kg-1) killed 50% of male mice within 9 days of its administration, whereas no female mice died during this study. In a second group of experiments, the anticoagulant effect of difenacoum in male and female rats was determined. Under resting conditions, the prothrombin complex activities (PCA) of male and female rats were not significantly different. Over the first 24 h after administration of difenacoum (0.4 mg kg-1 i.p.), there was a monoexponential fall in PCA in both sexes. However, 6, 12 and 24 h after difenacoum, the PCA in male rats was significantly (P less than 0.05) lower than in female rats. PCA began to recover over the subsequent 48 h in both sexes, during which time there was marked variability in recovery in female rats. The difference between the onset of action of difenacoum in male and female rats did not appear to be due to a greater rate of elimination of the drug in female rats, since the plasma concentrations of difenacoum 24 h after its administration were the same in both sexes. The concentration of vitamin K1 in rat liver was also investigated. Vitamin K1 levels were 35.1 +/- 18.6 ng (g liver)-1 (male), and 29.4 +/- 5.4 ng (g liver)-1 (females) in control rats, but 24 h after difenacoum, vitamin K1 levels were either very low, or undetectable in all rats.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2883288

  15. Atherogenic, fibrotic and glucose utilising actions of glucokinase activators on vascular endothelium and smooth muscle

    PubMed Central

    2014-01-01

    Background Pharmaceutical interventions for diabetes aim to control glycaemia and to prevent the development of complications, such as cardiovascular diseases. Some anti-hyperglycaemic drugs have been found to have adverse cardiovascular effects in their own right, limiting their therapeutic role. Glucokinase activity in the pancreas is critical in enhancing insulin release in response to hyperglycaemia. Glucokinase activators (GKAs) are novel agents for diabetes which act by enhancing the formation of glucose-6-phosphate leading to increased insulin production and subsequent suppression of blood glucose. Little, however, is known about the direct effects of GKAs on cardiovascular cells. Methods The effect of the GKAs RO28-1675 and Compound A on glucose utilisation in bovine aortic endothelial cells (BAEC) and rat MIN6 was observed by culturing the cells at high and low glucose concentration in the presence and absence of the GKAs and measuring glucose consumption. The effect of RO28-1675 at various concentrations on glucose-dependent signalling in BAEC was observed by measuring Smad2 phosphorylation by Western blotting. The effect of RO28-1675 on TGF-β stimulated proteoglycan synthesis was measured by 35S-SO4 incorporation and assessment of proteoglycan size by SDS-PAGE. The effects of RO28-1675 on TGF-β mediated Smad2C phosphorylation in BAEC was observed by measurement of pSmad2C levels. The direct actions of RO28-1675 on vascular reactivity were observed by measuring arteriole tone and lumen diameter. Results GKAs were demonstrated to increase glucose utilisation in pancreatic but not endothelial cells. Glucose-activated Smad2 phosphorylation was decreased in a dose-dependent fashion in the presence of RO28-1675. No effect of RO28-1675 was observed on TGF-β stimulated proteoglycan production. RO28-1675 caused a modest dilation in arteriole but not contractile sensitivity. Conclusions GKA RO28-1675 did not increase glucose consumption in endothelial cells

  16. QM/MM Analysis of Cellulase Active Sites and Actions of the Enzymes on Substrates

    SciTech Connect

    Saharay, Moumita; Guo, Hao-Bo; Smith, Jeremy C; Guo, Hong

    2010-01-01

    Biodegradation of cellulosic biomass requires the actions of three types of secreted enzymes; endoglucanase (EC 3.2.1.4), cellobiohydrolase or exoglucanase (EC 3.2.1.91), and -glucosidase (EC 4.2.1.21). These enzymes act synergistically to hydrolyse the -1,4 bonds of cellulose and converts it into simple sugar. Hydrolysis of the glycosidic bond can occur either by net retention or by inversion of anomeric configuration at the anomeric center. QM/MM simulations are useful tools to study the energetics of the reactions and analyze the active-site structures at different states of the catalysis, including the formation of unstable transition states. Here, a brief description of previous work on glycoside hydrolases is first given. The results of the QM/MM potential energy and free energy simulations corresponding to glycosylation and deglycosylation processes are then provided for two retaining endoglucanases, Cel12A and Cel5A. The active-site structural features are analyzed based on the QM/MM results. The role of different residues and hydrogen bonding interactions during the catalysis and the importance of the sugar ring distortion are discussed for these two enzymes.

  17. Factors associated with regulatory action involving investigation of illnesses associated with Shiga toxin-producing Escherichia coli in products regulated by the Food Safety and Inspection Service.

    PubMed

    Green, Alice L; Seys, Scott; Douris, Aphrodite; Levine, Jeoff; Robertson, Kis

    2014-07-01

    We described characteristics of the Escherichia coli O157 and Escherichia coli non-O157 illness investigations conducted by the United States Department of Agriculture's Food Safety and Inspection Service (FSIS) during the 5-year period from 2006 through 2010. We created a multivariable logistic regression model to determine characteristics of these investigations that were associated with FSIS regulatory action, which was defined as having occurred if a product recall occurred or if FSIS personnel performed an environmental health assessment (Food Safety Assessment) at the implicated establishment. During this period, FSIS took regulatory action in 38 of 88 (43%) investigations. Illness investigations in which FoodNet states were involved were more likely to result in regulatory action. Illness investigations in which state and local traceback, or FSIS traceback occurred were more likely to result in regulatory action. Reasons for lack of action included evidence of cross-contamination after the product left a regulated establishment, delayed notification, lack of epidemiological information, and insufficient product information. PMID:24826872

  18. Antileishmanial activity and evaluation of the mechanism of action of strychnobiflavone flavonoid isolated from Strychnos pseudoquina against Leishmania infantum.

    PubMed

    Lage, Paula S; Chávez-Fumagalli, Miguel A; Mesquita, Juliana T; Mata, Laís M; Fernandes, Simone O A; Cardoso, Valbert N; Soto, Manuel; Tavares, Carlos A P; Leite, João P V; Tempone, Andre G; Coelho, Eduardo A F

    2015-12-01

    The present study aimed to investigate the in vitro antileishmanial activity of strychnobiflavone flavonoid against Leishmania infantum, as well as its mechanism of action, and evaluate the ex vivo biodistribution profile of the flavonoid in naive BALB/c mice. The antileishmanial activity (IC50 value) of strychnobiflavone against stationary promastigote and amastigote-like stages of the parasites was of 5.4 and 18.9 μM, respectively; with a 50% cytotoxic concentration (CC50) value of 125.0 μM on murine macrophages, resulting in selectivity index (SI) of 23.2 and 6.6, respectively. Amphotericin B, used as a positive control, presented SI values of 7.6 and 3.3 for promastigote and amastigote-like stages of L. infantum, respectively. The strychnobiflavone was also effective in reducing in significant levels the percentage of infected macrophages, as well as the number of amastigotes per macrophage, after the treatment of infected macrophages using the flavonoid. By using different fluorescent probes, we investigated the bioenergetics metabolism of L. infantum promastigotes and demonstrated that the flavonoid caused the depolarization of the mitochondrial membrane potential, without affecting the production of reactive oxygen species. In addition, using SYTOX(®) green as a fluorescent probe, the strychnobiflavone demonstrated no interference in plasma membrane permeability. For the ex vivo biodistribution assays, the flavonoid was labeled with technetium-(99m) and studied in a mouse model by intraperitoneal route. After a single dose administration, the scintigraphic images demonstrated a highest uptake by the liver and spleen of the animals within 60 min, resulting in low concentrations after 24 h. The present study therefore demonstrated, for the first time, the antileishmanial activity of the strychnobiflavone against L. infantum, and suggests that the mitochondria of the parasites may be the possible target organelle. The preferential distribution of this

  19. Antifungal Activity and Action Mechanism of Histatin 5-Halocidin Hybrid Peptides against Candida ssp.

    PubMed

    Han, Juhye; Jyoti, Md Anirban; Song, Ho-Yeon; Jang, Woong Sik

    2016-01-01

    The candidacidal activity of histatin 5 is initiated through cell wall binding, followed by translocation and intracellular targeting, while the halocidin peptide exerts its activity by attacking the Candida cell membrane. To improve antimicrobial activities and to understand the killing mechanism of two peptides, six hybrid peptides were designed by conjugating histatin 5 and halocidin. A comparative approach was established to study the activity, salt tolerance, cell wall glucan binding assay, cytotoxicity, generation of ROS and killing kinetics. CD spectrometry was conducted to evaluate secondary structures of these hybrid peptides. Furthermore the cellular localization of hybrid peptides was investigated by confocal fluorescence microscopy. Of the six hybrid congeners, di-PH2, di-WP2 and HHP1 had stronger activities than other hybrid peptides against all tested Candida strains. The MIC values of these peptides were 1-2, 2-4 and 2-4 μg/ml, respectively. Moreover, none of the hybrid peptides was cytotoxic in the hemolytic assay and cell-based cytotoxicity assay. Confocal laser microscopy showed that di-PH2 and HHP1 were translocated into cytoplasm whereas di-WP2 was accumulated on surface of C. albicans to exert their candidacidal activity. All translocated peptides (Hst 5, P113, di-PH2) were capable of generating intracellular ROS except HHP1. Additionally, the KFH residues at C-terminal end of these peptides were assumed for core sequence for active translocation.

  20. Antifungal Activity and Action Mechanism of Histatin 5-Halocidin Hybrid Peptides against Candida ssp

    PubMed Central

    Han, Juhye; Jyoti, Md. Anirban; Song, Ho-Yeon; Jang, Woong Sik

    2016-01-01

    The candidacidal activity of histatin 5 is initiated through cell wall binding, followed by translocation and intracellular targeting, while the halocidin peptide exerts its activity by attacking the Candida cell membrane. To improve antimicrobial activities and to understand the killing mechanism of two peptides, six hybrid peptides were designed by conjugating histatin 5 and halocidin. A comparative approach was established to study the activity, salt tolerance, cell wall glucan binding assay, cytotoxicity, generation of ROS and killing kinetics. CD spectrometry was conducted to evaluate secondary structures of these hybrid peptides. Furthermore the cellular localization of hybrid peptides was investigated by confocal fluorescence microscopy. Of the six hybrid congeners, di-PH2, di-WP2 and HHP1 had stronger activities than other hybrid peptides against all tested Candida strains. The MIC values of these peptides were 1–2, 2–4 and 2–4 μg/ml, respectively. Moreover, none of the hybrid peptides was cytotoxic in the hemolytic assay and cell-based cytotoxicity assay. Confocal laser microscopy showed that di-PH2 and HHP1 were translocated into cytoplasm whereas di-WP2 was accumulated on surface of C. albicans to exert their candidacidal activity. All translocated peptides (Hst 5, P113, di-PH2) were capable of generating intracellular ROS except HHP1. Additionally, the KFH residues at C-terminal end of these peptides were assumed for core sequence for active translocation. PMID:26918792

  1. Antifungal Activity and Action Mechanism of Histatin 5-Halocidin Hybrid Peptides against Candida ssp.

    PubMed

    Han, Juhye; Jyoti, Md Anirban; Song, Ho-Yeon; Jang, Woong Sik

    2016-01-01

    The candidacidal activity of histatin 5 is initiated through cell wall binding, followed by translocation and intracellular targeting, while the halocidin peptide exerts its activity by attacking the Candida cell membrane. To improve antimicrobial activities and to understand the killing mechanism of two peptides, six hybrid peptides were designed by conjugating histatin 5 and halocidin. A comparative approach was established to study the activity, salt tolerance, cell wall glucan binding assay, cytotoxicity, generation of ROS and killing kinetics. CD spectrometry was conducted to evaluate secondary structures of these hybrid peptides. Furthermore the cellular localization of hybrid peptides was investigated by confocal fluorescence microscopy. Of the six hybrid congeners, di-PH2, di-WP2 and HHP1 had stronger activities than other hybrid peptides against all tested Candida strains. The MIC values of these peptides were 1-2, 2-4 and 2-4 μg/ml, respectively. Moreover, none of the hybrid peptides was cytotoxic in the hemolytic assay and cell-based cytotoxicity assay. Confocal laser microscopy showed that di-PH2 and HHP1 were translocated into cytoplasm whereas di-WP2 was accumulated on surface of C. albicans to exert their candidacidal activity. All translocated peptides (Hst 5, P113, di-PH2) were capable of generating intracellular ROS except HHP1. Additionally, the KFH residues at C-terminal end of these peptides were assumed for core sequence for active translocation. PMID:26918792

  2. The revised EMA guideline for the investigation of bioequivalence for immediate release oral formulations with systemic action.

    PubMed

    Verbeeck, Roger K; Musuamba, Flora T

    2012-01-01

    On August 1, 2010, a revised guidance regarding bioequivalence (BE) assessment for the approval of innovator (bridging studies, variations, line extensions) and generic medicinal products in the EU came into effect (EMA Guideline on the Investigation of Bioequivalence, CPMP/EWP/QWP/1401/98 Rev. 1/Corr**, London, 20 January 2010). This guideline specifies the requirements for BE assessment for immediate release oral dosage forms with systemic action. Compared to the previous BE guideline of the EMA, clearer guidance is now given on several topics including BE assessment of highly variable drugs/drug products (HVDs/HVDPs), the use of metabolite data, acceptance criteria for narrow therapeutic index drugs (NTIDs), BCS-based biowaivers, and dose strength to be used in case of application for marketing authorization of several strengths. However, the health authorities of the various EU member states do not necessarily apply the same rules as far as substitution and switchability between medicinal products are concerned. Moreover, differences still exist between the BE guidelines of the major health authorities (FDA, EMA, NIHC, ...) on topics such as HVDs/HVDPs, NTIDs and BCS-based biowaivers. Global harmonization should be the next logical step to guarantee accessibility to safe and efficacious drug products for patients in all parts of the world. PMID:23148877

  3. Toward panoramic in situ mapping of action potential propagation in transgenic hearts to investigate initiation and therapeutic control of arrhythmias

    PubMed Central

    Dura, Miroslav; Schröder-Schetelig, Johannes; Luther, Stefan; Lehnart, Stephan E.

    2014-01-01

    To investigate the dynamics and propensity for arrhythmias in intact transgenic hearts comprehensively, optical strategies for panoramic fluorescence imaging of action potential (AP) propagation are essential. In particular, mechanism-oriented molecular studies usually depend on transgenic mouse hearts of only a few millimeters in size. Furthermore, the temporal scales of the mouse heart remain a challenge for panoramic fluorescence imaging with heart rates ranging from 200 min−1 (e.g., depressed sinus node function) to over 1200 min−1 during fast arrhythmias. To meet these challenging demands, we and others developed physiologically relevant mouse models and characterized their hearts with planar AP mapping. Here, we summarize the progress toward panoramic fluorescence imaging and its prospects for the mouse heart. In general, several high-resolution cameras are synchronized and geometrically arranged for panoramic voltage mapping and the surface and blood vessel anatomy documented through image segmentation and heart surface reconstruction. We expect that panoramic voltage imaging will lead to novel insights about molecular arrhythmia mechanisms through quantitative strategies and organ-representative analysis of intact mouse hearts. PMID:25249982

  4. 76 FR 35016 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Investigator...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    ... Collection; Comments Requested: Investigator Integrity Questionnaire ACTION: 30-Day Notice and request for... Form/Collection: Investigator Integrity Questionnaire. (3) Agency form number, if any, and the... questionnaire regarding the investigator's degree of professionalism. (5) An estimate of the total number...

  5. Investigation of molecular mechanisms in photodynamic action and radiobiology with nanosecond flash photolysis and pulse radiolysis. Progress report, 1982

    SciTech Connect

    Grossweiner, L.I.

    1983-01-01

    Liposomes have been employed as membrane models applicable to photosensitization in phototherapy procedures. The results with 8-methoxypsoralen, the sensitizer in PUVA therapy of psoriasis, show that singlet oxygen generated by near-uv (uv-A) irradiation induces membrane damage leading to lysis. A similar role of singlet oxygen has been shown for photosensitization of liposomes by methylene blue, with the new observation that hydrodynamic forces promote the lytic action initiated by singlet oxygen attack on an unsaturated site of phosphatidylcholine. Liposome photosensitization by hematoporphyrin follows a Type II mechanism mediated by singlet oxygen for low sensitizer concentrations, and a Type I, anoxic, mechanism when the hematoporphyrin is aggregated. Similar concentration effects obtain with hematoporphyrin derivative (hpd), the photosensitizer in photoradiation therapy of malignant tumors. Studies on the components of hpd separated by gel chromatography show that the putative biological active fraction can photosensitize membrane damage under oxic and anoxic conditions. The oxic pathway was suppressed by binding to human serum albumin, as involved in serum transport of hpd prior to localization in tumor tissue. A study on hematoporphyrin photosensitization of targets other than membranes has shown that singlet oxygen is responsible for the photosensitized inactivation of subtilisin BPN' and photooxidation of tryptophan in human and bovine serum albumin. In the case of the serum proteins, the singlet oxygen is generated by the sensitizer-protein complex and it may react with all protein in the system. 11 references.

  6. Quantitative structure-activity relationships of insecticides and plant growth regulators: comparative studies toward understanding the molecular mechanism of action.

    PubMed Central

    Iwamura, H; Nishimura, K; Fujita, T

    1985-01-01

    Emphasis was put on the comparative quantitative structure-activity approaches to the exploration of action mechanisms of structurally different classes of compounds showing the same type of activity as well as those of the same type of compounds having different actions. Examples were selected from studies performed on insecticides and plant growth regulators, i.e., neurotoxic carbamates, phosphates, pyrethroids and DDT analogs, insect juvenile hormone mimics, and cytokinin agonistic and antagonistic compounds. Similarities and dissimilarities in structures required to elicit activity between compounds classes were revealed in terms of physicochemical parameters, provoking further exploration and evoking insights into the molecular mechanisms of action which may lead to the development of new structures having better qualities. PMID:3905379

  7. Enhanced Neural Processing of Goal-directed Actions After Active Training in 4-Month-Old Infants.

    PubMed

    Bakker, Marta; Sommerville, Jessica A; Gredebäck, Gustaf

    2016-03-01

    The current study explores the neural correlates of action perception and its relation to infants' active experience performing goal-directed actions. Study 1 provided active training with sticky mittens that enables grasping and object manipulation in prereaching 4-month-olds. After training, EEG was recorded while infants observed images of hands grasping toward (congruent) or away from (incongruent) objects. We demonstrate that brief active training facilitates social perception as indexed by larger amplitude of the P400 ERP component to congruent compared with incongruent trials. Study 2 presented 4-month-old infants with passive training in which they observed an experimenter perform goal-directed reaching actions, followed by an identical ERP session to that used in Study 1. The second study did not demonstrate any differentiation between congruent and incongruent trials. These results suggest that (1) active experience alters the brains' response to goal-directed actions performed by others and (2) visual exposure alone is not sufficient in developing the neural networks subserving goal processing during action observation in infancy.

  8. Inhibitory control training for appetitive behaviour change: A meta-analytic investigation of mechanisms of action and moderators of effectiveness.

    PubMed

    Jones, Andrew; Di Lemma, Lisa C G; Robinson, Eric; Christiansen, Paul; Nolan, Sarah; Tudur-Smith, Catrin; Field, Matt

    2016-02-01

    Inhibitory control training (ICT) is a novel intervention in which participants learn to associate appetitive cues with inhibition of behaviour. We present a meta-analytic investigation of laboratory studies of ICT for appetitive behaviour change in which we investigate candidate mechanisms of action, individual differences that may moderate its effectiveness, and compare it to other psychological interventions. We conducted random-effects generic inverse variance meta-analysis on data from 14 articles (18 effect sizes in total). Participants who received ICT chose or consumed significantly less food or alcohol compared to control groups (SMD = 0.36, 95% CIs [0.24, 0.47]; Z = 6.18, p < .001; I(2) = 71%). Effect sizes were larger for motor (Go/No-Go and Stop Signal) compared to oculomotor (Antisaccade) ICT. The effects of ICT on behaviour were comparable to those produced by other psychological interventions, and effects of ICT on food intake were greater in participants who were attempting to restrict their food intake. The magnitude of the effect of ICT on behaviour was predicted by the proportion of successful inhibitions but was unrelated to the absolute number of trials in which appetitive cues were paired with the requirement to inhibit, or the contingency between appetitive cues and the requirement to inhibit. The effect of ICT on cue devaluation (primarily assessed with implicit association tests) was not statistically significant. Our analysis confirms the efficacy of ICT for short-term behaviour change in the laboratory, and we have demonstrated that its effectiveness may depend on pairings between appetitive cues and successful inhibition. We highlight the need for further research to translate these findings outside of the laboratory. PMID:26592707

  9. Antiplasmodial Activity and Mechanism of Action of RSM-932A, a Promising Synergistic Inhibitor of Plasmodium falciparum Choline Kinase

    PubMed Central

    Zimmerman, Tahl; Moneriz, Carlos; Diez, Amalia; Bautista, José Manuel; Gómez del Pulgar, Teresa; Cebrián, Arancha

    2013-01-01

    We have investigated the mechanism of action of inhibition of the choline kinase of P. falciparum (p.f.-ChoK) by two inhibitors of the human ChoKα, MN58b and RSM-932A, which have previously been shown to be potent antitumoral agents. The efficacy of these inhibitors against p.f.-ChoK is investigated using enzymatic and in vitro assays. While MN58b may enter the choline/phosphocholine binding site, RSM-932A appears to have an altogether novel mechanism of inhibition and is synergistic with respect to both choline and ATP. A model of inhibition for RSM-932A in which this inhibitor traps p.f.-ChoK in a phosphorylated intermediate state blocking phosphate transfer to choline is presented. Importantly, MN58b and RSM-932A have in vitro inhibitory activity in the low nanomolar range and are equally effective against chloroquine-sensitive and chloroquine-resistant strains. RSM-932A and MN58b significantly reduced parasitemia and induced the accumulation of trophozoites and schizonts, blocking intraerythrocytic development and interfering with parasite egress or invasion, suggesting a delay of the parasite maturation stage. The present data provide two new potent structures for the development of antimalarial compounds and validate p.f.-ChoK as an accessible drug target against the parasite. PMID:24041883

  10. Anti-human rhinovirus 2 activity and mode of action of quercetin-7-glucoside from Lagerstroemia speciosa.

    PubMed

    Song, Jae Hyoung; Park, Kwi Sung; Kwon, Dur Han; Choi, Hwa Jung

    2013-04-01

    Human rhinoviruses (HRVs) are a major cause of the common cold, but there is currently, no registered clinically effective antiviral chemotherapeutic agent for treatment of diseases caused by HRVs. In this study, we examined the antiviral activity of quercetin 7-glucoside (Q7G) from Lagerstroemia speciosa against human rhinovirus 2 (HRV2) using a cytopathic effect (CPE) reduction method. Furthermore, to elucidate the action of Q7G on HRV2 multiplication in more detail, we investigated the effect of Q7G on the infection cycle of HRV2 through time-of-addition study, reverse transcription-polymerase chain reaction analysis, and effects of Q7G on the infectivity of HRV2 particles. Q7G potently showed anti-HRV2 activity by reducing the formation of a visible CPE. Q7G also inhibited virus replication in the initial stage of virus infection by indirect interaction with virus particles, and ribavirin had a relative weaker efficacy compared to Q7G. Therefore, these data suggest that Q7G exerted its anti-HRV2 effect via the inhibition of virus replication in the early stage and these findings provide important information for the utilization of Q7G for HRV2 treatment.

  11. A Laboratory of Extremophiles: Iceland Coordination Action for Research Activities on Life in Extreme Environments (CAREX) Field Campaign.

    PubMed

    Marteinsson, Viggó; Vaishampayan, Parag; Kviderova, Jana; Mapelli, Francesca; Medori, Mauro; Calfapietra, Carlo; Aguilera, Angeles; Hamisch, Domenica; Reynisson, Eyjólfur; Magnússon, Sveinn; Marasco, Ramona; Borin, Sara; Calzada, Abigail; Souza-Egipsy, Virginia; González-Toril, Elena; Amils, Ricardo; Elster, Josef; Hänsch, Robert

    2013-02-25

    Existence of life in extreme environments has been known for a long time, and their habitants have been investigated by different scientific disciplines for decades. However, reports of multidisciplinary research are uncommon. In this paper, we report an interdisciplinary three-day field campaign conducted in the framework of the Coordination Action for Research Activities on Life in Extreme Environments (CAREX) FP7EU program, with participation of experts in the fields of life and earth sciences. In situ experiments and sampling were performed in a 20 m long hot springs system of different temperature (57 °C to 100 °C) and pH (2 to 4). Abiotic factors were measured to study their influence on the diversity. The CO2 and H2S concentration varied at different sampling locations in the system, but the SO2 remained the same. Four biofilms, mainly composed by four different algae and phototrophic protists, showed differences in photosynthetic activity. Varying temperature of the sampling location affects chlorophyll fluorescence, not only in the microbial mats, but plants (Juncus), indicating selective adaptation to the environmental conditions. Quantitative polymerase chain reaction (PCR), DNA microarray and denaturing gradient gel electrophoresis (DGGE)-based analysis in laboratory showed the presence of a diverse microbial population. Even a short duration (30 h) deployment of a micro colonizer in this hot spring system led to colonization of microorganisms based on ribosomal intergenic spacer (RISA) analysis. Polyphasic analysis of this hot spring system was possible due to the involvement of multidisciplinary approaches.

  12. Antioxidant activity and antiproliferative action of methanolic extract of Geum quellyon Sweet roots in human tumor cell lines.

    PubMed

    Russo, Alessandra; Cardile, Venera; Lombardo, Laura; Vanella, Luca; Vanella, Angelo; Garbarino, Juan Antonio

    2005-09-14

    Geum quellyon Sweet, a perennial herb of the Rosaceae family, has been used in the traditional medicine of the Mapuche Amerindians of Chile to treat tooth neuralgia, gastric inflammation, prostatitis and to regulate menstruation, and for its diuretic and aphrodisiac properties. Although many benefits have been claimed for this plant, few scientific studies are available in the literature. In this study, we investigated the antioxidant activity of a methanolic extract of Geum quellyon roots. We also examined the anticancer action of this plant on Caco-2 (colon adenocarcinoma cells), DU-145 (androgen-insensitive prostate cancer cells) and KB (oral squamous carcinoma cells) human tumor cell lines. Our data showed that Geum quellyon extract, containing tannins, exhibits interesting antioxidant properties, expressed by its capacity to scavenge 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH) and superoxide anion (O(2)*-), to inhibit xanthine oxidase activity, to chelate metals, and to protect plasmid DNA from cleavage induced by hydroxyl radicals (*OH) and nitric oxide (NO). These results may explain, at least in part, its use in Mapuche traditional medicine for gastric inflammation and prostatitis. The assays on human tumor cell lines demonstrated that this natural product exhibits a inhibitory effect on all human cancer cells examined, and seem to indicate that necrosis cell death is triggered in KB cells and Caco-2, while apoptotic cell demise appears to be induced in DU-145. The effect evidenced in Caco-2 cells can be in part correlated to a modulation of redox-sensitive mechanisms.

  13. Corrective action investigation plan for Corrective Action Unit 143: Area 25 contaminated waste dumps, Nevada Test Site, Nevada, Revision 1 (with Record of Technical Change No. 1 and 2)

    SciTech Connect

    USDOE Nevada Operations Office

    1999-06-28

    This plan contains the US Department of Energy, Nevada Operations Office's approach to collect the data necessary to evaluate correction action alternatives appropriate for the closure of Corrective Action Unit (CAU) 143 under the Federal Facility Agreement and Consent Order. Corrective Action Unit 143 consists of two waste dumps used for the disposal of solid radioactive wastes. Contaminated Waste Dump No.1 (CAS 25-23-09) was used for wastes generated at the Reactor Maintenance Assembly and Disassembly (R-MAD) Facility and Contaminated Waste Dump No.2 (CAS 25-23-03) was used for wastes generated at the Engine Maintenance Assembly and Disassembly (E-MAD) Facility. Both the R-MAD and E-MAD facilities are located in Area 25 of the Nevada Test Site. Based on site history, radionuclides are the primary constituent of concern and are located in these disposal areas; vertical and lateral migration of the radionuclides is unlikely; and if migration has occurred it will be limited to the soil beneath the Contaminated Waste Disposal Dumps. The proposed investigation will involve a combination of Cone Penetrometer Testing within and near the solid waste disposal dumps, field analysis for radionuclides and volatile organic compounds, as well as sample collection from the waste dumps and surrounding areas for off-site chemical, radiological, and geotechnical analyses. The results of this field investigation will support a defensible evaluation of corrective action alternatives in the corrective action decision document.

  14. Investigate the Child's Scientific Activities on Practical Child's Activity Books for the Kindergarten's Children

    ERIC Educational Resources Information Center

    Aldarabah, Intisar Turki; Al-Mouhtadi, Reham

    2015-01-01

    This research aimed to investigate the extent to which the interactive international curriculum is included in the "Child's Scientific Activities" issued by the Ministry of Education in Jordan, for the kindergarten stage according to the global criterion (NRC). In order to answer the study questions, an instrument was developed to…