Science.gov

Sample records for active safety features

  1. Innovative safety features of the modular HTGR

    SciTech Connect

    Silady, F.A.; Simon, W.A.

    1992-01-01

    The Modular High Temperature Gas-Cooled Reactor (MHTGR) is an advanced reactor concept under development through a cooperative program involving the US Government, the nuclear industry, and the utilities. Near-term development is focused on electricity generation. The top-level safety requirement is that the plant's operation not disturb the normal day-to-day activities of the public. Quantitatively, this requires that the design meet the US Environmental Protection Agency's Protective Action Guides at the site boundary and hence preclude the need for sheltering or evacuation of the public. To meet these stringent safety requirements and at the same time provide a cost competitive design requires the innovative use of the basic high temperature gas-cooled reactor features of ceramic fuel, helium coolant, and a graphite moderator. The specific fuel composition and core size and configuration have been selected to the use the natural characteristics of these materials to develop significantly higher margins of safety. In this document the innovative safety features of the MHTGR are reviewed by examining the safety response to events challenging the functions relied on to retain radionuclides within the coated fuel particles. A broad range of challenges to core heat removal are examined, including a loss of helium pressure of a simultaneous loss of forced cooling of the core. The challenges to control of heat generation consider not only the failure to insert the reactivity control systems but also the withdrawal of control rods. Finally, challenges to control of chemical attack of the ceramic-coated fuel are considered, including catastrophic failure of the steam generator, which allows water ingress, or failure of the pressure vessels, which allows air ingress. The plant's response to these extreme challenges is not dependent on operator action, and the events considered encompass conceivable operator errors.

  2. Innovative safety features of the modular HTGR

    SciTech Connect

    Silady, F.A.; Simon, W.A.

    1992-01-01

    The Modular High Temperature Gas-Cooled Reactor (MHTGR) is an advanced reactor concept under development through a cooperative program involving the US Government, the nuclear industry, and the utilities. Near-term development is focused on electricity generation. The top-level safety requirement is that the plant`s operation not disturb the normal day-to-day activities of the public. Quantitatively, this requires that the design meet the US Environmental Protection Agency`s Protective Action Guides at the site boundary and hence preclude the need for sheltering or evacuation of the public. To meet these stringent safety requirements and at the same time provide a cost competitive design requires the innovative use of the basic high temperature gas-cooled reactor features of ceramic fuel, helium coolant, and a graphite moderator. The specific fuel composition and core size and configuration have been selected to the use the natural characteristics of these materials to develop significantly higher margins of safety. In this document the innovative safety features of the MHTGR are reviewed by examining the safety response to events challenging the functions relied on to retain radionuclides within the coated fuel particles. A broad range of challenges to core heat removal are examined, including a loss of helium pressure of a simultaneous loss of forced cooling of the core. The challenges to control of heat generation consider not only the failure to insert the reactivity control systems but also the withdrawal of control rods. Finally, challenges to control of chemical attack of the ceramic-coated fuel are considered, including catastrophic failure of the steam generator, which allows water ingress, or failure of the pressure vessels, which allows air ingress. The plant`s response to these extreme challenges is not dependent on operator action, and the events considered encompass conceivable operator errors.

  3. Safety features of subcritical fluid fueled systems

    NASA Astrophysics Data System (ADS)

    Bell, Charles R.

    1995-09-01

    Accelerator-driven transmutation technology has been under study at Los Alamos for several years for application to nuclear waste treatment, tritium production, energy generation, and recently, to the disposition of excess weapons plutonium. Studies and evaluations performed to date at Los Alamos have led to a current focus on a fluid-fuel, fission system operating in a neutron source-supported subcritical mode, using molten salt reactor technology and accelerator-driven proton-neutron spallation. In this paper, the safety features and characteristics of such systems are explored from the perspective of the fundamental nuclear safety objectives that any reactor-type system should address. This exploration is qualitative in nature and uses current vintage solid-fueled reactors as a baseline for comparison. Based on the safety perspectives presented, such systems should be capable of meeting the fundamental nuclear safety objectives. In addition, they should be able to provide the safety robustness desired for advanced reactors. However, the manner in which safety objectives and robustness are achieved is very different from that associated with conventional reactors. Also, there are a number of safety design and operational challenges that will have to be addressed for the safety potential of such systems to be credible.

  4. COG - Special Features of Interest to Criticality Safety Practitioners

    SciTech Connect

    Buck, R M; Heinrichs, D P; Krass, A W; Lent, E M

    2010-01-14

    COG is a modern, general-purpose, high fidelity, multi-particle transport code developed at the Lawrence Livermore National Laboratory specifically for use in deep penetration (shielding) and criticality safety calculations. This paper describes some features in COG of special interest to criticality safety practitioners.

  5. Identification and characterization of passive safety system and inherent safety feature building blocks for advanced light-water reactors

    SciTech Connect

    Forsberg, C.W.

    1989-01-01

    Oak Ridge National Laboratory (ORNL) is investigating passive and inherent safety options for Advanced Light-Water Reactors (ALWRs). A major activity in 1989 includes identification and characterization of passive safety system and inherent safety feature building blocks, both existing and proposed, for ALWRs. Preliminary results of this work are reported herein. This activity is part of a larger effort by the US Department of Energy, reactor vendors, utilities, and others in the United States to develop improved LWRs. The Advanced Boiling Water Reactor (ABWR) program and the Advanced Pressurized Water Reactor (APWR) program have as goals improved, commercially available LWRs in the early 1990s. The Advanced Simplified Boiling Water Reactor (ASBWR) program and the AP-600 program are developing more advanced reactors with increased use of passive safety systems. It is planned that these reactors will become commercially available in the mid 1990s. The ORNL program is an exploratory research program for LWRs beyond the year 2000. Desired long-term goals for such reactors include: (1) use of only passive and inherent safety, (2) foolproof against operator errors, (3) malevolence resistance against internal sabotage and external assault and (4) walkaway safety. The acronym ''PRIME'' (Passive safety, Resilient operation, Inherent safety, Malevolence resistance, and Extended (walkaway) safety) is used to summarize these desired characteristics. Existing passive and inherent safety options are discussed in this document.

  6. A child's view: social and physical environmental features differentially predict parent and child perceived neighborhood safety.

    PubMed

    Côté-Lussier, Carolyn; Jackson, Jonathan; Kestens, Yan; Henderson, Melanie; Barnett, Tracie A

    2015-02-01

    Parent and child perceived neighborhood safety predicts child health outcomes such as sleep quality, asthma, physical activity, and psychological distress. Although previous studies identify environmental predictors of parent perceived safety, little is known about predictors of child perceived safety. This study aims to identify the social and physical environmental neighborhood features that predict child and parent perceived neighborhood safety and, simultaneously, to assess the association between child and parent perceptions. Data were from the QUebec Adipose and Lifestyle InvesTigation in Youth (QUALITY) cohort, an ongoing study of Caucasian children (aged 8-10 years) with a parental history of obesity, and their biological parents from Québec, Canada. Measures of social and physical neighborhood features were collected using a spatial data infrastructure and in-person audits. Structural equation modeling was used to test direct and indirect associations between neighborhood features, child and parent perceived safety. Results suggest that among children (N = 494), trees and lighting were positively associated with perceived neighborhood safety, whereas a high proportion of visible minorities was associated with poorer perceived safety. Parents' perceptions of safety were more strongly tied to indicators of disorder and a lack of community involvement, and to traffic. Child perceived safety was partly explained by parent perceived safety, suggesting moderate concordance between perceptions. Although associated with each other, parent and child perceived safety seemed to be determined by distinct environmental features. Though this study focused on determinants of child and parent perceived safety, future research investigating the impact of neighborhood safety on child health should consider both child and parent perspectives.

  7. A Safety Net for New Principals: Ten Features

    ERIC Educational Resources Information Center

    Crippen, Carolyn

    2004-01-01

    The 12 components or features outlined within this article create a broad safety net for new principals and relates directly to the model of school leadership described by Thomas Sergiovanni. Sergiovanni speaks about three components of leadership in school leaders: the educational leader's "heart" (emotions), "head" (thoughts), and "hand"…

  8. Safety Features of Material and Personnel Movement Devices. Module SH-25. Safety and Health.

    ERIC Educational Resources Information Center

    Center for Occupational Research and Development, Inc., Waco, TX.

    This student module on safety features of material and personnel movement devices is one of 50 modules concerned with job safety and health. This module covers safe conditions and operating practices for conveyors, elevators, escalators, moving walks, manlifts, forklifts, and motorized hand trucks. Following the introduction, 10 objectives (each…

  9. Earthquake Safety: Activities for Children.

    ERIC Educational Resources Information Center

    Federal Emergency Management Agency, Washington, DC.

    Lessons on earthquake safety involving planning, preparation, and practice are presented in this booklet for teachers. Included are classroom activities designed for K-6 students, teaching notes, "Learning Links" that summarize interdisciplinary connections, a set of 15 masters for reproducing transparencies, handouts, and worksheets. Part 1 shows…

  10. Fuel Safety Activities in Korea

    SciTech Connect

    Auh, Geun-Sun; Shin, A.D.; Lee, J.S.; Woo, S.W.; Ryu, Y.H.; Kim, Jun-Hwan; Kim, S.K.; Jeong, Y.H.

    2007-07-01

    The current regulatory requirements for fuel performance were based on earlier test data of fresh or low burnup Zircaloy fuels of less than 40 GWD/MTU. Most countries have not changed the current regulatory requirements even if they are actively investigating the high burnup and new cladding alloy effects. Korea agrees with commonly accepted international consensus that although there are technical issues requiring resolutions, these issues do not constitute immediate safety concerns. The high burnup fuel reactor performance experiences of Korea do not show any major problems even if there have been some burnup related fuel failures which are described in the paper. KINS has recommended the industry to have lower fuel failure rates than 1-2 per 50,000 fuel rods. A research project of High Burnup Fuel Safety Tests and Evaluations has started in 2002 under a joint cooperation of KAERI/KNFC/KEPRI and KINS to obtain performance results of high burnup fuel and to develop evaluation technologies of high burnup fuel safety issues. From 1998, KINS has closely monitored and actively participated in international activities such as OECD/NEA CABRI Water Loop Program to reflect on regulatory requirements if needed. KINS will closely monitor the high burnup fuel performances of Korea to strength the regulatory activities if needed. The research activities in Korea including of LOCA and RIA being performed at KAERI with active supports of the industry are summarized in the paper. (authors)

  11. Study of Cost Effective Large Advanced Pressurized Water Reactors that Employ Passive Safety Features

    SciTech Connect

    Winters, J. W.; Corletti, M. M.; Hayashi, Y.

    2003-11-12

    A report of DOE sponsored portions of AP1000 Design Certification effort. On December 16, 1999, The United States Nuclear Regulatory Commission issued Design Certification of the AP600 standard nuclear reactor design. This culminated an 8-year review of the AP600 design, safety analysis and probabilistic risk assessment. The AP600 is a 600 MWe reactor that utilizes passive safety features that, once actuated, depend only on natural forces such as gravity and natural circulation to perform all required safety functions. These passive safety systems result in increased plant safety and have also significantly simplified plant systems and equipment, resulting in simplified plant operation and maintenance. The AP600 meets NRC deterministic safety criteria and probabilistic risk criteria with large margins. A summary comparison of key passive safety system design features is provided in Table 1. These key features are discussed due to their importance in affecting the key thermal-hydraulic phenomenon exhibited by the passive safety systems in critical areas. The scope of some of the design changes to the AP600 is described. These changes are the ones that are important in evaluating the passive plant design features embodied in the certified AP600 standard plant design. These design changes are incorporated into the AP1000 standard plant design that Westinghouse is certifying under 10 CFR Part 52. In conclusion, this report describes the results of the representative design certification activities that were partially supported by the Nuclear Energy Research Initiative. These activities are unique to AP1000, but are representative of research activities that must be driven to conclusion to realize successful licensing of the next generation of nuclear power plants in the United States.

  12. Investigation of road network features and safety performance.

    PubMed

    Wang, Xuesong; Wu, Xingwei; Abdel-Aty, Mohamed; Tremont, Paul J

    2013-07-01

    The analysis of road network designs can provide useful information to transportation planners as they seek to improve the safety of road networks. The objectives of this study were to compare and define the effective road network indices and to analyze the relationship between road network structure and traffic safety at the level of the Traffic Analysis Zone (TAZ). One problem in comparing different road networks is establishing criteria that can be used to scale networks in terms of their structures. Based on data from Orange and Hillsborough Counties in Florida, road network structural properties within TAZs were scaled using 3 indices: Closeness Centrality, Betweenness Centrality, and Meshedness Coefficient. The Meshedness Coefficient performed best in capturing the structural features of the road network. Bayesian Conditional Autoregressive (CAR) models were developed to assess the safety of various network configurations as measured by total crashes, crashes on state roads, and crashes on local roads. The models' results showed that crash frequencies on local roads were closely related to factors within the TAZs (e.g., zonal network structure, TAZ population), while crash frequencies on state roads were closely related to the road and traffic features of state roads. For the safety effects of different networks, the Grid type was associated with the highest frequency of crashes, followed by the Mixed type, the Loops & Lollipops type, and the Sparse type. This study shows that it is possible to develop a quantitative scale for structural properties of a road network, and to use that scale to calculate the relationships between network structural properties and safety. PMID:23584537

  13. Designing Crane Controls with applied Mechanical and Electrical Safety Features

    NASA Technical Reports Server (NTRS)

    Lytle, Bradford P.; Walczak, Thomas A.; Delgado, H. (Technical Monitor)

    2002-01-01

    The use of overhead traveling bridge cranes in many varied applications is common practice. In particular, the use of cranes in the nuclear, military, commercial, aerospace, and other industries can involve safety critical situations. Considerations for Human Injury or Casualty, Loss of Assets, Endangering the Environment, or Economic Reduction must be addressed. Traditionally, in order to achieve additional safety in these applications, mechanical systems have been augmented with a variety of devices. These devices assure that a mechanical component failure shall reduce the risk of a catastrophic loss of the correct and/or safe load carrying capability. ASME NOG-1-1998, (Rules for Construction of Overhead and Gantry Cranes, Top Running Bridge, and Multiple Girder), provides design standards for cranes in safety critical areas. Over and above the minimum safety requirements of todays design standards, users struggle with obtaining a higher degree of reliability through more precise functional specifications while attempting to provide "smart" safety systems. Electrical control systems also may be equipped with protective devices similar to the mechanical design features. Demands for improvement of the cranes "control system" is often recognized, but difficult to quantify for this traditionally "mechanically" oriented market. Finite details for each operation must be examined and understood. As an example, load drift (or small motions) at close tolerances can be unacceptable (and considered critical). To meet these high functional demands encoders and other devices are independently added to control systems to provide motion and velocity feedback to the control drive. This paper will examine the implementation of Programmable Electronic Systems (PES). PES is a term this paper will use to describe any control system utilizing any programmable electronic device such as Programmable Logic Controllers (PLC), or an Adjustable Frequency Drive (AID) 'smart' programmable

  14. Specific features of medicines safety and pharmacovigilance in Africa.

    PubMed

    Isah, Ambrose O; Pal, Shanthi N; Olsson, Sten; Dodoo, Alexander; Bencheikh, Rachida Soulayami

    2012-02-01

    The thalidomide tragedy in the late 1950s and early 1960s served as a wakeup call and raised questions about the safety of medicinal products. The developed countries rose to the challenge putting in place systems to ensure the safety of medicines. However, this was not the case for low-resource settings because of prevailing factors inherent in them. This paper reviews some of these features and the current status of pharmacovigilance in Africa. The health systems in most of the 54 countries of Africa are essentially weak, lacking in basic infrastructure, personnel, equipment and facilities. The recent mass deployment of medicines to address diseases of public health significance in Africa poses additional challenges to the health system with notable safety concerns. Other safety issues of note include substandard and counterfeit medicines, medication errors and quality of medicinal products. The first national pharmacovigilance centres established in Africa with membership of the World Health Organization (WHO) international drug monitoring programme were in Morocco and South Africa in 1992. Of the 104 full member countries in the programme, there are now 24 African countries with a further nine countries as associate members. The pharmacovigilance systems operational in African countries are based essentially on spontaneous reporting facilitated by the introduction of the new tool Vigiflow. The individual case safety reports committed to the WHO global database (Vigibase) attest to the growth of pharmacovigilance in Africa with the number of reports rising from 2695 in 2000 to over 25,000 in 2010. There is need to engage the various identified challenges of the weak pharmacovigilance systems in the African setting and to focus efforts on how to provide resources, infrastructure and expertise. Raising the level of awareness among healthcare providers, developing training curricula for healthcare professionals, provisions for paediatric and geriatric

  15. Nuclear instrumentation, process instrumentation and control, and engineered safety features. Volume nine

    SciTech Connect

    Not Available

    1986-01-01

    Volume nine covers nuclear instrumentation (detection of nuclear radiation, gas-filled detectors, measuring neutron population, BWR/PWR nuclear instrumentation), process instrumentation and control (what is process instrumentation, pressure detectors and transducers, temperature detectors and transducers, level detectors and transducers, flow detectors and transducers, mechanical position detectors and transducers, what are the major processes controlled, BWR and PWR process instrumentation and control), engineered safety features (why are engineered safety features provided, the design basis accident, engineered safety feature operation, PWR engineered safety feature systems, BWR engineered safety feature systems).

  16. Safety in Aquatic Activities. Sports Safety Series. Monograph No. 5.

    ERIC Educational Resources Information Center

    Borozne, Joseph, Ed.; And Others

    The prevention of injuries and control of hazards in aquatic activities is outlined. Discussions include the causes and prevention of aquatic accidents, aquatic safety in the basic instructional program, the design of public swimming facilities, and safety considerations in pool operation and administration. A chapter is devoted to each of the…

  17. Coevolution of active vision and feature selection.

    PubMed

    Floreano, Dario; Kato, Toshifumi; Marocco, Davide; Sauser, Eric

    2004-03-01

    We show that complex visual tasks, such as position- and size-invariant shape recognition and navigation in the environment, can be tackled with simple architectures generated by a coevolutionary process of active vision and feature selection. Behavioral machines equipped with primitive vision systems and direct pathways between visual and motor neurons are evolved while they freely interact with their environments. We describe the application of this methodology in three sets of experiments, namely, shape discrimination, car driving, and robot navigation. We show that these systems develop sensitivity to a number of oriented, retinotopic, visual-feature-oriented edges, corners, height, and a behavioral repertoire to locate, bring, and keep these features in sensitive regions of the vision system, resembling strategies observed in simple insects. PMID:15052484

  18. Coevolution of active vision and feature selection.

    PubMed

    Floreano, Dario; Kato, Toshifumi; Marocco, Davide; Sauser, Eric

    2004-03-01

    We show that complex visual tasks, such as position- and size-invariant shape recognition and navigation in the environment, can be tackled with simple architectures generated by a coevolutionary process of active vision and feature selection. Behavioral machines equipped with primitive vision systems and direct pathways between visual and motor neurons are evolved while they freely interact with their environments. We describe the application of this methodology in three sets of experiments, namely, shape discrimination, car driving, and robot navigation. We show that these systems develop sensitivity to a number of oriented, retinotopic, visual-feature-oriented edges, corners, height, and a behavioral repertoire to locate, bring, and keep these features in sensitive regions of the vision system, resembling strategies observed in simple insects.

  19. Natural and Artificial Playing Fields: Characteristics and Safety Features.

    ERIC Educational Resources Information Center

    Schmidt, Roger C., Ed.; Hoerner, Earl F., Ed.; Milner, Edward M., Ed.; Morehouse, C. A., Ed.

    These papers are on the subjects of playing field standards, surface traction, testing and correlation to actual field experience, and state-of-the-art natural and artificial surfaces. The papers, presented at the Symposium on the Characteristics and Safety of Playing Surfaces (Artificial and Natural) for Field Sports in 1998, cover the…

  20. 75 FR 4610 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-28

    ... Hazardous Materials Safety Administration (PHMSA) published a notice in the Federal Register (74 FR 61403... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration. ACTION: Notice and request for...

  1. Driver face recognition as a security and safety feature

    NASA Astrophysics Data System (ADS)

    Vetter, Volker; Giefing, Gerd-Juergen; Mai, Rudolf; Weisser, Hubert

    1995-09-01

    We present a driver face recognition system for comfortable access control and individual settings of automobiles. The primary goals are the prevention of car thefts and heavy accidents caused by unauthorized use (joy-riders), as well as the increase of safety through optimal settings, e.g. of the mirrors and the seat position. The person sitting on the driver's seat is observed automatically by a small video camera in the dashboard. All he has to do is to behave cooperatively, i.e. to look into the camera. A classification system validates his access. Only after a positive identification, the car can be used and the driver-specific environment (e.g. seat position, mirrors, etc.) may be set up to ensure the driver's comfort and safety. The driver identification system has been integrated in a Volkswagen research car. Recognition results are presented.

  2. An Active Model for Facial Feature Tracking

    NASA Astrophysics Data System (ADS)

    Ahlberg, Jörgen

    2002-12-01

    We present a system for finding and tracking a face and extract global and local animation parameters from a video sequence. The system uses an initial colour processing step for finding a rough estimate of the position, size, and inplane rotation of the face, followed by a refinement step drived by an active model. The latter step refines the pre­vious estimate, and also extracts local animation parame­ters. The system is able to track the face and some facial features in near real-time, and can compress the result to a bitstream compliant to MPEG-4 face and body animation.

  3. An intelligent safety feature for AGV's economic operations: a simulation analysis.

    PubMed

    Nanthavanij, S; Yenradee, P; Techapichetvanich, K

    1995-06-01

    A typical automated guided vehicle (AGV) is equipped with a number of warning and safety devices to prevent injury-causing accidents due to its mishap operations. Standard safety devices, such as an emergency bumper and a non-contact obstacle sensor, sometimes result in uneconomic operations of AGVs since they frequently cause stop-and-go situations. This paper discusses the use of a new intelligent safety feature which not only provides effective warning for the AGV's approach but also helps reduce stop-and-go situations which normally occurred when a sensor detects an obstacle. The proposed feature integrates functional operations of sensors, warning devices, an onboard microprocessor, and the AGV's driving mechanism. Computer simulations of AGV's operations both with and without this intelligent safety feature were also performed and their results are compared.

  4. 77 FR 15453 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-15

    ..., PHMSA published a notice with request for comments in the Federal Register (76 FR 81013). The notice... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  5. 78 FR 55775 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-11

    ... 23, 2013, (78 FR 23972). PHMSA received one comment in response to that notice. PHMSA is publishing... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  6. 76 FR 65778 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-24

    ..., PHMSA published a notice with request for comments in the Federal Register (76 FR 45904). The notice... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  7. 77 FR 58622 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-21

    ... comments on this information collection was published on July 5, 2012 (77 FR 39797). No comments were... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  8. 78 FR 57455 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-18

    ... June 14, 2013, (78 FR 36016). PHMSA received one comment in response to that notice. PHMSA is... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  9. 78 FR 16764 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-18

    ... information collection was published on December 13, 2012, (77 FR 74276) under docket number PHMSA-2012- 0302... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  10. 77 FR 74275 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ... following information collections was published on August 27, 2012, (77 FR 51848) under docket number PHMSA... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  11. Evaluating the safety risk of roadside features for rural two-lane roads using reliability analysis.

    PubMed

    Jalayer, Mohammad; Zhou, Huaguo

    2016-08-01

    The severity of roadway departure crashes mainly depends on the roadside features, including the sideslope, fixed-object density, offset from fixed objects, and shoulder width. Common engineering countermeasures to improve roadside safety include: cross section improvements, hazard removal or modification, and delineation. It is not always feasible to maintain an object-free and smooth roadside clear zone as recommended in design guidelines. Currently, clear zone width and sideslope are used to determine roadside hazard ratings (RHRs) to quantify the roadside safety of rural two-lane roadways on a seven-point pictorial scale. Since these two variables are continuous and can be treated as random, probabilistic analysis can be applied as an alternative method to address existing uncertainties. Specifically, using reliability analysis, it is possible to quantify roadside safety levels by treating the clear zone width and sideslope as two continuous, rather than discrete, variables. The objective of this manuscript is to present a new approach for defining the reliability index for measuring roadside safety on rural two-lane roads. To evaluate the proposed approach, we gathered five years (2009-2013) of Illinois run-off-road (ROR) crash data and identified the roadside features (i.e., clear zone widths and sideslopes) of 4500 300ft roadway segments. Based on the obtained results, we confirm that reliability indices can serve as indicators to gauge safety levels, such that the greater the reliability index value, the lower the ROR crash rate. PMID:27177395

  12. Evaluating the safety risk of roadside features for rural two-lane roads using reliability analysis.

    PubMed

    Jalayer, Mohammad; Zhou, Huaguo

    2016-08-01

    The severity of roadway departure crashes mainly depends on the roadside features, including the sideslope, fixed-object density, offset from fixed objects, and shoulder width. Common engineering countermeasures to improve roadside safety include: cross section improvements, hazard removal or modification, and delineation. It is not always feasible to maintain an object-free and smooth roadside clear zone as recommended in design guidelines. Currently, clear zone width and sideslope are used to determine roadside hazard ratings (RHRs) to quantify the roadside safety of rural two-lane roadways on a seven-point pictorial scale. Since these two variables are continuous and can be treated as random, probabilistic analysis can be applied as an alternative method to address existing uncertainties. Specifically, using reliability analysis, it is possible to quantify roadside safety levels by treating the clear zone width and sideslope as two continuous, rather than discrete, variables. The objective of this manuscript is to present a new approach for defining the reliability index for measuring roadside safety on rural two-lane roads. To evaluate the proposed approach, we gathered five years (2009-2013) of Illinois run-off-road (ROR) crash data and identified the roadside features (i.e., clear zone widths and sideslopes) of 4500 300ft roadway segments. Based on the obtained results, we confirm that reliability indices can serve as indicators to gauge safety levels, such that the greater the reliability index value, the lower the ROR crash rate.

  13. DESIGN SAFETY FEATURES OF THE BNL HIGH-TEMPERATURE COMBUSTION FACILITY

    SciTech Connect

    GINSBERG,T.; CICCARELLI,G.; BOCCIO,J.

    2000-06-11

    The Brookhaven National Laboratory (BNL) High-Temperature Combustion Facility (HTCF) was used to perform hydrogen deflagration and detonation experiments at temperatures to 650 K. Safety features that were designed to ensure safe and reliable operation of the experimental program are described. Deflagration and detonation experiments have been conducted using mixtures of hydrogen, air, and steam. Detonation cell size measurements were made as a function of mixture composition and thermodynamic gas conditions. Deflagration-to-detonation transition experiments were also conducted. Results of the experimental program are presented, and implications with respect to hydrogen safety are discussed.

  14. The prioritization of environment, safety, and health activities

    SciTech Connect

    Otway, H.; Puckett, J.M.; von Winterfeldt, D.

    1991-09-01

    Federal facilities, including the national laboratories, must bring existing operations into compliance with environment, safety, and health (ES H) regulations while restoring sites of past operations to conform with today's more rigorous standards. The need for ES H resources is increasing while overall budgets are decreasing, and the resulting staffing and financial constraints often make it impossible to carry out all necessary activities simultaneously. This stimulated interest in formal methods to prioritize ES H activities. We describe the development of an approach called MAPP (Multi-Attribute Prioritization Process), which features expert judgment, user values, and intensive user participation in the system design process. We present results of its application to the prioritization of 41 ES H activities having a total cost of over $25 million. We conclude that the insights gained from user participation in the design process and the formal prioritization results are probably of comparable value. 19 refs., 3 figs., 9 tabs.

  15. The Foreign Language Feature Film and Language Teaching Activities.

    ERIC Educational Resources Information Center

    Chappell, Martin

    1986-01-01

    Analysis of foreign language films, featuring consideration of film sequence, image and film analysis, and literary adaptation, is an effective teaching activity with foreign language students. An example illustrates film analysis activities in a first-year French class. (CB)

  16. Chapter A9. Safety in Field Activities

    USGS Publications Warehouse

    Lane, Susan L.; Ray, Ronald G.

    1998-01-01

    The National Field Manual for the Collection of Water-Quality Data (National Field Manual) describes protocols (requirements and recommendations) and provides guidelines for U.S. Geological Survey (USGS) personnel who collect data used to assess the quality of the Nation's surface-water and ground-water resources. This chapter of the manual addresses topics related to personal safety to be used in the collection of water-quality data, including: policies and general regulations on field safety; transportation of people and equipment; implementation of surface-water and ground-water activities; procedures for handling chemicals; and information on potentially hazardous environmental conditions, animals, and plants. Each chapter of the National Field Manual is published separately and revised periodically. Newly published and revised chapters will be announced on the USGS Home Page on the World Wide Web under 'New Publications of the U.S. Geological Survey.' The URL for this page is http://pubs.usgs.gov/publications/ index.html.

  17. Active gated imaging for automotive safety applications

    NASA Astrophysics Data System (ADS)

    Grauer, Yoav; Sonn, Ezri

    2015-03-01

    The paper presents the Active Gated Imaging System (AGIS), in relation to the automotive field. AGIS is based on a fast gated-camera equipped with a unique Gated-CMOS sensor, and a pulsed Illuminator, synchronized in the time domain to record images of a certain range of interest which are then processed by computer vision real-time algorithms. In recent years we have learned the system parameters which are most beneficial to night-time driving in terms of; field of view, illumination profile, resolution and processing power. AGIS provides also day-time imaging with additional capabilities, which enhances computer vision safety applications. AGIS provides an excellent candidate for camera-based Advanced Driver Assistance Systems (ADAS) and the path for autonomous driving, in the future, based on its outstanding low/high light-level, harsh weather conditions capabilities and 3D potential growth capabilities.

  18. A risk-based approach to cost-benefit analysis of software safety activities

    SciTech Connect

    Fortier, S.C.; Michael, J.B.

    1993-05-01

    Assumptions about the economics of making a system safe are usually not explicitly stated in industrial and software models of safety-critical systems. These assumptions span a wide spectrum of economic tradeoffs with respect to resources expended to make a system safe. The missing component in these models that is necessary for capturing the effect of economic tradeoffs is risk. A qualitative risk-based software safety model is proposed that combines features of industrial and software systems safety models. The risk-based model provides decision makers with a basis for performing cost-benefit analyses of software safety-related activities.

  19. A risk-based approach to cost-benefit analysis of software safety activities

    SciTech Connect

    Fortier, S.C. ); Michael, J.B. )

    1993-01-01

    Assumptions about the economics of making a system safe are usually not explicitly stated in industrial and software models of safety-critical systems. These assumptions span a wide spectrum of economic tradeoffs with respect to resources expended to make a system safe. The missing component in these models that is necessary for capturing the effect of economic tradeoffs is risk. A qualitative risk-based software safety model is proposed that combines features of industrial and software systems safety models. The risk-based model provides decision makers with a basis for performing cost-benefit analyses of software safety-related activities.

  20. 76 FR 81013 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-27

    ... Statement in the Federal Register published on April 11, 2000 (65 FR 19477) or visit http://www.regulations... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  1. 77 FR 51848 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-27

    ... April 11, 2000 (65 FR 19477) or visit http://www.regulations.gov before submitting any such comments... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  2. 77 FR 74276 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ... Register published on April 11, 2000 (65 FR 19477) or visit http://www.regulations.gov before submitting... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  3. 76 FR 50539 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-15

    ... notice with request for comments in the Federal Register on June 9, 2011 (76 FR 33808) on an information... TRANSPORTATION Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice...

  4. 78 FR 23972 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-23

    ... Privacy Act Statement in the Federal Register published on April 11, 2000, (65 FR 19477) or visit http... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  5. 75 FR 53733 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-01

    ... Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477) or visit http... TRANSPORTATION Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice...

  6. 78 FR 36016 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-14

    ... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477) or visit... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  7. 76 FR 33808 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477) or visit http... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  8. 75 FR 73160 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-29

    ... Register published on April 11, 2000 (65 FR 19477) or visit http://www.regulations.gov before submitting... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration, DOT. ACTION: Notice and request...

  9. 75 FR 40863 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-14

    ... April 11, 2000 (65 FR 19477) or visit http://www.regulations.gov before submitting any such comments... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  10. 75 FR 76077 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-07

    ... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477) or visit... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  11. 76 FR 45904 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477) or visit... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  12. 77 FR 27279 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-09

    ... Privacy Act Statement in the Federal Register published on April 11, 2000, (65 FR 19477) or visit http... TRANSPORTATION Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice...

  13. 77 FR 39797 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... April 11, 2000, (65 FR 19477) or visit http://www.regulations.gov before submitting any such comments... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection Activities AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT. ACTION: Notice and...

  14. 47 CFR 79.109 - Activating accessibility features.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ACCESSIBILITY OF VIDEO PROGRAMMING Apparatus § 79.109 Activating accessibility features. (a) Requirements... video programming transmitted in digital format simultaneously with sound, including apparatus designed to receive or display video programming transmitted in digital format using Internet protocol,...

  15. Extract the Relational Information of Static Features and Motion Features for Human Activities Recognition in Videos

    PubMed Central

    2016-01-01

    Both static features and motion features have shown promising performance in human activities recognition task. However, the information included in these features is insufficient for complex human activities. In this paper, we propose extracting relational information of static features and motion features for human activities recognition. The videos are represented by a classical Bag-of-Word (BoW) model which is useful in many works. To get a compact and discriminative codebook with small dimension, we employ the divisive algorithm based on KL-divergence to reconstruct the codebook. After that, to further capture strong relational information, we construct a bipartite graph to model the relationship between words of different feature set. Then we use a k-way partition to create a new codebook in which similar words are getting together. With this new codebook, videos can be represented by a new BoW vector with strong relational information. Moreover, we propose a method to compute new clusters from the divisive algorithm's projective function. We test our work on the several datasets and obtain very promising results. PMID:27656199

  16. Extract the Relational Information of Static Features and Motion Features for Human Activities Recognition in Videos

    PubMed Central

    2016-01-01

    Both static features and motion features have shown promising performance in human activities recognition task. However, the information included in these features is insufficient for complex human activities. In this paper, we propose extracting relational information of static features and motion features for human activities recognition. The videos are represented by a classical Bag-of-Word (BoW) model which is useful in many works. To get a compact and discriminative codebook with small dimension, we employ the divisive algorithm based on KL-divergence to reconstruct the codebook. After that, to further capture strong relational information, we construct a bipartite graph to model the relationship between words of different feature set. Then we use a k-way partition to create a new codebook in which similar words are getting together. With this new codebook, videos can be represented by a new BoW vector with strong relational information. Moreover, we propose a method to compute new clusters from the divisive algorithm's projective function. We test our work on the several datasets and obtain very promising results.

  17. Convoy Active Safety Technologies Warfighter Experiment I

    NASA Astrophysics Data System (ADS)

    Schoenherr, Edward; Theisen, Bernard L.; Animashaun, Asisat; Davis, James, Jr.; Day, Christopher

    2008-04-01

    The operational ability to project and sustain forces in distant, anti-access and area denial environments poses new challenges for combatant commanders. One of the new challenges is the ability to conduct sustainment operations at operationally feasible times and places on the battlefield. Combatant commanders require a sustainment system that is agile, versatile, and survivable throughout the range of military operations and across the spectrum of conflict. A key component of conducting responsive, operationally feasible sustainment operations is the ability to conduct sustainment convoys. Sustainment convoys are critical to providing combatant commanders the right support, at the right time and place, and in the right quantities, across the full range of military operations. The ability to conduct sustainment convoys in a variety of hostile environments require force protection measures that address the enemy threat and protect the Soldier. One cost effective, technically feasible method of increasing the force protection for sustainment convoys is the use of robotic follower technology and autonomous navigation. The Convoy Active Safety Technologies (CAST) system is a driver assist, convoy autopilot technology aimed to address these issues. Warfigher Experiment I, held at A.P. Hill, VA in the fall of 2007, tested the utility of this vehicle following technology not only in measures of system integrity and performance vs. manual driving, but also the physiological effects on the operators themselves. This paper will detail the Warfigher Experiment's methodology, analysis, results and conclusions.

  18. Lightning Safety and Outdoor Sports Activities

    MedlinePlus

    ... FORECAST Local Graphical Aviation Marine Rivers and Lakes Hurricanes Severe Weather Fire Weather Sun/Moon Long Range ... Safety Campaigns Air Quality Drought Floods Fog Heat Hurricanes Lightning Rip Currents Safe Boating Space Weather Tornadoes, ...

  19. Advantages and safety features using foundation fieldbus-H1 based instrumentation & control for cryo system in accelerators

    NASA Astrophysics Data System (ADS)

    Kaushik, S.; Haneef, K. K. M.; Jayaram, M. N.; Lalsare, D. K.

    2008-05-01

    Large accelerator programme instrumentation and control for monitoring of large no. of parameters for cryogenic/cooling system. The parameters are Cryo Temperature, Vacuum, He Level and He flow etc. The circumference of the accelerator may vary up to several kilometers. Large size accelerators require huge cabling and hardware. The use of foundation fieldbus based Transmitters for measurement and Control valves field positioners for cryo system shall reduce the cabling, hardware, maintenance and enhance data processing and interoperability. Safety is an important requirement for efficient, trouble free and safe operation of any process industry such as cryo used in accelerators. Instrumentation and Control systems can be developed using Foundation Field Bus. The safety features in foundation field bus system can be achieved by use of intrinsic safe devices, fail safe configuration, minimize the hazard by distribution of control function blocks, short circuit preventers. Apart from above features, the significant cable reduction in the fieldbus system reduces the hazard due to electrical cable fire, which is considered one of the major risk in industry. Further the reliability in fieldbus can be improved by hot stand-by redundant power supply, hot stand-by redundant CPU, hot stand-by redundant network capability and use of link active scheduler.

  20. Evaluation of safety effectiveness of multiple cross sectional features on urban arterials.

    PubMed

    Park, Juneyoung; Abdel-Aty, Mohamed

    2016-07-01

    This research evaluates the safety effectiveness of multiple roadway cross-section elements on urban arterials for different crash types and severity levels. In order to consider the nonlinearity of predictors and obtain more reliable estimates, the generalized nonlinear models (GNMs) were developed using 5-years of crash records and roadway characteristics data for urban roadways in Florida. The generalized linear models (GLMs) were also developed to compare model performance. The cross-sectional method was used to develop crash modification factors (CMFs) for various safety treatments. The results from this paper indicated that increasing lane, bike lane, median, and shoulder widths were safety effective to reduce crash frequency. In particular, the CMFs for changes in median and shoulder widths consistently decreased as their widths increased. On the other hand, the safety effects of increasing lane and bike lane widths showed nonlinear variations. It was found that crash rates decrease as the lane width increases until 12ft width and it increases as the lane width exceeds 12ft. The crash rates start to decrease again after 13ft. It was also found that crash rates decreases as the bike lane width increases until 6ft width and it increases as the bike lane width exceeds 6ft. This paper demonstrated that the GNMs clearly captured the nonlinear relationship between crashes and multiple roadway cross-sectional features, which cannot be reflected by the estimated CMFs from the GLMs. Moreover, the GNMs showed better model fitness than GLMs in general. Therefore, in order to estimate more accurate CMFs, the proposed methodology of utilizing the GNMs in the cross-sectional method is recommended over using conventional GLMs when there are nonlinear relationships between the crash rate and roadway characteristics. PMID:27110644

  1. Do active safety-needle devices cause spatter contamination?

    PubMed

    Roff, M; Basu, S; Adisesh, A

    2014-03-01

    Exposure to blood and body fluids is an occupational hazard in healthcare. Although the potential for blood-borne virus transmission through needlestick injury has been widely studied, the risk of this occurring through spatter contamination from safety-needle syringes is not well understood. This report examines this risk from three commonly used safety needles and suggests that this presents a new and significant hazard. Further work should be commissioned to quantify this hazard and determine which type of safety needle would minimize spatter contamination following syringe discharge and safety activation.

  2. Learning Behavior Characterization with Multi-Feature, Hierarchical Activity Sequences

    ERIC Educational Resources Information Center

    Ye, Cheng; Segedy, James R.; Kinnebrew, John S.; Biswas, Gautam

    2015-01-01

    This paper discusses Multi-Feature Hierarchical Sequential Pattern Mining, MFH-SPAM, a novel algorithm that efficiently extracts patterns from students' learning activity sequences. This algorithm extends an existing sequential pattern mining algorithm by dynamically selecting the level of specificity for hierarchically-defined features…

  3. Masked Primes Activate Feature Representations in Reading Aloud

    ERIC Educational Resources Information Center

    Mousikou, Petroula; Roon, Kevin D.; Rastle, Kathleen

    2015-01-01

    Theories of reading aloud are silent about the role of subphonemic/subsegmental representations in translating print to sound. However, there is empirical evidence suggesting that feature representations are activated in speech production and visual word recognition. In the present study, we sought to determine whether masked primes activate…

  4. Unconscious Semantic Activation Depends on Feature-Specific Attention Allocation

    ERIC Educational Resources Information Center

    Spruyt, Adriaan; De Houwer, Jan; Everaert, Tom; Hermans, Dirk

    2012-01-01

    We examined whether semantic activation by subliminally presented stimuli is dependent upon the extent to which participants assign attention to specific semantic stimulus features and stimulus dimensions. Participants pronounced visible target words that were preceded by briefly presented, masked prime words. Both affective and non-affective…

  5. ILO activities in the area of chemical safety.

    PubMed

    Obadia, Isaac

    2003-08-21

    The ILO has been active in the area of safety in the use of chemicals at work since the year of its creation in 1919, including the development of international treaties and other technical instruments, the provision of technical assistance to its member States, and the development of chemical safety information systems. The two key ILO standards in this area are the Conventions on safety in the use of chemicals at work (No. 170, 1990), and the Prevention of Major Industrial Accidents (No. 174, 1993). The ILO Programme on occupational safety, health and environment (Safe Work) is currently responsible for ILO chemical safety activities. In the past two decades, most of ILO work in this area has been carried out within the context of inter-agency collaboration frameworks linking the ILO, WHO, UNEP, FAO, UNIDO, UNITAR, and the OECD, including the International Programme on Chemical Safety (IPCS), the Inter-Organisation Programme for the Sound Management of Chemicals (IOMC), and the Intergovernmental Forum on Chemical Safety (IFCS). Apart from the regular development, updating and dissemination of chemical safety information data bases such as the IPCS International Chemical Cards, the elaboration of a Globally harmonized system for the classification and labelling of Chemicals (GHS) has been the most outstanding achievement of this international collaboration on chemical safety.

  6. ILO activities in the area of chemical safety.

    PubMed

    Obadia, Isaac

    2003-08-21

    The ILO has been active in the area of safety in the use of chemicals at work since the year of its creation in 1919, including the development of international treaties and other technical instruments, the provision of technical assistance to its member States, and the development of chemical safety information systems. The two key ILO standards in this area are the Conventions on safety in the use of chemicals at work (No. 170, 1990), and the Prevention of Major Industrial Accidents (No. 174, 1993). The ILO Programme on occupational safety, health and environment (Safe Work) is currently responsible for ILO chemical safety activities. In the past two decades, most of ILO work in this area has been carried out within the context of inter-agency collaboration frameworks linking the ILO, WHO, UNEP, FAO, UNIDO, UNITAR, and the OECD, including the International Programme on Chemical Safety (IPCS), the Inter-Organisation Programme for the Sound Management of Chemicals (IOMC), and the Intergovernmental Forum on Chemical Safety (IFCS). Apart from the regular development, updating and dissemination of chemical safety information data bases such as the IPCS International Chemical Cards, the elaboration of a Globally harmonized system for the classification and labelling of Chemicals (GHS) has been the most outstanding achievement of this international collaboration on chemical safety. PMID:12909402

  7. The Evaluation of the Safety Benefits of Combined Passive and On-Board Active Safety Applications

    PubMed Central

    Page, Yves; Cuny, Sophie; Zangmeister, Tobias; Kreiss, Jens-Peter; Hermitte, Thierry

    2009-01-01

    One of the objectives of the European TRACE project (TRaffic Accident Causation in Europe, 2006–2008) was to estimate the proportion of injury accidents that could be avoided and/or the proportion of injury accidents where the severity could be mitigated for on-the-market safety applications, if 100 % of the car fleet would be equipped with them. We have selected for evaluation the Electronic Stability Control (ESC) and the Emergency Brake Assist (EBA) applications. As for passive safety systems, recent cars are designed to offer overall safety protection. Car structure, load limiters, front airbags, side airbags, knee airbags, pretensioners, padding and non aggressive structures in the door panel, the dashboard, the windshield, the seats, and the head rest also contribute to applying more protection. The whole safety package is very difficult to evaluate separately, one element independently segmented from the others. We decided to consider evaluating the effectivenessof the whole passive safety package, This package,, for the sake of simplicity, was the number of stars awarded at the Euro NCAP testing. The challenges were to compare the effectiveness of some safety configuration SC I, with the effectiveness of a different safety configuration SC II. A safety configuration is understood as a package of safety functions. Ten comparisons have been carried out such as the evaluation of the safety benefit of a fifth star given that the car has four stars and an EBA. The main outcome of this analysis is that any addition of a passive or active safety function selected in this analysis is producing increased safety benefits. For example, if all cars were five stars fitted with EBA and ESC, instead of four stars without ESC and EBA, injury accidents would be reduced by 47.2% for severe injuries and 69.5% for fatal injuries. PMID:20184838

  8. The evaluation of the safety benefits of combined passive and on-board active safety applications.

    PubMed

    Page, Yves; Cuny, Sophie; Zangmeister, Tobias; Kreiss, Jens-Peter; Hermitte, Thierry

    2009-10-01

    One of the objectives of the European TRACE project (TRaffic Accident Causation in Europe, 2006-2008) was to estimate the proportion of injury accidents that could be avoided and/or the proportion of injury accidents where the severity could be mitigated for on-the-market safety applications, if 100 % of the car fleet would be equipped with them. We have selected for evaluation the Electronic Stability Control (ESC) and the Emergency Brake Assist (EBA) applications. As for passive safety systems, recent cars are designed to offer overall safety protection. Car structure, load limiters, front airbags, side airbags, knee airbags, pretensioners, padding and non aggressive structures in the door panel, the dashboard, the windshield, the seats, and the head rest also contribute to applying more protection. The whole safety package is very difficult to evaluate separately, one element independently segmented from the others. We decided to consider evaluating the effectiveness of the whole passive safety package, This package,, for the sake of simplicity, was the number of stars awarded at the Euro NCAP testing. The challenges were to compare the effectiveness of some safety configuration SC I, with the effectiveness of a different safety configuration SC II. A safety configuration is understood as a package of safety functions. Ten comparisons have been carried out such as the evaluation of the safety benefit of a fifth star given that the car has four stars and an EBA. The main outcome of this analysis is that any addition of a passive or active safety function selected in this analysis is producing increased safety benefits. For example, if all cars were five stars fitted with EBA and ESC, instead of four stars without ESC and EBA, injury accidents would be reduced by 47.2% for severe injuries and 69.5% for fatal injuries. PMID:20184838

  9. Masked primes activate feature representations in reading aloud.

    PubMed

    Mousikou, Petroula; Roon, Kevin D; Rastle, Kathleen

    2015-05-01

    Theories of reading aloud are silent about the role of subphonemic/subsegmental representations in translating print to sound. However, there is empirical evidence suggesting that feature representations are activated in speech production and visual word recognition. In the present study, we sought to determine whether masked primes activate feature representations in reading aloud using a variation of the masked onset priming effect (MOPE). We found that target nonwords (e.g., BAF) were read aloud faster when preceded by masked nonword primes that shared their initial phoneme with the target (e.g., bez), or primes whose initial phoneme shared all features except voicing with the first phoneme of the target (e.g., piz), compared with unrelated primes (e.g., suz). We obtained the same result in 2 experiments that used different participants and prime durations (around 60 ms in Experiment 1 and 50 ms in Experiment 2). The significant masked feature priming effect that was observed in both experiments converges with the empirical evidence in the speech production and visual word recognition domains indicating a functional role for features in reading aloud. Our findings motivate the further development of current theories of reading aloud and have important implications for extant theories of speech production. PMID:25528097

  10. Safety Oversight of Decommissioning Activities at DOE Nuclear Sites

    SciTech Connect

    Zull, Lawrence M.; Yeniscavich, William

    2008-01-15

    The Defense Nuclear Facilities Safety Board (Board) is an independent federal agency established by Congress in 1988 to provide nuclear safety oversight of activities at U.S. Department of Energy (DOE) defense nuclear facilities. The activities under the Board's jurisdiction include the design, construction, startup, operation, and decommissioning of defense nuclear facilities at DOE sites. This paper reviews the Board's safety oversight of decommissioning activities at DOE sites, identifies the safety problems observed, and discusses Board initiatives to improve the safety of decommissioning activities at DOE sites. The decommissioning of former defense nuclear facilities has reduced the risk of radioactive material contamination and exposure to the public and site workers. In general, efforts to perform decommissioning work at DOE defense nuclear sites have been successful, and contractors performing decommissioning work have a good safety record. Decommissioning activities have recently been completed at sites identified for closure, including the Rocky Flats Environmental Technology Site, the Fernald Closure Project, and the Miamisburg Closure Project (the Mound site). The Rocky Flats and Fernald sites, which produced plutonium parts and uranium materials for defense needs (respectively), have been turned into wildlife refuges. The Mound site, which performed R and D activities on nuclear materials, has been converted into an industrial and technology park called the Mound Advanced Technology Center. The DOE Office of Legacy Management is responsible for the long term stewardship of these former EM sites. The Board has reviewed many decommissioning activities, and noted that there are valuable lessons learned that can benefit both DOE and the contractor. As part of its ongoing safety oversight responsibilities, the Board and its staff will continue to review the safety of DOE and contractor decommissioning activities at DOE defense nuclear sites.

  11. Work improvement and occupational safety and health management systems: common features and research needs.

    PubMed

    Kogi, Kazutaka

    2002-04-01

    There is a growing trend in re-orientating occupational health research towards risk management. Such a trend is accelerated by the increasing attention to occupational safety and health management systems. The trend, also seen in many Asian countries, is offering new opportunities for strengthening primary prevention. Useful examples are provided from recent work improvement projects dealing with technology transfer, small workplaces and rural areas. Common features of both these work improvement projects and accepted occupational risk management principles are reviewed based on recent experiences in Asian countries. Such features seem highly relevant in examining the occupational health research strategies. These experiences clearly show that locally adjusted procedures for risk assessment and control must be developed. There are new research needs concerning (a) the effective ways to encourage voluntary control at the workplace; (b) practical methods for local risk assessment; and (c) the types of participatory steps leading to continual improvements in the varying local context. Criteria of action-oriented research that can contribute to more effective risk control in different settings are discussed. Six relevant criteria may be mentioned: (a) adaptive risk management; (b) work/risk relationships; (c) action-oriented risk assessment; (d) use of collective expertise; (e) participation of local people; and (f) mutual learning. It appears crucial to stimulate research into the practical risk control procedures adjusted to the local situation.

  12. The Effect of Line Maintenance Activity on Airline Safety Quality

    NASA Technical Reports Server (NTRS)

    Rhoades, Dawna L.; Reynolds, Rosemarie; Waguespack, Blaise, Jr.; Williams, Michael

    2005-01-01

    One of the arguments against deregulation of the airline industry has been the possibility that financially troubled carriers would be tempted to lower line maintenance spending, thus lowering maintenance quality and decreasing the overall safety of the carrier. Given the financial crisis triggered by the events of 9/11: it appears to be a good time to revisit this issue. This paper examines the quality of airline line maintenance activity and examines the impact of maintenance spending on maintenance quality and overall safety. Findings indicate that increased maintenance spending is associated with increased line maintenance activity and increased overall safety quality for the major U.S. carriers.

  13. Playing It Smart: Safety in Extracurricular Activities

    ERIC Educational Resources Information Center

    Armenta, Tony

    2011-01-01

    An integral part of the school experience for many students is involvement in extracurricular activities such as athletics, cheerleading, band, and others. Likewise, cocurricular activities, such as field trips, provide a chance for students to connect off-campus experiences to the material learned in the classroom. These types of activities,…

  14. Status of Safety& Environmental Activities for Inertial Fusion Energy

    SciTech Connect

    Latkowski, J F; Reyes, S; Cadwallader, L C; Sharpe, J P; Marshall, T D; Merrill, B J; Moore, R L; Petti, D A; Falquina, R; Rodriguez, A; Sanz, J; Cabellos, O

    2002-11-25

    Over the past several years, significant progress has been made in the analysis of safety and environmental (S&E) issues for inertial fusion energy (IFE). Detailed safety assessments have been performed for the baseline power plant concepts, as well as for a conceptual target fabrication facility. Safety analysis results are helping to drive the agenda for experiments. A survey of the S&E characteristics--both radiological and chemical--of candidate target materials has been completed. Accident initiating events have been identified and incorporated into master logic diagrams, which will be essential to the detailed safety analyses that will be needed in the future. Studies of aerosol generation and transport will have important safety implications. A Monte Carlo-based uncertainty analysis procedure has been developed for use in neutron activation calculations. Finally, waste management issues are receiving increased attention and are deserving of further discussion.

  15. Ultra-low-molecular-weight heparins: precise structural features impacting specific anticoagulant activities.

    PubMed

    Lima, Marcelo A; Viskov, Christian; Herman, Frederic; Gray, Angel L; de Farias, Eduardo H C; Cavalheiro, Renan P; Sassaki, Guilherme L; Hoppensteadt, Debra; Fareed, Jawed; Nader, Helena B

    2013-03-01

    Ultra-low-molecular-weight heparins (ULMWHs) with better efficacy and safety ratios are under development; however, there are few structural data available. The main structural features and molecular weight of ULMWHs were studied and compared to enoxaparin. Their monosaccharide composition and average molecular weights were determined and preparations studied by nuclear magnetic resonance spectroscopy, scanning ultraviolet spectroscopy, circular dichroism and gel permeation chromatography. In general, ULMWHs presented higher 3-O-sulphated glucosamine and unsaturated uronic acid residues, the latter being comparable with their higher degree of depolymerisation. The analysis showed that ULMWHs are structurally related to LMWHs; however, their monosaccharide/oligosaccharide compositions and average molecular weights differed considerably explaining their different anticoagulant activities. The results relate structural features to activity, assisting the development of new and improved therapeutic agents, based on depolymerised heparin, for the prophylaxis and treatment of thrombotic disorders.

  16. ON THE 10 mum SILICATE FEATURE IN ACTIVE GALACTIC NUCLEI

    SciTech Connect

    Nikutta, Robert; Elitzur, Moshe; Lacy, Mark E-mail: moshe@pa.uky.ed

    2009-12-20

    The 10 mum silicate feature observed with Spitzer in active galactic nuclei (AGNs) reveals some puzzling behavior. It (1) has been detected in emission in type 2 sources, (2) shows broad, flat-topped emission peaks shifted toward long wavelengths in several type 1 sources, and (3) is not seen in deep absorption in any source observed so far. We solve all three puzzles with our clumpy dust radiative transfer formalism. Addressing (1), we present the spectral energy distribution (SED) of SST1721+6012, the first type 2 quasar observed to show a clear 10 mum silicate feature in emission. Such emission arises in models of the AGN torus easily when its clumpy nature is taken into account. We constructed a large database of clumpy torus models and performed extensive fitting of the observed SED. We find that the cloud radial distribution varies as r {sup -1.5} and the torus contains 2-4 clouds along radial equatorial rays, each with optical depth at visual approx60-80. The source bolometric luminosity is approx3 x 10{sup 12} L{sub sun}. Our modeling suggests that approx<35% of objects with tori sharing these characteristics and geometry would have their central engines obscured. This relatively low obscuration probability can explain the clear appearance of the 10 mum emission feature in SST1721+6012 together with its rarity among other QSO2. Investigating (2), we also fitted the SED of PG1211+143, one of the first type 1 QSOs with a 10 mum silicate feature detected in emission. Together with other similar sources, this QSO appears to display an unusually broadened feature whose peak is shifted toward longer wavelengths. Although this led to suggestions of non-standard dust chemistry in these sources, our analysis fits such SEDs with standard galactic dust; the apparent peak shifts arise from simple radiative transfer effects. Regarding (3), we find additionally that the distribution of silicate feature strengths among clumpy torus models closely resembles the observed

  17. 24 CFR 1006.220 - Crime prevention and safety activities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Crime prevention and safety... URBAN DEVELOPMENT NATIVE HAWAIIAN HOUSING BLOCK GRANT PROGRAM Eligible Activities § 1006.220 Crime... enforcement measures and activities appropriate to protect residents of affordable housing from...

  18. 24 CFR 1006.220 - Crime prevention and safety activities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 4 2014-04-01 2014-04-01 false Crime prevention and safety... URBAN DEVELOPMENT NATIVE HAWAIIAN HOUSING BLOCK GRANT PROGRAM Eligible Activities § 1006.220 Crime... enforcement measures and activities appropriate to protect residents of affordable housing from...

  19. 24 CFR 1006.220 - Crime prevention and safety activities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 4 2011-04-01 2011-04-01 false Crime prevention and safety... URBAN DEVELOPMENT NATIVE HAWAIIAN HOUSING BLOCK GRANT PROGRAM Eligible Activities § 1006.220 Crime... enforcement measures and activities appropriate to protect residents of affordable housing from...

  20. 24 CFR 1006.220 - Crime prevention and safety activities.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 4 2013-04-01 2013-04-01 false Crime prevention and safety... URBAN DEVELOPMENT NATIVE HAWAIIAN HOUSING BLOCK GRANT PROGRAM Eligible Activities § 1006.220 Crime... enforcement measures and activities appropriate to protect residents of affordable housing from...

  1. 24 CFR 1006.220 - Crime prevention and safety activities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Crime prevention and safety... URBAN DEVELOPMENT NATIVE HAWAIIAN HOUSING BLOCK GRANT PROGRAM Eligible Activities § 1006.220 Crime... enforcement measures and activities appropriate to protect residents of affordable housing from...

  2. Features of the Active Evening Plasma Sheet from MMS

    NASA Astrophysics Data System (ADS)

    Moore, T. E.; Chandler, M. O.; Avanov, L. A.; Burch, J. L.; Coffey, V. N.; Ergun, R. E.; Fuselier, S. A.; Gershman, D. J.; Giles, B. L.; Lavraud, B.; MacDonald, E.; Mauk, B.; Mukai, T.; Nakamura, R.; Pollock, C. J.; Russell, C. T.; Saito, Y.; Sauvaud, J. A.; Torbert, R. B.; Yokota, S.

    2015-12-01

    The Magnetospheric Multiscale (MMS) mission, consisting of four identical plasmas and fields observatories, was launched into a 12 RE elliptical equatorial orbit in March 2015 and was in the process of being commissioned through August 2015. During commissioning, the orbit apogee rotated from near midnight through the evening toward the dusk sector and occasionally captured new observations of the plasma sheet, its boundary layers, and the magnetospheric tail lobes. On 22-23 June, an especially active plasma sheet was involved in a major geospace storm that developed a ring current with 200 nT DST. We report on the ion kinetic and flow features of this active plasma sheet, comparing them with familiar observations from earlier missions, as an exercise in validating the MMS observations and assessing their capabilities to provide higher time resolution in multi-point views of thin, fast-moving structures. The observed features include but are not limited to cold lobal wind streams in the lobes, tailward flowing auroral beams and conics, hot earthward field-aligned flows and counter-flows, fast cross-field convection of some flows toward the neutral sheet, and the hot isotropic plasma sheet proper. Relationships between these features, the ionosphere, and the reconnecting magnetotail will be explored and discussed, seeking preliminary conclusions.

  3. Desired features of smartphone applications promoting physical activity.

    PubMed

    Rabin, Carolyn; Bock, Beth

    2011-12-01

    Approximately one-third of adults in the United States are physically inactive. This is a significant public health concern as physical activity (PA) can influence the risk of cardiovascular disease, diabetes, and certain forms of cancer. To minimize these health risks, effective PA interventions must be developed and disseminated to the vast number of individuals who remain sedentary. Smartphone technology presents an exciting opportunity for delivering PA interventions remotely. Although a number of PA applications are currently available for smartphones, these "apps" are not based on established theories of health behavior change and most do not include evidence-based features (e.g., reinforcement and goal setting). Our aim was to collect formative data to develop a smartphone PA app that is empirically and theoretically-based and incorporates user preferences. We recruited 15 sedentary adults to test three currently available PA smartphone apps and provide qualitative and quantitative feedback. Findings indicate that users have a number of specific preferences with regard to PA app features, including that apps provide automatic tracking of PA (e.g., steps taken and calories burned), track progress toward PA goals, and integrate a music feature. Participants also preferred that PA apps be flexible enough to be used with several types of PA, and have well-documented features and user-friendly interfaces (e.g., a one-click main page). When queried by the researcher, most participants endorsed including goal-setting and problem-solving features. These findings provide a blue print for developing a smartphone PA app that incorporates evidence-based components and user preferences. PMID:22010977

  4. Desired features of smartphone applications promoting physical activity.

    PubMed

    Rabin, Carolyn; Bock, Beth

    2011-12-01

    Approximately one-third of adults in the United States are physically inactive. This is a significant public health concern as physical activity (PA) can influence the risk of cardiovascular disease, diabetes, and certain forms of cancer. To minimize these health risks, effective PA interventions must be developed and disseminated to the vast number of individuals who remain sedentary. Smartphone technology presents an exciting opportunity for delivering PA interventions remotely. Although a number of PA applications are currently available for smartphones, these "apps" are not based on established theories of health behavior change and most do not include evidence-based features (e.g., reinforcement and goal setting). Our aim was to collect formative data to develop a smartphone PA app that is empirically and theoretically-based and incorporates user preferences. We recruited 15 sedentary adults to test three currently available PA smartphone apps and provide qualitative and quantitative feedback. Findings indicate that users have a number of specific preferences with regard to PA app features, including that apps provide automatic tracking of PA (e.g., steps taken and calories burned), track progress toward PA goals, and integrate a music feature. Participants also preferred that PA apps be flexible enough to be used with several types of PA, and have well-documented features and user-friendly interfaces (e.g., a one-click main page). When queried by the researcher, most participants endorsed including goal-setting and problem-solving features. These findings provide a blue print for developing a smartphone PA app that incorporates evidence-based components and user preferences.

  5. Design Review Report for formal review of safety class features of exhauster system for rotary mode core sampling

    SciTech Connect

    JANICEK, G.P.

    2000-06-08

    Report documenting Formal Design Review conducted on portable exhausters used to support rotary mode core sampling of Hanford underground radioactive waste tanks with focus on Safety Class design features and control requirements for flammable gas environment operation and air discharge permitting compliance.

  6. Probabilistic cost-benefit analysis of enhanced safety features for strategic nuclear weapons at a representative location

    SciTech Connect

    Stephens, D.R.; Hall, C.H.; Holman, G.S.; Graham, K.F.; Harvey, T.F.; Serduke, F.J.D.

    1993-10-01

    We carried out a demonstration analysis of the value of developing and implementing enhanced safety features for nuclear weapons in the US stockpile. We modified an approach that the Nuclear Regulatory Commission (NRC) developed in response to a congressional directive that NRC assess the ``value-impact`` of regulatory actions for commercial nuclear power plants. Because improving weapon safety shares some basic objectives with NRC regulations, i.e., protecting public health and safety from the effects of accidents involving radioactive materials, we believe the NRC approach to be appropriate for evaluating weapons-safety cost-benefit issues. Impact analysis includes not only direct costs associated with retrofitting the weapon system, but also the expected costs (or economic risks) that are avoided by the action, i.e., the benefits.

  7. Help Yourself! Activities To Promote Safety and Self-Esteem.

    ERIC Educational Resources Information Center

    Ross, Kate

    First in a series of books designed to accompany the compact disk "Help Yourself," this book provides suggestions for classroom activities using the CD songs as a springboard into a curriculum for promoting self-esteem and safety skills among preschool children. Each section begins with sheet music and complete lyrics for each of the 12 songs,…

  8. Taking ownership of safety. What are the active ingredients of safety coaching and how do they impact safety outcomes in critical offshore working environments?

    PubMed

    Krauesslar, Victoria; Avery, Rachel E; Passmore, Jonathan

    2015-01-01

    Safety coaching interventions have become a common feature in the safety critical offshore working environments of the North Sea. Whilst the beneficial impact of coaching as an organizational tool has been evidenced, there remains a question specifically over the use of safety coaching and its impact on behavioural change and producing safe working practices. A series of 24 semi-structured interviews were conducted with three groups of experts in the offshore industry: safety coaches, offshore managers and HSE directors. Using a thematic analysis approach, several significant themes were identified across the three expert groups including connecting with and creating safety ownership in the individual, personal significance and humanisation, ingraining safety and assessing and measuring a safety coach's competence. Results suggest clear utility of safety coaching when applied by safety coaches with appropriate coach training and understanding of safety issues in an offshore environment. The current work has found that the use of safety coaching in the safety critical offshore oil and gas industry is a powerful tool in managing and promoting a culture of safety and care. PMID:26327261

  9. Taking ownership of safety. What are the active ingredients of safety coaching and how do they impact safety outcomes in critical offshore working environments?

    PubMed

    Krauesslar, Victoria; Avery, Rachel E; Passmore, Jonathan

    2015-01-01

    Safety coaching interventions have become a common feature in the safety critical offshore working environments of the North Sea. Whilst the beneficial impact of coaching as an organizational tool has been evidenced, there remains a question specifically over the use of safety coaching and its impact on behavioural change and producing safe working practices. A series of 24 semi-structured interviews were conducted with three groups of experts in the offshore industry: safety coaches, offshore managers and HSE directors. Using a thematic analysis approach, several significant themes were identified across the three expert groups including connecting with and creating safety ownership in the individual, personal significance and humanisation, ingraining safety and assessing and measuring a safety coach's competence. Results suggest clear utility of safety coaching when applied by safety coaches with appropriate coach training and understanding of safety issues in an offshore environment. The current work has found that the use of safety coaching in the safety critical offshore oil and gas industry is a powerful tool in managing and promoting a culture of safety and care.

  10. Neural Features of Processing the Enforcement Phrases Used during Occupational Health and Safety Inspections: An ERP Study

    PubMed Central

    Ma, Qingguo; Shi, Liping; Hu, Linfeng; Liu, Qiang; Yang, Zheng; Wang, Qiuzhen

    2016-01-01

    The appropriate enforcement phrases used during occupational health and safety (OHS) inspection activities is a crucial factor to guarantee the compliance with OHS regulations in enterprises. However, few researchers have empirically investigated the issue of how enforcement phrases are processed. The present study explored the neural features of processing two types of enforcement phrases (severe-and-deterrent vs. mild-and-polite phrases) used during OHS inspections by applying event-related potentials (ERP) method. Electroencephalogram data were recorded while the participants distinguished between severe-and-deterrent phrases and mild-and-polite phrases depicted in written Chinese words. The ERP results showed that severe-and-deterrent phrases elicited significantly augmented P300 amplitude with a central-parietal scalp distribution compared with mild-and-polite phrases, indicating the allocation of more attention resources to and elaborate processing of the severe-and-deterrent phrases. It reveals that humans may consider the severe-and-deterrent phrases as more motivationally significant and elaborately process the severity and deterrence information contained in the enforcement phrases for the adaptive protection. The current study provides an objective and supplementary way to measure the efficiency of different enforcement phrases at neural level, which may help generate appropriate enforcement phrases and improve the performance of OHS inspections. PMID:27807404

  11. Nuclear safety

    NASA Technical Reports Server (NTRS)

    Buden, D.

    1991-01-01

    Topics dealing with nuclear safety are addressed which include the following: general safety requirements; safety design requirements; terrestrial safety; SP-100 Flight System key safety requirements; potential mission accidents and hazards; key safety features; ground operations; launch operations; flight operations; disposal; safety concerns; licensing; the nuclear engine for rocket vehicle application (NERVA) design philosophy; the NERVA flight safety program; and the NERVA safety plan.

  12. 77 FR 22602 - Information Collection Activities: Well Control and Production Safety Training, Submitted for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-16

    ... paperwork requirements in the regulations under Subpart O, ``Well Control and Production Safety Training... Bureau of Safety and Environmental Enforcement (BSEE) Information Collection Activities: Well Control and Production Safety Training, Submitted for Office of Management and Budget (OMB) Review; Comment...

  13. Integration of Active and Passive Safety Technologies--A Method to Study and Estimate Field Capability.

    PubMed

    Hu, Jingwen; Flannagan, Carol A; Bao, Shan; McCoy, Robert W; Siasoco, Kevin M; Barbat, Saeed

    2015-11-01

    The objective of this study is to develop a method that uses a combination of field data analysis, naturalistic driving data analysis, and computational simulations to explore the potential injury reduction capabilities of integrating passive and active safety systems in frontal impact conditions. For the purposes of this study, the active safety system is actually a driver assist (DA) feature that has the potential to reduce delta-V prior to a crash, in frontal or other crash scenarios. A field data analysis was first conducted to estimate the delta-V distribution change based on an assumption of 20% crash avoidance resulting from a pre-crash braking DA feature. Analysis of changes in driver head location during 470 hard braking events in a naturalistic driving study found that drivers' head positions were mostly in the center position before the braking onset, while the percentage of time drivers leaning forward or backward increased significantly after the braking onset. Parametric studies with a total of 4800 MADYMO simulations showed that both delta-V and occupant pre-crash posture had pronounced effects on occupant injury risks and on the optimal restraint designs. By combining the results for the delta-V and head position distribution changes, a weighted average of injury risk reduction of 17% and 48% was predicted by the 50th percentile Anthropomorphic Test Device (ATD) model and human body model, respectively, with the assumption that the restraint system can adapt to the specific delta-V and pre-crash posture. This study demonstrated the potential for further reducing occupant injury risk in frontal crashes by the integration of a passive safety system with a DA feature. Future analyses considering more vehicle models, various crash conditions, and variations of occupant characteristics, such as age, gender, weight, and height, are necessary to further investigate the potential capability of integrating passive and DA or active safety systems. PMID

  14. Software safety analysis activities during software development phases of the Microwave Limb Sounder (MLS)

    NASA Technical Reports Server (NTRS)

    Shaw, Hui-Yin; Sherif, Joseph S.

    2004-01-01

    This paper describes the MLS software safety analysis activities and documents the SSA results. The scope of this software safety effort is consistent with the MLS system safety definition and is concentrated on the software faults and hazards that may have impact on the personnel safety and the environment safety.

  15. Modeling place field activity with hierarchical slow feature analysis

    PubMed Central

    Schönfeld, Fabian; Wiskott, Laurenz

    2015-01-01

    What are the computational laws of hippocampal activity? In this paper we argue for the slowness principle as a fundamental processing paradigm behind hippocampal place cell firing. We present six different studies from the experimental literature, performed with real-life rats, that we replicated in computer simulations. Each of the chosen studies allows rodents to develop stable place fields and then examines a distinct property of the established spatial encoding: adaptation to cue relocation and removal; directional dependent firing in the linear track and open field; and morphing and scaling the environment itself. Simulations are based on a hierarchical Slow Feature Analysis (SFA) network topped by a principal component analysis (ICA) output layer. The slowness principle is shown to account for the main findings of the presented experimental studies. The SFA network generates its responses using raw visual input only, which adds to its biological plausibility but requires experiments performed in light conditions. Future iterations of the model will thus have to incorporate additional information, such as path integration and grid cell activity, in order to be able to also replicate studies that take place during darkness. PMID:26052279

  16. Activation product safety in the ARIES-I reactor design

    SciTech Connect

    Herring, J.S. ); Sze, D.K. ); Wong, C.; Cheng, E.T. ); Grotz, S.P. )

    1990-01-01

    The ARIES design effort has sought to maximize the environmental and safety advantages of fusion through careful selection of materials and careful design. Three goals are that the reactor achieve inherent or passive safety, that no public evacuation plan be necessary and that the waste be disposable as 10CFR61 Class C waste. The ARIES-I reactor consists of a SiC composite structure for the first wall and blanket, cooled by 10 MPa He. The breeder is Li{sub 2}ZrO{sub 3}, although Li{sub 2}O and Li{sub 4}SiO{sub 4} were also considered. The divertor consists of SiC composite tubes coated with 2 mm of tungsten. Due to the minimal afterheat of this blanket design, LOCA calculations indicate maximum temperatures will not cause damage if the plasma is promptly extinguished. Two primary safety issues are the zirconium in the breeder and tungsten on the divertor. Li{sub 2}ZrO{sub 3} was chosen because of its demonstrated high-temperature stability. The other breeders have lower afterheat and activation. Use of zirconium in the breeder will necessitate isotopic tailoring to remove {sup 90}Zr and {sup 94}Zr. The 5.8 tonnes of W on the divertor would also have to be tailored to remove {sup 186}W and/or to concentrate {sup 183}W. Thus the ARIES-I design achieves the passive safety and low-level waste disposal criteria with respect to activation products. Development of low activation materials to replace zirconium and tungsten is needed to avoid requiring an evacuation plan.

  17. Instructional games and activities for criticality safety training

    SciTech Connect

    Bullard, B.; McBride, J. )

    1993-01-01

    During the past several years, the Training and Management Systems Division (TMSD) staff of Oak Ridge Institute for Science and Education (ORISE) has designed and developed nuclear criticality safety (NCS) training programs that focus on high trainee involvement through the use of instructional games and activities. This paper discusses the instructional game, initial considerations for developing games, advantages and limitations of games, and how games may be used in developing and implementing NCS training. It also provides examples of the various instructional games and activities used in separate courses designed for Martin Marietta Energy Systems (MMES's) supervisors and U.S. Nuclear Regulatory Commission (NRC) fuel facility inspectors.

  18. Features, events, processes, and safety factor analysis applied to a near-surface low-level radioactive waste disposal facility

    SciTech Connect

    Stephens, M.E.; Dolinar, G.M.; Lange, B.A.

    1995-12-31

    An analysis of features, events, processes (FEPs) and other safety factors was applied to AECL`s proposed IRUS (Intrusion Resistant Underground Structure) near-surface LLRW disposal facility. The FEP analysis process which had been developed for and applied to high-level and transuranic disposal concepts was adapted for application to a low-level facility for which significant efforts in developing a safety case had already been made. The starting point for this process was a series of meetings of the project team to identify and briefly describe FEPs or safety factors which they thought should be considered. At this early stage participants were specifically asked not to screen ideas. This initial list was supplemented by selecting FEPs documented in other programs and comments received from an initial regulatory review. The entire list was then sorted by topic and common issues were grouped, and issues were classified in three priority categories and assigned to individuals for resolution. In this paper, the issue identification and resolution process will be described, from the initial description of an issue to its resolution and inclusion in the various levels of the safety case documentation.

  19. Handling and safety enhancement of race cars using active aerodynamic systems

    NASA Astrophysics Data System (ADS)

    Diba, Fereydoon; Barari, Ahmad; Esmailzadeh, Ebrahim

    2014-09-01

    A methodology is presented in this work that employs the active inverted wings to enhance the road holding by increasing the downward force on the tyres. In the proposed active system, the angles of attack of the vehicle's wings are adjusted by using a real-time controller to increase the road holding and hence improve the vehicle handling. The handling of the race car and safety of the driver are two important concerns in the design of race cars. The handling of a vehicle depends on the dynamic capabilities of the vehicle and also the pneumatic tyres' limitations. The vehicle side-slip angle, as a measure of the vehicle dynamic safety, should be narrowed into an acceptable range. This paper demonstrates that active inverted wings can provide noteworthy dynamic capabilities and enhance the safety features of race cars. Detailed analytical study and formulations of the race car nonlinear model with the airfoils are presented. Computer simulations are carried out to evaluate the performance of the proposed active aerodynamic system.

  20. Experimental and design experience with passive safety features of liquid metal reactors

    SciTech Connect

    Lucoff, D.M.; Waltar, A.E.; Sackett, J.I.; Salvatores, M.; Aizawa, K.

    1992-10-01

    Liquid metal cooled reactors (LMRs) have already been demonstrated to be robust machines. Many reactor designers now believe that it is possible to include in this technology sufficient passive safety that LMRs would be able to survive loss of flow, loss of heat sink, and transient overpower events, even if the plant protective system fails completely and do so without damage to the core. Early whole-core testing in Rapsodie, EBR-II. and FFTF indicate such designs may be possible. The operational safety testing program in EBR-II is demonstrating benign response of the reactor to a full range of controls failures. But additional testing is needed if transient core structural response under major accident conditions is to be properly understood. The proposed international Phase IIB passive safety tests in FFTF, being designed with a particular emphasis on providing, data to understand core bowing extremes, and further tests planned in EBR-II with processed IFR fuel should provide a substantial and unique database for validating the computer codes being used to simulate postulated accident conditions.

  1. Experimental and design experience with passive safety features of liquid metal reactors

    SciTech Connect

    Lucoff, D.M.; Waltar, A.E. ); Sackett, J.I. ); Aizawa, K. )

    1992-07-01

    Liquid metal cooled reactors (LMRs) have already been demonstrated to be robust machines. Many reactor designers now believe that it is possible to include in this technology sufficient passive safety that LMRs would be able to survive loss of flow, loss of heat sink, and transient overpower events, even if the plant protective system fails completely--and do so without damage to the core. Early whole-core testing in Rapsodie, EBR-II, and FFTF indicate such designs may be possible. The operational safety testing program in EBR-II is demonstrating benign response of the reactor to a full range on controls failures. But additional testing is needed if transient core structural response under major accident conditions is to be properly understood. The proposed international Phase IIB passive safety tests in FFTF, being designed with a particular emphasis on providing data to understand core bowing extremes, and further tests planned in EBR-II with processed IFR fuel should provide a substantial and unique database for validating the computer codes being used to simulate postulated accident conditions.

  2. Autonomous safety and reliability features of the K-1 avionics system

    NASA Astrophysics Data System (ADS)

    Mueller, George E.; Kohrs, Dick; Bailey, Richard; Lai, Gary

    2004-03-01

    Kistler Aerospace Corporation is developing the K-1, a fully reusable, two-stage-to-orbit launch vehicle. Both stages return to the launch site using parachutes and airbags. Initial flight operations will occur from Woomera, Australia. K-1 guidance is performed autonomously. Each stage of the K-1 employs a triplex, fault tolerant avionics architecture, including three fault tolerant computers and three radiation hardened Embedded GPS/INS units with a hardware voter. The K-1 has an Integrated Vehicle Health Management (IVHM) system on each stage residing in the three vehicle computers based on similar systems in commercial aircraft. During first-stage ascent, the IVHM system performs an Instantaneous Impact Prediction (IIP) calculation 25 times per second, initiating an abort in the event the vehicle is outside a predetermined safety corridor for at least 3 consecutive calculations. In this event, commands are issued to terminate thrust, separate the stages, dump all propellant in the first-stage, and initiate a normal landing sequence. The second-stage flight computer calculates its ability to reach orbit along its state vector, initiating an abort sequence similar to the first stage if it cannot. On a nominal mission, following separation, the second-stage also performs calculations to assure its impact point is within a safety corridor. The K-1's guidance and control design is being tested through simulation with hardware-in-the-loop at Draper Laboratory. Kistler's verification strategy assures reliable and safe operation of the K-1.

  3. A review of the safety features of 6M packagings for DOE programs

    SciTech Connect

    Not Available

    1988-12-01

    This report, prepared by a US Department of Energy (DOE) Task Force and organized for clarity into two-page modules, argues that the US Department of Transportation (DOT) Specification-6M packagings (hereafter referred to as 6M packaging, or simply 6M) merit continued DOE use and, if necessary, DOE certification. This report is designed to address the specific requirements of a Safety Analysis Report for Packaging (SARP). While not a SARP, this report constitutes a compilation of all available documentation on 6M packagings. The authors individually, and the Task Force collectively, believe their investigation provides justification for the continued use of 6M packagings because they meet criteria for quality assurance and for safety under normal and accident conditions as defined by the US Nuclear Regulatory Commission (NRC) regulations. This report may be used by DOE managers to assist in deliberations on future requirements for 6M packagings as they are required to support DOE programs. For the purpose of ready evaluation, this report includes categorical topics found in Nuclear Regulatory Guide 7.9, the topical guideline for SARPs. The format, however, will (it is hoped) pleasantly surprise customary reader expectations. For, while maintaining categorical headings and subheadings found in SARPs as a skeleton, the Task Force chose to adopt the document design principles developed by Hughes Aircraft in the 1960s, ''Sequential Thematic Organization of Publications'' (STOP). 37 figs.

  4. Preliminary Safety Analysis of the Gorleben Site: Safety Concept and Application to Scenario Development Based on a Site-Specific Features, Events and Processes (FEP) Database - 13304

    SciTech Connect

    Moenig, Joerg; Beuth, Thomas; Wolf, Jens; Lommerzheim, Andre; Mrugalla, Sabine

    2013-07-01

    Based upon the German safety criteria, released in 2010 by the Federal Ministry of the Environment (BMU), a safety concept and a safety assessment concept for the disposal of heat-generating high-level waste have both been developed in the framework of the preliminary safety case for the Gorleben site (Project VSG). The main objective of the disposal is to contain the radioactive waste inside a defined rock zone, which is called containment-providing rock zone. The radionuclides shall remain essentially at the emplacement site, and at the most, a small defined quantity of material shall be able to leave this rock zone. This shall be accomplished by the geological barrier and a technical barrier system, which is required to seal the inevitable penetration of the geological barrier by the construction of the mine. The safe containment has to be demonstrated for probable and less probable evolutions of the site, while evolutions with very low probability (less than 1 % over the demonstration period of 1 million years) need not to be considered. Owing to the uncertainty in predicting the real evolution of the site, plausible scenarios have been derived in a systematic manner. Therefore, a comprehensive site-specific features, events and processes (FEP) data base for the Gorleben site has been developed. The safety concept was directly taken into account, e.g. by identification of FEP with direct influence on the barriers that provide the containment. No effort was spared to identify the interactions of the FEP, their probabilities of occurrence, and their characteristics (values). The information stored in the data base provided the basis for the development of scenarios. The scenario development methodology is based on FEP related to an impairment of the functionality of a subset of barriers, called initial barriers. By taking these FEP into account in their probable characteristics the reference scenario is derived. Thus, the reference scenario describes a

  5. Latency features of SafetyNet ground systems architecture for the National Polar-orbiting Operational Environmental Satellite System (NPOESS)

    NASA Astrophysics Data System (ADS)

    Duda, James L.; Mulligan, Joseph; Valenti, James; Wenkel, Michael

    2005-01-01

    A key feature of the National Polar-orbiting Operational Environmental Satellite System (NPOESS) is the Northrop Grumman Space Technology patent-pending innovative data routing and retrieval architecture called SafetyNetTM. The SafetyNetTM ground system architecture for the National Polar-orbiting Operational Environmental Satellite System (NPOESS), combined with the Interface Data Processing Segment (IDPS), will together provide low data latency and high data availability to its customers. The NPOESS will cut the time between observation and delivery by a factor of four when compared with today's space-based weather systems, the Defense Meteorological Satellite Program (DMSP) and NOAA's Polar-orbiting Operational Environmental Satellites (POES). SafetyNetTM will be a key element of the NPOESS architecture, delivering near real-time data over commercial telecommunications networks. Scattered around the globe, the 15 unmanned ground receptors are linked by fiber-optic systems to four central data processing centers in the U. S. known as Weather Centrals. The National Environmental Satellite, Data and Information Service; Air Force Weather Agency; Fleet Numerical Meteorology and Oceanography Center, and the Naval Oceanographic Office operate the Centrals. In addition, this ground system architecture will have unused capacity attendant with an infrastructure that can accommodate additional users.

  6. Gaseous core nuclear-driven engines featuring a self-shutoff mechanism to provide nuclear safety

    SciTech Connect

    Heidrich, J.; Pettibone, J.; Chow, Tze-Show; Condit, R.; Zimmerman, G.

    1991-11-01

    Nuclear driven engines are described that could be run in either pulsed or steady state modes. In the pulsed mode nuclear energy is released by fissioning of uranium or plutonium in a supercritical assembly of fuel and working gas. In a steady state mode a fuel-gas mixture is injected into a magnetic nozzle where it is compressed into a critical state and produces energy. Engine performance is modeled using a code that calculates hydrodynamics, fission energy production, and neutron transport self-consistently. Results are given demonstrating a large negative temperature coefficient that produces self-shutoff or control of energy production. Reduced fission product inventory and the self-shutoff provide inherent nuclear safety. It is expected that nuclear engine reactor units could be scaled up from about 100 MW{sub e}.

  7. Generic Safety Issue (GSI) 171 -- Engineered Safety Feature (ESF) failure from a loop subsequent to LOCA: Assessment of plant vulnerability and CDF contributions

    SciTech Connect

    Martinez-Guridi, G.; Samanta, P.; Chu, L.; Yang, J.

    1998-03-01

    Generic Safety Issue 171 (GSI-171), Engineered Safety Feature (ESF) from a Loss Of Offsite Power (LOOP) subsequent to a Loss Of Coolant Accident (LOCA), deals with an accident sequence in which a LOCA is followed by a LOOP. This issue was later broadened to include a LOOP followed by a LOCA. Plants are designed to handle a simultaneous LOCA and LOOP. In this paper, the authors address the unique issues that are involved i LOCA with delayed LOOP (LOCA/LOOP) and LOOP with delayed LOCA (LOOP/LOCA) accident sequences. LOCA/LOOP accidents are analyzed further by developing event-tree/fault-tree models to quantify their contributions to core-damage frequency (CDF) in a pressurized water reactor and a boiling water reactor (PWR and a BWR). Engineering evaluation and judgments are used during quantification to estimate the unique conditions that arise in a LOCA/LOOP accident. The results show that the CDF contribution of such an accident can be a dominant contributor to plant risk, although BWRs are less vulnerable than PWRs.

  8. 75 FR 77694 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-13

    ... Register on November 26, 2010 (75 FR 72878), titled: ``Pipeline Safety: Updates to Pipeline and Liquefied... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477) or visit... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection...

  9. Clinicopathological features and surgical safety of gastric cancer in elderly patients.

    PubMed

    Lim, Joo Hyun; Lee, Dong Ho; Shin, Cheol Min; Kim, Nayoung; Park, Young Soo; Jung, Hyun Chae; Song, In Sung

    2014-12-01

    Gastric cancer is one of the most common cancers, especially among the elderly. However little is known about gastric cancer in elderly patients. This study was designed to evaluate the specific features of gastric cancer in elderly patients. Medical records of 1,107 patients who had radical gastrectomy for gastric cancer between June 2005 and December 2009 were reviewed. They were divided into young (<65 yr, n=676), young-old (65-74 yr, n=332), and old-old age group (≥75 yr, n=99). Increased CA 19-9 (5.6%, 13.4%, 14.6%, P=0.001), advanced diseases (42.5%, 47.0%, and 57.6, P=0.014), and node metastasis (37.6%, 38.9%, 51.5%, P=0.029) were more common in the young-old and old-old age groups. There were no significant differences in Helicobacter pylori status (63.6%, 56.7%, 61.2%, P=0.324) between the three groups. Surgery-related complication rates were similar in the three groups (5.3%, 5.1%, 8.1%, P=0.497). Microsatellite instability (P<0.001) and p53 overexpression (P<0.001) were more common among the elderly. The elderly group had more synchronous tumors (7.5%, 10.2%, 17.2%; P=0.006). Surgery can be applied to elderly gastric cancer without significant risk of complications. However, considering the more advanced disease and synchronous tumors among the elderly, care should be taken while deciding the extent of surgery for elderly gastric cancer.

  10. Feature Selection in Classification of Eye Movements Using Electrooculography for Activity Recognition

    PubMed Central

    Mala, S.; Latha, K.

    2014-01-01

    Activity recognition is needed in different requisition, for example, reconnaissance system, patient monitoring, and human-computer interfaces. Feature selection plays an important role in activity recognition, data mining, and machine learning. In selecting subset of features, an efficient evolutionary algorithm Differential Evolution (DE), a very efficient optimizer, is used for finding informative features from eye movements using electrooculography (EOG). Many researchers use EOG signals in human-computer interactions with various computational intelligence methods to analyze eye movements. The proposed system involves analysis of EOG signals using clearness based features, minimum redundancy maximum relevance features, and Differential Evolution based features. This work concentrates more on the feature selection algorithm based on DE in order to improve the classification for faultless activity recognition. PMID:25574185

  11. Critical features of an auditable management system for an ISO 9000-compatible occupational health and safety standard.

    PubMed

    Levine, S; Dyjack, D T

    1997-04-01

    An International Organization for Standardization (ISO) 9001: 1994-harmonized occupational health and safety (OHS) management system has been written at the University of Michigan, and reviewed, revised, and accepted under the direction of the American Industrial Hygiene Association (AIHA) Occupational Health and Safety Management Systems (OHSMS) Task Force and the Board of Directors. This system is easily adaptable to the ISO 14001 format and to both OHS and environmental management system applications. As was the case with ISO 9001: 1994, this system is expected to be compatible with current production quality and OHS quality systems and standards, have forward compatibility for new applications, and forward flexibility, with new features added as needed. Since ISO 9001: 1987 and 9001: 1994 have been applied worldwide, the incorporation of harmonized OHS and environmental management system components should be acceptable to business units already performing first-party (self-) auditing, and second-party (contract qualification) auditing. This article explains the basis of this OHS management system, its relationship to ISO 9001 and 14001 standards, the philosophy and methodology of an ISO-harmonized system audit, the relationship of these systems to traditional OHS audit systems, and the authors' vision of the future for application of such systems.

  12. Critical features of an auditable management system for an ISO 9000-compatible occupational health and safety standard.

    PubMed

    Levine, S; Dyjack, D T

    1997-04-01

    An International Organization for Standardization (ISO) 9001: 1994-harmonized occupational health and safety (OHS) management system has been written at the University of Michigan, and reviewed, revised, and accepted under the direction of the American Industrial Hygiene Association (AIHA) Occupational Health and Safety Management Systems (OHSMS) Task Force and the Board of Directors. This system is easily adaptable to the ISO 14001 format and to both OHS and environmental management system applications. As was the case with ISO 9001: 1994, this system is expected to be compatible with current production quality and OHS quality systems and standards, have forward compatibility for new applications, and forward flexibility, with new features added as needed. Since ISO 9001: 1987 and 9001: 1994 have been applied worldwide, the incorporation of harmonized OHS and environmental management system components should be acceptable to business units already performing first-party (self-) auditing, and second-party (contract qualification) auditing. This article explains the basis of this OHS management system, its relationship to ISO 9001 and 14001 standards, the philosophy and methodology of an ISO-harmonized system audit, the relationship of these systems to traditional OHS audit systems, and the authors' vision of the future for application of such systems. PMID:9115086

  13. Using Citizen Scientists to Gather, Analyze, and Disseminate Information About Neighborhood Features That Affect Active Living.

    PubMed

    Winter, Sandra J; Goldman Rosas, Lisa; Padilla Romero, Priscilla; Sheats, Jylana L; Buman, Matthew P; Baker, Cathleen; King, Abby C

    2016-10-01

    Many Latinos are insufficiently active, partly due to neighborhoods with little environmental support for physical activity. Multi-level approaches are needed to create health-promoting neighborhoods in disadvantaged communities. Participant "citizen scientists" were adolescent (n = 10, mean age = 12.8 ± 0.6 years) and older adult (n = 10, mean age = 71.3 ± 6.5 years), low income Latinos in North Fair Oaks, California. Citizen scientists conducted environmental assessments to document perceived barriers to active living using the Stanford Healthy Neighborhood Discovery Tool, which records GPS-tracked walking routes, photographs, audio narratives, and survey responses. Using a community-engaged approach, citizen scientists subsequently attended a community meeting to engage in advocacy training, review assessment data, prioritize issues to address and brainstorm potential solutions and partners. Citizen scientists each conducted a neighborhood environmental assessment and recorded 366 photographs and audio narratives. Adolescents (n = 4), older adults (n = 7) and community members (n = 4) collectively identified reducing trash and improving personal safety and sidewalk quality as the priority issues to address. Three adolescent and four older adult citizen scientists volunteered to present study findings to key stakeholders. This study demonstrated that with minimal training, low-income, Latino adolescent and older adult citizen scientists can: (1) use innovative technology to gather information about features of their neighborhood environment that influence active living, (2) analyze their information and identify potential solutions, and (3) engage with stakeholders to advocate for the development of healthier neighborhoods.

  14. Tectonic activity and structural features of active intracontinental normal faults in the Weihe Graben, central China

    NASA Astrophysics Data System (ADS)

    Rao, Gang; Lin, Aiming; Yan, Bing; Jia, Dong; Wu, Xiaojun

    2014-12-01

    This study examines the tectonic activity and structural features of active normal faults in the Weihe Graben, central China. The Weihe Graben is an area with a high level of historic seismicity, and it is one of the intracontinental systems that developed since Tertiary in the extensional environment around the Ordos Block. Analysis of high-resolution remote-sensing imagery data, field observations, and radiocarbon dating results reveal the following: i) active normal faults are mainly developed within a zone < 500 m wide along the southern border of the eastern part of the Weihe Graben; ii) the active faults that have been identified are characterized by stepwise fault scarps dipping into the graben at angles of 40°-71°; iii) there are numerous discontinuous individual fault traces, ranging in length from a few tens of meters to 450 m (generally < 200 m); iv) fault zone structures, topographic features, and fault striations on the main fault planes indicate almost pure normal-slip; and v) late Pleistocene-Holocene terrace risers, loess, and alluvial deposits have been vertically offset by up to ~ 80 m, with a non-uniform dip-slip rate (throw-rates) ranging from ~ 2.1 to 5.7 mm/yr, mostly 2-3 mm/yr. Our results reveal that active normal faults have been developing in the Weihe Graben under an ongoing extensional environment, probably associated with the pre-existing graben and spreading of the continental crust, and this is in contrast with the Ordos Block and neighboring orogenic regions. These results provide new insights into the nature of extensional tectonic deformation in intracontinental graben systems.

  15. Safety.

    ERIC Educational Resources Information Center

    Education in Science, 1996

    1996-01-01

    Discusses safety issues in science, including: allergic reactions to peanuts used in experiments; explosions in lead/acid batteries; and inspection of pressure vessels, such as pressure cookers or model steam engines. (MKR)

  16. MRI features of pediatric cerebral paragonimiasis in the active stage.

    PubMed

    Zhang, Jin Song; Huan, Yi; Sun, Li Jun; Zhang, Guang Yun; Ge, Ya Li; Zhao, Hai Tao

    2006-04-01

    We retrospectively reviewed the MR images of the brains of six children (age = 5-13 years) who had cerebral paragonimiasis in the early active stage. Diagnosis was based on a positive antibody test enzyme-linked immunosorbent assay (ELISA) for paragonimiasis in serum. The most common finding (in five patients) was irregular hemorrhage of various degrees. Moreover, in three cases some multiple irregular lesions with surrounding edema appeared to be conglomerated and aggregated. The rare appearance (in one patient) was a "tunnel sign," which showed the migrating track of the adult worm. In one patient with abscess and minimal hemorrhage, diffusion-weighted imaging (DWI) showed a heterogeneous high signal of lesions. Other findings included slight (one patient) or marked (one patient) irregular contrast enhancement, and large edematous areas surrounding small centers of hemorrhage (two patients). MR findings of conglomerated lesions with hemorrhage or tunnel sign may help to establish the diagnosis of active-stage cerebral paragonimiasis.

  17. Activities of the US-Japan Safety Monitor Joint Working Group

    SciTech Connect

    Richard L. Savercool; Lee C. Cadwallader

    2004-09-01

    This paper documents the activities of the US-Japan exchange in the area of personnel safety at magnetic and laser fusion experiments. A near-miss event with a visiting scientist to the US in 1992 was the impetus for forming the Joint Working Group on Fusion Safety. This exchnge has been under way for over ten years and has provided many safety insights for both US and Japanese facility personnel at national institutes and at universities. The background and activities of the Joint Working Group are described, including the facilities that have been visited for safety walkthroughs, the participants from both countries, and the main safety issues examined during visits. Based on these visits, some operational safety ideas to enhance experiment safety are given. The near-term future plans of the Safety Monitor Joint Working group are also discussed.

  18. 77 FR 46155 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-02

    ... information collection was published in the Federal Register on May 9, 2012, (77 FR 27279) under Docket No... April 11, 2000, (65 FR 19477) or visit http://www.regulations.gov before submitting any such comments... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection...

  19. 75 FR 13807 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-23

    ... Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477) or visit http... published in the Federal Register on July 2, 2009 (74 FR 31675) and comments were submitted to Docket No... Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information Collection...

  20. 75 FR 30099 - Pipeline Safety: Information Collection Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-28

    ... 23, 2010, (75 FR 13807). The agency did not receive any comments. The information collection is... Rulemaking for the One Rule was published in the Federal Register on July 2, 2009, (74 FR 31675) and comments... TRANSPORTATION Pipeline and Hazardous Materials Safety Administration Pipeline Safety: Information...

  1. Convoy active safety technologies war fighter experiment II

    NASA Astrophysics Data System (ADS)

    Schoenherr, Edward W.

    2009-01-01

    The operational ability to project and sustain forces in distant, anti-access and area denial environments poses new challenges for combatant commanders. One of the new challenges is the ability to conduct sustainment operations at operationally feasible times and places on the battlefield. Combatant commanders require a sustainment system that is agile, versatile, and survivable throughout the range of military operations and across the spectrum of conflict. A key component of conducting responsive, operationally feasible sustainment operations is the ability to conduct sustainment convoys. Sustainment convoys are critical to providing combatant commanders the right support, at the right time and place, and in the right quantities, across the full range of military operations. The ability to conduct sustainment convoys in a variety of hostile environments require force protection measures that address the enemy threat and protect the Soldier. One cost effective, technically feasible method of increasing the force protection for sustainment convoys is the use of robotic follower technology and autonomous navigation. The Convoy Active Safety Technologies (CAST) system is a driver assist, convoy autopilot technology aimed to address these issues. The CAST Warfigher Experiment II, being held at The Nevada Automotive Test Center in the fall of 2008, will continue analysis of the utility of this vehicle following technology not only in measures of system integrity and performance vs. manual driving, but also the physiological effects on the operators themselves. This paper will detail this experiment's methodology and analysis. Results will be presented at the SPIE Electronic Imaging 2009 symposium.

  2. Features of the Built Environment Related to Physical Activity Friendliness and Children’s Obesity and Other Risk Factors

    PubMed Central

    Taylor, Wendell C.; Upchurch, Sandra L.; Brosnan, Christine A.; Selwyn, Beatrice J.; Nguyen, Thong Q.; Villagomez, Evangelina Trejo; Meininger, Janet C.

    2014-01-01

    Objectives We investigated the relationships among environmental features of physical activity friendliness, socioeconomic indicators, and prevalence of obesity (BMI status), central adiposity (waist circumference, waist-height ratio), and hypertension. Design The design was cross-sectional; the study was correlational. Sample The sample was 911 kindergarteners through sixth graders from three schools in an urban school district residing in 13 designated neighborhoods. Measurements Data from walking environmental community audits, census data for socioeconomic indicators, body mass index, waist circumference, waist-height ratio, and blood pressure were analyzed. A modified Alfonzo’s Hierarchy of Walking Needs model was the conceptual framework for environmental features (i.e., accessibility, safety, comfort, and pleasurability) related to physical activity. Results Accessibility was significantly and negatively correlated with prevalence of obesity and with prevalence of a waist-height ratio > 0.50. When neighborhood education was controlled, and when both neighborhood education and poverty were controlled with partial correlational analysis, comfort features of a walking environment were significantly and positively related to prevalence of obesity. When poverty was controlled with partial correlation, accessibility was significantly and negatively correlated with prevalence of waist-height ratio > 0.50. Conclusions The built environment merits further research to promote physical activity and stem the obesity epidemic in children. Our approach can be a useful framework for future research. PMID:25112374

  3. A Rodent Model of Chikungunya Virus Infection in RAG1 -/- Mice, with Features of Persistence, for Vaccine Safety Evaluation.

    PubMed

    Seymour, Robert L; Adams, A Paige; Leal, Grace; Alcorn, Maria D H; Weaver, Scott C

    2015-01-01

    Chikungunya virus (CHIKV) is a positive sense, single stranded RNA virus in the genus Alphavirus, and the etiologic agent of epidemics of severe arthralgia in Africa, Asia, Europe and, most recently, the Americas. CHIKV causes chikungunya fever (CHIK), a syndrome characterized by rash, fever, and debilitating, often chronic arthritis. In recent outbreaks, CHIKV has been recognized to manifest more neurologic signs of illness in the elderly and those with co-morbidities. The syndrome caused by CHIKV is often self-limited; however, many patients develop persistent arthralgia that can last for months or years. These characteristics make CHIKV not only important from a human health standpoint, but also from an economic standpoint. Despite its importance as a reemerging disease, there is no licensed vaccine or specific treatment to prevent CHIK. Many studies have begun to elucidate the pathogenesis of CHIKF and the mechanism of persistent arthralgia, including the role of the adaptive immune response, which is still poorly understood. In addition, the lack of an animal model for chronic infection has limited studies of CHIKV pathogenesis as well as the ability to assess the safety of vaccine candidates currently under development. To address this deficiency, we used recombination activating gene 1 (RAG1-/-) knockout mice, which are deficient in both T and B lymphocytes, to develop a chronic CHIKV infection model. Here, we describe this model as well as its use in evaluating the safety of a live-attenuated vaccine candidate.

  4. Identification of resting and active state EEG features of Alzheimer's disease using discrete wavelet transform.

    PubMed

    Ghorbanian, Parham; Devilbiss, David M; Verma, Ajay; Bernstein, Allan; Hess, Terry; Simon, Adam J; Ashrafiuon, Hashem

    2013-06-01

    Alzheimer's disease (AD) is associated with deficits in a number of cognitive processes and executive functions. Moreover, abnormalities in the electroencephalogram (EEG) power spectrum develop with the progression of AD. These features have been traditionally characterized with montage recordings and conventional spectral analysis during resting eyes-closed and resting eyes-open (EO) conditions. In this study, we introduce a single lead dry electrode EEG device which was employed on AD and control subjects during resting and activated battery of cognitive and sensory tasks such as Paced Auditory Serial Addition Test (PASAT) and auditory stimulations. EEG signals were recorded over the left prefrontal cortex (Fp1) from each subject. EEG signals were decomposed into sub-bands approximately corresponding to the major brain frequency bands using several different discrete wavelet transforms and developed statistical features for each band. Decision tree algorithms along with univariate and multivariate statistical analysis were used to identify the most predictive features across resting and active states, separately and collectively. During resting state recordings, we found that the AD patients exhibited elevated D4 (~4-8 Hz) mean power in EO state as their most distinctive feature. During the active states, however, the majority of AD patients exhibited larger minimum D3 (~8-12 Hz) values during auditory stimulation (18 Hz) combined with increased kurtosis of D5 (~2-4 Hz) during PASAT with 2 s interval. When analyzed using EEG recording data across all tasks, the most predictive AD patient features were a combination of the first two feature sets. However, the dominant discriminating feature for the majority of AD patients were still the same features as the active state analysis. The results from this small sample size pilot study indicate that although EEG recordings during resting conditions are able to differentiate AD from control subjects, EEG activity

  5. Auto Mechanics I. Learning Activity Packets (LAPs). Section A--Orientation and Safety.

    ERIC Educational Resources Information Center

    Oklahoma State Board of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

    This document contains seven learning activity packets (LAPs) that outline the study activities for the orientation and safety instructional area for an Auto Mechanics I course. The seven LAPs cover the following topics: orientation, safety, hand tools, arc welding, oxyacetylene cutting, oxyacetylene fusion welding, and oxyacetylene braze welding.…

  6. Function Follows Form: Activation of Shape and Function Features during Object Identification

    ERIC Educational Resources Information Center

    Yee, Eiling; Huffstetler, Stacy; Thompson-Schill, Sharon L.

    2011-01-01

    Most theories of semantic memory characterize knowledge of a given object as comprising a set of semantic features. But how does conceptual activation of these features proceed during object identification? We present the results of a pair of experiments that demonstrate that object recognition is a dynamically unfolding process in which function…

  7. Identifying Key Features of Effective Active Learning: The Effects of Writing and Peer Discussion

    ERIC Educational Resources Information Center

    Linton, Debra L.; Pangle, Wiline M.; Wyatt, Kevin H.; Powell, Karli N.; Sherwood, Rachel E.

    2014-01-01

    We investigated some of the key features of effective active learning by comparing the outcomes of three different methods of implementing active-learning exercises in a majors introductory biology course. Students completed activities in one of three treatments: discussion, writing, and discussion + writing. Treatments were rotated weekly between…

  8. Operation Safety Activities for JEM System and Payload Operation

    NASA Astrophysics Data System (ADS)

    Takada, Satomi; Iwata, Yoshihiro; Kato, Mitsuyasu

    2010-09-01

    The Japanese Experiment Module(JEM), "KIBO", which is a part of the International Space Station(ISS) is the first Japanese manned space experimental facility. JEM system and payloads have made the birth of an era of operation. The JAXA Human Space S&MA(JAXA S&MA) assures safety of JEM module and JAXA payloads not only during assembly phase but also operation phase. During the safety critical operation for JEM system and payloads, JAXA S&MA is on ESR S&MA console to monitor the operation related to safety. Safety check list is made for each safety critical task to identify the useful information such as hazard control, operational constraints and flight rules, and so on. It is a support tool for JAXA S&MA to monitor the operation overall. JAXA S&MA has the responsibility of assessing the safety related updates or changes of operational documents. JAXA S&MA will continue to support the JEM operation as long as the operation is continued.

  9. OBSERVED, GIS, AND SELF-REPORTED ENVIRONMENTAL FEATURES AND ADOLESCENT PHYSICAL ACTIVITY

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Purpose: Examine associations among observed, self-reported, and Geographical Information Systems (GIS) environmental features and physical activity among adolescent males. Design: Cross-sectional study. Setting: Boy Scout troops and neighborhoods in Houston, Texas. Subjects: Two hundred and ten ...

  10. Control-Plate Regression (CPR) Normalization for High-Throughput Screens with Many Active Features.

    PubMed

    Murie, C; Barette, C; Lafanechère, L; Nadon, R

    2014-06-01

    Systematic error is present in all high-throughput screens, lowering measurement accuracy. Because screening occurs at the early stages of research projects, measurement inaccuracy leads to following up inactive features and failing to follow up active features. Current normalization methods take advantage of the fact that most primary-screen features (e.g., compounds) within each plate are inactive, which permits robust estimates of row and column systematic-error effects. Screens that contain a majority of potentially active features pose a more difficult challenge because even the most robust normalization methods will remove at least some of the biological signal. Control plates that contain the same feature in all wells can provide a solution to this problem by providing well-by-well estimates of systematic error, which can then be removed from the treatment plates. We introduce the robust control-plate regression (CPR) method, which uses this approach. CPR's performance is compared to a high-performing primary-screen normalization method in four experiments. These data were also perturbed to simulate screens with large numbers of active features to further assess CPR's performance. CPR performs almost as well as the best performing normalization methods with primary screens and outperforms the Z-score and equivalent methods with screens containing a large proportion of active features.

  11. Does perceived neighborhood walkability and safety mediate the association between education and meeting physical activity guidelines?

    PubMed

    Pratt, Michael; Yin, Shaoman; Soler, Robin; Njai, Rashid; Siegel, Paul Z; Liao, Youlian

    2015-04-09

    The role of neighborhood walkability and safety in mediating the association between education and physical activity has not been quantified. We used data from the 2010 and 2012 Communities Putting Prevention to Work Behavioral Risk Factor Surveillance System and structural equation modeling to estimate how much of the effect of education level on physical activity was mediated by perceived neighborhood walkability and safety. Neighborhood walkability accounts for 11.3% and neighborhood safety accounts for 6.8% of the effect. A modest proportion of the important association between education and physical activity is mediated by perceived neighborhood walkability and safety, suggesting that interventions focused on enhancing walkability and safety could reduce the disparity in physical activity associated with education level.

  12. Human activity recognition based on feature selection in smart home using back-propagation algorithm.

    PubMed

    Fang, Hongqing; He, Lei; Si, Hao; Liu, Peng; Xie, Xiaolei

    2014-09-01

    In this paper, Back-propagation(BP) algorithm has been used to train the feed forward neural network for human activity recognition in smart home environments, and inter-class distance method for feature selection of observed motion sensor events is discussed and tested. And then, the human activity recognition performances of neural network using BP algorithm have been evaluated and compared with other probabilistic algorithms: Naïve Bayes(NB) classifier and Hidden Markov Model(HMM). The results show that different feature datasets yield different activity recognition accuracy. The selection of unsuitable feature datasets increases the computational complexity and degrades the activity recognition accuracy. Furthermore, neural network using BP algorithm has relatively better human activity recognition performances than NB classifier and HMM.

  13. RELATIONSHIPS BETWEEN GIS ENVIRONMENTAL FEATURES AND ADOLESCENT MALE PHYSICAL ACTIVITY: GIS CODING DIFFERENCES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: It is not clear if relationships between GIS obtained environmental features and physical activity differ according to the method used to code GIS data. Methods: Physical activity levels of 210 Boy Scouts were measured by accelerometer. Numbers of parks, trails, gymnasia, bus stops, groc...

  14. Active shape models with invariant optimal features: application to facial analysis.

    PubMed

    Sukno, Federico M; Ordás, Sebastián; Butakoff, Constantine; Cruz, Santiago; Frangi, Alejandro F

    2007-07-01

    This work is framed in the field of statistical face analysis. In particular, the problem of accurate segmentation of prominent features of the face in frontal view images is addressed. We propose a method that generalizes linear Active Shape Models (ASMs), which have already been used for this task. The technique is built upon the development of a nonlinear intensity model, incorporating a reduced set of differential invariant features as local image descriptors. These features are invariant to rigid transformations, and a subset of them is chosen by Sequential Feature Selection for each landmark and resolution level. The new approach overcomes the unimodality and Gaussianity assumptions of classical ASMs regarding the distribution of the intensity values across the training set. Our methodology has demonstrated a significant improvement in segmentation precision as compared to the linear ASM and Optimal Features ASM (a nonlinear extension of the pioneer algorithm) in the tests performed on AR, XM2VTS, and EQUINOX databases. PMID:17496371

  15. Energy and Safety: Science Activities for Elementary Students, Level I (Grades (K-2).

    ERIC Educational Resources Information Center

    Westcott, Dale; And Others

    Twelve activities are presented that focus on a common phenomenon of a child's world: energy. These activities relate energy, how it occurs, how it is used, and how to use it safely. Each activity includes the purpose, introduction, background, procedure, materials, estimated time for the activity, typical results, safety notes, and more ideas.…

  16. Energy and Safety: Science Activities for Elementary Students, Level III (Grades (5-6).

    ERIC Educational Resources Information Center

    Westcott, Dale; And Others

    Thirteen activities are presented that focus on a common phenomenon of a child's world: energy. These activities relate energy, how it occurs, how it is used, and how to use it safely. Each activity includes the purpose, introduction, background, procedure, materials, estimated time for the activity, typical results, safety notes, and more ideas.…

  17. Energy and Safety: Science Activities for Elementary Students, Level II (Grades (3-4).

    ERIC Educational Resources Information Center

    Westcott, Dale; And Others

    Thirteen activities are presented that focus on a common phenomenon of a child's world: energy. These activities relate energy, how it occurs, how it is used, and how to use it safely. Each activity includes the purpose, introduction, background, procedure, materials, estimated time for the activity, typical results, safety notes, and more ideas.…

  18. Feature activation during word recognition: action, visual, and associative-semantic priming effects

    PubMed Central

    Lam, Kevin J. Y.; Dijkstra, Ton; Rueschemeyer, Shirley-Ann

    2015-01-01

    Embodied theories of language postulate that language meaning is stored in modality-specific brain areas generally involved in perception and action in the real world. However, the temporal dynamics of the interaction between modality-specific information and lexical-semantic processing remain unclear. We investigated the relative timing at which two types of modality-specific information (action-based and visual-form information) contribute to lexical-semantic comprehension. To this end, we applied a behavioral priming paradigm in which prime and target words were related with respect to (1) action features, (2) visual features, or (3) semantically associative information. Using a Go/No-Go lexical decision task, priming effects were measured across four different inter-stimulus intervals (ISI = 100, 250, 400, and 1000 ms) to determine the relative time course of the different features. Notably, action priming effects were found in ISIs of 100, 250, and 1000 ms whereas a visual priming effect was seen only in the ISI of 1000 ms. Importantly, our data suggest that features follow different time courses of activation during word recognition. In this regard, feature activation is dynamic, measurable in specific time windows but not in others. Thus the current study (1) demonstrates how multiple ISIs can be used within an experiment to help chart the time course of feature activation and (2) provides new evidence for embodied theories of language. PMID:26074836

  19. 78 FR 50079 - Information Collection Activities: Safety and Environmental Management Systems (SEMS); Proposed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-16

    ...: 134E1700D2 EEEE500000 ET1SF0000.DAQ000] Information Collection Activities: Safety and Environmental Management Systems (SEMS); Proposed Collection; Comment Request Correction In notice document...

  20. Safety assessment of discharge chute isolation barrier preparation and installation activities. Revision 3

    SciTech Connect

    Meichle, R.H.

    1994-11-08

    This revision adds a section addressing impacts of dropping surfacing tool and rack cutter on the basin floor, and corrects typographical errors. The safety assessment is made for the activities for the preparation and installation of the discharge chute isolation barriers. The safety assessment includes a hazard assessment and comparisons of potential accidents/events to those addressed by the current safety basis documentation. No significant hazards were identified. An evaluation against the USQ evaluation questions was made and the determination made that the activities do not represent a USQ. Hazard categorization techniques were used to provide a basis for readiness review classifications.

  1. From Monty Python to Total Recall: A Feature Film Activity for the Cognitive Psychology Course.

    ERIC Educational Resources Information Center

    Conner, David B.

    1996-01-01

    Describes a college psychology course activity designed to help students define the parameters of cognitive psychology. Students selected a feature film and a journal article that represented some aspect of cognitive psychology. They then wrote a paper discussing the theoretical and empirical connections between the sources and the topic. (MJP)

  2. Three steps to safety: developing procedures for active shooters.

    PubMed

    Morris, Lisa W

    2014-01-01

    Every Second counts once gunshots are heard in the workplace environment. Close the office door, turn out the lights and turn the mobile phone to 'silent' is the standard mantra for what is expected in the response efforts; however, is that enough? In a perfect world, this sermon may fall short of what emergency management practitioners might preach as it does not adequately fulfil the reality of what is best practice for optimal life safety. This paper offers options for lockdown preparedness and response to address internal lockdown from the moment shots are fired. Recommendations for the creation of a lockdown plan, building assessment surveys and a controlled, simulated exercise are addressed to raise awareness in response methods and reduce overall response time. The procedures suggested in this paper will optimise training efforts using the community's standard emergency operating procedures in response to workplace violence to minimise loss of life.

  3. Enhancing the NASA Expendable Launch Vehicle Payload Safety Review Process Through Program Activities

    NASA Technical Reports Server (NTRS)

    Palo, Thomas E.

    2007-01-01

    The safety review process for NASA spacecraft flown on Expendable Launch Vehicles (ELVs) has been guided by NASA-STD 8719.8, Expendable Launch Vehicle Payload Safety Review Process Standard. The standard focused primarily on the safety approval required to begin pre-launch processing at the launch site. Subsequent changes in the contractual, technical, and operational aspects of payload processing, combined with lessons-learned supported a need for the reassessment of the standard. This has resulted in the formation of a NASA ELV Payload Safety Program. This program has been working to address the programmatic issues that will enhance and supplement the existing process, while continuing to ensure the safety of ELV payload activities.

  4. Low activity of plasminogen activator: a common feature of non- iatrogenic comorbidities of schizophrenia.

    PubMed

    Hoirisch-Clapauch, Silvia; Nardi, Antonio E

    2015-01-01

    Understanding the pathogenesis of non-iatrogenic comorbidities of schizophrenia may provide insights into the pathogenesis of schizophrenia itself. First-episode, drug-naïve schizophrenia patients are at high risk of thromboembolic events, diseases related to substance abuse, sexual dysfunction, reproductive disorders, inflammatory and autoimmune diseases, as well as complications of hyperinsulinemia or hyperhomocysteinemia. This review focuses on the role of reduced plasminogen activator activity in non-iatrogenic comorbidity of schizophrenia. By preventing thrombus dissolution, low tissue plasminogen activator activity increases the risk of thrombotic events. Components of the plasminogen activator system also play a key role in reproduction. Both illicit drugs and tobacco increase plasminogen activator levels in the central nervous system, which seems to relieve symptoms of the mental disorder. Chronic alcoholism, sexual dysfunction, inflammatory and autoimmune disorders, and complications of hyperinsulinemia or hyperhomocysteinemia are somehow related to low plasminogen activator activity. Plasminogen activator mediates several neurochemical processes that seem to prevent or reverse gray-matter atrophy seen in first-episode schizophrenia patients. Such processes include cleavage of brain-derived neurotrophic factor precursor to an anti-apoptotic neurotrophin and activation of N-methyl-D-aspartate receptor. Controlled, randomized studies are needed to determine if measures aimed at correcting plasminogen activator activity can improve the quality of life, reduce morbidity and mortality rates, and particularly improve the course of schizophrenia. PMID:25714971

  5. Using Activity-Related Behavioural Features towards More Effective Automatic Stress Detection

    PubMed Central

    Giakoumis, Dimitris; Drosou, Anastasios; Cipresso, Pietro; Tzovaras, Dimitrios; Hassapis, George; Gaggioli, Andrea; Riva, Giuseppe

    2012-01-01

    This paper introduces activity-related behavioural features that can be automatically extracted from a computer system, with the aim to increase the effectiveness of automatic stress detection. The proposed features are based on processing of appropriate video and accelerometer recordings taken from the monitored subjects. For the purposes of the present study, an experiment was conducted that utilized a stress-induction protocol based on the stroop colour word test. Video, accelerometer and biosignal (Electrocardiogram and Galvanic Skin Response) recordings were collected from nineteen participants. Then, an explorative study was conducted by following a methodology mainly based on spatiotemporal descriptors (Motion History Images) that are extracted from video sequences. A large set of activity-related behavioural features, potentially useful for automatic stress detection, were proposed and examined. Experimental evaluation showed that several of these behavioural features significantly correlate to self-reported stress. Moreover, it was found that the use of the proposed features can significantly enhance the performance of typical automatic stress detection systems, commonly based on biosignal processing. PMID:23028461

  6. Safety Is No Accident: Children's Activities in Injury Prevention.

    ERIC Educational Resources Information Center

    Kane, William M.; Herrera, Kathleen E.

    This book is one in the Children's Activity Series of books to promote awareness about health, family life, and cultural diversity for children in kindergarten through third grade. The books in this series are written to enhance an established curriculum, rather than to serve as the curriculum itself. They offer ideas for hands-on activities for…

  7. Playability: Built and Social Environment Features That Promote Physical Activity Within Children.

    PubMed

    Timperio, Anna; Reid, Jacqueline; Veitch, Jenny

    2015-12-01

    The role of neighbourhood built and social environments in shaping children's physical activity has received increasing interest over the past 10 years. We reviewed recent evidence published between 2011 and 2014. Most of the recent evidence continues to be cross-sectional. Few macro-level neighbourhood attributes were consistently associated with physical activity in the expected direction. The strongest evidence for associations between neighbourhood attributes and physical activity with was for the transportation environment, particularly in relation to proximity to school and transport-related physical activity. There was intermediate evidence that neighbourhood walking/cycling infrastructure and pedestrian safety structures are associated with transport-related PA. Recent evidence on associations between the neighbourhood built and social environment and children's PA is modest. Stronger study designs and greater attention to conceptual-matching and specificity of measures are critical to advance the evidence base.

  8. FUNCTION FOLLOWS FORM: ACTIVATION OF SHAPE & FUNCTION FEATURES DURING OBJECT IDENTIFICATION

    PubMed Central

    Yee, Eiling; Huffstetler, Stacy; Thompson-Schill, Sharon L.

    2011-01-01

    Most theories of semantic memory characterize knowledge of a given object as comprising a set of semantic features. But how does conceptual activation of these features proceed during object identification? We present the results of a pair of experiments that demonstrate that object recognition is a dynamically unfolding process in which function follows form. We used eye movements to explore whether activating one object’s concept leads to the activation of others that share perceptual (shape) or abstract (function) features. Participants viewed four-picture displays and clicked on the picture corresponding to a heard word. In critical trials, the conceptual representation of one of the objects in the display was similar in shape or function (i.e., its purpose) to the heard word. Importantly, this similarity was not apparent in the visual depictions (e.g., for the target “frisbee,” the shape-related object was a triangular slice of pizza – a shape that a frisbee cannot take); preferential fixations on the related object were therefore attributable to overlap of the conceptual representations on the relevant features. We observed relatedness effects for both shape and function, but shape effects occurred earlier than function effects. We discuss the implications of these findings for current accounts of the representation of semantic memory. PMID:21417543

  9. Multifractal features of magnetospheric dynamics and their dependence on solar activity

    NASA Astrophysics Data System (ADS)

    Gopinath, Sumesh

    2016-09-01

    In the present study, novel wavelet leaders (WL) based multifractal analysis has been used to get a better knowledge of the self-organization phenomena inherent in complex magnetospheric dynamics during disturbance and quiescent periods, focusing mainly on the intermittent features of auroral electrojet (AE) index. The results derived from the analysis certainly exhibit the phase transition property of magnetosphere system with respect to variabilities in the driving conditions. By using the novel WL method, solar activity dependence/independence of intermittency of magnetospheric proxies such as AE, SYM-H and Dst indices have been compared. The results indicate that the multifractality of AE index does not follow the solar activity cycle while intermittent features of SYM-H and Dst indices show high degree of solar activity dependence. This shows that along with the external solar wind perturbations, certain complex phenomena of internal origin also significantly modulate the dynamics of geomagnetic fluctuations in the auroral region.

  10. Safety enhancement of oil trunk pipeline crossing active faults on Sakhalin Island

    NASA Astrophysics Data System (ADS)

    Tishkina, E.; Antropova, N.; Korotchenko, T.

    2015-11-01

    The article explores the issues concerning safety enhancement of pipeline active fault crossing on Sakhalin Island. Based on the complexity and analysis results, all the faults crossed by pipeline system are classified into five categories - from very simple faults to extremely complex ones. The pipeline fault crossing design is developed in accordance with the fault category. To enhance pipeline safety at fault crossing, a set of methods should be applied: use of pipes of different safety classes and special trench design in accordance with soil permeability characteristics.

  11. System safety activities supporting an aero-space plane ground support technology

    NASA Technical Reports Server (NTRS)

    Mattern, Steven F.

    1992-01-01

    An overview is presented of the specific system safety activities required to support the ground support technology program associated with the design of an aerospace plane. Safe zones must be assessed to ensure that explosive safety requirements are attained to protect the vehicle, personnel, and support and operational facilities. Attention is given to the specific and unique design requirements connected with the utilization of cryogenic fuels as they apply to the design and development of an aerospace plane.

  12. Factors associated with the activities of safety representatives in Spanish workplaces

    PubMed Central

    García, Ana M; López‐Jacob, Maria José; Dudzinski, Isabel; Gadea, Rafael; Rodrigo, Fernando

    2007-01-01

    Objective To describe the activities of safety representatives in workplaces in Spain. Methods A specific questionnaire was applied to a representative sample of safety representatives. Activities developed during the past year, presented in a closed ended list, were categorised into three groups: information and advising; participation in occupational health management; and pressure on or negotiation with employers. Personal phone interviews were conducted from September to December 2004. A sample of 1201 interviews was attained. Crude and multivariate analyses were carried out. Results Spanish safety representatives were mostly men (76%), aged 26 to 45 years (62%), with fixed contracts (94%), and more than 10 years in their company (57%). On a comparable 0–10 scale, the mean (95% confidence interval (CI)) number of activities relating to information, management, and negotiation developed during the previous year were, respectively, 6.8 (6.7 to 6.9), 4.5 (4.4 to 4.7), and 4.0 (3.8 to 4.1). In multivariate analysis, workplace size (>30 workers), industrial sector, training, and support from the labour inspectorate were the factors most consistently associated with safety representatives' activity. Additionally, support from the employer was associated with participation in occupational health management (odds ratio = 2.38 (95% CI, 1.73 to 3.29)). Conclusions Safety representatives in Spain have a variety of activities, mostly in the category of information and advising. These are necessary but not sufficient for real participation of workers in decisions concerning their health and safety. More participation of safety representatives in occupational health management at workplaces seems to be needed, and factors associated with this participation reinforced. PMID:17699532

  13. Addressing the implementation gap in global road safety: exploring features of an effective response and introducing a 10-country program.

    PubMed

    Hyder, Adnan A; Allen, Katharine A; Di Pietro, Gayle; Adriazola, Claudia A; Sobel, Rochelle; Larson, Kelly; Peden, Margie

    2012-06-01

    Yearly, more than 1.2 million people are killed by road traffic injuries (RTIs) around the globe, and another 20 to 50 million are injured. The global burden of RTIs is predicted to rise. We explored the need for concerted action for global road safety and propose characteristics of an effective response to the gap in addressing RTIs. We propose that a successful response includes domains such as strong political will, capacity building, use of evidence-based interventions, rigorous evaluation, increased global funding, multisectoral action, and sustainability. We also present a case study of the global Road Safety in 10 Countries project, which is a new, 5-year, multipartner initiative to address the burden of RTIs in 10 low- and middle-income countries.

  14. Addressing the Implementation Gap in Global Road Safety: Exploring Features of an Effective Response and Introducing a 10-Country Program

    PubMed Central

    Allen, Katharine A.; Di Pietro, Gayle; Adriazola, Claudia A.; Sobel, Rochelle; Larson, Kelly; Peden, Margie

    2012-01-01

    Yearly, more than 1.2 million people are killed by road traffic injuries (RTIs) around the globe, and another 20 to 50 million are injured. The global burden of RTIs is predicted to rise. We explored the need for concerted action for global road safety and propose characteristics of an effective response to the gap in addressing RTIs. We propose that a successful response includes domains such as strong political will, capacity building, use of evidence-based interventions, rigorous evaluation, increased global funding, multisectoral action, and sustainability. We also present a case study of the global Road Safety in 10 Countries project, which is a new, 5-year, multipartner initiative to address the burden of RTIs in 10 low- and middle-income countries. PMID:22515864

  15. Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities.

    PubMed

    Ferreira, Isabel C F R; Heleno, Sandrina A; Reis, Filipa S; Stojkovic, Dejan; Queiroz, Maria João R P; Vasconcelos, M Helena; Sokovic, Marina

    2015-06-01

    Ganoderma genus comprises one of the most commonly studied species worldwide, Ganoderma lucidum. However, other Ganoderma species have been also reported as important sources of bioactive compounds. Polysaccharides are important contributors to the medicinal properties reported for Ganoderma species, as demonstrated by the numerous publications, including reviews, on this matter. Yet, what are the chemical features of Ganoderma polysaccharides that have bioactivity? In the present manuscript, the chemical features of Ganoderma polysaccharides with reported antioxidant, antitumor and antimicrobial activities (the most studied worldwide) are analyzed in detail. The composition of sugars (homo- versus hetero-glucans and other polysaccharides), type of glycosidic linkages, branching patterns, and linkage to proteins are discussed. Methods for extraction, isolation and identification are evaluated and, finally, the bioactivity of polysaccharidic extracts and purified compounds are discussed. The integration of data allows deduction of structure-activity relationships and gives clues to the chemical aspects involved in Ganoderma bioactivity.

  16. Safety update on the use of recombinant activated factor VII in approved indications.

    PubMed

    Neufeld, Ellis J; Négrier, Claude; Arkhammar, Per; Benchikh el Fegoun, Soraya; Simonsen, Mette Duelund; Rosholm, Anders; Seremetis, Stephanie

    2015-06-01

    This updated safety review summarises the large body of safety data available on the use of recombinant activated factor VII (rFVIIa) in approved indications: haemophilia with inhibitors, congenital factor VII (FVII) deficiency, acquired haemophilia and Glanzmann's thrombasthenia. Accumulated data up to 31 December 2013 from clinical trials as well as post-marketing data (registries, literature reports and spontaneous reports) were included. Overall, rFVIIa has shown a consistently favourable safety profile, with no unexpected safety concerns, in all approved indications. No confirmed cases of neutralising antibodies against rFVIIa have been reported in patients with congenital haemophilia, acquired haemophilia or Glanzmann's thrombasthenia. The favourable safety profile of rFVIIa can be attributed to the recombinant nature of rFVIIa and its localised mechanism of action at the site of vascular injury. Recombinant FVIIa activates factor X directly on the surface of activated platelets, which are present only at the site of injury, meaning that systemic activation of coagulation is avoided and the risk of thrombotic events (TEs) thus reduced. Nonetheless, close monitoring for signs and symptoms of TE is warranted in all patients treated with any pro-haemostatic agent, including rFVIIa, especially the elderly and any other patients with concomitant conditions and/or predisposing risk factors to thrombosis.

  17. NASA safety program activities in support of the Space Exploration Initiatives Nuclear Propulsion program

    NASA Technical Reports Server (NTRS)

    Sawyer, J. C., Jr.

    1993-01-01

    The activities of the joint NASA/DOE/DOD Nuclear Propulsion Program Technical Panels have been used as the basis for the current development of safety policies and requirements for the Space Exploration Initiatives (SEI) Nuclear Propulsion Technology development program. The Safety Division of the NASA Office of Safety and Mission Quality has initiated efforts to develop policies for the safe use of nuclear propulsion in space through involvement in the joint agency Nuclear Safety Policy Working Group (NSPWG), encouraged expansion of the initial policy development into proposed programmatic requirements, and suggested further expansion into the overall risk assessment and risk management process for the NASA Exploration Program. Similar efforts are underway within the Department of Energy to ensure the safe development and testing of nuclear propulsion systems on Earth. This paper describes the NASA safety policy related to requirements for the design of systems that may operate where Earth re-entry is a possibility. The expected plan of action is to support and oversee activities related to the technology development of nuclear propulsion in space, and support the overall safety and risk management program being developed for the NASA Exploration Program.

  18. DOE standard: Integration of environment, safety, and health into facility disposition activities. Volume 1: Technical standard

    SciTech Connect

    1998-05-01

    This Department of Energy (DOE) technical standard (referred to as the Standard) provides guidance for integrating and enhancing worker, public, and environmental protection during facility disposition activities. It provides environment, safety, and health (ES and H) guidance to supplement the project management requirements and associated guidelines contained within DOE O 430.1A, Life-Cycle Asset Management (LCAM), and amplified within the corresponding implementation guides. In addition, the Standard is designed to support an Integrated Safety Management System (ISMS), consistent with the guiding principles and core functions contained in DOE P 450.4, Safety Management System Policy, and discussed in DOE G 450.4-1, Integrated Safety Management System Guide. The ISMS guiding principles represent the fundamental policies that guide the safe accomplishment of work and include: (1) line management responsibility for safety; (2) clear roles and responsibilities; (3) competence commensurate with responsibilities; (4) balanced priorities; (5) identification of safety standards and requirements; (6) hazard controls tailored to work being performed; and (7) operations authorization. This Standard specifically addresses the implementation of the above ISMS principles four through seven, as applied to facility disposition activities.

  19. Music-induced emotions can be predicted from a combination of brain activity and acoustic features.

    PubMed

    Daly, Ian; Williams, Duncan; Hallowell, James; Hwang, Faustina; Kirke, Alexis; Malik, Asad; Weaver, James; Miranda, Eduardo; Nasuto, Slawomir J

    2015-12-01

    It is widely acknowledged that music can communicate and induce a wide range of emotions in the listener. However, music is a highly-complex audio signal composed of a wide range of complex time- and frequency-varying components. Additionally, music-induced emotions are known to differ greatly between listeners. Therefore, it is not immediately clear what emotions will be induced in a given individual by a piece of music. We attempt to predict the music-induced emotional response in a listener by measuring the activity in the listeners electroencephalogram (EEG). We combine these measures with acoustic descriptors of the music, an approach that allows us to consider music as a complex set of time-varying acoustic features, independently of any specific music theory. Regression models are found which allow us to predict the music-induced emotions of our participants with a correlation between the actual and predicted responses of up to r=0.234,p<0.001. This regression fit suggests that over 20% of the variance of the participant's music induced emotions can be predicted by their neural activity and the properties of the music. Given the large amount of noise, non-stationarity, and non-linearity in both EEG and music, this is an encouraging result. Additionally, the combination of measures of brain activity and acoustic features describing the music played to our participants allows us to predict music-induced emotions with significantly higher accuracies than either feature type alone (p<0.01).

  20. A new semi-active safety control strategy for high-speed railway vehicles

    NASA Astrophysics Data System (ADS)

    Guo, Jin; Xu, Zhengguo; Sun, Youxian

    2015-12-01

    This paper focuses on the safety of railway vehicles. A new semi-active control strategy is proposed based on the skyhook control theory. In view of the main railway vehicle safety performance indicators, the new control strategy aims at reducing the derailment coefficient of railway vehicles by restraining the lateral vibrations of the bogie and the wheelset. Furthermore, to evaluate the improvement of the safety performance brought about by the new control strategy, a complete railway vehicle model is established using the ADAMS/Rail software package. In further co-simulations, five conventional control methods are compared with the proposed approach under the same conditions. Co-simulation results indicate that the new control strategy is effective in improving the safety performance of railway vehicles.

  1. Feature transformation of neural activity with sparse and low-rank decomposition

    NASA Astrophysics Data System (ADS)

    Ni, Kang-Yu; Benvenuto, James; Bhattacharyya, Rajan; Millin, Rachel

    2015-03-01

    We propose a novel application of the sparse and low-rank (SLR) decomposition method to decode cognitive states for concept activity measured using fMRI BOLD. Current decoding methods attempt to reduce the dimensionality of fMRI BOLD signals to increase classification rate, but do not address the separable issues of multiple noise sources and complexity in the underlying data. Our feature transformation method extends SLR to separate task activity from the resting state and extract concept specific cognitive state. We show a significant increase in single trial decoding of concepts from fMRI BOLD using SLR to extract task specific cognitive state.

  2. Condoms and Contexts: Profiles of Sexual Risk and Safety Among Young Heterosexually Active Men

    PubMed Central

    Masters, N. Tatiana; Casey, Erin; Beadnell, Blair; Morrison, Diane M.; Hoppe, Marilyn J.; Wells, Elizabeth A.

    2014-01-01

    Heterosexual men’s sexual safety behavior is important to controlling the U.S. epidemic of sexually transmitted infections (STIs), including HIV. While sexual safety is often treated as a single behavior, such as condom use, it can also be conceptualized as resulting from multiple factors. Doing so can help us achieve more nuanced understandings of sexual risk and safety within partner-related contexts. We used Latent Class Analysis with data collected online from 18-25 year old heterosexually active U.S. men (n = 432) to empirically derive a typology of the patterns of sexual safety strategies they employ. Indicators were sexual risk reduction strategies used in the past year with the most recent female sex partner: Condom use, discussing sexual histories, STI testing, agreeing to be monogamous, and discussing birth control. We identified four subgroups: Risk Takers (12%), Condom Reliers (25%), Multistrategists (28%), and Relationship Reliers (35%). Partner-related context factors – number of past-year sex partners, relationship commitment, and sexual concurrency – predicted subgroup membership. Findings support tailoring STI prevention to men’s sexual risk-safety subgroups. Interventions should certainly continue to encourage condom use, but should also include information on how partner-related context factors and alternate sexual safety strategies can help men reduce risk for themselves and their partners. PMID:25256019

  3. Identifying Key Features of Effective Active Learning: The Effects of Writing and Peer Discussion

    PubMed Central

    Pangle, Wiline M.; Wyatt, Kevin H.; Powell, Karli N.; Sherwood, Rachel E.

    2014-01-01

    We investigated some of the key features of effective active learning by comparing the outcomes of three different methods of implementing active-learning exercises in a majors introductory biology course. Students completed activities in one of three treatments: discussion, writing, and discussion + writing. Treatments were rotated weekly between three sections taught by three different instructors in a full factorial design. The data set was analyzed by generalized linear mixed-effect models with three independent variables: student aptitude, treatment, and instructor, and three dependent (assessment) variables: change in score on pre- and postactivity clicker questions, and coding scores on in-class writing and exam essays. All independent variables had significant effects on student performance for at least one of the dependent variables. Students with higher aptitude scored higher on all assessments. Student scores were higher on exam essay questions when the activity was implemented with a writing component compared with peer discussion only. There was a significant effect of instructor, with instructors showing different degrees of effectiveness with active-learning techniques. We suggest that individual writing should be implemented as part of active learning whenever possible and that instructors may need training and practice to become effective with active learning. PMID:25185230

  4. Identifying key features of effective active learning: the effects of writing and peer discussion.

    PubMed

    Linton, Debra L; Pangle, Wiline M; Wyatt, Kevin H; Powell, Karli N; Sherwood, Rachel E

    2014-01-01

    We investigated some of the key features of effective active learning by comparing the outcomes of three different methods of implementing active-learning exercises in a majors introductory biology course. Students completed activities in one of three treatments: discussion, writing, and discussion + writing. Treatments were rotated weekly between three sections taught by three different instructors in a full factorial design. The data set was analyzed by generalized linear mixed-effect models with three independent variables: student aptitude, treatment, and instructor, and three dependent (assessment) variables: change in score on pre- and postactivity clicker questions, and coding scores on in-class writing and exam essays. All independent variables had significant effects on student performance for at least one of the dependent variables. Students with higher aptitude scored higher on all assessments. Student scores were higher on exam essay questions when the activity was implemented with a writing component compared with peer discussion only. There was a significant effect of instructor, with instructors showing different degrees of effectiveness with active-learning techniques. We suggest that individual writing should be implemented as part of active learning whenever possible and that instructors may need training and practice to become effective with active learning.

  5. Comparison of the surface features of two wood-based activated carbons

    SciTech Connect

    Salame, I.I.; Bandosz, T.J.

    2000-02-01

    The surface features of two carbons of wood origin were compared. One sample was manufactured using phosphoric acid activation and the other using potassium hydroxide activation. To check the susceptibility to oxidation and the stability of the porous structure, the samples were oxidized with ammonium persulfate. Structural properties of carbons and their oxidized counterparts were determined using sorption of nitrogen. Surface acidity was evaluated using Boehm titration, potentiometric titration, inverse gas chromatography, and diffuse reflectance FTIR. It was demonstrated that, despite the same wood origin, the carbons significantly differ in their pore structure and surface chemistry. The carbon obtained using KOH activation is homogeneously microporous with high surface area around 2,300 m{sup 2}/g (BET). On the other hand, the carbon manufactured using phosphoric acid contains a high volume of mesopores and its surface area is significantly lower. The carbons also differ in their surface chemistry and susceptibility to oxidation.

  6. Distinctive microstructural features of aged sodium silicate-activated slag concretes

    SciTech Connect

    San Nicolas, Rackel; Bernal, Susan A.; Mejía de Gutiérrez, Ruby; Deventer, Jannie S.J. van; Provis, John L.

    2014-11-15

    Electron microscopic characterisation of 7-year old alkali-activated blast-furnace slag concretes enabled the identification of distinct microstructural features, providing insight into the mechanisms by which these materials evolve over time. Backscattered electron images show the formation of Liesegang-type ring formations, suggesting that the reaction at advanced age is likely to follow an Oswald supersaturation–nucleation–depletion cycle. Segregation of Ca-rich veins, related to the formation of Ca(OH){sub 2}, is observed in microcracked regions due to the ongoing reaction between the pore solution and available calcium from remnant slag grains. A highly dense and uniform interfacial transition zone is identified between siliceous aggregate particles and the alkali activated slag binders, across the concretes assessed. Alkali-activated slag concretes retain a highly dense and stable microstructure at advanced ages, where any microcracks induced at early ages seem to be partially closing, and the remnant slag grains continue reacting.

  7. 78 FR 18617 - Recreational Boating Safety Projects, Programs and Activities Funded Under Provisions of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-27

    ... projects related to carbon monoxide poisoning, propeller injury mitigation, and manufacturer compliance... wide spectrum of boating safety related topics and is dedicated to reducing loss of life, injuries, and... of Coast Guard expenses for personnel and activities directly related to coordinating and...

  8. 78 FR 65661 - Agency Information Collection Activities; Proposed Collection; Comment Request; Food Safety Survey

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-01

    ... HUMAN SERVICES Food and Drug Administration Agency Information Collection Activities; Proposed Collection; Comment Request; Food Safety Survey AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA or we) is announcing an opportunity for public comment...

  9. Parental and Adolescent Perceptions of Neighborhood Safety Related to Adolescents' Physical Activity in Their Neighborhood

    ERIC Educational Resources Information Center

    Esteban-Cornejo, Irene; Carlson, Jordan A.; Conway, Terry L.; Cain, Kelli L.; Saelens, Brian E.; Frank, Lawrence D.; Glanz, Karen; Roman, Caterina G.; Sallis, James F.

    2016-01-01

    Purpose: The purpose of this study was to examine the association between adolescent and parental perceptions of neighborhood safety and adolescents' physical activity in multiple locations and to investigate the moderating effect of sex within this association. Method: This cross-sectional study was conducted with 928 adolescents aged 12 to 16…

  10. 78 FR 10261 - Pipeline Safety: Information Collection Activities, Revision to Gas Distribution Annual Report

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-13

    ... Activities, Revision to Gas Distribution Annual Report AGENCY: Pipeline and Hazardous Materials Safety... Office of Management and Budget (OMB) approval for the revision of the gas distribution annual report... Privacy Act Statement in the Federal Register published on April 11, 2000, (65 FR 19477) or visit...

  11. T & I--Building Construction, Safety. Kit No. 1. Instructor's Manual [and] Student Learning Activity Guide.

    ERIC Educational Resources Information Center

    Howard, John

    An instructor's manual and student activity guide on building construction safety are provided in this set of prevocational education materials which focuses on the vocational area of trade and industry. (This set of materials is one of ninety-two prevocational education sets arranged around a cluster of seven vocational offerings: agriculture,…

  12. Music-induced emotions can be predicted from a combination of brain activity and acoustic features.

    PubMed

    Daly, Ian; Williams, Duncan; Hallowell, James; Hwang, Faustina; Kirke, Alexis; Malik, Asad; Weaver, James; Miranda, Eduardo; Nasuto, Slawomir J

    2015-12-01

    It is widely acknowledged that music can communicate and induce a wide range of emotions in the listener. However, music is a highly-complex audio signal composed of a wide range of complex time- and frequency-varying components. Additionally, music-induced emotions are known to differ greatly between listeners. Therefore, it is not immediately clear what emotions will be induced in a given individual by a piece of music. We attempt to predict the music-induced emotional response in a listener by measuring the activity in the listeners electroencephalogram (EEG). We combine these measures with acoustic descriptors of the music, an approach that allows us to consider music as a complex set of time-varying acoustic features, independently of any specific music theory. Regression models are found which allow us to predict the music-induced emotions of our participants with a correlation between the actual and predicted responses of up to r=0.234,p<0.001. This regression fit suggests that over 20% of the variance of the participant's music induced emotions can be predicted by their neural activity and the properties of the music. Given the large amount of noise, non-stationarity, and non-linearity in both EEG and music, this is an encouraging result. Additionally, the combination of measures of brain activity and acoustic features describing the music played to our participants allows us to predict music-induced emotions with significantly higher accuracies than either feature type alone (p<0.01). PMID:26544602

  13. The analysis of solar activity features by means of the BASIS digital imaging system

    NASA Astrophysics Data System (ADS)

    Messerotti, M.; Lampi, L.; Furlani, S.; Zlobec, P.

    CCD-based digital imaging systems are powerful tools for the analysis of solar activity features in real-time or as post-processing. Despite the actual sensor-limited resolutions of low-cost systems, interesting projects can be carried out such as, for instance, the tracing of photospheric motions, in principle also in automatic mode. With regard to that BASIS, a digital imaging system for the sun operated at Trieste, will be briefly described. Possible applications as mentioned above will be discussed with emphasis on photospheric and chromospheric patterns.

  14. Annual Report To Congress. Department of Energy Activities Relating to the Defense Nuclear Facilities Safety Board, Calendar Year 2003

    SciTech Connect

    None, None

    2004-02-28

    The Department of Energy (Department) submits an Annual Report to Congress each year detailing the Department’s activities relating to the Defense Nuclear Facilities Safety Board (Board), which provides advice and recommendations to the Secretary of Energy (Secretary) regarding public health and safety issues at the Department’s defense nuclear facilities. In 2003, the Department continued ongoing activities to resolve issues identified by the Board in formal recommendations and correspondence, staff issue reports pertaining to Department facilities, and public meetings and briefings. Additionally, the Department is implementing several key safety initiatives to address and prevent safety issues: safety culture and review of the Columbia accident investigation; risk reduction through stabilization of excess nuclear materials; the Facility Representative Program; independent oversight and performance assurance; the Federal Technical Capability Program (FTCP); executive safety initiatives; and quality assurance activities. The following summarizes the key activities addressed in this Annual Report.

  15. The Activities of the International Criticality Safety Benchmark Evaluation Project (ICSBEP)

    SciTech Connect

    Briggs, Joseph Blair

    2001-10-01

    The International Criticality Safety Benchmark Evaluation Project (ICSBEP) was initiated in 1992 by the United States Department of Energy. The ICSBEP became an official activity of the Organization for Economic Cooperation and Development (OECD) – Nuclear Energy Agency (NEA) in 1995. Representatives from the United States, United Kingdom, France, Japan, the Russian Federation, Hungary, Republic of Korea, Slovenia, Yugoslavia, Kazakhstan, Spain, and Israel are now participating. The purpose of the ICSBEP is to identify, evaluate, verify, and formally document a comprehensive and internationally peer-reviewed set of criticality safety benchmark data. The work of the ICSBEP is published as an OECD handbook entitled “International Handbook of Evaluated Criticality Safety Benchmark Experiments”. The 2001 Edition of the Handbook contains benchmark specifications for 2642 critical or subcritical configurations that are intended for use in validation efforts and for testing basic nuclear data.

  16. Concepts and techniques: Active electronics and computers in safety-critical accelerator operation

    SciTech Connect

    Frankel, R.S.

    1995-12-31

    The Relativistic Heavy Ion Collider (RHIC) under construction at Brookhaven National Laboratory, requires an extensive Access Control System to protect personnel from Radiation, Oxygen Deficiency and Electrical hazards. In addition, the complicated nature of operation of the Collider as part of a complex of other Accelerators necessitates the use of active electronic measurement circuitry to ensure compliance with established Operational Safety Limits. Solutions were devised which permit the use of modern computer and interconnections technology for Safety-Critical applications, while preserving and enhancing, tried and proven protection methods. In addition a set of Guidelines, regarding required performance for Accelerator Safety Systems and a Handbook of design criteria and rules were developed to assist future system designers and to provide a framework for internal review and regulation.

  17. Joint spatial-spectral feature space clustering for speech activity detection from ECoG signals.

    PubMed

    Kanas, Vasileios G; Mporas, Iosif; Benz, Heather L; Sgarbas, Kyriakos N; Bezerianos, Anastasios; Crone, Nathan E

    2014-04-01

    Brain-machine interfaces for speech restoration have been extensively studied for more than two decades. The success of such a system will depend in part on selecting the best brain recording sites and signal features corresponding to speech production. The purpose of this study was to detect speech activity automatically from electrocorticographic signals based on joint spatial-frequency clustering of the ECoG feature space. For this study, the ECoG signals were recorded while a subject performed two different syllable repetition tasks. We found that the optimal frequency resolution to detect speech activity from ECoG signals was 8 Hz, achieving 98.8% accuracy by employing support vector machines as a classifier. We also defined the cortical areas that held the most information about the discrimination of speech and nonspeech time intervals. Additionally, the results shed light on the distinct cortical areas associated with the two syllables repetition tasks and may contribute to the development of portable ECoG-based communication.

  18. The features of longitudinal distribution of solar spots during the last 13 solar activity minima

    NASA Astrophysics Data System (ADS)

    Kostuchenko, I. G.; Benevolenskaya, E. E.

    2015-12-01

    We analyzed the features of the longitudinal distribution of the areas of solar spots during the solar activity minima, from the 11th cycle to the last minimum, based on data provided by the Greenwich Observatory and the Marshall Research Center. We discovered that the solar spots evolved in one or two neighboring bands (in terms of longitude), the Carrington longitude of which smoothly displaced from the east to the west, in the phase of the deep minimum in all of the considered cases. The spots at the high latitudes associated with a "new" cycle evolved on the same longitude bands. All of this led to the noticeable longitudinal asymmetry of magnetic fluxes related to the spots and flocculi. Based on our research, we propose the hypothesis that a nonaxisymmetric component of the total magnetic flux of the Sun is generated, together with the dipole component, by the solar dynamo mechanism, which is a typical feature of the phase of a minimum between the solar activity cycles.

  19. Genomic features of uncultured methylotrophs in activated-sludge microbiomes grown under different enrichment procedures.

    PubMed

    Fujinawa, Kazuki; Asai, Yusuke; Miyahara, Morio; Kouzuma, Atsushi; Abe, Takashi; Watanabe, Kazuya

    2016-01-01

    Methylotrophs are organisms that are able to grow on C1 compounds as carbon and energy sources. They play important roles in the global carbon cycle and contribute largely to industrial wastewater treatment. To identify and characterize methylotrophs that are involved in methanol degradation in wastewater-treatment plants, methanol-fed activated-sludge (MAS) microbiomes were subjected to phylogenetic and metagenomic analyses, and genomic features of dominant methylotrophs in MAS were compared with those preferentially grown in laboratory enrichment cultures (LECs). These analyses consistently indicate that Hyphomicrobium plays important roles in MAS, while Methylophilus occurred predominantly in LECs. Comparative analyses of bin genomes reconstructed for the Hyphomicrobium and Methylophilus methylotrophs suggest that they have different C1-assimilation pathways. In addition, function-module analyses suggest that their cell-surface structures are different. Comparison of the MAS bin genome with genomes of closely related Hyphomicrobium isolates suggests that genes unnecessary in MAS (for instance, genes for anaerobic respiration) have been lost from the genome of the dominant methylotroph. We suggest that genomic features and coded functions in the MAS bin genome provide us with insights into how this methylotroph adapts to activated-sludge ecosystems. PMID:27221669

  20. Genomic features of uncultured methylotrophs in activated-sludge microbiomes grown under different enrichment procedures

    PubMed Central

    Fujinawa, Kazuki; Asai, Yusuke; Miyahara, Morio; Kouzuma, Atsushi; Abe, Takashi; Watanabe, Kazuya

    2016-01-01

    Methylotrophs are organisms that are able to grow on C1 compounds as carbon and energy sources. They play important roles in the global carbon cycle and contribute largely to industrial wastewater treatment. To identify and characterize methylotrophs that are involved in methanol degradation in wastewater-treatment plants, methanol-fed activated-sludge (MAS) microbiomes were subjected to phylogenetic and metagenomic analyses, and genomic features of dominant methylotrophs in MAS were compared with those preferentially grown in laboratory enrichment cultures (LECs). These analyses consistently indicate that Hyphomicrobium plays important roles in MAS, while Methylophilus occurred predominantly in LECs. Comparative analyses of bin genomes reconstructed for the Hyphomicrobium and Methylophilus methylotrophs suggest that they have different C1-assimilation pathways. In addition, function-module analyses suggest that their cell-surface structures are different. Comparison of the MAS bin genome with genomes of closely related Hyphomicrobium isolates suggests that genes unnecessary in MAS (for instance, genes for anaerobic respiration) have been lost from the genome of the dominant methylotroph. We suggest that genomic features and coded functions in the MAS bin genome provide us with insights into how this methylotroph adapts to activated-sludge ecosystems. PMID:27221669

  1. The safety attitudes of people who use multi-purpose recreation facilities as a physical activity setting.

    PubMed

    Finch, Caroline F; Otago, Leonie; White, Peta; Donaldson, Alex; Mahoney, Mary

    2011-06-01

    Multi-purpose recreation facilities (MPRFs) are a popular setting for physical activity and it is therefore important that they are safe for all patrons. However, the attitudes of MPRF users towards safety are a potential barrier to the success of injury prevention programmes implemented within MPRFs. This article reports a survey of the safety attitudes of over 700 users of four indoor MPRFs. Factor analysis of 12 five-point Likert scale statements showed that the attitudes clustered around three major dimensions - the importance of safety, the benefits of safety and the perceptions of injury risk. Together, these three dimensions accounted for 49% of the variability in the attitudes. More than 85% of respondents agreed/strongly agreed that: safety was an important aspect of physical activity participation; being injured affected enjoyment of physical activity; people should adopt appropriate safety measures for all physical activity; and individuals were responsible for their own safety. The MPRF users, particularly women and older people, were generally safety conscious, believed in adopting safety measures, and were willing to take responsibility for their own safety. Facility managers can be confident that if they provide evidence-based injury prevention interventions in these settings, then users will respond appropriately and adopt the promoted behaviours.

  2. Development and Testing of a Nutrition, Food Safety, and Physical Activity Checklist for EFNEP and FSNE Adult Programs

    ERIC Educational Resources Information Center

    Bradford, Traliece; Serrano, Elena L.; Cox, Ruby H.; Lambur, Michael

    2010-01-01

    Objective: To develop and assess reliability and validity of the Nutrition, Food Safety, and Physical Activity Checklist to measure nutrition, food safety, and physical activity practices among adult Expanded Food and Nutrition Education Program (EFNEP) and Food Stamp Nutrition Education program (FSNE) participants. Methods: Test-retest…

  3. Neighborhood Crime-Related Safety and Its Relation to Children's Physical Activity.

    PubMed

    Kneeshaw-Price, Stephanie H; Saelens, Brian E; Sallis, James F; Frank, Lawrence D; Grembowski, David E; Hannon, Peggy A; Smith, Nicholas L; Chan, K C Gary

    2015-06-01

    Crime is both a societal safety and public health issue. Examining different measures and aspects of crime-related safety and their correlations may provide insight into the unclear relationship between crime and children's physical activity. We evaluated five neighborhood crime-related safety measures to determine how they were interrelated. We then explored which crime-related safety measures were associated with children's total moderate-to-vigorous physical activity (MVPA) and MVPA in their neighborhoods. Significant positive correlations between observed neighborhood incivilities and parents' perceptions of general crime and disorder were found (r = 0.30, p = 0.0002), as were associations between parents' perceptions of general crime and disorder and perceptions of stranger danger (r = 0.30, p = 0.0002). Parent report of prior crime victimization in their neighborhood was associated with observed neighborhood incivilities (r = 0.22, p = 0.007) and their perceptions of both stranger danger (r = 0.24, p = 0.003) and general crime and disorder (r = 0.37, p < 0.0001). After accounting for covariates, police-reported crime within the census block group in which children lived was associated with less physical activity, both total and in their neighborhood (beta = -0.09, p = 0.005, beta = -0.01, p = 0.02, respectively). Neighborhood-active children living in the lowest crime-quartile neighborhoods based on police reports had 40 min more of total MVPA on average compared to neighborhood-active children living in the highest crime-quartile neighborhoods. Findings suggest that police reports of neighborhood crime may be contributing to lower children's physical activity.

  4. A national survey of sun safety activities at U.S. zoos.

    PubMed

    Talosig, M A; Mayer, J A; Eckhardt, L; Lewis, E C; Kwon, H; Belch, G E; Eichenfield, L F; Elder, J P; Engelberg, M

    2000-08-01

    Skin cancer incidence in the United States has increased. Exposure to ultraviolet radiation (UVR) is a major risk factor for skin cancer. Every year, millions of children with their families visit outdoor leisure facilities such as zoos where overexposure to UVR may occur. This study was conducted to assess the proportion of U.S. zoos that provided sun safety activities during the summer of 1998 and their willingness to incorporate skin cancer prevention strategies at their facility in the future. A 56 item self-administered questionnaire was mailed to 140 zoos accredited by the American Zoo and Aquarium Association. A follow-up telephone survey was also conducted with 33 mail non-respondents. The response rate was 97% (N = 136). Results revealed that in the summer of 1998, only 5% of zoos provided sun safety recommendations to visitors and 25% of zoos provided recommendations to their employees. The recommendations made most often to visitors and employees were to use sunscreen and wear protective clothing. Eighty-five percent of zoos indicated interest in providing sun safety activities at their facilities in the future. The next step will be to design environmentally appropriate sun safety programs for zoos and to encourage zoos to implement these programs.

  5. Causation mechanisms in car-to-vulnerable road user crashes: implications for active safety systems.

    PubMed

    Habibovic, Azra; Davidsson, Johan

    2012-11-01

    Vulnerable road users (VRUs), such as pedestrians and bicyclists, are often involved in crashes with passenger cars. One way to prevent these crashes is to deploy active safety systems that support the car drivers and/or VRUs. However, to develop such systems, a thorough understanding of crash causation mechanisms is required. The aim of this study is to identify crash causation mechanisms from the perspective of the VRUs, and to explore the implications of these mechanisms for the development of active safety systems. Data originate from the European project SafetyNet, where 995 crashes were in-depth investigated using the SafetyNet Accident Causation System (SNACS). To limit the scope, this study analyzed only intersection crashes involving VRUs. A total of 56 VRU crashes were aggregated. Results suggest that, while 30% of the VRUs did not see the conflict car due to visual obstructions in the traffic environment, 70% of the VRUs saw the car before the collision, but still misunderstood the traffic situation and/or made an inadequate plan of action. An important implication that follows from this is that, while detection of cars is clearly an issue that needs to be addressed, it is even more important to help the VRUs to correctly understand traffic situation (e.g., does the driver intend to slow down, and if s/he does, is it to let the VRU cross or for some other reason?). The former issue suggests a role for various cooperative active safety systems, as the obstacles are generally impenetrable with regular sensors. The latter issue is less straightforward. While various systems can be proposed, such as providing gap size estimation and reducing the car speed variability, the functional merits of each such a system need to be further investigated. PMID:23036427

  6. Relationship between the latest activity of mare volcanism and topographic features of the Moon

    NASA Astrophysics Data System (ADS)

    Kato, Shinsuke; Morota, Tomokatsu; Yamaguchi, Yasushi; Watanabe, Sei-ichiro; Otake, Hisashi; Ohtake, Makiko

    2016-04-01

    Lunar mare basalts provide insights into compositions and thermal history of lunar mantle. According to crater counting analysis with remote sensing data, the model ages of mare basalt units indicate a second peak of magma activity at the end of mare volcanism (~2 Ga), and the latest eruptions were limited in the Procellarum KREEP Terrane (PKT), which has high abundances of heat-producing elements. In order to understand the mechanism for causing the second peak and its magma source, we examined the correlation between the titanium contents and eruption ages of mare basalt units using compositional and chronological data updated by SELENE/Kaguya. Although no systematic relationship is observed globally, a rapid increase in mean titanium (Ti) content occurred at 2.3 Ga in the PKT, suggesting that the magma source of mare basalts changed at that time. The high-Ti basaltic eruption, which occurred at the late stage of mare volcanism, can be correlated with the second peak of volcanic activity at ~2 Ga. The latest volcanic activity can be explained by a high-Ti hot plume originated from the core-mantle boundary. If the hot plume was occurred, the topographic features formed by the hot plume may be remained. We calculated the difference between topography and selenoid and found the circular feature like a plateau in the center of the PKT, which scale is ~1000 km horizontal and ~500 m vertical. We investigated the timing of ridge formation in the PKT by using stratigraphic relationship between mare basalts and ridges. The ridges were formed before and after the high-Ti basaltic eruptions and seem to be along with the plateau. These results suggest that the plateau formation is connected with the high-Ti basaltic eruptions.

  7. Efficacy and Safety of a Hyaluronic Acid Filler to Correct Aesthetically Detracting or Deficient Features of the Asian Nose: A Prospective, Open-Label, Long-Term Study

    PubMed Central

    Liew, Steven; Scamp, Terrence; de Maio, Mauricio; Halstead, Michael; Johnston, Nicole; Silberberg, Michael; Rogers, John D.

    2016-01-01

    Background There is increasing interest among patients and plastic surgeons for alternatives to rhinoplasty, a common surgical procedure performed in Asia. Objectives To evaluate the safety, efficacy, and longevity of a hyaluronic acid filler in the correction of aesthetically detracting or deficient features of the Asian nose. Methods Twenty-nine carefully screened Asian patients had their noses corrected with the study filler (Juvéderm VOLUMA [Allergan plc, Dublin, Ireland] with lidocaine injectable gel), reflecting individualized treatment goals and utilizing a standardized injection procedure, and were followed for over 12 months. Results A clinically meaningful correction (≥1 grade improvement on the Assessment of Aesthetic Improvement Scale) was achieved in 27 (93.1%) patients at the first follow-up visit. This was maintained in 28 (96.6%) patients at the final visit, based on the independent assessments of a central non-injecting physician and the patients. At this final visit, 23 (79.3%) patients were satisfied or very satisfied with the study filler and 25 (86.2%) would recommend it to others. In this small series of patients, there were no serious adverse events (AEs), with all treatment-related AEs being mild to moderate, transient injection site reactions, unrelated to the study filler. Conclusions Using specific eligibility criteria, individualized treatment goals, and a standardized injection procedure, the study filler corrected aesthetically detracting or deficient features of the Asian nose, with the therapeutic effects lasting for over 12 months, consistent with a high degree of patient satisfaction. This study supports the safety and efficacy of this HA filler for specific nose augmentation procedures in selected Asian patients. Level of Evidence: 3 Therapeutic PMID:27301371

  8. Frequencies, Laboratory Features, and Granulocyte Activation in Chinese Patients with CALR-Mutated Myeloproliferative Neoplasms

    PubMed Central

    Tian, Ruiyuan; Chang, Jianmei; Li, Jianlan; Tan, Yanhong; Xu, Zhifang; Ren, Fanggang; Zhao, Junxia; Pan, Jie; Zhang, Na; Wang, Xiaojuan; He, Jianxia; Yang, Wanfang; Wang, Hongwei

    2015-01-01

    Somatic mutations in the CALR gene have been recently identified as acquired alterations in myeloproliferative neoplasms (MPNs). In this study, we evaluated mutation frequencies, laboratory features, and granulocyte activation in Chinese patients with MPNs. A combination of qualitative allele-specific polymerase chain reaction and Sanger sequencing was used to detect three driver mutations (i.e., CALR, JAK2V617F, and MPL). CALR mutations were identified in 8.4% of cases with essential thrombocythemia (ET) and 5.3% of cases with primary myelofibrosis (PMF). Moreover, 25% of polycythemia vera, 29.5% of ET, and 48.1% of PMF were negative for all three mutations (JAK2V617F, MPL, and CALR). Compared with those patients with JAK2V617F mutation, CALR-mutated ET patients displayed unique hematological phenotypes, including higher platelet counts, and lower leukocyte counts and hemoglobin levels. Significant differences were not found between Chinese PMF patients with mutants CALR and JAK2V617F in terms of laboratory features. Interestingly, patients with CALR mutations showed markedly decreased levels of leukocyte alkaline phosphatase (LAP) expression, whereas those with JAK2V617F mutation presented with elevated levels. Overall, a lower mutant rate of CALR gene and a higher triple-negative rate were identified in the cohort of Chinese patients with MPNs. This result indicates that an undiscovered mutant gene may have a significant role in these patients. Moreover, these pathological features further imply that the disease biology varies considerably between mutants CALR and JAK2V617F. PMID:26375990

  9. Microscopic and mesoscopic structural features of an activated carbon sample, prepared from sorghum via activation by phosphoric acid

    SciTech Connect

    Temleitner, László; Pusztai, László; Rubio-Arroyo, Manuel F.; Aguilar-López, Sergio; Pizio, Orest

    2012-12-15

    Graphical abstract: Display Omitted Highlights: ► Preparation of a new activated carbon sample from sorghum. ► Characterization by adsorption/desorption methods. ► Determination of the structure by synchrotron X-ray diffraction. ► The sample is amorphous and contains distorted graphene fragments. ► A characteristic nanoscale distance is established from the radial distribution function. -- Abstract: An acidic chemical activation procedure has been used for preparing activated carbon with a surface area exceeding 1000 m{sup 2}/g from sorghum. In order to reveal structural features, synchrotron X-ray diffraction measurements have been performed. The structure of the material has been characterized by the total scattering structure factor and the radial distribution function describing short-range arrangement of atoms at distances of the order of a few atomic diameters as well as correlations at a longer scale, of the order of nanometers. The atomic arrangement has been found to be consistent with that of amorphous graphite-like carbon. As far as the mesoscopic structure is concerned, the presence of a characteristic distance is suggested on the basis of the clear nanometer scale oscillations of the radial distribution function, which distance may be assigned as the mesopore size in the material. It is suggested that the approach devized here may later be applied routinely for other activated carbon samples, too, for characterizing atomic and nanoscale order simultaneously.

  10. The neural organization of semantic memory: Electrophysiological activity suggests feature-based segregation

    PubMed Central

    Sitnikova, Tatiana; West, W. Caroline; Kuperberg, Gina R.; Holcomb, Phillip J.

    2007-01-01

    Despite decades of research, it remains controversial whether semantic knowledge is anatomically segregated in the human brain. To address this question, we recorded event-related potentials (ERPs) while participants viewed pictures of animals and tools. Within the 200–600-ms epoch after stimulus presentation, animals (relative to tools) elicited an increased anterior negativity that, based on previous ERP studies, we interpret as associated with semantic processing of visual object attributes. In contrast, tools (relative to animals) evoked an enhanced posterior left-lateralized negativity that, according to prior research, might reflect accessing knowledge of characteristic motion and/or more general functional properties of objects. These results support the hypothesis of the neuroanatomical knowledge organization at the level of object features: the observed neurophysiological activity was modulated by the features that were most salient for object recognition. The high temporal resolution of ERPs allowed us to demonstrate that differences in processing animals and tools occurred specifically within the time-window encompassing semantic analysis. PMID:16129544

  11. OCCUPATIONAL SAFETY AND HEALTH IN DISASTER RESTORATION ACTIVITY AFTER SOME MAJOR EARTHQUAKES

    NASA Astrophysics Data System (ADS)

    Toyosawa, Yasuo; Itoh, Kazuya; Kikkawa, Naotaka

    Occupational safety and health in disaster restoration activity following the Great Hanshin Earthquake (1995), Niigata Chuetsu Earthquake (2004), Niigata Chuetsu-oki Earthquake (2007) Great East Japan Earthquake (2011) were analyzed and characterized in order to raise awareness on the risks and hazards in such work. In this scenario, the predominant type of accident is a "fall" which increases mainly due to the fact that labourers are working to repair houses and buildings. On the other hand, landslides were prevalent in the Niigata Chuetsu Earthquake, resulting in more accidents occurring during geotechnical works rather than in buildings construction works. In the abnormal conditions that characterize recovery activities, when safety and health measures have a tendency to be neglected, it is important to reinstate adequate measures as soon as possible by carrying out the usial risk assessments.

  12. Features of the electronic structure of the active center of an HbS molecule

    NASA Astrophysics Data System (ADS)

    Novoselov, D. Yu.; Korotin, Dm. M.; Anisimov, V. I.

    2016-01-01

    Features of the electronic structure of the nonprotein part of the mutant form of the human hemoglobin molecule, HbS, are studied along with the magnetic state of the iron ion that is the "nucleus" of the active center of the molecule. It is found that the mutant form of the HbS molecule differs from a normal hemoglobin molecule by the distortion of the local environment of the iron ion, which changes the energy level splitting by a crystal field. As a result of ab initio calculations, the magnetic transition in the iron atom from the high-spin state to the low-spin state upon the addition of molecular oxygen to hemoglobin molecule is reproduced. It is established for the first time that a change in the crystal and electronic structure of the active center as a result of a mutation can lead to a substantial change in the energy of the bond between the active center of the hemoglobin molecule and an oxygen molecule.

  13. Image sensor innovations for low light levels with active imaging features

    NASA Astrophysics Data System (ADS)

    Powell, Gareth H.; Fereyre, Pierre

    2015-02-01

    Advances in CMOS imaging enable image capture at lower light levels. Color detection is also possible where human vision becomes less sensitive in night conditions. In daytime conditions, there are a number of climatic conditions such as rain, fog, snow or smoke etc. that render traditional `intelligent' outdoor cameras that perform various forms of detection and identification tasks relatively ineffective. It has been proven that an adapted five transistor pixel CMOS sensor can perform range-gated active imaging that extends considerably the usability of intelligent cameras in the most difficult conditions. This paper discusses advanced state of the art image sensors with embedded features, with emphasis on the everimportant size, weight, power and cost benefits and discusses the new applications that are enabled.

  14. Feature selection for wearable smartphone-based human activity recognition with able bodied, elderly, and stroke patients.

    PubMed

    Capela, Nicole A; Lemaire, Edward D; Baddour, Natalie

    2015-01-01

    Human activity recognition (HAR), using wearable sensors, is a growing area with the potential to provide valuable information on patient mobility to rehabilitation specialists. Smartphones with accelerometer and gyroscope sensors are a convenient, minimally invasive, and low cost approach for mobility monitoring. HAR systems typically pre-process raw signals, segment the signals, and then extract features to be used in a classifier. Feature selection is a crucial step in the process to reduce potentially large data dimensionality and provide viable parameters to enable activity classification. Most HAR systems are customized to an individual research group, including a unique data set, classes, algorithms, and signal features. These data sets are obtained predominantly from able-bodied participants. In this paper, smartphone accelerometer and gyroscope sensor data were collected from populations that can benefit from human activity recognition: able-bodied, elderly, and stroke patients. Data from a consecutive sequence of 41 mobility tasks (18 different tasks) were collected for a total of 44 participants. Seventy-six signal features were calculated and subsets of these features were selected using three filter-based, classifier-independent, feature selection methods (Relief-F, Correlation-based Feature Selection, Fast Correlation Based Filter). The feature subsets were then evaluated using three generic classifiers (Naïve Bayes, Support Vector Machine, j48 Decision Tree). Common features were identified for all three populations, although the stroke population subset had some differences from both able-bodied and elderly sets. Evaluation with the three classifiers showed that the feature subsets produced similar or better accuracies than classification with the entire feature set. Therefore, since these feature subsets are classifier-independent, they should be useful for developing and improving HAR systems across and within populations.

  15. Feature selection for wearable smartphone-based human activity recognition with able bodied, elderly, and stroke patients.

    PubMed

    Capela, Nicole A; Lemaire, Edward D; Baddour, Natalie

    2015-01-01

    Human activity recognition (HAR), using wearable sensors, is a growing area with the potential to provide valuable information on patient mobility to rehabilitation specialists. Smartphones with accelerometer and gyroscope sensors are a convenient, minimally invasive, and low cost approach for mobility monitoring. HAR systems typically pre-process raw signals, segment the signals, and then extract features to be used in a classifier. Feature selection is a crucial step in the process to reduce potentially large data dimensionality and provide viable parameters to enable activity classification. Most HAR systems are customized to an individual research group, including a unique data set, classes, algorithms, and signal features. These data sets are obtained predominantly from able-bodied participants. In this paper, smartphone accelerometer and gyroscope sensor data were collected from populations that can benefit from human activity recognition: able-bodied, elderly, and stroke patients. Data from a consecutive sequence of 41 mobility tasks (18 different tasks) were collected for a total of 44 participants. Seventy-six signal features were calculated and subsets of these features were selected using three filter-based, classifier-independent, feature selection methods (Relief-F, Correlation-based Feature Selection, Fast Correlation Based Filter). The feature subsets were then evaluated using three generic classifiers (Naïve Bayes, Support Vector Machine, j48 Decision Tree). Common features were identified for all three populations, although the stroke population subset had some differences from both able-bodied and elderly sets. Evaluation with the three classifiers showed that the feature subsets produced similar or better accuracies than classification with the entire feature set. Therefore, since these feature subsets are classifier-independent, they should be useful for developing and improving HAR systems across and within populations. PMID:25885272

  16. Validation of a common data model for active safety surveillance research

    PubMed Central

    Ryan, Patrick B; Reich, Christian G; Hartzema, Abraham G; Stang, Paul E

    2011-01-01

    Objective Systematic analysis of observational medical databases for active safety surveillance is hindered by the variation in data models and coding systems. Data analysts often find robust clinical data models difficult to understand and ill suited to support their analytic approaches. Further, some models do not facilitate the computations required for systematic analysis across many interventions and outcomes for large datasets. Translating the data from these idiosyncratic data models to a common data model (CDM) could facilitate both the analysts' understanding and the suitability for large-scale systematic analysis. In addition to facilitating analysis, a suitable CDM has to faithfully represent the source observational database. Before beginning to use the Observational Medical Outcomes Partnership (OMOP) CDM and a related dictionary of standardized terminologies for a study of large-scale systematic active safety surveillance, the authors validated the model's suitability for this use by example. Validation by example To validate the OMOP CDM, the model was instantiated into a relational database, data from 10 different observational healthcare databases were loaded into separate instances, a comprehensive array of analytic methods that operate on the data model was created, and these methods were executed against the databases to measure performance. Conclusion There was acceptable representation of the data from 10 observational databases in the OMOP CDM using the standardized terminologies selected, and a range of analytic methods was developed and executed with sufficient performance to be useful for active safety surveillance. PMID:22037893

  17. Is the relationship between the built environment and physical activity moderated by perceptions of crime and safety?

    PubMed Central

    2014-01-01

    Background Direct relationships between safety concerns and physical activity have been inconsistently patterned in the literature. To tease out these relationships, crime, pedestrian, and traffic safety were examined as moderators of built environment associations with physical activity. Methods Exploratory analyses used two cross-sectional studies of 2068 adults ages 20–65 and 718 seniors ages 66+ with similar designs and measures. The studies were conducted in the Baltimore, Maryland-Washington, DC and Seattle-King County, Washington regions during 2001–2005 (adults) and 2005–2008 (seniors). Participants were recruited from areas selected to sample high- and low- income and walkability. Independent variables perceived crime, traffic, and pedestrian safety were measured using scales from validated instruments. A GIS-based walkability index was calculated for a street-network buffer around each participant’s home address. Outcomes were total physical activity measured using accelerometers and transportation and leisure walking measured with validated self-reports (IPAQ-long). Mixed effects regression models were conducted separately for each sample. Results Of 36 interactions evaluated across both studies, only 5 were significant (p < .05). Significant interactions did not consistently support a pattern of highest physical activity when safety was rated high and environments were favorable. There was not consistent evidence that safety concerns reduced the beneficial effects of favorable environments on physical activity. Only pedestrian safety showed evidence of a consistent main effect with physical activity outcomes, possibly because pedestrian safety items (e.g., crosswalks, sidewalks) were not as subjective as those on the crime and traffic safety scales. Conclusions Clear relationships between crime, pedestrian, and traffic safety with physical activity levels remain elusive. The development of more precise safety variables and the use of

  18. The Activity Chain Safety and Liveness Specification of Composite Web Services

    NASA Astrophysics Data System (ADS)

    Chen, Bo; Huang, Xiaomei

    Web service composition is most impressing method for development and deployment of e-business. Description and modeling the behavior requirements of composite Web services for users and verifying composite Web service compliance to specific requirements is an important key in design of services. But most work does not address the issue of how to model the requirements that the BPEL4WS processes are supposed to satisfy. The specifications in verification works are general temporal relation based on activity or scenario in essence. Distinguish with these work, we propose a novel concept of behavior specification based on activity chain in which granularity is between activity and scenario. Chain existence mode, chain absence mode are designed to express such behavioral requirements based on activity chain that is similar with safety or liveness specification based on activity respectively. Encode them on Labeled Transition System LTS and then give them exact operation semantics. Finally, an example is illustrated.

  19. Combining EEG Microstates with fMRI Structural Features for Modeling Brain Activity.

    PubMed

    Michalopoulos, Kostas; Bourbakis, Nikolaos

    2015-12-01

    Combining information from Electroencephalography (EEG) and Functional Magnetic Resonance Imaging (fMRI) has been a topic of increased interest recently. The main advantage of the EEG is its high temporal resolution, in the scale of milliseconds, while the main advantage of fMRI is the detection of functional activity with good spatial resolution. The advantages of each modality seem to complement each other, providing better insight in the neuronal activity of the brain. The main goal of combining information from both modalities is to increase the spatial and the temporal localization of the underlying neuronal activity captured by each modality. This paper presents a novel technique based on the combination of these two modalities (EEG, fMRI) that allow a better representation and understanding of brain activities in time. EEG is modeled as a sequence of topographies, based on the notion of microstates. Hidden Markov Models (HMMs) were used to model the temporal evolution of the topography of the average Event Related Potential (ERP). For each model the Fisher score of the sequence is calculated by taking the gradient of the trained model parameters. The Fisher score describes how this sequence deviates from the learned HMM. Canonical Partial Least Squares (CPLS) were used to decompose the two datasets and fuse the EEG and fMRI features. In order to test the effectiveness of this method, the results of this methodology were compared with the results of CPLS using the average ERP signal of a single channel. The presented methodology was able to derive components that co-vary between EEG and fMRI and present significant differences between the two tasks.

  20. Combining EEG Microstates with fMRI Structural Features for Modeling Brain Activity.

    PubMed

    Michalopoulos, Kostas; Bourbakis, Nikolaos

    2015-12-01

    Combining information from Electroencephalography (EEG) and Functional Magnetic Resonance Imaging (fMRI) has been a topic of increased interest recently. The main advantage of the EEG is its high temporal resolution, in the scale of milliseconds, while the main advantage of fMRI is the detection of functional activity with good spatial resolution. The advantages of each modality seem to complement each other, providing better insight in the neuronal activity of the brain. The main goal of combining information from both modalities is to increase the spatial and the temporal localization of the underlying neuronal activity captured by each modality. This paper presents a novel technique based on the combination of these two modalities (EEG, fMRI) that allow a better representation and understanding of brain activities in time. EEG is modeled as a sequence of topographies, based on the notion of microstates. Hidden Markov Models (HMMs) were used to model the temporal evolution of the topography of the average Event Related Potential (ERP). For each model the Fisher score of the sequence is calculated by taking the gradient of the trained model parameters. The Fisher score describes how this sequence deviates from the learned HMM. Canonical Partial Least Squares (CPLS) were used to decompose the two datasets and fuse the EEG and fMRI features. In order to test the effectiveness of this method, the results of this methodology were compared with the results of CPLS using the average ERP signal of a single channel. The presented methodology was able to derive components that co-vary between EEG and fMRI and present significant differences between the two tasks. PMID:26584584

  1. Premature skin aging features rescued by inhibition of NADPH oxidase activity in XPC-deficient mice.

    PubMed

    Hosseini, Mohsen; Mahfouf, Walid; Serrano-Sanchez, Martin; Raad, Houssam; Harfouche, Ghida; Bonneu, Marc; Claverol, Stephane; Mazurier, Frederic; Rossignol, Rodrigue; Taieb, Alain; Rezvani, Hamid Reza

    2015-04-01

    Xeroderma pigmentosum type C (XP-C) is characterized mostly by a predisposition to skin cancers and accelerated photoaging, but little is known about premature skin aging in this disease. By comparing young and old mice, we found that the level of progerin and p16(INK4a) expression, β-galactosidase activity, and reactive oxygen species, which increase with age, were higher in young Xpc(-/-) mice than in young Xpc(+/+) ones. The expression level of mitochondrial complexes and mitochondrial functions in the skin of young Xpc(-/-) was as low as in control aged Xpc(+/+)animals. Furthermore, the metabolic profile in young Xpc(-/-) mice resembled that found in aged Xpc(+/+) mice. Furthermore, premature skin aging features in young Xpc(-/-) mice were mostly rescued by inhibition of nicotinamide adenine dinucleotide phosphate oxidase 1 (NOX1) activity by using a NOX1 peptide inhibitor, suggesting that the continuous oxidative stress due to overactivation of NOX1 has a causative role in the underlying pathophysiology. PMID:25437426

  2. Structural features of endocrine active chemicals--A comparison of in vivo and in vitro data.

    PubMed

    Lewin, Geertje; Escher, Sylvia E; van der Burg, Bart; Simetska, Nelly; Mangelsdorf, Inge

    2015-08-01

    Studies on reproductive toxicity need high numbers of test animals. Therefore, we investigated whether chemical structural features (SF) in combination with in vitro data on specific adverse outcome pathways (AOPs) may be used for predicting reproductive toxicity of untested chemicals. Using the OECD Toolbox and expert judgment, we identified 89 structure groups for 275 chemicals for which the results of prenatal developmental toxicity or multigeneration studies were present in the Fraunhofer database on Fertility and Developmental Toxicity in experimental animals (FeDTex) database. Likewise, we evaluated 220 chemicals which had been tested in reporter gene assays on endocrine ((anti)estrogenic and (anti)androgenic) properties in the CALUX(®) test battery. There was a large spread of effect levels for substances within the chemical structure groups for both, in vivo and in vitro results. The groups of highest concern (diphenyl derivatives, planar conjugated systems with fused rings, phenols and organophosphates) correlated quite well, however, between the in vivo and in vitro data on estrogenic activity. For the 56 chemicals represented in both databases, lowest effect doses in vivo correlated well with the estrogenic activity in vitro. These results suggest that a panel of assays covering relevant AOPs and data on metabolism and toxicokinetics may allow prediction of relative reproductive or development toxicity potency within the identified chemical structure groups.

  3. A new irradiation plant in Italy: Technical features and activities performed

    NASA Astrophysics Data System (ADS)

    Tata, A.; Casali, F.; Schwarz, M.

    1998-06-01

    Since 1995, a new Hitesys Co. pilot/industrial irradiation plant has been operating in Aprilia, 50 km south of Rome. The plant, which was jointly designed by ENEA and Hitesys Co., is equipped with a highly flexible automatic materials transport system which is also suitable for continuous process repetition or, when upturned, for double-side material treatment. The main features of the irradiation plant are: - the radiation source: EB-machine LINAC type (s band) with maximum electron beam energy of 10 MeV and beam power of 1000 W; - the bunker: external type, shielded by ordinary concrete, equipped with a suitable entrance/exit maze in order to allow easy handling and managing of materials as well as to facilitate equipment and machinery maintenance and inspection activities. An intense program of R&D activities and technological services has already been performed in relation to industrial processes and environmental applications, as well as many other radiation technology applications, including agriculture related processes.

  4. [Water quality safety of ozonation and biologically activated carbon process in application].

    PubMed

    Qiao, Tie-Jun; Zhang, Xi-Hui

    2009-11-01

    Ozonation and biologically activated carbon process, one of advanced treatment technologies, has been applied in many places at home and abroad. However, some emerging water quality problems appeared in operation. Drinking water treatment plant (6 x 10(5) m3/d) with ozonation and biologically activated carbon process (O3-BAC process) was investigated systematically, including microbial safety, the excessive growth of aquatic microorganism and chemical stability of water quality. And some experiments were done in the pilot plant (10 m3/h) at the same time. O3-BAC process is reliable in microbial safety, but operation management should be enhanced. A good number of aquatic microorganisms grow immoderately during operation of O3-BAC process, which is more serious especially in place with high temperature and humidity. With prolong of runtime, the growth of aquatic microorganisms varies regularly. That is hazardous to water quality safety. When raw water is low with alkalinity, decrease of pH in O3-BAC process is obvious. That will seriously affect on chemical stability. PMID:20063746

  5. Strong Nonadditivity as a Key Structure–Activity Relationship Feature: Distinguishing Structural Changes from Assay Artifacts

    PubMed Central

    2015-01-01

    Nonadditivity in protein–ligand affinity data represents highly instructive structure–activity relationship (SAR) features that indicate structural changes and have the potential to guide rational drug design. At the same time, nonadditivity is a challenge for both basic SAR analysis as well as many ligand-based data analysis techniques such as Free-Wilson Analysis and Matched Molecular Pair analysis, since linear substituent contribution models inherently assume additivity and thus do not work in such cases. While structural causes for nonadditivity have been analyzed anecdotally, no systematic approaches to interpret and use nonadditivity prospectively have been developed yet. In this contribution, we lay the statistical framework for systematic analysis of nonadditivity in a SAR series. First, we develop a general metric to quantify nonadditivity. Then, we demonstrate the non-negligible impact of experimental uncertainty that creates apparent nonadditivity, and we introduce techniques to handle experimental uncertainty. Finally, we analyze public SAR data sets for strong nonadditivity and use recourse to the original publications and available X-ray structures to find structural explanations for the nonadditivity observed. We find that all cases of strong nonadditivity (ΔΔpKi and ΔΔpIC50 > 2.0 log units) with sufficient structural information to generate reasonable hypothesis involve changes in binding mode. With the appropriate statistical basis, nonadditivity analysis offers a variety of new attempts for various areas in computer-aided drug design, including the validation of scoring functions and free energy perturbation approaches, binding pocket classification, and novel features in SAR analysis tools. PMID:25760829

  6. 20 CFR 667.274 - What health and safety standards apply to the working conditions of participants in activities...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false What health and safety standards apply to the...' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR ADMINISTRATIVE PROVISIONS UNDER TITLE... and safety standards apply to the working conditions of participants in activities under title I...

  7. 20 CFR 667.274 - What health and safety standards apply to the working conditions of participants in activities...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false What health and safety standards apply to the...' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR ADMINISTRATIVE PROVISIONS UNDER TITLE... and safety standards apply to the working conditions of participants in activities under title I...

  8. The luminous polycyclic aromatic hydrocarbon emission features: Applications to high redshift galaxies and active galactic nuclei

    NASA Astrophysics Data System (ADS)

    Shipley, Heath Vernon

    (corrected for attenuation using the mono-chromatic rest-frame 24um emission), with a tight scatter of 0.15 dex. The scatter is comparable to that between SFRs derived from the Paalpha and dust-corrected Halpha emission lines, implying the PAH features may be as accurate a SFR indicator as the Hydrogen recombination lines. Because the PAH features are so bright, our PAH SFR calibration enables an efficient way to measure SFRs in distant galaxies with JWST to SFRs as low as ~10 M; yr-1 to z >~ 2. We use Spitzer/IRS observations of PAH features in lensed star-forming galaxies at 1 < z < 3 to demonstrate the utility of the PAHs to derive SFRs as accurate as those available from Paalpha. Chapter 4 is the application of the PAH SFRs for galaxies with AGN to demonstrate the reliability for studies of the co-evolution of star-formation and SMBH accretion. We present a study of the contribution from star-formation in galaxies of varying AGN activity (from pure star-forming galaxies to quasars) as a function of total IR luminosity using a sample of 220 galaxies. We use mid-IR spectroscopy from the Spitzer/IRS and photometry from the MIPS mum, 70mum and 160mum bands with partial coverage of the sample with the Herschel 160mum band for the quasars. The contribution from star-formation to the total IR luminosity implied by the PAH emission decreases with increasing IR luminosity. We find a similar result to previous studies for the correlation between SFR, i.e. PAH luminosity, and AGN luminosity for quasars of LSF [special characters omitted] for the 11.3mum PAH feature only (which has been shown to be the most reliable PAH feature in the vicinity of AGN). This may indicate the PAH luminosity remains a reliable tracer of the SFR for galaxies with strong AGN contributions (i.e. quasars), as we did not subtract off the AGN component before measuring the SFR from the PAH luminosity.

  9. Loss of EBP50 stimulates EGFR activity to induce EMT phenotypic features in biliary cancer cells.

    PubMed

    Clapéron, A; Guedj, N; Mergey, M; Vignjevic, D; Desbois-Mouthon, C; Boissan, M; Saubaméa, B; Paradis, V; Housset, C; Fouassier, L

    2012-03-15

    Scaffold proteins form multiprotein complexes that are central to the regulation of intracellular signaling. The scaffold protein ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) is highly expressed at the plasma membrane of normal biliary epithelial cells and binds epidermal growth factor receptor (EGFR), a tyrosine kinase receptor with oncogenic properties. This study investigated EBP50-EGFR interplay in biliary cancer. We report that in a collection of 106 cholangiocarcinomas, EBP50 was delocalized to the cytoplasm of tumor cells in 66% of the cases. Ectopic expression of EBP50 was correlated with the presence of satellite nodules and with the expression of EGFR, which was at the plasma membrane, implying a loss of interaction with EBP50 in these cases. In vitro, loss of interaction between EBP50 and EGFR was mimicked by EBP50 depletion using a small interfering RNA approach in human biliary carcinoma cells co-expressing the two proteins at their plasma membrane, and in which interaction between EBP50 and EGFR was validated. EBP50 depletion caused an increase in EGFR expression at their surface, and a sustained activation of the receptor and of its downstream effectors (extracellular signal-regulated kinase 1/2, signal transducer and activator of transcription 3) in both basal and EGF-stimulated conditions. Cells lacking EBP50 showed epithelial-to-mesenchymal transition-associated features, including reduction in E-cadherin and cytokeratin-19 expression, induction of S100A4 and of the E-cadherin transcriptional repressor, Slug, and loss of cell polarity. Accordingly, depletion of EBP50 induced the disruption of adherens junctional complexes, the development of lamellipodia structures and the subsequent acquisition of motility properties. All these phenotypic changes were prevented upon inhibition of EGFR tyrosine kinase by gefitinib. These findings indicate that loss of EBP50 at the plasma membrane in tumor cells may contribute to biliary carcinogenesis

  10. Methodological proposal for occupational health and safety actions in research laboratories with nanotechnologies activities.

    PubMed

    Andrade, Luís Renato Balbão; Amaral, Fernando Gonçalves

    2012-01-01

    Nanotechnologies is a multidisciplinary set of techniques to manipulate matter on nanoscale level, more precisely particles below 100 nm whose characteristic due to small size is essentially different from those found in macro form materials. Regarding to these new properties of the materials there are knowledge gaps about the effects of these particles on human organism and the environment. Although it still being considered emerging technology it is growing increasingly fast as well as the number of products using nanotechnologies in some production level and so the number of researchers involved with the subject. Given this scenario and based on literature related, a comprehensive methodology for health and safety at work for researching laboratories with activities in nanotechnologies was developed, based on ILO structure guidelines for safety and health at work system on which a number of nanospecific recommendations were added to. The work intends to offer food for thought on controlling risks associated to nanotechnologies. PMID:22317200

  11. Methodological proposal for occupational health and safety actions in research laboratories with nanotechnologies activities.

    PubMed

    Andrade, Luís Renato Balbão; Amaral, Fernando Gonçalves

    2012-01-01

    Nanotechnologies is a multidisciplinary set of techniques to manipulate matter on nanoscale level, more precisely particles below 100 nm whose characteristic due to small size is essentially different from those found in macro form materials. Regarding to these new properties of the materials there are knowledge gaps about the effects of these particles on human organism and the environment. Although it still being considered emerging technology it is growing increasingly fast as well as the number of products using nanotechnologies in some production level and so the number of researchers involved with the subject. Given this scenario and based on literature related, a comprehensive methodology for health and safety at work for researching laboratories with activities in nanotechnologies was developed, based on ILO structure guidelines for safety and health at work system on which a number of nanospecific recommendations were added to. The work intends to offer food for thought on controlling risks associated to nanotechnologies.

  12. Field studies of safety security rescue technologies through training and response activities

    NASA Astrophysics Data System (ADS)

    Murphy, Robin R.; Stover, Sam

    2006-05-01

    This paper describes the field-oriented philosophy of the Institute for Safety Security Rescue Technology (iSSRT) and summarizes the activities and lessons learned during calendar year 2005 of its two centers: the Center for Robot-Assisted Search and Rescue and the NSF Safety Security Rescue industry/university cooperative research center. In 2005, iSSRT participated in four responses (La Conchita, CA, Mudslides, Hurricane Dennis, Hurricane Katrina, Hurricane Wilma) and conducted three field experiments (NJTF-1, Camp Hurricane, Richmond, MO). The lessons learned covered mobility, operator control units, wireless communications, and general reliability. The work has collectively identified six emerging issues for future work. Based on these studies, a 10-hour, 1 continuing education unit credit course on rescue robotics has been created and is available. Rescue robots and sensors are available for loan upon request.

  13. Development and Validation of Career Development Guidelines by Task/Activity Analysis of Occupational Safety and Health Professions: Industrial Hygiene and Safety Professional. Final Report. Technical Report XII.

    ERIC Educational Resources Information Center

    Vernon, Ralph J.; And Others

    This report summarizes research findings which resulted in development of curricula for occupational safety and health professions based on task/activity analyses and related performance objectives. The first seven chapters focus on the seven objectives. Chapter 1, Literature Review and Selection of Employers, concerns tasks required for…

  14. Activation and maintenance of peripheral semantic features of unambiguous words after right hemisphere brain damage in adults

    PubMed Central

    Tompkins, Connie A.; Fassbinder, Wiltrud; Scharp, Victoria L.; Meigh, Kimberly M.

    2009-01-01

    Background The right cerebral hemisphere (RH) sustains activation of subordinate, secondary, less common, and/or distantly related meanings of words. Much of the pertinent data come from studies of homonyms, but some evidence also suggests that the RH has a unique maintenance function in relation to unambiguous nouns. In a divided visual field priming study, Atchley, Burgess, and Keeney (1999) reported that only left visual field/RH presentation yielded evidence of continuing activation of peripheral semantic features that were incompatible with the most common image or representation of their corresponding nouns (e.g., rotten for “apple”). Activation for weakly related features that were compatible with the dominant representation (e.g., crunchy) was sustained over time regardless of the visual field/hemisphere of initial stimulus input. Several studies report that unilateral right hemisphere brain damage (RHD) in adults affects the RH’s meaning maintenance function, but this work also has centred on homonyms, and/or more recently metonymic and metaphoric polysemous words. Aims The current investigation examined whether RHD deficits in processing secondary and/or distantly related meanings of words, typically observed in studies of homonyms, would extend to peripheral, weakly related semantic features of unambiguous nouns. Methods & Procedures Participants were 28 adults with unilateral RHD from cerebrovascular accident, and 38 adults without brain damage. Participants listened to spoken sentences that ended with an unambiguous noun. Each sentence was followed by a spoken target phoneme string. Targets included peripheral semantic features of the sentence-final noun that were either compatible or incompatible with the dominant mental images of the noun, and were presented at two intervals after that noun. A lexical decision task was used to gauge both the early activation and maintenance of activation for these weakly related semantic features. Outcomes

  15. The novel AKT inhibitor afuresertib shows favorable safety, pharmacokinetics, and clinical activity in multiple myeloma

    PubMed Central

    Spencer, Andrew; Yoon, Sung-Soo; Harrison, Simon J.; Morris, Shannon R.; Smith, Deborah A.; Brigandi, Richard A.; Gauvin, Jennifer; Kumar, Rakesh; Opalinska, Joanna B.

    2014-01-01

    The PI3K/AKT pathway is constitutively active in hematologic malignancies, providing proliferative and antiapoptotic signals and possibly contributing to drug resistance. We conducted an open-label phase 1 study to evaluate the maximum tolerated dose (MTD), safety, pharmacokinetics, and clinical activity of afuresertib—an oral AKT inhibitor—in patients with advanced hematologic malignancies. Seventy-three patients were treated at doses ranging from 25 to 150 mg per day. The MTD was established at 125 mg per day because of 2 dose-limiting toxicities in the 150-mg cohort (liver function test abnormalities). The most frequent adverse events were nausea (35.6%), diarrhea (32.9%), and dyspepsia (24.7%). Maximum plasma concentrations and area under the plasma concentration-time curves from time 0 to 24 hours were generally dose proportional at >75-mg doses; the median time to peak plasma concentrations was 1.5 to 2.5 hours post dose, with a half-life of approximately 1.7 days. Three multiple myeloma patients attained partial responses; an additional 3 attained minimal responses. Clinical activity was also observed in non-Hodgkin lymphoma, Langerhan's cell histiocytosis, and Hodgkin disease. Single-agent afuresertib showed a favorable safety profile and demonstrated clinical activity against hematologic malignancies, including multiple myeloma. This trial was registered at www.clinicaltrials.gov as #NCT00881946. PMID:25075128

  16. Common features and peculiarities of the seismic activity at Phlegraean Fields, Long Valley, and Vesuvius

    USGS Publications Warehouse

    Marzocchi, W.; Vilardo, G.; Hill, D.P.; Ricciardi, G.P.; Ricco, C.

    2001-01-01

    We analyzed and compared the seismic activity that has occurred in the last two to three decades in three distinct volcanic areas: Phlegraean Fields, Italy; Vesuvius, Italy; and Long Valley, California. Our main goal is to identify and discuss common features and peculiarities in the temporal evolution of earthquake sequences that may reflect similarities and differences in the generating processes between these volcanic systems. In particular, we tried to characterize the time series of the number of events and of the seismic energy release in terms of stochastic, deterministic, and chaotic components. The time sequences from each area consist of thousands of earthquakes that allow a detailed quantitative analysis and comparison. The results obtained showed no evidence for either deterministic or chaotic components in the earthquake sequences in Long Valley caldera, which appears to be dominated by stochastic behavior. In contrast, earthquake sequences at Phlegrean Fields and Mount Vesuvius show a deterministic signal mainly consisting of a 24-hour periodicity. Our analysis suggests that the modulation in seismicity is in some way related to thermal diurnal processes, rather than luni-solar tidal effects. Independently from the process that generates these periodicities on the seismicity., it is suggested that the lack (or presence) of diurnal cycles is seismic swarms of volcanic areas could be closely linked to the presence (or lack) of magma motion.

  17. Making Sense of Safety: Cooperative Learning Activities Allow Students Time to Reflect on the Implications of Lab Safety Rules

    ERIC Educational Resources Information Center

    Barrier, Regina

    2005-01-01

    An important part of the science classroom involves teaching students how to safely use tools, techniques, and procedures. As emphasized in the National Science Education Standards, "safety is a fundamental concern in all experiential science" and teachers must "teach students how to engage safely in investigations inside and outside the…

  18. Status of Safety and Environmental Activities in the US Fusion Program

    SciTech Connect

    David A. Petti; Susana Reyes; Lee C. Cadwallader; Jeffery F. Latkowski

    2004-09-01

    This paper presents an overview of recent safety efforts in both magnetic and inertial fusion energy. Safety has been a part of fusion design and operations since the inception of fusion research. Safety research and safety design support have been provided for a variety of experiments in both the magnetic and inertial fusion programs. The main safety issues are reviewed, some recent safety highlights are discussed and the programmatic impacts that safety research has had are presented. Future directions in the safety and environmental area are proposed.

  19. Status of Safety and Environmental Activities in the US Fusion Program

    SciTech Connect

    Petti, D A; Reyes, S; Cadwallader, L C; Latkowski, J F

    2004-09-02

    This paper presents an overview of recent safety efforts in both magnetic and inertial fusion energy. Safety has been a part of fusion design and operations since the inception of fusion research. Safety research and safety design support have been provided for a variety of experiments in both the magnetic and inertial fusion programs. The main safety issues are reviewed, some recent safety highlights are discussed and the programmatic impacts that safety research has had are presented. Future directions in the safety and environmental area are proposed.

  20. Utility of passive photography to objectively audit built environment features of active transport journeys: an observational study

    PubMed Central

    2013-01-01

    Background Active transport can contribute to physical activity accumulation and improved health in adults. The built environment is an established associate of active transport behaviours; however, assessment of environmental features encountered during journeys remains challenging. The purpose of this study was to examine the utility of wearable cameras to objectively audit and quantify environmental features along work-related walking and cycling routes. Methods A convenience sample of employed adults was recruited in New Zealand, in June 2011. Participants wore a SenseCam for all journeys over three weekdays and completed travel diaries and demographic questionnaires. SenseCam images for work-related active transport journeys were coded for presence of environmental features hypothesised to be related to active transport. Differences in presence of features by transport mode and in participant-reported and SenseCam-derived journey duration were determined using two-sample tests of proportion and an independent samples t-test, respectively. Results Fifteen adults participated in the study, yielding 1749 SenseCam images from 30 work-related active transport journeys for coding. Significant differences in presence of features were found between walking and cycling journeys. Almost a quarter of images were uncodeable due to being too dark to determine features. There was a non-significant tendency for respondents to under-report their journey duration. Conclusion This study provides proof of concept for the use of the SenseCam to capture built environment data in real time that may be related to active transportation. Further work is required to test and refine coding methodologies across a range of settings, travel behaviours, and demographic groups. PMID:23575288

  1. Alcohol and Alcohol Safety. Volume II of II. A Curriculum Manual for Elementary Level. A Teacher's Activities Guide.

    ERIC Educational Resources Information Center

    Finn, Peter; Platt, Judith

    This curriculum manual for the elementary school level is the first in a series on alcohol and alcohol safety and is designed as a teacher's activities guide. Each activity provided is a self-contained learning experience which requires varying numbers of class period and focuses on one or more objectives. Activities are numbered consecutively and…

  2. Safety-Related Improvisation in Led Outdoor Activities: An Exploratory Investigation into Its Occurrence and Influencing Factors

    ERIC Educational Resources Information Center

    Trotter, Margaret J.; Salmon, Paul M.; Lenné, Michael G.

    2014-01-01

    The dynamic nature of led outdoor activities means that, despite activity providers' best efforts, activity leaders can be exposed to unanticipated situations for which no procedures exist. Improvisation, the spontaneous, real-time conception and execution of a novel response, has been identified as a potential means of maintaining safety in…

  3. Social safety, self-rated general health and physical activity: changes in area crime, area safety feelings and the role of social cohesion.

    PubMed

    Ruijsbroek, Annemarie; Droomers, Mariël; Groenewegen, Peter P; Hardyns, Wim; Stronks, Karien

    2015-01-01

    The aim of this study was to examine whether changes over time in reported area crime and perceived area safety were related to self-rated general health and physical activity (PA), in order to provide support for a causal relationship between social safety and health. Additionally, we investigated whether social cohesion protects the residents against the negative impact of unsafe areas on health and PA. Multilevel logistic regression analyses were performed on Dutch survey data, including 47,926 respondents living in 2974 areas. An increase in area level unsafety feelings between 2009 and 2011 was associated with more people reporting poor general health in 2012 in that area, but was not related to PA. Changes in reported area crime were not related to either poor general health or PA. The social cohesion in the area did not modify the effect of changes in social safety on health and PA. The results suggest that tackling feelings of unsafety in an area might contribute to the better general health of the residents. Because changes in area social safety were not associated with PA, we found no leads that such health benefits were achieved through an increase in physical activity.

  4. Active and Passive Fatigue in Simulated Driving: Discriminating Styles of Workload Regulation and Their Safety Impacts

    PubMed Central

    Saxby, Dyani J.; Matthews, Gerald; Warm, Joel S.; Hitchcock, Edward M.; Neubauer, Catherine

    2015-01-01

    Despite the known dangers of driver fatigue, it is a difficult construct to study empirically. Different forms of task-induced fatigue may differ in their effects on driver performance and safety. Desmond and Hancock (2001) defined active and passive fatigue states that reflect different styles of workload regulation. In 2 driving simulator studies we investigated the multidimensional subjective states and safety outcomes associated with active and passive fatigue. Wind gusts were used to induce active fatigue, and full vehicle automation to induce passive fatigue. Drive duration was independently manipulated to track the development of fatigue states over time. Participants were undergraduate students. Study 1 (N = 108) focused on subjective response and associated cognitive stress processes, while Study 2 (N = 168) tested fatigue effects on vehicle control and alertness. In both studies the 2 fatigue manipulations produced different patterns of subjective response reflecting different styles of workload regulation, appraisal, and coping. Active fatigue was associated with distress, overload, and heightened coping efforts, whereas passive fatigue corresponded to large-magnitude declines in task engagement, cognitive underload, and reduced challenge appraisal. Study 2 showed that only passive fatigue reduced alertness, operationalized as speed of braking and steering responses to an emergency event. Passive fatigue also increased crash probability, but did not affect a measure of vehicle control. Findings support theories that see fatigue as an outcome of strategies for managing workload. The distinction between active and passive fatigue is important for assessment of fatigue and for evaluating automated driving systems which may induce dangerous levels of passive fatigue. PMID:24041288

  5. Autobiographical Planning and the Brain: Activation and Its Modulation by Qualitative Features.

    PubMed

    Spreng, R Nathan; Gerlach, Kathy D; Turner, Gary R; Schacter, Daniel L

    2015-11-01

    To engage in purposeful behavior, it is important to make plans, which organize subsequent actions. Most studies of planning involve "look-ahead" puzzle tasks that are unrelated to personal goals. We developed a task to assess autobiographical planning, which involves the formulation of personal plans in response to real-world goals, and examined autobiographical planning in 63 adults during fMRI scanning. Autobiographical planning was found to engage the default network, including medial-temporal lobe and midline structures, and executive control regions in lateral pFC and parietal cortex and caudate. To examine how specific qualitative features of autobiographical plans modulate neural activity, we performed parametric modulation analyses. Ratings of plan detail, novelty, temporal distance, ease of plan formulation, difficulty in goal completion, and confidence in goal accomplishment were used as covariates in six hierarchical linear regression models. This modeling procedure removed shared variance among the ratings, allowing us to determine the independent relationship between ratings of interest and trial-wise BOLD signal. We found that specific autobiographical planning, describing a detailed, achievable, and actionable planning process for attaining a clearly envisioned future, recruited both default and frontoparietal brain regions. In contrast, abstract autobiographical planning, plans that were constructed from more generalized semantic or affective representations of a less tangible and distant future, involved interactions among default, sensory perceptual, and limbic brain structures. Specific qualities of autobiographical plans are important predictors of default and frontoparietal control network engagement during plan formation and reflect the contribution of mnemonic and executive control processes to autobiographical planning.

  6. Autobiographical Planning and the Brain: Activation and Its Modulation by Qualitative Features.

    PubMed

    Spreng, R Nathan; Gerlach, Kathy D; Turner, Gary R; Schacter, Daniel L

    2015-11-01

    To engage in purposeful behavior, it is important to make plans, which organize subsequent actions. Most studies of planning involve "look-ahead" puzzle tasks that are unrelated to personal goals. We developed a task to assess autobiographical planning, which involves the formulation of personal plans in response to real-world goals, and examined autobiographical planning in 63 adults during fMRI scanning. Autobiographical planning was found to engage the default network, including medial-temporal lobe and midline structures, and executive control regions in lateral pFC and parietal cortex and caudate. To examine how specific qualitative features of autobiographical plans modulate neural activity, we performed parametric modulation analyses. Ratings of plan detail, novelty, temporal distance, ease of plan formulation, difficulty in goal completion, and confidence in goal accomplishment were used as covariates in six hierarchical linear regression models. This modeling procedure removed shared variance among the ratings, allowing us to determine the independent relationship between ratings of interest and trial-wise BOLD signal. We found that specific autobiographical planning, describing a detailed, achievable, and actionable planning process for attaining a clearly envisioned future, recruited both default and frontoparietal brain regions. In contrast, abstract autobiographical planning, plans that were constructed from more generalized semantic or affective representations of a less tangible and distant future, involved interactions among default, sensory perceptual, and limbic brain structures. Specific qualities of autobiographical plans are important predictors of default and frontoparietal control network engagement during plan formation and reflect the contribution of mnemonic and executive control processes to autobiographical planning. PMID:26102226

  7. Assessing low-activity faults for the seismic safety of dams

    SciTech Connect

    Page, W.D.; Savage, W.U.; McLaren, M.K.

    1995-12-31

    Dams have been a familiar construct in the northern Sierra Nevada range in California (north of the San Joaquin River) since the forty-niners and farmers diverted water to their gold mines and farms in the mid 19th century. Today, more than 370 dams dot the region from the Central Valley to the eastern escarpment. Fifty-five more dam streams on the eastern slope. The dams are of all types: 240 earth fill; 56 concrete gravity; 45 rock and earth fills; 35 rock fill; 14 concrete arch; 9 hydraulic fill; and 29 various other types. We use the northern Sierra Nevada to illustrate the assessment of low-activity faults for the seismic safety of dams. The approach, techniques, and methods of evaluation are applicable to other regions characterized by low seismicity and low-activity faults having long recurrence intervals. Even though several moderate earthquakes had shaken the Sierra Nevada since 1849 (for example, the 1875 magnitude 5.8 Honey Lake and the 1909 magnitudes 5 and 5.5 Downieville earthquakes), seismic analyses for dams in the area generally were not performed prior to the middle of this century. Following the 1971 magnitude 6.7 San Fernando earthquake, when the hydraulic-fill Lower Van Norman Dam in southern California narrowly escaped catastrophic failure, the California Division of Safety of Dams and the Federal Energy Regulatory Commission required seismic safety to be addressed with increasing rigor. In 1975, the magnitude 5.7 Oroville earthquake on the Cleveland Hill fault near Oroville Dam in the Sierra Nevada foothills, showed convincingly that earthquakes and surface faulting could occur within the range. Following this event, faults along the ancient Foothills fault system have been extensively investigated at dam sites.

  8. A skin sensitization safety assessment of a new bleach activator technology in detergent applications.

    PubMed

    Roggeband, R; Helmlinger, G; Smith, I; Wilhelm, K-P; Ryan, C A; Gerberick, G F

    2002-04-01

    A new chemical called nonanoyl amido caproylacid oxybenzenesulphonate (NACAOBS) is being developed for use as a bleach activator in laundry detergents. Bleach activators, like NACAOBS, are typically used at levels between 2% and 6% in laundry detergents. NACAOBS is stable in aqueous solutions, but undergoes rapid perhydrolysis when combined with water and peroxygen bleach in laundry detergents. Animal testing demonstrated that NACAOBS, as a raw material, is a weak skin sensitizer. Clinical testing, including extended simulated laundry pretreatment, human repeat insult patch testing and home use testing was then undertaken, following sufficient reassurance of 1) the weak sensitization potential of the substance, 2) its rapid degradation in laundry wash solutions and, consequently, 3) low-to-negligible consumer dermal exposures to the native substance. Results confirmed the skin sensitization safety profile of laundry detergents containing NACAOBS, namely the absence of any reaction suggestive of contact sensitization (even under exaggerated dermal exposure conditions in a detergent matrix), and a skin compatibility profile comparable to that of current detergents. Further confirmation of the skin safety profile was obtained from a successful 12-month market test of a granular detergent containing 3.6% of the new substance, during which not a single adverse skin reaction was reported. In addition, NOBS (an oxybenzenesulphonate structural analogue to NACAOBS) has similar toxicological properties and has been safely marketed in detergents at similar levels for many years. It can be concluded that the likelihood of NACAOBS to induce skin sensitization or even elicit allergic reactions in consumer detergent use scenarios is negligible.

  9. Study of EGNOS safety of life service during the period of solar maximum activity

    NASA Astrophysics Data System (ADS)

    Grzegorzewski, Marek; Swiatek, Anna; Oszczak, Stanislaw; Ciecko, Adam; Cwiklak, Janusz

    2012-12-01

    The Satellite Base Augmentation System (SBAS) - EGNOS (European Geostationary Navigation Overlay Service) has been certified for Safety of Life (SoL) service for aircraft navigation since 2nd of March 2011. Unfortunately for the territory of Poland, located at the edge of EGNOS service area, the quality of the service corrections are still not sufficient for aircraft navigation requirements. Years 2012 and 2013 are forecasted as a maximum of solar activity in a 11-year solar cycle. This time period will be the chance to perform the first tests for the EGNOS Safety of Life service quality in disturbed ionospheric conditions. During the previous maximum of solar activity, the storm on 30 October 2003 resulted in the inability to use WAAS corrections for more than 12 hours. This was caused by a very large gradient of disturbances and its' very sharp boundaries - vertical TEC (VTEC) varied from ~ 40 to ~ 120 TECU (TEC units) within an hour (over ~ 150 km distance). These circumstances gave the opportunity to carry out the test flights to examine the navigation parameters obtained for EGNOS SoL service in disturbed ionospheric conditions. The paper presents project proposal of study and analyses of such fundamental navigation parameters as: accuracy of determined position, availability, continuity and integrity, determined for selected disturbances in relation to quiet conditions. It can give a possibility to estimate of the quality of EGNOS SoL service in Polish airspace during the different phases of flight and its resistance to critical ionospheric conditions.

  10. Monoaminergic tone supports conductance correlations and stabilizes activity features in pattern generating neurons of the lobster, Panulirus interruptus.

    PubMed

    Krenz, Wulf-Dieter; Parker, Anna R; Rodgers, Edmund; Baro, Deborah J

    2015-01-01

    Experimental and computational studies demonstrate that different sets of intrinsic and synaptic conductances can give rise to equivalent activity patterns. This is because the balance of conductances, not their absolute values, defines a given activity feature. Activity-dependent feedback mechanisms maintain neuronal conductance correlations and their corresponding activity features. This study demonstrates that tonic nM concentrations of monoamines enable slow, activity-dependent processes that can maintain a correlation between the transient potassium current (I(A) and the hyperpolarization activated current (Ih) over the long-term (i.e., regulatory change persists for hours after removal of modulator). Tonic 5 nM DA acted through an RNA interference silencing complex (RISC)- and RNA polymerase II-dependent mechanism to maintain a long-term positive correlation between I(A) and Ih in the lateral pyloric neuron (LP) but not in the pyloric dilator neuron (PD). In contrast, tonic 5 nM 5HT maintained a RISC-dependent positive correlation between I(A) and Ih in PD but not LP over the long-term. Tonic 5 nM OCT maintained a long-term negative correlation between I(A) and Ih in PD but not LP; however, it was only revealed when RISC was inhibited. This study also demonstrated that monoaminergic tone can also preserve activity features over the long-term: the timing of LP activity, LP duty cycle and LP spike number per burst were maintained by tonic 5 nM DA. The data suggest that low-level monoaminergic tone acts through multiple slow processes to permit cell-specific, activity-dependent regulation of ionic conductances to maintain conductance correlations and their corresponding activity features over the long-term. PMID:26539083

  11. Monoaminergic tone supports conductance correlations and stabilizes activity features in pattern generating neurons of the lobster, Panulirus interruptus

    PubMed Central

    Krenz, Wulf-Dieter; Parker, Anna R.; Rodgers, Edmund; Baro, Deborah J.

    2015-01-01

    Experimental and computational studies demonstrate that different sets of intrinsic and synaptic conductances can give rise to equivalent activity patterns. This is because the balance of conductances, not their absolute values, defines a given activity feature. Activity-dependent feedback mechanisms maintain neuronal conductance correlations and their corresponding activity features. This study demonstrates that tonic nM concentrations of monoamines enable slow, activity-dependent processes that can maintain a correlation between the transient potassium current (IA) and the hyperpolarization activated current (Ih) over the long-term (i.e., regulatory change persists for hours after removal of modulator). Tonic 5 nM DA acted through an RNA interference silencing complex (RISC)- and RNA polymerase II-dependent mechanism to maintain a long-term positive correlation between IA and Ih in the lateral pyloric neuron (LP) but not in the pyloric dilator neuron (PD). In contrast, tonic 5 nM 5HT maintained a RISC-dependent positive correlation between IA and Ih in PD but not LP over the long-term. Tonic 5 nM OCT maintained a long-term negative correlation between IA and Ih in PD but not LP; however, it was only revealed when RISC was inhibited. This study also demonstrated that monoaminergic tone can also preserve activity features over the long-term: the timing of LP activity, LP duty cycle and LP spike number per burst were maintained by tonic 5 nM DA. The data suggest that low-level monoaminergic tone acts through multiple slow processes to permit cell-specific, activity-dependent regulation of ionic conductances to maintain conductance correlations and their corresponding activity features over the long-term. PMID:26539083

  12. Evaluating life-safety risk of fieldwork at New Zealand's active volcanoes

    NASA Astrophysics Data System (ADS)

    Deligne, Natalia; Jolly, Gill; Taig, Tony; Webb, Terry

    2014-05-01

    Volcano observatories monitor active or potentially active volcanoes. Although the number and scope of remote monitoring instruments and methods continues to grow, in-person field data collection is still required for comprehensive monitoring. Fieldwork anywhere, and especially in mountainous areas, contains an element of risk. However, on volcanoes with signs of unrest, there is an additional risk of volcanic activity escalating while on site, with potentially lethal consequences. As an employer, a volcano observatory is morally and sometimes legally obligated to take reasonable measures to ensure staff safety and to minimise occupational risk. Here we present how GNS Science evaluates life-safety risk for volcanologists engaged in fieldwork on New Zealand volcanoes with signs of volcanic unrest. Our method includes several key elements: (1) an expert elicitation for how likely an eruption is within a given time frame, (2) quantification of, based on historical data when possible, given a small, moderate, or large eruption, the likelihood of exposure to near-vent processes, ballistics, or surge at various distances from the vent, and (3) estimate of fatality rate given exposure to these volcanic hazards. The final product quantifies hourly fatality risk at various distances from a volcanic vent; various thresholds of risk (for example, zones with more than 10-5 hourly fatality risk) trigger different levels of required approval to undertake work. Although an element of risk will always be present when conducting fieldwork on potentially active volcanoes, this is a first step towards providing objective guidance for go/no go decisions for volcanic monitoring.

  13. Ethnic Minority Children’s Active Commuting to School and Association with Physical Activity and Pedestrian Safety Behaviors*

    PubMed Central

    Mendoza, Jason A.; Watson, Kathy; Baranowski, Tom; Nicklas, Theresa A.; Uscanga, Doris K.; Nguyen, Nga; Hanfling, Marcus J.

    2011-01-01

    -to-vigorous physical activity, active commuting to school was positively associated (std. beta = 0.31, p <0.001). Among the Latino subsample, child acculturation was negatively associated with active commuting to school (std. beta = −0.23, p=0.01). With regard to school-level pedestrian safety observations, 37% of students stopped at the curb and 2.6% looked left-right-left before crossing the street. Conclusion Although still below national goals, the rate of active commuting was relatively high, while the rate of some pedestrian safety behaviors was low among this low-income, ethnic minority population. Programs and policies to encourage safe active commuting to school are warranted and should consider the influence of parents, acculturation, and ethnicity. PMID:21874160

  14. Measuring hospital-wide activity volume for patient safety and infection control: a multi-centre study in Japan

    PubMed Central

    Hayashida, Kenshi; Imanaka, Yuichi; Fukuda, Haruhisa

    2007-01-01

    Background In Japan, as in many other countries, several quality and safety assurance measures have been implemented since the 1990's. This has occurred in spite of cost containment efforts. Although government and hospital decision-makers demand comprehensive analysis of these activities at the hospital-wide level, there have been few studies that actually quantify them. Therefore, the aims of this study were to measure hospital-wide activities for patient safety and infection control through a systematic framework, and to identify the incremental volume of these activities implemented over the last five years. Methods Using the conceptual framework of incremental activity corresponding to incremental cost, we defined the scope of patient safety and infection control activities. We then drafted a questionnaire to analyze these realms. After implementing the questionnaire, we conducted several in-person interviews with managers and other staff in charge of patient safety and infection control in seven acute care teaching hospitals in Japan. Results At most hospitals, nurses and clerical employees acted as the main figures in patient safety practices. The annual amount of activity ranged from 14,557 to 72,996 person-hours (per 100 beds: 6,240; per 100 staff: 3,323) across participant hospitals. Pharmacists performed more incremental activities than their proportional share. With respect to infection control activities, the annual volume ranged from 3,015 to 12,196 person-hours (per 100 beds: 1,141; per 100 staff: 613). For infection control, medical doctors and nurses tended to perform somewhat more of the duties relative to their share. Conclusion We developed a systematic framework to quantify hospital-wide activities for patient safety and infection control. We also assessed the incremental volume of these activities in Japanese hospitals under the reimbursement containment policy. Government and hospital decision makers can benefit from this type of analytic

  15. Direct stroke unit admission of intravenous tissue plasminogen activator: safety, clinical outcome, and hospital cost savings

    PubMed Central

    Alexandrov, Anne W.; Coleman, Kisha C.; Palazzo, Paola; Shahripour, Reza Bavarsad; Alexandrov, Andrei V.

    2016-01-01

    Background: In the USA, stable intravenous tissue plasminogen activator (IV tPA) patients have traditionally been cared for in an intensive care unit (ICU). We examined the safety of using an acuity-adaptable stroke unit (SU) to manage IV tPA patients. Methods: We conducted an observational study of consecutive patients admitted to our acuity-adaptable SU over the first 3 years of operation. Safety was assessed by symptomatic intracerebral hemorrhage (sICH) rates, systemic hemorrhage (SH) rates, tPA-related deaths, and transfers from SU to ICU; cost savings and length of stay (LOS) were determined. Results: We admitted 333 IV tPA patients, of which 302 were admitted directly to the SU. A total of 31 (10%) patients had concurrent systemic hemodynamic or pulmonary compromise warranting direct ICU admission. There were no differences in admission National Institutes of Health Stroke Scale scores between SU and ICU patients (9.0 versus 9.5, respectively). Overall sICH rate was 3.3% (n = 10) and SH rate was 2.9 (n = 9), with no difference between SU and ICU patients. No tPA-related deaths occurred, and no SU patients required transfer to the ICU. Estimated hospital cost savings were US$362,400 for ‘avoided’ ICU days, and hospital LOS decreased significantly (p = 0.001) from 9.8 ± 15.6 days (median 5) in year 1, to 5.2 ± 4.8 days (median 3) by year 3. Conclusions: IV tPA patients may be safely cared for in a SU when nurses undergo extensive education to ensure clinical competence. Use of the ICU solely for monitoring may constitute significant overuse of system resources at an expense that is not associated with additional safety benefit. PMID:27366237

  16. Pharmacokinetics, safety and tolerability of triflusal and its main active metabolite HTB in healthy Chinese subjects.

    PubMed

    Wang, M; Zhang, Q; Huang, M; Zong, S; Hua, W; Zhou, W

    2014-05-01

    Triflusal presents comparable antiplatelet activity to aspirin while presenting a more favourable safety profile, and is used in the treatment of thrombosis. The study aimed to evaluate the pharmacokinetics and safety of triflusal and its major metabolite 2-(hydroxyl)-4-(trifluoromethyl)- benzoic acid (HTB) in healthy Chinese subjects.30 healthy subjects were recruited in this randomized, single-center, and open-label, parallel, single ascending doses (300, 600, 900 mg) and multiple doses (600 mg, once daily for 7 days) study. Plasma samples were analyzed with a validated liquid chromatography tandem mass spectrometry (LC/MS/MS) method. Safety was assessed by adverse events, ECG, laboratory testing, and vital signs.Triflusal was safe and well tolerated. After single-dose administration, triflusal was rapidly absorbed with a mean Tmax of 0.55-0.92 h and a mean t1/2 kel of 0.35-0.65 h, HTB was absorbed with a mean Tmax of 2.35-3.03 h and a mean t1/2 kel of 52.5-65.57 h. Cmax and AUC for triflusal and HTB were approximately dose proportional over the 300-900 mg dose range. In the steady state, the accumulation index (R) indicated that the exposure of triflusal increased slightly with repeated dosing, and the exposure of HTB increased obviously. 3 adverse events certainly related to the investigational drugs occurred in the multiple-dose phase.Following oral dosing under fasting condition, triflusal is promptly absorbed and rapidly depleted from the systemic circulation. HTB is quickly generated from triflusal and slowly eliminated. Triflusal accumulates slightly in the body. HTB plasma concentration builds up progressively toward steady-state. PMID:24105106

  17. Some features of the bioelectric activity of the muscles with prolonged hypokinesia

    NASA Technical Reports Server (NTRS)

    Belaya, N. A.; Amirov, R. Z.; Shaposhnikov, Y. A.; Lebedeva, I. P.; Sologub, B. S.

    1978-01-01

    The effects of prolonged hypokinesia, brought on by confinement to bed and the attendant lack of motor activity, on the bioelectric activity of muscles are studied. Electromyographic measurements of amplitude and frequency indicators of muscular bioelectric activity were analyzed.

  18. Building Model NASA Satellites: Elementary Students Studying Science Using a NASA-Themed Transmedia Book Featuring Digital Fabrication Activities

    ERIC Educational Resources Information Center

    Tillman, Daniel; An, Song; Boren, Rachel; Slykhuis, David

    2014-01-01

    This study assessed the impact of nine lessons incorporating a NASA-themed transmedia book featuring digital fabrication activities on 5th-grade students (n = 29) recognized as advanced in mathematics based on their academic record. Data collected included a pretest and posttest of science content questions taken from released Virginia Standards…

  19. A triaxial accelerometer-based physical-activity recognition via augmented-signal features and a hierarchical recognizer.

    PubMed

    Khan, Adil Mehmood; Lee, Young-Koo; Lee, Sungyoung Y; Kim, Tae-Seong

    2010-09-01

    Physical-activity recognition via wearable sensors can provide valuable information regarding an individual's degree of functional ability and lifestyle. In this paper, we present an accelerometer sensor-based approach for human-activity recognition. Our proposed recognition method uses a hierarchical scheme. At the lower level, the state to which an activity belongs, i.e., static, transition, or dynamic, is recognized by means of statistical signal features and artificial-neural nets (ANNs). The upper level recognition uses the autoregressive (AR) modeling of the acceleration signals, thus, incorporating the derived AR-coefficients along with the signal-magnitude area and tilt angle to form an augmented-feature vector. The resulting feature vector is further processed by the linear-discriminant analysis and ANNs to recognize a particular human activity. Our proposed activity-recognition method recognizes three states and 15 activities with an average accuracy of 97.9% using only a single triaxial accelerometer attached to the subject's chest.

  20. [Analysis of decompression safety during extravehicular activity of astronauts in the light of probability theory].

    PubMed

    Nikolaev, V P; Katuntsev, V P

    1998-01-01

    Objectives of the study were comparative assessment of the risk of decompression sickness (DCS) in human subjects during shirt-sleeve simulation of extravehicular activity (EVA) following Russian and U.S. protocols, and analysis of causes of the difference between real and simulated EVA decompression safety. To this end, DCS risk during exposure to a sing-step decompression was estimated with an original method. According to the method, DCS incidence is determined by distribution of nucleation efficacy index (z) in the worst body tissues and its critical values (zm) as a function of initial nitrogen tension in these tissues and final ambient pressure post decompression. Gaussian distribution of z values was calculated basing on results of the DCS risk evaluation on the U.S. EVA protocol in an unsuited chamber test with various pre-breath procedures (Conkin et al., 1987). Half-time of nitrogen washout from the worst tissues was presumed to be 480 min. Calculated DCS risk during short-sleeve EVA simulation by the Russian and U.S. protocols with identical physical loading made up 19.2% and 23.4%, respectively. Effects of the working spacesuit pressure, spacesuit rigidity, metabolic rates during operations in EVA space suit, transcutaneous nitrogen exchange in the oxygen atmosphere of space suit, microgravity, analgesics, short compression due to spacesuit leak tests on the eye of EVA are discussed. Data of the study illustrate and advocate for high decompression safety of current Russian and U.S. EVA protocols.

  1. DOE standard: Integration of environment, safety, and health into facility disposition activities. Volume 2: Appendices

    SciTech Connect

    1998-05-01

    This volume contains the appendices that provide additional environment, safety, and health (ES and H) information to complement Volume 1 of this Standard. Appendix A provides a set of candidate DOE ES and H directives and external regulations, organized by hazard types that may be used to identify potentially applicable directives to a specific facility disposition activity. Appendix B offers examples and lessons learned that illustrate implementation of ES and H approaches discussed in Section 3 of Volume 1. Appendix C contains ISMS performance expectations to guide a project team in developing and implementing an effective ISMS and in developing specific performance criteria for use in facility disposition. Appendix D provides guidance for identifying potential Applicable or Relevant and Appropriate Requirements (ARARs) when decommissioning facilities fall under the Comprehensive Environmental Response, Compensation, Liability Act (CERCLA) process. Appendix E discusses ES and H considerations for dispositioning facilities by privatization. Appendix F is an overview of the WSS process. Appendix G provides a copy of two DOE Office of Nuclear Safety Policy and Standards memoranda that form the bases for some of the guidance discussed within the Standard. Appendix H gives information on available hazard analysis techniques and references. Appendix I provides a supplemental discussion to Sections 3.3.4, Hazard Baseline Documentation, and 3.3.6, Environmental Permits. Appendix J presents a sample readiness evaluation checklist.

  2. Sporicidal activity of an improved iodide formulation and suggestions regarding the biological safety cabinet.

    PubMed

    Kida, Nori

    2009-06-01

    The sporicidal activity of an improved iodide formulation based on a previously reported agent (Kida et al., 2004, tentatively designated as the KMT reagent) which is composed of 50 mM EDTA-2Na, 50 mM ferric chloride hexahydrate (FeCl3.6H2O), 50 mM potassium iodide (KI) and 50% ethanol in 0.85% NaCl solution at pH 0.3 with hydrochloric acid, was examined in the liquid and vapor phases. The improved iodide formulation subject to distillation (tentatively designated as the distilled KMT reagent: pH around 3) showed comparable sporicidal activity with the KMT reagent. As for the dilution effect, dilution at 1:2 showed more potent sporicidal activity than the undiluted one. It achieved complete disinfection with a treatment for 5 min at 20 degrees C and for 60 min at 5 degrees C. Even at a ratio of 1:100, the dilutions showed significant sporicidal activities at 37 degrees C. The experiment on the disinfection of the biological safety cabinet (Class II type A) as a practical possibility showed that pretreatment with 400 ml of water vapor treatment, and a mixture of 300 ml of this reagent and 150 ml of water in vapor phase achieved complete disinfection after a 24 h-decontamination process. The distilled KMT reagent may be useful for disinfecting against various contaminated materials and sites in both the liquid phase and vapor phase. PMID:19579660

  3. EH&S annual report: Summary of activities Environment, Health and Safety Division, 1992

    SciTech Connect

    Not Available

    1994-03-01

    This report presents an overview of the environment, safety, and health program in operation at the Lawrence Berkeley Laboratory. description of research in environmental science, remediation, waste management, safety, health services, radiation assessment, and emergency plans are provided.

  4. Activity Participation and Sensory Features among Children with Autism Spectrum Disorders

    ERIC Educational Resources Information Center

    Little, Lauren M.; Ausderau, Karla; Sideris, John; Baranek, Grace T.

    2015-01-01

    Sensory features are highly prevalent among children with autism spectrum disorders (ASD) and have been shown to cluster into four patterns of response, including hyperresponsiveness, hyporesponsiveness, enhanced perception, and sensory interests, repetitions and seeking behaviors. Given the lack of large-scale research on the differential effects…

  5. Current environmental, health, safety, and socioeconomic research activities related to oil shale: draft

    SciTech Connect

    Not Available

    1980-09-01

    This document was prepared for DOE Resource Applications. It provides a compilation of information on current environmental, health, safety and socioeconomic research activities related to oil shale. The information is the most recent available through August 29, 1980. Included are the following: (1) project title; (2) adminstering agency; (3) contractor; (4) project status; (5) funding level; (6) project schedule; (7) deliverable; and (8) key personnel. The data contained in these reports can be used in environmental impact analyses relating oil shale to various incentives given in the Alternative Fuels Bill. The information provided was obtained from computer search printouts, review of respective agency documents and communication with agency personnel. A complete list of references is provided. The sponsoring organizations include the Department of Energy, the Environmental Protection Agency, the Department of Agriculture, and the Department of Interior.

  6. Interfacial Reaction-Driven Formation of Silica Carbonate Biomorphs with Subcellular Topographical Features and Their Biological Activity.

    PubMed

    Wang, Guocheng; Zhao, Xiaobing; Möller, Marco; Moya, Sergio E

    2015-10-28

    We report the interfacial reaction-driven formation of micro/nanostructured strontium carbonate (SrCO3) biomorphs with subcellular topographical features on strontium zinc silicate (Sr2ZnSi2O7) biomedical coatings and explore their potential use in bone tissue engineering. The resulting SrCO3 crystals build a well-integrated scaffold surface that not only prevents burst release of ions from the coating but also presents nanotopographical features similar to cellular filopodia. The surface with biomorphic crystals enhances osteoblast adhesion, upregulates the alkaline phosphatase activity, and increases collagen production, highlighting the potential of the silica carbonate biomorphs for tissue regeneration. PMID:26455427

  7. Interfacial Reaction-Driven Formation of Silica Carbonate Biomorphs with Subcellular Topographical Features and Their Biological Activity.

    PubMed

    Wang, Guocheng; Zhao, Xiaobing; Möller, Marco; Moya, Sergio E

    2015-10-28

    We report the interfacial reaction-driven formation of micro/nanostructured strontium carbonate (SrCO3) biomorphs with subcellular topographical features on strontium zinc silicate (Sr2ZnSi2O7) biomedical coatings and explore their potential use in bone tissue engineering. The resulting SrCO3 crystals build a well-integrated scaffold surface that not only prevents burst release of ions from the coating but also presents nanotopographical features similar to cellular filopodia. The surface with biomorphic crystals enhances osteoblast adhesion, upregulates the alkaline phosphatase activity, and increases collagen production, highlighting the potential of the silica carbonate biomorphs for tissue regeneration.

  8. Towards global age-friendly cities: determining urban features that promote active aging.

    PubMed

    Plouffe, Louise; Kalache, Alexandre

    2010-09-01

    At the same time as cities are growing, their share of older residents is increasing. To engage and assist cities to become more "age-friendly," the World Health Organization (WHO) prepared the Global Age-Friendly Cities Guide and a companion "Checklist of Essential Features of Age-Friendly Cities". In collaboration with partners in 35 cities from developed and developing countries, WHO determined the features of age-friendly cities in eight domains of urban life: outdoor spaces and buildings; transportation; housing; social participation; respect and social inclusion; civic participation and employment; communication and information; and community support and health services. In 33 cities, partners conducted 158 focus groups with persons aged 60 years and older from lower- and middle-income areas of a locally defined geographic area (n = 1,485). Additional focus groups were held in most sites with caregivers of older persons (n = 250 caregivers) and with service providers from the public, voluntary, and commercial sectors (n = 515). No systematic differences in focus group themes were noted between cities in developed and developing countries, although the positive, age-friendly features were more numerous in cities in developed countries. Physical accessibility, service proximity, security, affordability, and inclusiveness were important characteristics everywhere. Based on the recurring issues, a set of core features of an age-friendly city was identified. The Global Age-Friendly Cities Guide and companion "Checklist of Essential Features of Age-Friendly Cities" released by WHO serve as reference for other communities to assess their age readiness and plan change.

  9. Efficacy and Safety of Rituximab in Moderately-to-Severely Active Systemic Lupus Erythematosus

    PubMed Central

    Merrill, Joan T.; Neuwelt, C. Michael; Wallace, Daniel J.; Shanahan, Joseph C.; Latinis, Kevin M.; Oates, James C.; Utset, Tammy O.; Gordon, Caroline; Isenberg, David A.; Hsieh, Hsin-Ju; Zhang, David; Brunetta, Paul G.

    2015-01-01

    Objective B cells are likely to contribute to the pathogenesis of systemic lupus erythematosus (SLE), and rituximab induces depletion of B cells. The Exploratory Phase II/III SLE Evaluation of Rituximab (EXPLORER) trial tested the efficacy and safety of rituximab versus placebo in patients with moderately-to-severely active extrarenal SLE. Methods Patients entered with ≥1 British Isles Lupus Assessment Group (BILAG) A score or ≥2 BILAG B scores despite background immunosuppressant therapy, which was continued during the trial. Prednisone was added and subsequently tapered. Patients were randomized at a ratio of 2:1 to receive rituximab (1,000 mg) or placebo on days 1, 15, 168, and 182. Results In the intent-to-treat analysis of 257 patients, background treatment was evenly distributed among azathioprine, mycophenolate mofetil, and methotrexate. Fifty-three percent of the patients had ≥1 BILAG A score at entry, and 57% of the patients were categorized as being steroid dependent. No differences were observed between placebo and rituximab in the primary and secondary efficacy end points, including the BILAG-defined response, in terms of both area under the curve and landmark analyses. A beneficial effect of rituximab on the primary end point was observed in the African American and Hispanic subgroups. Safety and tolerability were similar in patients receiving placebo and those receiving rituximab. Conclusion The EXPLORER trial enrolled patients with moderately-to-severely active SLE and used aggressive background treatment and sensitive cutoffs for nonresponse. No differences were noted between placebo and rituximab in the primary and secondary end points. Further evaluation of patient subsets, biomarkers, and exploratory outcome models may improve the design of future SLE clinical trials. PMID:20039413

  10. Evaluation of Antioxidant, Immunomodulatory Activities, and Safety of Ethanol Extract and Fractions of Gongronema latifolium Fruit

    PubMed Central

    Agwaramgbo, Amanze; Ilodigwe, Emmanuel Emeka; Ajaghaku, Daniel Lotanna; Onuorah, Maureen Ugochukwu; Mbagwu, Sonne Ikechukwu

    2014-01-01

    Gongronema latifolium fruit has wide application in ethnomedicine, especially in maintaining healthy living and general body healing. We therefore investigated the antioxidant, immunomodulatory activities, and safety of its ethanol extract and fractions. The in vitro antioxidant activities of the extract and fractions were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) test while in vivo activities were determined using carbon tetrachloride (CCL4) induced oxidative stress. Cell and humoral mediated immune responses were also evaluated together with toxicity studies. The extract, ethyl acetate, and methanol fractions showed inhibition of DPPH radical with IC50s 120, 90, and 60 μg/mL, respectively. Methanol fraction at 200 mg/kg produced significant (P < 0.05) inhibition of lipid peroxidation (MDA conc. 1.2 μmol/L) compared to control (2.8 μmol/L). Both ethyl acetate and methanol fractions at 200 mg/kg produced significant (P < 0.05) phagocytic index of 0.021 and 0.025, respectively, compared with control (0.01). Significant (P < 0.05) elevations of white blood cells, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase were noticed on the 91st day at higher doses. Generally, this study justified the traditional use of G. latifolium fruit for general body healing and maintenance of healthy living. Long term administration is safe on the haematological and biochemical systems especially at lower doses and its toxicity at higher doses is reversible. PMID:27433504

  11. Evaluation of Antioxidant, Immunomodulatory Activities, and Safety of Ethanol Extract and Fractions of Gongronema latifolium Fruit.

    PubMed

    Agwaramgbo, Amanze; Ilodigwe, Emmanuel Emeka; Ajaghaku, Daniel Lotanna; Onuorah, Maureen Ugochukwu; Mbagwu, Sonne Ikechukwu

    2014-01-01

    Gongronema latifolium fruit has wide application in ethnomedicine, especially in maintaining healthy living and general body healing. We therefore investigated the antioxidant, immunomodulatory activities, and safety of its ethanol extract and fractions. The in vitro antioxidant activities of the extract and fractions were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) test while in vivo activities were determined using carbon tetrachloride (CCL4) induced oxidative stress. Cell and humoral mediated immune responses were also evaluated together with toxicity studies. The extract, ethyl acetate, and methanol fractions showed inhibition of DPPH radical with IC50s 120, 90, and 60 μg/mL, respectively. Methanol fraction at 200 mg/kg produced significant (P < 0.05) inhibition of lipid peroxidation (MDA conc. 1.2 μmol/L) compared to control (2.8 μmol/L). Both ethyl acetate and methanol fractions at 200 mg/kg produced significant (P < 0.05) phagocytic index of 0.021 and 0.025, respectively, compared with control (0.01). Significant (P < 0.05) elevations of white blood cells, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase were noticed on the 91st day at higher doses. Generally, this study justified the traditional use of G. latifolium fruit for general body healing and maintenance of healthy living. Long term administration is safe on the haematological and biochemical systems especially at lower doses and its toxicity at higher doses is reversible. PMID:27433504

  12. Annual report to Congress. Department of Energy activities relating to the Defense Nuclear Facilities Safety Board, calendar year 2000

    SciTech Connect

    2001-03-01

    This Annual Report to the Congress describes the Department of Energy's activities in response to formal recommendations and other interactions with the Defense Nuclear Facilities Safety Board. During 2000, the Department completed its implementation and proposed closure of one Board recommendation and completed all implementation plan milestones associated with two additional Board recommendations. Also in 2000, the Department formally accepted two new Board recommendations and developed implementation plans in response to those recommendations. The Department also made significant progress with a number of broad-based safety initiatives. These include initial implementation of integrated safety management at field sites and within headquarters program offices, issuance of a nuclear safety rule, and continued progress on stabilizing excess nuclear materials to achieve significant risk reduction.

  13. [Specific features of fear conditioning expression in active and passive rabbits].

    PubMed

    2013-11-01

    The active and passive rabbits selected previously on the basis of their behavior in open field and light-dark test, were subjected to fear conditioning using pairing light (4 s) with footshocks (10 Hz, 0.5 s). Heart rate and respiration rate were measured during the classical fear conditioning. Heart rate and respiration rate decreased in response to light before footshock in case of passive-defensive reaction. There were no heart rate and respiration rate reduction in the course of the active defensive reaction. In active rabbits, as compared to passive ones, the frequency of active locomotors reactions and heart rate were higher, the decrease of respiration rate to light was observed at later stages of training, and the detected bradycardia was not stable. Thus, based upon vegetative characteristics, the active rabbits had lower level of fear than passive ones. The active or passive behavioral strategies of animals were preserved during fear conditioning.

  14. Can simple interactions capture complex features of neural activity underlying behavior in a virtual reality environment?

    NASA Astrophysics Data System (ADS)

    Meshulam, Leenoy; Gauthier, Jeffrey; Brody, Carlos; Tank, David; Bialek, William

    The complex neural interactions which are abundant in most recordings of neural activity are relatively poorly understood. A prime example of such interactions can be found in the in vivo neural activity which underlies complex behaviors of mice, imaged in brain regions such as hippocampus and parietal cortex. Experimental techniques now allow us to accurately follow these neural interactions in the simultaneous activity of large neuronal populations of awake behaving animals. Here, we demonstrate that pairwise maximum entropy models can predict a surprising number of properties of the neural activity. The models, that are constrained with activity rates and interactions between pairs of neurons, are well fit to the activity `states' in the hippocampus and cortex of mice performing cognitive tasks while navigating in a virtual reality environment.

  15. Unreviewed safety question evaluation of 100K East and 100K West in-basin fuel characterization program activities

    SciTech Connect

    Alwardt, L.D.

    1995-01-12

    The purpose of this report is to provide the basis for answers to an Unreviewed Safety Question (USQ) safety evaluation of the 105K East (KE) and 105K West (KW) in-basin activities associated with the fuel characterization program as described in the characterization shipping plan. The significant activities that are common to both 105 KE and 105 KW basins are the movement of canisters from their main basin storage locations (or potentially from the 105 KE Tech View Pit if a dump table is available) to the south loadout pit transfer channel, hydrogen generation testing in the single element fuel container, loading the single element fuel container into the shipping cask, loading of the shipping cask onto a flat-bed trailer, return of the test fuel elements or element pieces from the 327 facility, placement of the fuel elements back into Mark 2 canisters, and placement of the canisters in the main storage basin. Decapping of canisters in the south loadout pit transfer channel and re-encapsulation of canisters are activities specific to the 105 KW basin. The scope of this safety evaluation includes only those characterization fuel shipment activities performed in the 105 KE and 105 KW fuel storage basin structures up to installation of the overpack. The packaging safety evaluation report governs the shipment of the fuel elements. The K Basins Plant Review Committee has determined that the in-basin activities associated with the fuel characterization program fuel shipments are bounded by the current safety envelop and do not constitute an unreviewed safety question. This determination is documented on Attachment 1.

  16. Exploratory study to evaluate tolerability, safety, and activity of Ashwagandha (Withania somnifera) in healthy volunteers.

    PubMed

    Raut, Ashwinikumar A; Rege, Nirmala N; Tadvi, Firoz M; Solanki, Punita V; Kene, Kirti R; Shirolkar, Sudatta G; Pandey, Shefali N; Vaidya, Rama A; Vaidya, Ashok B

    2012-07-01

    Ashwagandha (Withania somnifera) (WS), a "rasayana" drug, is recommended for balavardhan and mamsavardhan. The study was intended to evaluate dose-related tolerability, safety, and activity of WS formulation in normal individuals. The design was prospective, open-labeled, variable doses in volunteers. Eighteen apparently healthy volunteers (12M:6F, age:18-30 years, and BMI: 19-30) were enrolled. After baseline investigations, they received WS capsules (Rx) (aqueous extract, 8:1) daily in two divided doses with increase in daily dosage every 10 days for 30 days (750 mg/day ×10 days, 1 000 mg/day × 10 days, 1 250 mg/day × 10 days). Volunteers were assessed for symptoms/signs, vital functions, hematological and biochemical organ function tests. Muscle activity was measured by hand grip strength, quadriceps strength, and back extensor force. Exercise tolerance was determined using cycle ergometry. Lean body weight and fat% were computed from skin fold thickness measurement. Adverse events were recorded, as volunteered by the subjects. Repeated measures ANOVA, McNemar's test, and paired t test were employed. All but one volunteer tolerated WS without any adverse event. One volunteer showed increased appetite, libido, and hallucinogenic effects with vertigo at the lowest dose and was withdrawn from study. In six subjects, improvement in quality of sleep was found. Organ function tests were in normal range before and after the intervention. Reduction in total- and LDL- cholesterol and increase of strength in muscle activity was significant. Total body fat percentage showed a reduction trend. WS, in escalated dose, was tolerated well. The formulation appeared safe and strengthened muscle activity. In view of its traditional Rasayana use, further studies are planned to evaluate potential of this drug in patients of sarcopenia. PMID:23125505

  17. The Luminous Polycyclic Aromatic Hydrocarbon Emission Features: Applications to High Redshift Galaxies and Active Galactic Nuclei

    NASA Astrophysics Data System (ADS)

    Shipley, Heath V.

    2016-01-01

    For decades, significant work has been applied to calibrating emission from the ultra-violet, nebular emission lines, far-infrared, X-ray and radio as tracers of the star-formation rate (SFR) in distant galaxies. Understanding the exact rate of star-formation and how it evolves with time and galaxy mass has deep implications for how galaxies form. The co-evolution of star-formation and supermassive black hole (SMBH) accretion is one of the key problems in galaxy formation theory. But, many of these SFR indicators are influenced by SMBH accretion in galaxies and result in unreliable SFRs. Utilizing the luminous polycyclic aromatic hydrocarbon (PAH) emission features, I provide a new robust SFR calibration using the luminosity emitted from the PAHs at 6.2μm, 7.7μm and 11.3μm to solve this. The PAH features emit strongly in the mid-infrared (mid-IR; 5-25μm) mitigating dust extinction, containing on average 5-10% of the total IR luminosity in galaxies. I use a sample of 105 star-forming galaxies covering a range of total IR luminosity, LIR = L(8-1000μm) = 109 - 1012 L⊙ and redshift 0 < z < 0.4, with mid-IR spectroscopy from the Spitzer Infrared Spectrograph (IRS), and data covering other SFR indicators (Hα emission and rest-frame 24μm continuum emission). The PAH luminosity correlates linearly with the SFR as measured by the Hα luminosity (corrected for attenuation using the mono-chromatic rest-frame 24μm emission), with a tight scatter of <0.15 dex. The scatter is comparable to that between SFRs derived from the Paα and dust-corrected Hα emission lines. We present a case study in advance of JWST, which will be capable of measuring SFRs (from 8μm rest-frame photometry, i.e. PAHs) in distant galaxies (z ≤ 2) with JWST/MIRI to SFRs as low as ~10 M⊙yr-1, because the PAH features are so bright. We use Spitzer/IRS observations of PAH features in lensed star-forming galaxies at 1 < z < 3 to demonstrate the utility of the PAHs to derive SFRs that agree with

  18. Benzenediol lactones: a class of fungal metabolites with diverse structural features and biological activities.

    PubMed

    Shen, Weiyun; Mao, Hongqiang; Huang, Qian; Dong, Jinyan

    2015-06-01

    Benzenediol lactones are a structurally variable family of fungal polyketide metabolites possessing a macrolide core structure fused into a resorcinol aromatic ring. These compounds are widespread in fungi mainly in the genera such as Aigialus, Cochliobolus, Curvularia, Fusarium, Humicola, Lasiodiplodia, Penicillium and Pochonia etc. Most of these fungal metabolites were reported to possess several interesting biological activities, such as cytotoxicities, nematicidal properties, inhibition of various kinases, receptor agonists, anti-inflammatory activities, heat shock response and immune system modulatory activities etc. This review summarizes the research on the isolation, structure elucidation, and biological activities of the benzenediol lactones, along with some available structure-activity relationships, biosynthetic studies, first syntheses, and syntheses that lead to the revision of structure or stereochemistry, published up to the year of 2014. More than 190 benzenediol lactones are described, and over 300 references cited. PMID:25559850

  19. Oxygen safety

    MedlinePlus

    COPD - oxygen safety; Chronic obstructive pulmonary disease - oxygen safety; Chronic obstructive airways disease - oxygen safety; Emphysema - oxygen safety; Heart failure - oxygen-safety; Palliative care - oxygen safety; ...

  20. Parental safety concerns and active school commute: correlates across multiple domains in the home-to-school journey

    PubMed Central

    2014-01-01

    Findings offered insights into the specific issues that drive safety concerns for elementary school children’s WTS behaviors. The observed associations between more favorable perceptions of safety and WTS provide further justification for practical intervention strategies to reduce WTS barriers that can potentially bring long-term physical activity and health benefits to school-aged children. PMID:24602213

  1. [Patient safety in management contracts].

    PubMed

    Campillo-Artero, C

    2012-01-01

    Patient safety is becoming commonplace in management contracts. Since our experience in patient safety still falls short of other clinical areas, it is advisable to review some of its characteristics in order to improve its inclusion in these contracts. In this paper opinions and recommendations concerning the design and review of contractual clauses on safety are given, as well as reflections drawn from methodological papers and informal opinions of clinicians, who are most familiar with the nuances of safe and unsafe practices. After reviewing some features of these contracts, criteria for prioritizing and including safety objectives and activities in them, and key points for their evaluation are described. The need to replace isolated activities by systemic and multifaceted ones is emphasized. Errors, limitations and improvement opportunities observed when contracts are linked to indicators, information and adverse event reporting systems are analysed. Finally, the influence of the rules of the game, and clinicians behaviour are emphasised.

  2. Physical activity advertisements that feature daily well-being improve autonomy and body image in overweight women but not men.

    PubMed

    Segar, Michelle L; Updegraff, John A; Zikmund-Fisher, Brian J; Richardson, Caroline R

    2012-01-01

    The reasons for exercising that are featured in health communications brand exercise and socialize individuals about why they should be physically active. Discovering which reasons for exercising are associated with high-quality motivation and behavioral regulation is essential to promoting physical activity and weight control that can be sustained over time. This study investigates whether framing physical activity in advertisements featuring distinct types of goals differentially influences body image and behavioral regulations based on self-determination theory among overweight and obese individuals. Using a three-arm randomized trial, overweight and obese women and men (aged 40-60 yr, n = 1690) read one of three ads framing physical activity as a way to achieve (1) better health, (2) weight loss, or (3) daily well-being. Framing effects were estimated in an ANOVA model with pairwise comparisons using the Bonferroni correction. This study showed that there are immediate framing effects on physical activity behavioral regulations and body image from reading a one-page advertisement about physical activity and that gender and BMI moderate these effects. Framing physical activity as a way to enhance daily well-being positively influenced participants' perceptions about the experience of being physically active and enhanced body image among overweight women, but not men. The experiment had less impact among the obese study participants compared to those who were overweight. These findings support a growing body of research suggesting that, compared to weight loss, framing physical activity for daily well-being is a better gain-frame message for overweight women in midlife.

  3. Physical Activity Advertisements That Feature Daily Well-Being Improve Autonomy and Body Image in Overweight Women but Not Men

    PubMed Central

    Segar, Michelle L.; Updegraff, John A.; Zikmund-Fisher, Brian J.; Richardson, Caroline R.

    2012-01-01

    The reasons for exercising that are featured in health communications brand exercise and socialize individuals about why they should be physically active. Discovering which reasons for exercising are associated with high-quality motivation and behavioral regulation is essential to promoting physical activity and weight control that can be sustained over time. This study investigates whether framing physical activity in advertisements featuring distinct types of goals differentially influences body image and behavioral regulations based on self-determination theory among overweight and obese individuals. Using a three-arm randomized trial, overweight and obese women and men (aged 40–60 yr, n = 1690) read one of three ads framing physical activity as a way to achieve (1) better health, (2) weight loss, or (3) daily well-being. Framing effects were estimated in an ANOVA model with pairwise comparisons using the Bonferroni correction. This study showed that there are immediate framing effects on physical activity behavioral regulations and body image from reading a one-page advertisement about physical activity and that gender and BMI moderate these effects. Framing physical activity as a way to enhance daily well-being positively influenced participants' perceptions about the experience of being physically active and enhanced body image among overweight women, but not men. The experiment had less impact among the obese study participants compared to those who were overweight. These findings support a growing body of research suggesting that, compared to weight loss, framing physical activity for daily well-being is a better gain-frame message for overweight women in midlife. PMID:22701782

  4. Magnetic Tilts in Sunspot Groups and Active regions in the Cycle 23 obtained from the Solar Feature Catalogues

    NASA Astrophysics Data System (ADS)

    Zharkov, S. I.; Zharkova, V. V.; Bianda, M.; Cartesi, S.

    We compare a few methods for calculation of magnetic tilts in active regions using using the automated sunspot group classification from the Solar Feature Catalogues http solar inf brad ac uk for 1996-2005 The best performing methods were applied to investigate the statistical variations of magnetic field tilt in sunspot groups and their relation to the total magnetic field contained in sunspots during different phases of the solar cycle 23 The classification results are compared with the similar research for the cycle 22 and the specifics on the cycle 23 is discussed The results are also tested against the existing solar dynamo models and their applicability for the solar activity forecast

  5. Phenomenological features of mortality and morbidity dynamics in Tomsk versus heliogeophysical activity

    NASA Astrophysics Data System (ADS)

    Borodin, A. S.; Tuzhilkin, D. A.; Gudina, M. V.; Vladimirsky, B. M.

    2015-12-01

    The influence of heliogeophysical activity on the morbidity and mortality of the population in Tomsk is studied epidemiologically on the basis of regional data. The biological effectiveness of heliogeophysical factors selected on the basis of the Karhunen-Loeve method from epidemiological data on the morbidity and mortality in Tomsk in 1990-2008 is estimated. An analysis of the impact of variations in heliogeophysical activity on morbidity and mortality (according to the International Statistical Classification of Diseases and Related Health, ICD-10) showed the existence of common factors within different nosological classes that reliably correlate with the major components of variations in characteristic indices of heliogeophysical activity.

  6. An Analysis of Activities in Saudi Arabian Middle School Science Textbooks and Workbooks for the Inclusion of Essential Features of Inquiry

    NASA Astrophysics Data System (ADS)

    Aldahmash, Abdulwali H.; Mansour, Nasser S.; Alshamrani, Saeed M.; Almohi, Saeed

    2016-01-01

    This study examines Saudi Arabian middle school science textbooks' coverage of the essential features of scientific inquiry. All activities in the middle school science textbooks and workbooks were analyzed by using the scientific inquiry `essential features' rubric. The results indicated that the essential features are included in about 59 % of the analyzed science activities. However, feature 2, `making learner give priority to evidence in responding to questions' and feature 3, `allowing learner to formulate explanations from evidence' appeared more frequently than the other three features (feature 1: engaging learner in scientifically oriented questions, feature 4: helping learner connect explanations to scientific knowledge, and feature 5: helping learner communicate and justify explanations to others), whether in the activities as a whole, or in the activities included in each of the four science domains (physical science, Earth science, life science and chemistry). These features are represented in almost all activities. This means that almost all activities in the middle school science textbooks and the workbooks include features 2 and 3. Meanwhile, the mean level of inclusion of the five essential features of scientific inquiry found in the middle school science textbooks and workbooks as a whole is 2.55. However, results found for features 1, 4, 5 and for in-level inclusion of the inquiry features in each of the science domains indicate that the inclusion of the essential inquiry features is teacher-centred. As a result, neither science textbooks nor workbooks provide students with the opportunity or encouragement to develop their inquiry skills. Consequently, the results suggest important directions for educational administrators and policy-makers in the preparation and use of science educational content.

  7. An Analysis of Activities in Saudi Arabian Middle School Science Textbooks and Workbooks for the Inclusion of Essential Features of Inquiry

    ERIC Educational Resources Information Center

    Aldahmash, Abdulwali H.; Mansour, Nasser S.; Alshamrani, Saeed M.; Almohi, Saeed

    2016-01-01

    This study examines Saudi Arabian middle school science textbooks' coverage of the essential features of scientific inquiry. All activities in the middle school science textbooks and workbooks were analyzed by using the scientific inquiry "essential features" rubric. The results indicated that the essential features are included in about…

  8. [The behaviour and respiration features in rabbits with different pattern of their open field locomotor activity].

    PubMed

    Pavlova, I V; Levshina, I P; Venetsian, G L; Shuĭkin, N N; Ziablitseva, E A

    2005-09-01

    Behavior of 19 Chinchilla rabbits was tested in the open field. By the results of the first trial, animals were divided into three groups by the type of the exploratory locomotor activity (passive--37%; active--21; and medium--42%). During the repeated open field trials, the passive rabbits increased their activity and the active ones decreased it. Correlations were found between the open field behavior and patterns of the external respiration in the absence of movements. The longest respiratory cycles, expirations and respiratory breath holdings were observed in passive rabbits. Animals with prevailing right turnings in the open field displayed longer respiratory cycles and expirations than those with prevailing left turnings. It seems that the pattern of external respiration can predict the passive defensive strategy of rabbits in negative emotional situations.

  9. Metabolites in safety testing assessment in early clinical development: a case study with a glucokinase activator.

    PubMed

    Sharma, Raman; Litchfield, John; Atkinson, Karen; Eng, Heather; Amin, Neeta B; Denney, William S; Pettersen, John C; Goosen, Theunis C; Di, Li; Lee, Esther; Pfefferkorn, Jeffrey A; Dalvie, Deepak K; Kalgutkar, Amit S

    2014-11-01

    The present article summarizes Metabolites in Safety Testing (MIST) studies on a glucokinase activator, N,N-dimethyl-5-((2-methyl-6-((5-methylpyrazin-2-yl)carbamoyl)benzofuran-4-yl)oxy)pyrimidine-2-carboxamide (PF-04937319), which is under development for the treatment of type 2 diametes mellitus. Metabolic profiling in rat, dog, and human hepatocytes revealed that PF-04937319 is metabolized via oxidative (major) and hydrolytic pathways (minor). N-Demethylation to metabolite M1 [N-methyl-5-((2-methyl-6-((5-methylpyrazin-2-yl)carbamoyl)benzofuran-4-yl)oxy)pyrimidine-2-carboxamide] was the major metabolic fate of PF-04937319 in human (but not rat or dog) hepatocytes, and was catalyzed by CYP3A and CYP2C isoforms. Qualitative examination of circulating metabolites in humans at the 100- and 300-mg doses from a 14-day multiple dose study revealed unchanged parent drug and M1 as principal components. Because M1 accounted for 65% of the drug-related material at steady state, an authentic standard was synthesized and used for comparison of steady-state exposures in humans and the 3-month safety studies in rats and dogs at the no-observed-adverse-effect level. Although circulating levels of M1 were very low in beagle dogs and female rats, adequate coverage was obtained in terms of total maximal plasma concentration (∼7.7× and 1.8×) and area under the plasma concentration-time curve (AUC; 3.6× and 0.8× AUC) relative to the 100- and 300-mg doses, respectively, in male rats. Examination of primary pharmacology revealed M1 was less potent as a glucokinase activator than the parent drug (compound PF-04937319: EC50 = 0.17 μM; M1: EC50 = 4.69 μM). Furthermore, M1 did not inhibit major human P450 enzymes (IC50 > 30 μM), and was negative in the Salmonella Ames assay, with minimal off-target pharmacology, based on CEREP broad ligand profiling. Insights gained from this analysis should lead to a more efficient and focused development plan for fulfilling MIST requirements with

  10. Safety evaluation of probiotic bifidobacteria by analysis of mucin degradation activity and translocation ability.

    PubMed

    Abe, Fumiaki; Muto, Masamichi; Yaeshima, Tomoko; Iwatsuki, Keiji; Aihara, Hiroaki; Ohashi, Yuji; Fujisawa, Tomohiko

    2010-04-01

    Although probiotic-containing nutrient formulas for infants and toddlers have become very popular, some adverse effects related to translocation of probiotic strains have been reported. We assessed the safety of probiotic bifidobacteria that have been used in clinical investigations and proven to have beneficial effects, by analyzing mucin degradation activity and translocation ability. Mucin degradation activities of three probiotic bifidobacteria strains; Bifidobacterium longum BB536, Bifidobacterium breve M-16V and Bifidobacterium infantis M-63, were evaluated by three in vitro tests comprising growth in liquid medium, SDS-PAGE analysis of degraded mucin residues, and degradation assay in Petri dish. All test strains and control type strains failed to grow in the liquid medium containing mucin as the only carbon source, although good growth was obtained from fecal sample. In the SDS-PAGE analyses of mucin residues and observation of mucinolytic zone in agar plate, the three test strains also showed no mucin degradation activity as the type strains, although fecal sample yielded positive results. In another study, a high dose of B. longum BB536 was administered orally to conventional mice to examine the translocation ability. No translocation into blood, liver, spleen, kidney and mesenteric lymph nodes was observed and no disturbance of epithelial cells and mucosal layer in the ileum, cecum and colon was detected, indicating that the test strain had no translocation ability and induced no damage to intestinal surface. These results resolve the concern about bacterial translocation when using bifidobacteria strains as probiotics, which have been tested in various clinical trials, supporting the continuous use of these probiotic strains without anxiety.

  11. Object features, object locations, and object files: which does selective attention activate and when?

    PubMed

    Lamy, D; Tsal, Y

    2000-08-01

    The representation within which attention operates was investigated in 3 experiments. The task was similar to that of R. Egly, J. Driver, and R. D. Rafal (1994). Participants had to detect the presence of a target at 1 of 4 ends of 2 shapes, differing in color and form. A precue appeared at 1 of the 4 possible corners. The 2 shapes occupied either the same or different locations in the cuing and target displays. The results showed that the cued object location was attended whether or not space was task relevant, whereas the cued object features (color and form) were attended only when these were task relevant. Moreover, when object file continuity was maintained through continuous movement, attention was found to follow the cued object file as it moved while also accruing to the cued location.

  12. Annual report to Congress: Department of Energy activities relating to the Defense Nuclear Facilities Safety Board, calendar year 1998

    SciTech Connect

    1999-02-01

    This is the ninth Annual Report to the Congress describing Department of Energy (Department) activities in response to formal recommendations and other interactions with the Defense Nuclear Facilities Safety Board (Board). The Board, an independent executive-branch agency established in 1988, provides advice and recommendations to the Secretary of energy regarding public health and safety issues at the Department`s defense nuclear facilities. The Board also reviews and evaluates the content and implementation of health and safety standards, as well as other requirements, relating to the design, construction, operation, and decommissioning of the Department`s defense nuclear facilities. The locations of the major Department facilities are provided. During 1998, Departmental activities resulted in the proposed closure of one Board recommendation. In addition, the Department has completed all implementation plan milestones associated with four other Board recommendations. Two new Board recommendations were received and accepted by the Department in 1998, and two new implementation plans are being developed to address these recommendations. The Department has also made significant progress with a number of broad-based initiatives to improve safety. These include expanded implementation of integrated safety management at field sites, a renewed effort to increase the technical capabilities of the federal workforce, and a revised plan for stabilizing excess nuclear materials to achieve significant risk reduction.

  13. MSFC Skylab airlock module, volume 2. [systems design and performance, systems support activity, and reliability and safety programs

    NASA Technical Reports Server (NTRS)

    1974-01-01

    System design and performance of the Skylab Airlock Module and Payload Shroud are presented for the communication and caution and warning systems. Crew station and storage, crew trainers, experiments, ground support equipment, and system support activities are also reviewed. Other areas documented include the reliability and safety programs, test philosophy, engineering project management, and mission operations support.

  14. [Importance of the hyperuricaemia, gout and gender nosological features in the activity of general practitioner - family doctor].

    PubMed

    Rudichenko, V M

    2012-01-01

    In this article there were analyzed gender data about features of hyperuricaemia and gout: women are much older at the onset of gout arthritis (one of main reasons, probably, makes menopause by itself), have more associated comorbid deseases as hypertension and kidney failure and drinks less alcoholic beverages. It was noticed, that typical localisation of the lesion on the first toe is less often in women, and women are more inclined to use diuretics among medical drugs. Abovementioned clinical features are of some importance for the broad activity of general practitioners - family doctors. Gender features of polyarthicular gout are not uniformed. Scientific researches confirmed possibility of the genetic basis of the uric acid metabolism, which influences some fenotypical features of the organism. Several genes are known for their influence on serum uric acid: PDZK1, GCKR, SLC2A9, ABCG2, LRRC16A, SLC17A3, SLC16A9 and SLC22A12. However, conclusions of the research works confirm the necessity of scientific clarification of the importance of different factors of gender differences.

  15. Space engine safety system

    NASA Technical Reports Server (NTRS)

    Maul, William A.; Meyer, Claudia M.

    1991-01-01

    A rocket engine safety system was designed to initiate control procedures to minimize damage to the engine or vehicle or test stand in the event of an engine failure. The features and the implementation issues associated with rocket engine safety systems are discussed, as well as the specific concerns of safety systems applied to a space-based engine and long duration space missions. Examples of safety system features and architectures are given, based on recent safety monitoring investigations conducted for the Space Shuttle Main Engine and for future liquid rocket engines. Also, the general design and implementation process for rocket engine safety systems is presented.

  16. Nonimmunoglobulin target loci of activation-induced cytidine deaminase (AID) share unique features with immunoglobulin genes

    PubMed Central

    Kato, Lucia; Begum, Nasim A.; Burroughs, A. Maxwell; Doi, Tomomitsu; Kawai, Jun; Daub, Carsten O.; Kawaguchi, Takahisa; Matsuda, Fumihiko; Hayashizaki, Yoshihide; Honjo, Tasuku

    2012-01-01

    Activation-induced cytidine deaminase (AID) is required for both somatic hypermutation and class-switch recombination in activated B cells. AID is also known to target nonimmunoglobulin genes and introduce mutations or chromosomal translocations, eventually causing tumors. To identify as-yet-unknown AID targets, we screened early AID-induced DNA breaks by using two independent genome-wide approaches. Along with known AID targets, this screen identified a set of unique genes (SNHG3, MALAT1, BCL7A, and CUX1) and confirmed that these loci accumulated mutations as frequently as Ig locus after AID activation. Moreover, these genes share three important characteristics with the Ig gene: translocations in tumors, repetitive sequences, and the epigenetic modification of chromatin by H3K4 trimethylation in the vicinity of cleavage sites. PMID:22308462

  17. Ordinary and Activated Bone Grafts: Applied Classification and the Main Features

    PubMed Central

    Deev, R. V.; Drobyshev, A. Y.; Bozo, I. Y.; Isaev, A. A.

    2015-01-01

    Bone grafts are medical devices that are in high demand in clinical practice for substitution of bone defects and recovery of atrophic bone regions. Based on the analysis of the modern groups of bone grafts, the particularities of their composition, the mechanisms of their biological effects, and their therapeutic indications, applicable classification was proposed that separates the bone substitutes into “ordinary” and “activated.” The main differential criterion is the presence of biologically active components in the material that are standardized by qualitative and quantitative parameters: growth factors, cells, or gene constructions encoding growth factors. The pronounced osteoinductive and (or) osteogenic properties of activated osteoplastic materials allow drawing upon their efficacy in the substitution of large bone defects. PMID:26649300

  18. Nonimmunoglobulin target loci of activation-induced cytidine deaminase (AID) share unique features with immunoglobulin genes.

    PubMed

    Kato, Lucia; Begum, Nasim A; Burroughs, A Maxwell; Doi, Tomomitsu; Kawai, Jun; Daub, Carsten O; Kawaguchi, Takahisa; Matsuda, Fumihiko; Hayashizaki, Yoshihide; Honjo, Tasuku

    2012-02-14

    Activation-induced cytidine deaminase (AID) is required for both somatic hypermutation and class-switch recombination in activated B cells. AID is also known to target nonimmunoglobulin genes and introduce mutations or chromosomal translocations, eventually causing tumors. To identify as-yet-unknown AID targets, we screened early AID-induced DNA breaks by using two independent genome-wide approaches. Along with known AID targets, this screen identified a set of unique genes (SNHG3, MALAT1, BCL7A, and CUX1) and confirmed that these loci accumulated mutations as frequently as Ig locus after AID activation. Moreover, these genes share three important characteristics with the Ig gene: translocations in tumors, repetitive sequences, and the epigenetic modification of chromatin by H3K4 trimethylation in the vicinity of cleavage sites.

  19. Hand Safety

    MedlinePlus

    ... en gatillo See More... Hand Anatomy Hand Safety Fireworks Safety Lawnmower Safety Snowblower safety Pumpkin Carving Gardening ... en gatillo See More... Hand Anatomy Hand Safety Fireworks Safety Lawnmower Safety Snowblower safety Pumpkin Carving Gardening ...

  20. Hand Safety

    MedlinePlus

    ... Fireworks Safety Lawnmower Safety Snowblower safety Pumpkin Carving Gardening Safety Turkey Carving Removing a Ring Español Artritis ... Fireworks Safety Lawnmower Safety Snowblower safety Pumpkin Carving Gardening Safety Turkey Carving Removing a Ring Español Artritis ...

  1. 78 FR 64538 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Safety...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-29

    ...The Department of Labor (DOL) is submitting the Mine Safety and Health Administration (MSHA) sponsored information collection request (ICR) titled, ``Safety Standards for Underground Coal Mine Ventilation--Belt Entry Used as an Intake Air Course to Ventilate Working Sections and Areas Where Mechanized Mining Equipment is Being Installed or Removed,'' to the Office of Management and Budget......

  2. 25 CFR 170.141 - What Federal funds are available for a tribe's highway safety activities?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... from the U.S. Department of Health and Human Services (HHS); (g) Indian Highway Safety Program 25 CFR... program funds under 23 U.S.C. 405; (d) Alcohol traffic safety program funds under 23 U.S.C. 408;...

  3. 75 FR 78725 - Recreational Boating Safety Projects, Programs and Activities Funded Under Provisions of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-16

    ... wide spectrum of boating safety related topics and is dedicated to reducing loss of life, injuries, and... related to carbon monoxide poisoning, propeller injury mitigation, manufacturer compliance initiatives... related to coordinating and carrying out the national recreational boating safety program. In 2005,...

  4. 77 FR 57156 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Safety...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... Register on June 1, 2012 (77 FR 32698). Interested parties are encouraged to send comments to the OMB...; Safety Standards for Roof Bolts in Metal and Nonmetal Mines and Underground Coal Mines ACTION: Notice...) sponsored information collection request (ICR) revision titled, ``Safety Standards for Roof Bolts in...

  5. 76 FR 8401 - Pipeline Safety: Agency Information Collection Activities: Notice of Request for Extension of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-14

    ... collection was published in the Federal Register on December 7, 2010 (75 FR 76077) under Docket No. PHMSA.... SUPPLEMENTARY INFORMATION: Title: Pipeline Safety: Periodic Underwater Inspections. OMB Control Number: 2137... pipeline safety regulations (49 CFR parts 190-199) require operators to conduct appropriate...

  6. 77 FR 58567 - Information Collection Activities: Well Control and Production Safety Training, Submitted for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-21

    ... of the paperwork requirements in the regulations under Subpart O, ``Well Control and Production... 250, Subpart O, Well Control and Production Safety Training. OMB Control Number: 1014-0008. Abstract... 30 CFR part 250, subpart O, Well Control and Production Safety Training. Responses are mandatory...

  7. 29 CFR 500.103 - Activities not subject to vehicle safety standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... LABOR REGULATIONS MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Motor Vehicle Safety and Insurance for Transportation of Migrant and Seasonal Agricultural Workers, Housing Safety and Health for Migrant... the Act and these regulations do not apply to the transportation of any seasonal or...

  8. Playground Safety.

    ERIC Educational Resources Information Center

    Sipes, James L.

    2000-01-01

    Discusses the issues of risk, liability, and fun when landscaping playgrounds with safety in mind. The importance of playground surfaces and several preventive measures landscapers can use to reduce the risk of injury are discussed. Concluding comments address playground design features and liability. (GR)

  9. Issues and challenges for pedestrian active safety systems based on real world accidents.

    PubMed

    Hamdane, Hédi; Serre, Thierry; Masson, Catherine; Anderson, Robert

    2015-09-01

    The purpose of this study was to analyze real crashes involving pedestrians in order to evaluate the potential effectiveness of autonomous emergency braking systems (AEB) in pedestrian protection. A sample of 100 real accident cases were reconstructed providing a comprehensive set of data describing the interaction between the vehicle, the environment and the pedestrian all along the scenario of the accident. A generic AEB system based on a camera sensor for pedestrian detection was modeled in order to identify the functionality of its different attributes in the timeline of each crash scenario. These attributes were assessed to determine their impact on pedestrian safety. The influence of the detection and the activation of the AEB system were explored by varying the field of view (FOV) of the sensor and the level of deceleration. A FOV of 35° was estimated to be required to detect and react to the majority of crash scenarios. For the reaction of a system (from hazard detection to triggering the brakes), between 0.5 and 1s appears necessary.

  10. Issues and challenges for pedestrian active safety systems based on real world accidents.

    PubMed

    Hamdane, Hédi; Serre, Thierry; Masson, Catherine; Anderson, Robert

    2015-09-01

    The purpose of this study was to analyze real crashes involving pedestrians in order to evaluate the potential effectiveness of autonomous emergency braking systems (AEB) in pedestrian protection. A sample of 100 real accident cases were reconstructed providing a comprehensive set of data describing the interaction between the vehicle, the environment and the pedestrian all along the scenario of the accident. A generic AEB system based on a camera sensor for pedestrian detection was modeled in order to identify the functionality of its different attributes in the timeline of each crash scenario. These attributes were assessed to determine their impact on pedestrian safety. The influence of the detection and the activation of the AEB system were explored by varying the field of view (FOV) of the sensor and the level of deceleration. A FOV of 35° was estimated to be required to detect and react to the majority of crash scenarios. For the reaction of a system (from hazard detection to triggering the brakes), between 0.5 and 1s appears necessary. PMID:26047007

  11. [Anti-counterfeit activities of pharmaceutical companies in Japan: for patient safety].

    PubMed

    Shofuda, Ken-ichi; Aragane, Katsumi; Igari, Yasutaka; Matsumoto, Kinya; Ito, Kazuya

    2014-01-01

    Global spread of counterfeit medicines is an imminent threat for the patients' safety. Although major targets of counterfeits are still erectile dysfunction (ED) drugs in the industrialized countries, including Japan, anti-cancer agents and some medicines for metabolic syndromes are also being counterfeited and circulated to the market mainly through the Internet. Due to the global expansion of the business, pharmaceutical companies based in Japan are suffering from the damage of counterfeits, illegal sales including diversion, and thefts, which have never been experienced in the conventional domestic market. We, pharmaceutical companies, must be responsible for the prevention of the prevalence because our mission is to deliver effective and safe medicine to patients. For this end, we are taking necessary actions including, 1. Forestalling counterfeit, falsification and illicit trade: Measures to prevent counterfeiting are taken by introducing anti-counterfeit technologies to the packaging and tablets on a risk basis. It is also important to establish supply chain security on a global scale. 2. Finding out counterfeits and cooperating crackdown: We are conducting market and internet surveillances when high risk products are sold in high risk markets. The outcome of the criminal investigation is reported to authorities and police if necessary. 3. Conducting educational campaign to medical staff or patients: For example, four companies which manufacture and sell ED drug in Japan are collaboratively continuing activities to raise the awareness of the danger of Internet purchase. To deliver effective and safe medicines stably and globally, pharmaceutical companies extend comprehensive measures against counterfeit and illicit trading.

  12. Making rainfall features fun: scientific activities for teaching children aged 5-12 years

    NASA Astrophysics Data System (ADS)

    Gires, Auguste; Muller, Catherine L.; le Gueut, Marie-Agathe; Schertzer, Daniel

    2016-05-01

    Research projects now rely on an array of different channels to increase impact, including high-level scientific output, tools, and equipment, but also communication, outreach, and educational activities. This paper focuses on education for children aged 5-12 years and presents activities that aim to help them (and their teachers) grasp some of the complex underlying issues in environmental science. More generally, it helps children to become familiarized with science and scientists, with the aim to enhance scientific culture and promote careers in this field. The activities developed are focused on rainfall: (a) designing and using a disdrometer to observe the variety of drop sizes; (b) careful recording of successive dry and rainy days and reproducing patterns using a simple model based on fractal random multiplicative cascades; and (c) collaboratively writing a children's book about rainfall. These activities are discussed in the context of current state-of-the-art pedagogical practices and goals set by project funders, especially in a European Union framework.

  13. ActiveTutor: Towards More Adaptive Features in an E-Learning Framework

    ERIC Educational Resources Information Center

    Fournier, Jean-Pierre; Sansonnet, Jean-Paul

    2008-01-01

    Purpose: This paper aims to sketch the emerging notion of auto-adaptive software when applied to e-learning software. Design/methodology/approach: The study and the implementation of the auto-adaptive architecture are based on the operational framework "ActiveTutor" that is used for teaching the topic of computer science programming in first-grade…

  14. Galactan sulfate of Grateloupia indica: Isolation, structural features and antiviral activity.

    PubMed

    Chattopadhyay, Kausik; Mateu, Cecilia G; Mandal, Pinaki; Pujol, Carlos A; Damonte, Elsa B; Ray, Bimalendu

    2007-05-01

    Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed polysaccharide fractions isolated from Grateloupia indica. The crude water extract (GiWE) as well as one fraction (F3) obtained by anion exchange chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values (0.12-1.06 microg/ml) were much lower than cytotoxic concentration 50% values (>850 microg/ml). These fractions, which were effective antiviral inhibitors if added only during the adsorption period, had very low anticoagulant activity. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Chemical, chromatographic and spectroscopic methods showed that the active polysaccharide, which has an apparent molecular mass of 60 kDa and negative specific rotation [alpha]D(32) -16 degrees (c 0.2, H2O), contains alpha-(1-->4)- and alpha-(1-->3)-linked galactopyranose residues. Sulfate groups, if present, are located mostly at C-2/6 of (1-->4)- and C-4/6 of (1-->3)-linked galactopyranosyl units, and are essential for the anti herpetic activity of this polymer. PMID:17451760

  15. Structural features and antiviral activity of sulphated fucans from the brown seaweed Cystoseira indica.

    PubMed

    Mandal, Pinaki; Mateu, Cecilia Gabriela; Chattopadhyay, Kausik; Pujol, Carlos Alberto; Damonte, Elsa Beatriz; Ray, Bimalendu

    2007-01-01

    Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we have analysed sulphated-fucan-containing fractions isolated from the brown seaweed Cystoseira indica. The crude water extract (CiWE) and the main fraction (CiF3) obtained by anion exchange chromatography had potent antiviral activity against herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) without cytotoxicity for Vero cell cultures. Furthermore, they had no direct inactivating effect on virions in a virucidal assay, and lacked anticoagulant activity. The mode of action of these compounds could be mainly ascribed to an inhibitory effect on virus adsorption. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide had an apparent molecular mass of 35 kDa and contained a backbone of alpha-(1 --> 3)-linked fucopyranosyl residues substituted at C-2 with fucopyranosyl and xylopyranosyl residues. This sulphated fucan, considered the active principle of the C. indica water extract, also contained variously linked xylose and galactose units and glucuronic acid residues. Sulphate groups, if present, are located mostly at C-4 of (1 --> 3)-linked fucopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer. PMID:17626599

  16. Metabolic Features of Protochlamydia amoebophila Elementary Bodies – A Link between Activity and Infectivity in Chlamydiae

    PubMed Central

    Watzka, Margarete; Wultsch, Anna; Tziotis, Dimitrios; Montanaro, Jacqueline; Richter, Andreas; Schmitt-Kopplin, Philippe; Horn, Matthias

    2013-01-01

    The Chlamydiae are a highly successful group of obligate intracellular bacteria, whose members are remarkably diverse, ranging from major pathogens of humans and animals to symbionts of ubiquitous protozoa. While their infective developmental stage, the elementary body (EB), has long been accepted to be completely metabolically inert, it has recently been shown to sustain some activities, including uptake of amino acids and protein biosynthesis. In the current study, we performed an in-depth characterization of the metabolic capabilities of EBs of the amoeba symbiont Protochlamydia amoebophila. A combined metabolomics approach, including fluorescence microscopy-based assays, isotope-ratio mass spectrometry (IRMS), ion cyclotron resonance Fourier transform mass spectrometry (ICR/FT-MS), and ultra-performance liquid chromatography mass spectrometry (UPLC-MS) was conducted, with a particular focus on the central carbon metabolism. In addition, the effect of nutrient deprivation on chlamydial infectivity was analyzed. Our investigations revealed that host-free P. amoebophila EBs maintain respiratory activity and metabolize D-glucose, including substrate uptake as well as host-free synthesis of labeled metabolites and release of labeled CO2 from 13C-labeled D-glucose. The pentose phosphate pathway was identified as major route of D-glucose catabolism and host-independent activity of the tricarboxylic acid (TCA) cycle was observed. Our data strongly suggest anabolic reactions in P. amoebophila EBs and demonstrate that under the applied conditions D-glucose availability is essential to sustain metabolic activity. Replacement of this substrate by L-glucose, a non-metabolizable sugar, led to a rapid decline in the number of infectious particles. Likewise, infectivity of Chlamydia trachomatis, a major human pathogen, also declined more rapidly in the absence of nutrients. Collectively, these findings demonstrate that D-glucose is utilized by P. amoebophila EBs and provide

  17. Metabolic features of Protochlamydia amoebophila elementary bodies--a link between activity and infectivity in Chlamydiae.

    PubMed

    Sixt, Barbara S; Siegl, Alexander; Müller, Constanze; Watzka, Margarete; Wultsch, Anna; Tziotis, Dimitrios; Montanaro, Jacqueline; Richter, Andreas; Schmitt-Kopplin, Philippe; Horn, Matthias

    2013-01-01

    The Chlamydiae are a highly successful group of obligate intracellular bacteria, whose members are remarkably diverse, ranging from major pathogens of humans and animals to symbionts of ubiquitous protozoa. While their infective developmental stage, the elementary body (EB), has long been accepted to be completely metabolically inert, it has recently been shown to sustain some activities, including uptake of amino acids and protein biosynthesis. In the current study, we performed an in-depth characterization of the metabolic capabilities of EBs of the amoeba symbiont Protochlamydia amoebophila. A combined metabolomics approach, including fluorescence microscopy-based assays, isotope-ratio mass spectrometry (IRMS), ion cyclotron resonance Fourier transform mass spectrometry (ICR/FT-MS), and ultra-performance liquid chromatography mass spectrometry (UPLC-MS) was conducted, with a particular focus on the central carbon metabolism. In addition, the effect of nutrient deprivation on chlamydial infectivity was analyzed. Our investigations revealed that host-free P. amoebophila EBs maintain respiratory activity and metabolize D-glucose, including substrate uptake as well as host-free synthesis of labeled metabolites and release of labeled CO2 from (13)C-labeled D-glucose. The pentose phosphate pathway was identified as major route of D-glucose catabolism and host-independent activity of the tricarboxylic acid (TCA) cycle was observed. Our data strongly suggest anabolic reactions in P. amoebophila EBs and demonstrate that under the applied conditions D-glucose availability is essential to sustain metabolic activity. Replacement of this substrate by L-glucose, a non-metabolizable sugar, led to a rapid decline in the number of infectious particles. Likewise, infectivity of Chlamydia trachomatis, a major human pathogen, also declined more rapidly in the absence of nutrients. Collectively, these findings demonstrate that D-glucose is utilized by P. amoebophila EBs and provide

  18. Distinct features of circulating microparticles and their relationship with disease activity in inflammatory bowel disease

    PubMed Central

    Voudoukis, Evangelos; Vetsika, Eleni-Kyriaki; Giannakopoulou, Konstantina; Karmiris, Konstantinos; Theodoropoulou, Angeliki; Sfiridaki, Aekaterini; Georgoulias, Vassilis; Paspatis, Gregorios A.; Koutroubakis, Ioannis E.

    2016-01-01

    Background There is evidence that circulating microparticles (MPs) and annexin (+) platelet-derived MPs (PDMPs) are increased in inflammatory bowel disease (IBD). The aim of our study was to characterize the abundance, origin, and annexin V binding of MPs in patients with IBD and correlate them with the disease characteristics. Methods Case-control study of 46 IBD patients (23 Crohn’s disease, 23 ulcerative colitis) and 40 matched healthy controls (HC). MPs were divided according to annexin V binding, their origin was estimated based on specific cell membrane markers in plasma samples and their number was calculated via flow cytometry. Clinical and laboratory activity indices were also analyzed. Results Annexin (-) PDMPs (P=0.0004), total (P=0.04) and annexin (+) monocyte-derived MPs (P=0.02) were increased and annexin (-) total MPs (P=0.0007) were decreased in IBD patients compared to HC. The annexin (+)/(-) ratio of all MP types were significantly elevated in IBD patients compared to HC (P<0.003). IBD patients with active disease displayed elevated total and annexin (+) total MPs, total, annexin (+) and (-) PDMPs compared with those in remission (P<0.05). Annexin (-) PDMPs were considerably increased in IBD patients with active compared to those with inactive disease (P=0.0013). Total and annexin (-) PDMPs were significantly correlated with most of the disease activity indices (P<0.05). Conclusion The majority of circulating MPs, their counterparts and particularly annexin (-) PDMPs are increased in active IBD patients. Annexin (+)/(-) ratio proved to be the most reliable distinctive MP index between HC and IBD patients. PMID:27065731

  19. Enhancing magnetic nanoparticle-based DNA transfection: Intracellular-active cassette features

    NASA Astrophysics Data System (ADS)

    Vernon, Matthew Martin

    Efficient plasmid DNA transfection of embryonic stem cells, mesenchymal stem cells, neural cell lines and the majority of primary cell lines is a current challenge in gene therapy research. Magnetic nanoparticle-based DNA transfection is a gene vectoring technique that is promising because it is capable of outperforming most other non-viral transfection methods in terms of both transfection efficiency and cell viability. The nature of the DNA vector implemented depends on the target cell phenotype, where the particle surface chemistry and DNA binding/unbinding kinetics of the DNA carrier molecule play a critical role in the many steps required for successful gene transfection. Accordingly, Neuromag, an iron oxide/polymer nanoparticle optimized for transfection of neural phenotypes, outperforms many other nanoparticles and lipidbased DNA carriers. Up to now, improvements to nanomagnetic transfection techniques have focused mostly on particle functionalization and transfection parameter optimization (cell confluence, growth media, serum starvation, magnet oscillation parameters, etc.). None of these parameters are capable of assisting the nuclear translocation of delivered plasmid DNA once the particle-DNA complex is released from the endosome and dissociates in the cell's cytoplasm. In this study, incorporation of a DNA targeting sequence (DTS) feature in the transfecting plasmid DNA confers improved nuclear translocation, demonstrating significant improvement in nanomagnetic transfection efficiency in differentiated SH-SY5Y neuroblastoma cells. Other parameters, such as days in vitro, are also found to play a role and represent potential targets for further optimization.

  20. Evaluation of Safety and Antioxidant Activity of Yellow Tea (Camellia sinensis) Extract for Application in Food.

    PubMed

    Kujawska, Małgorzata; Ewertowska, Małgorzata; Ignatowicz, Ewa; Adamska, Teresa; Szaefer, Hanna; Gramza-Michałowska, Anna; Korczak, Józef; Jodynis-Liebert, Jadwiga

    2016-03-01

    The article presents an evaluation of the safety of yellow tea (Camellia sinensis) extract consumption and its antioxidant activity in an animal model. Wistar rats were exposed through diet to 2, 6, and 10 g yellow tea extract/kg feed for 90 days. No signs of toxicity and no differences in mean body weight gain in the treated and control rats were recorded throughout the experiment. No statistically significant differences in hematology findings and clinical chemistry parameters were observed between controls and treated groups. Microscopic examination of tissue sections revealed no pathology attributable to yellow tea extract intake. Lipid peroxidation level in the liver was slightly increased in medium-dose males and high-dose females and decreased in two female groups receiving 2 and 6 g/kg of the extract tested. Content of carbonyl groups in protein, as well as the basal level of DNA damage, was not changed. In a majority of rats, the activity of antioxidant enzymes was increased except superoxide dismutase in high-dose groups, glutathione peroxidase in high-dose females, glutathione reductase in low- and mid-dose groups, and glutathione S-transferase in mid-dose females and high-dose males. It could be concluded that rats tolerated well dietary treatment with yellow tea extract up to 0.8 g/kg b.w./day for 90 days. Results showed that yellow tea extract at the doses tested did not demonstrate adverse effects and improved the antioxidant status in the liver of rats.

  1. The Luminous Polycyclic Aromatic Hydrocarbon Emission Features: Applications to High Redshift Galaxies and Active Galactic Nuclei

    NASA Astrophysics Data System (ADS)

    Shipley, Heath; Papovich, Casey

    2015-08-01

    We provide a new robust star-formation rate (SFR) calibration using the luminosity from polycyclic aromatic hydrogen (PAH) molecules. The PAH features emit strongly in the mid-infrared (mid-IR; 3-19μm), mitigating dust extinction, and they are very luminous, containing 5-10% of the total IR luminosity in galaxies. We derive the calibration of the PAH luminosity as a SFR indicator using a sample of 105 star-forming galaxies covering a range of total IR luminosity, LIR = L(8-1000μm) = 109 - 1012 L⊙ and redshift 0 < z < 0.6. The PAH luminosity correlates linearly with the SFR as measured by the dust-corrected Hα luminosity (using the sum of the Hα and rest-frame 24μm luminosity from Kennicutt et al. 2009), with tight scatter of ~0.15 dex, comparable to the scatter in the dust-corrected Hα SFRs and Paα SFRs. We show this relation is sensitive to galaxy metallicity, where the PAH luminosity of galaxies with Z < 0.7 Z⊙ departs from the linear SFR relationship but in a behaved manor. We derive for this a correction to galaxies below solar metallicity. As a case study for observations with JWST, we apply the PAH SFR calibration to a sample of lensed galaxies at 1 < z < 3 with Spitzer Infrared Spectrograph (IRS) data, and we demonstrate the utility of PAHs to derive SFRs as accurate as those available from any other indicator. This new SFR indicator will be useful for probing the peak of the SFR density of the universe (1 < z < 3) and for studying the coevolution of star-formation and supermassive blackhole accretion contemporaneously in a galaxy.

  2. Classic to Contemporary: Famous Artists and Activities. A Teacher's Guide Featuring 19 Famous Artists' Biographies and Enrichment/Research Activities.

    ERIC Educational Resources Information Center

    Kinghorn, Harriet; Lewis-Spicer, Lisa

    By studying the lives, important achievements, and works of famous artists, students may come to understand what those artists hoped to communicate to the world. By participating in art activities that are related to these artists' styles, students practice skills of observing, analyzing, and creating. This instructional guide provides…

  3. Let's Meet Famous Artists. A Creative Art Activity Book. A Teacher's Guide Featuring 19 Famous Artists and Art Activities.

    ERIC Educational Resources Information Center

    Kinghorn, Harriet; And Others

    By studying the lives, important achievements, and works of famous artists, students may come to understand what those artists hoped to communicate to the world. By participating in art activities that are related to these artists' styles, students practice skills of observing, analyzing, and creating. This instructional guide provides…

  4. Mining functional relationships in feature subspaces from gene expression profiles and drug activity profiles.

    PubMed

    Bao, Lei; Guo, Tao; Sun, Zhirong

    2002-04-10

    In an effort to determine putative functional relationships between gene expression patterns and drug activity patterns of 60 human cancer cell lines, a novel method was developed to discover local associations within cell line subsets. The association of drug-gene pairs is an explorative way of discovering gene markers that predict clinical tumor sensitivity to therapy. Nine drug-gene networks were discovered, as well as dozens of gene-gene and drug-drug networks. Three drug-gene networks with well studied members were discussed and the literature shows that hypothetical functional relationships exist. Therefore, this method enables the gathering of new information beyond global associations.

  5. Testing of an actively damped boring bar featuring structurally integrated PZT stack actuators

    SciTech Connect

    Redmond, J.; Barney, P.

    1998-06-01

    This paper summarizes the results of cutting tests performed using an actively damped boring bar to minimize chatter in metal cutting. A commercially available 2 inch diameter boring bar was modified to incorporate PZT stack actuators for controlling tool bending vibrations encountered during metal removal. The extensional motion of the actuators induce bending moments in the host structure through a two-point preloaded mounting scheme. Cutting tests performed at various speeds and depths of cuts on a hardened steel workpiece illustrate the bar`s effectiveness toward eliminating chatter vibrations and improving workpiece surface finish.

  6. Purification, structure features and anti-atherosclerosis activity of a Laminaria japonica polysaccharide.

    PubMed

    Peng, Fu-Hua; Zha, Xue-Qiang; Cui, Shao-Hua; Asghar, Muhammad-Naeem; Pan, Li-Hua; Wang, Jun-Hui; Luo, Jian-Ping

    2015-11-01

    A homogeneous polysaccharide (LJP12) was isolated from Laminaria japonica by diethylaminoethyl-cellulose and Sephacryl S-500 chromatography, with a molecular weight of 2.31×10(6)Da. Monosaccharide analysis showed that LJP12 was mainly composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1:0.17:1.54:2.64:0.18. For these monosaccharides, mannose was suggested to be 1,4-linked and 1,3,6-linked while glucose was linked by 1,6-glycosidic bond. The xylose, arabinose and galactose were suggested to be the terminal residues. To study the effects of LJP12 on protecting against atherosclerosis, LJP12 was administered to LDL receptor-deficient (LDLr(-/-)) mice (50, 100 and 200mg/kg/day, n=30 for each experimental group). Results showed that LJP12 exhibited the ability to inhibit high-fat-cholesterol diet (HFD)-induced formation of atherosclerotic plaques and plasma lipid levels in a dose-dependent manner. Meanwhile, both the HFD-induced systemic inflammation and local inflammation at the site of atherosclerotic lesion were significantly attenuated by LJP12, which were accompanied by the suppression of the activation of nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinases (MAPKs) signaling pathways. Taken together, we concluded that long-term oral administration of LJP12 protects against atherosclerosis in LDLr(-/-) mice via inhibiting NF-κB/MAPKs-mediated inflammatory responses.

  7. Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation.

    PubMed

    Ngo, Huu B; Lovely, Geoffrey A; Phillips, Rob; Chan, David C

    2014-01-01

    TFAM (transcription factor A, mitochondrial) is a DNA-binding protein that activates transcription at the two major promoters of mitochondrial DNA (mtDNA)--the light strand promoter (LSP) and the heavy strand promoter 1 (HSP1). Equally important, it coats and packages the mitochondrial genome. TFAM has been shown to impose a U-turn on LSP DNA; however, whether this distortion is relevant at other sites is unknown. Here we present crystal structures of TFAM bound to HSP1 and to nonspecific DNA. In both, TFAM similarly distorts the DNA into a U-turn. Yet, TFAM binds to HSP1 in the opposite orientation from LSP explaining why transcription from LSP requires DNA bending, whereas transcription at HSP1 does not. Moreover, the crystal structures reveal dimerization of DNA-bound TFAM. This dimerization is dispensable for DNA bending and transcriptional activation but is important in DNA compaction. We propose that TFAM dimerization enhances mitochondrial DNA compaction by promoting looping of the DNA.

  8. [Features of brain oscillatory activity and cardiac defense in treatment arterial hypertensives].

    PubMed

    Aftanas, L I; Brak, I V; Gilinskaia, O M; Pavlov, S V; Reva, N V

    2014-01-01

    Stress reactivity of the motivational system of defense can be assessed with the aid the cardiac defense response (CDR) - the reaction of the cardiovascular system to unexpected aversive unconditioned stimulus. The main objective of the study was revealing putative contribution of oscillatory systems of the brain into central pathogenic mechanisms of enhanced blood pressure (BP) stress-reactivity in naive patients with arterial hypertension (AH) of the 1st-2nd degrees (n = 17) and healthy control (n = 19) subjects. Using dynamic registration "beat-by-beat" arterial pressure, and oscillatory activity related EEG (64 channels) is estimated using the event-related synchronization/desynchronization (ERD/ERS). Along with abnormally high blood pressure in patients with hypertension background set significantly lower concentrations of serotonin blood platelets and increased tonic activation of the left hemisphere, reflected in the asymmetric reduction of delta- (2-4 Hz) and theta-1 (4-6 Hz) power in the central and parietal cortex in the hemisphere CDR of the patients are characterized by hyperactivity both short- and long-latency components of blood pressure. According to the dynamic analysis of the concomitant EEG, long-latency BP components may be accounted by, among other mechanisms, weakening of the descending ("top-down") inhibitory control, hypothetically implemented with the high-frequency EEG alpha (10-12 Hz) oscillations from the medial central-parietal cortex of both hemispheres of the brain. PMID:25464727

  9. Electromagnetic Safety of Spacecraft During Active Experiments with the Use of Plasma Accelerators and Ion Injectors

    NASA Astrophysics Data System (ADS)

    Plokhikh, Andrey; Popov, Garri; Shishkin, Gennady; Antropov, Nikolay; Vazhenin, Nikolay; Soganova, Galina

    Works under the development and application of stationary and pulsed plasma accelerators of charged particles conducted at the Moscow Aviation Institute and Research Institute of Applied Mechanics and Electrodynamics during over 40 years, active experiments on board meteorological rockets, artificial Earth satellites and "Mir" orbital station including [1], allowed to obtain data on the influence of pulsed and continuous plasma injection with the given parameters on the drop of energetic particles out of the radiation belts, efficiency of artificial excitation and propagation of electromagnetic waves in ELF and VLF ranges, and evolution of artificial plasma formations in different regions of ionosphere. Variation of the near-spacecraft electromagnetic environment related to the operation of plasma injectors was registered during active experiments along with the global electrodynamic processes. The measured electromagnetic fields are of rather high intensity and occupy frequency spectrum from some Hz to tens of GHz that may be of definite danger for the operation of spacecraft and its onboard systems. Analysis for the known test data is presented in the paper and methods are discussed for the diagnostics and modeling under laboratory conditions of radiative processes proceeding at the operation of plasma accelerators and ion injectors used while making active space experiments. Great attention is paid to the methodological and metrological bases for making radio measurements in vacuum chambers, design concept and hardware configuration of ground special-purpose instrumentation scientific complexes [2]. Basic requirements are formulated for the measurements and analysis of electromagnetic fields originating during the operation of plasma accelerators, including the radiative induced and conductive components inside the spacecraft, as well as the wave emission and excitation outside the spacecraft, in the ionosphere including. Measurement results for the intrinsic

  10. Interruptions of nurses' activities and patient safety: an integrative literature review1

    PubMed Central

    Monteiro, Cintia; Avelar, Ariane Ferreira Machado; Pedreira, Mavilde da Luz Gonçalves

    2015-01-01

    OBJECTIVES: to identify characteristics related to the interruption of nurses in professional practice, as well as to assess the implications of interruptions for patient safety. METHOD: integrative literature review. The following databases were searched: Pubmed/Medline, LILACS, SciELO and Cochrane Library, using the descriptors interruptions and patient safety. An initial date was not established, but the final date was December 31, 2013. A total of 29 papers met the inclusion criteria. RESULTS: all the papers included describe interruptions as a harmful factor for patient safety. Data analysis revealed three relevant categories: characteristics of interruptions, implications for patient safety, and interventions to minimize interruptions. CONCLUSION: interruptions favor the occurrence of errors in the health field. Therefore, there is a need for further studies to understand such a phenomenon and its effects on clinical practice. PMID:25806646

  11. 77 FR 28602 - Agency Information Collection Activities; Proposed Collection; Comment Request; Early Food Safety...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-15

    ... of the amino acid similarity between the new protein and known allergens and toxins; Whether the... (57 FR 22984). The guidance entitled, ``Recommendations for the Early Food Safety Evaluation of...

  12. Initial heats of H{sub 2}S adsorption on activated carbons: Effect of surface features

    SciTech Connect

    Bagreev, A.; Adib, F.; Bandosz, T.J.

    1999-11-15

    The sorption of hydrogen sulfide was studied on activated carbons of various origins by means of inverse gas chromatography at infinite dilution. The conditions of the experiment were dry and anaerobic. Prior to the experiments the surface of some carbon samples was oxidized using either nitric acid or ammonium persulfate. Then the structural parameters of carbons were evaluated from the sorption of nitrogen. From the IGC experiments at various temperatures, heats of adsorption were calculated. The results showed that the heat of H{sub 2}S adsorption under dry anaerobic conditions does not depend on surface chemistry. The dependence of the heat of adsorption on the characteristic energy of nitrogen adsorption calculated from the Dubinin-Raduskevich equation was found. This correlation can be used to predict the heat of H{sub 2}S adsorption based on the results obtained from nitrogen adsorption.

  13. Performance analysis of a semi-active railway vehicle suspension featuring MR dampers

    NASA Astrophysics Data System (ADS)

    Kim, Hwan-Choong; Choi, Seung-Bok; Lee, Gyu-Seop; An, Chae-Hun; You, Won-Hee

    2014-03-01

    This paper presents performance analysis of semi-active railway vehicle suspension system using MR damper. In order to achieve this goal, a mathematical dynamic model of railway vehicle is derived by integrating car body, bogie frame and wheel-set which can be able to represent lateral, yaw and roll motion. Based on this model, the dynamic range of MR damper at the railway secondary suspension system and design parameters of MR damper are calculated. Subsequently, control performances of railway vehicle including car body lateral motion and acceleration of MR damper are evaluated through computer simulations. Then, the MR damper is manufactured to be retrofitted with the real railway vehicle and its characteristics are experimentally measured. Experimental performance of MR damper is assessed using test rig which is composed of a car body and two bogies.

  14. Crevasse-squeeze ridge corridors: Diagnostic features of late-stage palaeo-ice stream activity

    NASA Astrophysics Data System (ADS)

    Evans, David J. A.; Storrar, Robert D.; Rea, Brice R.

    2016-04-01

    A 200-km-long and 10-km-wide linear assemblage of till-filled geometrical ridges on the bed of the Maskwa palaeo-ice stream of the late Wisconsinan southwest Laurentide Ice Sheet are interpreted as crevasse-squeeze ridges (CSR) developed during internal flow unit reorganization, immediately prior to ice stream shutdown. Ridge orientations are predominantly orientated WNW-ESE, with a subordinate WSW-ENE alignment, both indicative of ice fracture development transverse to former ice stream flow, as indicated by NNE-SSW aligned MSGL. Subglacial till injection into basal and/or full depth, mode I and II crevasses occurred at the approximate centreline of the ice stream, in response to extension and fracturing. Landform preservation indicates that this took place during the final stages of ice streaming, immediately prior to ice stream shutdown. This linear zone of ice fracturing therefore likely represents the narrowing of the fast-flowing trunk, similar to the plug flow identified in some surging valley glaciers. Lateral drag between the final active flow unit and the slower moving ice on either side is likely recorded by the up-ice bending of the CSR limbs. The resulting CSR corridor, here related to an individual ice stream flow unit, constitutes a previously unreported style of crevasse infilling and contrasts with two existing CSR patterns: (1) wide arcuate zones of CSRs related to widespread fracturing within glacier surge lobes; and (2) narrow concentric arcs of CSRs and recessional push moraines related to submarginal till deformation at active temperate glacier lobes.

  15. Can Confirmatory Biopsy be Omitted in Prostate Cancer Active Surveillance Patients with Favorable Diagnostic Features?

    PubMed Central

    Satasivam, Prassannah; Poon, Bing Ying; Ehdaie, Behfar; Vickers, Andrew J.; Eastham, James A.

    2016-01-01

    Purpose We evaluated whether initial diagnostic parameters could predict the confirmatory biopsy result in patients initiating active surveillance for prostate cancer, to determine whether some men at low risk of reclassification could be spared unnecessary biopsy. Materials and Methods The cohort included 392 men with Gleason 6 prostate cancer on initial biopsy undergoing confirmatory biopsy. We used univariate and multivariable logistic regression to assess if high-grade cancer (Gleason ≥ 7) on confirmatory biopsy could be predicted from initial diagnostic parameters (prostate-specific antigen density, magnetic resonance imaging result, percent positive cores, percent cancer in positive cores, and total tumor length). Results Median age was 62 years (IQR 56–66) and 47% of patients were found to have a dominant or focal lesion on magnetic resonance imaging. Of the 392 patients, 44 (11%) were found to have high-grade cancer on confirmatory biopsy, among whom 39 had 3+4, 1 had 4+3, 3 had Gleason 8, and 1 patient had Gleason 9 disease. All predictors were significantly associated with high-grade cancer at confirmatory biopsy on univariate analysis. However, in the multivariable model only prostate-specific antigen density and total tumor length were significantly associated (AUC of 0.85). Using this model to select patients for confirmatory biopsy would generally provide a higher net benefit than performing confirmatory biopsy in all patients, across a wide range of threshold probabilities. Conclusion If externally validated, a model based on initial diagnostic criteria could be used to avoid confirmatory biopsy in many patients initiating active surveillance. PMID:26192258

  16. [Anti-counterfeit activities of pharmaceutical companies in Japan: for patient safety].

    PubMed

    Shofuda, Ken-ichi; Aragane, Katsumi; Igari, Yasutaka; Matsumoto, Kinya; Ito, Kazuya

    2014-01-01

    Global spread of counterfeit medicines is an imminent threat for the patients' safety. Although major targets of counterfeits are still erectile dysfunction (ED) drugs in the industrialized countries, including Japan, anti-cancer agents and some medicines for metabolic syndromes are also being counterfeited and circulated to the market mainly through the Internet. Due to the global expansion of the business, pharmaceutical companies based in Japan are suffering from the damage of counterfeits, illegal sales including diversion, and thefts, which have never been experienced in the conventional domestic market. We, pharmaceutical companies, must be responsible for the prevention of the prevalence because our mission is to deliver effective and safe medicine to patients. For this end, we are taking necessary actions including, 1. Forestalling counterfeit, falsification and illicit trade: Measures to prevent counterfeiting are taken by introducing anti-counterfeit technologies to the packaging and tablets on a risk basis. It is also important to establish supply chain security on a global scale. 2. Finding out counterfeits and cooperating crackdown: We are conducting market and internet surveillances when high risk products are sold in high risk markets. The outcome of the criminal investigation is reported to authorities and police if necessary. 3. Conducting educational campaign to medical staff or patients: For example, four companies which manufacture and sell ED drug in Japan are collaboratively continuing activities to raise the awareness of the danger of Internet purchase. To deliver effective and safe medicines stably and globally, pharmaceutical companies extend comprehensive measures against counterfeit and illicit trading. PMID:24492224

  17. Maintenance: organizational modes, activities and health and safety. Use of a French national survey and in-situ analyses.

    PubMed

    Grusenmeyer, Corinne

    2014-12-01

    Maintenance activities are identified as critical both to operator safety and to systems safety and reliability. However, it is still difficult to identify maintenance workers in French occupational accident and disease statistics. Moreover, few analyses of these activities and of organizational changes in this field have been conducted. This paper presents two different approaches to this same issue. Analyses were aimed firstly at identifying the occupational exposures of these operators and at comparing them with occupational exposures of production staff and, secondly at developing understanding of normal real maintenance activities, i.e. maintenance activities that are normally actually carried out, while taking into account the socio-technical system and maintenance organization within which they lie. The use of the French SUMER 2003 survey shows that occupational exposures of maintenance staff to various constraints are more frequent than occupational exposures of their production colleagues. However, maintenance staff appear to have greater independence. Analyses were also conducted in a subcontracting urban public transport company, who outsources some maintenance work. Those analyses highlight a complex network of companies involved in maintenance activities, a substantial number of work interruptions and a significant fragmentation of the internal technicians' activities that can be cognitively costly, reduce anticipation possibilities and lead to incidents or accidents. Above all they underline internal technicians' contributions to the completion of outsourced interventions and interdependent relationships between the activities of the internal and the external technicians. Outsourcing maintenance interventions thus raises the question of risks associated with the interdependence of actual work activities undertaken by the different types of staff, since they contribute to the same maintenance intervention. This study therefore pinpoints the need to

  18. More target features in visual working memory leads to poorer search guidance: evidence from contralateral delay activity.

    PubMed

    Schmidt, Joseph; MacNamara, Annmarie; Proudfit, Greg Hajcak; Zelinsky, Gregory J

    2014-01-01

    The visual-search literature has assumed that the top-down target representation used to guide search resides in visual working memory (VWM). We directly tested this assumption using contralateral delay activity (CDA) to estimate the VWM load imposed by the target representation. In Experiment 1, observers previewed four photorealistic objects and were cued to remember the two objects appearing to the left or right of central fixation; Experiment 2 was identical except that observers previewed two photorealistic objects and were cued to remember one. CDA was measured during a delay following preview offset but before onset of a four-object search array. One of the targets was always present, and observers were asked to make an eye movement to it and press a button. We found lower magnitude CDA on trials when the initial search saccade was directed to the target (strong guidance) compared to when it was not (weak guidance). This difference also tended to be larger shortly before search-display onset and was largely unaffected by VWM item-capacity limits or number of previews. Moreover, the difference between mean strong- and weak-guidance CDA was proportional to the increase in search time between mean strong-and weak-guidance trials (as measured by time-to-target and reaction-time difference scores). Contrary to most search models, our data suggest that trials resulting in the maintenance of more target features results in poor search guidance to a target. We interpret these counterintuitive findings as evidence for strong search guidance using a small set of highly discriminative target features that remain after pruning from a larger set of features, with the load imposed on VWM varying with this feature-consolidation process. PMID:24599946

  19. More target features in visual working memory leads to poorer search guidance: evidence from contralateral delay activity.

    PubMed

    Schmidt, Joseph; MacNamara, Annmarie; Proudfit, Greg Hajcak; Zelinsky, Gregory J

    2014-03-05

    The visual-search literature has assumed that the top-down target representation used to guide search resides in visual working memory (VWM). We directly tested this assumption using contralateral delay activity (CDA) to estimate the VWM load imposed by the target representation. In Experiment 1, observers previewed four photorealistic objects and were cued to remember the two objects appearing to the left or right of central fixation; Experiment 2 was identical except that observers previewed two photorealistic objects and were cued to remember one. CDA was measured during a delay following preview offset but before onset of a four-object search array. One of the targets was always present, and observers were asked to make an eye movement to it and press a button. We found lower magnitude CDA on trials when the initial search saccade was directed to the target (strong guidance) compared to when it was not (weak guidance). This difference also tended to be larger shortly before search-display onset and was largely unaffected by VWM item-capacity limits or number of previews. Moreover, the difference between mean strong- and weak-guidance CDA was proportional to the increase in search time between mean strong-and weak-guidance trials (as measured by time-to-target and reaction-time difference scores). Contrary to most search models, our data suggest that trials resulting in the maintenance of more target features results in poor search guidance to a target. We interpret these counterintuitive findings as evidence for strong search guidance using a small set of highly discriminative target features that remain after pruning from a larger set of features, with the load imposed on VWM varying with this feature-consolidation process.

  20. Study of Fractal Features of Geomagnetic Activity Through an MHD Shell Model

    NASA Astrophysics Data System (ADS)

    Dominguez, M.; Nigro, G.; Munoz, V.; Carbone, V.

    2013-12-01

    Studies on complexity have been of great interest in plasma physics, because they provide new insights and reveal possible universalities on issues such as geomagnetic activity, turbulence in laboratory plasmas, physics of the solar wind, etc. [1, 2]. In particular, various studies have discussed the relationship between the fractal dimension, as a measure of complexity, and physical processes in magnetized plasmas such as the Sun's surface, the solar wind and the Earth's magnetosphere, including the possibility of forecasting geomagnetic activity [3, 4, 5]. Shell models are low dimensional dynamical models describing the main statistical properties of magnetohydrodynamic (MHD) turbulence [6]. These models allow us to describe extreme parameter conditions hence reaching very high Reynolds (Re) numbers. In this work a MHD shell model is used to describe the dissipative events which are taking place in the Earth's magnetosphere and causing geomagnetic storms. The box-counting fractal dimension (D) [7] is calculated for the time series of the magnetic energy dissipation rate obtained in this MHD shell model. We analyze the correlation between D and the energy dissipation rate in order to make a comparison with the same analysis made on the geomagnetic data. We show that, depending on the values of the viscosity and the diffusivity, the fractal dimension and the occurrence of bursts exhibit correlations similar as those observed in geomagnetic and solar data, [8] suggesting that the latter parameters could play a fundamental role in these processes. References [1] R. O. Dendy, S. C. Chapman, and M. Paczuski, Plasma Phys. Controlled Fusion 49, A95 (2007). [2] T. Chang and C. C. Wu, Phys. Rev. E 77, 045401 (2008). [3] R. T. J. McAteer, P. T. Gallagher, and J. Ireland, Astrophys. J. 631, 628 (2005). [4] V. M. Uritsky, A. J. Klimas, and D. Vassiliadis, Adv. Space Res. 37, 539 (2006). [5] S. C. Chapman, B. Hnat, and K. Kiyani, Nonlinear Proc. Geophys. 15, 445 (2008). [6] G

  1. Structural features and antitumor activity of a novel polysaccharide from alkaline extract of Phellinus linteus mycelia.

    PubMed

    Pei, Juan-Juan; Wang, Zhen-Bin; Ma, Hai-Le; Yan, Jing-Kun

    2015-01-22

    A novel high molecular weight polysaccharide (PL-N1) was isolated from alkaline extract of the cultured Phellinus linteus mycelia. The weight average molecular weight (Mw) of PL-N1 was estimated at 343,000kDa. PL-N1 comprised arabinose, xylose, glucose, and galactose in the molar ratio of 4.0:6.7:1.3:1.0. The chemical structure of PL-N1 was investigated by FTIR and NMR spectroscopies and methylation analysis. The results showed that the backbone of PL-N1 comprised (1→4)-linked β-D-xylopyranosyl residues, (1→2)-linked α-D-xylopyranosyl residues, (1→4)-linked α-D-glucopyranosyl residues, (1→5)-linked β-D-arabinofuranosyl residues, (1→4)-linked β-D-xylopyranosyl residues which branched at O-2, and (1→4)-linked β-D-galactopyranosyl residues which branched at O-6. The branches consisted of (1→)-linked α-D-arabinofuranosyl residues. Antitumor activity assay in vitro showed that PL-N1 could inhibit the growth of HepG2 cells to a certain extent in a dose-dependent manner. Thus, PL-N1 may be developed as a potential, natural antitumor agent and functional food.

  2. Notch is active in Langerhans cell histiocytosis and confers pathognomonic features on dendritic cells.

    PubMed

    Hutter, Caroline; Kauer, Max; Simonitsch-Klupp, Ingrid; Jug, Gunhild; Schwentner, Raphaela; Leitner, Judith; Bock, Peter; Steinberger, Peter; Bauer, Wolfgang; Carlesso, Nadia; Minkov, Milen; Gadner, Helmut; Stingl, Georg; Kovar, Heinrich; Kriehuber, Ernst

    2012-12-20

    Langerhans cell histiocytosis (LCH) is an enigmatic disease defined by the accumulation of Langerhans cell-like dendritic cells (DCs). In the present study, we demonstrate that LCH cells exhibit a unique transcription profile that separates them not only from plasmacytoid and myeloid DCs, but also from epidermal Langerhans cells, indicating a distinct DC entity. Molecular analysis revealed that isolated and tissue-bound LCH cells selectively express the Notch ligand Jagged 2 (JAG2) and are the only DCs that express both Notch ligand and its receptor. We further show that JAG2 signaling induces key LCH-cell markers in monocyte-derived DCs, suggesting a functional role of Notch signaling in LCH ontogenesis. JAG2 also induced matrix-metalloproteinases 1 and 12, which are highly expressed in LCH and may account for tissue destruction in LCH lesions. This induction was selective for DCs and was not recapitulated in monocytes. The results of the present study suggest that JAG2-mediated Notch activation confers phenotypic and functional aspects of LCH to DCs; therefore, interference with Notch signaling may be an attractive strategy to combat this disease.

  3. Structural features of Escherichia coli heat-stable enterotoxin that activates membrane-associated guanylyl cyclase.

    PubMed

    Sato, T; Shimonishi, Y

    2004-03-01

    Heat-stable enterotoxin (ST), a small peptide of 18 or 19 amino acid residues produced by enterotoxigenic Escherichia coli, is the cause of acute diarrhea in infants and travelers in developing countries. ST triggers a biological response by binding to a membrane-associated guanylyl cyclase C (GC-C) which is located on intestinal epithelial cell membranes. This binding causes an increase in the concentration of cGMP as a second messenger in cells and activates protein kinase A and cystic fibrosis transmembrane conductance regulator. Here we describe the crystal structure of an ST at 0.89 A resolution. The molecule has a ring-shaped molecular architecture consisting of six peptide molecules with external and internal diameters of approximately 35 and 7 A, respectively and a thickness of approximately 11 A. The conserved residues at the central portion of ST are distributed on the outer surface of the ring-shaped peptide hexamer, suggesting that the hexamer may be implicated in the association with GC-C through these invariant residues. PMID:15049831

  4. The structural features of hemicelluloses dissolved out at different cooking stages of active oxygen cooking process.

    PubMed

    Shi, Jianbin; Yang, Qiulin; Lin, Lu

    2014-04-15

    This work described the morphologic changes of corn stalk and the structural characterization of its hemicelluloses dissolved in yellow liquor at different cooking stages. The results showed that active oxygen cooking process was an efficient method to depolymerize the corn stalk into cellulose, hemicelluloses, and lignin as a pretreatment of biomass conversion. This cooking process can also be divided into three phases: bulk delignification, extended delignification, and residual delignification. During the heating-up period 57.67% of hemicelluloses and 62.31% of lignin were removed from the raw material. However, only 15% of hemicelluloses and 23.21% of lignin were removed during at temperature' period. The hemicelluloses from the corn stalk and yellow liquor were composed of (1→4)-β-D-xylopyranose backbones substituted with α-l-arabinofuranosyl, 4-O-methyl-α-D-glucuronic acid, and some methoxyl residues. The backbones of hemicelluloses were gradually cleaved during the cooking process. The acetyl groups substituted with xylopyranosyl residues were completely cleaved during the cooking process.

  5. Feature activated molecular dynamics: an efficient approach for atomistic simulation of solid-state aggregation phenomena.

    PubMed

    Prasad, Manish; Sinno, Talid

    2004-11-01

    An efficient approach is presented for performing efficient molecular dynamics simulations of solute aggregation in crystalline solids. The method dynamically divides the total simulation space into "active" regions centered about each minority species, in which regular molecular dynamics is performed. The number, size, and shape of these regions is updated periodically based on the distribution of solute atoms within the overall simulation cell. The remainder of the system is essentially static except for periodic rescaling of the entire simulation cell in order to balance the pressure between the isolated molecular dynamics regions. The method is shown to be accurate and robust for the Environment-Dependant Interatomic Potential (EDIP) for silicon and an Embedded Atom Method potential (EAM) for copper. Several tests are performed beginning with the diffusion of a single vacancy all the way to large-scale simulations of vacancy clustering. In both material systems, the predicted evolutions agree closely with the results of standard molecular dynamics simulations. Computationally, the method is demonstrated to scale almost linearly with the concentration of solute atoms, but is essentially independent of the total system size. This scaling behavior allows for the full dynamical simulation of aggregation under conditions that are more experimentally realizable than would be possible with standard molecular dynamics.

  6. Anthelmintic Activities against Haemonchus contortus or Trichostrongylus colubriformis from Small Ruminants Are Influenced by Structural Features of Condensed Tannins.

    PubMed

    Quijada, Jessica; Fryganas, Christos; Ropiak, Honorata M; Ramsay, Aina; Mueller-Harvey, Irene; Hoste, Hervé

    2015-07-22

    Plants containing condensed tannins (CTs) may hold promise as alternatives to synthetic anthelmintic (AH) drugs for controlling gastrointestinal nematodes (GINs). However, the structural features that contribute to the AH activities of CTs remain elusive. This study probed the relationships between CT structures and their AH activities. Eighteen plant resources were selected on the basis of their diverse CT structures. From each plant resource, two CT fractions were isolated and their in vitro AH activities were measured with the larval exsheathment inhibition assay, which was applied to Haemonchus contortus and Trichostrongylus colubriformis. Calculation of mean EC50 values indicated that H. contortus was more susceptible than T. colubriformis to the different fractions and that the F1 fractions were less efficient than the F2 ones, as indicated by the respective mean values for H. contortus, F1 = 136.9 ± 74.1 μg/mL and F2 = 108.1 ± 53.2 μg/mL, and for T. colubriformis, F1 = 233 ± 54.3 μg/mL and F2 = 166 ± 39.9 μg/mL. The results showed that the AH activity against H. contortus was associated with the monomeric subunits that give rise to prodelphinidins (P < 0.05) and with CT polymer size (P < 0.10). However, for T. colubriformis AH activity was correlated only with prodelphinidins (P < 0.05). These results suggest that CTs have different modes of action against different parasite species.

  7. Featured Article: Teriflunomide, an immunomodulatory drug, exerts anticancer activity in triple negative breast cancer cells

    PubMed Central

    Huang, Ou; Zhang, Weili; Zhi, Qiaoming; Xue, Xiaofeng; Liu, Hongchun; Shen, Daoming; Geng, Meiyu; Xie, Zuoquan

    2015-01-01

    Triple-negative breast cancer (TNBC) is defined as a group of primary breast cancers lacking expression of estrogen, progesterone, and human epidermal growth factor receptor-2 (HER-2) receptors, characterized by higher relapse rate and lower survival compared with other subtypes. Due to lack of identified targets and molecular heterogeneity, conventional chemotherapy is the only available option for treatment of TNBC, but non-discordant positive therapeutic efficacy could not be achieved. Here, we demonstrated that these TNBC cells were sensitive to teriflunomide, which was a well-known immunomodulatory drug for treatment of relapsing multiple sclerosis (MS). Potent anti-cancer effects in TNBC in vitro, including proliferation inhibition, cell cycle delay, cell apoptosis, and suppression of cell motility and invasiveness, could be achieved with this agent. Of note, we showed that multiple signals involved in TNBC proliferation, survival, migratory, and invasive potential were under regulation by teriflunomide. Among them, we identified down-regulation of growth factor receptors to abolish growth maintenance, suppression of c-Myc, and cyclin D1 to contribute to its anti-proliferative effect, modulation of components of cell cycle to induce S-phase arrest, degradation of Bcl-xL, and up-regulation of BAX via activation of MAPK pathway to induce apoptosis, and inhibition of epithelial-mesenchymal transition (EMT) process, matrix metalloproteinase-9 (MMP9) expression, and inactivation of Src/FAK to reduce TNBC migration and invasion. The results identified teriflunomide may be of therapeutic benefit for the more aggressive and difficult-to-treat breast cancer subtype, indicating the use of teriflunomide for clinical trials for treatment of TNBC patients. PMID:25304315

  8. Resistance to Degradation and Cellular Distribution are Important Features for the Antitumor Activity of Gomesin

    PubMed Central

    Buri, Marcus V.; Domingues, Tatiana M.; Paredes-Gamero, Edgar J.; Casaes-Rodrigues, Rafael L.; Rodrigues, Elaine Guadelupe; Miranda, Antonio

    2013-01-01

    Many reports have shown that antimicrobial peptides exhibit anticancer abilities. Gomesin (Gm) exhibits potent cytotoxic activity against cancer cells by a membrane pore formation induced after well-orchestrated intracellular mechanisms. In this report, the replacements of the Cys by Ser or Thr, and the use D-amino acids in the Gm structure were done to investigate the importance of the resistance to degradation of the molecule with its cytotoxicity. [Thr2,6,11,15]-Gm, and [Ser2,6,11,15]-Gm exhibits low cytotoxicity, and low resistance to degradation, and after 24 h are present in localized area near to the membrane. Conversely, the use of D-amino acids in the analogue [D-Thr2,6,11,15]-D-Gm confers resistance to degradation, increases its potency, and maintained this peptide spread in the cytosol similarly to what happens with Gm. Replacements of Cys by Thr and Gln by L- or D-Pro ([D-Thr2,6,11,15, Pro9]-D-Gm, and [Thr2,6,11,15, D-Pro9]-Gm), which induced a similar β-hairpin conformation, also increase their resistance to degradation, and cytotoxicity, but after 24 h they are not present spread in the cytosol, exhibiting lower cytotoxicity in comparison to Gm. Additionally, chloroquine, a lysosomal enzyme inhibitor potentiated the effect of the peptides. Furthermore, the binding and internalization of peptides was determined, but a direct correlation among these factors was not observed. However, cholesterol ablation, which increase fluidity of cellular membrane, also increase cytotoxicity and internalization of peptides. β-hairpin spatial conformation, and intracellular localization/target, and the capability of entry are important properties of gomesin cytotoxicity. PMID:24312251

  9. Structural Features and Chaperone Activity of the NudC Protein Family

    SciTech Connect

    Zheng, Meiying; Cierpicki, Tomasz; Burdette, Alexander J.; Utepbergenov, Darkhan; Janczyk, Pawe; #322; #321; .; Derewenda, Urszula; Stukenberg, P. Todd; Caldwell, Kim A.; Derewenda, Zygmunt S.

    2012-05-25

    The NudC family consists of four conserved proteins with representatives in all eukaryotes. The archetypal nudC gene from Aspergillus nidulans is a member of the nud gene family that is involved in the maintenance of nuclear migration. This family also includes nudF, whose human orthologue, Lis1, codes for a protein essential for brain cortex development. Three paralogues of NudC are known in vertebrates: NudC, NudC-like (NudCL), and NudC-like 2 (NudCL2). The fourth distantly related member of the family, CML66, contains a NudC-like domain. The three principal NudC proteins have no catalytic activity but appear to play as yet poorly defined roles in proliferating and dividing cells. We present crystallographic and NMR studies of the human NudC protein and discuss the results in the context of structures recently deposited by structural genomics centers (i.e., NudCL and mouse NudCL2). All proteins share the same core CS domain characteristic of proteins acting either as cochaperones of Hsp90 or as independent small heat shock proteins. However, while NudC and NudCL dimerize via an N-terminally located coiled coil, the smaller NudCL2 lacks this motif and instead dimerizes as a result of unique domain swapping. We show that NudC and NudCL, but not NudCL2, inhibit the aggregation of several target proteins, consistent with an Hsp90-independent heat shock protein function. Importantly, and in contrast to several previous reports, none of the three proteins is able to form binary complexes with Lis1. The availability of structural information will be of help in further studies on the cellular functions of the NudC family.

  10. THE DISK EVAPORATION MODEL FOR THE SPECTRAL FEATURES OF LOW-LUMINOSITY ACTIVE GALACTIC NUCLEI

    SciTech Connect

    Qiao, Erlin; Liu, B. F.; Panessa, Francesca; Liu, J. Y.

    2013-11-10

    Observations show that the accretion flows in low-luminosity active galactic nuclei probably have a two-component structure with an inner, hot, optically thin, advection-dominated accretion flow (ADAF) and an outer, truncated, cool, optically thick accretion disk. As shown by Taam et al., the truncation radius as a function of mass accretion rate is strongly affected by including the magnetic field within the framework of disk evaporation model, i.e., an increase in the magnetic field results in a smaller truncation radius of the accretion disk. In this work, we calculate the emergent spectrum of an inner ADAF + an outer truncated accretion disk around a supermassive black hole based on the prediction by Taam et al.. It is found that an increase in the magnetic field from β = 0.8 to β = 0.5 (with magnetic pressure p{sub m} = B {sup 2}/8π = (1 – β)p{sub tot}, p{sub tot} = p{sub gas} + p{sub m}) results in a factor of ∼8.7 increase in the luminosity from the truncated accretion disk. Meanwhile, results of the peak emission of the truncated accretion disk shift toward a a factor of ∼5 higher frequency. We found that the equipartition of gas pressure to magnetic pressure, i.e., β = 0.5, failed to explain the observed anti-correlation between L{sub 2-10{sub keV}}/L{sub Edd} and the bolometric correction κ{sub 2-10{sub keV}} (with κ{sub 2-10{sub keV}} = L{sub bol}/L{sub 2-10{sub keV}}). The emergent spectra for larger values of β = 0.8 or β = 0.95 can explain the observed L{sub 2-10{sub keV}}/L{sub Edd}-κ{sub 2-10{sub keV}} correlation. We argue that in the disk evaporation model, the electrons in the corona are assumed to be heated only by a transfer of energy from the ions to electrons via Coulomb collisions, which is reasonable for accretion with a lower mass accretion rate. Coulomb heating is the dominated heating mechanism for the electrons only if the magnetic field is strongly sub-equipartition, which is roughly consistent with observations.

  11. Intra-articular corticosteroids in the treatment of juvenile idiopathic arthritis: Safety, efficacy, and features affecting outcome. A comprehensive review of the literature

    PubMed Central

    Gotte, Alisa Carman

    2009-01-01

    Intra-articular corticosteroid injection (IACI) has been used in the treatment of inflammatory arthritis in adults for over fifty years. Over the last two decades, IACI has become an important tool in the management of juvenile idiopathic arthritis (JIA), particularly in the oligoarthritis subset of JIA. Many factors may affect the efficacy of this treatment modality, although the majority of evidence on this topic is anecdotal, nonconvincing, or conflicting. The review examines the rationale, efficacy, safety, and application of the use of IACI in the treatment of JIA, focusing on factors that affect the outcome following IACI.

  12. Erk1/2 activity promotes chromatin features and RNAPII phosphorylation at developmental promoters in mouse ESCs.

    PubMed

    Tee, Wee-Wei; Shen, Steven S; Oksuz, Ozgur; Narendra, Varun; Reinberg, Danny

    2014-02-13

    Erk1/2 activation contributes to mouse ES cell pluripotency. We found a direct role of Erk1/2 in modulating chromatin features required for regulated developmental gene expression. Erk2 binds to specific DNA sequence motifs typically accessed by Jarid2 and PRC2. Negating Erk1/2 activation leads to increased nucleosome occupancy and decreased occupancy of PRC2 and poised RNAPII at Erk2-PRC2-targeted developmental genes. Surprisingly, Erk2-PRC2-targeted genes are specifically devoid of TFIIH, known to phosphorylate RNA polymerase II (RNAPII) at serine-5, giving rise to its initiated form. Erk2 interacts with and phosphorylates RNAPII at its serine 5 residue, which is consistent with the presence of poised RNAPII as a function of Erk1/2 activation. These findings underscore a key role for Erk1/2 activation in promoting the primed status of developmental genes in mouse ES cells and suggest that the transcription complex at developmental genes is different than the complexes formed at other genes, offering alternative pathways of regulation.

  13. A disaggregate model for quantifying the safety effects of winter road maintenance activities at an operational level.

    PubMed

    Usman, Taimur; Fu, Liping; Miranda-Moreno, Luis F

    2012-09-01

    This research presents a disaggregated modeling approach for investigating the link between winter road collision occurrence, weather, road surface conditions, traffic exposure, temporal trends and site-specific effects. This approach is unique as it allows for quantification of the safety effects of different winter road maintenance activities at an operational level. Different collision frequency models are calibrated using hourly data collected from 31 different highway routes across Ontario, Canada. It is found that factors such as visibility, precipitation intensity, air temperature, wind speed, exposure, month of the winter season, and storm hour have statistically significant effects on winter road safety. Most importantly, road surface conditions are identified as one of the major contributing factors, representing the first contribution showing the empirical relationship between safety and road surface conditions at such a disaggregate level. The applicability of the modeling framework is demonstrated using several examples, such as quantification of the benefits of alternative maintenance operations and evaluation of the effects of different service standards using safety as a performance measure.

  14. Safety, Activity, and Immune Correlates of Anti–PD-1 Antibody in Cancer

    PubMed Central

    Topalian, Suzanne L.; Hodi, F. Stephen; Brahmer, Julie R.; Gettinger, Scott N.; Smith, David C.; McDermott, David F.; Powderly, John D.; Carvajal, Richard D.; Sosman, Jeffrey A.; Atkins, Michael B.; Leming, Philip D.; Spigel, David R.; Antonia, Scott J.; Horn, Leora; Drake, Charles G.; Pardoll, Drew M.; Chen, Lieping; Sharfman, William H.; Anders, Robert A.; Taube, Janis M.; McMiller, Tracee L.; Xu, Haiying; Korman, Alan J.; Jure-Kunkel, Maria; Agrawal, Shruti; McDonald, Daniel; Kollia, Georgia D.; Gupta, Ashok; Wigginton, Jon M.; Sznol, Mario

    2012-01-01

    Background Blockade of programmed death 1 (PD-1), an inhibitory receptor expressed by T cells, can overcome immune resistance. We assessed the antitumor activity and safety of BMS-936558, an antibody that specifically blocks PD-1. Methods We enrolled patients with advanced melanoma, non–small-cell lung cancer, castration-resistant prostate cancer, or renal-cell or colorectal cancer to receive anti–PD-1 antibody at a dose of 0.1 to 10.0 mg per kilogram of body weight every 2 weeks. Response was assessed after each 8-week treatment cycle. Patients received up to 12 cycles until disease progression or a complete response occurred. Results A total of 296 patients received treatment through February 24, 2012. Grade 3 or 4 drug-related adverse events occurred in 14% of patients; there were three deaths from pulmonary toxicity. No maximum tolerated dose was defined. Adverse events consistent with immune-related causes were observed. Among 236 patients in whom response could be evaluated, objective responses (complete or partial responses) were observed in those with non–small-cell lung cancer, melanoma, or renal-cell cancer. Cumulative response rates (all doses) were 18% among patients with non–small-cell lung cancer (14 of 76 patients), 28% among patients with melanoma (26 of 94 patients), and 27% among patients with renal-cell cancer (9 of 33 patients). Responses were durable; 20 of 31 responses lasted 1 year or more in patients with 1 year or more of follow-up. To assess the role of intratumoral PD-1 ligand (PD-L1) expression in the modulation of the PD-1–PD-L1 pathway, immunohistochemical analysis was performed on pretreatment tumor specimens obtained from 42 patients. Of 17 patients with PD-L1–negative tumors, none had an objective response; 9 of 25 patients (36%) with PD-L1–positive tumors had an objective response (P = 0.006). Conclusions Anti–PD-1 antibody produced objective responses in approximately one in four to one in five patients with non

  15. Do not hesitate to use Tversky-and other hints for successful active analogue searches with feature count descriptors.

    PubMed

    Horvath, Dragos; Marcou, Gilles; Varnek, Alexandre

    2013-07-22

    This study is an exhaustive analysis of the neighborhood behavior over a large coherent data set (ChEMBL target/ligand pairs of known Ki, for 165 targets with >50 associated ligands each). It focuses on similarity-based virtual screening (SVS) success defined by the ascertained optimality index. This is a weighted compromise between purity and retrieval rate of active hits in the neighborhood of an active query. One key issue addressed here is the impact of Tversky asymmetric weighing of query vs candidate features (represented as integer-value ISIDA colored fragment/pharmacophore triplet count descriptor vectors). The nearly a 3/4 million independent SVS runs showed that Tversky scores with a strong bias in favor of query-specific features are, by far, the most successful and the least failure-prone out of a set of nine other dissimilarity scores. These include classical Tanimoto, which failed to defend its privileged status in practical SVS applications. Tversky performance is not significantly conditioned by tuning of its bias parameter α. Both initial "guesses" of α = 0.9 and 0.7 were more successful than Tanimoto (at its turn, better than Euclid). Tversky was eventually tested in exhaustive similarity searching within the library of 1.6 M commercial + bioactive molecules at http://infochim.u-strasbg.fr/webserv/VSEngine.html , comparing favorably to Tanimoto in terms of "scaffold hopping" propensity. Therefore, it should be used at least as often as, perhaps in parallel to Tanimoto in SVS. Analysis with respect to query subclasses highlighted relationships of query complexity (simply expressed in terms of pharmacophore pattern counts) and/or target nature vs SVS success likelihood. SVS using more complex queries are more robust with respect to the choice of their operational premises (descriptors, metric). Yet, they are best handled by "pro-query" Tversky scores at α > 0.5. Among simpler queries, one may distinguish between "growable" (allowing for active

  16. MEMS Sensor Technologies for Human Centred Applications in Healthcare, Physical Activities, Safety and Environmental Sensing: A Review on Research Activities in Italy

    PubMed Central

    Ciuti, Gastone; Ricotti, Leonardo; Menciassi, Arianna; Dario, Paolo

    2015-01-01

    Over the past few decades the increased level of public awareness concerning healthcare, physical activities, safety and environmental sensing has created an emerging need for smart sensor technologies and monitoring devices able to sense, classify, and provide feedbacks to users’ health status and physical activities, as well as to evaluate environmental and safety conditions in a pervasive, accurate and reliable fashion. Monitoring and precisely quantifying users’ physical activity with inertial measurement unit-based devices, for instance, has also proven to be important in health management of patients affected by chronic diseases, e.g., Parkinson’s disease, many of which are becoming highly prevalent in Italy and in the Western world. This review paper will focus on MEMS sensor technologies developed in Italy in the last three years describing research achievements for healthcare and physical activity, safety and environmental sensing, in addition to smart systems integration. Innovative and smart integrated solutions for sensing devices, pursued and implemented in Italian research centres, will be highlighted, together with specific applications of such technologies. Finally, the paper will depict the future perspective of sensor technologies and corresponding exploitation opportunities, again with a specific focus on Italy. PMID:25808763

  17. MEMS sensor technologies for human centred applications in healthcare, physical activities, safety and environmental sensing: a review on research activities in Italy.

    PubMed

    Ciuti, Gastone; Ricotti, Leonardo; Menciassi, Arianna; Dario, Paolo

    2015-01-01

    Over the past few decades the increased level of public awareness concerning healthcare, physical activities, safety and environmental sensing has created an emerging need for smart sensor technologies and monitoring devices able to sense, classify, and provide feedbacks to users' health status and physical activities, as well as to evaluate environmental and safety conditions in a pervasive, accurate and reliable fashion. Monitoring and precisely quantifying users' physical activity with inertial measurement unit-based devices, for instance, has also proven to be important in health management of patients affected by chronic diseases, e.g., Parkinson's disease, many of which are becoming highly prevalent in Italy and in the Western world. This review paper will focus on MEMS sensor technologies developed in Italy in the last three years describing research achievements for healthcare and physical activity, safety and environmental sensing, in addition to smart systems integration. Innovative and smart integrated solutions for sensing devices, pursued and implemented in Italian research centres, will be highlighted, together with specific applications of such technologies. Finally, the paper will depict the future perspective of sensor technologies and corresponding exploitation opportunities, again with a specific focus on Italy.

  18. Preliminary Results of Ancillary Safety Analyses Supporting TREAT LEU Conversion Activities

    SciTech Connect

    Brunett, A. J.; Fei, T.; Strons, P. S.; Papadias, D. D.; Hoffman, E. A.; Kontogeorgakos, D. C.; Connaway, H. M.; Wright, A. E.

    2015-10-01

    The Transient Reactor Test Facility (TREAT), located at Idaho National Laboratory (INL), is a test facility designed to evaluate the performance of reactor fuels and materials under transient accident conditions. The facility, an air-cooled, graphite-moderated reactor designed to utilize fuel containing high-enriched uranium (HEU), has been in non-operational standby status since 1994. Currently, in support of the missions of the Department of Energy (DOE) National Nuclear Security Administration (NNSA) Material Management and Minimization (M3) Reactor Conversion Program, a new core design is being developed for TREAT that will utilize low-enriched uranium (LEU). The primary objective of this conversion effort is to design an LEU core that is capable of meeting the performance characteristics of the existing HEU core. Minimal, if any, changes are anticipated for the supporting systems (e.g. reactor trip system, filtration/cooling system, etc.); therefore, the LEU core must also be able to function with the existing supporting systems, and must also satisfy acceptable safety limits. In support of the LEU conversion effort, a range of ancillary safety analyses are required to evaluate the LEU core operation relative to that of the existing facility. These analyses cover neutronics, shielding, and thermal hydraulic topics that have been identified as having the potential to have reduced safety margins due to conversion to LEU fuel, or are required to support the required safety analyses documentation. The majority of these ancillary tasks have been identified in [1] and [2]. The purpose of this report is to document the ancillary safety analyses that have been performed at Argonne National Laboratory during the early stages of the LEU design effort, and to describe ongoing and anticipated analyses. For all analyses presented in this report, methodologies are utilized that are consistent with, or improved from, those used in analyses for the HEU Final Safety Analysis

  19. Classroom Activities in School Bus and Pedestrian Safety Education. Bulletin No. 93138.

    ERIC Educational Resources Information Center

    Wisconsin State Dept. of Transportation, Madison.

    School bus and related pedestrian safety education is prevention-oriented so that students will learn how to avoid bus-related accidents. This manual provides lesson plans emphasizing the school bus stop, loading and unloading zones, emergency evacuation drills, and appropriate behavior on the school bus. The guide also recognizes demographic…

  20. 75 FR 42818 - Agency Information Collection; Activity Under OMB Review; Collection of Safety Culture Data for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... on March 12, 2010 (75 FR 11988) and the comment period ended on May 11, 2010. The 60-day notice...; Collection of Safety Culture Data for Program Evaluation AGENCY: Research & Innovative Technology... Culture Data for Program Evaluation. Type of Request: Approval of a new information collection....

  1. 77 FR 28669 - Pipeline Safety: Information Collection Activities, Excess Flow Valve Census

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-15

    ..., Excess Flow Valve Census AGENCY: Pipeline and Hazardous Materials Safety Administration (PHMSA), DOT..., PHMSA invites comments on a new one-time Information Collection (IC) on Excess Flow Valves (EFVs). PHMSA... Collection on Excess Flow Valves.'' In 1996, PHMSA's predecessor agency, the Research and Special...

  2. 75 FR 35286 - Agency Information Collection Activities; Proposed Collection; Comment Request; Safety Standard...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... estimates for the marking and instructional literature requirements in the Safety Standard for Infant... instructional literature. We estimate the burden of this collection of information as follows: Table 1... of ASTM F 997-07 contain requirements for marking and instructional literature that are...

  3. 75 FR 30783 - Agency Information Collection Activities; Proposed Collection; Comment Request; Safety Standard...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ... on the burden estimates for the marking and instructional literature requirements in the Safety... requirements for marking and instructional literature. We estimate the burden of this collection of information... marking and instructional literature that are disclosure requirements, thus falling within the...

  4. 76 FR 67201 - Information Collection Activities: Oil and Gas Production Safety Systems; Submitted for Office of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... Production Safety Systems; Submitted for Office of Management and Budget (OMB) Review; Comment Request ACTION... system in lieu of a water system. BSEE may require additional information be submitted to maintain approval. The information is used to determine if the chemical-only system provides the...

  5. 75 FR 27734 - Agency Information Collection Activities; Proposed Collection; Comment Request; Safety Standard...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ... manufacture or import bicycle helmets subject to the standard. There are an estimated 200 different models of... for Bicycle Helmets AGENCY: Consumer Product Safety Commission. ACTION: Notice. SUMMARY: The Consumer... of bicycle helmets. DATES: Submit written or electronic comments on the collection of information...

  6. 29 CFR 500.103 - Activities not subject to vehicle safety standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) Carpooling. Vehicle safety standards or insurance requirements of the Act and these regulations do not apply to carpooling arrangements made by the workers themselves, using one of the workers' own vehicles and.... Carpooling, however, does not include any transportation arrangement in which a farm labor...

  7. 29 CFR 500.103 - Activities not subject to vehicle safety standards.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) Carpooling. Vehicle safety standards or insurance requirements of the Act and these regulations do not apply to carpooling arrangements made by the workers themselves, using one of the workers' own vehicles and.... Carpooling, however, does not include any transportation arrangement in which a farm labor...

  8. 29 CFR 500.103 - Activities not subject to vehicle safety standards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) Carpooling. Vehicle safety standards or insurance requirements of the Act and these regulations do not apply to carpooling arrangements made by the workers themselves, using one of the workers' own vehicles and.... Carpooling, however, does not include any transportation arrangement in which a farm labor...

  9. 29 CFR 500.103 - Activities not subject to vehicle safety standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) Carpooling. Vehicle safety standards or insurance requirements of the Act and these regulations do not apply to carpooling arrangements made by the workers themselves, using one of the workers' own vehicles and.... Carpooling, however, does not include any transportation arrangement in which a farm labor...

  10. 78 FR 48890 - Information Collection Activities: Safety and Environmental Management Systems (SEMS); Proposed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-12

    ... offshore drilling unit (MODU) and who has the final word during an emergency event. Self-audits are... and enforcement, some provisions apply to offshore operations. For example, section 108 of FOGRMA, 30... operator accountable for the overall safety of the offshore facility, including ensuring that...

  11. Instructional Resources Monograph Series: Safety in Wastewater Treatment Systems. Selected Instructional Activities and References.

    ERIC Educational Resources Information Center

    Coon, Herbert L.

    Described are instructional and reference materials that may be useful to managers, supervisors, foremen and others who are interested in the safety education of workers in wastewater systems. Emphasis is upon items relevant to the development and presentation of wastewater treatment training programs. Part I contains descriptions and excerpts…

  12. 75 FR 27731 - Agency Information Collection Activities; Proposed Collection; Comment Request; Safety Standard...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ... for Multi-Purpose Lighters AGENCY: Consumer Product Safety Commission. ACTION: Notice. SUMMARY: The... manufacturers and importers of multi-purpose lighters. DATES: Submit written or electronic comments on the...: Written comments should be captioned ``Proposed Collection of Information--Multi-Purpose Lighters'' and...

  13. Effect of dexamethasone on bactericidal activity of turkey monocytes and implications for food safety

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Stress has been shown to affect the immune system of turkeys making them more susceptible to bacterial infections that may compromise food safety. Female turkeys are more resistant to stress-induced opportunistic bacterial infections than are male turkeys. In order to determine the mechanism of this...

  14. 78 FR 41829 - Pipeline Safety: Information Collection Activities, Revision to Annual Report for Hazardous...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-11

    ... the information collection was published on February 6, 2013, (78 FR 8699). PHMSA received one comment..., Revision to Annual Report for Hazardous Liquid Pipeline Systems AGENCY: Pipeline and Hazardous Materials... from hazardous liquid operators' annual reports is an important tool for identifying safety trends...

  15. Missouri Elementary Science Safety Manual.

    ERIC Educational Resources Information Center

    Lemons, Judith L.

    The purpose of this safety manual is to provide a resource to help manage and minimize potential risks in science classrooms where students spend up to 60% of instructional time engaged in hands-on activities. Information on general laboratory safety, science equipment safety, safety with plants, safety with animals, safety with chemicals, field…

  16. Change in hippocampal theta activity with transfer from simple discrimination tasks to a simultaneous feature-negative task

    PubMed Central

    Sakimoto, Yuya; Sakata, Shogo

    2014-01-01

    It was showed that solving a simple discrimination task (A+, B−) and a simultaneous feature-negative (FN) task (A+, AB−) used the hippocampal-independent strategy. Recently, we showed that the number of sessions required for a rat to completely learn a task differed between the FN and simple discrimination tasks, and there was a difference in hippocampal theta activity between these tasks. These results suggested that solving the FN task relied on a different strategy than the simple discrimination task. In this study, we provided supportive evidence that solving the FN and simple discrimination tasks involved different strategies by examining changes in performance and hippocampal theta activity in the FN task after transfer from the simple discrimination task (A+, B− → A+, AB−). The results of this study showed that performance on the FN task was impaired and there was a difference in hippocampal theta activity between the simple discrimination task and FN task. Thus, we concluded that solving the FN task uses a different strategy than the simple discrimination task. PMID:24917797

  17. Clonal B cells in Waldenström's macroglobulinemia exhibit functional features of chronic active B-cell receptor signaling

    PubMed Central

    Argyropoulos, K V; Vogel, R; Ziegler, C; Altan-Bonnet, G; Velardi, E; Calafiore, M; Dogan, A; Arcila, M; Patel, M; Knapp, K; Mallek, C; Hunter, Z R; Treon, S P; van den Brink, M R M; Palomba, M L

    2016-01-01

    Waldenström's macroglobulinemia (WM) is a B-cell non-Hodgkin's lymphoma (B-NHL) characterized by immunoglobulin M (IgM) monoclonal gammopathy and the medullary expansion of clonal lymphoplasmacytic cells. Neoplastic transformation has been partially attributed to hyperactive MYD88 signaling, secondary to the MYD88 L265P mutation, occurring in the majority of WM patients. Nevertheless, the presence of chronic active B-cell receptor (BCR) signaling, a feature of multiple IgM+ B-NHL, remains a subject of speculation in WM. Here, we interrogated the BCR signaling capacity of primary WM cells by utilizing multiparametric phosphoflow cytometry and found heightened basal phosphorylation of BCR-related signaling proteins, and augmented phosphoresponses on surface IgM (sIgM) crosslinking, compared with normal B cells. In support of those findings we observed high sIgM expression and loss of phosphatase activity in WM cells, which could both lead to signaling potentiation in clonal cells. Finally, led by the high-signaling heterogeneity among WM samples, we generated patient-specific phosphosignatures, which subclassified patients into a ‘high' and a ‘healthy-like' signaling group, with the second corresponding to patients with a more indolent clinical phenotype. These findings support the presence of chronic active BCR signaling in WM while providing a link between differential BCR signaling utilization and distinct clinical WM subgroups. PMID:26867669

  18. Inherent design features of the GCFR

    SciTech Connect

    Medwid, W.; Breher, W.; Shenoy, A.; Elliott, R.

    1980-05-01

    This paper discusses several inherent design features of the GCFR that enhance its safety and presents analyses to demonstrate the degree of protection they provide. These features are a subset of a larger group of potential inherent features that form the third line of protection (LOP-3) for the GCFR. The function of LOP-3 is to demonstrate that the inherent response of the reactor system will limit core damage even if active cooling and shutdown systems in LOP-1 and LOP-2 fail. By providing this function with inherent features, which do not depend on active components and are self-controlling, an additional level of protection against common cause failure mechanisms is provided for both protected and unprotected events. The examples of LOP-3 discussed in this paper are natural circulation core cooling to the ultimate atmospheric heat sink and inherent reactor shutdown mechanisms.

  19. Wellness Partners: Design and Evaluation of a Web-Based Physical Activity Diary with Social Gaming Features for Adults

    PubMed Central

    Wang, Hua; Spruijt-Metz, Donna; Jordan-Marsh, Maryalice; Valente, Thomas William

    2013-01-01

    Background The United States is currently in an age of obesity and inactivity despite increasing public awareness and scientific knowledge of detrimental long-term health effects of this lifestyle. Behavior-tracking diaries offer an effective strategy for physical activity adherence and weight management. Furthermore, Web-based physical activity diaries can engage meaningful partners in people’s social networks through fun online gaming interactions and generate motivational mechanisms for effective behavioral change and positive health outcomes. Objective Wellness Partners (WP) is a Web-based intervention in the form of a physical activity diary with social networking and game features. Two versions were designed and developed for the purpose of this study—“Diary” only and “Diary+Game”. The objectives of this study included pilot testing the research process of this intervention design, implementation, evaluation, and exploring the effectiveness of social gaming features on adult participants’ physical activity and anthropometric measures. Methods We conducted a field experiment with randomized crossover design. Assessments occurred at baseline, first follow-up (FU, 5-8 weeks after using one version of WP), and second FU (5-8 weeks of using the other version of WP). In the control condition, participants started with the “Diary” version of WP while in the experimental condition, participants started with the “Diary+Game” version of WP. A total of 54 adults (egos) ages 44-88, and their family and friends (alters) ages 17-69 participated in the study in ego-network groups. Both egos and their alters completed online surveys about their exercise habits. In addition, egos completed anthropometric measurements of BMI, fat percentage, and fat mass by bioimpedance. Results From October 2009 to May 2010, flyers, emails, and Web advertisements yielded 335 volunteers who were screened. Rolling recruitment resulted in enrollment of 142 qualified

  20. A Vehicle Active Safety Model: Vehicle Speed Control Based on Driver Vigilance Detection Using Wearable EEG and Sparse Representation.

    PubMed

    Zhang, Zutao; Luo, Dianyuan; Rasim, Yagubov; Li, Yanjun; Meng, Guanjun; Xu, Jian; Wang, Chunbai

    2016-01-01

    In this paper, we present a vehicle active safety model for vehicle speed control based on driver vigilance detection using low-cost, comfortable, wearable electroencephalographic (EEG) sensors and sparse representation. The proposed system consists of three main steps, namely wireless wearable EEG collection, driver vigilance detection, and vehicle speed control strategy. First of all, a homemade low-cost comfortable wearable brain-computer interface (BCI) system with eight channels is designed for collecting the driver's EEG signal. Second, wavelet de-noising and down-sample algorithms are utilized to enhance the quality of EEG data, and Fast Fourier Transformation (FFT) is adopted to extract the EEG power spectrum density (PSD). In this step, sparse representation classification combined with k-singular value decomposition (KSVD) is firstly introduced in PSD to estimate the driver's vigilance level. Finally, a novel safety strategy of vehicle speed control, which controls the electronic throttle opening and automatic braking after driver fatigue detection using the above method, is presented to avoid serious collisions and traffic accidents. The simulation and practical testing results demonstrate the feasibility of the vehicle active safety model. PMID:26907278

  1. A Vehicle Active Safety Model: Vehicle Speed Control Based on Driver Vigilance Detection Using Wearable EEG and Sparse Representation

    PubMed Central

    Zhang, Zutao; Luo, Dianyuan; Rasim, Yagubov; Li, Yanjun; Meng, Guanjun; Xu, Jian; Wang, Chunbai

    2016-01-01

    In this paper, we present a vehicle active safety model for vehicle speed control based on driver vigilance detection using low-cost, comfortable, wearable electroencephalographic (EEG) sensors and sparse representation. The proposed system consists of three main steps, namely wireless wearable EEG collection, driver vigilance detection, and vehicle speed control strategy. First of all, a homemade low-cost comfortable wearable brain-computer interface (BCI) system with eight channels is designed for collecting the driver’s EEG signal. Second, wavelet de-noising and down-sample algorithms are utilized to enhance the quality of EEG data, and Fast Fourier Transformation (FFT) is adopted to extract the EEG power spectrum density (PSD). In this step, sparse representation classification combined with k-singular value decomposition (KSVD) is firstly introduced in PSD to estimate the driver’s vigilance level . Finally, a novel safety strategy of vehicle speed control, which controls the electronic throttle opening and automatic braking after driver fatigue detection using the above method, is presented to avoid serious collisions and traffic accidents. The simulation and practical testing results demonstrate the feasibility of the vehicle active safety model. PMID:26907278

  2. Escalator design features evaluation

    NASA Technical Reports Server (NTRS)

    Zimmerman, W. F.; Deshpande, G. K.

    1982-01-01

    Escalators are available with design features such as dual speed (90 and 120 fpm), mat operation and flat steps. These design features were evaluated based on the impact of each on capital and operating costs, traffic flow, and safety. A human factors engineering model was developed to analyze the need for flat steps at various speeds. Mat operation of escalators was found to be cost effective in terms of energy savings. Dual speed operation of escalators with the higher speed used during peak hours allows for efficient operation. A minimum number of flat steps required as a function of escalator speed was developed to ensure safety for the elderly.

  3. Application of viability theory for road vehicle active safety during cornering manoeuvres

    NASA Astrophysics Data System (ADS)

    Vandanjon, P.-O.; Coiret, A.; Lorino, T.

    2014-02-01

    Viability theory proposes geometric metaphors in addition to classical ordinary differential equation analysis. In this paper, advantages of applying viability theory to road safety domain are presented. The exact issue is to determine if, from an initial state of a vehicle/road/driver system, a soft controls strategy is compatible with a safe driving sequence. The case of a car negotiating a curve is considered. The application of the viability theory to this issue offers the advantage to avoid classical full computing of the system. Instead of that, it consists on verifying that the states and the controls belong to a subset called the viability kernel. The construction and the use of the viability kernel for a vehicle system dynamic is proposed by using support vector machines algorithm. Then, the applicability of this theory is demonstrated through experimental tests. This innovative application of the viability theory to vehicle dynamics with road safety concerns could benefit to robust embedded warning systems.

  4. Selection of orally active antifungal agents from 3,5-substituted isoxazolidine derivatives based on acute efficacy-safety profiles.

    PubMed

    Palmer, G C; Ordy, M J; Simmons, R D; Strand, J C; Radov, L A; Mullen, G B; Kinsolving, C R; St Georgiev, V; Mitchell, J T; Allen, S D

    1989-06-01

    Routine in vitro screening of a new synthetic series of 3,5-substituted 2-methylisoxazolidines revealed that three imidazole analogs (PR 967-248, PR 967-234, and PR 969-566) and, to a lesser extent, a triazole analog (PR 988-399) exerted rather potent antifungal activity against three systemic and four dermatophytic classes of fungi. When tested in vivo for ability to eradicate Candida vaginitis in the rat, the triazole derivative, PR 988-399, was effective after oral administration. In this in vivo test for efficacy, PR 967-234 and PR 969-566 reduced but did not eradicate the infection, while PR 967-248 was inactive. PR 988-399 was, moreover, 4- to 13-fold less potent than the three imidazoles in inhibiting testosterone synthesis in isolated rat Leydig cells. After oral or intravenous administration, PR 988-399 and PR 969-566 elicited the fewest cardiovascular and behavioural side effects in conscious dogs. The rat safety study consisted of oral dosing followed by evaluation of the exploratory motor activity of the naive animals in a novel environment. Motor activity was suppressed least by PR 988-399 and most by PR 969-566. In a battery of mouse behavioural-neuromuscular-drug interaction tests, PR 988-399 and PR 969-566 produced the fewest central-behavioural-neuromuscular signs. These efficacy-safety evaluations were performed with ketoconazole as a positive reference standard. The sequence of drug testing with respect to efficacy-safety considerations appears to be a suitable approach for early detection of orally active antifungal agents such as PR 988-399 for more advanced development.

  5. Do the Features, Amenities, and Quality of Physical Activity Resources Differ Between City and County Areas of a Large Rural Region?

    PubMed

    Hill, Jennie L; Waters, Clarice N; Kolivras, Korine N; Estabrooks, Paul A; Zoellner, Jamie M

    2016-01-01

    The goal of this work was to provide a community-academic partnership with actionable information for physical activity (PA) for a rural health-disparate region. Identified PA outlets were audited and combined with survey data for 813 residents in the region. Less than a third of sampled residents (28%) met PA recommendations, with low perceptions of safety for PA. PA resource outlets in rural areas had higher numbers of incivilities. On the basis of our findings, 4 actionable strategies are recommended: shared-use agreements, reducing incivilities, addressing issues related to safety from traffic, and increasing the number of PA resources in rural areas. PMID:27536932

  6. Do the Features, Amenities, and Quality of Physical Activity Resources Differ Between City and County Areas of a Large Rural Region?

    PubMed

    Hill, Jennie L; Waters, Clarice N; Kolivras, Korine N; Estabrooks, Paul A; Zoellner, Jamie M

    2016-01-01

    The goal of this work was to provide a community-academic partnership with actionable information for physical activity (PA) for a rural health-disparate region. Identified PA outlets were audited and combined with survey data for 813 residents in the region. Less than a third of sampled residents (28%) met PA recommendations, with low perceptions of safety for PA. PA resource outlets in rural areas had higher numbers of incivilities. On the basis of our findings, 4 actionable strategies are recommended: shared-use agreements, reducing incivilities, addressing issues related to safety from traffic, and increasing the number of PA resources in rural areas.

  7. A randomized, pilot study to assess the efficacy and safety of curcumin in patients with active rheumatoid arthritis.

    PubMed

    Chandran, Binu; Goel, Ajay

    2012-11-01

    Curcumin is known to possess potent antiinflammatory and antiarthritic properties. This pilot clinical study evaluated the safety and effectiveness of curcumin alone, and in combination with diclofenac sodium in patients with active rheumatoid arthritis (RA). Forty-five patients diagnosed with RA were randomized into three groups with patients receiving curcumin (500 mg) and diclofenac sodium (50 mg) alone or their combination. The primary endpoints were reduction in Disease Activity Score (DAS) 28. The secondary endpoints included American College of Rheumatology (ACR) criteria for reduction in tenderness and swelling of joint scores. Patients in all three treatment groups showed statistically significant changes in their DAS scores. Interestingly, the curcumin group showed the highest percentage of improvement in overall DAS and ACR scores (ACR 20, 50 and 70) and these scores were significantly better than the patients in the diclofenac sodium group. More importantly, curcumin treatment was found to be safe and did not relate with any adverse events. Our study provides the first evidence for the safety and superiority of curcumin treatment in patients with active RA, and highlights the need for future large-scale trials to validate these findings in patients with RA and other arthritic conditions. PMID:22407780

  8. Student research activities in the Technology Assessments Section of the Health and Safety Research Division, Summer 1980

    SciTech Connect

    Chester, R.O.; Roberts, D.A.

    1981-08-01

    Reports summarizing activities of students assigned to the Technology Assessments Section of the Health and Safety Research Division for the summer 1980 are presented. Unless indicated otherwise, each report was written by the student whose work is being described. For each student, the student's supervisor, the name of the program under which the student was brought to ORNL, the academic level of the student, and the name of the ORNL project to which the student was assigned are tabulated. The reports are presented in alphabetical order of the students' last names.

  9. Six Tips for College Health and Safety

    MedlinePlus

    ... Stages & Populations Travelers' Health Workplace Safety & Health Features Media Sign up for Features Get Email Updates To ... Report on Campus Sexual Assault [887 KB] Features Media Sign up for Features Get Email Updates To ...

  10. Neighbourhoods for Active Kids: study protocol for a cross-sectional examination of neighbourhood features and children's physical activity, active travel, independent mobility and body size

    PubMed Central

    Oliver, Melody; McPhee, Julia; Carroll, Penelope; Ikeda, Erika; Mavoa, Suzanne; Mackay, Lisa; Kearns, Robin A; Kyttä, Marketta; Asiasiga, Lanuola; Garrett, Nicholas; Lin, Judy; Mackett, Roger; Zinn, Caryn; Moewaka Barnes, Helen; Egli, Victoria; Prendergast, Kate; Witten, Karen

    2016-01-01

    Introduction New Zealand children's physical activity, including independent mobility and active travel, has declined markedly over recent decades. The Neighbourhoods for Active Kids (NfAK) study examines how neighbourhood built environments are associated with the independent mobility, active travel, physical activity and neighbourhood experiences of children aged 9–12 years in primary and intermediate schools across Auckland, New Zealand's largest city. Methods and analysis Child-specific indices of walkability, destination accessibility and traffic exposure will be constructed to measure the built environment in 8 neighbourhoods in Auckland. Interactive online-mapping software will be used to measure children's independent mobility and transport mode to destinations and to derive measures of neighbourhood use and perceptions. Physical activity will be measured using 7-day accelerometry. Height, weight and waist circumference will be objectively measured. Parent telephone interviews will collect sociodemographic information and parent neighbourhood perceptions. Interviews with school representative will capture supports and barriers for healthy activity and nutrition behaviours at the school level. Multilevel modelling approaches will be used to understand how differing built environment variables are associated with activity, neighbourhood experiences and health outcomes. Discussion We anticipate that children who reside in neighbourhoods considered highly walkable will be more physically active, accumulate more independent mobility and active travel, and be more likely to have a healthy body size. This research is timely as cities throughout New Zealand develop and implement plans to improve the liveability of intensifying urban neighbourhoods. Results will be disseminated to participants, local government agencies and through conventional academic avenues. PMID:27531740

  11. 75 FR 25282 - Office of the Director, Office of Biotechnology Activities; Notice of a Safety Symposium

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ... HUMAN SERVICES National Institutes of Health Office of the Director, Office of Biotechnology Activities.... Chezelle George, Administrative Assistant, Office of Biotechnology Activities, Office of the Director... a.m. to 5:30 p.m. Agenda: The Office of Biotechnology Activities (OBA) and NIH Recombinant...

  12. Synthesis of the quaternary in the almeria littoral neotectonic activity and its morphologic features, western betics, Spain

    NASA Astrophysics Data System (ADS)

    Goy, J. L.; Zazo, C.

    1986-11-01

    A sequence of marine and continental Quaternary episodes is established, as are their interrelationships. The zone's tectonics have, in most cases, remained active to the present and control the factors All the Tyrrhenian levels containing Strombus bubonius are affected by the many faults that cross the lateral fringe and continue onto the shelf. Among these are: the Loma del Viento Fault, (N120°E. a normal fault with certain leftward characteristics): the El Alquian faults (N140°, 160°E, which coincide, according to several authors (Bousquet, 1979), with the surface feature of a great dextral deep accident); and the Carboneras Fault, or the Serrata Fault, (N40°, 45°E, left strike-slip fault). Given the varying behaviour of the shoreline, we have chosen to divide it into four sectors: Campo de Dalias, El-Alquian-Cabo de Gata, Mojacar—Garrucha. and Campo de Nijar-Serrata. Due to its geographical location, no marine-continental relationships exist in the latter. A chart has been made in an attempt to synthesize the most significant stratigraphic, genetic and tectonic characteristics of each sector. Lastly, the influence of tectonics on the distribution and heights of the Quaternary shorelines is clearly shown in a chart of the transgressive maxima of the Tyrrhenian episodes.

  13. Evaluation of image features and search strategies for segmentation of bone structures in radiographs using Active Shape Models.

    PubMed

    Behiels, Gert; Maes, Frederik; Vandermeulen, Dirk; Suetens, Paul

    2002-03-01

    In this paper, we evaluate various image features and different search strategies for fitting Active Shape Models (ASM) to bone object boundaries in digitized radiographs. The original ASM method iteratively refines the pose and shape parameters of the point distribution model driving the ASM by a least squares fit of the shape to update the target points at the estimated object boundary position, as determined by a suitable object boundary criterion. We propose an improved search procedure that is more robust against outlier configurations in the boundary target points by requiring subsequent shape changes to be smooth, which is imposed by a smoothness constraint on the displacement of neighbouring target points at each iteration and implemented by a minimal cost path approach. We compare the original ASM search method and our improved search algorithm with a third method that does not rely on iteratively refined target point positions, but instead optimizes a global Bayesian objective function derived from statistical a priori contour shape and image models. Extensive validation of these methods on a database containing more than 400 images of the femur, humerus and calcaneus using the manual expert segmentation as ground truth shows that our minimal cost path method is the most robust. We also evaluate various measures for capturing local image appearance around each boundary point and conclude that the Mahalanobis distance applied to normalized image intensity profiles extracted normal to the shape is the most suitable criterion among the tested ones for guiding the ASM optimization. PMID:11836134

  14. Predicting Pathological Features at Radical Prostatectomy in Patients with Prostate Cancer Eligible for Active Surveillance by Multiparametric Magnetic Resonance Imaging

    PubMed Central

    de Cobelli, Ottavio; Terracciano, Daniela; Tagliabue, Elena; Raimondi, Sara; Bottero, Danilo; Cioffi, Antonio; Jereczek-Fossa, Barbara; Petralia, Giuseppe; Cordima, Giovanni; Almeida, Gilberto Laurino; Lucarelli, Giuseppe; Buonerba, Carlo; Matei, Deliu Victor; Renne, Giuseppe; Di Lorenzo, Giuseppe; Ferro, Matteo

    2015-01-01

    Purpose The aim of this study was to investigate the prognostic performance of multiparametric magnetic resonance imaging (mpMRI) and Prostate Imaging Reporting and Data System (PIRADS) score in predicting pathologic features in a cohort of patients eligible for active surveillance who underwent radical prostatectomy. Methods A total of 223 patients who fulfilled the criteria for “Prostate Cancer Research International: Active Surveillance”, were included. Mp–1.5 Tesla MRI examination staging with endorectal coil was performed at least 6–8 weeks after TRUS-guided biopsy. In all patients, the likelihood of the presence of cancer was assigned using PIRADS score between 1 and 5. Outcomes of interest were: Gleason score upgrading, extra capsular extension (ECE), unfavorable prognosis (occurrence of both upgrading and ECE), large tumor volume (≥0.5ml), and seminal vesicle invasion (SVI). Receiver Operating Characteristic (ROC) curves and Decision Curve Analyses (DCA) were performed for models with and without inclusion of PIRADS score. Results Multivariate analysis demonstrated the association of PIRADS score with upgrading (P<0.0001), ECE (P<0.0001), unfavorable prognosis (P<0.0001), and large tumor volume (P = 0.002). ROC curves and DCA showed that models including PIRADS score resulted in greater net benefit for almost all the outcomes of interest, with the only exception of SVI. Conclusions mpMRI and PIRADS scoring are feasible tools in clinical setting and could be used as decision-support systems for a more accurate selection of patients eligible for AS. PMID:26444548

  15. Use of quantitative shape-activity relationships to model the photoinduced toxicity of polycyclic aromatic hydrocarbons: Electron density shape features accurately predict toxicity

    SciTech Connect

    Mezey, P.G.; Zimpel, Z.; Warburton, P.; Walker, P.D.; Irvine, D.G.; Huang, X.D.; Dixon, D.G.; Greenberg, B.M.

    1998-07-01

    The quantitative shape-activity relationship (QShAR) methodology, based on accurate three-dimensional electron densities and detailed shape analysis methods, has been applied to a Lemna gibba photoinduced toxicity data set of 16 polycyclic aromatic hydrocarbon (PAH) molecules. In the first phase of the studies, a shape fragment QShAR database of PAHs was developed. The results provide a very good match to toxicity based on a combination of the local shape features of single rings in comparison to the central ring of anthracene and a more global shape feature involving larger molecular fragments. The local shape feature appears as a descriptor of the susceptibility of PAHs to photomodification and the global shape feature is probably related to photosensitization activity.

  16. Safety evaluation and risk assessment of the herbicide Roundup and its active ingredient, glyphosate, for humans.

    PubMed

    Williams, G M; Kroes, R; Munro, I C

    2000-04-01

    Reviews on the safety of glyphosate and Roundup herbicide that have been conducted by several regulatory agencies and scientific institutions worldwide have concluded that there is no indication of any human health concern. Nevertheless, questions regarding their safety are periodically raised. This review was undertaken to produce a current and comprehensive safety evaluation and risk assessment for humans. It includes assessments of glyphosate, its major breakdown product [aminomethylphosphonic acid (AMPA)], its Roundup formulations, and the predominant surfactant [polyethoxylated tallow amine (POEA)] used in Roundup formulations worldwide. The studies evaluated in this review included those performed for regulatory purposes as well as published research reports. The oral absorption of glyphosate and AMPA is low, and both materials are eliminated essentially unmetabolized. Dermal penetration studies with Roundup showed very low absorption. Experimental evidence has shown that neither glyphosate nor AMPA bioaccumulates in any animal tissue. No significant toxicity occurred in acute, subchronic, and chronic studies. Direct ocular exposure to the concentrated Roundup formulation can result in transient irritation, while normal spray dilutions cause, at most, only minimal effects. The genotoxicity data for glyphosate and Roundup were assessed using a weight-of-evidence approach and standard evaluation criteria. There was no convincing evidence for direct DNA damage in vitro or in vivo, and it was concluded that Roundup and its components do not pose a risk for the production of heritable/somatic mutations in humans. Multiple lifetime feeding studies have failed to demonstrate any tumorigenic potential for glyphosate. Accordingly, it was concluded that glyphosate is noncarcinogenic. Glyphosate, AMPA, and POEA were not teratogenic or developmentally toxic. There were no effects on fertility or reproductive parameters in two multigeneration reproduction studies with

  17. Third annual report of health activities under the Federal Mine Safety and Health Act of 1977

    SciTech Connect

    Not Available

    1981-02-01

    The federal mine health program in 1980 is discussed. Health hazard evaluations were completed for exposure to crystalline silica (7631869), oil shale, perchloroethylene (127184), and heat stress. A report from the coal workers health surveillance program is presented. Mine health research projects are reported on diesel exhausts, vibration, manual materials handling, coal workers pneumoconiosis, new miner studies, stomach cancer, metal toxicity on lung cells, lipid peroxidation, and diesel and metal particulates effects on interferon synthesis. Criteria documentation, standards development, and related research on radon daughters, heat stress, asbestos, silica, welding fumes, mine sanitation, and emergency medical services are reported. Respirators, personal dust samplers, and other safety equipment testing and certification are reported and mine health education programs are presented. This report represents an effort by NIOSH to identify, evaluate, and control work related disease among miners.

  18. Active medical implants and occupational safety--measurement and numerical calculation of interference voltage.

    PubMed

    Gustrau, F; Bahr, A; Goltz, S; Eggert, S

    2002-01-01

    Low frequency electric and magnetic fields may interfere with implanted cardiac pacemakers causing a life-threatening malfunction of the device. In order to assess the safety of workers in the vicinity of industrial electrical devices the interference voltage at the input port of a pacemaker is an important measure. In order to investigate the coupling of fields emanating from electrical devices a numerical method for the calculation of interference voltages is presented and applied to the investigation of homogeneous electric and magnetic fields in the frequency range from 50 Hz to 1 MHz. Implantation of the pacemaker in the right pectoral, left pectoral and abdominal area using a realistic model of the human body as well as different grounding conditions are considered. The numerical method is successfully validated by measurements and shows good agreement with results in the literature.

  19. Analyses comparing the antimicrobial activity and safety of current antiseptic agents: a review.

    PubMed

    Hibbard, John S

    2005-01-01

    This article reviews the results and conclusions from four pivotal and two comparative clinical trials. The six randomized, controlled, single-blinded, parallel-group clinical trials were conducted to determine which antiseptic is best for use as a patient preoperative skin preparation. The objective of these studies was to compare the immediate, persistent (residual), and cumulative antimicrobial efficacy and safety of 2% chlorhexidine gluconate (CHG) combined with 70% isopropyl alcohol (IPA) (ChloraPrep); another combination CHG and IPA antiseptic (CHG+IPA) and 2% aqueous CHG alone; 4% CHG (Hibiclens) alone; 70% isopropyl alcohol (IPA) alone; and an iodine-containing solution, 10% povidone-iodine (Betadine) alone as preoperative skin topical antiseptics for potential prevention of nosocomial infections.

  20. Ethnic minority children’s active commuting to school and association with physical activity and pedestrian safety behaviors

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Children's active commuting to school, i.e. walking or cycling to school, was associated with greater moderate-to-vigorous physical activity, although studies among ethnic minorities are sparse. Among a low-income, ethnic minority sample of fourth grade students from eight public schools, we examine...

  1. Is wetter better? An evaluation of over-the-counter personal lubricants for safety and anti-HIV-1 activity.

    PubMed

    Dezzutti, Charlene S; Brown, Elizabeth R; Moncla, Bernard; Russo, Julie; Cost, Marilyn; Wang, Lin; Uranker, Kevin; Kunjara Na Ayudhya, Ratiya P; Pryke, Kara; Pickett, Jim; Leblanc, Marc-André; Rohan, Lisa C

    2012-01-01

    Because lubricants may decrease trauma during coitus, it is hypothesized that they could aid in the prevention of HIV acquisition. Therefore, safety and anti-HIV-1 activity of over-the-counter (OTC) aqueous- (n = 10), lipid- (n = 2), and silicone-based (n = 2) products were tested. The rheological properties of the lipid-based lubricants precluded testing with the exception of explant safety testing. Six aqueous-based gels were hyperosmolar, two were nearly iso-osmolar, and two were hypo-osmolar. Evaluation of the panel of products showed Gynol II (a spermicidal gel containing 2% nonoxynol-9), KY Jelly, and Replens were toxic to Lactobacillus. Two nearly iso-osmolar aqueous- and both silicone-based gels were not toxic toward epithelial cell lines or ectocervical or colorectal explant tissues. Hyperosmolar lubricants demonstrated reduction of tissue viability and epithelial fracture/sloughing while the nearly iso-osmolar and silicon-based lubricants showed no significant changes in tissue viability or epithelial modifications. While most of the lubricants had no measurable anti-HIV-1 activity, three lubricants which retained cell viability did demonstrate modest anti-HIV-1 activity in vitro. To determine if this would result in protection of mucosal tissue or conversely determine if the epithelial damage associated with the hyperosmolar lubricants increased HIV-1 infection ex vivo, ectocervical tissue was exposed to selected lubricants and then challenged with HIV-1. None of the lubricants that had a moderate to high therapeutic index protected the mucosal tissue. These results show hyperosmolar lubricant gels were associated with cellular toxicity and epithelial damage while showing no anti-viral activity. The two iso-osmolar lubricants, Good Clean Love and PRÉ, and both silicone-based lubricants, Female Condom 2 lubricant and Wet Platinum, were the safest in our testing algorithm.

  2. Is wetter better? An evaluation of over-the-counter personal lubricants for safety and anti-HIV-1 activity.

    PubMed

    Dezzutti, Charlene S; Brown, Elizabeth R; Moncla, Bernard; Russo, Julie; Cost, Marilyn; Wang, Lin; Uranker, Kevin; Kunjara Na Ayudhya, Ratiya P; Pryke, Kara; Pickett, Jim; Leblanc, Marc-André; Rohan, Lisa C

    2012-01-01

    Because lubricants may decrease trauma during coitus, it is hypothesized that they could aid in the prevention of HIV acquisition. Therefore, safety and anti-HIV-1 activity of over-the-counter (OTC) aqueous- (n = 10), lipid- (n = 2), and silicone-based (n = 2) products were tested. The rheological properties of the lipid-based lubricants precluded testing with the exception of explant safety testing. Six aqueous-based gels were hyperosmolar, two were nearly iso-osmolar, and two were hypo-osmolar. Evaluation of the panel of products showed Gynol II (a spermicidal gel containing 2% nonoxynol-9), KY Jelly, and Replens were toxic to Lactobacillus. Two nearly iso-osmolar aqueous- and both silicone-based gels were not toxic toward epithelial cell lines or ectocervical or colorectal explant tissues. Hyperosmolar lubricants demonstrated reduction of tissue viability and epithelial fracture/sloughing while the nearly iso-osmolar and silicon-based lubricants showed no significant changes in tissue viability or epithelial modifications. While most of the lubricants had no measurable anti-HIV-1 activity, three lubricants which retained cell viability did demonstrate modest anti-HIV-1 activity in vitro. To determine if this would result in protection of mucosal tissue or conversely determine if the epithelial damage associated with the hyperosmolar lubricants increased HIV-1 infection ex vivo, ectocervical tissue was exposed to selected lubricants and then challenged with HIV-1. None of the lubricants that had a moderate to high therapeutic index protected the mucosal tissue. These results show hyperosmolar lubricant gels were associated with cellular toxicity and epithelial damage while showing no anti-viral activity. The two iso-osmolar lubricants, Good Clean Love and PRÉ, and both silicone-based lubricants, Female Condom 2 lubricant and Wet Platinum, were the safest in our testing algorithm. PMID:23144863

  3. Is Wetter Better? An Evaluation of Over-the-Counter Personal Lubricants for Safety and Anti-HIV-1 Activity

    PubMed Central

    Dezzutti, Charlene S.; Brown, Elizabeth R.; Moncla, Bernard; Russo, Julie; Cost, Marilyn; Wang, Lin; Uranker, Kevin; Kunjara Na Ayudhya, Ratiya P.; Pryke, Kara; Pickett, Jim; LeBlanc, Marc-André; Rohan, Lisa C.

    2012-01-01

    Because lubricants may decrease trauma during coitus, it is hypothesized that they could aid in the prevention of HIV acquisition. Therefore, safety and anti-HIV-1 activity of over-the-counter (OTC) aqueous- (n = 10), lipid- (n = 2), and silicone-based (n = 2) products were tested. The rheological properties of the lipid-based lubricants precluded testing with the exception of explant safety testing. Six aqueous-based gels were hyperosmolar, two were nearly iso-osmolar, and two were hypo-osmolar. Evaluation of the panel of products showed Gynol II (a spermicidal gel containing 2% nonoxynol-9), KY Jelly, and Replens were toxic to Lactobacillus. Two nearly iso-osmolar aqueous- and both silicone-based gels were not toxic toward epithelial cell lines or ectocervical or colorectal explant tissues. Hyperosmolar lubricants demonstrated reduction of tissue viability and epithelial fracture/sloughing while the nearly iso-osmolar and silicon-based lubricants showed no significant changes in tissue viability or epithelial modifications. While most of the lubricants had no measurable anti-HIV-1 activity, three lubricants which retained cell viability did demonstrate modest anti-HIV-1 activity in vitro. To determine if this would result in protection of mucosal tissue or conversely determine if the epithelial damage associated with the hyperosmolar lubricants increased HIV-1 infection ex vivo, ectocervical tissue was exposed to selected lubricants and then challenged with HIV-1. None of the lubricants that had a moderate to high therapeutic index protected the mucosal tissue. These results show hyperosmolar lubricant gels were associated with cellular toxicity and epithelial damage while showing no anti-viral activity. The two iso-osmolar lubricants, Good Clean Love and PRÉ, and both silicone-based lubricants, Female Condom 2 lubricant and Wet Platinum, were the safest in our testing algorithm. PMID:23144863

  4. Bathroom safety - children

    MedlinePlus

    ... this page: //medlineplus.gov/ency/patientinstructions/000154.htm Bathroom safety - children To use the sharing features on ... please enable JavaScript. To prevent accidents in the bathroom, never leave your child alone in the bathroom. ...

  5. How to evaluate the risks of work equipment and installations for health and safety? Research and activities of the German Committee for Plant Safety and consequences for regulation.

    PubMed

    Pieper, R

    2012-01-01

    Work equipment and installations with a high risk for health and safety of employees should be paid a special attention. The German Product Safety Act, which is aimed to manufacturers or distributors in order to protect consumers, maintains a conclusive catalogue of these so-called "installations in need of monitoring" fixing the work equipment and installations for which such special inspections can be demanded. This catalogue has remained unchanged for decades and has been transformed nearly unmodified into the Plant Safety Ordinance. Currently, there is a discussion about this catalogue in Germany. A major point of concern is the definition and the significance of "especially" dangerous work equipment and installations. Two recent research projects are dealing with the problem how to define "especially".

  6. CONVEYOR SYSTEM SAFETY ANALYSIS

    SciTech Connect

    M. Salem

    1995-06-23

    The purpose and objective of this analysis is to systematically identify and evaluate hazards related to the Yucca Mountain Project Exploratory Studies Facility (ESF) surface and subsurface conveyor system (for a list of conveyor subsystems see section 3). This process is an integral part of the systems engineering process; whereby safety is considered during planning, design, testing, and construction. A largely qualitative approach was used since a radiological System Safety Analysis is not required. The risk assessment in this analysis characterizes the accident scenarios associated with the conveyor structures/systems/components in terms of relative risk and includes recommendations for mitigating all identified risks. The priority for recommending and implementing mitigation control features is: (1) Incorporate measures to reduce risks and hazards into the structure/system/component (S/S/C) design, (2) add safety devices and capabilities to the designs that reduce risk, (3) provide devices that detect and warn personnel of hazardous conditions, and (4) develop procedures and conduct training to increase worker awareness of potential hazards, on methods to reduce exposure to hazards, and on the actions required to avoid accidents or correct hazardous conditions. The scope of this analysis is limited to the hazards related to the design of conveyor structures/systems/components (S/S/Cs) that occur during normal operation. Hazards occurring during assembly, test and maintenance or ''off normal'' operations have not been included in this analysis. Construction related work activities are specifically excluded per DOE Order 5481.1B section 4. c.

  7. TWRS safety program plan

    SciTech Connect

    Calderon, L.M., Westinghouse Hanford

    1996-08-01

    Management of Nuclear Safety, Industrial Safety, Industrial Hygiene, and Fire Protection programs, functions, and field support resources for Tank Waste Remediation Systems (TWRS) has, until recently, been centralized in TWRS Safety, under the Emergency, Safety, and Quality organization. Industrial hygiene technician services were also provided to support operational needs related to safety basis compliance. Due to WHC decentralization of safety and reengineering efforts in West Tank Farms, staffing and safety responsibilities have been transferred to the facilities. Under the new structure, safety personnel for TWRS are assigned directly to East Tank Farms, West Tank Farms, and a core Safety Group in TWRS Engineering. The Characterization Project Operations (CPO) safety organization will remain in tact as it currently exists. Personnel assigned to East Tank Farms, West Tank Farms, and CPO will perform facility-specific or project-specific duties and provide field implementation of programs. Those assigned to the core group will focus on activities having a TWRS-wide or programmatic focus. Hanford-wide activities will be the responsibility of the Safety Center of Expertise. In order to ensure an effective and consistent safety program for TWRS under the new organization program functions, goals, organizational structure, roles, responsibilities, and path forward must be clearly established. The purpose of the TWRS Safety Program Plan is to define the overall safety program, responsibilities, relationships, and communication linkages for safety personnel under the new structure. In addition, issues associated with reorganization transition are addressed, including training, project ownership, records management, and dissemination of equipment. For the purpose of this document ``TWRS Safety`` refers to all safety professionals and technicians (Industrial Safety, Industrial Hygiene, Fire Protection, and Nuclear Safety) within the TWRS organization, regardless of their

  8. Combined control effects of brake and active suspension control on the global safety of a full-car nonlinear model

    NASA Astrophysics Data System (ADS)

    Tchamna, Rodrigue; Youn, Edward; Youn, Iljoong

    2014-05-01

    This paper focuses on the active safety of a full-vehicle nonlinear model during cornering. At first, a previously developed electronic stability controller (ESC) based on vehicle simplified model is applied to the full-car nonlinear model in order to control the vehicle yaw rate and side-slip angle. The ESC system was shown beneficial not only in tracking the vehicle path as close as possible, but it also helped in reducing the vehicle roll angle and influences ride comfort and road-holding capability; to tackle that issue and also to have better attitude motion, making use of optimal control theory the active suspension control gain is developed from a vehicle linear model and used to compute the active suspension control force of the vehicle nonlinear model. The active suspension control algorithm used in this paper includes the integral action of the suspension deflection in order to make zero the suspension deflection steady state and keep the vehicle chassis flat. Keeping the chassis flat reduces the vehicle load transfer and that is helpful for road holding and yaw rate tracking. The effects of the two controllers when they work together are analysed using various computer simulations with different steering wheel manoeuvres.

  9. Aerosol Gemcitabine: Preclinical Safety and In Vivo Antitumor Activity in Osteosarcoma-Bearing Dogs

    PubMed Central

    Crabbs, Torrie A.; Wilson, Dennis W.; Cannan, Virginia A.; Skorupski, Katherine A.; Gordon, Nancy; Koshkina, Nadya; Kleinerman, Eugenie; Anderson, Peter M.

    2010-01-01

    Abstract Background Osteosarcoma is the most common skeletal malignancy in the dog and in young humans. Although chemotherapy improves survival time, death continues to be attributed to metastases. Aerosol delivery can provide a strategy with which to improve the lung drug delivery while reducing systemic toxicity. The purpose of this study is to assess the safety of a regional aerosol approach to chemotherapy delivery in osteosarcoma-bearing dogs, and second, to evaluate the effect of gemcitabine on Fas expression in the pulmonary metastasis. Methods We examined the systemic and local effects of aerosol gemcitabine on lung and pulmonary metastasis in this relevant large-animal tumor model using serial laboratory and arterial blood gas analysis and histopathology and immunohistochemistry, respectively. Results and Conclusions Six hundred seventy-two 1-h doses of aerosol gemcitabine were delivered. The treatment was well tolerated by these subjects with osteosarcoma (n = 20). Aerosol-treated subjects had metastatic foci that demonstrated extensive, predominately central, intratumoral necrosis. Fas expression was decreased in pulmonary metastases compared to the primary tumor (p = 0.008). After aerosol gemcitabine Fas expression in the metastatic foci was increased compared to lung metastases before treatment (p = 0.0075), and even was higher than the primary tumor (p = 0.025). Increased apoptosis (TUNEL) staining was also detected in aerosol gemcitabine treated metastasis compared to untreated controls (p = 0.028). The results from this pivotal translational study support the concept that aerosol gemcitabine may be useful against pulmonary metastases of osteosarcoma. Additional studies that evaluate the aerosol route of administration of gemcitabine in humans should be safe and are warranted. PMID:19803732

  10. Health and safety economics: limitations of economic appraisal of occupational health services activities in Poland.

    PubMed

    Rydlewska-Liszkowska, Izabela

    2002-01-01

    Methods of economic appraisal developed for evaluating activities in health care system may as well be successfully used for evaluating occupational health service activities. This involves the problem of resources management and cost containment not only at the company level, but also at different managerial and institutional levels. The decision makers have to know what resources are spent on occupational health, what is the effectiveness and efficiency of investing in employees health. The key issue of good understanding of the theory and practice of economic appraisal is a precise definition of costs, effectiveness and benefits. Another important area is the identification of information sources and barriers of economic appraisal. The results of the project carried out by the Nofer Institute of Occupational Medicine have provided evidence that defining costs, effectiveness and benefits of preventive activities need to be developed. It becomes even more clear after an analysis of existing limitations of economic appraisal in Polish enterprises.

  11. Migration studies to assess the safety in use of a new antioxidant active packaging.

    PubMed

    Tovar, Laura; Salafranca, Jesús; Sánchez, Cristina; Nerín, Cristina

    2005-06-29

    Both specific and overall migration tests have been applied to new experimental food packaging-active plastic films with antioxidant properties, including in its composition a natural rosemary extract. Determination of volatile and semivolatile compounds migrating from plastic to the four established simulants showed that both specific and overall migration was very low. The results obtained gave values 20 times lower than the established limits in the worst case. So, from the point of view of health risk, the new active packaging can be considered as safe. Analytical procedure used provided the necessary information about the migration behavior, with good analytical characteristics and detection limits in the sub mug kg(-1) range. Besides, no significant difference was found between laboratory and factory-made samples, which is an important issue for industrial production, the next step in the development of the new antioxidant active film. PMID:15969507

  12. Design of a reusable kinetic energy absorber for an astronaut safety tether to be used during extravehicular activities on the Space Station

    NASA Technical Reports Server (NTRS)

    Borthwick, Dawn E.; Cronch, Daniel F.; Nixon, Glen R.

    1991-01-01

    The goal of this project is to design a reusable safety device for a waist tether which will absorb the kinetic energy of an astronaut drifting away from the Space Station. The safety device must limit the tension of the tether line in order to prevent damage to the astronaut's space suit or to the structure of the spacecraft. The tether currently used on shuttle missions must be replaced after the safety feature has been developed. A reusable tether for the Space Station would eliminate the need for replacement tethers, conserving space and mass. This report presents background information, scope and limitations, methods of research and development, alternative designs, a final design solution and its evaluation, and recommendations for further work.

  13. [The efficacy and safety of activated vitamin D for CKD-MBD].

    PubMed

    Hayakawa, Nobuki; Suzuki, Atsushi

    2016-09-01

    Metabolic bone disorders that are represented by secondary hyperparathyroidism occur with the progression of chronic kidney disease(CKD). The administration of activated vitamin D is expected to improve high-turnover bone disorders and is widely used for the management of bone mineral diseases in patients with CKD and end-stage renal disease. CKD is an underlying disease of secondary osteoporosis and coexists with primary osteoporosis at a high rate. With regard to osteoporosis patients with renal insufficiency, the administration of activated vitamin D is also thought to reduce the fracture incidence by both increasing bone mass and reducing falls. PMID:27561349

  14. Safety and immunogenicity of recombinant poxvirus HIV-1 vaccines in young adults on highly active antiretroviral therapy

    PubMed Central

    Greenough, Thomas C.; Cunningham, Coleen K.; Muresan, Petronella; McManus, Margaret; Persaud, Deborah; Fenton, Terry; Barker, Piers; Gaur, Aditya; Panicali, Dennis; Sullivan, John L.; Luzuriaga, Katherine

    2008-01-01

    A trial to evaluate the safety and immunogenicity of recombinant modified vaccinia Ankara (MVA) and fowlpox (FP) vectors expressing multiple HIV-1 proteins was conducted in twenty HIV-1 infected youth with suppressed viral replication on HAART. The MVA and FP-based multigene HIV-1 vaccines were safe and well tolerated. Increased frequencies of HIV-1 specific CD4+ proliferative responses and cytokine secreting cells were detected following immunization. Increased frequencies and breadth of HIV-1 specific CD8+ T cell responses were also detected. Plasma HIV-1–specific antibody levels and neutralizing activity were unchanged following vaccination. Poxvirus based vaccines may merit further study in therapeutic vaccine protocols. PMID:18940219

  15. Sample Heat, Activity, Reactivity, and Dose Analysis for Safety Analysis of Irradiations in a Research Reactor.

    1987-12-01

    SHARDA is a program for assessing sample heating rates, activities produced and reactivity load caused while irradiating a small sample in a well thermalized research reactor like CIRUS. It estimates the sample cooling or lead shielding requirements to limit the gamma-ray dose rates due to the irradiated sample within permissible levels.

  16. 76 FR 72997 - Railroad Safety Advisory Committee (RSAC); Working Group Activity Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... for additional language. The NPRM was published on January 12, 2011 (76 FR 2200), and the final rule... announcement of working group activities and status reports of December 7, 2010 (75 FR 76070). The 44th full..., 2006 (71 FR 50275), and was open for comment until October 23, 2006. The working group agreed...

  17. 75 FR 51525 - Railroad Safety Advisory Committee (RSAC); Working Group Activity Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-20

    ... announcement of working group activities and status reports of January 29, 2010 (75 FR 4904). The 41st full... October 11, 2005. The Notice of Proposed Rulemaking (NPRM) was published on August 24, 2006 (71 FR 50275... published on February 1, 2008 (73 FR 6370). The Task Force met on October 17-18, 2007, and reached...

  18. 77 FR 24257 - Railroad Safety Advisory Committee (RSAC); Working Group Activity Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-23

    ... announcement of working group activities and status reports of November 28, 2011 (76 FR 72997). The 45th full... Rulemaking (NPRM) was published on August 24, 2006 (71 FR 50275), and was open for comment until October 23... emergency communication, emergency egress, and rescue access, was published on February 1, 2008 (73 FR...

  19. 77 FR 58608 - Railroad Safety Advisory Committee (RSAC); Working Group Activity Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-21

    ... announcement of working group activities and status reports of April 23, 2012 (77 FR 24257). The 46th full RSAC... published on August 24, 2006 (71 FR 50275), and was open for comment until October 23, 2006. The working... communication, emergency egress, and rescue access, was published on February 1, 2008 (73 FR 6370). The...

  20. 75 FR 76070 - Railroad Safety Advisory Committee (RSAC); Working Group Activity Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-07

    ... announcement of working group activities and status reports of August 20, 2010 (75 FR 51525). The 42nd full... Rulemaking (NPRM) was published on August 24, 2006 (71 FR 50275), and was open for comment until October 23... emergency communication, emergency egress, and rescue access, was published on February 1, 2008 (73 FR...

  1. Multibeam Mapping of Active Slope Instability Features: Examples from the Fraser River and Squamish River Deltas, British Columbia, Canada

    NASA Astrophysics Data System (ADS)

    Hill, P. R.

    2004-12-01

    Multibeam mapping of the coastal waters of British Columbia has immensly improved our ability to identify and assess submarine landslide and tsunami hazard. This paper will present analysis of high-resolution images of slope instability features from two delta slopes where recent slope failure can be documented through repetitive multibeam mapping and/or comparison with previous single-beam hydrographic soundings. Numerous mass movement features characterize the slope of the Fraser River delta, all the recent features being located at the mouths of distributary channels. Engineering works have maintained the main channel in a fixed position since the 1930's, contributing to over-steepening of the slope and development of a network of submarine channels. Repetitive multibeam mapping shows that recent slope failures have occurred in numerous locations around the main channel lobe, some at the head of a large submarine channel system and others as isolated small failures that form the headwalls of small submarine channels. The scalloped morphology and association with channels, together with volume estimates derived from repetitive multibeam mapping, indicate that these features result from shallow, small volume liquefaction failures. Smaller scale, shallow slides are present on the very shallow water slope area adjacent to the channels, raising the possibility of groundwater seepage as an influence on slope stability. The slide masses from these failures are rapidly transformed into gravity flows that carve the submarine channels. Slides and channels of a similar scale are found at the mouth of a secondary distributary channel and an abandoned distributary channel. The multibeam imagery allows discrimination between recent slide features and relict features, the latter showing infilling or reworking by bottom currents. An area of undulatory seafloor, located on the flank of the main distributary channel lobe, has been cited as a possible creep displacement feature

  2. Slope Stability: Factor of Safety along the Seismically Active Continental Slope Offshore Sumatra

    NASA Astrophysics Data System (ADS)

    Patton, J. R.; Goldfinger, C.; Djadjadihardja, Y.; None, U.

    2013-12-01

    Recent papers have documented the probability that turbidites deposited along and downslope of subduction zone accretionary prisms are likely the result of strong ground shaking from great earthquakes. Given the damaging nature of these earthquakes, along with the casualties from the associated tsunamis, the spatial and temporal patterns of these earthquakes can only be evaluated with paleoseismologic coring and seismic reflection methods. We evaluate slope stability for seafloor topography along the Sunda subduction offshore Sumatra, Indonesia. We use sediment material properties, from local (Sumatra) and analogous sites, to constrain our estimates of static slope stability Factor of Safety (FOS) analyses. We then use ground motion prediction equations (GMPE's) to estimate ground motion intensity (Arias Intensity, AI) and acceleration (Peak Ground Acceleration, PGA), as possibly generated by fault rupture, to constrain seismic loads for pseudostatic slope stability FOS analyses. The ground motions taper rapidly with distance from the fault plane, consistent with ground motion - fault distance relations measured during the 2011 Tohoku-Oki subduction zone earthquake. Our FOS analyses include a Morgenstern method of slices probabilistic analysis for 2-D profiles along with Critical Acceleration (Ac) and Newmark Displacement (Dn) analysis of multibeam bathymetry of the seafloor. In addition, we also use estimates of ground motion modeled with a 2004 Sumatra-Andaman subduction zone (SASZ) earthquake fault slip model, to also compare with our static FOS analyses of seafloor topography. All slope and trench sites are statically stable (FOS < 1) and sensitive to ground motions generated by earthquakes of magnitude greater than 7. We conclude that for earthquakes of magnitude 6 to 9, PGA of 0.4-0.6 to 1.4-2.5 g would be expected, respectively, from existing GMPE's. However, saturation of accelerations in the accretionary wedge may limit actual accelerations to less than 1

  3. Safety and Efficacy of Intrapleural Tissue Plasminogen Activator and DNase during Extended Use in Complicated Pleural Space Infections

    PubMed Central

    McClune, Jason R.; Wilshire, Candice L.; Gorden, Jed A.; Louie, Brian E.; Farviar, Alexander S.; Stefanski, Michael J.; Vallieres, Eric; Aye, Ralph W.

    2016-01-01

    The use of intrapleural therapy with tissue plasminogen activator and DNase improves outcomes in patients with complicated pleural space infections. However, little data exists for the use of combination intrapleural therapy after the initial dosing period of six doses. We sought to describe the safety profile and outcomes of intrapleural therapy beyond this standard dosing. A retrospective review of patients receiving intrapleural therapy with tissue plasminogen activator and DNase was performed at two institutions. We identified 101 patients from January 2013 to August 2015 receiving intrapleural therapy for complicated pleural space infection. The extended use of intrapleural tissue plasminogen activator and DNase therapy beyond six doses was utilized in 20% (20/101) of patients. The mean number of doses in those undergoing extended dosing was 9.8 (range of 7–16). Within the population studied there appears to be no statistically significant increased risk of complications, need for surgical referral, or outcome differences when comparing those receiving standard or extended dosing intrapleural therapy. Future prospective study of intrapleural therapy as an alternative option for patients who fail initial pleural drainage and are unable to tolerate/accept a surgical intervention appears a potential area of study. PMID:27445574

  4. Safety and Efficacy of Intrapleural Tissue Plasminogen Activator and DNase during Extended Use in Complicated Pleural Space Infections.

    PubMed

    McClune, Jason R; Wilshire, Candice L; Gorden, Jed A; Louie, Brian E; Farviar, Alexander S; Stefanski, Michael J; Vallieres, Eric; Aye, Ralph W; Gilbert, Christopher R

    2016-01-01

    The use of intrapleural therapy with tissue plasminogen activator and DNase improves outcomes in patients with complicated pleural space infections. However, little data exists for the use of combination intrapleural therapy after the initial dosing period of six doses. We sought to describe the safety profile and outcomes of intrapleural therapy beyond this standard dosing. A retrospective review of patients receiving intrapleural therapy with tissue plasminogen activator and DNase was performed at two institutions. We identified 101 patients from January 2013 to August 2015 receiving intrapleural therapy for complicated pleural space infection. The extended use of intrapleural tissue plasminogen activator and DNase therapy beyond six doses was utilized in 20% (20/101) of patients. The mean number of doses in those undergoing extended dosing was 9.8 (range of 7-16). Within the population studied there appears to be no statistically significant increased risk of complications, need for surgical referral, or outcome differences when comparing those receiving standard or extended dosing intrapleural therapy. Future prospective study of intrapleural therapy as an alternative option for patients who fail initial pleural drainage and are unable to tolerate/accept a surgical intervention appears a potential area of study.

  5. Safety and Efficacy of Intrapleural Tissue Plasminogen Activator and DNase during Extended Use in Complicated Pleural Space Infections.

    PubMed

    McClune, Jason R; Wilshire, Candice L; Gorden, Jed A; Louie, Brian E; Farviar, Alexander S; Stefanski, Michael J; Vallieres, Eric; Aye, Ralph W; Gilbert, Christopher R

    2016-01-01

    The use of intrapleural therapy with tissue plasminogen activator and DNase improves outcomes in patients with complicated pleural space infections. However, little data exists for the use of combination intrapleural therapy after the initial dosing period of six doses. We sought to describe the safety profile and outcomes of intrapleural therapy beyond this standard dosing. A retrospective review of patients receiving intrapleural therapy with tissue plasminogen activator and DNase was performed at two institutions. We identified 101 patients from January 2013 to August 2015 receiving intrapleural therapy for complicated pleural space infection. The extended use of intrapleural tissue plasminogen activator and DNase therapy beyond six doses was utilized in 20% (20/101) of patients. The mean number of doses in those undergoing extended dosing was 9.8 (range of 7-16). Within the population studied there appears to be no statistically significant increased risk of complications, need for surgical referral, or outcome differences when comparing those receiving standard or extended dosing intrapleural therapy. Future prospective study of intrapleural therapy as an alternative option for patients who fail initial pleural drainage and are unable to tolerate/accept a surgical intervention appears a potential area of study. PMID:27445574

  6. New Cloud Activity on Uranus in 2004: First Detection of a Southern Feature at 2.2 microns

    SciTech Connect

    Hammel, H B; de Pater, I; Gibbard, S; Lockwood, G; Rages, K

    2005-02-02

    On 4 July 2004 UT, we detected one of Uranus' southern hemispheric features at K' (2.2 {micro}m); this is the first such detection in half a decade of adaptive optics imaging of Uranus at the Keck 10-m telescope. When we observed again on 8 July UT the core had faded, and by 9 July UT it was not seen at K' and barely detectable at H. The detection and subsequent disappearance of the feature indicates rapid dynamical processes in the localized vertical aerosol structure.

  7. Evaluation of Aryoseven Safety (Recombinant Activated Factor VII) in Patients with Bleeding Disorders (An Observational Post-Marketing Surveillance Study)

    PubMed Central

    Toogeh, Gholamreza; Abolghasemi, Hassan; Eshghi, Peyman; Managhchi, Mohammadreza; Shaverdi-niasari, Mohammadreza; Karimi, Katayoon; Roostaei, Samin; Emran, Neda; Abdollahi, Alireza

    2016-01-01

    Background: Recombinant activated factor VII induces hemostasis in patients with coagulopathy disorders. AryoSeven™ as a safe Iranian Recombinant activated factor VII has been available on our market. This study was performed to establish the safety of AryoSeven on patients with coagulopathy disorder. Methods: This single-center, descriptive, cross sectional study was carried out in Thrombus and Homeostasis Research Center ValiAsr Hospital during 2013-2014. Fifty one patients with bleeding disorders who received at least one dose of Aryoseven were enrolled. Patients’ demographic data and adverse effect of drug and reaction related to Aryoseven or previous usage of Recombinant activated FVII were recorded in questionnaires. Finally data were analyzed to compare side effects of Aryoseven and other Recombinant activated FVII brands. Results: Aryoseven was prescribed for 51 Patients. Of all participants with mean age 57.18+21.38 yr, 31 cases were male and 26 subjects had past history of recombinant activated FVII usage. Glanzman was the most frequent disorder followed by congenital FVII deficiency, hemophilia with inhibitors, factor 5 deficiency, acquired hemophilia, hemophilia A with inhibitor, and hemophilia A or B with inhibitor. The majority of bleeding episodes had occurred in joints. Three patients (5.9%) complained about adverse effects of Aryoseven vs. 11.5 % about adverse effects of other brands. However this difference was not significant, statistically. Conclusion: Based on monitor patients closely for any adverse events, we concluded that Aryoseven administration under careful weighing of benefit versus potential harm may comparable with other counterpart drugs. PMID:27799968

  8. The circadian rest-activity rhythm, a potential safety pharmacology endpoint of cancer chemotherapy.

    PubMed

    Ortiz-Tudela, Elisabet; Iurisci, Ida; Beau, Jacques; Karaboue, Abdoulaye; Moreau, Thierry; Rol, Maria Angeles; Madrid, Juan Antonio; Lévi, Francis; Innominato, Pasquale F

    2014-06-01

    The robustness of the circadian timing system (CTS) was correlated to quality of life and predicted for improved survival in cancer patients. However, chemotherapy disrupted the CTS according to dose and circadian timing in mice. A continuous and repeated measures longitudinal design was implemented here to characterize CTS dynamics in patients receiving a fixed circadian-based chemotherapy protocol. The rest-activity rhythm of 49 patients with advanced cancer was monitored using a wrist actigraph for 13 days split into four consecutive spans of 3-4 days each, i.e., before, during, right after and late after a fixed chronotherapy course. The relative amount of activity in bed vs. out of bed (Iactivity rhythm parameters being worsened in the whole group of patients (p < 0.05). Mean parameter values subsequently recovered to near baseline values. The occurrence of circadian disruption on chemotherapy was associated with a higher risk of clinically relevant fatigue (p = 0.028) or body weight loss (p = 0.05). Four CTS dynamic patterns characterized treatment response including no change (9.5% of the patients); improvement (14.3%); alteration and complete recovery (31%) or sustained deterioration (45%), possibly due to inadequate chronotherapy dosing and/or timing. Improved clinical tolerability could result from the minimization of circadian disruption through the personalization of chronotherapy delivery.

  9. Safety and Efficacy of Activated Transfected Killer Cells for Neutropenic Fungal Infections

    PubMed Central

    Lin, Lin; Ibrahim, Ashraf S.; Baquir, Beverlie; Fu, Yue; Applebaum, David; Schwartz, Julie; Wang, Amy; Avanesian, Valentina; Spellberg, Brad

    2010-01-01

    Background Invasive fungal infections cause considerable morbidity and mortality in neutropenic patients. White blood cell transfusions are a promising treatment for such infections, but technical barriers have prevented their widespread use. Methods To recapitulate white blood cell transfusions, we are developing a cell-based immunotherapy using a phagocytic cell line, HL-60. We sought to stably transfect HL-60 cells with a suicide trap (herpes simplex virus thymidine kinase), to enable purging of the cells when desired, and a bioluminescence marker, to track the cells in vivo in mice. Results Transfection was stable despite 20 months of continuous culture or storage in liquid nitrogen. Activation of these transfected cells with retinoic acid and dimethyl sulfamethoxazole enhanced their microbicidal effects. Activated transfected killer (ATAK) cells were completely eliminated after exposure to ganciclovir, confirming function of the suicide trap. ATAK cells improved the survival of neutropenic mice with lethal disseminated candidiasis and inhalational aspergillosis. Bioluminescence and histopathologic analysis confirmed that the cells were purged from surviving mice after ganciclovir treatment. Comprehensive necropsy, histopathology, and metabolomic analysis revealed no toxicity of the cells. Conclusions These results lay the groundwork for continued translational development of this promising, novel technology for the treatment of refractory infections in neutropenic hosts. PMID:20397927

  10. Safety and antidiarrheal activity of Priva adhaerens aqueous leaf extract in a murine model

    PubMed Central

    Nansunga, Miriam; Barasa, Ambrose; Abimana, Justus; Alele, Paul E.; Kasolo, Josephine

    2014-01-01

    Ethnopharmacological relevance Priva adhaerens (Forssk.) Chiov., a wildly growing plant, is reported in central Uganda to be an effective traditional remedy for diarrhea. The objective of this study was to provide a scientific basis for the ethnopharmacological utility of this plant whose aqueous leaf and shoot extract was evaluated for acute toxicity and antidiarrheal activity using a murine model. Materials and methods Acute toxicity of the aqueous leaf and shoot extract was assessed after determining the major phytochemicals present in the extract. The aqueous leaf and shoot extract was assayed against castor oil-induced diarrhea, transit time, and enteropooling, in comparison to loperamide, a standard drug. Results The oral LD50 value obtained for Priva adhaerens aqueous extract was greater than 5000 mg/kg in rats; the aqueous leaf and shoot extract possessed several important phytochemicals. Furthermore, the aqueous extract significantly, and dose-dependently, reduced frequency of stooling in castor oil-induced diarrhea, intestinal motility, and castor oil-induced enteropooling in rats. Conclusion This murine model shows that it is relatively safe to orally use the aqueous leaf and shoot extract of Priva adhaerens . The aqueous extract contains phytochemicals that are active for the treatment of diarrhea in a rat model. PMID:25304198

  11. Safety Audit/Inspection Manual.

    ERIC Educational Resources Information Center

    American Chemical Society, Washington, DC.

    This guide provides guidelines and procedures for safety audits and inspections in work environments. Contents include: (1) Administrative Concepts, (2) Physical Concepts, (3) Protecting Your Audits, (4) Safety Inspections, and (5) Safety Inspection Checklist. The appendix features federal laws and regulations affecting laboratories. (YDS)

  12. Clinical efficacy, safety and anti-inflammatory activity of two sevelamer tablet forms in patients on low-flux hemodialysis.

    PubMed

    Tzanno-Martins, C; Biavo, B M M; Ferreira-Filho, O; Ribeiro-Junior, E; João-Luiz, M V S; Degaspari, S; Scavone, C; Kawamoto, E

    2014-01-01

    Sevelamer hydrochloride is an ionic exchange resin with high affinity for phosphate. This phosphate-binding agent has few serious adverse reactions with the advantage of reducing total and low density lipoprotein (LDL) cholesterol levels. However, it is controversial as to whether sevelamer hydrochloride can modulate the inflammatory response via endotoxin reduction. Therefore, a single-center, open-label, prospective and randomized study was performed to compare the clinical efficacy, safety and anti-inflammatory activity of two sevelamer hydrochloride tablet forms a branded tablet form, Renagel (Genzyme manufacturer) and its generic equivalent (EMS manufacturer). Twenty-eight chronic kidney disease volunteer patients at stage 5 (CDK 5D), on chronic low-flux hemodialysis carried out in 4-hour sessions, three times a week, were studied. The serum phosphorus, ionic calcium, total cholesterol and fractions, bicarbonate, blood pH, interleukin (IL)-6, IL-10, IL-1 beta and tumor necrosis factor-alpha (TNF-alpha) levels were collected prior to dialysis at mid-week. The incidence of gastrointestinal adverse effects were determined at the end of the phosphate-binder washout period as well as at the end of the fourth and eighth weeks of use of both tablet forms. The same magnitude of reduction in serum phosphorus was observed with both sevelamer tablet forms. Only the Renagel group showed lower total cholesterol and lower LDL cholesterol levels at the fourth and eighth week versus baseline. No significant differences in serum cytokine levels were identified in either drug group. However, the incidence of intestinal obstipation was higher among patients who used the generic equivalent form. In conclusion, Renagel and its EMS generic equivalent tablet forms have a similar clinical efficacy in reducing phosphorus in CKD 5D patients on low-flux hemodialysis and a similar safety profile. PMID:24674676

  13. Analgesic activity and safety of ash of silver used in Indian system of medicine in mice: A reverse pharmacological study

    PubMed Central

    Inder, Deep; Rehan, Harmeet Singh; Bajaj, Vijay Kumar; Kumar, Pawan; Gupta, Navin; Singh, Jasbir

    2012-01-01

    Objective: To study the analgesic activity of ash of silver used in Indian system of medicine and to explore its safety. Materials and Methods: Albino mice of either sex (20-30 gm) were used to investigate the role of ash of silver against noxious stimuli: thermal (Eddy's hot plate and analgesiometer), mechanical (tail clip), and chemical (0.6% acetic acid induced writhing). An effort was made to find nature and site of action of ash of silver following naloxone pre-treatment. Maximum tolerated dose (MTD) and lethal dosage 50 (LD50) were also studied along with toxicological aspects of ash of silver. Results: Test drug (ash of silver) at a dose of 50 mg/kg p.o exhibited analgesic activity against thermal, mechanical, and chemical stimuli. Analgesic effects were compared with the standard drug, morphine, in thermal and mechanical noxious stimuli and to aspirin in chemical stimulus. Analgesic activity of the test drug was reduced following naloxone pre-treatment. MTD was found out to be greater than 1.5 g/kg p.o. LD50 was 2 g/kg p.o. Fraction of mice showed symptoms of argyria as explained by autopsy reports. Conclusion: Test drug exhibited moderate analgesic activity at 50 mg/kg p.o against all type of noxious stimuli, also suggesting a role of opioidergic system. The ash of silver was been found to be safe upto a dose of 1.5 g/kg p.o. in mice without any untoward toxicity. Further studies are required to explore the effect of ash of silver on pain mediators and excitatory neurotransmitters like glutamate, aspartate, or N-methyl-D-aspartic acid (NMDA). PMID:22345869

  14. Subchronic oral toxicity and cardiovascular safety pharmacology studies of resveratrol, a naturally occurring polyphenol with cancer preventive activity

    PubMed Central

    Johnson, W.D.; Morrissey, R.L.; Usborne, A.L.; Kapetanovic, I.; Crowell, J.A.; Muzzio, M.; McCormick, D.L.

    2011-01-01

    To characterize the subchronic oral toxicity of resveratrol, CD rats received daily gavage doses of 0, 200, 400, or 1000 mg resveratrol/kg/day, and beagle dogs received daily capsule doses of 0, 200, 600, or 1200 mg resveratrol/kg/day for 90 days. Resveratrol induced only minimal toxicity, consisting of dose-related reductions in body weight gain in female rats and both sexes of dogs, and a statistically significant increase in bilirubin levels in rats at the 1000 mg/kg/day dose. Clinical observations, hematology, ophthalmology, neurotoxicity evaluations (functional observational batteries), organ weights, and gross pathology provided no biologically significant evidence of resveratrol toxicity in either species. In rats, the high dose of resveratrol reduced the incidence of cardiomyopathy; no other microscopic changes were seen. Histopathologic changes in dogs were limited to minimal inflammatory infiltrates in the kidney and urinary bladder, which were not considered toxicologically significant. A cardiovascular safety pharmacology (telemetry) study in dogs revealed no evidence of resveratrol toxicity. Based on body weight effects, the No Observed Adverse Effect Level (NOAEL) for resveratrol was 200 mg/kg/day in rats and 600 mg/kg/day in dogs. The apparent cardioprotective activity of resveratrol in rats demonstrates that its potentially beneficial activities may extend beyond efficacy in cancer prevention. PMID:21939727

  15. Eplerenone mimics features of the alternative activation in macrophages obtained from patients with heart failure and healthy volunteers.

    PubMed

    Łabuzek, Krzysztof; Liber, Sebastian; Bułdak, Łukasz; Krupej-Kędzierska, Joanna; Machnik, Grzegorz; Bobrzyk, Magdalena; Okopień, Bogusław

    2014-03-01

    Alternative activation of macrophages plays protective role in cardiac remodelling in heart failure and the activity of mineralocorticoid receptor may determine the phenotype of these cells. We examined the influence of eplerenone, aldosterone, and IL-4 on descriptors of alternative activation in blood monocytes collected from 19 patients with heart-failure and 20 healthy volunteers. “Heart failure” macrophages in comparison with “healthy” macrophages had increased mineralocorticoid activity, NO and reactive oxygen species production, expression of iNOS mRNA and protein, but decreased expression of arginase I and mannose receptor proteins, and activity of MnSOD and CuZnSOD. Aldosterone increased mineralocorticoid activity, NO and reactive oxygen species production, iNOS mRNA and protein expression, MnSOD and CuZnSOD activity. Eplerenone attenuated the effects of aldosterone on all but MnSOD and CuZnSOD variables. Eplerenone alone increased the production of NO, MnSOD and CuZnSOD activity, arginase I gene and protein expression, and mannose receptor gene and protein expression, but decreased mineralocorticoid activity only in “heart failure” macrophages. The latter suggests altered function of mineralocorticoid receptor in heart failure. Increased mineralocorticoid activity accounts for increased NO production, iNOS gene and protein expression but does not explain the increased basal reactive oxygen species production and decreased markers of alternative activation in “heart failure” macrophages. In the lack of change in basal mineralocorticoid activity, eplerenone increases markers of alternative activation in a mineralocorticoid receptor-independent manner. Because of changes in iNOS and NO variable, eplerenone induced qualitatively different activation of macrophages from that obtained with IL-4.

  16. Patient Safety: Ten Things You Can Do to Be a Safe Patient

    MedlinePlus

    ... Emergency Preparedness & Response Environmental Health Healthy Living Injury, Violence & Safety Life Stages & Populations Travelers' Health Workplace Safety & Health Features Media Sign up for Features Get Email Updates To ...

  17. Time-Frequency Feature Representation Using Multi-Resolution Texture Analysis and Acoustic Activity Detector for Real-Life Speech Emotion Recognition

    PubMed Central

    Wang, Kun-Ching

    2015-01-01

    The classification of emotional speech is mostly considered in speech-related research on human-computer interaction (HCI). In this paper, the purpose is to present a novel feature extraction based on multi-resolutions texture image information (MRTII). The MRTII feature set is derived from multi-resolution texture analysis for characterization and classification of different emotions in a speech signal. The motivation is that we have to consider emotions have different intensity values in different frequency bands. In terms of human visual perceptual, the texture property on multi-resolution of emotional speech spectrogram should be a good feature set for emotion classification in speech. Furthermore, the multi-resolution analysis on texture can give a clearer discrimination between each emotion than uniform-resolution analysis on texture. In order to provide high accuracy of emotional discrimination especially in real-life, an acoustic activity detection (AAD) algorithm must be applied into the MRTII-based feature extraction. Considering the presence of many blended emotions in real life, in this paper make use of two corpora of naturally-occurring dialogs recorded in real-life call centers. Compared with the traditional Mel-scale Frequency Cepstral Coefficients (MFCC) and the state-of-the-art features, the MRTII features also can improve the correct classification rates of proposed systems among different language databases. Experimental results show that the proposed MRTII-based feature information inspired by human visual perception of the spectrogram image can provide significant classification for real-life emotional recognition in speech. PMID:25594590

  18. Brain aneurysm segmentation in CTA and 3DRA using geodesic active regions based on second order prototype features and nonparametric density estimation

    NASA Astrophysics Data System (ADS)

    Hernandez, Monica; Frangi, Alejandro F.

    2005-04-01

    Coupling the geodesic active contours model with statistical information based on regions introduces robustness in the segmentation of images with weak or inhomogeneous gradients. In the estimation of the probability density function for each region take part the definition of the features that describe the image inside the different regions and the method of density estimation itself. A Gaussian Mixture Model is frequently proposed for density estimation. This approach is based on the assumption that the intensity distribution of the image is the most discriminant feature in a region. However, the use of second order features provides a better discrimination of the different regions, as these features represent more accurately the local properties of the image manifold. Due to the high dimensionality of the problem, the use of non parametric density estimation methods becomes necessary. In this article, we present a novel method of introducing the second order information of an image for non parametric estimation of the probability density functions of the different tissues that are present in medical images. The novelty of the method stems on the use of the response of the image under an orthogonal harmonic operator set projected onto a prototype space for feature generation. The technique described here is applied to the segmentation of brain aneurysms in Computed Tomography Angiography (CTA) and 3D Rotational Angiography (3DRA) showing a qualitative improvement from the Gaussian Mixture Model approach.

  19. Real-time evaluation of polyphenol oxidase (PPO) activity in lychee pericarp based on weighted combination of spectral data and image features as determined by fuzzy neural network.

    PubMed

    Yang, Yi-Chao; Sun, Da-Wen; Wang, Nan-Nan; Xie, Anguo

    2015-07-01

    A novel method of using hyperspectral imaging technique with the weighted combination of spectral data and image features by fuzzy neural network (FNN) was proposed for real-time prediction of polyphenol oxidase (PPO) activity in lychee pericarp. Lychee images were obtained by a hyperspectral reflectance imaging system operating in the range of 400-1000nm. A support vector machine-recursive feature elimination (SVM-RFE) algorithm was applied to eliminating variables with no or little information for the prediction from all bands, resulting in a reduced set of optimal wavelengths. Spectral information at the optimal wavelengths and image color features were then used respectively to develop calibration models for the prediction of PPO in pericarp during storage, and the results of two models were compared. In order to improve the prediction accuracy, a decision strategy was developed based on weighted combination of spectral data and image features, in which the weights were determined by FNN for a better estimation of PPO activity. The results showed that the combined decision model was the best among all of the calibration models, with high R(2) values of 0.9117 and 0.9072 and low RMSEs of 0.45% and 0.459% for calibration and prediction, respectively. These results demonstrate that the proposed weighted combined decision method has great potential for improving model performance. The proposed technique could be used for a better prediction of other internal and external quality attributes of fruits.

  20. Stimulus-related independent component and voxel-wise analysis of human brain activity during free viewing of a feature film.

    PubMed

    Lahnakoski, Juha M; Salmi, Juha; Jääskeläinen, Iiro P; Lampinen, Jouko; Glerean, Enrico; Tikka, Pia; Sams, Mikko

    2012-01-01

    Understanding how the brain processes stimuli in a rich natural environment is a fundamental goal of neuroscience. Here, we showed a feature film to 10 healthy volunteers during functional magnetic resonance imaging (fMRI) of hemodynamic brain activity. We then annotated auditory and visual features of the motion picture to inform analysis of the hemodynamic data. The annotations were fitted to both voxel-wise data and brain network time courses extracted by independent component analysis (ICA). Auditory annotations correlated with two independent components (IC) disclosing two functional networks, one responding to variety of auditory stimulation and another responding preferentially to speech but parts of the network also responding to non-verbal communication. Visual feature annotations correlated with four ICs delineating visual areas according to their sensitivity to different visual stimulus features. In comparison, a separate voxel-wise general linear model based analysis disclosed brain areas preferentially responding to sound energy, speech, music, visual contrast edges, body motion and hand motion which largely overlapped the results revealed by ICA. Differences between the results of IC- and voxel-based analyses demonstrate that thorough analysis of voxel time courses is important for understanding the activity of specific sub-areas of the functional networks, while ICA is a valuable tool for revealing novel information about functional connectivity which need not be explained by the predefined model. Our results encourage the use of naturalistic stimuli and tasks in cognitive neuroimaging to study how the brain processes stimuli in rich natural environments. PMID:22496909

  1. Stimulus-Related Independent Component and Voxel-Wise Analysis of Human Brain Activity during Free Viewing of a Feature Film

    PubMed Central

    Lahnakoski, Juha M.; Salmi, Juha; Jääskeläinen, Iiro P.; Lampinen, Jouko; Glerean, Enrico; Tikka, Pia; Sams, Mikko

    2012-01-01

    Understanding how the brain processes stimuli in a rich natural environment is a fundamental goal of neuroscience. Here, we showed a feature film to 10 healthy volunteers during functional magnetic resonance imaging (fMRI) of hemodynamic brain activity. We then annotated auditory and visual features of the motion picture to inform analysis of the hemodynamic data. The annotations were fitted to both voxel-wise data and brain network time courses extracted by independent component analysis (ICA). Auditory annotations correlated with two independent components (IC) disclosing two functional networks, one responding to variety of auditory stimulation and another responding preferentially to speech but parts of the network also responding to non-verbal communication. Visual feature annotations correlated with four ICs delineating visual areas according to their sensitivity to different visual stimulus features. In comparison, a separate voxel-wise general linear model based analysis disclosed brain areas preferentially responding to sound energy, speech, music, visual contrast edges, body motion and hand motion which largely overlapped the results revealed by ICA. Differences between the results of IC- and voxel-based analyses demonstrate that thorough analysis of voxel time courses is important for understanding the activity of specific sub-areas of the functional networks, while ICA is a valuable tool for revealing novel information about functional connectivity which need not be explained by the predefined model. Our results encourage the use of naturalistic stimuli and tasks in cognitive neuroimaging to study how the brain processes stimuli in rich natural environments. PMID:22496909

  2. Safety and Activity of Anti–PD-L1 Antibody in Patients with Advanced Cancer

    PubMed Central

    Brahmer, Julie R.; Tykodi, Scott S.; Chow, Laura Q.M.; Hwu, Wen-Jen; Topalian, Suzanne L.; Hwu, Patrick; Drake, Charles G.; Camacho, Luis H.; Kauh, John; Odunsi, Kunle; Pitot, Henry C.; Hamid, Omid; Bhatia, Shailender; Martins, Renato; Eaton, Keith; Chen, Shuming; Salay, Theresa M.; Alaparthy, Suresh; Grosso, Joseph F.; Korman, Alan J.; Parker, Susan M.; Agrawal, Shruti; Goldberg, Stacie M.; Pardoll, Drew M.; Gupta, Ashok; Wigginton, Jon M.

    2013-01-01

    BACKGROUND Programmed death 1 (PD-1) protein, a T-cell coinhibitory receptor, and one of its ligands, PD-L1, play a pivotal role in the ability of tumor cells to evade the host’s immune system. Blockade of interactions between PD-1 and PD-L1 enhances immune function in vitro and mediates antitumor activity in preclinical models. METHODS In this multicenter phase 1 trial, we administered intravenous anti–PD-L1 antibody (at escalating doses ranging from 0.3 to 10 mg per kilogram of body weight) to patients with selected advanced cancers. Anti–PD-L1 antibody was administered every 14 days in 6-week cycles for up to 16 cycles or until the patient had a complete response or confirmed disease progression. RESULTS As of February 24, 2012, a total of 207 patients — 75 with non–small-cell lung cancer, 55 with melanoma, 18 with colorectal cancer, 17 with renal-cell cancer, 17 with ovarian cancer, 14 with pancreatic cancer, 7 with gastric cancer, and 4 with breast cancer — had received anti–PD-L1 antibody. The median duration of therapy was 12 weeks (range, 2 to 111). Grade 3 or 4 toxic effects that investigators considered to be related to treatment occurred in 9% of patients. Among patients with a response that could be evaluated, an objective response (a complete or partial response) was observed in 9 of 52 patients with melanoma, 2 of 17 with renal-cell cancer, 5 of 49 with non–small-cell lung cancer, and 1 of 17 with ovarian cancer. Responses lasted for 1 year or more in 8 of 16 patients with at least 1 year of follow-up. CONCLUSIONS Antibody-mediated blockade of PD-L1 induced durable tumor regression (objective response rate of 6 to 17%) and prolonged stabilization of disease (rates of 12 to 41% at 24 weeks) in patients with advanced cancers, including non–small-cell lung cancer, melanoma, and renal-cell cancer. (Funded by Bristol-Myers Squibb and others; ClinicalTrials.gov number, NCT00729664.) PMID:22658128

  3. Pharmacodynamics, pharmacokinetics and safety of GSK2190915, a novel oral anti‐inflammatory 5‐lipoxygenase‐activating protein inhibitor

    PubMed Central

    Bain, Gretchen; King, Christopher D.; Schaab, Kevin; Rewolinski, Melissa; Norris, Virginia; Ambery, Claire; Bentley, Jane; Yamada, Masanori; Santini, Angelina M.; van de Wetering de Rooij, Jeroen; Stock, Nicholas; Zunic, Jasmine; Hutchinson, John H.; Evans, Jilly F.

    2013-01-01

    Aim To assess the pharmacokinetics, pharmacodynamics, safety and tolerability of the 5‐lipoxygenase‐activating protein inhibitor, GSK2190915, after oral dosing in two independent phase I studies, one in Western European and one in Japanese subjects, utilizing different formulations. Method Western European subjects received single (50–1000 mg) or multiple (10–450 mg) oral doses of GSK2190915 or placebo in a dose‐escalating manner. Japanese subjects received three of four GSK2190915 doses (10–200 mg) plus placebo once in a four period crossover design. Blood samples were collected for GSK2190915 concentrations and blood and urine were collected to measure leukotriene B4 and leukotriene E4, respectively, as pharmacodynamic markers of drug activity. Results There was no clear difference in adverse events between placebo and active drug‐treated subjects in either study. Maximum plasma concentrations of GSK2190915 and area under the curve increased in a dose‐related manner and mean half‐life values ranged from 16–34 h. Dose‐dependent inhibition of blood leukotriene B4 production was observed and near complete inhibition of urinary leukotriene E4 excretion was shown at all doses except the lowest dose. The EC50 values for inhibition of LTB4 were 85 nm and 89 nm in the Western European and Japanese studies, respectively. Conclusion GSK2190915 is well‐tolerated with pharmacokinetics and pharmacodynamics in Western European and Japanese subjects that support once daily dosing for 24 h inhibition of leukotrienes. Doses of ≥50 mg show near complete inhibition of urinary leukotriene E4 at 24 h post‐dose, whereas doses of ≥150 mg are required for 24 h inhibition of blood LTB4. PMID:22803688

  4. Pharmacokinetics, Pharmacodynamics, Safety, and Clinical Activity of Multiple Doses of RCT-18 in Chinese Patients With Systemic Lupus Erythematosus.

    PubMed

    Zhao, Qian; Chen, Xia; Hou, Yong; Jiang, Ji; Zhong, Wen; Yao, Xuejing; Wang, Wenxiang; Li, Lin; Fang, Jianmin; Zhang, Fengchun; Hu, Pei

    2016-08-01

    RCT-18 is a novel recombinant fusion protein that blocks the activity of a B-lymphocyte stimulator and a proliferation-inducing ligand. This was a randomized, single-blind, and placebo-controlled phase 1 study in 12 patients with systemic lupus erythematosus. Eligible patients were randomized 3:1 to receive multiple subcutaneous doses of RCT-18 for 4 weeks (180 mg, once weekly) or placebo and monitored over an 84-day observation period for pharmacokinetics, pharmacodynamics, immunogenicity, safety, and clinical activity. After multiple-dose RCT-18, the maximal serum concentration (Cmax ) of total and free RCT-18 was reached within 1 to 2 days. Mean elimination half-life for total RCT-18 and free RCT-18 was 11.4 to 26.4 days and 2.4 to 26.5 days, respectively. Slight accumulation was found after multiple subcutaneous administrations. The average accumulation ratios of AUC and Cmax after the fourth administration of RCT-18 were 2.0 and 1.7 for total RCT-18, and 1.8 and 1.6 for free RCT-18. The formation and elimination of BLyS-RCT-18 complex were much slower, with a time to Cmax of 14 to 46 days. Pharmacokinetic characteristics of RCT-18 in SLE patients were similar to those in patients with rheumatoid arthritis. No positive reaction was detected in the immunogenicity assessments. RCT-18 was biologically active, according to serum immunoglobulin and B-cell levels. Treatment-related IgM and IgA reduction was found during this study. CD19(+) , IgD(+) , and CD27(+) B-cell counts were increased after administration and decreased subsequently. SLE patients treated with RCT-18 were more prone to infections, including moderate and severe infections. Lower dosages of RCT-18 should be considered in further clinical development.

  5. [Changes in acetylcholinesterase and ATPase activity and certain structural features of the erythrocyte membrane in experimental myocardial ischemia].

    PubMed

    Chernukh, A M; Kopteva, L A; Shevchenko, A S

    1980-09-01

    Acute heart ischemia induced by ligation of the left coronary artery is associated with variation in the activity of acetylcholinesterase, Na, K-ATPase and Ca, Mg-ATPase in rat erythrocytes. The maximum pronounced variations in the enzymatic activity and in the membrane capacity of erythroblasts for binding direct turquoise are recorded on the 7th day of the experimental myocardial infarction.

  6. Incidental and Context-Responsive Activation of Structure- and Function-Based Action Features during Object Identification

    ERIC Educational Resources Information Center

    Lee, Chia-lin; Middleton, Erica; Mirman, Daniel; Kalenine, Solene; Buxbaum, Laurel J.

    2013-01-01

    Previous studies suggest that action representations are activated during object processing, even when task-irrelevant. In addition, there is evidence that lexical-semantic context may affect such activation during object processing. Finally, prior work from our laboratory and others indicates that function-based ("use") and structure-based…

  7. Characterization of the Etna volcanic emissions through an active biomonitoring technique (moss-bags): part 2--morphological and mineralogical features.

    PubMed

    Calabrese, S; D'Alessandro, W

    2015-01-01

    Volcanic emissions were studied at Mount Etna (Italy) by using moss-bags technique. Mosses were exposed around the volcano at different distances from the active vents to evaluate the impact of volcanic emissions in the atmosphere. Morphology and mineralogy of volcanic particulate intercepted by mosses were investigated using scanning electron microscopy (SEM) equipped with energy dispersive spectrometer (EDS). Particles emitted during passive degassing activity from the two active vents, Bocca Nuova and North East Crater (BNC and NEC), were identified as silicates, sulfates and halide compounds. In addition to volcanic particles, we found evidences also of geogenic, anthropogenic and marine spray input. The study has shown the robustness of this active biomonitoring technique to collect particles, very useful in active volcanic areas characterized by continuous degassing and often not easily accessible to apply conventional sampling techniques.

  8. Characterization of the Etna volcanic emissions through an active biomonitoring technique (moss-bags): part 2--morphological and mineralogical features.

    PubMed

    Calabrese, S; D'Alessandro, W

    2015-01-01

    Volcanic emissions were studied at Mount Etna (Italy) by using moss-bags technique. Mosses were exposed around the volcano at different distances from the active vents to evaluate the impact of volcanic emissions in the atmosphere. Morphology and mineralogy of volcanic particulate intercepted by mosses were investigated using scanning electron microscopy (SEM) equipped with energy dispersive spectrometer (EDS). Particles emitted during passive degassing activity from the two active vents, Bocca Nuova and North East Crater (BNC and NEC), were identified as silicates, sulfates and halide compounds. In addition to volcanic particles, we found evidences also of geogenic, anthropogenic and marine spray input. The study has shown the robustness of this active biomonitoring technique to collect particles, very useful in active volcanic areas characterized by continuous degassing and often not easily accessible to apply conventional sampling techniques. PMID:25311770

  9. Students' Perceptions of Patient Safety during the Transition from Undergraduate to Postgraduate Training: An Activity Theory Analysis

    ERIC Educational Resources Information Center

    de Feijter, Jeantine M.; de Grave, Willem S.; Dornan, Tim; Koopmans, Richard P.; Scherpbier, Albert J. J. A.

    2011-01-01

    Evidence that medical error can cause harm to patients has raised the attention of the health care community towards patient safety and influenced how and what medical students learn about it. Patient safety is best taught when students are participating in clinical practice where they actually encounter patients at risk. This type of learning is…

  10. Volcanic features of Io

    USGS Publications Warehouse

    Carr, M.H.; Masursky, H.; Strom, R.G.; Terrile, R.J.

    1979-01-01

    Volcanic activity is apparently higher on Io than on any other body in the Solar System. Its volcanic landforms can be compared with features on Earth to indicate the type of volcanism present on Io. ?? 1979 Nature Publishing Group.

  11. Decisions about Product Safety. A Multidisciplinary Teaching Unit.

    ERIC Educational Resources Information Center

    Procter and Gamble Educational Services, Cincinnati, OH.

    A multidisciplinary educational unit featuring product safety decisions made by businesses and consumers is presented. teach critical thinking and decision making while supplementing the science, home economics, social studies, and economics curricula. The activities rely extensively, though not exclusively, on Procter & Gamble's experiences in…

  12. Structural features and complement-fixing activity of pectin from three Brassica oleracea varieties: white cabbage, kale, and red kale.

    PubMed

    Samuelsen, Anne Berit; Westereng, Bjørge; Yousif, Osman; Holtekjølen, Ann Katrin; Michaelsen, Terje E; Knutsen, Svein H

    2007-02-01

    Leaves of different cabbage species are used both as food and as wound healing remedies in traditional medicine. This supposed wound healing activity might be connected to presence of immunomodulating water soluble polysaccharides. To study this, three different cabbage varieties, white cabbage (W), kale (K), and red kale (RK), were pretreated with 80% ethanol and then extracted with water at 50 degrees C and 100 degrees C for isolation of polysaccharide-containing fractions. The fractions were analyzed for monosaccharide composition, glycosidic linkages, Mw distribution, protein content, and phenolic compounds and then tested for complement-fixing activity. All fractions contained pectin type polysaccharides with linkages corresponding to homogalacturonan and hairy regions. Those extracted at 50 degrees C contained higher amounts of neutral side chains and were more active in the complement-fixation test than those extracted at 100 degrees C. The fractions can be ranged by decreasing activity: K-50 > RK-50 > W-50 approximately = K-100 > RK100 approximately = W-100. Studies on structure-activity relationships (SAR) employing multivariate statistical analysis strongly suggest that the magnitude of the measured activity is influenced by the content of certain side chains in the polymers. High activity correlates to large neutral side chains with high amounts of (1-->6)- and (1-->3,6)-linked Gal and low amounts of (1-->4)-linked GalA but not on molecular weight distribution of the polymers.

  13. A Review of Botanical Characteristics, Traditional Usage, Chemical Components, Pharmacological Activities, and Safety of Pereskia bleo (Kunth) DC.

    PubMed

    Zareisedehizadeh, Sogand; Tan, Chay-Hoon; Koh, Hwee-Ling

    2014-01-01

    Pereskia bleo, a leafy cactus, is a medicinal plant native to West and South America and distributed in tropical and subtropical areas. It is traditionally used as a dietary vegetable, barrier hedge, water purifier, and insect repellant and for maintaining health, detoxification, prevention of cancer, and/or treatment of cancer, hypertension, diabetes, stomach ache, muscle pain, and inflammatory diseases such as dermatitis and rheumatism. The aim of this paper was to provide an up-to-date and comprehensive review of the botanical characteristics, traditional usage, phytochemistry, pharmacological activities, and safety of P. bleo. A literature search using MEDLINE (via PubMed), Science direct, Scopus and Google scholar and China Academic Journals Full-Text Database (CNKI) and available eBooks and books in the National University of Singapore libraries in English and Chinese was conducted. The following keywords were used: Pereskia bleo, Pereskia panamensis, Pereskia corrugata, Rhodocacus corrugatus, Rhodocacus bleo, Cactus panamensis, Cactus bleo, Spinach cactus, wax rose, Perescia, and Chinese rose. This review revealed the association between the traditional usage of P. bleo and reported pharmacological properties in the literature. Further investigation on the pharmacological properties and phytoconstituents of P. bleo is warranted to further exploit its potentials as a source of novel therapeutic agents or lead compounds. PMID:24987426

  14. Effects of airflow rates and operator activity on containment of bacterial aerosols in a class II safety cabinet.

    PubMed Central

    Macher, J M; First, M W

    1984-01-01

    Biological safety cabinets are frequently relied upon to provide sterile work environments in which hazardous microorganisms can be safely handled. Verification of correct airstream velocities does not, by itself, ensure that adequate protection will be achieved under all users. Instead, the concentration of microorganisms in a cabinet operator's breathing zone must be measured during typical cabinet use conditions to determine whether the exposure is below acceptable limits. In this study, cabinet operator exposures were measured with a personal air sampler. Bacterial spores were released inside a cabinet as a uniform challenge aerosol, and the number of escaping spores was measured for several cabinet arrangements during a number of typical operations. The following were studied to determine their effects on aerosol containment: inflow air velocity, size of access opening, type of operator movements, location of operator's hands, and pace of activity. Other experiments examined differences in aerosol containment for eight typical microbiology operations when performed by six operators who covered a range of body heights and volumes. PMID:6437327

  15. A Review of Botanical Characteristics, Traditional Usage, Chemical Components, Pharmacological Activities, and Safety of Pereskia bleo (Kunth) DC

    PubMed Central

    Tan, Chay-Hoon

    2014-01-01

    Pereskia bleo, a leafy cactus, is a medicinal plant native to West and South America and distributed in tropical and subtropical areas. It is traditionally used as a dietary vegetable, barrier hedge, water purifier, and insect repellant and for maintaining health, detoxification, prevention of cancer, and/or treatment of cancer, hypertension, diabetes, stomach ache, muscle pain, and inflammatory diseases such as dermatitis and rheumatism. The aim of this paper was to provide an up-to-date and comprehensive review of the botanical characteristics, traditional usage, phytochemistry, pharmacological activities, and safety of P. bleo. A literature search using MEDLINE (via PubMed), Science direct, Scopus and Google scholar and China Academic Journals Full-Text Database (CNKI) and available eBooks and books in the National University of Singapore libraries in English and Chinese was conducted. The following keywords were used: Pereskia bleo, Pereskia panamensis, Pereskia corrugata, Rhodocacus corrugatus, Rhodocacus bleo, Cactus panamensis, Cactus bleo, Spinach cactus, wax rose, Perescia, and Chinese rose. This review revealed the association between the traditional usage of P. bleo and reported pharmacological properties in the literature. Further investigation on the pharmacological properties and phytoconstituents of P. bleo is warranted to further exploit its potentials as a source of novel therapeutic agents or lead compounds. PMID:24987426

  16. A Review of Botanical Characteristics, Traditional Usage, Chemical Components, Pharmacological Activities, and Safety of Pereskia bleo (Kunth) DC.

    PubMed

    Zareisedehizadeh, Sogand; Tan, Chay-Hoon; Koh, Hwee-Ling

    2014-01-01

    Pereskia bleo, a leafy cactus, is a medicinal plant native to West and South America and distributed in tropical and subtropical areas. It is traditionally used as a dietary vegetable, barrier hedge, water purifier, and insect repellant and for maintaining health, detoxification, prevention of cancer, and/or treatment of cancer, hypertension, diabetes, stomach ache, muscle pain, and inflammatory diseases such as dermatitis and rheumatism. The aim of this paper was to provide an up-to-date and comprehensive review of the botanical characteristics, traditional usage, phytochemistry, pharmacological activities, and safety of P. bleo. A literature search using MEDLINE (via PubMed), Science direct, Scopus and Google scholar and China Academic Journals Full-Text Database (CNKI) and available eBooks and books in the National University of Singapore libraries in English and Chinese was conducted. The following keywords were used: Pereskia bleo, Pereskia panamensis, Pereskia corrugata, Rhodocacus corrugatus, Rhodocacus bleo, Cactus panamensis, Cactus bleo, Spinach cactus, wax rose, Perescia, and Chinese rose. This review revealed the association between the traditional usage of P. bleo and reported pharmacological properties in the literature. Further investigation on the pharmacological properties and phytoconstituents of P. bleo is warranted to further exploit its potentials as a source of novel therapeutic agents or lead compounds.

  17. Safety, formulation, and in vitro antiviral activity of the antimicrobial peptide subtilosin against herpes simplex virus type 1

    PubMed Central

    Torres, Nicolás I.; Noll, Katia Sutyak; Xu, Shiqi; Li, Ji; Huang, Qingrong; Sinko, Patrick J.; Wachsman, Mónica B.; Chikindas, Michael L.

    2013-01-01

    In the present study the antiviral properties of the bacteriocin subtilosin against Herpes simplex virus type 1 (HSV-1) and the safety and efficacy of a subtilosin-based nanofiber formulation were determined. High concentrations of subtilosin, the cyclical antimicrobial peptide produced by Bacillus amyloliquefaciens, were virucidal against HSV-1. Interestingly, at non-virucidal concentrations, subtilosin inhibited wild type HSV-1 and aciclovir-resistant mutants in a dose-dependent manner. Although the exact antiviral mechanism is not fully understood, time of addition experiments and western blot analysis suggest that subtilosin does not affect viral multiplication steps prior to protein synthesis. Poly(vinyl alcohol) (PVOH)-based subtilosin nanofibers with a width of 278 nm were produced by the electrospinning process. The retained antimicrobial activity of the subtilosin-based fibers was determined via an agar well diffusion assay. The loading capacity of the fibers was 2.4 mg subtilosin/g fiber, and loading efficiency was 31.6%. Furthermore, the nanofibers with and without incorporated subtilosin were shown to be nontoxic to human epidermal tissues using an in vitro human tissue model. Taking together these results subtilosin-based nanofibers should be further studied as a novel alternative method for treatment and/or control of HSV-1 infection. PMID:23637711

  18. You've found a safety signal--now what?: regulatory implications of industry signal detection activities.

    PubMed

    Kahn, Sidney N

    2007-01-01

    Signals detected by measuring disproportionality of drug-event combinations are only statistical indicators of possible real safety issues, and are not per se necessarily medically important. Nevertheless, once a signal is observed, sponsors are obligated by regulations and ethical considerations to determine whether it represents a new product-associated risk by additional analysis, validation and evaluation of its clinical relevance. Signal strength does not necessarily correlate with medical significance. Strong signals most often represent known, expected and/or medically trivial adverse reactions or confounding by treatment indication, common co-morbidities or other common concomitant treatments. Conversely, any product with reasonably extensive clinical use and reporting of suspected adverse reactions is likely to manifest many weak but clinically unimportant signals, creating significant background 'noise'. Since relatively rare, medically important adverse drug reactions are often likely to manifest as weak signals, sponsors face a potentially onerous burden of evaluating multiple signals in order to distinguish true, clinically important events of concern from spurious signals. This paper discusses the regulatory, clinical and potential legal liability issues that confront industry as a consequence of signal identification activities, including: current and anticipated regulatory requirements for detection, assessment and reporting; the reliability of the data used for signal generation; assessment of clinical relevance; organisational approaches and responses to observed signals; targeted clinical and scientific responses to observed signals; and potential regulatory, legal and commercial impact.

  19. Where’s the Noise? Key Features of Spontaneous Activity and Neural Variability Arise through Learning in a Deterministic Network

    PubMed Central

    Hartmann, Christoph; Lazar, Andreea; Nessler, Bernhard; Triesch, Jochen

    2015-01-01

    Even in the absence of sensory stimulation the brain is spontaneously active. This background “noise” seems to be the dominant cause of the notoriously high trial-to-trial variability of neural recordings. Recent experimental observations have extended our knowledge of trial-to-trial variability and spontaneous activity in several directions: 1. Trial-to-trial variability systematically decreases following the onset of a sensory stimulus or the start of a motor act. 2. Spontaneous activity states in sensory cortex outline the region of evoked sensory responses. 3. Across development, spontaneous activity aligns itself with typical evoked activity patterns. 4. The spontaneous brain activity prior to the presentation of an ambiguous stimulus predicts how the stimulus will be interpreted. At present it is unclear how these observations relate to each other and how they arise in cortical circuits. Here we demonstrate that all of these phenomena can be accounted for by a deterministic self-organizing recurrent neural network model (SORN), which learns a predictive model of its sensory environment. The SORN comprises recurrently coupled populations of excitatory and inhibitory threshold units and learns via a combination of spike-timing dependent plasticity (STDP) and homeostatic plasticity mechanisms. Similar to balanced network architectures, units in the network show irregular activity and variable responses to inputs. Additionally, however, the SORN exhibits sequence learning abilities matching recent findings from visual cortex and the network’s spontaneous activity reproduces the experimental findings mentioned above. Intriguingly, the network’s behaviour is reminiscent of sampling-based probabilistic inference, suggesting that correlates of sampling-based inference can develop from the interaction of STDP and homeostasis in deterministic networks. We conclude that key observations on spontaneous brain activity and the variability of neural responses can be

  20. Where's the Noise? Key Features of Spontaneous Activity and Neural Variability Arise through Learning in a Deterministic Network.

    PubMed

    Hartmann, Christoph; Lazar, Andreea; Nessler, Bernhard; Triesch, Jochen

    2015-12-01

    Even in the absence of sensory stimulation the brain is spontaneously active. This background "noise" seems to be the dominant cause of the notoriously high trial-to-trial variability of neural recordings. Recent experimental observations have extended our knowledge of trial-to-trial variability and spontaneous activity in several directions: 1. Trial-to-trial variability systematically decreases following the onset of a sensory stimulus or the start of a motor act. 2. Spontaneous activity states in sensory cortex outline the region of evoked sensory responses. 3. Across development, spontaneous activity aligns itself with typical evoked activity patterns. 4. The spontaneous brain activity prior to the presentation of an ambiguous stimulus predicts how the stimulus will be interpreted. At present it is unclear how these observations relate to each other and how they arise in cortical circuits. Here we demonstrate that all of these phenomena can be accounted for by a deterministic self-organizing recurrent neural network model (SORN), which learns a predictive model of its sensory environment. The SORN comprises recurrently coupled populations of excitatory and inhibitory threshold units and learns via a combination of spike-timing dependent plasticity (STDP) and homeostatic plasticity mechanisms. Similar to balanced network architectures, units in the network show irregular activity and variable responses to inputs. Additionally, however, the SORN exhibits sequence learning abilities matching recent findings from visual cortex and the network's spontaneous activity reproduces the experimental findings mentioned above. Intriguingly, the network's behaviour is reminiscent of sampling-based probabilistic inference, suggesting that correlates of sampling-based inference can develop from the interaction of STDP and homeostasis in deterministic networks. We conclude that key observations on spontaneous brain activity and the variability of neural responses can be

  1. Where's the Noise? Key Features of Spontaneous Activity and Neural Variability Arise through Learning in a Deterministic Network.

    PubMed

    Hartmann, Christoph; Lazar, Andreea; Nessler, Bernhard; Triesch, Jochen

    2015-12-01

    Even in the absence of sensory stimulation the brain is spontaneously active. This background "noise" seems to be the dominant cause of the notoriously high trial-to-trial variability of neural recordings. Recent experimental observations have extended our knowledge of trial-to-trial variability and spontaneous activity in several directions: 1. Trial-to-trial variability systematically decreases following the onset of a sensory stimulus or the start of a motor act. 2. Spontaneous activity states in sensory cortex outline the region of evoked sensory responses. 3. Across development, spontaneous activity aligns itself with typical evoked activity patterns. 4. The spontaneous brain activity prior to the presentation of an ambiguous stimulus predicts how the stimulus will be interpreted. At present it is unclear how these observations relate to each other and how they arise in cortical circuits. Here we demonstrate that all of these phenomena can be accounted for by a deterministic self-organizing recurrent neural network model (SORN), which learns a predictive model of its sensory environment. The SORN comprises recurrently coupled populations of excitatory and inhibitory threshold units and learns via a combination of spike-timing dependent plasticity (STDP) and homeostatic plasticity mechanisms. Similar to balanced network architectures, units in the network show irregular activity and variable responses to inputs. Additionally, however, the SORN exhibits sequence learning abilities matching recent findings from visual cortex and the network's spontaneous activity reproduces the experimental findings mentioned above. Intriguingly, the network's behaviour is reminiscent of sampling-based probabilistic inference, suggesting that correlates of sampling-based inference can develop from the interaction of STDP and homeostasis in deterministic networks. We conclude that key observations on spontaneous brain activity and the variability of neural responses can be

  2. Drug Safety

    MedlinePlus

    ... over-the-counter drug. The FDA evaluates the safety of a drug by looking at Side effects ... clinical trials The FDA also monitors a drug's safety after approval. For you, drug safety means buying ...

  3. [Features of the lipid peroxidation process and antioxidant activity of white rats in extreme, multi-stage supercooling].

    PubMed

    Serebrennikova, E G; Mamaev, A T; Akhmedov, I G

    1992-01-01

    High content of polyenic fatty acids, slight increase in antioxidative activity and decrease in content of malonic dialdehyde (MDA) in rat myocardial tissues were observed in supercooling. The data obtained suggest that intensive oxidation of fatty acids occurred in the tissues, which may be essential for synthesis of biologically active substances involved in development of resistance to supercooling. Multiple supercooling caused an opposite effect in lung tissue: saturation of lipids was increased, antioxidative activity was maintained on the level similar to the control values, while content of MDA was markedly increased apparently due to destruction of metabolic products penetrating from other tissues. The dissimilar impairments of lipid metabolism were detected in liver tissue, where multiple supercooling did not alter the rate of lipid saturation, antioxidative activity was unaltered and content of MDA was as low as in the control animals.

  4. Efficacy, Safety and Anticancer Activity of Protein Nanoparticle-Based Delivery of Doxorubicin through Intravenous Administration in Rats

    PubMed Central

    Golla, Kishore; Cherukuvada, Bhaskar; Ahmed, Farhan; Kondapi, Anand K.

    2012-01-01

    Background and Aims Doxorubicin is a potent anticancer drug and a major limiting factor that hinders therapeutic use as its high levels of systemic circulation often associated with various off-target effects, particularly cardiotoxicity. The present study focuses on evaluation of the efficacy of doxorubicin when it is loaded into the protein nanoparticles and delivered intravenously in rats bearing Hepatocellular carcinoma (HCC). The proteins selected as carrier were Apotransferrin and Lactoferrin, since the receptors for these two proteins are known to be over expressed on cancer cells due to their iron transport capacity. Methods Doxorubicin loaded apotransferrin (Apodoxonano) and lactoferrin nanoparticles (Lactodoxonano) were prepared by sol-oil chemistry. HCC in the rats was induced by 100 mg/l of diethylnitrosamine (DENA) in drinking water for 8 weeks. Rats received 5 doses of 2 mg/kg drug equivalent nanoparticles through intravenous administration. Pharmacokinetics and toxicity of nanoformulations was evaluated in healthy rats and anticancer activity was studied in DENA treated rats. The anticancer activity was evaluated through counting of the liver nodules, H & E analysis and by estimating the expression levels of angiogenic and antitumor markers. Results In rats treated with nanoformulations, the numbers of liver nodules were found to be significantly reduced. They showed highest drug accumulation in liver (22.4 and 19.5 µg/g). Both nanoformulations showed higher localization compared to doxorubicin (Doxo) when delivered in the absence of a carrier. Higher amounts of Doxo (195 µg/g) were removed through kidney, while Apodoxonano and Lactodoxonano showed only a minimal amount of removal (<40 µg/g), suggesting the extended bioavailability of Doxo when delivered through nanoformulation. Safety analysis shows minimal cardiotoxicity due to lower drug accumulation in heart in the case of nanoformulation. Conclusion Drug delivery through nanoformulations not

  5. Efficacy and safety of low-dose tacrolimus for active rheumatoid arthritis with an inadequate response to methotrexate

    PubMed Central

    Lee, Won-Seok; Lee, Sang-Il; Lee, Myeung-Soo; Kim, Sung-Il; Lee, Shin-Seok; Yoo, Wan-Hee

    2016-01-01

    Background/Aims: To determine the efficacy and safety of low-dose tacrolimus in Korean rheumatoid arthritis (RA) subjects with an inadequate response to methotrexate (MTX). Methods: This was a multicenter, open-label study conducted at five Korean sites. Fifty-six patients with active RA, despite treatment for ≥ 1 month with a stable, maximally tolerated dosage of oral MTX (median dosage, 15 mg/wk), were enrolled and received 1.5 mg/day of tacrolimus as a single oral dose once per day for 16 weeks while continuing to receive MTX. All other disease-modifying anti-rheumatic drugs were discontinued, whereas stable dosages of nonsteroidal anti-inflammatory drugs and oral corticosteroids (≤ 10 mg/day of prednisone or an equivalent corticosteroid) were allowed. The primary clinical response criterion was the American College of Rheumatology’s definition of 20% improvement (ACR20) at the end of treatment. Results: The ACR20 response rate was 42.9% (24 of 56 patients) in patients who had received tacrolimus at least once. The overall ACR50 and ACR70 responses at the end of treatment for all patients were 30.4% and 10.7%, respectively. Throughout the treatment period, 37 patients experienced 71 adverse events (AEs) in total, and four patients left the study because of AEs. In addition, 15 patients in total experienced treatment-related AEs. Throughout the treatment period, two patients were reported to experience two serious AEs, and one patient left the study because of a serious AE. Conclusions: In patients whose active RA persists despite treatment with MTX, low-dose tacrolimus in combination with MTX appears to be safe and well tolerated, and provides clinical benefit. PMID:26961485

  6. The relationship between the lizard eye and associated bony features: a cautionary note for interpreting fossil activity patterns.

    PubMed

    Hall, Margaret I

    2009-06-01

    Activity pattern, the time of day when an animal is active, is associated with ecology. There are two major activity patterns: diurnal (awake during the day in a photopic environment) and nocturnal (awake at night in a scotopic environment). Lizards exhibit characteristic eye shapes associated with activity pattern, with scotopic-adapted lizard eyes optimized for visual sensitivity with large corneal diameters relative to their eye axial lengths, and photopic-adapted lizards optimized for visual acuity, with larger axial lengths of the eye relative to their corneal diameters. This study: (1) quantifies the relationship between the lizard eye and its associated bony anatomy (the orbit, sclerotic ring, and associated skull widths); (2) investigates how activity pattern is reflected in that bony anatomy; and (3) determines if it is possible to reliably interpret activity pattern for a lizard that does not have the soft tissue available for study, specifically, for a fossil. Knowledge of extinct lizards' activity patterns would be useful in making paleoecological interpretations. Here, 96 scotopic- and photopic-adapted lizard species are analyzed in a phylogenetic context. Although there is a close relationship between the lepidosaur eye and associated bony anatomy, based on these data activity pattern cannot be reliably interpreted for bony-only specimens, such as a fossil, possibly because of the limited ossification of the lepidosaur skull. Caution should be exercised when utilizing lizard bony anatomy to interpret light-level adaptation, either for a fossil lizard or as part of an extant phylogenetic bracket to interpret other extinct animals with sclerotic rings, such as dinosaurs. PMID:19462447

  7. Aerospace Safety Advisory Panel

    NASA Technical Reports Server (NTRS)

    1992-01-01

    The results of the Panel's activities are presented in a set of findings and recommendations. Highlighted here are both improvements in NASA's safety and reliability activities and specific areas where additional gains might be realized. One area of particular concern involves the curtailment or elimination of Space Shuttle safety and reliability enhancements. Several findings and recommendations address this area of concern, reflecting the opinion that safety and reliability enhancements are essential to the continued successful operation of the Space Shuttle. It is recommended that a comprehensive and continuing program of safety and reliability improvements in all areas of Space Shuttle hardware/software be considered an inherent component of ongoing Space Shuttle operations.

  8. Interactive effect of salicylic acid on some physiological features and antioxidant enzymes activity in ginger (Zingiber officinale Roscoe).

    PubMed

    Ghasemzadeh, Ali; Jaafar, Hawa Z E

    2013-01-01

    The effect of foliar salicylic acid (SA) applications (10⁻³ and 10⁻⁵ M) on activities of nitrate reductase, guaiacol peroxidase (POD), superoxide dismutases (SOD), catalase (CAT) and proline enzymes and physiological parameters was evaluated in two ginger varieties (Halia Bentong and Halia Bara) under greenhouse conditions. In both varieties, tested treatments generally enhanced photosynthetic rate and total dry weight. Photosynthetic rate increases were generally accompanied by increased or unchanged stomatal conductance levels, although intercellular CO₂ concentrations of treated plants were typically lower than in controls. Lower SA concentrations were generally more effective in enhancing photosynthetic rate and plant growth. Exogenous application of SA increased antioxidant enzyme activities and proline content; the greatest responses were obtained in plants sprayed with 10⁻⁵ M SA, with significant increases observed in CAT (20.1%), POD (45.2%), SOD (44.1%) and proline (43.1%) activities. Increased CAT activity in leaves is naturally expected to increase photosynthetic efficiency and thus net photosynthesis by maintaining a constant CO₂ supply. Our results support the idea that low SA concentrations (10⁻⁵ M) may induce nitrite reductase synthesis by mobilizing intracellular NO³⁻ and can provide protection to nitrite reductase degradation in vivo in the absence of NO³⁻. Observed positive correlations among proline, SOD, CAT and POD activities in the studied varieties suggest that increased SOD activity was accompanied by increases in CAT and POD activities because of the high demands of H₂O₂ quenching. PMID:23698049

  9. Featured Article: Differential regulation of endothelial nitric oxide synthase phosphorylation by protease-activated receptors in adult human endothelial cells

    PubMed Central

    Tillery, Lakeisha C; Epperson, Tenille A; Eguchi, Satoru

    2016-01-01

    Protease-activated receptors have been shown to regulate endothelial nitric oxide synthase through the phosphorylation of specific sites on the enzyme. It has been established that PAR-2 activation phosphorylates eNOS-Ser-1177 and leads to the production of the potent vasodilator nitric oxide, while PAR-1 activation phosphorylates eNOS-Thr-495 and decreases nitric oxide production in human umbilical vein endothelial cells. In this study, we hypothesize a differential coupling of protease-activated receptors to the signaling pathways that regulates endothelial nitric oxide synthase and nitric oxide production in primary adult human coronary artery endothelial cells. Using Western Blot analysis, we showed that thrombin and the PAR-1 activating peptide, TFLLR, lead to the phosphorylation of eNOS-Ser-1177 in human coronary artery endothelial cells, which was blocked by SCH-79797 (SCH), a PAR-1 inhibitor. Using the nitrate/nitrite assay, we also demonstrated that the thrombin- and TFLLR-induced production of nitric oxide was inhibited by SCH and L-NAME, a NOS inhibitor. In addition, we observed that TFLLR, unlike thrombin, significantly phosphorylated eNOS-Thr-495, which may explain the observed delay in nitric oxide production in comparison to that of thrombin. Activation of PAR-2 by SLIGRL, a PAR-2 specific ligand, leads to dual phosphorylation of both catalytic sites but primarily regulated eNOS-Thr-495 phosphorylation with no change in nitric oxide production in human coronary artery endothelial cells. PAR-3, known as the non-signaling receptor, was activated by TFRGAP, a PAR-3 mimicking peptide, and significantly induced the phosphorylation of eNOS-Thr-495 with minimal phosphorylation of eNOS-Ser-1177 with no change in nitric oxide production. In addition, we confirmed that PAR-mediated eNOS-Ser-1177 phosphorylation was Ca2+-dependent using the Ca2+ chelator, BAPTA, while eNOS-Thr-495 phosphorylation was mediated via Rho kinase using the ROCK inhibitor, Y-27632

  10. Featured Article: Differential regulation of endothelial nitric oxide synthase phosphorylation by protease-activated receptors in adult human endothelial cells.

    PubMed

    Tillery, Lakeisha C; Epperson, Tenille A; Eguchi, Satoru; Motley, Evangeline D

    2016-03-01

    Protease-activated receptors have been shown to regulate endothelial nitric oxide synthase through the phosphorylation of specific sites on the enzyme. It has been established that PAR-2 activation phosphorylates eNOS-Ser-1177 and leads to the production of the potent vasodilator nitric oxide, while PAR-1 activation phosphorylates eNOS-Thr-495 and decreases nitric oxide production in human umbilical vein endothelial cells. In this study, we hypothesize a differential coupling of protease-activated receptors to the signaling pathways that regulates endothelial nitric oxide synthase and nitric oxide production in primary adult human coronary artery endothelial cells. Using Western Blot analysis, we showed that thrombin and the PAR-1 activating peptide, TFLLR, lead to the phosphorylation of eNOS-Ser-1177 in human coronary artery endothelial cells, which was blocked by SCH-79797 (SCH), a PAR-1 inhibitor. Using the nitrate/nitrite assay, we also demonstrated that the thrombin- and TFLLR-induced production of nitric oxide was inhibited by SCH and L-NAME, a NOS inhibitor. In addition, we observed that TFLLR, unlike thrombin, significantly phosphorylated eNOS-Thr-495, which may explain the observed delay in nitric oxide production in comparison to that of thrombin. Activation of PAR-2 by SLIGRL, a PAR-2 specific ligand, leads to dual phosphorylation of both catalytic sites but primarily regulated eNOS-Thr-495 phosphorylation with no change in nitric oxide production in human coronary artery endothelial cells. PAR-3, known as the non-signaling receptor, was activated by TFRGAP, a PAR-3 mimicking peptide, and significantly induced the phosphorylation of eNOS-Thr-495 with minimal phosphorylation of eNOS-Ser-1177 with no change in nitric oxide production. In addition, we confirmed that PAR-mediated eNOS-Ser-1177 phosphorylation was Ca(2+)-dependent using the Ca(2+) chelator, BAPTA, while eNOS-Thr-495 phosphorylation was mediated via Rho kinase using the ROCK inhibitor, Y-27632

  11. Safety Control and Safety Education at Technical Institutes

    NASA Astrophysics Data System (ADS)

    Iino, Hiroshi

    The importance of safety education for students at technical institutes is emphasized on three grounds including safety of all working members and students in their education, research and other activities. The Kanazawa Institute of Technology re-organized the safety organization into a line structure and improved safety minds of all their members and now has a chemical materials control system and a set of compulsory safety education programs for their students, although many problems still remain.

  12. NASA Software Safety Standard

    NASA Technical Reports Server (NTRS)

    Rosenberg, Linda

    1997-01-01

    If software is a critical element in a safety critical system, it is imperative to implement a systematic approach to software safety as an integral part of the overall system safety programs. The NASA-STD-8719.13A, "NASA Software Safety Standard", describes the activities necessary to ensure that safety is designed into software that is acquired or developed by NASA, and that safety is maintained throughout the software life cycle. A PDF version, is available on the WWW from Lewis. A Guidebook that will assist in the implementation of the requirements in the Safety Standard is under development at the Lewis Research Center (LeRC). After completion, it will also be available on the WWW from Lewis.

  13. Deciphering solar magnetic activity. I. On the relationship between the sunspot cycle and the evolution of small magnetic features

    SciTech Connect

    McIntosh, Scott W.; Wang, Xin; Markel, Robert S.; Thompson, Michael J.; Leamon, Robert J.; Malanushenko, Anna V.; Davey, Alisdair R.; Howe, Rachel; Krista, Larisza D.; Cirtain, Jonathan W.; Gurman, Joseph B.; Pesnell, William D.

    2014-09-01

    Sunspots are a canonical marker of the Sun's internal magnetic field which flips polarity every ∼22 yr. The principal variation of sunspots, an ∼11 yr variation, modulates the amount of the magnetic field that pierces the solar surface and drives significant variations in our star's radiative, particulate, and eruptive output over that period. This paper presents observations from the Solar and Heliospheric Observatory and Solar Dynamics Observatory indicating that the 11 yr sunspot variation is intrinsically tied to the spatio-temporal overlap of the activity bands belonging to the 22 yr magnetic activity cycle. Using a systematic analysis of ubiquitous coronal brightpoints and the magnetic scale on which they appear to form, we show that the landmarks of sunspot cycle 23 can be explained by considering the evolution and interaction of the overlapping activity bands of the longer-scale variability.

  14. Quantitatively analyzing metathesis catalyst activity and structural features in silica-supported tungsten imido-alkylidene complexes.

    PubMed

    Mougel, Victor; Santiago, Celine B; Zhizhko, Pavel A; Bess, Elizabeth N; Varga, Jeno; Frater, Georg; Sigman, Matthew S; Copéret, Christophe

    2015-05-27

    A broad series of fully characterized, well-defined silica-supported W metathesis catalysts with the general formula [(≡SiO)W(═NAr)(═CHCMe2R)(X)] (Ar = 2,6-iPr2C6H3 (AriPr), 2,6-Cl2C6H3 (ArCl), 2-CF3C6H4 (ArCF3), and C6F5 (ArF5); X = OC(CF3)3 (OtBuF9), OCMe(CF3)2 (OtBuF6), OtBu, OSi(OtBu)3, 2,5-dimethylpyrrolyl (Me2Pyr) and R = Me or Ph) was prepared by grafting bis-X substituted complexes [W(NAr)(═CHCMe2R)(X)2] on silica partially dehydroxylated at 700 °C (SiO2-(700)), and their activity was evaluated with the goal to obtain detailed structure-activity relationships. Quantitative influence of the ligand set on the activity (turnover frequency, TOF) in self-metathesis of cis-4-nonene was investigated using multivariate linear regression analysis tools. The TOF of these catalysts (activity) can be well predicted from simple steric and electronic parameters of the parent protonated ligands; it is described by the mutual contribution of the NBO charge of the nitrogen or the IR intensity of the symmetric N-H stretch of the ArNH2, corresponding to the imido ligand, together with the Sterimol B5 and pKa of HX, representing the X ligand. This quantitative and predictive structure-activity relationship analysis of well-defined heterogeneous catalysts shows that high activity is associated with the combination of X and NAr ligands of opposite electronic character and paves the way toward rational development of metathesis catalysts.

  15. A tale of two itches. Common features and notable differences in brain activation evoked by cowhage and histamine induced itch.

    PubMed

    Papoiu, Alexandru D P; Coghill, Robert C; Kraft, Robert A; Wang, Hui; Yosipovitch, Gil

    2012-02-15

    Previous PET and fMRI brain imaging studies targeting neural networks processing itch sensation have used histamine as the sole itch inducer. In contrast with histamine, cowhage-induced itch is mediated via proteinase activated receptors PAR2 and is transmitted through a separate spinothalamic pathway, therefore imaging the brain activation evoked by cowhage could provide further insight into central processing of itch. We report for the first time a functional MRI Arterial Spin Labeling (ASL) study of neuronal processing of itch induced by cowhage, analyzed in contrast with histamine-induced itch. We also explored the brain responses induced by histamine and cowhage combined in a tight sequence. The results of our analyses obtained in a group of 15 healthy volunteers suggested that cowhage and histamine co-activated a core group of brain structures, while also revealing notable differences. Core areas activated by both stimuli were found in the thalamus, primary and secondary somatosensory cortices, posterior parietal cortex, superior and middle temporal cortices, PCC, ACC, precuneus and cuneus. Cowhage induced a notably distinct and more extensive involvement of the insular cortex, claustrum, basal ganglia, putamen, thalamic nuclei and pulvinar. The differences observed between these two itch modalities were investigated to determine the impact of quantitative versus qualitative factors, and correlations between itch intensity and the patterns in brain activation were explored. Our analysis revealed that the most significant differences between cowhage and histamine itch were not affected by stimulus intensity, although a subset of regions displayed activations which were intensity-dependent. The combined application of cowhage and histamine highlighted the role of insula and claustrum in the processing of both itch modalities in the same time. The present results suggest the existence of overlapping but also distinct neuronal networks processing these two

  16. Application of genetic algorithm-kernel partial least square as a novel nonlinear feature selection method: activity of carbonic anhydrase II inhibitors.

    PubMed

    Jalali-Heravi, Mehdi; Kyani, Anahita

    2007-05-01

    This paper introduces the genetic algorithm-kernel partial least square (GA-KPLS), as a novel nonlinear feature selection method. This technique combines genetic algorithms (GAs) as powerful optimization methods with KPLS as a robust nonlinear statistical method for variable selection. This feature selection method is combined with artificial neural network to develop a nonlinear QSAR model for predicting activities of a series of substituted aromatic sulfonamides as carbonic anhydrase II (CA II) inhibitors. Eight simple one- and two-dimensional descriptors were selected by GA-KPLS and considered as inputs for developing artificial neural networks (ANNs). These parameters represent the role of acceptor-donor pair, hydrogen bonding, hydrosolubility and lipophilicity of the active sites and also the size of the inhibitors on inhibitor-isozyme interaction. The accuracy of 8-4-1 networks was illustrated by validation techniques of leave-one-out (LOO) and leave-multiple-out (LMO) cross-validations and Y-randomization. Superiority of this method (GA-KPLS-ANN) over the linear one (MLR) in a previous work and also the GA-PLS-ANN in which a linear feature selection method has been used indicates that the GA-KPLS approach is a powerful method for the variable selection in nonlinear systems. PMID:17316919

  17. Molecular pathway activation features linked with transition from normal skin to primary and metastatic melanomas in human.

    PubMed

    Shepelin, Denis; Korzinkin, Mikhail; Vanyushina, Anna; Aliper, Alexander; Borisov, Nicolas; Vasilov, Raif; Zhukov, Nikolay; Sokov, Dmitry; Prassolov, Vladimir; Gaifullin, Nurshat; Zhavoronkov, Alex; Bhullar, Bhupinder; Buzdin, Anton

    2016-01-01

    Melanoma is the most aggressive and dangerous type of skin cancer, but its molecular mechanisms remain largely unclear. For transcriptomic data of 478 primary and metastatic melanoma, nevi and normal skin samples, we performed high-throughput analysis of intracellular molecular networks including 592 signaling and metabolic pathways. We showed that at the molecular pathway level, the formation of nevi largely resembles transition from normal skin to primary melanoma. Using a combination of bioinformatic machine learning algorithms, we identified 44 characteristic signaling and metabolic pathways connected with the formation of nevi, development of primary melanoma, and its metastases. We created a model describing formation and progression of melanoma at the level of molecular pathway activation. We discovered six novel associations between activation of metabolic molecular pathways and progression of melanoma: for allopregnanolone biosynthesis, L-carnitine biosynthesis, zymosterol biosynthesis (inhibited in melanoma), fructose 2, 6-bisphosphate synthesis and dephosphorylation, resolvin D biosynthesis (activated in melanoma), D-myo-inositol hexakisphosphate biosynthesis (activated in primary, inhibited in metastatic melanoma). Finally, we discovered fourteen tightly coordinated functional clusters of molecular pathways. This study helps to decode molecular mechanisms underlying the development of melanoma. PMID:26624979

  18. Molecular pathway activation features linked with transition from normal skin to primary and metastatic melanomas in human

    PubMed Central

    Shepelin, Denis; Korzinkin, Mikhail; Vanyushina, Anna; Aliper, Alexander; Borisov, Nicolas; Vasilov, Raif; Zhukov, Nikolay; Sokov, Dmitry; Prassolov, Vladimir; Gaifullin, Nurshat; Zhavoronkov, Alex; Bhullar, Bhupinder; Buzdin, Anton

    2016-01-01

    Melanoma is the most aggressive and dangerous type of skin cancer, but its molecular mechanisms remain largely unclear. For transcriptomic data of 478 primary and metastatic melanoma, nevi and normal skin samples, we performed high-throughput analysis of intracellular molecular networks including 592 signaling and metabolic pathways. We showed that at the molecular pathway level, the formation of nevi largely resembles transition from normal skin to primary melanoma. Using a combination of bioinformatic machine learning algorithms, we identified 44 characteristic signaling and metabolic pathways connected with the formation of nevi, development of primary melanoma, and its metastases. We created a model describing formation and progression of melanoma at the level of molecular pathway activation. We discovered six novel associations between activation of metabolic molecular pathways and progression of melanoma: for allopregnanolone biosynthesis, L-carnitine biosynthesis, zymosterol biosynthesis (inhibited in melanoma), fructose 2, 6-bisphosphate synthesis and dephosphorylation, resolvin D biosynthesis (activated in melanoma), D-myo-inositol hexakisphosphate biosynthesis (activated in primary, inhibited in metastatic melanoma). Finally, we discovered fourteen tightly coordinated functional clusters of molecular pathways. This study helps to decode molecular mechanisms underlying the development of melanoma. PMID:26624979

  19. Molecular pathway activation features linked with transition from normal skin to primary and metastatic melanomas in human.

    PubMed

    Shepelin, Denis; Korzinkin, Mikhail; Vanyushina, Anna; Aliper, Alexander; Borisov, Nicolas; Vasilov, Raif; Zhukov, Nikolay; Sokov, Dmitry; Prassolov, Vladimir; Gaifullin, Nurshat; Zhavoronkov, Alex; Bhullar, Bhupinder; Buzdin, Anton

    2016-01-01

    Melanoma is the most aggressive and dangerous type of skin cancer, but its molecular mechanisms remain largely unclear. For transcriptomic data of 478 primary and metastatic melanoma, nevi and normal skin samples, we performed high-throughput analysis of intracellular molecular networks including 592 signaling and metabolic pathways. We showed that at the molecular pathway level, the formation of nevi largely resembles transition from normal skin to primary melanoma. Using a combination of bioinformatic machine learning algorithms, we identified 44 characteristic signaling and metabolic pathways connected with the formation of nevi, development of primary melanoma, and its metastases. We created a model describing formation and progression of melanoma at the level of molecular pathway activation. We discovered six novel associations between activation of metabolic molecular pathways and progression of melanoma: for allopregnanolone biosynthesis, L-carnitine biosynthesis, zymosterol biosynthesis (inhibited in melanoma), fructose 2, 6-bisphosphate synthesis and dephosphorylation, resolvin D biosynthesis (activated in melanoma), D-myo-inositol hexakisphosphate biosynthesis (activated in primary, inhibited in metastatic melanoma). Finally, we discovered fourteen tightly coordinated functional clusters of molecular pathways. This study helps to decode molecular mechanisms underlying the development of melanoma.

  20. Safety, Tolerability & Potential Anti-cancer Activity of Increasing Doses of AZD5363 in Different Treatment Schedules

    ClinicalTrials.gov

    2016-10-26

    Advanced Solid Malignancy; Safety and Tolerability; Pharmacokinetics; Pharmacodynamics; Tumour Response; Advanced or Metastatic Breast Cancer; Ovarian Cancer; Cervical Cancer; Endometrial Cancer; PIK3CA; AKT1; PTEN; ER Positive; HER2 Positive

  1. Natural killer cell dysfunction is a distinguishing feature of systemic onset juvenile rheumatoid arthritis and macrophage activation syndrome.

    PubMed

    Villanueva, Joyce; Lee, Susan; Giannini, Edward H; Graham, Thomas B; Passo, Murray H; Filipovich, Alexandra; Grom, Alexei A

    2005-01-01

    Macrophage activation syndrome (MAS) has been reported in association with many rheumatic diseases, most commonly in systemic juvenile rheumatoid arthritis (sJRA). Clinically, MAS is similar to hemophagocytic lymphohistiocytosis (HLH), a genetic disorder with absent or depressed natural killer (NK) function. We have previously reported that, as in HLH, patients with MAS have profoundly decreased NK activity, suggesting that this abnormality might be relevant to the pathogenesis of the syndrome. Here we examined the extent of NK dysfunction across the spectrum of diseases that comprise juvenile rheumatoid arthritis (JRA). Peripheral blood mononuclear cells (PBMC) were collected from patients with pauciarticular (n = 4), polyarticular (n = 16), and systemic (n = 20) forms of JRA. NK cytolytic activity was measured after co-incubation of PBMC with the NK-sensitive K562 cell line. NK cells (CD56+/T cell receptor [TCR]-alphabeta-), NK T cells (CD56+/TCR-alphabeta+), and CD8+ T cells were also assessed for perforin and granzyme B expression by flow cytometry. Overall, NK cytolytic activity was significantly lower in patients with sJRA than in other JRA patients and controls. In a subgroup of patients with predominantly sJRA, NK cell activity was profoundly decreased: in 10 of 20 patients with sJRA and in only 1 of 20 patients with other JRA, levels of NK activity were below two standard deviations of pediatric controls (P = 0.002). Some decrease in perforin expression in NK cells and cytotoxic T lymphocytes was seen in patients within each of the JRA groups with no statistically significant differences. There was a profound decrease in the proportion of circulating CD56bright NK cells in three sJRA patients, a pattern similar to that previously observed in MAS and HLH. In conclusion, a subgroup of patients with JRA who have not yet had an episode of MAS showed decreased NK function and an absence of circulating CD56bright population, similar to the abnormalities observed

  2. Thermal reactor safety

    SciTech Connect

    Not Available

    1980-06-01

    Information is presented concerning new trends in licensing; seismic considerations and system structural behavior; TMI-2 risk assessment and thermal hydraulics; statistical assessment of potential accidents and verification of computational methods; issues with respect to improved safety; human factors in nuclear power plant operation; diagnostics and activities in support of recovery; LOCA transient analysis; unresolved safety issues and other safety considerations; and fission product transport.

  3. Statistical properties of sunspot, active region distributions and flare occurrences in the cycle 23 as detected from the Solar Feature Catalogues

    NASA Astrophysics Data System (ADS)

    Zharkova, V. V.; Zharkov, S. I.

    2006-08-01

    The statistical properties of sunspot and active regions (plages) distributions and their relation to flare distributions are presented for 1996-2005 from the automated Solar Feature Catalogues from the SOHO and Meudon Observatories (SFC, http://solar.inf.brad.ac.uk). Cross-correlation analysis is carried out between flare sizes, locations, significance and active region/sunspot parameters including magnetic field extracted in SFC. Sunspot and plage area distributions reveals a strong North-South asymmetry of about 0.2 and the period of about 7-8 years for sunspots and of 0.5 and period of 9 years for plages with both asymmetries decreasing towards the next cycle minimum. The temporal distribution of solar flare occurrences in Northern and Southern hemispheres, at different latitudes and longitudes are compared with those of plage and sunspot areas and LOS magnetic fields. The application of these results to the solar activity forecast is discussed.

  4. Polyalkoxybenzenes from plants. 5. Parsley seed extract in synthesis of azapodophyllotoxins featuring strong tubulin destabilizing activity in the sea urchin embryo and cell culture assays.

    PubMed

    Semenova, Marina N; Kiselyov, Alex S; Tsyganov, Dmitry V; Konyushkin, Leonid D; Firgang, Sergei I; Semenov, Roman V; Malyshev, Oleg R; Raihstat, Mikhail M; Fuchs, Fabian; Stielow, Anne; Lantow, Margareta; Philchenkov, Alex A; Zavelevich, Michael P; Zefirov, Nikolay S; Kuznetsov, Sergei A; Semenov, Victor V

    2011-10-27

    A series of 4-azapodophyllotoxin derivatives with modified rings B and E have been synthesized using allylpolyalkoxybenzenes from parsley seed oil. The targeted molecules were evaluated in vivo in a phenotypic sea urchin embryo assay for antimitotic and tubulin destabilizing activity. The most active compounds identified by the in vivo sea urchin embryo assay featured myristicin-derived ring E. These molecules were determined to be more potent than podophyllotoxin. Cytotoxic effects of selected molecules were further confirmed and evaluated by conventional assays with A549 and Jurkat human leukemic T-cell lines including cell growth inhibition, cell cycle arrest, cellular microtubule disruption, and induction of apoptosis. The ring B modification yielded 6-OMe substituted molecule as the most active compound. Finally, in Jurkat cells, compound induced caspase-dependent apoptosis mediated by the apical caspases-2 and -9 and not caspase-8, implying the involvement of the intrinsic caspase-9-dependent apoptotic pathway.

  5. Evidence for safety of Ayurvedic herbal, herbo-metallic and Bhasma preparations on neurobehavioral activity and oxidative stress in rats

    PubMed Central

    Kumar, Gajendra; Gupta, Yogendra Kumar

    2012-01-01

    Heavy metals in Ayurvedic formulations have been used for centuries with claimed efficacy and safety. However, concerns are often raised about the toxicity due to heavy metals used in Ayurvedic formulations. The aim of present study is to explore the effect of Calcury tablet, Energic-31 capsule and Basanta Kusumakara Rasa (BKR) on neurobehavioral activity and oxidative stress in rats. Male wistar rats weighing 200-250 g were used and divided into normal control, positive control (mercury chloride, lead acetate, cadmium chloride, sodium arsenite, each 10 mg/kg, p.o for 28 days) and treated group (Calcury tablets at doses of 130, 650, 1300 mg/kg, Energic-31 capsule at doses of 150, 750, 1500 mg/kg and BKR at doses of 26, 130, 260 mg/kg, p.o. for 28 days). After performing the behavioural parameters on the 29th day, homogenate of rat's brain was used to determine malondialdehyde (MDA) and glutathione (GSH) levels and heavy metal level in brain. Results showed that there were no significant change in cognitive function, motor coordination, MDA and GSH levels as compared to normal control group at all doses of Calcury tablet, Energic-31 capsule and Basant Kusumkar Rasa. However, heavy metals level in rat's brain was higher as compared to normal control group at all doses of Calcury tablet, Energic-31 capsule and BKR. In conclusion, Calcury tablet, Energic-31 capsule and BKR in doses equivalent to the human dose does not have appreciable adverse effects on brain which demonstrates the non-toxic nature of metal based Ayurvedic formulations. PMID:23723678

  6. Swollenin from Trichoderma reesei exhibits hydrolytic activity against cellulosic substrates with features of both endoglucanases and cellobiohydrolases.

    PubMed

    Andberg, Martina; Penttilä, Merja; Saloheimo, Markku

    2015-04-01

    The cellulolytic and hemicellulolytic enzymes of Trichoderma reesei comprise one of the best characterised enzyme systems involved in lignocellulose degradation. In this paper, swollenin (SWOI), a protein recognised based on its sequence similarity with plant expansins, has been characterised. SWOI and its catalytic domain were subjected to analysis of their hydrolytic activity on different soluble carbohydrate polymers. By measuring the production of reducing ends, zymogram-, and viscosity analysis, SWOI was shown to have activity on substrates containing β-1,4 glucosidic bonds, i.e. carboxymethyl cellulose, hydroxyethyl cellulose and β-glucan. The formation of oligosaccharides from β-glucan was analysed by HPLC and showed cellobiose as the main reaction product. SWOI was also able to hydrolyse soluble cello-oligosaccharides and the products formed were all consistent with SWOI cleaving a cellobiose unit off the substrate. In conclusion, the T. reesei swollenin showed a unique mode of action with similarities with action of both endoglucanases and cellobiohydrolases. PMID:25643956

  7. Structural features related to hydrolytic activity against ceftazidime of plasmid-mediated SHV-type CAZ-5 beta-lactamase.

    PubMed Central

    Péduzzi, J; Barthélémy, M; Tiwari, K; Mattioni, D; Labia, R

    1989-01-01

    Tryptic peptides of the novel ceftazidimase CAZ-5 were sequenced by manual Edman degradation and aligned according to strong homology (more than 98%) with SHV-1 and SHV-2 beta-lactamase sequences. CAZ-5 differed from SHV-1 by five amino acid substitutions. Unusually high activity of CAZ-5 towards ceftazidime was imputed to substitution of a Lys for a Glu at position 214 of the mature protein. PMID:2694955

  8. Recent innovations in IFR safety research

    SciTech Connect

    Wade, D.C.

    1994-03-01

    Recent progress in IFR safety research suggests potential for two extensions of passive features to improve the robustness of safety response. This report provides a discussion of these recent innovations.

  9. Integration of Nevada Test Site (NTS) Work Control Programs and Incorporating Integrated Safety Management (ISM) into Activity Level Work Planning and Control

    SciTech Connect

    Mike Kinney and Kevin Breen

    2008-08-30

    This session will examine a method developed by Federal and Contractor personnel at the Nevada Site Office (NSO) to improve the planning and execution of work activities utilizing an Activity Level Work Control process in response to Defense Nuclear Facilities Safety Board (DNFSB) Recommendation 2004-1, Oversight of Complex, High-Hazard Nuclear Operations. The process was initially developed during Fiscal Year (FY) 2007, and implementation is commencing during the fourth quarter of FY 2008. This process will significantly enhance the flexibility and the appropriate rigor in the performance of work activities.

  10. fMRI Activity in Posterior Parietal Cortex Relates to the Perceptual Use of Binocular Disparity for Both Signal-In-Noise and Feature Difference Tasks

    PubMed Central

    Patten, Matthew L.; Welchman, Andrew E.

    2015-01-01

    Visually guided action and interaction depends on the brain’s ability to (a) extract and (b) discriminate meaningful targets from complex retinal inputs. Binocular disparity is known to facilitate this process, and it is an open question how activity in different parts of the visual cortex relates to these fundamental visual abilities. Here we examined fMRI responses related to performance on two different tasks (signal-in-noise “coarse” and feature difference “fine” tasks) that have been widely used in previous work, and are believed to differentially target the visual processes of signal extraction and feature discrimination. We used multi-voxel pattern analysis to decode depth positions (near vs. far) from the fMRI activity evoked while participants were engaged in these tasks. To look for similarities between perceptual judgments and brain activity, we constructed ‘fMR-metric’ functions that described decoding performance as a function of signal magnitude. Thereafter we compared fMR-metric and psychometric functions, and report an association between judged depth and fMRI responses in the posterior parietal cortex during performance on both tasks. This highlights common stages of processing during perceptual performance on these tasks. PMID:26529314

  11. Investing in International Information Exchange Activities to Improve the Safety, Cost Effectiveness and Schedule of Cleanup - 13281

    SciTech Connect

    Seed, Ian; James, Paula; Mathieson, John; Judd, Laurie; Elmetti-Ramirez, Rosa; Han, Ana

    2013-07-01

    With decreasing budgets and increasing pressure on completing cleanup missions as quickly, safely and cost-effectively as possible, there is significant benefit to be gained from collaboration and joint efforts between organizations facing similar issues. With this in mind, the US Department of Energy (DOE) and the UK Nuclear Decommissioning Authority (NDA) have formally agreed to share information on lessons learned on the development and application of new technologies and approaches to improve the safety, cost effectiveness and schedule of the cleanup legacy wastes. To facilitate information exchange a range of tools and methodologies were established. These included tacit knowledge exchange through facilitated meetings, conference calls and Site visits as well as explicit knowledge exchange through document sharing and newsletters. A DOE web-based portal has been established to capture these exchanges and add to them via discussion boards. The information exchange is operating at the Government-to-Government strategic level as well as at the Site Contractor level to address both technical and managerial topic areas. This effort has resulted in opening a dialogue and building working relationships. In some areas joint programs of work have been initiated thus saving resource and enabling the parties to leverage off one another activities. The potential benefits of high quality information exchange are significant, ranging from cost avoidance through identification of an approach to a problem that has been proven elsewhere to cost sharing and joint development of a new technology to address a common problem. The benefits in outcomes significantly outweigh the costs of the process. The applicability of the tools and methods along with the lessons learned regarding some key issues is of use to any organization that wants to improve value for money. In the waste management marketplace, there are a multitude of challenges being addressed by multiple organizations and

  12. Safety, Pharmacokinetics, Pharmacodynamics, and Activity of Navitoclax, a Targeted High Affinity Inhibitor of BCL-2, in Lymphoid Malignancies

    PubMed Central

    Wilson, Wyndham H.; O’Connor, Owen A.; Czuczman, Myron S.; LaCasce, Ann S.; Gerecitano, John F.; Leonard, John P.; Tulpule, Anil; Dunleavy, Kieron; Xiong, Hao; Chiu, Yi-Lin; Cui, Yue; Busman, Todd; Elmore, Steven W.; Rosenberg, Saul H.; Krivoshik, Andrew P.; Enschede, Sari H.; Humerickhouse, Rod A.

    2010-01-01

    SUMMARY Background BCL-2 family proteins play a central role in regulating clonal selection and survival of lymphocytes and are frequently over expressed in lymphomas. Navitoclax (ABT-263) is a targeted high-affinity small molecule that occupies the BH3 binding groove of BCL-2 and BCL-XL and inhibits their anti-apoptotic activity. Experimentally, navitoclax kills cells in a BAX/BAK-dependent manner and results in regression of lymphoid tumors in xenograft models. Methods This is a phase I dose-escalation study of navitoclax in patients with relapsed or refractory lymphoid malignancies. Study endpoints included safety, maximum tolerated dose (MTD), pharmacokinetic profile and clinical activity. In addition, mechanism-based pharmacodynamic effects on platelets and lymphocytes were assessed. Navitoclax was orally administered and assessed on an intermittent schedule of once daily for 14 days followed by 7 days off (14/21 days) or on a continuous once daily schedule (21/21 days). This trial is registered with ClinicalTrials.gov, number NCT00406809. Findings Fifty-five patients were enrolled, (median age 59 years, IQR 51–67), of whom two did not complete the first cycle and were not evaluable for assessment of dose-limiting toxicity (DLT). Common toxicities included grade 1/2 diarrhea and fatigue in 31 and 21 patients, respectively. Thrombocytopenia and neutropenia were the serious common toxicities with grade 3/4 observed in 29 and 17 patients, respectively. On the intermittent schedule (14/21), 5 DLT’s were observed; two due to hospitalizations for bronchitis and pleural effusion, and one each due to grade 3 transaminase elevation, grade 4 thrombocytopenia and grade 3 cardiac arrhythmia. Navitoclax caused a rapid and dose-dependent decline in peripheral platelets following initial drug exposure, followed by a rebound. To reduce the platelet nadir associated with intermittent dosing, a lead-in dose followed by continuous dosing (21/21 schedule) was examined. Three

  13. Identification of benzopyrone as a common structural feature in compounds with anti-inflammatory activity in a zebrafish phenotypic screen

    PubMed Central

    Robertson, Anne L.; Ogryzko, Nikolay V.; Henry, Katherine M.; Loynes, Catherine A.; Foulkes, Matthew J.; Meloni, Marco M.; Wang, Xingang; Ford, Christopher; Jackson, Malcolm; Ingham, Philip W.; Wilson, Heather L.; Farrow, Stuart N.; Solari, Roberto; Flower, Roderick J.; Jones, Simon; Whyte, Moira K. B.

    2016-01-01

    ABSTRACT Neutrophils are essential for host defence and are recruited to sites of inflammation in response to tissue injury or infection. For inflammation to resolve, these cells must be cleared efficiently and in a controlled manner, either by apoptosis or reverse migration. If the inflammatory response is not well-regulated, persistent neutrophils can cause damage to host tissues and contribute to the pathogenesis of chronic inflammatory diseases, which respond poorly to current treatments. It is therefore important to develop drug discovery strategies that can identify new therapeutics specifically targeting neutrophils, either by promoting their clearance or by preventing their recruitment. Our recent in vivo chemical genetic screen for accelerators of inflammation resolution identified a subset of compounds sharing a common chemical signature, the bicyclic benzopyrone rings. Here, we further investigate the mechanisms of action of the most active of this chemical series, isopimpinellin, in our zebrafish model of neutrophilic inflammation. We found that this compound targets both the recruitment and resolution phases of the inflammatory response. Neutrophil migration towards a site of injury is reduced by isopimpinellin and this occurs as a result of PI3K inhibition. We also show that isopimpinellin induces neutrophil apoptosis to drive inflammation resolution in vivo using a new zebrafish reporter line detecting in vivo neutrophil caspase-3 activity and allowing quantification of flux through the apoptotic pathway in real time. Finally, our studies reveal that clinically available ‘cromones’ are structurally related to isopimpinellin and have previously undescribed pro-resolution activity in vivo. These findings could have implications for the therapeutic use of benzopyrones in inflammatory disease. PMID:27079522

  14. Characterization and Comparison of the Structural Features, Immune-Modulatory and Anti-Avian Influenza Virus Activities Conferred by Three Algal Sulfated Polysaccharides.

    PubMed

    Song, Lin; Chen, Xiaolin; Liu, Xiaodong; Zhang, Fubo; Hu, Linfeng; Yue, Yang; Li, Kecheng; Li, Pengcheng

    2015-12-29

    Three marine macroalgae, i.e., Grateloupia filicina, Ulva pertusa and Sargassum qingdaoense, were selected as the deputies of Rhodophyta, Chlorophyta and Ochrophyta for comparative analysis of the molecular structures and biological activities of sulfated polysaccharides (SP). The ratio of water-soluble polysaccharides, the monosaccharide composition and the sulfated contents of three extracted SPs were determined, and their structures were characterized by Fourier transformation infrared spectroscopy. In addition, biological activity analysis showed that all three SPs had immune-modulatory activity both in vitro and in vivo, and SPs from S. qingdaoense had the best effect. Further bioassays showed that three SPs could not only enhance the immunity level stimulated by inactivated avian influenza virus (AIV) in vivo but also significantly inhibited the activity of activated AIV (H9N2 subtype) in vitro. G. filicina SP exhibited the strongest anti-AIV activity. These results revealed the variations in structural features and bioactivities among three SPs and indicated the potential adjuvants for immune-enhancement and anti-AIV.

  15. Characterization and Comparison of the Structural Features, Immune-Modulatory and Anti-Avian Influenza Virus Activities Conferred by Three Algal Sulfated Polysaccharides

    PubMed Central

    Song, Lin; Chen, Xiaolin; Liu, Xiaodong; Zhang, Fubo; Hu, Linfeng; Yue, Yang; Li, Kecheng; Li, Pengcheng

    2015-01-01

    Three marine macroalgae, i.e., Grateloupia filicina, Ulva pertusa and Sargassum qingdaoense, were selected as the deputies of Rhodophyta, Chlorophyta and Ochrophyta for comparative analysis of the molecular structures and biological activities of sulfated polysaccharides (SP). The ratio of water-soluble polysaccharides, the monosaccharide composition and the sulfated contents of three extracted SPs were determined, and their structures were characterized by Fourier transformation infrared spectroscopy. In addition, biological activity analysis showed that all three SPs had immune-modulatory activity both in vitro and in vivo, and SPs from S. qingdaoense had the best effect. Further bioassays showed that three SPs could not only enhance the immunity level stimulated by inactivated avian influenza virus (AIV) in vivo but also significantly inhibited the activity of activated AIV (H9N2 subtype) in vitro. G. filicina SP exhibited the strongest anti-AIV activity. These results revealed the variations in structural features and bioactivities among three SPs and indicated the potential adjuvants for immune-enhancement and anti-AIV. PMID:26729137

  16. Characterization and Comparison of the Structural Features, Immune-Modulatory and Anti-Avian Influenza Virus Activities Conferred by Three Algal Sulfated Polysaccharides.

    PubMed

    Song, Lin; Chen, Xiaolin; Liu, Xiaodong; Zhang, Fubo; Hu, Linfeng; Yue, Yang; Li, Kecheng; Li, Pengcheng

    2016-01-01

    Three marine macroalgae, i.e., Grateloupia filicina, Ulva pertusa and Sargassum qingdaoense, were selected as the deputies of Rhodophyta, Chlorophyta and Ochrophyta for comparative analysis of the molecular structures and biological activities of sulfated polysaccharides (SP). The ratio of water-soluble polysaccharides, the monosaccharide composition and the sulfated contents of three extracted SPs were determined, and their structures were characterized by Fourier transformation infrared spectroscopy. In addition, biological activity analysis showed that all three SPs had immune-modulatory activity both in vitro and in vivo, and SPs from S. qingdaoense had the best effect. Further bioassays showed that three SPs could not only enhance the immunity level stimulated by inactivated avian influenza virus (AIV) in vivo but also significantly inhibited the activity of activated AIV (H9N2 subtype) in vitro. G. filicina SP exhibited the strongest anti-AIV activity. These results revealed the variations in structural features and bioactivities among three SPs and indicated the potential adjuvants for immune-enhancement and anti-AIV. PMID:26729137

  17. Digital database of mining-related features at selected historic and active phosphate mines, Bannock, Bear Lake, Bingham, and Caribou counties, Idaho

    USGS Publications Warehouse

    Causey, J. Douglas; Moyle, Phillip R.

    2001-01-01

    This report provides a description of data and processes used to produce a spatial database that delineates mining-related features in areas of historic and active phosphate mining in the core of the southeastern Idaho phosphate resource area. The data have varying degrees of accuracy and attribution detail. Classification of areas by type of mining-related activity at active mines is generally detailed; however, the spatial coverage does not differentiate mining-related surface disturbance features at many of the closed or inactive mines. Nineteen phosphate mine sites are included in the study. A total of 5,728 hc (14,154 ac), or more than 57 km2 (22 mi2), of phosphate mining-related surface disturbance are documented in the spatial coverage of the core of the southeast Idaho phosphate resource area. The study includes 4 active phosphate mines—Dry Valley, Enoch Valley, Rasmussen Ridge, and Smoky Canyon—and 15 historic phosphate mines—Ballard, Champ, Conda, Diamond Gulch, Gay, Georgetown Canyon, Henry, Home Canyon, Lanes Creek, Maybe Canyon, Mountain Fuel, Trail Canyon, Rattlesnake Canyon, Waterloo, and Wooley Valley. Spatial data on the inactive historic mines is relatively up-to-date; however, spatially described areas for active mines are based on digital maps prepared in early 1999. The inactive Gay mine has the largest total area of disturbance: 1,917 hc (4,736 ac) or about 19 km2 (7.4 mi2). It encompasses over three times the disturbance area of the next largest mine, the Conda mine with 607 hc (1,504 ac), and it is nearly four times the area of the Smoky Canyon mine, the largest of the active mines with 497 hc (1,228 ac). The wide range of phosphate mining-related surface disturbance features (approximately 80) were reduced to 13 types or features used in this study—adit and pit, backfilled mine pit, facilities, mine pit, ore stockpile, railroad, road, sediment catchment, tailings or tailings pond, topsoil stockpile, water reservoir, and disturbed

  18. Electronic Structure and Multicatalytic Features of Redox-Active Bis(arylimino)acenaphthene (BIAN)-Derived Ruthenium Complexes.

    PubMed

    Singha Hazari, Arijit; Ray, Ritwika; Hoque, Md Asmaul; Lahiri, Goutam Kumar

    2016-08-15

    The article examines the newly designed and structurally characterized redox-active BIAN-derived [Ru(trpy)(R-BIAN)Cl]ClO4 ([1a]ClO4-[1c]ClO4), [Ru(trpy)(R-BIAN)(H2O)](ClO4)2 ([3a](ClO4)2-[3c](ClO4)2), and BIAO-derived [Ru(trpy)(BIAO)Cl]ClO4 ([2a]ClO4) (trpy = 2,2':6',2''-terpyridine, R-BIAN = bis(arylimino)acenaphthene (R = H (1a(+), 3a(2+)), 4-OMe (1b(+), 3b(2+)), 4-NO2 (1c(+), 3c(2+)), BIAO = [N-(phenyl)imino]acenapthenone). The experimental (X-ray, (1)H NMR, spectroelectrochemistry, EPR) and DFT/TD-DFT calculations of 1a(n)-1c(n) or 2a(n) collectively establish {Ru(II)-BIAN(0)} or {Ru(II)-BIAO(0)} configuration in the native state, metal-based oxidation to {Ru(III)-BIAN(0)} or {Ru(III)-BIAO(0)}, and successive electron uptake processes by the α-diimine fragment, followed by trpy and naphthalene π-system of BIAN or BIAO, respectively. The impact of the electron-withdrawing NO2 function in the BIAN moiety in 1c(+) has been reflected in the five nearby reduction steps within the accessible potential limit of -2 V versus SCE, leading to a fully reduced BIAN(4-) state in [1c](4-). The aqua derivatives ({Ru(II)-OH2}, 3a(2+)-3c(2+)) undergo simultaneous 2e(-)/2H(+) transfer to the corresponding {Ru(IV)═O} state and the catalytic current associated with the Ru(IV)/Ru(V) response probably implies its involvement in the electrocatalytic water oxidation. The aqua derivatives (3a(2+)-3c(2+)) are efficient and selective precatalysts in transforming a wide variety of alkenes to corresponding epoxides in the presence of PhI(OAc)2 as an oxidant in CH2Cl2 at 298 K as well as oxidation of primary, secondary, and heterocyclic alcohols with a large substrate scope with H2O2 as the stoichiometric oxidant in CH3CN at 343 K. The involvement of the {Ru(IV)═O} intermediate as the active catalyst in both the oxidation processes has been ascertained via a sequence of experimental evidence. PMID:27482834

  19. Features of Recently Transmitted HIV-1 Clade C Viruses that Impact Antibody Recognition: Implications for Active and Passive Immunization

    PubMed Central

    Rademeyer, Cecilia; Korber, Bette; Seaman, Michael S.; Giorgi, Elena E.; Thebus, Ruwayhida; Robles, Alexander; Sheward, Daniel J.; Wagh, Kshitij; Carey, Brittany R.; Gao, Hongmei; Greene, Kelli M.; Tang, Haili; Marais, Jinny C.; Diphoko, Thabo E.; Hraber, Peter; Tumba, Nancy; Moore, Penny L.; Gray, Glenda E.; Kublin, James; McElrath, M. Juliana; Vermeulen, Marion; Middelkoop, Keren; Bekker, Linda-Gail; Hoelscher, Michael; Maboko, Leonard; Makhema, Joseph; Robb, Merlin L.; Abdool Karim, Salim; Abdool Karim, Quarraisha; Kim, Jerome H.; Hahn, Beatrice H.; Gao, Feng; Swanstrom, Ronald; Morris, Lynn; Montefiori, David C.; Williamson, Carolyn

    2016-01-01

    The development of biomedical interventions to reduce acquisition of HIV-1 infection remains a global priority, however their potential effectiveness is challenged by very high HIV-1 envelope diversity. Two large prophylactic trials in high incidence, clade C epidemic regions in southern Africa are imminent; passive administration of the monoclonal antibody VRC01, and active immunization with a clade C modified RV144-like vaccines. We have created a large representative panel of C clade viruses to enable assessment of antibody responses to vaccines and natural infection in Southern Africa, and we investigated the genotypic and neutralization properties of recently transmitted clade C viruses to determine how viral diversity impacted antibody recognition. We further explore the implications of these findings for the potential effectiveness of these trials. A panel of 200 HIV-1 Envelope pseudoviruses was constructed from clade C viruses collected within the first 100 days following infection. Viruses collected pre-seroconversion were significantly more resistant to serum neutralization compared to post-seroconversion viruses (p = 0.001). Over 13 years of the study as the epidemic matured, HIV-1 diversified (p = 0.0009) and became more neutralization resistant to monoclonal antibodies VRC01, PG9 and 4E10. When tested at therapeutic levels (10ug/ml), VRC01 only neutralized 80% of viruses in the panel, although it did exhibit potent neutralization activity against sensitive viruses (IC50 titres of 0.42 μg/ml). The Gp120 amino acid similarity between the clade C panel and candidate C-clade vaccine protein boosts (Ce1086 and TV1) was 77%, which is 8% more distant than between CRF01_AE viruses and the RV144 CRF01_AE immunogen. Furthermore, two vaccine signature sites, K169 in V2 and I307 in V3, associated with reduced infection risk in RV144, occurred less frequently in clade C panel viruses than in CRF01_AE viruses from Thailand. Increased resistance of pre

  20. Features of Recently Transmitted HIV-1 Clade C Viruses that Impact Antibody Recognition: Implications for Active and Passive Immunization.

    PubMed

    Rademeyer, Cecilia; Korber, Bette; Seaman, Michael S; Giorgi, Elena E; Thebus, Ruwayhida; Robles, Alexander; Sheward, Daniel J; Wagh, Kshitij; Garrity, Jetta; Carey, Brittany R; Gao, Hongmei; Greene, Kelli M; Tang, Haili; Bandawe, Gama P; Marais, Jinny C; Diphoko, Thabo E; Hraber, Peter; Tumba, Nancy; Moore, Penny L; Gray, Glenda E; Kublin, James; McElrath, M Juliana; Vermeulen, Marion; Middelkoop, Keren; Bekker, Linda-Gail; Hoelscher, Michael; Maboko, Leonard; Makhema, Joseph; Robb, Merlin L; Abdool Karim, Salim; Abdool Karim, Quarraisha; Kim, Jerome H; Hahn, Beatrice H; Gao, Feng; Swanstrom, Ronald; Morris, Lynn; Montefiori, David C; Williamson, Carolyn

    2016-07-01

    The development of biomedical interventions to reduce acquisition of HIV-1 infection remains a global priority, however their potential effectiveness is challenged by very high HIV-1 envelope diversity. Two large prophylactic trials in high incidence, clade C epidemic regions in southern Africa are imminent; passive administration of the monoclonal antibody VRC01, and active immunization with a clade C modified RV144-like vaccines. We have created a large representative panel of C clade viruses to enable assessment of antibody responses to vaccines and natural infection in Southern Africa, and we investigated the genotypic and neutralization properties of recently transmitted clade C viruses to determine how viral diversity impacted antibody recognition. We further explore the implications of these findings for the potential effectiveness of these trials. A panel of 200 HIV-1 Envelope pseudoviruses was constructed from clade C viruses collected within the first 100 days following infection. Viruses collected pre-seroconversion were significantly more resistant to serum neutralization compared to post-seroconversion viruses (p = 0.001). Over 13 years of the study as the epidemic matured, HIV-1 diversified (p = 0.0009) and became more neutralization resistant to monoclonal antibodies VRC01, PG9 and 4E10. When tested at therapeutic levels (10ug/ml), VRC01 only neutralized 80% of viruses in the panel, although it did exhibit potent neutralization activity against sensitive viruses (IC50 titres of 0.42 μg/ml). The Gp120 amino acid similarity between the clade C panel and candidate C-clade vaccine protein boosts (Ce1086 and TV1) was 77%, which is 8% more distant than between CRF01_AE viruses and the RV144 CRF01_AE immunogen. Furthermore, two vaccine signature sites, K169 in V2 and I307 in V3, associated with reduced infection risk in RV144, occurred less frequently in clade C panel viruses than in CRF01_AE viruses from Thailand. Increased resistance of pre

  1. Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.

    PubMed

    Synytsya, Andriy; Choi, Doo Jin; Pohl, Radek; Na, Ye Seul; Capek, Peter; Lattová, Erika; Taubner, Tomáš; Choi, Ji Won; Lee, Chang Won; Park, Jae Kweon; Kim, Woo Jung; Kim, Sung Min; Lee, Jisun; Park, Yong Il

    2015-12-01

    Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010). However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount. These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains. The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged. The results of this

  2. 76 FR 7853 - Patient Safety Organizations: Voluntary Delisting From Oregon Patient Safety Commission

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-11

    ... HUMAN SERVICES Agency for Healthcare Research and Quality Patient Safety Organizations: Voluntary... Safety Organization (PSO). The Patient Safety and Quality Improvement Act of 2005 (Patient Safety Act... organizations whose mission and primary activity is to conduct activities to improve patient safety and...

  3. [Post-marketing clinical safety assessment of Shenmai injection based on active monitoring and passive monitoring in large data background].

    PubMed

    Wang, Lian-xin; Xie, Yan-ming; Ai, Qing-hua; Song, Nian-bin

    2015-12-01

    This paper adopted a series of related analysis methods to comprehensively analyze post-marketing clinical safety data of Shenmai injection from 4,220 cases of SRS and 32,358 cases of multicenter, prospective, registered hospital centralized monitoring in large data background, calculated ADR incidence rate was 0.93 per 1,000, main symptoms of ADR includes chest pain, chills, skin itching, palpitations, fever, nausea, dizziness, vomiting, flushing, numbness, allergic reaction, cyanosis, rash, low back pain, and "breath", "anaphylactoid reaction" and "flush" were the safety warning signals of Shenmai injection. Primary disease for chronic pulmonary heart disease, thyroid disease, and combined with cerebral vascular disease, prior to the injection and continuous use of alprostadil, cyclic adenosine monophosphate, combined with quinolones, penicillins were suspicious influence factors of ADR of Shenmai injection, these promot the clinical safety. PMID:27245017

  4. Sustainable development and next generation's health: a long-term perspective about the consequences of today's activities for food safety.

    PubMed

    Frazzoli, Chiara; Petrini, Carlo; Mantovani, Alberto

    2009-01-01

    Development is defined sustainable when it meets the needs of the present without compromising the ability of future generations to meet their own needs. Pivoting on social, environmental and economic aspects of food chain sustainability, this paper presents the concept of sustainable food safety based on the prevention of risks and burden of poor health for generations to come. Under this respect, the assessment of long-term, transgenerational risks is still hampered by serious scientific uncertainties. Critical issues to the development of a sustainable food safety framework may include: endocrine disrupters as emerging contaminants that specifically target developing organisms; toxicological risks assessment in Countries at the turning point of development; translating knowledge into toxicity indexes to support risk management approaches, such as hazard analysis and critical control points (HACCP); the interplay between chemical hazards and social determinants. Efforts towards the comprehensive knowledge and management of key factors of sustainable food safety appear critical to the effectiveness of the overall sustainability policies.

  5. Associations between Moderate-to-Vigorous Physical Activity and Neighbourhood Recreational Facilities: The Features of the Facilities Matter

    PubMed Central

    Lee, Ka Yiu; Lee, Paul H.; Macfarlane, Duncan

    2014-01-01

    Objectives: To examine the associations between objectively-assessed moderate-to-vigorous physical activity (MVPA) and perceived/objective measures of neighbourhood recreational facilities categorized into indoor or outdoor, public, residential or commercial facilities. The associations between facility perceptions and objectively-assessed numbers of recreational facilities were also examined. Method: A questionnaire was used on 480 adults to measure local facility perceptions, with 154 participants wearing ActiGraph accelerometers for ≥4 days. The objectively-assessed number of neighbourhood recreational facilities were examined using direct observations and Geographical Information System data. Results: Both positive and negative associations were found between MVPA and perceived/objective measures of recreational facilities. Some associations depended on whether the recreational facilities were indoor or outdoor, public or residential facilities. The objectively-assessed number of most public recreational facilities was associated with the corresponding facility perceptions, but the size of effect was generally lower than for residential recreational facilities. Conclusions: The objectively-assessed number of residential outdoor table tennis courts and public indoor swimming pools, the objectively-assessed presence of tennis courts and swimming pools, and the perceived presence of bike lanes and swimming pools were positive determinants of MVPA. It is suggested to categorize the recreational facilities into smaller divisions in order to identify unique associations with MVPA. PMID:25485980

  6. A Detailed Data-Driven Network Model of Prefrontal Cortex Reproduces Key Features of In Vivo Activity

    PubMed Central

    Hass, Joachim; Hertäg, Loreen; Durstewitz, Daniel

    2016-01-01

    The prefrontal cortex is centrally involved in a wide range of cognitive functions and their impairment in psychiatric disorders. Yet, the computational principles that govern the dynamics of prefrontal neural networks, and link their physiological, biochemical and anatomical properties to cognitive functions, are not well understood. Computational models can help to bridge the gap between these different levels of description, provided they are sufficiently constrained by experimental data and capable of predicting key properties of the intact cortex. Here, we present a detailed network model of the prefrontal cortex, based on a simple computationally efficient single neuron model (simpAdEx), with all parameters derived from in vitro electrophysiological and anatomical data. Without additional tuning, this model could be shown to quantitatively reproduce a wide range of measures from in vivo electrophysiological recordings, to a degree where simulated and experimentally observed activities were statistically indistinguishable. These measures include spike train statistics, membrane potential fluctuations, local field potentials, and the transmission of transient stimulus information across layers. We further demonstrate that model predictions are robust against moderate changes in key parameters, and that synaptic heterogeneity is a crucial ingredient to the quantitative reproduction of in vivo-like electrophysiological behavior. Thus, we have produced a physiologically highly valid, in a quantitative sense, yet computationally efficient PFC network model, which helped to identify key properties underlying spike time dynamics as observed in vivo, and can be harvested for in-depth investigation of the links between physiology and cognition. PMID:27203563

  7. Common and distinct features of mammary tumors driven by Pten-deletion or activating Pik3ca mutation

    PubMed Central

    Liu, Jeff C.; Wang, Dong-Yu; Egan, Sean E.; Zacksenhaus, Eldad

    2016-01-01

    PTEN loss and PIK3CA activation both promote the accumulation of phosphatidylinositol (3, 4, 5)-trisphosphate (PIP3). While these proteins also have distinct biochemical functions, beyond the regulation of PIP3, little is known about the consequences of these differences in vivo. Here, we directly compared cancer signalling in mammary tumors from MMTV-Cre:Ptenf/f and MMTV-Cre:Pik3caLSL-H1047R mice. Using unsupervised hierarchical clustering we found that whereas MMTV-Cre:Pik3caLSL-H1047R-derived tumors fall into two separate groups, designated squamous-likeEx and class14Ex, MMTV-Cre:Ptenf/f tumors cluster as one group together with PIK3CAH1047R class14Ex, exhibiting a ‘luminal’ expression profile. Gene Set Enrichment Analysis (GSEA) of PtenΔ and PIK3CAH1047R class14Ex tumors revealed very similar profiles of signalling pathways as well as some interesting differences. Analysis of 18 signalling signatures revealed that PI3K signalling is significantly induced whereas EGFR signalling is significantly reduced in PtenΔ versus PIK3CAH1047R tumors. Thus, PtenΔ and PIK3CAH1047R tumors exhibit discernable differences that may impact tumorigenesis and response to therapy. PMID:26814435

  8. Common fragile sites are conserved features of human and mouse chromosomes and relate to large active genes

    PubMed Central

    Helmrich, Anne; Stout-Weider, Karen; Hermann, Klaus; Schrock, Evelin; Heiden, Thomas

    2006-01-01

    Common fragile sites (CFSs) are seen as chromosomal gaps and breaks brought about by inhibition of replication, and it is thought that they cluster with tumor breakpoints. This study presents a comprehensive analysis using conventional and molecular cytogenetic mapping of CFSs and their expression frequencies in two mouse strains, BALB/c and C57BL/6, and in human probands. Here we show that induced mouse CFSs relate to sites of spontaneous gaps and breaks and that CFS expression levels in chromosome bands are conserved between the two mouse strains and between syntenic mouse and human DNA segments. Furthermore, four additional mouse CFSs were found to be homologous to human CFSs on the molecular cytogenetic level (Fra2D-FRA2G, Fra4C2-FRA9E, Fra6A3.1-FRA7G, and Fra6B1-FRA7H), increasing the number of such CFSs already described in the literature to eight. Contrary to previous reports, DNA helix flexibility is not increased in the 15 human and eight mouse CFSs molecularly defined so far, compared to large nonfragile control regions. Our findings suggest that the mechanisms that provoke instability at CFSs are evolutionarily conserved. The role that large transcriptionally active genes may play in CFS expression is discussed. PMID:16954539

  9. WALK 2.0: Examining the effectiveness of Web 2.0 features to increase physical activity in a ‘real world’ setting: an ecological trial

    PubMed Central

    Caperchione, Cristina M; Kolt, Gregory S; Savage, Trevor N; Rosenkranz, Richard R; Maeder, Anthony J; Vandelanotte, Corneel; Duncan, Mitch J; Van Itallie, Anetta; Tague, Rhys; Mummery, W Kerry

    2014-01-01

    Introduction Low levels of health-enhancing physical activity require novel approaches that have the potential to reach broad populations. Web-based interventions are a popular approach for behaviour change given their wide reach and accessibility. However, challenges with participant engagement and retention reduce the long-term maintenance of behaviour change. Web 2.0 features present a new and innovative online environment supporting greater interactivity, with the potential to increase engagement and retention. In order to understand the applicability of these innovative interventions for the broader population, ‘real-world’ interventions implemented under ‘everyday conditions’ are required. The aim of this study is to investigate the difference in physical activity behaviour between individuals using a traditional Web 1.0 website with those using a novel Web 2.0 website. Methods and analysis In this study we will aim to recruit 2894 participants. Participants will be recruited from individuals who register with a pre-existing health promotion website that currently provides Web 1.0 features (http://www.10000steps.org.au). Eligible participants who provide informed consent will be randomly assigned to one of the two trial conditions: the pre-existing 10 000 Steps website (with Web 1.0 features) or the newly developed WALK 2.0 website (with Web 2.0 features). Primary and secondary outcome measures will be assessed by self-report at baseline, 3 months and 12 months, and include: physical activity behaviour, height and weight, Internet self-efficacy, website usability, website usage and quality of life. Ethics and dissemination This study has received ethics approval from the University of Western Sydney Human Research Ethics Committee (Reference Number H8767) and has been funded by the National Health and Medical Research Council (Reference Number 589903). Study findings will be disseminated widely through peer-reviewed publications, academic

  10. Lead users’ ideas on core features to support physical activity in rheumatoid arthritis: a first step in the development of an internet service using participatory design

    PubMed Central

    2014-01-01

    Background Despite the growing evidence of the benefits of physical activity (PA) in individuals with rheumatoid arthritis (RA), the majority is not physically active enough. An innovative strategy is to engage lead users in the development of PA interventions provided over the internet. The aim was to explore lead users’ ideas and prioritization of core features in a future internet service targeting adoption and maintenance of healthy PA in people with RA. Methods Six focus group interviews were performed with a purposively selected sample of 26 individuals with RA. Data were analyzed with qualitative content analysis and quantification of participants’ prioritization of most important content. Results Six categories were identified as core features for a future internet service: up-to-date and evidence-based information and instructions, self-regulation tools, social interaction, personalized set-up, attractive design and content, and access to the internet service. The categories represented four themes, or core aspects, important to consider in the design of the future service: (1) content, (2) customized options, (3) user interface and (4) access and implementation. Conclusions This is, to the best of our knowledge, the first study involving people with RA in the development of an internet service to support the adoption and maintenance of PA. Participants helped identifying core features and aspects important to consider and further explore during the next phase of development. We hypothesize that involvement of lead users will make transfer from theory to service more adequate and user-friendly and therefore will be an effective mean to facilitate PA behavior change. PMID:24655757

  11. Current concepts on integrative safety assessment of active substances of botanical, mineral or chemical origin in homeopathic medicinal products within the European regulatory framework.

    PubMed

    Buchholzer, Marie-Luise; Werner, Christine; Knoess, Werner

    2014-03-01

    For active substances of botanical, mineral or chemical origin processed in homeopathic medicinal products for human use, the adequate safety principles as with other human medicinal products are applied in line with the European regulatory framework. In homeopathy, nonclinical safety assessment is facing a particular challenge because of a multitude and diversity of source materials used and due to rarely available toxicological data. Thus, current concepts applied by the national regulatory authority in Germany (BfArM) on integrative safety assessment of raw materials used in homeopathic medicinal products involve several evaluation approaches like the use of the Lowest Human Recommended Dose (LHRD), toxicological limit values, Threshold of Toxicological Concern (TTC), data from food regulation or the consideration of unavoidable environmental or dietary background exposure. This publication is intended to further develop and clarify the practical use of these assessment routes by exemplary application on selected homeopathic preparations. In conclusion, the different approaches are considered a very useful scientific and simultaneously pragmatic procedure in differentiated risk assessment of homeopathic medicinal products. Overall, this paper aims to increase the visibility of the safety issues in homeopathy and to stimulate scientific discussion of worldwide existing regulatory concepts on homeopathic medicinal products.

  12. The 2006-2007 Active Phase Of Anomalous X-Ray Pulsar 4U 0142+61: Radiative and Timing Changes, Bursts, and Burst Spectral Features

    NASA Technical Reports Server (NTRS)

    Gavril, Fotis P.; Dib, Rim; Kaspi, Victoria M.

    2009-01-01

    After at least 6 years of quiescence, Anomalous X-ray Pulsar (AXP) 4U 0142+61 entered an active phase in 2006 March that lasted several months and included six X-ray bursts as well as many changes in the persistent X-ray emission. The bursts, the first seen from this AXP in >11 years of Rossi X-ray Timing Explorer monitoring, all occurred in the interval between 2006 April 6 and 2007 February 7. The burst durations ranged from 8-3x10(exp 3)s. The first five burst spectra are well modeled by blackbodies, with temperatures kT approx. 2 - 6 keV. However, the sixth burst had a complicated spectrum that is well characterized by a blackbody plus three emission features whose amplitude varied throughout the burst. The most prominent feature was at 14.0 keV. Upon entry into the active phase the pulsar showed a significant change in pulse morphology and a likely timing glitch. The glitch had a total frequency jump of (1.9+/-0.4)x10(exp -7) Hz, which recovered with a decay time of 17+/-2 days by more than the initial jump, implying a net spin-down of the pulsar. We discuss these events in the context of the magnetar model.

  13. General features

    SciTech Connect

    Wallace, R.E.

    1990-01-01

    The San Andreas fault system, a complex of faults that display predominantly large-scale strike slip, is part of an even more complex system of faults, isolated segments of the East Pacific Rise, and scraps of plates lying east of the East Pacific Rise that collectively separate the North American plate from the Pacific plate. This chapter briefly describes the San Andreas fault system, its setting along the Pacific Ocean margin of North America, its extent, and the patterns of faulting. Only selected characteristics are described, and many features are left for depictions on maps and figures.

  14. FLUOR HANFORD SAFETY MANAGEMENT PROGRAMS

    SciTech Connect

    GARVIN, L. J.; JENSEN, M. A.

    2004-04-13

    This document summarizes safety management programs used within the scope of the ''Project Hanford Management Contract''. The document has been developed to meet the format and content requirements of DOE-STD-3009-94, ''Preparation Guide for US. Department of Energy Nonreactor Nuclear Facility Documented Safety Analyses''. This document provides summary descriptions of Fluor Hanford safety management programs, which Fluor Hanford nuclear facilities may reference and incorporate into their safety basis when producing facility- or activity-specific documented safety analyses (DSA). Facility- or activity-specific DSAs will identify any variances to the safety management programs described in this document and any specific attributes of these safety management programs that are important for controlling potentially hazardous conditions. In addition, facility- or activity-specific DSAs may identify unique additions to the safety management programs that are needed to control potentially hazardous conditions.

  15. Airline Safety and Economy

    NASA Technical Reports Server (NTRS)

    1993-01-01

    This video documents efforts at NASA Langley Research Center to improve safety and economy in aircraft. Featured are the cockpit weather information needs computer system, which relays real time weather information to the pilot, and efforts to improve techniques to detect structural flaws and corrosion, such as the thermal bond inspection system.

  16. EDITORIAL: Safety aspects of fusion power plants

    NASA Astrophysics Data System (ADS)

    Kolbasov, B. N.

    2007-07-01

    importance for the fusion power plant research programmes. The objective of this Technical Meeting was to examine in an integrated way all the safety aspects anticipated to be relevant to the first fusion power plant prototype expected to become operational by the middle of the century, leading to the first generation of economically viable fusion power plants with attractive S&E features. After screening by guest editors and consideration by referees, 13 (out of 28) papers were accepted for publication. They are devoted to the following safety topics: power plant safety; fusion specific operational safety approaches; test blanket modules; accident analysis; tritium safety and inventories; decommissioning and waste. The paper `Main safety issues at the transition from ITER to fusion power plants' by W. Gulden et al (EU) highlights the differences between ITER and future fusion power plants with magnetic confinement (off-site dose acceptance criteria, consequences of accidents inside and outside the design basis, occupational radiation exposure, and waste management, including recycling and/or final disposal in repositories) on the basis of the most recent European fusion power plant conceptual study. Ongoing S&E studies within the US inertial fusion energy (IFE) community are focusing on two design concepts. These are the high average power laser (HAPL) programme for development of a dry-wall, laser-driven IFE power plant, and the Z-pinch IFE programme for the production of an economically-attractive power plant using high-yield Z-pinch-driven targets. The main safety issues related to these programmes are reviewed in the paper `Status of IFE safety and environmental activities in the US' by S. Reyes et al (USA). The authors propose future directions of research in the IFE S&E area. In the paper `Recent accomplishments and future directions in the US Fusion Safety & Environmental Program' D. Petti et al (USA) state that the US fusion programme has long recognized that the S

  17. Focusing on dam safety

    SciTech Connect

    Lagassa, G.

    1993-01-01

    With increased relicensing activity and a federal emphasis on safety, dam repair and refurbishment is a growing business. Providers of goods and services are gearing up to meet the dam repair and rehabilitation needs that result.

  18. Activated human mesenchymal stem/stromal cells suppress metastatic features of MDA-MB-231 cells by secreting IFN-β.

    PubMed

    Yoon, N; Park, M S; Shigemoto, T; Peltier, G; Lee, R H

    2016-01-01

    Our recent study showed that human mesenchymal stem/stromal cells (hMSCs) are activated to express tumor necrosis factor (TNF)-α-related apoptosis-inducing ligand (TRAIL) by exposure to TNF-α and these activated hMSCs effectively induce apoptosis in triple-negative breast cancer MDA-MB-231 (MDA) cells in vitro and in vivo. Here, we further demonstrated that activated hMSCs not only induced apoptosis of MDA cells but also reduced metastatic features in MDA cells. These activated hMSC-exposed MDA cells showed reduced tumorigenicity and suppressed formation of lung metastasis when implanted in the mammary fat pad. Surprisingly, the activated hMSC-exposed MDA cells increased TRAIL expression, resulting in apoptosis in MDA cells. Interestingly, upregulation of TRAIL in MDA cells was mediated by interferon-beta (IFN-β) secreted from activated hMSCs. Furthermore, IFN-β in activated hMSCs was induced by RNA and DNA released from apoptotic MDA cells in absent in melanoma 2 (AIM2) and IFN induced with helicase C domain 1 (IFIH1)-dependent manners. These observations were only seen in the TRAIL-sensitive breast cancer cell lines but not in the TRAIL-resistant breast cancer cell lines. Consistent with these results, Kaplan-Meier survival analysis also showed that lack of innate sensors detecting DNA or RNA is strongly associated with poor survival in estrogen receptor-negative breast cancer patients. In addition, cancer-associated fibroblasts (CAF) isolated from a breast cancer patient were also able to express TRAIL and IFN-β upon DNA and RNA stimulation. Therefore, our results suggest that the crosstalk between TRAIL-sensitive cancer cells and stromal cells creates a tumor-suppressive microenvironment and further provide a novel therapeutic approach to target stromal cells within cancer microenvironment for TRAIL sensitive cancer treatment. PMID:27077807

  19. Activated human mesenchymal stem/stromal cells suppress metastatic features of MDA-MB-231 cells by secreting IFN-β

    PubMed Central

    Yoon, N; Park, M S; Shigemoto, T; Peltier, G; Lee, R H

    2016-01-01

    Our recent study showed that human mesenchymal stem/stromal cells (hMSCs) are activated to express tumor necrosis factor (TNF)-α-related apoptosis-inducing ligand (TRAIL) by exposure to TNF-α and these activated hMSCs effectively induce apoptosis in triple-negative breast cancer MDA-MB-231 (MDA) cells in vitro and in vivo. Here, we further demonstrated that activated hMSCs not only induced apoptosis of MDA cells but also reduced metastatic features in MDA cells. These activated hMSC-exposed MDA cells showed reduced tumorigenicity and suppressed formation of lung metastasis when implanted in the mammary fat pad. Surprisingly, the activated hMSC-exposed MDA cells increased TRAIL expression, resulting in apoptosis in MDA cells. Interestingly, upregulation of TRAIL in MDA cells was mediated by interferon-beta (IFN-β) secreted from activated hMSCs. Furthermore, IFN-β in activated hMSCs was induced by RNA and DNA released from apoptotic MDA cells in absent in melanoma 2 (AIM2) and IFN induced with helicase C domain 1 (IFIH1)-dependent manners. These observations were only seen in the TRAIL-sensitive breast cancer cell lines but not in the TRAIL-resistant breast cancer cell lines. Consistent with these results, Kaplan–Meier survival analysis also showed that lack of innate sensors detecting DNA or RNA is strongly associated with poor survival in estrogen receptor-negative breast cancer patients. In addition, cancer-associated fibroblasts (CAF) isolated from a breast cancer patient were also able to express TRAIL and IFN-β upon DNA and RNA stimulation. Therefore, our results suggest that the crosstalk between TRAIL-sensitive cancer cells and stromal cells creates a tumor-suppressive microenvironment and further provide a novel therapeutic approach to target stromal cells within cancer microenvironment for TRAIL sensitive cancer treatment. PMID:27077807

  20. 76 FR 14980 - BOEMRE Information Collection Activity: 1010-0185, Increased Safety Measures for Oil and Gas...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-18

    ... cause damage to the environment or to property or endanger life or health.'' The interim rule (75 FR... process, on October 14, 2010, we published a Federal Register notice (75 FR 63346) associated with the IFR...: 1010-0185, Increased Safety Measures for Oil and Gas Drilling, Well-Completion, and Well-...