Science.gov

Sample records for active hyperspectral imaging

  1. Hyperspectral image segmentation using active contours

    NASA Astrophysics Data System (ADS)

    Lee, Cheolha P.; Snyder, Wesley E.

    2004-08-01

    Multispectral or hyperspectral image processing has been studied as a possible approach to automatic target recognition (ATR). Hundreds of spectral bands may provide high data redundancy, compensating the low contrast in medium wavelength infrared (MWIR) and long wavelength infrared (LWIR) images. Thus, the combination of spectral (image intensity) and spatial (geometric feature) information analysis could produce a substantial improvement. Active contours provide segments with continuous boundaries, while edge detectors based on local filtering often provide discontinuous boundaries. The segmentation by active contours depends on geometric feature of the object as well as image intensity. However, the application of active contours to multispectral images has been limited to the cases of simply textured images with low number of frames. This paper presents a supervised active contour model, which is applicable to vector-valued images with non-homogeneous regions and high number of frames. In the training stage, histogram models of target classes are estimated from sample vector-pixels. In the test stage, contours are evolved based on two different metrics: the histogram models of the corresponding segments and the histogram models estimated from sample target vector-pixels. The proposed segmentation method integrates segmentation and model-based pattern matching using supervised segmentation and multi-phase active contour model, while traditional methods apply pattern matching only after the segmentation. The proposed algorithm is implemented with both synthetic and real multispectral images, and shows desirable segmentation and classification results even in images with non-homogeneous regions.

  2. Active Volcano Monitoring using a Space-based Hyperspectral Imager

    NASA Astrophysics Data System (ADS)

    Cipar, J. J.; Dunn, R.; Cooley, T.

    2010-12-01

    Active volcanoes occur on every continent, often in close proximity to heavily populated areas. While ground-based studies are essential for scientific research and disaster mitigation, remote sensing from space can provide rapid and continuous monitoring of active and potentially active volcanoes [Ramsey and Flynn, 2004]. In this paper, we report on hyperspectral measurements of Kilauea volcano, Hawaii. Hyperspectral images obtained by the US Air Force TacSat-3/ARTEMIS sensor [Lockwood et al, 2006] are used to obtain estimates of the surface temperatures for the volcano. ARTEMIS measures surface-reflected light in the visible, near-infrared, and short-wave infrared bands (VNIR-SWIR). The SWIR bands are known to be sensitive to thermal radiation [Green, 1996]. For example, images from the NASA Hyperion hyperspectral sensor have shown the extent of wildfires and active volcanoes [Young, 2009]. We employ the methodology described by Dennison et al, (2006) to obtain an estimate of the temperature of the active region of Kilauea. Both day and night-time images were used in the analysis. To improve the estimate, we aggregated neighboring pixels. The active rim of the lava lake is clearly discernable in the temperature image, with a measured temperature exceeding 1100o C. The temperature decreases markedly on the exterior of the summit crater. While a long-wave infrared (LWIR) sensor would be ideal for volcano monitoring, we have shown that the thermal state of an active volcano can be monitored using the SWIR channels of a reflective hyperspectral imager. References: Dennison, Philip E., Kraivut Charoensiri, Dar A. Roberts, Seth H. Peterson, and Robert O. Green (2006). Wildfire temperature and land cover modeling using hyperspectral data, Remote Sens. Environ., vol. 100, pp. 212-222. Green, R. O. (1996). Estimation of biomass fire temperature and areal extent from calibrated AVIRIS spectra, in Summaries of the 6th Annual JPL Airborne Earth Science Workshop, Pasadena, CA

  3. Hyperspectral image processing

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral image processing refers to the use of computer algorithms to extract, store and manipulate both spatial and spectral information contained in hyperspectral images across the visible and near-infrared portion of the electromagnetic spectrum. A typical hyperspectral image processing work...

  4. Hyperspectral image processing methods

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral image processing refers to the use of computer algorithms to extract, store and manipulate both spatial and spectral information contained in hyperspectral images across the visible and near-infrared portion of the electromagnetic spectrum. A typical hyperspectral image processing work...

  5. Range-gated intensified spectrographic imager: an instrument for active hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Simard, Jean-Robert; Mathieu, Pierre; Fournier, Georges R.; Larochelle, Vincent; Babey, Stephen K.

    2000-09-01

    Hyperspectral imaging has demonstrated impressive capabilities in airborne surveys, particularly for mineral and biomass characterizations. Based on this success, it is believed that other applications like search and rescue operations, and detection/identification of various ground military targets could greatly benefit from this technology. The strength of hyperspectral imaging comes from the access to another dimension of information: the spectral content of the detected return signal for each spatial pixel. In the case of conventional hyperspectral imaging, the return signal depicts the spectral reflectance of the day irradiance from the scene within the field of view of each pixel. However, by inserting a range-gated intensifier into a hyperspectral camera and by combining the camera with selected pulsed lasers, it becomes possible to relate the returned spectral information to specific light/matter interactions like induced fluorescence. This new technique may be referred to as 'active hyperspectral imaging.' Among its advantages, this approach is independent of the ambient lighting conditions and can be customized in excitation wavelengths. Moreover, by using a range-gated intensified camera, it is possible to survey limited area with a significant increase in signal-to-noise ratio. A camera of this type has been built by our group in collaboration with private industry and is described in this paper. The internal design of the camera is discussed, new issues concerning the calibration of the camera are depicted and a model based on signal-to-noise ratio analysis is presented. From the fluorescent characteristics of surrogate land mines measured in the laboratory, this model is used to predict the capabilities of detecting surface-laid mines from an aerial platform based scenario.

  6. Multipurpose hyperspectral imaging system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral imaging system of high spectral and spatial resolution that incorporates several innovative features has been developed to incorporate a focal plane scanner (U.S. Patent 6,166,373). This feature enables the system to be used for both airborne/spaceborne and laboratory hyperspectral i...

  7. Person detection in hyperspectral images via skin segmentation using an active learning approach

    NASA Astrophysics Data System (ADS)

    Marqués, Ion; Graña, Manuel; Sanchez, Stephanie M.; Alkhatib, Mohammed Q.; Velez-Reyes, Miguel

    2015-05-01

    Human skin detection is a computer vision problem that has been widely researched in color images. In this article we deal with this task as an interactive segmentation problem in hyperspectral outdoor images. We have focused on the problem of skin identification in hyperspectral cameras allowing a fine sampling of the light spectrum, so that the information gathered at each pixel is a high dimensional vector. The problem is treated as a classification problem, where we make use of active learning strategies to provide an interactive robust solution reaching high accuracy in a short training/testing cycle.

  8. Handheld hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Saari, Heikki; Aallos, Ville-Veikko; Holmlund, Christer; Malinen, Jouko; Mäkynen, Jussi

    2010-04-01

    VTT Technical Research Centre of Finland has developed a new low cost hand-held staring hyperspectral imager for applications previously blocked by high cost of the instrumentation. The system is compatible with standard video and microscope lenses. The instrument can record 2D spatial images at several wavelength bands simultaneously. The concept of the hyperspectral imager has been published in SPIE Proc. 7474. The prototype fits in an envelope of 100 mm x 60 mm x 40 mm and its weight is ca. 300 g. The benefits of the new device compared to Acousto-Optic Tunable filter (AOTF) or Liquid Crystal Tunable Filter (LCTF) devices are small size and weight, speed of wavelength tuning, high optical throughput, independence of polarization state of incoming light and capability to record three wavelengths simultaneously. The operational wavelength range with Silicon-based CCD or CMOS sensors is 200 - 1100 nm and spectral resolution is 2 - 10 nm @ FWHM. Similar IR imagers can be built using InGaAs, InSb or MCT imaging sensors. The spatial resolution of the prototype is 480 x 750 pixels. It contains control system and memory for the image data acquisition. It operates either autonomously recording hyperspectral data cubes continuously or controlled by a laptop computer. The prototype was configured as a hyperspectral microscope for the spectral range 400 - 700 nm. The design of the hyperspectral imager, characterization results and sample measurement results are presented.

  9. Miniaturized handheld hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Wu, Huawen; Haibach, Frederick G.; Bergles, Eric; Qian, Jack; Zhang, Charlie; Yang, William

    2014-05-01

    A miniaturized hyperspectral imager is enabled with image sensor integrated with dispersing elements in a very compact form factor, removing the need for expensive, moving, bulky and complex optics that have been used in conventional hyperspectral imagers for decades. The result is a handheld spectral imager that can be installed on miniature UAV drones or conveyor belts in production lines. Eventually, small handhelds can be adapted for use in outpatient medical clinics for point-of-care diagnostics and other in-field applications.

  10. Hyperspectral light field imaging

    NASA Astrophysics Data System (ADS)

    Leitner, Raimund; Kenda, Andreas; Tortschanoff, Andreas

    2015-05-01

    A light field camera acquires the intensity and direction of rays from a scene providing a 4D representation L(x,y,u,v) called the light field. The acquired light field allows to virtually change view point and selectively re-focus regions algorithmically, an important feature for many applications in imaging and microscopy. The combination with hyperspectral imaging provides the additional advantage that small objects (beads, cells, nuclei) can be categorised using their spectroscopic signatures. Using an inverse fluorescence microscope, a LCTF tuneable filter and a light field setup as a test-bed, fluorescence-marked beads have been imaged and reconstructed into a 4D hyper-spectral image cube LHSI(x,y,z,λ). The results demonstrate the advantages of the approach for fluorescence microscopy providing extended depth of focus (DoF) and the fidelity of hyper-spectral imaging.

  11. Unsupervised hyperspectral image classification

    NASA Astrophysics Data System (ADS)

    Jiao, Xiaoli; Chang, Chein-I.

    2007-09-01

    Two major issues encountered in unsupervised hyperspectral image classification are (1) how to determine the number of spectral classes in the image and (2) how to find training samples that well represent each of spectral classes without prior knowledge. A recently developed concept, Virtual dimensionality (VD) is used to estimate the number of spectral classes of interest in the image data. This paper proposes an effective algorithm to generate an appropriate training set via a recently developed Prioritized Independent Component Analysis (PICA). Two sets of hyperspectral data, Airborne Visible Infrared Imaging Spectrometer (AVIRIS) Cuprite data and HYperspectral Digital Image Collection Experiment (HYDICE) data are used for experiments and performance analysis for the proposed method.

  12. Hyperspectral fundus imager

    NASA Astrophysics Data System (ADS)

    Truitt, Paul W.; Soliz, Peter; Meigs, Andrew D.; Otten, Leonard John, III

    2000-11-01

    A Fourier Transform hyperspectral imager was integrated onto a standard clinical fundus camera, a Zeiss FF3, for the purposes of spectrally characterizing normal anatomical and pathological features in the human ocular fundus. To develop this instrument an existing FDA approved retinal camera was selected to avoid the difficulties of obtaining new FDA approval. Because of this, several unusual design constraints were imposed on the optical configuration. Techniques to calibrate the sensor and to define where the hyperspectral pushbroom stripe was located on the retina were developed, including the manufacturing of an artificial eye with calibration features suitable for a spectral imager. In this implementation the Fourier transform hyperspectral imager can collect over a hundred 86 cm-1 spectrally resolved bands with 12 micro meter/pixel spatial resolution within the 1050 nm to 450 nm band. This equates to 2 nm to 8 nm spectral resolution depending on the wavelength. For retinal observations the band of interest tends to lie between 475 nm and 790 nm. The instrument has been in use over the last year successfully collecting hyperspectral images of the optic disc, retinal vessels, choroidal vessels, retinal backgrounds, and macula diabetic macular edema, and lesions of age-related macular degeneration.

  13. Quantitative Hyperspectral Reflectance Imaging

    PubMed Central

    Klein, Marvin E.; Aalderink, Bernard J.; Padoan, Roberto; de Bruin, Gerrit; Steemers, Ted A.G.

    2008-01-01

    Hyperspectral imaging is a non-destructive optical analysis technique that can for instance be used to obtain information from cultural heritage objects unavailable with conventional colour or multi-spectral photography. This technique can be used to distinguish and recognize materials, to enhance the visibility of faint or obscured features, to detect signs of degradation and study the effect of environmental conditions on the object. We describe the basic concept, working principles, construction and performance of a laboratory instrument specifically developed for the analysis of historical documents. The instrument measures calibrated spectral reflectance images at 70 wavelengths ranging from 365 to 1100 nm (near-ultraviolet, visible and near-infrared). By using a wavelength tunable narrow-bandwidth light-source, the light energy used to illuminate the measured object is minimal, so that any light-induced degradation can be excluded. Basic analysis of the hyperspectral data includes a qualitative comparison of the spectral images and the extraction of quantitative data such as mean spectral reflectance curves and statistical information from user-defined regions-of-interest. More sophisticated mathematical feature extraction and classification techniques can be used to map areas on the document, where different types of ink had been applied or where one ink shows various degrees of degradation. The developed quantitative hyperspectral imager is currently in use by the Nationaal Archief (National Archives of The Netherlands) to study degradation effects of artificial samples and original documents, exposed in their permanent exhibition area or stored in their deposit rooms.

  14. Multipurpose Hyperspectral Imaging System

    NASA Technical Reports Server (NTRS)

    Mao, Chengye; Smith, David; Lanoue, Mark A.; Poole, Gavin H.; Heitschmidt, Jerry; Martinez, Luis; Windham, William A.; Lawrence, Kurt C.; Park, Bosoon

    2005-01-01

    A hyperspectral imaging system of high spectral and spatial resolution that incorporates several innovative features has been developed to incorporate a focal plane scanner (U.S. Patent 6,166,373). This feature enables the system to be used for both airborne/spaceborne and laboratory hyperspectral imaging with or without relative movement of the imaging system, and it can be used to scan a target of any size as long as the target can be imaged at the focal plane; for example, automated inspection of food items and identification of single-celled organisms. The spectral resolution of this system is greater than that of prior terrestrial multispectral imaging systems. Moreover, unlike prior high-spectral resolution airborne and spaceborne hyperspectral imaging systems, this system does not rely on relative movement of the target and the imaging system to sweep an imaging line across a scene. This compact system (see figure) consists of a front objective mounted at a translation stage with a motorized actuator, and a line-slit imaging spectrograph mounted within a rotary assembly with a rear adaptor to a charged-coupled-device (CCD) camera. Push-broom scanning is carried out by the motorized actuator which can be controlled either manually by an operator or automatically by a computer to drive the line-slit across an image at a focal plane of the front objective. To reduce the cost, the system has been designed to integrate as many as possible off-the-shelf components including the CCD camera and spectrograph. The system has achieved high spectral and spatial resolutions by using a high-quality CCD camera, spectrograph, and front objective lens. Fixtures for attachment of the system to a microscope (U.S. Patent 6,495,818 B1) make it possible to acquire multispectral images of single cells and other microscopic objects.

  15. Active infrared hyperspectral imaging system using a broadly tunable optical parametric oscillator

    NASA Astrophysics Data System (ADS)

    Malcolm, G. P. A.; Maker, G. T.; Robertson, G.; Dunn, M. H.; Stothard, D. J. M.

    2009-09-01

    The in situ identification and spatial location of gases, discrete liquid droplets and residues on surfaces is a technically challenging problem. Active Infrared (IR) hyperspectral imaging is a powerful technique that combines real-time imaging and optical spectroscopy for "standoff" detection of suspected chemical substances, including chemical warfare agents, toxic industrial chemicals, explosives and narcotics. An active IR hyperspectral imaging system requires a coherent, broadly tunable IR light source of high spectral purity, in order to detect a broad range of target substances. In this paper we outline a compact and power-efficient IR illumination source with high stability, efficiency, tuning range and spectral purity based upon an optical parametric oscillator (OPO). The fusion of established OPO technology with novel diode-pumped laser technology and electro-mechanical scanning has enabled a broadly applicable imaging system. This system is capable of hyperspectral imaging at both Near-IR (1.3 - 1.9 μm) and Mid-IR (2.3 - 4.6 μm) wavelengths simultaneously with a line width of < 3 cm-1. System size and complexity are minimised by using a dual InGaAs/InSb single element detector, and images are acquired by raster scanning the coaxial signal and idler beams simultaneously, at ranges up to 20 m. Reflection, absorption and scatter of incident radiation by chemical targets and their surroundings provide a method for spatial location, and characteristic spectra obtained from each sample can be used to identify targets uniquely. To date, we have recognized liquids in sample sizes as small 20 μl-and gases with sensitivity as high as 10ppm.m-at detection standoff distances > 10 m.

  16. Planetary Hyperspectral Imager (PHI)

    NASA Technical Reports Server (NTRS)

    Silvergate, Peter

    1996-01-01

    A hyperspectral imaging spectrometer was breadboarded. Key innovations were use of a sapphire prism and single InSb focal plane to cover the entire spectral range, and a novel slit optic and relay optics to reduce thermal background. Operation over a spectral range of 450 - 4950 nm (approximately 3.5 spectral octaves) was demonstrated. Thermal background reduction by a factor of 8 - 10 was also demonstrated.

  17. Simulation of Hyperspectral Images

    NASA Technical Reports Server (NTRS)

    Richsmeier, Steven C.; Singer-Berk, Alexander; Bernstein, Lawrence S.

    2004-01-01

    A software package generates simulated hyperspectral imagery for use in validating algorithms that generate estimates of Earth-surface spectral reflectance from hyperspectral images acquired by airborne and spaceborne instruments. This software is based on a direct simulation Monte Carlo approach for modeling three-dimensional atmospheric radiative transport, as well as reflections from surfaces characterized by spatially inhomogeneous bidirectional reflectance distribution functions. In this approach, "ground truth" is accurately known through input specification of surface and atmospheric properties, and it is practical to consider wide variations of these properties. The software can treat both land and ocean surfaces, as well as the effects of finite clouds with surface shadowing. The spectral/spatial data cubes computed by use of this software can serve both as a substitute for, and a supplement to, field validation data.

  18. Airborne Hyperspectral Imaging System

    NASA Technical Reports Server (NTRS)

    Behar, Alberto E.; Cooper, Moogega; Adler, John; Jacobson, Tobias

    2012-01-01

    A document discusses a hyperspectral imaging instrument package designed to be carried aboard a helicopter. It was developed to map the depths of Greenland's supraglacial lakes. The instrument is capable of telescoping to twice its original length, allowing it to be retracted with the door closed during takeoff and landing, and manually extended in mid-flight. While extended, the instrument platform provides the attached hyperspectral imager a nadir-centered and unobstructed view of the ground. Before flight, the instrument mount is retracted and securely strapped down to existing anchor points on the floor of the helicopter. When the helicopter reaches the destination lake, the door is opened and the instrument mount is manually extended. Power to the instrument package is turned on, and the data acquisition computer is commanded via a serial cable from an onboard user-operated laptop to begin data collection. After data collection is complete, the instrument package is powered down and the mount retracted, allowing the door to be closed in preparation for landing. The present design for the instrument mount consists of a three-segment telescoping cantilever to allow for a sufficient extended length to see around the landing struts and provide a nadir-centered and unobstructed field of view for the hyperspectral imager. This instrument works on the premise that water preferentially absorbs light with longer wavelengths on the red side of the visible spectrum. This property can be exploited in order to remotely determine the depths of bodies of pure freshwater. An imager flying over such a lake receives light scattered from the surface, the bulk of the water column, and from the lake bottom. The strength of absorption of longer-wavelength light depends on the depth of the water column. Through calibration with in situ measurements of the water depths, a depth-determining algorithm may be developed to determine lake depth from these spectral properties of the

  19. Hyperspectral Imager-Tracker

    NASA Technical Reports Server (NTRS)

    Agurok, Llya

    2013-01-01

    The Hyperspectral Imager-Tracker (HIT) is a technique for visualization and tracking of low-contrast, fast-moving objects. The HIT architecture is based on an innovative and only recently developed concept in imaging optics. This innovative architecture will give the Light Prescriptions Innovators (LPI) HIT the possibility of simultaneously collecting the spectral band images (hyperspectral cube), IR images, and to operate with high-light-gathering power and high magnification for multiple fast- moving objects. Adaptive Spectral Filtering algorithms will efficiently increase the contrast of low-contrast scenes. The most hazardous parts of a space mission are the first stage of a launch and the last 10 kilometers of the landing trajectory. In general, a close watch on spacecraft operation is required at distances up to 70 km. Tracking at such distances is usually associated with the use of radar, but its milliradian angular resolution translates to 100- m spatial resolution at 70-km distance. With sufficient power, radar can track a spacecraft as a whole object, but will not provide detail in the case of an accident, particularly for small debris in the onemeter range, which can only be achieved optically. It will be important to track the debris, which could disintegrate further into more debris, all the way to the ground. Such fragmentation could cause ballistic predictions, based on observations using high-resolution but narrow-field optics for only the first few seconds of the event, to be inaccurate. No optical imager architecture exists to satisfy NASA requirements. The HIT was developed for space vehicle tracking, in-flight inspection, and in the case of an accident, a detailed recording of the event. The system is a combination of five subsystems: (1) a roving fovea telescope with a wide 30 field of regard; (2) narrow, high-resolution fovea field optics; (3) a Coude optics system for telescope output beam stabilization; (4) a hyperspectral

  20. Hyperspectral image analysis. A tutorial.

    PubMed

    Amigo, José Manuel; Babamoradi, Hamid; Elcoroaristizabal, Saioa

    2015-10-01

    This tutorial aims at providing guidelines and practical tools to assist with the analysis of hyperspectral images. Topics like hyperspectral image acquisition, image pre-processing, multivariate exploratory analysis, hyperspectral image resolution, classification and final digital image processing will be exposed, and some guidelines given and discussed. Due to the broad character of current applications and the vast number of multivariate methods available, this paper has focused on an industrial chemical framework to explain, in a step-wise manner, how to develop a classification methodology to differentiate between several types of plastics by using Near infrared hyperspectral imaging and Partial Least Squares - Discriminant Analysis. Thus, the reader is guided through every single step and oriented in order to adapt those strategies to the user's case. PMID:26481986

  1. Hyperspectral Systems Increase Imaging Capabilities

    NASA Technical Reports Server (NTRS)

    2010-01-01

    In 1983, NASA started developing hyperspectral systems to image in the ultraviolet and infrared wavelengths. In 2001, the first on-orbit hyperspectral imager, Hyperion, was launched aboard the Earth Observing-1 spacecraft. Based on the hyperspectral imaging sensors used in Earth observation satellites, Stennis Space Center engineers and Institute for Technology Development researchers collaborated on a new design that was smaller and used an improved scanner. Featured in Spinoff 2007, the technology is now exclusively licensed by Themis Vision Systems LLC, of Richmond, Virginia, and is widely used in medical and life sciences, defense and security, forensics, and microscopy.

  2. Medical hyperspectral imaging: a review

    NASA Astrophysics Data System (ADS)

    Lu, Guolan; Fei, Baowei

    2014-01-01

    Hyperspectral imaging (HSI) is an emerging imaging modality for medical applications, especially in disease diagnosis and image-guided surgery. HSI acquires a three-dimensional dataset called hypercube, with two spatial dimensions and one spectral dimension. Spatially resolved spectral imaging obtained by HSI provides diagnostic information about the tissue physiology, morphology, and composition. This review paper presents an overview of the literature on medical hyperspectral imaging technology and its applications. The aim of the survey is threefold: an introduction for those new to the field, an overview for those working in the field, and a reference for those searching for literature on a specific application.

  3. Medical hyperspectral imaging: a review

    PubMed Central

    Lu, Guolan; Fei, Baowei

    2014-01-01

    Abstract. Hyperspectral imaging (HSI) is an emerging imaging modality for medical applications, especially in disease diagnosis and image-guided surgery. HSI acquires a three-dimensional dataset called hypercube, with two spatial dimensions and one spectral dimension. Spatially resolved spectral imaging obtained by HSI provides diagnostic information about the tissue physiology, morphology, and composition. This review paper presents an overview of the literature on medical hyperspectral imaging technology and its applications. The aim of the survey is threefold: an introduction for those new to the field, an overview for those working in the field, and a reference for those searching for literature on a specific application. PMID:24441941

  4. Satellite Hyperspectral Imaging Simulation

    NASA Technical Reports Server (NTRS)

    Zanoni, Vicki; Stanley, Tom; Blonski, Slawomir; Cao, Changyong; Gasser, Jerry; Ryan, Robert

    1999-01-01

    Simulation of generic pushbroom satellite hyperspectral sensors have been performed to evaluate the potential performance and validation techniques for satellite systems such as COIS(NEMO), Warfighter-1(OrbView-4) and Hyperion(EO-1). The simulations start with a generation of synthetic scenes from material maps of studied terrain. Scene-reflected radiance is corrected for atmospheric effects and convolved with sensor spectral response using MODTRAN 4 radiance and transmissions calculations. Scene images are further convolved with point spread functions derived from Optical Transfer Functions (OTF's) of the sensor system. Photon noise and etectorr/electronics noise are added to the simulated images, which are also finally quantized to the sensor bit resolution. Studied scenes include bridges and straight roads used for evaluation of sensor spatial resolution, as well as fields of minerals, vegetation and manmade materials used for evaluation of sensor radiometric response and sensitivity. The scenes are simulated with various seasons and weather conditions. Signal-to-noise ratios and expected performance are estimated for typical satellite system specifications and are discussed for all the scenes.

  5. Fiber optic snapshot hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Mansur, David J.; Rentz Dupuis, Julia; Vaillancourt, Robert

    2012-06-01

    OPTRA is developing a snapshot hyperspectral imager (HSI) employing a fiber optic bundle and dispersive spectrometer. The fiber optic bundle converts a broadband spatial image to an array of fiber columns which serve as multiple entrance slits to a prism spectrometer. The dispersed spatially resolved spectra are then sampled by a two-dimensional focal plane array (FPA) at a greater than 30 Hz update rate, thereby qualifying the system as snapshot. Unlike snapshot HSI systems based on computed tomography or coded apertures, our approach requires only the remapping of the FPA frame into hyperspectral cubes rather than a complex reconstruction. Our system has high radiometric efficiency and throughput supporting sufficient signal to noise for hyperspectral imaging measurements made over very short integration times (< 33 ms). The overall approach is compact, low cost, and contains no moving parts, making it ideal for unmanned airborne surveillance. In this paper we present a preliminary design for the fiber optic snapshot HSI system.

  6. Bayesian segmentation of hyperspectral images

    NASA Astrophysics Data System (ADS)

    Mohammadpour, Adel; Féron, Olivier; Mohammad-Djafari, Ali

    2004-11-01

    In this paper we consider the problem of joint segmentation of hyperspectral images in the Bayesian framework. The proposed approach is based on a Hidden Markov Modeling (HMM) of the images with common segmentation, or equivalently with common hidden classification label variables which is modeled by a Potts Markov Random Field. We introduce an appropriate Markov Chain Monte Carlo (MCMC) algorithm to implement the method and show some simulation results.

  7. Hyperspectral imaging system for UAV

    NASA Astrophysics Data System (ADS)

    Zhang, Da; Zheng, Yuquan

    2015-10-01

    Hyperspectral imaging system for Unmanned Aerial Vehicle (UAV) is proposed under airborne remote sensing application background. By the application of Offner convex spherical grating spectral imaging system and using large area array detector push-broom imaging, hyperspectral imaging system with the indicators of 0.4μm to 1.0μm spectral range, 120 spectral bands, 5nm spectral resolution and 1m ground sampling interval (flight altitude 5km) is developed and completed. The Offner convex grating spectral imaging system is selected to achieve non-spectral line bending and colorless distortion design results. The diffraction efficiency is 15%-30% in the range of 0.4μm to 1.0μm wavelength. The system performances are tested by taking spectral and radiometric calibration methods in the laboratory. Based on monochromatic collimated light method for spectral performance parameters calibration of hyperspectral optical remote sensor, the analysis results of spectral calibration data show that the calibration test repeatability is less than 0.2 nm within one hour. The spectral scaling results show that the average spectral resolution of hyperspectral optical remote sensor is 4.94 nm, and the spatial dimension of the high-spectral optical remote sensor spectral resolution is less than 5 nm, the average of the typical spectral bandwidth is about 6 nm, the system average signal-to-noise ratio (SNR) is up to 43dB under typical operating conditions. Finally the system functionalities and performance indicators are verified by the aviation flight tests, which it's equipped on UAV. The actual image quality is good, and the spectral position is stable.

  8. Hyperspectral imaging of bruised skin

    NASA Astrophysics Data System (ADS)

    Randeberg, Lise L.; Baarstad, Ivar; Løke, Trond; Kaspersen, Peter; Svaasand, Lars O.

    2006-02-01

    Bruises can be important evidence in legal medicine, for example in cases of child abuse. Optical techniques can be used to discriminate and quantify the chromophores present in bruised skin, and thereby aid dating of an injury. However, spectroscopic techniques provide only average chromophore concentrations for the sampled volume, and contain little information about the spatial chromophore distribution in the bruise. Hyperspectral imaging combines the power of imaging and spectroscopy, and can provide both spectroscopic and spatial information. In this study a hyperspectral imaging system developed by Norsk Elektro Optikk AS was used to measure the temporal development of bruised skin in a human volunteer. The bruises were inflicted by paintball bullets. The wavelength ranges used were 400 - 1000 nm (VNIR) and 900 - 1700 nm (SWIR), and the spectral sampling intervals were 3.7 and 5 nm, respectively. Preliminary results show good spatial discrimination of the bruised areas compared to normal skin. Development of a white spot can be seen in the central zone of the bruises. This central white zone was found to resemble the shape of the object hitting the skin, and is believed to develop in areas where the impact caused vessel damage. These results show that hyperspectral imaging is a promising technique to evaluate the temporal and spatial development of bruises on human skin.

  9. Longwave infrared compressive hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Dupuis, Julia R.; Kirby, Michael; Cosofret, Bogdan R.

    2015-06-01

    Physical Sciences Inc. (PSI) is developing a longwave infrared (LWIR) compressive sensing hyperspectral imager (CS HSI) based on a single pixel architecture for standoff vapor phase plume detection. The sensor employs novel use of a high throughput stationary interferometer and a digital micromirror device (DMD) converted for LWIR operation in place of the traditional cooled LWIR focal plane array. The CS HSI represents a substantial cost reduction over the state of the art in LWIR HSI instruments. Radiometric improvements for using the DMD in the LWIR spectral range have been identified and implemented. In addition, CS measurement and sparsity bases specifically tailored to the CS HSI instrument and chemical plume imaging have been developed and validated using LWIR hyperspectral image streams of chemical plumes. These bases enable comparable statistics to detection based on uncompressed data. In this paper, we present a system model predicting the overall performance of the CS HSI system. Results from a breadboard build and test validating the system model are reported. In addition, the measurement and sparsity basis work demonstrating the plume detection on compressed hyperspectral images is presented.

  10. A Group-Lasso Active Set Strategy for Multiclass Hyperspectral Image Classification

    NASA Astrophysics Data System (ADS)

    Tuia, D.; Courty, N.; Flamary, R.

    2014-08-01

    Hyperspectral images have a strong potential for landcover/landuse classification, since the spectra of the pixels can highlight subtle differences between materials and provide information beyond the visible spectrum. Yet, a limitation of most current approaches is the hypothesis of spatial independence between samples: images are spatially correlated and the classification map should exhibit spatial regularity. One way of integrating spatial smoothness is to augment the input spectral space with filtered versions of the bands. However, open questions remain, such as the selection of the bands to be filtered, or the filterbank to be used. In this paper, we consider the entirety of the possible spatial filters by using an incremental feature learning strategy that assesses whether a candidate feature would improve the model if added to the current input space. Our approach is based on a multiclass logistic classifier with group-lasso regularization. The optimization of this classifier yields an optimality condition, that can easily be used to assess the interest of a candidate feature without retraining the model, thus allowing drastic savings in computational time. We apply the proposed method to three challenging hyperspectral classification scenarios, including agricultural and urban data, and study both the ability of the incremental setting to learn features that always improve the model and the nature of the features selected.

  11. Quality assessment for hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Chen, Yuheng; Chen, Xinhua; Zhou, Jiankang; Shen, Weimin

    2014-11-01

    Image quality assessment is an essential value judgement approach for many applications. Multi & hyper spectral imaging has more judging essentials than grey scale or RGB imaging and its image quality assessment job has to cover up all-around evaluating factors. This paper presents an integrating spectral imaging quality assessment project, in which spectral-based, radiometric-based and spatial-based statistical behavior for three hyperspectral imagers are jointly executed. Spectral response function is worked out based on discrete illumination images and its spectral performance is deduced according to its FWHM and spectral excursion value. Radiometric response ability of different spectral channel under both on-ground and airborne imaging condition is judged by SNR computing based upon local RMS extraction and statistics method. Spatial response evaluation of the spectral imaging instrument is worked out by MTF computing with slanted edge analysis method. Reported pioneering systemic work in hyperspectral imaging quality assessment is carried out with the help of several domestic dominating work units, which not only has significance in the development of on-ground and in-orbit instrument performance evaluation technique but also takes on reference value for index demonstration and design optimization for instrument development.

  12. Compact hyperspectral image sensor based on a novel hyperspectral encoder

    NASA Astrophysics Data System (ADS)

    Hegyi, Alex N.; Martini, Joerg

    2015-06-01

    A novel hyperspectral imaging sensor is demonstrated that can enable breakthrough applications of hyperspectral imaging in domains not previously accessible. Our technology consists of a planar hyperspectral encoder combined with a traditional monochrome image sensor. The encoder adds negligibly to the sensor's overall size, weight, power requirement, and cost (SWaP-C); therefore, the new imager can be incorporated wherever image sensors are currently used, such as in cell phones and other consumer electronics. In analogy to Fourier spectroscopy, the technique maintains a high optical throughput because narrow-band spectral filters are unnecessary. Unlike conventional Fourier techniques that rely on Michelson interferometry, our hyperspectral encoder is robust to vibration and amenable to planar integration. The device can be viewed within a computational optics paradigm: the hardware is uncomplicated and serves to increase the information content of the acquired data, and the complexity of the system, that is, the decoding of the spectral information, is shifted to computation. Consequently, system tradeoffs, for example, between spectral resolution and imaging speed or spatial resolution, are selectable in software. Our prototype demonstration of the hyperspectral imager is based on a commercially-available silicon CCD. The prototype encoder was inserted within the camera's ~1 cu. in. housing. The prototype can image about 49 independent spectral bands distributed from 350 nm to 1250 nm, but the technology may be extendable over a wavelength range from ~300 nm to ~10 microns, with suitable choice of detector.

  13. Infrared hyperspectral imaging for chemical vapour detection

    NASA Astrophysics Data System (ADS)

    Ruxton, K.; Robertson, G.; Miller, W.; Malcolm, G. P. A.; Maker, G. T.; Howle, C. R.

    2012-10-01

    Active hyperspectral imaging is a valuable tool in a wide range of applications. One such area is the detection and identification of chemicals, especially toxic chemical warfare agents, through analysis of the resulting absorption spectrum. This work presents a selection of results from a prototype midwave infrared (MWIR) hyperspectral imaging instrument that has successfully been used for compound detection at a range of standoff distances. Active hyperspectral imaging utilises a broadly tunable laser source to illuminate the scene with light at a range of wavelengths. While there are a number of illumination methods, the chosen configuration illuminates the scene by raster scanning the laser beam using a pair of galvanometric mirrors. The resulting backscattered light from the scene is collected by the same mirrors and focussed onto a suitable single-point detector, where the image is constructed pixel by pixel. The imaging instrument that was developed in this work is based around an IR optical parametric oscillator (OPO) source with broad tunability, operating in the 2.6 to 3.7 μm (MWIR) and 1.5 to 1.8 μm (shortwave IR, SWIR) spectral regions. The MWIR beam was primarily used as it addressed the fundamental absorption features of the target compounds compared to the overtone and combination bands in the SWIR region, which can be less intense by more than an order of magnitude. We show that a prototype NCI instrument was able to locate hydrocarbon materials at distances up to 15 metres.

  14. Fast detection of peroxidase (POD) activity in tomato leaves which infected with Botrytis cinerea using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Kong, Wenwen; Liu, Fei; Zhang, Chu; Bao, Yidan; Yu, Jiajia; He, Yong

    2014-01-01

    Tomatoes are cultivated around the world and gray mold is one of its most prominent and destructive diseases. An early disease detection method can decrease losses caused by plant diseases and prevent the spread of diseases. The activity of peroxidase (POD) is very important indicator of disease stress for plants. The objective of this study is to examine the possibility of fast detection of POD activity in tomato leaves which infected with Botrytis cinerea using hyperspectral imaging data. Five pre-treatment methods were investigated. Genetic algorithm-partial least squares (GA-PLS) was applied to select optimal wavelengths. A new fast learning neural algorithm named extreme learning machine (ELM) was employed as multivariate analytical tool in this study. 21 optimal wavelengths were selected by GA-PLS and used as inputs of three calibration models. The optimal prediction result was achieved by ELM model with selected wavelengths, and the r and RMSEP in validation were 0.8647 and 465.9880 respectively. The results indicated that hyperspectral imaging could be considered as a valuable tool for POD activity prediction. The selected wavelengths could be potential resources for instrument development.

  15. Hyperspectral imaging for nondestructive evaluation of tomatoes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Machine vision methods for quality and defect evaluation of tomatoes have been studied for online sorting and robotic harvesting applications. We investigated the use of a hyperspectral imaging system for quality evaluation and defect detection for tomatoes. Hyperspectral reflectance images were a...

  16. Hyperspectral imaging using compressed sensing

    NASA Astrophysics Data System (ADS)

    Ramirez I., Gabriel Eduardo; Manian, Vidya B.

    2012-06-01

    Compressed sensing (CS) has attracted a lot of attention in recent years as a promising signal processing technique that exploits a signal's sparsity to reduce its size. It allows for simple compression that does not require a lot of additional computational power, and would allow physical implementation at the sensor using spatial light multiplexers using Texas Instruments (TI) digital micro-mirror device (DMD). The DMD can be used as a random measurement matrix, reflecting the image off the DMD is the equivalent of an inner product between the images individual pixels and the measurement matrix. CS however is asymmetrical, meaning that the signals recovery or reconstruction from the measurements does require a higher level of computation. This makes the prospect of working with the compressed version of the signal in implementations such as detection or classification much more efficient. If an initial analysis shows nothing of interest, the signal need not be reconstructed. Many hyper-spectral image applications are precisely focused on these areas, and would greatly benefit from a compression technique like CS that could help minimize the light sensor down to a single pixel, lowering costs associated with the cameras while reducing the large amounts of data generated by all the bands. The present paper will show an implementation of CS using a single pixel hyper-spectral sensor, and compare the reconstructed images to those obtained through the use of a regular sensor.

  17. Hyperspectral Imaging of human arm

    NASA Technical Reports Server (NTRS)

    2003-01-01

    ProVision Technologies, a NASA research partnership center at Sternis Space Center in Mississippi, has developed a new hyperspectral imaging (HSI) system that is much smaller than the original large units used aboard remote sensing aircraft and satellites. The new apparatus is about the size of a breadbox. Health-related applications of HSI include non-invasive analysis of human skin to characterize wounds and wound healing rates (especially important for space travelers who heal more slowly), determining if burns are first-, second-, or third degree (rather than painful punch biopsies). The work is sponsored under NASA's Space Product Development (SPD) program.

  18. Hyperspectral imaging camera using wavefront division interference.

    PubMed

    Bahalul, Eran; Bronfeld, Asaf; Epshtein, Shlomi; Saban, Yoram; Karsenty, Avi; Arieli, Yoel

    2016-03-01

    An approach for performing hyperspectral imaging is introduced. The hyperspectral imaging is based on Fourier transform spectroscopy, where the interference is performed by wavefront division interference rather than amplitude division interference. A variable phase delay between two parts of the wavefront emanating from each point of an object is created by a spatial light modulator (SLM) to obtain variable interference patterns. The SLM is placed in the exit pupil of an imaging system, thus enabling conversion of a general imaging optical system into an imaging hyperspectral optical system. The physical basis of the new approach is introduced, and an optical apparatus is built. PMID:26974085

  19. Common hyperspectral image database design

    NASA Astrophysics Data System (ADS)

    Tian, Lixun; Liao, Ningfang; Chai, Ali

    2009-11-01

    This paper is to introduce Common hyperspectral image database with a demand-oriented Database design method (CHIDB), which comprehensively set ground-based spectra, standardized hyperspectral cube, spectral analysis together to meet some applications. The paper presents an integrated approach to retrieving spectral and spatial patterns from remotely sensed imagery using state-of-the-art data mining and advanced database technologies, some data mining ideas and functions were associated into CHIDB to make it more suitable to serve in agriculture, geological and environmental areas. A broad range of data from multiple regions of the electromagnetic spectrum is supported, including ultraviolet, visible, near-infrared, thermal infrared, and fluorescence. CHIDB is based on dotnet framework and designed by MVC architecture including five main functional modules: Data importer/exporter, Image/spectrum Viewer, Data Processor, Parameter Extractor, and On-line Analyzer. The original data were all stored in SQL server2008 for efficient search, query and update, and some advance Spectral image data Processing technology are used such as Parallel processing in C#; Finally an application case is presented in agricultural disease detecting area.

  20. Vessel contrast enhancement in hyperspectral images

    NASA Astrophysics Data System (ADS)

    Bjorgan, Asgeir; Denstedt, Martin; Milanič, Matija; Paluchowski, Lukasz A.; Randeberg, Lise L.

    2015-03-01

    Imaging of vessel structures can be useful for investigation of endothelial function, angiogenesis and hyper-vascularization. This can be challenging for hyperspectral tissue imaging due to photon scattering and absorption in other parts of the tissue. Real-time processing techniques for enhancement of vessel contrast in hyperspectral tissue images were investigated. Wavelet processing and an inverse diffusion model were employed, and compared to band ratio metrics and statistical methods. A multiscale vesselness filter was applied for further enhancement. The results show that vessel structures in hyperspectral images can be enhanced and characterized using a combination of statistical, numerical and more physics informed models.

  1. Progressive band processing for hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Schultz, Robert C.

    Hyperspectral imaging has emerged as an image processing technique in many applications. The reason that hyperspectral data is called hyperspectral is mainly because the massive amount of information provided by the hundreds of spectral bands that can be used for data analysis. However, due to very high band-to-band correlation much information may be also redundant. Consequently, how to effectively and best utilize such rich spectral information becomes very challenging. One general approach is data dimensionality reduction which can be performed by data compression techniques, such as data transforms, and data reduction techniques, such as band selection. This dissertation presents a new area in hyperspectral imaging, to be called progressive hyperspectral imaging, which has not been explored in the past. Specifically, it derives a new theory, called Progressive Band Processing (PBP) of hyperspectral data that can significantly reduce computing time and can also be realized in real-time. It is particularly suited for application areas such as hyperspectral data communications and transmission where data can be communicated and transmitted progressively through spectral or satellite channels with limited data storage. Most importantly, PBP allows users to screen preliminary results before deciding to continue with processing the complete data set. These advantages benefit users of hyperspectral data by reducing processing time and increasing the timeliness of crucial decisions made based on the data such as identifying key intelligence information when a required response time is short.

  2. Satellite Hyperspectral Imaging Simulation

    NASA Technical Reports Server (NTRS)

    Zanoni, Vicki; Stanley, Tom; Blonski, Slawomir; Cao, Changyong; Gasser, Jerry; Ryan, Robert; Zanoni, Vicki; Stanley, Tom

    1999-01-01

    Simulations of generic pushbroom satellite hyperspetral sensors have been performed to evaluate the potential performance and validation techniques for satellite systems such as COIS (NEMO), Warfighter-1 (OrbView-4), and Hyperion (EO-1). The simulaitons start with a generation of synthetic scenes form material maps of studied terrain. Scene-reflected radiance is corrected for atmospheric effects and convolved with sensor spectral response uwing MODTRAN 4 radiance and transmission calculations. Scene images are further convolved with point spread functions derived from Optical Transfer Functions (OTF's) of the sensor system. Photon noise and detector/electronics noise are added to the simulated images, which are also finally quantized to the sensor bit resolution. Studied scenes include bridges and straight roads used for evaluation of sensor spatial resolution, as well as fields of minerals, vegetation, and manmade materials used for evaluation of sensor radiometric response and sensitivity. The scenes are simulated with various seasons and weather conditions. Signal-to-noise ratos and expected performance are estimated for typica satellite system specifications and are discussed for all the scenes.

  3. Reflectance and fluorescence hyperspectral elastic image registration

    NASA Astrophysics Data System (ADS)

    Lange, Holger; Baker, Ross; Hakansson, Johan; Gustafsson, Ulf P.

    2004-05-01

    Science and Technology International (STI) presents a novel multi-modal elastic image registration approach for a new hyperspectral medical imaging modality. STI's HyperSpectral Diagnostic Imaging (HSDI) cervical instrument is used for the early detection of uterine cervical cancer. A Computer-Aided-Diagnostic (CAD) system is being developed to aid the physician with the diagnosis of pre-cancerous and cancerous tissue regions. The CAD system uses the fusion of multiple data sources to optimize its performance. The key enabling technology for the data fusion is image registration. The difficulty lies in the image registration of fluorescence and reflectance hyperspectral data due to the occurrence of soft tissue movement and the limited resemblance of these types of imagery. The presented approach is based on embedding a reflectance image in the fluorescence hyperspectral imagery. Having a reflectance image in both data sets resolves the resemblance problem and thereby enables the use of elastic image registration algorithms required to compensate for soft tissue movements. Several methods of embedding the reflectance image in the fluorescence hyperspectral imagery are described. Initial experiments with human subject data are presented where a reflectance image is embedded in the fluorescence hyperspectral imagery.

  4. Hyperspectral imaging utility for transportation systems

    NASA Astrophysics Data System (ADS)

    Bridgelall, Raj; Rafert, J. Bruce; Tolliver, Denver

    2015-03-01

    The global transportation system is massive, open, and dynamic. Existing performance and condition assessments of the complex interacting networks of roadways, bridges, railroads, pipelines, waterways, airways, and intermodal ports are expensive. Hyperspectral imaging is an emerging remote sensing technique for the non-destructive evaluation of multimodal transportation infrastructure. Unlike panchromatic, color, and infrared imaging, each layer of a hyperspectral image pixel records reflectance intensity from one of dozens or hundreds of relatively narrow wavelength bands that span a broad range of the electromagnetic spectrum. Hence, every pixel of a hyperspectral scene provides a unique spectral signature that offers new opportunities for informed decision-making in transportation systems development, operations, and maintenance. Spaceborne systems capture images of vast areas in a short period but provide lower spatial resolution than airborne systems. Practitioners use manned aircraft to achieve higher spatial and spectral resolution, but at the price of custom missions and narrow focus. The rapid size and cost reduction of unmanned aircraft systems promise a third alternative that offers hybrid benefits at affordable prices by conducting multiple parallel missions. This research formulates a theoretical framework for a pushbroom type of hyperspectral imaging system on each type of data acquisition platform. The study then applies the framework to assess the relative potential utility of hyperspectral imaging for previously proposed remote sensing applications in transportation. The authors also introduce and suggest new potential applications of hyperspectral imaging in transportation asset management, network performance evaluation, and risk assessments to enable effective and objective decision- and policy-making.

  5. Hyperspectral imaging of atherosclerotic plaques in vitro

    NASA Astrophysics Data System (ADS)

    Larsen, Eivind L. P.; Randeberg, Lise L.; Olstad, Elisabeth; Haugen, Olav A.; Aksnes, Astrid; Svaasand, Lars O.

    2011-02-01

    Vulnerable plaques constitute a risk for serious heart problems, and are difficult to identify using existing methods. Hyperspectral imaging combines spectral- and spatial information, providing new possibilities for precise optical characterization of atherosclerotic lesions. Hyperspectral data were collected from excised aorta samples (n = 11) using both white-light and ultraviolet illumination. Single lesions (n = 42) were chosen for further investigation, and classified according to histological findings. The corresponding hyperspectral images were characterized using statistical image analysis tools (minimum noise fraction, K-means clustering, principal component analysis) and evaluation of reflectance/fluorescence spectra. Image analysis combined with histology revealed the complexity and heterogeneity of aortic plaques. Plaque features such as lipids and calcifications could be identified from the hyperspectral images. Most of the advanced lesions had a central region surrounded by an outer rim or shoulder-region of the plaque, which is considered a weak spot in vulnerable lesions. These features could be identified in both the white-light and fluorescence data. Hyperspectral imaging was shown to be a promising tool for detection and characterization of advanced atherosclerotic plaques in vitro. Hyperspectral imaging provides more diagnostic information about the heterogeneity of the lesions than conventional single point spectroscopic measurements.

  6. Novel hyperspectral imager for lightweight UAVs

    NASA Astrophysics Data System (ADS)

    Saari, Heikki; Aallos, Ville-Veikko; Holmlund, Christer; Mäkynen, Jussi; Delauré, Bavo; Nackaerts, Kris; Michiels, Bart

    2010-04-01

    VTT Technical Research Centre of Finland has developed a new miniaturized staring hyperspectral imager with a weight of 350 g making the system compatible with lightweight UAS platforms. The instrument is able to record 2D spatial images at the selected wavelength bands simultaneously. The concept of the hyperspectral imager has been published in the SPIE Proc. 74741. The operational wavelength range of the imager can be tuned in the range 400 - 1100 nm and spectral resolution is in the range 5 - 10 nm @ FWHM. Presently the spatial resolution is 480 × 750 pixels but it can be increased simply by changing the image sensor. The field of view of the system is 20 × 30 degrees and ground pixel size at 100 m flying altitude is around 7.5 cm. The system contains batteries, image acquisition control system and memory for the image data. It can operate autonomously recording hyperspectral data cubes continuously or controlled by the autopilot system of the UAS. The new hyperspectral imager prototype was first tried in co-operation with the Flemish Institute for Technological Research (VITO) on their UAS helicopter. The instrument was configured for the spectral range 500 - 900 nm selected for the vegetation and natural water monitoring applications. The design of the UAS hyperspectral imager and its characterization results together with the analysis of the spectral data from first test flights will be presented.

  7. Portable Hyperspectral Imaging Broadens Sensing Horizons

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Broadband multispectral imaging can be very helpful in showing differences in energy being radiated and is often employed by NASA satellites to monitor temperature and climate changes. In addition, hyperspectral imaging is ideal for advanced laboratory uses, biomedical imaging, forensics, counter-terrorism, skin health, food safety, and Earth imaging. Lextel Intelligence Systems, LLC, of Jackson, Mississippi purchased Photon Industries Inc., a spinoff company of NASA's Stennis Space Center and the Institute for Technology Development dedicated to developing new hyperspectral imaging technologies. Lextel has added new features to and expanded the applicability of the hyperspectral imaging systems. It has made advances in the size, usability, and cost of the instruments. The company now offers a suite of turnkey hyperspectral imaging systems based on the original NASA groundwork. It currently has four lines of hyperspectral imaging products: the EagleEye VNIR 100E, the EagleEye SWIR 100E, the EagleEye SWIR 200E, and the EagleEye UV 100E. These Lextel instruments are used worldwide for a wide variety of applications including medical, military, forensics, and food safety.

  8. Uncooled long-wave infrared hyperspectral imaging

    NASA Technical Reports Server (NTRS)

    Lucey, Paul G. (Inventor)

    2006-01-01

    A long-wave infrared hyperspectral sensor device employs a combination of an interferometer with an uncooled microbolometer array camera to produce hyperspectral images without the use of bulky, power-hungry motorized components, making it suitable for UAV vehicles, small mobile platforms, or in extraterrestrial environments. The sensor device can provide signal-to-noise ratios near 200 for ambient temperature scenes with 33 wavenumber resolution at a frame rate of 50 Hz, with higher results indicated by ongoing component improvements.

  9. Real-time snapshot hyperspectral imaging endoscope

    PubMed Central

    Kester, Robert T.; Bedard, Noah; Gao, Liang; Tkaczyk, Tomasz S.

    2011-01-01

    Hyperspectral imaging has tremendous potential to detect important molecular biomarkers of early cancer based on their unique spectral signatures. Several drawbacks have limited its use for in vivo screening applications: most notably the poor temporal and spatial resolution, high expense, and low optical throughput of existing hyperspectral imagers. We present the development of a new real-time hyperspectral endoscope (called the image mapping spectroscopy endoscope) based on an image mapping technique capable of addressing these challenges. The parallel high throughput nature of this technique enables the device to operate at frame rates of 5.2 frames per second while collecting a (x, y, λ) datacube of 350 × 350 × 48. We have successfully imaged tissue in vivo, resolving a vasculature pattern of the lower lip while simultaneously detecting oxy-hemoglobin. PMID:21639573

  10. MEMS FPI-based smartphone hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Rissanen, Anna; Saari, Heikki; Rainio, Kari; Stuns, Ingmar; Viherkanto, Kai; Holmlund, Christer; Näkki, Ismo; Ojanen, Harri

    2016-05-01

    This paper demonstrates a mobile phone- compatible hyperspectral imager based on a tunable MEMS Fabry-Perot interferometer. The realized iPhone 5s hyperspectral imager (HSI) demonstrator utilizes MEMS FPI tunable filter for visible-range, which consist of atomic layer deposited (ALD) Al2O3/TiO2-thin film Bragg reflectors. Characterization results for the mobile phone hyperspectral imager utilizing MEMS FPI chip optimized for 500 nm is presented; the operation range is λ = 450 - 550 nm with FWHM between 8 - 15 nm. Also a configuration of two cascaded FPIs (λ = 500 nm and λ = 650 nm) combined with an RGB colour camera is presented. With this tandem configuration, the overall wavelength tuning range of MEMS hyperspectral imagers can be extended to cover a larger range than with a single FPI chip. The potential applications of mobile hyperspectral imagers in the vis-NIR range include authentication, counterfeit detection and potential health/wellness and food sensing applications.

  11. Compressive Hyperspectral Imaging With Side Information

    NASA Astrophysics Data System (ADS)

    Yuan, Xin; Tsai, Tsung-Han; Zhu, Ruoyu; Llull, Patrick; Brady, David; Carin, Lawrence

    2015-09-01

    A blind compressive sensing algorithm is proposed to reconstruct hyperspectral images from spectrally-compressed measurements.The wavelength-dependent data are coded and then superposed, mapping the three-dimensional hyperspectral datacube to a two-dimensional image. The inversion algorithm learns a dictionary {\\em in situ} from the measurements via global-local shrinkage priors. By using RGB images as side information of the compressive sensing system, the proposed approach is extended to learn a coupled dictionary from the joint dataset of the compressed measurements and the corresponding RGB images, to improve reconstruction quality. A prototype camera is built using a liquid-crystal-on-silicon modulator. Experimental reconstructions of hyperspectral datacubes from both simulated and real compressed measurements demonstrate the efficacy of the proposed inversion algorithm, the feasibility of the camera and the benefit of side information.

  12. Tongue Tumor Detection in Medical Hyperspectral Images

    PubMed Central

    Liu, Zhi; Wang, Hongjun; Li, Qingli

    2012-01-01

    A hyperspectral imaging system to measure and analyze the reflectance spectra of the human tongue with high spatial resolution is proposed for tongue tumor detection. To achieve fast and accurate performance for detecting tongue tumors, reflectance data were collected using spectral acousto-optic tunable filters and a spectral adapter, and sparse representation was used for the data analysis algorithm. Based on the tumor image database, a recognition rate of 96.5% was achieved. The experimental results show that hyperspectral imaging for tongue tumor diagnosis, together with the spectroscopic classification method provide a new approach for the noninvasive computer-aided diagnosis of tongue tumors. PMID:22368462

  13. Hyperspectral imager development at Army Research Laboratory

    NASA Astrophysics Data System (ADS)

    Gupta, Neelam

    2008-04-01

    Development of robust compact optical imagers that can acquire both spectral and spatial features from a scene of interest is of utmost importance for standoff detection of chemical and biological agents as well as targets and backgrounds. Spectral features arise due to the material properties of objects as a result of the emission, reflection, and absorption of light. Using hyperspectral imaging one can acquire images with narrow spectral bands and take advantage of the characteristic spectral signatures of different materials making up the scene in detection of objects. Traditional hyperspectral imaging systems use gratings and prisms that acquire one-dimensional spectral images and require relative motion of sensor and scene in addition to data processing to form a two-dimensional image cube. There is much interest in developing hyperspectral imagers using tunable filters that acquire a two-dimensional spectral image and build up an image cube as a function of time. At the Army Research Laboratory (ARL), we are developing hyperspectral imagers using a number of novel tunable filter technologies. These include acousto-optic tunable filters (AOTFs) that can provide adaptive no-moving-parts imagers from the UV to the long wave infrared, diffractive optics technology that can provide image cubes either in a single spectral region or simultaneously in different spectral regions using a single moving lens or by using a lenslet array, and micro-electromechanical systems (MEMS)-based Fabry-Perot (FP) tunable etalons to develop miniature sensors that take advantage of the advances in microfabrication and packaging technologies. New materials are being developed to design AOTFs and a full Stokes polarization imager has been developed, diffractive optics lenslet arrays are being explored, and novel FP tunable filters are under fabrication for the development of novel miniature hyperspectral imagers. Here we will brief on all the technologies being developed and present

  14. Phase congruency assesses hyperspectral image quality

    NASA Astrophysics Data System (ADS)

    Shao, Xiaopeng; Zhong, Cheng

    2012-10-01

    Blind image quality assessment (QA) is a tough task especially for hyperspectral imagery which is degraded by noise, distortion, defocus, and other complex factors. Subjective hyperspectral imagery QA methods are basically measured the degradation of image from human perceptual visual quality. As the most important image quality measurement features, noise and blur, determined the image quality greatly, are employed to predict the objective hyperspectral imagery quality of each band. We demonstrate a novel no-reference hyperspectral imagery QA model based on phase congruency (PC), which is a dimensionless quantity and provides an absolute measure of the significance of feature point. First, Log Gabor wavelet is used to calculate the phase congruency of frequencies of each band image. The relationship between noise and PC can be derived from above transformation under the assumption that noise is additive. Second, PC focus measure evaluation model is proposed to evaluate blur caused by different amounts of defocus. The ratio and mean factors of edge blur level and noise is defined to assess the quality of each band image. This image QA method obtains excellent correlation with subjective image quality score without any reference. Finally, the PC information is utilized to improve the quality of some bands images.

  15. Hyperspectral image feature extraction accelerated by GPU

    NASA Astrophysics Data System (ADS)

    Qu, HaiCheng; Zhang, Ye; Lin, Zhouhan; Chen, Hao

    2012-10-01

    PCA (principal components analysis) algorithm is the most basic method of dimension reduction for high-dimensional data1, which plays a significant role in hyperspectral data compression, decorrelation, denoising and feature extraction. With the development of imaging technology, the number of spectral bands in a hyperspectral image is getting larger and larger, and the data cube becomes bigger in these years. As a consequence, operation of dimension reduction is more and more time-consuming nowadays. Fortunately, GPU-based high-performance computing has opened up a novel approach for hyperspectral data processing6. This paper is concerning on the two main processes in hyperspectral image feature extraction: (1) calculation of transformation matrix; (2) transformation in spectrum dimension. These two processes belong to computationally intensive and data-intensive data processing respectively. Through the introduction of GPU parallel computing technology, an algorithm containing PCA transformation based on eigenvalue decomposition 8(EVD) and feature matching identification is implemented, which is aimed to explore the characteristics of the GPU parallel computing and the prospects of GPU application in hyperspectral image processing by analysing thread invoking and speedup of the algorithm. At last, the result of the experiment shows that the algorithm has reached a 12x speedup in total, in which some certain step reaches higher speedups up to 270 times.

  16. Standoff Hyperspectral Imaging of Explosives Residues Using Broadly Tunable External Cavity Quantum Cascade Laser Illumination

    SciTech Connect

    Bernacki, Bruce E.; Phillips, Mark C.

    2010-05-01

    We describe experimental results on the detection of explosives residues using active hyperspectral imaging by illumination of the target surface using an external cavity quantum cascade laser (ECQCL) and imaging using a room temperature microbolometer camera. The active hyperspectral imaging technique forms an image hypercube by recording one image for each tuning step of the ECQCL. The resulting hyperspectral image contains the full absorption spectrum produced by the illumination laser at each pixel in the image which can then be used to identify the explosive type and relative quantity using spectral identification approaches developed initially in the remote sensing community.

  17. Hyperspectral imaging of melanocytic lesions.

    PubMed

    Gaudi, Sudeep; Meyer, Rebecca; Ranka, Jayshree; Granahan, James C; Israel, Steven A; Yachik, Theodore R; Jukic, Drazen M

    2014-02-01

    Hyperspectral imaging (HSI) allows the identification of objects through the analysis of their unique spectral signatures. Although first developed many years ago for use in terrestrial remote sensing, this technology has more recently been studied for application in the medical field. With preliminary data favoring a role for HSI in distinguishing normal and lesional skin tissues, we sought to investigate the potential use of HSI as a diagnostic aid in the classification of atypical Spitzoid neoplasms, a group of lesions that often leave dermatopathologists bewildered. One hundred and two hematoxylin and eosin-stained tissue samples were divided into 1 of 4 diagnostic categories (Spitz nevus, Spitz nevus with unusual features, atypical Spitzoid neoplasm, and Spitzoid malignant melanoma) and 1 of 2 control groups (benign melanocytic nevus and malignant melanoma). A region of interest was selected from the dermal component of each sample, thereby maximizing the examination of melanocytes. Tissue samples were examined at ×400 magnification using a spectroscopy system interfaced with a light microscope. The absorbance patterns of wavelengths from 385 to 880 nm were measured and then analyzed within and among groups. All tissue groups demonstrated 3 common absorbance spectra at 496, 533, and 838 nm. Each sample group contained at least one absorption point that was unique to that group. The Spitzoid malignant melanoma category had the highest number of total and unique absorption points for any sample group. The data were then clustered into 12 representative spectral classes. Although each of the sample groups contained all 12 spectral vectors, they did so in differing proportions. These preliminary results reveal differences in the spectral signatures of the Spitzoid lesions examined in this study. Further investigation into a role for HSI in classifying atypical Spitzoid neoplasms is encouraged. PMID:24247577

  18. Dynamical Spectral Unmixing of Multitemporal Hyperspectral Images

    NASA Astrophysics Data System (ADS)

    Henrot, Simon; Chanussot, Jocelyn; Jutten, Christian

    2016-07-01

    In this paper, we consider the problem of unmixing a time series of hyperspectral images. We propose a dynamical model based on linear mixing processes at each time instant. The spectral signatures and fractional abundances of the pure materials in the scene are seen as latent variables, and assumed to follow a general dynamical structure. Based on a simplified version of this model, we derive an efficient spectral unmixing algorithm to estimate the latent variables by performing alternating minimizations. The performance of the proposed approach is demonstrated on synthetic and real multitemporal hyperspectral images.

  19. Mid-infrared, broadly tunable, active hyperspectral imaging system for the detection of gaseous hydrocarbon species

    NASA Astrophysics Data System (ADS)

    Stothard, David J. M.; Rae, Cameron F.; Ross, Mark; Dunn, Malcolm H.

    2007-10-01

    We describe a compact mid-infrared active spectroscopic imaging system for the rapid, stand-off detection of gas / chemical agents. Based upon the back-scatter absorption gas imaging (BAGI) technique, the system utilises a miniaturised, extremely efficient all solid state intracavity optical parametric oscillator (OPO) as the imaging illumination source. The OPO produces up to 250mW of tunable radiation in the signal and idler fields over the range 1.3-4.5μm, for a diode pump power of only 3W. Due to the nature of the nonlinear crystal employed within the OPO, the system can be tuned across its spectral range in ~1 second. We obviate the very high cost and complexity of a cooled MCT or InSb video array by raster-scanning the collimated illumination beam over the area of interest and reconstructing the image by sampling the back-scattered radiation with a single element MCT photo-detector at each pixel point. This approach also improves the ultimate signal to noise ratio. Video-like frame rates of 10 f.p.s. have been demonstrated via this technique. The range limit of the instrument is currently <10 meters which is limited by the detector we currently employ. We demonstrate how the system has been used to detect, in real time, leaks of multi-species hydrocarbon gases.

  20. Research on hyperspectral polarization imaging technique

    NASA Astrophysics Data System (ADS)

    Zhao, Haibo; Feng, Lei; Zhou, Yu; Wang, Zheng; Lin, Xuling

    2015-08-01

    The summary of hyperspectral polarization remote sensing detection is presented, including the characteristics and mechanism of polarization detection, the expression of polarization light and the detection method. The present research of hyperspectral polarization remote sensing is introduced. A novel method of hyperspectral polarization imaging technique is discussed, which is based on static modulation adding with the double refraction crystal. The static modulation is composed of one polarizer and two retarders. The double refraction crystal is used to generate interference image. The four Stokes vectors and spectral information can be detected only by one measurement. The method of static modulation is introduced in detail and is simulated by computer. The experimental system is also established in laboratory. The basic concept of the technique is verified. The simulation error of DOP (polarization degree detection) is about 1%. The experimental error of DOP is less than 5%. The merits of the novel system are no moving parts, compactness and no electrical element.

  1. Landmine detection using passive hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    McFee, John E.; Anger, Cliff; Achal, Steve; Ivanco, Tyler

    2007-04-01

    Airborne hyperspectral imaging has been studied since the late 1980s as a tool to detect minefields for military countermine operations and for level I clearance for humanitarian demining. Hyperspectral imaging employed on unmanned ground vehicles may also be used to augment or replace broadband imagers to detect individual mines. This paper will discuss the ability of different optical wavebands - the visible/near infrared (VNIR), shortwave infrared (SWIR) and thermal infrared (TIR) - to detect surface-laid and buried mines. The phenomenology that determines performance in the different bands is discussed. Hyperspectral imagers have usually been designed and built for general purpose remote sensing applications and often do not meet the requirements of mine detection. The DRDC mine detection research program has sponsored the development by Itres Research of VNIR, SWIR and TIR instruments specifically intended for mine detection. The requirements for such imagers are described, as well as the instruments. Some results of mine detection experiments are presented. To date, reliable day time detection of surface-laid mines in non-real-time, independent of solar angle, time of day and season has been demonstrated in the VNIR and SWIR. Real-time analysis, necessary for military applications, has been demonstrated from low speed ground vehicles and recently from airborne platforms. Reliable, repeatable detection of buried mines has yet to be demonstrated, although a recently completed TIR hyperspectral imager will soon be tested for such a capability.

  2. Unsupervised linear unmixing of hyperspectral image for crop yield estimation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Multispectral and hyperspectral imagery are often used for estimating crop yield. This paper describes an unsupervised unmixing scheme of hyperspectral images to estimate crop yield. From the hyperspectral images, the endmembers and their abundance maps are computed by unsupervised unmixing. The abu...

  3. Biometric study using hyperspectral imaging during stress

    NASA Astrophysics Data System (ADS)

    Nagaraj, Sheela; Quoraishee, Shafik; Chan, Gabriel; Short, Kenneth R.

    2010-04-01

    To the casual observer, transient stress results in a variety of physiological changes that can be seen in the face. Although the conditions can be seen visibly, the conditions affect the emissivity and absorption properties of the skin, which imaging spectrometers, commonly referred to as Hyperspectral (HS) cameras, can quantify at every image pixel. The study reported on in this paper, using Hyperspectral cameras, provides a basis for continued study of HS imaging to eventually quantify biometric stress. This study was limited to the visible to near infrared (VNIR) spectral range. Signal processing tools and algorithms have been developed and are described for using HS face data from human subjects. The subjects were placed in psychologically stressful situations and the camera data were analyzed to detect stress through changes in dermal reflectance and emissivity. Results indicate that hyperspectral imaging may potentially serve as a non-invasive tool to measure changes in skin emissivity indicative of a stressful incident. Particular narrow spectral bands in the near-infrared region of the electromagnetic spectrum seem especially important. Further studies need to be performed to determine the optimal spectral bands and to generalize the conclusions. The enormous information available in hyperspectral imaging needs further analysis and more spectral regions need to be exploited. Non-invasive stress detection is a prominent area of research with countless applications for both military and commercial use including border patrol, stand-off interrogation, access control, surveillance, and non-invasive and un-attended patient monitoring.

  4. Emissivity retrieval from indoor hyperspectral imaging of mineral grains

    NASA Astrophysics Data System (ADS)

    Yousefi, Bardia; Sojasi, Saeed; Ibarra Castanedo, Clemente; Beaudoin, Georges; Huot, François; Maldague, Xavier P. V.; Chamberland, Martin; Lalonde, Erik

    2016-05-01

    The proposed approach addresses the problem of retrieving the emissivity of hyperspectral data in the spectroscopic imageries from indoor experiments. This methodology was tested on experimental data that have been recorded with hyperspectral images working in visible/near infrared and long-wave infrared bands. The proposed technique provides a framework for computing down-welling spectral radiance applying non-negative matrix factorization (NMF) analysis. It provides the necessary means for the non-uniform correction of active thermographical experiments. The obtained results indicate promising accuracy. In addition, the application of the proposed technique is not limited to non-uniform heating spectroscopy but to uniform spectroscopy as well.

  5. Quality evaluation of fruit by hyperspectral imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This chapter presents new applications of hyperspectral imaging for measuring the optical properties of fruits and assessing their quality attributes. A brief overview is given of current techniques for measuring optical properties of turbid and opaque biological materials. Then a detailed descripti...

  6. LIFTERS-hyperspectral imaging at LLNL

    SciTech Connect

    Fields, D.; Bennett, C.; Carter, M.

    1994-11-15

    LIFTIRS, the Livermore Imaging Fourier Transform InfraRed Spectrometer, recently developed at LLNL, is an instrument which enables extremely efficient collection and analysis of hyperspectral imaging data. LIFTIRS produces a spatial format of 128x128 pixels, with spectral resolution arbitrarily variable up to a maximum of 0.25 inverse centimeters. Time resolution and spectral resolution can be traded off for each other with great flexibility. We will discuss recent measurements made with this instrument, and present typical images and spectra.

  7. Metric Learning to Enhance Hyperspectral Image Segmentation

    NASA Technical Reports Server (NTRS)

    Thompson, David R.; Castano, Rebecca; Bue, Brian; Gilmore, Martha S.

    2013-01-01

    Unsupervised hyperspectral image segmentation can reveal spatial trends that show the physical structure of the scene to an analyst. They highlight borders and reveal areas of homogeneity and change. Segmentations are independently helpful for object recognition, and assist with automated production of symbolic maps. Additionally, a good segmentation can dramatically reduce the number of effective spectra in an image, enabling analyses that would otherwise be computationally prohibitive. Specifically, using an over-segmentation of the image instead of individual pixels can reduce noise and potentially improve the results of statistical post-analysis. In this innovation, a metric learning approach is presented to improve the performance of unsupervised hyperspectral image segmentation. The prototype demonstrations attempt a superpixel segmentation in which the image is conservatively over-segmented; that is, the single surface features may be split into multiple segments, but each individual segment, or superpixel, is ensured to have homogenous mineralogy.

  8. ICER-3D Hyperspectral Image Compression Software

    NASA Technical Reports Server (NTRS)

    Xie, Hua; Kiely, Aaron; Klimesh, matthew; Aranki, Nazeeh

    2010-01-01

    Software has been developed to implement the ICER-3D algorithm. ICER-3D effects progressive, three-dimensional (3D), wavelet-based compression of hyperspectral images. If a compressed data stream is truncated, the progressive nature of the algorithm enables reconstruction of hyperspectral data at fidelity commensurate with the given data volume. The ICER-3D software is capable of providing either lossless or lossy compression, and incorporates an error-containment scheme to limit the effects of data loss during transmission. The compression algorithm, which was derived from the ICER image compression algorithm, includes wavelet-transform, context-modeling, and entropy coding subalgorithms. The 3D wavelet decomposition structure used by ICER-3D exploits correlations in all three dimensions of sets of hyperspectral image data, while facilitating elimination of spectral ringing artifacts, using a technique summarized in "Improving 3D Wavelet-Based Compression of Spectral Images" (NPO-41381), NASA Tech Briefs, Vol. 33, No. 3 (March 2009), page 7a. Correlation is further exploited by a context-modeling subalgorithm, which exploits spectral dependencies in the wavelet-transformed hyperspectral data, using an algorithm that is summarized in "Context Modeler for Wavelet Compression of Hyperspectral Images" (NPO-43239), which follows this article. An important feature of ICER-3D is a scheme for limiting the adverse effects of loss of data during transmission. In this scheme, as in the similar scheme used by ICER, the spatial-frequency domain is partitioned into rectangular error-containment regions. In ICER-3D, the partitions extend through all the wavelength bands. The data in each partition are compressed independently of those in the other partitions, so that loss or corruption of data from any partition does not affect the other partitions. Furthermore, because compression is progressive within each partition, when data are lost, any data from that partition received

  9. Hyperspectral imaging using RGB color for foodborne pathogen detection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper reports the latest development of a color vision technique for detecting colonies of foodborne pathogens grown on agar plates with a hyperspectral image classification model that was developed using full hyperspectral data. The hyperspectral classification model depended on reflectance sp...

  10. Mapping Soil Organic Matter with Hyperspectral Imaging

    NASA Astrophysics Data System (ADS)

    Moni, Christophe; Burud, Ingunn; Flø, Andreas; Rasse, Daniel

    2014-05-01

    Soil organic matter (SOM) plays a central role for both food security and the global environment. Soil organic matter is the 'glue' that binds soil particles together, leading to positive effects on soil water and nutrient availability for plant growth and helping to counteract the effects of erosion, runoff, compaction and crusting. Hyperspectral measurements of samples of soil profiles have been conducted with the aim of mapping soil organic matter on a macroscopic scale (millimeters and centimeters). Two soil profiles have been selected from the same experimental site, one from a plot amended with biochar and another one from a control plot, with the specific objective to quantify and map the distribution of biochar in the amended profile. The soil profiles were of size (30 x 10 x 10) cm3 and were scanned with two pushbroomtype hyperspectral cameras, one which is sensitive in the visible wavelength region (400 - 1000 nm) and one in the near infrared region (1000 - 2500 nm). The images from the two detectors were merged together into one full dataset covering the whole wavelength region. Layers of 15 mm were removed from the 10 cm high sample such that a total of 7 hyperspectral images were obtained from the samples. Each layer was analyzed with multivariate statistical techniques in order to map the different components in the soil profile. Moreover, a 3-dimensional visalization of the components through the depth of the sample was also obtained by combining the hyperspectral images from all the layers. Mid-infrared spectroscopy of selected samples of the measured soil profiles was conducted in order to correlate the chemical constituents with the hyperspectral results. The results show that hyperspectral imaging is a fast, non-destructive technique, well suited to characterize soil profiles on a macroscopic scale and hence to map elements and different organic matter quality present in a complete pedon. As such, we were able to map and quantify biochar in our

  11. Nonnegative Matrix Factorization for Efficient Hyperspectral Image Projection

    NASA Technical Reports Server (NTRS)

    Iacchetta, Alexander S.; Fienup, James R.; Leisawitz, David T.; Bolcar, Matthew R.

    2015-01-01

    Hyperspectral imaging for remote sensing has prompted development of hyperspectral image projectors that can be used to characterize hyperspectral imaging cameras and techniques in the lab. One such emerging astronomical hyperspectral imaging technique is wide-field double-Fourier interferometry. NASA's current, state-of-the-art, Wide-field Imaging Interferometry Testbed (WIIT) uses a Calibrated Hyperspectral Image Projector (CHIP) to generate test scenes and provide a more complete understanding of wide-field double-Fourier interferometry. Given enough time, the CHIP is capable of projecting scenes with astronomically realistic spatial and spectral complexity. However, this would require a very lengthy data collection process. For accurate but time-efficient projection of complicated hyperspectral images with the CHIP, the field must be decomposed both spectrally and spatially in a way that provides a favorable trade-off between accurately projecting the hyperspectral image and the time required for data collection. We apply nonnegative matrix factorization (NMF) to decompose hyperspectral astronomical datacubes into eigenspectra and eigenimages that allow time-efficient projection with the CHIP. Included is a brief analysis of NMF parameters that affect accuracy, including the number of eigenspectra and eigenimages used to approximate the hyperspectral image to be projected. For the chosen field, the normalized mean squared synthesis error is under 0.01 with just 8 eigenspectra. NMF of hyperspectral astronomical fields better utilizes the CHIP's capabilities, providing time-efficient and accurate representations of astronomical scenes to be imaged with the WIIT.

  12. Hyperspectral image data compression based on DSP

    NASA Astrophysics Data System (ADS)

    Fan, Jiming; Zhou, Jiankang; Chen, Xinhua; Shen, Weimin

    2010-11-01

    The huge data volume of hyperspectral image challenges its transportation and store. It is necessary to find an effective method to compress the hyperspectral image. Through analysis and comparison of current various algorithms, a mixed compression algorithm based on prediction, integer wavelet transform and embedded zero-tree wavelet (EZW) is proposed in this paper. We adopt a high-powered Digital Signal Processor (DSP) of TMS320DM642 to realize the proposed algorithm. Through modifying the mixed algorithm and optimizing its algorithmic language, the processing efficiency of the program was significantly improved, compared the non-optimized one. Our experiment show that the mixed algorithm based on DSP runs much faster than the algorithm on personal computer. The proposed method can achieve the nearly real-time compression with excellent image quality and compression performance.

  13. Hyperspectral Imaging for Cancer Surgical Margin Delineation: Registration of Hyperspectral and Histological Images

    PubMed Central

    Lu, Guolan; Halig, Luma; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

    2014-01-01

    The determination of tumor margins during surgical resection remains a challenging task. A complete removal of malignant tissue and conservation of healthy tissue is important for the preservation of organ function, patient satisfaction, and quality of life. Visual inspection and palpation is not sufficient for discriminating between malignant and normal tissue types. Hyperspectral imaging (HSI) technology has the potential to noninvasively delineate surgical tumor margin and can be used as an intra-operative visual aid tool. Since histological images provide the ground truth of cancer margins, it is necessary to warp the cancer regions in ex vivo histological images back to in vivo hyperspectral images in order to validate the tumor margins detected by HSI and to optimize the imaging parameters. In this paper, principal component analysis (PCA) is utilized to extract the principle component bands of the HSI images, which is then used to register HSI images with the corresponding histological image. Affine registration is chosen to model the global transformation. A B-spline free form deformation (FFD) method is used to model the local non-rigid deformation. Registration experiment was performed on animal hyperspectral and histological images. Experimental results from animals demonstrated the feasibility of the hyperspectral imaging method for cancer margin detection. PMID:25328640

  14. Hyperspectral imaging for cancer surgical margin delineation: registration of hyperspectral and histological images

    NASA Astrophysics Data System (ADS)

    Lu, Guolan; Halig, Luma; Wang, Dongsheng; Chen, Zhuo G.; Fei, Baowei

    2014-03-01

    The determination of tumor margins during surgical resection remains a challenging task. A complete removal of malignant tissue and conservation of healthy tissue is important for the preservation of organ function, patient satisfaction, and quality of life. Visual inspection and palpation is not sufficient for discriminating between malignant and normal tissue types. Hyperspectral imaging (HSI) technology has the potential to noninvasively delineate surgical tumor margin and can be used as an intra-operative visual aid tool. Since histological images provide the ground truth of cancer margins, it is necessary to warp the cancer regions in ex vivo histological images back to in vivo hyperspectral images in order to validate the tumor margins detected by HSI and to optimize the imaging parameters. In this paper, principal component analysis (PCA) is utilized to extract the principle component bands of the HSI images, which is then used to register HSI images with the corresponding histological image. Affine registration is chosen to model the global transformation. A B-spline free form deformation (FFD) method is used to model the local non-rigid deformation. Registration experiment was performed on animal hyperspectral and histological images. Experimental results from animals demonstrated the feasibility of the hyperspectral imaging method for cancer margin detection.

  15. Hyperspectral Imaging for Cancer Surgical Margin Delineation: Registration of Hyperspectral and Histological Images.

    PubMed

    Lu, Guolan; Halig, Luma; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

    2014-03-12

    The determination of tumor margins during surgical resection remains a challenging task. A complete removal of malignant tissue and conservation of healthy tissue is important for the preservation of organ function, patient satisfaction, and quality of life. Visual inspection and palpation is not sufficient for discriminating between malignant and normal tissue types. Hyperspectral imaging (HSI) technology has the potential to noninvasively delineate surgical tumor margin and can be used as an intra-operative visual aid tool. Since histological images provide the ground truth of cancer margins, it is necessary to warp the cancer regions in ex vivo histological images back to in vivo hyperspectral images in order to validate the tumor margins detected by HSI and to optimize the imaging parameters. In this paper, principal component analysis (PCA) is utilized to extract the principle component bands of the HSI images, which is then used to register HSI images with the corresponding histological image. Affine registration is chosen to model the global transformation. A B-spline free form deformation (FFD) method is used to model the local non-rigid deformation. Registration experiment was performed on animal hyperspectral and histological images. Experimental results from animals demonstrated the feasibility of the hyperspectral imaging method for cancer margin detection. PMID:25328640

  16. Software for Simulation of Hyperspectral Images

    NASA Technical Reports Server (NTRS)

    Richtsmeier, Steven C.; Singer-Berk, Alexander; Bernstein, Lawrence S.

    2002-01-01

    A package of software generates simulated hyperspectral images for use in validating algorithms that generate estimates of Earth-surface spectral reflectance from hyperspectral images acquired by airborne and spaceborne instruments. This software is based on a direct simulation Monte Carlo approach for modeling three-dimensional atmospheric radiative transport as well as surfaces characterized by spatially inhomogeneous bidirectional reflectance distribution functions. In this approach, 'ground truth' is accurately known through input specification of surface and atmospheric properties, and it is practical to consider wide variations of these properties. The software can treat both land and ocean surfaces and the effects of finite clouds with surface shadowing. The spectral/spatial data cubes computed by use of this software can serve both as a substitute for and a supplement to field validation data.

  17. Advances in hyperspectral LWIR pushbroom imagers

    NASA Astrophysics Data System (ADS)

    Holma, Hannu; Mattila, Antti-Jussi; Hyvärinen, Timo; Weatherbee, Oliver

    2011-06-01

    Two long-wave infrared (LWIR) hyperspectral imagers have been under extensive development. The first one utilizes a microbolometer focal plane array (FPA) and the second one is based on an Mercury Cadmium Telluride (MCT) FPA. Both imagers employ a pushbroom imaging spectrograph with a transmission grating and on-axis optics. The main target has been to develop high performance instruments with good image quality and compact size for various industrial and remote sensing application requirements. A big challenge in realizing these goals without considerable cooling of the whole instrument is to control the instrument radiation. The challenge is much bigger in a hyperspectral instrument than in a broadband camera, because the optical signal from the target is spread spectrally, but the instrument radiation is not dispersed. Without any suppression, the instrument radiation can overwhelm the radiation from the target even by 1000 times. The means to handle the instrument radiation in the MCT imager include precise instrument temperature stabilization (but not cooling), efficient optical background suppression and the use of background-monitoring-on-chip (BMC) method. This approach has made possible the implementation of a high performance, extremely compact spectral imager in the 7.7 to 12.4 μm spectral range. The imager performance with 84 spectral bands and 384 spatial pixels has been experimentally verified and an excellent NESR of 14 mW/(m2srμm) at 10 μm wavelength with a 300 K target has been achieved. This results in SNR of more than 700. The LWIR imager based on a microbolometer detector array, first time introduced in 2009, has been upgraded. The sensitivity of the imager has improved drastically by a factor of 3 and SNR by about 15 %. It provides a rugged hyperspectral camera for chemical imaging applications in reflection mode in laboratory and industry.

  18. Synergetics Framework for Hyperspectral Image Classification

    NASA Astrophysics Data System (ADS)

    Müller, R.; Cerra, D.; Reinartz, P.

    2013-05-01

    In this paper a new classification technique for hyperspectral data based on synergetics theory is presented. Synergetics - originally introduced by the physicist H. Haken - is an interdisciplinary theory to find general rules for pattern formation through selforganization and has been successfully applied in fields ranging from biology to ecology, chemistry, cosmology, and thermodynamics up to sociology. Although this theory describes general rules for pattern formation it was linked also to pattern recognition. Pattern recognition algorithms based on synergetics theory have been applied to images in the spatial domain with limited success in the past, given their dependence on the rotation, shifting, and scaling of the images. These drawbacks can be discarded if such methods are applied to data acquired by a hyperspectral sensor in the spectral domain, as each single spectrum, related to an image element in the hyperspectral scene, can be analysed independently. The classification scheme based on synergetics introduces also methods for spatial regularization to get rid of "salt and pepper" classification results and for iterative parameter tuning to optimize class weights. The paper reports an experiment on a benchmark data set frequently used for method comparisons. This data set consists of a hyperspectral scene acquired by the Airborne Visible Infrared Imaging Spectrometer AVIRIS sensor of the Jet Propulsion Laboratory acquired over the Salinas Valley in CA, USA, with 15 vegetation classes. The results are compared to state-of-the-art methodologies like Support Vector Machines (SVM), Spectral Information Divergence (SID), Neural Networks, Logistic Regression, Factor Graphs or Spectral Angle Mapper (SAM). The outcomes are promising and often outperform state-of-the-art classification methodologies.

  19. Quantification and threshold detection in real-time hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Driver, Richard D.

    2009-05-01

    The technical challenges of applying hyperspectral imaging techniques to on-line real-time food monitoring is discussed. System optimization must be applied to the design of the hyperspectral imaging spectrograph, the choice and operation of the imaging detector, the design of the illumination system and finally the development of software algorithms to correctly quantify the hyperspectral images. The signal to noise limitation of hyperspectral detection is discussed with particular emphasis on the detection of moving objects at high measurement bandwidths. An example is given of the development of a simple but accurate algorithm for the detection and discrimination of rust particles on leaves.

  20. Excitation-scanning hyperspectral imaging microscope.

    PubMed

    Favreau, Peter F; Hernandez, Clarissa; Heaster, Tiffany; Alvarez, Diego F; Rich, Thomas C; Prabhat, Prashant; Leavesley, Silas J

    2014-04-01

    Hyperspectral imaging is a versatile tool that has recently been applied to a variety of biomedical applications, notably live-cell and whole-tissue signaling. Traditional hyperspectral imaging approaches filter the fluorescence emission over a broad wavelength range while exciting at a single band. However, these emission-scanning approaches have shown reduced sensitivity due to light attenuation from spectral filtering. Consequently, emission scanning has limited applicability for time-sensitive studies and photosensitive applications. In this work, we have developed an excitation-scanning hyperspectral imaging microscope that overcomes these limitations by providing high transmission with short acquisition times. This is achieved by filtering the fluorescence excitation rather than the emission. We tested the efficacy of the excitation-scanning microscope in a side-by-side comparison with emission scanning for detection of green fluorescent protein (GFP)-expressing endothelial cells in highly autofluorescent lung tissue. Excitation scanning provided higher signal-to-noise characteristics, as well as shorter acquisition times (300  ms/wavelength band with excitation scanning versus 3  s/wavelength band with emission scanning). Excitation scanning also provided higher delineation of nuclear and cell borders, and increased identification of GFP regions in highly autofluorescent tissue. These results demonstrate excitation scanning has utility in a wide range of time-dependent and photosensitive applications. PMID:24727909

  1. Information efficiency in hyperspectral imaging systems

    NASA Astrophysics Data System (ADS)

    Reichenbach, Stephen E.; Cao, Luyin; Narayanan, Ram M.

    2002-07-01

    In this work we develop a method for assessing the information density and efficiency of hyperspectral imaging systems that have spectral bands of nonuniform width. Imaging system designs with spectral bands of nonuniform width can efficiently gather information about a scene by allocating bandwidth among the bands according to their information content. The information efficiency is the ratio of information density to data density and is a function of the scene's spectral radiance, hyperspectral system design, and signal-to-noise ratio. The assessment can be used to produce an efficient system design. For example, one approach to determining the number and width of the spectral bands for an information-efficient design is to begin with a design that has a single band and then to iteratively divide a band into two bands until no further division improves the system's efficiency. Two experiments illustrate this approach, one using a simple mathematical model for the scene spectral-radiance autocorrelation function and the other using the deterministic spectral-radiance autocorrelation function of a hyperspectral image from NASA's Advanced Solid-State Array Spectroradiometer. The approach could be used either to determine a fixed system design or to dynamically control a system with variable-width spectral bands (e.g., using on-board processing in a satellite system).

  2. Hyperspectral imaging of skin and lung cancers

    NASA Astrophysics Data System (ADS)

    Zherdeva, Larisa A.; Bratchenko, Ivan A.; Alonova, Marina V.; Myakinin, Oleg O.; Artemyev, Dmitry N.; Moryatov, Alexander A.; Kozlov, Sergey V.; Zakharov, Valery P.

    2016-04-01

    The problem of cancer control requires design of new approaches for instrumental diagnostics, as the accuracy of cancer detection on the first step of diagnostics in clinics is slightly more than 50%. In this study, we present a method of visualization and diagnostics of skin and lung tumours based on registration and processing of tissues hyperspectral images. In a series of experiments registration of hyperspectral images of skin and lung tissue samples is carried out. Melanoma, basal cell carcinoma, nevi and benign tumours are studied in skin ex vivo and in vivo experiments; adenocarcinomas and squamous cell carcinomas are studied in ex vivo lung experiments. In a series of experiments the typical features of diffuse reflection spectra for pathological and normal tissues were found. Changes in tissues morphology during the tumour growth lead to the changes of blood and pigments concentration, such as melanin in skin. That is why tumours and normal tissues maybe differentiated with information about spectral response in 500-600 nm and 600 - 670 nm areas. Thus, hyperspectral imaging in the visible region may be a useful tool for cancer detection as it helps to estimate spectral properties of tissues and determine malignant regions for precise resection of tumours.

  3. Hyperspectral Transformation from EO-1 ALI Imagery Using Pseudo-Hyperspectral Image Synthesis Algorithm

    NASA Astrophysics Data System (ADS)

    Tien Hoang, Nguyen; Koike, Katsuaki

    2016-06-01

    Hyperspectral remote sensing is more effective than multispectral remote sensing in many application fields because of having hundreds of observation bands with high spectral resolution. However, hyperspectral remote sensing resources are limited both in temporal and spatial coverage. Therefore, simulation of hyperspectral imagery from multispectral imagery with a small number of bands must be one of innovative topics. Based on this background, we have recently developed a method, Pseudo-Hyperspectral Image Synthesis Algorithm (PHISA), to transform Landsat imagery into hyperspectral imagery using the correlation of reflectance at the corresponding bands between Landsat and EO-1 Hyperion data. This study extends PHISA to simulate pseudo-hyperspectral imagery from EO-1 ALI imagery. The pseudo-hyperspectral imagery has the same number of bands as that of high-quality Hyperion bands and the same swath width as ALI scene. The hyperspectral reflectance data simulated from the ALI data show stronger correlation with the original Hyperion data than the one simulated from Landsat data. This high correlation originates from the concurrent observation by the ALI and Hyperion sensors that are on-board the same satellite. The accuracy of simulation results are verified by a statistical analysis and a surface mineral mapping. With a combination of the advantages of both ALI and Hyperion image types, the pseudo-hyperspectral imagery is proved to be useful for detailed identification of minerals for the areas outside the Hyperion coverage.

  4. Unsupervised data fusion for hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Jimenez-Rodriguez, Luis O.; Velez-Reyes, Miguel; Rivera-Medina, Jorge; Velasquez, Hector

    2002-01-01

    Hyperspectral images contain a great amount of information in terms of hundreds of narrowband channels. This should lead to better parameter estimation and to more accurate classifications. However, traditional classification methods based on multispectral analysis fail to work properly on this type of data. High dimensional space poses a difficulty in obtaining accurate parameter estimates and as a consequence this makes unsupervised classification a challenge that requires new techniques. Thus, alternative methods are needed to take advantage of the information provided by the hyperdimensional data. Data fusion is an alternative when dealing with such large data sets in order to improve classification accuracy. Data fusion is an important process in the areas of environmental systems, surveillance, automation, medical imaging, and robotics. The uses of this technique in Remote Sensing have been recently expanding. A relevant application is to adapt the data fusion approaches to be used on hyperspectral imagery taking into consideration the special characteristics of such data. The approach of this paper is to presents a scheme that integrates information from most of the hyperspectral narrow-bands in order to increase the discrimination accuracy in unsupervised classification.

  5. Hyperspectral all-sky imaging of auroras.

    PubMed

    Sigernes, Fred; Ivanov, Yuriy; Chernouss, Sergey; Trondsen, Trond; Roldugin, Alexey; Fedorenko, Yury; Kozelov, Boris; Kirillov, Andrey; Kornilov, Ilia; Safargaleev, Vladimir; Holmen, Silje; Dyrland, Margit; Lorentzen, Dag; Baddeley, Lisa

    2012-12-01

    A prototype auroral hyperspectral all-sky camera has been constructed and tested. It uses electro-optical tunable filters to image the night sky as a function of wavelength throughout the visible spectrum with no moving mechanical parts. The core optical system includes a new high power all-sky lens with F-number equal to f/1.1. The camera has been tested at the Kjell Henriksen Observatory (KHO) during the auroral season of 2011/2012. It detects all sub classes of aurora above ~½ of the sub visual 1kR green intensity threshold at an exposure time of only one second. Supervised classification of the hyperspectral data shows promise as a new method to process and identify auroral forms. PMID:23262713

  6. Onboard Image Processing System for Hyperspectral Sensor.

    PubMed

    Hihara, Hiroki; Moritani, Kotaro; Inoue, Masao; Hoshi, Yoshihiro; Iwasaki, Akira; Takada, Jun; Inada, Hitomi; Suzuki, Makoto; Seki, Taeko; Ichikawa, Satoshi; Tanii, Jun

    2015-01-01

    Onboard image processing systems for a hyperspectral sensor have been developed in order to maximize image data transmission efficiency for large volume and high speed data downlink capacity. Since more than 100 channels are required for hyperspectral sensors on Earth observation satellites, fast and small-footprint lossless image compression capability is essential for reducing the size and weight of a sensor system. A fast lossless image compression algorithm has been developed, and is implemented in the onboard correction circuitry of sensitivity and linearity of Complementary Metal Oxide Semiconductor (CMOS) sensors in order to maximize the compression ratio. The employed image compression method is based on Fast, Efficient, Lossless Image compression System (FELICS), which is a hierarchical predictive coding method with resolution scaling. To improve FELICS's performance of image decorrelation and entropy coding, we apply a two-dimensional interpolation prediction and adaptive Golomb-Rice coding. It supports progressive decompression using resolution scaling while still maintaining superior performance measured as speed and complexity. Coding efficiency and compression speed enlarge the effective capacity of signal transmission channels, which lead to reducing onboard hardware by multiplexing sensor signals into a reduced number of compression circuits. The circuitry is embedded into the data formatter of the sensor system without adding size, weight, power consumption, and fabrication cost. PMID:26404281

  7. Onboard Image Processing System for Hyperspectral Sensor

    PubMed Central

    Hihara, Hiroki; Moritani, Kotaro; Inoue, Masao; Hoshi, Yoshihiro; Iwasaki, Akira; Takada, Jun; Inada, Hitomi; Suzuki, Makoto; Seki, Taeko; Ichikawa, Satoshi; Tanii, Jun

    2015-01-01

    Onboard image processing systems for a hyperspectral sensor have been developed in order to maximize image data transmission efficiency for large volume and high speed data downlink capacity. Since more than 100 channels are required for hyperspectral sensors on Earth observation satellites, fast and small-footprint lossless image compression capability is essential for reducing the size and weight of a sensor system. A fast lossless image compression algorithm has been developed, and is implemented in the onboard correction circuitry of sensitivity and linearity of Complementary Metal Oxide Semiconductor (CMOS) sensors in order to maximize the compression ratio. The employed image compression method is based on Fast, Efficient, Lossless Image compression System (FELICS), which is a hierarchical predictive coding method with resolution scaling. To improve FELICS’s performance of image decorrelation and entropy coding, we apply a two-dimensional interpolation prediction and adaptive Golomb-Rice coding. It supports progressive decompression using resolution scaling while still maintaining superior performance measured as speed and complexity. Coding efficiency and compression speed enlarge the effective capacity of signal transmission channels, which lead to reducing onboard hardware by multiplexing sensor signals into a reduced number of compression circuits. The circuitry is embedded into the data formatter of the sensor system without adding size, weight, power consumption, and fabrication cost. PMID:26404281

  8. Instrumental error in chromotomosynthetic hyperspectral imaging.

    PubMed

    Bostick, Randall L; Perram, Glen P

    2012-07-20

    Chromotomosynthetic imaging (CTI) is a method of convolving spatial and spectral information that can be reconstructed into a hyperspectral image cube using the same transforms employed in medical tomosynthesis. A direct vision prism instrument operating in the visible (400-725 nm) with 0.6 mrad instantaneous field of view (IFOV) and 0.6-10 nm spectral resolution has been constructed and characterized. Reconstruction of hyperspectral data cubes requires an estimation of the instrument component properties that define the forward transform. We analyze the systematic instrumental error in collected projection data resulting from prism spectral dispersion, prism alignment, detector array position, and prism rotation angle. The shifting and broadening of both the spectral lineshape function and the spatial point spread function in the reconstructed hyperspectral imagery is compared with experimental results for monochromatic point sources. The shorter wavelength (λ<500 nm) region where the prism has the highest spectral dispersion suffers mostly from degradation of spectral resolution in the presence of systematic error, while longer wavelengths (λ>600 nm) suffer mostly from a shift of the spectral peaks. The quality of the reconstructed hyperspectral imagery is most sensitive to the misalignment of the prism rotation mount. With less than 1° total angular error in the two axes of freedom, spectral resolution was degraded by as much as a factor of 2 in the blue spectral region. For larger errors than this, spectral peaks begin to split into bimodal distributions, and spatial point response functions are reconstructed in rings with radii proportional to wavelength and spatial resolution. PMID:22858961

  9. Hyperspectral confocal fluorescence imaging of cells

    NASA Astrophysics Data System (ADS)

    Haaland, David M.; Jones, Howland D. T.; Sinclair, Michael B.; Carson, Bryan; Branda, Catherine; Poschet, Jens F.; Rebeil, Roberto; Tian, Bing; Liu, Ping; Brasier, Allan R.

    2007-09-01

    Confocal fluorescence imaging of biological systems is an important method by which researchers can investigate molecular processes occurring in live cells. We have developed a new 3D hyperspectral confocal fluorescence microscope that can further enhance the usefulness of fluorescence microscopy in studying biological systems. The new microscope can increase the information content obtained from the image since, at each voxel, the microscope records 512 wavelengths from the emission spectrum (490 to 800 nm) while providing optical sectioning of samples with diffraction-limited spatial resolution. When coupled with multivariate curve resolution (MCR) analyses, the microscope can resolve multiple spatially and spectrally overlapped emission components, thereby greatly increasing the number of fluorescent labels, relative to most commercial microscopes, that can be monitored simultaneously. The MCR algorithm allows the "discovery" of all emitting sources and estimation of their relative concentrations without cross talk, including those emission sources that might not have been expected in the imaged cells. In this work, we have used the new microscope to obtain time-resolved hyperspectral images of cellular processes. We have quantitatively monitored the translocation of the GFP-labeled RelA protein (without interference from autofluorescence) into and out of the nucleus of live HeLa cells in response to continuous stimulation by the cytokine, TNFα. These studies have been extended to imaging live mouse macrophage cells with YFP-labeled RelA and GFP-labeled IRF3 protein. Hyperspectral imaging coupled with MCR analysis makes possible, for the first time, quantitative analysis of GFP, YFP, and autofluorescence without concern for cross-talk between emission sources. The significant power and quantitative capabilities of the new hyperspectral imaging system are further demonstrated with the imaging of a simple fluorescence dye (SYTO 13) traditionally used to stain the

  10. Pork grade evaluation using hyperspectral imaging techniques

    NASA Astrophysics Data System (ADS)

    Zhou, Rui; Cai, Bo; Wang, Shoubing; Ji, Huihua; Chen, Huacai

    2011-11-01

    The method to evaluate the grade of the pork based on hyperspectral imaging techniques was studied. Principal component analysis (PCA) was performed on the hyperspectral image data to extract the principal components which were used as the inputs of the evaluation model. By comparing the different discriminating rates in the calibration set and the validation set under different information, the choice of the components can be optimized. Experimental results showed that the classification evaluation model was the optimal when the principal of component (PC) of spectra was 3, while the corresponding discriminating rate was 89.1% in the calibration set and 84.9% in the validation set. It was also good when the PC of images was 9, while the corresponding discriminating rate was 97.2% in the calibration set and 91.1% in the validation set. The evaluation model based on both information of spectra and images was built, in which the corresponding PCs of spectra and images were used as the inputs. This model performed very well in grade classification evaluation, and the discriminating rates of calibration set and validation set were 99.5% and 92.7%, respectively, which were better than the two evaluation models based on single information of spectra or images.

  11. Hyperspectral and multispectral imaging for evaluating food safety and quality

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Spectral imaging technologies have been developed rapidly during the past decade. This paper presents hyperspectral and multispectral imaging technologies in the area of food safety and quality evaluation, with an introduction, demonstration, and summarization of the spectral imaging techniques avai...

  12. Modified wavelet kernel methods for hyperspectral image classification

    NASA Astrophysics Data System (ADS)

    Hsu, Pai-Hui; Huang, Xiu-Man

    2015-10-01

    Hyperspectral images have the capability of acquiring images of earth surface with several hundred of spectral bands. Providing such abundant spectral data should increase the abilities in classifying land use/cover type. However, due to the high dimensionality of hyperspectral data, traditional classification methods are not suitable for hyperspectral data classification. The common method to solve this problem is dimensionality reduction by using feature extraction before classification. Kernel methods such as support vector machine (SVM) and multiple kernel learning (MKL) have been successfully applied to hyperspectral images classification. In kernel methods applications, the selection of kernel function plays an important role. The wavelet kernel with multidimensional wavelet functions can find the optimal approximation of data in feature space for classification. The SVM with wavelet kernels (called WSVM) have been also applied to hyperspectral data and improve classification accuracy. In this study, wavelet kernel method combined multiple kernel learning algorithm and wavelet kernels was proposed for hyperspectral image classification. After the appropriate selection of a linear combination of kernel functions, the hyperspectral data will be transformed to the wavelet feature space, which should have the optimal data distribution for kernel learning and classification. Finally, the proposed methods were compared with the existing methods. A real hyperspectral data set was used to analyze the performance of wavelet kernel method. According to the results the proposed wavelet kernel methods in this study have well performance, and would be an appropriate tool for hyperspectral image classification.

  13. Ore minerals textural characterization by hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Bonifazi, Giuseppe; Picone, Nicoletta; Serranti, Silvia

    2013-02-01

    The utilization of hyperspectral detection devices, for natural resources mapping/exploitation through remote sensing techniques, dates back to the early 1970s. From the first devices utilizing a one-dimensional profile spectrometer, HyperSpectral Imaging (HSI) devices have been developed. Thus, from specific-customized devices, originally developed by Governmental Agencies (e.g. NASA, specialized research labs, etc.), a lot of HSI based equipment are today available at commercial level. Parallel to this huge increase of hyperspectral systems development/manufacturing, addressed to airborne application, a strong increase also occurred in developing HSI based devices for "ground" utilization that is sensing units able to play inside a laboratory, a processing plant and/or in an open field. Thanks to this diffusion more and more applications have been developed and tested in this last years also in the materials sectors. Such an approach, when successful, is quite challenging being usually reliable, robust and characterised by lower costs if compared with those usually associated to commonly applied analytical off- and/or on-line analytical approaches. In this paper such an approach is presented with reference to ore minerals characterization. According to the different phases and stages of ore minerals and products characterization, and starting from the analyses of the detected hyperspectral firms, it is possible to derive useful information about mineral flow stream properties and their physical-chemical attributes. This last aspect can be utilized to define innovative process mineralogy strategies and to implement on-line procedures at processing level. The present study discusses the effects related to the adoption of different hardware configurations, the utilization of different logics to perform the analysis and the selection of different algorithms according to the different characterization, inspection and quality control actions to apply.

  14. A Hyperspectral Imaging System for Quality Detection of Pickles

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral imaging system in simultaneous reflectance (400-675 nm) and transmittance (675-1000 nm) modes was developed for detection of hollow or bloater damage on whole pickles. Hyperspectral reflectance and transmittance images were acquired from normal and bloated whole pickle samples collec...

  15. Citrus greening detection using airborne hyperspectral and multispectral imaging techniques

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral imaging can provide unique spectral signatures for diseased vegetation. Airborne multispectral and hyperspectral imaging can be used to detect potentially infected trees over a large area for rapid detection of infected zones. This paper proposes a method to detect the citrus greening...

  16. Hyperspectral Imaging for Defect Detection of Pickling Cucumber

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This book chapter reviews the recent progress on hyperspectral imaging technology for defect inspection of pickling cucumbers. The chapter first describes near-infrared hyperspectral reflectance imaging technique for the detection of bruises on pickling cucumbers. The technique showed good detection...

  17. Citrus greening disease detection using airborne multispectral and hyperspectral imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral imaging can provide unique spectral signatures for diseased vegetation. Airborne hyperspectral imaging can be used to detect potentially infected trees over a large area for rapid detection of infected zones. Ground inspection and management can be focused on these infected zones rath...

  18. Detection of hatching and table egg defects using hyperspectral imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral imaging system was developed to detect problem hatching eggs (non-fertile or dead embryos) prior to or during early incubation and to detect table eggs with blood spots and cracked shells. All eggs were imaged using a hyperspectral camera system (wavelengths detected from 400-900mm) ...

  19. Online Unmixing of Multitemporal Hyperspectral Images Accounting for Spectral Variability

    NASA Astrophysics Data System (ADS)

    Thouvenin, Pierre-Antoine; Dobigeon, Nicolas; Tourneret, Jean-Yves

    2016-09-01

    Hyperspectral unmixing is aimed at identifying the reference spectral signatures composing an hyperspectral image and their relative abundance fractions in each pixel. In practice, the identified signatures may vary spectrally from an image to another due to varying acquisition conditions, thus inducing possibly significant estimation errors. Against this background, hyperspectral unmixing of several images acquired over the same area is of considerable interest. Indeed, such an analysis enables the endmembers of the scene to be tracked and the corresponding endmember variability to be characterized. Sequential endmember estimation from a set of hyperspectral images is expected to provide improved performance when compared to methods analyzing the images independently. However, the significant size of hyperspectral data precludes the use of batch procedures to jointly estimate the mixture parameters of a sequence of hyperspectral images. Provided that each elementary component is present in at least one image of the sequence, we propose to perform an online hyperspectral unmixing accounting for temporal endmember variability. The online hyperspectral unmixing is formulated as a two-stage stochastic program, which can be solved using a stochastic approximation. The performance of the proposed method is evaluated on synthetic and real data. A comparison with independent unmixing algorithms finally illustrates the interest of the proposed strategy.

  20. Online Unmixing of Multitemporal Hyperspectral Images Accounting for Spectral Variability.

    PubMed

    Thouvenin, Pierre-Antoine; Dobigeon, Nicolas; Tourneret, Jean-Yves

    2016-09-01

    Hyperspectral unmixing is aimed at identifying the reference spectral signatures composing a hyperspectral image and their relative abundance fractions in each pixel. In practice, the identified signatures may vary spectrally from an image to another due to varying acquisition conditions, thus inducing possibly significant estimation errors. Against this background, the hyperspectral unmixing of several images acquired over the same area is of considerable interest. Indeed, such an analysis enables the endmembers of the scene to be tracked and the corresponding endmember variability to be characterized. Sequential endmember estimation from a set of hyperspectral images is expected to provide improved performance when compared with methods analyzing the images independently. However, the significant size of the hyperspectral data precludes the use of batch procedures to jointly estimate the mixture parameters of a sequence of hyperspectral images. Provided that each elementary component is present in at least one image of the sequence, we propose to perform an online hyperspectral unmixing accounting for temporal endmember variability. The online hyperspectral unmixing is formulated as a two-stage stochastic program, which can be solved using a stochastic approximation. The performance of the proposed method is evaluated on synthetic and real data. Finally, a comparison with independent unmixing algorithms illustrates the interest of the proposed strategy. PMID:27305679

  1. On-orbit characterization of hyperspectral imagers

    NASA Astrophysics Data System (ADS)

    McCorkel, Joel

    Remote Sensing Group (RSG) at the University of Arizona has a long history of using ground-based test sites for the calibration of airborne- and satellite-based sensors. Often, ground-truth measurements at these tests sites are not always successful due to weather and funding availability. Therefore, RSG has also employed automated ground instrument approaches and cross-calibration methods to verify the radiometric calibration of a sensor. The goal in the cross-calibration method is to transfer the calibration of a well-known sensor to that of a different sensor. This dissertation presents a method for determining the radiometric calibration of a hyperspectral imager using multispectral imagery. The work relies on a multispectral sensor, Moderate-resolution Imaging Spectroradiometer (MODIS), as a reference for the hyperspectral sensor Hyperion. Test sites used for comparisons are Railroad Valley in Nevada and a portion of the Libyan Desert in North Africa. A method to predict hyperspectral surface reflectance using a combination of MODIS data and spectral shape information is developed and applied for the characterization of Hyperion. Spectral shape information is based on RSG's historical in situ data for the Railroad Valley test site and spectral library data for the Libyan test site. Average atmospheric parameters, also based on historical measurements, are used in reflectance prediction and transfer to space. Results of several cross-calibration scenarios that differ in image acquisition coincidence, test site, and reference sensor are found for the characterization of Hyperion. These are compared with results from the reflectance-based approach of vicarious calibration, a well-documented method developed by the RSG that serves as a baseline for calibration performance for the cross-calibration method developed here. Cross-calibration provides results that are within 2% of those of reflectance-based results in most spectral regions. Larger disagreements exist

  2. Reconfigurable Hardware for Compressing Hyperspectral Image Data

    NASA Technical Reports Server (NTRS)

    Aranki, Nazeeh; Namkung, Jeffrey; Villapando, Carlos; Kiely, Aaron; Klimesh, Matthew; Xie, Hua

    2010-01-01

    High-speed, low-power, reconfigurable electronic hardware has been developed to implement ICER-3D, an algorithm for compressing hyperspectral-image data. The algorithm and parts thereof have been the topics of several NASA Tech Briefs articles, including Context Modeler for Wavelet Compression of Hyperspectral Images (NPO-43239) and ICER-3D Hyperspectral Image Compression Software (NPO-43238), which appear elsewhere in this issue of NASA Tech Briefs. As described in more detail in those articles, the algorithm includes three main subalgorithms: one for computing wavelet transforms, one for context modeling, and one for entropy encoding. For the purpose of designing the hardware, these subalgorithms are treated as modules to be implemented efficiently in field-programmable gate arrays (FPGAs). The design takes advantage of industry- standard, commercially available FPGAs. The implementation targets the Xilinx Virtex II pro architecture, which has embedded PowerPC processor cores with flexible on-chip bus architecture. It incorporates an efficient parallel and pipelined architecture to compress the three-dimensional image data. The design provides for internal buffering to minimize intensive input/output operations while making efficient use of offchip memory. The design is scalable in that the subalgorithms are implemented as independent hardware modules that can be combined in parallel to increase throughput. The on-chip processor manages the overall operation of the compression system, including execution of the top-level control functions as well as scheduling, initiating, and monitoring processes. The design prototype has been demonstrated to be capable of compressing hyperspectral data at a rate of 4.5 megasamples per second at a conservative clock frequency of 50 MHz, with a potential for substantially greater throughput at a higher clock frequency. The power consumption of the prototype is less than 6.5 W. The reconfigurability (by means of reprogramming) of

  3. Research on a project of the new computational hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Li, Huan; Zhou, Feng; Zhang, Zhi; Shi, Guang-ming

    2012-09-01

    This paper brings hyperspectral technology and compute image together, on the basis of geometrical optics theory and compressed sensing theory, put forward a new computational spectral Imaging technology. That raises two to four times on spatial resolution and double on spectral resolution compared conventional hyperspectral imagers. Owing to have finished compressing when getting the imaging signal, that could resolve the conflict between the mass of data bringing with high resolution and transfers and storage. The paper carries out a project to the new hyperspectral imager.

  4. Compressive Hyperspectral Imaging via Approximate Message Passing

    NASA Astrophysics Data System (ADS)

    Tan, Jin; Ma, Yanting; Rueda, Hoover; Baron, Dror; Arce, Gonzalo R.

    2016-03-01

    We consider a compressive hyperspectral imaging reconstruction problem, where three-dimensional spatio-spectral information about a scene is sensed by a coded aperture snapshot spectral imager (CASSI). The CASSI imaging process can be modeled as suppressing three-dimensional coded and shifted voxels and projecting these onto a two-dimensional plane, such that the number of acquired measurements is greatly reduced. On the other hand, because the measurements are highly compressive, the reconstruction process becomes challenging. We previously proposed a compressive imaging reconstruction algorithm that is applied to two-dimensional images based on the approximate message passing (AMP) framework. AMP is an iterative algorithm that can be used in signal and image reconstruction by performing denoising at each iteration. We employed an adaptive Wiener filter as the image denoiser, and called our algorithm "AMP-Wiener." In this paper, we extend AMP-Wiener to three-dimensional hyperspectral image reconstruction, and call it "AMP-3D-Wiener." Applying the AMP framework to the CASSI system is challenging, because the matrix that models the CASSI system is highly sparse, and such a matrix is not suitable to AMP and makes it difficult for AMP to converge. Therefore, we modify the adaptive Wiener filter and employ a technique called damping to solve for the divergence issue of AMP. Our approach is applied in nature, and the numerical experiments show that AMP-3D-Wiener outperforms existing widely-used algorithms such as gradient projection for sparse reconstruction (GPSR) and two-step iterative shrinkage/thresholding (TwIST) given a similar amount of runtime. Moreover, in contrast to GPSR and TwIST, AMP-3D-Wiener need not tune any parameters, which simplifies the reconstruction process.

  5. Hyperspectral imaging applied to forensic medicine

    NASA Astrophysics Data System (ADS)

    Malkoff, Donald B.; Oliver, William R.

    2000-03-01

    Remote sensing techniques now include the use of hyperspectral infrared imaging sensors covering the mid-and- long wave regions of the spectrum. They have found use in military surveillance applications due to their capability for detection and classification of a large variety of both naturally occurring and man-made substances. The images they produce reveal the spatial distributions of spectral patterns that reflect differences in material temperature, texture, and composition. A program is proposed for demonstrating proof-of-concept in using a portable sensor of this type for crime scene investigations. It is anticipated to be useful in discovering and documenting the affects of trauma and/or naturally occurring illnesses, as well as detecting blood spills, tire patterns, toxic chemicals, skin injection sites, blunt traumas to the body, fluid accumulations, congenital biochemical defects, and a host of other conditions and diseases. This approach can significantly enhance capabilities for determining the circumstances of death. Potential users include law enforcement organizations (police, FBI, CIA), medical examiners, hospitals/emergency rooms, and medical laboratories. Many of the image analysis algorithms already in place for hyperspectral remote sensing and crime scene investigations can be applied to the interpretation of data obtained in this program.

  6. Hyperspectral imaging from space: Warfighter-1

    NASA Astrophysics Data System (ADS)

    Cooley, Thomas; Seigel, Gary; Thorsos, Ivan

    1999-01-01

    The Air Force Research Laboratory Integrated Space Technology Demonstrations (ISTD) Program Office has partnered with Orbital Sciences Corporation (OSC) to complement the commercial satellite's high-resolution panchromatic imaging and Multispectral imaging (MSI) systems with a moderate resolution Hyperspectral imaging (HSI) spectrometer camera. The program is an advanced technology demonstration utilizing a commercially based space capability to provide unique functionality in remote sensing technology. This leveraging of commercial industry to enhance the value of the Warfighter-1 program utilizes the precepts of acquisition reform and is a significant departure from the old-school method of contracting for government managed large demonstration satellites with long development times and technology obsolescence concerns. The HSI system will be able to detect targets from the spectral signature measured by the hyperspectral camera. The Warfighter-1 program will also demonstrate the utility of the spectral information to theater military commanders and intelligence analysts by transmitting HSI data directly to a mobile ground station that receives and processes the data. After a brief history of the project origins, this paper will present the details of the Warfighter-1 system and expected results from exploitation of HSI data as well as the benefits realized by this collaboration between the Air Force and commercial industry.

  7. Dental caries imaging using hyperspectral stimulated Raman scattering microscopy

    NASA Astrophysics Data System (ADS)

    Wang, Zi; Zheng, Wei; Jian, Lin; Huang, Zhiwei

    2016-03-01

    We report the development of a polarization-resolved hyperspectral stimulated Raman scattering (SRS) imaging technique based on a picosecond (ps) laser-pumped optical parametric oscillator system for label-free imaging of dental caries. In our imaging system, hyperspectral SRS images (512×512 pixels) in both fingerprint region (800-1800 cm-1) and high-wavenumber region (2800-3600 cm-1) are acquired in minutes by scanning the wavelength of OPO output, which is a thousand times faster than conventional confocal micro Raman imaging. SRS spectra variations from normal enamel to caries obtained from the hyperspectral SRS images show the loss of phosphate and carbonate in the carious region. While polarization-resolved SRS images at 959 cm-1 demonstrate that the caries has higher depolarization ratio. Our results demonstrate that the polarization resolved-hyperspectral SRS imaging technique developed allows for rapid identification of the biochemical and structural changes of dental caries.

  8. Optical design of wide swath hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Wang, Yueming; Yuan, Liyin; Wang, Jianyu

    2014-05-01

    This paper describes a design concept for wide swath hyperspectral imager. The challenge is to meet the requirement of good image quality and high precision registration from 400nm to 2500nm. A new type spherical prism imaging spectrometer is presented in the paper. The swath of system can reach 60 kilometer from a 600km sun-synchronous orbit with 30 meter ground sample distance (GSD). The optical system consists of a TMA objective and 2 30mm-slit spherical prism spectrometer operating both VNIR and SWIR. Key features of the design include (1) high signal to noise ratio for high efficiency of F-silica prism; (2) high precision band registration for same spectrometer operating from 400nm to 2500nm.

  9. Hyperspectral imaging for melanoma screening

    NASA Astrophysics Data System (ADS)

    Martin, Justin; Krueger, James; Gareau, Daniel

    2014-03-01

    The 5-year survival rate for patients diagnosed with Melanoma, a deadly form of skin cancer, in its latest stages is about 15%, compared to over 90% for early detection and treatment. We present an imaging system and algorithm that can be used to automatically generate a melanoma risk score to aid clinicians in the early identification of this form of skin cancer. Our system images the patient's skin at a series of different wavelengths and then analyzes several key dermoscopic features to generate this risk score. We have found that shorter wavelengths of light are sensitive to information in the superficial areas of the skin while longer wavelengths can be used to gather information at greater depths. This accompanying diagnostic computer algorithm has demonstrated much higher sensitivity and specificity than the currently commercialized system in preliminary trials and has the potential to improve the early detection of melanoma.

  10. Mapping pigment distribution in mud samples through hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Mehrübeoglu, Mehrube; Nicula, Cosmina; Trombley, Christopher; Smith, Shane W.; Smith, Dustin K.; Shanks, Elizabeth S.; Zimba, Paul V.

    2015-09-01

    Mud samples collected from bodies of water reveal information about the distribution of microorganisms in the local sediments. Hyperspectral imaging has been investigated as a technology to identify phototropic organisms living on sediments collected from the Texas Coastal Bend area based on their spectral pigment profiles and spatial arrangement. The top pigment profiles identified through high-performance liquid chromatography (HPLC) have been correlated with spectral signatures extracted from the hyperspectral data of mud using fast Fourier transform (FFT). Spatial distributions have also been investigated using 2D hyperspectral image processing. 2D pigment distribution maps have been created based on the correlation with pigment profiles in the FFT domain. Among the tested pigments, the results show match among four out of five pigment distribution trends between HPLC and hyperspectral data analysis. Differences are attributed mainly to the difference between area and volume of scale between the HPLC analysis and area covered by hyperspectral imaging.

  11. Image visualization of hyperspectral spectrum for LWIR

    NASA Astrophysics Data System (ADS)

    Chong, Eugene; Jeong, Young-Su; Lee, Jai-Hoon; Park, Dong Jo; Kim, Ju Hyun

    2015-07-01

    The image visualization of a real-time hyperspectral spectrum in the long-wave infrared (LWIR) range of 900-1450 cm-1 by a color-matching function is addressed. It is well known that the absorption spectra of main toxic industrial chemical (TIC) and chemical warfare agent (CWA) clouds are detected in this spectral region. Furthermore, a significant spectral peak due to various background species and unknown targets are also present. However, those are dismissed as noise, resulting in utilization limit. Herein, we applied a color-matching function that uses the information from hyperspectral data, which is emitted from the materials and surfaces of artificial or natural backgrounds in the LWIR region. This information was used to classify and differentiate the background signals from the targeted substances, and the results were visualized as image data without additional visual equipment. The tristimulus value based visualization information can quickly identify the background species and target in real-time detection in LWIR.

  12. Dried fruits quality assessment by hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Serranti, Silvia; Gargiulo, Aldo; Bonifazi, Giuseppe

    2012-05-01

    Dried fruits products present different market values according to their quality. Such a quality is usually quantified in terms of freshness of the products, as well as presence of contaminants (pieces of shell, husk, and small stones), defects, mould and decays. The combination of these parameters, in terms of relative presence, represent a fundamental set of attributes conditioning dried fruits humans-senses-detectable-attributes (visual appearance, organolectic properties, etc.) and their overall quality in terms of marketable products. Sorting-selection strategies exist but sometimes they fail when a higher degree of detection is required especially if addressed to discriminate between dried fruits of relatively small dimensions and when aiming to perform an "early detection" of pathogen agents responsible of future moulds and decays development. Surface characteristics of dried fruits can be investigated by hyperspectral imaging (HSI). In this paper, specific and "ad hoc" applications addressed to propose quality detection logics, adopting a hyperspectral imaging (HSI) based approach, are described, compared and critically evaluated. Reflectance spectra of selected dried fruits (hazelnuts) of different quality and characterized by the presence of different contaminants and defects have been acquired by a laboratory device equipped with two HSI systems working in two different spectral ranges: visible-near infrared field (400-1000 nm) and near infrared field (1000-1700 nm). The spectra have been processed and results evaluated adopting both a simple and fast wavelength band ratio approach and a more sophisticated classification logic based on principal component (PCA) analysis.

  13. Hyperspectral Fluorescence and Reflectance Imaging Instrument

    NASA Technical Reports Server (NTRS)

    Ryan, Robert E.; O'Neal, S. Duane; Lanoue, Mark; Russell, Jeffrey

    2008-01-01

    The system is a single hyperspectral imaging instrument that has the unique capability to acquire both fluorescence and reflectance high-spatial-resolution data that is inherently spatially and spectrally registered. Potential uses of this instrument include plant stress monitoring, counterfeit document detection, biomedical imaging, forensic imaging, and general materials identification. Until now, reflectance and fluorescence spectral imaging have been performed by separate instruments. Neither a reflectance spectral image nor a fluorescence spectral image alone yields as much information about a target surface as does a combination of the two modalities. Before this system was developed, to benefit from this combination, analysts needed to perform time-consuming post-processing efforts to co-register the reflective and fluorescence information. With this instrument, the inherent spatial and spectral registration of the reflectance and fluorescence images minimizes the need for this post-processing step. The main challenge for this technology is to detect the fluorescence signal in the presence of a much stronger reflectance signal. To meet this challenge, the instrument modulates artificial light sources from ultraviolet through the visible to the near-infrared part of the spectrum; in this way, both the reflective and fluorescence signals can be measured through differencing processes to optimize fluorescence and reflectance spectra as needed. The main functional components of the instrument are a hyperspectral imager, an illumination system, and an image-plane scanner. The hyperspectral imager is a one-dimensional (line) imaging spectrometer that includes a spectrally dispersive element and a two-dimensional focal plane detector array. The spectral range of the current imaging spectrometer is between 400 to 1,000 nm, and the wavelength resolution is approximately 3 nm. The illumination system consists of narrowband blue, ultraviolet, and other discrete

  14. Hyperspectral Image Turbulence Measurements of the Atmosphere

    NASA Technical Reports Server (NTRS)

    Lane, Sarah E.; West, Leanne L.; Gimmestad, Gary G.; Kireev, Stanislav; Smith, William L., Sr.; Burdette, Edward M.; Daniels, Taumi; Cornman, Larry

    2012-01-01

    A Forward Looking Interferometer (FLI) sensor has the potential to be used as a means of detecting aviation hazards in flight. One of these hazards is mountain wave turbulence. The results from a data acquisition activity at the University of Colorado s Mountain Research Station will be presented here. Hyperspectral datacubes from a Telops Hyper-Cam are being studied to determine if evidence of a turbulent event can be identified in the data. These data are then being compared with D&P TurboFT data, which are collected at a much higher time resolution and broader spectrum.

  15. High spectral resolution airborne short wave infrared hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Wei, Liqing; Yuan, Liyin; Wang, Yueming; Zhuang, Xiaoqiong

    2016-05-01

    Short Wave InfraRed(SWIR) spectral imager is good at detecting difference between materials and penetrating fog and mist. High spectral resolution SWIR hyperspectral imager plays a key role in developing earth observing technology. Hyperspectral data cube can help band selections that is very important for multispectral imager design. Up to now, the spectral resolution of many SWIR hyperspectral imagers is about 10nm. A high sensitivity airborne SWIR hyperspectral imager with narrower spectral band will be presented. The system consists of TMA telescope, slit, spectrometer with planar blazed grating and high sensitivity MCT FPA. The spectral sampling interval is about 3nm. The IFOV is 0.5mrad. To eliminate the influence of the thermal background, a cold shield is designed in the dewar. The pixel number of spatial dimension is 640. Performance measurement in laboratory and image analysis for flight test will also be presented.

  16. System and method for progressive band selection for hyperspectral images

    NASA Technical Reports Server (NTRS)

    Fisher, Kevin (Inventor)

    2013-01-01

    Disclosed herein are systems, methods, and non-transitory computer-readable storage media for progressive band selection for hyperspectral images. A system having module configured to control a processor to practice the method calculates a virtual dimensionality of a hyperspectral image having multiple bands to determine a quantity Q of how many bands are needed for a threshold level of information, ranks each band based on a statistical measure, selects Q bands from the multiple bands to generate a subset of bands based on the virtual dimensionality, and generates a reduced image based on the subset of bands. This approach can create reduced datasets of full hyperspectral images tailored for individual applications. The system uses a metric specific to a target application to rank the image bands, and then selects the most useful bands. The number of bands selected can be specified manually or calculated from the hyperspectral image's virtual dimensionality.

  17. Metric Learning for Hyperspectral Image Segmentation

    NASA Technical Reports Server (NTRS)

    Bue, Brian D.; Thompson, David R.; Gilmore, Martha S.; Castano, Rebecca

    2011-01-01

    We present a metric learning approach to improve the performance of unsupervised hyperspectral image segmentation. Unsupervised spatial segmentation can assist both user visualization and automatic recognition of surface features. Analysts can use spatially-continuous segments to decrease noise levels and/or localize feature boundaries. However, existing segmentation methods use tasks-agnostic measures of similarity. Here we learn task-specific similarity measures from training data, improving segment fidelity to classes of interest. Multiclass Linear Discriminate Analysis produces a linear transform that optimally separates a labeled set of training classes. The defines a distance metric that generalized to a new scenes, enabling graph-based segmentation that emphasizes key spectral features. We describe tests based on data from the Compact Reconnaissance Imaging Spectrometer (CRISM) in which learned metrics improve segment homogeneity with respect to mineralogical classes.

  18. Does virtual dimensionality work in hyperspectral images?

    NASA Astrophysics Data System (ADS)

    Bajorski, Peter

    2009-05-01

    The effective dimensionality (ED) of hyperspectral images is often viewed as the dimensionality of an affine subspace defined by linear combinations of spectra of materials present in the image. That affine subspace is expected to give an acceptable approximation to all pixels. At this point, there is no precise definition of ED. In an effort to assess ED, a notion of virtual dimensionality (VD) has been developed, and it is being used in many papers including those published in TGARS. The ever- spreading use of VD warrants its thorough investigation. In this paper, we investigate properties of VD, and we show that VD largely depends on the average value of all spectra rather than on ED. We show specific examples when VD would give entirely misleading results. We also explain fallacies associated with justifications for VD.

  19. Hyperspectral image reconstruction for diffuse optical tomography

    PubMed Central

    Larusson, Fridrik; Fantini, Sergio; Miller, Eric L.

    2011-01-01

    We explore the development and performance of algorithms for hyperspectral diffuse optical tomography (DOT) for which data from hundreds of wavelengths are collected and used to determine the concentration distribution of chromophores in the medium under investigation. An efficient method is detailed for forming the images using iterative algorithms applied to a linearized Born approximation model assuming the scattering coefficient is spatially constant and known. The L-surface framework is employed to select optimal regularization parameters for the inverse problem. We report image reconstructions using 126 wavelengths with estimation error in simulations as low as 0.05 and mean square error of experimental data of 0.18 and 0.29 for ink and dye concentrations, respectively, an improvement over reconstructions using fewer specifically chosen wavelengths. PMID:21483616

  20. Comparison of support vector machine-based processing chains for hyperspectral image classification

    NASA Astrophysics Data System (ADS)

    Rojas, Marta; Dópido, Inmaculada; Plaza, Antonio; Gamba, Paolo

    2010-08-01

    Many different approaches have been proposed in recent years for remotely sensed hyperspectral image classification. Despite the variety of techniques designed to tackle the aforementioned problem, the definition of standardized processing chains for hyperspectral image classification is a difficult objective, which may ultimately depend on the application being addressed. Generally speaking, a hyperspectral image classification chain may be defined from two perspectives: 1) the provider's viewpoint, and 2) the user's viewpoint, where the first part of the chain comprises activities such as data calibration and geo-correction aspects, while the second part of the chain comprises information extraction processes from the collected data. The modules in the second part of the chain (which constitutes our main focus in this paper) should be ideally flexible enough to be accommodated not only to different application scenarios, but also to different hyperspectral imaging instruments with varying characteristics, and spatial and spectral resolutions. In this paper, we evaluate the performance of different processing chains resulting from combinations of modules for dimensionality reduction, feature extraction/ selection, image classification, and spatial post-processing. The support vector machine (SVM) classifier is adopted as a baseline due to its ability to classify hyperspectral data sets using limited training samples. A specific classification scenario is investigated, using a reference hyperspectral data set collected by NASA's Airborne Visible Infra-Red Imaging Spectrometer (AVIRIS) over the Indian Pines region in Indiana, USA.

  1. Development of image mappers for hyperspectral biomedical imaging applications

    PubMed Central

    Kester, Robert T.; Gao, Liang; Tkaczyk, Tomasz S.

    2010-01-01

    A new design and fabrication method is presented for creating large-format (>100 mirror facets) image mappers for a snapshot hyperspectral biomedical imaging system called an image mapping spectrometer (IMS). To verify this approach a 250 facet image mapper with 25 multiple-tilt angles is designed for a compact IMS that groups the 25 subpupils in a 5 × 5 matrix residing within a single collecting objective's pupil. The image mapper is fabricated by precision diamond raster fly cutting using surface-shaped tools. The individual mirror facets have minimal edge eating, tilt errors of <1 mrad, and an average roughness of 5.4 nm. PMID:20357875

  2. Characterization of burns using hyperspectral imaging technique - a preliminary study.

    PubMed

    Calin, Mihaela Antonina; Parasca, Sorin Viorel; Savastru, Roxana; Manea, Dragos

    2015-02-01

    Surgical burn treatment depends on accurate estimation of burn depth. Many methods have been used to asses burns, but none has gained wide acceptance. Hyperspectral imaging technique has recently entered the medical research field with encouraging results. In this paper we present a preliminary study (case presentation) that aims to point out the value of this optical method in burn wound characterization and to set up future lines of investigation. A hyperspectral image of a leg and foot with partial thickness burns was obtained in the fifth postburn day. The image was analyzed using linear spectral unmixing model as a tool for mapping the investigated areas. The article gives details on the mathematical bases of the interpretation model and correlations with clinical examination pointing out the advantages of hyperspectral imaging technique. While the results were encouraging, further more extended and better founded studies are being prepared before recognizing hyperspectral imaging technique as an applicable method of burn wound assessment. PMID:24997530

  3. Food quality assessment by NIR hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Whitworth, Martin B.; Millar, Samuel J.; Chau, Astor

    2010-04-01

    Near infrared reflectance (NIR) spectroscopy is well established in the food industry for rapid compositional analysis of bulk samples. NIR hyperspectral imaging provides new opportunities to measure the spatial distribution of components such as moisture and fat, and to identify and measure specific regions of composite samples. An NIR hyperspectral imaging system has been constructed for food research applications, incorporating a SWIR camera with a cooled 14 bit HgCdTe detector and N25E spectrograph (Specim Ltd, Finland). Samples are scanned in a pushbroom mode using a motorised stage. The system has a spectral resolution of 256 pixels covering a range of 970-2500 nm and a spatial resolution of 320 pixels covering a swathe adjustable from 8 to 300 mm. Images are acquired at a rate of up to 100 lines s-1, enabling samples to be scanned within a few seconds. Data are captured using SpectralCube software (Specim) and analysed using ENVI and IDL (ITT Visual Information Solutions). Several food applications are presented. The strength of individual absorbance bands enables the distribution of particular components to be assessed. Examples are shown for detection of added gluten in wheat flour and to study the effect of processing conditions on fat distribution in chips/French fries. More detailed quantitative calibrations have been developed to study evolution of the moisture distribution in baguettes during storage at different humidities, to assess freshness of fish using measurements of whole cod and fillets, and for prediction of beef quality by identification and separate measurement of lean and fat regions.

  4. Hyperspectral image classifier based on beach spectral feature

    NASA Astrophysics Data System (ADS)

    Liang, Zhang; Lianru, Gao; Bing, Zhang

    2014-03-01

    The seashore, especially coral bank, is sensitive to human activities and environmental changes. A multispectral image, with coarse spectral resolution, is inadaptable for identify subtle spectral distinctions between various beaches. To the contrary, hyperspectral image with narrow and consecutive channels increases our capability to retrieve minor spectral features which is suit for identification and classification of surface materials on the shore. Herein, this paper used airborne hyperspectral data, in addition to ground spectral data to study the beaches in Qingdao. The image data first went through image pretreatment to deal with the disturbance of noise, radiation inconsistence and distortion. In succession, the reflection spectrum, the derivative spectrum and the spectral absorption features of the beach surface were inspected in search of diagnostic features. Hence, spectra indices specific for the unique environment of seashore were developed. According to expert decisions based on image spectrums, the beaches are ultimately classified into sand beach, rock beach, vegetation beach, mud beach, bare land and water. In situ surveying reflection spectrum from GER1500 field spectrometer validated the classification production. In conclusion, the classification approach under expert decision based on feature spectrum is proved to be feasible for beaches.

  5. Hyperspectral imaging for safety inspection of food and agricultural products

    NASA Astrophysics Data System (ADS)

    Lu, Renfu; Chen, Yud-Ren

    1999-01-01

    Development of effective food inspection systems is critical in successful implementation of the hazard analysis and critical control points (HACCP) program. Hyperspectral imaging or imaging spectroscopy, which combines techniques of imaging and spectroscopy to acquire spatial and spectral information simultaneously, has great potential in food quality and safety inspection. This paper reviewed the basic principle and features of hyperspectral imaging and its hardware and software implementation. The potential areas of application for hyperspectral imaging in food quality and safety inspection were identified and its limitations were discussed. A hyperspectral imaging system developed for research in food quality and safety inspection was described. Experiments were performed to acquire hyperspectral images from four classes of poultry carcasses: normal, cadaver, septicemia, and tumor. Noticeable differences in the spectra of the relative reflectance and its second difference in the wavelengths between 430 nm and 900 nm were observed between wholesome and unwholesome carcasses. Differences among the three classes of unwholesome carcasses were also observed from their respective spectra. These results showed that hyperspectral imaging can be an effective tool for safety inspection of poultry carcasses.

  6. Hyperspectral image compression using an online learning method

    NASA Astrophysics Data System (ADS)

    Ülkü, Ä.°rem; Töreyin, B. Uǧur

    2015-05-01

    A hyperspectral image compression method is proposed using an online dictionary learning approach. The online learning mechanism is aimed at utilizing least number of dictionary elements for each hyperspectral image under consideration. In order to meet this "sparsity constraint", basis pursuit algorithm is used. Hyperspectral imagery from AVIRIS datasets are used for testing purposes. Effects of non-zero dictionary elements on the compression performance are analyzed. Results indicate that, the proposed online dictionary learning algorithm may be utilized for higher data rates, as it performs better in terms of PSNR values, as compared with the state-of-the-art predictive lossy compression schemes.

  7. Unsupervised hyperspectral image analysis using independent component analysis (ICA)

    SciTech Connect

    S. S. Chiang; I. W. Ginsberg

    2000-06-30

    In this paper, an ICA-based approach is proposed for hyperspectral image analysis. It can be viewed as a random version of the commonly used linear spectral mixture analysis, in which the abundance fractions in a linear mixture model are considered to be unknown independent signal sources. It does not require the full rank of the separating matrix or orthogonality as most ICA methods do. More importantly, the learning algorithm is designed based on the independency of the material abundance vector rather than the independency of the separating matrix generally used to constrain the standard ICA. As a result, the designed learning algorithm is able to converge to non-orthogonal independent components. This is particularly useful in hyperspectral image analysis since many materials extracted from a hyperspectral image may have similar spectral signatures and may not be orthogonal. The AVIRIS experiments have demonstrated that the proposed ICA provides an effective unsupervised technique for hyperspectral image classification.

  8. Black Beauty's Rainbow: Hyperspectral Imaging of Northwest Africa 7034

    NASA Astrophysics Data System (ADS)

    Cannon, K. M.; Mustard, J. F.; Agee, C. B.; Wilson, J. H.; Greenberger, R. N.

    2014-07-01

    Hyperspectral imaging is used to characterize the first basaltic breccia from Mars, Northwest Africa 7034. Initial results show the spectral character of NWA 7034 is unlike other SNC meteorites and may be more representative of average martian crust.

  9. An evaluation of popular hyperspectral images classification approaches

    NASA Astrophysics Data System (ADS)

    Kuznetsov, Andrey; Myasnikov, Vladislav

    2015-12-01

    This work is devoted to the problem of the best hyperspectral images classification algorithm selection. The following algorithms are used for comparison: decision tree using full cross-validation; decision tree C 4.5; Bayesian classifier; maximum-likelihood method; MSE minimization classifier, including a special case - classification by conjugation; spectral angle classifier (for empirical mean and nearest neighbor), spectral mismatch classifier and support vector machine (SVM). There are used AVIRIS and SpecTIR hyperspectral images to conduct experiments.

  10. Infrared hyperspectral imaging sensor for gas detection

    NASA Astrophysics Data System (ADS)

    Hinnrichs, Michele

    2000-11-01

    A small light weight man portable imaging spectrometer has many applications; gas leak detection, flare analysis, threat warning, chemical agent detection, just to name a few. With support from the US Air Force and Navy, Pacific Advanced Technology has developed a small man portable hyperspectral imaging sensor with an embedded DSP processor for real time processing that is capable of remotely imaging various targets such as gas plums, flames and camouflaged targets. Based upon their spectral signature the species and concentration of gases can be determined. This system has been field tested at numerous places including White Mountain, CA, Edwards AFB, and Vandenberg AFB. Recently evaluation of the system for gas detection has been performed. This paper presents these results. The system uses a conventional infrared camera fitted with a diffractive optic that images as well as disperses the incident radiation to form spectral images that are collected in band sequential mode. Because the diffractive optic performs both imaging and spectral filtering, the lens system consists of only a single element that is small, light weight and robust, thus allowing man portability. The number of spectral bands are programmable such that only those bands of interest need to be collected. The system is entirely passive, therefore, easily used in a covert operation. Currently Pacific Advanced Technology is working on the next generation of this camera system that will have both an embedded processor as well as an embedded digital signal processor in a small hand held camera configuration. This will allow the implementation of signal and image processing algorithms for gas detection and identification in real time. This paper presents field test data on gas detection and identification as well as discuss the signal and image processing used to enhance the gas visibility. Flow rates as low as 0.01 cubic feet per minute have been imaged with this system.

  11. NIR hyperspectral imaging to evaluate degradation in captopril commercial tablets.

    PubMed

    França, Leandro de Moura; Pimentel, Maria Fernanda; Simões, Simone da Silva; Grangeiro, Severino; Prats-Montalbán, José M; Ferrer, Alberto

    2016-07-01

    Pharmaceutical quality control is important for improving the effectiveness, purity and safety of drugs, as well as for the prevention or control of drug degradation. In the present work, near infrared hyperspectral images (HSI-NIR) of tablets with different expiration dates were employed to evaluate the degradation of captopril into captopril disulfide in different layers, on the top and on the bottom surfaces of the tablets. Multivariate curve resolution (MCR) models were used to extract the concentration distribution maps from the hyperspectral images. Afterward, multivariate image techniques were applied to the concentration distribution maps (CDMs), to extract features and build models relating the main characteristics of the images to their corresponding manufacturing dates. Resolution methods followed by extracting features were able to estimate the tablet manufacture date with a prediction error of 120days. The model developed could be useful to evaluate whether a sample shows a degradation pattern consistent with the date of manufacturing or to detect abnormal behaviors in the natural degradation process of the sample. The information provided by the HIS-NIR is important for the development of the process (QbD), looking inside the formulation, revealing the behavior of the active pharmaceutical ingredient (API) during the product's shelf life. PMID:27163244

  12. Intraoperative brain hemodynamic response assessment with real-time hyperspectral optical imaging (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Laurence, Audrey; Pichette, Julien; Angulo-Rodríguez, Leticia M.; Saint Pierre, Catherine; Lesage, Frédéric; Bouthillier, Alain; Nguyen, Dang Khoa; Leblond, Frédéric

    2016-03-01

    Following normal neuronal activity, there is an increase in cerebral blood flow and cerebral blood volume to provide oxygenated hemoglobin to active neurons. For abnormal activity such as epileptiform discharges, this hemodynamic response may be inadequate to meet the high metabolic demands. To verify this hypothesis, we developed a novel hyperspectral imaging system able to monitor real-time cortical hemodynamic changes during brain surgery. The imaging system is directly integrated into a surgical microscope, using the white-light source for illumination. A snapshot hyperspectral camera is used for detection (4x4 mosaic filter array detecting 16 wavelengths simultaneously). We present calibration experiments where phantoms made of intralipid and food dyes were imaged. Relative concentrations of three dyes were recovered at a video rate of 30 frames per second. We also present hyperspectral recordings during brain surgery of epileptic patients with concurrent electrocorticography recordings. Relative concentration maps of oxygenated and deoxygenated hemoglobin were extracted from the data, allowing real-time studies of hemodynamic changes with a good spatial resolution. Finally, we present preliminary results on phantoms obtained with an integrated spatial frequency domain imaging system to recover tissue optical properties. This additional module, used together with the hyperspectral imaging system, will allow quantification of hemoglobin concentrations maps. Our hyperspectral imaging system offers a new tool to analyze hemodynamic changes, especially in the case of epileptiform discharges. It also offers an opportunity to study brain connectivity by analyzing correlations between hemodynamic responses of different tissue regions.

  13. NIR DLP hyperspectral imaging system for medical applications

    NASA Astrophysics Data System (ADS)

    Wehner, Eleanor; Thapa, Abhas; Livingston, Edward; Zuzak, Karel

    2011-03-01

    DLP® hyperspectral reflectance imaging in the visible range has been previously shown to quantify hemoglobin oxygenation in subsurface tissues, 1 mm to 2 mm deep. Extending the spectral range into the near infrared reflects biochemical information from deeper subsurface tissues. Unlike any other illumination method, the digital micro-mirror device, DMD, chip is programmable, allowing the user to actively illuminate with precisely predetermined spectra of illumination with a minimum bandpass of approximately 10 nm. It is possible to construct active spectral-based illumination that includes but is not limited to containing sharp cutoffs to act as filters or forming complex spectra, varying the intensity of light at discrete wavelengths. We have characterized and tested a pure NIR, 760 nm to 1600 nm, DLP hyperspectral reflectance imaging system. In its simplest application, the NIR system can be used to quantify the percentage of water in a subject, enabling edema visualization. It can also be used to map vein structure in a patient in real time. During gall bladder surgery, this system could be invaluable in imaging bile through fatty tissue, aiding surgeons in locating the common bile duct in real time without injecting any contrast agents.

  14. Development of practical thermal infrared hyperspectral imaging system

    NASA Astrophysics Data System (ADS)

    Wang, Jianyu; Li, Chunlai; Lv, Gang; Yuan, Liyin; Liu, Enguang; Jin, Jian; Ji, Hongzhen

    2014-11-01

    As an optical remote sensing equipment, the thermal infrared hyperspectral imager operates in the thermal infrared spectral band and acquires about 180 wavebands in range of 8.0~12.5μm. The field of view of this imager is 13° and the spatial resolution is better than 1mrad. Its noise equivalent temperature difference (NETD) is less than 0.2K@300K(average). 1 The influence of background radiation of the thermal infrared hyperspectral imager,and a simulation model of simplified background radiation is builded. 2 The design and implementationof the Cryogenic Optics. 3 Thermal infrared focal plane array (FPA) and special dewar component for the thermal infrared hyperspectral imager. 4 Parts of test results of the thermal infrared hyperspectral imager.The hyperspectral imaging system is China's first success in developing this type of instrument, whose flight validation experiments have already been embarked on. The thermal infrared hyperspectral data acquired will play an important role in fields such as geological exploration and air pollutant identification.

  15. Unmixing hyperspectral images using Markov random fields

    SciTech Connect

    Eches, Olivier; Dobigeon, Nicolas; Tourneret, Jean-Yves

    2011-03-14

    This paper proposes a new spectral unmixing strategy based on the normal compositional model that exploits the spatial correlations between the image pixels. The pure materials (referred to as endmembers) contained in the image are assumed to be available (they can be obtained by using an appropriate endmember extraction algorithm), while the corresponding fractions (referred to as abundances) are estimated by the proposed algorithm. Due to physical constraints, the abundances have to satisfy positivity and sum-to-one constraints. The image is divided into homogeneous distinct regions having the same statistical properties for the abundance coefficients. The spatial dependencies within each class are modeled thanks to Potts-Markov random fields. Within a Bayesian framework, prior distributions for the abundances and the associated hyperparameters are introduced. A reparametrization of the abundance coefficients is proposed to handle the physical constraints (positivity and sum-to-one) inherent to hyperspectral imagery. The parameters (abundances), hyperparameters (abundance mean and variance for each class) and the classification map indicating the classes of all pixels in the image are inferred from the resulting joint posterior distribution. To overcome the complexity of the joint posterior distribution, Markov chain Monte Carlo methods are used to generate samples asymptotically distributed according to the joint posterior of interest. Simulations conducted on synthetic and real data are presented to illustrate the performance of the proposed algorithm.

  16. Directly Estimating Endmembers for Compressive Hyperspectral Images

    PubMed Central

    Xu, Hongwei; Fu, Ning; Qiao, Liyan; Peng, Xiyuan

    2015-01-01

    The large volume of hyperspectral images (HSI) generated creates huge challenges for transmission and storage, making data compression more and more important. Compressive Sensing (CS) is an effective data compression technology that shows that when a signal is sparse in some basis, only a small number of measurements are needed for exact signal recovery. Distributed CS (DCS) takes advantage of both intra- and inter- signal correlations to reduce the number of measurements needed for multichannel-signal recovery. HSI can be observed by the DCS framework to reduce the volume of data significantly. The traditional method for estimating endmembers (spectral information) first recovers the images from the compressive HSI and then estimates endmembers via the recovered images. The recovery step takes considerable time and introduces errors into the estimation step. In this paper, we propose a novel method, by designing a type of coherent measurement matrix, to estimate endmembers directly from the compressively observed HSI data via convex geometry (CG) approaches without recovering the images. Numerical simulations show that the proposed method outperforms the traditional method with better estimation speed and better (or comparable) accuracy in both noisy and noiseless cases. PMID:25905699

  17. Autonomous, rapid classifiers for hyperspectral imagers

    NASA Astrophysics Data System (ADS)

    Gilmore, M. S.; Bornstein, B.; Castano, R.; Greenwood, J.

    2006-05-01

    Hyperspectral systems collect huge volumes of multidimensional data that require time consuming, expert analysis. The data analysis costs of global datasets restrict rapid classification to only a subset of an entire mission dataset, reducing mission science return. Data downlink restrictions from planetary missions also highlight the need for robust mineral detection algorithms. For example, both OMEGA and CRISM will map only approximately 5% of the Mars surface at full spatial and spectral resolution. While some targets are preselected for full resolution study, other high priority targets on Mars will be selected in response to observations made by the instruments in a multispectral survey mode. The challenge is to create mineral detection algorithms that can be utilized to analyze any and all image cubes (x, y, λ) for a selected system to help ensure that priority targets are not overlooked in these datasets. This goal is critical both for onboard, real time processing to direct target acquisition and for the mining of returned data. While an ultimate goal would be to accurately classify the composition of every pixel on a planet's surface, this is made difficult by the fact that most pixels are complex mixtures of n materials, which may or may not be represented in library (training) data. We instead focus on the identification of specific important mineral compositions within pixels in the data. For Mars, high priority targets include minerals associated with the presence of water. We have developed highly accurate artificial neural network (ANN) and Support Vector Machine (SVM) based detectors capable of identifying calcite (CaCO3) and jarosite (KFe3(SO4)2(OH)6) in the visible/NIR (350 to 2500 nm) spectra of both laboratory specimens and rocks in Mars analogue field environments. The detectors are trained using a generative model to create 1000s of linear mixtures of library end-member spectra in geologically realistic percentages. Here we will discuss

  18. Hyperspectral Image Classification using a Self-Organizing Map

    NASA Technical Reports Server (NTRS)

    Martinez, P.; Gualtieri, J. A.; Aguilar, P. L.; Perez, R. M.; Linaje, M.; Preciado, J. C.; Plaza, A.

    2001-01-01

    The use of hyperspectral data to determine the abundance of constituents in a certain portion of the Earth's surface relies on the capability of imaging spectrometers to provide a large amount of information at each pixel of a certain scene. Today, hyperspectral imaging sensors are capable of generating unprecedented volumes of radiometric data. The Airborne Visible/Infrared Imaging Spectrometer (AVIRIS), for example, routinely produces image cubes with 224 spectral bands. This undoubtedly opens a wide range of new possibilities, but the analysis of such a massive amount of information is not an easy task. In fact, most of the existing algorithms devoted to analyzing multispectral images are not applicable in the hyperspectral domain, because of the size and high dimensionality of the images. The application of neural networks to perform unsupervised classification of hyperspectral data has been tested by several authors and also by us in some previous work. We have also focused on analyzing the intrinsic capability of neural networks to parallelize the whole hyperspectral unmixing process. The results shown in this work indicate that neural network models are able to find clusters of closely related hyperspectral signatures, and thus can be used as a powerful tool to achieve the desired classification. The present work discusses the possibility of using a Self Organizing neural network to perform unsupervised classification of hyperspectral images. In sections 3 and 4, the topology of the proposed neural network and the training algorithm are respectively described. Section 5 provides the results we have obtained after applying the proposed methodology to real hyperspectral data, described in section 2. Different parameters in the learning stage have been modified in order to obtain a detailed description of their influence on the final results. Finally, in section 6 we provide the conclusions at which we have arrived.

  19. A hyperspectral imaging prototype for online quality evaluation of pickling cucumbers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral imaging prototype was developed for online evaluation of external and internal quality of pickling cucumbers. The prototype had several new, unique features including simultaneous reflectance and transmittance imaging and inline, real time calibration of hyperspectral images of each ...

  20. Improved Scanners for Microscopic Hyperspectral Imaging

    NASA Technical Reports Server (NTRS)

    Mao, Chengye

    2009-01-01

    Improved scanners to be incorporated into hyperspectral microscope-based imaging systems have been invented. Heretofore, in microscopic imaging, including spectral imaging, it has been customary to either move the specimen relative to the optical assembly that includes the microscope or else move the entire assembly relative to the specimen. It becomes extremely difficult to control such scanning when submicron translation increments are required, because the high magnification of the microscope enlarges all movements in the specimen image on the focal plane. To overcome this difficulty, in a system based on this invention, no attempt would be made to move either the specimen or the optical assembly. Instead, an objective lens would be moved within the assembly so as to cause translation of the image at the focal plane: the effect would be equivalent to scanning in the focal plane. The upper part of the figure depicts a generic proposed microscope-based hyperspectral imaging system incorporating the invention. The optical assembly of this system would include an objective lens (normally, a microscope objective lens) and a charge-coupled-device (CCD) camera. The objective lens would be mounted on a servomotor-driven translation stage, which would be capable of moving the lens in precisely controlled increments, relative to the camera, parallel to the focal-plane scan axis. The output of the CCD camera would be digitized and fed to a frame grabber in a computer. The computer would store the frame-grabber output for subsequent viewing and/or processing of images. The computer would contain a position-control interface board, through which it would control the servomotor. There are several versions of the invention. An essential feature common to all versions is that the stationary optical subassembly containing the camera would also contain a spatial window, at the focal plane of the objective lens, that would pass only a selected portion of the image. In one version

  1. Hyperspectral range imaging for transportation systems evaluation

    NASA Astrophysics Data System (ADS)

    Bridgelall, Raj; Rafert, J. B.; Atwood, Don; Tolliver, Denver D.

    2016-04-01

    Transportation agencies expend significant resources to inspect critical infrastructure such as roadways, railways, and pipelines. Regular inspections identify important defects and generate data to forecast maintenance needs. However, cost and practical limitations prevent the scaling of current inspection methods beyond relatively small portions of the network. Consequently, existing approaches fail to discover many high-risk defect formations. Remote sensing techniques offer the potential for more rapid and extensive non-destructive evaluations of the multimodal transportation infrastructure. However, optical occlusions and limitations in the spatial resolution of typical airborne and space-borne platforms limit their applicability. This research proposes hyperspectral image classification to isolate transportation infrastructure targets for high-resolution photogrammetric analysis. A plenoptic swarm of unmanned aircraft systems will capture images with centimeter-scale spatial resolution, large swaths, and polarization diversity. The light field solution will incorporate structure-from-motion techniques to reconstruct three-dimensional details of the isolated targets from sequences of two-dimensional images. A comparative analysis of existing low-power wireless communications standards suggests an application dependent tradeoff in selecting the best-suited link to coordinate swarming operations. This study further produced a taxonomy of specific roadway and railway defects, distress symptoms, and other anomalies that the proposed plenoptic swarm sensing system would identify and characterize to estimate risk levels.

  2. Camouflage target reconnaissance based on hyperspectral imaging technology

    NASA Astrophysics Data System (ADS)

    Hua, Wenshen; Guo, Tong; Liu, Xun

    2015-08-01

    Efficient camouflaged target reconnaissance technology makes great influence on modern warfare. Hyperspectral images can provide large spectral range and high spectral resolution, which are invaluable in discriminating between camouflaged targets and backgrounds. Hyperspectral target detection and classification technology are utilized to achieve single class and multi-class camouflaged targets reconnaissance respectively. Constrained energy minimization (CEM), a widely used algorithm in hyperspectral target detection, is employed to achieve one class camouflage target reconnaissance. Then, support vector machine (SVM), a classification method, is proposed to achieve multi-class camouflage target reconnaissance. Experiments have been conducted to demonstrate the efficiency of the proposed method.

  3. Meat quality evaluation by hyperspectral imaging technique: an overview.

    PubMed

    Elmasry, Gamal; Barbin, Douglas F; Sun, Da-Wen; Allen, Paul

    2012-01-01

    During the last two decades, a number of methods have been developed to objectively measure meat quality attributes. Hyperspectral imaging technique as one of these methods has been regarded as a smart and promising analytical tool for analyses conducted in research and industries. Recently there has been a renewed interest in using hyperspectral imaging in quality evaluation of different food products. The main inducement for developing the hyperspectral imaging system is to integrate both spectroscopy and imaging techniques in one system to make direct identification of different components and their spatial distribution in the tested product. By combining spatial and spectral details together, hyperspectral imaging has proved to be a promising technology for objective meat quality evaluation. The literature presented in this paper clearly reveals that hyperspectral imaging approaches have a huge potential for gaining rapid information about the chemical structure and related physical properties of all types of meat. In addition to its ability for effectively quantifying and characterizing quality attributes of some important visual features of meat such as color, quality grade, marbling, maturity, and texture, it is able to measure multiple chemical constituents simultaneously without monotonous sample preparation. Although this technology has not yet been sufficiently exploited in meat process and quality assessment, its potential is promising. Developing a quality evaluation system based on hyperspectral imaging technology to assess the meat quality parameters and to ensure its authentication would bring economical benefits to the meat industry by increasing consumer confidence in the quality of the meat products. This paper provides a detailed overview of the recently developed approaches and latest research efforts exerted in hyperspectral imaging technology developed for evaluating the quality of different meat products and the possibility of its widespread

  4. Hyperspectral image analysis using artificial color

    NASA Astrophysics Data System (ADS)

    Fu, Jian; Caulfield, H. John; Wu, Dongsheng; Tadesse, Wubishet

    2010-03-01

    By definition, HSC (HyperSpectral Camera) images are much richer in spectral data than, say, a COTS (Commercial-Off-The-Shelf) color camera. But data are not information. If we do the task right, useful information can be derived from the data in HSC images. Nature faced essentially the identical problem. The incident light is so complex spectrally that measuring it with high resolution would provide far more data than animals can handle in real time. Nature's solution was to do irreversible POCS (Projections Onto Convex Sets) to achieve huge reductions in data with minimal reduction in information. Thus we can arrange for our manmade systems to do what nature did - project the HSC image onto two or more broad, overlapping curves. The task we have undertaken in the last few years is to develop this idea that we call Artificial Color. What we report here is the use of the measured HSC image data projected onto two or three convex, overlapping, broad curves in analogy with the sensitivity curves of human cone cells. Testing two quite different HSC images in that manner produced the desired result: good discrimination or segmentation that can be done very simply and hence are likely to be doable in real time with specialized computers. Using POCS on the HSC data to reduce the processing complexity produced excellent discrimination in those two cases. For technical reasons discussed here, the figures of merit for the kind of pattern recognition we use is incommensurate with the figures of merit of conventional pattern recognition. We used some force fitting to make a comparison nevertheless, because it shows what is also obvious qualitatively. In our tasks our method works better.

  5. Hyperspectral image compression using bands combination wavelet transformation

    NASA Astrophysics Data System (ADS)

    Wang, Wenjie; Zhao, Zhongming; Zhu, Haiqing

    2009-10-01

    Hyperspectral imaging technology is the foreland of the remote sensing development in the 21st century and is one of the most important focuses of the remote sensing domain. Hyperspectral images can provide much more information than multispectral images do and can solve many problems which can't be solved by multispectral imaging technology. However this advantage is at the cost of massy quantity of data that brings difficulties of images' process, storage and transmission. Research on hyperspectral image compression method has important practical significance. This paper intends to do some improvement of the famous KLT-WT-2DSPECK (Karhunen-Loeve transform+ wavelet transformation+ two-dimensional set partitioning embedded block compression) algorithm and advances KLT + bands combination 2DWT + 2DSPECK algorithm. Experiment proves that this method is effective.

  6. Visible-Infrared Hyperspectral Image Projector

    NASA Technical Reports Server (NTRS)

    Bolcar, Matthew

    2013-01-01

    The VisIR HIP generates spatially-spectrally complex scenes. The generated scenes simulate real-world targets viewed by various remote sensing instruments. The VisIR HIP consists of two subsystems: a spectral engine and a spatial engine. The spectral engine generates spectrally complex uniform illumination that spans the wavelength range between 380 nm and 1,600 nm. The spatial engine generates two-dimensional gray-scale scenes. When combined, the two engines are capable of producing two-dimensional scenes with a unique spectrum at each pixel. The VisIR HIP can be used to calibrate any spectrally sensitive remote-sensing instrument. Tests were conducted on the Wide-field Imaging Interferometer Testbed at NASA s Goddard Space Flight Center. The device is a variation of the calibrated hyperspectral image projector developed by the National Institute of Standards and Technology in Gaithersburg, MD. It uses Gooch & Housego Visible and Infrared OL490 Agile Light Sources to generate arbitrary spectra. The two light sources are coupled to a digital light processing (DLP(TradeMark)) digital mirror device (DMD) that serves as the spatial engine. Scenes are displayed on the DMD synchronously with desired spectrum. Scene/spectrum combinations are displayed in rapid succession, over time intervals that are short compared to the integration time of the system under test.

  7. Raman Hyperspectral Imaging of Microfossils: Potential Pitfalls

    PubMed Central

    Olcott Marshall, Alison

    2013-01-01

    Abstract Initially, Raman spectroscopy was a specialized technique used by vibrational spectroscopists; however, due to rapid advancements in instrumentation and imaging techniques over the last few decades, Raman spectrometers are widely available at many institutions, allowing Raman spectroscopy to become a widespread analytical tool in mineralogy and other geological sciences. Hyperspectral imaging, in particular, has become popular due to the fact that Raman spectroscopy can quickly delineate crystallographic and compositional differences in 2-D and 3-D at the micron scale. Although this rapid growth of applications to the Earth sciences has provided great insight across the geological sciences, the ease of application as the instruments become increasingly automated combined with nonspecialists using this techique has resulted in the propagation of errors and misunderstandings throughout the field. For example, the literature now includes misassigned vibration modes, inappropriate spectral processing techniques, confocal depth of laser penetration incorrectly estimated into opaque crystalline solids, and a misconstrued understanding of the anisotropic nature of sp2 carbons. Key Words: Raman spectroscopy—Raman imaging—Confocal Raman spectroscopy—Disordered sp2 carbons—Hematite—Microfossils. Astrobiology 13, 920–931. PMID:24088070

  8. Hyperspectral fluorescence lifetime imaging for optical biopsy.

    PubMed

    Nie, Zhaojun; An, Ran; Hayward, Joseph E; Farrell, Thomas J; Fang, Qiyin

    2013-09-01

    A hyperspectral fluorescence lifetime imaging (FLIM) instrument is developed to study endogenous fluorophores in biological tissue as an optical biopsy tool. This instrument is able to spectrally, temporally, and spatially resolve fluorescence signal, thus providing multidimensional information to assist clinical tissue diagnosis. An acousto-optic tunable filter (AOTF) is used to realize rapid wavelength switch, and a photomultiplier tube and a high-speed digitizer are used to collect the time-resolved fluorescence decay at each wavelength in real time. The performance of this instrument has been characterized and validated on fluorescence tissue phantoms and fresh porcine skin specimens. This dual-arm AOTF design achieves high spectral throughput while allowing microsecond nonsequential, random wavelength switching, which is highly desirable for time-critical applications. In the results reported here, a motorized scanning stage is used to realize spatial scanning for two-dimensional images, while a rapid beam steering technique is feasible and being developed in an ongoing project. PMID:24002188

  9. Geographical classification of apple based on hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Guo, Zhiming; Huang, Wenqian; Chen, Liping; Zhao, Chunjiang; Peng, Yankun

    2013-05-01

    Attribute of apple according to geographical origin is often recognized and appreciated by the consumers. It is usually an important factor to determine the price of a commercial product. Hyperspectral imaging technology and supervised pattern recognition was attempted to discriminate apple according to geographical origins in this work. Hyperspectral images of 207 Fuji apple samples were collected by hyperspectral camera (400-1000nm). Principal component analysis (PCA) was performed on hyperspectral imaging data to determine main efficient wavelength images, and then characteristic variables were extracted by texture analysis based on gray level co-occurrence matrix (GLCM) from dominant waveband image. All characteristic variables were obtained by fusing the data of images in efficient spectra. Support vector machine (SVM) was used to construct the classification model, and showed excellent performance in classification results. The total classification rate had the high classify accuracy of 92.75% in the training set and 89.86% in the prediction sets, respectively. The overall results demonstrated that the hyperspectral imaging technique coupled with SVM classifier can be efficiently utilized to discriminate Fuji apple according to geographical origins.

  10. Hyperspectral imaging in the infrared using LIFTIRS. Revision 1

    SciTech Connect

    Bennett, C.L.; Carter, M.R.; Fields, D.J.

    1995-10-01

    In this article the ideal performance for various possible designs for imaging spectrometers is discussed. Recent characterization measurements made with LIFTIRS, the Livermore Imaging Fourier Transform InfraRed Spectrometer are also presented. Hyperspectral imagers, characterized by having a large number of spectral channels, enable definitive identification and quantitative measurement of the composition of objects in the field of view. Infrared hyperspectral imagers are particularly useful for remote chemical analysis, since almost all molecules have characteristic rotation-vibration spectra in the infrared, and a broad portion of the so-called fingerprint region of the infrared spectrum lies where the atmosphere is relatively transparent, between 8 and 13 {micro}m.

  11. Standoff midwave infrared hyperspectral imaging of ship plumes

    NASA Astrophysics Data System (ADS)

    Gagnon, Marc-André; Gagnon, Jean-Philippe; Tremblay, Pierre; Savary, Simon; Farley, Vincent; Guyot, Éric; Lagueux, Philippe; Chamberland, Martin; Marcotte, Frédérick

    2016-05-01

    Characterization of ship plumes is very challenging due to the great variety of ships, fuel, and fuel grades, as well as the extent of a gas plume. In this work, imaging of ship plumes from an operating ferry boat was carried out using standoff midwave (3-5 μm) infrared hyperspectral imaging. Quantitative chemical imaging of combustion gases was achieved by fitting a radiative transfer model. Combustion efficiency maps and mass flow rates are presented for carbon monoxide (CO) and carbon dioxide (CO2). The results illustrate how valuable information about the combustion process of a ship engine can be successfully obtained using passive hyperspectral remote sensing imaging.

  12. Classification of Korla fragrant pears using NIR hyperspectral imaging analysis

    NASA Astrophysics Data System (ADS)

    Rao, Xiuqin; Yang, Chun-Chieh; Ying, Yibin; Kim, Moon S.; Chao, Kuanglin

    2012-05-01

    Korla fragrant pears are small oval pears characterized by light green skin, crisp texture, and a pleasant perfume for which they are named. Anatomically, the calyx of a fragrant pear may be either persistent or deciduous; the deciduouscalyx fruits are considered more desirable due to taste and texture attributes. Chinese packaging standards require that packed cases of fragrant pears contain 5% or less of the persistent-calyx type. Near-infrared hyperspectral imaging was investigated as a potential means for automated sorting of pears according to calyx type. Hyperspectral images spanning the 992-1681 nm region were acquired using an EMCCD-based laboratory line-scan imaging system. Analysis of the hyperspectral images was performed to select wavebands useful for identifying persistent-calyx fruits and for identifying deciduous-calyx fruits. Based on the selected wavebands, an image-processing algorithm was developed that targets automated classification of Korla fragrant pears into the two categories for packaging purposes.

  13. Textural Analysis of Hyperspectral Images for Improving Contaminant Detection Accuracy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Previous studies demonstrated a hyperspectral imaging system has a potential for poultry fecal contaminant detection by measuring reflectance intensity. The simple image ratio at 565 and 517-nm images with optimal thresholding was able to detect fecal contaminants on broiler carcasses with high acc...

  14. Evaluating the Initialization Methods of Wavelet Networks for Hyperspectral Image Classification

    NASA Astrophysics Data System (ADS)

    Hsu, Pai-Hui

    2016-06-01

    The idea of using artificial neural network has been proven useful for hyperspectral image classification. However, the high dimensionality of hyperspectral images usually leads to the failure of constructing an effective neural network classifier. To improve the performance of neural network classifier, wavelet-based feature extraction algorithms can be applied to extract useful features for hyperspectral image classification. However, the extracted features with fixed position and dilation parameters of the wavelets provide insufficient characteristics of spectrum. In this study, wavelet networks which integrates the advantages of wavelet-based feature extraction and neural networks classification is proposed for hyperspectral image classification. Wavelet networks is a kind of feed-forward neural networks using wavelets as activation function. Both the position and the dilation parameters of the wavelets are optimized as well as the weights of the network during the training phase. The value of wavelet networks lies in their capabilities of optimizing network weights and extracting essential features simultaneously for hyperspectral images classification. In this study, the influence of the learning rate and momentum term during the network training phase is presented, and several initialization modes of wavelet networks were used to test the performance of wavelet networks.

  15. Land Cover Change Detection Based on Genetically Feature Aelection and Image Algebra Using Hyperion Hyperspectral Imagery

    NASA Astrophysics Data System (ADS)

    Seydi, S. T.; Hasanlou, M.

    2015-12-01

    The Earth has always been under the influence of population growth and human activities. This process causes the changes in land use. Thus, for optimal management of the use of resources, it is necessary to be aware of these changes. Satellite remote sensing has several advantages for monitoring land use/cover resources, especially for large geographic areas. Change detection and attribution of cultivation area over time present additional challenges for correctly analyzing remote sensing imagery. In this regards, for better identifying change in multi temporal images we use hyperspectral images. Hyperspectral images due to high spectral resolution created special placed in many of field. Nevertheless, selecting suitable and adequate features/bands from this data is crucial for any analysis and especially for the change detection algorithms. This research aims to automatically feature selection for detect land use changes are introduced. In this study, the optimal band images using hyperspectral sensor using Hyperion hyperspectral images by using genetic algorithms and Ratio bands, we select the optimal band. In addition, the results reveal the superiority of the implemented method to extract change map with overall accuracy by a margin of nearly 79% using multi temporal hyperspectral imagery.

  16. Hyperspectral forest monitoring and imaging implications

    NASA Astrophysics Data System (ADS)

    Goodenough, David G.; Bannon, David

    2014-05-01

    The forest biome is vital to the health of the earth. Canada and the United States have a combined forest area of 4.68 Mkm2. The monitoring of these forest resources has become increasingly complex. Hyperspectral remote sensing can provide a wealth of improved information products to land managers to make more informed decisions. Research in this area has demonstrated that hyperspectral remote sensing can be used to create more accurate products for forest inventory (major forest species), forest health, foliar biochemistry, biomass, and aboveground carbon. Operationally there is a requirement for a mix of airborne and satellite approaches. This paper surveys some methods and results in hyperspectral sensing of forests and discusses the implications for space initiatives with hyperspectral sensing

  17. Detecting Citrus Canker using Hyperspectral Reflectance Imaging and PCA-based Image Classification Method

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A portable hyperspectral imaging system was developed to measure the reflectance images from citrus samples with normal and various common diseased skin conditions in the wavelength range between 400 nm and 900 nm. PCA was used to reduce the spectral dimension of the 3-D hyperspectral image data and...

  18. Food inspection using hyperspectral imaging and SVDD

    NASA Astrophysics Data System (ADS)

    Uslu, Faruk Sukru; Binol, Hamidullah; Bal, Abdullah

    2016-05-01

    Nowadays food inspection and evaluation is becoming significant public issue, therefore robust, fast, and environmentally safe methods are studied instead of human visual assessment. Optical sensing is one of the potential methods with the properties of being non-destructive and accurate. As a remote sensing technology, hyperspectral imaging (HSI) is being successfully applied by researchers because of having both spatial and detailed spectral information about studied material. HSI can be used to inspect food quality and safety estimation such as meat quality assessment, quality evaluation of fish, detection of skin tumors on chicken carcasses, and classification of wheat kernels in the food industry. In this paper, we have implied an experiment to detect fat ratio in ground meat via Support Vector Data Description which is an efficient and robust one-class classifier for HSI. The experiments have been implemented on two different ground meat HSI data sets with different fat percentage. Addition to these implementations, we have also applied bagging technique which is mostly used as an ensemble method to improve the prediction ratio. The results show that the proposed methods produce high detection performance for fat ratio in ground meat.

  19. Resolving Mixed Algal Species in Hyperspectral Images

    PubMed Central

    Mehrubeoglu, Mehrube; Teng, Ming Y.; Zimba, Paul V.

    2014-01-01

    We investigated a lab-based hyperspectral imaging system's response from pure (single) and mixed (two) algal cultures containing known algae types and volumetric combinations to characterize the system's performance. The spectral response to volumetric changes in single and combinations of algal mixtures with known ratios were tested. Constrained linear spectral unmixing was applied to extract the algal content of the mixtures based on abundances that produced the lowest root mean square error. Percent prediction error was computed as the difference between actual percent volumetric content and abundances at minimum RMS error. Best prediction errors were computed as 0.4%, 0.4% and 6.3% for the mixed spectra from three independent experiments. The worst prediction errors were found as 5.6%, 5.4% and 13.4% for the same order of experiments. Additionally, Beer-Lambert's law was utilized to relate transmittance to different volumes of pure algal suspensions demonstrating linear logarithmic trends for optical property measurements. PMID:24451451

  20. Hyperspectral Imaging of fecal contamination on chickens

    NASA Technical Reports Server (NTRS)

    2003-01-01

    ProVision Technologies, a NASA research partnership center at Sternis Space Center in Mississippi, has developed a new hyperspectral imaging (HSI) system that is much smaller than the original large units used aboard remote sensing aircraft and satellites. The new apparatus is about the size of a breadbox. Health-related applications of HSI include scanning chickens during processing to help prevent contaminated food from getting to the table. ProVision is working with Sanderson Farms of Mississippi and the U.S. Department of Agriculture. ProVision has a record in its spectral library of the unique spectral signature of fecal contamination, so chickens can be scanned and those with a positive reading can be separated. HSI sensors can also determine the quantity of surface contamination. Research in this application is quite advanced, and ProVision is working on a licensing agreement for the technology. The potential for future use of this equipment in food processing and food safety is enormous.

  1. Random projection and SVD methods in hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Zhang, Jiani

    Hyperspectral imaging provides researchers with abundant information with which to study the characteristics of objects in a scene. Processing the massive hyperspectral imagery datasets in a way that efficiently provides useful information becomes an important issue. In this thesis, we consider methods which reduce the dimension of hyperspectral data while retaining as much useful information as possible. Traditional deterministic methods for low-rank approximation are not always adaptable to process huge datasets in an effective way, and therefore probabilistic methods are useful in dimension reduction of hyperspectral images. In this thesis, we begin by generally introducing the background and motivations of this work. Next, we summarize the preliminary knowledge and the applications of SVD and PCA. After these descriptions, we present a probabilistic method, randomized Singular Value Decomposition (rSVD), for the purposes of dimension reduction, compression, reconstruction, and classification of hyperspectral data. We discuss some variations of this method. These variations offer the opportunity to obtain a more accurate reconstruction of the matrix whose singular values decay gradually, to process matrices without target rank, and to obtain the rSVD with only one single pass over the original data. Moreover, we compare the method with Compressive-Projection Principle Component Analysis (CPPCA). From the numerical results, we can see that rSVD has better performance in compression and reconstruction than truncated SVD and CPPCA. We also apply rSVD to classification methods for the hyperspectral data provided by the National Geospatial-Intelligence Agency (NGA).

  2. a Diversified Deep Belief Network for Hyperspectral Image Classification

    NASA Astrophysics Data System (ADS)

    Zhong, P.; Gong, Z. Q.; Schönlieb, C.

    2016-06-01

    In recent years, researches in remote sensing demonstrated that deep architectures with multiple layers can potentially extract abstract and invariant features for better hyperspectral image classification. Since the usual real-world hyperspectral image classification task cannot provide enough training samples for a supervised deep model, such as convolutional neural networks (CNNs), this work turns to investigate the deep belief networks (DBNs), which allow unsupervised training. The DBN trained over limited training samples usually has many "dead" (never responding) or "potential over-tolerant" (always responding) latent factors (neurons), which decrease the DBN's description ability and thus finally decrease the hyperspectral image classification performance. This work proposes a new diversified DBN through introducing a diversity promoting prior over the latent factors during the DBN pre-training and fine-tuning procedures. The diversity promoting prior in the training procedures will encourage the latent factors to be uncorrelated, such that each latent factor focuses on modelling unique information, and all factors will be summed up to capture a large proportion of information and thus increase description ability and classification performance of the diversified DBNs. The proposed method was evaluated over the well-known real-world hyperspectral image dataset. The experiments demonstrate that the diversified DBNs can obtain much better results than original DBNs and comparable or even better performances compared with other recent hyperspectral image classification methods.

  3. In vivo and in vitro hyperspectral imaging of cervical neoplasia

    NASA Astrophysics Data System (ADS)

    Wang, Chaojian; Zheng, Wenli; Bu, Yanggao; Chang, Shufang; Tong, Qingping; Zhang, Shiwu; Xu, Ronald X.

    2014-02-01

    Cervical cancer is a prevalent disease in many developing countries. Colposcopy is the most common approach for screening cervical intraepithelial neoplasia (CIN). However, its clinical efficacy heavily relies on the examiner's experience. Spectroscopy is a potentially effective method for noninvasive diagnosis of cervical neoplasia. In this paper, we introduce a hyperspectral imaging technique for noninvasive detection and quantitative analysis of cervical neoplasia. A hyperspectral camera is used to collect the reflectance images of the entire cervix under xenon lamp illumination, followed by standard colposcopy examination and cervical tissue biopsy at both normal and abnormal sites in different quadrants. The collected reflectance data are calibrated and the hyperspectral signals are extracted. Further spectral analysis and image processing works are carried out to classify tissue into different types based on the spectral characteristics at different stages of cervical intraepithelial neoplasia. The hyperspectral camera is also coupled with a lab microscope to acquire the hyperspectral transmittance images of the pathological slides. The in vivo and the in vitro imaging results are compared with clinical findings to assess the accuracy and efficacy of the method.

  4. Hyperspectral retinal imaging with a spectrally tunable light source

    NASA Astrophysics Data System (ADS)

    Francis, Robert P.; Zuzak, Karel J.; Ufret-Vincenty, Rafael

    2011-03-01

    Hyperspectral retinal imaging can measure oxygenation and identify areas of ischemia in human patients, but the devices used by current researchers are inflexible in spatial and spectral resolution. We have developed a flexible research prototype consisting of a DLP®-based spectrally tunable light source coupled to a fundus camera to quickly explore the effects of spatial resolution, spectral resolution, and spectral range on hyperspectral imaging of the retina. The goal of this prototype is to (1) identify spectral and spatial regions of interest for early diagnosis of diseases such as glaucoma, age-related macular degeneration (AMD), and diabetic retinopathy (DR); and (2) define required specifications for commercial products. In this paper, we describe the challenges and advantages of using a spectrally tunable light source for hyperspectral retinal imaging, present clinical results of initial imaging sessions, and describe how this research can be leveraged into specifying a commercial product.

  5. Iterative compressive sampling for hyperspectral images via source separation

    NASA Astrophysics Data System (ADS)

    Kamdem Kuiteing, S.; Barni, Mauro

    2014-03-01

    Compressive Sensing (CS) is receiving increasing attention as a way to lower storage and compression requirements for on-board acquisition of remote-sensing images. In the case of multi- and hyperspectral images, however, exploiting the spectral correlation poses severe computational problems. Yet, exploiting such a correlation would provide significantly better performance in terms of reconstruction quality. In this paper, we build on a recently proposed 2D CS scheme based on blind source separation to develop a computationally simple, yet accurate, prediction-based scheme for acquisition and iterative reconstruction of hyperspectral images in a CS setting. Preliminary experiments carried out on different hyperspectral images show that our approach yields a dramatic reduction of computational time while ensuring reconstruction performance similar to those of much more complicated 3D reconstruction schemes.

  6. Hyperspectral imaging techniques for the characterization of Haematococcus pluvialis (Chlorophyceae).

    PubMed

    Nogami, Satoru; Ohnuki, Shinsuke; Ohya, Yoshikazu

    2014-10-01

    A hyperspectral imaging camera was combined with a bright-field microscope to investigate the intracellular distribution of pigments in cells of the green microalga Haematococcus pluvialis, a synonym for H. lacustris (Chlorophyceae). We applied multivariate curve resolution to the hyperspectral image data to estimate the pigment contents in culture and revealed that the predicted values were consistent with actual measurements obtained from extracted pigments. Because it was possible to estimate pigment contents in every pixel, the intracellular distribution of the pigments was investigated during various life-cycle stages. Astaxanthin was localized specifically at the eyespot of zoospores in early culture stages. Then, it became widely distributed in cells, but subsequently localized differently than the chl. Integrated with our recently developed image-processing program "HaematoCalMorph," the hyperspectral imaging system was useful for monitoring intracellular distributions of pigments during culture as well as for studying cellular responses under various conditions. PMID:26988647

  7. Diagnosis method of cucumber downy mildew with NIR hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Tian, Youwen; Li, Tianlai; Zhang, Lin; Zhang, Xiaodong

    2011-11-01

    This study was carried out to develop a hyperspectral imaging system in the near infrared (NIR) region (900-1700 nm) to diagnose cucumber downy mildew. Hyperspectral images were acquired from each diseased cucumber leaf samples with downy mildew and then their spectral data were extracted. Spectral data were analyzed using principal component analysis (PCA) to reduce the high dimensionality of the data and for selecting some important wavelengths. Out of 256 wavelengths, only two wavelengths (1426 and 1626nm) of first PC were selected as the optimum wavelengths for the diagnosis of cucumber downy mildew. The data analysis showed that it is possible to diagnose cucumber downy mildew with few numbers of wavelengths on the basis of their statistical image features and histogram features. The results revealed the potentiality of NIR hyperspectral imaging as an objective and non-destructive method for the authentication and diagnosis of cucumber downy mildew.

  8. Investigating coral hyperspectral properties across coral species and coral state using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Mehrubeoglu, Mehrube; Smith, Dustin K.; Smith, Shane W.; Strychar, Kevin B.; McLauchlan, Lifford

    2013-09-01

    Coral reefs are one of the most diverse and threatened ecosystems in the world. Corals worldwide are at risk, and in many instances, dying due to factors that affect their environment resulting in deteriorating environmental conditions. Because corals respond quickly to the quality of the environment that surrounds them, corals have been identified as bioindicators of water quality and marine environmental health. The hyperspectral imaging system is proposed as a noninvasive tool to monitor different species of corals as well as coral state over time. This in turn can be used as a quick and non-invasive method to monitor environmental health that can later be extended to climate conditions. In this project, a laboratory-based hyperspectral imaging system is used to collect spectral and spatial information of corals. In the work presented here, MATLAB and ENVI software tools are used to view and process spatial information and coral spectral signatures to identify differences among the coral data. The results support the hypothesis that hyperspectral properties of corals vary among different coral species, and coral state over time, and hyperspectral imaging can be a used as a tool to document changes in coral species and state.

  9. Atmospheric correction of hyperspectral images based on approximate solution of transmittance equation

    NASA Astrophysics Data System (ADS)

    Belov, A. M.; Myasnikov, V. V.

    2015-02-01

    The paper presents a method of atmospheric correction of remote sensing hyperspectral images. The method based on approximate solution of MODTRAN transmittance equation using simultaneous analysis of remote sensing hyperspectral image and "ideal" hyperspectral image which is free from atmospheric distortions. Experimental results show that proposed method is applicable to perform atmospheric correction.

  10. Hyperspectral Imaging Sensors and the Marine Coastal Zone

    NASA Technical Reports Server (NTRS)

    Richardson, Laurie L.

    2000-01-01

    Hyperspectral imaging sensors greatly expand the potential of remote sensing to assess, map, and monitor marine coastal zones. Each pixel in a hyperspectral image contains an entire spectrum of information. As a result, hyperspectral image data can be processed in two very different ways: by image classification techniques, to produce mapped outputs of features in the image on a regional scale; and by use of spectral analysis of the spectral data embedded within each pixel of the image. The latter is particularly useful in marine coastal zones because of the spectral complexity of suspended as well as benthic features found in these environments. Spectral-based analysis of hyperspectral (AVIRIS) imagery was carried out to investigate a marine coastal zone of South Florida, USA. Florida Bay is a phytoplankton-rich estuary characterized by taxonomically distinct phytoplankton assemblages and extensive seagrass beds. End-member spectra were extracted from AVIRIS image data corresponding to ground-truth sample stations and well-known field sites. Spectral libraries were constructed from the AVIRIS end-member spectra and used to classify images using the Spectral Angle Mapper (SAM) algorithm, a spectral-based approach that compares the spectrum, in each pixel of an image with each spectrum in a spectral library. Using this approach different phytoplankton assemblages containing diatoms, cyanobacteria, and green microalgae, as well as benthic community (seagrasses), were mapped.

  11. Overall design technology of hyperspectral imaging system based on AOTF

    NASA Astrophysics Data System (ADS)

    Wang, Jun; Ding, Na; Zheng, Yawei; Zhao, Yujie; Gao, Fei; Li, Junna; Wang, Jilong; Gao, Meng; Wu, Jun

    2014-11-01

    An acousto-optic tunable filter (AOTF) is an acousto-optic modulator. In this paper, the characteristics and overall design method of AOTF hyperspectral imaging system are proposed, which operates in visible or near infrared waveband (0.4-1.0um) and middle wave or long wave (3-5um and 8-12um). Compared with conventional dispersion element, the AOTF hyperspectral imaging system has a larger clear aperture because of the special characteristic of beam separation mode. In particularly, if the non-collinear design mode is used, the AOTF will have a larger diffraction aperture angle and is more suitable for the application in spectral imaging domain. The AOTF hyperspectral imaging spectrometer that operates in visible/near infrared waveband was developed by the non-collinear TeO2 crystal (8mm×8mm). All lights that are through TeO2 crystal in whole field of view (FOV angle is 5 degree) forms an imagines onto the staring focal plane array by Bragg diffraction. The diffraction wavelength of AOTF can be adjusted by the radio frequency signal. The three-dimensional data cube is composed of two-dimension of object space and wavelength in this way, and the graph and spectral are synthesized and implemented. The AOTF hyperspectral imaging spectrometer operating in visible/near infrared waveband is analyzed, and the detailed analysis data is also presented. The AOTF hyperspectral imaging test is studied and developed, and the analysis of data and the next developing advice is given. We also analyze the method about selection of material and technological design in middle wave/long wave infrared waveband of AOTF hyperspectral imaging system.

  12. Improved hyperspectral imaging system for fecal detection on poultry carcasses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Agricultural Research Service (ARS) has developed imaging technology to detect fecal contaminants on poultry carcasses. The hyperspectral imaging system operates from about 400 to 1000 nm, but only a few wavelengths are used in a real-time multispectral system. Recently, the upgraded system, inc...

  13. Phase correction algorithms for a snapshot hyperspectral imaging system

    NASA Astrophysics Data System (ADS)

    Chan, Victoria C.; Kudenov, Michael; Dereniak, Eustace

    2015-09-01

    We present image processing algorithms that improve spatial and spectral resolution on the Snapshot Hyperspectral Imaging Fourier Transform (SHIFT) spectrometer. Final measurements are stored in the form of threedimensional datacubes containing the scene's spatial and spectral information. We discuss calibration procedures, review post-processing methods, and present preliminary results from proof-of-concept experiments.

  14. LED lighting for use in multispectral and hyperspectral imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Lighting for machine vision and hyperspectral imaging is an important component for collecting high quality imagery. However, it is often given minimal consideration in the overall design of an imaging system. Tungsten-halogens lamps are the most common source of illumination for broad spectrum appl...

  15. Textural Analysis of Hyperspectral Images for Improving Detection Accuracy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Detection of fecal contamination is crucial for food safety to protect consumers from food pathogens. Previous studies demonstrated a hyperspectral imaging system has a potential for poultry fecal contaminant detection by measuring reflectance intensity. The simple image ratio with optimal thresho...

  16. Identification of seedling cabbages and weeds using hyperspectral imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Target detectionis one of research focues for precision chemical application. This study developed a method to identify seedling cabbages and weeds using hyperspectral spectral imaging. In processing the image data, with ENVI software, after dimension reduction, noise reduction, de-correlation for h...

  17. Spatial versus spectral compression ratio in compressive sensing of hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    August, Yitzhak; Vachman, Chaim; Stern, Adrian

    2013-05-01

    Compressive hyperspectral imaging is based on the fact that hyperspectral data is highly redundant. However, there is no symmetry between the compressibility of the spatial and spectral domains, and that should be taken into account for optimal compressive hyperspectral imaging system design. Here we present a study of the influence of the ratio between the compression in the spatial and spectral domains on the performance of a 3D separable compressive hyperspectral imaging method we recently developed.

  18. Content-based hyperspectral image retrieval using spectral unmixing

    NASA Astrophysics Data System (ADS)

    Plaza, Antonio J.

    2011-11-01

    The purpose of content-based image retrieval (CBIR) is to retrieve, from real data stored in a database, information that is relevant to a query. A major challenge for the development of efficient CBIR systems in the context of hyperspectral remote sensing applications is how to deal with the extremely large volumes of data produced by current Earth-observing (EO) imaging spectrometers. The data resulting from EO campaigns often comprises many Gigabytes per flight. When multiple instruments or timelines are combined, this leads to the collection of massive amounts of data coming from heterogeneous sources, and these data sets need to be effectively stored, managed, shared and retrieved. Furthermore, the growth in size and number of hyperspectral data archives demands more sophisticated search capabilities to allow users to locate and reuse data acquired in the past. In this paper we develop a new strategy to effectively retrieve hyperspectral image data sets using spectral unmixing concepts. Spectral unmixing is a very important task for hyperspectral data exploitation since the spectral signatures collected in natural environments are invariably a mixture of the pure signatures of the various materials found within the spatial extent of the ground instantaneous field view of the imaging instrument. In this work, we use the information provided by spectral unmixing (i.e. the spectral endmembers and their corresponding abundances in the scene) as effective meta-data to develop a new CBIR system that can assist users in the task of efficiently searching hyperspectral image instances in large data repositories. The proposed approach is validated using a collection of 154 hyperspectral data sets (comprising seven full flightlines) gathered by NASA using the Airborne Visible Infra-Red Imaging Spectrometer (AVIRIS) over the World Trade Center (WTC) area in New York City during the last two weeks of September, 2001, only a few days after the terrorist attacks that

  19. Hyperspectral imaging with a liquid crystal polarization interferometer.

    PubMed

    Hegyi, Alex; Martini, Joerg

    2015-11-01

    A novel hyperspectral imaging system has been developed that takes advantage of the tunable path delay between orthogonal polarization states of a liquid crystal variable retarder. The liquid crystal is placed in the optical path of an imaging system and the path delay between the polarization states is varied, causing an interferogram to be generated simultaneously at each pixel. A data set consisting of a series of images is recorded while varying the path delay; Fourier transforming the data set with respect to the path delay yields the hyperspectral data-cube. The concept is demonstrated with a prototype imager consisting of a liquid crystal variable retarder integrated into a commercial 640x480 pixel CMOS camera. The prototype can acquire a full hyperspectral data-cube in 0.4 s, and is sensitive to light over a 400 nm to 1100 nm range with a dispersion-dependent spectral resolution of 450 cm(-1) to 660 cm(-1). Similar to Fourier transform spectroscopy, the imager is spatially and spectrally multiplexed and therefore achieves high optical throughput. Additionally, the common-path nature of the polarization interferometer yields a vibration-insensitive device. Our concept allows for the spectral resolution, imaging speed, and spatial resolution to be traded off in software to optimally address a given application. The simplicity, compactness, potential low cost, and software adaptability of the device may enable a disruptive class of hyperspectral imaging systems with a broad range of applications. PMID:26561143

  20. Hyperspectral Image Super-Resolution via Non-Negative Structured Sparse Representation.

    PubMed

    Dong, Weisheng; Fu, Fazuo; Shi, Guangming; Cao, Xun; Wu, Jinjian; Li, Guangyu; Li, Guangyu

    2016-05-01

    Hyperspectral imaging has many applications from agriculture and astronomy to surveillance and mineralogy. However, it is often challenging to obtain high-resolution (HR) hyperspectral images using existing hyperspectral imaging techniques due to various hardware limitations. In this paper, we propose a new hyperspectral image super-resolution method from a low-resolution (LR) image and a HR reference image of the same scene. The estimation of the HR hyperspectral image is formulated as a joint estimation of the hyperspectral dictionary and the sparse codes based on the prior knowledge of the spatial-spectral sparsity of the hyperspectral image. The hyperspectral dictionary representing prototype reflectance spectra vectors of the scene is first learned from the input LR image. Specifically, an efficient non-negative dictionary learning algorithm using the block-coordinate descent optimization technique is proposed. Then, the sparse codes of the desired HR hyperspectral image with respect to learned hyperspectral basis are estimated from the pair of LR and HR reference images. To improve the accuracy of non-negative sparse coding, a clustering-based structured sparse coding method is proposed to exploit the spatial correlation among the learned sparse codes. The experimental results on both public datasets and real LR hypspectral images suggest that the proposed method substantially outperforms several existing HR hyperspectral image recovery techniques in the literature in terms of both objective quality metrics and computational efficiency. PMID:27019486

  1. Extended SWIR imaging sensors for hyperspectral imaging applications

    NASA Astrophysics Data System (ADS)

    Weber, A.; Benecke, M.; Wendler, J.; Sieck, A.; Hübner, D.; Figgemeier, H.; Breiter, R.

    2016-05-01

    AIM has developed SWIR modules including FPAs based on liquid phase epitaxy (LPE) grown MCT usable in a wide range of hyperspectral imaging applications. Silicon read-out integrated circuits (ROIC) provide various integration and readout modes including specific functions for spectral imaging applications. An important advantage of MCT based detectors is the tunable band gap. The spectral sensitivity of MCT detectors can be engineered to cover the extended SWIR spectral region up to 2.5μm without compromising in performance. AIM developed the technology to extend the spectral sensitivity of its SWIR modules also into the VIS. This has been successfully demonstrated for 384x288 and 1024x256 FPAs with 24μm pitch. Results are presented in this paper. The FPAs are integrated into compact dewar cooler configurations using different types of coolers, like rotary coolers, AIM's long life split linear cooler MCC030 or extreme long life SF100 Pulse Tube cooler. The SWIR modules include command and control electronics (CCE) which allow easy interfacing using a digital standard interface. The development status and performance results of AIM's latest MCT SWIR modules suitable for hyperspectral systems and applications will be presented.

  2. Spatial-scanning hyperspectral imaging probe for bio-imaging applications

    NASA Astrophysics Data System (ADS)

    Lim, Hoong-Ta; Murukeshan, Vadakke Matham

    2016-03-01

    The three common methods to perform hyperspectral imaging are the spatial-scanning, spectral-scanning, and snapshot methods. However, only the spectral-scanning and snapshot methods have been configured to a hyperspectral imaging probe as of today. This paper presents a spatial-scanning (pushbroom) hyperspectral imaging probe, which is realized by integrating a pushbroom hyperspectral imager with an imaging probe. The proposed hyperspectral imaging probe can also function as an endoscopic probe by integrating a custom fabricated image fiber bundle unit. The imaging probe is configured by incorporating a gradient-index lens at the end face of an image fiber bundle that consists of about 50 000 individual fiberlets. The necessary simulations, methodology, and detailed instrumentation aspects that are carried out are explained followed by assessing the developed probe's performance. Resolution test targets such as United States Air Force chart as well as bio-samples such as chicken breast tissue with blood clot are used as test samples for resolution analysis and for performance validation. This system is built on a pushbroom hyperspectral imaging system with a video camera and has the advantage of acquiring information from a large number of spectral bands with selectable region of interest. The advantages of this spatial-scanning hyperspectral imaging probe can be extended to test samples or tissues residing in regions that are difficult to access with potential diagnostic bio-imaging applications.

  3. Estimation of tissue optical parameters with hyperspectral imaging and spectral unmixing

    NASA Astrophysics Data System (ADS)

    Lu, Guolan; Qin, Xulei; Wang, Dongsheng; Chen, Zhuo G.; Fei, Baowei

    2015-03-01

    Early detection of oral cancer and its curable precursors can improve patient survival and quality of life. Hyperspectral imaging (HSI) holds the potential for noninvasive early detection of oral cancer. The quantification of tissue chromophores by spectral unmixing of hyperspectral images could provide insights for evaluating cancer progression. In this study, non-negative matrix factorization has been applied for decomposing hyperspectral images into physiologically meaningful chromophore concentration maps. The approach has been validated by computer-simulated hyperspectral images and in vivo tumor hyperspectral images from a head and neck cancer animal model.

  4. Estimation of Tissue Optical Parameters with Hyperspectral Imaging and Spectral Unmixing

    PubMed Central

    Lu, Guolan; Qin, Xulei; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

    2015-01-01

    Early detection of oral cancer and its curable precursors can improve patient survival and quality of life. Hyperspectral imaging (HSI) holds the potential for noninvasive early detection of oral cancer. The quantification of tissue chromophores by spectral unmixing of hyperspectral images could provide insights for evaluating cancer progression. In this study, non-negative matrix factorization has been applied for decomposing hyperspectral images into physiologically meaningful chromophore concentration maps. The approach has been validated by computer-simulated hyperspectral images and in vivo tumor hyperspectral images from a head and neck cancer animal model. PMID:26855467

  5. Fault tolerance of SVM algorithm for hyperspectral image

    NASA Astrophysics Data System (ADS)

    Cui, Yabo; Yuan, Zhengwu; Wu, Yuanfeng; Gao, Lianru; Zhang, Hao

    2015-10-01

    One of the most important tasks in analyzing hyperspectral image data is the classification process[1]. In general, in order to enhance the classification accuracy, a data preprocessing step is usually adopted to remove the noise in the data before classification. But for the time-sensitive applications, we hope that even the data contains noise the classifier can still appear to execute correctly from the user's perspective, such as risk prevention and response. As the most popular classifier, Support Vector Machine (SVM) has been widely used for hyperspectral image classification and proved to be a very promising technique in supervised classification[2]. In this paper, two experiments are performed to demonstrate that for the hyperspectral data with noise, if the noise of the data is within a certain range, SVM algorithm is still able to execute correctly from the user's perspective.

  6. Spectral-Spatial Classification of Hyperspectral Images Using Hierarchical Optimization

    NASA Technical Reports Server (NTRS)

    Tarabalka, Yuliya; Tilton, James C.

    2011-01-01

    A new spectral-spatial method for hyperspectral data classification is proposed. For a given hyperspectral image, probabilistic pixelwise classification is first applied. Then, hierarchical step-wise optimization algorithm is performed, by iteratively merging neighboring regions with the smallest Dissimilarity Criterion (DC) and recomputing class labels for new regions. The DC is computed by comparing region mean vectors, class labels and a number of pixels in the two regions under consideration. The algorithm is converged when all the pixels get involved in the region merging procedure. Experimental results are presented on two remote sensing hyperspectral images acquired by the AVIRIS and ROSIS sensors. The proposed approach improves classification accuracies and provides maps with more homogeneous regions, when compared to previously proposed classification techniques.

  7. Hyperspectral Imaging and Related Field Methods: Building the Science

    NASA Technical Reports Server (NTRS)

    Goetz, Alexander F. H.; Steffen, Konrad; Wessman, Carol

    1999-01-01

    The proposal requested funds for the computing power to bring hyperspectral image processing into undergraduate and graduate remote sensing courses. This upgrade made it possible to handle more students in these oversubscribed courses and to enhance CSES' summer short course entitled "Hyperspectral Imaging and Data Analysis" provided for government, industry, university and military. Funds were also requested to build field measurement capabilities through the purchase of spectroradiometers, canopy radiation sensors and a differential GPS system. These instruments provided systematic and complete sets of field data for the analysis of hyperspectral data with the appropriate radiometric and wavelength calibration as well as atmospheric data needed for application of radiative transfer models. The proposed field equipment made it possible to team-teach a new field methods course, unique in the country, that took advantage of the expertise of the investigators rostered in three different departments, Geology, Geography and Biology.

  8. Combining hyperspectral imaging and Raman spectroscopy for remote chemical sensing

    NASA Astrophysics Data System (ADS)

    Ingram, John M.; Lo, Edsanter

    2008-04-01

    The Photonics Research Center at the United States Military Academy is conducting research to demonstrate the feasibility of combining hyperspectral imaging and Raman spectroscopy for remote chemical detection over a broad area of interest. One limitation of future trace detection systems is their ability to analyze large areas of view. Hyperspectral imaging provides a balance between fast spectral analysis and scanning area. Integration of a hyperspectral system capable of remote chemical detection will greatly enhance our soldiers' ability to see the battlefield to make threat related decisions. It can also queue the trace detection systems onto the correct interrogation area saving time and reconnaissance/surveillance resources. This research develops both the sensor design and the detection/discrimination algorithms. The one meter remote detection without background radiation is a simple proof of concept.

  9. Advances in Spectral-Spatial Classification of Hyperspectral Images

    NASA Technical Reports Server (NTRS)

    Fauvel, Mathieu; Tarabalka, Yuliya; Benediktsson, Jon Atli; Chanussot, Jocelyn; Tilton, James C.

    2012-01-01

    Recent advances in spectral-spatial classification of hyperspectral images are presented in this paper. Several techniques are investigated for combining both spatial and spectral information. Spatial information is extracted at the object (set of pixels) level rather than at the conventional pixel level. Mathematical morphology is first used to derive the morphological profile of the image, which includes characteristics about the size, orientation and contrast of the spatial structures present in the image. Then the morphological neighborhood is defined and used to derive additional features for classification. Classification is performed with support vector machines using the available spectral information and the extracted spatial information. Spatial post-processing is next investigated to build more homogeneous and spatially consistent thematic maps. To that end, three presegmentation techniques are applied to define regions that are used to regularize the preliminary pixel-wise thematic map. Finally, a multiple classifier system is defined to produce relevant markers that are exploited to segment the hyperspectral image with the minimum spanning forest algorithm. Experimental results conducted on three real hyperspectral images with different spatial and spectral resolutions and corresponding to various contexts are presented. They highlight the importance of spectral-spatial strategies for the accurate classification of hyperspectral images and validate the proposed methods.

  10. Hyperspectral imaging and quantitative analysis for prostate cancer detection

    PubMed Central

    Akbari, Hamed; Halig, Luma V.; Schuster, David M.; Osunkoya, Adeboye; Master, Viraj; Nieh, Peter T.; Chen, Georgia Z.

    2012-01-01

    Abstract. Hyperspectral imaging (HSI) is an emerging modality for various medical applications. Its spectroscopic data might be able to be used to noninvasively detect cancer. Quantitative analysis is often necessary in order to differentiate healthy from diseased tissue. We propose the use of an advanced image processing and classification method in order to analyze hyperspectral image data for prostate cancer detection. The spectral signatures were extracted and evaluated in both cancerous and normal tissue. Least squares support vector machines were developed and evaluated for classifying hyperspectral data in order to enhance the detection of cancer tissue. This method was used to detect prostate cancer in tumor-bearing mice and on pathology slides. Spatially resolved images were created to highlight the differences of the reflectance properties of cancer versus those of normal tissue. Preliminary results with 11 mice showed that the sensitivity and specificity of the hyperspectral image classification method are 92.8% to 2.0% and 96.9% to 1.3%, respectively. Therefore, this imaging method may be able to help physicians to dissect malignant regions with a safe margin and to evaluate the tumor bed after resection. This pilot study may lead to advances in the optical diagnosis of prostate cancer using HSI technology. PMID:22894488

  11. Hyperspectral imaging and quantitative analysis for prostate cancer detection

    NASA Astrophysics Data System (ADS)

    Akbari, Hamed; Halig, Luma V.; Schuster, David M.; Osunkoya, Adeboye; Master, Viraj; Nieh, Peter T.; Chen, Georgia Z.; Fei, Baowei

    2012-07-01

    Hyperspectral imaging (HSI) is an emerging modality for various medical applications. Its spectroscopic data might be able to be used to noninvasively detect cancer. Quantitative analysis is often necessary in order to differentiate healthy from diseased tissue. We propose the use of an advanced image processing and classification method in order to analyze hyperspectral image data for prostate cancer detection. The spectral signatures were extracted and evaluated in both cancerous and normal tissue. Least squares support vector machines were developed and evaluated for classifying hyperspectral data in order to enhance the detection of cancer tissue. This method was used to detect prostate cancer in tumor-bearing mice and on pathology slides. Spatially resolved images were created to highlight the differences of the reflectance properties of cancer versus those of normal tissue. Preliminary results with 11 mice showed that the sensitivity and specificity of the hyperspectral image classification method are 92.8% to 2.0% and 96.9% to 1.3%, respectively. Therefore, this imaging method may be able to help physicians to dissect malignant regions with a safe margin and to evaluate the tumor bed after resection. This pilot study may lead to advances in the optical diagnosis of prostate cancer using HSI technology.

  12. Advances in Hyperspectral and Multispectral Image Fusion and Spectral Unmixing

    NASA Astrophysics Data System (ADS)

    Lanaras, C.; Baltsavias, E.; Schindler, K.

    2015-08-01

    In this work, we jointly process high spectral and high geometric resolution images and exploit their synergies to (a) generate a fused image of high spectral and geometric resolution; and (b) improve (linear) spectral unmixing of hyperspectral endmembers at subpixel level w.r.t. the pixel size of the hyperspectral image. We assume that the two images are radiometrically corrected and geometrically co-registered. The scientific contributions of this work are (a) a simultaneous approach to image fusion and hyperspectral unmixing, (b) enforcing several physically plausible constraints during unmixing that are all well-known, but typically not used in combination, and (c) the use of efficient, state-of-the-art mathematical optimization tools to implement the processing. The results of our joint fusion and unmixing has the potential to enable more accurate and detailed semantic interpretation of objects and their properties in hyperspectral and multispectral images, with applications in environmental mapping, monitoring and change detection. In our experiments, the proposed method always improves the fusion compared to competing methods, reducing RMSE between 4% and 53%.

  13. Hyperspectral imaging and quantitative analysis for prostate cancer detection.

    PubMed

    Akbari, Hamed; Halig, Luma V; Schuster, David M; Osunkoya, Adeboye; Master, Viraj; Nieh, Peter T; Chen, Georgia Z; Fei, Baowei

    2012-07-01

    Hyperspectral imaging (HSI) is an emerging modality for various medical applications. Its spectroscopic data might be able to be used to noninvasively detect cancer. Quantitative analysis is often necessary in order to differentiate healthy from diseased tissue. We propose the use of an advanced image processing and classification method in order to analyze hyperspectral image data for prostate cancer detection. The spectral signatures were extracted and evaluated in both cancerous and normal tissue. Least squares support vector machines were developed and evaluated for classifying hyperspectral data in order to enhance the detection of cancer tissue. This method was used to detect prostate cancer in tumor-bearing mice and on pathology slides. Spatially resolved images were created to highlight the differences of the reflectance properties of cancer versus those of normal tissue. Preliminary results with 11 mice showed that the sensitivity and specificity of the hyperspectral image classification method are 92.8% to 2.0% and 96.9% to 1.3%, respectively. Therefore, this imaging method may be able to help physicians to dissect malignant regions with a safe margin and to evaluate the tumor bed after resection. This pilot study may lead to advances in the optical diagnosis of prostate cancer using HSI technology. PMID:22894488

  14. a Review of Hyperspectral Imaging in Close Range Applications

    NASA Astrophysics Data System (ADS)

    Kurz, T. H.; Buckley, S. J.

    2016-06-01

    Hyperspectral imaging is an established method for material mapping, which has been conventionally applied from airborne and spaceborne platforms for a range of applications, including mineral and vegetation mapping, change detection and environmental studies. The main advantage of lightweight hyperspectral imagers lies in the flexibility to deploy them from various platforms (terrestrial imaging and from unmanned aerial vehicles; UAVs), as well as the high spectral resolution to cover an expanding wavelength range. In addition, spatial resolution allows object sampling distances from micrometres to tens of centimetres - complementary to conventional nadir-looking systems. When this new type of imaging device was initially released, few instruments were available and the applicability and potential of the method was restricted. Today, a wider range of instruments, with a range of specifications, is available, with significant improvements over the first generation of technology. In this contribution, the state-of-the-art of hyperspectral imaging will be reviewed from a close range measurement perspective, highlighting how the method supplements geometric modelling techniques. An overview of the processing workflow, adjusted to the more complex close range imaging scenario will be given. This includes the integration with 3D laser scanning and photogrammetric models to provide a geometric framework and real world coordinate system for the hyperspectral imagery.

  15. Hyperspectral and Multispectral Imaging Technique for Food Quality and Safety Evaluation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this chapter, recently developed ARS line-scan hyperspectral-based sensing technologies to address agro-food safety concerns are presented including a case study using the laboratory-based hyperspectral imaging platforms. An online line-scan imaging system capable of both hyperspectral and multi...

  16. Detection of cracks on tomatoes using hyperspectral near-infrared reflectance imaging system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of this study was to evaluate the use of hyperspectral near-infrared (NIR) reflectance imaging techniques for detection of cuticle cracks on tomatoes. A hyperspectral near-infrared reflectance imaging system in the region of 1000-1700 nm was used to obtain hyperspectral reflectance ima...

  17. Hyperspectral imaging of an inter-coastal waterway

    NASA Astrophysics Data System (ADS)

    Bowles, Jeffrey H.; Maness, Shelia J.; Chen, Wei; Davis, Curtiss O.; Donato, Tim F.; Gillis, David B.; Korwan, Daniel; Lamela, Gia; Montes, Marcos J.; Rhea, W. Joseph; Snyder, William A.

    2005-10-01

    This paper demonstrates the characterization of the water properties, bathymetry, and bottom type of the Indian River Lagoon (IRL) on the eastern coast of Florida using hyperspectral imagery. Images of this region were collected from an aircraft in July 2004 using the Portable Hyperspectral Imager for Low Light Spectroscopy (PHILLS). PHILLS is a Visible Near InfraRed (VNIR) spectrometer that was operated at an altitude of 3000 m providing 4 m resolution with 128 bands from 400 to 1000 nm. The IRL is a well studied water body that receives fresh water drainage from the Florida Everglades and also tidal driven flushing of ocean water through several outlets in the barrier islands. Ground truth measurements of the bathymetry of IRL were acquired from recent sonar and LIDAR bathymetry maps as well as water quality studies concurrent to the hyperspectral data collections. From these measurements, bottom types are known to include sea grass, various algae, and a gray mud with water depths less than 6 m over most of the lagoon. Suspended sediments are significant (~35 mg/m3) with chlorophyll levels less than 10 mg/m3 while the absorption due to Colored Dissolved Organic Matter (CDOM) is less than 1 m-1 at 440 nm. Hyperspectral data were atmospherically corrected using an NRL software package called Tafkaa and then subjected to a Look-Up Table (LUT) approach which matches hyperspectral data to calculated spectra with known values for bathymetry, suspended sediments, chlorophyll, CDOM, and bottom type.

  18. Hyperspectral image analysis for plant stress detection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Abiotic and disease-induced stress significantly reduces plant productivity. Automated on-the-go mapping of plant stress allows timely intervention and mitigating of the problem before critical thresholds are exceeded, thereby, maximizing productivity. A hyperspectral camera analyzed the spectral ...

  19. Study on classification of pork quality using hyperspectral imaging technique

    NASA Astrophysics Data System (ADS)

    Zeng, Shan; Bai, Jun; Wang, Haibin

    2015-12-01

    The relative problems' research of chilled meat, thawed meat and spoiled meat discrimination by hyperspectral image technique were proposed, such the section of feature wavelengths, et al. First, based on 400 ~ 1000nm range hyperspectral image data of testing pork samples, by K-medoids clustering algorithm based on manifold distance, we select 30 important wavelengths from 753 wavelengths, and thus select 8 feature wavelengths (454.4, 477.5, 529.3, 546.8, 568.4, 580.3, 589.9 and 781.2nm) based on the discrimination value. Then 8 texture features of each image under 8 feature wavelengths were respectively extracted by two-dimensional Gabor wavelets transform as pork quality feature. Finally, we build a pork quality classification model using the fuzzy C-mean clustering algorithm. Through the experiment of extracting feature wavelengths, we found that although the hyperspectral images between adjacent bands have a strong linear correlation, they show a significant non-linear manifold relationship from the entire band. K-medoids clustering algorithm based on manifold distance used in this paper for selecting the characteristic wavelengths, which is more reasonable than traditional principal component analysis (PCA). Through the classification result, we conclude that hyperspectral imaging technology can distinguish among chilled meat, thawed meat and spoiled meat accurately.

  20. Algorithm for mapping cutaneous tissue oxygen concentration using hyperspectral imaging

    PubMed Central

    Miclos, Sorin; Parasca, Sorin Viorel; Calin, Mihaela Antonina; Savastru, Dan; Manea, Dragos

    2015-01-01

    The measurement of tissue oxygenation plays an important role in the diagnosis and therapeutic assessment of a large variety of diseases. Many different methods have been developed and are currently applied in clinical practice for the measurement of tissue oxygenation. Unfortunately, each of these methods has its own limitations. In this paper we proposed the use of hyperspectral imaging as new method for the assessment of the tissue oxygenation level. To extract this information from hyperspectral images a new algorithm for mapping cutaneous tissue oxygen concentration was developed. This algorithm takes into account and solves some problems related to setting and calculation of some parameters derived from hyperspectral images. The algorithm was tested with good results on synthetic images and then validated on the fingers of a hand with different blood irrigation states. The results obtained have proved the ability of hyperspectral imaging together with the developed algorithm to map the oxy- and deoxyhemoglobin distribution on the analyzed fingers. These are only preliminary results and other studies should be done before this approach to be used in the clinical setting for the diagnosis and monitoring of various diseases. PMID:26417511

  1. Developing digital tissue phantoms for hyperspectral imaging of ischemic wounds.

    PubMed

    Xu, Ronald X; Allen, David W; Huang, Jiwei; Gnyawali, Surya; Melvin, James; Elgharably, Haytham; Gordillo, Gayle; Huang, Kun; Bergdall, Valerie; Litorja, Maritoni; Rice, Joseph P; Hwang, Jeeseong; Sen, Chandan K

    2012-06-01

    Hyperspectral imaging has the potential to achieve high spatial resolution and high functional sensitivity for non-invasive assessment of tissue oxygenation. However, clinical acceptance of hyperspectral imaging in ischemic wound assessment is hampered by its poor reproducibility, low accuracy, and misinterpreted biology. These limitations are partially caused by the lack of a traceable calibration standard. We proposed a digital tissue phantom (DTP) platform for quantitative calibration and performance evaluation of spectral wound imaging devices. The technical feasibility of such a DTP platform was demonstrated by both in vitro and in vivo experiments. The in vitro DTPs were developed based on a liquid blood phantom model. The in vivo DTPs were developed based on a porcine ischemic skin flap model. The DTPs were projected by a Hyperspectral Image Projector (HIP) with high fidelity. A wide-gap 2nd derivative oxygenation algorithm was developed to reconstruct tissue functional parameters from hyperspectral measurements. In this study, we have demonstrated not only the technical feasibility of using DTPs for quantitative calibration, evaluation, and optimization of spectral imaging devices but also its potential for ischemic wound assessment in clinical practice. PMID:22741088

  2. Hyperspectral imaging system for whole corn ear surface inspection

    NASA Astrophysics Data System (ADS)

    Yao, Haibo; Kincaid, Russell; Hruska, Zuzana; Brown, Robert L.; Bhatnagar, Deepak; Cleveland, Thomas E.

    2013-05-01

    Aflatoxin is a mycotoxin produced mainly by Aspergillus flavus (A.flavus) and Aspergillus parasitiucus fungi that grow naturally in corn. Very serious health problems such as liver damage and lung cancer can result from exposure to high toxin levels in grain. Consequently, many countries have established strict guidelines for permissible levels in consumables. Conventional chemical-based analytical methods used to screen for aflatoxin such as thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) are time consuming, expensive, and require the destruction of samples as well as proper training for data interpretation. Thus, it has been a continuing effort within the research community to find a way to rapidly and non-destructively detect and possibly quantify aflatoxin contamination in corn. One of the more recent developments in this area is the use of spectral technology. Specifically, fluorescence hyperspectral imaging offers a potential rapid, and non-invasive method for contamination detection in corn infected with toxigenic A.flavus spores. The current hyperspectral image system is designed for scanning flat surfaces, which is suitable for imaging single or a group of corn kernels. In the case of a whole corn cob, it is preferred to be able to scan the circumference of the corn ear, appropriate for whole ear inspection. This paper discusses the development of a hyperspectral imaging system for whole corn ear imaging. The new instrument is based on a hyperspectral line scanner using a rotational stage to turn the corn ear.

  3. Fast Hyperspectral Imaging Using a Mid-Infrared Tunable External Cavity Quantum Cascade Laser

    SciTech Connect

    Phillips, Mark C.; Ho, Nicolas

    2008-04-23

    An active hyperspectral imaging system using an external cavity quantum cascade laser and a focal plane array acquiring images at 25 Hz from 985 cm-1 to 1075 cm-1 with a resolution of 0.3 cm 1 is demonstrated. The chemical imaging of gases is demonstrated in both static and dynamic cases. The system was also used to analyze liquid and solid samples.

  4. Context Modeler for Wavelet Compression of Spectral Hyperspectral Images

    NASA Technical Reports Server (NTRS)

    Kiely, Aaron; Xie, Hua; Klimesh, matthew; Aranki, Nazeeh

    2010-01-01

    A context-modeling sub-algorithm has been developed as part of an algorithm that effects three-dimensional (3D) wavelet-based compression of hyperspectral image data. The context-modeling subalgorithm, hereafter denoted the context modeler, provides estimates of probability distributions of wavelet-transformed data being encoded. These estimates are utilized by an entropy coding subalgorithm that is another major component of the compression algorithm. The estimates make it possible to compress the image data more effectively than would otherwise be possible. The following background discussion is prerequisite to a meaningful summary of the context modeler. This discussion is presented relative to ICER-3D, which is the name attached to a particular compression algorithm and the software that implements it. The ICER-3D software is summarized briefly in the preceding article, ICER-3D Hyperspectral Image Compression Software (NPO-43238). Some aspects of this algorithm were previously described, in a slightly more general context than the ICER-3D software, in "Improving 3D Wavelet-Based Compression of Hyperspectral Images" (NPO-41381), NASA Tech Briefs, Vol. 33, No. 3 (March 2009), page 7a. In turn, ICER-3D is a product of generalization of ICER, another previously reported algorithm and computer program that can perform both lossless and lossy wavelet-based compression and decompression of gray-scale-image data. In ICER-3D, hyperspectral image data are decomposed using a 3D discrete wavelet transform (DWT). Following wavelet decomposition, mean values are subtracted from spatial planes of spatially low-pass subbands prior to encoding. The resulting data are converted to sign-magnitude form and compressed. In ICER-3D, compression is progressive, in that compressed information is ordered so that as more of the compressed data stream is received, successive reconstructions of the hyperspectral image data are of successively higher overall fidelity.

  5. Snapshot hyperspectral retinal camera with the Image Mapping Spectrometer (IMS)

    PubMed Central

    Gao, Liang; Smith, R. Theodore; Tkaczyk, Tomasz S.

    2011-01-01

    We present a snapshot hyperspectral retinal camera with the Image Mapping Spectrometer (IMS) for eye imaging applications. The resulting system is capable of simultaneously acquiring 48 spectral channel images in the range 470 nm–650 nm with frame rate at 5.2 fps. The spatial sampling of each measured spectral scene is 350 × 350 pixels. The advantages of this snapshot device are elimination of the eye motion artifacts and pixel misregistration problems in traditional scanning-based hyperspectral retinal cameras, and real-time imaging of oxygen saturation dynamics with sub-second temporal resolution. The spectral imaging performance is demonstrated in a human retinal imaging experiment in vivo. The absorption spectral signatures of oxy-hemoglobin and macular pigments were successfully acquired by using this device. PMID:22254167

  6. Methodology for hyperspectral image classification using novel neural network

    SciTech Connect

    Subramanian, S., Gat, N., Sheffield, M.,; Barhen, J.; Toomarian, N.

    1997-04-01

    A novel feed forward neural network is used to classify hyperspectral data from the AVIRIS sector. The network applies an alternating direction singular value decomposition technique to achieve rapid training times (few seconds per class). Very few samples (10-12) are required for training. 100% accurate classification is obtained using test data sets. The methodology combines this rapid training neural network together with data reduction and maximal feature separation techniques such as principal component analysis and simultaneous diagonalization of covariance matrices, for rapid and accurate classification of large hyperspectral images. The results are compared to those of standard statistical classifiers. 21 refs., 3 figs., 5 tabs.

  7. AOTF hyperspectral microscope imaging for foodborne pathogenic bacteria detection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral microscope imaging (HMI) method, which provides both spatial and spectral information, can be effective for foodborne pathogen detection. The acousto-optic tunable filter (AOTF)-based HMI method can be used to characterize spectral properties of biofilms formed by Salmonella enteritidi...

  8. Detection of lettuce discoloration using hyperspectral reflectance imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rapid visible/near-infrared (VNIR) hyperspectral imaging methods, employing both a single waveband algorithm and multi-spectral algorithms, were developed in order to classify the discoloration of lettuce. Reflectance spectra for sound and discolored lettuce surfaces were extracted from hyperspectra...

  9. Portable hyperspectral imager for assessment of skin disorders: preliminary measurements

    NASA Astrophysics Data System (ADS)

    Beach, James M.; Lanoue, Mark A.; Brabham, Kori; Khoobehi, Bahram

    2005-04-01

    Oxygenation of the facial skin was evaluated in rosacea using a hyperspectral camera. A portable imaging system utilizing crossed-polarization optics for illumination and recording is described. Relative oxygen saturation was determined from rosacea features and compared with normal skin. Saturation maps and light absorption spectra showed a significant increase in the oxygen saturation of the blood in rosacea-affected skin.

  10. Hyperspectral Reflectance Imaging for Detecting a Foodborne Pathogen: Campylobacter

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper is concerned with the development of a hyperspectral reflectance imaging technique for detecting and identifying one of the most common foodborne pathogens, Campylobacter. Direct plating using agars is an effective tool for laboratory tests and analyses of microorganisms. The morphology (...

  11. Classification of fecal contamination on leafy greens by hyperspectral imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral fluorescence imaging system was developed and used to obtain several two-waveband spectral ratios on leafy green vegetables, represented by romaine lettuce and baby spinach in this study. The ratios were analyzed to determine the proper one for detecting bovine fecal contamination on...

  12. Hyperspectral Imaging of Forest Resources: The Malaysian Experience

    NASA Astrophysics Data System (ADS)

    Mohd Hasmadi, I.; Kamaruzaman, J.

    2008-08-01

    Remote sensing using satellite and aircraft images are well established technology. Remote sensing application of hyperspectral imaging, however, is relatively new to Malaysian forestry. Through a wide range of wavelengths hyperspectral data are precisely capable to capture narrow bands of spectra. Airborne sensors typically offer greatly enhanced spatial and spectral resolution over their satellite counterparts, and able to control experimental design closely during image acquisition. The first study using hyperspectral imaging for forest inventory in Malaysia were conducted by Professor Hj. Kamaruzaman from the Faculty of Forestry, Universiti Putra Malaysia in 2002 using the AISA sensor manufactured by Specim Ltd, Finland. The main objective has been to develop methods that are directly suited for practical tropical forestry application at the high level of accuracy. Forest inventory and tree classification including development of single spectral signatures have been the most important interest at the current practices. Experiences from the studies showed that retrieval of timber volume and tree discrimination using this system is well and some or rather is better than other remote sensing methods. This article reviews the research and application of airborne hyperspectral remote sensing for forest survey and assessment in Malaysia.

  13. Recent Advances in Techniques for Hyperspectral Image Processing

    NASA Technical Reports Server (NTRS)

    Plaza, Antonio; Benediktsson, Jon Atli; Boardman, Joseph W.; Brazile, Jason; Bruzzone, Lorenzo; Camps-Valls, Gustavo; Chanussot, Jocelyn; Fauvel, Mathieu; Gamba, Paolo; Gualtieri, Anthony; Marconcini, Mattia; Tilton, James C.; Trianni, Giovanna

    2009-01-01

    Imaging spectroscopy, also known as hyperspectral imaging, has been transformed in less than 30 years from being a sparse research tool into a commodity product available to a broad user community. Currently, there is a need for standardized data processing techniques able to take into account the special properties of hyperspectral data. In this paper, we provide a seminal view on recent advances in techniques for hyperspectral image processing. Our main focus is on the design of techniques able to deal with the highdimensional nature of the data, and to integrate the spatial and spectral information. Performance of the discussed techniques is evaluated in different analysis scenarios. To satisfy time-critical constraints in specific applications, we also develop efficient parallel implementations of some of the discussed algorithms. Combined, these parts provide an excellent snapshot of the state-of-the-art in those areas, and offer a thoughtful perspective on future potentials and emerging challenges in the design of robust hyperspectral imaging algorithms

  14. Classification of Fecal Contamination on Leafy Greens by Hyperspectral Imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral fluorescence imaging system was developed and used to obtain several two-waveband spectral ratios on leafy green vegetables, represented by romaine lettuce and baby spinach in this study. The ratios were analyzed to determine the proper one for detecting bovine fecal contamination on...

  15. Hyperspectral Imaging for Detecting Pathogens Grown on Agar Plates

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper is concerned with the development of a hyperspectral imaging technique for detecting and identifying one of the most common foodborne pathogens, Campylobacter. Direct plating using agars is an effective tool for laboratory tests and analyses of microorganisms. The morphology (size, growth...

  16. Evaluation of physiological status of potato tubers using hyperspectral imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Visible and near-infrared hyperspectral reflectance imaging was evaluated as a rapid technique to predict the glucose and sucrose percentages in two common fresh use and chipping potato cultivars. Tubers were sampled in the 2009 season and held in multiple storage temperatures in attempt to develop ...

  17. Visible Hyperspectral Imaging for Standoff Detection of Explosives on Surfaces

    SciTech Connect

    Bernacki, Bruce E.; Blake, Thomas A.; Mendoza, Albert; Johnson, Timothy J.

    2010-11-01

    There is an ever-increasing need to be able to detect the presence of explosives, preferably from standoff distances. This paper presents an application of visible hyperspectral imaging using anomaly, polarization and spectral identification approaches for the standoff detection (13 meters) of nitroaromatic explosives on realistic painted surfaces based upon the colorimetric differences between tetryl and TNT which are enhanced by solar irradiation.

  18. Hyperspectral Imaging Technologies for Nondestructive Agro-Food Evaluation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Over the past decade, researchers at the Agricultural Research Service (ARS), United States Department of Agriculture (USDA), have developed several versions of line-scan-based hyperspectral imaging systems capable of both visible to near-infrared reflectance and fluorescence methods. These line-s...

  19. Hyperspectral imaging in medicine: image pre-processing problems and solutions in Matlab.

    PubMed

    Koprowski, Robert

    2015-11-01

    The paper presents problems and solutions related to hyperspectral image pre-processing. New methods of preliminary image analysis are proposed. The paper shows problems occurring in Matlab when trying to analyse this type of images. Moreover, new methods are discussed which provide the source code in Matlab that can be used in practice without any licensing restrictions. The proposed application and sample result of hyperspectral image analysis. PMID:25676816

  20. [Design of hyperspectral imaging system based on LCTF].

    PubMed

    Zhang, Dong-ying; Hong, Jin; Tang, Wei-ping; Yang, Wei-feng; Luo, Jun; Qiao, Yan-li; Zhang, Xie

    2008-10-01

    A new compact lightweight imaging system for hyperspectral imaging is described. The system can be thought of as the substitute for traditional mechanical filter-wheel sensor. The system is based on different techniques. It uses an electronic controlled LCTF(liquid crystal tunable filter) which provided rapid and vibrationless selection of any wavelength in the visible to IR range. The imaging system consisted of an optic lens, a CRI VariSpec LCTF and a Dalsa 1M30 camera. First the outline of this system setup is presented, then the optics designed is introduced, next the working principle of LCTF is described in details. A field experiment with the imaging system loaded on an airship was carried out and collected hyperspectral solid image. The images obtained had higher spectral and spatial resolution. Some parts of the 540-600 nm components of the 16-band image cube were also shown. Finally, the data acquired were rough processed to get reflection spectrum(from 420 to 720 nm) of three targets. It is concluded that the experiment has proved that the imaging system is effective in obtaining hyperspectral data. The image captured by the system can be applied to spectral estimation, spectra based classification and spectral based analysis. PMID:19123429

  1. Identification of inflammation sites in arthritic joints using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Paluchowski, Lukasz A.; Milanic, Matija; Bjorgan, Asgeir; Grandaunet, Berit; Dhainaut, Alvilde; Hoff, Mari; Randeberg, Lise L.

    2014-03-01

    Inflammatory arthritic diseases have prevalence between 2 and 3% and may lead to joint destruction and deformation resulting in a loss of function. Patient's quality of life is often severely affected as the disease attacks hands and finger joints. Pathology involved in arthritis includes angiogenesis, hyper-vascularization, hyper-metabolism and relative hypoxia. We have employed hyperspectral imaging to study the hemodynamics of affected- and non-affected joints and tissue. Two hyperspectral, push-broom cameras were used (VNIR-1600, SWIR-320i, Norsk Elektro Optikk AS, Norway). Optical spectra (400nm - 1700nm) of high spectral resolution were collected from 15 patients with visible symptoms of arthritic rheumatic diseases in at least one joint. The control group consisted of 10 healthy individuals. Concentrations of dominant chromophores were calculated based on analytical calculations of light transport in tissue. Image processing was used to analyze hyperspectral data and retrieve information, e.g. blood concentration and tissue oxygenation maps. The obtained results indicate that hyperspectral imaging can be used to quantify changes within affected joints and surrounding tissue. Further improvement of this method will have positive impact on diagnosis of arthritic joints at an early stage. Moreover it will enable development of fast, noninvasive and noncontact diagnostic tool of arthritic joints

  2. Analysis of hyperspectral scattering images using a moment method for apple firmness prediction

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This article reports on using a moment method to extract features from the hyperspectral scattering profiles for apple fruit firmness prediction. Hyperspectral scattering images between 500 nm and 1000 nm were acquired online, using a hyperspectral scattering system, for ‘Golden Delicious’, ’Jonagol...

  3. Hyperspectral image reconstruction using RGB color for foodborne pathogen detection on agar plates

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper reports the latest development of a color vision technique for detecting colonies of foodborne pathogens grown on agar plates with a hyperspectral image classification model that was developed using full hyperspectral data. The hyperspectral classification model depended on reflectance sp...

  4. Detecting citrus canker by hyperspectral reflectance imaging and PCA-based image classification method

    NASA Astrophysics Data System (ADS)

    Qin, Jianwei; Burks, Thomas F.; Kim, Moon S.; Chao, Kuanglin; Ritenour, Mark A.

    2008-04-01

    Citrus canker is one of the most devastating diseases that threaten citrus crops. Technologies that can efficiently identify citrus canker would assure fruit quality and safety and enhance the competitiveness and profitability of the citrus industry. This research was aimed to investigate the potential of using hyperspectral imaging technique for detecting canker lesions on citrus fruit. A portable hyperspectral imaging system consisting of an automatic sample handling unit, a light source, and a hyperspectral imaging unit was developed for citrus canker detection. The imaging system was used to acquire reflectance images from citrus samples in the wavelength range between 400 nm and 900 nm. Ruby Red grapefruits with normal and various diseased skin conditions including canker, copper burn, greasy spot, wind scar, cake melanose, and specular melanose were tested. Hyperspectral reflectance images were analyzed using principal component analysis (PCA) to compress the 3-D hyperspectral image data and extract useful image features that could be used to discriminate cankerous samples from normal and other diseased samples. Image processing and classification algorithms were developed based upon the transformed images of PCA. The overall accuracy for canker detection was 92.7%. This research demonstrated that hyperspectral imaging technique could be used for discriminating citrus canker from other confounding diseases.

  5. A hyperspectral image analysis workbench for environmental science applications

    SciTech Connect

    Christiansen, J.H.; Zawada, D.G.; Simunich, K.L.; Slater, J.C.

    1992-10-01

    A significant challenge to the information sciences is to provide more powerful and accessible means to exploit the enormous wealth of data available from high-resolution imaging spectrometry, or ``hyperspectral`` imagery, for analysis, for mapping purposes, and for input to environmental modeling applications. As an initial response to this challenge, Argonne`s Advanced Computer Applications Center has developed a workstation-based prototype software workbench which employs Al techniques and other advanced approaches to deduce surface characteristics and extract features from the hyperspectral images. Among its current capabilities, the prototype system can classify pixels by abstract surface type. The classification process employs neural network analysis of inputs which include pixel spectra and a variety of processed image metrics, including image ``texture spectra`` derived from fractal signatures computed for subimage tiles at each wavelength.

  6. [Molecular hyperspectral imaging (MHSI) system and application in biochemical medicine].

    PubMed

    Liu, Hong-Ying; Li, Qing-Li; Wang, Yi-Ting; Liu, Jin-Gao; Xue, Yong-Qi

    2011-10-01

    A novel molecular hyperspectral imaging (MHSI) system based on AOTF (acousto-optic tunable filters) was presented. The system consists of microscope, AOTF-based spectrometer, matrix CCD, image collection card and computer. The spectral range of the MHSI is from 550 to 1 000 nm. The spectral resolution is less than 2 nm, and the spatial resolution is about 0.3 microm. This paper has also presented that spectral curves extracted from the corrected hyperspectral data of the sample, which have been preprocessed by the gray correction coefficient, can more truly represent biochemical characteristic of the sample. The system can supply not only single band images in the visible range, but also spectrum curve of random pixel of sample image. This system can be widely used in various fields of biomedicine, clinical medicine, material science and microelectronics. PMID:22250515

  7. A hyperspectral image analysis workbench for environmental science applications

    SciTech Connect

    Christiansen, J.H.; Zawada, D.G.; Simunich, K.L.; Slater, J.C.

    1992-01-01

    A significant challenge to the information sciences is to provide more powerful and accessible means to exploit the enormous wealth of data available from high-resolution imaging spectrometry, or hyperspectral'' imagery, for analysis, for mapping purposes, and for input to environmental modeling applications. As an initial response to this challenge, Argonne's Advanced Computer Applications Center has developed a workstation-based prototype software workbench which employs Al techniques and other advanced approaches to deduce surface characteristics and extract features from the hyperspectral images. Among its current capabilities, the prototype system can classify pixels by abstract surface type. The classification process employs neural network analysis of inputs which include pixel spectra and a variety of processed image metrics, including image texture spectra'' derived from fractal signatures computed for subimage tiles at each wavelength.

  8. Hyperspectral imaging for non-contact analysis of forensic traces.

    PubMed

    Edelman, G J; Gaston, E; van Leeuwen, T G; Cullen, P J; Aalders, M C G

    2012-11-30

    Hyperspectral imaging (HSI) integrates conventional imaging and spectroscopy, to obtain both spatial and spectral information from a specimen. This technique enables investigators to analyze the chemical composition of traces and simultaneously visualize their spatial distribution. HSI offers significant potential for the detection, visualization, identification and age estimation of forensic traces. The rapid, non-destructive and non-contact features of HSI mark its suitability as an analytical tool for forensic science. This paper provides an overview of the principles, instrumentation and analytical techniques involved in hyperspectral imaging. We describe recent advances in HSI technology motivating forensic science applications, e.g. the development of portable and fast image acquisition systems. Reported forensic science applications are reviewed. Challenges are addressed, such as the analysis of traces on backgrounds encountered in casework, concluded by a summary of possible future applications. PMID:23088824

  9. Towards real-time medical diagnostics using hyperspectral imaging technology

    NASA Astrophysics Data System (ADS)

    Bjorgan, Asgeir; Randeberg, Lise L.

    2015-07-01

    Hyperspectral imaging provides non-contact, high resolution spectral images which has a substantial diagnostic potential. This can be used for e.g. diagnosis and early detection of arthritis in finger joints. Processing speed is currently a limitation for clinical use of the technique. A real-time system for analysis and visualization using GPU processing and threaded CPU processing is presented. Images showing blood oxygenation, blood volume fraction and vessel enhanced images are among the data calculated in real-time. This study shows the potential of real-time processing in this context. A combination of the processing modules will be used in detection of arthritic finger joints from hyperspectral reflectance and transmittance data.

  10. Hyperspectral image visualization using t-distributed stochastic neighbor embedding

    NASA Astrophysics Data System (ADS)

    Zhang, Biyin; Yu, Xin

    2015-12-01

    Hyperspectral image visualization reduces high-dimensional spectral bands to three color channels, which are sought in order to explain well the nonlinear data characteristics that are hidden in the high-dimensional spectral bands. Despite the surge in the linear visualization techniques, the development of nonlinear visualization has been limited. The paper presents a new technique for visualization of hyperspectral image using t-distributed stochastic neighbor embedding, called VHI-tSNE, which learns a nonlinear mapping between the high-dimensional spectral space and the three-dimensional color space. VHI-tSNE transforms hyperspectral data into bilateral probability similarities, and employs a heavy-tailed distribution in three-dimensional color space to alleviate the crowding problem and optimization problem in SNE technique. We evaluate the performance of VHI-tSNE in experiments on several hyperspectral imageries, in which we compare it to the performance of other state-of-art techniques. The results of experiments demonstrated the strength of the proposed technique.

  11. Damage and quality assessment in wheat by NIR hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Delwiche, Stephen R.; Kim, Moon S.; Dong, Yanhong

    2010-04-01

    Fusarium head blight is a fungal disease that affects the world's small grains, such as wheat and barley. Attacking the spikelets during development, the fungus causes a reduction of yield and grain of poorer processing quality. It also is a health concern because of the secondary metabolite, deoxynivalenol, which often accompanies the fungus. While chemical methods exist to measure the concentration of the mycotoxin and manual visual inspection is used to ascertain the level of Fusarium damage, research has been active in developing fast, optically based techniques that can assess this form of damage. In the current study a near-infrared (1000-1700 nm) hyperspectral image system was assembled and applied to Fusarium-damaged kernel recognition. With anticipation of an eventual multispectral imaging system design, 5 wavelengths were manually selected from a pool of 146 images as the most promising, such that when combined in pairs or triplets, Fusarium damage could be identified. We present the results of two pairs of wavelengths [(1199, 1474 nm) and (1315, 1474 nm)] whose reflectance values produced adequate separation of kernels of healthy appearance (i.e., asymptomatic condition) from kernels possessing Fusarium damage.

  12. Hyperspectral imaging based biomass and nitrogen content estimations from light-weight UAV

    NASA Astrophysics Data System (ADS)

    Pölönen, I.; Saari, H.; Kaivosoja, J.; Honkavaara, E.; Pesonen, L.

    2013-10-01

    Hyperspectral imaging based precise fertilization is challenge in the northern Europe, because of the cloud conditions. In this paper we will introduce schemes for the biomass and nitrogen content estimations from hyperspectral images. In this research we used the Fabry-Perot interferometer based hypespectral imager that enables hyperspectral imaging from lightweight UAVs. During the summers 2011 and 2012 imaging and flight campaigns were carried out on the Finnish test field. Estimation mehtod uses features from linear and non-linear unmixing and vegetation indices. The results showed that the concept of small hyperspectral imager, UAV and data analysis is ready to operational use.

  13. Detection of Lettuce Discoloration Using Hyperspectral Reflectance Imaging.

    PubMed

    Mo, Changyeun; Kim, Giyoung; Lim, Jongguk; Kim, Moon S; Cho, Hyunjeong; Cho, Byoung-Kwan

    2015-01-01

    Rapid visible/near-infrared (VNIR) hyperspectral imaging methods, employing both a single waveband algorithm and multi-spectral algorithms, were developed in order to discrimination between sound and discolored lettuce. Reflectance spectra for sound and discolored lettuce surfaces were extracted from hyperspectral reflectance images obtained in the 400-1000 nm wavelength range. The optimal wavebands for discriminating between discolored and sound lettuce surfaces were determined using one-way analysis of variance. Multi-spectral imaging algorithms developed using ratio and subtraction functions resulted in enhanced classification accuracy of above 99.9% for discolored and sound areas on both adaxial and abaxial lettuce surfaces. Ratio imaging (RI) and subtraction imaging (SI) algorithms at wavelengths of 552/701 nm and 557-701 nm, respectively, exhibited better classification performances compared to results obtained for all possible two-waveband combinations. These results suggest that hyperspectral reflectance imaging techniques can potentially be used to discriminate between discolored and sound fresh-cut lettuce. PMID:26610510

  14. Detection of Lettuce Discoloration Using Hyperspectral Reflectance Imaging

    PubMed Central

    Mo, Changyeun; Kim, Giyoung; Lim, Jongguk; Kim, Moon S.; Cho, Hyunjeong; Cho, Byoung-Kwan

    2015-01-01

    Rapid visible/near-infrared (VNIR) hyperspectral imaging methods, employing both a single waveband algorithm and multi-spectral algorithms, were developed in order to discrimination between sound and discolored lettuce. Reflectance spectra for sound and discolored lettuce surfaces were extracted from hyperspectral reflectance images obtained in the 400–1000 nm wavelength range. The optimal wavebands for discriminating between discolored and sound lettuce surfaces were determined using one-way analysis of variance. Multi-spectral imaging algorithms developed using ratio and subtraction functions resulted in enhanced classification accuracy of above 99.9% for discolored and sound areas on both adaxial and abaxial lettuce surfaces. Ratio imaging (RI) and subtraction imaging (SI) algorithms at wavelengths of 552/701 nm and 557–701 nm, respectively, exhibited better classification performances compared to results obtained for all possible two-waveband combinations. These results suggest that hyperspectral reflectance imaging techniques can potentially be used to discriminate between discolored and sound fresh-cut lettuce. PMID:26610510

  15. Hyperspectral imaging of bruises in the SWIR spectral region

    NASA Astrophysics Data System (ADS)

    Randeberg, Lise L.; Hernandez-Palacios, Julio

    2012-02-01

    Optical diagnostics of bruised skin might provide important information for characterization and age determination of such injuries. Hyperspectral imaging is one of the optical techniques that have been employed for bruise characterization. This technique combines high spatial and spectral resolution and makes it possible to study both chromophore signatures and -distributions in an injury. Imaging and spectroscopy in the visible spectral range have resulted in increased knowledge about skin bruises. So far the SWIR region has not been explored for this application. The main objective of the current study was to characterize bruises in the SWIR wavelength range. Hyperspectral images in the SWIR (950-2500nm ) and VNIR (400-850nm) spectral range were collected from 3 adult volunteers with bruises of known age. Data were collected over a period of 8 days. The data were analyzed using spectroscopic techniques and statistical image analysis. Preliminary results from the pilot study indicate that SWIR hyperspectral imaging might be an important supplement to imaging in the visible part of the spectrum. The technique emphasizes local edema and gives a possibility to visualize features that cannot easily be seen in the visible part of the spectrum.

  16. Hyperspectral image-based methods for spectral diversity

    NASA Astrophysics Data System (ADS)

    Sotomayor, Alejandro; Medina, Ollantay; Chinea, J. D.; Manian, Vidya

    2015-05-01

    Hyperspectral images are an important tool to assess ecosystem biodiversity. To obtain more precise analysis of biodiversity indicators that agree with indicators obtained using field data, analysis of spectral diversity calculated from images have to be validated with field based diversity estimates. The plant species richness is one of the most important indicators of biodiversity. This indicator can be measured in hyperspectral images considering the Spectral Variation Hypothesis (SVH) which states that the spectral heterogeneity is related to spatial heterogeneity and thus to species richness. The goal of this research is to capture spectral heterogeneity from hyperspectral images for a terrestrial neo tropical forest site using Vector Quantization (VQ) method and then use the result for prediction of plant species richness. The results are compared with that of Hierarchical Agglomerative Clustering (HAC). The validation of the process index is done calculating the Pearson correlation coefficient between the Shannon entropy from actual field data and the Shannon entropy computed in the images. One of the advantages of developing more accurate analysis tools would be the extension of the analysis to larger zones. Multispectral image with a lower spatial resolution has been evaluated as a prospective tool for spectral diversity.

  17. Hyperspectral laser-induced autofluorescence imaging of dental caries

    NASA Astrophysics Data System (ADS)

    Bürmen, Miran; Fidler, Aleš; Pernuš, Franjo; Likar, Boštjan

    2012-01-01

    Dental caries is a disease characterized by demineralization of enamel crystals leading to the penetration of bacteria into the dentine and pulp. Early detection of enamel demineralization resulting in increased enamel porosity, commonly known as white spots, is a difficult diagnostic task. Laser induced autofluorescence was shown to be a useful method for early detection of demineralization. The existing studies involved either a single point spectroscopic measurements or imaging at a single spectral band. In the case of spectroscopic measurements, very little or no spatial information is acquired and the measured autofluorescence signal strongly depends on the position and orientation of the probe. On the other hand, single-band spectral imaging can be substantially affected by local spectral artefacts. Such effects can significantly interfere with automated methods for detection of early caries lesions. In contrast, hyperspectral imaging effectively combines the spatial information of imaging methods with the spectral information of spectroscopic methods providing excellent basis for development of robust and reliable algorithms for automated classification and analysis of hard dental tissues. In this paper, we employ 405 nm laser excitation of natural caries lesions. The fluorescence signal is acquired by a state-of-the-art hyperspectral imaging system consisting of a high-resolution acousto-optic tunable filter (AOTF) and a highly sensitive Scientific CMOS camera in the spectral range from 550 nm to 800 nm. The results are compared to the contrast obtained by near-infrared hyperspectral imaging technique employed in the existing studies on early detection of dental caries.

  18. Study on the Methods of Detecting Cucumber Downy Mildew Using Hyperspectral Imaging Technology

    NASA Astrophysics Data System (ADS)

    Tian, Youwen; Zhang, Lin

    Hyperspectral imaging technology, which can integrate the advantages of spectral detection and image detection, meets the need of detecting the cucumber diseases fast and nondestructively. In this paper, hyperspectral imaging technology is adopted to detect the cucumber downy mildew fast and nondestructively. Firstly, hyperspectral images of cucumber leaves infected downy mildew are acquired by the hyperspectral image acquisition system. And optimum wavelengths are collected by the principal component analysis to get the featured images. Then the image fusion technology is adopted to combine collected images with the featured images to form new images by pixel-level image fusion. Finally, the methods of the image enhancement, binarization, corrosion and dilatation treatments are carried out, so the cucumber downy mildew is detected. The result shows that the accuracy rate of the algorithm for detecting cucumber disease can reach nearly 90%. Studies have shown that hyperspectral imaging technology can be used to detect cucumber downy mildew.

  19. Manifold alignment for classification of multitemporal hyperspectral image data

    NASA Astrophysics Data System (ADS)

    Yang, Hsiu-Han

    Analyzing remotely sensed images to obtain land cover classification maps is an effective approach for acquiring information over landscapes that can be accomplished over extended areas with limited ground surveys. Further, with advances in remote sensing technology, spaceborne hyperspectral sensors provide the capability to acquire a set of images that have both high spectral and temporal resolution. These images are suitable for monitoring and analyzing environmental changes with subtle spectral characteristics. However, inherent characteristics of multitemporal hyperspectral images, including high dimensionality, nonlinearity, and nonstationarity phenomena over time and across large areas, pose several challenges for classification. This research addresses the issues of classification tasks in the presence of spectral shifts within multitemporal hyperspectral images by leveraging the concept of the data manifold. Although manifold learning has been applied successfully in single image hyperspectral data classification to address high dimensionality and nonlinear spectral responses, research related to manifold learning for multitemporal classification studies is limited. The proposed approaches utilize spectral signatures and spatial proximity to construct similar "local" geometries of temporal images. By aligning these underlying manifolds optimally, the impacts of nonstationary effects are mitigated and classification is accomplished in a representative temporal data manifold. "Global" manifolds learned from temporal hyperspectral images have a major advantage in faithful representation of the data in an image, such as retaining relationships between different classes. Local manifolds are favored in discriminating difficult classes and for computation efficiency. A new hybrid global-local manifold alignment method that combines the advantages of global and local manifolds for effective multitemporal image classification is also proposed. Results illustrate the

  20. Hyperspectral image reconstruction using RGB color for foodborne pathogen detection on agar plates

    NASA Astrophysics Data System (ADS)

    Yoon, Seung-Chul; Shin, Tae-Sung; Park, Bosoon; Lawrence, Kurt C.; Heitschmidt, Gerald W.

    2014-03-01

    This paper reports the latest development of a color vision technique for detecting colonies of foodborne pathogens grown on agar plates with a hyperspectral image classification model that was developed using full hyperspectral data. The hyperspectral classification model depended on reflectance spectra measured in the visible and near-infrared spectral range from 400 and 1,000 nm (473 narrow spectral bands). Multivariate regression methods were used to estimate and predict hyperspectral data from RGB color values. The six representative non-O157 Shiga-toxin producing Eschetichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) were grown on Rainbow agar plates. A line-scan pushbroom hyperspectral image sensor was used to scan 36 agar plates grown with pure STEC colonies at each plate. The 36 hyperspectral images of the agar plates were divided in half to create training and test sets. The mean Rsquared value for hyperspectral image estimation was about 0.98 in the spectral range between 400 and 700 nm for linear, quadratic and cubic polynomial regression models and the detection accuracy of the hyperspectral image classification model with the principal component analysis and k-nearest neighbors for the test set was up to 92% (99% with the original hyperspectral images). Thus, the results of the study suggested that color-based detection may be viable as a multispectral imaging solution without much loss of prediction accuracy compared to hyperspectral imaging.

  1. An optimized hybrid encode based compression algorithm for hyperspectral image

    NASA Astrophysics Data System (ADS)

    Wang, Cheng; Miao, Zhuang; Feng, Weiyi; He, Weiji; Chen, Qian; Gu, Guohua

    2013-12-01

    Compression is a kernel procedure in hyperspectral image processing due to its massive data which will bring great difficulty in date storage and transmission. In this paper, a novel hyperspectral compression algorithm based on hybrid encoding which combines with the methods of the band optimized grouping and the wavelet transform is proposed. Given the characteristic of correlation coefficients between adjacent spectral bands, an optimized band grouping and reference frame selection method is first utilized to group bands adaptively. Then according to the band number of each group, the redundancy in the spatial and spectral domain is removed through the spatial domain entropy coding and the minimum residual based linear prediction method. Thus, embedded code streams are obtained by encoding the residual images using the improved embedded zerotree wavelet based SPIHT encode method. In the experments, hyperspectral images collected by the Airborne Visible/ Infrared Imaging Spectrometer (AVIRIS) were used to validate the performance of the proposed algorithm. The results show that the proposed approach achieves a good performance in reconstructed image quality and computation complexity.The average peak signal to noise ratio (PSNR) is increased by 0.21~0.81dB compared with other off-the-shelf algorithms under the same compression ratio.

  2. Hyperspectral imaging for detection of cholesterol in human skin

    NASA Astrophysics Data System (ADS)

    Milanič, Matija; Bjorgan, Asgeir; Larsson, Marcus; Marraccini, Paolo; Strömberg, Tomas; Randeberg, Lise L.

    2015-03-01

    Hypercholesterolemia is characterized by high levels of cholesterol in the blood and is associated with an increased risk of atherosclerosis and coronary heart disease. Early detection of hypercholesterolemia is necessary to prevent onset and progress of cardiovascular disease. Optical imaging techniques might have a potential for early diagnosis and monitoring of hypercholesterolemia. In this study, hyperspectral imaging was investigated for this application. The main aim of the study was to identify spectral and spatial characteristics that can aid identification of hypercholesterolemia in facial skin. The first part of the study involved a numerical simulation of human skin affected by hypercholesterolemia. A literature survey was performed to identify characteristic morphological and physiological parameters. Realistic models were prepared and Monte Carlo simulations were performed to obtain hyperspectral images. Based on the simulations optimal wavelength regions for differentiation between normal and cholesterol rich skin were identified. Minimum Noise Fraction transformation (MNF) was used for analysis. In the second part of the study, the simulations were verified by a clinical study involving volunteers with elevated and normal levels of cholesterol. The faces of the volunteers were scanned by a hyperspectral camera covering the spectral range between 400 nm and 720 nm, and characteristic spectral features of the affected skin were identified. Processing of the images was done after conversion to reflectance and masking of the images. The identified features were compared to the known cholesterol levels of the subjects. The results of this study demonstrate that hyperspectral imaging of facial skin can be a promising, rapid modality for detection of hypercholesterolemia.

  3. Recent developments in hyperspectral imaging for assessment of food quality and safety.

    PubMed

    Huang, Hui; Liu, Li; Ngadi, Michael O

    2014-01-01

    Hyperspectral imaging which combines imaging and spectroscopic technology is rapidly gaining ground as a non-destructive, real-time detection tool for food quality and safety assessment. Hyperspectral imaging could be used to simultaneously obtain large amounts of spatial and spectral information on the objects being studied. This paper provides a comprehensive review on the recent development of hyperspectral imaging applications in food and food products. The potential and future work of hyperspectral imaging for food quality and safety control is also discussed. PMID:24759119

  4. Recent Developments in Hyperspectral Imaging for Assessment of Food Quality and Safety

    PubMed Central

    Huang, Hui; Liu, Li; Ngadi, Michael O.

    2014-01-01

    Hyperspectral imaging which combines imaging and spectroscopic technology is rapidly gaining ground as a non-destructive, real-time detection tool for food quality and safety assessment. Hyperspectral imaging could be used to simultaneously obtain large amounts of spatial and spectral information on the objects being studied. This paper provides a comprehensive review on the recent development of hyperspectral imaging applications in food and food products. The potential and future work of hyperspectral imaging for food quality and safety control is also discussed. PMID:24759119

  5. A minimum spanning forest based hyperspectral image classification method for cancerous tissue detection

    NASA Astrophysics Data System (ADS)

    Pike, Robert; Patton, Samuel K.; Lu, Guolan; Halig, Luma V.; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

    2014-03-01

    Hyperspectral imaging is a developing modality for cancer detection. The rich information associated with hyperspectral images allow for the examination between cancerous and healthy tissue. This study focuses on a new method that incorporates support vector machines into a minimum spanning forest algorithm for differentiating cancerous tissue from normal tissue. Spectral information was gathered to test the algorithm. Animal experiments were performed and hyperspectral images were acquired from tumor-bearing mice. In vivo imaging experimental results demonstrate the applicability of the proposed classification method for cancer tissue classification on hyperspectral images.

  6. [Decomposition of Interference Hyperspectral Images Using Improved Morphological Component Analysis].

    PubMed

    Wen, Jia; Zhao, Jun-suo; Wang, Cai-ling; Xia, Yu-li

    2016-01-01

    As the special imaging principle of the interference hyperspectral image data, there are lots of vertical interference stripes in every frames. The stripes' positions are fixed, and their pixel values are very high. Horizontal displacements also exist in the background between the frames. This special characteristics will destroy the regular structure of the original interference hyperspectral image data, which will also lead to the direct application of compressive sensing theory and traditional compression algorithms can't get the ideal effect. As the interference stripes signals and the background signals have different characteristics themselves, the orthogonal bases which can sparse represent them will also be different. According to this thought, in this paper the morphological component analysis (MCA) is adopted to separate the interference stripes signals and background signals. As the huge amount of interference hyperspectral image will lead to glow iterative convergence speed and low computational efficiency of the traditional MCA algorithm, an improved MCA algorithm is also proposed according to the characteristics of the interference hyperspectral image data, the conditions of iterative convergence is improved, the iteration will be terminated when the error of the separated image signals and the original image signals are almost unchanged. And according to the thought that the orthogonal basis can sparse represent the corresponding signals but cannot sparse represent other signals, an adaptive update mode of the threshold is also proposed in order to accelerate the computational speed of the traditional MCA algorithm, in the proposed algorithm, the projected coefficients of image signals at the different orthogonal bases are calculated and compared in order to get the minimum value and the maximum value of threshold, and the average value of them is chosen as an optimal threshold value for the adaptive update mode. The experimental results prove that

  7. Correction of axial optical aberrations in hyperspectral imaging systems

    NASA Astrophysics Data System (ADS)

    Špiclin, Žiga; Pernuš, Franjo; Likar, Boštjan

    2011-03-01

    In hyper-spectral imaging systems with a wide spectral range, axial optical aberrations may lead to a significant blurring of image intensities in certain parts of the spectral range. Axial optical aberrations arise from the indexof- refraction variations that is dependent on the wavelength of incident light. To correct axial optical aberrations the point-spread function (PSF) of the image acquisition system needs to be identified. We proposed a multiframe joint blur identification and image restoration method that maximizes the likelihood of local image energy distributions between spectral images. Gaussian mixture model based density estimate provides a link between corresponding spatial information shared among spectral images so as to find and restore the image edges via a PSF update. Model of the PSF was assumed to be a linear combination of Gaussian functions, therefore the blur identification process had to find only the corresponding scalar weights of each Gaussian function. Using the identified PSF, image restoration was performed by the iterative Richardson-Lucy algorithm. Experiments were conducted on four different biological samples using a hyper-spectral imaging system based on acousto-optic tunable filter in the visible spectral range (0.55 - 1.0 μm). By running the proposed method, the quality of raw spectral images was substantially improved. Image quality improvements were quantified by a measure of contrast and demonstrate the potential of the proposed method for the correction of axial optical aberrations.

  8. The challenges of analysing blood stains with hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Kuula, J.; Puupponen, H.-H.; Rinta, H.; Pölönen, I.

    2014-06-01

    Hyperspectral imaging is a potential noninvasive technology for detecting, separating and identifying various substances. In the forensic and military medicine and other CBRNE related use it could be a potential method for analyzing blood and for scanning other human based fluids. For example, it would be valuable to easily detect whether some traces of blood are from one or more persons or if there are some irrelevant substances or anomalies in the blood. This article represents an experiment of separating four persons' blood stains on a white cotton fabric with a SWIR hyperspectral camera and FT-NIR spectrometer. Each tested sample includes standardized 75 _l of 100 % blood. The results suggest that on the basis of the amount of erythrocytes in the blood, different people's blood might be separable by hyperspectral analysis. And, referring to the indication given by erythrocytes, there might be a possibility to find some other traces in the blood as well. However, these assumptions need to be verified with wider tests, as the number of samples in the study was small. According to the study there also seems to be several biological, chemical and physical factors which affect alone and together on the hyperspectral analyzing results of blood on fabric textures, and these factors need to be considered before making any further conclusions on the analysis of blood on various materials.

  9. Airborne Hyperspectral Imaging of Seagrass and Coral Reef

    NASA Astrophysics Data System (ADS)

    Merrill, J.; Pan, Z.; Mewes, T.; Herwitz, S.

    2013-12-01

    This talk presents the process of project preparation, airborne data collection, data pre-processing and comparative analysis of a series of airborne hyperspectral projects focused on the mapping of seagrass and coral reef communities in the Florida Keys. As part of a series of large collaborative projects funded by the NASA ROSES program and the Florida Fish and Wildlife Conservation Commission and administered by the NASA UAV Collaborative, a series of airborne hyperspectral datasets were collected over six sites in the Florida Keys in May 2012, October 2012 and May 2013 by Galileo Group, Inc. using a manned Cessna 172 and NASA's SIERRA Unmanned Aerial Vehicle. Precise solar and tidal data were used to calculate airborne collection parameters and develop flight plans designed to optimize data quality. Two independent Visible and Near-Infrared (VNIR) hyperspectral imaging systems covering 400-100nm were used to collect imagery over six Areas of Interest (AOIs). Multiple collections were performed over all sites across strict solar windows in the mornings and afternoons. Independently developed pre-processing algorithms were employed to radiometrically correct, synchronize and georectify individual flight lines which were then combined into color balanced mosaics for each Area of Interest. The use of two different hyperspectral sensor as well as environmental variations between each collection allow for the comparative analysis of data quality as well as the iterative refinement of flight planning and collection parameters.

  10. Real-time airborne hyperspectral imaging of land mines

    NASA Astrophysics Data System (ADS)

    Ivanco, Tyler; Achal, Steve; McFee, John E.; Anger, Cliff; Young, Jane

    2007-04-01

    DRDC Suffeld and Itres Research have jointly investigated the use of visible and infrared hyperspectral imaging (HSI) for surface and buried land mine detection since 1989. These studies have demonstrated reliable passive HSI detection of surface-laid mines, based on their reflectance spectra, from airborne and ground-based platforms. Commercial HSI instruments collect and store image data at aircraft speeds, but the data are analysed off- line. This is useful for humanitarian demining, but unacceptable for military countermine operations. We have developed a hardware and software system with algorithms that can process the raw hyperspectral data in real time to detect mines. The custom algorithms perform radiometric correction of the raw data, then classify pixels of the corrected data, referencing a spectral signature library. The classification results are stored and displayed in real time, that is, within a few frame times of the data acquisition. Such real-time mine detection was demonstrated for the first time from a slowly moving land vehicle in March 2000. This paper describes an improved system which can achieve real-time detection of mines from an airborne platform, with its commensurately higher data rates. The system is presently compatible with the Itres family of visible/near infrared, short wave infrared and thermal infrared pushbroom hyperspectral imagers and its broadband thermal infrared pushbroom imager. Experiments to detect mines from an airborne platform in real time were conducted at DRDC Suffield in November 2006. Surface-laid land mines were detected in real time from a slowly moving helicopter with generally good detection rates and low false alarm rates. To the authors' knowledge, this is the first time that land mines have been detected from an airborne platform in real time using hyperspectral imaging.

  11. Preprocessing and compression of Hyperspectral images captured onboard UAVs

    NASA Astrophysics Data System (ADS)

    Herrero, Rolando; Cadirola, Martin; Ingle, Vinay K.

    2015-10-01

    Advancements in image sensors and signal processing have led to the successful development of lightweight hyperspectral imaging systems that are critical to the deployment of Photometry and Remote Sensing (PaRS) capabilities in unmanned aerial vehicles (UAVs). In general, hyperspectral data cubes include a few dozens of spectral bands that are extremely useful for remote sensing applications that range from detection of land vegetation to monitoring of atmospheric products derived from the processing of lower level radiance images. Because these data cubes are captured in the challenging environment of UAVs, where resources are limited, source encoding by means of compression is a fundamental mechanism that considerably improves the overall system performance and reliability. In this paper, we focus on the hyperspectral images captured by a state-of-the-art commercial hyperspectral camera by showing the results of applying ultraspectral data compression to the obtained data set. Specifically the compression scheme that we introduce integrates two stages; (1) preprocessing and (2) compression itself. The outcomes of this procedure are linear prediction coefficients and an error signal that, when encoded, results in a compressed version of the original image. Second, preprocessing and compression algorithms are optimized and have their time complexity analyzed to guarantee their successful deployment using low power ARM based embedded processors in the context of UAVs. Lastly, we compare the proposed architecture against other well known schemes and show how the compression scheme presented in this paper outperforms all of them by providing substantial improvement and delivering both lower compression rates and lower distortion.

  12. Infrared hyperspectral upconversion imaging using spatial object translation.

    PubMed

    Kehlet, Louis Martinus; Sanders, Nicolai; Tidemand-Lichtenberg, Peter; Dam, Jeppe Seidelin; Pedersen, Christian

    2015-12-28

    In this paper hyperspectral imaging in the mid-infrared wavelength region is realised using nonlinear frequency upconversion. The infrared light is converted to the near-infrared region for detection with a Si-based CCD camera. The object is translated in a predefined grid by motorized actuators and an image is recorded for each position. A sequence of such images is post-processed into a series of monochromatic images in a wavelength range defined by the phasematch condition and numerical aperture of the upconversion system. A standard USAF resolution target and a polystyrene film are used to impart spatial and spectral information unto the source. PMID:26832059

  13. Construction and hyperspectral imaging of quantum dot lysate arrays.

    PubMed

    Rosenblatt, Kevin P; Huebschman, Michael L; Garner, Harold R

    2012-01-01

    The emerging field of proteomic molecular profiling will be driven by new technologies that can measure dozens to hundreds of proteins from a small sample input from a patient's biopsy. Lysate arrays, or reverse-phase protein microarrays, provide a platform for complex mixtures of proteins extracted from cells and tissues to be directly immobilized onto a solid support (such as a biochip with protein binding capacity) in diminutive volumes (picoliter-to-nanoliter). The proteins are spotted using precision robotics and then quantitatively assayed using primary antibodies; important posttranslational modifications, such as phosphorylations that are important for protein activation, may also be assayed to provide an estimate of the regulation of cellular signaling. Until recently, chromogenic signals and fluorescence (using organic fluorophores) detection were two strategies relied upon for signal detection. Emerging regents such as quantum dots (Qdot® nanocrystals; QD) are now employed for improved performance. QD embody a more versatile detection system because the robust signals may be time averaged and the narrow spectral emissions enable many protein targets to be quantified within the same lysate spot. Previously, we found that commercially available pegylated, streptavidin-conjugated QD were effective detection agents, with low-background affinities to spurious components within heterogeneous protein mixtures. Hyperspectral imaging allows the simultaneous detection of the different colored QD reagents within a single lysate spot. Here, we described the construction and imaging of QD lysate arrays. This technology is an emerging, enabling tool within the exciting, clinically oriented field of clinical tissue proteomics. PMID:22081354

  14. Programmable hyperspectral image mapper with on-array processing

    NASA Technical Reports Server (NTRS)

    Cutts, James A. (Inventor)

    1995-01-01

    A hyperspectral imager includes a focal plane having an array of spaced image recording pixels receiving light from a scene moving relative to the focal plane in a longitudinal direction, the recording pixels being transportable at a controllable rate in the focal plane in the longitudinal direction, an electronic shutter for adjusting an exposure time of the focal plane, whereby recording pixels in an active area of the focal plane are removed therefrom and stored upon expiration of the exposure time, an electronic spectral filter for selecting a spectral band of light received by the focal plane from the scene during each exposure time and an electronic controller connected to the focal plane, to the electronic shutter and to the electronic spectral filter for controlling (1) the controllable rate at which the recording is transported in the longitudinal direction, (2) the exposure time, and (3) the spectral band so as to record a selected portion of the scene through M spectral bands with a respective exposure time t(sub q) for each respective spectral band q.

  15. Mid-infrared hyperspectral imaging for the detection of explosive compounds

    NASA Astrophysics Data System (ADS)

    Ruxton, K.; Robertson, G.; Miller, W.; Malcolm, G. P. A.; Maker, G. T.

    2012-10-01

    Active hyperspectral imaging is a valuable tool in a wide range of applications. A developing market is the detection and identification of energetic compounds through analysis of the resulting absorption spectrum. This work presents a selection of results from a prototype mid-infrared (MWIR) hyperspectral imaging instrument that has successfully been used for compound detection at a range of standoff distances. Active hyperspectral imaging utilises a broadly tunable laser source to illuminate the scene with light over a range of wavelengths. While there are a number of illumination methods, this work illuminates the scene by raster scanning the laser beam using a pair of galvanometric mirrors. The resulting backscattered light from the scene is collected by the same mirrors and directed and focussed onto a suitable single-point detector, where the image is constructed pixel by pixel. The imaging instrument that was developed in this work is based around a MWIR optical parametric oscillator (OPO) source with broad tunability, operating at 2.6 μm to 3.7 μm. Due to material handling procedures associated with explosive compounds, experimental work was undertaken initially using simulant compounds. A second set of compounds that was tested alongside the simulant compounds is a range of confusion compounds. By having the broad wavelength tunability of the OPO, extended absorption spectra of the compounds could be obtained to aid in compound identification. The prototype imager instrument has successfully been used to record the absorption spectra for a range of compounds from the simulant and confusion sets and current work is now investigating actual explosive compounds. The authors see a very promising outlook for the MWIR hyperspectral imager. From an applications point of view this format of imaging instrument could be used for a range of standoff, improvised explosive device (IED) detection applications and potential incident scene forensic investigation.

  16. Hyperspectral imaging for detecting pathogens grown on agar plates

    NASA Astrophysics Data System (ADS)

    Yoon, Seung Chul; Lawrence, Kurt C.; Siragusa, Gregory R.; Line, John E.; Park, Bosoon; Windham, William R.

    2007-09-01

    This paper is concerned with the development of a hyperspectral imaging technique for detecting and identifying one of the most common foodborne pathogens, Campylobacter. Direct plating using agars is an effective tool for laboratory tests and analyses of microorganisms. The morphology (size, growth pattern, color, etc.) of colonies grown on agar plates has been widely used to tentatively differentiate organisms. However, it is sometimes difficult to differentiate target organisms like Campylobacters from other contaminants grown together on the same agar plates. A hyperspectral imaging system operating at the visible and near infrared (VNIR) spectral region from 400 nm to 900 nm was set up to measure spectral signatures of 17 different Campylobacter and non-Campylobacter subspecies. Protocols for culturing, imaging samples and for calibrating measured data were developed. The VNIR spectral library of all 17 organisms commonly encountered in poultry was established from calibrated hyperspectral images. A classification algorithm was developed to locate and identify Campylobacters, non-Campylobacter contaminants, and background agars with 99.29% accuracy. This research has a potential to be expanded to detect other pathogens grown on agar media.

  17. Miniaturized hyperspectral imager calibration and UAV flight campaigns

    NASA Astrophysics Data System (ADS)

    Saari, Heikki; Pölönen, Ilkka; Salo, Heikki; Honkavaara, Eija; Hakala, Teemu; Holmlund, Christer; Mäkynen, Jussi; Mannila, Rami; Antila, Tapani; Akujärvi, Altti

    2013-10-01

    VTT Technical Research Centre of Finland has developed Tunable Fabry-Perot Interferometer (FPI) based miniaturized hyperspectral imager which can be operated from light weight Unmanned Aerial Vehicles (UAV). The concept of the hyperspectral imager has been published in the SPIE Proc. 7474, 8174 and 8374. This instrument requires dedicated laboratory and on-board calibration procedures which are described. During summer 2012 extensive UAV Hyperspectral imaging campaigns in the wavelength range 400 - 900 nm at resolution range 10 - 40 nm @ FWHM were performed to study forest inventory, crop biomass and nitrogen distributions and environmental status of natural water applications. The instrument includes spectral band limiting filters which can be used for the on-board wavelength scale calibration by scanning the FPI pass band center wavelength through the low and high edge of the operational wavelength band. The procedure and results of the calibration tests will be presented. A short summary of the performed extensive UAV imaging campaign during summer 2012 will be presented.

  18. Mineral identification in hyperspectral imaging using Sparse-PCA

    NASA Astrophysics Data System (ADS)

    Yousefi, Bardia; Sojasi, Saeed; Ibarra Castanedo, Clemente; Beaudoin, Georges; Huot, François; Maldague, Xavier P. V.; Chamberland, Martin; Lalonde, Erik

    2016-05-01

    Hyperspectral imaging has been considerably developed during the recent decades. The application of hyperspectral imagery and infrared thermography, particularly for the automatic identification of minerals from satellite images, has been the subject of several interesting researches. In this study, a method is presented for the automated identification of the mineral grains typically used from satellite imagery and adapted for analyzing collected sample grains in a laboratory environment. For this, an approach involving Sparse Principle Components Analysis (SPCA) based on spectral abundance mapping techniques (i.e. SAM, SID, NormXCorr) is proposed for extraction of the representative spectral features. It develops an approximation of endmember as a reference spectrum process through the highest sparse principle component of the pure mineral grains. Subsequently, the features categorized by kernel Extreme Learning Machine (Kernel- ELM) classify and identify the mineral grains in a supervised manner. Classification is conducted in the binary scenario and the results indicate the dependency to the training spectra.

  19. Hyperspectral imaging for differentiation of foreign materials from pinto beans

    NASA Astrophysics Data System (ADS)

    Mehrubeoglu, Mehrube; Zemlan, Michael; Henry, Sam

    2015-09-01

    Food safety and quality in packaged products are paramount in the food processing industry. To ensure that packaged products are free of foreign materials, such as debris and pests, unwanted materials mixed with the targeted products must be detected before packaging. A portable hyperspectral imaging system in the visible-to-NIR range has been used to acquire hyperspectral data cubes from pinto beans that have been mixed with foreign matter. Bands and band ratios have been identified as effective features to develop a classification scheme for detection of foreign materials in pinto beans. A support vector machine has been implemented with a quadratic kernel to separate pinto beans and background (Class 1) from all other materials (Class 2) in each scene. After creating a binary classification map for the scene, further analysis of these binary images allows separation of false positives from true positives for proper removal action during packaging.

  20. Hyperspectral imaging of plasmon resonances in metallic nanoparticles.

    PubMed

    Zopf, David; Jatschka, Jacqueline; Dathe, André; Jahr, Norbert; Fritzsche, Wolfgang; Stranik, Ondrej

    2016-07-15

    The spectroscopy of metal nanoparticles shows great potential for label-free sensing. In this article we present a hyper-spectral imaging system combined with a microfluidic system, which allows full spectroscopic characterization of many individual nanoparticles simultaneously (>50 particles). With such a system we were able overcome several limitations that are present in LSPR sensing with nanoparticle ensemble. We experimentally quantified (incorporating atomic force microscopy as well) the correlation between geometry, position of plasmon resonance (λPeak) and sensitivity of the particles (Sb=1.63λPeak-812.47[nm/RIU]). We were able to follow the adsorption of protein layers and determined their spatial inhomogeneity with the help of the hyperspectral imaging. PMID:26974477

  1. Objective color classification of ecstasy tablets by hyperspectral imaging.

    PubMed

    Edelman, Gerda; Lopatka, Martin; Aalders, Maurice

    2013-07-01

    The general procedure followed in the examination of ecstasy tablets for profiling purposes includes a color description, which depends highly on the observers' perception. This study aims to provide objective quantitative color information using visible hyperspectral imaging. Both self-manufactured and illicit tablets, created with different amounts of known colorants were analyzed. We derived reflectance spectra from hyperspectral images of these tablets, and successfully determined the most likely colorant used in the production of all self-manufactured tablets and four of five illicit tablets studied. Upon classification, the concentration of the colorant was estimated using a photon propagation model and a single reference measurement of a tablet of known concentration. The estimated concentrations showed a high correlation with the actual values (R(2) = 0.9374). The achieved color information, combined with other physical and chemical characteristics, can provide a powerful tool for the comparison of tablet seizures, which may reveal their origin. PMID:23683098

  2. [Identification of Pummelo Cultivars Based on Hyperspectral Imaging Technology].

    PubMed

    Li, Xun-lan; Yi, Shi-lai; He, Shao-lan; Lü, Qiang; Xie, Rang-jin; Zheng, Yong-qiang; Deng, Lie

    2015-09-01

    Existing methods for the identification of pummelo cultivars are usually time-consuming and costly, and are therefore inconvenient to be used in cases that a rapid identification is needed. This research was aimed at identifying different pummelo cultivars by hyperspectral imaging technology which can achieve a rapid and highly sensitive measurement. A total of 240 leaf samples, 60 for each of the four cultivars were investigated. Samples were divided into two groups such as calibration set (48 samples of each cultivar) and validation set (12 samples of each cultivar) by a Kennard-Stone-based algorithm. Hyperspectral images of both adaxial and abaxial surfaces of each leaf were obtained, and were segmented into a region of interest (ROI) using a simple threshold. Spectra of leaf samples were extracted from ROI. To remove the absolute noises of the spectra, only the date of spectral range 400~1000 nm was used for analysis. Multiplicative scatter correction (MSC) and standard normal variable (SNV) were utilized for data preprocessing. Principal component analysis (PCA) was used to extract the best principal components, and successive projections algorithm (SPA) was used to extract the effective wavelengths. Least squares support vector machine (LS-SVM) was used to obtain the discrimination model of the four different pummelo cultivars. To find out the optimal values of σ2 and γ which were important parameters in LS-SVM modeling, Grid-search technique and Cross-Validation were applied. The first 10 and 11 principal components were extracted by PCA for the hyperspectral data of adaxial surface and abaxial surface, respectively. There were 31 and 21 effective wavelengths selected by SPA based on the hyperspectral data of adaxial surface and abaxial surface, respectively. The best principal components and the effective wavelengths were used as inputs of LS-SVM models, and then the PCA-LS-SVM model and the SPA-LS-SVM model were built. The results showed that 99.46% and

  3. Imaging of blood cells based on snapshot Hyper-Spectral Imaging systems

    NASA Astrophysics Data System (ADS)

    Robison, Christopher J.; Kolanko, Christopher; Bourlai, Thirimachos; Dawson, Jeremy M.

    2015-05-01

    Snapshot Hyper-Spectral imaging systems are capable of capturing several spectral bands simultaneously, offering coregistered images of a target. With appropriate optics, these systems are potentially able to image blood cells in vivo as they flow through a vessel, eliminating the need for a blood draw and sample staining. Our group has evaluated the capability of a commercial Snapshot Hyper-Spectral imaging system, the Arrow system from Rebellion Photonics, in differentiating between white and red blood cells on unstained blood smear slides. We evaluated the imaging capabilities of this hyperspectral camera; attached to a microscope at varying objective powers and illumination intensity. Hyperspectral data consisting of 25, 443x313 hyperspectral bands with ~3nm spacing were captured over the range of 419 to 494nm. Open-source hyper-spectral data cube analysis tools, used primarily in Geographic Information Systems (GIS) applications, indicate that white blood cells features are most prominent in the 428-442nm band for blood samples viewed under 20x and 50x magnification over a varying range of illumination intensities. These images could potentially be used in subsequent automated white blood cell segmentation and counting algorithms for performing in vivo white blood cell counting.

  4. Visible hyperspectral imaging for standoff detection of explosives on surfaces

    NASA Astrophysics Data System (ADS)

    Bernacki, Bruce E.; Blake, Thomas A.; Mendoza, Albert; Johnson, Timothy J.

    2010-10-01

    There is an ever-increasing need to be able to detect the presence of explosives, preferably from standoff distances of tens of meters. This paper presents an application of visible hyperspectral imaging using anomaly, polarization, and spectral identification approaches for the standoff detection (13 meters) of nitroaromatic explosives on realistic painted surfaces based upon the colorimetric differences between tetryl and TNT which are enhanced by solar irradiation.

  5. A hyperspectral image data exploration workbench for environmental science applications

    SciTech Connect

    Woyna, M.A.; Christiansen, J.H.; Zawada, D.G.; Simunich, K.L.

    1994-08-01

    The Hyperspectral Image Data Exploration Workbench (HIDEW) software system has been developed by Argonne National Laboratory to enable analysts at Unix workstations to conveniently access and manipulate high-resolution imagery data for analysis, mapping purposes, and input to environmental modeling applications. HIDEW is fully object-oriented, including the underlying database. This system was developed as an aid to site characterization work and atmospheric research projects.

  6. Hyperspectral cathodoluminescence imaging of modern and fossil carbonate shells

    NASA Astrophysics Data System (ADS)

    England, Jennifer; Cusack, Maggie; Paterson, Niall W.; Edwards, Paul; Lee, Martin R.; Martin, Robert

    2006-09-01

    Optical cathodoluminescence (CL) is commonly used to identify diagenetically altered carbonate fossils, yet such an interpretation is problematic as present-day carbonate shells may also luminesce. Hyperspectral CL imaging combines CL microscopy and CL spectroscopy to quantitatively analyze luminescence emission. Cold optical CL and hyperspectral CL imaging were carried out on four modern biominerals, a Rhynchonelliform brachiopod, a Craniid brachiopod, a bivalve, and the eggshell of the domestic fowl. A fossil Craniid brachiopod was analyzed to compare luminescence emission with that from the modern Craniid brachiopod. The beam conditions used for optical CL vary between studies, which hinders the direct comparison of CL analyses. This study assesses the effect of beam current and beam diameter on the intensity of luminescence emission. By characterizing the effect of beam conditions on different CaCO3 biominerals, comparisons can be made between CL studies. Hyperspectral CL imaging can be carried out in combination with WDS element analysis. By comparing hyperspectral CL images with element maps the causes of luminescence can to some extent be determined. The intensity of luminescence emitted from the modern biominerals differs under the same beam conditions. All four modern shells emit blue luminescence. In N. anomala, there is a correlation between Mn2+ concentration and luminescence intensity in the 620- to 630-nm wavelength band, which is apparent in the inner region of the shell. The fossil Craniid also emits blue luminescence, and texture within the shell wall is apparent; however, the luminescence emission between 620 and 630 nm that is evident in N. anomala is absent.

  7. Hyperspectral imaging technique for determination of pork freshness attributes

    NASA Astrophysics Data System (ADS)

    Li, Yongyu; Zhang, Leilei; Peng, Yankun; Tang, Xiuying; Chao, Kuanglin; Dhakal, Sagar

    2011-06-01

    Freshness of pork is an important quality attribute, which can vary greatly in storage and logistics. The specific objectives of this research were to develop a hyperspectral imaging system to predict pork freshness based on quality attributes such as total volatile basic-nitrogen (TVB-N), pH value and color parameters (L*,a*,b*). Pork samples were packed in seal plastic bags and then stored at 4°C. Every 12 hours. Hyperspectral scattering images were collected from the pork surface at the range of 400 nm to 1100 nm. Two different methods were performed to extract scattering feature spectra from the hyperspectral scattering images. First, the spectral scattering profiles at individual wavelengths were fitted accurately by a three-parameter Lorentzian distribution (LD) function; second, reflectance spectra were extracted from the scattering images. Partial Least Square Regression (PLSR) method was used to establish prediction models to predict pork freshness. The results showed that the PLSR models based on reflectance spectra was better than combinations of LD "parameter spectra" in prediction of TVB-N with a correlation coefficient (r) = 0.90, a standard error of prediction (SEP) = 7.80 mg/100g. Moreover, a prediction model for pork freshness was established by using a combination of TVB-N, pH and color parameters. It could give a good prediction results with r = 0.91 for pork freshness. The research demonstrated that hyperspectral scattering technique is a valid tool for real-time and nondestructive detection of pork freshness.

  8. Spatial and spectral performance of a chromotomosynthetic hyperspectral imaging system.

    PubMed

    Bostick, Randall L; Perram, Glen P

    2012-03-01

    The spatial and spectral resolutions achievable by a prototype rotating prism chromotomosynthetic imaging (CTI) system operating in the visible spectrum are described. The instrument creates hyperspectral imagery by collecting a set of 2D images with each spectrally projected at a different rotation angle of the prism. Mathematical reconstruction techniques that have been well tested in the field of medical physics are used to reconstruct the data to produce the 3D hyperspectral image. The instrument operates with a 100 mm focusing lens in the spectral range of 400-900 nm with a field of view of 71.6 mrad and angular resolution of 0.8-1.6 μrad. The spectral resolution is 0.6 nm at the shortest wavelengths, degrading to over 10 nm at the longest wavelengths. Measurements using a point-like target show that performance is limited by chromatic aberration. The system model is slightly inaccurate due to poor estimation of detector spatial resolution, this is corrected based on results improving model performance. As with traditional dispersion technology, calibration of the transformed wavelength axis is required, though with this technology calibration improves both spectral and spatial resolution. While this prototype does not operate at high speeds, components exist which will allow for CTI systems to generate hyperspectral video imagery at rates greater than 100 Hz. PMID:22462909

  9. Spatial and spectral performance of a chromotomosynthetic hyperspectral imaging system

    NASA Astrophysics Data System (ADS)

    Bostick, Randall L.; Perram, Glen P.

    2012-03-01

    The spatial and spectral resolutions achievable by a prototype rotating prism chromotomosynthetic imaging (CTI) system operating in the visible spectrum are described. The instrument creates hyperspectral imagery by collecting a set of 2D images with each spectrally projected at a different rotation angle of the prism. Mathematical reconstruction techniques that have been well tested in the field of medical physics are used to reconstruct the data to produce the 3D hyperspectral image. The instrument operates with a 100 mm focusing lens in the spectral range of 400-900 nm with a field of view of 71.6 mrad and angular resolution of 0.8-1.6 μrad. The spectral resolution is 0.6 nm at the shortest wavelengths, degrading to over 10 nm at the longest wavelengths. Measurements using a point-like target show that performance is limited by chromatic aberration. The system model is slightly inaccurate due to poor estimation of detector spatial resolution, this is corrected based on results improving model performance. As with traditional dispersion technology, calibration of the transformed wavelength axis is required, though with this technology calibration improves both spectral and spatial resolution. While this prototype does not operate at high speeds, components exist which will allow for CTI systems to generate hyperspectral video imagery at rates greater than 100 Hz.

  10. Hyperspectral image analysis for standoff trace detection using IR laser spectroscopy

    NASA Astrophysics Data System (ADS)

    Jarvis, J.; Fuchs, F.; Hugger, S.; Ostendorf, R.; Butschek, L.; Yang, Q.; Dreyhaupt, A.; Grahmann, J.; Wagner, J.

    2016-05-01

    In the recent past infrared laser backscattering spectroscopy using Quantum Cascade Lasers (QCL) emitting in the molecular fingerprint region between 7.5 μm and 10 μm proved a highly promising approach for stand-off detection of dangerous substances. In this work we present an active illumination hyperspectral image sensor, utilizing QCLs as spectral selective illumination sources. A high performance Mercury Cadmium Telluride (MCT) imager is used for collection of the diffusely backscattered light. Well known target detection algorithms like the Adaptive Matched Subspace Detector and the Adaptive Coherent Estimator are used to detect pixel vectors in the recorded hyperspectral image that contain traces of explosive substances like PETN, RDX or TNT. In addition we present an extension of the backscattering spectroscopy technique towards real-time detection using a MOEMS EC-QCL.

  11. Infrared hyperspectral imaging results from vapor plume experiments

    SciTech Connect

    Bennett, C.L.; Carter, M.R.; Fields, D.J.

    1995-04-17

    In this article, recent measurements made with LIFTIRS, the Livermore Imaging Fourier Transform InfraRed Spectrometer, are presented. The experience gained with this instrument has produced a variety of insights into the tradeoffs between signal to noise ratio (SNR), spectral resolution and temporal resolution for time multiplexed Fourier transform imaging spectrometers. This experience has also clarified the practical advantages and disadvantages of Fourier transform hyperspectral imaging spectrometers regarding adaptation to varying measurement requirements on SNR vs. spectral resolution, spatial resolution and temporal resolution.

  12. A short wave infrared hyperspectral imager for landmine detection

    NASA Astrophysics Data System (ADS)

    McFee, John E.; Achal, Steve; Ivanco, Tyler; Anger, Cliff

    2005-06-01

    DRDC Suffield and Itres Research have jointly investigated the use of visible and infrared hyperspectral imaging for landmine detection since 1988. There has been considerable success detecting surface-laid landmines by classification of their visible/near infrared (VNIR - 400 to 1000 nm wavelength) spectral signatures, but it has not been possible to find VNIR spectral characteristics that would generically distinguish anthropogenic objects from natural features such as rocks, vegetation, soil, etc. Preliminary studies in 1998 suggested that it might be possible to develop such a generic classifier in the short wave infrared (SWIR) and that detection performance might improve. Because of a lack of available SWIR hyperspectral imagers with adequate performance for mine detection, a prototype pushbroom SWIR hyperspectral imager was developed and completed in summer 2002. The now commercially available instrument, sasi, has 160 bands over a spectral range of 850 to 2450 nm, signal to noise ratio of 400:1 with f/1.8 fore-optics, and 600 pixels over a 37.7° field of view. A number of mission flights have been carried out and excellent imagery obtained. In October 2003, Itres and DRDC Suffield personnel obtained field SWIR hyperspectral imagery in the DRDC Suffield Mine Pen of numerous surface-laid mines, one buried mine, other surface-laid human-made items, background materials and people from a horizontally scanning personnel-lift at an altitude of roughly 5 m. Preliminary indications are that a simple generic classification decision boundary should be able to distinguish surface-laid landmines from many human-made artifacts and natural materials. The buried mine was not detected, but the mine had been buried for several years and hence there would be no residual surface disturbance. Furthermore, the small sample size and limited observation time make it difficult to generalize about SWIR performance for buried mines. The instrument is described and the preliminary

  13. Hyperspectral image segmentation using a cooperative nonparametric approach

    NASA Astrophysics Data System (ADS)

    Taher, Akar; Chehdi, Kacem; Cariou, Claude

    2013-10-01

    In this paper a new unsupervised nonparametric cooperative and adaptive hyperspectral image segmentation approach is presented. The hyperspectral images are partitioned band by band in parallel and intermediate classification results are evaluated and fused, to get the final segmentation result. Two unsupervised nonparametric segmentation methods are used in parallel cooperation, namely the Fuzzy C-means (FCM) method, and the Linde-Buzo-Gray (LBG) algorithm, to segment each band of the image. The originality of the approach relies firstly on its local adaptation to the type of regions in an image (textured, non-textured), and secondly on the introduction of several levels of evaluation and validation of intermediate segmentation results before obtaining the final partitioning of the image. For the management of similar or conflicting results issued from the two classification methods, we gradually introduced various assessment steps that exploit the information of each spectral band and its adjacent bands, and finally the information of all the spectral bands. In our approach, the detected textured and non-textured regions are treated separately from feature extraction step, up to the final classification results. This approach was first evaluated on a large number of monocomponent images constructed from the Brodatz album. Then it was evaluated on two real applications using a respectively multispectral image for Cedar trees detection in the region of Baabdat (Lebanon) and a hyperspectral image for identification of invasive and non invasive vegetation in the region of Cieza (Spain). A correct classification rate (CCR) for the first application is over 97% and for the second application the average correct classification rate (ACCR) is over 99%.

  14. Identification of staphylococcus species with hyperspectral microscope imaging and classification algrorithms

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral microscope imaging is presented as a rapid and efficient tool to classify foodborne bacteria species. The spectral data were obtained from five different species of Staphylococcus spp. with a hyperspectral microscope imaging system that provided a maximum of 89 contiguous spectral imag...

  15. Visible to SWIR hyperspectral imaging for produce safety and quality evaluation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral imaging techniques, combining the advantages of spectroscopy and imaging, have found wider use in food quality and safety evaluation applications during the past decade. In light of the prevalent use of hyperspectral imaging techniques in the visible to near-infrared (VNIR: 400 -1000 n...

  16. PARTIAL LEAST SQUARES REGRESSION OF HYPERSPECTRAL IMAGES FOR CONTAMINATION DETECTION ON POULTRY CARCASSES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Abstract The U.S. Department of Agriculture has developed multispectral and hyperspectral imaging systems to detect faecal contaminants. Until recently, the hyperspectral imaging system has been used as a research tool to detect a few optimum wavelengths for use in a multispectral imaging system. ...

  17. Partial Least Squares Regression of Hyperspectral Images for Contaminant Detection on Poultry Carcasses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The U.S. Department of Agriculture has developed multispectral and hyperspectral imaging systems to detect faecal contaminants. Until recently, the hyperspectral imaging system has been used as a research tool to detect a few optimum wavelengths for use in a multispectral imaging system. However, ...

  18. Detection of early plant stress responses in hyperspectral images

    NASA Astrophysics Data System (ADS)

    Behmann, Jan; Steinrücken, Jörg; Plümer, Lutz

    2014-07-01

    Early stress detection in crop plants is highly relevant, but hard to achieve. We hypothesize that close range hyperspectral imaging is able to uncover stress related processes non-destructively in the early stages which are invisible to the human eye. We propose an approach which combines unsupervised and supervised methods in order to identify several stages of progressive stress development from series of hyperspectral images. Stress of an entire plant is detected by stress response levels at pixel scale. The focus is on drought stress in barley (Hordeum vulgare). Unsupervised learning is used to separate hyperspectral signatures into clusters related to different stages of stress response and progressive senescence. Whereas all such signatures may be found in both, well watered and drought stressed plants, their respective distributions differ. Ordinal classification with Support Vector Machines (SVM) is used to quantify and visualize the distribution of progressive stages of senescence and to separate well watered from drought stressed plants. For each senescence stage a distinctive set of most relevant Vegetation Indices (VIs) is identified. The method has been applied on two experiments involving potted barley plants under well watered and drought stress conditions in a greenhouse. Drought stress is detected up to ten days earlier than using NDVI. Furthermore, it is shown that some VIs have overall relevance, while others are specific to particular senescence stages. The transferability of the method to the field is illustrated by an experiment on maize (Zea mays).

  19. Reconstruction of hyperspectral image using matting model for classification

    NASA Astrophysics Data System (ADS)

    Xie, Weiying; Li, Yunsong; Ge, Chiru

    2016-05-01

    Although hyperspectral images (HSIs) captured by satellites provide much information in spectral regions, some bands are redundant or have large amounts of noise, which are not suitable for image analysis. To address this problem, we introduce a method for reconstructing the HSI with noise reduction and contrast enhancement using a matting model for the first time. The matting model refers to each spectral band of an HSI that can be decomposed into three components, i.e., alpha channel, spectral foreground, and spectral background. First, one spectral band of an HSI with more refined information than most other bands is selected, and is referred to as an alpha channel of the HSI to estimate the hyperspectral foreground and hyperspectral background. Finally, a combination operation is applied to reconstruct the HSI. In addition, the support vector machine (SVM) classifier and three sparsity-based classifiers, i.e., orthogonal matching pursuit (OMP), simultaneous OMP, and OMP based on first-order neighborhood system weighted classifiers, are utilized on the reconstructed HSI and the original HSI to verify the effectiveness of the proposed method. Specifically, using the reconstructed HSI, the average accuracy of the SVM classifier can be improved by as much as 19%.

  20. Hyperspectral image analysis for CARS, SRS, and Raman data

    PubMed Central

    Karuna, Arnica; Borri, Paola; Langbein, Wolfgang

    2015-01-01

    In this work, we have significantly enhanced the capabilities of the hyperspectral image analysis (HIA) first developed by Masia et al. 1 The HIA introduced a method to factorize the hyperspectral data into the product of component concentrations and spectra for quantitative analysis of the chemical composition of the sample. The enhancements shown here comprise (1) a spatial weighting to reduce the spatial variation of the spectral error, which improves the retrieval of the chemical components with significant local but small global concentrations; (2) a new selection criterion for the spectra used when applying sparse sampling2 to speed up sequential hyperspectral imaging; and (3) a filter for outliers in the data using singular value decomposition, suited e.g. to suppress motion artifacts. We demonstrate the enhancements on coherent anti‐Stokes Raman scattering, stimulated Raman scattering, and spontaneous Raman data. We provide the HIA software as executable for public use. © 2015 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons, Ltd. PMID:27478301

  1. Semi-supervised feature learning for hyperspectral image classification

    NASA Astrophysics Data System (ADS)

    Zhang, Pengfei; Cao, Liujuan; Wang, Cheng; Li, Jonathan

    2016-03-01

    Hyperspectral image has high-dimensional Spectral-spatial features, those features with some noisy and redundant information. Since redundant features can have significant adverse effect on learning performance. So efficient and robust feature selection methods are make the best of labeled and unlabeled points to extract meaningful features and eliminate noisy ones. On the other hand, obtaining sufficient accurate labeled data is either impossible or expensive. In order to take advantage of both precious labeled and unlabeled data points, in this paper, we propose a new semisupervised feature selection method, Firstly, we use labeled points are to enlarge the margin between data points from different classes; Secondly, we use unlabeled points to find the local structure of the data space; Finally, we compare our proposed algorithm with Fisher score, PCA and Laplacian score on HSI classification. Experimental results on benchmark hyperspectral data sets demonstrate the efficiency and effectiveness of our proposed algorithm.

  2. AOTF hyperspectral microscopic imaging for foodborne pathogenic bacteria detection

    NASA Astrophysics Data System (ADS)

    Park, Bosoon; Lee, Sangdae; Yoon, Seung-Chul; Sundaram, Jaya; Windham, William R.; Hinton, Arthur, Jr.; Lawrence, Kurt C.

    2011-06-01

    Hyperspectral microscope imaging (HMI) method which provides both spatial and spectral information can be effective for foodborne pathogen detection. The AOTF-based hyperspectral microscope imaging method can be used to characterize spectral properties of biofilm formed by Salmonella enteritidis as well as Escherichia coli. The intensity of spectral imagery and the pattern of spectral distribution varied with system parameters (integration time and gain) of HMI system. The preliminary results demonstrated determination of optimum parameter values of HMI system and the integration time must be no more than 250 ms for quality image acquisition from biofilm formed by S. enteritidis. Among the contiguous spectral imagery between 450 and 800 nm, the intensity of spectral images at 498, 522, 550 and 594 nm were distinctive for biofilm; whereas, the intensity of spectral images at 546 nm was distinctive for E. coli. For more accurate comparison of intensity from spectral images, a calibration protocol, using neutral density filters and multiple exposures, need to be developed to standardize image acquisition. For the identification or classification of unknown food pathogen samples, ground truth regions-of-interest pixels need to be selected for "spectrally pure fingerprints" for the Salmonella and E. coli species.

  3. Characterizing pigments with hyperspectral imaging variable false-color composites

    NASA Astrophysics Data System (ADS)

    Hayem-Ghez, Anita; Ravaud, Elisabeth; Boust, Clotilde; Bastian, Gilles; Menu, Michel; Brodie-Linder, Nancy

    2015-11-01

    Hyperspectral imaging has been used for pigment characterization on paintings for the last 10 years. It is a noninvasive technique, which mixes the power of spectrophotometry and that of imaging technologies. We have access to a visible and near-infrared hyperspectral camera, ranging from 400 to 1000 nm in 80-160 spectral bands. In order to treat the large amount of data that this imaging technique generates, one can use statistical tools such as principal component analysis (PCA). To conduct the characterization of pigments, researchers mostly use PCA, convex geometry algorithms and the comparison of resulting clusters to database spectra with a specific tolerance (like the Spectral Angle Mapper tool on the dedicated software ENVI). Our approach originates from false-color photography and aims at providing a simple tool to identify pigments thanks to imaging spectroscopy. It can be considered as a quick first analysis to see the principal pigments of a painting, before using a more complete multivariate statistical tool. We study pigment spectra, for each kind of hue (blue, green, red and yellow) to identify the wavelength maximizing spectral differences. The case of red pigments is most interesting because our methodology can discriminate the red pigments very well—even red lakes, which are always difficult to identify. As for the yellow and blue categories, it represents a good progress of IRFC photography for pigment discrimination. We apply our methodology to study the pigments on a painting by Eustache Le Sueur, a French painter of the seventeenth century. We compare the results to other noninvasive analysis like X-ray fluorescence and optical microscopy. Finally, we draw conclusions about the advantages and limits of the variable false-color image method using hyperspectral imaging.

  4. Spatial regularization for the unmixing of hyperspectral images

    NASA Astrophysics Data System (ADS)

    Bauer, Sebastian; Neumann, Florian; Puente León, Fernando

    2015-05-01

    For demanding sorting tasks, the acquisition and processing of color images does not provide sufficient information for the successful discrimination between the different object classes that are to be sorted. An alternative to integrating three spectral regions of visible light to the three color channels is to sample the spectrum at up to several hundred, evenly-spaced points and acquire so-called hyperspectral images. Such images provide a complete image of the scene at each considered wavelength and contain much more information about the composition of the different materials. Hyperspectral images can also be acquired in spectral regions neighboring visible light such as, e.g., the ultraviolet (UV) and near-infrared (NIR) region. From a mathematical point of view, it is possible to extract the spectra of the pure materials and the amount to which these spectra contribute to material mixtures. This process is called spectral unmixing. Spectral unmixing based on the mostly used linear mixing model is a difficult task due to model ambiguities and distorting factors such as noise. Until a few years ago, the most inherent property of hyperspectral images, that is to say, the abundance correlation between neighboring pixels, was not used in unmixing algorithms. Only recently, researchers started to incorporate spatial information into the unmixing process, which by now is known to improve the unmixing results. In this paper, we will introduce two new methods and study the effect of these two and two already described methods on spectral unmixing, especially on their ability to account for edges and other shapes in the abundance maps.

  5. Measured performance of an airborne Fourier-transform hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Otten, Leonard John, III; Meigs, Andrew D.; Sellar, R. Glenn; Rafert, Bruce

    1996-11-01

    A new hyperspectral imager has recently been developed by Kestrel Corporation for use in light aircraft platforms. The instrument provides 256 spectral channels with 87 cm-1 spectral bandwidth over the 450 nm to 1000 nm portion of the spectrum. Operated as a pushbroom imager, the FTVHSI has been shown to have a IFOV of 0.75 mrad, and a FOV of 0.23 rad. The sensor includes an internal spectral/radiometric calibration source, a self contained spectrally resolved downwelling sensor, and complete line of sight and GPS positioning information. The instrument is now operating from a Cessna TU-206 single engine aircraft.

  6. Transillumination hyperspectral imaging for histopathological examination of excised tissue

    NASA Astrophysics Data System (ADS)

    Vasefi, Fartash; Najiminaini, Mohamadreza; Ng, Eldon; Chamson-Reig, Astrid; Kaminska, Bozena; Brackstone, Muriel; Carson, Jeffrey

    2011-08-01

    Angular domain spectroscopic imaging (ADSI) is a novel technique for the detection and characterization of optical contrast in turbid media based on spectral characteristics. The imaging system employs a silicon micromachined angular filter array to reject scattered light traversing a specimen and an imaging spectrometer to capture and discriminate the largely remaining quasiballistic light based on spatial position and wavelength. The imaging modality results in hyperspectral shadowgrams containing two-dimensional (2D) spatial maps of spectral information. An ADSI system was constructed and its performance was evaluated in the near-infrared region on tissue-mimicking phantoms. Image-based spectral correlation analysis using transmission spectra and first order derivatives revealed that embedded optical targets could be resolved. The hyperspectral images obtained with ADSI were observed to depend on target concentration, target depth, and scattering level of the background medium. A similar analysis on a muscle and tumor sample dissected from a mouse resulted in spatially dependent optical transmission spectra that were distinct, which suggested that ADSI may find utility in classifying tissues in biomedical applications.

  7. Evaluation of a hyperspectral image database for demosaicking purposes

    NASA Astrophysics Data System (ADS)

    Larabi, Mohamed-Chaker; Süsstrunk, Sabine

    2011-01-01

    We present a study on the the applicability of hyperspectral images to evaluate color filter array (CFA) design and the performance of demosaicking algorithms. The aim is to simulate a typical digital still camera processing pipe-line and to compare two different scenarios: evaluate the performance of demosaicking algorithms applied to raw camera RGB values before color rendering to sRGB, and evaluate the performance of demosaicking algorithms applied on the final sRGB color rendered image. The second scenario is the most frequently used one in literature because CFA design and algorithms are usually tested on a set of existing images that are already rendered, such as the Kodak Photo CD set containing the well-known lighthouse image. We simulate the camera processing pipe-line with measured spectral sensitivity functions of a real camera. Modeling a Bayer CFA, we select three linear demosaicking techniques in order to perform the tests. The evaluation is done using CMSE, CPSNR, s-CIELAB and MSSIM metrics to compare demosaicking results. We find that the performance, and especially the difference between demosaicking algorithms, is indeed significant depending if the mosaicking/demosaicking is applied to camera raw values as opposed to already rendered sRGB images. We argue that evaluating the former gives a better indication how a CFA/demosaicking combination will work in practice, and that it is in the interest of the community to create a hyperspectral image dataset dedicated to that effect.

  8. Hyperspectral image segmentation using spatial-spectral graphs

    NASA Astrophysics Data System (ADS)

    Gillis, David B.; Bowles, Jeffrey H.

    2012-06-01

    Spectral graph theory has proven to be a useful tool in the analysis of high-dimensional data sets. Recall that, mathematically, a graph is a collection of objects (nodes) and connections between them (edges); a weighted graph additionally assigns numerical values (weights) to the edges. Graphs are represented by their adjacency whose elements are the weights between the nodes. Spectral graph theory uses the eigendecomposition of the adjacency matrix (or, more generally, the Laplacian of the graph) to derive information about the underlying graph. In this paper, we develop a spectral method based on the 'normalized cuts' algorithm to segment hyperspectral image data (HSI). In particular, we model an image as a weighted graph whose nodes are the image pixels, and edges defined as connecting spatial neighbors; the edge weights are given by a weighted combination of the spatial and spectral distances between nodes. We then use the Laplacian of the graph to recursively segment the image. The advantages of our approach are that, first, the graph structure naturally incorporates both the spatial and spectral information present in HSI; also, by using only spatial neighbors, the adjacency matrix is highly sparse; as a result, it is possible to apply our technique to much larger images than previous techniques. In the paper, we present the details of our algorithm, and include experimental results from a variety of hyperspectral images.

  9. Superpixel-Augmented Endmember Detection for Hyperspectral Images

    NASA Technical Reports Server (NTRS)

    Thompson, David R.; Castano, Rebecca; Gilmore, Martha

    2011-01-01

    Superpixels are homogeneous image regions comprised of several contiguous pixels. They are produced by shattering the image into contiguous, homogeneous regions that each cover between 20 and 100 image pixels. The segmentation aims for a many-to-one mapping from superpixels to image features; each image feature could contain several superpixels, but each superpixel occupies no more than one image feature. This conservative segmentation is relatively easy to automate in a robust fashion. Superpixel processing is related to the more general idea of improving hyperspectral analysis through spatial constraints, which can recognize subtle features at or below the level of noise by exploiting the fact that their spectral signatures are found in neighboring pixels. Recent work has explored spatial constraints for endmember extraction, showing significant advantages over techniques that ignore pixels relative positions. Methods such as AMEE (automated morphological endmember extraction) express spatial influence using fixed isometric relationships a local square window or Euclidean distance in pixel coordinates. In other words, two pixels covariances are based on their spatial proximity, but are independent of their absolute location in the scene. These isometric spatial constraints are most appropriate when spectral variation is smooth and constant over the image. Superpixels are simple to implement, efficient to compute, and are empirically effective. They can be used as a preprocessing step with any desired endmember extraction technique. Superpixels also have a solid theoretical basis in the hyperspectral linear mixing model, making them a principled approach for improving endmember extraction. Unlike existing approaches, superpixels can accommodate non-isometric covariance between image pixels (characteristic of discrete image features separated by step discontinuities). These kinds of image features are common in natural scenes. Analysts can substitute superpixels

  10. Research on method of geometry and spectral calibration of pushbroom dispersive hyperspectral imager

    NASA Astrophysics Data System (ADS)

    He, Zhiping; Shu, Rong; Wang, Jianyu

    2012-11-01

    Development and application of airborne and aerospace hyperspectral imager press for high precision geometry and spectral calibration of pixels of image cube. The research of geometry and spectral calibration of pushbroom hyperspectral imager, its target is giving the coordinate of angle field of view and center wavelength of each detect unit in focal plane detector of hyperspectral imager, and achieves the high precision, full field of view, full channel geometry and spectral calibration. It is importance for imaging quantitative and deep application of hyperspectal imager. The paper takes the geometry and spectral calibration of pushbroom dispersive hyperspectral imager as case study, and research on the constitution and analysis of imaging mathematical model. Aimed especially at grating-dispersive hyperspectral imaging, the specialty of the imaging mode and dispersive method has been concretely analyzed. Based on the analysis, the theory and feasible method of geometry and spectral calibration of dispersive hyperspectral imager is set up. The key technique has been solved is As follows: 1). the imaging mathematical model and feasible method of geometry and spectral calibration for full pixels of image cube has been set up, the feasibility of the calibration method has been analyzed. 2). the engineering model and method of the geometry and spectral calibration of pushbroom dispersive hyperspectral imager has been set up and the calibration equipment has been constructed, and the calibration precision has been analyzed.

  11. Next generation miniature simultaneous multi-hyperspectral imaging systems

    NASA Astrophysics Data System (ADS)

    Hinnrichs, Michele; Gupta, Neelam

    2014-03-01

    The concept for a hyperspectral imaging system using a Fabry-Perot tunable filter (FPTF) array that is fabricated using "miniature optical electrical mechanical system" (MOEMS) technology. [1] Using an array of FPTF as an approach to hyperspectral imaging relaxes wavelength tuning requirements considerably because of the reduced portion of the spectrum that is covered by each element in the array. In this paper, Pacific Advanced Technology and ARL present the results of a concept design and performed analysis of a MOEMS based tunable Fabry-Perot array (FPTF) to perform simultaneous multispectral and hyperspectral imaging with relatively high spatial resolution. The concept design was developed with support of an Army SBIR Phase I program The Fabry-Perot tunable MOEMS filter array was combined with a miniature optics array and a focal plane array of 1024 x 1024 pixels to produce 16 colors every frame of the camera. Each color image has a spatial resolution of 256 x 256 pixels with an IFOV of 1.7 mrads and FOV of 25 degrees. The spectral images are collected simultaneously allowing high resolution spectral-spatial-temporal information in each frame of the camera, thus enabling the implementation of spectral-temporal-spatial algorithms in real-time to provide high sensitivity for the detection of weak signals in a high clutter background environment with low sensitivity to camera motion. The challenge in the design was the independent actuation of each Fabry Perot element in the array allowing for individual tuning. An additional challenge was the need to maximize the fill factor to improve the spatial coverage with minimal dead space. This paper will only address the concept design and analysis of the Fabry-Perot tunable filter array. A previous paper presented at SPIE DSS in 2012 explained the design of the optical array.

  12. Single aflatoxin contaminated corn kernel analysis with fluorescence hyperspectral image

    NASA Astrophysics Data System (ADS)

    Yao, Haibo; Hruska, Zuzana; Kincaid, Russell; Ononye, Ambrose; Brown, Robert L.; Cleveland, Thomas E.

    2010-04-01

    Aflatoxins are toxic secondary metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus, among others. Aflatoxin contaminated corn is toxic to domestic animals when ingested in feed and is a known carcinogen associated with liver and lung cancer in humans. Consequently, aflatoxin levels in food and feed are regulated by the Food and Drug Administration (FDA) in the US, allowing 20 ppb (parts per billion) limits in food and 100 ppb in feed for interstate commerce. Currently, aflatoxin detection and quantification methods are based on analytical tests including thin-layer chromatography (TCL) and high performance liquid chromatography (HPLC). These analytical tests require the destruction of samples, and are costly and time consuming. Thus, the ability to detect aflatoxin in a rapid, nondestructive way is crucial to the grain industry, particularly to corn industry. Hyperspectral imaging technology offers a non-invasive approach toward screening for food safety inspection and quality control based on its spectral signature. The focus of this paper is to classify aflatoxin contaminated single corn kernels using fluorescence hyperspectral imagery. Field inoculated corn kernels were used in the study. Contaminated and control kernels under long wavelength ultraviolet excitation were imaged using a visible near-infrared (VNIR) hyperspectral camera. The imaged kernels were chemically analyzed to provide reference information for image analysis. This paper describes a procedure to process corn kernels located in different images for statistical training and classification. Two classification algorithms, Maximum Likelihood and Binary Encoding, were used to classify each corn kernel into "control" or "contaminated" through pixel classification. The Binary Encoding approach had a slightly better performance with accuracy equals to 87% or 88% when 20 ppb or 100 ppb was used as classification threshold, respectively.

  13. Hyperspectral imaging system for disease scanning on banana plants

    NASA Astrophysics Data System (ADS)

    Ochoa, Daniel; Cevallos, Juan; Vargas, German; Criollo, Ronald; Romero, Dennis; Castro, Rodrigo; Bayona, Oswaldo

    2016-05-01

    Black Sigatoka (BS) is a banana plant disease caused by the fungus Mycosphaerella fijiensis. BS symptoms can be observed at late infection stages. By that time, BS has probably spread to other plants. In this paper, we present our current work on building an hyper-spectral (HS) imaging system aimed at in-vivo detection of BS pre-symptomatic responses in banana leaves. The proposed imaging system comprises a motorized stage, a high-sensitivity VIS-NIR camera and an optical spectrograph. To capture images of the banana leaf, the stage's speed and camera's frame rate must be computed to reduce motion blur and to obtain the same resolution along both spatial dimensions of the resulting HS cube. Our continuous leaf scanning approach allows imaging leaves of arbitrary length with minimum frame loss. Once the images are captured, a denoising step is performed to improve HS image quality and spectral profile extraction.

  14. Hyperspectral imaging in the infrared using LIFTIRS

    SciTech Connect

    Bennett, C.L.; Carter, M.R.; Fields, D.J.

    1995-07-01

    In this article, recent characterization measurements made with LIFTIRS, the Livermore Imaging Fourier Transform InfraRed Spectrometer, are presented. A discussion is also presented of the relative merits of the various alternative designs for imaging spectrometers.

  15. Multichannel tunable imager architecture for hyperspectral imaging in relevant spectral domains.

    PubMed

    Goenka, Chhavi; Semeter, Joshua; Noto, John; Baumgardner, Jeffrey; Riccobono, Juanita; Migliozzi, Mike; Dahlgren, Hanna; Marshall, Robert; Kapali, Sudha; Hirsch, Michael; Hampton, Donald; Akbari, Hassanali

    2016-04-20

    In this paper, we present a technique for dimensionality reduction in hyperspectral imaging during the data collection process. A four-channel hyperspectral imager using liquid crystal Fabry-Perot etalons has been built and used to verify this method for four applications: auroral imaging, plant study, landscape classification, and anomaly detection. This imager is capable of making measurements simultaneously in four wavelength ranges while being tunable within those ranges, and thus can be used to measure narrow contiguous bands in four spectral domains. In this paper, we describe the design, concept of operation, and deployment of this instrument. The results from preliminary testing of this instrument are discussed and are promising and demonstrate this instrument as a good candidate for hyperspectral imaging. PMID:27140081

  16. Image enhancement based on in vivo hyperspectral gastroscopic images: a case study.

    PubMed

    Gu, Xiaozhou; Han, Zhimin; Yao, Liqing; Zhong, Yunshi; Shi, Qiang; Fu, Ye; Liu, Changsheng; Wang, Xiguang; Xie, Tianyu

    2016-10-01

    Hyperspectral imaging (HSI) has been recognized as a powerful tool for noninvasive disease detection in the gastrointestinal field. However, most of the studies on HSI in this field have involved ex vivo biopsies or resected tissues. We proposed an image enhancement method based on in vivo hyperspectral gastroscopic images. First, we developed a flexible gastroscopy system capable of obtaining in vivo hyperspectral images of different types of stomach disease mucosa. Then, depending on a specific object, an appropriate band selection algorithm based on dependence of information was employed to determine a subset of spectral bands that would yield useful spatial information. Finally, these bands were assigned to be the color components of an enhanced image of the object. A gastric ulcer case study demonstrated that our method yields higher color tone contrast, which enhanced the displays of the gastric ulcer regions, and that it will be valuable in clinical applications. PMID:27206742

  17. Quantitative wavelength analysis and image classification for intraoperative cancer diagnosis with hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Lu, Guolan; Qin, Xulei; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

    2015-03-01

    Complete surgical removal of tumor tissue is essential for postoperative prognosis after surgery. Intraoperative tumor imaging and visualization are an important step in aiding surgeons to evaluate and resect tumor tissue in real time, thus enabling more complete resection of diseased tissue and better conservation of healthy tissue. As an emerging modality, hyperspectral imaging (HSI) holds great potential for comprehensive and objective intraoperative cancer assessment. In this paper, we explored the possibility of intraoperative tumor detection and visualization during surgery using HSI in the wavelength range of 450 nm - 900 nm in an animal experiment. We proposed a new algorithm for glare removal and cancer detection on surgical hyperspectral images, and detected the tumor margins in five mice with an average sensitivity and specificity of 94.4% and 98.3%, respectively. The hyperspectral imaging and quantification method have the potential to provide an innovative tool for image-guided surgery.

  18. Quantitative Wavelength Analysis and Image Classification for Intraoperative Cancer Diagnosis with Hyperspectral Imaging

    PubMed Central

    Lu, Guolan; Qin, Xulei; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

    2015-01-01

    Complete surgical removal of tumor tissue is essential for postoperative prognosis after surgery. Intraoperative tumor imaging and visualization are an important step in aiding surgeons to evaluate and resect tumor tissue in real time, thus enabling more complete resection of diseased tissue and better conservation of healthy tissue. As an emerging modality, hyperspectral imaging (HSI) holds great potential for comprehensive and objective intraoperative cancer assessment. In this paper, we explored the possibility of intraoperative tumor detection and visualization during surgery using HSI in the wavelength range of 450 nm - 900 nm in an animal experiment. We proposed a new algorithm for glare removal and cancer detection on surgical hyperspectral images, and detected the tumor margins in five mice with an average sensitivity and specificity of 94.4% and 98.3%, respectively. The hyperspectral imaging and quantification method have the potential to provide an innovative tool for image-guided surgery. PMID:26523083

  19. Embedded Bone Fragment Detection in Chicken Fillets using Transmittance Image Enhancement and Hyperspectral Reflectance Imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper is concerned with the detection of bone fragments embedded in compressed de-boned skinless chicken breast fillets by enhancing single-band transmittance images generated by back-lighting and exploiting spectral information from hyperspectral reflectance images. Optical imaging of chicken ...

  20. POULTRY SKIN TUMOR DETECTION IN HYPERSPECTRAL REFLECTANCE IMAGES BY COMBINING CLASSIFIERS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper presents a new method for detecting poultry skin tumors in hyperspectral reflectance images. We employ the principal component analysis (PCA), discrete wavelet transform (DWT), and kernel discriminant analysis (KDA) to extract the independent feature sets in hyperspectral reflectance imag...

  1. Application of hyperspectral imaging for characterization of intramuscular fat distribution in beef

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this study, a hyperspectral imaging system in the spectral region of 400–1000 nm was used for visualization and determination of intramuscular fat concentration in beef samples. Hyperspectral images were acquired for beef samples, and spectral information was then extracted from each single sampl...

  2. Hyperspectral imaging-based classification and wavebands selection for internal defect detection of pickling cucumbers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral imaging is useful for detecting internal defect of pickling cucumbers. The technique, however, is not yet suitable for high-speed online implementation due to the challenges for analyzing large-scale hyperspectral images. This research was aimed to select the optimal wavebands from the...

  3. Penetration depth measurement of near-infrared hyperspectral imaging light for milk powder

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The increasingly common application of near-infrared (NIR) hyperspectral imaging technique to the analysis of food powders has led to the need for optical characterization of samples. This study was aimed at exploring the feasibility of quantifying penetration depth of NIR hyperspectral imaging ligh...

  4. Measurement of the Absorption and Scattering Properties of Turbid Liquid Foods Using Hyperspectral Imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper reports on the development of a hyperspectral imaging technique for rapid determination of the absorption and scattering properties of turbid liquid foods over the visible and near-infrared region of 530-900 nm. A hyperspectral imaging system in line scanning mode was first tested and val...

  5. On-Line Hyperspectral Transmittance Imaging for Internal Defect Detection of Pickling Cucumbers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hyperspectral imaging technique under transmittance mode was investigated for detection of internal defect in pickling cucumbers such as carpel suture separation or hollow cucumbers caused by mechanical stress. A prototype of on-line hyperspectral transmittance imaging system was developed for real...

  6. First results of ground-based LWIR hyperspectral imaging remote gas detection

    NASA Astrophysics Data System (ADS)

    Zheng, Wei-jian; Lei, Zheng-gang; Yu, Chun-chao; Wang, Hai-yang; Fu, Yan-peng; Liao, Ning-fang; Su, Jun-hong

    2014-11-01

    The new progress of ground-based long-wave infrared remote sensing is presented. The LWIR hyperspectral imaging by using the windowing spatial and temporal modulation Fourier spectroscopy, and the results of outdoor ether gas detection, verify the features of LWIR hyperspectral imaging remote sensing and technical approach. It provides a new technical means for ground-based gas remote sensing.

  7. Hyperspectral microscope imaging methods to classify gram-positive and gram-negative foodborne pathogenic bacteria

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An acousto-optic tunable filter-based hyperspectral microscope imaging method has potential for identification of foodborne pathogenic bacteria from microcolony rapidly with a single cell level. We have successfully developed the method to acquire quality hyperspectral microscopic images from variou...

  8. HICO and RAIDS Experiment Payload - Hyperspectral Imager for the Coastal Ocean

    NASA Technical Reports Server (NTRS)

    Corson, Mike

    2009-01-01

    HICO and RAIDS Experiment Payload - Hyperspectral Imager For The Coastal Ocean (HREP-HICO) will operate a visible and near-infrared (VNIR) Maritime Hyperspectral Imaging (MHSI) system, to detect, identify and quantify coastal geophysical features from the International Space Station.

  9. Differentiation of deciduous-calyx Korla fragrant pears using NIR hyperspectral imaging analysis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Near-infrared hyperspectral imaging was investigated as a potential method for automatic sorting of pears according to their calyx type. The hyperspectral images were analyzed and wavebands at 1190 nm and 1199 nm were selected for differentiating deciduous-calyx fruits from persistent-calyx ones. A ...

  10. Determination of germination quality of cucumber (Cucumis sativus) seed by LED-induced hyperspectral reflectance imaging

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Purpose: We developed a viability evaluation method for cucumber (Cucumis sativus) seed using hyperspectral reflectance imaging. Methods: Reflectance spectra of cucumber seeds in the 400 to 1000 nm range were collected from hyperspectral reflectance images obtained using blue, green, and red LED ill...

  11. Multimodal confocal hyperspectral imaging microscopy with wavelength sweeping source

    NASA Astrophysics Data System (ADS)

    Kim, Young-Duk; Do, Dukho; Yoo, Hongki; Gweon, DaeGab

    2015-02-01

    There exist microscopes that are able to obtain the chemical properties of a sample, because there are some cases in which it is difficult to find out causality of a phenomenon by using only the structural information of a sample. Obtaining the chemical properties of a sample is important in biomedical imaging, because most biological phenomena include changes in the chemical properties of the sample. Hyperspectral imaging (HSI) is one of the popular imaging methods for characterizing materials and biological samples by measuring the reflectance or emission spectrum of the sample. Because all materials have a unique reflectance spectrum, it is possible to analyze material properties and detect changes in the chemical properties of a sample by measuring the spectral changes with respect to the original spectrum. Because of its ability to measure the spectrum of a sample, HSI is widely used in materials identification applications such as aerial reconnaissance and is the subject of various studies in microscopy. Although there are many advantages to using the method, conventional HSI has some limitations because of its complex configuration and slow speed. In this research we propose a new type of multimodal confocal hyperspectral imaging microscopy with fast image acquisition and a simple configuration that is capable of both confocal and HSI microscopies.

  12. Hyperspectral imaging for detection of arthritis: feasibility and prospects

    NASA Astrophysics Data System (ADS)

    Milanic, Matija; Paluchowski, Lukasz A.; Randeberg, Lise L.

    2015-09-01

    Rheumatoid arthritis (RA) is a disease that frequently leads to joint destruction. It has a high incidence rate worldwide, and the disease significantly reduces patients' quality of life. Detecting and treating inflammatory arthritis before structural damage to the joint has occurred is known to be essential for preventing patient disability and pain. Existing diagnostic technologies are expensive, time consuming, and require trained personnel to collect and interpret data. Optical techniques might be a fast, noninvasive alternative. Hyperspectral imaging (HSI) is a noncontact optical technique which provides both spectral and spatial information in one measurement. In this study, the feasibility of HSI in arthritis diagnostics was explored by numerical simulations and optimal imaging parameters were identified. Hyperspectral reflectance and transmission images of RA and normal human joint models were simulated using the Monte Carlo method. The spectral range was 600 to 1100 nm. Characteristic spatial patterns for RA joints and two spectral windows with transmission were identified. The study demonstrated that transmittance images of human joints could be used as one parameter for discrimination between arthritic and unaffected joints. The presented work shows that HSI is a promising imaging modality for the diagnostics and follow-up monitoring of arthritis in small joints.

  13. Hyperspectral imaging of microalgae using two-photon excitation.

    SciTech Connect

    Sinclair, Michael B.; Melgaard, David Kennett; Reichardt, Thomas A.; Timlin, Jerilyn Ann; Garcia, Omar Fidel; Luk, Ting Shan; Jones, Howland D. T.; Collins, Aaron M.

    2010-10-01

    A considerable amount research is being conducted on microalgae, since microalgae are becoming a promising source of renewable energy. Most of this research is centered on lipid production in microalgae because microalgae produce triacylglycerol which is ideal for biodiesel fuels. Although we are interested in research to increase lipid production in algae, we are also interested in research to sustain healthy algal cultures in large scale biomass production farms or facilities. The early detection of fluctuations in algal health, productivity, and invasive predators must be developed to ensure that algae are an efficient and cost-effective source of biofuel. Therefore we are developing technologies to monitor the health of algae using spectroscopic measurements in the field. To do this, we have proposed to spectroscopically monitor large algal cultivations using LIDAR (Light Detection And Ranging) remote sensing technology. Before we can deploy this type of technology, we must first characterize the spectral bio-signatures that are related to algal health. Recently, we have adapted our confocal hyperspectral imaging microscope at Sandia to have two-photon excitation capabilities using a chameleon tunable laser. We are using this microscope to understand the spectroscopic signatures necessary to characterize microalgae at the cellular level prior to using these signatures to classify the health of bulk samples, with the eventual goal of using of LIDAR to monitor large scale ponds and raceways. By imaging algal cultures using a tunable laser to excite at several different wavelengths we will be able to select the optimal excitation/emission wavelengths needed to characterize algal cultures. To analyze the hyperspectral images generated from this two-photon microscope, we are using Multivariate Curve Resolution (MCR) algorithms to extract the spectral signatures and their associated relative intensities from the data. For this presentation, I will show our two

  14. Object detection in rural areas using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Ozturk, Safak; Emre Esin, Yunus; Artan, Yusuf

    2015-10-01

    Object detection has gained considerable interest in remote sensing community with a broad range of applications including the remote monitoring of building development in rural areas. Many earlier studies on this task performed their analysis using either multispectral satellite imagery or color images obtained via an aerial vehicle. In recent years, hyperspectral imaging (HSI) has emerged as an alternative technique for remote monitoring of building developments. Unlike other imaging techniques, HSI provides a continuous spectral signature of the objects in the field of view (FOV) which facilitates the separation among different objects. In general, spectral signature similarity between objects often causes a significant amount of false alarm (FA) rate that adversely effects the overall accuracy of these systems. In order to reduce the high rate of FA posed by the pixel-wise classification, we propose a novel rural building detection method that utilizes both spatial information and spectral signature of the pixels. Proposed technique consists of three parts; a spectral signature classifier, watershed based superpixel map and an oriented-gradient filters based object detector. In our analysis, we have evaluated the performance of proposed approach using hyperspectral image dataset obtained at various elevation levels, namely 500 meters and 3000 meters. NEO HySpex VNIR-1800 camera is used for 182 band hyperspectral data acquisition. First 155 band is used due to the atmospheric effects on the last bands. Performance comparison between the proposed technique and the pixel-wise spectral classifier indicates a reduction in sensitivity rate but a notable increase in specificity and overall accuracy rates. Proposed method yields sensitivity, specificity, accuracy rate of 0.690, 0.997 and 0.992, respectively, whereas pixel-wise classification yields sensitivity, specificity, and accuracy rate of 0.758, 0.983, 0.977, respectively. Note that the sensitivity reduction is

  15. Multiclass feature learning for hyperspectral image classification: Sparse and hierarchical solutions

    NASA Astrophysics Data System (ADS)

    Tuia, Devis; Flamary, Rémi; Courty, Nicolas

    2015-07-01

    In this paper, we tackle the question of discovering an effective set of spatial filters to solve hyperspectral classification problems. Instead of fixing a priori the filters and their parameters using expert knowledge, we let the model find them within random draws in the (possibly infinite) space of possible filters. We define an active set feature learner that includes in the model only features that improve the classifier. To this end, we consider a fast and linear classifier, multiclass logistic classification, and show that with a good representation (the filters discovered), such a simple classifier can reach at least state of the art performances. We apply the proposed active set learner in four hyperspectral image classification problems, including agricultural and urban classification at different resolutions, as well as multimodal data. We also propose a hierarchical setting, which allows to generate more complex banks of features that can better describe the nonlinearities present in the data.

  16. Parallel optimization of pixel purity index algorithm for massive hyperspectral images in cloud computing environment

    NASA Astrophysics Data System (ADS)

    Chen, Yufeng; Wu, Zebin; Sun, Le; Wei, Zhihui; Li, Yonglong

    2016-04-01

    With the gradual increase in the spatial and spectral resolution of hyperspectral images, the size of image data becomes larger and larger, and the complexity of processing algorithms is growing, which poses a big challenge to efficient massive hyperspectral image processing. Cloud computing technologies distribute computing tasks to a large number of computing resources for handling large data sets without the limitation of memory and computing resource of a single machine. This paper proposes a parallel pixel purity index (PPI) algorithm for unmixing massive hyperspectral images based on a MapReduce programming model for the first time in the literature. According to the characteristics of hyperspectral images, we describe the design principle of the algorithm, illustrate the main cloud unmixing processes of PPI, and analyze the time complexity of serial and parallel algorithms. Experimental results demonstrate that the parallel implementation of the PPI algorithm on the cloud can effectively process big hyperspectral data and accelerate the algorithm.

  17. [Impact analysis of atmospheric state for target detection in hyperspectral radiance image].

    PubMed

    Zhang, Bing; Sha, Jian-jun; Wang, Xiang-wei; Gao, Lian-ru

    2012-08-01

    Target detection based on hyperspectral radiance images can improve data processing efficiency to meet the requirements of real-time processing. However, the spectral radiance acquired by the remote sensor will be affected by the atmosphere. In the present paper, hyperspectral imaging process is simulated to analyze the effects of the changes in atmospheric state on target detection in hyperspectral radiance image. The results show that hyperspectral radiance image can be directly used for target detection, different atmospheric states have little impacts on the RXD detection, whereas the MF detection is dependent on the accuracy of the input spectrum, and good results can only be obtained by the MF detector when the atmospheric states are similar between the radiance spectrum of the target to be detected and the simulated hyperspectral image. PMID:23156749

  18. Rapid hyperspectral imaging in the mid-infrared

    NASA Astrophysics Data System (ADS)

    Kröger, N.; Egl, A.; Engel, M.; Gretz, N.; Haase, K.; Herpich, I.; Neudecker, S.; Pucci, A.; Schönhals, A.; Petrich, W.

    2014-03-01

    Despite the successes of mid-infrared hyperspectral imaging in a research environment, progress in the migration of technology into the day-to-day clinical application is slow. Clinical acceptance may be improved if the spectroscopy would be faster and the infrared microscopes available at lower cost. Here we present first results of a fast, multi-scale mid-infrared microscopy setup which allows for the investigation of 10.6×11.7 mm2 and 2.8×3.1mm2 fields of view with a resolution of 23.0+/-3.5 μm and 9.4+/-1.8 μm, respectively. Tunable quantum cascade lasers in the wavenumber ranges of 1030-1090 cm-1 and 1160-1320 cm-1 serve as light sources. A vapor cell is used as a frequency reference during the rapid scanning. As far as the imaging is concerned, it is the high spectral power density of the quantum cascade laser which enables the use of a microbolometer array while still obtaining reasonable signal-to-noise ratios on each pixel. Hyperspectral images are taken in times which can be as low as 52s for the overall image acquisition including referencing.

  19. Radiometric Characterization of Hyperspectral Imagers using Multispectral Sensors

    NASA Technical Reports Server (NTRS)

    McCorkel, Joel; Kurt, Thome; Leisso, Nathan; Anderson, Nikolaus; Czapla-Myers, Jeff

    2009-01-01

    The Remote Sensing Group (RSG) at the University of Arizona has a long history of using ground-based test sites for the calibration of airborne and satellite based sensors. Often, ground-truth measurements at these test sites are not always successful due to weather and funding availability. Therefore, RSG has also automated ground instrument approaches and cross-calibration methods to verify the radiometric calibration of a sensor. The goal in the cross-calibration method is to transfer the calibration of a well-known sensor to that of a different sensor, This work studies the feasibility of determining the radiometric calibration of a hyperspectral imager using multispectral a imagery. The work relies on the Moderate Resolution Imaging Spectroradiometer (M0DIS) as a reference for the hyperspectral sensor Hyperion. Test sites used for comparisons are Railroad Valley in Nevada and a portion of the Libyan Desert in North Africa. Hyperion bands are compared to MODIS by band averaging Hyperion's high spectral resolution data with the relative spectral response of M0DlS. The results compare cross-calibration scenarios that differ in image acquisition coincidence, test site used for the calibration, and reference sensor. Cross-calibration results are presented that show agreement between the use of coincident and non-coincident image pairs within 2% in most brands as well as similar agreement between results that employ the different MODIS sensors as a reference.

  20. Improved MCA-TV algorithm for interference hyperspectral image decomposition

    NASA Astrophysics Data System (ADS)

    Wen, Jia; Zhao, Junsuo; Cailing, Wang

    2015-12-01

    The technology of interference hyperspectral imaging, which can get the spectral and spatial information of the observed targets, is a very powerful technology in the field of remote sensing. Due to the special imaging principle, there are many position-fixed interference fringes in each frame of the interference hyperspectral image (IHI) data. This characteristic will affect the result of compressed sensing theory and traditional compression algorithms used on IHI data. According to this characteristic of the IHI data, morphological component analysis (MCA) is adopted to separate the interference fringes layers and the background layers of the LSMIS (Large Spatially Modulated Interference Spectral Image) data, and an improved MCA and Total Variation (TV) combined algorithm is proposed in this paper. An update mode of the threshold in traditional MCA is proposed, and the traditional TV algorithm is also improved according to the unidirectional characteristic of the interference fringes in IHI data. The experimental results prove that the proposed improved MCA-TV (IMT) algorithm can get better results than the traditional MCA, and also can meet the convergence conditions much faster than the traditional MCA.

  1. Accurate reconstruction of hyperspectral images from compressive sensing measurements

    NASA Astrophysics Data System (ADS)

    Greer, John B.; Flake, J. C.

    2013-05-01

    The emerging field of Compressive Sensing (CS) provides a new way to capture data by shifting the heaviest burden of data collection from the sensor to the computer on the user-end. This new means of sensing requires fewer measurements for a given amount of information than traditional sensors. We investigate the efficacy of CS for capturing HyperSpectral Imagery (HSI) remotely. We also introduce a new family of algorithms for constructing HSI from CS measurements with Split Bregman Iteration [Goldstein and Osher,2009]. These algorithms combine spatial Total Variation (TV) with smoothing in the spectral dimension. We examine models for three different CS sensors: the Coded Aperture Snapshot Spectral Imager-Single Disperser (CASSI-SD) [Wagadarikar et al.,2008] and Dual Disperser (CASSI-DD) [Gehm et al.,2007] cameras, and a hypothetical random sensing model closer to CS theory, but not necessarily implementable with existing technology. We simulate the capture of remotely sensed images by applying the sensor forward models to well-known HSI scenes - an AVIRIS image of Cuprite, Nevada and the HYMAP Urban image. To measure accuracy of the CS models, we compare the scenes constructed with our new algorithm to the original AVIRIS and HYMAP cubes. The results demonstrate the possibility of accurately sensing HSI remotely with significantly fewer measurements than standard hyperspectral cameras.

  2. Radiometric characterization of hyperspectral imagers using multispectral sensors

    NASA Astrophysics Data System (ADS)

    McCorkel, Joel; Thome, Kurt; Leisso, Nathan; Anderson, Nikolaus; Czapla-Myers, Jeff

    2009-08-01

    The Remote Sensing Group (RSG) at the University of Arizona has a long history of using ground-based test sites for the calibration of airborne and satellite based sensors. Often, ground-truth measurements at these tests sites are not always successful due to weather and funding availability. Therefore, RSG has also employed automated ground instrument approaches and cross-calibration methods to verify the radiometric calibration of a sensor. The goal in the cross-calibration method is to transfer the calibration of a well-known sensor to that of a different sensor. This work studies the feasibility of determining the radiometric calibration of a hyperspectral imager using multispectral imagery. The work relies on the Moderate Resolution Imaging Spectroradiometer (MODIS) as a reference for the hyperspectral sensor Hyperion. Test sites used for comparisons are Railroad Valley in Nevada and a portion of the Libyan Desert in North Africa. Hyperion bands are compared to MODIS by band averaging Hyperion's high spectral resolution data with the relative spectral response of MODIS. The results compare cross-calibration scenarios that differ in image acquisition coincidence, test site used for the calibration, and reference sensor. Cross-calibration results are presented that show agreement between the use of coincident and non-coincident image pairs within 2% in most bands as well as similar agreement between results that employ the different MODIS sensors as a reference.

  3. Security inspection in ports by anomaly detection using hyperspectral imaging technology

    NASA Astrophysics Data System (ADS)

    Rivera, Javier; Valverde, Fernando; Saldaña, Manuel; Manian, Vidya

    2013-05-01

    Applying hyperspectral imaging technology in port security is crucial for the detection of possible threats or illegal activities. One of the most common problems that cargo suffers is tampering. This represents a danger to society because it creates a channel to smuggle illegal and hazardous products. If a cargo is altered, security inspections on that cargo should contain anomalies that reveal the nature of the tampering. Hyperspectral images can detect anomalies by gathering information through multiple electromagnetic bands. The spectrums extracted from these bands can be used to detect surface anomalies from different materials. Based on this technology, a scenario was built in which a hyperspectral camera was used to inspect the cargo for any surface anomalies and a user interface shows the results. The spectrum of items, altered by different materials that can be used to conceal illegal products, is analyzed and classified in order to provide information about the tampered cargo. The image is analyzed with a variety of techniques such as multiple features extracting algorithms, autonomous anomaly detection, and target spectrum detection. The results will be exported to a workstation or mobile device in order to show them in an easy -to-use interface. This process could enhance the current capabilities of security systems that are already implemented, providing a more complete approach to detect threats and illegal cargo.

  4. DLP hyperspectral imaging for surgical and clinical utility

    NASA Astrophysics Data System (ADS)

    Zuzak, Karel J.; Francis, Robert P.; Wehner, Eleanor F.; Smith, Jack; Litorja, Maritoni; Allen, David W.; Tracy, Chad; Cadeddu, Jeffrey; Livingston, Edward

    2009-02-01

    We describe a novel digital light processing, DLP hyperspectral imaging system for visualizing chemical composition of in vivo tissues during surgical procedures non-invasively and at near video rate. The novelty of the DLP hyperspectral imaging system resides in (1) its ability to conform light to rapidly sweep through a series of preprogrammed spectral illuminations as simple as a set of contiguous bandpasses to any number of complex spectra, and (2) processing the reflected spectroscopic image data using unique supervised and unsupervised chemometric methods that color encode molecular content of tissue at each image detector pixel providing an optical biopsy. Spectral illumination of tissue is accomplished utilizing a DLP® based spectral illuminator incorporating a series of bandpass spectra and measuring the reflectance image with a CCD array detector. Wavelength dependent images are post processed with a multivariate least squares analysis method using known reference spectra of oxy- and deoxyhemoglobin. Alternatively, illuminating with complex reference spectra reduces the number of spectral images required for generating chemically relevant images color encoded for relative percentage of oxyhemoglobin are collected and displayed in real time near-video rate, (3 to 4) frames per second (fps). As a proof of principle application, a kidney of an anesthetized pig was imaged before and after renal vasculature occlusion showing the clamped kidney to be 61% of the unclamped kidney percentage of oxyhemoglobin. Using the "3-Shot" spectral illumination method and gathering data at (3 to 4) fps shows a non-linear exponential de-oxygenation of hemoglobin reaching steady state within 30 seconds post occlusion.

  5. Material characterization using a hyperspectral infrared imaging spectrometer

    SciTech Connect

    Aimonetti, W D; Bixler, J V; Roberts, R S

    1998-10-30

    Fourier transform spectroscopy has found application in many areas including chemometrics, biomedical and biochemical studies, and atmospheric chemistry. This paper describes an investigation into the application of the LLNL Hyperspectral Infrared Imaging Spectrometer (HIRIS) to the non-destructive evaluation of man-made and natural materials. We begin by describing the HIRIS system and the objects studied in the investigation. Next, we describe the technique used to collect the hyperspec- tral imagery, and discuss the processing required to transform the data into usable form. We then describe a technique to analyze the data, and provide some preliminary results.

  6. Application of novel hyperspectral imaging technologies in combat casualty care

    NASA Astrophysics Data System (ADS)

    Cancio, Leopoldo C.

    2010-02-01

    Novel hyperspectral imaging (HSI) methods may play several important roles in Combat Casualty Care: (1) HSI of the skin may provide spatial data on hemoglobin saturation of oxygen, as a "window" into perfusion during shock. (2) HSI or similar technology could be incorporated into closed-loop, feedback-controlled resuscitation systems. (3) HSI may provide information about tissue viability and/or wound infection. (4) HSI in the near-infrared range may provide information on the tissue water content--greatly affected, e.g., by fluid resuscitation. Thus, further refinements in the speed and size of HSI systems are sought to make these capabilities available on the battlefield.

  7. Classification of fecal contamination on leafy greens by hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Yang, Chun-Chieh; Jun, Won; Kim, Moon S.; Chao, Kaunglin; Kang, Sukwon; Chan, Diane E.; Lefcourt, Alan

    2010-04-01

    This paper reported the development of hyperspectral fluorescence imaging system using ultraviolet-A excitation (320-400 nm) for detection of bovine fecal contaminants on the abaxial and adaxial surfaces of romaine lettuce and baby spinach leaves. Six spots of fecal contamination were applied to each of 40 lettuce and 40 spinach leaves. In this study, the wavebands at 666 nm and 680 nm were selected by the correlation analysis. The two-band ratio, 666 nm / 680 nm, of fluorescence intensity was used to differentiate the contaminated spots from uncontaminated leaf area. The proposed method could accurately detect all of the contaminated spots.

  8. New hyperspectral difference water index for the extraction of urban water bodies by the use of airborne hyperspectral images

    NASA Astrophysics Data System (ADS)

    Xie, Huan; Luo, Xin; Xu, Xiong; Tong, Xiaohua; Jin, Yanmin; Pan, Haiyan; Zhou, Bingzhong

    2014-01-01

    Extracting surface land-cover types and analyzing changes are among the most common applications of remote sensing. One of the most basic tasks is to identify and map surface water boundaries. Spectral water indexes have been successfully used in the extraction of water bodies in multispectral images. However, directly applying a water index method to hyperspectral images disregards the abundant spectral information and involves difficulty in selecting appropriate spectral bands. It is also a challenge for a spectral water index to distinguish water from shadowed regions. The purpose of this study is therefore to develop an index that is suitable for water extraction by the use of hyperspectral images, and with the capability to mitigate the effects of shadow and low-albedo surfaces, especially in urban areas. Thus, we introduce a new hyperspectral difference water index (HDWI) to improve the water classification accuracy in areas that include shadow over water, shadow over other ground surfaces, and low-albedo ground surfaces. We tested the new method using PHI-2, HyMAP, and ROSIS hyperspectral images of Shanghai, Munich, and Pavia. The performance of the water index was compared with the normalized difference water index (NDWI) and the Mahalanobis distance classifier (MDC). With all three test images, the accuracy of HDWI was significantly higher than that of NDWI and MDC. Therefore, HDWI can be used for extracting water with a high degree of accuracy, especially in urban areas, where shadow caused by high buildings is an important source of classification error.

  9. Melanoma detection using smartphone and multimode hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    MacKinnon, Nicholas; Vasefi, Fartash; Booth, Nicholas; Farkas, Daniel L.

    2016-04-01

    This project's goal is to determine how to effectively implement a technology continuum from a low cost, remotely deployable imaging device to a more sophisticated multimode imaging system within a standard clinical practice. In this work a smartphone is used in conjunction with an optical attachment to capture cross-polarized and collinear color images of a nevus that are analyzed to quantify chromophore distribution. The nevus is also imaged by a multimode hyperspectral system, our proprietary SkinSpect™ device. Relative accuracy and biological plausibility of the two systems algorithms are compared to assess aspects of feasibility of in-home or primary care practitioner smartphone screening prior to rigorous clinical analysis via the SkinSpect.

  10. Advanced hyperspectral video imaging system using Amici prism.

    PubMed

    Feng, Jiao; Fang, Xiaojing; Cao, Xun; Ma, Chenguang; Dai, Qionghai; Zhu, Hongbo; Wang, Yongjin

    2014-08-11

    In this paper, we propose an advanced hyperspectral video imaging system (AHVIS), which consists of an objective lens, an occlusion mask, a relay lens, an Amici prism and two cameras. An RGB camera is used for spatial reading and a gray scale camera is used for measuring the scene with spectral information. The objective lens collects more light energy from the observed scene and images the scene on an occlusion mask, which subsamples the image of the observed scene. Then, the subsampled image is sent to the gray scale camera through the relay lens and the Amici prism. The Amici prism that is used to realize spectral dispersion along the optical path reduces optical distortions and offers direct view of the scene. The main advantages of the proposed system are improved light throughput and less optical distortion. Furthermore, the presented configuration is more compact, robust and practicable. PMID:25321019

  11. Hyperspectral imaging in quality control of inkjet printed personalised dosage forms.

    PubMed

    Vakili, Hossein; Kolakovic, Ruzica; Genina, Natalja; Marmion, Mathieu; Salo, Harri; Ihalainen, Petri; Peltonen, Jouko; Sandler, Niklas

    2015-04-10

    The aim of the study was to investigate applicability of near infra-red (NIR) hyperspectral imaging technique in quality control of printed personalised dosage forms. Inkjet printing technology was utilized to fabricate escalating doses of an active pharmaceutical ingredient (API). A solution containing anhydrous theophylline as the model drug was developed as a printable formulation. Single units solid dosage forms (SDFs) were prepared by jetting the solution onto 1 cm × 1 cm areas on carrier substrate with multiple printing passes. It was found that the number of printing passes was in excellent correlation (R(2)=0.9994) with the amount of the dispensed drug (μg cm(-2)) based on the UV calibration plot. The API dose escalation was approximately 7.5 μg cm(-2) for each printing pass concluding that inkjet printing technology can optimally provide solutions to accurate deposition of active substances with a potential for personalized dosing. Principal component analysis (PCA) was carried out in order to visualize the trends in the hyperspectral data. Subsequently, a quantitative partial least squares (PLS) regression model was created. NIR hyperspectral imaging proved (R(2)=0.9767) to be a reliable, rapid and non-destructive method to optimize quality control of these planar printed dosage forms. PMID:25527212

  12. Superpixel-based spectral classification for the detection of head and neck cancer with hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Chung, Hyunkoo; Lu, Guolan; Tian, Zhiqiang; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

    2016-03-01

    Hyperspectral imaging (HSI) is an emerging imaging modality for medical applications. HSI acquires two dimensional images at various wavelengths. The combination of both spectral and spatial information provides quantitative information for cancer detection and diagnosis. This paper proposes using superpixels, principal component analysis (PCA), and support vector machine (SVM) to distinguish regions of tumor from healthy tissue. The classification method uses 2 principal components decomposed from hyperspectral images and obtains an average sensitivity of 93% and an average specificity of 85% for 11 mice. The hyperspectral imaging technology and classification method can have various applications in cancer research and management.

  13. On the response function separability of hyperspectral imaging systems

    NASA Astrophysics Data System (ADS)

    Jemec, Jurij; Pernuš, Franjo; Likar, Boštjan; Bürmen, Miran

    2015-05-01

    Hyperspectral imaging systems effectively collect information across the spectral and two spatial dimensions by employing three main components: the front lens, the light-diffraction element and a camera. Imperfections in these components introduce spectral and spatial dependent distortions in the recorded hyperspectral image. These can be characterized by a 3D response function that is subsequently used to remove distortions and enhance the resolution of the recorded images by deconvolution. The majority of existing characterization methods assume spatial and spectral separability of the 3D response function. In this way, the complex problem of 3D response function characterization is reduced to independent characterizations of the three orthogonal response function components. However, if the 3D response function is non-separable, such characterization can lead to poor response function estimates, and hence inaccurate and distorted results of the subsequent deconvolution-based calibration and image enhancement. In this paper, we evaluate the influence of the spatial response function non-separability on the results of the calibration by deconvolution. For this purpose, a novel procedure for direct measurement of the 2D spatial response function is proposed along with a quantitative measure of the spatial response function non-separability. The quality of deconvolved images is assessed in terms of full width at half maximum (FWHM) and step edge overshoot magnitude observed in the deconvolved images of slanted edges, images of biological slides, and 1951 USAF resolution test chart. Results show that there are cases, when nonseparability of the system response function is significant and should be considered by the deconvolution-based calibration and image enhancement methods.

  14. Hyperspectral imaging of UVR effects on fungal spectrum

    NASA Astrophysics Data System (ADS)

    Hruska, Zuzana; Yao, Haibo; DiCrispino, Kevin; Brabham, Kori; Lewis, David; Beach, Jim; Brown, Robert L.; Cleveland, Thomas E.

    2005-08-01

    The present report evaluated ultraviolet radiation (UVR) effects on the spectral signature of mycotoxin producing fungus Aspergillus flavus (A. flavus). Ultraviolet radiation has long been used to reduce microbe contamination and to inactivate mold spores. In view of the known effects of UVR on microorganisms, and because certain spectral bands in the signature of some fungi may be in the UV range, it is important to know the maximum acceptable limit of UVR exposure that does not significantly alter the fungal spectral signature and affect detection accuracy. A visible-near-infrared (VNIR) hyperspectral imaging system using focal plane pushbroom scanning for high spatial and spectral resolution imaging was utilized to detect any changes. A. flavus cultures were grown for 5 days and imaged after intermittent or continuous UVR treatment. The intermittent group was treated at 1-minute intervals for 10 minutes, and VNIR images were taken after each UVR treatment. The continuous group was irradiated for 10 minutes and imaged before and after treatment. A control sample group did not undergo UVR treatment, but was also imaged at 1-minute intervals for 10 minutes in the same manner as the intermittent group. Before and after UVR treatment, mean fungal sample reflectance was obtained through spatial subset of the image along with standard deviation and pre- and post-treatment reflectance was compared for each sample. Results show significant difference between the reflectances of treated and control A. flavus cultures after 10 min of UV radiation. Aditionally, the results demonstrate that even lethal doses of UVR do not immediately affect the spectral signature of A. flavus cultures suggesting that the excitation UV light source used in the present experiment may be safe to use with the UV hyperspectral imaging system when exposure time falls below 10 min.

  15. Construction of a small and lightweight hyperspectral imaging system

    NASA Astrophysics Data System (ADS)

    Vogel, Britta; Hünniger, Dirk; Bastian, Georg

    2014-05-01

    The analysis of the reflected sunlight offers great opportunity to gain information about the environment, including vegetation and soil. In the case of plants the wavelength ratio of the reflected light usually undergoes a change if the state of growth or state of health changes. So the measurement of the reflected light allows drawing conclusions about the state of, amongst others, vegetation. Using a hyperspectral imaging system for data acquisition leads to a large dataset, which can be evaluated with respect to several different questions to obtain various information by one measurement. Based on commercially available plain optical components we developed a small and lightweight hyperspectral imaging system within the INTERREG IV A-Project SMART INSPECTORS. The project SMART INSPECTORS [Smart Aerial Test Rigs with Infrared Spectrometers and Radar] deals with the fusion of airborne visible and infrared imaging remote sensing instruments and wireless sensor networks for precision agriculture and environmental research. A high performance camera was required in terms of good signal, good wavelength resolution and good spatial resolution, while severe constraints of size, proportions and mass had to be met due to the intended use on small unmanned aerial vehicles. The detector was chosen to operate without additional cooling. The refractive and focusing optical components were identified by supporting works with an optical raytracing software and a self-developed program. We present details of design and construction of our camera system, test results to confirm the optical simulation predictions as well as our first measurements.

  16. Calibration methodology and performance characterization of a polarimetric hyperspectral imager

    NASA Astrophysics Data System (ADS)

    Holder, Joel G.; Martin, Jacob A.; Pitz, Jeremey; Pezzaniti, Joseph L.; Gross, Kevin C.

    2014-05-01

    Polarimetric hyperspectral imaging (P-HSI) has the potential to improve target detection, material identification, and background characterization over conventional hyperspectral imaging and polarimetric imaging. To fully exploit the spectro-polarimetric signatures captured by such an instrument, a careful calibration process is required to remove the spectrally- and polarimetrically-dependent system response (gain). Calibration of instruments operating in the long-wave infrared (LWIR, 8μm to 12 μm) is further complicated by the polarized spectral radiation generated within the instrument (offset). This paper presents a calibration methodology developed for a LWIR Telops Hyper-Cam modified for polarimetry by replacing the entrance window with a rotatable holographic wire-grid polarizer (4000 line/mm, ZnSe substrate, 350:1 extinction ratio). A standard Fourier-transform spectrometer (FTS) spectro-radiometric calibration is modified to include a Mueller-matrix approach to account for polarized transmission through and polarized selfemission from each optical interface. It is demonstrated that under the ideal polarizer assumption, two distinct blackbody measurements at polarizer angles of 0°, 45°, 90°, and 135° are sufficient to calibrate the system for apparent degree-of-linear-polarization (DoLP) measurements. Noise-equivalent s1, s2, and DoLP are quantified using a wide-area blackbody. A polarization-state generator is used to determine the Mueller deviation matrix. Finally, a realistic scene involving buildings, cars, sky radiance, and natural vegetation is presented.

  17. Hyperspectral Image Target Detection Improvement Based on Total Variation.

    PubMed

    Yang, Shuo; Shi, Zhenwei

    2016-05-01

    For the hyperspectral target detection, the neighbors of a target pixel are very likely to be target pixels, and those of a background pixel are very likely to be background pixels. In order to utilize this spatial homogeneity or smoothness, based on total variation (TV), we propose a novel supervised target detection algorithm which uses a single target spectrum as the prior knowledge. TV can make the image smooth, and has been widely used in image denoising and restoration. The proposed algorithm uses TV to keep the spatial homogeneity or smoothness of the detection output. Meanwhile, a constraint is used to guarantee the spectral signature of the target unsuppressed. The final formulated detection model is an ℓ1-norm convex optimization problem. The split Bregman algorithm is used to solve our optimization problem, as it can solve the ℓ1-norm optimization problem efficiently. Two synthetic and two real hyperspectral images are used to do experiments. The experimental results demonstrate that the proposed algorithm outperforms the other algorithms for the experimental data sets. The experimental results also show that even when the target occupies only one pixel, the proposed algorithm can still obtain good results. This is because in such a case, the background is kept smooth, but at the same time, the algorithm allows for sharp edges in the detection output. PMID:27019489

  18. Oil Adulteration Identification by Hyperspectral Imaging Using QHM and ICA.

    PubMed

    Han, Zhongzhi; Wan, Jianhua; Deng, Limiao; Liu, Kangwei

    2016-01-01

    To investigate the feasibility of identification of qualified and adulterated oil product using hyperspectral imaging(HIS) technique, a novel feature set based on quantized histogram matrix (QHM) and feature selection method using improved kernel independent component analysis (iKICA) is proposed for HSI. We use UV and Halogen excitations in this study. Region of interest(ROI) of hyperspectral images of 256 oil samples from four varieties are obtained within the spectral region of 400-720nm. Radiation indexes extracted from each ROI are used as feature vectors. These indexes are individual band radiation index (RI), difference of consecutive spectral band radiation index (DRI), ratio of consecutive spectral band radiation index (RRI) and normalized DRI (NDRI). Another set of features called quantized histogram matrix (QHM) are extracted by applying quantization on the image histogram from these features. Based on these feature sets, improved kernel independent component analysis (iKICA) is used to select significant features. For comparison, algorithms such as plus L reduce R (plusLrR), Fisher, multidimensional scaling (MDS), independent component analysis (ICA), and principle component analysis (PCA) are also used to select the most significant wavelengths or features. Support vector machine (SVM) is used as the classifier. Experimental results show that the proposed methods are able to obtain robust and better classification performance with fewer number of spectral bands and simplify the design of computer vision systems. PMID:26820311

  19. Hyperspectral pixel classification from coded-aperture compressive imaging

    NASA Astrophysics Data System (ADS)

    Ramirez, Ana; Arce, Gonzalo R.; Sadler, Brian M.

    2012-06-01

    This paper describes a new approach and its associated theoretical performance guarantees for supervised hyperspectral image classification from compressive measurements obtained by a Coded Aperture Snapshot Spectral Imaging System (CASSI). In one snapshot, the two-dimensional focal plane array (FPA) in the CASSI system captures the coded and spectrally dispersed source field of a three-dimensional data cube. Multiple snapshots are used to construct a set of compressive spectral measurements. The proposed approach is based on the concept that each pixel in the hyper-spectral image lies in a low-dimensional subspace obtained from the training samples, and thus it can be represented as a sparse linear combination of vectors in the given subspace. The sparse vector representing the test pixel is then recovered from the set of compressive spectral measurements and it is used to determine the class label of the test pixel. The theoretical performance bounds of the classifier exploit the distance preservation condition satisfied by the multiple shot CASSI system and depend on the number of measurements collected, code aperture pattern, and similarity between spectral signatures in the dictionary. Simulation experiments illustrate the performance of the proposed classification approach.

  20. Oil Adulteration Identification by Hyperspectral Imaging Using QHM and ICA

    PubMed Central

    Han, Zhongzhi; Wan, Jianhua; Deng, Limiao; Liu, Kangwei

    2016-01-01

    To investigate the feasibility of identification of qualified and adulterated oil product using hyperspectral imaging(HIS) technique, a novel feature set based on quantized histogram matrix (QHM) and feature selection method using improved kernel independent component analysis (iKICA) is proposed for HSI. We use UV and Halogen excitations in this study. Region of interest(ROI) of hyperspectral images of 256 oil samples from four varieties are obtained within the spectral region of 400–720nm. Radiation indexes extracted from each ROI are used as feature vectors. These indexes are individual band radiation index (RI), difference of consecutive spectral band radiation index (DRI), ratio of consecutive spectral band radiation index (RRI) and normalized DRI (NDRI). Another set of features called quantized histogram matrix (QHM) are extracted by applying quantization on the image histogram from these features. Based on these feature sets, improved kernel independent component analysis (iKICA) is used to select significant features. For comparison, algorithms such as plus L reduce R (plusLrR), Fisher, multidimensional scaling (MDS), independent component analysis (ICA), and principle component analysis (PCA) are also used to select the most significant wavelengths or features. Support vector machine (SVM) is used as the classifier. Experimental results show that the proposed methods are able to obtain robust and better classification performance with fewer number of spectral bands and simplify the design of computer vision systems. PMID:26820311

  1. A novel highly parallel algorithm for linearly unmixing hyperspectral images

    NASA Astrophysics Data System (ADS)

    Guerra, Raúl; López, Sebastián.; Callico, Gustavo M.; López, Jose F.; Sarmiento, Roberto

    2014-10-01

    Endmember extraction and abundances calculation represent critical steps within the process of linearly unmixing a given hyperspectral image because of two main reasons. The first one is due to the need of computing a set of accurate endmembers in order to further obtain confident abundance maps. The second one refers to the huge amount of operations involved in these time-consuming processes. This work proposes an algorithm to estimate the endmembers of a hyperspectral image under analysis and its abundances at the same time. The main advantage of this algorithm is its high parallelization degree and the mathematical simplicity of the operations implemented. This algorithm estimates the endmembers as virtual pixels. In particular, the proposed algorithm performs the descent gradient method to iteratively refine the endmembers and the abundances, reducing the mean square error, according with the linear unmixing model. Some mathematical restrictions must be added so the method converges in a unique and realistic solution. According with the algorithm nature, these restrictions can be easily implemented. The results obtained with synthetic images demonstrate the well behavior of the algorithm proposed. Moreover, the results obtained with the well-known Cuprite dataset also corroborate the benefits of our proposal.

  2. SETA-Hyperspectral Imaging Spectrometer for Marco Polo mission.

    NASA Astrophysics Data System (ADS)

    de Sanctis, M. Cristina; Filacchione, Gianrico; Capaccioni, Fabrizio; Piccioni, Giuseppe; Ammannito, Eleonora; Capria, M. Teresa; Coradini, Angioletta; Migliorini, Alessandra; Battistelli, Enrico; Preti, Giampaolo

    2010-05-01

    The Marco Polo NEO sample return M-class mission has been selected for assessment study within the ESA Cosmic Vision 2015-2025 program. The Marco Polo mission proposes to do a sample return mission to Near Earth Asteroid. With this mission we have the opportunity to return for study in Earth-based laboratories a direct sample of the earliest record of how our solar system formed. The landing site and sample selection will be the most important scientific decision to make during the course of the entire mission. The imaging spectrometer is a key instrument being capable to characterize the mineralogical composition of the entire asteroid and to analyze the of the landing site and the returned sample in its own native environment. SETA is a Hyperspectral Imaging Spectrometer able to perform imaging spectroscopy in the spectral range 400-3300 nm for a complete mapping of the target in order to characterize the mineral properties of the surface. The spectral sampling is of at least 20 nm and the spatial resolution of the order of meter. SETA shall be able to return a detailed determination of the mineralogical composition for the different geologic units as well as the overall surface mineralogy with a spatial resolution of the order of few meters. These compositional characterizations involve the analysis of spectral parameters that are diagnostic of the presence and composition of various mineral species and materials that may be present on the target body. Most of the interesting minerals have electronic and vibrational absorption features in their VIS-NIR reflectance spectra. The SETA design is based on a pushbroom imaging spectrometer operating in the 400-3300 nm range, using a 2D array HgCdTe detector. This kind of instrument allows a simultaneous measurement of a full spectrum taken across the field of view defined by the slit's axis (samples). The second direction (lines) of the hyperspectral image shall be obtained by using the relative motion of the orbiter

  3. New Method for Calibration for Hyperspectral Pushbroom Imaging Systems

    NASA Technical Reports Server (NTRS)

    Ryan, Robert; Olive, Dan; ONeal, Duane; Schere, Chris; Nixon, Thomas; May, Chengye; Ryan, Jim; Stanley, Tom; Witcher, Kern

    1999-01-01

    A new, easy-to-implement approach for achieving highly accurate spectral and radiometric calibration of array-based, hyperspectral pushbroom imagers is presented in this paper. The equivalence of the plane of the exit port of an integrating sphere to a Lambertian surface is utilized to provide a field-filling radiance source for the imager. Several different continuous wave lasers of various wavelengths and a quartz-tungsten-halogen lamp internally illuminate the sphere. The imager is positioned to "stare" into the port, and the resultant data cube is analyzed to determine wavelength calibrations, spectral widths of channels, radiometric characteristics, and signal-to-noise ratio, as well as an estimate of signal-to-noise performance in the field. The "smile" (geometric distortion of spectra) of the system can be quickly ascertained using this method. As the price and availability of solid state laser sources improve, this technique could gain wide acceptance.

  4. Methods for gas detection using stationary hyperspectral imaging sensors

    DOEpatents

    Conger, James L.; Henderson, John R.

    2012-04-24

    According to one embodiment, a method comprises producing a first hyperspectral imaging (HSI) data cube of a location at a first time using data from a HSI sensor; producing a second HSI data cube of the same location at a second time using data from the HSI sensor; subtracting on a pixel-by-pixel basis the second HSI data cube from the first HSI data cube to produce a raw difference cube; calibrating the raw difference cube to produce a calibrated raw difference cube; selecting at least one desired spectral band based on a gas of interest; producing a detection image based on the at least one selected spectral band and the calibrated raw difference cube; examining the detection image to determine presence of the gas of interest; and outputting a result of the examination. Other methods, systems, and computer program products for detecting the presence of a gas are also described.

  5. Hyperspectral data collections with the new wedge imaging spectrometer

    SciTech Connect

    Jeter, J.W.; Hartshorne, R.; Thunen, J.G.

    1996-11-01

    The Wedge Imaging Spectrometer (WIS) applies a unique technology to hyperspectral imaging systems, allowing flexibility and high performance in a very compact package. This innovation is based on the use of a linear spectral wedge filter mated directly to an area detector array, avoiding the use of bulky and complex optics required for imaging spectrometers based on gratings or prism concepts. The technology was realized in an earlier flight demonstration system as previously reported. Second generation VNIR and SWIR instruments have now been developed, each with two filters whose spectral bandwidths are optimized for specific spectral features. The SWIR instrument can be extended to operate in the 3-5 PM mid-wave spectral region. The new instrument is currently completing its integration and test phase. Preliminary results indicate excellent performance potential for a wide range of applications. 2 figs., 1 tab.

  6. Hyperspectral imaging applied to medical diagnoses and food safety

    NASA Astrophysics Data System (ADS)

    Carrasco, Oscar; Gomez, Richard B.; Chainani, Arun; Roper, William E.

    2003-08-01

    This paper analyzes the feasibility and performance of HSI systems for medical diagnosis as well as for food safety. Illness prevention and early disease detection are key elements for maintaining good health. Health care practitioners worldwide rely on innovative electronic devices to accurately identify disease. Hyperspectral imaging (HSI) is an emerging technique that may provide a less invasive procedure than conventional diagnostic imaging. By analyzing reflected and fluorescent light applied to the human body, a HSI system serves as a diagnostic tool as well as a method for evaluating the effectiveness of applied therapies. The safe supply and production of food is also of paramount importance to public health illness prevention. Although this paper will focus on imaging and spectroscopy in food inspection procedures -- the detection of contaminated food sources -- to ensure food quality, HSI also shows promise in detecting pesticide levels in food production (agriculture.)

  7. Performance results of pixel co-registered VisNIR-SWIR hyperspectral imaging system

    NASA Astrophysics Data System (ADS)

    Wong, Kwok-Keung

    2015-06-01

    The primary application of hyperspectral imaging is to classify/quantify objects/materials in the scene based on their spectral signatures. The spectral features that are useful can sometimes fall outside the spectral range of a single hyperspectral imager which is usually limited by the spectral response range of the sensor material of the focal plane array within the imager. For these wide spectrum applications, some users are combining data from two (or more) hyperspectral imaging systems. Aside from the optical alignment, size and synchronization issues involved in such a setup, the process of pixel co-registration, i.e. geometrically transforming data from the 2 hyperspectral imaging systems to overlay one another is tedious and complex. Headwall Photonics has integrated two of their off-the-shelf highly optimized hyperspectral imagers (Vis-NIR and SWIR) in an optically co-boresighted configuration together with a high performance data processor to produce a compact system which is easy to use and outputs wide spectrum pixel co-registered hyperspectral data. The process of pixel co-registration in this system is computationally very cheap enabling real-time wide-spectrum hyperspectral imaging applications. This paper presents actual imaging and performance data from these systems showing excellent pixel co-registration, sensitivity and spectral resolution.

  8. Investigation of Latent Traces Using Infrared Reflectance Hyperspectral Imaging

    NASA Astrophysics Data System (ADS)

    Schubert, Till; Wenzel, Susanne; Roscher, Ribana; Stachniss, Cyrill

    2016-06-01

    The detection of traces is a main task of forensics. Hyperspectral imaging is a potential method from which we expect to capture more fluorescence effects than with common forensic light sources. This paper shows that the use of hyperspectral imaging is suited for the analysis of latent traces and extends the classical concept to the conservation of the crime scene for retrospective laboratory analysis. We examine specimen of blood, semen and saliva traces in several dilution steps, prepared on cardboard substrate. As our key result we successfully make latent traces visible up to dilution factor of 1:8000. We can attribute most of the detectability to interference of electromagnetic light with the water content of the traces in the shortwave infrared region of the spectrum. In a classification task we use several dimensionality reduction methods (PCA and LDA) in combination with a Maximum Likelihood classifier, assuming normally distributed data. Further, we use Random Forest as a competitive approach. The classifiers retrieve the exact positions of labelled trace preparation up to highest dilution and determine posterior probabilities. By modelling the classification task with a Markov Random Field we are able to integrate prior information about the spatial relation of neighboured pixel labels.

  9. Online monitoring of red meat color using hyperspectral imaging.

    PubMed

    Kamruzzaman, Mohammed; Makino, Yoshio; Oshita, Seiichi

    2016-06-01

    A hyperspectral imaging system in the spectral range of 400-1000 nm was tested to develop an online monitoring system for red meat (beef, lamb, and pork) color in the meat industry. Instead of selecting different sets of important wavelengths for beef, lamb, and pork, a set of feature wavelengths were selected using the successive projection algorithm for red meat colors (L*, a*, b) for convenient industrial application. Only six wavelengths (450, 460, 600, 620, 820, and 980 nm) were further chosen as predictive feature wavelengths for predicting L*, a*, and b* in red meat. Multiple linear regression models were then developed and predicted L*, a*, and b* with coefficients of determination (R(2)p) of 0.97, 0.84, and 0.82, and root mean square error of prediction of 1.72, 1.73, and 1.35, respectively. Finally, distribution maps of meat surface color were generated. The results indicated that hyperspectral imaging has the potential to be used for rapid assessment of meat color. PMID:26874594

  10. Classification of oat and groat kernels using NIR hyperspectral imaging.

    PubMed

    Serranti, Silvia; Cesare, Daniela; Marini, Federico; Bonifazi, Giuseppe

    2013-01-15

    An innovative procedure to classify oat and groat kernels based on coupling hyperspectral imaging (HSI) in the near infrared (NIR) range (1006-1650 nm) and chemometrics was designed, developed and validated. According to market requirements, the amount of groat, that is the hull-less oat kernels, is one of the most important quality characteristics of oats. Hyperspectral images of oat and groat samples have been acquired by using a NIR spectral camera (Specim, Finland) and the resulting data hypercubes were analyzed applying Principal Component Analysis (PCA) for exploratory purposes and Partial Least Squares-Discriminant Analysis (PLS-DA) to build the classification models to discriminate the two kernel typologies. Results showed that it is possible to accurately recognize oat and groat single kernels by HSI (prediction accuracy was almost 100%). The study demonstrated also that good classification results could be obtained using only three wavelengths (1132, 1195 and 1608 nm), selected by means of a bootstrap-VIP procedure, allowing to speed up the classification processing for industrial applications. The developed objective and non-destructive method based on HSI can be utilized for quality control purposes and/or for the definition of innovative sorting logics of oat grains. PMID:23200388

  11. Hyperspectral image segmentation of the common bile duct

    NASA Astrophysics Data System (ADS)

    Samarov, Daniel; Wehner, Eleanor; Schwarz, Roderich; Zuzak, Karel; Livingston, Edward

    2013-03-01

    Over the course of the last several years hyperspectral imaging (HSI) has seen increased usage in biomedicine. Within the medical field in particular HSI has been recognized as having the potential to make an immediate impact by reducing the risks and complications associated with laparotomies (surgical procedures involving large incisions into the abdominal wall) and related procedures. There are several ongoing studies focused on such applications. Hyperspectral images were acquired during pancreatoduodenectomies (commonly referred to as Whipple procedures), a surgical procedure done to remove cancerous tumors involving the pancreas and gallbladder. As a result of the complexity of the local anatomy, identifying where the common bile duct (CBD) is can be difficult, resulting in comparatively high incidents of injury to the CBD and associated complications. It is here that HSI has the potential to help reduce the risk of such events from happening. Because the bile contained within the CBD exhibits a unique spectral signature, we are able to utilize HSI segmentation algorithms to help in identifying where the CBD is. In the work presented here we discuss approaches to this segmentation problem and present the results.

  12. Non-uniform system response detection for hyperspectral imaging systems

    NASA Astrophysics Data System (ADS)

    Castorena, Juan; Morrison, Jason; Paliwal, Jitendra; Erkinbaev, Chyngyz

    2015-11-01

    Near infrared (NIR) hyperspectral imaging (HSI) has established itself as a powerful non-destructive tool for the chemical analysis of heterogeneous samples. However, one of the main disadvantages of NIR HSI is that the technique suffers from instrumentation-related problems, which in turn affect the acquired images. In general, focal plane array (FPA) based hyperspectral systems are affected by spatial and spectral non-uniform response, the presence of defective sensors (e.g. dead or saturated sensors), and temporal and spatial (e.g. dark current) noise. Another issue is each new camera system needs to be calibrated to assess its specific responses to light. To correct for these issues, we used known standards to measure the response of the sensors and capture the location of the field of view and defective sensors using linear and quadratic models. The parameters of these models were then used as input features for classification of sensor responses using a k-means algorithm. The results conclude that linear models are insufficiently precise for calibration but estimate sufficiently accurately the system's response and functionality. Specifically, it was shown that the classification method discriminates non-responsive regions effectively.

  13. Monitoring biofilm attachment on medical devices surfaces using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Le, Hanh N. D.; Hitchins, Victoria M.; Ilev, Ilko K.; Kim, Do-Hyun

    2014-02-01

    Microbial biofilm is a colony of single bacteria cells (planktonic) that attached to surfaces, attract other microorganisms to attach and grow, and together they build an extracellular matrix composed of polysaccharides, protein, and DNA. Eventually, some cells will detach and spread to other surface. Biofilm on medical devices can cause severe infection to all age ranges from infant to adult. Therefore, it is important to detect biofilm in a fast and efficient manner. Hyperspectral imaging was utilized for distinguishing wide area of biofilm coverage on various materials and on different textures of stainless steeltest coupons. Not only is the coverage of biofilm important, but also the shear stress of biofilm on the attached surfaces is significant. This study investigates the effects of shear stress on the adhesion of biofilms on common medical device surfaces such as glass, polycarbonate, polytetrafluoroethylene, and stainless steel with different textures. Biofilm was grown using Ps. aeruginosa and growth was monitored after 24 and 48 hours at 37° C. The coupons covered with biofilm were tilted at 45 degrees and 90 degrees for 30 seconds to induce shear stress and Hyperspectral images were taken. We hypothesize that stronger attachment on rough surface would be able to withstand greater shear stress compared to smooth surface.

  14. High-performance hyperspectral imaging using virtual slit optics

    NASA Astrophysics Data System (ADS)

    Behr, Bradford B.; Meade, Jeffrey T.; Hajian, Arsen R.; Cenko, Andrew T.

    2013-05-01

    The High Throughput Virtual Slit (or HTVS) is a new optical technology which can significantly increase the throughput and resolution of a dispersive spectrometer. The HTVS is able to preserve spectrometer étendue, mitigating photon losses normally associated with a slit. Originally implemented in multimode fiber-input spectrometers, HTVS has now been shown to be broadly applicable to a wide variety of spatially scanning hyperspectral imagers and standoff sensors, enhancing their performance and unlocking new application areas. In essence, the anamorphic elements of the HTVS optical system provide a means to decouple the spatial (iFOV) and spectral resolution of nearly any HSI system. In some scenarios, HTVS can be used to achieve better spectral resolution with the same input slit width. Alternatively, the slit can be widened (to increase the collected signal) while maintaining the same spectral resolution. This newfound flexibility in optimizing critical performance parameters not only improves the performance of HSI systems in existing remote sensing contexts, but also opens up numerous new application areas which were previously inaccessible to hyperspectral techniques. This method adds substantial value to existing HSI designs, particularly in applications involving targets with large spatial extent and requiring high spectral resolution (e.g. standoff Raman spectroscopy). We present recent experimental results from our prototype HTVS pushbroom imager and discuss case studies of standoff Raman detection of hazardous materials, passive detection of faint narrowband and monochromatic sources, and optimal disentangling of target spectral signatures from the solar spectrum under daytime illumination.

  15. New features for detecting cervical precancer using hyperspectral diagnostic imaging

    NASA Astrophysics Data System (ADS)

    Okimoto, Gordon S.; Parker, Mary F.; Mooradian, Gregory C.; Saggese, Steven J.; Grisanti, Ames A.; O'Connor, Dennis M.; Miyazawa, Kunio

    2001-05-01

    Principal component analysis (PCA) in the wavelet domain provides powerful new features for the non-invasive detection of cervical intraepithelial neoplasia (CIN) using fluorescence imaging spectroscopy. These features are known as principal wavelet components (PWCs). The multiscale structure of the fluorescence spectrum for each pixel of the hyperspectral data cube is extracted using the continuous wavelet transform. PCA is then used to compress and denoise the wavelet representation for presentation to a feed- forward neural network for tissue classification. Using PWC features as inputs to a 5-class NN resulted in average correct classification rates of 95% over five cervical tissue classes corresponding to low-grade dysplasia, squamous, columnar, metaplasia plus a fifth class for other unspecified tissue types, blood and mucus. A 2-class NN was also trained to discriminate between CIN1 and normal tissue with sensitivity and specificity of 98% and 99%, respectively. All performance assessments were based on test data from a set of patients not seen during NN training. Trained neural classifiers were used to `compress' and transform 3D hyperspectral data cubes into 2D color-coded images that accurately mapped the spatial distribution of both normal and dysplastic tissue over the surface of the entire cervix.

  16. Airborne midwave and longwave infrared hyperspectral imaging of gases

    NASA Astrophysics Data System (ADS)

    Gagnon, Marc-André; Tremblay, Pierre; Savary, Simon; Duval, Marc; Farley, Vincent; Chamberland, Martin

    2014-05-01

    Characterization of gas clouds are challenging situations to address due to the large and uneven distribution of these fast moving entities. Whether gas characterization is carried out for gas leaks surveys or environmental monitoring purposes, explosives and/or toxic chemicals are often involved. In such situations, airborne measurements present distinct advantages over ground based-techniques since large areas can be covered efficiently from a safe distance. In order to illustrate the potential of airborne thermal infrared hyperspectral imaging for gas cloud characterization, measurements were carried out above smokestacks and a ground-based gas release experiment. Quantitative airborne chemical images of carbon monoxide (CO) and ethylene (C2H4) were obtained from measurements carried out using a midwave (MWIR, 3-5 μm) and a longwave (LWIR, 8-12 μm) airborne infrared hyperspectral sensor respectively. Scattering effects were observed in the MWIR experiments on smokestacks as a result of water condensation upon rapid cool down of the hot emission gases. Airborne measurements were carried out using both mapping and targeting acquisition modes. The later provides unique time-dependent information such as the gas cloud direction and velocity.

  17. Airborne midwave and longwave infrared hyperspectral imaging of gases

    NASA Astrophysics Data System (ADS)

    Gagnon, Marc-André; Tremblay, Pierre; Savary, Simon; Duval, Marc; Farley, Vincent; Chamberland, Martin

    2014-11-01

    Characterization of gas clouds are challenging situations to address due to the large and uneven distribution of these fast moving entities. Whether gas characterization is carried out for gas leaks surveys or environmental monitoring purposes, explosives and/or toxic chemicals are often involved. In such situations, airborne measurements present distinct advantages over ground based-techniques since large areas can be covered efficiently from a safe distance. In order to illustrate the potential of airborne thermal infrared hyperspectral imaging for gas cloud characterization, measurements were carried out above smokestacks and a ground-based gas release experiment. Quantitative airborne chemical images of carbon monoxide (CO) and ethylene (C2H4) were obtained from measurements carried out using a midwave (MWIR, 3-5 μm) and a longwave (LWIR, 8-12 μm) airborne infrared hyperspectral sensor respectively. Scattering effects were observed in the MWIR experiments on smokestacks as a result of water condensation upon rapid cool down of the hot emission gases. Airborne measurements were carried out using both mapping and targeting acquisition modes. The later provides unique time-dependent information such as the gas cloud direction and velocity.

  18. Airborne midwave and longwave infrared hyperspectral imaging of gases

    NASA Astrophysics Data System (ADS)

    Gagnon, Marc-André; Tremblay, Pierre; Savary, Simon; Duval, Marc; Farley, Vincent; Guyot, Éric; Chamberland, Martin

    2014-10-01

    Characterization of gas clouds are challenging situations to address due to the large and uneven distribution of these fast moving entities. Whether gas characterization is carried out for gas leaks surveys or environmental monitoring purposes, explosives and/or toxic chemicals are often involved. In such situations, airborne measurements present distinct advantages over ground based-techniques since large areas can be covered efficiently from a safe distance. In order to illustrate the potential of airborne thermal infrared hyperspectral imaging for gas cloud characterization, measurements were carried out above smokestacks and a ground-based gas release experiment. Quantitative airborne chemical images of carbon monoxide (CO) and ethylene (C2H4) were obtained from measurements carried out using a midwave (MWIR, 3-5 μm) and a longwave (LWIR, 8-12 μm) airborne infrared hyperspectral sensor respectively. Scattering effects were observed in the MWIR experiments on smokestacks as a result of water condensation upon rapid cool down of the hot emission gases. Airborne measurements were carried out using both mapping and targeting acquisition modes. The later provides unique time-dependent information such as the gas cloud direction and velocity.

  19. HYPERSPECTRAL REFLECTANCE AND FLUORESCENCE LINE-SCAN IMAGING FOR ONLINE QUALITY AND SAFETY INSPECTION OF APPLES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Instrumentation and Sensing Laboratory has recently developed a rapid online line-scan imaging system capable of both hyperspectral Vis/NIR reflectance and fluorescence in the Vis with UV-A excitation. The hyperspectral online line-scan system integrated with a commercial apple-sorting machine ...

  20. Development of a Hyperspectral Imaging System for Online Quality Inspection of Pickling Cucumbers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper reports on the development of a hyperspectral imaging prototype for evaluation of external and internal quality of pickling cucumbers. The prototype consisted of a two-lane round belt conveyor, two illumination sources (one for reflectance and one for transmittance), and a hyperspectral i...

  1. ASTRAL, a hyperspectral imaging DNA sequencer

    NASA Astrophysics Data System (ADS)

    O'Brien, Kevin M.; Wren, Jonathan; Davé, Varshal K.; Bai, Diane; Anderson, Richard D.; Rayner, Simon; Evans, Glen A.; Dabiri, Ali E.; Garner, Harold R.

    1998-05-01

    We are developing a prototype automatic DNA sequencer which utilizes polyacrylamide slab gels imaged through a novel optical detection system. The design of this prototype sequencer allows the ability to perform direct optical coupling over the entire read area of the gel and hyperspectrographic separation and detection of the fluorescence emission. The machine has no moving parts. All the major components incorporated in this prototype are all currently available "off the shelf," thus reducing equipment development time and decreasing costs. Software developed for data acquisition, analysis, and conversion to other standard formats facilitates compatibility.

  2. Identification of early cancerous lesion of esophagus with endoscopic images by hyperspectral image technique (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Huang, Shih-Wei; Chen, Shih-Hua; Chen, Weichung; Wu, I.-Chen; Wu, Ming Tsang; Kuo, Chie-Tong; Wang, Hsiang-Chen

    2016-03-01

    This study presents a method to identify early esophageal cancer within endoscope using hyperspectral imaging technology. The research samples are three kinds of endoscopic images including white light endoscopic, chromoendoscopic, and narrow-band endoscopic images with different stages of pathological changes (normal, dysplasia, dysplasia - esophageal cancer, and esophageal cancer). Research is divided into two parts: first, we analysis the reflectance spectra of endoscopic images with different stages to know the spectral responses by pathological changes. Second, we identified early cancerous lesion of esophagus by principal component analysis (PCA) of the reflectance spectra of endoscopic images. The results of this study show that the identification of early cancerous lesion is possible achieve from three kinds of images. In which the spectral characteristics of NBI endoscopy images of a gray area than those without the existence of the problem the first two, and the trend is very clear. Therefore, if simply to reflect differences in the degree of spectral identification, chromoendoscopic images are suitable samples. The best identification of early esophageal cancer is using the NBI endoscopic images. Based on the results, the use of hyperspectral imaging technology in the early endoscopic esophageal cancer lesion image recognition helps clinicians quickly diagnose. We hope for the future to have a relatively large amount of endoscopic image by establishing a hyperspectral imaging database system developed in this study, so the clinician can take this repository more efficiently preliminary diagnosis.

  3. Improving 3D Wavelet-Based Compression of Hyperspectral Images

    NASA Technical Reports Server (NTRS)

    Klimesh, Matthew; Kiely, Aaron; Xie, Hua; Aranki, Nazeeh

    2009-01-01

    Two methods of increasing the effectiveness of three-dimensional (3D) wavelet-based compression of hyperspectral images have been developed. (As used here, images signifies both images and digital data representing images.) The methods are oriented toward reducing or eliminating detrimental effects of a phenomenon, referred to as spectral ringing, that is described below. In 3D wavelet-based compression, an image is represented by a multiresolution wavelet decomposition consisting of several subbands obtained by applying wavelet transforms in the two spatial dimensions corresponding to the two spatial coordinate axes of the image plane, and by applying wavelet transforms in the spectral dimension. Spectral ringing is named after the more familiar spatial ringing (spurious spatial oscillations) that can be seen parallel to and near edges in ordinary images reconstructed from compressed data. These ringing phenomena are attributable to effects of quantization. In hyperspectral data, the individual spectral bands play the role of edges, causing spurious oscillations to occur in the spectral dimension. In the absence of such corrective measures as the present two methods, spectral ringing can manifest itself as systematic biases in some reconstructed spectral bands and can reduce the effectiveness of compression of spatially-low-pass subbands. One of the two methods is denoted mean subtraction. The basic idea of this method is to subtract mean values from spatial planes of spatially low-pass subbands prior to encoding, because (a) such spatial planes often have mean values that are far from zero and (b) zero-mean data are better suited for compression by methods that are effective for subbands of two-dimensional (2D) images. In this method, after the 3D wavelet decomposition is performed, mean values are computed for and subtracted from each spatial plane of each spatially-low-pass subband. The resulting data are converted to sign-magnitude form and compressed in a

  4. Hyperspectral and multispectral bioluminescence optical tomography for small animal imaging.

    PubMed

    Chaudhari, Abhijit J; Darvas, Felix; Bading, James R; Moats, Rex A; Conti, Peter S; Smith, Desmond J; Cherry, Simon R; Leahy, Richard M

    2005-12-01

    For bioluminescence imaging studies in small animals, it is important to be able to accurately localize the three-dimensional (3D) distribution of the underlying bioluminescent source. The spectrum of light produced by the source that escapes the subject varies with the depth of the emission source because of the wavelength-dependence of the optical properties of tissue. Consequently, multispectral or hyperspectral data acquisition should help in the 3D localization of deep sources. In this paper, we describe a framework for fully 3D bioluminescence tomographic image acquisition and reconstruction that exploits spectral information. We describe regularized tomographic reconstruction techniques that use semi-infinite slab or FEM-based diffusion approximations of photon transport through turbid media. Singular value decomposition analysis was used for data dimensionality reduction and to illustrate the advantage of using hyperspectral rather than achromatic data. Simulation studies in an atlas-mouse geometry indicated that sub-millimeter resolution may be attainable given accurate knowledge of the optical properties of the animal. A fixed arrangement of mirrors and a single CCD camera were used for simultaneous acquisition of multispectral imaging data over most of the surface of the animal. Phantom studies conducted using this system demonstrated our ability to accurately localize deep point-like sources and show that a resolution of 1.5 to 2.2 mm for depths up to 6 mm can be achieved. We also include an in vivo study of a mouse with a brain tumour expressing firefly luciferase. Co-registration of the reconstructed 3D bioluminescent image with magnetic resonance images indicated good anatomical localization of the tumour. PMID:16306643

  5. Hyperspectral and multispectral bioluminescence optical tomography for small animal imaging

    NASA Astrophysics Data System (ADS)

    Chaudhari, Abhijit J.; Darvas, Felix; Bading, James R.; Moats, Rex A.; Conti, Peter S.; Smith, Desmond J.; Cherry, Simon R.; Leahy, Richard M.

    2005-12-01

    For bioluminescence imaging studies in small animals, it is important to be able to accurately localize the three-dimensional (3D) distribution of the underlying bioluminescent source. The spectrum of light produced by the source that escapes the subject varies with the depth of the emission source because of the wavelength-dependence of the optical properties of tissue. Consequently, multispectral or hyperspectral data acquisition should help in the 3D localization of deep sources. In this paper, we describe a framework for fully 3D bioluminescence tomographic image acquisition and reconstruction that exploits spectral information. We describe regularized tomographic reconstruction techniques that use semi-infinite slab or FEM-based diffusion approximations of photon transport through turbid media. Singular value decomposition analysis was used for data dimensionality reduction and to illustrate the advantage of using hyperspectral rather than achromatic data. Simulation studies in an atlas-mouse geometry indicated that sub-millimeter resolution may be attainable given accurate knowledge of the optical properties of the animal. A fixed arrangement of mirrors and a single CCD camera were used for simultaneous acquisition of multispectral imaging data over most of the surface of the animal. Phantom studies conducted using this system demonstrated our ability to accurately localize deep point-like sources and show that a resolution of 1.5 to 2.2 mm for depths up to 6 mm can be achieved. We also include an in vivo study of a mouse with a brain tumour expressing firefly luciferase. Co-registration of the reconstructed 3D bioluminescent image with magnetic resonance images indicated good anatomical localization of the tumour.

  6. Pattern recognition via multispectral, hyperspectral, and polarization-based imaging

    NASA Astrophysics Data System (ADS)

    El-Saba, Aed; Alam, Mohammad S.; Sakla, Wesam A.

    2010-04-01

    Pattern recognition deals with the detection and identification of a specific target in an unknown input scene. Target features such as shape, color, surface dynamics, and material characteristics are common target attributes used for identification and detection purposes. Pattern recognition using multispectral (MS), hyperspectral (HS), and polarization-based spectral (PS) imaging can be effectively exploited to highlight one or more of these attributes for more efficient target identification and detection. In general, pattern recognition involves two steps: gathering target information from sensor data and identifying and detecting the desired target from sensor data in the presence of noise, clutter, and other artifacts. Multispectral and hyperspectral imaging (MSI/HSI) provide both spectral and spatial information about the target. As the reflection or emission spectral signatures depend on the elemental composition of objects residing within the scene, the polarization state of radiation is sensitive to the surface features such as relative smoothness or roughness, surface material, shapes and edges, etc. Therefore, polarization information imparted by surface reflections of the target yields unique and discriminatory signatures which could be used to augment spectral target detection techniques, through the fusion of sensor data. Sensor data fusion is currently being used to effectively recognize and detect one or more of the target attributes. However, variations between sensors and temporal changes within sensors can introduce noise in the measurements, contributing to additional target variability that hinders the detection process. This paper provides a quick overview of target identification and detection using MSI/HSI, highlighting the advantages and disadvantages of each. It then discusses the effectiveness of using polarization-based imaging in highlighting some of the target attributes at single and multiple spectral bands using polarization

  7. [Detection of Hawthorn Fruit Defects Using Hyperspectral Imaging].

    PubMed

    Liu, De-hua; Zhang, Shu-juan; Wang, Bin; Yu, Ke-qiang; Zhao, Yan-ru; He, Yong

    2015-11-01

    Hyperspectral imaging technology covered the range of 380-1000 nm was employed to detect defects (bruise and insect damage) of hawthorn fruit. A total of 134 samples were collected, which included damage fruit of 46, pest fruit of 30, injure and pest fruit of 10 and intact fruit of 48. Because calyx · s⁻¹ tem-end and bruise/insect damage regions offered a similar appearance characteristic in RGB images, which could produce easily confusion between them. Hence, five types of defects including bruise, insect damage, sound, calyx, and stem-end were collected from 230 hawthorn fruits. After acquiring hyperspectral images of hawthorn fruits, the spectral data were extracted from region of interest (ROI). Then, several pretreatment methods of standard normalized variate (SNV), savitzky golay (SG), median filter (MF) and multiplicative scatter correction (MSC) were used and partial least squares method(PLS) model was carried out to obtain the better performance. Accordingly to their results, SNV pretreatment methods assessed by PLS was viewed as best pretreatment method. Lastly, SNV was chosen as the pretreatment method. Spectral features of five different regions were combined with Regression coefficients(RCs) of partial least squares-discriminant analysis (PLS-DA) model was used to identify the important wavelengths and ten wavebands at 483, 563, 645, 671, 686, 722, 777, 819, 837 and 942 nm were selected from all of the wavebands. Using Kennard-Stone algorithm, all kinds of samples were randomly divided into training set (173) and test set (57) according to the proportion of 3:1. And then, least squares-support vector machine (LS-SVM) discriminate model was established by using the selected wavebands. The results showed that the discriminate accuracy of the method was 91.23%. In the other hand, images at ten important wavebands were executed to Principal component analysis (PCA). Using "Sobel" operator and region growing algrorithm "Regiongrow", the edge and defect

  8. Near-infrared hyperspectral imaging for quality analysis of agricultural and food products

    NASA Astrophysics Data System (ADS)

    Singh, C. B.; Jayas, D. S.; Paliwal, J.; White, N. D. G.

    2010-04-01

    Agricultural and food processing industries are always looking to implement real-time quality monitoring techniques as a part of good manufacturing practices (GMPs) to ensure high-quality and safety of their products. Near-infrared (NIR) hyperspectral imaging is gaining popularity as a powerful non-destructive tool for quality analysis of several agricultural and food products. This technique has the ability to analyse spectral data in a spatially resolved manner (i.e., each pixel in the image has its own spectrum) by applying both conventional image processing and chemometric tools used in spectral analyses. Hyperspectral imaging technique has demonstrated potential in detecting defects and contaminants in meats, fruits, cereals, and processed food products. This paper discusses the methodology of hyperspectral imaging in terms of hardware, software, calibration, data acquisition and compression, and development of prediction and classification algorithms and it presents a thorough review of the current applications of hyperspectral imaging in the analyses of agricultural and food products.

  9. Learning Hierarchical Spectral-Spatial Features for Hyperspectral Image Classification.

    PubMed

    Zhou, Yicong; Wei, Yantao

    2016-07-01

    This paper proposes a spectral-spatial feature learning (SSFL) method to obtain robust features of hyperspectral images (HSIs). It combines the spectral feature learning and spatial feature learning in a hierarchical fashion. Stacking a set of SSFL units, a deep hierarchical model called the spectral-spatial networks (SSN) is further proposed for HSI classification. SSN can exploit both discriminative spectral and spatial information simultaneously. Specifically, SSN learns useful high-level features by alternating between spectral and spatial feature learning operations. Then, kernel-based extreme learning machine (KELM), a shallow neural network, is embedded in SSN to classify image pixels. Extensive experiments are performed on two benchmark HSI datasets to verify the effectiveness of SSN. Compared with state-of-the-art methods, SSN with a deep hierarchical architecture obtains higher classification accuracy in terms of the overall accuracy, average accuracy, and kappa ( κ ) coefficient of agreement, especially when the number of the training samples is small. PMID:26241988

  10. Application of the MAP estimation model to hyperspectral resolution image enhancement

    NASA Astrophysics Data System (ADS)

    Dong, Guangjun; Zhou, Haifang; Ji, Song; Shu, Rong

    2009-10-01

    This paper makes a study of maximum a posteriori (MAP) estimation method for enhancing the spatial resolution of a hyperspectral image using a higher resolution coincident panchromatic image. Here, the mathematical formulation of the proposed MAP method is described and the detail process step is introduced. Then, enhancement results using PHI hyperspectral image datasets are provided. In general, it is found that the MAP method is able to obtain high-resolution hyperspectral data. Experiment shows that the method is effective while the enhancement for conventional methods, like average estimation, is limited primarily to fuse spectral information.

  11. Detection of hypercholesterolemia using hyperspectral imaging of human skin

    NASA Astrophysics Data System (ADS)

    Milanic, Matija; Bjorgan, Asgeir; Larsson, Marcus; Strömberg, Tomas; Randeberg, Lise L.

    2015-07-01

    Hypercholesterolemia is characterized by high blood levels of cholesterol and is associated with increased risk of atherosclerosis and cardiovascular disease. Xanthelasma is a subcutaneous lesion appearing in the skin around the eyes. Xanthelasma is related to hypercholesterolemia. Identifying micro-xanthelasma can thereforeprovide a mean for early detection of hypercholesterolemia and prevent onset and progress of disease. The goal of this study was to investigate spectral and spatial characteristics of hypercholesterolemia in facial skin. Optical techniques like hyperspectral imaging (HSI) might be a suitable tool for such characterization as it simultaneously provides high resolution spatial and spectral information. In this study a 3D Monte Carlo model of lipid inclusions in human skin was developed to create hyperspectral images in the spectral range 400-1090 nm. Four lesions with diameters 0.12-1.0 mm were simulated for three different skin types. The simulations were analyzed using three algorithms: the Tissue Indices (TI), the two layer Diffusion Approximation (DA), and the Minimum Noise Fraction transform (MNF). The simulated lesions were detected by all methods, but the best performance was obtained by the MNF algorithm. The results were verified using data from 11 volunteers with known cholesterol levels. The face of the volunteers was imaged by a LCTF system (400- 720 nm), and the images were analyzed using the previously mentioned algorithms. The identified features were then compared to the known cholesterol levels of the subjects. Significant correlation was obtained for the MNF algorithm only. This study demonstrates that HSI can be a promising, rapid modality for detection of hypercholesterolemia.

  12. Spectral homogenization techniques for the hyperspectral image projector

    NASA Astrophysics Data System (ADS)

    Hillberry, Logan E.; Rice, Joseph P.

    2015-05-01

    In an effort to improve technology for performance testing and calibration of multispectral and hyperspectral imagers, the National Institute of Standards and Technology (NIST) has been developing a Hyperspectral Image Projector (HIP) capable of projecting dynamic scenes than include distinct, programmable spectra in each of its 1024x768 spatial pixels. The HIP is comprised of a spectral engine, which is a light source capable generating the spectra in the scene, coupled to a spatial engine, capable of projecting the spectra into the correct locations of the scene. In the prototype HIP, the light exiting the Visible-Near-Infrared (VNIR) / Short-Wavelength Infrared (SWIR) spectral engine is spectrally dispersed and needs to be spectrally homogenized before it enters the spatial engine. In this paper we describe the results from a study of several different techniques for performing this spectral homogenization. These techniques include an integrating sphere, a liquid light guide, a randomized fiber bundle, and an engineered diffuser, in various combinations. The spectral uniformity of projected HIP scenes is measured and analyzed using the spectral angle mapper (SAM) algorithm over the VNIR spectral range. The SAM provides a way to analyze the spectral uniformity independently from the radiometric uniformity. The goal of the homogenizer is a spectrally uniform and bright projected image. An integrating sphere provides the most spectrally uniform image, but at a great loss of light compared with the other methods. The randomized fiber bundle generally outperforms the liquid light guide in both spectral homogenization and brightness. Using an engineered diffuser with the randomized fiber bundle increases the spectral uniformity by a factor of five, with a decrease in brightness by a factor of five, compared with the randomized fiber bundle alone. The combination of an engineered diffuser with a randomized fiber bundle provides comparable spectral uniformity to the

  13. Framelet-Based Sparse Unmixing of Hyperspectral Images.

    PubMed

    Zhang, Guixu; Xu, Yingying; Fang, Faming

    2016-04-01

    Spectral unmixing aims at estimating the proportions (abundances) of pure spectrums (endmembers) in each mixed pixel of hyperspectral data. Recently, a semi-supervised approach, which takes the spectral library as prior knowledge, has been attracting much attention in unmixing. In this paper, we propose a new semi-supervised unmixing model, termed framelet-based sparse unmixing (FSU), which promotes the abundance sparsity in framelet domain and discriminates the approximation and detail components of hyperspectral data after framelet decomposition. Due to the advantages of the framelet representations, e.g., images have good sparse approximations in framelet domain, and most of the additive noises are included in the detail coefficients, the FSU model has a better antinoise capability, and accordingly leads to more desirable unmixing performance. The existence and uniqueness of the minimizer of the FSU model are then discussed, and the split Bregman algorithm and its convergence property are presented to obtain the minimal solution. Experimental results on both simulated data and real data demonstrate that the FSU model generally performs better than the compared methods. PMID:26849863

  14. Detecting liquid contamination on surfaces using hyperspectral imaging data

    NASA Astrophysics Data System (ADS)

    Warren, Russell E.; Cohn, David B.; Gagnon, Marc-André; Farley, Vincent

    2015-05-01

    Over the past two years we have developed a new approach for detecting and identifying the presence of liquid chemical contamination on surfaces using hyperspectral imaging data. This work requires an algorithm for unmixing the data to separate the liquid contamination component of the data from all other possible spectral effects, such as the illumination and reflectance spectra of the pure background. The contamination components from S and P polarized reflectance data are then used to estimate the complex refractive index. We retain the index estimates within spectral windows chosen for each of a set of candidate contaminant materials based on their optical extinction. Spectral estimates within those windows are characteristic of the liquid material, and can be passed on to an algorithm for chemical detection and identification. The resulting algorithm is insensitive to the composition of the surface material, and requires no prior measurements of the uncontaminated surface. In a series of field tests, data from the Telops Hyper-Cam sensor were used to develop and validate our approach. We discuss our hyperspectral unmixing and index estimation approaches, and show results from tests conducted at the Telops facility in Québec under a contract with the U.S. Army Edgewood Chemical Biological Center.

  15. Maximum margin metric learning based target detection for hyperspectral images

    NASA Astrophysics Data System (ADS)

    Dong, Yanni; Zhang, Liangpei; Zhang, Lefei; Du, Bo

    2015-10-01

    Target detection is one of the most important problems in hyperspectral image (HSI) processing. However, the classical algorithms depend on the specific statistical hypothesis test, and the algorithms may only perform well under certain conditions, e.g., the adaptive matched subspace detector algorithm assumes that the background covariance matrices do not include the target signatures, which seldom happens in the real world. How to develop a proper metric for measuring the separability between targets and backgrounds becomes the key in target detection. This paper proposes an efficient maximum margin metric learning (MMML) based target detection algorithm, which aims at exploring the limited samples in metric learning and transfers the metric learning problem for hyperspectral target detection into a maximum margin problem which can be optimized via a cutting plane method, and maximally separates the target samples from the background ones. The extensive experimental results with different HSIs demonstrate that the proposed method outperforms both the state-of-the-art target detection algorithms and the other classical metric learning methods.

  16. Towards a colony counting system using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Masschelein, B.; Robles-Kelly, A.; Blanch, C.; Tack, N.; Simpson-Young, B.; Lambrechts, A.

    2012-03-01

    Colony counting is a procedure used in microbiology laboratories for food quality monitoring, environmental management, etc. Its purpose is to detect the level of contamination due to the presence and growth of bacteria, yeasts and molds in a given product. Current automated counters require a tedious training and setup procedure per product and bacteria type and do not cope well with diversity. This contrasts with the setting at microbiology laboratories, where a wide variety of food and bacteria types have to be screened on a daily basis. To overcome the limitations of current systems, we propose the use of hyperspectral imaging technology and examine the spectral variations induced by factors such as illumination, bacteria type, food source and age and type of the agar. To this end, we perform experiments making use of two alternative hyperspectral processing pipelines and compare our classification results to those yielded by color imagery. Our results show that colony counting may be automated through the automatic recovery of the illuminant power spectrum and reflectance. This is consistent with the notion that the recovery of the illuminant should minimize the variations in the spectra due to reflections, shadows and other photometric artifacts. We also illustrate how, with the reflectance at hand, the colonies can be counted making use of classical segmentation and classification algorithms.

  17. Excitation-scanning hyperspectral imaging system for microscopic and endoscopic applications

    NASA Astrophysics Data System (ADS)

    Mayes, Sam A.; Leavesley, Silas J.; Rich, Thomas C.

    2016-04-01

    Current microscopic and endoscopic technologies for cancer screening utilize white-light illumination sources. Hyper-spectral imaging has been shown to improve sensitivity while retaining specificity when compared to white-light imaging in both microscopy and in vivo imaging. However, hyperspectral imaging methods have historically suffered from slow acquisition times due to the narrow bandwidth of spectral filters. Often minutes are required to gather a full image stack. We have developed a novel approach called excitation-scanning hyperspectral imaging that provides 2-3 orders of magnitude increased signal strength. This reduces acquisition times significantly, allowing for live video acquisition. Here, we describe a preliminary prototype excitation-scanning hyperspectral imaging system that can be coupled with endoscopes or microscopes for hyperspectral imaging of tissues and cells. Our system is comprised of three subsystems: illumination, transmission, and imaging. The illumination subsystem employs light-emitting diode arrays to illuminate at different wavelengths. The transmission subsystem utilizes a unique geometry of optics and a liquid light guide. Software controls allow us to interface with and control the subsystems and components. Digital and analog signals are used to coordinate wavelength intensity, cycling and camera triggering. Testing of the system shows it can cycle 16 wavelengths at as fast as 1 ms per cycle. Additionally, more than 18% of the light transmits through the system. Our setup should allow for hyperspectral imaging of tissue and cells in real time.

  18. Miniaturization of sub-meter resolution hyperspectral imagers on unmanned aerial systems

    NASA Astrophysics Data System (ADS)

    Hill, Samuel L.; Clemens, Peter

    2014-05-01

    Traditional airborne environmental monitoring has frequently deployed hyperspectral imaging as a leading tool for characterizing and analyzing a scene's critical spectrum-based signatures for applications in agriculture genomics and crop health, vegetation and mineral monitoring, and hazardous material detection. As the acceptance of hyperspectral evaluation grows in the airborne community, there has been a dramatic trend in moving the technology from use on midsize aircraft to Unmanned Aerial Systems (UAS). The use of UAS accomplishes a number of goals including the reduction in cost to run multiple seasonal evaluations over smaller but highly valuable land-areas, the ability to use frequent data collections to make rapid decisions on land management, and the improvement of spatial resolution by flying at lower altitudes (< 150 m). Despite this trend, there are several key parameters affecting the use of traditional hyperspectral instruments in UAS with payloads less than 0.5 kg (~1lb) where size, weight and power (SWaP) are critical to how high and how far a given UAS can fly. Additionally, on many of the light-weight UAS, users are frequently trying to capture data from one or more instruments to augment the hyperspectral data collection, thus reducing the amount of SWaP available to the hyperspectral instrumentation. The following manuscript will provide an analysis on a newly-developed miniaturized hyperspectral imaging platform that provides full hyperspectral resolution and traditional hyperspectral capabilities without sacrificing performance to accommodate the decreasing SWaP of smaller and smaller UAS platforms.

  19. Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets.

    PubMed

    Lavagnino, Zeno; Dwight, Jason; Ustione, Alessandro; Nguyen, Thuc-Uyen; Tkaczyk, Tomasz S; Piston, David W

    2016-07-26

    The observation of ionic signaling dynamics in intact pancreatic islets has contributed greatly to our understanding of both α- and β-cell function. Insulin secretion from β-cells depends on the firing of action potentials and consequent rises of intracellular calcium activity ([Ca(2+)]i). Zinc (Zn(2+)) is cosecreted with insulin, and has been postulated to play a role in cell-to-cell cross talk within an islet, in particular inhibiting glucagon secretion from α-cells. Thus, measuring [Ca(2+)]i and Zn(2+) dynamics from both α- and β-cells will elucidate mechanisms underlying islet hormone secretion. [Ca(2+)]i and intracellular Zn(2+) can be measured using fluorescent biosensors, but the most efficient sensors have overlapping spectra that complicate their discrimination. Hyperspectral imaging can be used to distinguish signals from multiple fluorophores, but available hyperspectral implementations are either too slow to measure the dynamics of ionic signals or not suitable for thick samples. We have developed a five-dimensional (x,y,z,t,λ) imaging system that leverages a snapshot hyperspectral imaging method, image mapping spectrometry, and light-sheet microscopy. This system provides subsecond temporal resolution from deep within multicellular structures. Using a single excitation wavelength (488 nm) we acquired images from triply labeled samples with two biosensors and a genetically expressing fluorescent protein (spectrally overlapping with one of the biosensors) with high temporal resolution. Measurements of [Ca(2+)]i and Zn(2+) within both α- and β-cells as a function of glucose concentration show heterogeneous uptake of Zn(2+) into α-cells that correlates to the known heterogeneities in [Ca(2+)]i. These differences in intracellular Zn(2+) among α-cells may contribute to the inhibition in glucagon secretion observed at elevated glucose levels. PMID:27463142

  20. High speed measurement of corn seed viability using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Ambrose, Ashabahebwa; Kandpal, Lalit Mohan; Kim, Moon S.; Lee, Wang-Hee; Cho, Byoung-Kwan

    2016-03-01

    Corn is one of the most cultivated crops all over world as food for humans as well as animals. Optimized agronomic practices and improved technological interventions during planting, harvesting and post-harvest handling are critical to improving the quantity and quality of corn production. Seed germination and vigor are the primary determinants of high yield notwithstanding any other factors that may play during the growth period. Seed viability may be lost during storage due to unfavorable conditions e.g. moisture content and temperatures, or physical damage during mechanical processing e.g. shelling, or over heating during drying. It is therefore vital for seed companies and farmers to test and ascertain seed viability to avoid losses of any kind. This study aimed at investigating the possibility of using hyperspectral imaging (HSI) technique to discriminate viable and nonviable corn seeds. A group of corn samples were heat treated by using microwave process while a group of seeds were kept as control group (untreated). The hyperspectral images of corn seeds of both groups were captured between 400 and 2500 nm wave range. Partial least squares discriminant analysis (PLS-DA) was built for the classification of aged (heat treated) and normal (untreated) corn seeds. The model showed highest classification accuracy of 97.6% (calibration) and 95.6% (prediction) in the SWIR region of the HSI. Furthermore, the PLS-DA and binary images were capable to provide the visual information of treated and untreated corn seeds. The overall results suggest that HSI technique is accurate for classification of viable and non-viable seeds with non-destructive manner.

  1. Hyper-spectral imaging using an optical fiber transition element

    NASA Astrophysics Data System (ADS)

    Bush, Brett C.; Otten, Leonard J., III; Schmoll, Juergen

    2007-09-01

    The Bi-static Optical Imaging Sensor (BOIS) is a 2-D imaging sensor that operates in the short-wave infra-red (SWIR) spectral regime over wavelengths from approximately 1.0 to 2.4 microns. The conceptual design of the sensor is based on integral field spectroscopy techniques. The BOIS sensor utilizes a fiber transition element consisting of multiple optical fibers to map the 2-D spatial input scene into a 1-D linear array for injection into a hyper-spectral imaging (HSI) sensor. The HSI spectrometer acquires fast time resolution snapshots (60 Hz) of the entire input target scene in numerous narrowband spectral channels covering the SWIR spectral band. The BOIS sensor is developed to spatially observe the fast time-evolving radiative signature of targets over a variety of spectral bands, thus simultaneously characterizing the overall scene in four dimensions: 2 spatial, wavelength, and time. We describe the successful design, operation, and testing of a laboratory prototype version of the BOIS sensor as well as further development of a field version of the sensor. The goal of the laboratory prototype BOIS sensor was to validate the proof-of-concept ability in the 4-D measurement concept of this unique design. We demonstrate the 2-D spatial remapping of the input scene (using SWIR laser and blackbody cavity sources) in multiple spectral channels from the spatial versus spectral pixel output of the HSI snapshot. We also describe algorithms developed in the data processing to retrieve temperatures of the observation scene from the hyper-spectral measurements.

  2. Multi- and hyperspectral UAV imaging system for forest and agriculture applications

    NASA Astrophysics Data System (ADS)

    Mäkynen, Jussi; Saari, Heikki; Holmlund, Christer; Mannila, Rami; Antila, Tapani

    2012-06-01

    VTT Technical Research Centre of Finland has developed a Fabry-Perot Interferometer (FPI) based hyperspectral imager compatible with light weight UAV (Unmanned Aerial Vehicle) platforms (SPIE Proc. 74741, 8186B2). The FPI based hyperspectral imager was used in a UAV imaging campaign for forest and agriculture tests during the summer 2011 (SPIE Proc. 81743). During these tests high spatial resolution Color-Infrared (CIR) images and hyperspectral images were recorded on separate flights. The spectral bands of the CIR camera were 500 - 580 nm for the green band, 580 - 700 nm for the red band and 700 - 1000 nm for the near infrared band. For the summer 2012 flight campaign a new hyperspectral imager is currently being developed. A custom made CIR camera will also be used. The system which includes both the high spatial resolution Color-Infrared camera and a light weight hyperspectral imager can provide all necessary data with just one UAV flight over the target area. The new UAV imaging system contains a 4 Megapixel CIR camera which is used for the generation of the digital surface models and CIR mosaics. The hyperspectral data can be recorded in the wavelength range 500 - 900 nm at a resolution of 10 - 30 nm at FWHM. The resolution can be selected from approximate values of 10, 15, 20 or 30 nm at FWHM.

  3. Hyperspectral imaging for the detection of retinal disease

    NASA Astrophysics Data System (ADS)

    Harvey, Andrew R.; Lawlor, Joanne; McNaught, Andrew I.; Williams, John W.; Fletcher-Holmes, David W.

    2002-11-01

    Hyperspectral imaging (HSI) shows great promise for the detection and classification of several diseases, particularly in the fields of "optical biopsy" as applied to oncology, and functional retinal imaging in ophthalmology. In this paper, we discuss the application of HSI to the detection of retinal diseases and technological solutions that address some of the fundamental difficulties of spectral imaging within the eye. HSI of the retina offers a route to non-invasively deduce biochemical and metabolic processes within the retina. For example it shows promise for the mapping of retinal blood perfusion using spectral signatures of oxygenated and deoxygenated hemoglobin. Compared with other techniques using just a few spectral measurements, it offers improved classification in the presence of spectral cross-contamination by pigments and other components within the retina. There are potential applications for this imaging technique in the investigation and treatment of the eye complications of diabetes, and other diseases involving disturbances to the retinal, or optic-nerve-head circulation. It is well known that high-performance HSI requires high signal-to-noise ratios (SNR) whereas the application of any imaging technique within the eye must cope with the twin limitations of the small numerical aperture provided by the entrance pupil to the eye and the limit on the radiant power at the retina. We advocate the use of spectrally-multiplexed spectral imaging techniques (the traditional filter wheel is a traditional example). These approaches enable a flexible approach to spectral imaging, with wider spectral range, higher SNRs and lower light intensity at the retina than could be achieved using a Fourier-transform (FT) approach. We report the use of spectral imaging to provide calibrated spectral albedo images of healthy and diseased retinas and the use of this data for screening purposes. These images clearly demonstrate the ability to distinguish between

  4. A prior knowledge model for multidimensional striping noise compensation in hyperspectral imaging devices

    NASA Astrophysics Data System (ADS)

    Meza, Pablo; Pezoa, Jorge E.; Parra, Francisca; Torres, Sergio N.

    2012-09-01

    In this paper, a prior knowledge model is proposed in order to increase the effectiveness of a multidimensional striping noise compensation (SNC) algorithm. This is accomplished by considering an optoelectronic approach, thereby generating a more accurate mathematical representation of the hyperspectral acquisition process. The proposed model includes knowledge on the system spectral response, which can be obtained by means of an input with known spectral radiation. Further, the model also considers the dependence of the noise structure on the analog-digital conversion process, that is, schemes such as active-pixel sensor (APS) and passive-pixel sensor (PPS) have been considered. Finally, the model takes advantage of the degree of crosstalk between consecutive bands in order to determinate how much of this spectral information is contributing to the read out data obtained in a particular band. All prior knowledge is obtained by a series of experimental analysis, and then integrated into the model. After estimating the required parameters, the applicability of the multidimensional SNC is illustrated by compensating for stripping noise in hyperspectral images acquired using an experimental setup. A laboratory prototype, based on both a Photonfocus Hurricane hyperspectral camera and a Xeva Xenics NIR hyperspectral camera, has been implemented to acquire data in the range of 400-1000 [nm] and 900-1700 [nm], respectively. Also, a mobile platform has been used to simulate and synchronize the scanning procedure of the cameras and an uniform tungsten lamp has been installed to ensure an equal spectral radiance between the different bands for calibration purpose.

  5. ICER-3D: A Progressive Wavelet-Based Compressor for Hyperspectral Images

    NASA Technical Reports Server (NTRS)

    Kiely, A.; Klimesh, M.; Xie, H.; Aranki, N.

    2005-01-01

    ICER-3D is a progressive, wavelet-based compressor for hyperspectral images. ICER-3D is derived from the ICER image compressor. ICER-3D can provide lossless and lossy compression, and incorporates an error-containment scheme to limit the effects of data loss during transmission. The three-dimensional wavelet decomposition structure used by ICER-3D exploits correlations in all three dimensions of hyperspectral data sets, while facilitating elimination of spectral ringing artifacts. Correlation is further exploited by a context modeler that effectively exploits spectral dependencies in the wavelet-transformed hyperspectral data. Performance results illustrating the benefits of these features are presented.

  6. Infrared adaptive spectral imagers for direct detection of spectral signatures and hyperspectral imagery

    NASA Astrophysics Data System (ADS)

    Goldstein, Neil; Fox, Marsha; Adler-Golden, Steven; Gregor, Brian

    2013-03-01

    Field test results are presented for a prototype long-wave adaptive imager that provides both hyperspectral imagery and contrast imagery based on the direct application of hyperspectral detection algorithms in hardware. Programmable spatial light modulators are used to provide both spectral and spatial resolution using a single element detector. Programmable spectral and spatial detection filters can be used to superimpose any possible analog spectral detection filter on the image. In this work, we demonstrate three modes of operation, including hyperspectral imagery, and one and two-dimensional imagery using a generalized matched filter for detection of a specific target gas within the scene.

  7. Optimizing extreme learning machine for hyperspectral image classification

    NASA Astrophysics Data System (ADS)

    Li, Jiaojiao; Du, Qian; Li, Wei; Li, Yunsong

    2015-01-01

    Extreme learning machine (ELM) is of great interest to the machine learning society due to its extremely simple training step. Its performance sensitivity to the number of hidden neurons is studied under the context of hyperspectral remote sensing image classification. An empirical linear relationship between the number of training samples and the number of hidden neurons is proposed. Such a relationship can be easily estimated with two small training sets and extended to large training sets to greatly reduce computational cost. The kernel version of ELM (KELM) is also implemented with the radial basis function kernel, and such a linear relationship is still suitable. The experimental results demonstrated that when the number of hidden neurons is appropriate, the performance of ELM may be slightly lower than the linear SVM, but the performance of KELM can be comparable to the kernel version of SVM (KSVM). The computational cost of ELM and KELM is much lower than that of the linear SVM and KSVM, respectively.

  8. Kernel weighted joint collaborative representation for hyperspectral image classification

    NASA Astrophysics Data System (ADS)

    Du, Qian; Li, Wei

    2015-05-01

    Collaborative representation classifier (CRC) has been applied to hyperspectral image classification, which intends to use all the atoms in a dictionary to represent a testing pixel for label assignment. However, some atoms that are very dissimilar to the testing pixel should not participate in the representation, or their contribution should be very little. The regularized version of CRC imposes strong penalty to prevent dissimilar atoms with having large representation coefficients. To utilize spatial information, the weighted sum of local spatial neighbors is considered as a joint spatial-spectral feature, which is actually for regularized CRC-based classification. This paper proposes its kernel version to further improve classification accuracy, which can be higher than those from the traditional support vector machine with composite kernel and the kernel version of sparse representation classifier.

  9. Probability Density and CFAR Threshold Estimation for Hyperspectral Imaging

    SciTech Connect

    Clark, G A

    2004-09-21

    The work reported here shows the proof of principle (using a small data set) for a suite of algorithms designed to estimate the probability density function of hyperspectral background data and compute the appropriate Constant False Alarm Rate (CFAR) matched filter decision threshold for a chemical plume detector. Future work will provide a thorough demonstration of the algorithms and their performance with a large data set. The LASI (Large Aperture Search Initiative) Project involves instrumentation and image processing for hyperspectral images of chemical plumes in the atmosphere. The work reported here involves research and development on algorithms for reducing the false alarm rate in chemical plume detection and identification algorithms operating on hyperspectral image cubes. The chemical plume detection algorithms to date have used matched filters designed using generalized maximum likelihood ratio hypothesis testing algorithms [1, 2, 5, 6, 7, 12, 10, 11, 13]. One of the key challenges in hyperspectral imaging research is the high false alarm rate that often results from the plume detector [1, 2]. The overall goal of this work is to extend the classical matched filter detector to apply Constant False Alarm Rate (CFAR) methods to reduce the false alarm rate, or Probability of False Alarm P{sub FA} of the matched filter [4, 8, 9, 12]. A detector designer is interested in minimizing the probability of false alarm while simultaneously maximizing the probability of detection P{sub D}. This is summarized by the Receiver Operating Characteristic Curve (ROC) [10, 11], which is actually a family of curves depicting P{sub D} vs. P{sub FA}parameterized by varying levels of signal to noise (or clutter) ratio (SNR or SCR). Often, it is advantageous to be able to specify a desired P{sub FA} and develop a ROC curve (P{sub D} vs. decision threshold r{sub 0}) for that case. That is the purpose of this work. Specifically, this work develops a set of algorithms and MATLAB

  10. Aliasing removing of hyperspectral image based on fractal structure matching

    NASA Astrophysics Data System (ADS)

    Wei, Ran; Zhang, Ye; Zhang, Junping

    2015-05-01

    Due to the richness on high frequency components, hyperspectral image (HSI) is more sensitive to distortion like aliasing. Many methods aiming at removing such distortion have been proposed. However, seldom of them are suitable to HSI, due to low spatial resolution characteristic of HSI. Fortunately, HSI contains plentiful spectral information, which can be exploited to overcome such difficulties. Motivated by this, we proposed an aliasing removing method for HSI. The major differences between proposed and current methods is that proposed algorithm is able to utilize fractal structure information, thus the dilemma originated from low-resolution of HSI is solved. Experiments on real HSI data demonstrated subjectively and objectively that proposed method can not only remove annoying visual effect brought by aliasing, but also recover more high frequency component.

  11. Full field imaging based instantaneous hyperspectral absolute refractive index measurement

    SciTech Connect

    Baba, Justin S; Boudreaux, Philip R

    2012-01-01

    Multispectral refractometers typically measure refractive index (RI) at discrete monochromatic wavelengths via a serial process. We report on the demonstration of a white light full field imaging based refractometer capable of instantaneous multispectral measurement of absolute RI of clear liquid/gel samples across the entire visible light spectrum. The broad optical bandwidth refractometer is capable of hyperspectral measurement of RI in the range 1.30 1.70 between 400nm 700nm with a maximum error of 0.0036 units (0.24% of actual) at 414nm for a = 1.50 sample. We present system design and calibration method details as well as results from a system validation sample.

  12. DETECTION AND IDENTIFICATION OF TOXIC AIR POLLUTANTS USING AIRBORNE LWIR HYPERSPECTRAL IMAGING

    EPA Science Inventory

    Airborne longwave infrared LWIR) hyperspectral imagery was utilized to detect and identify gaseous chemical release plumes at sites in sourthern Texzas. The Airborne Hysperspectral Imager (AHI), developed by the University of Hawaii was flown over a petrochemical facility and a ...

  13. Hyperspectral imaging of neoplastic progression in a mouse model of oral carcinogenesis

    NASA Astrophysics Data System (ADS)

    Lu, Guolan; Qin, Xulei; Wang, Dongsheng; Muller, Susan; Zhang, Hongzheng; Chen, Amy; Chen, Zhuo Georgia; Fei, Baowei

    2016-03-01

    Hyperspectral imaging (HSI) is an emerging modality for medical applications and holds great potential for noninvasive early detection of cancer. It has been reported that early cancer detection can improve the survival and quality of life of head and neck cancer patients. In this paper, we explored the possibility of differentiating between premalignant lesions and healthy tongue tissue using hyperspectral imaging in a chemical induced oral cancer animal model. We proposed a novel classification algorithm for cancer detection using hyperspectral images. The method detected the dysplastic tissue with an average area under the curve (AUC) of 0.89. The hyperspectral imaging and classification technique may provide a new tool for oral cancer detection.

  14. Development of algorithms for detection of mechanical injury on white mushrooms (Agaricus bisporus) using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Gowen, A. A.; O'Donnell, C. P.

    2009-05-01

    White mushrooms were subjected to mechanical injury by controlled shaking in a plastic box at 400 rpm for different times (0, 60, 120, 300 and 600 s). Immediately after shaking, hyperspectral images were obtained using two pushbroom line-scanning hyperspectral imaging instruments, one operating in the wavelength range of 400 - 1000 nm with spectroscopic resolution of 5 nm, the other operating in the wavelength range of 950 - 1700 nm with spectroscopic resolution of 7 nm. Different spectral and spatial pretreatments were investigated to reduce the effect of sample curvature on hyperspectral data. Algorithms based on Chemometric techniques (Principal Component Analysis and Partial Least Squares Discriminant Analysis) and image processing methods (masking, thresholding, morphological operations) were developed for pixel classification in hyperspectral images. In addition, correlation analysis, spectral angle mapping and scaled difference of sample spectra were investigated and compared with the chemometric approaches.

  15. DETECTION OF BACTERIAL BIOFILM ON STAINLESS STEEL BY HYPERSPECTRAL FLUORESCENCE IMAGING

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this study, hyperspectral fluorescence imaging techniques were investigated for detection of microbial biofilm on stainless steel plates typically used to manufacture food processing equipment. Stainless steel coupons were immersed in bacterium cultures consisting of nonpathogenic E. coli, Pseudo...

  16. Rapid prototyping of biomimetic vascular phantoms for hyperspectral reflectance imaging.

    PubMed

    Ghassemi, Pejhman; Wang, Jianting; Melchiorri, Anthony J; Ramella-Roman, Jessica C; Mathews, Scott A; Coburn, James C; Sorg, Brian S; Chen, Yu; Pfefer, T Joshua

    2015-01-01

    The emerging technique of rapid prototyping with three-dimensional (3-D) printers provides a simple yet revolutionary method for fabricating objects with arbitrary geometry. The use of 3-D printing for generating morphologically biomimetic tissue phantoms based on medical images represents a potentially major advance over existing phantom approaches. Toward the goal of image-defined phantoms, we converted a segmented fundus image of the human retina into a matrix format and edited it to achieve a geometry suitable for printing. Phantoms with vessel-simulating channels were then printed using a photoreactive resin providing biologically relevant turbidity, as determined by spectrophotometry. The morphology of printed vessels was validated by x-ray microcomputed tomography. Channels were filled with hemoglobin (Hb) solutions undergoing desaturation, and phantoms were imaged with a near-infrared hyperspectral reflectance imaging system. Additionally, a phantom was printed incorporating two disjoint vascular networks at different depths, each filled with Hb solutions at different saturation levels. Light propagation effects noted during these measurements—including the influence of vessel density and depth on Hb concentration and saturation estimates, and the effect of wavelength on vessel visualization depth—were evaluated. Overall, our findings indicated that 3-D-printed biomimetic phantoms hold significant potential as realistic and practical tools for elucidating light–tissue interactions and characterizing biophotonic system performance. PMID:26662064

  17. Rapid prototyping of biomimetic vascular phantoms for hyperspectral reflectance imaging

    NASA Astrophysics Data System (ADS)

    Ghassemi, Pejhman; Wang, Jianting; Melchiorri, Anthony J.; Ramella-Roman, Jessica C.; Mathews, Scott A.; Coburn, James C.; Sorg, Brian S.; Chen, Yu; Joshua Pfefer, T.

    2015-12-01

    The emerging technique of rapid prototyping with three-dimensional (3-D) printers provides a simple yet revolutionary method for fabricating objects with arbitrary geometry. The use of 3-D printing for generating morphologically biomimetic tissue phantoms based on medical images represents a potentially major advance over existing phantom approaches. Toward the goal of image-defined phantoms, we converted a segmented fundus image of the human retina into a matrix format and edited it to achieve a geometry suitable for printing. Phantoms with vessel-simulating channels were then printed using a photoreactive resin providing biologically relevant turbidity, as determined by spectrophotometry. The morphology of printed vessels was validated by x-ray microcomputed tomography. Channels were filled with hemoglobin (Hb) solutions undergoing desaturation, and phantoms were imaged with a near-infrared hyperspectral reflectance imaging system. Additionally, a phantom was printed incorporating two disjoint vascular networks at different depths, each filled with Hb solutions at different saturation levels. Light propagation effects noted during these measurements-including the influence of vessel density and depth on Hb concentration and saturation estimates, and the effect of wavelength on vessel visualization depth-were evaluated. Overall, our findings indicated that 3-D-printed biomimetic phantoms hold significant potential as realistic and practical tools for elucidating light-tissue interactions and characterizing biophotonic system performance.

  18. HIL range performance of notional hyperspectral imaging sensors

    NASA Astrophysics Data System (ADS)

    Hodgkin, Van A.; Howell, Christopher L.

    2016-05-01

    In the use of conventional broadband imaging systems, whether reflective or emissive, scene image contrasts are often so low that target discrimination is difficult or uncertain, and it is contrast that drives human-in-the-loop (HIL) sensor range performance. This situation can occur even when the spectral shapes of the target and background signatures (radiances) across the sensor waveband differ significantly from each other. The fundamental components of broadband image contrast are the spectral integrals of the target and background signatures, and this spectral integration can average away the spectral differences between scene objects. In many low broadband image contrast situations, hyperspectral imaging (HSI) can preserve a greater degree of the intrinsic scene spectral contrast for the display, and more display contrast means greater range performance by a trained observer. This paper documents a study using spectral radiometric signature modeling and the U.S. Army's Night Vision Integrated Performance Model (NV-IPM) to show how waveband selection by a notional HSI sensor using spectral contrast optimization can significantly increase HIL sensor range performance over conventional broadband sensors.

  19. Estimating foliar nitrogen concentration with hyperspectral remote sensing image

    NASA Astrophysics Data System (ADS)

    Zhang, Xia; Zhang, Bing; Liu, Liangyun; Wang, Jihua

    2003-06-01

    The hyperspectral image used in this study was acquired by the airborne operative modular imaging spectrometer (OMIS) in Xiaotangshan area, Beijing, on April 26th, 2001. Accurate geometry correction and reflectance transformation was conducted on this image so that 43 image spectra were extracted to match with the canopy-level total nitrogen concentration (TN) of wheat precisely. By using methods of stepwise regression and spectrum feature analysis, characteristic bands and parameters were selected and developed for TN retrieval from the image spectra. Nitrogen distribution map was obtained by applying the best estimation equation to all wheat pixels. It turned out, the absorption depths and areas within spectral ranges 590-756nm,1096-1295nm and 1295-1642nm could be used to estimate TN. NDVI(NRCA1175.8,NRCA733.9) and NDVI(dr745,dr699.2) was the best estimator of TN (R2 = 0.8145 and 0.769 respectively). In addition, the value and distribution of TN map was quite consistent with the field measurements and growth status.

  20. A low cost thermal infrared hyperspectral imager for small satellites

    NASA Astrophysics Data System (ADS)

    Crites, S. T.; Lucey, P. G.; Wright, R.; Garbeil, H.; Horton, K. A.

    2011-06-01

    The traditional model for space-based earth observations involves long mission times, high cost, and long development time. Because of the significant time and monetary investment required, riskier instrument development missions or those with very specific scientific goals are unlikely to successfully obtain funding. However, a niche for earth observations exploiting new technologies in focused, short lifetime missions is opening with the growth of the small satellite market and launch opportunities for these satellites. These low-cost, short-lived missions provide an experimental platform for testing new sensor technologies that may transition to larger, more long-lived platforms. The low costs and short lifetimes also increase acceptable risk to sensors, enabling large decreases in cost using commercial off the shelf (COTS) parts and allowing early-career scientists and engineers to gain experience with these projects. We are building a low-cost long-wave infrared spectral sensor, funded by the NASA Experimental Project to Stimulate Competitive Research program (EPSCOR), to demonstrate the ways in which a university's scientific and instrument development programs can fit into this niche. The sensor is a low-mass, power efficient thermal hyperspectral imager with electronics contained in a pressure vessel to enable the use of COTS electronics, and will be compatible with small satellite platforms. The sensor, called Thermal Hyperspectral Imager (THI), is based on a Sagnac interferometer and uses an uncooled 320x256 microbolometer array. The sensor will collect calibrated radiance data at long-wave infrared (LWIR, 8-14 microns) wavelengths in 230-meter pixels with 20 wavenumber spectral resolution from a 400-km orbit.

  1. Detection of single graves by airborne hyperspectral imaging.

    PubMed

    Leblanc, G; Kalacska, M; Soffer, R

    2014-12-01

    Airborne hyperspectral imaging (HSI) was assessed as a potential tool to locate single grave sites. While airborne HSI has shown to be useful to locate mass graves, it is expected the location of single graves would be an order of magnitude more difficult due to the smaller size and reduced mass of the targets. Two clearings were evaluated (through a blind test) as potential sites for containing at least one set of buried remains. At no time prior to submitting the locations of the potential burial sites from the HSI were the actual locations of the sites released or shared with anyone from the analysis team. The two HSI sensors onboard the aircraft span the range of 408-2524nm. A range of indicators that exploit the narrow spectral and spatial resolutions of the two complimentary HSI sensors onboard the aircraft were calculated. Based on the co-occurrence of anomalous pixels within the expected range of the indicators three potential areas conforming to our underlying assumptions of the expected spectral responses (and spatial area) were determined. After submission of the predicted burial locations it was revealed that two of the targets were located within GPS error (10m) of the true burial locations. Furthermore, due to the history of the TPOF site for burial work, investigation of the third target is being considered in the near future. The results clearly demonstrate promise for hyperspectral imaging to aid in the detection of buried remains, however further work is required before these results can justifiably be used in routine scenarios. PMID:25447169

  2. Assessing pasture quality and degradation status using hyperspectral imaging: a case study from western Tibet

    NASA Astrophysics Data System (ADS)

    Lehnert, Lukas W.; Meyer, Hanna; Meyer, Nele; Reudenbach, Christoph; Bendix, Jörg

    2013-10-01

    Alpine grasslands on the Tibetan Plateau (TP) are suffering from pasture degradation induced by over-grazing, climate change and improper livestock management. Meanwhile, the status of pastures is largely unknown especially in poor accessible western parts on the TP. The aim of this case study was to assess the suitability of hyperspectral imaging to predict quality and amount of forage on the western TP. Therefore, 18 ground- based hyperspectral images taken along two transects on a winter pasture were used to estimate leaf chlorophyll content, photosynthetic-active vegetation cover (PV) and proportion of grasses. For calibration and validation purposes, chlorophyll content of 20 grass plants was measured in situ. From the images reference spectra of grass and non-grass species were collected. PV was assessed from similarity of images to mean vegetation spectra using spectral angle mapper and threshold classifications. A set of 48 previously published hyperspectral vegetation indices (VI) was used as predictors to estimate chlorophyll content and to discriminate grass and non-grass pixels. Separation into grass and non-grass species was performed using partial least squares (PLS) discriminant analysis and chlorophyll content was estimated with PLS regression. The accuracy of the models was assessed with leave-one-out cross validation and normalised root mean square errors (nRMSE) for chlorophyll and contingency matrices for grass classification and total PV separation. Highest error rates were observed for discrimination between vegetated and non-vegetated parts (Overall accuracy = 0.85), whilst accuracies of grass and non grass separation (Overall accuracy = 0.98) and chlorophyll estimation were higher (nRMSE = 10.7).

  3. Principles and applications of hyperspectral imaging in quality evaluation of agro-food products: a review.

    PubMed

    Elmasry, Gamal; Kamruzzaman, Mohammed; Sun, Da-Wen; Allen, Paul

    2012-01-01

    The requirements of reliability, expeditiousness, accuracy, consistency, and simplicity for quality assessment of food products encouraged the development of non-destructive technologies to meet the demands of consumers to obtain superior food qualities. Hyperspectral imaging is one of the most promising techniques currently investigated for quality evaluation purposes in numerous sorts of applications. The main advantage of the hyperspectral imaging system is its aptitude to incorporate both spectroscopy and imaging techniques not only to make a direct assessment of different components simultaneously but also to locate the spatial distribution of such components in the tested products. Associated with multivariate analysis protocols, hyperspectral imaging shows a convinced attitude to be dominated in food authentication and analysis in future. The marvellous potential of the hyperspectral imaging technique as a non-destructive tool has driven the development of more sophisticated hyperspectral imaging systems in food applications. The aim of this review is to give detailed outlines about the theory and principles of hyperspectral imaging and to focus primarily on its applications in the field of quality evaluation of agro-food products as well as its future applicability in modern food industries and research. PMID:22823348

  4. Hyperspectral fluorescence imaging coupled with multivariate image analysis techniques for contaminant screening of leafy greens

    NASA Astrophysics Data System (ADS)

    Everard, Colm D.; Kim, Moon S.; Lee, Hoyoung

    2014-05-01

    The production of contaminant free fresh fruit and vegetables is needed to reduce foodborne illnesses and related costs. Leafy greens grown in the field can be susceptible to fecal matter contamination from uncontrolled livestock and wild animals entering the field. Pathogenic bacteria can be transferred via fecal matter and several outbreaks of E.coli O157:H7 have been associated with the consumption of leafy greens. This study examines the use of hyperspectral fluorescence imaging coupled with multivariate image analysis to detect fecal contamination on Spinach leaves (Spinacia oleracea). Hyperspectral fluorescence images from 464 to 800 nm were captured; ultraviolet excitation was supplied by two LED-based line light sources at 370 nm. Key wavelengths and algorithms useful for a contaminant screening optical imaging device were identified and developed, respectively. A non-invasive screening device has the potential to reduce the harmful consequences of foodborne illnesses.

  5. Mosaicing of Hyperspectral Images: The Application of a Spectrograph Imaging Device

    PubMed Central

    Moroni, Monica; Dacquino, Carlo; Cenedese, Antonio

    2012-01-01

    Hyperspectral monitoring of large areas (more than 10 km2) can be achieved via the use of a system employing spectrometers and CMOS cameras. A robust and efficient algorithm for automatically combining multiple, overlapping images of a scene to form a single composition (i.e., for the estimation of the point-to-point mapping between views), which uses only the information contained within the images themselves is described here. The algorithm, together with the 2D fast Fourier transform, provides an estimate of the displacement between pairs of images by accounting for rotations and changes of scale. The resulting mosaic was successively georeferenced within the WGS-84 geographic coordinate system. This paper also addresses how this information can be transferred to a push broom type spectral imaging device to build the hyperspectral cube of the area prior to land classification. The performances of the algorithm were evaluated using sample images and image sequences acquired during a proximal sensing field campaign conducted in San Teodoro (Olbia-Tempio—Sardinia). The hyperspectral cube closely corresponds to the mosaic. Mapping allows for the identification of objects within the image and agrees well with ground-truth measurements. PMID:23112597

  6. [Prediction of Encapsulation Temperatures of Copolymer Films in Photovoltaic Cells Using Hyperspectral Imaging Techniques and Chemometrics].

    PubMed

    Lin, Ping; Chen, Yong-ming; Yao, Zhi-lei

    2015-11-01

    A novel method of combination of the chemometrics and the hyperspectral imaging techniques was presented to detect the temperatures of Ethylene-Vinyl Acetate copolymer (EVA) films in photovoltaic cells during the thermal encapsulation process. Four varieties of the EVA films which had been heated at the temperatures of 128, 132, 142 and 148 °C during the photovoltaic cells production process were used for investigation in this paper. These copolymer encapsulation films were firstly scanned by the hyperspectral imaging equipment (Spectral Imaging Ltd. Oulu, Finland). The scanning band range of hyperspectral equipemnt was set between 904.58 and 1700.01 nm. The hyperspectral dataset of copolymer films was randomly divided into two parts for the training and test purpose. Each type of the training set and test set contained 90 and 10 instances, respectively. The obtained hyperspectral images of EVA films were dealt with by using the ENVI (Exelis Visual Information Solutions, USA) software. The size of region of interest (ROI) of each obtained hyperspectral image of EVA film was set as 150 x 150 pixels. The average of reflectance hyper spectra of all the pixels in the ROI was used as the characteristic curve to represent the instance. There kinds of chemometrics methods including partial least squares regression (PLSR), multi-class support vector machine (SVM) and large margin nearest neighbor (LMNN) were used to correlate the characteristic hyper spectra with the encapsulation temperatures of of copolymer films. The plot of weighted regression coefficients illustrated that both bands of short- and long-wave near infrared hyperspectral data contributed to enhancing the prediction accuracy of the forecast model. Because the attained reflectance hyperspectral data of EVA materials displayed the strong nonlinearity, the prediction performance of linear modeling method of PLSR declined and the prediction precision only reached to 95%. The kernel-based forecast models were

  7. Hyperspectral Imaging for Determining Pigment Contents in Cucumber Leaves in Response to Angular Leaf Spot Disease

    PubMed Central

    Zhao, Yan-Ru; Li, Xiaoli; Yu, Ke-Qiang; Cheng, Fan; He, Yong

    2016-01-01

    Hyperspectral imaging technique was employed to determine spatial distributions of chlorophyll (Chl), and carotenoid (Car) contents in cucumber leaves in response to angular leaf spot (ALS). Altogether, 196 hyperspectral images of cucumber leaves with five infection severities of ALS were captured by a hyperspectral imaging system in the range of 380–1,030 nm covering 512 wavebands. Mean spectrum were extracted from regions of interest (ROIs) in the hyperspectral images. Partial least square regression (PLSR) models were used to develop quantitative analysis between the spectra and the pigment contents measured by biochemical analyses. In addition, regression coefficients (RCs) in PLSR models were employed to select important wavelengths (IWs) for modelling. It was found that the PLSR models developed by the IWs provided the optimal measurement results with correlation coefficient (R) of prediction of 0.871 and 0.876 for Chl and Car contents, respectively. Finally, Chl and Car distributions in cucumber leaves with the ALS infection were mapped by applying the optimal models pixel-wise to the hyperspectral images. The results proved the feasibility of hyperspectral imaging for visualizing the pigment distributions in cucumber leaves in response to ALS. PMID:27283050

  8. Hyperspectral Imaging for Determining Pigment Contents in Cucumber Leaves in Response to Angular Leaf Spot Disease.

    PubMed

    Zhao, Yan-Ru; Li, Xiaoli; Yu, Ke-Qiang; Cheng, Fan; He, Yong

    2016-01-01

    Hyperspectral imaging technique was employed to determine spatial distributions of chlorophyll (Chl), and carotenoid (Car) contents in cucumber leaves in response to angular leaf spot (ALS). Altogether, 196 hyperspectral images of cucumber leaves with five infection severities of ALS were captured by a hyperspectral imaging system in the range of 380-1,030 nm covering 512 wavebands. Mean spectrum were extracted from regions of interest (ROIs) in the hyperspectral images. Partial least square regression (PLSR) models were used to develop quantitative analysis between the spectra and the pigment contents measured by biochemical analyses. In addition, regression coefficients (RCs) in PLSR models were employed to select important wavelengths (IWs) for modelling. It was found that the PLSR models developed by the IWs provided the optimal measurement results with correlation coefficient (R) of prediction of 0.871 and 0.876 for Chl and Car contents, respectively. Finally, Chl and Car distributions in cucumber leaves with the ALS infection were mapped by applying the optimal models pixel-wise to the hyperspectral images. The results proved the feasibility of hyperspectral imaging for visualizing the pigment distributions in cucumber leaves in response to ALS. PMID:27283050

  9. On combining spectral and spatial information of hyperspectral image for camouflaged target detecting

    NASA Astrophysics Data System (ADS)

    Hua, Wenshen; Liu, Xun; Yang, Jia

    2013-12-01

    Detecting enemy's targets and being undetectable play increasingly important roles in modern warfare. Hyperspectral images can provide large spectral range and high spectral resolution, which are invaluable in discriminating between camouflaged targets and backgrounds. As supervised classification requires prior knowledge which cannot be acquired easily, unsupervised classification usually is adopted to process hyperspectral images to detect camouflaged target. But one of its drawbacks—low detecting accuracy confines its application for camouflaged target detecting. Most research on the processing of hyperspectral image tends to focus exclusively on spectral domain and ignores spatial domain. However current hyperspectral image provides high spatial resolution which contains useful information for camouflaged target detecting. A new method combining spectral and spatial information is proposed to increase the detecting accuracy using unsupervised classification. The method has two steps. In the first step, a traditional unsupervised classifier (i.e. K-MEANS, ISODATA) is adopted to classify the hyperspectral image to acquire basic classifications or clusters. During the second step, a 3×3 model and spectral angle mapping are utilized to test the spatial character of the hyperspectral image. The spatial character is defined as spatial homogeneity and calculated by spectral angle mapping. Theory analysis and experiment shows the method is reasonable and efficient. Camouflaged targets are extracted from the background and different camouflaged targets are also recognized. And the proposed algorithm outperforms K-MEANS in terms of detecting accuracy, robustness and edge's distinction. This paper demonstrates the new method is meaningful to camouflaged targets detecting.

  10. Interactive transmission of spectrally wavelet-transformed hyperspectral images

    NASA Astrophysics Data System (ADS)

    Monteagudo-Pereira, José Lino; Bartrina-Rapesta, Joan; Aulí-Llinàs, Francesc; Serra-Sagristà, Joan; Zabala, Alaitz; Pons, Xavier

    2008-08-01

    The size of images used in remote sensing scenarios has constantly increased in the last years. Remote sensing images are not only stored, but also processed and transmitted, raising the need for more resources and bandwidth. On another side, hyperspectral remote sensing images have a large number of components with a significant inter-component redundancy, which is usually taken into account by many image coding systems to improve the coding performance. The main approaches used to decorrelate the spectral dimension are the Karhunen Loeve-Transform and the Discrete Wavelet Transform (DWT). This paper is focused on DWT decorrelators because they have a lower computational complexity, and because they provide interesting features such as component and resolution scalability and progressive transmission. Influence of the spectral transform is investigated, considering the DWT kernel applied and the number of decomposition levels. In addition, a JPIP compliant application, CADI, is introduced. It may be useful to test new protocols, techniques, or coding systems, without requiring significant changes on the application. CADI can be run in most computer platforms and devices thanks to the use of JAVA and the configuration of a light-version, suitable for devices with constrained resources.

  11. Determination of pasture quality using airborne hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Pullanagari, R. R.; Kereszturi, G.; Yule, Ian J.; Irwin, M. E.

    2015-10-01

    Pasture quality is a critical determinant which influences animal performance (live weight gain, milk and meat production) and animal health. Assessment of pasture quality is therefore required to assist farmers with grazing planning and management, benchmarking between seasons and years. Traditionally, pasture quality is determined by field sampling which is laborious, expensive and time consuming, and the information is not available in real-time. Hyperspectral remote sensing has potential to accurately quantify biochemical composition of pasture over wide areas in great spatial detail. In this study an airborne imaging spectrometer (AisaFENIX, Specim) was used with a spectral range of 380-2500 nm with 448 spectral bands. A case study of a 600 ha hill country farm in New Zealand is used to illustrate the use of the system. Radiometric and atmospheric corrections, along with automatized georectification of the imagery using Digital Elevation Model (DEM), were applied to the raw images to convert into geocoded reflectance images. Then a multivariate statistical method, partial least squares (PLS), was applied to estimate pasture quality such as crude protein (CP) and metabolisable energy (ME) from canopy reflectance. The results from this study revealed that estimates of CP and ME had a R2 of 0.77 and 0.79, and RMSECV of 2.97 and 0.81 respectively. By utilizing these regression models, spatial maps were created over the imaged area. These pasture quality maps can be used for adopting precision agriculture practices which improves farm profitability and environmental sustainability.

  12. Methods for Improving Image Quality and Reducing Data Load of NIR Hyperspectral Images

    PubMed Central

    Firtha, Ferenc; Fekete, András; Kaszab, Tímea; Gillay, Bíborka; Nogula-Nagy, Médea; Kovács, Zoltán; Kantor, David B.

    2008-01-01

    Near Infrared Hyperspectral Imaging (NIRHSI) is an emerging technology platform that integrates conventional imaging and spectroscopy to attain both spatial and spectral information from an object. Two important problems in NIRHSI are those of data load and unserviceable pixels in the NIR sensor. Hyperspectral imaging experiments generate large amounts of data (typically > 50 MB per image), which tend to overwhelm the memory capacity of conventional computer systems. This inhibits the utilisation of NIRHSI for routine online industrial application. In general, approximately 1% of pixels in NIR detectors are unserviceable or ‘dead’, containing no useful information. While this percentage of pixels is insignificant for single wavelength imaging, the problem is amplified in NIRHSI, where > 100 wavelength images are typically acquired. This paper describes an approach for reducing the data load of hyperspectral experiments by using sample-specific vector-to-scalar operators for real time feature extraction and a systematic procedure for compensating for ‘dead’ pixels in the NIR sensor. The feasibility of this approach was tested for prediction of moisture content in carrot tissue.

  13. Geometric correction method of rotary scanning hyperspectral image in agriculture application

    NASA Astrophysics Data System (ADS)

    Wan, Peng; Yang, Guijun; Xu, Bo; Feng, Haikuan; Yu, Haiyang

    2015-04-01

    In order to meet the demand of farmland plot experiments hyperspectral images acquisition, an equipment that incorporating an aerial lift vehicle with hyperspectral imager was proposed. In this manner, high spatial resolution (in millimeter) imageries were collected, which meets the need of spatial resolution on farm experiments, but also improves the efficiency of image acquisition. In allusion to the image circular geometric distortion which produced by telescopic arm rotation, an image rectification method that based on mounted position and orientation system was proposed. Experimental results shows that the image rectification method is effective.

  14. A hyperspectral image optimizing method based on sub-pixel MTF analysis

    NASA Astrophysics Data System (ADS)

    Wang, Yun; Li, Kai; Wang, Jinqiang; Zhu, Yajie

    2015-04-01

    Hyperspectral imaging is used to collect tens or hundreds of images continuously divided across electromagnetic spectrum so that the details under different wavelengths could be represented. A popular hyperspectral imaging methods uses a tunable optical band-pass filter settled in front of the focal plane to acquire images of different wavelengths. In order to alleviate the influence of chromatic aberration in some segments in a hyperspectral series, in this paper, a hyperspectral optimizing method uses sub-pixel MTF to evaluate image blurring quality was provided. This method acquired the edge feature in the target window by means of the line spread function (LSF) to calculate the reliable position of the edge feature, then the evaluation grid in each line was interpolated by the real pixel value based on its relative position to the optimal edge and the sub-pixel MTF was used to analyze the image in frequency domain, by which MTF calculation dimension was increased. The sub-pixel MTF evaluation was reliable, since no image rotation and pixel value estimation was needed, and no artificial information was introduced. With theoretical analysis, the method proposed in this paper is reliable and efficient when evaluation the common images with edges of small tilt angle in real scene. It also provided a direction for the following hyperspectral image blurring evaluation and the real-time focal plane adjustment in real time in related imaging system.

  15. Hyperspectral image analysis for the determination of alteration minerals in geothermal fields: Çürüksu (Denizli) Graben, Turkey

    NASA Astrophysics Data System (ADS)

    Uygur, Merve; Karaman, Muhittin; Kumral, Mustafa

    2016-04-01

    Çürüksu (Denizli) Graben hosts various geothermal fields such as Kızıldere, Yenice, Gerali, Karahayıt, and Tekkehamam. Neotectonic activities, which are caused by extensional tectonism, and deep circulation in sub-volcanic intrusions are heat sources of hydrothermal solutions. The temperature of hydrothermal solutions is between 53 and 260 degree Celsius. Phyllic, argillic, silicic, and carbonatization alterations and various hydrothermal minerals have been identified in various research studies of these areas. Surfaced hydrothermal alteration minerals are one set of potential indicators of geothermal resources. Developing the exploration tools to define the surface indicators of geothermal fields can assist in the recognition of geothermal resources. Thermal and hyperspectral imaging and analysis can be used for defining the surface indicators of geothermal fields. This study tests the hypothesis that hyperspectral image analysis based on EO-1 Hyperion images can be used for the delineation and definition of surfaced hydrothermal alteration in geothermal fields. Hyperspectral image analyses were applied to images covering the geothermal fields whose alteration characteristic are known. To reduce data dimensionality and identify spectral endmembers, Kruse's multi-step process was applied to atmospherically and geometrically-corrected hyperspectral images. Minimum Noise Fraction was used to reduce the spectral dimensions and isolate noise in the images. Extreme pixels were identified from high order MNF bands using the Pixel Purity Index. n-Dimensional Visualization was utilized for unique pixel identification. Spectral similarities between pixel spectral signatures and known endmember spectrum (USGS Spectral Library) were compared with Spectral Angle Mapper Classification. EO-1 Hyperion hyperspectral images and hyperspectral analysis are sensitive to hydrothermal alteration minerals, as their diagnostic spectral signatures span the visible and shortwave

  16. Hyperspectral fluorescence imaging with multi wavelength LED excitation

    NASA Astrophysics Data System (ADS)

    Luthman, A. Siri; Dumitru, Sebastian; Quirós-Gonzalez, Isabel; Bohndiek, Sarah E.

    2016-04-01

    Hyperspectral imaging (HSI) can combine morphological and molecular information, yielding potential for real-time and high throughput multiplexed fluorescent contrast agent imaging. Multiplexed readout from targets, such as cell surface receptors overexpressed in cancer cells, could improve both sensitivity and specificity of tumor identification. There remains, however, a need for compact and cost effective implementations of the technology. We have implemented a low-cost wide-field multiplexed fluorescence imaging system, which combines LED excitation at 590, 655 and 740 nm with a compact commercial solid state HSI system operating in the range 600 - 1000 nm. A key challenge for using reflectance-based HSI is the separation of contrast agent fluorescence from the reflectance of the excitation light. Here, we illustrate how it is possible to address this challenge in software, using two offline reflectance removal methods, prior to least-squares spectral unmixing. We made a quantitative comparison of the methods using data acquired from dilutions of contrast agents prepared in well-plates. We then established the capability of our HSI system for non-invasive in vivo fluorescence imaging in small animals using the optimal reflectance removal method. The HSI presented here enables quantitative unmixing of at least four fluorescent contrast agents (Alexa Fluor 610, 647, 700 and 750) simultaneously in living mice. A successful unmixing of the four fluorescent contrast agents was possible both using the pure contrast agents and with mixtures. The system could in principle also be applied to imaging of ex vivo tissue or intraoperative imaging in a clinical setting. These data suggest a promising approach for developing clinical applications of HSI based on multiplexed fluorescence contrast agent imaging.

  17. Development of a hyperspectral fluorescence lifetime imaging microscope and its application to tissue imaging

    NASA Astrophysics Data System (ADS)

    Owen, Dylan M.; Manning, Hugh B.; de Beule, Pieter; Talbot, Clifford; Requejo-Isidro, Jose; Dunsby, Chris; McGinty, James; Benninger, Richard K. P.; Elson, Dan S.; Munro, Ian; Galletly, Neil P.; Lever, M. Jon; Stamp, Gordon W.; Anand, Praveen; Neil, Mark A. A.; French, Paul M. W.

    2007-02-01

    We present the design, characterization and application of a novel, rapid, optically sectioned hyperspectral fluorescence lifetime imaging (FLIM) microscope. The system is based on a line scanning confocal configuration and uses a highspeed time-gated detector to extract lifetime information from many pixels in parallel. This allows the full spectraltemporal profiles of a fluorescence decay to be obtained from every pixel in an image. Line illumination and slit detection also gives the microscope a confocal optical sectioning ability. The system is applied to test samples and unstained biological tissue. In future, this microscope will be combined with recently-developed continuously electronically tunable, pulsed light sources based on tapered, micro-structured optical fibers. This will allow hyperspectral FLIM to be combined with the advantages of excitation spectroscopy to gain further insight into complex biological specimens including tissue and live cell imaging.

  18. Compressive fluorescence microscopy for biological and hyperspectral imaging.

    PubMed

    Studer, Vincent; Bobin, Jérome; Chahid, Makhlad; Mousavi, Hamed Shams; Candes, Emmanuel; Dahan, Maxime

    2012-06-26

    The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware implementations of CS-based acquisition devices--especially in optics--have only started being addressed. This paper presents an implementation of compressive sensing in fluorescence microscopy and its applications to biomedical imaging. Our CS microscope combines a dynamic structured wide-field illumination and a fast and sensitive single-point fluorescence detection to enable reconstructions of images of fluorescent beads, cells, and tissues with undersampling ratios (between the number of pixels and number of measurements) up to 32. We further demonstrate a hyperspectral mode and record images with 128 spectral channels and undersampling ratios up to 64, illustrating the potential benefits of CS acquisition for higher-dimensional signals, which typically exhibits extreme redundancy. Altogether, our results emphasize the interest of CS schemes for acquisition at a significantly reduced rate and point to some remaining challenges for CS fluorescence microscopy. PMID:22689950

  19. Hyperspectral Imaging Using Flexible Endoscopy for Laryngeal Cancer Detection.

    PubMed

    Regeling, Bianca; Thies, Boris; Gerstner, Andreas O H; Westermann, Stephan; Müller, Nina A; Bendix, Jörg; Laffers, Wiebke

    2016-01-01

    Hyperspectral imaging (HSI) is increasingly gaining acceptance in the medical field. Up until now, HSI has been used in conjunction with rigid endoscopy to detect cancer in vivo. The logical next step is to pair HSI with flexible endoscopy, since it improves access to hard-to-reach areas. While the flexible endoscope's fiber optic cables provide the advantage of flexibility, they also introduce an interfering honeycomb-like pattern onto images. Due to the substantial impact this pattern has on locating cancerous tissue, it must be removed before the HS data can be further processed. Thereby, the loss of information is to minimize avoiding the suppression of small-area variations of pixel values. We have developed a system that uses flexible endoscopy to record HS cubes of the larynx and designed a special filtering technique to remove the honeycomb-like pattern with minimal loss of information. We have confirmed its feasibility by comparing it to conventional filtering techniques using an objective metric and by applying unsupervised and supervised classifications to raw and pre-processed HS cubes. Compared to conventional techniques, our method successfully removes the honeycomb-like pattern and considerably improves classification performance, while preserving image details. PMID:27529255

  20. Hyper-spectral imaging of aircraft exhaust plumes

    NASA Astrophysics Data System (ADS)

    Bowen, Spencer; Bradley, Kenneth; Gross, Kevin; Perram, Glen; Marciniak, Michael

    2008-10-01

    An imaging Fourier-transform spectrometer has been used to determine low spatial resolution temperature and chemical species concentration distributions of aircraft jet engine exhaust plumes. An overview of the imaging Fourier transform spectrometer and the methodology of the project is presented. Results to date are shared and future work is discussed. Exhaust plume data from a Turbine Technologies, LTD, SR-30 turbojet engine at three engine settings was collected using a Telops Field-portable Imaging Radiometric Spectrometer Technology Mid-Wave Extended (FIRST-MWE). Although the plume exhibited high temporal frequency fluctuations, temporal averaging of hyper-spectral data-cubes produced steady-state distributions, which, when co-added and Fourier transformed, produced workable spectra. These spectra were then reduced using a simplified gaseous effluent model to fit forward-modeled spectra obtained from the Line-By-Line Radiative Transfer Model (LBLRTM) and the high-resolution transmission (HITRAN) molecular absorption database to determine approximate temperature and concentration distributions. It is theorized that further development of the physical model will produce better agreement between measured and modeled data.

  1. Comparison of broadband and hyperspectral thermal infrared imaging of buried threat objects

    NASA Astrophysics Data System (ADS)

    McFee, John E.; Achal, Steve B.; Diaz, Alejandra U.; Faust, Anthony A.

    2013-06-01

    Previous research by many groups has shown that broad-band thermal infrared (TIR) imagers can detect buried explosive threat devices, such as unexploded ordnance (UXO), landmines and improvised explosive devices (IEDs). Broad-band detection measures the apparent temperature - an average over the wave band of the product of the true soil surface temperature and the emissivity. Broad-band detection suffers from inconsistent performance (low signal, high clutter rates), due in part to diurnal variations, environmental and meteorological conditions, and soil surface effects. It has been suggested that hyperspectral TIR imaging might have improved performance since it can, in principle, allow extraction of the wavelength-dependent emissivity and the true soil surface temperature. This would allow the surface disturbance effects to be separated from the soil column (bulk) effects. A significant, and as yet unanswered, question is whether hyperspectral TIR images provide better detection capability (higher probability of detection and/or lower false alarm rate) than do broad-band thermal images. TIR hyperspectral image data of threat objects, buried and surface-laid in bare soil, were obtained in arid, desert-like conditions over full diurnal cycles for several days. Regions of interest containing threat objects and backgrounds were extracted throughout the time period. Simulated broad-band images were derived from the hyperspectral images. The diurnal variation of the images was studied. Hyperspectral was found to provide some advantage over broad-band imaging in detection of buried threat objects for the limited data set studied.

  2. Multiplexed hyperspectral imaging and spectrometry using spatial light modulators

    NASA Astrophysics Data System (ADS)

    Deverse, Richard Andrew

    1999-11-01

    The signal-noise-ratio, (SNR) is often the primary figure of merit when evaluating instrument performance. Multiplexing is one method employed in spectrometry and imaging to increase the magnitude of a signal received by a detector during a period of measurement. Optical masks employing Hadamard encoding matrices for multiplexing can be used to increase the SNR of some spectrometric and hyperspectrometric imaging measurements. A transmissive/opaque metal-etched optical mask and a reflective micro-mirror optical mask were used to investigate Hadamard transform (HT) multiplexing in spectrometric and hyperspectrometric imaging measurements. HT spectrometry (HTS) is a combination of dispersive and multiplexing spectrometry employing a one- dimensional (1D) optical mask. Dividing and folding the ID Hadamard, encoded optical mask used for spectral encoding in HT multiplexing spectrometry results in a two-dimensional (2D) mask that can be used for spatial encoding in Hadamard transform imaging (HTI). An etched, mechanically translated 2D optical mask and a digital micro-mirror array (DMA) encoded by a cyclic S255 matrix were successfully employed in the investigation of spatial multiplexing in HT Raman chemical mapping systems. Imaging heterogeneous liquid and solid samples using a 2D Hadamard encoded metal- etched moving mask was successfully demonstrated using conventional Raman spectroscopic equipment. The DMA is an improvement in Hadamard optical mask technology over moving optical masks and was successfully applied to visible Raman hyperspectral imaging of heterogeneous liquid and solid samples. The DMA was also successfully demonstrated to be effective for improving SNR in near- infrared (NIR) FITS as a 1D Hadamard encoded optical mask.

  3. Hyperspectral imaging using a color camera and its application for pathogen detection

    NASA Astrophysics Data System (ADS)

    Yoon, Seung-Chul; Shin, Tae-Sung; Heitschmidt, Gerald W.; Lawrence, Kurt C.; Park, Bosoon; Gamble, Gary

    2015-02-01

    This paper reports the results of a feasibility study for the development of a hyperspectral image recovery (reconstruction) technique using a RGB color camera and regression analysis in order to detect and classify colonies of foodborne pathogens. The target bacterial pathogens were the six representative non-O157 Shiga-toxin producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) grown in Petri dishes of Rainbow agar. The purpose of the feasibility study was to evaluate whether a DSLR camera (Nikon D700) could be used to predict hyperspectral images in the wavelength range from 400 to 1,000 nm and even to predict the types of pathogens using a hyperspectral STEC classification algorithm that was previously developed. Unlike many other studies using color charts with known and noise-free spectra for training reconstruction models, this work used hyperspectral and color images, separately measured by a hyperspectral imaging spectrometer and the DSLR color camera. The color images were calibrated (i.e. normalized) to relative reflectance, subsampled and spatially registered to match with counterpart pixels in hyperspectral images that were also calibrated to relative reflectance. Polynomial multivariate least-squares regression (PMLR) was previously developed with simulated color images. In this study, partial least squares regression (PLSR) was also evaluated as a spectral recovery technique to minimize multicollinearity and overfitting. The two spectral recovery models (PMLR and PLSR) and their parameters were evaluated by cross-validation. The QR decomposition was used to find a numerically more stable solution of the regression equation. The preliminary results showed that PLSR was more effective especially with higher order polynomial regressions than PMLR. The best classification accuracy measured with an independent test set was about 90%. The results suggest the potential of cost-effective color imaging using hyperspectral image

  4. An approach for hyperspectral image classification utilization spatial-spectral combined kernel SVM

    NASA Astrophysics Data System (ADS)

    Wang, Hailei; Sun, Bingyun; Gui, Yuanmiao; Chen, Yanping; Zhou, Dongbo; Wu, Xuelian

    2015-12-01

    Hyperspectral images belong to high-dimensional data having a lot of redundancy information when they are directly used to classification. Support vector machine (SVM) can be employed to map hyperspectral data to high dimensional space effectively and make them linearly separable. In this paper, spectral and spatial information of hyperspectral images were used to construct SVM kernel function respectively. This paper proposed a hyperspectral image classification method utilization spatial-spectral combined kernel SVM in order to improve classification accuracy. The proposed method was used to classify AVIRIS hyperspectral images. The results demonstrated that the proposed SVM method can achieve 96.13% overall accuracy for the single category classification and 84.81% overall accuracy for multi-class classification only using ten percent of the total samples as the training samples. That is to say, the proposed method can make full use of the spectral information and spatial information of hyperspectral data, and effectively distinguish different categories compared with the traditional SVM for classification.

  5. Thermal infrared hyperspectral imaging from vehicle-carried instrumentation

    NASA Astrophysics Data System (ADS)

    Kirkland, Laurel E.; Herr, Kenneth C.; Adams, Paul M.; McAfee, John; Salisbury, John

    2002-09-01

    Stand-off identification in the field using thermal infrared spectrometers (hyperspectral) is a maturing technique for gases and aerosols. However, capabilities to identify solid-phase materials on the surface lag substantially, particularly for identification in the field without benefit of ground truth (e.g. for "denied areas"). Spectral signatures of solid phase materials vary in complex and non-intuitive ways, including non-linear variations with surface texture, particle size, and intimate mixing. Also, in contrast to airborne or satellite measurements, reflected downwelling radiance strongly affects the signature measured by field spectrometers. These complex issues can confound interpretations or cause a misidentification in the field. Problems that remain particularly obstinate are (1) low ambiguity identification when there is no accompanying ground truth (e.g. measurements of denied areas, or Mars surface by the 2003 Mars lander spectrometer); (2) real- or near real-time identification, especially when a low ambiguity answer is critical; (3) identification of intimate mixtures (e.g. two fine powders mixed together) and targets composed of very small particles (e.g. aerosol fallout dust, some tailings); and (4) identification of non-diffuse targets (e.g. smooth coatings such as paint and desert varnish), particularly when measured at a high emission angle. In most studies that focus on gas phase targets or specific manmade targets, the solid phase background signatures are called "clutter" and are thrown out. Here we discuss our field spectrometer images measured of test targets that were selected to include a range of particle sizes, diffuse, non-diffuse, high, and low reflectance materials. This study was designed to identify and improve understanding of the issues that complicate stand-off identification in the field, with a focus on developing identification capabilities to proceed without benefit of ground truth. This information allows both improved

  6. Differentiation of bacterial colonies and temporal growth patterns using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Mehrübeoglu, Mehrube; Buck, Gregory W.; Livingston, Daniel W.

    2014-09-01

    Detection and identification of bacteria are important for health and safety. Hyperspectral imaging offers the potential to capture unique spectral patterns and spatial information from bacteria which can then be used to detect and differentiate bacterial species. Here, hyperspectral imaging has been used to characterize different bacterial colonies and investigate their growth over time. Six bacterial species (Pseudomonas fluorescens, Escherichia coli, Serratia marcescens, Salmonella enterica, Staphylococcus aureus, Enterobacter aerogenes) were grown on tryptic soy agar plates. Hyperspectral data were acquired immediately after, 24 hours after, and 96 hours after incubation. Spectral signatures from bacterial colonies demonstrated repeatable measurements for five out of six species. Spatial variations as well as changes in spectral signatures were observed across temporal measurements within and among species at multiple wavelengths due to strengthening or weakening reflectance signals from growing bacterial colonies based on their pigmentation. Between-class differences and within-class similarities were the most prominent in hyperspectral data collected 96 hours after incubation.

  7. Hyperspectral imaging of gases with a continuous-wave pump-enhanced optical parametric oscillator.

    PubMed

    Stothard, David; Dunn, Malcolm; Rae, Cameron

    2004-03-01

    We demonstrate a system for the active real-time hyperspectral imaging of gases using a combination of a compact, pump-enhanced, continuous-wave optical parametric oscillator as an all-solid-state mid-infrared source of coherent radiation and an electro-mechanical polygonal imager. The wide spectral coverage and high spectral resolution characteristics of this source means that the system is capable of being selectively tuned into the absorption features of a wide variety of gaseous species. As an example we show how the largest absorption coefficient exhibited by methane at 3057.7cm(-1) can be accessed (amongst others) and gas plumes imaged in concentrations as low as 30ppm.m using a parametric oscillator based on periodically-poled RbTiOAsO(4) (PP-RTA). PMID:19474906

  8. Hyperspectral imaging of gases with a continuous-wave pump-enhanced optical parametric oscillator

    NASA Astrophysics Data System (ADS)

    Stothard, David J. M.; Dunn, Malcolm H.; Rae, Cameron F.

    2004-03-01

    We demonstrate a system for the active real-time hyperspectral imaging of gases using a combination of a compact, pump-enhanced, continuous-wave optical parametric oscillator as an all-solid-state mid-infrared source of coherent radiation and an electro-mechanical polygonal imager. The wide spectral coverage and high spectral resolution characteristics of this source means that the system is capable of being selectively tuned into the absorption features of a wide variety of gaseous species. As an example we show how the largest absorption coefficient exhibited by methane at 3057.7cm-1 can be accessed (amongst others) and gas plumes imaged in concentrations as low as 30ppm.m using a parametric oscillator based on periodically-poled RbTiOAsO4 (PP-RTA).

  9. Development and integration of Raman imaging capabilities to Sandia National Laboratories hyperspectral fluorescence imaging instrument.

    SciTech Connect

    Timlin, Jerilyn Ann; Nieman, Linda T.

    2005-11-01

    Raman spectroscopic imaging is a powerful technique for visualizing chemical differences within a variety of samples based on the interaction of a substance's molecular vibrations with laser light. While Raman imaging can provide a unique view of samples such as residual stress within silicon devices, chemical degradation, material aging, and sample heterogeneity, the Raman scattering process is often weak and thus requires very sensitive collection optics and detectors. Many commercial instruments (including ones owned here at Sandia National Laboratories) generate Raman images by raster scanning a point focused laser beam across a sample--a process which can expose a sample to extreme levels of laser light and requires lengthy acquisition times. Our previous research efforts have led to the development of a state-of-the-art two-dimensional hyperspectral imager for fluorescence imaging applications such as microarray scanning. This report details the design, integration, and characterization of a line-scan Raman imaging module added to this efficient hyperspectral fluorescence microscope. The original hyperspectral fluorescence instrument serves as the framework for excitation and sample manipulation for the Raman imaging system, while a more appropriate axial transmissive Raman imaging spectrometer and detector are utilized for collection of the Raman scatter. The result is a unique and flexible dual-modality fluorescence and Raman imaging system capable of high-speed imaging at high spatial and spectral resolutions. Care was taken throughout the design and integration process not to hinder any of the fluorescence imaging capabilities. For example, an operator can switch between the fluorescence and Raman modalities without need for extensive optical realignment. The instrument performance has been characterized and sample data is presented.

  10. Hyperspectral imaging applied to complex particulate solids systems

    NASA Astrophysics Data System (ADS)

    Bonifazi, Giuseppe; Serranti, Silvia

    2008-04-01

    HyperSpectral Imaging (HSI) is based on the utilization of an integrated hardware and software (HW&SW) platform embedding conventional imaging and spectroscopy to attain both spatial and spectral information from an object. Although HSI was originally developed for remote sensing, it has recently emerged as a powerful process analytical tool, for non-destructive analysis, in many research and industrial sectors. The possibility to apply on-line HSI based techniques in order to identify and quantify specific particulate solid systems characteristics is presented and critically evaluated. The originally developed HSI based logics can be profitably applied in order to develop fast, reliable and lowcost strategies for: i) quality control of particulate products that must comply with specific chemical, physical and biological constraints, ii) performance evaluation of manufacturing strategies related to processing chains and/or realtime tuning of operative variables and iii) classification-sorting actions addressed to recognize and separate different particulate solid products. Case studies, related to recent advances in the application of HSI to different industrial sectors, as agriculture, food, pharmaceuticals, solid waste handling and recycling, etc. and addressed to specific goals as contaminant detection, defect identification, constituent analysis and quality evaluation are described, according to authors' originally developed application.

  11. Compressive Source Separation: Theory and Methods for Hyperspectral Imaging

    NASA Astrophysics Data System (ADS)

    Golbabaee, Mohammad; Arberet, Simon; Vandergheynst, Pierre

    2013-12-01

    With the development of numbers of high resolution data acquisition systems and the global requirement to lower the energy consumption, the development of efficient sensing techniques becomes critical. Recently, Compressed Sampling (CS) techniques, which exploit the sparsity of signals, have allowed to reconstruct signal and images with less measurements than the traditional Nyquist sensing approach. However, multichannel signals like Hyperspectral images (HSI) have additional structures, like inter-channel correlations, that are not taken into account in the classical CS scheme. In this paper we exploit the linear mixture of sources model, that is the assumption that the multichannel signal is composed of a linear combination of sources, each of them having its own spectral signature, and propose new sampling schemes exploiting this model to considerably decrease the number of measurements needed for the acquisition and source separation. Moreover, we give theoretical lower bounds on the number of measurements required to perform reconstruction of both the multichannel signal and its sources. We also proposed optimization algorithms and extensive experimentation on our target application which is HSI, and show that our approach recovers HSI with far less measurements and computational effort than traditional CS approaches.

  12. Hyperspectral-imaging-based techniques applied to wheat kernels characterization

    NASA Astrophysics Data System (ADS)

    Serranti, Silvia; Cesare, Daniela; Bonifazi, Giuseppe

    2012-05-01

    Single kernels of durum wheat have been analyzed by hyperspectral imaging (HSI). Such an approach is based on the utilization of an integrated hardware and software architecture able to digitally capture and handle spectra as an image sequence, as they results along a pre-defined alignment on a surface sample properly energized. The study was addressed to investigate the possibility to apply HSI techniques for classification of different types of wheat kernels: vitreous, yellow berry and fusarium-damaged. Reflectance spectra of selected wheat kernels of the three typologies have been acquired by a laboratory device equipped with an HSI system working in near infrared field (1000-1700 nm). The hypercubes were analyzed applying principal component analysis (PCA) to reduce the high dimensionality of data and for selecting some effective wavelengths. Partial least squares discriminant analysis (PLS-DA) was applied for classification of the three wheat typologies. The study demonstrated that good classification results were obtained not only considering the entire investigated wavelength range, but also selecting only four optimal wavelengths (1104, 1384, 1454 and 1650 nm) out of 121. The developed procedures based on HSI can be utilized for quality control purposes or for the definition of innovative sorting logics of wheat.

  13. High resolution hyperspectral imaging with a high throughput virtual slit

    NASA Astrophysics Data System (ADS)

    Gooding, Edward A.; Gunn, Thomas; Cenko, Andrew T.; Hajian, Arsen R.

    2016-05-01

    Hyperspectral imaging (HSI) device users often require both high spectral resolution, on the order of 1 nm, and high light-gathering power. A wide entrance slit assures reasonable étendue but degrades spectral resolution. Spectrometers built using High Throughput Virtual Slit™ (HTVS) technology optimize both parameters simultaneously. Two remote sensing use cases that require high spectral resolution are discussed. First, detection of atmospheric gases with intrinsically narrow absorption lines, such as hydrocarbon vapors or combustion exhaust gases such as NOx and CO2. Detecting exhaust gas species with high precision has become increasingly important in the light of recent events in the automobile industry. Second, distinguishing reflected daylight from emission spectra in the visible and NIR (VNIR) regions is most easily accomplished using the Fraunhofer absorption lines in solar spectra. While ground reflectance spectral features in the VNIR are generally quite broad, the Fraunhofer lines are narrow and provide a signature of intrinsic vs. extrinsic illumination. The High Throughput Virtual Slit enables higher spectral resolution than is achievable with conventional spectrometers by manipulating the beam profile in pupil space. By reshaping the instrument pupil with reflective optics, HTVS-equipped instruments create a tall, narrow image profile at the exit focal plane, typically delivering 5X or better the spectral resolution achievable with a conventional design.

  14. Hyperspectral imaging for detection of scab in wheat

    NASA Astrophysics Data System (ADS)

    Delwiche, Stephen R.; Kim, Moon S.

    2000-12-01

    Scab (Fusarium head blight) is a disease that causes wheat kernels to be shriveled, underweight, and difficult to mill. Scab is also a health concern because of the possible concomitant production of the mycotoxin deoxynivalenol. Current official inspection procedures entail manual human inspection. A study was undertaken to explore the possibility of detecting scab-damaged wheat kernels by machine vision. A custom-made hyperspectral imaging system, possessing a wavelength range of 425 to 860 nm with neighboring bands 3.7 nm apart, a spatial resolution of 0.022 mm2/pixel, and 16-bit per pixel dynamic range, gathered images of non-touching kernels from three wheat varieties. Each variety was represented by 32 normal and 32 scab-damaged kernels. From a search of wavelengths that could be used to separate the two classes (normal vs. scab), a linear discriminant function was constructed from the best R((lambda) 1)/R((lambda) 2), based on the assumption of a multivariate normal distribution for each class and the pooling of the covariance error that averaged between 2 and 17%, dependent on wheat variety. With expansion to the testing of more varieties, a two-to-four wavelength machine vision system appears to be a feasible alternative to manual inspection.

  15. Airborne infrared hyperspectral imager for intelligence, surveillance and reconnaissance applications

    NASA Astrophysics Data System (ADS)

    Lagueux, Philippe; Puckrin, Eldon; Turcotte, Caroline S.; Gagnon, Marc-André; Bastedo, John; Farley, Vincent; Chamberland, Martin

    2012-09-01

    Persistent surveillance and collection of airborne intelligence, surveillance and reconnaissance information is critical in today's warfare against terrorism. High resolution imagery in visible and infrared bands provides valuable detection capabilities based on target shapes and temperatures. However, the spectral resolution provided by a hyperspectral imager adds a spectral dimension to the measurements, leading to additional tools for detection and identification of targets, based on their spectral signature. The Telops Hyper-Cam sensor is an interferometer-based imaging system that enables the spatial and spectral analysis of targets using a single sensor. It is based on the Fourier-transform technology yielding high spectral resolution and enabling high accuracy radiometric calibration. It provides datacubes of up to 320×256 pixels at spectral resolutions as fine as 0.25 cm-1. The LWIR version covers the 8.0 to 11.8 μm spectral range. The Hyper-Cam has been recently used for the first time in two compact airborne platforms: a bellymounted gyro-stabilized platform and a gyro-stabilized gimbal ball. Both platforms are described in this paper, and successful results of high-altitude detection and identification of targets, including industrial plumes, and chemical spills are presented.

  16. Airborne infrared hyperspectral imager for intelligence, surveillance, and reconnaissance applications

    NASA Astrophysics Data System (ADS)

    Puckrin, Eldon; Turcotte, Caroline S.; Gagnon, Marc-André; Bastedo, John; Farley, Vincent; Chamberland, Martin

    2012-06-01

    Persistent surveillance and collection of airborne intelligence, surveillance and reconnaissance information is critical in today's warfare against terrorism. High resolution imagery in visible and infrared bands provides valuable detection capabilities based on target shapes and temperatures. However, the spectral resolution provided by a hyperspectral imager adds a spectral dimension to the measurements, leading to additional tools for detection and identification of targets, based on their spectral signature. The Telops Hyper-Cam sensor is an interferometer-based imaging system that enables the spatial and spectral analysis of targets using a single sensor. It is based on the Fourier-transform technology yielding high spectral resolution and enabling high accuracy radiometric calibration. It provides datacubes of up to 320×256 pixels at spectral resolutions as fine as 0.25 cm-1. The LWIR version covers the 8.0 to 11.8 μm spectral range. The Hyper-Cam has been recently used for the first time in two compact airborne platforms: a belly-mounted gyro-stabilized platform and a gyro-stabilized gimbal ball. Both platforms are described in this paper, and successful results of high-altitude detection and identification of targets, including industrial plumes, and chemical spills are presented.

  17. Gas plume quantification in downlooking hyperspectral longwave infrared images

    NASA Astrophysics Data System (ADS)

    Turcotte, Caroline S.; Davenport, Michael R.

    2010-10-01

    Algorithms have been developed to support quantitative analysis of a gas plume using down-looking airborne hyperspectral long-wave infrared (LWIR) imagery. The resulting gas quantification "GQ" tool estimates the quantity of one or more gases at each pixel, and estimates uncertainty based on factors such as atmospheric transmittance, background clutter, and plume temperature contrast. GQ uses gas-insensitive segmentation algorithms to classify the background very precisely so that it can infer gas quantities from the differences between plume-bearing pixels and similar non-plume pixels. It also includes MODTRAN-based algorithms to iteratively assess various profiles of air temperature, water vapour, and ozone, and select the one that implies smooth emissivity curves for the (unknown) materials on the ground. GQ then uses a generalized least-squares (GLS) algorithm to simultaneously estimate the most likely mixture of background (terrain) material and foreground plume gases. Cross-linking of plume temperature to the estimated gas quantity is very non-linear, so the GLS solution was iteratively assessed over a range of plume temperatures to find the best fit to the observed spectrum. Quantification errors due to local variations in the camera-topixel distance were suppressed using a subspace projection operator. Lacking detailed depth-maps for real plumes, the GQ algorithm was tested on synthetic scenes generated by the Digital Imaging and Remote Sensing Image Generation (DIRSIG) software. Initial results showed pixel-by-pixel gas quantification errors of less than 15% for a Freon 134a plume.

  18. Hyperspectral backscatter imaging: a label-free approach to cytogenetics.

    PubMed

    Rebner, Karsten; Ostertag, Edwin; Kessler, Rudolf W

    2016-08-01

    Current techniques for chromosome analysis need to be improved for rapid, economical identification of complex chromosomal defects by sensitive and selective visualisation. In this paper, we present a straightforward method for characterising unstained human metaphase chromosomes. Backscatter imaging in a dark-field setup combined with visible and short near-infrared spectroscopy is used to monitor morphological differences in the distribution of the chromosomal fine structure in human metaphase chromosomes. The reasons for the scattering centres in the fine structure are explained. Changes in the scattering centres during preparation of the metaphases are discussed. FDTD simulations are presented to substantiate the experimental findings. We show that local scattering features consisting of underlying spectral modulations of higher frequencies associated with a high variety of densely packed chromatin can be represented by their scatter profiles even on a sub-microscopic level. The result is independent of the chromosome preparation and structure size. This analytical method constitutes a rapid, cost-effective and label-free cytogenetic technique which can be used in a standard light microscope. Graphical abstract Hyperspectral backscatter imaging for label-free characterization. PMID:27277813

  19. Detection of chemical pollutants by passive LWIR hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Lavoie, Hugo; Thériault, Jean-Marc; Bouffard, François; Puckrin, Eldon; Dubé, Denis

    2012-09-01

    Toxic industrial chemicals (TICs) represent a major threat to public health and security. Their detection constitutes a real challenge to security and first responder's communities. One promising detection method is based on the passive standoff identification of chemical vapors emanating from the laboratory under surveillance. To investigate this method, the Department of National Defense and Public Safety Canada have mandated Defense Research and Development Canada (DRDC) - Valcartier to develop and test passive Long Wave Infrared (LWIR) hyperspectral imaging (HSI) sensors for standoff detection. The initial effort was focused to address the standoff detection and identification of toxic industrial chemicals (TICs) and precursors. Sensors such as the Multi-option Differential Detection and Imaging Fourier Spectrometer (MoDDIFS) and the Improved Compact ATmospheric Sounding Interferometer (iCATSI) were developed for this application. This paper describes the sensor developments and presents initial results of standoff detection and identification of TICs and precursors. The standoff sensors are based on the differential Fourier-transform infrared (FTIR) radiometric technology and are able to detect, spectrally resolve and identify small leak plumes at ranges in excess of 1 km. Results from a series of trials in asymmetric threat type scenarios will be presented. These results will serve to establish the potential of the method for standoff detection of TICs precursors and surrogates.

  20. LWIR hyperspectral imaging application and detection of chemical precursors

    NASA Astrophysics Data System (ADS)

    Lavoie, Hugo; Thériault, Jean-Marc; Bouffard, François; Puckrin, Eldon; Dubé, Denis

    2012-10-01

    Detection and identification of Toxic industrial chemicals (TICs) represent a major challenge to protect and sustain first responder and public security. In this context, passive Hyperspectral Imaging (HSI) is a promising technology for the standoff detection and identification of chemical vapors emanating from a distant location. To investigate this method, the Department of National Defense and Public Safety Canada have mandated Defense Research and Development Canada (DRDC) - Valcartier to develop and test Very Long Wave Infrared (VLWIR) HSI sensors for standoff detection. The initial effort was focused to address the standoff detection and identification of toxic industrial chemicals (TICs), surrogates and precursors. Sensors such as the Improved Compact ATmospheric Sounding Interferometer (iCATSI) and the Multi-option Differential Detection and Imaging Fourier Spectrometer (MoDDIFS) were developed for this application. This paper presents the sensor developments and preliminary results of standoff detection and identification of TICs and precursors. The iCATSI and MoDDIFS sensors are based on the optical differential Fourier-transform infrared (FTIR) radiometric technology and are able to detect, spectrally resolve and identify small leak at ranges in excess of 1 km. Results from a series of trials in asymmetric threat type scenarios are reported. These results serve to establish the potential of passive standoff HSI detection of TICs, precursors and surrogates.

  1. Optimal Segmentation Strategy for Compact Representation of Hyperspectral Image Cubes

    SciTech Connect

    Paglieroni, D; Roberts, R

    2000-02-08

    By producing compact representations of hyperspectral image cubes (hypercubes), image storage requirements and the amount of time it takes to extract essential elements of information can both be dramatically reduced. However, these compact representations must preserve the important spectral features within hypercube pixels and the spatial structure associated with background and objects or phenomena of interest. This paper describes a novel approach for automatically and efficiently generating a particular type of compact hypercube representation, referred to as a supercube. The hypercube is segmented into regions that contain pixels with similar spectral shapes that are spatially connected, and the pixel connectivity constraint can be relaxed. Thresholds of similarity in spectral shape between pairs of pixels are derived directly from the hypercube data. One superpixel is generated for each region as some linear combination of pixels belonging to that region. The superpixels are optimal in the sense that the linear combination coefficients are computed so as to minimize the level of noise. Each hypercube pixel is represented in the supercube by applying a gain and bias to the superpixel assigned to the region containing that pixel. Examples are provided.

  2. Development of a compressive sampling hyperspectral imager prototype

    NASA Astrophysics Data System (ADS)

    Barducci, Alessandro; Guzzi, Donatella; Lastri, Cinzia; Nardino, Vanni; Marcoionni, Paolo; Pippi, Ivan

    2013-10-01

    Compressive sensing (CS) is a new technology that investigates the chance to sample signals at a lower rate than the traditional sampling theory. The main advantage of CS is that compression takes place during the sampling phase, making possible significant savings in terms of the ADC, data storage memory, down-link bandwidth, and electrical power absorption. The CS technology could have primary importance for spaceborne missions and technology, paving the way to noteworthy reductions of payload mass, volume, and cost. On the contrary, the main CS disadvantage is made by the intensive off-line data processing necessary to obtain the desired source estimation. In this paper we summarize the CS architecture and its possible implementations for Earth observation, giving evidence of possible bottlenecks hindering this technology. CS necessarily employs a multiplexing scheme, which should produce some SNR disadvantage. Moreover, this approach would necessitate optical light modulators and 2-dim detector arrays of high frame rate. This paper describes the development of a sensor prototype at laboratory level that will be utilized for the experimental assessment of CS performance and the related reconstruction errors. The experimental test-bed adopts a push-broom imaging spectrometer, a liquid crystal plate, a standard CCD camera and a Silicon PhotoMultiplier (SiPM) matrix. The prototype is being developed within the framework of the ESA ITI-B Project titled "Hyperspectral Passive Satellite Imaging via Compressive Sensing".

  3. Development of real-time line-scan hyperspectral imaging system for online agricultural and food product inspection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper reports a recent development of a line-scan hyperspectral imaging system for real-time multispectral imaging applications in agricultural and food industries. The hyperspectral imaging system consisted of a spectrograph, an EMCCD camera, and application software. The real-time multispectr...

  4. Fusion of hyperspectral images and lidar-based dems for coastal mapping

    NASA Astrophysics Data System (ADS)

    Elaksher, Ahmed F.

    2008-07-01

    Coastal mapping is essential for a variety of applications such as coastal resource management, coastal environmental protection, and coastal development and planning. Various mapping techniques, like ground and aerial surveying, have been utilized in mapping coastal areas. Recently, multispectral and hyperspectral satellite images and elevation data from active sensors have also been used in coastal mapping. Integrating these datasets can provide more reliable coastal information. This paper presents a novel technique for coastal mapping from an airborne visible/infrared imaging spectrometer (AVIRIS) hyperspectral image and a light detection and ranging (LIDAR)-based digital elevation model (DEM). The DEM was used to detect and create a vector layer for building polygons. Subsequently, building pixels were removed from the AVIRIS image and the image was classified with a supervised classifier to discriminate road and water pixels. Two vector layers for the road network and the shoreline segments were vectorized from road pixels and water-body border pixels using several image-processing algorithms. The geometric accuracy and completeness of the results were evaluated. The average positional accuracies for the building, road network, and shoreline layers were 2.3, 5.7, and 7.2 m, respectively. The detection rates of the three layers were 93.2%, 91.3%, and 95.2%, respectively. Results confirmed that utilizing laser ranging data to detect and remove buildings from optical images before the classification process enhances the outcomes of this process. Consequently, integrating laser and optical data provides high-quality and more reliable coastal geospatial information.

  5. Demonstration of the Wide-Field Imaging Interferometer Testbed Using a Calibrated Hyperspectral Image Projector

    NASA Technical Reports Server (NTRS)

    Bolcar, Matthew R.; Leisawitz, David; Maher, Steve; Rinehart, Stephen

    2012-01-01

    The Wide-field Imaging Interferometer testbed (WIIT) at NASA's Goddard Space Flight Center uses a dual-Michelson interferometric technique. The WIIT combines stellar interferometry with Fourier-transform interferometry to produce high-resolution spatial-spectral data over a large field-of-view. This combined technique could be employed on future NASA missions such as the Space Infrared Interferometric Telescope (SPIRIT) and the Sub-millimeter Probe of the Evolution of Cosmic Structure (SPECS). While both SPIRIT and SPECS would operate at far-infrared wavelengths, the WIIT demonstrates the dual-interferometry technique at visible wavelengths. The WIIT will produce hyperspectral image data, so a true hyperspectral object is necessary. A calibrated hyperspectral image projector (CHIP) has been constructed to provide such an object. The CHIP uses Digital Light Processing (DLP) technology to produce customized, spectrally-diverse scenes. CHIP scenes will have approximately 1.6-micron spatial resolution and the capability of . producing arbitrary spectra in the band between 380 nm and 1.6 microns, with approximately 5-nm spectral resolution. Each pixel in the scene can take on a unique spectrum. Spectral calibration is achieved with an onboard fiber-coupled spectrometer. In this paper we describe the operation of the CHIP. Results from the WIIT observations of CHIP scenes will also be presented.

  6. Research on ground-based LWIR hyperspectral imaging remote gas detection

    NASA Astrophysics Data System (ADS)

    Yang, Zhixiong; Yu, Chunchao; Zheng, Weijian; Lei, Zhenggang; Yan, Min; Yuan, Xiaochun; Zhang, Peizhong

    2015-10-01

    The new progress of ground-based long-wave infrared remote sensing is presented, which describes the windowing spatial and temporal modulation Fourier spectroscopy imaging in details. The prototype forms the interference fringes based on the corner-cube of spatial modulation of Michelson interferometer, using cooled long-wave infrared photovoltaic staring FPA (focal plane array) detector. The LWIR hyperspectral imaging is achieved by the process of collection, reorganization, correction, apodization, FFT etc. from data cube. Noise equivalent sensor response (NESR), which is the sensitivity index of CHIPED-1 LWIR hyperspectral imaging prototype, can reach 5.6×10-8W/(cm-1.sr.cm2) at single sampling. Hyperspectral imaging is used in the field of organic gas VOC infrared detection. Relative to wide band infrared imaging, it has some advantages. Such as, it has high sensitivity, the strong anti-interference ability, identify the variety, and so on.

  7. Investigation of NIR hyperspectral imaging for discriminating melamine in milk powder

    NASA Astrophysics Data System (ADS)

    Fu, Xiaping; Kim, Moon S.; Chao, Kuanglin; Qin, Jianwei; Lim, Jongguk; Lee, Hoyoung; Ying, Yibin

    2013-05-01

    Melamine (2,4,6-triamino-1,3,5-triazine) contamination of food has become an urgent and broadly recognized issue for which rapid and accurate identification methods are needed by the food industry. In this study, the feasibility and effectiveness of near-infrared (NIR) hyperspectral imaging was investigated for detecting melamine in milk powder. Hyperspectral NIR images (144 bands spanning from 990 to 1700 nm) were acquired for Petri dishes containing samples of milk powder mixed with melamine at various concentrations (0.02% to 1%). Spectral bands that showed the most significant differences between pure milk and pure melamine were selected, and two-band difference analysis was applied to the spectrum of each pixel in the sample images to identify melamine particles in milk powders. The resultant images effectively allowed visualization of melamine particle distributions in the samples. The study demonstrated that NIR hyperspectral imaging techniques can qualitatively and quantitatively identify melamine adulteration in milk powders.

  8. Methods for correcting morphological-based deficiencies in hyperspectral images of round objects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    NIR images of curved surfaces contain undesirable artifacts that are a consequence of the morphology, or shape of the sample. A software correction was developed to remove the variation in pixel intensity in hyperspectral images of spherical samples generated on a linescan type imaging system. The c...

  9. High-resolution hyperspectral single-pixel imaging system based on compressive sensing

    NASA Astrophysics Data System (ADS)

    Magalha~es, Filipe; Abolbashari, Mehrdad; Araújo, Francisco M.; Correia, Miguel V.; Farahi, Faramarz

    2012-07-01

    For the first time, a high-resolution hyperspectral single-pixel imaging system based on compressive sensing is presented and demonstrated. The system integrates a digital micro-mirror device array to optically compress the image to be acquired and an optical spectrum analyzer to enable high spectral resolution. The system's ability to successfully reconstruct images with 10 pm spectral resolution is proven.

  10. Distributed Compressive Sensing of Hyperspectral Images Using Low Rank and Structure Similarity Property

    NASA Astrophysics Data System (ADS)

    Huang, Bingchao; Xu, Ke; Wan, Jianwei; Liu, Xu

    2015-11-01

    An efficient method and system for distributed compressive sensing of hyperspectral images is presented, which exploit the low rank and structure similarity property of hyperspectral imagery. In this paper, by integrating the respective characteristics of DSC and CS, a distributed compressive sensing framework is proposed to simultaneously capture and compress hyperspectral images. At the encoder, every band image is measured independently, where almost all computation burdens can be shifted to the decoder, resulting in a very low-complexity encoder. It is simple to operate and easy to hardware implementation. At the decoder, each band image is reconstructed by the method of total variation norm minimize. During each band reconstruction, the low rand structure of band images and spectrum structure similarity are used to give birth to the new regularizers. With combining the new regularizers and other regularizer, we can sufficiently exploit the spatial correlation, spectral correlation and spectral structural redundancy in hyperspectral imagery. A numerical optimization algorithm is also proposed to solve the reconstruction model by augmented Lagrangian multiplier method. Experimental results show that this method can effectively improve the reconstruction quality of hyperspectral images.

  11. Detection of Cracks on Tomatoes Using a Hyperspectral Near-Infrared Reflectance Imaging System

    PubMed Central

    Lee, Hoonsoo; Kim, Moon S.; Jeong, Danhee; Delwiche, Stephen R.; Chao, Kuanglin; Cho, Byoung-Kwan

    2014-01-01

    The objective of this study was to evaluate the use of hyperspectral near-infrared (NIR) reflectance imaging techniques for detecting cuticle cracks on tomatoes. A hyperspectral NIR reflectance imaging system that analyzed the spectral region of 1000–1700 nm was used to obtain hyperspectral reflectance images of 224 tomatoes: 112 with and 112 without cracks along the stem-scar region. The hyperspectral images were subjected to partial least square discriminant analysis (PLS-DA) to classify and detect cracks on the tomatoes. Two morphological features, roundness (R) and minimum-maximum distance (D), were calculated from the PLS-DA images to quantify the shape of the stem scar. Linear discriminant analysis (LDA) and a support vector machine (SVM) were then used to classify R and D. The results revealed 94.6% and 96.4% accuracy for classifications made using LDA and SVM, respectively, for tomatoes with and without crack defects. These data suggest that the hyperspectral near-infrared reflectance imaging system, in addition to traditional NIR spectroscopy-based methods, could potentially be used to detect crack defects on tomatoes and perform quality assessments. PMID:25310472

  12. NASA image-based geological expert system development project for hyperspectral image analysis

    NASA Technical Reports Server (NTRS)

    Chiou, W. C., Sr.

    1985-01-01

    The NASA image-based geological expert system was applied to analyze remotely sensed hyperspectral image data. The major objective is for geologists to identify the earth surface mineral properties directly from the airborne and spaceborne imaging spectrometer data. With certain constraints, it is shown that the system can identify correctly different classes of mineral. It has the built-in learning paradigm to enhance the confidence factor of mineral identification. A very powerful natural language system was incorporated as the user-friendly front end, and the concurrent processing efficiency of the frame-based knowledge representation in the hypercube microsupercomputer simulation was tested.

  13. Hyperspectral image classification for mapping agricultural tillage practices

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An efficient classification framework for mapping agricultural tillage practice using hyperspectral remote sensing imagery is proposed, which has the potential to be implemented practically to provide rapid, accurate, and objective surveying data for precision agricultural management and appraisal f...

  14. Determination of pork quality attributes using hyperspectral imaging technique

    NASA Astrophysics Data System (ADS)

    Qiao, Jun; Wang, Ning; Ngadi, M. O.; Gunenc, Aynur

    2005-11-01

    Meat grading has always been a research topic because of large variations among meat products. Many subjective assessment methods with poor repeatability and tedious procedures are still widely used in meat industry. In this study, a hyperspectral-imaging-based technique was developed to achieve fast, accurate, and objective determination of pork quality attributes. The system was able to extract the spectral and spatial characteristics for simultaneous determination of drip loss and pH in pork meat. Two sets of six significant feature wavelengths were selected for predicting the drip loss (590, 645, 721, 752, 803 and 850 nm) and pH (430, 448, 470, 890, 980 and 999 nm). Two feed-forward neural network models were developed. The results showed that the correlation coefficient (r) between the predicted and actual drip loss and pH were 0.71, and 0.58, respectively, by Model 1 and 0.80 for drip loss and 0.67 for pH by Model 2. The color levels of meat samples were also mapped successfully based on a digitalized Meat Color Standard.

  15. Hyperspectral imaging applied to end-of-life concrete recycling

    NASA Astrophysics Data System (ADS)

    Serranti, Silvia; Bonifazi, Giuseppe

    2014-03-01

    In this paper a new technology, based on HyperSpectral Imaging (HSI) sensors, and related detection architectures, is investigated in order to develop suitable and low cost strategies addressed to: i) preliminary detection and characterization of the composition of the structure to dismantle and ii) definition and implementation of innovative smart detection engines for sorting and/or demolition waste flow stream quality control. The proposed sensing architecture is fast, accurate, affordable and it can strongly contribute to bring down the economic threshold above which recycling is cost efficient. Investigations have been carried out utilizing an HSI device working in the range 1000-1700 nm: NIR Spectral Camera™, embedding an ImSpector™ N17E (SPECIM Ltd, Finland). Spectral data analysis was carried out utilizing the PLS_Toolbox (Version 6.5.1, Eigenvector Research, Inc.) running inside Matlab® (Version 7.11.1, The Mathworks, Inc.), applying different chemometric techniques, selected depending on the materials under investigation. The developed procedure allows assessing the characteristics, in terms of materials identification, such as recycled aggregates and related contaminants, as resulting from end-of-life concrete processing. A good classification of the different classes of material was obtained, being the model able to distinguish aggregates from other materials (i.e. glass, plastic, tiles, paper, cardboard, wood, brick, gypsum, etc.).

  16. A hyperspectral imager for high radiometric accuracy Earth climate studies

    NASA Astrophysics Data System (ADS)

    Espejo, Joey; Drake, Ginger; Heuerman, Karl; Kopp, Greg; Lieber, Alex; Smith, Paul; Vermeer, Bill

    2011-10-01

    We demonstrate a visible and near-infrared prototype pushbroom hyperspectral imager for Earth climate studies that is capable of using direct solar viewing for on-orbit cross calibration and degradation tracking. Direct calibration to solar spectral irradiances allow the Earth-viewing instrument to achieve required climate-driven absolute radiometric accuracies of <0.2% (1σ). A solar calibration requires viewing scenes having radiances 105 higher than typical Earth scenes. To facilitate this calibration, the instrument features an attenuation system that uses an optimized combination of different precision aperture sizes, neutral density filters, and variable integration timing for Earth and solar viewing. The optical system consists of a three-mirror anastigmat telescope and an Offner spectrometer. The as-built system has a 12.2° cross track field of view with 3 arcmin spatial resolution and covers a 350-1050 nm spectral range with 10 nm resolution. A polarization compensated configuration using the Offner in an out of plane alignment is demonstrated as a viable approach to minimizing polarization sensitivity. The mechanical design takes advantage of relaxed tolerances in the optical design by using rigid, non-adjustable diamond-turned tabs for optical mount locating surfaces. We show that this approach achieves the required optical performance. A prototype spaceflight unit is also demonstrated to prove the applicability of these solar cross calibration methods to on-orbit environments. This unit is evaluated for optical performance prior to and after GEVS shake, thermal vacuum, and lifecycle tests.

  17. Hyperspectral image classification for mapping agricultural tillage practices

    NASA Astrophysics Data System (ADS)

    Ran, Qiong; Li, Wei; Du, Qian; Yang, Chenghai

    2015-01-01

    An efficient classification framework for mapping agricultural tillage practice using hyperspectral remote sensing imagery is proposed, which has the potential to be implemented practically to provide rapid, accurate, and objective surveying data for precision agricultural management and appraisal from large-scale remote sensing images. It includes a local region filter [i.e., Gaussian low-pass filter (GLF)] to extract spatial-spectral features, a dimensionality reduction process [i.e., local fisher's discriminate analysis (LFDA)], and the traditional k-nearest neighbor (KNN) classifier, and is denoted as GLF-LFDA-KNN. Compared to our previously used local average filter and adaptive weighted filter, the GLF also considers spatial features in a small neighborhood, but it emphasizes the central pixel itself and is data-independent; therefore, it can achieve the balance between classification accuracy and computational complexity. The KNN classifier has a lower computational complexity compared to the traditional support vector machine (SVM). After classification separability is enhanced by the GLF and LFDA, the less powerful KNN can outperform SVM and the overall computational cost remains lower. The proposed framework can also outperform the SVM with composite kernel (SVM-CK) that uses spatial-spectral features.

  18. Hyperspectral imaging based procedures applied to bottom ash characterization

    NASA Astrophysics Data System (ADS)

    Bonifazi, Giuseppe; Serranti, Silvia

    2007-09-01

    Bottom ash from Municipal Solid Waste Incinerators (MSWIs) is mainly land filled or used as material for the foundation of road in European countries. Bottom ash is usually first crushed to below 40 mm and separated magnetically to recover the steel scrap. The remaining material contains predominantly sand, sinters and pieces of stone, glass and ceramics, which could be used as building material if strict technical and environmental requirements are respected. The main problem is the presence of residual organic matter in the ash and the large surface area presented by the fine fraction that creates leaching values, for elements such as copper, that are above the accepted levels for standard building materials. Main aim of the study was to evaluate the possibility offered by hyperspectral imaging to identify organic matter inside the residues in order to develop control/selection strategies to be implemented inside the bottom ash recycling plant. Reflectance spectra of selected bottom ash samples have been acquired in the VIS-NIR field (400- 1000 nm). Results showed as the organic content of the different samples influences the spectral signatures, in particular an inverse correlation between reflectance level and organic matter content was found.

  19. Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles

    PubMed Central

    Seekell, Kevin; Crow, Matthew J.; Marinakos, Stella; Ostrander, Julie; Chilkoti, Ashutosh; Wax, Adam

    2011-01-01

    This work presents simultaneous imaging and detection of three different cell receptors using three types of plasmonic nanoparticles (NPs). The size, shape, and composition-dependent scattering profiles of these NPs allow for a system of multiple distinct molecular markers using a single optical source. With this goal in mind, tags consisting of anti-epidermal growth factor receptor gold nanorods, anti-insulin-like growth factor 1-R silver nanospheres, and human epidermal growth factor receptor 2Ab gold nanospheres were developed to monitor the expression of receptors commonly overexpressed by cancer cells. These labels were chosen because they scatter strongly in distinct spectral windows. A hyperspectral darkfield microspectroscopy system was developed to record the scattering spectra of cells labeled with these molecular tags. Simultaneous monitoring of multiple tags may lead to applications such as profiling of cell line immunophenotype and investigation of receptor signaling pathways. Single, dual, and triple tag experiments were performed to analyze NP tag specificity as well as their interactions. Distinct resonance peaks were observed in these studies, showing the ability to characterize cell lines using conjugated NPs. However, interpreting shifts in these peaks due to changes in a cellular dielectric environment may be complicated by plasmon coupling between NPs bound to proximal receptors and other coupling mechanisms due to the receptors themselves. PMID:22112108

  20. Detecting leafy spurge in native grassland using hyperspectral image analysis

    NASA Astrophysics Data System (ADS)

    Kloppenburg, Catherine

    Leafy spurge (Euphoria esula L.) is a perennial noxious weed that has been encroaches on the native grassland regions of North America resulting in biological and economic impacts. Leafy spurge growth is most prevalent along river banks and in pasture areas. Due to poor accessibility and the cost and labour associated with data collection, estimates of number and size of leafy spurge infestations is poor. Remote sensing has the ability to cover large areas, providing an alternate means to ground surveys and will allow for the capability to create an accurate baseline of infestations. Airborne hyperspectral data were collected over the two test sites selected on the Blood Reserve in Southern Alberta using a combined Airborne Imaging Spectrometer for different Applications (AISA) Eagle and Hawk sensor systems in July, 2010. This study used advanced analysis tools, including spectral mixture analysis, spectral angle mapper and mixture-tuned matched filter techniques to evaluate the ability to detect leafy spurge patches. The results show that patches of leafy spurge with flowering stem density >40 stems m-2 were identified with 85 % accuracy while identification of lower density stems were less accurate (10 - 40 %). The results are promising with respect to quantifying areas of significant leafy spurge infestation and targeting biological control and potential insect release sites.

  1. Estimating index of refraction from polarimetric hyperspectral imaging measurements.

    PubMed

    Martin, Jacob A; Gross, Kevin C

    2016-08-01

    Current material identification techniques rely on estimating reflectivity or emissivity which vary with viewing angle. As off-nadir remote sensing platforms become increasingly prevalent, techniques robust to changing viewing geometries are desired. A technique leveraging polarimetric hyperspectral imaging (P-HSI), to estimate complex index of refraction, N̂(ν̃), an inherent material property, is presented. The imaginary component of N̂(ν̃) is modeled using a small number of "knot" points and interpolation at in-between frequencies ν̃. The real component is derived via the Kramers-Kronig relationship. P-HSI measurements of blackbody radiation scattered off of a smooth quartz window show that N̂(ν̃) can be retrieved to within 0.08 RMS error between 875 cm-1 ≤ ν̃ ≤ 1250 cm-1. P-HSI emission measurements of a heated smooth Pyrex beaker also enable successful N̂(ν̃) estimates, which are also invariant to object temperature. PMID:27505760

  2. Classification of maize kernels using NIR hyperspectral imaging.

    PubMed

    Williams, Paul J; Kucheryavskiy, Sergey

    2016-10-15

    NIR hyperspectral imaging was evaluated to classify maize kernels of three hardness categories: hard, medium and soft. Two approaches, pixel-wise and object-wise, were investigated to group kernels according to hardness. The pixel-wise classification assigned a class to every pixel from individual kernels and did not give acceptable results because of high misclassification. However by using a predefined threshold and classifying entire kernels based on the number of correctly predicted pixels, improved results were achieved (sensitivity and specificity of 0.75 and 0.97). Object-wise classification was performed using two methods for feature extraction - score histograms and mean spectra. The model based on score histograms performed better for hard kernel classification (sensitivity and specificity of 0.93 and 0.97), while that of mean spectra gave better results for medium kernels (sensitivity and specificity of 0.95 and 0.93). Both feature extraction methods can be recommended for classification of maize kernels on production scale. PMID:27173544

  3. Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles

    NASA Astrophysics Data System (ADS)

    Crow, Matthew J.; Seekell, Kevin; Marinakos, Stella; Ostrander, Julie; Chilkoti, Ashutosh; Wax, Adam P.

    2011-03-01

    This work presents simultaneous imaging and detection of three types of cell receptors using three types of plasmonic nanoparticles. The size, shape, and composition-dependent scattering profiles of these particles allow for a system of multiple distinct molecular markers using a single optical source. With this goal in mind, a system of tags consisting of anti-EGFR gold nanorods, anti-IGF1R silver nanospheres, and anti-HER-2 gold nanospheres was developed for monitoring the expression of three commonly overexpressed receptors in cancer cells. These labels were chosen because they each scatter strongly in a distinct spectral window. A hyperspectral dark-field microscope was developed to record the scattering spectra of cells labeled with these molecular tags. The ability to monitor multiple tags simultaneously may lead to applications such as profiling the immunophenotype of cell lines and gaining better knowledge of receptor signaling pathways. Single, dual, and triple tag experiments were performed to analyze the specificity of the nanoparticle tags as well as their effect on one another. While distinct resonance peaks in these studies show the ability to characterize cell lines using conjugated nanoparticles, shifts in these peaks also indicate changes in the cellular dielectric environment which may not be distinct from plasmon coupling between nanoparticles bound to proximal receptors.

  4. Salient Band Selection for Hyperspectral Image Classification via Manifold Ranking.

    PubMed

    Wang, Qi; Lin, Jianzhe; Yuan, Yuan

    2016-06-01

    Saliency detection has been a hot topic in recent years, and many efforts have been devoted in this area. Unfortunately, the results of saliency detection can hardly be utilized in general applications. The primary reason, we think, is unspecific definition of salient objects, which makes that the previously published methods cannot extend to practical applications. To solve this problem, we claim that saliency should be defined in a context and the salient band selection in hyperspectral image (HSI) is introduced as an example. Unfortunately, the traditional salient band selection methods suffer from the problem of inappropriate measurement of band difference. To tackle this problem, we propose to eliminate the drawbacks of traditional salient band selection methods by manifold ranking. It puts the band vectors in the more accurate manifold space and treats the saliency problem from a novel ranking perspective, which is considered to be the main contributions of this paper. To justify the effectiveness of the proposed method, experiments are conducted on three HSIs, and our method is compared with the six existing competitors. Results show that the proposed method is very effective and can achieve the best performance among the competitors. PMID:27008675

  5. Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles

    NASA Astrophysics Data System (ADS)

    Seekell, Kevin; Crow, Matthew J.; Marinakos, Stella; Ostrander, Julie; Chilkoti, Ashutosh; Wax, Adam

    2011-11-01

    This work presents simultaneous imaging and detection of three different cell receptors using three types of plasmonic nanoparticles (NPs). The size, shape, and composition-dependent scattering profiles of these NPs allow for a system of multiple distinct molecular markers using a single optical source. With this goal in mind, tags consisting of anti-epidermal growth factor receptor gold nanorods, anti-insulin-like growth factor 1-R silver nanospheres, and human epidermal growth factor receptor 2Ab gold nanospheres were developed to monitor the expression of receptors commonly overexpressed by cancer cells. These labels were chosen because they scatter strongly in distinct spectral windows. A hyperspectral darkfield microspectroscopy system was developed to record the scattering spectra of cells labeled with these molecular tags. Simultaneous monitoring of multiple tags may lead to applications such as profiling of cell line immunophenotype and investigation of receptor signaling pathways. Single, dual, and triple tag experiments were performed to analyze NP tag specificity as well as their interactions. Distinct resonance peaks were observed in these studies, showing the ability to characterize cell lines using conjugated NPs. However, interpreting shifts in these peaks due to changes in a cellular dielectric environment may be complicated by plasmon coupling between NPs bound to proximal receptors and other coupling mechanisms due to the receptors themselves.

  6. Radiometric calibration and noise estimation of acousto-optic tunable filter hyperspectral imaging systems.

    PubMed

    Katrašnik, Jaka; Pernuš, Franjo; Likar, Boštjan

    2013-05-20

    The accuracy of the radiometric response of acousto-optic tunable filter (AOTF) hyperspectral imaging systems is crucial for obtaining reliable measurements. It is therefore important to know the radiometric response and noise characteristics of the hyperspectral imaging system used. A radiometric model of an AOTF hyperspectral imaging system composed of an imaging sensor radiometric model (CCD, CMOS, and sCMOS) and an AOTF light transmission model is proposed. Using the radiometric model, a method for obtaining the fixed pattern noise (FPN) of the imaging system by displacing and imaging an illuminated reference target is developed. Methods for estimating the temporal noise of the imaging system, using the photon transfer method, and for correcting FPN are also presented. Noise estimation and image restoration methods were tested on an AOTF hyperspectral imaging system. The results indicate that the developed methods can accurately calculate temporal and FPN, and can effectively correct the acquired images. After correction, the signal-to-noise ratio of the acquired images was shown to increase by 26%. PMID:23736239

  7. Hyperspectral imaging with liquid-crystal tunable filter for biological and agricultural assessment

    NASA Astrophysics Data System (ADS)

    Mao, Chengye; Heitschmidt, Jerry

    1999-01-01

    A hyperspectral imaging system has been developed to provide the capability of both airborne and ground/laboratory data acquisitions. The system consists of modular front imaging optics with a liquid crystal tunable filter (LCTF), a CCD video camera, a frame grabber and a portable computer system. The spectral range is form 450 nm to 750 nm with a 10 nm bandpass for each band acquired. The system can capture different spectral images at a rate up to 14 images per second. Hyperspectral imaging with an LCTF provides a new method for hyperspectral image acquisition. The system allows the user to define a wavelength sequence of up to thirty-two spectrums specifically required for individual application, and can quickly switch from the current wavelength to the next during automated image acquisition. Hyperspectral images of crop fields, vegetation, fruits, and meat were successfully captured during laboratory experiments and airborne image acquisition. The constructed spectral image cube not only shows the spatial features of the target, but also reveals the individual pixels with unique spectral signatures. The imaging system with LCTF is, therefore, very useful in biological and agricultural assessment for detecting variations in crop fields, or defects in samples and products.

  8. Detection of gaseous plumes in IR hyperspectral images using hierarchical clustering

    NASA Astrophysics Data System (ADS)

    Hirsch, Eitan; Agassi, Eyal

    2007-09-01

    The emergence of IR hyperspectral sensors in recent years enables their use in remote environmental monitoring of gaseous plumes. IR hyperspectral imaging combines the unique advantages of traditional remote sensing methods such as multispectral imagery and nonimaging Fourier transform infrared spectroscopy, while eliminating their drawbacks. The most significant improvement introduced by hyperspectral technology is the capability of standoff detection and discrimination of effluent gaseous plumes without need for a clear reference background or any other temporal information. We introduce a novel approach for detection and discrimination of gaseous plumes in IR hyperspectral imagery using a divisive hierarchical clustering algorithm. The utility of the suggested detection algorithm is demonstrated on IR hyperspectral images of the release of two atmospheric tracers. The application of the proposed detection method on the experimental data has yielded a correct identification of all the releases without any false alarms. These encouraging results show that the presented approach can be used as a basis for a complete identification algorithm for gaseous pollutants in IR hyperspectral imagery without the need for a clear background.

  9. Hyperspectral imaging fluorescence excitation scanning for detecting colorectal cancer: pilot study

    NASA Astrophysics Data System (ADS)

    Leavesley, Silas J.; Wheeler, Mikayla; Lopez, Carmen; Baker, Thomas; Favreau, Peter F.; Rich, Thomas C.; Rider, Paul F.; Boudreaux, Carole W.

    2016-03-01

    Optical spectroscopy and hyperspectral imaging have shown the theoretical potential to discriminate between cancerous and non-cancerous tissue with high sensitivity and specificity. To date, these techniques have not been able to be effectively translated to endoscope platforms. Hyperspectral imaging of the fluorescence excitation spectrum represents a new technology that may be well-suited for endoscopic implementation. However, the feasibility of detecting differences between normal and cancerous mucosa using fluorescence excitation-scanning hyperspectral imaging has not been evaluated. The objective of this pilot study was to evaluate the changes in the fluorescence excitation spectrum of resected specimen pairs of colorectal adenocarcinoma and normal colorectal mucosa. Patients being treated for colorectal adenocarcinoma were enrolled. Representative adenocarcinoma and normal colonic mucosa specimens were collected from each case. Specimens were flash frozen in liquid nitrogen. Adenocarcinoma was confirmed by histologic evaluation of H&E permanent sections. Hyperspectral image data of the fluorescence excitation of adenocarcinoma and surrounding normal tissue were acquired using a custom microscope configuration previously developed in our lab. Results demonstrated consistent spectral differences between normal and cancerous tissues over the fluorescence excitation spectral range of 390-450 nm. We conclude that fluorescence excitation-scanning hyperspectral imaging may offer an alternative approach for differentiating adenocarcinoma and surrounding normal mucosa of the colon. Future work will focus on expanding the number of specimen pairs analyzed and will utilize fresh tissues where possible, as flash freezing and reconstituting tissues may have altered the autofluorescence properties.

  10. Sparse sampling for fast hyperspectral coherent anti-Stokes Raman scattering imaging.

    PubMed

    Masia, Francesco; Borri, Paola; Langbein, Wolfgang

    2014-02-24

    We demonstrate a method to increase the acquisition speed in coherent anti-Stokes Raman scattering (CARS) hyperspectral imaging while retaining the relevant spectral information. The method first determines the important spectral components of a sample from a hyper-spectral image over a small number of spatial points but a large number of spectral points covering the accessible spectral range and sampling the instrument spectral resolution at the Nyquist limit. From these components we determine a small set of frequencies needed to retrieve the weights of the components with minimum error for a given measurement noise. Hyperspectral images with a large number of spatial points, for example covering a large spatial region, are then measured at this small set of frequencies, and a reconstruction algorithm is applied to generate the full spectral range and resolution. The resulting spectra are suited to retrieve from the CARS intensity the CARS susceptibility which is linear in the concentration, and apply unsupervised quantitative analysis methods such as FSC3. We demonstrate the method on CARS hyperspectral images of human osteosarcoma U2OS cell, with a reduction in the acquisition time by a factor of 25. This method is suited also for other coherent vibrational microscopy techniques such as stimulated Raman scattering, and in general for hyperspectral imaging techniques with sequential spectral acquisition. PMID:24663723

  11. Multivariate curve resolution for the analysis of remotely sensed thermal infrared hyperspectral images.

    SciTech Connect

    Haaland, David Michael; Stork, Christopher Lyle; Keenan, Michael Robert

    2004-07-01

    While hyperspectral imaging systems are increasingly used in remote sensing and offer enhanced scene characterization relative to univariate and multispectral technologies, it has proven difficult in practice to extract all of the useful information from these systems due to overwhelming data volume, confounding atmospheric effects, and the limited a priori knowledge regarding the scene. The need exists for the ability to perform rapid and comprehensive data exploitation of remotely sensed hyperspectral imagery. To address this need, this paper describes the application of a fast and rigorous multivariate curve resolution (MCR) algorithm to remotely sensed thermal infrared hyperspectral images. Employing minimal a priori knowledge, notably non-negativity constraints on the extracted endmember profiles and a constant abundance constraint for the atmospheric upwelling component, it is demonstrated that MCR can successfully compensate thermal infrared hyperspectral images for atmospheric upwelling and, thereby, transmittance effects. We take a semi-synthetic approach to obtaining image data containing gas plumes by adding emission gas signals onto real hyperspectral images. MCR can accurately estimate the relative spectral absorption coefficients and thermal contrast distribution of an ammonia gas plume component added near the minimum detectable quantity.

  12. Real-time lossy compression of hyperspectral images using iterative error analysis on graphics processing units

    NASA Astrophysics Data System (ADS)

    Sánchez, Sergio; Plaza, Antonio

    2012-06-01

    Hyperspectral image compression is an important task in remotely sensed Earth Observation as the dimensionality of this kind of image data is ever increasing. This requires on-board compression in order to optimize the donwlink connection when sending the data to Earth. A successful algorithm to perform lossy compression of remotely sensed hyperspectral data is the iterative error analysis (IEA) algorithm, which applies an iterative process which allows controlling the amount of information loss and compression ratio depending on the number of iterations. This algorithm, which is based on spectral unmixing concepts, can be computationally expensive for hyperspectral images with high dimensionality. In this paper, we develop a new parallel implementation of the IEA algorithm for hyperspectral image compression on graphics processing units (GPUs). The proposed implementation is tested on several different GPUs from NVidia, and is shown to exhibit real-time performance in the analysis of an Airborne Visible Infra-Red Imaging Spectrometer (AVIRIS) data sets collected over different locations. The proposed algorithm and its parallel GPU implementation represent a significant advance towards real-time onboard (lossy) compression of hyperspectral data where the quality of the compression can be also adjusted in real-time.

  13. Miniaturization of high spectral spatial resolution hyperspectral imagers on unmanned aerial systems

    NASA Astrophysics Data System (ADS)

    Hill, Samuel L.; Clemens, Peter

    2015-06-01

    Traditional airborne environmental monitoring has frequently deployed hyperspectral imaging as a leading tool for characterizing and analyzing a scene's critical spectrum-based signatures for applications in agriculture genomics and crop health, vegetation and mineral monitoring, and hazardous material detection. As the acceptance of hyperspectral evaluation grows in the airborne community, there has been a dramatic trend in moving the technology from use on midsize aircraft to Unmanned Aerial Systems (UAS). The use of UAS accomplishes a number of goals including the reduction in cost to run multiple seasonal evaluations over smaller but highly valuable land-areas, the ability to use frequent data collections to make rapid decisions on land management, and the improvement of spatial resolution by flying at lower altitudes (<500 ft.). Despite this trend, there are several key parameters affecting the use of traditional hyperspectral instruments in UAS with payloads less than 10 lbs. where size, weight and power (SWAP) are critical to how high and how far a given UAS can fly. Additionally, on many of the light-weight UAS, users are frequently trying to capture data from one or more instruments to augment the hyperspectral data collection, thus reducing the amount of SWAP available to the hyperspectral instrumentation. The following manuscript will provide an analysis on a newly-developed miniaturized hyperspectral imaging platform, the Nano-Hyperspec®, which provides full hyperspectral resolution and traditional hyperspectral capabilities without sacrificing performance to accommodate the decreasing SWAP of smaller and smaller UAS platforms. The analysis will examine the Nano-Hyperspec flown in several UAS airborne environments and the correlation of the systems data with LiDAR and other GIS datasets.

  14. Spectral-spatial classification using tensor modeling for cancer detection with hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Lu, Guolan; Halig, Luma; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

    2014-03-01

    As an emerging technology, hyperspectral imaging (HSI) combines both the chemical specificity of spectroscopy and the spatial resolution of imaging, which may provide a non-invasive tool for cancer detection and diagnosis. Early detection of malignant lesions could improve both survival and quality of life of cancer patients. In this paper, we introduce a tensor-based computation and modeling framework for the analysis of hyperspectral images to detect head and neck cancer. The proposed classification method can distinguish between malignant tissue and healthy tissue with an average sensitivity of 96.97% and an average specificity of 91.42% in tumor-bearing mice. The hyperspectral imaging and classification technology has been demonstrated in animal models and can have many potential applications in cancer research and management.

  15. Optical hyperspectral imaging in microscopy and spectroscopy - a review of data acquisition.

    PubMed

    Gao, Liang; Smith, R Theodore

    2015-06-01

    Rather than simply acting as a photographic camera capturing two-dimensional (x, y) intensity images or a spectrometer acquiring spectra (λ), a hyperspectral imager measures entire three-dimensional (x, y, λ) datacubes for multivariate analysis, providing structural, molecular, and functional information about biological cells or tissue with unprecedented detail. Such data also gives clinical insights for disease diagnosis and treatment. We summarize the principles underpinning this technology, highlight its practical implementation, and discuss its recent applications at microscopic to macroscopic scales. Datacube acquisition strategies in hyperspectral imaging x, y, spatial coordinates; λ, wavelength. PMID:25186815

  16. Rapid calibrated high-resolution hyperspectral imaging using tunable laser source

    NASA Astrophysics Data System (ADS)

    Nguyen, Lam K.; Margalith, Eli

    2009-05-01

    We present a novel hyperspectral imaging technique based on tunable laser technology. By replacing the broadband source and tunable filters of a typical NIR imaging instrument, several advantages are realized, including: high spectral resolution, highly variable field-of-views, fast scan-rates, high signal-to-noise ratio, and the ability to use optical fiber for efficient and flexible sample illumination. With this technique, high-resolution, calibrated hyperspectral images over the NIR range can be acquired in seconds. The performance of system features will be demonstrated on two example applications: detecting melamine contamination in wheat gluten and separating bovine protein from wheat protein in cattle feed.

  17. Spectral-spatial classification of hyperspectral images with k-means++ partitional clustering

    NASA Astrophysics Data System (ADS)

    Kazanskiy, Nikolay L.; Serafimovich, Pavel G.; Zimichev, Evgeniy A.

    2015-03-01

    We propose and investigate a complex hyperspectral image classification method with regard to the spatial proximity of pixels. Key feature of the method is that it uses common and relatively simple algorithms to attain high accuracy. The method combines the results of pixel-wise support vector machine classification and a set of contours derived from kmeans++ image clustering. To prevent redundant processing of similar data a principal component analysis is used. The method proposed enables the accuracy and speed of hyperspectral image classification to be enhanced.

  18. Theoretical Analysis of the Sensitivity and Speed Improvement of ISIS over a Comparable Traditional Hyperspectral Imager

    SciTech Connect

    Brian R. Stallard; Stephen M. Gentry

    1998-09-01

    The analysis presented herein predicts that, under signal-independent noise limited conditions, an Information-efficient Spectral Imaging Sensor (ISIS) style hyperspectral imaging system design can obtain significant signal-to-noise ratio (SNR) and speed increase relative to a comparable traditional hyperspectral imaging (HSI) instrument. Factors of forty are reasonable for a single vector, and factors of eight are reasonable for a five-vector measurement. These advantages can be traded with other system parameters in an overall sensor system design to allow a variety of applications to be done that otherwise would be impossible within the constraints of the traditional HSI style design.

  19. Hyperspectral imaging for thermal analysis and remote gas sensing in the short wave infrared

    NASA Astrophysics Data System (ADS)

    Pisani, M.; Bianco, P.; Zucco, M.

    2012-07-01

    A novel hyperspectral imaging device based on Fourier transform analysis applied to a low finesse scanning Fabry-Pérot (F-P) interferometer has been demonstrated in the short wave infrared (SWIR) region. The technique allows the realization of a lightweight and compact instrument yet allowing much faster and/or better quality hyperspectral images with respect to classical instruments based on a dispersive means or on a tunable band-pass filter. The potentialities in spectroscopic applications like remote gas sensing are presented as well as accurate thermal imaging capabilities.

  20. Non-destructive hyperspectral imaging of quarantined Mars Returned Samples

    NASA Astrophysics Data System (ADS)

    Simionovici, Alexandre; Viso, Michel; Beck, Pierre; Lemelle, Laurence; Westphal, Andrew; Vincze, Laszlo; Schoonjans, Tom; Fihman, Francois; Chazalnoel, Pascale; Ferroir, Tristan; Solé, Vicente Armando; Tucoulou, R.

    Introduction: In preparation for the upcoming International Mars Sample Return mission (MSR), returning samples containing potential biohazards, we have implemented a hyperspec-tral method of in-situ analysis of grains performed in BSL4 quarantine conditions, by combining several non-destructive imaging diagnostics. This allows sample transportation on optimized experimental setups, while monitoring the sample quarantine conditions. Our hyperspectral methodology was tested during analyses of meteorites [1-2] and cometary and interstellar grains from the recent NASA Stardust mission [3-6]. Synchrotron Radiation protocols: X-ray analysis methods are widely accepted as the least destructive probes of fragile, unique samples. Diffraction, X-ray fluorescence and ab-sorption micro/nano-spectroscopies were performed on chondritic test samples using focused monochromatic beams at the ESRF synchrotron in Grenoble, France. 2D maps of grain com-position down to ppm concentrations and polycrystalline structure have simultaneously been acquired, followed by X-ray absorption performed on elements of Z 26. Ideally, absorption micro-tomography can later be performed in full-beam mode to record the 3D morphology of the grain followed by fluorescence-tomography in focus-beam mode which complements this picture with a 3D elemental image of the grain. Lab-based protocols: Raman and IR-based spectroscopies have been performed in reflection mode for mineralogical imaging of the grains in the laboratory using commercial microscopes. The spatial resolution varied in the 1-10 m range. Laser limited penetration of opaque samples permits only 2D imaging of the few nanometer-thick outer layers of the grains. Mineralogical maps are now routinely acquired using Raman spectroscopy at sub-micron scales through the 3 container walls of the Martian sample holder, followed by IR few-micrometer spot measurements recording C-based and potential aqueous alteration distributions. Sample Holder: A

  1. Research on Ground-Based LWIR Hyperspectral Imaging Remote Gas Detection.

    PubMed

    Zheng, Wei-jian; Lei, Zheng-gang; Yu, Chun-chao; Yang, Zhi-xiong; Wang, Hai-yangi; Fu, Yan-peng; Li, Xun-niu; Liao, Ning-fang; Su, Jun-hong

    2016-02-01

    The new progress of ground-based long-wave infrared remote sensing is presented, which describes the windowing spatial and temporal modulation Fourier spectroscopy imaging in details. The prototype forms the interference fringes based on the corner-cube of spatial modulation of Michelson interferometer, using cooled long-wave infrared photovoltaic staring FPA (focal plane array) detector. The LWIR hyperspectral imaging is achieved by the process of collection, reorganization, correction, apodization, FFT etc. from data cube. Noise equivalent spectral radiance (NESR), which is the sensitivity index of CHIPED-1 LWIR hyperspectral imaging prototype, can reach 5.6 x 10⁻⁸ W · (cm⁻¹ · sr · cm²)⁻¹ at single sampling. The data is the same as commercial temporal modulation hyperspectral imaging spectrometer. It can prove the advantage of this technique. This technique still has space to be improved. For instance, spectral response range of CHIPED-1 LWIR hyperspectral imaging prototype can reach 11. 5 µm by testing the transmission curve of polypropylene film. In this article, choosing the results of outdoor high-rise and diethyl ether gas experiment as an example, the authors research on the detecting method of 2D distribution chemical gas VOC by infrared hyperspectral imaging. There is no observed diethyl ether gas from the infrared spectral slice of the same wave number in complicated background and low concentration. By doing the difference spectrum, the authors can see the space distribution of diethyl ether gas clearly. Hyperspectral imaging is used in the field of organic gas VOC infrared detection. Relative to wide band infrared imaging, it has some advantages. Such as, it has high sensitivity, the strong anti-interference ability, identify the variety, and so on. PMID:27209776

  2. Determination of Basic Density and Moisture Content of Loblolly Pine Wood Disks using a NIR Hyperspectral Imaging System

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The use of near infrared (NIR) hyperspectral imaging for the estimation of basic density (BD) and moisture content (MC) of loblolly pine (Pinus taeda L.) disks is reported. A total of 125 wood disks ranging in age from 13 to 19 years were analysed. Hyperspectral images were collected using an imagin...

  3. HYPERSPECTRAL/MULTISPECTRAL LINE-SCAN IMAGING SYSTEM FOR AUTOMATED POULTRY CARCASS INSPECTION APPLICATIONS FOR FOOD SAFETY

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral/multispectral line-scan imaging system was developed for differentiation of wholesome and systemically diseased chickens. In-plant testing was conducted for chickens on a commercial evisceration line moving at a speed of 70 birds per minute. Hyperspectral image data was acquired fo...

  4. Identification of unknown waste sites using MIVIS hyperspectral images

    SciTech Connect

    Gomarasca, M.A.; Strobelt, S.

    1996-11-01

    This paper presents the results on the individuation of known and unknown (illegal) waste sites using Landsat TM satellite imagery and airborne MIVIS (Multispectral Infrared and Visible Imaging Spectrometer) data for detailed analysis in Italy. Previous results with Landsat TM imagery were partially positive for large waste site identification and negative for small sites. Information acquired by the MIVIS hyperspectral system presents three main characteristics: local scale study, possibility to plan the proper period based on the objectives of the study, high number of spectral bands with high spectral and geometrical resolution. MIVIS airborne shootings were carried out on 7 July 1994 at noon with 4x4 m pixel resolution. The MIVIS 102 bands` sensors can distinguish even objects with similar spectral behavior, thanks to its high spectral resolution. Identification of degraded sites is obtained using traditional spectral and statistical operators (NDVI, Principal Component Analysis, Maximum Likelihood classifier) and innovative combination of filtered band ratios realized to extract specific waste elements (acid slimes or contaminated soils). One of the aims that concerns with this study is the definition of an operative program for the characterization, identification and classification of defined categories of waste disposal sites. The best schedule for the data collection by airborne MIVIS oriented to this target is defined. The planning of the proper flight, based on the waste sites features, is important to optimize this technology. One of the most efficient methods for detecting hidden waste sites is the thermal inertia so two images are necessary: one during low sun load and one with high sun load. The results obtained are operationally useful and winning. This instrument, supported by correct analysis techniques, may offer new interesting prospects in territorial management and environmental monitoring. 5 refs., 5 figs., 1 tab.

  5. Best band selection of hyperspectral remote sensing image based on differential evolution algorithm

    NASA Astrophysics Data System (ADS)

    Cai, Z.; Li, Z.; Jiang, A.; Chen, X.

    2010-12-01

    The hyperspectral remote sensing makes use of spectrum resolution with the nano-scale collecting image data simultaneously in dozens or hundreds of narrow and adjacent spectral bands above the earth's surface. These hyperspectral remote sensors make it possible to derive a continuous spectrum line for each image pixel (or a special sort of material). It can acquire space information, radiated information and spectrum information of images synchronously, so that it has remarkable application value and extensive development prospect in many related fields. However, the hyperspectral remote sensing images' characteristics, such as hundreds of bands, high spectral resolution and large volumes of data, have induced many problems such as high ratio of redundant information, large-scale storage space query, long processing delay, the Hughes phenomenon and so on. The main approach to solve these problems is making dimensional reduction before the classification or visual interpretation with the hyperspectral image data. There are two main methods for dimensional reduction: feature abstraction and bands selection. Although the feature abstraction that can achieve the purpose of dimensional reduction, in the process of feature abstraction or non-linear changes in both linear transformation, it will cause the loss of the physical implication of the original image data and also make it hard to apply hyperspectral images to visual interpretation. In contrast, band selection method outperforms in terms of being more universal for application. The selected bands can not only be used as attributes (features) for classification but also synthesize RGB false color image for visual interpretation. Therefore, band selection of hyperspectral remote sensing images is an important dimensional reduction method. Here, we design a hyperspectral remote sensing image band selection algorithm based on differential evolution algorithm. Differential evolution is an evolutionary method based on

  6. LiCHI - Liquid Crystal Hyperspectral Imager for simultaneous multispectral imaging in aeronomy.

    PubMed

    Goenka, Chhavi; Semeter, Joshua; Noto, John; Baumgardner, Jeffrey; Riccobono, Juanita; Migliozzi, Michael; Dahlgren, Hanna; Marshall, Robert; Kapali, Sudha; Hirsch, Michael; Hampton, Donald; Akbari, Hassanali

    2015-07-13

    A four channel hyperspectral imager using Liquid Crystal Fabry-Perot (LCFP) etalons has been built and tested. This imager is capable of making measurements simultaneously in four wavelength ranges in the visible spectrum. The instrument was designed to make measurements of natural airglow and auroral emissions in the upper atmosphere of the Earth and was installed and tested at the Poker Flat Research Range in Fairbanks, Alaska from February to April 2014. The results demonstrate the capabilities and challenges this instrument presents as a sensor for aeronomical studies. PMID:26191839

  7. Hyperspectral imaging Fourier transform spectrometers for astronomical and remote sensing observations

    NASA Astrophysics Data System (ADS)

    Rafert, J. Bruce; Sellar, R. Glenn; Holbert, Eirik; Blatt, Joel H.; Tyler, David W.; Durham, Susan E.; Newby, Harold D.

    1994-06-01

    The Florida Institue of Technology and the Phillips Laboratory have developed several advanced visible (0.4-0.8 micrometers ) imaging fourier transform spectrometer (IFTS) brassboards, which simultaneously acquire one spatial and one spectral dimension of the hyperspectral image cube. The initial versions of these instruments may be scanned across a scene or configured with a scan mirror to pick up the second spatial dimension of the image cube. The current visible hyperspectral imagers possess a combination of features, including (1) low to moderate spectral resolution for hundreds/thousands of spectral channels, (2) robust design, with no moving parts, (3) detector limited free spectral range, (4) detector-limited spatial and spectral resolution, and (5) field widened operation. The utility of this type of instrument reaches its logical conclusion however, with an instrument designed to acquire all three dimensions of the hyperspectral image cube (both spatial and one spectral) simultaneously. In this paper we present the (1) detailed optical system designs for the brassboard instruments, (2) the current data acquisition system, (3) data reduction and analysis techniques unique to hyperspectral sensor systems which operate with photometric accuracy, and (4) several methods to modify the basic instrument design to allow simultaneous acquistion of the entire hyperspectral image cube. The hyperspectral sensor systems which are being developed and whose utility is being pioneered by Florida Tech and the Phillips Laboratory are applicable to numerous DoD and civil applications including (1) space object identification, (2) radiometrically correct satellite image and spectral signature database observations, (3) simultaneous spactial/spectral observations of booster plumes for strategic and surrogate tactical missile signature identification, and (4) spatial/spectral visible and IR astronomical observations with photometric accuracy.

  8. Handling large datasets of hyperspectral images: reducing data size without loss of useful information.

    PubMed

    Ferrari, Carlotta; Foca, Giorgia; Ulrici, Alessandro

    2013-11-13

    Hyperspectral Imaging (HSI) is gaining increasing interest in the field of analytical chemistry, since this fast and non-destructive technique allows one to easily acquire a large amount of spectral and spatial information on a wide number of samples in very short times. However, the large size of hyperspectral image data often limits the possible uses of this technique, due to the difficulty of evaluating many samples altogether, for example when one needs to consider a representative number of samples for the implementation of on-line applications. In order to solve this problem, we propose a novel chemometric strategy aimed to significantly reduce the dataset size, which allows to analyze in a completely automated way from tens up to hundreds of hyperspectral images altogether, without losing neither spectral nor spatial information. The approach essentially consists in compressing each hyperspectral image into a signal, named hyperspectrogram, which is created by combining several quantities obtained by applying PCA to each single hyperspectral image. Hyperspectrograms can then be used as a compact set of descriptors and subjected to blind analysis techniques. Moreover, a further improvement of both data compression and calibration/classification performances can be achieved by applying proper variable selection methods to the hyperspectrograms. A visual evaluation of the correctness of the choices made by the algorithm can be obtained by representing the selected features back into the original image domain. Likewise, the interpretation of the chemical information underlying the selected regions of the hyperspectrograms related to the loadings is enabled by projecting them in the original spectral domain. Examples of applications of the hyperspectrogram-based approach to hyperspectral images of food samples in the NIR range (1000-1700 nm) and in the vis-NIR range (400-1000 nm), facing a calibration and a defect detection issue respectively, demonstrate the

  9. Detection of mechanical injury on pickling cucumbers using near-infrared hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Ariana, D.; Lu, R.; Guyer, D.

    2005-11-01

    Automated detection of defects on freshly harvested pickling cucumbers will help the pickle industry provide higher quality pickle products and reduce potential economic losses. Research was conducted on using a hyperspectral imaging system for detecting defects on pickling cucumbers caused by mechanical stress. A near-infrared hyperspectral imaging system was used to capture both spatial and spectral information from cucumbers in the spectral region of 900 - 1700 nm. The system consisted of an imaging spectrograph attached to an InGaAs camera with line-light fiber bundles as an illumination source. Cucumber samples were subjected to two forms of mechanical loading, dropping and rolling, to simulate stress caused by mechanical harvesting. Hyperspectral images were acquired from the cucumbers over time periods of 0, 1, 2, 3, and 6 days after mechanical stress. Hyperspectral image processing methods, including principal component analysis and wavelength selection, were developed to separate normal and mechanically injured cucumbers. Results showed that reflectance from normal or non-bruised cucumbers was consistently higher than that from bruised cucumbers. The spectral region between 950 and 1350 nm was found to be most effective for bruise detection. The hyperspectral imaging system detected all mechanically injured cucumbers immediately after they were bruised. The overall detection accuracy was 97% within two hours of bruising and it was lower as time progressed. Lower detection accuracies for the prolonged times after bruising were attributed to the self- healing of the bruised tissue after mechanical injury. This research demonstrated that hyperspectral imaging is useful for detecting mechanical injury on pickling cucumbers.

  10. Accounting for Variance in Hyperspectral Data Coming from Limitations of the Imaging System

    NASA Astrophysics Data System (ADS)

    Shurygin, B.; Shestakova, M.; Nikolenko, A.; Badasen, E.; Strakhov, P.

    2016-06-01

    Over the course of the past few years, a number of methods was developed to incorporate hyperspectral imaging specifics into generic data mining techniques, traditionally used for hyperspectral data processing. Projection pursuit methods embody the largest class of methods empoyed for hyperspectral image data reduction, however, they all have certain drawbacks making them either hard to use or inefficient. It has been shown that hyperspectral image (HSI) statistics tend to display "heavy tails" (Manolakis2003)(Theiler2005), rendering most of the projection pursuit methods hard to use. Taking into consideration the magnitude of described deviations of observed data PDFs from normal distribution, it is apparent that a priori knowledge of variance in data caused by the imaging system is to be employed in order to efficiently classify objects on HSIs (Kerr, 2015), especially in cases of wildly varying SNR. A number of attempts to describe this variance and compensating techniques has been made (Aiazzi2006), however, new data quality standards are not yet set and accounting for the detector response is made under large set of assumptions. Current paper addresses the issue of hyperspectral image classification in the context of different variance sources based on the knowledge of calibration curves (both spectral and radiometric) obtained for each pixel of imaging camera. A camera produced by ZAO NPO Lepton (Russia) was calibrated and used to obtain a test image. A priori known values of SNR and spectral channel cross-correlation were incorporated into calculating test statistics used in dimensionality reduction and feature extraction. Expectation-Maximization classification algorithm modification for non-Gaussian model as described by (Veracini2010) was further employed. The impact of calibration data coarsening by ignoring non-uniformities on false alarm rate was studied. Case study shows both regions of scene-dominated variance and sensor-dominated variance, leading

  11. Spectral-spatial classification for noninvasive cancer detection using hyperspectral imaging

    PubMed Central

    Lu, Guolan; Halig, Luma; Wang, Dongsheng; Qin, Xulei; Chen, Zhuo Georgia; Fei, Baowei

    2014-01-01

    Abstract. Early detection of malignant lesions could improve both survival and quality of life of cancer patients. Hyperspectral imaging (HSI) has emerged as a powerful tool for noninvasive cancer detection and diagnosis, with the advantage of avoiding tissue biopsy and providing diagnostic signatures without the need of a contrast agent in real time. We developed a spectral-spatial classification method to distinguish cancer from normal tissue on hyperspectral images. We acquire hyperspectral reflectance images from 450 to 900 nm with a 2-nm increment from tumor-bearing mice. In our animal experiments, the HSI and classification method achieved a sensitivity of 93.7% and a specificity of 91.3%. The preliminary study demonstrated that HSI has the potential to be applied in vivo for noninvasive detection of tumors. PMID:25277147

  12. Spectral-spatial classification for noninvasive cancer detection using hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Lu, Guolan; Halig, Luma; Wang, Dongsheng; Qin, Xulei; Chen, Zhuo Georgia; Fei, Baowei

    2014-10-01

    Early detection of malignant lesions could improve both survival and quality of life of cancer patients. Hyperspectral imaging (HSI) has emerged as a powerful tool for noninvasive cancer detection and diagnosis, with the advantage of avoiding tissue biopsy and providing diagnostic signatures without the need of a contrast agent in real time. We developed a spectral-spatial classification method to distinguish cancer from normal tissue on hyperspectral images. We acquire hyperspectral reflectance images from 450 to 900 nm with a 2-nm increment from tumor-bearing mice. In our animal experiments, the HSI and classification method achieved a sensitivity of 93.7% and a specificity of 91.3%. The preliminary study demonstrated that HSI has the potential to be applied in vivo for noninvasive detection of tumors.

  13. Along-track calibration of SWIR push-broom hyperspectral imaging system

    NASA Astrophysics Data System (ADS)

    Jemec, Jurij; Pernuš, Franjo; Likar, Boštjan; Bürmen, Miran

    2016-05-01

    Push-broom hyperspectral imaging systems are increasingly used for various medical, agricultural and military purposes. The acquired images contain spectral information in every pixel of the imaged scene collecting additional information about the imaged scene compared to the classical RGB color imaging. Due to the misalignment and imperfections in the optical components comprising the push-broom hyperspectral imaging system, variable spectral and spatial misalignments and blur are present in the acquired images. To capture these distortions, a spatially and spectrally variant response function must be identified at each spatial and spectral position. In this study, we propose a procedure to characterize the variant response function of Short-Wavelength Infrared (SWIR) push-broom hyperspectral imaging systems in the across-track and along-track direction and remove its effect from the acquired images. A custom laser-machined spatial calibration targets are used for the characterization. The spatial and spectral variability of the response function in the across-track and along-track direction is modeled by a parametrized basis function. Finally, the characterization results are used to restore the distorted hyperspectral images in the across-track and along-track direction by a Richardson-Lucy deconvolution-based algorithm. The proposed calibration method in the across-track and along-track direction is thoroughly evaluated on images of targets with well-defined geometric properties. The results suggest that the proposed procedure is well suited for fast and accurate spatial calibration of push-broom hyperspectral imaging systems.

  14. Evaluation of Hyperspectral Imaging and Predictive Modeling to Determine Fertility and Development of Broiler Hatching Eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A hyperspectral imaging system and a predictive modeling technique was evaluated for determining fertility and early embryo development of broiler chicken hatching eggs. Twenty-four broiler-strain eggs were collected (12 fertile, 12 infertile) for each of 8 replicate trials (n=192) and imaged on Da...

  15. ESTIMATING WITHIN-FIELD VARIATIONS IN SOIL PROPERTIES FROM AIRBORNE HYPERSPECTRAL IMAGES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The ability of hyperspectral image (HSI) data to provide estimates of soil electrical conductivity (EC) and soil fertility levels without requiring extensive field data collection was investigated. Bare soil images were acquired using a prism grating pushbroom scanner in April 2000 and May 2001 for ...

  16. A four-dimensional snapshot hyperspectral video-endoscope for bio-imaging applications

    PubMed Central

    Lim, Hoong-Ta; Murukeshan, Vadakke Matham

    2016-01-01

    Hyperspectral imaging has proven significance in bio-imaging applications and it has the ability to capture up to several hundred images of different wavelengths offering relevant spectral signatures. To use hyperspectral imaging for in vivo monitoring and diagnosis of the internal body cavities, a snapshot hyperspectral video-endoscope is required. However, such reported systems provide only about 50 wavelengths. We have developed a four-dimensional snapshot hyperspectral video-endoscope with a spectral range of 400–1000 nm, which can detect 756 wavelengths for imaging, significantly more than such systems. Capturing the three-dimensional datacube sequentially gives the fourth dimension. All these are achieved through a flexible two-dimensional to one-dimensional fiber bundle. The potential of this custom designed and fabricated compact biomedical probe is demonstrated by imaging phantom tissue samples in reflectance and fluorescence imaging modalities. It is envisaged that this novel concept and developed probe will contribute significantly towards diagnostic in vivo biomedical imaging in the near future. PMID:27044607

  17. A four-dimensional snapshot hyperspectral video-endoscope for bio-imaging applications.

    PubMed

    Lim, Hoong-Ta; Murukeshan, Vadakke Matham

    2016-01-01

    Hyperspectral imaging has proven significance in bio-imaging applications and it has the ability to capture up to several hundred images of different wavelengths offering relevant spectral signatures. To use hyperspectral imaging for in vivo monitoring and diagnosis of the internal body cavities, a snapshot hyperspectral video-endoscope is required. However, such reported systems provide only about 50 wavelengths. We have developed a four-dimensional snapshot hyperspectral video-endoscope with a spectral range of 400-1000 nm, which can detect 756 wavelengths for imaging, significantly more than such systems. Capturing the three-dimensional datacube sequentially gives the fourth dimension. All these are achieved through a flexible two-dimensional to one-dimensional fiber bundle. The potential of this custom designed and fabricated compact biomedical probe is demonstrated by imaging phantom tissue samples in reflectance and fluorescence imaging modalities. It is envisaged that this novel concept and developed probe will contribute significantly towards diagnostic in vivo biomedical imaging in the near future. PMID:27044607

  18. Detection of melamine in milk powders based on NIR hyperspectral imaging and spectral similarity analyses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Melamine (2,4,6-triamino-1,3,5-triazine) contamination of food has become an urgent and broadly recognized topic as a result of several food safety scares in the past five years. Hyperspectral imaging techniques that combine the advantages of spectroscopy and imaging have been widely applied for a v...

  19. Line-scan hyperspectral imaging for real-time on-line poultry fecal detection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The preliminary results demonstrated that high speed line-scan hyperspectral imaging system has a potential for real-time online fecal detection during poultry processing. To improve detection accuracy, fully calibrated images both spatially and spectrally were acquired for further processing. In ad...

  20. A comparison of hyperspectral reflectance and fluorescence imaging techniques for detection of contaminants on leafy greens

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ensuring the supply of safe, contaminant free fresh fruit and vegetables is of importance to consumers, suppliers and governments worldwide. In this study, three hyperspectral imaging (HSI) configurations coupled with two multivariate image analysis techniques are compared for detection of fecal con...