Purification and Assay of Rubisco Activase from Leaves 1
Robinson, Simon P.; Streusand, Virginia J.; Chatfield, J. Mark; Portis, Archie R.
1988-01-01
Ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) activase protein was purified from spinach leaves by ammonium sulfate precipitation and ion exchange fast protein liquid chromatography. This resulted in 48-fold purification with 70% recovery of activity and yielded up to 18 milligrams of rubisco activase protein from 100 grams of leaves. Based on these figures, the protein comprised approximately 2% by weight of soluble protein in spinach (Spinacia oleracea L.) leaves. The preparations were at least 95% pure and were stable when frozen in liquid nitrogen. Addition of ATP during purification and storage was necessary to maintain activity. Assay of rubisco activase was based on its ability to promote activation of rubisco in the presence of ribulose-1,5-bisphosphate. There was an absolute requirement for ATP which could not be replaced by other nucleoside phosphates. The initial rate of increase of rubisco activity and the final rubisco specific activity achieved were both dependent on the concentration of rubisco activase. The initial rate was directly proportional to the rubisco activase concentration and was used as the basis of activity. The rate of activation of rubisco was also dependent on the rubisco concentration, suggesting that the activation process is a second order reaction dependent on the concentrations of both rubisco and rubisco activase. It is suggested that deactivation of rubisco occurs simultaneously with rubisco activase-mediated activation, and that rubisco activation state represents a dynamic equilibrium between these two processes. Images Fig. 2 PMID:16666412
Keown, Jeremy R; Griffin, Michael D W; Mertens, Haydyn D T; Pearce, F Grant
2013-07-12
Ribulose-bisphosphate carboxylase/oxygenase (Rubisco) activase uses the energy from ATP hydrolysis to remove tight binding inhibitors from Rubisco, thus playing a key role in regulating photosynthesis in plants. Although several structures have recently added much needed structural information for different Rubisco activase enzymes, the arrangement of these subunits in solution remains unclear. In this study, we use a variety of techniques to show that Rubisco activase forms a wide range of structures in solution, ranging from monomers to much higher order species, and that the distribution of these species is highly dependent on protein concentration. The data support a model in which Rubisco activase forms an open spiraling structure rather than a closed hexameric structure. At protein concentrations of 1 μM, corresponding to the maximal activity of the enzyme, Rubisco activase has an oligomeric state of 2-4 subunits. We propose a model in which Rubisco activase requires at least 1 neighboring subunit for hydrolysis of ATP.
Keown, Jeremy R.; Griffin, Michael D. W.; Mertens, Haydyn D. T.; Pearce, F. Grant
2013-01-01
Ribulose-bisphosphate carboxylase/oxygenase (Rubisco) activase uses the energy from ATP hydrolysis to remove tight binding inhibitors from Rubisco, thus playing a key role in regulating photosynthesis in plants. Although several structures have recently added much needed structural information for different Rubisco activase enzymes, the arrangement of these subunits in solution remains unclear. In this study, we use a variety of techniques to show that Rubisco activase forms a wide range of structures in solution, ranging from monomers to much higher order species, and that the distribution of these species is highly dependent on protein concentration. The data support a model in which Rubisco activase forms an open spiraling structure rather than a closed hexameric structure. At protein concentrations of 1 μm, corresponding to the maximal activity of the enzyme, Rubisco activase has an oligomeric state of 2–4 subunits. We propose a model in which Rubisco activase requires at least 1 neighboring subunit for hydrolysis of ATP. PMID:23720775
Drought stress and carbon assimilation in a warming climate: Reversible and irreversible impacts.
Feller, Urs
2016-09-20
Global change is characterized by increased CO 2 concentration in the atmosphere, increasing average temperature and more frequent extreme events including drought periods, heat waves and flooding. Especially the impacts of drought and of elevated temperature on carbon assimilation are considered in this review. Effects of extreme events on the subcellular level as well as on the whole plant level may be reversible, partially reversible or irreversible. The photosynthetically active biomass depends on the number and the size of mature leaves and the photosynthetic activity in this biomass during stress and subsequent recovery phases. The total area of active leaves is determined by leaf expansion and senescence, while net photosynthesis per leaf area is primarily influenced by stomatal opening (stomatal conductance), mesophyll conductance, activity of the photosynthetic apparatus (light absorption and electron transport, activity of the Calvin cycle) and CO 2 release by decarboxylation reactions (photorespiration, dark respiration). Water status, stomatal opening and leaf temperature represent a "magic triangle" of three strongly interacting parameters. The response of stomata to altered environmental conditions is important for stomatal limitations. Rubisco protein is quite thermotolerant, but the enzyme becomes at elevated temperature more rapidly inactivated (decarbamylation, reversible effect) and must be reactivated by Rubisco activase (carbamylation of a lysine residue). Rubisco activase is present under two forms (encoded by separate genes or products of alternative splicing of the pre-mRNA from one gene) and is very thermosensitive. Rubisco activase was identified as a key protein for photosynthesis at elevated temperature (non-stomatal limitation). During a moderate heat stress Rubisco activase is reversibly inactivated, but during a more severe stress (higher temperature and/or longer exposure) the protein is irreversibly inactivated, insolubilized and finally degraded. On the level of the leaf, this loss of photosynthetic activity may still be reversible when new Rubisco activase is produced by protein synthesis. Rubisco activase as well as enzymes involved in the detoxification of reactive oxygen species or in osmoregulation are considered as important targets for breeding crop plants which are still productive under drought and/or at elevated leaf temperature in a changing climate. Copyright © 2016 Elsevier GmbH. All rights reserved.
The activity of Rubisco's molecular chaperone, Rubisco activase, in leaf extracts
USDA-ARS?s Scientific Manuscript database
Rubisco frequently undergoes unproductive interactions with its sugar-phosphate substrate that stabilize active sites in an inactive conformation. Restoring catalytic competence to these sites requires the “molecular chiropractic” activity of Rubisco activase (activase). To make the study of activas...
Activation of Rubisco by activase in leaf extracts
USDA-ARS?s Scientific Manuscript database
The activation of ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) is catalyzed by a chaperone called Rubisco activase. Rubisco activation is an early target of moderate heat stress, due largely to the acute sensitivity of Rubisco activase to thermal inactivation. In the past, assay...
Structural changes associated with the acute thermal instability of Rubisco activase
USDA-ARS?s Scientific Manuscript database
The inhibition of photosynthesis at moderately high temperatures has been linked to a decrease in Rubisco activation, thought to be a consequence of the thermal instability of Rubisco's chaperone, Rubisco activase. To determine the structural basis for inactivation of Rubisco activase, the effects o...
USDA-ARS?s Scientific Manuscript database
The rapid release of tight-binding inhibitors from dead-end Rubisco complexes requires the activity of Rubisco activase, an AAA+ ATPase that utilizes chemo-mechanical energy to catalyze the reactivation of Rubisco. Activase is thought to play a central role in coordinating the rate of CO2 fixation w...
Wachter, Rebekka M; Salvucci, Michael E; Carmo-Silva, A Elizabete; Barta, Csengele; Genkov, Todor; Spreitzer, Robert J
2013-11-01
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is prone to inactivation from non-productive binding of sugar-phosphates. Reactivation of Rubisco requires conformational remodeling by a specific chaperone, Rubisco activase. Rubisco activase from tobacco and other plants in the family Solanaceae is an inefficient activator of Rubisco from non-Solanaceae plants and from the green alga Chlamydomonas reinhardtii. To determine if the Rubisco small subunit plays a role in the interaction with Rubisco activase, a hybrid Rubisco (SSNT) composed of tobacco small subunits and Chlamydomonas large subunits was constructed. The SSNT hybrid, like other hybrid Rubiscos containing plant small subunits, supported photoautotrophic growth in Chlamydomonas, but growth in air was much slower than for cells containing wild-type Rubisco. The kinetic properties of the SSNT hybrid Rubisco were similar to the wild-type enzyme, indicating that the poor growth in air was probably caused by disruption of pyrenoid formation and the consequent impairment of the CO2concentrating mechanism. Recombinant Rubisco activase from Arabidopsis activated the SSNT hybrid Rubisco and hybrid Rubiscos containing spinach and Arabidopsis small subunits at rates similar to the rates with wild-type Rubisco. However, none of the hybrid Rubiscos was activated by tobacco Rubisco activase. That replacement of Chlamydomonas small subunits with plant small subunits does not affect the species-specific interaction between Rubisco and Rubisco activase suggests that the association is not dominated by the small subunits that surround the Rubisco central solvent channel. Therefore, the geometry of a side-on binding mode is more consistent with the data than a top-on or ring-stacking binding mode.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Salvucci, Michael
Research examined the thermal stability and propensity for aggregation of wild type and the C- and N-terminally modified forms of activase to determine if loss of activity under heat stress is dependent on protein aggregation. The results showed that 1) loss of activity at high temperature is independent of aggregation; 2) activase with both C- and N-terminal S-Tags are more susceptible to aggregation than wild type activase, 3) aggregation is highly dependent on the concentration of Mg2+ and 4) the ATP analog, ATPgammaS, protects against both thermal inactivation and aggregation.
Identification and characterization of multiple rubisco activases in chemoautotrophic bacteria
Tsai, Yi-Chin Candace; Lapina, Maria Claribel; Bhushan, Shashi; Mueller-Cajar, Oliver
2015-01-01
Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) is responsible for almost all biological CO2 assimilation, but forms inhibited complexes with its substrate ribulose-1,5-bisphosphate (RuBP) and other sugar phosphates. The distantly related AAA+ proteins rubisco activase and CbbX remodel inhibited rubisco complexes to effect inhibitor release in plants and α-proteobacteria, respectively. Here we characterize a third class of rubisco activase in the chemolithoautotroph Acidithiobacillus ferrooxidans. Two sets of isoforms of CbbQ and CbbO form hetero-oligomers that function as specific activases for two structurally diverse rubisco forms. Mutational analysis supports a model wherein the AAA+ protein CbbQ functions as motor and CbbO is a substrate adaptor that binds rubisco via a von Willebrand factor A domain. Understanding the mechanisms employed by nature to overcome rubisco's shortcomings will increase our toolbox for engineering photosynthetic carbon dioxide fixation. PMID:26567524
Salvucci, Michael E.; Crafts-Brandner, Steven J.
2004-01-01
Inhibition of net photosynthesis (Pn) by moderate heat stress has been attributed to an inability of Rubisco activase to maintain Rubisco in an active form. To examine this proposal, the temperature response of Pn, Rubisco activation, chlorophyll fluorescence, and the activities of Rubisco and Rubisco activase were examined in species from contrasting environments. The temperature optimum of Rubisco activation was 10°C higher in the creosote bush (Larrea tridentata) compared with the Antarctic hairgrass (Deschampsia antarctica), resembling the temperature response of Pn. Pn increased markedly with increasing internal CO2 concentration in Antarctic hairgrass and creosote bush plants subjected to moderate heat stress even under nonphotorespiratory conditions. Nonphotochemical quenching of chlorophyll fluorescence, the effective quantum yield of photochemical energy conversion (ΔF/Fm′) and the maximum yield of PSII (Fv/Fm) were more sensitive to temperature in Antarctic hairgrass and two other species endemic to cold regions (i.e. Lysipomia pumila and spinach [Spinacea oleracea]) compared with creosote bush and three species (i.e. jojoba [Simmondsia chinensis], tobacco [Nicotiana tabacum], and cotton [Gossypium hirsutum]) from warm regions. The temperature response of activity and the rate of catalytic inactivation of Rubisco from creosote bush and Antarctic hairgrass were similar, whereas the optimum for ATP hydrolysis and Rubisco activation by recombinant creosote bush, cotton, and tobacco activase was 8°C to 10°C higher than for Antarctic hairgrass and spinach activase. These results support a role for activase in limiting photosynthesis at high temperature. PMID:15084731
Rubisco Activase Activity Assays
USDA-ARS?s Scientific Manuscript database
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase functions as a mechano-chemical motor protein using the energy from ATP hydrolysis to contort the structure of its target protein, Rubisco. This action modulates the activation state of Rubisco by removing tightly-bound inhibitory s...
Rubisco activase and wheat productivity under heat stress conditions
USDA-ARS?s Scientific Manuscript database
Rubisco activase (RCA) constrains the photosynthetic potential of plants at high temperature (heat stress). We hypothesized that endogenous levels of RCA could serve as an important determinant of plant productivity under heat stress conditions. In this study, we investigated the possible relation...
Biophysical characterization of higher plant Rubisco activase
USDA-ARS?s Scientific Manuscript database
Rubisco activase (Rca) is a chaperone-like protein of the AAA+ family, which uses mechanochemical energy derived from ATP hydrolysis to release tightly bound inhibitors from the active site of the primary carbon fixing enzyme ribulose 1,5-bisphosphate oxygenase/carboxylase (Rubisco). Mechanistic and...
Rubisco, Rubisco activase, and global climate change.
Sage, Rowan F; Way, Danielle A; Kubien, David S
2008-01-01
Global warming and the rise in atmospheric CO(2) will increase the operating temperature of leaves in coming decades, often well above the thermal optimum for photosynthesis. Presently, there is controversy over the limiting processes controlling photosynthesis at elevated temperature. Leading models propose that the reduction in photosynthesis at elevated temperature is a function of either declining capacity of electron transport to regenerate RuBP, or reductions in the capacity of Rubisco activase to maintain Rubisco in an active configuration. Identifying which of these processes is the principal limitation at elevated temperature is complicated because each may be regulated in response to a limitation in the other. Biochemical and gas exchange assessments can disentangle these photosynthetic limitations; however, comprehensive assessments are often difficult and, for many species, virtually impossible. It is proposed that measurement of the initial slope of the CO(2) response of photosynthesis (the A/C(i) response) can be a useful means to screen for Rubisco activase limitations. This is because a reduction in the Rubisco activation state should be most apparent at low CO(2) when Rubisco capacity is generally limiting. In sweet potato, spinach, and tobacco, the initial slope of the A/C(i) response shows no evidence of activase limitations at high temperature, as the slope can be accurately modelled using the kinetic parameters of fully activated Rubisco. In black spruce (Picea mariana), a reduction in the initial slope above 30 degrees C cannot be explained by the known kinetics of fully activated Rubisco, indicating that activase may be limiting at high temperatures. Because black spruce is the dominant species in the boreal forest of North America, Rubisco activase may be an unusually important factor determining the response of the boreal biome to climate change.
Purification of Rubisco Activase from Leaves or after Expression in Escherichia coli.
USDA-ARS?s Scientific Manuscript database
Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco’s catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco...
Enhancing Rubisco activity at higher temperatures by re-engineering of Rubisco activase
USDA-ARS?s Scientific Manuscript database
This report discusses the possibility of increasing plant performance under moderate heat stress by improving the thermal stability of Rubisco activase. • The research is driven by the observation that photosynthesis is acutely sensitive to inhibition by moderate heat stress and that this inhibitio...
USDA-ARS?s Scientific Manuscript database
Rubisco’s catalytic chaperone, Rubisco activase (Rca), uses the energy from ATP hydrolysis to restore catalytic competence to Rubisco. In Arabidopsis, inhibition of Rca activity by ADP is fine-tuned by redox regulation of the a-isoform. To elucidate the mechanism for Rca regulation in species contai...
Structure of Arabidopsis thaliana Rubisco activase.
Hasse, Dirk; Larsson, Anna M; Andersson, Inger
2015-04-01
The CO2-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is inactivated by the formation of dead-end complexes with inhibitory sugar phosphates. In plants and green algae, the ATP-dependent motor protein Rubisco activase restores catalytic competence by facilitating conformational changes in Rubisco that promote the release of the inhibitory compounds from the active site. Here, the crystal structure of Rubisco activase from Arabidopsis thaliana is presented at 2.9 Å resolution. The structure reveals an AAA+ two-domain structure. More than 100 residues in the protein were not visible in the electron-density map owing to conformational disorder, but were verified to be present in the crystal by mass spectrometry. Two sulfate ions were found in the structure. One was bound in the loop formed by the Walker A motif at the interface of the domains. A second sulfate ion was bound at the N-terminal end of the first helix of the C-terminal domain. The protein packs in a helical fashion in the crystal, as observed previously for Rubisco activase, but differences in the helical pitch indicate flexibility in the packing of the protein.
Hozain, Moh'd I; Salvucci, Michael E; Fokar, Mohamed; Holaday, A Scott
2010-01-01
Significant inhibition of photosynthesis occurs at temperatures only a few degrees (
USDA-ARS?s Scientific Manuscript database
Rubisco activase (RCA) is essential for the activation of Rubisco, the carboxylating enzyme of photosynthesis. In Arabidopsis, RCA is encoded by a single gene (At2g39730) that is alternatively spliced to form a large alpha-RCA and small beta-RCA isoform. The activity of Rubisco is controlled in res...
Jurczyk, Barbara; Pociecha, Ewa; Grzesiak, Maciej; Kalita, Katarzyna; Rapacz, Marcin
2016-07-01
Alternative splicing of the Rubisco activase gene was shown to be a point for optimization of photosynthetic carbon assimilation. It can be expected to be a stress-regulated event that depends on plant freezing tolerance. The aim of the study was to examine the relationships among Rubisco activity, the expression of two Rubisco activase splicing variants and photoacclimation to low temperature. The experiment was performed on two Lolium perenne genotypes with contrasting levels of freezing tolerance. The study investigated the effect of pre-hardening (15°C) and cold acclimation (4°C) on net photosynthesis, photosystem II photochemical activity, Rubisco activity and the expression of two splicing variants of the Rubisco activase gene. The results showed an induction of Rubisco activity at both 15°C and 4°C only in a highly freezing-tolerant genotype. The enhanced Rubisco activity after pre-hardening corresponded to increased expression of the splicing variant representing the large isoform, while the increase in Rubisco activity during cold acclimation was due to the activation of both transcript variants. These boosts in Rubisco activity also corresponded to an activation of non-photochemical mechanism of photoacclimation induced at low temperature exclusively in the highly freezing-tolerant genotype. In conclusion, enhanced expression of Rubisco activase splicing variants caused an increase in Rubisco activity during pre-hardening and cold acclimation in the more freezing-tolerant Lolium perenne genotype. The induction of the transcript variant representing the large isoform may be an important element of increasing the carbon assimilation rate supporting the photochemical mechanism of photosynthetic acclimation to cold. Copyright © 2016 Elsevier GmbH. All rights reserved.
Henderson, J Nathan; Kuriata, Agnieszka M; Fromme, Raimund; Salvucci, Michael E; Wachter, Rebekka M
2011-10-14
The rapid release of tight-binding inhibitors from dead-end ribulose-bisphosphate carboxylase/oxygenase (Rubisco) complexes requires the activity of Rubisco activase, an AAA+ ATPase that utilizes chemo-mechanical energy to catalyze the reactivation of Rubisco. Activase is thought to play a central role in coordinating the rate of CO(2) fixation with the light reactions of photosynthesis. Here, we present a 1.9 Å crystal structure of the C-domain core of creosote activase. The fold consists of a canonical four-helix bundle, from which a paddle-like extension protrudes that entails a nine-turn helix lined by an irregularly structured peptide strand. The residues Lys-313 and Val-316 involved in the species-specific recognition of Rubisco are located near the tip of the paddle. An ionic bond between Lys-313 and Glu-309 appears to stabilize the glycine-rich end of the helix. Structural superpositions onto the distant homolog FtsH imply that the paddles extend away from the hexameric toroid in a fan-like fashion, such that the hydrophobic sides of each blade bearing Trp-302 are facing inward and the polar sides bearing Lys-313 and Val-316 are facing outward. Therefore, we speculate that upon binding, the activase paddles embrace the Rubisco cylinder by placing their hydrophobic patches near the partner protein. This model suggests that conformational adjustments at the remote end of the paddle may relate to selectivity in recognition, rather than specific ionic contacts involving Lys-313. Additionally, the superpositions predict that the catalytically critical Arg-293 does not interact with the bound nucleotide. Hypothetical ring-ring stacking and peptide threading models for Rubisco reactivation are briefly discussed.
Leitao, Louis; Maoret, Jean-José; Biolley, Jean-Philippe
2007-01-01
We quantified the ozone impact on levels of Zea mays L. cv. Chambord mRNAs encoding C4-phosphoenolpyruvate carboxylase (C4-PEPc), ribulose-l,5-bisphosphate carboxylase/oxygenase small and large subunits (Rubisco-SSU and Rubisco-LSU, respectively) and Rubisco activase (RCA) using real-time RT-PCR. Foliar pigment content, PEPc and Rubisco protein amounts were simultaneously determined. Two experiments were performed to study the ozone response of the 5th and the 10th leaf. For each experiment, three ozone concentrations were tested in open-top chambers: non-filtered air (NF, control) and non-filtered air containing 40 (+40) and 80 nL L-1 (+80) ozone. Regarding the 5th leaf, +40 atmosphere induced a loss in pigmentation, PEPc and Rubisco activase mRNAs. However, it was unable to notably depress carboxylase protein amounts and mRNAs encoding Rubisco. Except for Rubisco mRNAs, all other measured parameters from 5th leaf were depressed by +80 atmosphere. Regarding the 10th leaf, +40 atmosphere increased photosynthetic pigments and transcripts encoding Rubisco and Rubisco activase. Rubisco and PEPc protein amounts were not drastically changed, even if they tended to be increased. Level of C4-PEPc mRNA remained almost stable. In response to +80 atmosphere, pigments and transcripts encoding PEPc were notably decreased. Rubisco and PEPc protein amounts also declined to a lesser extent. Conversely, the level of transcripts encoding both Rubisco subunits and Rubisco activase that were not consistently disturbed tended to be slightly augmented. So, the present study suggests that maize leaves can respond differentially to a similar ozone stress.
Khairy, Alaaldin Idris H; Oh, Mi Jeong; Lee, Seung Min; Kim, Da Som; Roh, Kwang Soo
2016-06-01
Toxic heavy metals such as cadmium (Cd) and copper (Cu) are global problems that are a growing threat to the environment. Despite some heavy metals are required for plant growth and development, others are considered toxic elements and do not play any known physiological role in plant cells. Elevated doses of Cd or Cu cause toxicity in plants and generate damages due to the stress condition and eventually cause a significant reduction in quantity and quality of crop plants. The nitric oxide (NO) donor sodium nitroprusside (SNP) is reported to alleviate the toxicity of some heavy metals like Cd and Cu. In the current study, the role of NO in alleviating stresses of Cd and Cu was investigated in in vitro -grown tobacco ( Nicotiana tabacum ) Based on plant growth, total chlorophyll contents, contents and activities of rubisco and rubisco activase. According to the results of this study, the growth and total chlorophyll contents of Cd/Cu stressed plants were hugely decreased in the absence of SNP, while the supplementation of SNP resulted in a significant increase of both fresh weight and total chlorophyll contents. Remarkable reductions of Rubisco and rubisco activase contents and activities were observed in Cd and Cu-induced plants. SNP supplementation showed the highest contents and activities of rubisco and rubisco activase compared to the control and Cu/Cd-stressed plants. Taken together, our findings suggest that SNP could play a protective role in regulation of plant responses to abiotic stresses such as Cd and Cu by enhancing Rubisco and Rubisco activase.
Lee, So Young; Damodaran, Puthanveettil Narayanankutty; Roh, Kwang Soo
2014-01-01
The present study was designed to evaluate the influence of salicylic acid (SA) on the growth of salt stress (sodium chloride) induced in tobacco plants. In addition, quantification of rubisco and rubisco activase contents of the plants was also determined in treatments with the control, 10−4 mM SA, 50 mM NaCl, 100 mM NaCl, 150 mM NaCl, SA + 50 mM NaCl, SA + 100 mM NaCl and SA + 150 mM NaCl, respectively after in vitro culture for 5 weeks. The growth of the tobacco plant decreased in 50 mM and 100 mM NaCl when not treated with SA. However, the growth was accelerated by SA, and the growth retardation caused by NaCl was improved by SA. The content of rubisco was improved by SA only in plants treated with 50 mM NaCl, and the activity of rubisco was increased by SA resulting in the decreased effect of NaCl, but only in 50 mM NaCl treated plants. The content of rubisco activase decreased due to NaCl, and SA did not improve the effect caused by NaCl. The activity of rubisco activase was increased by SA resulting in decreased activity caused by NaCl, but increased effect by SA was not recovered to the level of NaCl untreated plants. The activity of rubisco and rubisco activase, which decreased due to denaturing agents, did not demonstrate significant improvement when compared to the control. PMID:25313276
Mazar, Andrew P.; Koenig, Kathy; Kurdowska, Anna K.; Idell, Steven
2009-01-01
The proenzyme single-chain urokinase plasminogen activator (scuPA) more effectively resolved intrapleural loculations in rabbits with tetracycline (TCN)-induced loculation than a range of clinical doses of two-chain uPA (Abbokinase) and demonstrated a trend toward greater efficacy than single-chain tPA (Activase) (Idell S et al., Exp Lung Res 33: 419, 2007.). scuPA more slowly generates durable intrapleural fibrinolytic activity than Abbokinase or Activase, but the interactions of these agents with inhibitors in pleural fluids (PFs) have been poorly understood. PFs from rabbits with TCN-induced pleural injury treated with intrapleural scuPA, its inactive Ser195Ala mutant, Abbokinase, Activase, or vehicle, were analyzed to define the mechanism by which scuPA induces durable fibrinolysis. uPA activity was elevated in PFs of animals treated with scuPA, correlated with the ability to clear pleural loculations, and resisted (70–80%) inhibition by PAI-1. α-macroglobulin (αM) but not urokinase receptor complexes immunoprecipitated from PFs of scuPA-treated rabbits retained uPA activity that resists PAI-1 and activates plasminogen. Conversely, little plasminogen activating or enzymatic activity resistant to PAI-1 was detectable in PFs of rabbits treated with Abbokinase or Activase. Consistent with these findings, PAI-1 interacts with scuPA much slower than with Activase or Abbokinase in vitro. An equilibrium between active and inactive scuPA (kon = 4.3 h−1) limits the rate of its inactivation by PAI-1, favoring formation of complexes with αM. These observations define a newly recognized mechanism that promotes durable intrapleural fibrinolysis via formation of αM/uPA complexes. These complexes promote uPA-mediated plasminogen activation in scuPA-treated rabbits with TCN-induced pleural injury. PMID:19666776
Phillips, Susie B; Batlivala, Sarosh; Knudson, Jarrod D
2015-10-01
Common aetiologies of sudden cardiac death in children include coronary anomalies, channelopathies, and cardiomyopathies. Less frequently, hypercoagulable states cause sudden arrest. We report an unusual case of aborted sudden cardiac death in a teenager, ultimately found to have homozygosity for the 4G allele of the plasminogen activase inhibitor type 1 gene.
Engineering Rubisco activase from thermophilic cyanobacteria into high-temperature sensitive plants.
Ogbaga, Chukwuma C; Stepien, Piotr; Athar, Habib-Ur-Rehman; Ashraf, Muhammad
2018-06-01
In the past decade, various strategies to improve photosynthesis and crop yield, such as leaf morphology, light interception and use efficiency, biochemistry of light reactions, stomatal conductance, carboxylation efficiency, and source to sink regulation, have been discussed at length. Leaf morphology and physiology are tightly coupled to light capturing efficiency, gas exchange capacity, and temperature regulation. However, apart from the photoprotective mechanism of photosystem-II (PSII), i.e. non-photochemical quenching, very low genetic variation in the components of light reactions has been observed in plants. In the last decade, biochemistry-based enhancement of carboxylation efficiency that improves photosynthesis in plants was one of the potential strategies for improving plant biomass production. Enhancement of activation of the ubiquitous enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) by Rubisco activase may be another potential strategy for improving a photosynthesis-driven increase in crop yield. Rubisco activase modifies the conformation of the active center in Rubisco by removing tightly bound inhibitors, thereby contributing to enzyme activation and rapid carboxylation. Thermophilic cyanobacteria are oxygenic photosynthetic bacteria that thrive in high-temperature environments. This critical review discusses the prospects for and the potential of engineering Rubisco activase from thermophilic cyanobacteria into temperature-sensitive plants, to increase the threshold temperature and survival of these plants in arid regions.
Characterization of the heterooligomeric red-type rubisco activase from red algae
Loganathan, Nitin; Tsai, Yi-Chin Candace; Mueller-Cajar, Oliver
2016-01-01
The photosynthetic CO2-fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) is inhibited by nonproductive binding of its substrate ribulose-1,5-bisphosphate (RuBP) and other sugar phosphates. Reactivation requires ATP-hydrolysis–powered remodeling of the inhibited complexes by diverse molecular chaperones known as rubisco activases (Rcas). Eukaryotic phytoplankton of the red plastid lineage contain so-called red-type rubiscos, some of which have been shown to possess superior kinetic properties to green-type rubiscos found in higher plants. These organisms are known to encode multiple homologs of CbbX, the α-proteobacterial red-type activase. Here we show that the gene products of two cbbX genes encoded by the nuclear and plastid genomes of the red algae Cyanidioschyzon merolae are nonfunctional in isolation, but together form a thermostable heterooligomeric Rca that can use both α-proteobacterial and red algal-inhibited rubisco complexes as a substrate. The mechanism of rubisco activation appears conserved between the bacterial and the algal systems and involves threading of the rubisco large subunit C terminus. Whereas binding of the allosteric regulator RuBP induces oligomeric transitions to the bacterial activase, it merely enhances the kinetics of ATP hydrolysis in the algal enzyme. Mutational analysis of nuclear and plastid isoforms demonstrates strong coordination between the subunits and implicates the nuclear-encoded subunit as being functionally dominant. The plastid-encoded subunit may be catalytically inert. Efforts to enhance crop photosynthesis by transplanting red algal rubiscos with enhanced kinetics will need to take into account the requirement for a compatible Rca. PMID:27872295
Characterization of the heterooligomeric red-type rubisco activase from red algae.
Loganathan, Nitin; Tsai, Yi-Chin Candace; Mueller-Cajar, Oliver
2016-12-06
The photosynthetic CO 2 -fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) is inhibited by nonproductive binding of its substrate ribulose-1,5-bisphosphate (RuBP) and other sugar phosphates. Reactivation requires ATP-hydrolysis-powered remodeling of the inhibited complexes by diverse molecular chaperones known as rubisco activases (Rcas). Eukaryotic phytoplankton of the red plastid lineage contain so-called red-type rubiscos, some of which have been shown to possess superior kinetic properties to green-type rubiscos found in higher plants. These organisms are known to encode multiple homologs of CbbX, the α-proteobacterial red-type activase. Here we show that the gene products of two cbbX genes encoded by the nuclear and plastid genomes of the red algae Cyanidioschyzon merolae are nonfunctional in isolation, but together form a thermostable heterooligomeric Rca that can use both α-proteobacterial and red algal-inhibited rubisco complexes as a substrate. The mechanism of rubisco activation appears conserved between the bacterial and the algal systems and involves threading of the rubisco large subunit C terminus. Whereas binding of the allosteric regulator RuBP induces oligomeric transitions to the bacterial activase, it merely enhances the kinetics of ATP hydrolysis in the algal enzyme. Mutational analysis of nuclear and plastid isoforms demonstrates strong coordination between the subunits and implicates the nuclear-encoded subunit as being functionally dominant. The plastid-encoded subunit may be catalytically inert. Efforts to enhance crop photosynthesis by transplanting red algal rubiscos with enhanced kinetics will need to take into account the requirement for a compatible Rca.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Liu Chao; Hong Fashui; Wu Kang
Neodymium (Nd), as a member of rare earth elements, proved to enhance the photosynthesis rate and organic substance accumulation of spinach through the increase in carboxylation activity of Rubisco. Although the oxygenase activity of spinach Rubisco was slightly changed with the Nd{sup 3+} treatment, the specific factor of Rubisco was greatly increased. It was partially due to the promotion of Rubisco activase (R-A) activity but mainly to the formation of Rubisco-Rubisco activase super-complex, a heavier molecular mass protein (about 1200 kD) comprising both Rubisco and Rubisco activase. This super-complex was found during the extraction procedure of Rubisco by the gelmore » electrophoresis and Western-blot studies. The formation of Rubisco-R-A super-complex suggested that the secondary structure of the protein purified from the Nd{sup 3+}-treated spinach was different from that of the control. Extended X-ray absorption fine structure study of the 'Rubisco' purified from the Nd{sup 3+}-treated spinach revealed that Nd was bound with four oxygen atoms and two sulfur atoms of amino acid residues at the Nd-O and Nd-S bond lengths of 2.46 and 2.89 A, respectively.« less
Evolution of Rubisco activase gene in plants.
Nagarajan, Ragupathi; Gill, Kulvinder S
2018-01-01
Rubisco activase of plants evolved in a stepwise manner without losing its function to adapt to the major evolutionary events including endosymbiosis and land colonization. Rubisco activase is an essential enzyme for photosynthesis, which removes inhibitory sugar phosphates from the active sites of Rubisco, a process necessary for Rubisco activation and carbon fixation. The gene probably evolved in cyanobacteria as different species differ for its presence. However, the gene is present in all other plant species. At least a single gene copy was maintained throughout plant evolution; but various genome and gene duplication events, which occurred during plant evolution, increased its copy number in some species. The exons and exon-intron junctions of present day higher plant's Rca, which is conserved in most species seem to have evolved in charophytes. A unique tandem duplication of Rca gene occurred in a common grass ancestor, and the two genes evolved differently for gene structure, sequence, and expression pattern. At the protein level, starting with a primitive form in cyanobacteria, RCA of chlorophytes evolved by integrating chloroplast transit peptide (cTP), and N-terminal domains to the ATPase, Rubisco recognition and C-terminal domains. The redox regulated C-terminal extension (CTE) and the associated alternate splicing mechanism, which splices the RCA-α and RCA-β isoforms were probably gained from another gene in charophytes, conserved in most species except the members of Solanaceae family.
Rubisco Activases: AAA+ Chaperones Adapted to Enzyme Repair.
Bhat, Javaid Y; Thieulin-Pardo, Gabriel; Hartl, F Ulrich; Hayer-Hartl, Manajit
2017-01-01
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the key enzyme of the Calvin-Benson-Bassham cycle of photosynthesis, requires conformational repair by Rubisco activase for efficient function. Rubisco mediates the fixation of atmospheric CO 2 by catalyzing the carboxylation of the five-carbon sugar ribulose-1,5-bisphosphate (RuBP). It is a remarkably inefficient enzyme, and efforts to increase crop yields by bioengineering Rubisco remain unsuccessful. This is due in part to the complex cellular machinery required for Rubisco biogenesis and metabolic maintenance. To function, Rubisco must undergo an activation process that involves carboxylation of an active site lysine by a non-substrate CO 2 molecule and binding of a Mg 2+ ion. Premature binding of the substrate RuBP results in an inactive enzyme. Moreover, Rubisco can also be inhibited by a range of sugar phosphates, some of which are "misfire" products of its multistep catalytic reaction. The release of the inhibitory sugar molecule is mediated by the AAA+ protein Rubisco activase (Rca), which couples hydrolysis of ATP to the structural remodeling of Rubisco. Rca enzymes are found in the vast majority of photosynthetic organisms, from bacteria to higher plants. They share a canonical AAA+ domain architecture and form six-membered ring complexes but are diverse in sequence and mechanism, suggesting their convergent evolution. In this review, we discuss recent advances in understanding the structure and function of this important group of client-specific AAA+ proteins.
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Rubisco Activases: AAA+ Chaperones Adapted to Enzyme Repair
Bhat, Javaid Y.; Thieulin-Pardo, Gabriel; Hartl, F. Ulrich; Hayer-Hartl, Manajit
2017-01-01
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the key enzyme of the Calvin-Benson-Bassham cycle of photosynthesis, requires conformational repair by Rubisco activase for efficient function. Rubisco mediates the fixation of atmospheric CO2 by catalyzing the carboxylation of the five-carbon sugar ribulose-1,5-bisphosphate (RuBP). It is a remarkably inefficient enzyme, and efforts to increase crop yields by bioengineering Rubisco remain unsuccessful. This is due in part to the complex cellular machinery required for Rubisco biogenesis and metabolic maintenance. To function, Rubisco must undergo an activation process that involves carboxylation of an active site lysine by a non-substrate CO2 molecule and binding of a Mg2+ ion. Premature binding of the substrate RuBP results in an inactive enzyme. Moreover, Rubisco can also be inhibited by a range of sugar phosphates, some of which are “misfire” products of its multistep catalytic reaction. The release of the inhibitory sugar molecule is mediated by the AAA+ protein Rubisco activase (Rca), which couples hydrolysis of ATP to the structural remodeling of Rubisco. Rca enzymes are found in the vast majority of photosynthetic organisms, from bacteria to higher plants. They share a canonical AAA+ domain architecture and form six-membered ring complexes but are diverse in sequence and mechanism, suggesting their convergent evolution. In this review, we discuss recent advances in understanding the structure and function of this important group of client-specific AAA+ proteins. PMID:28443288
USDA-ARS?s Scientific Manuscript database
A methodology is presented to characterize complex protein assembly pathways by fluorescence correlation spectroscopy. We have derived the total autocorrelation function describing the behavior of mixtures of labeled and unlabeled protein under equilibrium conditions. Our modeling approach allows us...
Mechanism of Enzyme Repair by the AAA+ Chaperone Rubisco Activase.
Bhat, Javaid Y; Miličić, Goran; Thieulin-Pardo, Gabriel; Bracher, Andreas; Maxwell, Andrew; Ciniawsky, Susanne; Mueller-Cajar, Oliver; Engen, John R; Hartl, F Ulrich; Wendler, Petra; Hayer-Hartl, Manajit
2017-09-07
How AAA+ chaperones conformationally remodel specific target proteins in an ATP-dependent manner is not well understood. Here, we investigated the mechanism of the AAA+ protein Rubisco activase (Rca) in metabolic repair of the photosynthetic enzyme Rubisco, a complex of eight large (RbcL) and eight small (RbcS) subunits containing eight catalytic sites. Rubisco is prone to inhibition by tight-binding sugar phosphates, whose removal is catalyzed by Rca. We engineered a stable Rca hexamer ring and analyzed its functional interaction with Rubisco. Hydrogen/deuterium exchange and chemical crosslinking showed that Rca structurally destabilizes elements of the Rubisco active site with remarkable selectivity. Cryo-electron microscopy revealed that Rca docks onto Rubisco over one active site at a time, positioning the C-terminal strand of RbcL, which stabilizes the catalytic center, for access to the Rca hexamer pore. The pulling force of Rca is fine-tuned to avoid global destabilization and allow for precise enzyme repair. Copyright © 2017 Elsevier Inc. All rights reserved.
Structural Changes Associated with the Acute Thermal Instability of Rubisco Activase
USDA-ARS?s Scientific Manuscript database
The inhibition of photosynthesis at moderately high temperatures has been linked to a decrease in ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activation. This decrease is thought to be a consequence of the thermal instability of Rubisco’s chaperone, ribulose-1,5-bisphosphate carboxyla...
Molecular architectures and functions of radical enzymes and their (re)activating proteins.
Shibata, Naoki; Toraya, Tetsuo
2015-10-01
Certain proteins utilize the high reactivity of radicals for catalysing chemically challenging reactions. These proteins contain or form a radical and therefore named 'radical enzymes'. Radicals are introduced by enzymes themselves or by (re)activating proteins called (re)activases. The X-ray structures of radical enzymes and their (re)activases revealed some structural features of these molecular apparatuses which solved common enigmas of radical enzymes—i.e. how the enzymes form or introduce radicals at the active sites, how they use the high reactivity of radicals for catalysis, how they suppress undesired side reactions of highly reactive radicals and how they are (re)activated when inactivated by extinction of radicals. This review highlights molecular architectures of radical B12 enzymes, radical SAM enzymes, tyrosyl radical enzymes, glycyl radical enzymes and their (re)activating proteins that support their functions. For generalization, comparisons of the recently reported structures of radical enzymes with those of canonical radical enzymes are summarized here. © The Authors 2015. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
USDA-ARS?s Scientific Manuscript database
Protein elongation factors, EF-Tu and EF-1a, have been implicated in cell response to heat stress. In spring wheat, EF-Tu displays chaperone activity and reduces thermal aggregation of Rubisco activase. Similarly, in mammalian cells, EF-1a displays chaperone-like activity and regulates the expressio...
USDA-ARS?s Scientific Manuscript database
Significant inhibition of photosynthesis occurs at temperatures only a few degrees (less than or equal to 10 degrees Celsius) above the optimum, resulting in considerable loss of potential productivity. Most studies of heat stress have focused on crop or weedy annual plants, whereas similar studies...
Parry, Martin A. J.; Keys, Alfred J.; Foyer, Christine H.; Furbank, Robert T.; Walker, David A.
1988-01-01
Ribulose-1,5-bisphosphate (RuBP) carboxylase in lysed spinach (Spinacia oleracea L. cv virtuosa) chloroplasts that had been partly inactivated at low CO2 and Mg2+ by incubating in darkness with 4 millimolar partially purified RuBP was reactivated by light. If purified RuBP was used to inhibit dark activation of the enzyme, reactivation by light was not observed unless fructose-1,6-bisphosphate, ATP, or ADP plus inorganic phosphate were also added. Presumably, ADP plus inorganic phosphate acted as an ATP-generating system with a requirement for the generation of ΔpH across the thylakoid membrane. When the RuBP obtained from Sigma Chemical Co. was used, light did not reactivate the enzyme. There was no direct correlation between ΔpH and activation. Therefore, thylakoids are required in the ribulose-1,5-bisphosphate carboxylase activase system largely to synthesize ATP. Inactivation of RuBP carboxylase in isolated chloroplasts or in the lysed chloroplast system was not promoted simply by a transition from light to dark conditions but was caused by low CO2 and Mg2+. PMID:16666184
Blayney, Michelle J; Whitney, Spencer M; Beck, Jennifer L
2011-09-01
Ribulose bisphosphate carboxylase/oxygenase (Rubisco) is the protein that is responsible for the fixation of carbon dioxide in photosynthesis. Inhibitory sugar phosphate molecules, which can include its substrate ribulose-1,5-bisphosphate (RuBP), can bind to Rubisco catalytic sites and inhibit catalysis. These are removed by interaction with Rubisco activase (RA) via an ATP hydrolytic reaction. Here we show the first nanoESI mass spectra of the hexadecameric Rubisco and of RA from a higher plant (tobacco). The spectra of recombinant, purified RA revealed polydispersity in its oligomeric forms (up to hexamer) and that ADP was bound. ADP was removed by dialysis against a high ionic strength solution and nucleotide binding experiments showed that ADP bound more tightly to RA than AMP-PNP (a non-hydrolysable ATP analog). There was evidence that there may be two nucleotide binding sites per RA monomer. The oligomerization capacity of mutant and wild-type tobacco RA up to hexamers is analogous to the subunit stoichiometry for other AAA+ enzymes. This suggests assembly of RA into hexamers is likely the most active conformation for removing inhibitory sugar phosphate molecules from Rubisco to enable its catalytic competency. Stoichiometric binding of RuBP or carboxyarabinitol bisphosphate (CABP) to each of the eight catalytic sites of Rubisco was observed.
Scafaro, Andrew P; Gallé, Alexander; Van Rie, Jeroen; Carmo-Silva, Elizabete; Salvucci, Michael E; Atwell, Brian J
2016-08-01
The mechanistic basis of tolerance to heat stress was investigated in Oryza sativa and two wild rice species, Oryza meridionalis and Oryza australiensis. The wild relatives are endemic to the hot, arid Australian savannah. Leaf elongation rates and gas exchange were measured during short periods of supra-optimal heat, revealing species differences. The Rubisco activase (RCA) gene from each species was sequenced. Using expressed recombinant RCA and leaf-extracted RCA, the kinetic properties of the two isoforms were studied under high temperatures. Leaf elongation was undiminished at 45°C in O. australiensis. The net photosynthetic rate was almost 50% slower in O. sativa at 45°C than at 28°C, while in O. australiensis it was unaffected. Oryza meridionalis exhibited intermediate heat tolerance. Based on previous reports that RCA is heat-labile, the Rubisco activation state was measured. It correlated positively with leaf elongation rates across all three species and four periods of exposure to 45°C. Sequence analysis revealed numerous polymorphisms in the RCA amino acid sequence from O. australiensis. The O. australiensis RCA enzyme was thermally stable up to 42°C, contrasting with RCA from O. sativa, which was inhibited at 36°C. We attribute heat tolerance in the wild species to thermal stability of RCA, enabling Rubisco to remain active. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
Scales, Joanna C; Parry, Martin A J; Salvucci, Michael E
2014-03-01
Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes carboxylation of ribulose-1,5-bisphosphate, the first in a series of reactions leading to the incorporation of atmospheric CO₂ into biomass. Rubisco requires Rubisco activase (RCA), an AAA+ ATPase that reactivates Rubisco by remodelling the conformation of inhibitor-bound sites. RCA is regulated by the ratio of ADP:ATP, with the precise response potentiated by redox regulation of the alpha-isoform. Measuring the effects of ADP on the activation of Rubisco by RCA using the well-established photometric assay is problematic because of the adenine nucleotide requirement of 3-phosphoglycerate (3-PGA) kinase. Described here is a novel assay for measuring RCA activity in the presence of variable ratios of ADP:ATP. The assay couples the formation of 3-PGA from ribulose 1,5-bisphosphate and CO₂ to NADH oxidation through cofactor-dependent phosphoglycerate mutase, enolase, PEP carboxylase and malate dehydrogenase. The assay was used to determine the effects of Rubisco and RCA concentration and ADP:ATP ratio on RCA activity, and to measure the activation of a modified Rubisco by RCA. Variations of the basic assay were used to measure the activation state of Rubisco in leaf extracts and the activity of purified Rubisco. The assay can be automated for high-throughput processing by conducting the reactions in two stages.
NASA Astrophysics Data System (ADS)
Wang, Quan; Serban, Andrew J.; Wachter, Rebekka M.; Moerner, W. E.
2018-03-01
Oligomerization plays an important role in the function of many proteins, but a quantitative picture of the oligomer distribution has been difficult to obtain using existing techniques. Here we describe a method that combines sub-stoichiometric labeling and recently developed single-molecule diffusometry to measure the size distribution of oligomers under equilibrium conditions in solution, one molecule at a time. We use this technique to characterize the oligomerization behavior of Nicotiana tabacum (Nt) Rubisco activase (Nt-Rca), a chaperone-like AAA-plus ATPase essential in regulating carbon fixation during photosynthesis. We directly observed monomers, dimers, and a tetramer/hexamer mixture and extracted their fractional abundance as a function of protein concentration. We show that the oligomerization pathway of Nt-Rca is nucleotide dependent: ATPγS binding strongly promotes tetramer/hexamer formation from dimers and results in a preferred tetramer/hexamer population for concentrations in the 1-10 μM range. Furthermore, we directly observed dynamic assembly and disassembly processes of single complexes in real time and from there estimated the rate of subunit exchange to be ˜0.1 s-1 with ATPγS. On the other hand, ADP binding destabilizes Rca complexes by enhancing the rate of subunit exchange by >2 fold. These observations provide a quantitative starting point to elucidate the structure-function relations of Nt-Rca complexes. We envision the method to fill a critical gap in defining and quantifying protein assembly pathways in the small-oligomer regime.
Wang, Quan; Serban, Andrew J; Wachter, Rebekka M; Moerner, W E
2018-03-28
Oligomerization plays an important role in the function of many proteins, but a quantitative picture of the oligomer distribution has been difficult to obtain using existing techniques. Here we describe a method that combines sub-stoichiometric labeling and recently developed single-molecule diffusometry to measure the size distribution of oligomers under equilibrium conditions in solution, one molecule at a time. We use this technique to characterize the oligomerization behavior of Nicotiana tabacum (Nt) Rubisco activase (Nt-Rca), a chaperone-like AAA-plus ATPase essential in regulating carbon fixation during photosynthesis. We directly observed monomers, dimers, and a tetramer/hexamer mixture and extracted their fractional abundance as a function of protein concentration. We show that the oligomerization pathway of Nt-Rca is nucleotide dependent: ATPγS binding strongly promotes tetramer/hexamer formation from dimers and results in a preferred tetramer/hexamer population for concentrations in the 1-10 μM range. Furthermore, we directly observed dynamic assembly and disassembly processes of single complexes in real time and from there estimated the rate of subunit exchange to be ∼0.1 s -1 with ATPγS. On the other hand, ADP binding destabilizes Rca complexes by enhancing the rate of subunit exchange by >2 fold. These observations provide a quantitative starting point to elucidate the structure-function relations of Nt-Rca complexes. We envision the method to fill a critical gap in defining and quantifying protein assembly pathways in the small-oligomer regime.
Bi, Huangai; Liu, Peipei; Jiang, Zhensheng; Ai, Xizhen
2017-10-01
Rubisco activase (RCA) is an important enzyme that can catalyze the carboxylation and oxygenation activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which is involved in the photosynthetic carbon reduction cycle. Here, we studied the effects of changes in RCA activity on photosynthesis, growth and development, as well as the low temperature and weak light tolerance of RCA overexpressing transgenic cucumber (Cucumis sativus) plants. CsRCA overexpression increased the plant height, leaf area and dry matter, and decreased the root/top ratio in transgenic cucumber plants compared with the wild-type (WT) plants. Low temperature and low light stress led to decreases in the CsRCA expression and protein levels, the photosynthetic rate (Pn) and the stomatal conductance (Gs), but an increase in the intercellular CO 2 (Ci) concentration in cucumber leaves. The actual photochemical efficiency and maximal photochemical efficiency of photosystem II in cucumber seedlings also declined, but the initial fluorescence increased during low temperature and weak light stress. Transgenic plants showed a lower decrease in the CsRCA expression level and actual and maximal photochemical efficiencies, as well as increases in the Ci and initial fluorescence relative to the WT plants. Low temperature and low light stress resulted in a significant increase in the malondialdehyde (MDA) content; however, this increase was reduced in transgenic plants compared with that in WT plants. Thus, the overexpression of CsRCA may promote the growth and low temperature and low light tolerance of cucumber plants in solar greenhouses. © 2017 Scandinavian Plant Physiology Society.
Shivhare, Devendra; Mueller-Cajar, Oliver
2017-07-01
To maintain metabolic flux through the Calvin-Benson-Bassham cycle in higher plants, dead-end inhibited complexes of Rubisco must constantly be engaged and remodeled by the molecular chaperone Rubisco activase (Rca). In C3 plants, the thermolability of Rca is responsible for the deactivation of Rubisco and reduction of photosynthesis at moderately elevated temperatures. We reasoned that crassulacean acid metabolism (CAM) plants must possess thermostable Rca to support Calvin-Benson-Bassham cycle flux during the day when stomata are closed. A comparative biochemical characterization of rice ( Oryza sativa ) and Agave tequilana Rca isoforms demonstrated that the CAM Rca isoforms are approximately10°C more thermostable than the C3 isoforms. Agave Rca also possessed a much higher in vitro biochemical activity, even at low assay temperatures. Mixtures of rice and agave Rca form functional hetero-oligomers in vitro, but only the rice isoforms denature at nonpermissive temperatures. The high thermostability and activity of agave Rca mapped to the N-terminal 244 residues. A Glu-217-Gln amino acid substitution was found to confer high Rca activity to rice Rca Further mutational analysis suggested that Glu-217 restricts the flexibility of the α4-β4 surface loop that interacts with Rubisco via Lys-216. CAM plants thus promise to be a source of highly functional, thermostable Rca candidates for thermal fortification of crop photosynthesis. Careful characterization of their properties will likely reveal further protein-protein interaction motifs to enrich our mechanistic model of Rca function. © 2017 American Society of Plant Biologists. All Rights Reserved.
Shivhare, Devendra
2017-01-01
To maintain metabolic flux through the Calvin-Benson-Bassham cycle in higher plants, dead-end inhibited complexes of Rubisco must constantly be engaged and remodeled by the molecular chaperone Rubisco activase (Rca). In C3 plants, the thermolability of Rca is responsible for the deactivation of Rubisco and reduction of photosynthesis at moderately elevated temperatures. We reasoned that crassulacean acid metabolism (CAM) plants must possess thermostable Rca to support Calvin-Benson-Bassham cycle flux during the day when stomata are closed. A comparative biochemical characterization of rice (Oryza sativa) and Agave tequilana Rca isoforms demonstrated that the CAM Rca isoforms are approximately10°C more thermostable than the C3 isoforms. Agave Rca also possessed a much higher in vitro biochemical activity, even at low assay temperatures. Mixtures of rice and agave Rca form functional hetero-oligomers in vitro, but only the rice isoforms denature at nonpermissive temperatures. The high thermostability and activity of agave Rca mapped to the N-terminal 244 residues. A Glu-217-Gln amino acid substitution was found to confer high Rca activity to rice Rca. Further mutational analysis suggested that Glu-217 restricts the flexibility of the α4-β4 surface loop that interacts with Rubisco via Lys-216. CAM plants thus promise to be a source of highly functional, thermostable Rca candidates for thermal fortification of crop photosynthesis. Careful characterization of their properties will likely reveal further protein-protein interaction motifs to enrich our mechanistic model of Rca function. PMID:28546437
Xu, Huawei; Zhang, Jianjun; Zeng, Jiwu; Jiang, Linrong; Liu, Ee; Peng, Changlian; He, Zhenghui; Peng, Xinxiang
2009-01-01
Photorespiration is one of the most intensively studied topics in plant biology. While a number of mutants deficient in photorespiratory enzymes have been identified and characterized for their physiological functions, efforts on glycolate oxidase (GLO; EC 1.1.3.15) have not been so successful. This is a report about the generation of transgenic rice (Oryza sativa L.) plants carrying a GLO antisense gene driven by an estradiol-inducible promoter, which allowed for controllable suppressions of GLO and its detailed functional analyses. The GLO-suppressed plants showed typical photorespiration-deficient phenotypes. More intriguingly, it was found that a positive and linear correlation existed between GLO activities and the net photosynthetic rates (P(N)), and photoinhibition subsequently occurred once P(N) reduction surpassed 60%, indicating GLO can exert a strong regulation over photosynthesis. Various expression analyses identified that Rubisco activase was transcriptionally suppressed in the GLO-suppressed plants, consistent with the decreased Rubisco activation states. While the substrate glycolate accumulated substantially, few changes were observed for the product glyoxylate, and for some other downstream metabolites or genes as well in the transgenic plants. Further analyses revealed that isocitrate lyase and malate synthase, two key enzymes in the glyoxylate cycle, were highly up-regulated under GLO deficiency. Taken together, the results suggest that GLO is a typical photorespiratory enzyme and that it can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase, and that the glyoxylate cycle may be partially activated to compensate for the photorespiratory glyoxylate when GLO is suppressed in rice.
Pollock, Steve V; Colombo, Sergio L; Prout, Davey L; Godfrey, Ashley C; Moroney, James V
2003-12-01
This report describes a Chlamydomonas reinhardtii mutant that lacks Rubisco activase (Rca). Using the BleR (bleomycin resistance) gene as a positive selectable marker for nuclear transformation, an insertional mutagenesis screen was performed to select for cells that required a high-CO2 atmosphere for optimal growth. The DNA flanking the BleR insert of one of the high-CO2-requiring strains was cloned using thermal asymmetric interlaced-polymerase chain reaction and inverse polymerase chain reaction and sequenced. The flanking sequence matched the C. reinhardtii Rca cDNA sequence previously deposited in the National Center for Biotechnology Information database. The loss of a functional Rca in the strain was confirmed by the absence of Rca mRNA and protein. The open reading frame for Rca was cloned and expressed in pSL18, a C. reinhardtii expression vector conferring paromomycin resistance. This construct partially complemented the mutant phenotype, supporting the hypothesis that the loss of Rca was the reason the mutant grew poorly in a low-CO2 atmosphere. Sequencing of the C. reinhardtii Rca gene revealed that it contains 10 exons ranging in size from 18 to 470 bp. Low-CO2-grown rca1 cultures had a growth rate and maximum rate of photosynthesis 60% of wild-type cells. Results obtained from experiments on a cia5 rca1 double mutant also suggest that the CO2-concentrating mechanism partially compensates for the absence of an active Rca in the green alga C. reinhardtii.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Glasser, Alan H.
Final technical report on DE-SC0016106. This is the final technical report for a portion of the multi-institutional CEMM project. This report is centered around 3 publications and a seminar presentation, which have been submitted to E-Link.
10 CFR 52.157 - Contents of applications; technical information in final safety analysis report.
Code of Federal Regulations, 2013 CFR
2013-01-01
...; technical information in final safety analysis report. The application must contain a final safety analysis...) Information sufficient to demonstrate compliance with the applicable requirements regarding testing, analysis... 10 Energy 2 2013-01-01 2013-01-01 false Contents of applications; technical information in final...
10 CFR 52.157 - Contents of applications; technical information in final safety analysis report.
Code of Federal Regulations, 2012 CFR
2012-01-01
...; technical information in final safety analysis report. The application must contain a final safety analysis...) Information sufficient to demonstrate compliance with the applicable requirements regarding testing, analysis... 10 Energy 2 2012-01-01 2012-01-01 false Contents of applications; technical information in final...
10 CFR 52.157 - Contents of applications; technical information in final safety analysis report.
Code of Federal Regulations, 2014 CFR
2014-01-01
...; technical information in final safety analysis report. The application must contain a final safety analysis...) Information sufficient to demonstrate compliance with the applicable requirements regarding testing, analysis... 10 Energy 2 2014-01-01 2014-01-01 false Contents of applications; technical information in final...
10 CFR 52.157 - Contents of applications; technical information in final safety analysis report.
Code of Federal Regulations, 2011 CFR
2011-01-01
...; technical information in final safety analysis report. The application must contain a final safety analysis...) Information sufficient to demonstrate compliance with the applicable requirements regarding testing, analysis... 10 Energy 2 2011-01-01 2011-01-01 false Contents of applications; technical information in final...
Ullmann-Zeunert, Lynn; Muck, Alexander; Wielsch, Natalie; Hufsky, Franziska; Stanton, Mariana A; Bartram, Stefan; Böcker, Sebastian; Baldwin, Ian T; Groten, Karin; Svatoš, Aleš
2012-10-05
Herbivory leads to changes in the allocation of nitrogen among different pools and tissues; however, a detailed quantitative analysis of these changes has been lacking. Here, we demonstrate that a mass spectrometric data-independent acquisition approach known as LC-MS(E), combined with a novel algorithm to quantify heavy atom enrichment in peptides, is able to quantify elicited changes in protein amounts and (15)N flux in a high throughput manner. The reliable identification/quantitation of rabbit phosphorylase b protein spiked into leaf protein extract was achieved. The linear dynamic range, reproducibility of technical and biological replicates, and differences between measured and expected (15)N-incorporation into the small (SSU) and large (LSU) subunits of ribulose-1,5-bisphosphate-carboxylase/oxygenase (RuBisCO) and RuBisCO activase 2 (RCA2) of Nicotiana attenuata plants grown in hydroponic culture at different known concentrations of (15)N-labeled nitrate were used to further evaluate the procedure. The utility of the method for whole-plant studies in ecologically realistic contexts was demonstrated by using (15)N-pulse protocols on plants growing in soil under unknown (15)N-incorporation levels. Additionally, we quantified the amounts of lipoxygenase 2 (LOX2) protein, an enzyme important in antiherbivore defense responses, demonstrating that the approach allows for in-depth quantitative proteomics and (15)N flux analyses of the metabolic dynamics elicited during plant-herbivore interactions.
Lysine acetylome profiling uncovers novel histone deacetylase substrate proteins in Arabidopsis.
Hartl, Markus; Füßl, Magdalena; Boersema, Paul J; Jost, Jan-Oliver; Kramer, Katharina; Bakirbas, Ahmet; Sindlinger, Julia; Plöchinger, Magdalena; Leister, Dario; Uhrig, Glen; Moorhead, Greg Bg; Cox, Jürgen; Salvucci, Michael E; Schwarzer, Dirk; Mann, Matthias; Finkemeier, Iris
2017-10-23
Histone deacetylases have central functions in regulating stress defenses and development in plants. However, the knowledge about the deacetylase functions is largely limited to histones, although these enzymes were found in diverse subcellular compartments. In this study, we determined the proteome-wide signatures of the RPD3/HDA1 class of histone deacetylases in Arabidopsis Relative quantification of the changes in the lysine acetylation levels was determined on a proteome-wide scale after treatment of Arabidopsis leaves with deacetylase inhibitors apicidin and trichostatin A. We identified 91 new acetylated candidate proteins other than histones, which are potential substrates of the RPD3/HDA1-like histone deacetylases in Arabidopsis , of which at least 30 of these proteins function in nucleic acid binding. Furthermore, our analysis revealed that histone deacetylase 14 (HDA14) is the first organellar-localized RPD3/HDA1 class protein found to reside in the chloroplasts and that the majority of its protein targets have functions in photosynthesis. Finally, the analysis of HDA14 loss-of-function mutants revealed that the activation state of RuBisCO is controlled by lysine acetylation of RuBisCO activase under low-light conditions. © 2017 The Authors. Published under the terms of the CC BY 4.0 license.
Kuriata, Agnieszka M; Chakraborty, Manas; Henderson, J Nathan; Hazra, Suratna; Serban, Andrew J; Pham, Tuong V T; Levitus, Marcia; Wachter, Rebekka M
2014-11-25
We report a fluorescence correlation spectroscopy (FCS) study of the assembly pathway of the AAA+ protein ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase (Rca), a ring-forming ATPase responsible for activation of inhibited Rubisco complexes for biological carbon fixation. A thermodynamic characterization of simultaneously populated oligomeric states appears critical in understanding Rca structure and function. Using cotton β-Rca, we demonstrate that apparent diffusion coefficients vary as a function of concentration, nucleotide, and cation. Using manual fitting procedures, we provide estimates for the equilibrium constants for the stepwise assembly and find that in the presence of ATPγS, the Kd for hexamerization is 10-fold lower than with ADP (∼0.1 vs ∼1 μM). Hexamer fractions peak at 30 μM and dominate at 8-70 μM Rca, where they comprise 60-80% of subunits with ATPγS, compared with just 30-40% with ADP. Dimer fractions peak at 1-4 μM Rca, where they comprise 15-18% with ATPγS and 26-28% with ADP. At 30 μM Rca, large aggregates begin to form that comprise ∼10% of total protein with ATPγS and ∼25% with ADP. FCS data collected on the catalytically impaired WalkerB-D173N variant in the presence of ATP provided strong support for these results. Titration with free magnesium ions lead to the disaggregation of larger complexes in favor of hexameric forms, suggesting that a second magnesium binding site with a Kd value of 1-3 mM mediates critical subunit contacts. We propose that closed-ring toroidal hexameric forms are stabilized by binding of Mg·ATP plus Mg2+, whereas Mg·ADP promotes continuous assembly to supramolecular aggregates such as spirals.
Esquivel, M Gloria; Genkov, Todor; Nogueira, Ana S; Salvucci, Michael E; Spreitzer, Robert J
2013-12-01
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyzes the initial step of carbon metabolism in photosynthesis. The holoenzyme comprises eight large subunits, arranged as a tetramer of dimers around a central solvent channel that defines a fourfold axis of symmetry, and eight small subunits, arranged as two tetramers at the poles of the axis. The phylogenetically divergent small-subunit loops between β-strands A and B form the entrance to the solvent channel. In the green alga Chlamydomonas reinhardtii, Ile-58 from each of the four small-subunit βA-βB loops defines the minimal diameter of the channel opening. To understand the role of the central solvent channel in Rubisco function, directed mutagenesis and transformation of Chlamydomonas were employed to replace Ile-58 with Ala, Lys, Glu, Trp, or three Trp residues (I58W3) to close the entrance to the channel. The I58E, I58K, and I58W substitutions caused only small decreases in photosynthetic growth at 25 and 35 °C, whereas I58W3 had a substantial effect at both temperatures. The mutant enzymes had decreased carboxylation rates, but the I58W3 enzyme had decreases in both carboxylation and CO2/O2 specificity. The I58E, I58W, and I58W3 enzymes were inactivated at lower temperatures than wild-type Rubisco, and were degraded at slower rates under oxidative stress. However, these mutant enzymes were activated by Rubisco activase at normal rates, indicating that the structural transition required for carboxylation is not affected by altering the solvent channel opening. Structural dynamics alone may not be responsible for these distant effects on the Rubisco active site.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Yang, Zhipan; Lu, Qingtao; Wen, Xiaogang
Highlights: Black-Right-Pointing-Pointer Rice rubisco activase promoter was analyzed in transgenic Arabidopsis system. Black-Right-Pointing-Pointer Region conferring tissue specific and light inducible expression of Rca was identified. Black-Right-Pointing-Pointer -58 to +43 bp region mediates tissue-specific expression of rice Rca. Black-Right-Pointing-Pointer Light inducible expression of rice Rca is mediated by -297 to -58 bp region. Black-Right-Pointing-Pointer Rice nuclear proteins bind specifically with the light inducible region. -- Abstract: To gain a better understanding of the regulatory mechanism of the rice rubisco activase (Rca) gene, variants of the Rca gene promoter (one full-length and four deletion mutants) fused to the coding region of themore » bacterial reporter gene {beta}-glucuronidase (GUS) were introduced into Arabidopsis via Agrobacterium-mediated transformation. Our results show that a 340 bp fragment spanning from -297 to +43 bp relative to the transcription initiation site is enough to promote tissue-specific and light-inducible expression of the rice Rca gene as done by the full-length promoter (-1428 to +43 bp). Further deletion analysis indicated that the region conferring tissue-specificity of Rca expression is localized within a 105 bp fragment from -58 to +43 bp, while light-inducible expression of Rca is mediated by the region from -297 to -58 bp. Gel shift assays and competition experiments demonstrated that rice nuclear proteins bind specifically with the fragment conferring light responsiveness at more than one binding site. This implies that multiple cis-elements may be involved in light-induced expression of the rice Rca gene. These works provide a useful reference for understanding transcriptional regulation mechanism of the rice Rca gene, and lay a strong foundation for further detection of related cis-elements and trans-factors.« less
Regulation of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) Activase
Hazra, Suratna; Henderson, J. Nathan; Liles, Kevin; Hilton, Matthew T.; Wachter, Rebekka M.
2015-01-01
In many photosynthetic organisms, tight-binding Rubisco inhibitors are released by the motor protein Rubisco activase (Rca). In higher plants, Rca plays a pivotal role in regulating CO2 fixation. Here, the ATPase activity of 0.005 mm tobacco Rca was monitored under steady-state conditions, and global curve fitting was utilized to extract kinetic constants. The kcat was best fit by 22.3 ± 4.9 min−1, the Km for ATP by 0.104 ± 0.024 mm, and the Ki for ADP by 0.037 ± 0.007 mm. Without ADP, the Hill coefficient for ATP hydrolysis was extracted to be 1.0 ± 0.1, indicating noncooperative behavior of homo-oligomeric Rca assemblies. However, the addition of ADP was shown to introduce positive cooperativity between two or more subunits (Hill coefficient 1.9 ± 0.2), allowing for regulation via the prevailing ATP/ADP ratio. ADP-mediated activation was not observed, although larger amounts led to competitive product inhibition of hydrolytic activity. The catalytic efficiency increased 8.4-fold upon cooperative binding of a second magnesium ion (Hill coefficient 2.5 ± 0.5), suggesting at least three conformational states (ATP-bound, ADP-bound, and empty) within assemblies containing an average of about six subunits. The addition of excess Rubisco (24:1, L8S8/Rca6) and crowding agents did not modify catalytic rates. However, high magnesium provided for thermal Rca stabilization. We propose that magnesium mediates the formation of closed hexameric toroids capable of high turnover rates and amenable to allosteric regulation. We suggest that in vivo, the Rca hydrolytic activity is tuned by fluctuating [Mg2+] in response to changes in available light. PMID:26283786
The Diverse AAA+ Machines that Repair Inhibited Rubisco Active Sites
Mueller-Cajar, Oliver
2017-01-01
Gaseous carbon dioxide enters the biosphere almost exclusively via the active site of the enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco). This highly conserved catalyst has an almost universal propensity to non-productively interact with its substrate ribulose 1,5-bisphosphate, leading to the formation of dead-end inhibited complexes. In diverse autotrophic organisms this tendency has been counteracted by the recruitment of dedicated AAA+ (ATPases associated with various cellular activities) proteins that all use the energy of ATP hydrolysis to remodel inhibited Rubisco active sites leading to release of the inhibitor. Three evolutionarily distinct classes of these Rubisco activases (Rcas) have been discovered so far. Green and red-type Rca are mostly found in photosynthetic eukaryotes of the green and red plastid lineage respectively, whereas CbbQO is associated with chemoautotrophic bacteria. Ongoing mechanistic studies are elucidating how the various motors are utilizing both similar and contrasting strategies to ultimately perform their common function of cracking the inhibited Rubisco active site. The best studied mechanism utilized by red-type Rca appears to involve transient threading of the Rubisco large subunit C-terminal peptide, reminiscent of the action performed by Clp proteases. As well as providing a fascinating example of convergent molecular evolution, Rca proteins can be considered promising crop-improvement targets. Approaches aiming to replace Rubisco in plants with improved enzymes will need to ensure the presence of a compatible Rca protein. The thermolability of the Rca protein found in crop plants provides an opportunity to fortify photosynthesis against high temperature stress. Photosynthesis also appears to be limited by Rca when light conditions are fluctuating. Synthetic biology strategies aiming to enhance the autotrophic CO2 fixation machinery will need to take into consideration the requirement for Rubisco activases as well as their properties. PMID:28580359
Hazra, Suratna; Henderson, J Nathan; Liles, Kevin; Hilton, Matthew T; Wachter, Rebekka M
2015-10-02
In many photosynthetic organisms, tight-binding Rubisco inhibitors are released by the motor protein Rubisco activase (Rca). In higher plants, Rca plays a pivotal role in regulating CO2 fixation. Here, the ATPase activity of 0.005 mm tobacco Rca was monitored under steady-state conditions, and global curve fitting was utilized to extract kinetic constants. The kcat was best fit by 22.3 ± 4.9 min(-1), the Km for ATP by 0.104 ± 0.024 mm, and the Ki for ADP by 0.037 ± 0.007 mm. Without ADP, the Hill coefficient for ATP hydrolysis was extracted to be 1.0 ± 0.1, indicating noncooperative behavior of homo-oligomeric Rca assemblies. However, the addition of ADP was shown to introduce positive cooperativity between two or more subunits (Hill coefficient 1.9 ± 0.2), allowing for regulation via the prevailing ATP/ADP ratio. ADP-mediated activation was not observed, although larger amounts led to competitive product inhibition of hydrolytic activity. The catalytic efficiency increased 8.4-fold upon cooperative binding of a second magnesium ion (Hill coefficient 2.5 ± 0.5), suggesting at least three conformational states (ATP-bound, ADP-bound, and empty) within assemblies containing an average of about six subunits. The addition of excess Rubisco (24:1, L8S8/Rca6) and crowding agents did not modify catalytic rates. However, high magnesium provided for thermal Rca stabilization. We propose that magnesium mediates the formation of closed hexameric toroids capable of high turnover rates and amenable to allosteric regulation. We suggest that in vivo, the Rca hydrolytic activity is tuned by fluctuating [Mg(2+)] in response to changes in available light. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
Woodrow, Ian E.; Mott, Keith A.
1992-01-01
The activation kinetics of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) following an increase in photon flux density (PFD) were studied by analyzing CO2 assimilation time courses in spinach leaves (Spinacia oleracea). When leaves were exposed to 45 minutes of darkness before illumination at 690 micromoles per square meter per second, Rubisco activation followed apparent first-order kinetics with a relaxation time of about 3.8 minutes. But when leaves were illuminated for 45 minutes at 160 micromoles per square meter per second prior to illumination at 690 micromoles per square meter per second the relaxation time for Rubisco activation was only 2.1 minutes. The kinetics of this change in relaxation times were investigated by exposing dark-adapted leaves to 160 micromoles per square meter per second for different periods before increasing the PFD to 690 micromoles per square meter per second. It was found that the apparent relaxation time for Rubisco activation changed from 3.8 to 2.1 minutes slowly, requiring at least 8 minutes for completion. This result indicates that at least two sequential, slow processes are involved in light-mediated activation of Rubisco in spinach leaves and that the relaxation times characterizing these two processes are about 4 and 2 minutes, respectively. The kinetics of the first process in the reverse direction and the dependence of the relaxation time for the second process on the magnitude of the increase in PFD were also determined. Evidence that the first slow process is activation of the enzyme Rubisco activase and that the second slow process is the catalytic activation of Rubisco by activase is discussed. PMID:16668865
Carmo-Silva, A Elizabete; Salvucci, Michael E
2013-04-01
Rubisco's catalytic chaperone, Rubisco activase (Rca), uses the energy from ATP hydrolysis to restore catalytic competence to Rubisco. In Arabidopsis (Arabidopsis thaliana), inhibition of Rca activity by ADP is fine tuned by redox regulation of the α-isoform. To elucidate the mechanism for Rca regulation in species containing only the redox-insensitive β-isoform, the response of activity to ADP was characterized for different Rca forms. When assayed in leaf extracts, Rubisco activation was significantly inhibited by physiological ratios of ADP to ATP in species containing both α-Rca and β-Rca (Arabidopsis and camelina [Camelina sativa]) or just the β-Rca (tobacco [Nicotiana tabacum]). However, Rca activity was insensitive to ADP inhibition in an Arabidopsis transformant, rwt43, which expresses only Arabidopsis β-Rca, although not in a transformant of Arabidopsis that expresses a tobacco-like β-Rca. ATP hydrolysis by recombinant Arabidopsis β-Rca was much less sensitive to inhibition by ADP than recombinant tobacco β-Rca. Mutation of 17 amino acids in the tobacco β-Rca to the corresponding Arabidopsis residues reduced ADP sensitivity. In planta, Rubisco deactivated at low irradiance except in the Arabidopsis rwt43 transformant containing an ADP-insensitive Rca. Induction of CO2 assimilation after transition from low to high irradiance was much more rapid in the rwt43 transformant compared with plants containing ADP-sensitive Rca forms. The faster rate of photosynthetic induction and a greater enhancement of growth under a fluctuating light regime by the rwt43 transformant compared with wild-type Arabidopsis suggests that manipulation of Rca regulation might provide a strategy for enhancing photosynthetic performance in certain variable light environments.
Carmo-Silva, A Elizabete; Salvucci, Michael E
2012-11-01
The temperature optimum of photosynthesis coincides with the average daytime temperature in a species' native environment. Moderate heat stress occurs when temperatures exceed the optimum, inhibiting photosynthesis and decreasing productivity. In the present study, the temperature response of photosynthesis and the potential for heat acclimation was evaluated for Camelina sativa, a bioenergy crop. The temperature optimum of net CO(2) assimilation rate (A) under atmospheric conditions was 30-32 °C and was only slightly higher under non-photorespiratory conditions. The activation state of Rubisco was closely correlated with A at supra-optimal temperatures, exhibiting a parallel decrease with increasing leaf temperature. At both control and elevated temperatures, the modeled response of A to intercellular CO(2) concentration was consistent with Rubisco limiting A at ambient CO(2). Rubisco activation and photochemical activities were affected by moderate heat stress at lower temperatures in camelina than in the warm-adapted species cotton and tobacco. Growth under conditions that imposed a daily interval of moderate heat stress caused a 63 % reduction in camelina seed yield. Levels of cpn60 protein were elevated under the higher growth temperature, but acclimation of photosynthesis was minimal. Inactivation of Rubisco in camelina at temperatures above 35 °C was consistent with the temperature response of Rubisco activase activity and indicated that Rubisco activase was a prime target of inhibition by moderate heat stress in camelina. That photosynthesis exhibited no acclimation to moderate heat stress will likely impact the development of camelina and other cool season Brassicaceae as sources of bioenergy in a warmer world.
Henry Taube and Coordination Chemistry
Shifts Caused by Cr++ in Aqueous Solutions, DOE Technical Report, 1962 Reactions of Solvated Ions Final Report, DOE Technical Report, 1962 Isotopic Discrimination of Some Solutes in Liquid Ammonia, DOE Technical Report, 1966 Final Technical Report of Research, DOE Technical Report, 1972 Top Additional Web
George A. Olah, Carbocation and Hydrocarbon Chemistry
. Final Technical Report. [HF:BF{sub 2}/H{sub 2}] , DOE Technical Report, 1980 Superacid Catalyzed Coal Conversion Chemistry. 1st and 2nd Quarterly Technical Progress Reports, September 1, 1983-March 30, 1984 , DOE Technical Report, 1984 Superacid Catalyzed Coal Conversion Chemistry. Final Technical Report
77 FR 46306 - Fluxapyroxad; Pesticide Tolerances Technical Amendment
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...; Pesticide Tolerances Technical Amendment AGENCY: Environmental Protection Agency (EPA). ACTION: Final rule; technical amendment. SUMMARY: EPA issued a final rule in the Federal Register of May 14, 2012, concerning.... Inadvertently, the terminology for the oilseed crop group and for dried plums was incorrect. This technical...
Code of Federal Regulations, 2014 CFR
2014-07-01
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76 FR 18624 - Research, Technical Assistance and Training Programs: Notice of Final Circular
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... to FTA Circular 6100.1D, Research and Technical Assistance Training Program: Application Instructions... DEPARTMENT OF TRANSPORTATION Federal Transit Administration Research, Technical Assistance and Training Programs: Notice of Final Circular AGENCY: Federal Transit Administration (FTA), DOT. ACTION...
Minnesota Deaf-Blind Technical Assistance Project. Final Report.
ERIC Educational Resources Information Center
Kloos, Eric
This final report describes activities and accomplishments of the 3-year federally supported Minnesota Deaf-Blind Technical Assistance Project. The project provided training and technical assistance, information sharing, and support services to families of children with deaf-blindness. Activities and accomplishments included: collaboration with…
75 FR 56857 - Pilot, Flight Instructor, and Pilot School Certification
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2010-09-17
... of part 141. Discussion of Technical Amendment Section 141.5(d) establishes the quality of training... Certification AGENCY: Federal Aviation Administration, DOT. ACTION: Final rule; technical amendment. SUMMARY: The Federal Aviation Administration (FAA) is making minor technical changes to a final rule published...
77 FR 29247 - Federal Motor Vehicle Safety Standards; Occupant Crash Protection
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2012-05-17
...). ACTION: Final rule; technical amendments. SUMMARY: This final rule makes technical amendments to Federal... advanced air bag requirements. As written now, the general warning label requirements contain an explicit... equipment requirements for restraint systems. This document makes technical amendments to several of the...
Kumar, Ranjeet R; Goswami, Suneha; Singh, Khushboo; Dubey, Kavita; Singh, Shweta; Sharma, Renu; Verma, Neeraj; Kala, Yugal K; Rai, Gyanendra K; Grover, Monendra; Mishra, Dwijesh C; Singh, Bhupinder; Pathak, Himanshu; Chinnusamy, Viswanathan; Rai, Anil; Praveen, Shelly
2016-01-01
RuBisCo activase (Rca) is a catalytic chaperone involved in modulating the activity of RuBisCo (key enzyme of photosynthetic pathway). Here, we identified eight novel transcripts from wheat through data mining predicted to be Rca and cloned a transcript of 1.4 kb from cv. HD2985, named as TaRca1 (GenBank acc. no. KC776912). Single copy number of TaRca1 was observed in wheat genome. Expression analysis in diverse wheat genotypes (HD2985, Halna, PBW621, and HD2329) showed very high relative expression of TaRca1 in Halna under control and HS-treated, as compared to other cultivars at different stages of growth. TaRca1 protein was predicted to be chloroplast-localized with numerous potential phosphorylation sites. Northern blot analysis showed maximum accumulation of TaRca1 transcript in thermotolerant cv. during mealy-ripe stage, as compared to thermosusceptible. Decrease in the photosynthetic parameters was observed in all the cultivars, except PBW621 in response to HS. We observed significant increase in the Rca activity in all the cultivars under HS at different stages of growth. HS causes decrease in the RuBisCo activity; maximum reduction was observed during pollination stage in thermosusceptible cvs. as validated through immunoblotting. We observed uniform carbon distribution in different tissues of thermotolerant cvs., as compared to thermosusceptible. Similarly, tolerance level of leaf was observed maximum in Halna having high Rca activity under HS. A positive correlation was observed between the transcript and activity of TaRca1 in HS-treated Halna. Similarly, TaRca1 enzyme showed positive correlation with the activity of RuBisCo. There is, however, need to manipulate the thermal stability of TaRca1 enzyme through protein engineering for sustaining the photosynthetic rate under HS-a novel approach toward development of "climate-smart" crop.
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48 CFR 1852.235-73 - Final Scientific and Technical Reports.
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1991-02-01
to adequately assess the health and environmental risks associated with the closure and transfer of the Annex forI other use; and 3) identification of...1990); Draft Final Technical Plan, Draft Final Sampling Design Plan and Draft Final Health and Safety Plan, USATHAMA, June 1990. 2.1.2 Draft Final...Final Technical Plan, Sampling Design Plan and Health and Safety Plan) supplied by USATHAMA. The estimate may be revised, with USATHAMA approval, as
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...: Final rule. SUMMARY: This final rule makes technical changes that will update a requirement that many of the written agreements and memoranda of understanding (MOUs) between the Food and Drug Administration.... This final rule, accordingly, eliminates it. We are making these technical changes to conserve Agency...
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2005 v4.3 Technical Support Document
Emissions Modeling for the Final Mercury and Air Toxics Standards Technical Support Document describes how updated 2005 NEI, version 2 emissions were processed for air quality modeling in support of the final Mercury and Air Toxics Standards (MATS).
The Center for Space Telemetering and Telecommunications Systems
NASA Technical Reports Server (NTRS)
Horan, S.; DeLeon, P.; Borah, D.; Lyman, R.
2003-01-01
This report comprises the final technical report for the research grant 'Center for Space Telemetering and Telecommunications Systems' sponsored by the National Aeronautics and Space Administration's Goddard Space Flight Center. The grant activities are broken down into the following technology areas: (1) Space Protocol Testing; (2) Autonomous Reconfiguration of Ground Station Receivers; (3) Satellite Cluster Communications; and (4) Bandwidth Efficient Modulation. The grant activity produced a number of technical reports and papers that were communicated to NASA as they were generated. This final report contains the final summary papers or final technical report conclusions for each of the project areas. Additionally, the grant supported students who made progress towards their degrees while working on the research.
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United Nations Educational, Scientific, and Cultural Organization, Paris (France).
This monograph includes the final report of the International Expert Meeting on the Promotion of Equal Access of Girls and Women to Technical and Vocational Education (TVE) held in Seoul, Republic of Korea, and country discussion papers. The final report is composed of an introduction that proposes that many Member States require special measures…
Air Quality Modeling Technical Support Document for the Final Cross State Air Pollution Rule Update
In this technical support document (TSD) we describe the air quality modeling performed to support the final Cross State Air Pollution Rule for the 2008 ozone National Ambient Air Quality Standards (NAAQS).
7 CFR 614.7 - Preliminary technical determinations.
Code of Federal Regulations, 2010 CFR
2010-01-01
... 7 Agriculture 6 2010-01-01 2010-01-01 false Preliminary technical determinations. 614.7 Section... Preliminary technical determinations. (a) A preliminary technical determination becomes final 30 days after... purpose of gathering additional information and discussing the facts relating to the preliminary technical...
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Hammond, Cathy; Drew, Sam F.; Withington, Cairen; Griffith, Cathy; Swiger, Caroline M.; Mobley, Catherine; Sharp, Julia L.; Stringfield, Samuel C.; Stipanovic, Natalie; Daugherty, Lindsay
2013-01-01
This is the final technical report from the National Research Center for Career and Technical Education's (NRCCTE's) five-year longitudinal study of South Carolina's Personal Pathway to Success initiative, which was authorized by the state's Education and Economic Development Act (EEDA) in 2005. NRCCTE-affiliated researchers at the National…
Direct Final Rule: Nonroad Diesel Technical Amendments and Tier 3 Technical Relief Provision
Rule making certain technical corrections to the rules establishing emission standards for nonroad diesel engines and amending those rules to provide manufacturers with a production technical relief provision for Tier 3 equipment.
Arizona Deafblind Project, 1995-1999. Final Report.
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Arizona State School for the Deaf and Blind, Tucson.
This final report describes accomplishments of the four-year federally funded Arizona Deafblind Project which attempted to: (1) identify all deafblind children in Arizona; (2) deliver technical assistance to families; (3) deliver technical assistance to service providers; and (4) enhance community oversight, coordination, and collaboration with…
Joint Common Architecture Demonstration (JCA Demo) Final Report
2016-07-28
approach for implementing open systems [16], formerly known as the Modular Open Systems Approach (MOSA). OSA is a business and technical strategy to... TECHNICAL REPORT RDMR-AD-16-01 JOINT COMMON ARCHITECTURE DEMONSTRATION (JCA DEMO) FINAL REPORT Scott A. Wigginton... Modular Avionics .......................................................................... 5 E. Model-Based Engineering
76 FR 80226 - Technical Amendments
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"Type Ia Supernovae: Tools for Studying Dark Energy" Final Technical Report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Woosley, Stan; Kasen, Dan
2017-05-10
Final technical report for project "Type Ia Supernovae: Tools for the Study of Dark Energy" awarded jointly to scientists at the University of California, Santa Cruz and Berkeley, for computer modeling, theory and data analysis relevant to the use of Type Ia supernovae as standard candles for cosmology.
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TADS Final Evaluation Report, 1980-81. Appendix S.
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Suarez, Tanya M.; And Others
The document contains the final report of the Technical Assistance Development System (TADS), a program which provided technical assistance (TA) services to 53 Handicapped Children's Early Education Program (HCEEP) demonstration projects and 13 State Implementation Grants (SIGs). The evaluation report is divided into five sections. Section 1…
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...: 84.326Z.] Final Waiver and Extension of the Project Period for the Technical Assistance Coordination... project period. SUMMARY: The Secretary waives the requirements in the Education Department General Administrative Regulations that generally prohibit project periods exceeding five years and extensions of project...
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...: Direct final rule. SUMMARY: This direct final rule makes technical changes that will update a requirement that many of our written agreements and memoranda of understanding (MOUs) with other departments..., accordingly, eliminates it. We are making these technical changes to conserve Agency time and resources...
EPA's 1988 regulations concerning USTs are contained in 40 CFR Part 280, 40 CFR Part 281 and 40 CFR Parts 282.50-282.105 and divided into three sections: technical requirements, financial responsibility requirements, and state program approval objectives.
PITTSBURGH TECHNICAL HEALTH TRAINING INSTITUTE DEMONSTRATION PROJECT. FINAL REPORT, VOLUME II.
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KISHKUNAS, LOUIS J.
APPENDIXES TO THE "FINAL REPORT," VOLUME I (VT 005 511), ARE INCLUDED--(1) A SCHEMATIC REPRESENTATION OF CURRICULUM DEVELOPMENT, (2) TECHNICAL BEHAVIOR CHECKLISTS, (3) PERFORMANCE INVENTORY FORMS USED IN ON-THE-JOB OBSERVATIONS, (4) REPORT FORM FOR TYPICAL JOB BEHAVIOR OF EMPLOYEE, (5) COOPERATING AREA HEALTH INSTITUTIONS, (6) TABLES OF Z SCORES…
Invention and Writing in Technical Work: Representing the Object.
ERIC Educational Resources Information Center
Winsor, Dorothy A.
1994-01-01
Describes the way invention is relevant to the practice of technical writing. Studies three engineering students engaged in a real-world project. Shows how the students' technical work and invention for the final report were simultaneous activities. Claims that invention for and through writing overlaps with technical invention. (HB)
Project T.E.A.M. (Technical Education Advancement Modules). Final Report.
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Greenville Technical Coll., SC.
Project TEAM (Technical Education Advancement Modules), a cooperative demonstration program for high technology training, created an introductory technical training program and a consumer education package emphasizing the benefits of technical training. The curriculum and training focus of the project began with an assessment of employee needs in…
77 FR 39623 - Airworthiness Standards: Aircraft Engines; Technical Amendment
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2012-07-05
...] Airworthiness Standards: Aircraft Engines; Technical Amendment AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule; technical amendment. SUMMARY: This amendment clarifies aircraft engine... from applicants requesting FAA engine type certifications and aftermarket certifications, such as...
7 CFR 652.36 - Appeal of decertification decisions.
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2010-01-01
... technical service provider's written appeal, the Chief or his designee, will make a final determination, in... CONSERVATION SERVICE, DEPARTMENT OF AGRICULTURE SUPPORT ACTIVITIES TECHNICAL SERVICE PROVIDER ASSISTANCE... of the State Conservationist's decertification determination, the technical service provider may...
Capturing Cognitive Fingerprints for Active Authentication
2014-10-01
CAPTURING COGNITIVE FINGERPRINTS FOR ACTIVE AUTHENTICATION IOWA STATE UNIVERSITY OF SCIENCE & TECHNOLOGY OCTOBER 2014 FINAL TECHNICAL REPORT...REPORT TYPE FINAL TECHNICAL REPORT 3. DATES COVERED (From - To) SEP 2013 – APR 2014 4. TITLE AND SUBTITLE CAPTURING COGNITIVE FINGERPRINTS FOR ACTIVE...The project ended before the IRB application was approved. 15. SUBJECT TERMS Active Authentication, Cognitive Fingerprints , Biometric Modalities
ERIC Educational Resources Information Center
Morgan, Robert P.
Research is summarized in a brief final report built around a four-section bibliography. The first section lists periodic progress reports and articles which provide an overview of the program, including articles which pertain primarily to educational rather than technical aspects of satellite utilization. Theses carried out in the fields of…
Head Start Impact Study. Technical Report
ERIC Educational Resources Information Center
Puma, Michael; Bell, Stephen; Cook, Ronna; Heid, Camilla; Shapiro, Gary; Broene, Pam; Jenkins, Frank; Fletcher, Philip; Quinn, Liz; Friedman, Janet; Ciarico, Janet; Rohacek, Monica; Adams, Gina; Spier, Elizabeth
2010-01-01
This Technical Report is designed to provide technical detail to support the analysis and findings presented in the "Head Start Impact Study Final Report" (U.S. Department of Health and Human Services, January 2010). Chapter 1 provides an overview of the Head Start Impact Study and its findings. Chapter 2 provides technical information on the…
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Sommers, Paul; Heg, Deena
A project was conducted to improve the state of Washington's community and technical college system by developing and using an improved occupational forecasting system to assess and respond to education and training needs. First, long-term occupational forecast data from Washington's Employment Security Department were matched with technical and…
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... Preparation of Market-Based Rate Filings and Electric Quarterly Reports by Public Utilities; Notice of Technical Conference January 28, 2010. Take notice that Commission staff will convene a technical conference... final agenda of the technical conference. The March 3, 2010 technical conference will focus on the...
A Revision of Technical Mathematics Based on the NCTM Standards. Final Report.
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Near, Barbara
Between 1993 and 1996, Henry Ford Community College (Michigan) worked with business, industry, and technical instructors to revise their Technical Mathematics program in accordance with the National Council of Teachers of Mathematics (NCTM) Standards. The purpose of the project was to restructure the technical math curriculum and create a context…
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... SECURITIES AND EXCHANGE COMMISSION 17 CFR Part 240 [Release No. 34-63949] Technical Amendments to...: Securities and Exchange Commission. ACTION: Final rule; technical amendments. SUMMARY: The Securities and Exchange Commission (``Commission'') is adopting technical amendments to Rule 17a-8 under the Securities...
2013-05-20
The Assistant Secretary for Special Education and Rehabilitative Services announces a priority under the Technical Assistance to Improve State Data Capacity program. The Assistant Secretary may use this priority for competitions in fiscal year (FY) 2013 and later years. We take this action to focus attention on an identified national need to provide technical assistance (TA) to States to improve their capacity to meet the data collection and reporting requirements of the Individuals with Disabilities Education Act (IDEA). We intend this priority to establish a TA center to improve State capacity to accurately collect and report IDEA data (Data Center).
Technical Evaluation Motor No. 10 (TEM-10)
NASA Technical Reports Server (NTRS)
1993-01-01
Technical Evaluation Motor No. 10 (TEM-10) was static fired on 27 Apr. 1993 at the Thiokol Corporation full-scale motor static test bay, T-24. This final test report documents the procedures, performance, and results of the static test firing of TEM-10. All observations, discussions, conclusions, and recommendations contained are final. Included is a presentation and discussion of TEM-10 performance, anomalies, and test results in concurrence with the objectives outlined in CTP-0110, Revision D, Space Shuttle Technical Evaluation Motor No. 10 (TEM-10) Static Fire Test Plan.
2014-08-05
The Assistant Secretary for the Office of Special Education and Rehabilitative Services (OSERS) announces a priority under the Technical Assistance on State Data Collection program. The Assistant Secretary may use this priority for competitions in fiscal year (FY) 2014 and later years. We take this action to fund a cooperative agreement to establish and operate an IDEA Data Management Center (Center) that will provide technical assistance (TA) to improve the capacity of States to meet the data collection requirements of the Individuals with Disabilities Education Act (IDEA).
2016-05-31
and included explosives such as TATP, HMTD, RDX, RDX, ammonium nitrate , potassium perchlorate, potassium nitrate , sugar, and TNT. The approach...Distribution Unlimited UU UU UU UU 31-05-2016 15-Apr-2014 14-Jan-2015 Final Report: Technical Topic 3.2.2. d Bayesian and Non- parametric Statistics...of Papers published in non peer-reviewed journals: Final Report: Technical Topic 3.2.2. d Bayesian and Non-parametric Statistics: Integration of Neural
Final June Revisions Rule Significant Contribution Assessment TSD
This Technical Support Document (TSD) presents quantitative assessments of the relationship between the final February revisions to the Transport Rule, the final June revisions rule, and the original analysis conducted for the final Transport Rule.
2006-05-30
implementation Final Report 4 TECHNICAL PLAN AND RESULTS Task 1: Initiate the Project Management System Two senior NGSS production management...1 Technical Plan and Results...Third the system is hosted on a handheld unit which provides the foremen with an efficient daily planning tool. The Pilot System which entails
ERIC Educational Resources Information Center
North Central Technical Inst., Wausau, WI.
This final report contains the program proposal with supporting data for developing curriculum materials for and implementing an associate-degree laser technology program at the North Central Technical Institute. The proposal outline provides this information: (1) objectives for the program designed to prepare a technician to safely operate,…
ERIC Educational Resources Information Center
Goertz, Lori; Franklin, Barbara
This final report describes the activities and outcomes of the California Deaf-Blind Services (CDBS) program, a regionally based, family focused technical assistance and training project designed to improve services to children with deaf-blindness. The project conducted the following activities: (1) provided technical assistance to families and…
ERIC Educational Resources Information Center
New Jersey State Dept. of Education, Trenton.
This final report describes activities and accomplishments of the New Jersey Technical Assistance Project, a project to improve educational resources and support services for students with multiple sensory impairment (deaf-blindness). Activities and accomplishments are presented in a tabular format for each project goal and objective. The project…
78 FR 77563 - Technical Amendments
Federal Register 2010, 2011, 2012, 2013, 2014
2013-12-24
... NATIONAL CREDIT UNION ADMINISTRATION 12 CFR Parts 700, 701, and 704 RIN 3133-AE33 Technical Amendments AGENCY: National Credit Union Administration (NCUA). ACTION: Final rule. SUMMARY: The NCUA Board... credit unions. The technical amendments conform the regulations to a recent policy change adopted by the...
75 FR 33682 - Export Administration Regulations; Technical Amendments
Federal Register 2010, 2011, 2012, 2013, 2014
2010-06-15
...-01] RIN 0694-AE93 Export Administration Regulations; Technical Amendments AGENCY: Bureau of Industry... Bureau of Industry and Security (BIS) makes a technical amendment to the Export Administration... review of final decisions and orders issued in BIS export control administrative enforcement proceedings...
78 FR 41331 - Defense Federal Acquisition Regulation Supplement; Technical Amendments
Federal Register 2010, 2011, 2012, 2013, 2014
2013-07-10
... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 225 Defense Federal Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Final rule. SUMMARY: DoD is making technical amendment to the Defense...
Final Revisions Rule Significant Contribution Assessment TSD
This Technical Support Document (TSD) presents quantitative assessments of the relationship between final revisions to the Transport Rule and the original analysis conducted for the final Transport Rule.
75 FR 15342 - Advisory Committees; Technical Amendment
Federal Register 2010, 2011, 2012, 2013, 2014
2010-03-29
... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration 21 CFR Part 14 [Docket No. FDA-2010-N-0001] Advisory Committees; Technical Amendment Agency: Food and Drug Administration, HHS. ACTION: Final rule; technical amendment. SUMMARY: The Food and Drug Administration (FDA) is amending its...
2012 Technical Corrections Fact Sheet
Final Rule: 2012 Technical Corrections, Clarifying and Other Amendments to theGreenhouse Gas Reporting Rule, and Confidentiality Determinations for Certain DataElements of the Fluorinated Gas Source Category
DOE Office of Scientific and Technical Information (OSTI.GOV)
de Boer, Gijs; Lawrence, Dale; Palo, Scott
2017-03-29
This final technical report details activities undertaken as part of the referenced project. Included is information on the preparation of aircraft for deployment to Alaska, summaries of the three deployments covered under this project, and a brief description of the dataset and science directions pursued. Additionally, we provide information on lessons learned, publications, and presentations resulting from this work.
ERIC Educational Resources Information Center
Gardner, David C.; And Others
Volume 1 of the final report on Project HIRE reports the design, development, field-testing, and refining of self-instructional packages to teach entry level technical vocabulary to learning handicapped students mainstreamed in vocational programs. Volume 2, a management handbook, reports the methods and findings concerning development of…
Joint Technical Architecture for Robotic Systems (JTARS)-Final Report
NASA Technical Reports Server (NTRS)
Bradley, Arthur T.; Holloway, Sidney E., III
2006-01-01
This document represents the final report for the Joint Technical Architecture for Robotic Systems (JTARS) project, funded by the Office of Exploration as part of the Intramural Call for Proposals of 2005. The project was prematurely terminated, without review, as part of an agency-wide realignment towards the development of a Crew Exploration Vehicle (CEV) and meeting the near-term goals of lunar exploration.
ERIC Educational Resources Information Center
Bobronnikov, Ellen; Rhodes, Hilary; Bradley, Cay
2010-01-01
This final report culminates the evaluation and technical assistance provided for the U.S. Department of Education's Mathematics and Science Partnership (MSP) Program and its projects since 2005. As part of this support, Abt Associates looked across the portfolio of projects funded by the MSP program to draw lessons on best practices. This…
Pacific Educational Computer Network Study. Final Report.
ERIC Educational Resources Information Center
Hawaii Univ., Honolulu. ALOHA System.
The Pacific Educational Computer Network Feasibility Study examined technical and non-technical aspects of the formation of an international Pacific Area computer network for higher education. The technical study covered the assessment of the feasibility of a packet-switched satellite and radio ground distribution network for data transmission…
Community College Technical Mathematics Project. Final Report.
ERIC Educational Resources Information Center
Self, Samuel L.
The purpose of the research project was to develop an applied or technical mathematics curriculum which would meet the needs of vocational-technical students at the community college level. The research project was divided into three distinct phases: Identifying the mathematical concepts requisite for job-entry competencies in each of the…
7 CFR 614.10 - Appeals before the Farm Service Agency county committee.
Code of Federal Regulations, 2010 CFR
2010-01-01
... section are completed, provide the FSA county committee with a written technical determination in the form... part 780, a participant may appeal a final technical determination or a program decision to the FSA... appeal requests review of the technical determination by the applicable State Conservationist prior to...
Federal Register 2010, 2011, 2012, 2013, 2014
2012-08-08
... intervention and preschool service providers with data on their qualifications, certification, and preparation... Priority; Technical Assistance on State Data Collection, Analysis, and Reporting--National IDEA Technical Assistance Center on Early Childhood Longitudinal Data Systems; Rule #0;#0;Federal Register / Vol. 77, No...
77 FR 18716 - Transportation Security Administration Postal Zip Code Change; Technical Amendment
Federal Register 2010, 2011, 2012, 2013, 2014
2012-03-28
... organizational changes and it has no substantive effect on the public. DATES: Effective March 28, 2012. FOR... No. 1572-9] Transportation Security Administration Postal Zip Code Change; Technical Amendment AGENCY: Transportation Security Administration, DHS. ACTION: Final rule. SUMMARY: This rule is a technical change to...
Lyons, Margaret G; Phalen, Ann G
2014-01-01
Research has failed to demonstrate an optimal flushing solution or frequency for central catheters. In a 2002 study of 50 000 home care patients, catheter dysfunction with loss of patency was the most common complication and occurred in 29% of the peripherally inserted central catheters (PICCs) tracked. With the advent of the Affordable Care Act and the promise of expanded home care services, this study offers evidence as to a preferred flushing protocol to prevent catheter patency complications for home infusion patients with PICCs. This prospective, randomized, 1-way, single-blinded posttest with control group study was performed to compare 3 commonly used flushing protocols in home infusion patients with PICCs. The independent variable was the flushing protocol, and dependent variables included the development of patency-related complications and other significant issues such as sluggishness, occlusion, missed medication doses, catheter replacement, additional nursing visits, and the use of alteplase (Cathflo Activase). Each of the study groups had patients who experienced 1 or more patency-related complications. Additional factors that may affect catheter function, including patient age, gender, diagnosis, therapy type, frequency of catheter use, catheter brand/size/number of lumens, concomitant use of anticoagulant medications, and whether PICCs were used for routine lab testing, were analyzed, and no statistical significance was determined. Catheter dwell time (catheter days) was statistically significant (p = .003, confidence interval = 95%; assuming equal variance) and confirmed the assumption that the longer a home care patient's catheter was in place, the more complications occurred. There were no cases of heparin allergy, heparin-induced thrombocytopenia, or line infection. The data provide some evidence to support the elimination of heparin flushing in home care patients with PICCs, although data in the saline-only group that related to additional registered nurse visits to assess PICC patency and the use of alteplase (Cathflo Activase) were trending toward significance because this group experienced a higher incidence of these complications than both the heparin groups. These findings should not be translated to home care patients with cancer or pregnancy diagnoses because these populations were excluded from this study. More studies of this topic area should be initiated. Please see video abstract, Supplemental Digital Content 1, for more information (http://links.lww.com/JIN/A3).
Studying the Cost and Value of Library Services: Final Report. Technical Report APLAB/94-3/1,2,3,4.
ERIC Educational Resources Information Center
Kantor, Paul B.; And Others
This is the final technical report (in three parts) of a 15-month long project to study the costs and value of library functions at five major research libraries. Twenty-one services or service aspects were studied, and numerous measures of the importance or benefit of the service to the users were made. These measures were studied together to lay…
ERIC Educational Resources Information Center
Zull, Carolyn Gifford, Ed.; And Others
This third volume of the Comparative Systems Laboratory (CSL) Final Technical Report is a collection of relatively independent studies performed on CSL materials. Covered in this document are studies on: (1) properties of files, including a study of the growth rate of a dictionary of index terms as influenced by number of documents in the file and…
ERIC Educational Resources Information Center
Hammond, Cathy; Withington, Cairen; Sharp, Julia L.; Mobley, Catherine; Drew, Sam F.; Stringfield, Samuel C.; Stipanovic, Natalie; Swiger, Caroline M.; Daugherty, Lindsay; Griffith, Cathy
2014-01-01
This final report presents findings from data collection and analysis conducted during a five-year study by the National Dropout Prevention Center (NDPC) at Clemson University, in conjunction with colleagues from the National Research Center for Career and Technical Education (NRCCTE) at the University of Louisville. This project was one of three…
Ground-Based Radiometric Measurements of Slant Path Attenuation in the V/W Bands
2016-04-01
GROUND-BASED RADIOMETRIC MEASUREMENTS OF SLANT PATH ATTENUATION IN THE V/W BANDS APRIL 2016 FINAL TECHNICAL REPORT APPROVED FOR PUBLIC RELEASE...2. REPORT TYPE FINAL TECHNICAL REPORT 3. DATES COVERED (From - To) OCT 2012 – SEP 2015 4. TITLE AND SUBTITLE GROUND-BASED RADIOMETRIC MEASUREMENTS ...SUPPLEMENTARY NOTES 14. ABSTRACT Ground-based radiometric techniques were applied to measure the slant path attenuation cumulative distribution function to
DOE Office of Scientific and Technical Information (OSTI.GOV)
Bender, W.
2013-01-01
Final technical progress report of SunShot Incubator Solaflect Energy. The project succeeded in demonstrating that the Solaflect Suspension Heliostat design is viable for large-scale CSP installations. Canting accuracy is acceptable and is continually improving as Solaflect improves its understanding of this design. Cost reduction initiatives were successful, and there are still many opportunities for further development and further cost reduction.
NASA Technical Reports Server (NTRS)
1995-01-01
The purpose of the Advanced Transportation System Studies (ATSS) Technical Area 2 (TA-2) Heavy Lift Launch Vehicle Development contract was to provide advanced launch vehicle concept definition and analysis to assist NASA in the identification of future launch vehicle requirements. Contracted analysis activities included vehicle sizing and performance analysis, subsystem concept definition, propulsion subsystem definition (foreign and domestic), ground operations and facilities analysis, and life cycle cost estimation. This document is Volume 2 of the final report for the contract. It provides documentation of selected technical results from various TA-2 analysis activities, including a detailed narrative description of the SSTO concept assessment results, a user's guide for the associated SSTO sizing tools, an SSTO turnaround assessment report, an executive summary of the ground operations assessments performed during the first year of the contract, a configuration-independent vehicle health management system requirements report, a copy of all major TA-2 contract presentations, a copy of the FLO launch vehicle final report, and references to Pratt & Whitney's TA-2 sponsored final reports regarding the identification of Russian main propulsion technologies.
Technical Evaluation Motor No. 7 (TEM-7)
NASA Technical Reports Server (NTRS)
Hughes, Phil
1991-01-01
The Technical Evaluation Motor No. 7 (TEM-7) test was a full-scale, full duration static test firing of a high performance motor-configuration solid rocket motor with nozzle vectoring. The final test report documents the procedures, performance, and results of the static test firing of TEM-7. All observations, discussions, conclusions, and recommendations included in the report are complete and final except for the TEM-7 fixed housing unbond investigation. A presentation and discussion of TEM-7 performance, anomalies, and test result concurrence with the objectives outlined in CTP-0107, Rev A, Space Shuttle Technical Evaluation Motor No. 7 (TEM-7) Static Fire Test Plan are included.
Building Stronger State Energy Partnerships with the U.S. Department of Energy
DOE Office of Scientific and Technical Information (OSTI.GOV)
Marks, Kate
2011-09-30
This final technical report details the results of total work efforts and progress made from October 2007 – September 2011 under the National Association of State Energy Officials (NASEO) cooperative agreement DE-FC26-07NT43264, Building Stronger State Energy Partnerships with the U.S. Department of Energy. Major topical project areas in this final report include work efforts in the following areas: Energy Assurance and Critical Infrastructure, State and Regional Technical Assistance, Regional Initiative, Regional Coordination and Technical Assistance, and International Activities in China. All required deliverables have been provided to the National Energy Technology Laboratory and DOE program officials.
Final Technical Report of Project DE-FG02-96ER14647
DOE Office of Scientific and Technical Information (OSTI.GOV)
Lundeen, Stephen R.
This is the final technical report of work completed under DOE support over the period Sept. 1, 1996 until May 31, 2015. The title of the project was "Ion/Excited Atom Collision Studies with a Rydberg Target and a CO2 Laser" from 9/1/96 to 10/31/06, and "Properties of Actinide Ions from Measurements of Rydberg Ion Fine Structure" from 11/1/06 until 5/31/15. The primary technical results were a detailed experimental study of resonant charge transfer between Rydberg atoms and highly-charged ions, and unique measurements of many properties of multiply-charged Thorium ions.
Federal Register 2010, 2011, 2012, 2013, 2014
2013-06-07
...] Guidance for Industry and Food and Drug Administration Staff: Technical Considerations for Pen, Jet, and... availability of a final guidance document entitled ``Technical Considerations for Pen, Jet, and Related... developing information to support a marketing application for a pen, jet, or related injector device intended...
Florida Study of Career and Technical Education. Final Report
ERIC Educational Resources Information Center
Jacobson, Louis; Mokher, Christine
2014-01-01
A key goal of the "Carl D. Perkins Career and Technical Education Act of 2006" ("Perkins IV") is to ensure career and technical education (CTE) programs are widely available for preparing high school and college students for "high skill, high wage, or high demand occupations in current or emerging professions"…
DOE Office of Scientific and Technical Information (OSTI.GOV)
Solares, Santiago D.
The final project report covering the period 7/1/14-6/30/17 provides an overview of the technical accomplishments in the areas of (i) fundamental viscoelasticity, (ii) multifrequency atomic force microscopy, and (iii) characterization of energy-relevant materials with atomic force microscopy. A list of publications supported by the project is also provided.
Application of Knowledge-Based Techniques to Tracking Function
2006-09-01
38394041 42434445 46474849 505152 53545556 57585960 616263 646566 676869 707172 737475 7677 7879 8081 8283 8485 8687 8889 9091 9293 9495 969798 99100...Knowledge-based applications to adaptive space-time processing. Volume I: Summary”, AFRL-SN-TR-2001-146 Vol. I (of Vol. VI ), Final Technical Report, July...2001-146 Vol. IV (of Vol. VI ), Final Technical Report, July 2001. [53] C. Morgan, L. Moyer, “Knowledge-based applications to adaptive space-time
ERIC Educational Resources Information Center
Powers, Thomas F., Ed.; Swinton, John R., Ed.
This third and final volume of a study on the future of the food service industry contains the technical papers on which the information in the previous two volumes was based. The papers were written by various members of the Pennsylvania State University departments of economics, food science, nutrition, social psychology, and engineering and by…
Final Technical Report for contract number DE-FG02-05ER15670
DOE Office of Scientific and Technical Information (OSTI.GOV)
Glazebrook, Jane
This is the final technical report for contract number DE-FG02-05ER15670. The project is now complete, and results of the project have been published. Two papers were published based on work done in the last three-year funding period. The DOIs of these papers are included below. The abstracts of the papers, providing summaries of the work, are included in the body of the report.
Astrobee Periodic Technical Review (PTR) Delta 3
NASA Technical Reports Server (NTRS)
Provencher, Christopher; Smith, Marion F.; Smith, Ernest Everett; Bualat, Maria Gabriele; Barlow, Jonathan Spencer
2017-01-01
Astrobee is a free flying robot for the inside of the International Space Station (ISS). The Periodic Technical Review (PTR) delta 3 is the final design review of the system presented to stakeholders.
Polar source analysis : technical memorandum
DOT National Transportation Integrated Search
2017-09-29
The following technical memorandum describes the development, testing and analysis of various polar source data sets. The memorandum also includes recommendation for potential inclusion in future releases of AEDT. This memorandum is the final deliver...
2005 v4.2 Technical Support Document
Technical Support Document for the Final Transport Rule describes how updated 2005 NEI, version 2 emissions and were processed for air quality modeling in support of the Cross-state Air Pollution Rule (CSAPR).
Direct Final Rule for Technical Amendments for Marine Spark-Ignition Engines and Vessels
Rule published September 16, 2010 to make technical amendments to the design standard for portable marine fuel tanks. This rule incorporates safe recommended practices, developed through industry consensus.
Seattle To Portland Inter-City ITS Corridor Study And Communications Plan, Final Report
DOT National Transportation Integrated Search
1996-03-01
THIS DOCUMENT IS THE FINAL REPORT PRESENTING THE SEATTLE TO PORTLAND INTELLIGENT TRANSPORTATION SYSTEM (ITS) EARLY DEPLOYMENT PLAN. THE FINAL REPORT SYNTHESIZES INFORMATION FROM TECHNICAL MEMORANDUMS 1 THROUGH 5; INCLUDING EXISTING AND FUTURE CONDITI...
Benchmark Analysis of Career and Technical Education in Lenawee County. Final Report.
ERIC Educational Resources Information Center
Hollenbeck, Kevin
The career and technical education (CTE) provided in grades K-12 in the county's vocational-technical center and 12 local public school districts of Lenawee County, Michigan, was benchmarked with respect to its attention to career development. Data were collected from the following sources: structured interviews with a number of key respondents…
ERIC Educational Resources Information Center
United Nations Educational, Scientific, and Cultural Organization, Paris (France).
The international advisory committee of the International Project on Technical and Vocational Education (UNEVOC) held its third session in Paris in October 1995. Advisory committee members and observers from the United Nations' specialized, intergovernmental, and nongovernmental organizations reviewed the major UNEVOC project activities undertaken…
ERIC Educational Resources Information Center
United Nations Educational, Scientific, and Cultural Organization, Paris (France).
The international advisory committee of the International Project on Technical and Vocational Education (UNEVOC) held its second session in Paris in December 1994. Nine advisory committee members and observers from the United Nations' specialized, intergovernmental, and nongovernmental organizations reviewed the major UNEVOC project activities…
Final Technical Report for ARRA Funding
DOE Office of Scientific and Technical Information (OSTI.GOV)
Rusack, Roger; Mans, Jeremiah; Poling, Ronald
Final technical report of the University of Minnesota experimental high energy physics group for ARRA support. The Cryogenic Dark Matter Experiment (CDMS) used the funds received to construct a new passive shield to protect a high-purity germanium detector located in the Soudan mine in Northern Minnesota from cosmic rays. The BESIII and the CMS groups purchased computing hardware to assemble computer farms for data analysis and to generate large volumes of simulated data for comparison with the data collected.
ERIC Educational Resources Information Center
Love, John M.; Kisker, Ellen Eliason; Ross, Christine M.; Schochet, Peter Z.; Brooks-Gunn, Jeanne; Paulsell, Diane; Boller, Kimberly; Constantine, Jill; Vogel, Cheri; Fuligni, Alison Sidle; Brady-Smith, Christy
Early Head Start was designed in 1994 as a 2-generation program to enhance children's development and health, strengthen family and community partnerships, and support the staff delivering new services to low-income families with pregnant women, infants, or toddlers. This document contains the final technical report, appendixes, and local…
ERIC Educational Resources Information Center
United Nations Educational, Scientific, and Cultural Organization, Paris (France).
The international advisory committee of the International Project on Technical and Vocational Education (UNEVOC) held its first session in Berlin in September 1993. The advisory committee's 10 members and observers from the United Nations' specialized, intergovernmental, and nongovernmental organizations discussed educational policy, links between…
DOE Office of Scientific and Technical Information (OSTI.GOV)
NONE
2005-11-08
This final technical report contains the abstracts and executive summaries of projects funded through the Illinois Clean Coal Institute solicitation entitled 'Request for proposals No. 04-1(ICCI/RFP04-1)'. Support of these projects is by the Office of Coal Development and Department of Commerce and Economic Opportunity. The projects fall into the following categories: advanced coal mining technologies; coal preparation and coal production business practice; management of coal combustion byproducts; commercialization and technology transfer. Final project extensions are also recorded.
Renewable Fuel Standard (RFS2): Final Rule Additional Resources
The final rule of fuels and fuel additives: renewable fuel standard program is published on March 26, 2010 and is effective on July 1, 2010. You will find the links to this final rule and technical amendments supporting this rule.
75 FR 48273 - Technical Service Provider Assistance
Federal Register 2010, 2011, 2012, 2013, 2014
2010-08-10
... DEPARTMENT OF AGRICULTURE Natural Resources Conservation Service 7 CFR Part 652 RIN 0578-AA48 Technical Service Provider Assistance AGENCY: Natural Resources Conservation Service, United States Department of Agriculture. ACTION: Final rule; Correcting amendment. SUMMARY: The Natural Resources...
Partial Withdrawal and Final Rule for Nonroad Technical Amendments
Amendments to the technical hardship provisions under the Transition Program for Equipment Manufacturers related to the Tier 4 standards for nonroad diesel engines, and to the replacement engine exemption generally applicable to new nonroad engines.
ERIC Educational Resources Information Center
Norwich Regional Vocational Technical School, CT.
Responding to a Small Business Administration statement that technical school graduates lack knowledge of business principles, the Norwich (Connecticut) Regional Vocational Technical School conducted a course in small business ownership/management for all of its seniors. The required course, a fifty-four-hour module, replaced one English,…
ERIC Educational Resources Information Center
Behr, Shirley K.; And Others
The report describes the third and final year of a 3-year case study of the technical assistance process as implemented by the Technical Assistance Development System (TADS) for the staffs of two demonstration programs for preschool handicapped children and their families. Following a review of TADS and the two demonstration programs, the…
Chemical Durability Improvement and Static Fatigue of Glasses.
1982-08-01
Afl-Alla 837 RENSSELAER POLYIECmfJ!C INST TRtOY NY DEPT OF MATERIAL--ETC F/6 ii/ CHEMICAL DURABILITY IMPROVEMENT AND STATIC FATIGUE OF GLASSESW AUC2...82 M TOMOZAWA NOGGIN 7A-C-0315 UNC LASS IF IED N ENEEEEEE FINAL TECHNICAL REPORT For the period April 1, 1978 "u March 31, 198200 CHEMICAL DURABILITY...REPORT A PERIOD COVERED Chemical Durability Improvement and Static Final Technical Report Fatiue o GlasesApril 1, 1978"’,March 31, 1982 S. PERFORMING ORG
Experimental Program Final Technical Progress Report: 15 February 2007 to 30 September 2012
DOE Office of Scientific and Technical Information (OSTI.GOV)
Kinney, Edward R.
2014-09-12
This is the final technical report of the grant DE-FG02-04ER41301 to the University of Colorado at Boulder entitled "Intermediate Energy Nuclear Physics" and describes the results of our funded activities during the period 15 February 2007 to 30 September 2012. These activities were primarily carried out at Fermilab, RHIC, and the German lab DESY. Significant advances in these experiments were carried out by members of the Colorado group and are described in detail.
Final Technical Report - Center for Technology for Advanced Scientific Component Software (TASCS)
DOE Office of Scientific and Technical Information (OSTI.GOV)
Sussman, Alan
2014-10-21
This is a final technical report for the University of Maryland work in the SciDAC Center for Technology for Advanced Scientific Component Software (TASCS). The Maryland work focused on software tools for coupling parallel software components built using the Common Component Architecture (CCA) APIs. Those tools are based on the Maryland InterComm software framework that has been used in multiple computational science applications to build large-scale simulations of complex physical systems that employ multiple separately developed codes.
Iowa Hill Pumped Storage Project Investigations - Final Technical Report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Hanson, David
2016-07-01
This Final Technical Report is a summary of the activities and outcome of the Department of Energy (DOE) Assistance Agreement DE-EE0005414 with the Sacramento Municipal Utility District (SMUD). The Assistance Agreement was created in 2012 to support investigations into the Iowa Hill Pumped-storage Project (Project), a new development that would add an additional 400 MW of capacity to SMUD’s existing 688MW Upper American River Hydroelectric Project (UARP) in the Sierra Nevada mountains east of Sacramento, California.
Renewable Fuel Standard Program (RFS1): Final Rule Additional Resources
The final rule of fuels and fuel additives: renewable fuel standard program is published on May 1, 2007 and is effective on September 1, 2007. You will find the links to this final rule and technical amendments supporting this rule.
Assurance Penalty Level Analysis Final Rule TSD
This Technical Support Document (TSD) supports EPA’s determination that the final Transport Rule’s assurance provision penalty requirement provides sufficient deterrence against a state exceeding its assurance level.
Final Rule for Technical Amendments to the Highway and Nonroad Diesel Regulations
This action corrects errors and omissions from the previous rules, makes minor changes to the regulations to assist entities with regulatory compliance, and makes technical amendments that resulted from discussions with various diesel stakeholders.
2014-08-19
The Assistant Secretary for Special Education and Rehabilitative Services announces a priority under the Rehabilitation Training program to establish a Job-Driven Vocational Rehabilitation Technical Assistance Center (JDVRTAC). The Assistant Secretary may use this priority for competitions in fiscal year (FY) 2014 and later years. We take this action to focus on training in an area of national need. Specifically, this priority responds to the Presidential Memorandum to Federal agencies directing them to take action to address job-driven training for the Nation's workers. The JDVRTAC will provide technical assistance (TA) to State vocational rehabilitation (VR) agencies to help them develop for individuals with disabilities training and employment opportunities that meet the needs of today's employers.
From chaperonins to Rubisco assembly and metabolic repair.
Hayer-Hartl, Manajit
2017-12-01
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) mediates the fixation of atmospheric CO 2 in photosynthesis by catalyzing the carboxylation of the 5-carbon sugar ribulose-1,5-bisphosphate (RuBP). Despite its pivotal role, Rubisco is an inefficient enzyme and thus has been a key target for bioengineering. However, efforts to increase crop yields by Rubisco engineering remain unsuccessful, due in part to the complex machinery of molecular chaperones required for Rubisco biogenesis and metabolic repair. While the large subunit of Rubisco generally requires the chaperonin system for folding, the evolution of the hexadecameric Rubisco from its dimeric precursor resulted in the dependence on an array of additional factors required for assembly. Moreover, Rubisco function can be inhibited by a range of sugar-phosphate ligands. Metabolic repair of Rubisco depends on remodeling by the ATP-dependent Rubisco activase and hydrolysis of inhibitors by specific phosphatases. This review highlights our work toward understanding the structure and mechanism of these auxiliary machineries. © 2017 The Protein Society.
Biogenesis and Metabolic Maintenance of Rubisco.
Bracher, Andreas; Whitney, Spencer M; Hartl, F Ulrich; Hayer-Hartl, Manajit
2017-04-28
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) mediates the fixation of atmospheric CO 2 in photosynthesis by catalyzing the carboxylation of the 5-carbon sugar ribulose-1,5-bisphosphate (RuBP). Rubisco is a remarkably inefficient enzyme, fixing only 2-10 CO 2 molecules per second. Efforts to increase crop yields by bioengineering Rubisco remain unsuccessful, owing in part to the complex cellular machinery required for Rubisco biogenesis and metabolic maintenance. The large subunit of Rubisco requires the chaperonin system for folding, and recent studies have shown that assembly of hexadecameric Rubisco is mediated by specific assembly chaperones. Moreover, Rubisco function can be inhibited by a range of sugar-phosphate ligands, including RuBP. Metabolic repair depends on remodeling of Rubisco by the ATP-dependent Rubisco activase and hydrolysis of inhibitory sugar phosphates by specific phosphatases. Here, we review our present understanding of the structure and function of these auxiliary factors and their utilization in efforts to engineer more catalytically efficient Rubisco enzymes.
NASA's Pursuit of Low-Noise Propulsion for Low-Boom Commercial Supersonic Vehicles
NASA Technical Reports Server (NTRS)
Bridges, James; Brown, Clifford A.; Seidel, Jonathan A.
2018-01-01
Since 2006, when the Fundamental Aeronautics Program was instituted within NASA's Aeronautics Mission Directorate, there has been a Project looking at the technical barriers to commercial supersonic flight. Among the barriers is the noise produced by aircraft during landing and takeoff. Over the years that followed, research was carried out at NASA aeronautics research centers, often in collaboration with academia and industry, addressing the problem. In 2013, a high-level milestone was established, described as a Technical Challenge, with the objective of demonstrating the feasibility of a low-boom supersonic airliner that could meet current airport noise regulations. The Technical Challenge was formally called "Low Noise Propulsion for Low Boom Aircraft", and was completed in late 2016. This paper reports the technical findings from this Technical Challenge, reaching back almost 10 years to review the technologies and tools that were developed along the way. It also discusses the final aircraft configuration and propulsion systems required for a supersonic civilian aircraft to meet noise regulations using the technologies available today. Finally, the paper documents the model-scale tests that validated the acoustic performance of the study aircraft.
NASA's Pursuit of Low-Noise Propulsion for Low-Boom Commercial Supersonic Vehicles
NASA Technical Reports Server (NTRS)
Bridges, James; Brown, Clifford A.; Seidel, Jonathan
2018-01-01
Since 2006, when the Fundamental Aeronautics Program was instituted within NASA's Aeronautics Mission Directorate, there has been a Project looking at the technical barriers to commercial supersonic flight. Among the barriers is the noise produced by aircraft during landing and takeoff. Over the years that followed, research was carried out at NASA aeronautics research centers, often in collaboration with academia and industry, addressing the problem. In 2013, a high-level milestone was established, described as a Technical Challenge, with the objective of demonstrating the feasibility of a low-boom supersonic airliner that could meet current airport noise regulations. The Technical Challenge was formally called a Low Noise Propulsion for Low Boom Aircraft and was completed in late 2016. This paper reports the technical findings from this Technical Challenge, reaching back almost 10 years to review the technologies and tools that were developed along the way. It also discusses the final aircraft configuration and propulsion systems required for a supersonic civilian aircraft to meet noise regulations using the technologies available today. Finally, the paper documents the model-scale tests that validated the acoustic performance of the study aircraft.
Final Revisions Rule State Budgets and New Unit Set-Asides TSD
This technical support document shows the underlying data and calculations used to quantify the state budget revisions and new unit set-aside revisions made in the final revisions rule, as well as those revisions included in the direct final revisions rule
77 FR 37284 - Technical Amendments
Federal Register 2010, 2011, 2012, 2013, 2014
2012-06-21
... DEPARTMENT OF LABOR Office of Workers' Compensation Programs 20 CFR Parts 701, 702, 703, 725, and 726 RIN 1240-AA05 Technical Amendments AGENCY: Office of Workers' Compensation Programs, Labor. ACTION: Final rule. SUMMARY: The Office of Workers' Compensation Programs is making [[Page 37285
Vehicle infrastructure integration proof of concept : technical description--vehicle : final report
DOT National Transportation Integrated Search
2009-05-19
This report provides the technical description of the VII system developed for the Cooperative Agreement VII Program between the USDOT and the VII Consortium. The basic architectural elements are summarized and detailed descriptions of the hardware a...
NASA Technical Reports Server (NTRS)
1993-01-01
This is the Final Technical Report for the NetView Technical Research task. This report is prepared in accordance with Contract Data Requirements List (CDRL) item A002. NetView assistance was provided and details are presented under the following headings: NetView Management Systems (NMS) project tasks; WBAFB IBM 3090; WPAFB AMDAHL; WPAFB IBM 3084; Hill AFB; McClellan AFB AMDAHL; McClellan AFB IBM 3090; and Warner-Robins AFB.
DOT National Transportation Integrated Search
2016-12-29
Two project objectives one technical and one educational- were laid out in this project. The technical objective was to assess current inventory of greenhouse gases (GHG) in the six Midwestern states of the nation and to estimate improvements as ...
Emissions Inventory Final Rule TSD
This technical support document (TSD) provides the details of emissions data processing done in support of EPA's final rulemaking effort for the Federal Transport Rule, now known as the Cross-State Air Pollution Rule.
DOT National Transportation Integrated Search
2017-11-01
This report is one of two NCST Research Report documents produced as part of a project to advance the technical modeling tools for resiliency and adaptation planning, especially those used for criticality rankings. The official final technical report...
Comment Deadlines Established Regarding the LightSquared Technical Working Group Report
DOT National Transportation Integrated Search
2011-06-30
On June 30, 2011, LightSquared Subsidiary LLC (LightSquared) submitted a final report of the : technical working group co-chaired by LightSquared and the United States Global Positioning System : (GPS) Industry Council (USGIC)1 and organized in respo...
DOT National Transportation Integrated Search
2015-06-01
This Technical Report on Prototype Intelligent Network Flow Optimization (INFLO) Dynamic Speed Harmonization and Queue Warning is the final report for the project. It describes the prototyping, acceptance testing and small-scale demonstration of the ...
NUSC Technical Publications Guide.
1985-05-01
Facility personnel especially that of A. Castelluzzo, E. Deland, J. Gesel , and E. Szlosek (all of Code 4343). Reviewed and Approved: 14 July 1980 D...their technical content and format. Review and approve the manual outline, the review manuscript, and the final camera - reproducible copy. Conduct in
Cross-State Air Pollution Rule Update Allowance Allocation Final Rule TSD
This Technical Support Document (TSD) provides information that supports EPA’s determination of unit-level allocations for existing and new units under the final Cross-State Air Pollution Rule Update.
Guidelines for producing training films and videos.
Harper, P B
1991-01-01
Drawing from experience in producing a film on the surgical procedure of female sterilization, 4 guidelines to technical film production for training purposes are presented and discussed in this paper. In order of presentation in the text, the paper 1st encourages identifying and securing a technical expert, then clearly identifying steps of the technical procedure, involving trainees and trainers in the production process, and working with experienced producers, scriptwriters, and crew members. Returning to the 1st guideline, the technical advisor will have a central presence during all photography and editing, and ideally should not have any personal investment in the procedure being shown. Prior to script finalization and sorting, research is urged to ensure concrete procedural steps. Printed materials, slides, interviews of experienced clinicians, procedure observation, and test videotape shooting may be called upon and employed as parts of the research phase. Trainees should participate during preliminary research, script development, and pretesting of early film versions, their suggestions for change incorporated where appropriate in the final version. On the final point of securing experienced workers, country nationals sensitive to relevant cultural and background dynamics should be included in the team. The special concerns of airport security regulation and customs requirements knowledge are essential, as well as their attention to assuring adequate on-site electricity for camera equipment.
DOT National Transportation Integrated Search
1994-10-31
This document is the final environmental impact statement and final environmental impact report (FEIS/R) on the proposal by the National Railroad Passenger Corporation (Amtrak) to complete the electrification of the Northeast Corridor main line by ex...
2004-09-21
The rule finalizes technical changes to the Healthcare Integrity and Protection Data Bank (HIPDB) data collection reporting requirements by clarifying the types of personal numeric identifiers that may be reported to the data bank in connection with adverse actions. The rule clarifies that in lieu of a Social Security Number (SSN), an individual taxpayer identification number (ITIN) may be reported to the data bank when, in those limited situations, an individual does not have an SSN.
Recovery Act-SmartGrid regional demonstration transmission and distribution (T&D) Infrastructure
DOE Office of Scientific and Technical Information (OSTI.GOV)
Hedges, Edward T.
This document represents the Final Technical Report for the Kansas City Power & Light Company (KCP&L) Green Impact Zone SmartGrid Demonstration Project (SGDP). The KCP&L project is partially funded by Department of Energy (DOE) Regional Smart Grid Demonstration Project cooperative agreement DE-OE0000221 in the Transmission and Distribution Infrastructure application area. This Final Technical Report summarizes the KCP&L SGDP as of April 30, 2015 and includes summaries of the project design, implementation, operations, and analysis performed as of that date.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Sobecky, Patricia A; Taillefert, Martial
This final technical report describes results and findings from a research project to examine the role of microbial phosphohydrolase enzymes in naturally occurring subsurface microorganisms for the purpose of promoting the immobilization of the radionuclide uranium through the production of insoluble uranium phosphate minerals. The research project investigated the microbial mechanisms and the physical and chemical processes promoting uranium biomineralization and sequestration in oxygenated subsurface soils. Uranium biomineralization under aerobic conditions can provide a secondary biobarrier strategy to immobilize radionuclides should the metal precipitates formed by microbial dissimilatory mechanisms remobilize due to a change in redox state.
Waveguide Studies for Fiber Optics and Optical Signal Processing Applications.
1980-04-01
AO-A086 115 UNI!VERtSIT? OF SOUTIUR CALEPCRNA LOS AMUSS / 5 WAVGUIDE STUIES15 FOR FEB53 OpTECS AND OpTICAL SEOSA.o P /0Ksu-y "/6 UNLSIIDAPR N0 E...SAMUE Flola-??-c-sa UNCASZFIORAC-M-8042 U Final Technical Report (1 1April 1950 L V ~ WAVEGUIDE STUDIES FOR FIBER OPTICS AND OPTICAL SIGNAL PROCESSING...and Subtitle) 081 6&4JODO )EI YAVECUIDESTUDIES FOR JIBER OPTICS ANDL 7 Final ,T/echnical epoErt, OPTICAL SI’tNAL PROCESSING APPLICATIONS.4 11 Se 77
ERIC Educational Resources Information Center
BIVONA, WILLIAM A.
THIS VOLUME PRESENTS THE RESULTS OF A NINE-MONTH TEST OF A PROTOTYPE SELECTIVE DISSEMINATION OF INFORMATION (SDI) SYSTEM DEVELOPED FOR THE ARMY TECHNICAL LIBRARIES. DURING THE PILOT TEST ONE THOUSAND DOCUMENTS WERE CATALOGED, INDEXED, AND DISSEMINATED TO TWENTY-FIVE SCIENTIFIC AND TECHNICAL PERSONNEL. MATCHING OF THE INTEREST PROFILES OF THESE…
Ceramic High Efficiency Particulate Air (HEPA) Filter Final Report CRADA No. TC02160.0
DOE Office of Scientific and Technical Information (OSTI.GOV)
Mitchell, M.; Bergman, W.
2017-08-25
The technical objective of this project was to develop a ceramic HEPA filter technology, by initially producing and testing coupon ceramics, small scale prototypes, and full scale prototype HEPA filters, and to address relevant manufacturing and commercialization technical issues.
7 CFR 614.6 - Agency records and decision notices.
Code of Federal Regulations, 2010 CFR
2010-01-01
... notifies participants of the agency's preliminary and final technical determinations and program decisions... decision notice within 10 working days of rendering a technical determination or program decision. In lieu of certified mail, NRCS may hand deliver notices to participants with written acknowledgment of...
ECOS E-MATRIX Methane and Volatile Organic Carbon (VOC) Emissions Best Practices Database
DOE Office of Scientific and Technical Information (OSTI.GOV)
Parisien, Lia
2016-01-31
This final scientific/technical report on the ECOS e-MATRIX Methane and Volatile Organic Carbon (VOC) Emissions Best Practices Database provides a disclaimer and acknowledgement, table of contents, executive summary, description of project activities, and briefing/technical presentation link.
77 FR 18914 - National Motor Vehicle Title Information System (NMVTIS): Technical Corrections
Federal Register 2010, 2011, 2012, 2013, 2014
2012-03-29
... 1121-AA79 National Motor Vehicle Title Information System (NMVTIS): Technical Corrections AGENCY... (OJP) is promulgating this direct final rule for its National Motor Vehicle Title Information System... INFORMATION CONTACT paragraph. II. Background The National Motor Vehicle Title Information System was...
DOT National Transportation Integrated Search
1997-06-18
This document provides a technical summary for the seven working papers prepared for the New York State Department of Transportation (NYSDOT) Buffalo and Niagara Falls Intelligent Transportation System (ITS) Study.
77 FR 8095 - Technical Corrections to Commission Regulations
Federal Register 2010, 2011, 2012, 2013, 2014
2012-02-14
... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission 18 CFR Part 2 [Docket No. RM11-30-000; Order No. 756] Technical Corrections to Commission Regulations Issued February 8, 2012. AGENCY: Federal Energy Regulatory Commission, DOE. ACTION: Final rule: correcting amendment. SUMMARY: This document adds...
78 FR 13543 - Defense Federal Acquisition Regulation Supplement; Technical Amendments
Federal Register 2010, 2011, 2012, 2013, 2014
2013-02-28
... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Parts 201, 204, 215, 225, 227, 242, 245, and 252 Defense Federal Acquisition Regulation Supplement; Technical Amendments AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Final rule. SUMMARY: DoD is...
Alternatives for Jet Engine Control. Volume 1: Modelling and Control Design with Jet Engine Data
NASA Technical Reports Server (NTRS)
Sain, M. K.
1985-01-01
This document compiles a comprehensive list of publications supported by, or related to, National Aeronautics and Space Administration Grant NSG-3048, entitled "Alternatives for Jet Engine Control". Dr. Kurt Seldner was the original Technical Officer for the grant, at Lewis Research Center. Dr. Bruce Lehtinen was the final Technical Officer. At the University of Notre Dame, Drs. Michael K. Sain and R. Jeffrey Leake were the original Project Directors, with Dr. Sain becoming the final Project Director. Publications cover work over a ten-year period. The Final Report is divided into two parts. Volume i, "Modelling and Control Design with Jet Engine Data", follows in this report. Volume 2, "Modelling and Control Design with Tensors", has been bound separately.
48 CFR 252.235-7011 - Final scientific or technical report.
Code of Federal Regulations, 2014 CFR
2014-10-01
... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...
48 CFR 252.235-7011 - Final scientific or technical report.
Code of Federal Regulations, 2012 CFR
2012-10-01
... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...
48 CFR 252.235-7011 - Final scientific or technical report.
Code of Federal Regulations, 2010 CFR
2010-10-01
... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...
48 CFR 252.235-7011 - Final scientific or technical report.
Code of Federal Regulations, 2011 CFR
2011-10-01
... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...
48 CFR 252.235-7011 - Final scientific or technical report.
Code of Federal Regulations, 2013 CFR
2013-10-01
... technical report. 252.235-7011 Section 252.235-7011 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT... of the report; and (c) For submission of reports in other than paper copy, contact the Defense...
Vocational Outreach Involving Community Exchange (VOICE). Final Report.
ERIC Educational Resources Information Center
Huckabee, Johnni
A Jonesboro, Arkansas project was designed and implemented to increase the awareness of vocational education, increase community support and involvement in vocational education, and establish improved communication in the vocational-technical education field. Interaction between the vocational-technical school and the local school community was a…
King County Metro Transit Hybrid Articulated Buses: Final Evaluation Results
DOE Office of Scientific and Technical Information (OSTI.GOV)
Chandler, K.; Walkowicz, K.
2006-12-01
Final technical report compares and evaluates new diesel and diesel hybrid-electric articulated buses operated as part of the King County Metro Transit (KC Metro) fleet in Seattle, Washington. The evaluation lasted 12 months.
78 FR 1143 - Explosive Siting Requirements; Correction
Federal Register 2010, 2011, 2012, 2013, 2014
2013-01-08
... launch site operators in site planning for the storage and handling of energetic liquids and explosives...: For technical questions concerning this final rule, contact Yvonne Tran, Commercial Space... this final rule, contact Laura Montgomery, AGC 200, [[Page 1144
Final June Revisions Rule State Budgets and New Unit Set-Asides TSD
This technical support document (TSD) for the final revisions to the Transport Rule shows the underlying data and calculations used to quantify the state budget revisions and new unit set-aside revisions.
Department of Defense Access to Intellectual Property for Weapon Systems Sustainment
2017-05-01
and acquiring technical data rights … The cost benefit analysis of including a priced contract option for the future delivery of technical data...entail in terms of cost and benefits , while one of the activities to be finalized is the contract-specific technical data elements.66...Virginia 22311-1882 May 2017 Approved for public release; distribution is unlimited. IDA Paper P-8266 Log: H 17-000030 About This Publication This
10 CFR 51.92 - Supplement to the final environmental impact statement.
Code of Federal Regulations, 2011 CFR
2011-01-01
... changes in the proposed action that are relevant to environmental concerns; or (2) There are new and..., technical, and other benefits and costs of the proposed action, to the extent that the final environmental... costs; (5) Include an analysis of other energy alternatives, to the extent that the final environmental...
ERIC Educational Resources Information Center
Munushian, Jack
In 1972, the University of Southern California School of Engineering established a 4-channel interactive instructional television network. It was designed to allow employees of participating industries to take regular university science and engineering courses and special continuing education courses at or near their work locations. Final progress…
The role of non-technical skills in surgery
Agha, Riaz A.; Fowler, Alexander J.; Sevdalis, Nick
2015-01-01
Non-technical skills are of increasing importance in surgery and surgical training. A traditional focus on technical skills acquisition and competence is no longer enough for the delivery of a modern, safe surgical practice. This review discusses the importance of non-technical skills and the values that underpin successful modern surgical practice. This narrative review used a number of sources including written and online, there was no specific search strategy of defined databases. Modern surgical practice requires; technical and non-technical skills, evidence-based practice, an emphasis on lifelong learning, monitoring of outcomes and a supportive institutional and health service framework. Finally these requirements need to be combined with a number of personal and professional values including integrity, professionalism and compassionate, patient-centred care. PMID:26904193
75 FR 16345 - Administrative Practices and Procedures; Good Guidance Practices; Technical Amendment
Federal Register 2010, 2011, 2012, 2013, 2014
2010-04-01
.... FDA-1999-N-3539] (formerly Docket No. 1999N-4783) Administrative Practices and Procedures; Good Guidance Practices; Technical Amendment AGENCY: Food and Drug Administration, HHS. ACTION: Final rule... Subjects in 21 CFR Part 10 Administrative practice and procedure, News media. 0 Therefore, under the...
DOT National Transportation Integrated Search
2000-01-01
The following progress report is intended to highlight the significant activities of the Florida Transit Training Program and Florida Technical Assistant Program. The following progress report is intended to highlight the significant activities of th...
78 FR 34264 - Technical Corrections to the HIPAA Privacy, Security, and Enforcement Rules
Federal Register 2010, 2011, 2012, 2013, 2014
2013-06-07
...-AA03 Technical Corrections to the HIPAA Privacy, Security, and Enforcement Rules AGENCY: Office for... corrections address certain inadvertent errors and omissions in the HIPAA Privacy, Security, and Enforcement... (HHS or ``the Department'') published a final rule to implement changes to the HIPAA Privacy, Security...
Federal Register 2010, 2011, 2012, 2013, 2014
2011-03-02
... Acquisition Regulation Supplement; Independent Research and Development Technical Descriptions (DFARS Case... (DFARS) to require contractors to report independent research and development (IR&D) projects generating... the address shown below on or before May 2, 2011, to be considered in the formation of the final rule...
Professional Personnel Evaluation System: 1983-84 Final Technical Report.
ERIC Educational Resources Information Center
Doss, David A.
This technical report summarizes the evaluation ratings given to professionals on probation or up for contract renewal in the Austin Independent School District. Graphs, tables, and rankings are presented for each specified population: all district teachers combined; teachers by school level (elementary, junior high, senior high); teachers by…
High School Graduation Minimum Competency Requirements. Final Technical Report.
ERIC Educational Resources Information Center
Austin Independent School District, TX. Office of Research and Evaluation.
This technical report details the testing results and analyses supporting the evaluation findings related to the Austin (Texas) Independent School District (AISD) minimum competency graduation requirements. The graduation competency status of all AISD students in grades 8 to 12 are documented. The report provides additional information on the data…
76 FR 16531 - Technical Correction for Neurological Listing Cross-Reference
Federal Register 2010, 2011, 2012, 2013, 2014
2011-03-24
... 1-800-325-0778, or visit our Internet site, Social Security Online, at http://www.socialsecurity.gov... SOCIAL SECURITY ADMINISTRATION 20 CFR Part 404 [Docket No. SSA-2011-0019] RIN 0960-AH33 Technical Correction for Neurological Listing Cross-Reference AGENCY: Social Security Administration. ACTION: Final...
Improving Vocational Education in Post-High School Institutions. Final Report.
ERIC Educational Resources Information Center
MacArthur, Earl W.
Four ongoing programs in postsecondary vocational-technical education were examined in a national institute attended by 59 representatives from 31 states. Institutions reporting programs were: (1) Los Angeles Trade and Technical College, California, (2) Washtenaw Community College, Ann Arbor, Michigan, (3) Rockingham Community College, Wentworth,…
Chicago Manufacturing Tech Prep. Fiscal Year 1991 Final Report.
ERIC Educational Resources Information Center
Chicago City Colleges, IL.
During its first year of development in 1991, the Chicago Manufacturing Technical Preparation (Tech Prep) Program established a plan for implementing an industry-driven, articulated 4-year manufacturing technology course of study that integrates applied academic courses with technical courses and meets industry hiring standards. The project…
Federal Register 2010, 2011, 2012, 2013, 2014
2013-05-20
... reporting requirements. Changes: None. Focus TA on Assessment and Discipline Data Comment: Three commenters agreed with the importance of focusing on assessment and discipline data, and two commenters agreed with... decisions and actions associated with data collection and reporting. One commenter stated that assessment...
Modular Radioisotope Thermoelectric Generator (RTG) Program. Final technical report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Not Available
1992-12-31
Section 2.0 of this report summarizes the MOD-RTG reference flight design, and Section 3.0 discusses the Ground Demonstration System design. Multicouple technology development is discussed in Section 4.0, and Section 5.0 lists all published technical papers prepared during the course of the contract.
ERIC Educational Resources Information Center
Bhattacharyya, Ena; Patil, Arun; Sargunan, Rajeswary Appacutty
2010-01-01
Engineering communication studies indicate the importance of oral presentations as an indispensable component of workplace oral communication activities; however, since there is limited literature regarding stakeholder perceptions of effective presentation skills and attributes in technical oral presentations or final year engineering project…
EPA is establishing or revising initial area designations and a technical amendment to correct an inadvertent error in the initial designation for one area for the 2012 annual national ambient air quality standards for fine particle pollution.
75 FR 41404 - List of Approved Spent Fuel Storage Casks: NUHOMS®
Federal Register 2010, 2011, 2012, 2013, 2014
2010-07-16
.... The NRC is taking this action because the applicant identified that a certain Technical Specification (TS) for Boral characterization was not written precisely. Specifically, the requirements for meeting... changes to the technical specifications. The NRC also published a direct final rule on May 6, 2010 (75 FR...
Research in Automatic Russian-English Scientific and Technical Lexicography. Final Report.
ERIC Educational Resources Information Center
Wayne State Univ., Detroit, MI.
Techniques of reversing English-Russian scientific and technical dictionaries into Russian-English versions through semi-automated compilation are described. Sections on manual and automatic processing discuss pre- and post-editing, the task program, updater (correction of errors and revision by specialist in a given field), the system employed…
ERIC Educational Resources Information Center
System Development Corp., Santa Monica, CA.
A national data program for the marine environment is recommended. Volume 2 includes: (1) objectives, scope, and methodology; (2) summary of the technical development plan; (3) agency development plans - Great Lakes and coastal development and (4) marine data network development plans. (Author)
ERIC Educational Resources Information Center
Pennsylvania State Univ., University Park. Div. of Occupational and Vocational Studies.
Designed for use by area vocational-technical schools and other vocational programs, this project developed courses of study in eight occupational areas: commercial art, appliance repair, automotive mechanics, graphic arts, building trades maintenance, building construction trades, diesel mechanics, and welding. Course-of-study development…
Seminar for Preparation of Professional Personnel for Vocational-Technical Education. Final Report.
ERIC Educational Resources Information Center
Dillon, Roy D.; Horner, James T.
Seminar participants included college administrative officers, state vocational education directors, vocational-technical teacher educators, and Office of Education staff. The purpose of the June, 1968 seminar was to consider strategies for resolving critical vocational education personnel supply and demand problems. Presentations included in the…
75 FR 33167 - Technical Amendment Language Change From “Wholly” to “Fully”
Federal Register 2010, 2011, 2012, 2013, 2014
2010-06-11
... SOCIAL SECURITY ADMINISTRATION 20 CFR Parts 404, 405, 408, 416, and 418 [Docket No. SSA-2009-0062] RIN 0960-AH16 Technical Amendment Language Change From ``Wholly'' to ``Fully'' AGENCY: Social Security... these final rules, call Brian J. Rudick, Office of Regulations, Social Security Administration, 6401...
Federal Register 2010, 2011, 2012, 2013, 2014
2011-09-20
... technical data package, in cases where the Government may have funded only a small portion of the... subcontractor's asserted restrictions on technical data and computer software. DATES: Effective date: September... data and computer software. More specifically, the final rule affects these validation procedures in...
Federal Register 2010, 2011, 2012, 2013, 2014
2010-08-13
... Conforming Amendments, Bridges AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: This rule makes non... technical corrections to Coast Guard bridge and navigable waters regulations. This rule will have no... announces or gathers public opinion or other information regarding bridge matters, nor will it change the...
Research in network management techniques for tactical data communications networks
NASA Astrophysics Data System (ADS)
Boorstyn, R.; Kershenbaum, A.; Maglaris, B.; Sarachik, P.
1982-09-01
This is the final technical report for work performed on network management techniques for tactical data networks. It includes all technical papers that have been published during the control period. Research areas include Packet Network modelling, adaptive network routing, network design algorithms, network design techniques, and local area networks.
ERIC Educational Resources Information Center
Chase, Shirley A.; And Others
A project was conducted to design a system for evaluating microcomputer courseware for vocational and technical education. Through a literature review and contacts with organizations and individuals involved in courseware evaluation and use, project staff identified and acquired for review documents pertaining to courseware evaulation, vocational…
Final Technical Report, Wind Generator Project (Ann Arbor)
DOE Office of Scientific and Technical Information (OSTI.GOV)
Geisler, Nathan
A Final Technical Report (57 pages) describing educational exhibits and devices focused on wind energy, and related outreach activities and programs. Project partnership includes the City of Ann Arbor, MI and the Ann Arbor Hands-on Museum, along with additional sub-recipients, and U.S. Department of Energy/Office of Energy Efficiency and Renewable Energy (EERE). Report relays key milestones and sub-tasks as well as numerous graphics and images of five (5) transportable wind energy demonstration devices and five (5) wind energy exhibits designed and constructed between 2014 and 2016 for transport and use by the Ann Arbor Hands-on Museum.
Final Technical Report- Virginia Solar Pathways Project
DOE Office of Scientific and Technical Information (OSTI.GOV)
Bond, Katharine; Cosby, Sarah
This Report provides a technical review of the final results of a funding award to Virginia Electric and Power Company (Dominion Energy Virginia (DEV) or the Company) for a project under the U.S. Department of Energy’s Solar Energy Technologies Office. The three-year project was formally known as the Virginia Solar Pathways Project (VSPP or the Project). The purpose of the VSPP was to develop a collaborative utility-administered solar strategy (Solar Strategy) for DEV’s service territory in the Commonwealth that could serve as a replicable model for other states with similar policy environments.
1980-12-22
8217AP0A095 772 HENNINGSON DURHAM AND RICHARDSON SANTA BARBARA CA F/G 16/1 B-B ENVIRONMENTAL TECHNICAL REPORT. SOCIOECONOMIC IMPACT ESTIMA--ETC(U) DEC... Environmental Technical Reports,. 53ocioeconctnic’ Final r1eport Impact . Estimates for White Pine County, Nevada. ____________ Detailed Tables 6. PERFORMING...background information for the analysis contained in the M-X Deploment Area Selection and Land Withirawal/Acquisition Draft Environmental Impact Statement
Optical fiber sensors for materials and structures characterization
NASA Technical Reports Server (NTRS)
Lindner, D. K.; Claus, R. O.
1991-01-01
The final technical report on Optical Fiber Sensors for Materials and Structures Characterization, covering the period August 1990 through August 1991 is presented. Research programs in the following technical areas are described; sapphire optical fiber sensors; vibration analysis using two-mode elliptical core fibers and sensors; extrinsic Fabry-Perot interferometer development; and coatings for fluorescent-based sensor. Research progress in each of these areas was substantial, as evidenced by the technical publications which are included as appendices.
A joint university-government technical editing program
NASA Technical Reports Server (NTRS)
Stohrer, Freda F.; Pinelli, Thomas E.
1978-01-01
The NASA Langley Research Center (LaRC) and Old Dominion University have designed a mutually useful technical editing program. A university team made up of an English instructor and two graduate students - one from English, one from engineering - works with a senior editor from LaRC to prepare technical reports for publication. A round-robin technique gives the university team editorial commentary from both language and technical specialists; the senior editor from LaRC supervises reports through final publication. To date, the system has provided LaRC with a respectable product and university students with valuable on-the-job training.
Mature Programs of Study: Examining Policy Implementation at the Local Level. Final Report
ERIC Educational Resources Information Center
Alfeld, Corinne; Bhattacharya, Sharika
2013-01-01
The 2006 Carl D. Perkins Career and Technical Education Act required that all career technical education (CTE) programs offer secondary to postsecondary programs of study (POS), which integrate rigorous academics, offer dual enrollment options, and lead to an industry-recognized degree or credential. Focused on improving students' transition to…
75 FR 37722 - OMB Approvals Under the Paperwork Reduction Act; Technical Amendment
Federal Register 2010, 2011, 2012, 2013, 2014
2010-06-30
... since its last update. B. Why is this Technical Amendment Issued as a Final Rule? The information... provisions since the last update of this table. The paperwork burden associated with these new provisions was... amendment updates the table that lists the Office of Management and Budget (OMB) control numbers issued...
United States Air Force Training Line Simulator. Final Report.
ERIC Educational Resources Information Center
Nauta, Franz; Pierce, Michael B.
This report describes the technical aspects and potential applications of a computer-based model simulating the flow of airmen through basic training and entry-level technical training. The objective of the simulation is to assess the impacts of alternative recruit classification and training policies under a wide variety of assumptions regarding…
Final recovery plan of the southwestern willow flycatcher (Empidonax traillii extimus)
Deborah M. Finch; Stephen I. Rothstein; Jon C. Boren; William L. Graf; Jerry L. Holechek; Barbara E. Kus; Robert M. Marshall; Molly M. Pohl; Susan J. Sferra; Mark K. Sogge; Julie C. Stromberg; Bradley A. Valentine; Mary J. Whitfield; Sartor O. Williams
2002-01-01
The Southwestern Willow Flycatcher Recovery Team is composed of a Technical Subgroup (pg. ii), six Implementation Subgroups (Appendix A), and a Tribal Working Group. The Technical Subgroup consists of 14 academic scientists, researchers, and resource managers with a wide range of expertise in avian biology and ecology, southwestern willow flycatcher ecology, cowbird...
Local/State Bilingual Project. 1981-82 Final Technical Report. Appendixes.
ERIC Educational Resources Information Center
Austin Independent School District, TX. Office of Research and Evaluation.
The 1981-82 Local/State Bilingual Program Technical Report addresses the evaluation questions of the Local/State Bilingual Program Evaluation Design. It is organized into six appendixes. Each appendix reports the information collected by a specific measure. Each appendix consists of (1) an instrument description, (2) purpose of the measure, (3)…
77 FR 26444 - Revisions to Final Response To Petition From New Jersey Regarding SO2
Federal Register 2010, 2011, 2012, 2013, 2014
2012-05-04
... modeling or other technical analyses and no new analyses were necessary to make the revisions. III. Public... this modeling approach. Therefore, no new technical analyses or any changes to the modeling are...) modeling analysis submitted with the September 2010 petition identified NAAQS violations at receptors in...
Federal Register 2010, 2011, 2012, 2013, 2014
2012-07-31
..., are described in the final safety analysis report (FSAR). The staff safety evaluation documents the acceptability of these analyses, and it is the combination of the FSAR analyses and the staff safety evaluation... analysis, maintain their capability to perform their safety functions. Technical Specification Operability...
A Dissemination Model for New Technical Education Programs. Final Report.
ERIC Educational Resources Information Center
Hull, Daniel M.
The Technical Education Research Center-SW has conceived, tested, and refined a model for disseminating newly developed programs and materials throughout the nation. The model performed successfully in the dissemination of more than 50,000 educational units (modules) of Laser/Electro-Optics Technician (LEOT) materials during a four-year period…
Federal Register 2010, 2011, 2012, 2013, 2014
2010-05-21
... Driver's License (CDL); Technical, Organizational, and Conforming Amendments AGENCY: Federal Motor... ``medical examiner's license or certificate number'' to refer to the number on a medical examiner's license... examiner qualifies him or her to drive. This inconsistency has been clarified in today's final rule so that...
A Simulation of DNA Sequencing Utilizing 3M Post-It[R] Notes
ERIC Educational Resources Information Center
Christensen, Doug
2009-01-01
An inexpensive and equipment free approach to teaching the technical aspects of DNA sequencing. The activity described requires an instructor with a familiarity of DNA sequencing technology but provides a straight forward method of teaching the technical aspects of sequencing in the absence of expensive sequencing equipment. The final sequence…
ERIC Educational Resources Information Center
Appel, Victor H.; Roueche, John E.
The factors which contribute to the successful installation and assimilation of educational innovations were examined in relation to vocational/technical programs in eight post-secondary institutions. About 555 instructors, administrators, and non-teaching professional staff were surveyed regarding the level and extent of use of individualized…
Final Technical Report on the Institute for Advanced Study in Student Personnel Work.
ERIC Educational Resources Information Center
Callis, Robert
This document reports the planning, implementation, and evaluation of a 9-month institute held at the University of Missouri-Columbia to prepare participants (approximately 20) for leadership positions in student personnel work at junior colleges and technical institutes. The following aspects of the instructional program are discussed and…
Demonstration and Research Program for Teaching Young String Players. Final Report.
ERIC Educational Resources Information Center
Yarborough, William
This report explains a system for rapidly training beginning students in the technical aspects of playing a stringed instrument. The program also affords them a well-rounded, basic knowledge of music. A "numerical" method of notation and concentrated muscular exercises greatly speeded the technical learning process. The daily coordination of ear…
Troubleshooting Instruction in Vocational-Technical Education Via Dynamic Simulation. Final Report.
ERIC Educational Resources Information Center
Finch, Curtis R.
This study was designed to examine the feasibility of using simulation as a means of teaching vocational-technical students to detect and identify malfunctions in selected electrical and mechanical systems. A dynamic simulator was employed which features interchangeable panels and logic that permits the simulation of electrical or mechanical…
ERIC Educational Resources Information Center
Vivian, Neal E.
To upgrade research and research utilization competence of vocational educators, The Center for Vocational and Technical Education and The American Vocational Association planned four 1-week research training programs on: (1) Planning Vocational/Technical Education Programs Based on Manpower Research, (2) Patterns of Career Development as Applied…
Federal Register 2010, 2011, 2012, 2013, 2014
2013-01-18
... writing that it wishes to accept the designation. The final rule extends an FCU's response time from 30..., nonsubstantive technical amendments to NCUA's requirements for insurance regulation to reflect current agency... requirements for insurance regulation. These technical corrections are necessary to reflect current agency...
SURVEY OF INFORMATION ON VOCATIONAL AND TECHNICAL EDUCATION IN THE STATE OF ILLINOIS. FINAL REPORT.
ERIC Educational Resources Information Center
Corplan Associates, Chicago, IL. Technology Center.
THE BASIC OBJECTIVE OF THE SURVEY WAS TO GATHER INFORMATION HELPFUL IN PLANNING AND DEVELOPING VOCATIONAL AND TECHNICAL EDUCATION PRIMARILY WITHIN THE PUBLIC SCHOOL SYSTEM. OCCUPATIONAL NEEDS WERE IDENTIFIED FROM FORECASTS OF CHANGES IN CURRENT OCCUPATIONS, AN ANALYSIS OF THE IMPLICATIONS OF SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENTS, AND…
Federal Register 2010, 2011, 2012, 2013, 2014
2010-08-05
... Puget Sound, WA AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: This rule makes non-substantive... technical corrections to reflect the renaming of Sector Seattle to Sector Puget Sound as part of the Coast... organizational structure. Sector Seattle has been disestablished and Sector Puget Sound has been established in...
ERIC Educational Resources Information Center
Burger, Laura J.; And Others
The goal of this project was to develop, validate, and utilize a process for vertically articulating curriculum between secondary and post secondary levels of vocational technical education throughout the state of Minnesota. Procedures involved the identification of two areas of staff responsibility: research and development, and service to local…
Delivery of Instruction via Television. Final Report.
ERIC Educational Resources Information Center
Wisconsin State Board of Vocational, Technical, and Adult Education, Madison.
During 1985, the Wisconsin Board of Vocational, Technical, and Adult Education (VTAE) expanded its delivery of instruction via television. Approximately 3,000 persons at 170 sites throughout the United States and Canada participated in the VTAE's Technical School of the Air. In addition to delivering six to eight courses per semester, the network…
O*NET Final Technical Report. Volume I [and] Volume II [and] Volume III.
ERIC Educational Resources Information Center
Peterson, Norman G.; Mumford, Michael D.; Borman, Walter C.; Jeanneret, P. Richard; Fleishman, Edwin A.; Levin, Kerry Y.
This document contains the three volumes of the technical report for development of the prototype of the Occupational Information Network (O*NET), which is intended to replace the "Dictionary of Occupational Titles.""General Introduction" (Norman G. Peterson) presents an overview of O*NET's purpose, content, and structure.…
Federal Register 2010, 2011, 2012, 2013, 2014
2013-06-11
... NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES Institute of Museum and Library Services 45 CFR... Museum and Library Services AGENCY: Institute of Museum and Library Services (IMLS), NFAH. ACTION: Technical amendment; final rule. SUMMARY: The Institute of Museum and Library amends its grants regulations...
Final report on technical work accomplished under contract NASw-2953
NASA Technical Reports Server (NTRS)
Fredricks, R. W.
1977-01-01
A report is given on the technical work accomplished in the area of plasma physics. The subjects covered are: (1) oblique whistler instabilities, (2) current-limited electron beam injection, (3) three-dimensional ion sound turbulence, (4) theoretical aspects of sounder antenna operation and (5) whistler modes in bow shock structures.
Development of German-English Machine Translation System. Final Technical Report.
ERIC Educational Resources Information Center
Lehmann, Winfred P.; Stachowitz, Rolf A.
This report describes work on a pilot system for a fully automatic, high-quality translation of German scientific and technical text into English and gives the results of an experiment designed to show the system's capability to produce quality mechanical translation. The areas considered were: (1) grammar formalism, mainly involving the addition…
The Independent Technical Analysis Process Final Report 2006-2007.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Duberstein, Corey; Ham, Kenneth; Dauble, Dennis
2007-03-01
The Bonneville Power Administration (BPA) contracted with the Pacific Northwest National Laboratory (PNNL) to provide technical analytical support for system-wide fish passage information (BPA Project No. 2006-010-00). The goal of this project was to produce rigorous technical analysis products using independent analysts and anonymous peer reviewers. This project provided an independent technical source for non-routine fish passage analyses while allowing routine support functions to be performed by other well-qualified entities. The Independent Technical Analysis Process (ITAP) was created to provide non-routine analysis for fish and wildlife agencies and tribes in particular and the public in general on matters related tomore » juvenile and adult salmon and steelhead passage through the mainstem hydrosystem. The process was designed to maintain the independence of analysts and reviewers from parties requesting analyses, to avoid potential bias in technical products. The objectives identified for this project were to administer a rigorous, transparent process to deliver unbiased technical assistance necessary to coordinate recommendations for storage reservoir and river operations that avoid potential conflicts between anadromous and resident fish. Seven work elements, designated by numbered categories in the Pisces project tracking system, were created to define and accomplish project goals as follows: (1) 118 Coordination - Coordinate technical analysis and review process: (a) Retain expertise for analyst/reviewer roles. (b) Draft research directives. (c) Send directive to the analyst. (d) Coordinate two independent reviews of the draft report. (e) Ensure reviewer comments are addressed within the final report. (2) 162 Analyze/Interpret Data - Implement the independent aspects of the project. (3) 122 Provide Technical Review - Implement the review process for the analysts. (4) 132 Produce Annual Report - FY06 annual progress report with Pisces Disseminate (5) 161 Disseminate Raw/Summary Data and Results - Post technical products on the ITAP web site. (6) 185-Produce Pisces Status Report - Provide periodic status reports to BPA. (7) 119 Manage and Administer Projects - project/contract administration.« less
Site Operator technical report. Final report (1992--1996)
DOE Office of Scientific and Technical Information (OSTI.GOV)
NONE
1996-12-01
The Southern California Edison Company (SCE) and the US Department of Energy (DOE) entered into cooperative agreement No. DE-FC07-91ID13077 on August 23, 1991, which expired on August 3, 1996. This cooperative agreement provided SCE with DOE cofunding for participation in the DOE`s Electric and Hybrid Vehicle Site Operator Program. In return, SCE provided the DOE with quarterly progress reports which include operating and maintenance data for the electric (EVs) vehicles in SCE`s fleet. Herein is SCE`s final report for the 1992 to 1996 agreement period. As of September 1, 1996 the SCE fleet had 65 electric vehicles in service. Amore » total of 578,200 miles had been logged. During the agreement period, SCE sent the DOE a total of 19 technical reports (Appendix B). This report summarizes the technical achievements which took place during a long, productive and rewarding, relationship with the DOE.« less
7 CFR 1944.419 - Final grantee evaluation.
Code of Federal Regulations, 2010 CFR
2010-01-01
...) PROGRAM REGULATIONS (CONTINUED) HOUSING Self-Help Technical Assistance Grants § 1944.419 Final grantee... Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS..., application, this regulation, and any amendments. (a) This is a quantitative evaluation of the grantee to...
ECLSS medical support activities
NASA Technical Reports Server (NTRS)
Crump, William J.; Kilgore, Melvin V., Jr.
1991-01-01
During the period from April 10, 1990 to April 9, 1991, the Consortium for the Space Life Sciences provided technical assistance to the NASA/MSFC water recovery efforts. This assistance was in the form of literature reviews, technical recommendations, and presentations. This final report summarizes the activities completed during this period and identifies those areas requiring additional efforts. The tasks which the University of Alabama in Huntsville (UAH) water recovery team addressed were either identified by MSFC technical representatives or chosen from those outlined in the subject statement of work.
Switching gears and changing lanes
DOE Office of Scientific and Technical Information (OSTI.GOV)
Hammock, Earlene C.
2002-01-01
From musing over Shakespeare's fine lines and metaphors to teaching technical writing and editing, and finally, to cranking out scientific and technical documents, writing articles, and editing publications-how'd a nice girl like me end up in a place like this? Twice, after having prepared for an academic career of teaching and research, I found myself in a technical communications position-first, at the University of Texas at El Paso College of Engineering and later, at Los Alamos National Laboratory. What happened?
NASA Technical Reports Server (NTRS)
Flammia, Madelyn; Barclay, Rebecca O.; Pinelli, Thomas E.; Keene, Michael L.; Burger, Robert H.; Kennedy, John M.
1993-01-01
Until the recent dissolution of the Soviet Union, the Communist Party exerted a strict control of access to and dissemination of scientific and technical information (STI). This article presents models of the Soviet-style information society and the Western-style information society and discusses the effects of centralized governmental control of information on Russian technical communication practices. The effects of political control on technical communication are then used to interpret the results of a survey of Russian and U.S. aerospace engineers and scientists concerning the time devoted to technical communication, their collaborative writing practices and their attitudes toward collaboration, the kinds of technical documents they produce and use, their views regarding the appropriate content for an undergraduate technical communication course, and their use of computer technology. Finally, the implications of these findings for future collaboration between Russian and U.S. engineers and scientists are examined.
NASA Technical Reports Server (NTRS)
Flammia, Madelyn; Barclay, Rebecca O.; Pinelli, Thomas E.; Keene, Michael L.; Burger, Robert H.; Kennedy, John M.
1993-01-01
Until the recent dissolution of the Soviet Union, the Communist Party exerted a strict control of access to and dissemination of scientific and technical information. This article presents models of the Soviet-style information society and the Western-style information society and discusses the effects of centralized governmental control of information on Russian technical communication practices. The effects of political control on technical communication are then used to interpret the results of a survey of Russian and U.S. aerospace engineers and scientists concerning the time devoted to technical communication, their collaborative writing practices and their attitudes toward collaboration, the kinds of technical documents they produce and use, their views regarding the appropriate content for an undergraduate technical communication course, and their use of computer technology. Finally, the implications of these findings for future collaboration between Russian and U.S. engineers and scientists are examined.
Communication Platform Payload Definition (CPPD) study. Volume 2: Technical report
NASA Technical Reports Server (NTRS)
Hunter, E. M.; Driggers, T.; Jorasch, R.
1986-01-01
This is Volume 2 (Technical Report) of the Ford Aerospace & Communications Corporation Final Report for the Communication Platform Payload Definition (CPPD) Study program conducted for NASA Lewis Research Center under contract No. NAS3-24235. This report presents the results of the study effort leading to five potential platform payloads to service CONUS and WARC Region 2 traffic demand as projected to the year 2008. The report addresses establishing the data bases, developing service aggregation scenarios, selecting and developing 5 payload concepts, performing detailed definition of the 5 payloads, costing them, identifying critical technology, and finally comparing the payloads with each other and also with non-aggregated equivalent services.
Best Practices for Continuous Monitoring of Temperature and Flow in Wadeable Streams (Final Report)
This final report is a technical "best practices" document describing sensor deployment for and collection of continuous temperature and flow data at ungaged sites in wadeable streams. This document addresses questions related to equipment needs; configuration, placement, and ins...
This action corrects several technical errors and provides clarifying amendments to the final recycled used oil management standards rule. The final rule was published on September 10, 1992 (57 FR 41566).
75 FR 6839 - Technical Service Provider Assistance
Federal Register 2010, 2011, 2012, 2013, 2014
2010-02-12
... Executive Order 12866, the Office of Management and Budget determined that this final rule is not a... or are eligible to participate in conservation programs to help them make land management decisions...: Covered Programs. The interim final rule incorporated reference to the Agricultural Management Assistance...
Charlotte Circle Outreach. Final Report.
ERIC Educational Resources Information Center
Calhoun, Mary Lynne; Rose, Terry L.; Prendergast, Donna
This final report details the activities of the Charlotte Circle Outreach, a program designed to provide technical assistance and training to early intervention programs offering services to infants and young children with substantial disabilities, ages birth through two years. This mission was accomplished through cooperative planning with…
The Ecological Risk Assessment Support Center (ERASC) announced the release of the final report, Determination of the Biologically Relevant Sampling Depth for Terrestrial and Aquatic Ecological Risk Assessments. This technical paper provides defensible approximations fo...
ERIC Educational Resources Information Center
Phillips, Jane; Sewell, Charles
A broad technical assistance program has been established in 25 EDA (Economic Development Administration) contract counties and on the Choctaw Indian Reservation Nashoba County) to stimulate new job opportunities by solving operational problems which limit the expansion and diversification of existing industry; professional services in evaluating…
For Work-Force Training, a Plan to Give College Credit Where It's Due
ERIC Educational Resources Information Center
Sander, Libby
2008-01-01
After nearly three years of planning, Ohio's higher-education officials are finalizing an ambitious program to grant college credit for some technical courses offered at the state's adult-education centers. The program, called the Career-Technical Credit Transfer, is the latest in a string of state efforts to more closely link work-force training…
ERIC Educational Resources Information Center
Capital Systems Group, Inc., Rockville, MD.
The aim of this guide is to alert persons with an operational interest in scientific communication to new ideas, techniques, and equipment in the field of communication media and publications. The focus is on the dissemination of scientific information via the technical journal or its equivalent. Secondary dissemination of information such as…
ERIC Educational Resources Information Center
Technical Education Research Center, Waco, TX.
A project was conducted to develop a laboratory-based instructional system in physics for two-year technician programs that emphasizes both the analogies between basic physical principles and the applications of the principles in modern technology. The Unified Technical Concepts (UTC) system that was developed is (1) a reorganization of physics…
ERIC Educational Resources Information Center
BALDWIN, THOMAS S.
DURING THIS PERIOD FROM SEPTEMBER 1 THROUGH NOVEMBER 30, 1966, 35 FIELD CONSULTANTS COMPLETED ANALYSIS OF THEIR INDIVIDUAL TRADE AND TECHNICAL CURRICULUMS. THESE ANALYSES WERE DEVELOPED INTO AN OUTLINE TO SERVE AS A GUIDE FOR DEVELOPING ACHIEVEMENT TESTS. THE FINAL OUTLINE WAS DIVIDED INTO AS MANY DIFFERENT AREAS AS THE CONSULTANTS FELT NECESSARY…
Word Lists to Simplify Vocabulary of Technical Information. Final Report.
ERIC Educational Resources Information Center
Kincaid, J. Peter; And Others
This report describes eight word lists developed for use as part of the computer readability editing system (CRES), which was developed to serve as an author's aid in improving the ease of comprehending Navy technical manuals and training materials. The system has features which flag uncommon and misspelled words and long sentences, suggest simple…
77 FR 1889 - Drivers of CMVs: Restricting the Use of Cellular Phones; Technical Amendment
Federal Register 2010, 2011, 2012, 2013, 2014
2012-01-12
... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration 49 CFR Part 391 [Docket No. FMCSA-2010-0096] RIN 2126-AB29 Drivers of CMVs: Restricting the Use of Cellular Phones; Technical... Cellular Phones final rule (76 FR 75470) had a clerical error in Sec. 391.15(f)(1) that stated ``paragraph...
27 Years of Impact: Vocational-Technical Education in Ohio. Final Annual Report under Federal Law.
ERIC Educational Resources Information Center
Ohio State Council on Vocational Education, Westerville.
The Ohio Council on Vocational Education (OCOVE) was created to strengthen the career, vocational, and technical education services provided for Ohioans as a practical, efficient, and sure way to enhance the competitiveness of individual workers and the state and national economy. Some of the accomplishments of OCOVE during its 27 years of…
ERIC Educational Resources Information Center
McCormick, Robert W.
This study explored the relationship of vocational-technical educational institutions in Ohio with business and industry using high-technology applications. The study attempted to determine what high-technology applications will be adopted by Ohio's business and industry in the next 5 years, what experience the schools have had in working with…
ERIC Educational Resources Information Center
Karr, Alan
2011-01-01
NCES asked the National Institute of Statistical Sciences (NISS) to convene a technical panel of survey and policy experts to examine the NCES current and planned data dissemination strategies for confidential data with respect to: mandates and directives that NCES make data available; current and prospective technologies for protecting and…
ERIC Educational Resources Information Center
Institute for Local Self Government, Berkeley, CA.
To meet the manpower needs of local governments, the model developed for this project redirects national and technical education toward new careers programs. Designed by task forces of professional personnel, the model utilizes existing local government resources, including funds for new career activities. Accomplishments of the project include:…
Social Science Research Institutes in the Quality American University. Final Technical Report.
ERIC Educational Resources Information Center
Totman, Theodore L.
The technical report presents a chapter outline and thesis summary of an investigation of social science research institutes in American universities. The bulk of the report presents the thesis in four sections. Section I proposes a typology of organized social research units (OSRUs) in the 11 universities studied. Dimensions used to classify the…
ERIC Educational Resources Information Center
Austin Community Coll., TX.
The Expanding Horizons Project at Austin Community College successfully achieved its goals for Project Year 1994-95. During the year, the project accomplished the following: raised public awareness of the need to overcome gender bias, promoted career opportunities in nontraditional technical occupations to more than 1,200 prospective students,…
Technical Assistance and Training from the Document Design Project. Final Report.
ERIC Educational Resources Information Center
American Institutes for Research, Washington, DC.
Contained in this report is a description of the technical assistance and training phase of the Document Design Project, a program funded by the National Institute of Education and intended to address and correct the readability problems posed by public documents. The first section of the report provides background material on the assistance and…
ERIC Educational Resources Information Center
Farning, Max; Borden, Sally
A consortium of five Wisconsin Vocational, Technical, and Adult Education (VTAE) Districts (Gateway, Indianhead, Mid-State, Milwaukee, and Southwest) were utilized to identify, verify, and alleviate barriers to enrollment. A VTAE survey in 1976 identified six major reasons for individuals' failing to attend school after indicating an interest in…
Sociometric Clique Identification. Final Report.
ERIC Educational Resources Information Center
Kadushin, Charles
This report consists of four parts. The first part is a non-technical summary of the basic problem and an attempted solution. The second part is a technical review of the literature and a description of the basic algorithm used in the solution. The third part describes the use of the Sociogram System. The fourth part describes the use of CHAIN, a…
ERIC Educational Resources Information Center
Bakar, Ab Rahim; Mohamed, Shamsiah; Hamzah, Ramlah
2013-01-01
This study was performed to identify the employability skills of technical students from the Industrial Training Institutes (ITI) and Indigenous People's Trust Council (MARA) Skills Training Institutes (IKM) in Malaysia. The study sample consisted of 850 final year trainees of IKM and ITI. The sample was chosen by a random sampling procedure from…
ERIC Educational Resources Information Center
Baldus, Lorayne
A staff development program on gender equity was conducted for personnel in Wisconsin's technical colleges using the train-the-trainer method. The training took two approaches: a class for college personnel and career challenge training for project directors of single parent and displaced homemaker grants. The inservice class resulted in increased…
A Technical, User and Cost Comparison Study of Microfiche Duplicate Film Material. Final Report.
ERIC Educational Resources Information Center
Prevel, James J.
A technical, user and cost comparison study was undertaken to provide the Educational Resources Information Clearinghouse (ERIC) staff with data on silver halide, diazo, and vesicular type films for microfiche duplication. This information will allow ERIC to determine if diazo and/or vesicular films should be considered in producing ERIC duplicate…
DOE Office of Scientific and Technical Information (OSTI.GOV)
Fredrickson, Daniel C
2015-06-23
Final technical report for "Chemical Frustration: A Design Principle for the Discovery of New Complex Alloy and Intermetallic Phases" funded by the Office of Science through the Materials Chemistry Program of the Office of Basic Energy Sciences.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Pilger, R.H.
1984-06-25
This report contains the component Site-Specific Studies-Geophysics, Diagenesis, Geochemistry. Work for Site-Specific Studies is finished, and the results presented in this report are final. Individual studies have been entered separately into the data base. (ACR)
78 FR 54566 - Energy Labeling Rule
Federal Register 2010, 2011, 2012, 2013, 2014
2013-09-05
... FEDERAL TRADE COMMISSION 16 CFR Part 305 RIN 3084-AB03 Energy Labeling Rule AGENCY: Federal Trade Commission. ACTION: Final rule; correction. SUMMARY: The Federal Trade Commission published a final rule on July 23, 2013 revising its Energy Labeling Rule. This document makes a technical correction to the...
Wyoming Deaf/Blind Grant. Final Report.
ERIC Educational Resources Information Center
Whitson, Joanne B.
This final report describes activities and accomplishments of the Wyoming Deaf-Blind Grant, a 3-year federally supported project to identify children who have deaf-blindness and to provide technical assistance in the development of educational services for these children. Major accomplishments of the project included: identification of more…
DOE Office of Scientific and Technical Information (OSTI.GOV)
Not Available
1983-04-01
The document is one of six technical handbooks prepared by EPA to help government officials granting permits to build synfuels facilities, synfuels process developers, and other interested parties. They provide technical data on waste streams from synfuels facilities and technologies capable of controlling them. Process technologies covered in the manuals include coal gasification, coal liquefaction by direct and idirect processing, and the extraction of oil from shale. The manuals offer no regulatory guidance, allowing the industry flexibility in deciding how best to comply with environmental regulations.
NATO Scientific and Technical Information Service (NSTIS): functional description. Final report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Molholm, K.N.; Blados, W.N.; Bulca, C.
1987-08-01
This report provides a functional description of the requirements for a NATO Scientific and Technical Information Service (NSTIS). The user requirements and much of the background information in this report were derived primarily from interviews with more than 60 NATO Headquarters staff members between 2 March and 25 March 1987. In addition, representatives of the Supreme Headquarters Applied Powers Europe (SHAPE) Technical Centre (STC), the Supreme Allied Commander Atlantic (Anti-Submarine Warfare Research) Centre (SACLANTCEN), the NATO Communications and Information Systems Agency (NACISA), The Advisory Group for Aerospace Research and Development (AGARD), the U.S. Defense Technical Information Center (DTIC), and themore » Technical Documentation Center for the Armed Forces in the Netherlands (TDCK), were interviewed, either in person or by telephone.« less
Job Skills Education Program. Final Technical Report.
ERIC Educational Resources Information Center
Florida State Univ., Tallahassee. Center for Educational Technology.
This publication provides materials developed by a project designed to transfer a U.S. Army computer-based basic skills curriculum to applications in the vocational skills development of civilian adults. An executive summary of the final report describes the Job Skills Education Program (JSEP), which teaches academic skills that support vocational…
The Vocational Education Component of the Rhode Island Educational Management Information System.
ERIC Educational Resources Information Center
Galamaga, Donald P.; Bartolomeo, Paul A.
The document describes the implementation (Phase Two) of the Vocational Educational module--one component of an educational management information system. Phase Two entails the technical effort of final system design, final output specifications, edit specifications, system software selection, computer programing, systems documentation and the…
Services for Children with Deaf-Blindness in Louisiana. Final Performance Report.
ERIC Educational Resources Information Center
Teddlie, Charles
This final report describes activities and accomplishments of the Services for Children with Deaf-Blindness project, a 1-year federally supported project in Louisiana to improve identification and curriculum for these children by providing technical assistance and training to parents, school systems, and agency personnel. Project activities…
EPA's Ecological Risk Assessment Support Center (ERASC) announced the release of the final report, Evaluating Potential Exposures to Ecological Receptors Due to Transport of Hydrophobic Organic Contaminants in Subsurface Systems. This technical paper recommends several ty...
Federal Register 2010, 2011, 2012, 2013, 2014
2013-02-26
... has established under the Elementary and Secondary Education Act of 1965, as amended (20 U.S.C. 6301..., cooperative education, school-based enterprises, entrepreneurship, community service learning, and job... DEPARTMENT OF EDUCATION 34 CFR Chapter IV [Docket ID ED-2012-OVAE-0053] Final Requirements...
78 FR 73083 - Compassionate Release
Federal Register 2010, 2011, 2012, 2013, 2014
2013-12-05
... district in which the inmate was sentenced; and the final decision is subject to the general supervision... rule making a technical change to the regulations on February 28, 2013 (78 FR 13478). We now withdraw... the inmate was sentenced; and (2) clarifying that the final decision is subject to the general...
SIAM Conference on Geometric Design and Computing. Final Technical Report
DOE Office of Scientific and Technical Information (OSTI.GOV)
None
2002-03-11
The SIAM Conference on Geometric Design and Computing attracted 164 domestic and international researchers, from academia, industry, and government. It provided a stimulating forum in which to learn about the latest developments, to discuss exciting new research directions, and to forge stronger ties between theory and applications. Final Report
High/Scope Outreach Project. Final Report. October 1, 1983-September 30, 1984.
ERIC Educational Resources Information Center
High/Scope Educational Research Foundation, Ypsilanti, MI.
The final report reviews accomplishments of an outreach project designed to provide technical assistance and training to early childhood programs for handicapped children. The project features the Cognitively Oriented Preschool Curriculum, a developmental approach based on Piagetian theory and explained to build on the child's accomplishments. A…
ERIC Educational Resources Information Center
Van Beek, Dianne
This study examines courses in the marketing program at Northeast Wisconsin Technical College to compare the academic performance of students in traditional and learning community classroom settings. Two sections of students, a traditional 17-week course and a block scheduled 5-week course, served as the sample in the study. The block scheduling…
ERIC Educational Resources Information Center
Luger, Herbert P.; Booser, Ronald J.
A survey of the literature in the last ten years and interviews with library and security personnel indicated: (1)the problems of handling classified information in libraries have been scanted; (2) there is wide divergence in policies and practices of disseminating such materials; (3)interlibrary cooperation with respect to classified holdings is…
ERIC Educational Resources Information Center
Andreyka, Robert E.
This project's main objective was to field test competency-based vocational education (CBVE) student learning guides developed during 1979-1981 at Ridge Vocational-Technical Center (RVTC) (Florida). The learning guides were for six programs: clerical occupations, cosmetology, heavy duty truck/bus mechanics, industrial electricity, masonry, and…
Federal Register 2010, 2011, 2012, 2013, 2014
2013-03-25
... rule eliminates the nine-month ``dead zone'' for filing an inter partes review petition challenging a..., section 1(d) of the AIA Technical Corrections Act and this final rule eliminate the nine-month ``dead zone... the nine-month ``dead zone'' as to first-to-invent patents and reissue patents. Costs and Benefits...
ERIC Educational Resources Information Center
Bergen County Vocational-Technical High School, Hackensack, NJ.
This project was conducted to determine the vocational, technical, and scientific skills and knowledge needed to work with the fiber optics applications that are in all areas of technology. A research assistant was hired by the project director to collect data and develop a research base for the project. Information was gathered through a…
The California spotted owl: a technical assessment of its current status
Jared Verner; Kevin S. McKelvey; Barry R. Noon; R. J. Gutierrez; Gordon I. Jr. Gould; Thomas W. Beck
1992-01-01
This report is based an the Final Repart submitted on May 8, 1992 by the Technical Assessment Team to the interagency Steering Committee for the California Spotted Owl Assessment. The 13 chapters cover the assessment of the current status of the California spotted owl, its biology and habitat use, and forests where the subspecies occurs in the Sierra Nevada and...
ERIC Educational Resources Information Center
Technical Education Research Center, Waco, TX.
To evolve a new methodology and system for teaching physics to students aspiring to become (or to become more competent as) technicians in a variety of technologies, this research and development effort was initiated. The project's thesis stemmed from a notion that the study of physics would be more accepted and assimilated by students if concepts…
Establishment of the Center for Advanced Separation Technologies
DOE Office of Scientific and Technical Information (OSTI.GOV)
Christopher E. Hull
2006-09-30
This Final Technical Report covers the eight sub-projects awarded in the first year and the five projects awarded in the second year of Cooperative Agreement DE-FC26-01NT41091: Establishment of the Center for Advanced Separation Technologies. This work is summarized in the body of the main report: the individual sub-project Technical Progress Reports are attached as Appendices.
2017-11-01
MOKA WITH RISTRETTO ASSURED INFORMATION SECURITY, INC. NOVEMBER 2017 FINAL TECHNICAL REPORT APPROVED FOR PUBLIC RELEASE; DISTRIBUTION UNLIMITED...STINFO COPY AIR FORCE RESEARCH LABORATORY INFORMATION DIRECTORATE AFRL-RI-RS-TR-2017-223 UNITED STATES AIR FORCE ROME, NY 13441 AIR FORCE...report is available to the general public, including foreign nations. Copies may be obtained from the Defense Technical Information Center (DTIC
ERIC Educational Resources Information Center
Leisey, Sandra A.; Guinn, Nancy
At the request of the Air Force School of Aviation Medicine, a project was initiated to evaluate the current screening process used for entry into three medical technical training courses: Aeromedical Specialist, Environmental Health Specialist, and Physiological Training Specialist. A sample of 1,003 students were administered the General…
ERIC Educational Resources Information Center
Tuttle, Francis
Twenty-three instructors participated in an 8-week summer institute to develop their technical competency to teach the second year of a 2-year Technical Education Computer Science Program. Instructional material covered the following areas: (1) compiler languages and systems design, (2) cost studies, (3) business organization, (4) advanced…
DOE Office of Scientific and Technical Information (OSTI.GOV)
Vitkus, Timothy J.
2012-04-24
This guidance provides information on methodologies and the technical bases that licensees should consider for incorporating composite sampling strategies into final status survey (FSS) plans. In addition, this guidance also includes appropriate uses of composite sampling for generating the data for other decommissioning site investigations such as characterization or other preliminary site investigations.
ERIC Educational Resources Information Center
GRITTNER, FRANK; PAVLAT, RUSSELL
IN ORDER TO ASSIST NON-TECHNICAL PEOPLE IN SCHOOLS TO CONDUCT A FIELD CHECK OF LANGUAGE LABORATORY EQUIPMENT BEFORE THEY MAKE FINAL PAYMENTS, THIS MANUAL OFFERS CRITERIA, TESTS, AND METHODS OF SCORING THE QUALITY OF THE EQUIPMENT. CHECKLISTS ARE PROVIDED FOR EVALUATING CONSOLE FUNCTIONS, TAPE RECORDERS, AMPLIFIERS, SOUND QUALITY (INCLUDING…
Organic and Hybrid Organic Solid-State Photovoltaic Materials and Devices
2014-03-06
Microscopy Research, 2012, 7, 158-169. Organic photovoltaic materials, hybrid organic devices, solar cells 6 1 FINAL TECHNICAL REPORT 1... hybrids have potential applications in solar cells and may thus provide mobile energy sources for aircraft and soldier technologies. Modeling and...modeling and simulation developed in this project are encouraging further development. 2. Technical Activities Hybrid organic solar cells are an
Space station automation study: Autonomous systems and assembly, volume 2
NASA Technical Reports Server (NTRS)
Bradford, K. Z.
1984-01-01
This final report, prepared by Martin Marietta Denver Aerospace, provides the technical results of their input to the Space Station Automation Study, the purpose of which is to develop informed technical guidance in the use of autonomous systems to implement space station functions, many of which can be programmed in advance and are well suited for automated systems.
NASA Technical Reports Server (NTRS)
Sutter, James K.; Leidecker, Henning W.; Panda, Binayak; Piascik, Robert S.; Muirhead, Brian K.; Peeler, Debra
2009-01-01
The NESC eras requested by the NASA Jet Propulsion Laboratory (JPL) to conduct an independent review of the Mars Reconnaissance Orbiter (MRO) Thermal/Vacuum (T/V) Anomaly Assessment. Because the anomaly resulted in the surface contamination of the MRO, selected members of the Materials Super Problem Resolution Team (SPRT) and the NASA technical community having technical expertise relative to contamination issues were chosen for the independent review. The consultation consisted of a review of the MRO Project's reported response to the assessment findings, a detailed review of JPL technical assessment final report, and detailed discussions with the JPL assessment team relative to their findings.
Final Technical Report for DE-FG02-98ER45737
DOE Office of Scientific and Technical Information (OSTI.GOV)
Ade, Harald W.
This document represents the cumulative, final technical report for Grant No. DE-FG02- 98ER45737, the title of which has changed with each funding period, but the research pursued is within a coherent overall theme of methods and technique developments that exploit contrast at the carbon absorption edge to characterize complex organic materials and the use of these synchrotron radiation-based methods for important research challenges in polymer physics and Materials Science. The last three funding periods focused on organic devices and in particular organic solar cells (OSCs), owing to their extra-ordinarily complex morphology, yet high potential as a cheap and printable power-conversionmore » technology.« less
Environmental information acquisition and maintenance techniques: reference guide. Final report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Riggins, R.E.; Young, V.T.; Goran, W.D.
1980-08-01
This report provides a guide to techniques for collecting, using and maintaining data about each of the 13 environmental technical specialties in the Environmental Impact Computer System (EICS). The technical specialties are: (1) ecology, (2) environmental health, (3) air, (4) surface water, (5) ground water, (6) sociology, (7) economics, (8) earth science, (9) land use, (10) noise, (11) transportation, (12) aesthetics, and (13) energy and resource conservation. Acquisition techniques are classified by the following general categories: (1) secondary data, (2) remote sensing, (3) mathematical modeling, (4) field work, (5) mapping/maps and (6) expert opinion. A matrix identifies the most appropriatemore » techniques for collecting information on the EICS technical specialties. After selecting a method, the user may read an abstract of the report explaining that technique, and may also wish to obtain the original document for detailed information about applying the technique. Finally, this report offers guidelines on storing environmental information for future use, and on presenting that information effectively in environmental documents.« less
NEET-AMM Final Technical Report on Laser Direct Manufacturing (LDM) for Nuclear Power Components
DOE Office of Scientific and Technical Information (OSTI.GOV)
Anderson, Scott; Baca, Georgina; O'Connor, Michael
2015-12-31
Final technical report summarizes the program progress and technical accomplishments of the Laser Direct Manufacturing (LDM) for Nuclear Power Components project. A series of experiments varying build process parameters (scan speed and laser power) were conducted at the outset to establish the optimal build conditions for each of the alloys. Fabrication was completed in collaboration with Quad City Manufacturing Laboratory (QCML). The density of all sample specimens was measured and compared to literature values. Optimal build process conditions giving fabricated part densities close to literature values were chosen for making mechanical test coupons. Test coupons whose principal axis is onmore » the x-y plane (perpendicular to build direction) and on the z plane (parallel to build direction) were built and tested as part of the experimental build matrix to understand the impact of the anisotropic nature of the process.. Investigations are described 316L SS, Inconel 600, 718 and 800 and oxide dispersion strengthed 316L SS (Yttria) alloys.« less
NASA Astrophysics Data System (ADS)
Knobbs, C. G.; Grayson, D. J.
2012-06-01
There is mounting evidence to show that engineers need more than technical skills to succeed in industry. This paper describes a curriculum innovation in which so-called 'soft' skills, specifically inter-personal and intra-personal skills, were integrated into a final year mining engineering course. The instructional approach was designed to promote independent learning and to develop non-technical skills, essential for students on the threshold of becoming practising engineers. Three psychometric tests were administered at the beginning of the course to make students aware of their own and their classmates' characteristics. Substantial prescribed reading assignments preceded weekly group discussions. Several projects during the course required team work skills and application of content knowledge to real-world contexts. Results obtained from students' reflection papers, assignments related to 'soft' skills and end of course evaluations suggest that students' appreciation of the need for these skills, as well as their own perceived competence, increased during the course. Their ability to function as independent learners also increased.
Choi, Yoonha; Liu, Tiffany Ting; Pankratz, Daniel G; Colby, Thomas V; Barth, Neil M; Lynch, David A; Walsh, P Sean; Raghu, Ganesh; Kennedy, Giulia C; Huang, Jing
2018-05-09
We developed a classifier using RNA sequencing data that identifies the usual interstitial pneumonia (UIP) pattern for the diagnosis of idiopathic pulmonary fibrosis. We addressed significant challenges, including limited sample size, biological and technical sample heterogeneity, and reagent and assay batch effects. We identified inter- and intra-patient heterogeneity, particularly within the non-UIP group. The models classified UIP on transbronchial biopsy samples with a receiver-operating characteristic area under the curve of ~ 0.9 in cross-validation. Using in silico mixed samples in training, we prospectively defined a decision boundary to optimize specificity at ≥85%. The penalized logistic regression model showed greater reproducibility across technical replicates and was chosen as the final model. The final model showed sensitivity of 70% and specificity of 88% in the test set. We demonstrated that the suggested methodologies appropriately addressed challenges of the sample size, disease heterogeneity and technical batch effects and developed a highly accurate and robust classifier leveraging RNA sequencing for the classification of UIP.
Yang, Gai; Leicht, Anthony S; Lago, Carlos; Gómez, Miguel-Ángel
2018-01-01
The aim of this study was to identify the key physical and technical performance variables related to team quality in the Chinese Super League (CSL). Teams' performance variables were collected from 240 matches and analysed via analysis of variance between end-of-season-ranked groups and multinomial logistic regression. Significant physical performance differences between groups were identified for sprinting (top-ranked group vs. upper-middle-ranked group) and total distance covered without possession (upper and upper-middle-ranked groups and lower-ranked group). For technical performance, teams in the top-ranked group exhibited a significantly greater amount of possession in opponent's half, number of entry passes in the final 1/3 of the field and the Penalty Area, and 50-50 challenges than lower-ranked teams. Finally, time of possession increased the probability of a win compared with a draw. The current study identified key performance indicators that differentiated end-season team quality within the CSL.
NASA Technical Reports Server (NTRS)
Pinelli, Thomas E.; Glassman, Myron; Oliu, Walter E.; Barclay, Rebecca O.
1989-01-01
A study was undertaken that explored several aspects of technical communications in aeronautics. The study, which utilized survey research in the form of a self-administered questionnaire, was sent to 2,000 randomly selected members of the American Institute of Aeronautics and Astronautics. Six hundred and six usable questionnaires (30.3 percent) were received by the established cut off date. The study had five objectives. The first was to solicit the opinions of aeronautical engineers and scientists regarding the importance of technical communications to their profession; second, to determine their use and production of technical communications; third, to seek their views on the content of an undergraduate course in technical communications; fourth, to determine their use of libraries/technical information centers; and finally, to determine the use and importance of computer and information technology to them. The findings add considerable information to the knowledge of technical communications practices among aeronautical engineers and scientists and reinforce some of the conventional wisdom about technical communications and question other widely-held notions.
ERIC Educational Resources Information Center
Scheffler, F. L.; And Others
A feasibility study examined the capability of a computer-based system's handling of technical information pertinent to the design of instructional systems. Structured interviews were held to assess the information needs of both researchers and practitioners and an investigation was conducted of 10 computer-based information storage and retrieval…
ERIC Educational Resources Information Center
Weyers, Lori; Langerman, Philip
In 1989-90, the General Education Task Force of the Wisconsin Technical College System (WTCS) was convened to determine the role of the general education curriculum in the attainment of skills that enhance the likelihood of success among technical college graduates in their careers, homes and communities. The Task Force consisted of at least one…
ERIC Educational Resources Information Center
Keyes, Jose Luis; Collins, Carla
The Bilingual Academic and Technical Education for Youth (BATEY) Program completed its third and final year of funding at Adlai E. Stevenson High School, Bronx, New York. In 1982-83, the program offered bilingual instruction and support services to approximately 300 Hispanic students of limited English proficiency in grades 9-12. Although the…
Federal Register 2010, 2011, 2012, 2013, 2014
2012-09-17
...-0152] Technical Report on Fatality Risk, Mass, and Footprint of Model Year 2000-2007 Passenger Cars and..., and Footprint in Model Year 2000-2007 Passenger Cars and LTVs--Final Report. DATES: Comments must be... comprised MY 2000-2007 cars and LTVs in CY 2002-2008 crashes. Fatality rates were derived from FARS data, 13...
ERIC Educational Resources Information Center
Innovative Systems Research, Inc., Pennsauken, NJ.
Twelve different conversion plans were delineated and evaluated in the performance of this study effort. An indepth technical, financial, and cost/benefit analysis was included. One plan was determined to be distinctively more cost-effective than any of the other plans because it provided a technically feasible system that assured the maximum…
Technical Feasibility Study of an Effective Low-Toxicity Obscurant Material
2012-08-01
2. REPORT TYPE FINAL 3. DATES COVERED (From - To) 04/2011 – 07/2012 4 . TITLE AND SUBTITLE 5a. CONTRACT NUMBER W912HQ-11-C-0034 Technical...6 4 Work Package 3 – Laboratory-scale tests...chemistry to sea-salt aerosol. 4 The major component of sea-salt is sodium chloride. Sodium chloride can be very simply generated by the combustion
ERIC Educational Resources Information Center
Ira, Nejat
2015-01-01
The purpose of the research is to identify the attitudes of the students at Kocaeli University towards closing down of Technical Education Faculty. These faculties were decided to be closed down in 2009. Since then, both the students and the instructors have been in ambiguity about their future careers. This situation has also affected them…
ERIC Educational Resources Information Center
Foley, John P., Jr.
The document presents immediate and long range plans for the systematic injection of Symbolic Integrated Maintenance Systems (SIMS) and Fully Proceduralized Job Performance Aids (FPJPA) materials into the current technical order system and the inclusion of job-oriented training matched to FPJPA into the training system of the Air Force. The text…
2011-02-01
NORTH ATLANTIC TREATY ORGANISATION RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-129)TP/349 www.rto.nato.int RTO TECHNICAL REPORT TR...Final Report of Task Group HFM-129. Published February 2011 Distribution and Availability on Back Cover...NORTH ATLANTIC TREATY ORGANISATION RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-129)TP/349 www.rto.nato.int RTO TECHNICAL REPORT TR-HFM
ERIC Educational Resources Information Center
Pucel, David J.; And Others
Using post-secondary vocational education students as the populations, these two sub-studies of the Project MINI-SCORE sought to determine the extent to which pre-enrollment standardized test data can be used to predict vocational success. For the purpose of the study, vocational success was defined either as successful graduation or successful…
Learning from Experience Case Studies of the Hyper-X Project
NASA Technical Reports Server (NTRS)
Peebles, Curtis
2009-01-01
The Hyper-X project (X-43A) provides a number of "lessons learned" which can be applied to other aerospace project. The specific areas examined were the selection of the goals of the Hyper-X. How the technical unknowns and assumptions were handled. The final lesson was the ambiguous nature of risk assessment, and how trying to remove a technical unknown can have unintended consequences.
Helicopter Dynamic Performance Program. Volume 2. User’s Manual
1980-07-01
Technologies Corporation Stratford, Connecticut 06602 July 1980 Final Report / • ., . Approved for public release; I distribution unlimited. ; A - Prepared...for : APPLIED TECHNOLOGY LABORATORY U. S. ARMY RESEARCH AND TECHNOLOGY LABORATORIES (AVRADCOM) Fort Eustis, Va. 23604 80 8 27 D APPLIED TECHNOLOGY ... Technology Division. Technic31 review of this report was also pro- vided by Messrs. W_ A. Pleasants of Design Integration and Analysis Technical Area
A Study of Placing Army Requirements on Contract
2016-04-01
perhaps being unable to control the external environment, has management authority over business and technical aspects of a specific program. The PM...research is the last element of the planning phase addressing both business and technical considerations. The acquisition team equips themselves with...report is the final product utilizing documented findings and supports the business strategy (DoD, Guidebook for the Acquisition of Services, 2011
ERIC Educational Resources Information Center
Overlock, Terrence H., Sr.
To determine the effect of collaborative learning methods on the success rate of physics students at Northern Maine Technical College (NMTC), a study was undertaken to compare the mean final exam scores of a students in a physics course taught by traditional lecture/lab methods to those in a group taught by collaborative techniques. The…
ERIC Educational Resources Information Center
Ibrahim, Wan Nur Azlina; Bakar, Ab. Rahim; Asimiran, Soaib; Mohamed, Shamsiah; Zakaria, Noor Syamilah
2015-01-01
The purpose of this study was to determine the entrepreneurial intention level of vocational and technical students in Malaysia. A total of 289 final year students who were enrolled at two different TVET institutions (community colleges and National Youth and Skills Institutes) were chosen to participate in the study. The findings indicated that…
ERIC Educational Resources Information Center
Maney, Thomas J.; Boyd, Linda
Personal interviews with each employer of health care workers in the Nicolet Vocational, Technical and Adult Education (VTAE) District were conducted to determine: (1) the extent of need for trained health occupations personnel in the district; (2) the projected need for such personnel in the future; (3) which specific health occupations currently…
ERIC Educational Resources Information Center
Touche Ross and Co., Washington, DC.
Technical assistance provided to loan guarantee agencies by Touche Ross and Co. under contract to the U.S. Office of Education (OE) is described. Objectives of the report are: to summarize the work performed and the results of each of the projects that were conducted; to describe certain prototype systems that were developed for the guarantee…
ERIC Educational Resources Information Center
HECKER, DONALD L.; TAYLOR, RONALD G.
THE PURPOSE OF THE PROJECT WAS TO STUDY THE DIFFERENCES AND SIMILARITIES IN INTEREST, ABILITY, AND PREVIOUS ACHIEVEMENT PATTERNS OF 941 MEN STUDENTS WHO ENROLLED IN VARIOUS TECHNICAL AND ASSOCIATE DEGREE 2-YEAR PROGRAMS AND TO DETERMINE WHICH VARIABLES COULD DIFFERENTIATE BETWEEN STUDENTS WHO COMPLETED, CHANGED TO AN ALTERNATE PROGRAM, OR WITHDREW…
Sub-Project 7 of Wisconsin 553, Part F, EPDA--Industry/Exchange Program: Final Report.
ERIC Educational Resources Information Center
Wisconsin Univ. - Stout, Menomonie. Center for Vocational, Technical and Adult Education.
The final report describes a study to develop a statewide teacher/industry exchange program in Wisconsin that would provide vocational-technical instructors with occupational experience opportunities. Three plans for implementing the program were developed: (A) swapping the positions of a business or industrial person with that of a vocational…
2+2+2 Dissemination Project Final Report.
ERIC Educational Resources Information Center
Sloane, Diana; And Others
An overview is provided in this three-part final report of a project designed to disseminate information to assist California high schools, Regional Occupation Programs, and colleges in developing and strengthening 2+2+2 programs. Part I reviews the following project objectives: (1) hire a project coordinator and technical assistant; (2) develop a…
State Compensatory Education. Final Technical Report 1975-76. Appendixes.
ERIC Educational Resources Information Center
Austin Independent School District, TX. Office of Research and Evaluation.
This material consists of ten appendixes to the Final Report for 1976-77 on the implementation of the Texas state-supported compensatory education program in the Austin Independent School District as evaluated by that district's Office of Research and Evaluation. The instruments used in the evaluation process are described or presented in full,…
ERIC Educational Resources Information Center
Kelly, Dotty; Guerrero, Vincent Leon
This final report describes activities and accomplishments of the Pacific Basin Deaf-Blind Project, a 3-year federally funded project to provide technical assistance to public and private agencies, institutions, and organizations providing early intervention, educational, transitional, vocational, early identification, and related services to…
ERIC Educational Resources Information Center
Stremel, Kathleen; Wilson, Rebecca
This final report describes a federally funded 3-year project for integrating related services within educational objectives for children (ages 3-10) with dual vision and hearing impairments. A Training-Utilization model of inservice training and technical assistance was developed, implemented, and evaluated to address the communication needs of…
ADAAG Review Federal Advisory Committee Recommendations for a New ADAAG. Final Report.
ERIC Educational Resources Information Center
Architectural and Transportation Barriers Compliance Board, Washington, DC.
This report presents the final recommendations, in the form of a new set of guidelines, of a federal advisory committee concerning revisions to the Americans with Disabilities Act Accessibility Guidelines (ADAAG). The guidelines provide scoping and technical requirements for the design, construction, and alteration of sites, facilities, buildings,…
76 FR 78141 - Pilot, Flight Instructor, and Pilot School Certification; Technical Amendment
Federal Register 2010, 2011, 2012, 2013, 2014
2011-12-16
... command under IFR or in weather conditions less than the minimums prescribed for VFR. Finally, this... pilot in command under IFR or in weather conditions less than the minimums prescribed for VFR. Under... command under IFR or in weather conditions less than the minimums prescribed for VFR. In the 2009 final...
76 FR 10516 - Transfer and Reorganization of Bank Secrecy Act Regulations-Technical Amendment.
Federal Register 2010, 2011, 2012, 2013, 2014
2011-02-25
... concerning mutual funds and the other concerning the confidentiality of a report of suspicious activity (SAR... compliance date of the mutual fund rule. Additionally, the Chapter X Final Rule contained an inadvertent..., 2010, FinCEN issued a final rule to include mutual funds within the general definition of ``financial...
Providing Computer-Based Information Services to an Academic Community. Final Technical Report.
ERIC Educational Resources Information Center
Bayer, Bernard
The Mechanized Information Center (MIC) at the Ohio State University conducts retrospective and current awareness searches for faculty, students, and staff using data bases for agriculture, chemistry, education, psychology, and social sciences, as well as a multidisciplinary data base. The final report includes (1) a description of the background…
Project CREATE Final Report. Cooperative Resources To Enhance Access to Technology Education.
ERIC Educational Resources Information Center
Hampden County Employment and Training Consortium, Springfield, MA.
These materials have been developed by Project CREATE (Cooperative Resources to Enhance Access to Jobs through Technical Education), a demonstration program designed to develop a network, specific activities, and resources that would provide education and support services to a wide audience. A 13-page final report describes the hands-on training…
Digital-Computer Processing of Graphical Data. Final Report.
ERIC Educational Resources Information Center
Freeman, Herbert
The final report of a two-year study concerned with the digital-computer processing of graphical data. Five separate investigations carried out under this study are described briefly, and a detailed bibliography, complete with abstracts, is included in which are listed the technical papers and reports published during the period of this program.…
48 CFR 1852.235-73 - Final Scientific and Technical Reports.
Code of Federal Regulations, 2012 CFR
2012-10-01
... regarding appropriate electronic formats for final reports is available at http://www.sti.nasa.gov under....nasa.gov under the “Get Help” link. (e) In accordance with paragraph (d) of the Rights in Data—General... contained in NPR 2200.2, Guidelines for Documentation, Approval, and Dissemination of NASA Scientific and...
48 CFR 1852.235-73 - Final Scientific and Technical Reports.
Code of Federal Regulations, 2013 CFR
2013-10-01
... regarding appropriate electronic formats for final reports is available at http://www.sti.nasa.gov under....nasa.gov under the “Get Help” link. (e) In accordance with paragraph (d) of the Rights in Data—General... contained in NPR 2200.2, Guidelines for Documentation, Approval, and Dissemination of NASA Scientific and...
48 CFR 1852.235-73 - Final Scientific and Technical Reports.
Code of Federal Regulations, 2011 CFR
2011-10-01
... regarding appropriate electronic formats for final reports is available at http://www.sti.nasa.gov under....nasa.gov under the “Get Help” link. (e) In accordance with paragraph (d) of the Rights in Data—General... contained in NPR 2200.2, Guidelines for Documentation, Approval, and Dissemination of NASA Scientific and...
48 CFR 1852.235-73 - Final Scientific and Technical Reports.
Code of Federal Regulations, 2014 CFR
2014-10-01
... regarding appropriate electronic formats for final reports is available at http://www.sti.nasa.gov under....nasa.gov under the “Get Help” link. (e) In accordance with paragraph (d) of the Rights in Data—General... contained in NPR 2200.2, Guidelines for Documentation, Approval, and Dissemination of NASA Scientific and...
10 CFR 52.79 - Contents of applications; technical information in final safety analysis report.
Code of Federal Regulations, 2010 CFR
2010-01-01
... assurance program will be implemented; (26) The applicant's organizational structure, allocations or... presents a safety analysis of the structures, systems, and components of the facility as a whole. The final... contain an analysis and evaluation of the major structures, systems, and components of the facility that...
PFBC HGCU Test Facility. Technical progress report No. 24, Third quarter, CY 1995
DOE Office of Scientific and Technical Information (OSTI.GOV)
NONE
This is the twenty-fourth and final Technical Progress Report submitted to the Department of Energy (DOE) in connection with the cooperative agreement between the DOE and Ohio Power Company for the Tidd PFBC Hot Gas Clean Up Test Facility. This report covers the work completed during the Third Quarter of CY 1995. All activity this quarter was directed toward the completion of the program final report. A draft copy of the final report was forwarded to DOE during this quarter, and DOE submitted their comments on the report to AEPSC. DOE requested that Westinghouse write an appendix to the reportmore » covering the performance of the fail-safe regenerator devices during Tad operation, and Westinghouse subsequently prepared the appendix. Additional DOE comments were incorporated into the report, and it will be issued in camera-ready form by the end of October, 1995, which is the program end date. Appendix 1 presents the results of filter candle posttest examination by Westinghouse performed on selected filter candles following final shutdown of the system.« less
Lessons for surgeons in the final moments of Air France Flight 447.
Bhangu, Aneel; Bhangu, Sonia; Stevenson, James; Bowley, Douglas M
2013-06-01
All surgeons make mistakes, and learning from critical incidents may help improve performance. The present study aimed to highlight lessons for surgeons from analysis of the final moments of Air France Flight 447. All of the authors work in teams and situations where safety, technical performance, and non-technical skills are critical. This review was born out of discussions regarding the events of Flight 447; specifically, whether the airline disaster was relevant to their work, and whether they could learn anything from it. The study is based on review of the crash reports of Flight 447, which lost flight speed indication after formation of ice prevented air from entering flight speed indicators during a storm. Following a subsequent stall, the aircraft fell at a rate of >10,000 feet/min until it crashed into the Atlantic Ocean, killing 228 passengers and crew. There were errors in decision making, reasoning, communication, and teamwork. Such non-technical skills failures have been recognized previously and can be addressed by existing non-technical skills training. A reliance on autopilot meant that the pilots were unfamiliar with high-altitude flying when the autopilot is disengaged. They were unprepared for and affected by such a sudden and serious problem; an event called "surprise and startle" by the accident investigation. The absence of the senior pilot (who was on a scheduled break) in the critical final minutes slowed error recognition and recovery. Unintended consequences of modern safety strategies may be under-recognized and can lead to adverse events. Both simulation-based and non-simulation-based training should include "surprise and startle" events beyond the scenarios trainees might expect. Likewise, in the face of increasing reliance on modern technology, surgeons should ensure that they would be able to perform procedures in the absence of such technologies. Specific training may improve surgeons' non-technical skills, and recognition of such skills could also be used to help select future surgeons.
Enhancement of the Probabilistic CEramic Matrix Composite ANalyzer (PCEMCAN) Computer Code
NASA Technical Reports Server (NTRS)
Shah, Ashwin
2000-01-01
This report represents a final technical report for Order No. C-78019-J entitled "Enhancement of the Probabilistic Ceramic Matrix Composite Analyzer (PCEMCAN) Computer Code." The scope of the enhancement relates to including the probabilistic evaluation of the D-Matrix terms in MAT2 and MAT9 material properties card (available in CEMCAN code) for the MSC/NASTRAN. Technical activities performed during the time period of June 1, 1999 through September 3, 1999 have been summarized, and the final version of the enhanced PCEMCAN code and revisions to the User's Manual is delivered along with. Discussions related to the performed activities were made to the NASA Project Manager during the performance period. The enhanced capabilities have been demonstrated using sample problems.
The anatomy of the gyroscope, part 3
NASA Astrophysics Data System (ADS)
Cousins, Frank W.; Hollington, John L.
1990-03-01
The gyroscope and its applications are collated with the technical information to be found in the patent literature, augmented by that found in textbooks and technical journels. A detailed consideration is given to early mechanical gyroscopes. Included is a discussion of gyroscopic gears, vibratory gyroscopes, interferometric gyroscopes, and the use of the gyroscope in optical and related devices and deals finally with the difficult problem of gyroscopic inertial drives.
Query Storage and Relay in Research Root (LACREND RR)
2017-05-30
QUERY STORAGE AND RELAY IN RESEARCH ROOT (LACREND-RR) UNIVERSITY OF SOUTHERN CALIFORNIA MAY 2017 FINAL TECHNICAL REPORT APPROVED FOR PUBLIC ...exempt from public affairs security and policy review in accordance with SAF/AQR memorandum dated 10 Dec 08 and AFRL/CA policy clarification...memorandum dated 16 Jan 09. This report is available to the general public , including foreign nationals. Copies may be obtained from the Defense Technical
ERIC Educational Resources Information Center
Lyons, Thomas M.; Knight, Glen A.
A model project was conducted to demonstrate how Chrysler, in partnership with the education community and the government, could provide technical training to enable displaced workers to contribute to the "H-Body" car launch, to improve their job skills, and to enhance their future employability. The training was conducted on a pilot…
Heat pump assisted geothermal heating system for Felix Spa, Romania
DOE Office of Scientific and Technical Information (OSTI.GOV)
Rosca, Marcel; Maghiar, Teodor
1996-01-24
The paper presents a pre-feasibility type study of a proposed heat pump assisted geothermal heating system for an average hotel in Felix Spa, Romania. After a brief presentation of the geothermal reservoir, the paper gives the methodology and the results of the technical and economical calculations. The technical and economical viability of the proposed system is discussed in detail in the final part of the paper.
Heat pump assisted geothermal heating system for Felix Spa, Romania
DOE Office of Scientific and Technical Information (OSTI.GOV)
Rosca, M.; Maghiar, T.
1996-12-31
The paper presents a pre-feasibility type study of a proposed heat pump assisted geothermal heating system for an average hotel in Felix Spa, Romania. After a brief presentation of the geothermal reservoir, the paper gives the methodology and the results of the technical and economical calculations. The technical and economical viability of the proposed system is discussed in detail in the final part of the paper.
The 1990 Johnson Space Center bibliography of scientific and technical papers
NASA Technical Reports Server (NTRS)
1991-01-01
Abstracts are presented of scientific and technical papers written and/or presented by L. B. Johnson Space Center (JSC) authors, including civil servants, contractors, and grantees, during the calendar year of 1990. Citations include conference and symposium presentations, papers published in proceedings or other collective works, seminars, and workshop results, NASA formal report series (including contractually required final reports), and articles published in professional journals.
ERIC Educational Resources Information Center
General Electric Co., Philadelphia, PA. Space Div.
The purpose of this study was to establish the technical and economic feasibility of using solar energy for the heating and cooling of buildings and to provide baseline information for the widespread application of solar energy. The initial step in this program was a study of the technical, economic, societal, legal, and environmental factors…
DOT National Transportation Integrated Search
2015-04-01
This document is the final project technical report for the development and field testing of the Response, Emergency Staging, Communications, Uniform Management, and Evacuation (R.E.S.C.U.M.E.) application bundle, with a focus on the Incident Zone (I...
Review of Military Mountain Medicine Technology and Research Barriers
2011-09-01
NORTH ATLANTIC TREATY ORGANISATION RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-146)TP/387 www.rto.nato.int RTO TECHNICAL REPORT TR...montagne et les freins à la recherche) Final Report of Task Group HFM-146. Published September 2011 Distribution and Availability...323(HFM-146)TP/387 www.rto.nato.int RTO TECHNICAL REPORT TR-HFM-146 Review of Military Mountain Medicine Technology and Research Barriers (Point
ERIC Educational Resources Information Center
BIVONA, WILLIAM A.
THIS REPORT PRESENTS AN ANALYSIS OF OVER EIGHTEEN SMALL, INTERMEDIATE, AND LARGE SCALE SYSTEMS FOR THE SELECTIVE DISSEMINATION OF INFORMATION (SDI). SYSTEMS ARE COMPARED AND ANALYZED WITH RESPECT TO DESIGN CRITERIA AND THE FOLLOWING NINE SYSTEM PARAMETERS--(1) INFORMATION INPUT, (2) METHODS OF INDEXING AND ABSTRACTING, (3) USER INTEREST PROFILE…
Air Force Research Laboratory Wright Site Guide to Technical Publishing
2005-04-01
Scientific and Technical Reports—Elements, Organization, and Design manual (and a version modified for documents generated for AFRL) • Merriam-Webster’s...notice page --SF 298 --original graphics /halftones Indicate the following on the letter of transmittal sheet: --quantity of copies required for...Elements, Organization and Design ? The WRS CDRL for a final report requires that the standard be followed. The only exception is SBIR Phase 1
The Effect of Drycleaning Moisture on Fused Cloth Systems
1989-03-01
TECHNICAL REPORT NATICK/TR-89/024 et, THE EFFECT OF DRYCLEANING MOISTURE ON FUSED CLOTH SYSTEMS BY ELIZABETH J. MORELAND International...MOISTUP.E ON FUSED CLOTH SYSTEMS 12. PERSONAL AUTMOR(S) Elizabeth J. MorelanJ 13«. TYPE OF REPORT Final Technical Report 13b. TIME COVERED...This project was initiated to investigate the effect of moisture in drycleaning systems on preselected fused cloth structures. Adverse surface
ERIC Educational Resources Information Center
Kazarian, Edward N., Ed.; Ward, Darrell, L., Ed.
The proceedings of this seminar, attended by state directors and other leaders in vocational and technical education from 41 states, the District of Columbia, Saipan and Puerto Rico, contain presentations by 18 leaders from industry, government, and education. Specific objectives were to: (1) provide a forum for presentations concerning personnel…
Leader Identity, Individual Differences, and Leader Self-Development
2012-09-01
and Social Sciences Approved for public release; distribution is unlimited. U.S. Army Research Institute for the Behavioral and Social ... Social Sciences, Attn: DAPC-ARI-MS, 6000 6th Street, Fort Belvoir, VA 22060-5586. FINAL DISPOSITION: Destroy this Technical Report when it is no...longer needed. Do not return it to the U.S. Army Research Institute for the Behavioral and Social Sciences. NOTE: The findings in this Technical
Optimizing Operational Physical Fitness (Optimisation de L’Aptitude Physique Operationnelle)
2009-01-01
NORTH ATLANTIC TREATY ORGANISATION RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-080)TP/200 www.rto.nato.int RTO TECHNICAL REPORT TR... RESEARCH AND TECHNOLOGY ORGANISATION AC/323(HFM-080)TP/200 www.rto.nato.int RTO TECHNICAL REPORT TR-HFM-080 Optimizing Operational Physical...Fitness (Optimisation de l’aptitude physique opérationnelle) Final Report of Task Group 019. ii RTO-TR-HFM-080 The Research and
Rating Training Continuum: Development Procedures
1992-01-01
During the workshop, the SMEs will need to review technical documents to refresh their memory and resolve any technical issues. It is important that a...endorsement. 7 additional memory space was very useful. The computers with the 80-megabyte hard disk drive were used to collect all final PPPs and TLAs...description, general, and documentation). If you reed to refresh your memory , refer to Figure 3-5 for the definitions associated with the indirectly
Test Plan Development for Plastic Ammunition Containers. Volume 1
1989-03-15
1850 Black Canyon Stage¶I Packaging division (SMCAR-AEP) Picatinny Phoenix, Arizona 85027 Arsenal, New Jersey 07806-5000 86. NAME OF FUNDING...packaging containers. The report is presented in two separate volumes. Volume I contains the Final Technical Report and includes the analysis of... Division of the U.S. Army Armament Research, Development and Engineering Center. Mr. Jasper C. Griggs and Mr. D. E. Jones served as technical consultants
DOE Office of Scientific and Technical Information (OSTI.GOV)
Richman, M.
1996-08-01
Granular flows of nearly elastic, identical spheres down bumpy inclines are described. A numerical technique is developed to overcome the difficulties associated with the ill-defined `tops` of these flows.
TEAM TRAINING. FINAL TECHNICAL REPORT FEBRUARY 1966-FEBRUARY 1967.
ERIC Educational Resources Information Center
BRIGGS, GEORGE E.; JOHNSTON, WILLIAM A.
THIS IS THE FINAL REPORT ON A FOUR-YEAR PROGRAM OF LABORATORY RESEARCH ON TEAM TRAINING IN A COMBAT INFORMATION CENTER (CIC) CONTEXT. THE RESEARCH LITERATURE ON TEAM TRAINING IS REVIEWED, AND A SET OF CONCLUSIONS IS DRAWN WITH REGARD TO TEAM PERFORMANCE AS A FUNCTION OF TASK, TRAINING, AND COMMUNICATIONS VARIABLES. IN ADDITION, THE IMPLICATIONS…
DOE Office of Scientific and Technical Information (OSTI.GOV)
DiNunzio, Camillo A.; Gupta, Abhinav; Golay, Michael
2002-11-30
This report presents a summation of the third and final year of a three-year investigation into methods and technologies for substantially reducing the capital costs and total schedule for future nuclear plants. In addition, this is the final technical report for the three-year period of studies.
Validation of Technical Recommendations. Final Report. ISSOE Managing Student Progress.
ERIC Educational Resources Information Center
Ridley, Dennis; And Others
This report is organized to provide a complete, logical presentation of the major steps taken in the third phase of research on dynamics of dissemination of the Instructional Support System for Occupational Education (ISSOE). It reports on the confirmation and validation of the Phase II Final Report on Dissemination Issues and the Phase III…
ERIC Educational Resources Information Center
Georgia State Dept. of Education, Atlanta.
This final report describes activities and accomplishments of the Georgia Deaf-Blind Project, a 3-year federally supported project encompassing 159 counties and providing technical assistance to 237 infants, children, and youth with deaf-blindness along with their families and their service providers. Project accomplishments included: (1) more…
Federal Register 2010, 2011, 2012, 2013, 2014
2013-12-03
..., 2011 Tornado in Joplin, Missouri AGENCY: National Institute of Standards and Technology, United States... for public comment of the draft final report of the investigation conducted by NIST into the tornado... found on the Joplin Investigation Web page http://www.nist.gov/el/disasterstudies/weather/joplin_tornado...
ERIC Educational Resources Information Center
Indiana State Univ., Terre Haute. Blumberg Center for Interdisciplinary Studies in Special Education.
This final report describes activities and accomplishments of the Indiana Deaf-Blind Services Project, a 4-year federally funded project to enhance and further develop coordinated direct services to children and youth, birth through 2 and ages 18 through 21. The project also was designed to provide technical assistance to public and private…
ERIC Educational Resources Information Center
Walsh, Kath; Rebaczonok-Padulo, Michael
1993-01-01
Ngee Ann Polytechnic, a leading postsecondary technical institution in Singapore, offers English for academic and occupational purposes to prepare students for writing their final year projects. This article discusses the approaches used in Mechanical Engineering and Biotechnology projects. A sample exercise is appended. (Contains two references.)…
Computation in the Wild: Moving Beyond the Metaphor
2006-03-01
AFRL-IF-RS-TR-2006-105 Final Technical Report March 2006 COMPUTATION IN THE WILD: MOVING BEYOND THE METAPHOR University...Government. AIR FORCE RESEARCH LABORATORY INFORMATION DIRECTORATE ROME RESEARCH SITE ROME, NEW YORK STINFO FINAL REPORT This... report has been reviewed by the Air Force Research Laboratory, Information Directorate, Public Affairs Office (IFOIPA) and is releasable to the National
Illinois State Project: Services for Children with Deaf-Blindness Program. Final Performance Report.
ERIC Educational Resources Information Center
Dorsey, Christine
This final report describes activities and accomplishments of the Illinois state project on services for children with deaf-blindness, a 3-year federally supported project to address the needs of children (ages birth to 21) who are deaf-blind. Project activities included inservice training and technical assistance, development of a comprehensive…
PROJECT PATS: "Potentially Academically Talented Students." Final Technical Report, 10/1/80-6/30/82.
ERIC Educational Resources Information Center
Pfeffer, J. S.; And Others
The final report describes a 2-year pilot project to identify and program for disadvantaged potentially gifted students in grades 5-8. Two goals of the program are stated: (1) to establish a working partnership among public and nonpublic schools and higher education; and (2) to integrate disadvantaged potentially gifted with nondisadvantaged…
Exploration studies technical report, FY1988 status. Volume 1: Technical summary
NASA Technical Reports Server (NTRS)
1988-01-01
The Office of Exploration (OEXP) at NASA Headquarters has been tasked with defining and recommending alternatives for an early 1990's nationaL decision on a focused program of human exploration of the solar system. The Mission Analysis and System Engineering (MASE) group, which is managed by the Exploration Studies Office at the Lyndon B. Johnson Space Center, is responsible for coordinating the technical studies necessary for accomplishing such a task. This technical report, produced by the MASE, describes the process that has been developed in a case study approach. The four case studies developed in FY88 include: (1) Human Expedition to Phobos; (2) Human Expedition to Mars; (3) Lunar Observatory; and (4) Lunar Outpost to Early Mars Evolution. The final outcome of this effort is a set of programmatic and technical conclusions and recommendations for the following year's work.
NASA Technical Reports Server (NTRS)
Barclay, Rebecca O.; Pinelli, Thomas E.; Flammia, Madelyn; Kennedy, John M.
1994-01-01
Until the recent dissolution of the Soviet Union, the Communist Party exerted a strict control of access to and dissemination of scientific and technical information (STI). This article presents models of the Soviet-style information society and the Western-style information society and discusses the effects of centralized governmental control of information on Russian technical communication practices. The effects of political control on technical communication are then used to interpret the results of a survey of Russian and U.S. aerospace engineers and scientists concerning the time devoted to technical communication, their collaborative writing practices and their attitudes toward collaboration, the kinds of technical documents they produce and use, and their use of computer technology, and their use of and the importance to them of libraries and technical information centers. The data are discussed in terms of tentative conclusions drawn from the literature. Finally, we conclude with four questions concerning government policy, collaboration, and the flow of STI between Russian and U.S. aerospace engineers and scientists.
Admiralty Inlet Pilot Tidal Project Final Technical Report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Collar, Craig
This document represents the final report for the Admiralty Inlet Pilot Tidal Project, located in Puget Sound, Washington, United States. The Project purpose was to license, permit, and install a grid-connected deep-water tidal turbine array (two turbines) to be used as a platform to gather operational and environmental data on tidal energy generation. The data could then be used to better inform the viability of commercial tidal energy generation from technical, economic, social, and environmental standpoints. This data would serve as a critical step towards the responsible advancement of commercial scale tidal energy in the United States and around themore » world. In late 2014, Project activities were discontinued due to escalating costs, and the DOE award was terminated in early 2015. Permitting, licensing, and engineering design activities were completed under this award. Final design, deployment, operation, and monitoring were not completed. This report discusses the results and accomplishments achieved under the subject award.« less
Rice proteome database: a step toward functional analysis of the rice genome.
Komatsu, Setsuko
2005-09-01
The technique of proteome analysis using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has the power to monitor global changes that occur in the protein complement of tissues and subcellular compartments. In this study, the proteins of rice were cataloged, a rice proteome database was constructed, and a functional characterization of some of the identified proteins was undertaken. Proteins extracted from various tissues and subcellular compartments in rice were separated by 2D-PAGE and an image analyzer was used to construct a display of the proteins. The Rice Proteome Database contains 23 reference maps based on 2D-PAGE of proteins from various rice tissues and subcellular compartments. These reference maps comprise 13129 identified proteins, and the amino acid sequences of 5092 proteins are entered in the database. Major proteins involved in growth or stress responses were identified using the proteome approach. Some of these proteins, including a beta-tubulin, calreticulin, and ribulose-1,5-bisphosphate carboxylase/oxygenase activase in rice, have unexpected functions. The information obtained from the Rice Proteome Database will aid in cloning the genes for and predicting the function of unknown proteins.
Protein phosphorylation differs significantly among ontogenetic phases in Malus seedlings
2014-01-01
Background Although protein phosphorylation is an important post-translational modification affecting protein function and metabolism, dynamic changes in this process during ontogenesis remain unexplored in woody angiosperms. Methods Phosphorylated proteins from leaves of three apple seedlings at juvenile, adult vegetative and reproductive stages were extracted and subjected to alkaline phosphatase pre-treatment. After separating the proteins by two-dimensional gel electrophoresis and phosphoprotein-specific Pro-Q Diamond staining, differentially expressed phosphoproteins were identified by MALDI-TOF-TOF mass spectrometry. Results A total of 107 phosphorylated protein spots on nine gels (three ontogenetic phases × three seedlings) were identified by MALDI-TOF-TOF mass spectrometry. The 55 spots of ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) large-chain fragments varied significantly in protein abundance and degree of phosphorylation among ontogenetic phases. Abundances of the 27 spots corresponding to Rubisco activase declined between juvenile and reproductive phases. More extensively, phosphorylated β-tubulin chain spots with lower isoelectric points were most abundant during juvenile and adult vegetative phases. Conclusions Protein phosphorylation varied significantly during vegetative phase change and floral transition in apple seedlings. Most of the observed changes were consistent among seedlings and between hybrid populations. PMID:24904238
El Rabey, Haddad A; Al-Malki, Abdulrahman L; Abulnaja, Khalid O; Rohde, Wolfgang
2015-01-01
This study was carried out to study the proteome of date palm under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. For this purpose, three-month-old seedlings of date palm cultivar "Sagie" were subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for one month. DIGE analysis of protein extracts identified 47 differentially expressed proteins in leaves of salt- and drought-treated palm seedlings. Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants. The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions. Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Molnar, Denes
2014-04-14
The Section below summarizes research activities and achievements during the first four years of the PI’s Early Career Research Project (ECRP). Two main areas have been advanced: i) radiative 3 ↔ 2 radiative transport, via development of a new computer code MPC/Grid that solves the Boltzmann transport equation in full 6+1D (3X+3V+time) on both single-CPU and parallel computers; ii) development of a self-consistent framework to convert viscous fluids to particles, and application of this framework to relativistic heavy-ion collisions, in particular, determination of the shear viscosity. Year 5 of the ECRP is under a separate award number, and therefore itmore » has its own report document ’Final Technical Report for Year 5 of the Early Career Research Project “Viscosity and equation of state of hot and dense QCDmatter”’ (award DE-SC0008028). The PI’s group was also part of the DOE JET Topical Collaboration, a multi-institution project that overlapped in time significantly with the ECRP. Purdue achievements as part of the JET Topical Collaboration are in a separate report “Final Technical Report summarizing Purdue research activities as part of the DOE JET Topical Collaboration” (award DE-SC0004077).« less
Navajo Electrification Demonstraiton Project
DOE Office of Scientific and Technical Information (OSTI.GOV)
Larry Ahasteen, Project Manager
2006-07-17
This is a final technical report required by DOE for the Navajo Electrification Demonstration Program, This report covers the electric line extension project for Navajo families that currently without electric power.
Cattail-to-alcohol project. Final technical report
DOE Office of Scientific and Technical Information (OSTI.GOV)
None
1982-01-01
Harvesting, grinding, and fermentation of cattails and/or their rhizomes are described. The use of antibiotics to prevent massive contamination of microorganisms and cessation of fermentation is discussed.
National Training Center (NTC) Analysis
1981-05-29
CONTRACT FOR S100K NTC CONCEPT STUDY PRESENTATION TO DARPA/TRADOC SIOOK ADD- ON FOR FURTHER NTC CONCEPT DEVELOPMENT SAI CONCEPT FOR 1-ALPHA...Technical Report 29 May 1981 I I I This document is submitted in fulfillment of contract No. DAAK 40-78-C-0198. It is the final report on Phase 1...many technical reports have been published previously. This report draws on these earlier reports and presents a comprehensive summary and overview
ERIC Educational Resources Information Center
Pinelli, Thomas E.; And Others
As Phase I of a comprehensive evaluation of the NASA-affiliated Langley Research Center's (LaRC) scientific and technical information (STI) program, an internal survey was conducted to obtain feedback from LaRC scientists and engineers concerning the effectiveness of the STI program. The first stage of the survey, which involved interviews with 64…
ERIC Educational Resources Information Center
Pinelli, Thomas E.; And Others
As Phase IV of a comprehensive evaluation of the NASA-affiliated Langley Research Center's (LaRC) scientific and technical information (STI) program, a study was conducted to assess the usage, importance, and perceived quality of Langley-generated STI among academic and industrial research personnel, and to determine ways in which that information…
1999-10-01
Technical Report 5-20448 & 5- 20449 Contract No. DAAH01-98-D-R001 Delivery Order No. 34 Microelectronics Status Analysis and Secondary Part...Procureability Assessment of the THAAD Weapon System. (5-20448 & 5- 20449 ) Final Technical Report for Period 21 January 1999 through 30 September 1999...Huntsville Huntsville, AL 35899 5. FUNDING NUMBERS 8. PERFORMING ORGANIZATION REPORT NUMBER 5-20448 & 5- 20449 9. SPONSORING/MONITORING AGENCY
Final Technical Report: "Achieving Regional Energy Efficiency Potential in the Southeast”
DOE Office of Scientific and Technical Information (OSTI.GOV)
Mahoney, Mandy
The overall objective of this award was to facilitate sharing of DOE resources and best practices as well as provide technical assistance to key stakeholders to support greater compliance with energy efficiency standards and increased energy savings. The outcomes of this award include greater awareness among key stakeholders on energy efficiency topics, increased deployment and utilization of DOE resources, and effective policies and programs to support energy efficiency in the Southeast.
2008-08-01
and sponsoring FIRST teams at the Junior FIRST Lego League (JFLL) and the FIRST Lego League (FLL) levels in elementary and middle school . As such, the...a Final Technical Report for Year I Grant W911NF-07-1-0663 with the Newport News Public School District for the Menchville High School Robotics Team...August 2008 TRIPL HELIX 0 Menchville High School Newport News, VA 20080829081 w DEFENSE TECHNICAL INFORMATION CENTER bfro do.A icfr tkw, Def&*ue, Cow
Review of Interventions for Reducing Enlisted Attrition in the U.S. Military: An Update
2009-06-01
by ARI. Please address correspondence concerning distribution of reports to: U.S. Research Institute for the Behavioral and Social Sciences, Attn...DAPE-ARI-ZXM, 2511 Jefferson Davis Highway, Arlington, Virginia 22202-3926 FINAL DISPOSITION: This Technical Report may be destroyed when it...this Technical Report are not to be construed as an official Department of the Army position, unless so designated by other authorized documents. i
Navy Technical Information Presentation System (NTIPS) Test and Implementation Strategy
1981-12-01
IC AROEROCK I NAOI S ~ i P RF R M N C AVI AT OIO N A N DDEPARTMENT STIPRUCTRMNES COMPUATIONAN DEPARTMENT -MATHEMATICS AND 17 LOGISTICS DEPARTMENT leI...and Subtitle) S . TYPE OF REPORT & PERIOD COVERED NAVY TECHNICAL INFORMATION PRESENTATION Final SYSTEM (NTIPS) TEST AND IMPLEMENTATION 6. PERFORMING...CLASSIFICATION OP THIS PAGE (1nor. Data Enteed) ock 20 continued) system operation, training, maintenance, and logistics support. This system was
1993-03-01
1993 A Final Technical Report B 0 RF A Approved for public release; distribution is unlimited. T 0 R 93-12309;.801 01 7 lllli/liillU!ll11!tl AIR FORCE... publication is for illustration purposes and does not constitute endorsement or recommendaticn for usc by th• United States Air Force. The Office of... Public Affairs has reviewed this report, and it is releasable to the National Technical Information Service, where it will be available to the general
From embodied mind to embodied robotics: humanities and system theoretical aspects.
Mainzer, Klaus
2009-01-01
After an introduction (1) the article analyzes the evolution of the embodied mind (2), the innovation of embodied robotics (3), and finally discusses conclusions of embodied robotics for human responsibility (4). Considering the evolution of the embodied mind (2), we start with an introduction of complex systems and nonlinear dynamics (2.1), apply this approach to neural self-organization (2.2), distinguish degrees of complexity of the brain (2.3), explain the emergence of cognitive states by complex systems dynamics (2.4), and discuss criteria for modeling the brain as complex nonlinear system (2.5). The innovation of embodied robotics (3) is a challenge of future technology. We start with the distinction of symbolic and embodied AI (3.1) and explain embodied robots as dynamical systems (3.2). Self-organization needs self-control of technical systems (3.3). Cellular neural networks (CNN) are an example of self-organizing technical systems offering new avenues for neurobionics (3.4). In general, technical neural networks support different kinds of learning robots (3.5). Finally, embodied robotics aim at the development of cognitive and conscious robots (3.6).
NASA Technical Reports Server (NTRS)
McCurry, J.
1995-01-01
The purpose of the TA-2 contract was to provide advanced launch vehicle concept definition and analysis to assist NASA in the identification of future launch vehicle requirements. Contracted analysis activities included vehicle sizing and performance analysis, subsystem concept definition, propulsion subsystem definition (foreign and domestic), ground operations and facilities analysis, and life cycle cost estimation. This document is part of the final report for the TA-2 contract. The final report consists of three volumes: Volume 1 is the Executive Summary, Volume 2 is Technical Results, and Volume 3 is Program Cost Estimates. The document-at-hand, Volume 1, provides a summary description of the technical activities that were performed over the entire contract duration, covering three distinct launch vehicle definition activities: heavy-lift (300,000 pounds injected mass to low Earth orbit) launch vehicles for the First Lunar Outpost (FLO), medium-lift (50,000-80,000 pounds injected mass to low Earth orbit) launch vehicles, and single-stage-to-orbit (SSTO) launch vehicles (25,000 pounds injected mass to a Space Station orbit).
Fayyaz Khan, Humaira; Farooq Danish, Khalid; Saeed Awan, Azra; Anwar, Masood
2013-05-01
The purpose of the study was to identify technical item flaws in the multiple choice questions submitted for the final exams for the years 2009, 2010 and 2011. This descriptive analytical study was carried out in Islamic International Medical College (IIMC). The Data was collected from the MCQ's submitted by the faculty for the final exams for the year 2009, 2010 and 2011. The data was compiled and evaluated by a three member assessment committee. The data was analyzed for frequency and percentages the categorical data was analyzed by chi-square test. Overall percentage of flawed item was 67% for the year 2009 of which 21% were for testwiseness and 40% were for irrelevant difficulty. In year 2010 the total item flaws were 36% and 11% testwiseness and 22% were for irrelevant difficulty. The year 2011 data showed decreased overall flaws of 21%. The flaws of testwisness were 7%, irrelevant difficulty were 11%. Technical item flaws are frequently encountered during MCQ construction, and the identification of flaws leads to improved quality of the single best MCQ's.
Advanced High Temperature Polymer Matrix Composites for Gas Turbine Engines Program Expansion
NASA Technical Reports Server (NTRS)
Hanley, David; Carella, John
1999-01-01
This document, submitted by AlliedSignal Engines (AE), a division of AlliedSignal Aerospace Company, presents the program final report for the Advanced High Temperature Polymer Matrix Composites for Gas Turbine Engines Program Expansion in compliance with data requirements in the statement of work, Contract No. NAS3-97003. This document includes: 1 -Technical Summary: a) Component Design, b) Manufacturing Process Selection, c) Vendor Selection, and d) Testing Validation: 2-Program Conclusion and Perspective. Also, see the Appendix at the back of this report. This report covers the program accomplishments from December 1, 1996, to August 24, 1998. The Advanced High Temperature PMC's for Gas Turbine Engines Program Expansion was a one year long, five task technical effort aimed at designing, fabricating and testing a turbine engine component using NASA's high temperature resin system AMB-21. The fiber material chosen was graphite T650-35, 3K, 8HS with UC-309 sizing. The first four tasks included component design and manufacturing, process selection, vendor selection, component fabrication and validation testing. The final task involved monthly financial and technical reports.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Tagestad, Jerry D.; Bolte, John; Guzy, Michael
During this final year of the Pacific Northwest Regional Collaboratory we focused significantly on continuing the relationship between technical teams and government end-users. The main theme of the year was integration. This took the form of data integration via our web portal and integration of our technologies with the end users. The PNWRC's technical portfolio is based on EOS strategies, and focuses on 'applications of national priority: water management, invasive species, coastal management and ecological forecasting.' The products of our technical approaches have been well received by the community of focused end-users. The objective this year was to broaden thatmore » community and develop external support to continue and operationalize product development.« less
Diverse knowledges and competing interests: an essay on socio-technical problem-solving.
di Norcia, Vincent
2002-01-01
Solving complex socio-technical problems, this paper claims, involves diverse knowledges (cognitive diversity), competing interests (social diversity), and pragmatism. To explain this view, this paper first explores two different cases: Canadian pulp and paper mill pollution and siting nuclear reactors in systematically sensitive areas of California. Solving such socio-technically complex problems involves cognitive diversity as well as social diversity and pragmatism. Cognitive diversity requires one to not only recognize relevant knowledges but also to assess their validity. Finally, it is suggested, integrating the resultant set of diverse relevant and valid knowledges determines the parameters of the solution space for the problem.
ERIC Educational Resources Information Center
McKinney, Floyd L., Ed.; Sims, E. Norman, Ed.
This book contains presentations made at a series of 3-day workshops held in Kentucky to prepare vocational educators to write proposals, progress reports, and final reports for research and development efforts. Workshop presentation included "Need for Research and Development in Vocational-Technical Education," by Robert Warmbrod,…
Research on Damage Models for Continuous Fiber Composites
1988-07-01
r ~.F (~ Mechanics and Materials Center TEXAS A&M UNIVERSITY College Station, Texas RESEARCH ON DAMAGE MODELS FOR CONTINUOUS FIBER COMPOSITES Final...Washington, DC 20332 11. TITLE (Include Security Clas=fication) Research on Damage Models for Continuous Fiber Composites - Final Technical Report 1...GROUP SUB-GROU ::=, COMPOsites ) continuum mechanics , ~ idamage, internal state variables V experimental mechanics, laminated composites o 19. ABSTRACT
ERIC Educational Resources Information Center
Brown, DiAnn; Gaar, Sara J.
This final report describes activities and accomplishments of Alaska's 3-year program on services for children and youth with dual sensory impairments. The project provided technical assistance to local education agencies, infant learning programs, and other educators. It also provided support and training to families of children with…
ERIC Educational Resources Information Center
Carman, Priscilla S.
These two documents are products of a project to improve the capability of the Pennsylvania Regional Staff Development Centers to provide current, research-based workplace literacy training and technical assistance to adult basic and literacy education programs. The final report describes these project activities: development of training materials…
ERIC Educational Resources Information Center
Stenner, A. Jackson; And Others
This document contains the second of five volumes reporting the activities and results of a career education evaluation project conducted to accomplish the following two objectives: (1) to improve the quality of evaluations by career education projects funded by the United States Office of Career Education (OCE) through the provision of technical…
DOE Office of Scientific and Technical Information (OSTI.GOV)
Rich Schiferl
2008-05-30
High temperature superconducting (HTS) motors offer the potential for dramatic volume and loss reduction compared to conventional, high horspower, industrial motors. This report is the final report on the results of eight research tasks that address some of the issues related to HTS motor development that affect motor efficiency, cost, and reliability.
ERIC Educational Resources Information Center
Stremel, Kathleen
This final report discusses the activities and outcomes of a project designed to assist states in improving the quality of existing placements and services for individuals (birth through young adulthood) who are deaf-blind, and to increase the number of children, their families, and their service providers who will benefit from these services. The…
Research in Information Processing and Computer Science. Final Technical Report.
ERIC Educational Resources Information Center
Carnegie-Mellon Univ., Pittsburgh, PA. Social Studies Curriculum Center.
This is the final scientific research report for the research in programing at Carnegie-Mellon University during 1968-1970. Three team programing efforts during the past two years have been the development of (1) BLISS--a system building language on the PDP-10 computer, (2) LC2--a conversational system on the IBM/360, and L*--a system building…
ERIC Educational Resources Information Center
Wickey, Jane M.; Hartman, Barbara L.
Presented is the final report of a 1-year project designed to determine the possibility of coordinating services for handicapped children in the Baltimore (Maryland) region by providing technical assistance to coalitions of decision makers at the local and regional levels. Some of the findings and conclusions drawn from the project are listed such…
On March 24, 1993 EPA finalized a new test procedure to measure evaporative emissions from motor vehicles. The amendments modify several of the test procedure’s tolerances, equipment specifications, and procedural steps.
An Investigation of Spoken Brazilian Portuguese: Part I, Technical Report. Final Report.
ERIC Educational Resources Information Center
Hutchins, John A.
This final report of a study which developed a working corpus of spoken and written Portuguese from which syntactical studies could be conducted includes computer-processed data on which the findings and analysis are based. A data base, obtained by taping some 487 conversations between Brazil and the United States, serves as the corpus from which…
Fifth SIAM conference on geometric design 97: Final program and abstracts. Final technical report
DOE Office of Scientific and Technical Information (OSTI.GOV)
NONE
1997-12-31
The meeting was divided into the following sessions: (1) CAD/CAM; (2) Curve/Surface Design; (3) Geometric Algorithms; (4) Multiresolution Methods; (5) Robotics; (6) Solid Modeling; and (7) Visualization. This report contains the abstracts of papers presented at the meeting. Proceding the conference there was a short course entitled ``Wavelets for Geometric Modeling and Computer Graphics``.
2006-03-01
AFRL-IF-RS-TR-2006-88 Final Technical Report March 2006 MONITORING BUSINESS ACTIVITY New York University...REPORT DATE MARCH 2006 3. REPORT TYPE AND DATES COVERED Final Sep 01 – Oct 05 4. TITLE AND SUBTITLE MONITORING BUSINESS ACTIVITY 6. AUTHOR(S...Accepted to Journal of Machine Learning Research, pending revisions. CeDER Working Paper #CeDER-04-08, Stern School of Business , New York University
2014-11-06
This final rule will update and make revisions to the End-Stage Renal Disease (ESRD) prospective payment system (PPS) for calendar year (CY) 2015. This rule also finalizes requirements for the ESRD quality incentive program (QIP), including for payment years (PYs) 2017 and 2018. This rule will also make a technical correction to remove outdated terms and definitions. In addition, this final rule sets forth the methodology for adjusting Durable Medical Equipment, Prosthetics, Orthotics, and Supplies (DMEPOS) fee schedule payment amounts using information from the Medicare DMEPOS Competitive Bidding Program (CBP); makes alternative payment rules for certain DME under the Medicare DMEPOS CBP; clarifies the statutory Medicare hearing aid coverage exclusion and specifies devices not subject to the hearing aid exclusion; will not update the definition of minimal self-adjustment; clarifies the Change of Ownership (CHOW) and provides for an exception to the current requirements; revises the appeal provisions for termination of a CBP contract, including the beneficiary notification requirement under the Medicare DMEPOS CBP, and makes a technical change to the regulation related to the conditions for awarding contracts for furnishing infusion drugs under the Medicare DMEPOS CBP.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Kreutz, Thomas G; Ogden, Joan M
2000-07-01
In the final report, we present results from a technical and economic assessment of residential scale PEM fuel cell power systems. The objectives of our study are to conceptually design an inexpensive, small-scale PEMFC-based stationary power system that converts natural gas to both electricity and heat, and then to analyze the prospective performance and economics of various system configurations. We developed computer models for residential scale PEMFC cogeneration systems to compare various system designs (e.g., steam reforming vs. partial oxidation, compressed vs. atmospheric pressure, etc.) and determine the most technically and economically attractive system configurations at various scales (e.g., singlemore » family, residential, multi-dwelling, neighborhood).« less
Advanced Tools and Techniques for Formal Techniques in Aerospace Systems
NASA Technical Reports Server (NTRS)
Knight, John C.
2005-01-01
This is the final technical report for grant number NAG-1-02101. The title of this grant was "Advanced Tools and Techniques for Formal Techniques In Aerospace Systems". The principal investigator on this grant was Dr. John C. Knight of the Computer Science Department, University of Virginia, Charlottesville, Virginia 22904-4740. This report summarizes activities under the grant during the period 7/01/2002 to 9/30/2004. This report is organized as follows. In section 2, the technical background of the grant is summarized. Section 3 lists accomplishments and section 4 lists students funded under the grant. In section 5, we present a list of presentations given at various academic and research institutions about the research conducted. Finally, a list of publications generated under this grant is included in section 6.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Burgess, Dave
2003-11-01
This annual report is a precursor to the final technical report we will be writing the next contract period. Consequently, this report, covering the period between September 27, 2002, and September 26, 2003, represents a progress report towards the final technical report we anticipate completing by September 26, 2004. Sample analysis and field work have progressed well and we anticipate no further delays. There are 4 objectives: (1) To quantify secondary production Moses Lake; (2) To quantify the influence of predation on target fishes in Moses Lake; (3) To quantify mortality of selected fished in Moses Lake; and (4) Tomore » assess effects of habitat changes from shoreline development and carp on the fish community in Moses Lake.« less
1989-07-01
to the FINAL ENVIRONMENTAL IMPACT STATEMENT D T C EIECTE SMAR 1 41990 US Army Corps of Engineers Rock Island District JULY 1989 S03 1601...SUPPLEMENT NO. 1 TO THE FINAL ENVIRONMENTAL IMPACT STATEMENT NTIS z ITIC Av’, < , r Dist JULY 1989 TECHNICAL CONTRIBUTORS Primary study team personnel who are...AND COMMENTS 45 SECTION 6 - RECOMMENDATIONS 46 List of Tables No. Title Page 1 Impacts of Levee on Raccoon River Profiles 20 2 Impacts of Levee on
1985-05-13
The Food and Drug Administration (FDA) is announcing the availability of final recommendations prepared by its Center for Devices and Radiological Health (CDRH) on quality assurance programs in nuclear medicine facilities. The final recommendations include the agency's rationale for the recommendations as well as references that can be used as well as references that can be used as guides in conducting quality control monitoring. These final recommendations are available as a technical report in CDRH's radiation recommendations series. They are intended to encourage and promote the development of voluntary quality assurance programs in nuclear medicine facilities.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Boing, L.E.
This paper presents an overview of the US experiences in the decommissioning technical area. Sections included are: (1) an overview of the magnitude of the problem, (2) a review of the US decommissioning process, (3) regulation of decommissioning, (4) regulatory and funding requirements for decommissioning, and (5) a general overview of all on-going and completed decommissioning projects to date in the US. The final section presents a review of some issues in the decommissioning area currently being debated in the technical specialists community.
Flora of the Fraser Experimental Forest, Colorado. Forest Service general technical report (Final)
DOE Office of Scientific and Technical Information (OSTI.GOV)
Popovich, S.J.; Shepperd, W.D.; Reichert, D.W.
1993-08-01
The report lists 441 vascular plant taxa in 228 genera and 63 families encountered on the 9,300-ha Fraser Experimental Forest in central Colorado. Synonyms appearing in previous publications and other works pertaining to the Fraser Experimental Forest, as well as appropriate Colorado floras and less-technical field guides, are included. Plant communities and habitats are discussed, and a list of 54 lichens is also presented. A glossary of related terms is included.
Goal Reconstruction: How Teton Blends Situated Action and Planned Action
1989-11-03
DTIC FILE COPy 00 P 1n GOAL RECONSTRUCTION: HOW TETON BLENDS N SITUATED ACTION AND PLANNED ACTION Technical Report AIP 125 Kurt VanLehn William Ball...distribiikn unlimited. so 08 21 2 GOAL RECONSTRUCTION: HOW TETON BLENDS SITUATED ACTION AND PLANNED ACTION Technical Report AlP 125 Kurt VanLehn William Ball...Architectures for Intelligence. This is the final report on the research supported by the Computer Science Division, Office of Naval Research, under
National Alliance for Advanced Biofuels and Bio-Products Final Technical Report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Olivares, Jose A.; Baxter, Ivan; Brown, Judith
2014-09-30
The main objective of NAABB was to combine science, technology, and engineering expertise from across the nation to break down critical technical barriers to commercialization of algae-based biofuels. The approach was to address technology development across the entire value chain of algal biofuels production, from selection of strains to cultivation, harvesting, extraction, fuel conversion, and agricultural coproduct production. Sustainable practices and financial feasibility assessments ununderscored the approach and drove the technology development.
ERIC Educational Resources Information Center
Louisiana State Dept. of Education, Baton Rouge. Div. of Vocational Education.
The curriculum guide for air conditioning/refrigeration is one of five guides written and field tested in a project to develop statewide articulated competency-based curricula in selected vocational education programs. Two separate curricula, one for the vocational-technical level and one for the associate degree level, are presented. The six…
PVWatts Version 1 Technical Reference
DOE Office of Scientific and Technical Information (OSTI.GOV)
Dobos, A. P.
2013-10-01
The NREL PVWatts(TM) calculator is a web application developed by the National Renewable Energy Laboratory (NREL) that estimates the electricity production of a grid-connected photovoltaic system based on a few simple inputs. PVWatts combines a number of sub-models to predict overall system performance, and makes several hidden assumptions about performance parameters. This technical reference details the individual sub-models, documents assumptions and hidden parameters, and explains the sequence of calculations that yield the final system performance estimation.
Technical Issues in Evolving to Integrated Services Digital Network (ISDN)
1991-06-01
channel through some Page 13 Technical Issues in Evolving to ISDN Final Report separate interface (such as the AT command set of the Hayes modems or the...errors experienced over standard modem provided connectivity. But, in this project connectivity has been established only over a single CO. Those...examined to some extent and are discussed below. Existing equipment was of two types: that which treats ISDN as just another leased line providing 56k or
2012-07-01
Incremental Validity of Biographical Data in the Prediction of En Route Air Traffic Control Specialist Technical Skills Dana Broach Civil Aerospace...Medical Institute Federal Aviation Administration Oklahoma City, OK 73125 July 2012 Final Report DOT/FAA/AM- 12 /8 Office of Aerospace Medicine...FAA/AM- 12 /8 4. Title and Subtitle 5. Report Date July 2012 Incremental Validity of Biographical Data in the Prediction of En Route Air
Fullerene data mining using bibliometrics and database tomography
Kostoff; Braun; Schubert; Toothman; Humenik
2000-01-01
Database tomography (DT) is a textual database analysis system consisting of two major components: (1) algorithms for extracting multiword phrase frequencies and phrase proximities (physical closeness of the multiword technical phrases) from any type of large textual database, to augment (2) interpretative capabilities of the expert human analyst. DT was used to derive technical intelligence from a fullerenes database derived from the Science Citation Index and the Engineering Compendex. Phrase frequency analysis by the technical domain experts provided the pervasive technical themes of the fullerenes database, and phrase proximity analysis provided the relationships among the pervasive technical themes. Bibliometric analysis of the fullerenes literature supplemented the DT results with author/journal/institution publication and citation data. Comparisons of fullerenes results with past analyses of similarly structured near-earth space, chemistry, hypersonic/supersonic flow, aircraft, and ship hydrodynamics databases are made. One important finding is that many of the normalized bibliometric distribution functions are extremely consistent across these diverse technical domains and could reasonably be expected to apply to broader chemical topics than fullerenes that span multiple structural classes. Finally, lessons learned about integrating the technical domain experts with the data mining tools are presented.
Coalition Battle Management Language (C-BML) (Language de gestion du champ de bataille (C-BML)
2012-02-01
de gestion du champ de bataille (C-BML)) NMSG-048 Final Report. This document...BML) (Langage de gestion du champ de bataille (C-BML)) NMSG-048 Final Report. This document contains information authorized by NATO RTA for...Technical Readiness Level (TRL) that is consistent with an operational deployment. ES - 2 RTO-TR-MSG-048 Langage de gestion du champ de
Analytical Ferrography Standardization.
1982-01-01
AD-AII6 508 MECHANICAL TECHNOLOGY INC LATHAM NY RESEARCH AND 0EV--ETC F/6 7/4 ANALYTICAL FERROGRAPHY STANDARDIZATION. (U) JAN 82 P A SENHOLZI, A S...ii Mwl jutio7 Unimte SMechanical Technology Incorporated Research and Development Division ReerhadDvlpetDvso I FINAL REPORT ANALYTICAL FERROGRAPHY ...Final Report MTI Technical Report No. 82TRS6 ANALYTICAL FERROGRAPHY STANDARDIZATION P. B. Senholzi A. S. Maciejewski Applications Engineering Mechanical
DOE Office of Scientific and Technical Information (OSTI.GOV)
Not Available
The Environmental Engineering Committee conducted a preliminary review of the above document in March 1986, and identified obvious errors or omissions which are explained in detail in its initial report. The Office of Solid Waste asked the Committee to review the final draft ACL guidance when it was ready for publications in the Federal Register. This report represents the Committee's review of the final draft which was found to be well-written and technically sound.
Ontologies, Knowledge Bases and Knowledge Management
2002-07-01
AFRL-IF-RS-TR-2002-163 Final Technical Report July 2002 ONTOLOGIES, KNOWLEDGE BASES AND KNOWLEDGE MANAGEMENT USC Information ...and layer additional information necessary to make specific uses of the knowledge in this core. Finally, while we were able to find adequate solutions... knowledge base and inference engine. Figure 3.2: SDA Editor Interface 46 Although the SDA has access to information about the situation, we wanted the user
Technical-tactical analysis of youth olympic taekwondo combat.
Tornello, Francesco; Capranica, Laura; Minganti, Carlo; Chiodo, Salvatore; Condello, Giancarlo; Tessitore, Antonio
2014-04-01
The purpose of this study was to define the technical and tactical profiles of official youth taekwondo competitions played under the most recent rules of the International Taekwondo Federation. Tactical actions (i.e., attack, defense, and block), technical executions (from 1- to 4-point scores), kicking legs (i.e., front/rear and right/left), and overall technical effectiveness were investigated in relation to match outcome of semifinal and final competitions (n = 50) of youth (aged 13-14 years) black belt athletes during the Italian Taekwondo Cadet Championship. Differences (p < 0.001) were found among all action typologies (Attack: 50.9 ± 2.2%; Defense: 27.7 ± 1.5%; Block: 21.3 ± 1.6%), with winners showing fewer (p = 0.005) offensive actions and more (p = 0.001) defensive actions with respect to non-winners. Independently from match outcome, technical exchanges showed differences (p < 0.001) for technical executions. Winners resulted more efficient (p < 0.001) for both technical and tactical variables. In general, these findings showed that Cadets tend to adopt an offensive strategy. In considering that the adoption of the new electronic system requires athletes to execute correct technical actions to have a score assigned, coaches should emphasize the effectiveness of scoring techniques and help athletes to effectively improve their defense and counterattack capabilities.
Pioneer women in engineering studies: what can we learn from their experiences?
NASA Astrophysics Data System (ADS)
Pardo, P.; Calvet, M. D.; Pons, O.; Martinez, M. C.
2016-11-01
Women's access to higher education in Spain began in the last third of the nineteenth century. However, the full incorporation of women into technical studies did not occur until a century later. This article presents the results of research into the access of the first women to do technical studies in Catalonia (northeast Spain). Data have been collected from 11 technical schools belonging to the Universitat Politècnica de Catalunya (UPC) with the aim of understanding the dynamics of the incorporation of women into these schools between 1851 (foundation of the first engineering school in Catalonia) and 1980. Interviews were conducted with 21 pioneer women who completed their technical studies. Their experiences show how technical schools had to adapt, both physically and culturally, to female students. Finally, the current status of female engineers in Catalonia is compared with the situation in other Western countries. Similarities are found that show the relevance of gender as a social structuring force.
Energy changes in transforming solids. Final technical report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Herrmann, G.
Research is reported on energy changes in transforming solids. Topics include: damage mechanics, functionally gradient materials with defects, problems in heterogenization, and conservation laws with application to fracture mechanics and defect mechanics.
48 CFR 235.072 - Additional contract clauses.
Code of Federal Regulations, 2014 CFR
2014-10-01
... contracts for research and development. (d) Use the clause at 252.235-7011, Final Scientific or Technical Report, in solicitations and contracts for research and development. (e) Use the clause at 252.235-7004...
DOE Office of Scientific and Technical Information (OSTI.GOV)
Locke, G.L.
1958-09-08
The design basis is composed of requirements and conditions for the design of the reactor plant (composed of the reactor and heat dissipation system). Its intent is to insure that the final product meets the economic, safety, and technical objectives of the project. The design basis is dependent on the ground rules, objectives, technical criteria, and practical design considerations. This document is being issued with the understanding that these items are not yet firmly established in all respects, and therefore, the numbers put down here are subject to change. Consideration of the spectrum of probable changes that might be mademore » leads to the conclusion that the numbers here are close to the final ones and are satisfactory as a basis for the initial stages of design. Some numbers are omitted because of insufficient data at this time.« less
DOE Office of Scientific and Technical Information (OSTI.GOV)
O'Farrell, T.; Hund, F.
1986-12-01
The document presents the technical rationale for best conventional technology (BCI) effluent limitations guidelines for the pharmaceutical manufacturing point-source category as required by the Clean Water Act of 1977 (P.L. 95-217, the Act). The document describes the technologies considered as the bases for BCT limitations. Section II of this document summarizes the rulemaking process. Sections III through V describe the technical data and engineering analyses used to develop the regulatory technology options. The costs and removals associated with each technology option for each plant and the application of the BCT cost test methodology are presented in Section VI. BCI limitationsmore » bases on the best conventional pollutant control technology are to be achieved by existing direct-discharging facilities.« less
Final Technical Report for SISGR: Ultrafast Molecular Scale Chemical Imaging
DOE Office of Scientific and Technical Information (OSTI.GOV)
Hersam, Mark C.; Guest, Jeffrey R.; Guisinger, Nathan P.
2017-04-10
The Northwestern-Argonne SISGR program utilized newly developed instrumentation and techniques including integrated ultra-high vacuum tip-enhanced Raman spectroscopy/scanning tunneling microscopy (UHV-TERS/STM) and surface-enhanced femtosecond stimulated Raman scattering (SE-FSRS) to advance the spatial and temporal resolution of chemical imaging for the study of photoinduced dynamics of molecules on plasmonically active surfaces. An accompanying theory program addressed modeling of charge transfer processes using constrained density functional theory (DFT) in addition to modeling of SE-FSRS, thereby providing a detailed description of the excited state dynamics. This interdisciplinary and highly collaborative research resulted in 62 publications with ~ 48% of them being co-authored by multiplemore » SISGR team members. A summary of the scientific accomplishments from this SISGR program is provided in this final technical report.« less
Murray, Kara; McKenzie, Karen; Kelleher, Michael
2016-10-01
The importance of non-technical skills (NTS) to patient outcomes is increasingly being recognised, however, there is limited research into how such skills can be taught and evaluated in student nurses in relation toward rounds. This pilot study describes an evaluation of a NTS framework that could potentially be used to measure ward round skills of student nurses. The study used an observational design. Potential key NTS were identified from existing literature and NTS taxonomies. The proposed framework was then used to evaluate whether the identified NTS were evident in a series of ward round simulations that final year general nursing students undertook as part of their training. Finally, the views of a small group of qualified nurse educators, qualified nurses and general nursing students were sought about whether the identified NTS were important and relevant to practice. The proposed NTS framework included seven categories: Communication, Decision Making, Situational Awareness, Teamwork and Task Management, Student Initiative and Responsiveness to Patient. All were rated as important and relevant to practice. The pilot study suggests that the proposed NTS framework could be used as a means of evaluating student nurse competencies in respect of many non-technical skills required for a successful ward round. Further work is required to establish the validity of the framework in educational settings and to determine the extent to which it is of use in a non-simulated ward round setting. Copyright © 2016 Elsevier Ltd. All rights reserved.
Parameter identification for nonlinear aerodynamic systems
NASA Technical Reports Server (NTRS)
Pearson, Allan E.
1993-01-01
This final technical report covers a three and one-half year period preceding February 28, 1993 during which support was provided under NASA Grant NAG-1-1065. Following a general description of the system identification problem and a brief survey of methods to attack it, the basic ideas behind the approach taken in this research effort are presented. The results obtained are described with reference to the published work, including the five semiannual progress reports previously submitted and two interim technical reports.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Tedeschi, Jonathan R.; Bernacki, Bruce E.; Kelly, James F.
2011-12-31
This report describes research and development efforts toward a novel passive millimeter-wave (mm-wave) electromagnetic imaging device for broad-area search. It addresses the technical challenge of detecting anomalies that occupy a small fraction of a pixel. The purpose of the imager is to pinpoint suspicious locations for cuing subsequent higher-resolution imaging. The technical basis for the approach is to exploit thermal and polarization anomalies that distinguish man-made features from natural features.
1986-11-01
whales , sei whales , sperm whales and leatherback sea turtles within t e inland waters of Puget Sound clearly indicates that the...AL)-A175 114 CARRIER BATTLE GROUP CCVBG) HOKEPORTING In THE PUGET In SOUND AREA WASHINGTON STATE TECHNICAL APPENDICESeu) CORPS OF ENGINEERS SEATTLE...34 , ,’ ’, ’ , % ". ". ", ." , , -,, ". ", . ,,, ’. .. ". -"’. -".’-".’,. Final Supplemental to U.S. Navy/ Environmental Impact Statement Carrier Battle Group
NASA Technical Reports Server (NTRS)
McCurry, J. B.
1995-01-01
The purpose of the TA-2 contract was to provide advanced launch vehicle concept definition and analysis to assist NASA in the identification of future launch vehicle requirements. Contracted analysis activities included vehicle sizing and performance analysis, subsystem concept definition, propulsion subsystem definition (foreign and domestic), ground operations and facilities analysis, and life cycle cost estimation. The basic period of performance of the TA-2 contract was from May 1992 through May 1993. No-cost extensions were exercised on the contract from June 1993 through July 1995. This document is part of the final report for the TA-2 contract. The final report consists of three volumes: Volume 1 is the Executive Summary, Volume 2 is Technical Results, and Volume 3 is Program Cost Estimates. The document-at-hand, Volume 3, provides a work breakdown structure dictionary, user's guide for the parametric life cycle cost estimation tool, and final report developed by ECON, Inc., under subcontract to Lockheed Martin on TA-2 for the analysis of heavy lift launch vehicle concepts.
Technical approach to groundwater restoration. Final report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Not Available
1993-11-01
The Technical Approach to Groundwater Restoration (TAGR) provides general technical guidance to implement the groundwater restoration phase of the Uranium Mill Tailings Remedial Action (UMTRA) Project. The TAGR includes a brief overview of the surface remediation and groundwater restoration phases of the UMTRA Project and describes the regulatory requirements, the National Environmental Policy Act (NEPA) process, and regulatory compliance. A section on program strategy discusses program optimization, the role of risk assessment, the observational approach, strategies for meeting groundwater cleanup standards, and remedial action decision-making. A section on data requirements for groundwater restoration evaluates the data quality objectives (DQO) andmore » minimum data required to implement the options and comply with the standards. A section on sits implementation explores the development of a conceptual site model, approaches to site characterization, development of remedial action alternatives, selection of the groundwater restoration method, and remedial design and implementation in the context of site-specific documentation in the site observational work plan (SOWP) and the remedial action plan (RAP). Finally, the TAGR elaborates on groundwater monitoring necessary to evaluate compliance with the groundwater cleanup standards and protection of human health and the environment, and outlines licensing procedures.« less
Federal Register 2010, 2011, 2012, 2013, 2014
2012-05-29
...This final rule reflects the Centers for Medicare & Medicaid Services' commitment to the general principles of the President's Executive Order 13563 released January 18, 2011, entitled ``Improving Regulation and Regulatory Review.'' This rule will: implement a new reconsideration process for administrative determinations to disallow claims for Federal financial participation (FFP) under title XIX of the Act (Medicaid); lengthen the time States have to credit the Federal government for identified but uncollected Medicaid provider overpayments and provide that interest will be due on amounts not credited within that time period; make conforming changes to the Medicaid and Children's Health Insurance Program (CHIP) disallowance process to allow States the option to retain disputed Federal funds through the new administrative reconsideration process; revise installment repayment standards and schedules for States that owe significant amounts; and provide that interest charges may accrue during the new administrative reconsideration process if a State chooses to retain the funds during that period. This final rule will also make a technical correction to reporting requirements for disproportionate share hospital payments, revise internal delegations of authority to reflect the term ``Administrator or current Designee,'' remove obsolete language, and correct other technical errors.
Surgical Practical Skills Learning Curriculum: Implementation and Interns' Confidence Perceptions.
Acosta, Danilo; Castillo-Angeles, Manuel; Garces-Descovich, Alejandro; Watkins, Ammara A; Gupta, Alok; Critchlow, Jonathan F; Kent, Tara S
To provide an overview of the practical skills learning curriculum and assess its effects over time on the surgical interns' perceptions of their technical skills, patient management, administrative tasks, and knowledge. An 84-hour practical skills curriculum composed of didactic, simulation, and practical sessions was implemented during the 2015 to 2016 academic year for general surgery interns. Totally, 40% of the sessions were held during orientation, whereas the remainder sessions were held throughout the academic year. Interns' perceptions of their technical skills, administrative tasks, patient management, and knowledge were assessed by the practical skills curriculum residents' perception survey at various time points during their intern year (baseline, midpoint, and final). Interns were also asked to fill out an evaluation survey at the completion of each session to obtain feedback on the curriculum. General Surgery Residency program at a tertiary care academic institution. 20 General Surgery categorical and preliminary interns. Significant differences were found over time in interns' perceptions on their technical skills, patient management, administrative tasks, and knowledge (p < 0.001 for all). The results were also statistically significant when accounting for a prior boot camp course in medical school, intern status (categorical or preliminary), and gender (p < 0.05 for all). Differences in interns' perceptions occurred both from baseline to midpoint, and from midpoint to final time point evaluations (p < 0.001 for all). Prior surgical boot camp in medical school status, intern status (categorical vs. preliminary), and gender did not differ in the interns' baseline perceptions of their technical skills, patient management, administrative tasks, and knowledge (p > 0.05 for all). Implementation of a Practical Skills Curriculum in surgical internships can improve interns' confidence perception on their technical skills, patient management skills, administrative tasks, and knowledge. Copyright © 2018. Published by Elsevier Inc.
An Estimation Theory for Differential Equations and other Problems, with Applications.
1981-11-01
order differential -8- operators and M-operators, in particular, the Perron - Frobenius theory and generalizations. Convergence theory for iterative... THEORY FOR DIFFERENTIAL 0EQUATIONS AND OTHER FROBLEMS, WITH APPLICATIONS 0 ,Final Technical Report by Johann Schr6der November, 1981 EUROPEAN RESEARCH...COVERED An estimation theory for differential equations Final Report and other problrms, with app)lications A981 6. PERFORMING ORG. RN,-ORT NUMfFR 7
Final Technical Report for subcontract number B612144
DOE Office of Scientific and Technical Information (OSTI.GOV)
Mayali, X.; Marcu, O.
The original statement of work stipulated that the Subcontractor shall perform bacterial and algal cultivation and manipulation, microbe isolation, preparation of samples for sequencing and isotopic analysis, data analysis, and manuscript preparation. The Subcontractor shall work closely with Dr. Mayali and other LLNL scientists, and shall participate in monthly SFA meetings (either in person or by telephone). The Subcontractor shall deliver a final report at the conclusion of the work.
ERIC Educational Resources Information Center
Lee, Connie W.; Hinson, Tony M.
This publication is the final report of a 21-month project designed to (1) expand and refine the computer capabilities of the Vocational-Technical Education Consortium of States (V-TECS) to ensure rapid data access for generating routine and special occupational data-based reports; (2) develop and implement a computer storage and retrieval system…
'Advancement of KHPS to DOE TRL 7/8' Project - Final Technical Report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Adonizio, Mary Ann; Corren, Dean; Smith, Ron
Final Report describing activities performed under the 'Advancement of the KHPS to DOE TRL 7/8' project, including the development of critical component test protocols, testing and analysis of the Gen5 KHPS main shaft seal, and continuing compliance work on approved operational environmental monitoring plans in anticipation of KHPS turbine installation at Verdant Power's Roosevelt Island Tidal Energy (RITE) Project site in New York, NY.
OPERATION CASTLE. Radiological Safety. Volume 1
1985-09-01
OPERATION CASTLE Radiological Safety Final Report Volume I Headquarters Joint Task Force Seven Technical Branch, J-3 Division Washington, DC...Spring 1954 EXTRACTED VERSION DTIC -uECTE MAR031986 NOTICE: This is an extract of Operation CASTLE, Radiological Safety, Final Report, Volume I ...SYMBOL (If jpQiictbl») ■ i PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER 8c AOORESS (G(y, SU(t tncl ZIRCod») 10 SOURCE OF FUNDING NUMBERS PROGRAM
European Upper Atmosphere Server DIAS - Final Conference/ Abstract
2007-01-10
PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) INGV - Istituto Nazionale di Geofisica e Vulcanologia (Nat Instit Geophysics, Volcanology) Via di Vigna...organised by the Istituto Nazionale di Geofisica e Vulcanologia (INGV), Rome, Italy. D6.8 Report on the Final Conference - 3 - In general, the DIAS...the Istituto Nazionale di Geofisica e Vulcanologia in Rome focused on the general overview of scientific and technical tools adopted by the DIAS
ERIC Educational Resources Information Center
Mallik, Kalisankar; Shaver, Elaine M.
The final report of a 3 year project to improve the quality of life of mentally retarded, cerebral palsied, and epileptic persons in Region III is presented. The first section details the efforts of the project staff in providing competitive employment opportunities or sheltered employment for 40 severely disabled persons. Equipment modification…
ERIC Educational Resources Information Center
Zuckerman, Arline; And Others
Six general recommendations reflect the findings from this final report on subject analysis of African and Asian library materials: (1) more specificity of headings, especially subdivisions and unique terms, are needed; (2) more attention should be given to Asian and African materials, using available expertise; (3) efforts to eliminate offensive…
DOE Office of Scientific and Technical Information (OSTI.GOV)
Fowler, Robert
This is the final report for the UNC component of the SciDAD Institute for Sustained Performance, Energy, and Resilience. In this report, we describe activities on the SUPER project at RENCI at the University of North Carolina at Chapel Hill. While we focus particularly on UNC, we touch on project-wide activities as well as, on interactions with, and impacts on, other projects.
Alloy undercooling experiments
NASA Technical Reports Server (NTRS)
Flemings, Merton C.; Matson, Douglas M.
1995-01-01
The research accomplished during 1995 can be organized into three parts. The first task involves analyzing the results of microgravity experiments carried out using TEMPUS hardware during the IML-2 mission on STS-65. The second part was to finalize ground-based experimentation which supported the above flight sample analysis. The final part was to provide technical support for post-flight mission activities specifically aimed at improving TEMPUS performance for potential future missions.
The Family and Medical Leave Act. Final rule.
2013-02-06
This Final Rule amends certain regulations of the Family and Medical Leave Act of 1993 (the FMLA or the Act) to implement amendments to the military leave provisions of the Act made by the National Defense Authorization Act for Fiscal Year 2010, which extends the availability of FMLA leave to family members of members of the Regular Armed Forces for qualifying exigencies arising out of the servicemember's deployment; defines those deployments covered under these provisions; extends FMLA military caregiver leave for family members of current servicemembers to include an injury or illness that existed prior to service and was aggravated in the line of duty on active duty; and extends FMLA military caregiver leave to family members of certain veterans with serious injuries or illnesses. This Final Rule also amends the regulations to implement the Airline Flight Crew Technical Corrections Act, which establishes eligibility requirements specifically for airline flight crewmembers and flight attendants for FMLA leave and authorizes the Department to issue regulations regarding the calculation of leave for such employees as well as special recordkeeping requirements for their employers. In addition, the Final Rule includes clarifying changes concerning the calculation of intermittent or reduced schedule FMLA leave; reorganization of certain sections to enhance clarity; the removal of the forms from the regulations; and technical corrections to the current regulations.
2012-11-15
This final rule with comment period revises the Medicare hospital outpatient prospective payment system (OPPS) and the Medicare ambulatory surgical center (ASC) payment system for CY 2013 to implement applicable statutory requirements and changes arising from our continuing experience with these systems. In this final rule with comment period, we describe the changes to the amounts and factors used to determine the payment rates for Medicare services paid under the OPPS and those paid under the ASC payment system. In addition, this final rule with comment period updates and refines the requirements for the Hospital Outpatient Quality Reporting (OQR) Program, the ASC Quality Reporting (ASCQR) Program, and the Inpatient Rehabilitation Facility (IRF) Quality Reporting Program. We are continuing the electronic reporting pilot for the Electronic Health Record (EHR) Incentive Program, and revising the various regulations governing Quality Improvement Organizations (QIOs), including the secure transmittal of electronic medical information, beneficiary complaint resolution and notification processes, and technical changes. The technical changes to the QIO regulations reflect CMS' commitment to the general principles of the President's Executive Order on Regulatory Reform, Executive Order 13563 (January 18, 2011).
A Control Strategy for High-Performance Macromolecular Materials
2007-01-04
None as of this date. Potential transitions with Moldflow Corporation, Boston, MA. New Discoveries None as of this date. Contract FA9550-06-C-0017, Final Technical Report, Submitted by Nonlinear Control Strategies, Inc.
Mariano Lake Mine: Technical Reports
This Final Removal Site Evaluation describes the objectives, work performed, and results of a Removal Site Evaluation (RSE) at the Mariano Lake Mine, which was performed by ARCADIS U.S., Inc. on behalf of Chevron Environmental Management Company.
Utah DOT weather responsive traveler information system.
DOT National Transportation Integrated Search
1996-06-01
The Final Performance and Benefits Summary describes our understanding of the connection between the National ITS Architecture, its technical performance characteristics, and its likely benefits for ITS users and suppliers. Ultimately, the goal of th...
Transit Reliability Information Program (TRIP) : Final Technical Report
DOT National Transportation Integrated Search
1984-05-01
The Transit Reliability Information Program (TRIP) is a government-initiated program to assist the transit industry in satisfying its need for rail transit car subsystem reliability information. TRIP provided this assistance through the operation of ...
Beams from boltwood: a feasibility study
Peter Koch
1964-01-01
Previous papers in this series of four have explored technical aspects of converting southern pine boltwood into long laminated beams of uniform high strength. This final discussion examines production and economic aspects of the proposed system.
Bringing Advanced Computational Techniques to Energy Research
DOE Office of Scientific and Technical Information (OSTI.GOV)
Mitchell, Julie C
2012-11-17
Please find attached our final technical report for the BACTER Institute award. BACTER was created as a graduate and postdoctoral training program for the advancement of computational biology applied to questions of relevance to bioenergy research.
NASA Technical Reports Server (NTRS)
Davis, M. H.
1981-01-01
Final development of a gravimetric test for performance evaluation of a precision saturator is described. The design and development of a prototype droplet levitation chamber is discussed. Technical assistance to the MSFC Airborne Laser Doppler Program is reported.
Reliability of Visual Inspection for Highway Bridges, Volume I : Final Report
DOT National Transportation Integrated Search
2001-06-01
This technical summary announces the findings of an investigation by the Federal Highway Administrations Nondestructive Evaluation Validation Center (NDEVC) concerning the reliability of Visual Inspection for highway bridges. Details and results a...