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Sample records for active zone az

  1. 78 FR 19988 - Safety Zone; BWRC Spring Classic, Parker, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-03

    ... Security FR Federal Register NPRM Notice of Proposed Rulemaking A. Regulatory History and Information The... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; BWRC Spring Classic, Parker, AZ AGENCY..., Arizona for the Blue Water Resort and Casino Spring Classic. This temporary safety zone is necessary...

  2. 75 FR 19250 - Safety Zone; BWRC Spring Classic, Parker, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; BWRC Spring Classic, Parker, AZ AGENCY..., Arizona for the Blue Water Resort and Casino Spring Classic. This temporary safety zone is necessary to... Speedboat Club is sponsoring the Blue Water Resort and Casino Spring Classic, which is held on the...

  3. Synaptic Vesicle Proteins and Active Zone Plasticity

    PubMed Central

    Kittel, Robert J.; Heckmann, Manfred

    2016-01-01

    Neurotransmitter is released from synaptic vesicles at the highly specialized presynaptic active zone (AZ). The complex molecular architecture of AZs mediates the speed, precision and plasticity of synaptic transmission. Importantly, structural and functional properties of AZs vary significantly, even for a given connection. Thus, there appear to be distinct AZ states, which fundamentally influence neuronal communication by controlling the positioning and release of synaptic vesicles. Vice versa, recent evidence has revealed that synaptic vesicle components also modulate organizational states of the AZ. The protein-rich cytomatrix at the active zone (CAZ) provides a structural platform for molecular interactions guiding vesicle exocytosis. Studies in Drosophila have now demonstrated that the vesicle proteins Synaptotagmin-1 (Syt1) and Rab3 also regulate glutamate release by shaping differentiation of the CAZ ultrastructure. We review these unexpected findings and discuss mechanistic interpretations of the reciprocal relationship between synaptic vesicles and AZ states, which has heretofore received little attention. PMID:27148040

  4. 76 FR 30907 - Proposed Foreign-Trade Zone-Eloy, AZ; Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... Foreign-Trade Zones Board Proposed Foreign-Trade Zone--Eloy, AZ; Application An application has been submitted to the Foreign-Trade Zones Board (the Board) by the City of Eloy to establish a general-purpose foreign- trade zone at sites in Pinal County, Arizona, adjacent to the Phoenix U.S. Customs and...

  5. Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene.

    PubMed

    Cheng, Chunzhen; Zhang, Lingyun; Yang, Xuelian; Zhong, Guangyan

    2015-10-01

    On-tree storage and harvesting of mature fruit account for a large proportion of cost in the production of citrus, and a reduction of the cost would not be achieved without a thorough understanding of the mechani sm of the mature fruit abscission. Genome-wide gene expression changes in ethylene-treated fruit calyx abscission zone (AZ-C) of Citrus sinensis cv. Olinda were therefore investigated using a citrus genome array representing up to 33,879 citrus transcripts. In total, 1313 and 1044 differentially regulated genes were identified in AZ-C treated with ethylene for 4 and 24 h, respectively. The results showed that mature citrus fruit abscission commenced with the activation of ethylene signal transduction pathway that led to the activation of ethylene responsive transcription factors and the subsequent transcriptional regulation of a large set of ethylene responsive genes. Significantly down-regulated genes included those of starch/sugar biosynthesis, transportation of water and growth promoting hormone synthesis and signaling, whereas significantly up-regulated genes were those involved in defense, cell wall degradation, and secondary metabolism. Our data unraveled the underlying mechanisms of some known important biochemical events occurring at AZ-C and should provide informative suggestions for future manipulation of the events to achieve a controllable abscission for mature citrus fruit. PMID:25948248

  6. 75 FR 20920 - Safety Zone; Lake Havasu Grand Prix, Lake Havasu, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-22

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Lake Havasu Grand Prix, Lake Havasu, AZ... temporary safety zone upon the navigable waters of Lake Havasu on the Colorado River in Lake Havasu City, Arizona for the Lake Havasu Grand Prix. This temporary safety zone is necessary to provide for the...

  7. 77 FR 62216 - Proposed Foreign-Trade Zone-Eloy, AZ Comment Period on New Evidence

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-12

    ..., requesting authority to establish a general-purpose FTZ at sites in Pinal County, Arizona (76 FR 30907, 05/27... Foreign-Trade Zones Board Proposed Foreign-Trade Zone--Eloy, AZ Comment Period on New Evidence On May 23... 15 CFR 400.27(d)(2)(v)(B)), the FTZ Board is now inviting public comment on new evidence submitted...

  8. 78 FR 17097 - Safety Zone; Lake Havasu Triathlon; Lake Havasu City, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-20

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Lake Havasu Triathlon; Lake Havasu City, AZ... temporary safety zone within the navigable waters of Lake Havasu and the London Bridge Channel for the Lake... Triathlon will consist of 600 participants. The waterside swim course consists of 1500 meters in Lake...

  9. 78 FR 66267 - Safety Zone; HITS Triathlon Series; Colorado River; Lake Havasu, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; HITS Triathlon Series; Colorado River; Lake... Bay in Lake Havasu, AZ. The safety zone will encompass the waters in the northern portion of...

  10. 75 FR 22697 - Safety Zone; APBA National Tour, Parker, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... safety zone within the Lake Moolvalya region of the navigable waters of the Colorado River in Parker... representative. The limits of this temporary safety zone are the portion of the Colorado River from Headgate Dam... anchor in a portion of the Colorado River from 6 a.m. to 6 p.m. on April 30, 2010 through May 2,...

  11. 78 FR 60698 - Safety Zone, Lucas Oil Drag Boat Racing Series; Thompson Bay, Lake Havasu City, AZ.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone, Lucas Oil Drag Boat Racing Series; Thompson..., AZ for the Lucas Oil Drag Boat Racing Series. This temporary safety zone is necessary to...

  12. Super-resolution microscopy of the synaptic active zone

    PubMed Central

    Ehmann, Nadine; Sauer, Markus; Kittel, Robert J.

    2015-01-01

    Brain function relies on accurate information transfer at chemical synapses. At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Calcium channels are pivotal for the initiation of excitation-secretion coupling and, correspondingly, capture a central position at the AZ. Combining quantitative functional studies with modeling approaches has provided predictions of channel properties, numbers and even positions on the nanometer scale. However, elucidating the nanoscopic organization of the surrounding protein network requires direct ultrastructural access. Without this information, knowledge of molecular synaptic structure-function relationships remains incomplete. Recently, super-resolution microscopy (SRM) techniques have begun to enter the neurosciences. These approaches combine high spatial resolution with the molecular specificity of fluorescence microscopy. Here, we discuss how SRM can be used to obtain information on the organization of AZ proteins. PMID:25688186

  13. Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states

    NASA Astrophysics Data System (ADS)

    Ehmann, Nadine; van de Linde, Sebastian; Alon, Amit; Ljaschenko, Dmitrij; Keung, Xi Zhen; Holm, Thorge; Rings, Annika; Diantonio, Aaron; Hallermann, Stefan; Ashery, Uri; Heckmann, Manfred; Sauer, Markus; Kittel, Robert J.

    2014-08-01

    The precise molecular architecture of synaptic active zones (AZs) gives rise to different structural and functional AZ states that fundamentally shape chemical neurotransmission. However, elucidating the nanoscopic protein arrangement at AZs is impeded by the diffraction-limited resolution of conventional light microscopy. Here we introduce new approaches to quantify endogenous protein organization at single-molecule resolution in situ with super-resolution imaging by direct stochastic optical reconstruction microscopy (dSTORM). Focusing on the Drosophila neuromuscular junction (NMJ), we find that the AZ cytomatrix (CAZ) is composed of units containing ~137 Bruchpilot (Brp) proteins, three quarters of which are organized into about 15 heptameric clusters. We test for a quantitative relationship between CAZ ultrastructure and neurotransmitter release properties by engaging Drosophila mutants and electrophysiology. Our results indicate that the precise nanoscopic organization of Brp distinguishes different physiological AZ states and link functional diversification to a heretofore unrecognized neuronal gradient of the CAZ ultrastructure.

  14. 77 FR 20356 - Foreign-Trade Zone 277-Western Maricopa County, AZ; Application for Manufacturing Authority...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-04

    ... Manufacturing Authority; Suntech Arizona, Inc., (Solar Panel Manufacturing), Goodyear, AZ An application has... 275 and 290 watt solar panels for industrial use. Components and materials sourced from abroad... to choose the duty rates during customs entry procedures that apply to solar panels (duty-free)...

  15. Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila.

    PubMed

    Wagh, Dhananjay A; Rasse, Tobias M; Asan, Esther; Hofbauer, Alois; Schwenkert, Isabell; Dürrbeck, Heike; Buchner, Sigrid; Dabauvalle, Marie-Christine; Schmidt, Manuela; Qin, Gang; Wichmann, Carolin; Kittel, Robert; Sigrist, Stephan J; Buchner, Erich

    2006-03-16

    Neurotransmitters are released at presynaptic active zones (AZs). In the fly Drosophila, monoclonal antibody (MAB) nc82 specifically labels AZs. We employ nc82 to identify Bruchpilot protein (BRP) as a previously unknown AZ component. BRP shows homology to human AZ protein ELKS/CAST/ERC, which binds RIM1 in a complex with Bassoon and Munc13-1. The C terminus of BRP displays structural similarities to multifunctional cytoskeletal proteins. During development, transcription of the bruchpilot locus (brp) coincides with neuronal differentiation. Panneural reduction of BRP expression by RNAi constructs permits a first functional characterization of this large AZ protein: larvae show reduced evoked but normal spontaneous transmission at neuromuscular junctions. In adults, we observe loss of T bars at active zones, absence of synaptic components in electroretinogram, locomotor inactivity, and unstable flight (hence "bruchpilot"-crash pilot). We propose that BRP is critical for intact AZ structure and normal-evoked neurotransmitter release at chemical synapses of Drosophila. PMID:16543132

  16. Effect of Friction Stir Processing on Microstructure and Mechanical Properties of AZ91C Magnesium Cast Alloy Weld Zone

    NASA Astrophysics Data System (ADS)

    Hassani, Behzad; Karimzadeh, Fathallah; Enayati, Mohammad Hossein; Sabooni, Soheil; Vallant, Rudolf

    2016-07-01

    In this study, friction stir processing (FSP) was applied to the GTAW (TIG)-welded AZ91C cast alloy to refine the microstructure and optimize the mechanical properties of the weld zone. Microstructural investigation of the samples was performed by optical microscopy and the phases in the microstructure were determined by x-ray diffraction (XRD). The microstructural evaluations showed that FSP destroys the coarse dendritic microstructure. Furthermore, it dissolves the secondary hard and brittle β-Mg17Al12 phase existing at grain boundaries of the TIG weld zone. The closure and decrease in amount of porosities along with the elimination of the cracks in the microstructure were observed. These changes were followed by a significant grain refinement to an average value of 11 µm. The results showed that the hardness values increased to the mean ones, respectively, for as-cast (63 Hv), TIG weld zone (67 Hv), and stir zone (79 Hv). The yield and ultimate strength were significantly enhanced after FSP. The fractography evaluations, by scanning electron microscopy (SEM), indicated to a transition from brittle to ductile fracture surface after applying FSP to the TIG weld zone.

  17. Effect of Friction Stir Processing on Microstructure and Mechanical Properties of AZ91C Magnesium Cast Alloy Weld Zone

    NASA Astrophysics Data System (ADS)

    Hassani, Behzad; Karimzadeh, Fathallah; Enayati, Mohammad Hossein; Sabooni, Soheil; Vallant, Rudolf

    2016-05-01

    In this study, friction stir processing (FSP) was applied to the GTAW (TIG)-welded AZ91C cast alloy to refine the microstructure and optimize the mechanical properties of the weld zone. Microstructural investigation of the samples was performed by optical microscopy and the phases in the microstructure were determined by x-ray diffraction (XRD). The microstructural evaluations showed that FSP destroys the coarse dendritic microstructure. Furthermore, it dissolves the secondary hard and brittle β-Mg17Al12 phase existing at grain boundaries of the TIG weld zone. The closure and decrease in amount of porosities along with the elimination of the cracks in the microstructure were observed. These changes were followed by a significant grain refinement to an average value of 11 µm. The results showed that the hardness values increased to the mean ones, respectively, for as-cast (63 Hv), TIG weld zone (67 Hv), and stir zone (79 Hv). The yield and ultimate strength were significantly enhanced after FSP. The fractography evaluations, by scanning electron microscopy (SEM), indicated to a transition from brittle to ductile fracture surface after applying FSP to the TIG weld zone.

  18. H2A.Z has a function reminiscent of an activator required for preferential binding to intergenic DNA

    PubMed Central

    Larochelle, Marc; Gaudreau, Luc

    2003-01-01

    H2A.Z has been shown to regulate transcription in yeast, and that function resides in its C-terminal region as the reciprocal portion of H2A cannot substitute for the latter. We show that fusion of a transcriptional activating region to the C-terminal region of H2A, which is substituted for that of H2A.Z, can allow the chimera to fulfil the special role of H2A.Z in positive gene regulation, as well as complement growth deficiencies of htz1Δ cells. We further show that the ‘transcription’ function of H2A.Z is linked to its ability to preferentially localize to certain intergenic DNA regions. Our results suggest that H2A.Z modulates functional interactions with transcription regulatory components, and thus increases its localization to promoters where it helps poise chromatin for gene activation. PMID:12941702

  19. RIM-binding protein, a central part of the active zone, is essential for neurotransmitter release.

    PubMed

    Liu, Karen S Y; Siebert, Matthias; Mertel, Sara; Knoche, Elena; Wegener, Stephanie; Wichmann, Carolin; Matkovic, Tanja; Muhammad, Karzan; Depner, Harald; Mettke, Christoph; Bückers, Johanna; Hell, Stefan W; Müller, Martin; Davis, Graeme W; Schmitz, Dietmar; Sigrist, Stephan J

    2011-12-16

    The molecular machinery mediating the fusion of synaptic vesicles (SVs) at presynaptic active zone (AZ) membranes has been studied in detail, and several essential components have been identified. AZ-associated protein scaffolds are viewed as only modulatory for transmission. We discovered that Drosophila Rab3-interacting molecule (RIM)-binding protein (DRBP) is essential not only for the integrity of the AZ scaffold but also for exocytotic neurotransmitter release. Two-color stimulated emission depletion microscopy showed that DRBP surrounds the central Ca(2+) channel field. In drbp mutants, Ca(2+) channel clustering and Ca(2+) influx were impaired, and synaptic release probability was drastically reduced. Our data identify RBP family proteins as prime effectors of the AZ scaffold that are essential for the coupling of SVs, Ca(2+) channels, and the SV fusion machinery. PMID:22174254

  20. Respiratory Syncytial Virus Inhibitor AZ-27 Differentially Inhibits Different Polymerase Activities at the Promoter

    PubMed Central

    Noton, Sarah L.; Nagendra, Kartikeya; Dunn, Ewan F.; Mawhorter, Michael E.; Yu, Qin

    2015-01-01

    ABSTRACT Respiratory syncytial virus (RSV) is the leading cause of pediatric respiratory disease. RSV has an RNA-dependent RNA polymerase that transcribes and replicates the viral negative-sense RNA genome. The large polymerase subunit (L) has multiple enzymatic activities, having the capability to synthesize RNA and add and methylate a cap on each of the viral mRNAs. Previous studies (H. Xiong et al., Bioorg Med Chem Lett, 23:6789–6793, 2013, http://dx.doi.org/10.1016/j.bmcl.2013.10.018; C. L. Tiong-Yip et al., Antimicrob Agents Chemother, 58:3867–3873, 2014, http://dx.doi.org/10.1128/AAC.02540-14) had identified a small-molecule inhibitor, AZ-27, that targets the L protein. In this study, we examined the effect of AZ-27 on different aspects of RSV polymerase activity. AZ-27 was found to inhibit equally both mRNA transcription and genome replication in cell-based minigenome assays, indicating that it inhibits a step common to both of these RNA synthesis processes. Analysis in an in vitro transcription run-on assay, containing RSV nucleocapsids, showed that AZ-27 inhibits synthesis of transcripts from the 3′ end of the genome to a greater extent than those from the 5′ end, indicating that it inhibits transcription initiation. Consistent with this finding, experiments that assayed polymerase activity on the promoter showed that AZ-27 inhibited transcription and replication initiation. The RSV polymerase also can utilize the promoter sequence to perform a back-priming reaction. Interestingly, addition of AZ-27 had no effect on the addition of up to three nucleotides by back-priming but inhibited further extension of the back-primed RNA. These data provide new information regarding the mechanism of inhibition by AZ-27. They also suggest that the RSV polymerase adopts different conformations to perform its different activities at the promoter. IMPORTANCE Currently, there are no effective antiviral drugs to treat RSV infection. The RSV polymerase is an

  1. 75 FR 38754 - Safety Zone; IJSBA World Finals; Lower Colorado River, Lake Havasu, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ... Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a public meeting. But you may... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; IJSBA World Finals; Lower Colorado River... River in support of the IJSBA World Finals. This temporary safety zone is necessary to provide for...

  2. 78 FR 34300 - Safety Zone; Bullhead City Regatta, Bullhead City, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-07

    ...The Coast Guard is proposing a temporary safety zone on the navigable waters of the Colorado River in Bullhead City, Arizona for the Bullhead City Regatta on August 10, 2013. This temporary safety zone is necessary to provide for the safety of the participants, crew, spectators, participating vessels, and other vessels and users of the waterway. Persons and vessels would be prohibited from......

  3. 76 FR 38568 - Safety Zone; Bullhead City Regatta, Bullhead City, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ...The Coast Guard is establishing a temporary safety zone on the navigable waters of the Colorado River in Bullhead City, Arizona for the Bullhead City Regatta on August 13, 2011. This temporary safety zone is necessary to provide for the safety of the participants, crew, spectators, participating vessels, and other vessels and users of the waterway. Persons and vessels would be prohibited from......

  4. 78 FR 17869 - Safety Zone; Desert Storm Shootout; Lake Havasu, Lake Havasu City, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ... FR Federal Register NPRM Notice of Proposed Rulemaking A. Regulatory History and Information The... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Desert Storm Shootout; Lake Havasu, Lake... establishing a temporary safety zone on the navigable waters of the Colorado River in Lake Havasu, Lake...

  5. Discovery and antiparasitic activity of AZ960 as a Trypanosoma brucei ERK8 inhibitor.

    PubMed

    Valenciano, Ana L; Ramsey, Aaron C; Santos, Webster L; Mackey, Zachary B

    2016-10-01

    Human African trypanosomiasis (HAT) is a lethal, vector-borne disease caused by the parasite Trypanosoma brucei. Therapeutic strategies for this neglected tropical disease suffer from disadvantages such as toxicity, high cost, and emerging resistance. Therefore, new drugs with novel modes of action are needed. We screened cultured T. brucei against a focused kinase inhibitor library to identify promising bioactive compounds. Among the ten hits identified from the phenotypic screen, AZ960 emerged as the most promising compound with potent antiparasitic activity (IC50=120nM) and was shown to be a selective inhibitor of an essential gene product, T. brucei extracellular signal-regulated kinase 8 (TbERK8). We report that AZ960 has a Ki of 1.25μM for TbERK8 and demonstrate its utility in establishing TbERK8 as a potentially druggable target in T. brucei. PMID:27519462

  6. 75 FR 26098 - Safety Zone; Under Water Clean Up of Copper Canyon, Lake Havasu, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-11

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Under Water Clean Up of Copper Canyon, Lake... Clean up, which will involve 40 divers cleaning the river bottom in Lake Havasu. The Coast Guard is... responsibilities between the Federal Government and Indian tribes. Energy Effects We have analyzed this rule...

  7. 75 FR 64708 - Reorganization of Foreign-Trade Zone 75 under Alternative Site Framework; Phoenix, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-20

    ... Board adopted the alternative site framework (ASF) in December 2008 (74 FR 1170, 01/12/09; correction 74 FR 3987, 01/22/09) as an option for the establishment or reorganization of general-purpose zones... as magnet sites; Whereas, notice inviting public comment was given in the Federal Register (75...

  8. 78 FR 44011 - Safety Zone; Bullhead City Regatta; Bullhead City, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    .... SUPPLEMENTARY INFORMATION: ] Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM... Proposed Rulemaking for this event on June 7, 2013 (78 FR 34300). We received no comments or requests for a..., and excessive trash resulting from the event. The safety zone is being established this year as...

  9. 78 FR 23135 - Safety Zone; Blue Water Resort & Casino West Coast Nationals; Parker, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-18

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Blue Water Resort & Casino West Coast... River in Parker, Arizona for the Blue Water Resort & Casino West Coast Nationals. This temporary...

  10. 75 FR 61619 - Safety Zone; IJSBA World Finals, Lower Colorado River, Lake Havasu, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ... the Federal Register (75 FR 38754). We received no comments on the proposed rule. No public meeting... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; IJSBA World Finals, Lower Colorado River... Arizona in support of the International Jet Sports Boating Association (IJSBA) World Finals....

  11. 76 FR 61261 - Safety Zone; IJSBA World Finals; Lower Colorado River, Lake Havasu, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-04

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; IJSBA World Finals; Lower Colorado River... support of the International Jet Sports Boating Association (IJSBA) World Finals. This temporary safety... The International Jet Sports Boating Association is sponsoring the IJSBA World Finals. The event...

  12. 77 FR 36439 - Safety Zone; Bullhead City Regatta; Bullhead City, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-19

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice... public dockets in the January 17, 2008, issue of the Federal Register (73 FR 3316). 4. Public Meeting We... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Bullhead City Regatta; Bullhead City,...

  13. Hair cells use active zones with different voltage dependence of Ca2+ influx to decompose sounds into complementary neural codes.

    PubMed

    Ohn, Tzu-Lun; Rutherford, Mark A; Jing, Zhizi; Jung, Sangyong; Duque-Afonso, Carlos J; Hoch, Gerhard; Picher, Maria Magdalena; Scharinger, Anja; Strenzke, Nicola; Moser, Tobias

    2016-08-01

    For sounds of a given frequency, spiral ganglion neurons (SGNs) with different thresholds and dynamic ranges collectively encode the wide range of audible sound pressures. Heterogeneity of synapses between inner hair cells (IHCs) and SGNs is an attractive candidate mechanism for generating complementary neural codes covering the entire dynamic range. Here, we quantified active zone (AZ) properties as a function of AZ position within mouse IHCs by combining patch clamp and imaging of presynaptic Ca(2+) influx and by immunohistochemistry. We report substantial AZ heterogeneity whereby the voltage of half-maximal activation of Ca(2+) influx ranged over ∼20 mV. Ca(2+) influx at AZs facing away from the ganglion activated at weaker depolarizations. Estimates of AZ size and Ca(2+) channel number were correlated and larger when AZs faced the ganglion. Disruption of the deafness gene GIPC3 in mice shifted the activation of presynaptic Ca(2+) influx to more hyperpolarized potentials and increased the spontaneous SGN discharge. Moreover, Gipc3 disruption enhanced Ca(2+) influx and exocytosis in IHCs, reversed the spatial gradient of maximal Ca(2+) influx in IHCs, and increased the maximal firing rate of SGNs at sound onset. We propose that IHCs diversify Ca(2+) channel properties among AZs and thereby contribute to decomposing auditory information into complementary representations in SGNs. PMID:27462107

  14. Microstructure formation in partially melted zone during gas tungsten arc welding of AZ91 Mg cast alloy

    SciTech Connect

    Zhu Tianping Chen, Zhan W.; Gao Wei

    2008-11-15

    During gas tungsten arc (GTA) welding of AZ91 Mg cast alloy, constitutional liquid forms locally in the original interdendritic regions in the partially melted zone (PMZ). The PMZ re-solidification behaviour has not been well understood. In this study, the gradual change of the re-solidification microstructure within PMZ from base metal side to weld metal side was characterised. High cooling rate experiments using Gleeble thermal simulator were also conducted to understand the morphological change of the {alpha}-Mg/{beta}-Mg{sub 17}Al{sub 12} phase interface formed during re-solidification after partial melting. It was found that the original partially divorced eutectic structure has become a more regular eutectic phase in most of the PMZ, although close to the fusion boundary the re-solidified eutectic is again a divorced one. Proceeding the eutectic re-solidification, if the degree of partial melting is sufficiently high, {alpha}-Mg re-solidified with a cellular growth, resulting in a serrated interface between {alpha}-Mg and {alpha}-Mg/{beta}-Mg{sub 17}Al{sub 12} in the weld sample and between {alpha}-Mg and {beta}-Mg{sub 17}Al{sub 12} (fully divorced eutectic) in Gleeble samples. The morphological changes affected by the peak temperature and cooling rate are also explained.

  15. Rab3-GEF Controls Active Zone Development at the Drosophila Neuromuscular Junction123

    PubMed Central

    Bae, Haneui; Chen, Shirui; Roche, John P.; Ai, Minrong; Wu, Chunlai

    2016-01-01

    Abstract Synaptic signaling involves the release of neurotransmitter from presynaptic active zones (AZs). Proteins that regulate vesicle exocytosis cluster at AZs, composing the cytomatrix at the active zone (CAZ). At the Drosophila neuromuscular junction (NMJ), the small GTPase Rab3 controls the distribution of CAZ proteins across release sites, thereby regulating the efficacy of individual AZs. Here we identify Rab3-GEF as a second protein that acts in conjunction with Rab3 to control AZ protein composition. At rab3-GEF mutant NMJs, Bruchpilot (Brp) and Ca2+ channels are enriched at a subset of AZs, leaving the remaining sites devoid of key CAZ components in a manner that is indistinguishable from rab3 mutant NMJs. As the Drosophila homologue of mammalian DENN/MADD and Caenorhabditis elegans AEX-3, Rab3-GEF is a guanine nucleotide exchange factor (GEF) for Rab3 that stimulates GDP to GTP exchange. Mechanistic studies reveal that although Rab3 and Rab3-GEF act within the same mechanism to control AZ development, Rab3-GEF is involved in multiple roles. We show that Rab3-GEF is required for transport of Rab3. However, the synaptic phenotype in the rab3-GEF mutant cannot be fully explained by defective transport and loss of GEF activity. A transgenically expressed GTP-locked variant of Rab3 accumulates at the NMJ at wild-type levels and fully rescues the rab3 mutant but is unable to rescue the rab3-GEF mutant. Our results suggest that although Rab3-GEF acts upstream of Rab3 to control Rab3 localization and likely GTP-binding, it also acts downstream to regulate CAZ development, potentially as a Rab3 effector at the synapse. PMID:27022630

  16. Bruchpilot and Synaptotagmin collaborate to drive rapid glutamate release and active zone differentiation.

    PubMed

    Paul, Mila M; Pauli, Martin; Ehmann, Nadine; Hallermann, Stefan; Sauer, Markus; Kittel, Robert J; Heckmann, Manfred

    2015-01-01

    The active zone (AZ) protein Bruchpilot (Brp) is essential for rapid glutamate release at Drosophila melanogaster neuromuscular junctions (NMJs). Quantal time course and measurements of action potential-waveform suggest that presynaptic fusion mechanisms are altered in brp null mutants (brp(69) ). This could account for their increased evoked excitatory postsynaptic current (EPSC) delay and rise time (by about 1 ms). To test the mechanism of release protraction at brp(69) AZs, we performed knock-down of Synaptotagmin-1 (Syt) via RNAi (syt(KD) ) in wildtype (wt), brp(69) and rab3 null mutants (rab3(rup) ), where Brp is concentrated at a small number of AZs. At wt and rab3(rup) synapses, syt(KD) lowered EPSC amplitude while increasing rise time and delay, consistent with the role of Syt as a release sensor. In contrast, syt(KD) did not alter EPSC amplitude at brp(69) synapses, but shortened delay and rise time. In fact, following syt(KD) , these kinetic properties were strikingly similar in wt and brp(69) , which supports the notion that Syt protracts release at brp(69) synapses. To gain insight into this surprising role of Syt at brp(69) AZs, we analyzed the structural and functional differentiation of synaptic boutons at the NMJ. At 'tonic' type Ib motor neurons, distal boutons contain more AZs, more Brp proteins per AZ and show elevated and accelerated glutamate release compared to proximal boutons. The functional differentiation between proximal and distal boutons is Brp-dependent and reduced after syt(KD) . Notably, syt(KD) boutons are smaller, contain fewer Brp positive AZs and these are of similar number in proximal and distal boutons. In addition, super-resolution imaging via dSTORM revealed that syt(KD) increases the number and alters the spatial distribution of Brp molecules at AZs, while the gradient of Brp proteins per AZ is diminished. In summary, these data demonstrate that normal structural and functional differentiation of Drosophila AZs requires

  17. 76 FR 62760 - Foreign-Trade Zone 277-Western Maricopa County, AZ; Application for Temporary/Interim...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    .../Interim Manufacturing Authority; Sub-Zero, Inc.; (Refrigerators); Goodyear, AZ An application has been... (Site 3). Under T/IM procedures, Sub-Zero has requested authority to produce refrigerators (HTSUS 8418... 45% of the value of the finished refrigerators) include: ABS resin (HTSUS 3903.30), fittings...

  18. SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.

    PubMed

    Mochida, Sumiko; Hida, Yamato; Tanifuji, Shota; Hagiwara, Akari; Hamada, Shun; Abe, Manabu; Ma, Huan; Yasumura, Misato; Kitajima, Isao; Sakimura, Kenji; Ohtsuka, Toshihisa

    2016-09-13

    Short-term synaptic depression (STD) is a common form of activity-dependent plasticity observed widely in the nervous system. Few molecular pathways that control STD have been described, but the active zone (AZ) release apparatus provides a possible link between neuronal activity and plasticity. Here, we show that an AZ cytomatrix protein CAST and an AZ-associated protein kinase SAD-B coordinately regulate STD by controlling reloading of the AZ with release-ready synaptic vesicles. SAD-B phosphorylates the N-terminal serine (S45) of CAST, and S45 phosphorylation increases with higher firing rate. A phosphomimetic CAST (S45D) mimics CAST deletion, which enhances STD by delaying reloading of the readily releasable pool (RRP), resulting in a pool size decrease. A phosphonegative CAST (S45A) inhibits STD and accelerates RRP reloading. Our results suggest that the CAST/SAD-B reaction serves as a brake on synaptic transmission by temporal calibration of activity and synaptic depression via RRP size regulation. PMID:27626661

  19. Molecular Machines Regulating the Release Probability of Synaptic Vesicles at the Active Zone

    PubMed Central

    Körber, Christoph; Kuner, Thomas

    2016-01-01

    The fusion of synaptic vesicles (SVs) with the plasma membrane of the active zone (AZ) upon arrival of an action potential (AP) at the presynaptic compartment is a tightly regulated probabilistic process crucial for information transfer. The probability of a SV to release its transmitter content in response to an AP, termed release probability (Pr), is highly diverse both at the level of entire synapses and individual SVs at a given synapse. Differences in Pr exist between different types of synapses, between synapses of the same type, synapses originating from the same axon and even between different SV subpopulations within the same presynaptic terminal. The Pr of SVs at the AZ is set by a complex interplay of different presynaptic properties including the availability of release-ready SVs, the location of the SVs relative to the voltage-gated calcium channels (VGCCs) at the AZ, the magnitude of calcium influx upon arrival of the AP, the buffering of calcium ions as well as the identity and sensitivity of the calcium sensor. These properties are not only interconnected, but can also be regulated dynamically to match the requirements of activity patterns mediated by the synapse. Here, we review recent advances in identifying molecules and molecular machines taking part in the determination of vesicular Pr at the AZ. PMID:26973506

  20. Molecular Machines Regulating the Release Probability of Synaptic Vesicles at the Active Zone.

    PubMed

    Körber, Christoph; Kuner, Thomas

    2016-01-01

    The fusion of synaptic vesicles (SVs) with the plasma membrane of the active zone (AZ) upon arrival of an action potential (AP) at the presynaptic compartment is a tightly regulated probabilistic process crucial for information transfer. The probability of a SV to release its transmitter content in response to an AP, termed release probability (Pr), is highly diverse both at the level of entire synapses and individual SVs at a given synapse. Differences in Pr exist between different types of synapses, between synapses of the same type, synapses originating from the same axon and even between different SV subpopulations within the same presynaptic terminal. The Pr of SVs at the AZ is set by a complex interplay of different presynaptic properties including the availability of release-ready SVs, the location of the SVs relative to the voltage-gated calcium channels (VGCCs) at the AZ, the magnitude of calcium influx upon arrival of the AP, the buffering of calcium ions as well as the identity and sensitivity of the calcium sensor. These properties are not only interconnected, but can also be regulated dynamically to match the requirements of activity patterns mediated by the synapse. Here, we review recent advances in identifying molecules and molecular machines taking part in the determination of vesicular Pr at the AZ. PMID:26973506

  1. Serpentine in active subduction zones

    NASA Astrophysics Data System (ADS)

    Reynard, Bruno

    2013-09-01

    Serpentinization is a key phenomenon for understanding the geodynamics of subduction zones in the 10-200 km depth range. Serpentines are a major water carrier, and their rheological properties have a strong influence on deformation partitioning and seismicity at depths. I review experimental investigations that have been conducted on serpentines, with emphasis on the large body of data acquired over the past decade. Determinations of physical properties at the pressure and temperature conditions of subductions allow interpreting geophysical data in active subduction in terms of mineralogy and petrology, and to link the presence of serpentinites with deformation and fluid circulation. The fluid budget can be partially constrained from geophysical data. Elasticity data provide a quantitative basis for mapping serpentinization in the mantle wedge and slab from seismic tomography. Anisotropy suggests the existence of thin serpentinite channels above the plate interface, that account for mechanical decoupling inferred from down-dip limit of the seismogenic zone and heat flow. Strain-rate dependent rheology of antigorite serpentine is consistent with stable deformation of this thin layer or channel over timescales ranging from those of the seismic cycle to those of thermal equilibration and exhumation of high-pressure rocks, and with the geological record of subduction-related deformation. Circulation of serpentinizing fluids depends on the permeability structure, and is imaged by electrical conductivity tomography. It could be controlled by fracturing in the undeformed cold nose of the mantle wedge, and by plastic deformation along the plate interface. Fluid migration mechanisms are similar to those inferred from petrological and geochemical data on exhumed serpentinites. Estimation of the fluid budget associated with serpentine formation will rely on numerical simulations for which coupling of kinetics of hydration and dehydration at scales ranging from grain size up

  2. Piccolo Directs Activity Dependent F-Actin Assembly from Presynaptic Active Zones via Daam1

    PubMed Central

    Wagh, Dhananjay; Terry-Lorenzo, Ryan; Waites, Clarissa L.; Leal-Ortiz, Sergio A.; Maas, Christoph; Reimer, Richard J.; Garner, Craig C.

    2015-01-01

    The dynamic assembly of filamentous (F) actin plays essential roles in the assembly of presynaptic boutons, the fusion, mobilization and recycling of synaptic vesicles (SVs), and presynaptic forms of plasticity. However, the molecular mechanisms that regulate the temporal and spatial assembly of presynaptic F-actin remain largely unknown. Similar to other F-actin rich membrane specializations, presynaptic boutons contain a set of molecules that respond to cellular cues and trans-synaptic signals to facilitate activity-dependent assembly of F-actin. The presynaptic active zone (AZ) protein Piccolo has recently been identified as a key regulator of neurotransmitter release during SV cycling. It does so by coordinating the activity-dependent assembly of F-Actin and the dynamics of key plasticity molecules including Synapsin1, Profilin and CaMKII. The multidomain structure of Piccolo, its exquisite association with the AZ, and its ability to interact with a number of actin-associated proteins suggest that Piccolo may function as a platform to coordinate the spatial assembly of F-actin. Here we have identified Daam1, a Formin that functions with Profilin to drive F-actin assembly, as a novel Piccolo binding partner. We also found that within cells Daam1 activation promotes Piccolo binding, an interaction that can spatially direct the polymerization of F-Actin. Moreover, similar to Piccolo and Profilin, Daam1 loss of function impairs presynaptic-F-actin assembly in neurons. These data suggest a model in which Piccolo directs the assembly of presynaptic F-Actin from the AZ by scaffolding key actin regulatory proteins including Daam1. PMID:25897839

  3. Pluripotency Activity of Nanog Requires Biochemical Stabilization by Variant Histone Protein H2A.Z.

    PubMed

    Wang, Jiaxu; Qiao, Mengran; He, Qianqian; Shi, Ronghua; Loh, Sharon Jia Hui; Stanton, Lawrence W; Wu, Mian

    2015-07-01

    The variant histone protein H2A.Z plays a critical role in early development. Likewise, Nanog, a master regulator of embryonic stem cells (ESCs), is essential for proper development in early embryogenesis. In this study, we establish that these two factors work together to maintain pluripotency. It is shown that H2A.Z influences the protein level of Nanog through the ubiquitin-proteasome pathway. Knockdown of H2A.Z causes differentiation of mouse ESCs and disrupts the reprogramming of somatic cells, which can be partially rescued by overexpression of Nanog. We conclude that the H2A.Z-Nanog partnership is involved in ESC pluripotency and reprogramming of somatic cells. Stem Cells 2015;33:2126-2134. PMID:25809870

  4. Interim Report for Crucible-Scale Active Vitrification Testing Envelope B (AZ-102)

    SciTech Connect

    Crawford, C.L.

    2002-08-23

    The purposes of this work were to demonstrate the evaporation of AZ-102 supernate, demonstrate the vitrification of the evaporated concentrate in a crucible melt, and to demonstrate acceptance of the resulting glass by analysis (chemical and radionuclides) and durability testing.

  5. 77 FR 75144 - Foreign-Trade Zone 277-Western Maricopa County, AZ; Application for Expansion; (New Magnet Site...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-19

    ... Board on December 22, 2010 (Board Order 1733, 76 FR 1134, 01/07/2011) and was reorganized under the ASF on December 16, 2011 (Board Order 1804, 76 FR 80886, 12/27/2011). The zone project currently has a...; (New Magnet Site) Under Alternative Site Framework An application has been submitted to the...

  6. 77 FR 52681 - Reorganization and Expansion of Foreign-Trade Zone 219 Under Alternative Site Framework Yuma, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-30

    ..., the Board adopted the alternative site framework (ASF) (74 FR 1170, 01/12/09; correction 74 FR 3987, 01/22/09; 75 FR 71069-71070, 11/22/10) as an option for the establishment or reorganization of zones...-driven site (Site 5); Whereas, notice inviting public comment was given in the Federal Register (77...

  7. A high affinity RIM-binding protein/Aplip1 interaction prevents the formation of ectopic axonal active zones

    PubMed Central

    Siebert, Matthias; Böhme, Mathias A; Driller, Jan H; Babikir, Husam; Mampell, Malou M; Rey, Ulises; Ramesh, Niraja; Matkovic, Tanja; Holton, Nicole; Reddy-Alla, Suneel; Göttfert, Fabian; Kamin, Dirk; Quentin, Christine; Klinedinst, Susan; Andlauer, Till FM; Hell, Stefan W; Collins, Catherine A; Wahl, Markus C; Loll, Bernhard; Sigrist, Stephan J

    2015-01-01

    Synaptic vesicles (SVs) fuse at active zones (AZs) covered by a protein scaffold, at Drosophila synapses comprised of ELKS family member Bruchpilot (BRP) and RIM-binding protein (RBP). We here demonstrate axonal co-transport of BRP and RBP using intravital live imaging, with both proteins co-accumulating in axonal aggregates of several transport mutants. RBP, via its C-terminal Src-homology 3 (SH3) domains, binds Aplip1/JIP1, a transport adaptor involved in kinesin-dependent SV transport. We show in atomic detail that RBP C-terminal SH3 domains bind a proline-rich (PxxP) motif of Aplip1/JIP1 with submicromolar affinity. Pointmutating this PxxP motif provoked formation of ectopic AZ-like structures at axonal membranes. Direct interactions between AZ proteins and transport adaptors seem to provide complex avidity and shield synaptic interaction surfaces of pre-assembled scaffold protein transport complexes, thus, favouring physiological synaptic AZ assembly over premature assembly at axonal membranes. DOI: http://dx.doi.org/10.7554/eLife.06935.001 PMID:26274777

  8. Star and Dust Formation Activities in AzTEC-3: A Starburst Galaxy at z=5.3

    NASA Technical Reports Server (NTRS)

    Dwek, Eliahu

    2011-01-01

    Analyses of of high-redshift ultraluminous infrared (IR) galaxies traditionally use the observed optical to submillimeter spectral energy distribution (SED) and estimates of the dynamical mass as observational constraints to derive the star formation rate (SFR), the stellar mass, and age of these objects. In this lecture we add this constraint to the analysis of AzTEC-3, a starburst galaxy at z=5.3. We construct different stellar and chemical evolutionary scenarios, constrained to produce the inferred dust mass and observed luminosity before the associated stellar mass exceeds the observational limit. A robust result of our models is that all scenarios require most of the radiating dust mass to have been accreted in molecular clouds. Our new procedure highlights the importance of a multiwavelength approach, and of the use of dust evolution models in constraining the age and the star formation activity and history in galaxies.

  9. Star and Dust Formation Activities in AzTEC-3: A Starburst Galaxy at z equals 5.3

    NASA Technical Reports Server (NTRS)

    Dwek, Eliahu

    2011-01-01

    Analyses of of high-redshift ultraluminous infrared (IR) galaxies traditionally use the observed optical to submillimeter spectral energy distribution (SED) and estimates of the dynamical mass as observational constraints to derive the star formation rate (SFR), the stellar mass, and age of these objects. In this lecture we add this constraint to the analysis of AzTEC-3, a starburst galaxy at z=5.3. We construct different stellar and chemical evolutionary scenarios, constrained to produce the inferred dust mass and observed luminosity before the associated stellar mass exceeds the observational limit. A robust result of our models is that all scenarios require most of the radiating dust mass to have been accreted in molecular clouds. Our new procedure highlights the importance of a multi wavelength approach, and of the use of dust evolution models in constraining the age and the star formation activity and history in galaxies.

  10. Star Dust Formation Activities in AzTEC-3: A Starburst Galaxy at z=5.3

    NASA Technical Reports Server (NTRS)

    Dwek, Eliahu

    2011-01-01

    Analyses of of high-redshift ultraluminous infrared OR) galaxies traditionally use the observed optical to submillimeter spectral energy distribution (SED) and estimates of the dynamical mass as observational constraints to derive the star formation rate (SFR), the stellar mass, and age of these objects. In this lecture we add this constraint to the analysis of AzTEC-3, a starburst galaxy at z=5.3. We construct different stellar and chemical evolutionary scenarios, constrained to produce the inferred dust mass and observed luminosity before the associated stellar mass exceeds the observational limit. A robust result of our models is that all scenarios require most of the radiating dust mass to have been accreted in molecular clouds. Our new procedure highlights the importance of a multi wavelength approach, and of the use of dust evolution models in constraining the age and the star formation activity and history in galaxies.

  11. STAR AND DUST FORMATION ACTIVITIES IN AzTEC-3, A STARBURST GALAXY AT z = 5.3

    SciTech Connect

    Dwek, Eli; Staguhn, Johannes G.; Arendt, Richard G.; Benford, Dominic J.; Fixsen, Dale; Maher, Stephen F.; Moseley, Samuel H.; Sharp, Elmer H.; Capak, Peter L.; Kovacs, Attila; Karim, Alexander; Schinnerer, Eva; Leclercq, Samuel

    2011-09-01

    Analyses of high-redshift ultraluminous infrared (IR) galaxies traditionally use the observed optical to submillimeter spectral energy distribution (SED) and estimates of the dynamical mass as observational constraints to derive the star formation rate (SFR), the stellar mass, and age of these objects. An important observational constraint neglected in the analysis is the mass of dust giving rise to the IR emission. In this paper we add this constraint to the analysis of AzTEC-3. Adopting an upper limit to the mass of stars and a bolometric luminosity for this object, we construct different stellar and chemical evolutionary scenarios, constrained to produce the inferred dust mass and observed luminosity before the associated stellar mass exceeds the observational limit. We use the PEGASE population synthesis code and a chemical evolution model to follow the evolution of the galaxy's SED and its stellar and dust masses as a function of galactic age for seven different stellar initial mass functions (IMFs). We find that the model with a Top Heavy IMF provided the most plausible scenario consistent with the observational constraints. In this scenario the dust formed over a period of {approx}200 Myr, with an SFR of {approx}500 M{sub sun} yr{sup -1}. These values for the age and SFR in AzTEC-3 are significantly higher and lower, respectively, from those derived without the dust mass constraint. However, this scenario is not unique, and others cannot be completely ruled out because of the prevailing uncertainties in the age of the galaxy, its bolometric luminosity, and its stellar and dust masses. A robust result of our models is that all scenarios require most of the radiating dust mass to have been accreted in molecular clouds. Our new procedure highlights the importance of a multiwavelength approach, and of the use of dust evolution models in constraining the age and the star formation activity and history in galaxies.

  12. Pump Jet Mixing and Pipeline Transfer Assessment for High-Activity Radioactive Wastes in Hanford Tank 241-AZ-102

    SciTech Connect

    Y Onishi; KP Recknagle; BE Wells

    2000-08-09

    The authors evaluated how well two 300-hp mixer pumps would mix solid and liquid radioactive wastes stored in Hanford double-shell Tank 241-AZ-102 (AZ-102) and confirmed the adequacy of a three-inch (7.6-cm) pipeline system to transfer the resulting mixed waste slurry to the AP Tank Farm and a planned waste treatment (vitrification) plant on the Hanford Site. Tank AZ-102 contains 854,000 gallons (3,230 m{sup 3}) of supernatant liquid and 95,000 gallons (360 m{sup 3}) of sludge made up of aging waste (or neutralized current acid waste). The study comprises three assessments: waste chemistry, pump jet mixing, and pipeline transfer. The waste chemical modeling assessment indicates that the sludge, consisting of the solids and interstitial solution, and the supernatant liquid are basically in an equilibrium condition. Thus, pump jet mixing would not cause much solids precipitation and dissolution, only 1.5% or less of the total AZ-102 sludge. The pump jet mixing modeling indicates that two 300-hp mixer pumps would mobilize up to about 23 ft (7.0 m) of the sludge nearest the pump but would not erode the waste within seven inches (0.18 m) of the tank bottom. This results in about half of the sludge being uniformly mixed in the tank and the other half being unmixed (not eroded) at the tank bottom.

  13. Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone

    PubMed Central

    Gundelfinger, Eckart D.; Reissner, Carsten; Garner, Craig C.

    2016-01-01

    Bassoon and Piccolo are two very large scaffolding proteins of the cytomatrix assembled at the active zone (CAZ) where neurotransmitter is released. They share regions of high sequence similarity distributed along their entire length and seem to share both overlapping and distinct functions in organizing the CAZ. Here, we survey our present knowledge on protein-protein interactions and recent progress in understanding of molecular functions of these two giant proteins. These include roles in the assembly of active zones (AZ), the localization of voltage-gated Ca2+ channels (VGCCs) in the vicinity of release sites, synaptic vesicle (SV) priming and in the case of Piccolo, a role in the dynamic assembly of the actin cytoskeleton. Piccolo and Bassoon are also important for the maintenance of presynaptic structure and function, as well as for the assembly of CAZ specializations such as synaptic ribbons. Recent findings suggest that they are also involved in the regulation activity-dependent communication between presynaptic boutons and the neuronal nucleus. Together these observations suggest that Bassoon and Piccolo use their modular structure to organize super-molecular complexes essential for various aspects of presynaptic function. PMID:26793095

  14. Cellular and Pectin Dynamics during Abscission Zone Development and Ripe Fruit Abscission of the Monocot Oil Palm.

    PubMed

    Roongsattham, Peerapat; Morcillo, Fabienne; Fooyontphanich, Kim; Jantasuriyarat, Chatchawan; Tragoonrung, Somvong; Amblard, Philippe; Collin, Myriam; Mouille, Gregory; Verdeil, Jean-Luc; Tranbarger, Timothy J

    2016-01-01

    The oil palm (Elaeis guineensis Jacq.) fruit primary abscission zone (AZ) is a multi-cell layered boundary region between the pedicel (P) and mesocarp (M) tissues. To examine the cellular processes that occur during the development and function of the AZ cell layers, we employed multiple histological and immunohistochemical methods combined with confocal, electron and Fourier-transform infrared (FT-IR) microspectroscopy approaches. During early fruit development and differentiation of the AZ, the orientation of cell divisions in the AZ was periclinal compared with anticlinal divisions in the P and M. AZ cell wall width increased earlier during development suggesting cell wall assembly occurred more rapidly in the AZ than the adjacent P and M tissues. The developing fruit AZ contain numerous intra-AZ cell layer plasmodesmata (PD), but very few inter-AZ cell layer PD. In the AZ of ripening fruit, PD were less frequent, wider, and mainly intra-AZ cell layer localized. Furthermore, DAPI staining revealed nuclei are located adjacent to PD and are remarkably aligned within AZ layer cells, and remain aligned and intact after cell separation. The polarized accumulation of ribosomes, rough endoplasmic reticulum, mitochondria, and vesicles suggested active secretion at the tip of AZ cells occurred during development which may contribute to the striated cell wall patterns in the AZ cell layers. AZ cells accumulated intracellular pectin during development, which appear to be released and/or degraded during cell separation. The signal for the JIM5 epitope, that recognizes low methylesterified and un-methylesterified homogalacturonan (HG), increased in the AZ layer cell walls prior to separation and dramatically increased on the separated AZ cell surfaces. Finally, FT-IR microspectroscopy analysis indicated a decrease in methylesterified HG occurred in AZ cell walls during separation, which may partially explain an increase in the JIM5 epitope signal. The results obtained

  15. Cellular and Pectin Dynamics during Abscission Zone Development and Ripe Fruit Abscission of the Monocot Oil Palm

    PubMed Central

    Roongsattham, Peerapat; Morcillo, Fabienne; Fooyontphanich, Kim; Jantasuriyarat, Chatchawan; Tragoonrung, Somvong; Amblard, Philippe; Collin, Myriam; Mouille, Gregory; Verdeil, Jean-Luc; Tranbarger, Timothy J.

    2016-01-01

    The oil palm (Elaeis guineensis Jacq.) fruit primary abscission zone (AZ) is a multi-cell layered boundary region between the pedicel (P) and mesocarp (M) tissues. To examine the cellular processes that occur during the development and function of the AZ cell layers, we employed multiple histological and immunohistochemical methods combined with confocal, electron and Fourier-transform infrared (FT-IR) microspectroscopy approaches. During early fruit development and differentiation of the AZ, the orientation of cell divisions in the AZ was periclinal compared with anticlinal divisions in the P and M. AZ cell wall width increased earlier during development suggesting cell wall assembly occurred more rapidly in the AZ than the adjacent P and M tissues. The developing fruit AZ contain numerous intra-AZ cell layer plasmodesmata (PD), but very few inter-AZ cell layer PD. In the AZ of ripening fruit, PD were less frequent, wider, and mainly intra-AZ cell layer localized. Furthermore, DAPI staining revealed nuclei are located adjacent to PD and are remarkably aligned within AZ layer cells, and remain aligned and intact after cell separation. The polarized accumulation of ribosomes, rough endoplasmic reticulum, mitochondria, and vesicles suggested active secretion at the tip of AZ cells occurred during development which may contribute to the striated cell wall patterns in the AZ cell layers. AZ cells accumulated intracellular pectin during development, which appear to be released and/or degraded during cell separation. The signal for the JIM5 epitope, that recognizes low methylesterified and un-methylesterified homogalacturonan (HG), increased in the AZ layer cell walls prior to separation and dramatically increased on the separated AZ cell surfaces. Finally, FT-IR microspectroscopy analysis indicated a decrease in methylesterified HG occurred in AZ cell walls during separation, which may partially explain an increase in the JIM5 epitope signal. The results obtained

  16. The Active and Periactive Zone Organization and the Functional Properties of Small and Large Synapses

    PubMed Central

    Cano, Raquel; Tabares, Lucia

    2016-01-01

    The arrival of an action potential (AP) at a synaptic terminal elicits highly synchronized quanta release. Repetitive APs produce successive synaptic vesicle (SV) fusions that require management of spent SV components in the presynaptic membrane with minimum disturbance of the secretory apparatus. To this end, the synaptic machinery is structured accordingly to the strength and the range of frequencies at which each particular synapse operates. This results in variations in the number and dimension of Active Zones (AZs), amount and distribution of SVs, and probably, in the primary endocytic mechanisms they use. Understanding better how these structural differences determine the functional response in each case has been a matter of long-term interest. Here we review the structural and functional properties of three distinct types of synapses: the neuromuscular junction (NMJ; a giant, highly reliable synapse that must exocytose a large number of quanta with each stimulus to guarantee excitation of the postsynaptic cell), the hippocampal excitatory small synapse (which most often has a single release site and a relatively small pool of vesicles), and the cerebellar mossy fiber-granule cell synapse (which possesses hundreds of release sites and is able to translocate, dock and prime vesicles at high speed). We will focus on how the release apparatus is organized in each case, the relative amount of vesicular membrane that needs to be accommodated within the periAZ upon stimulation, the different mechanisms for retrieving the excess of membrane and finally, how these factors may influence the functioning of the release sites. PMID:27252645

  17. The Active and Periactive Zone Organization and the Functional Properties of Small and Large Synapses.

    PubMed

    Cano, Raquel; Tabares, Lucia

    2016-01-01

    The arrival of an action potential (AP) at a synaptic terminal elicits highly synchronized quanta release. Repetitive APs produce successive synaptic vesicle (SV) fusions that require management of spent SV components in the presynaptic membrane with minimum disturbance of the secretory apparatus. To this end, the synaptic machinery is structured accordingly to the strength and the range of frequencies at which each particular synapse operates. This results in variations in the number and dimension of Active Zones (AZs), amount and distribution of SVs, and probably, in the primary endocytic mechanisms they use. Understanding better how these structural differences determine the functional response in each case has been a matter of long-term interest. Here we review the structural and functional properties of three distinct types of synapses: the neuromuscular junction (NMJ; a giant, highly reliable synapse that must exocytose a large number of quanta with each stimulus to guarantee excitation of the postsynaptic cell), the hippocampal excitatory small synapse (which most often has a single release site and a relatively small pool of vesicles), and the cerebellar mossy fiber-granule cell synapse (which possesses hundreds of release sites and is able to translocate, dock and prime vesicles at high speed). We will focus on how the release apparatus is organized in each case, the relative amount of vesicular membrane that needs to be accommodated within the periAZ upon stimulation, the different mechanisms for retrieving the excess of membrane and finally, how these factors may influence the functioning of the release sites. PMID:27252645

  18. Molecular Mechanism of Active Zone Organization at Vertebrate Neuromuscular Junctions

    PubMed Central

    Nishimune, Hiroshi

    2013-01-01

    Organization of presynaptic active zones is essential for development, plasticity, and pathology of the nervous system. Recent studies indicate a trans-synaptic molecular mechanism that organizes the active zones by connecting the pre- and the postsynaptic specialization. The presynaptic component of this trans-synaptic mechanism is comprised of cytosolic active zone proteins bound to the cytosolic domains of voltage-dependent calcium channels (P/Q-, N-, and L-type) on the presynaptic membrane. The postsynaptic component of this mechanism is the synapse organizer (laminin β2) that is expressed by the postsynaptic cell and accumulates specifically on top of the postsynaptic specialization. The pre- and the postsynaptic components interact directly between the extracellular domains of calcium channels and laminin β2 to anchor the presynaptic protein complex in front of the postsynaptic specialization. Hence, the presynaptic calcium channel functions as a scaffolding protein for active zone organization and as an ion-conducting channel for synaptic transmission. In contrast to the requirement of calcium influx for synaptic transmission, the formation of the active zone does not require the calcium influx through the calcium channels. Importantly, the active zones of adult synapses are not stable structures and require maintenance for their integrity. Furthermore, aging or diseases of the central and peripheral nervous system impair the active zones. This review will focus on the molecular mechanisms that organize the presynaptic active zones and summarize recent findings at the neuromuscular junctions and other synapses. PMID:22135013

  19. The presynaptic dense projection of the Caenorhabditis elegans cholinergic neuromuscular junction localizes synaptic vesicles at the active zone through SYD-2/liprin and UNC-10/RIM-dependent interactions.

    PubMed

    Stigloher, Christian; Zhan, Hong; Zhen, Mei; Richmond, Janet; Bessereau, Jean-Louis

    2011-03-23

    The active zone (AZ) of chemical synapses is a specialized area of the presynaptic bouton in which vesicles fuse with the plasma membrane and release neurotransmitters. Efficient signaling requires synaptic vesicles (SVs) to be recruited, primed, and retained at the AZ, in close proximity to voltage-dependent calcium channels that are activated during presynaptic depolarization. The electron-dense specializations at the AZ might provide a molecular platform for the spatial coordination of these different processes. To investigate this hypothesis, we examined high-resolution three-dimensional models of Caenorhabditis elegans cholinergic neuromuscular junctions generated by electron tomography. First, we found that SVs are interconnected within the bouton by filaments similar to those described in vertebrates. Second, we resolved the three-dimensional structure of the dense projection centered in the AZ. The dense projection is a more complex structure than previously anticipated, with filaments radiating from a core structure that directly contact SVs in the interior of the bouton as well as SVs docked at the plasma membrane. Third, we investigated the functional correlate of these contacts by analyzing mutants disrupting two key AZ proteins: UNC-10/RIM and SYD-2/liprin. In both mutants, the number of contacts between SVs and the dense projection was significantly reduced. Similar to unc-10 mutants, the dependence of SV fusion on extracellular calcium concentration was exacerbated in syd-2 mutants when compared with the wild type. Hence, we propose that the dense projection ensures proper coupling of primed vesicles with calcium signaling by retaining them at the AZ via UNC-10/RIM and SYD-2/liprin-dependent mechanisms. PMID:21430140

  20. Active zones of mammalian neuromuscular junctions: formation, density, and aging

    PubMed Central

    Nishimune, Hiroshi

    2012-01-01

    Presynaptic active zones are synaptic vesicle release sites that playessential roles in the function and pathology of mammalian neuromuscular junctions (NMJs). The molecular mechanisms of active zone organization utilize presynaptic voltage-dependent calcium channels (VDCCs) in NMJs as scaffolding proteins. VDCCs interact extracellularly with the muscle-derived synapse organizer, laminin β2, and interact intracellularly with active zone-specific proteins, such as Bassoon, CAST/Erc2/ELKS2alpha, ELKS, Piccolo, and RIMs. These molecular mechanisms are supported by studies in P/Q- and N-type VDCCs double-knockout mice, and they are consistent with the pathological conditions of Lambert-Eaton myasthenic syndrome and Pierson syndrome, which are caused by autoantibodies against VDCCs or by a laminin β2 mutation. During normal postnatal maturation, NMJs maintain the density of active zones, while NMJs triple their size. However, active zones become impaired during aging. Propitiously, muscle exercise ameliorates the active zone impairment in aged NMJs, which suggests the potential for therapeutic strategies. PMID:23252894

  1. Synaptophysin 1 Clears Synaptobrevin 2 from the Presynaptic Active Zone to Prevent Short-Term Depression.

    PubMed

    Rajappa, Rajit; Gauthier-Kemper, Anne; Böning, Daniel; Hüve, Jana; Klingauf, Jürgen

    2016-02-16

    Release site clearance is an important process during synaptic vesicle (SV) recycling. However, little is known about its molecular mechanism. Here we identify self-assembly of exocytosed Synaptobrevin 2 (Syb2) and Synaptophysin 1 (Syp1) by homo- and hetero-oligomerization into clusters as key mechanisms mediating release site clearance for preventing cis-SNARE complex formation at the active zone (AZ). In hippocampal neurons from Syp1 knockout mice, neurons expressing a monomeric Syb2 mutant, or after acute block of the ATPase N-ethylmaleimide-sensitive factor (NSF), responsible for cis-SNARE complex disassembly, we found strong frequency-dependent short-term depression (STD), whereas retrieval of Syb2 by compensatory endocytosis was only affected weakly. Defects in Syb2 endocytosis were stimulus- and frequency-dependent, indicating that Syp1 is not essential for Syb2 retrieval, but for its efficient clearance upstream of endocytosis. Our findings identify an SV protein as a release site clearance factor. PMID:26854222

  2. Is There any Relationship Between Active Tabriz Fault Zone and Bozkush Fault Zones, NW Iran?

    NASA Astrophysics Data System (ADS)

    ISIK, V.; Saber, R.; Caglayan, A.

    2012-12-01

    Tectonic plate motions and consequent earthquakes can be actively observed along the northwestern Iran. The Tabriz fault zone (TFZ), also called the North Tabriz fault, active right-lateral strike-slip fault zone with slip rates estimated as ~8 mm/yr, has been vigorously deforming much of northwestern Iran for over the past several million years. Historical earthquakes on the TFZ consist of large magnitude, complimentary rupture length and changed the landscape of regions surrounding the fault zone. The TFZ in the city of Bostanabad is more segmented with several strands and joined by a series of WNW-ESE trending faults, called the Bozkush fault zones. The Bozkush fault zones (BFZ's) (south and north), bounding arch-shaped Bozkush mountains, generates not only hundreds of small earthquakes each year but also has provided significant earthquakes that have been historically documented. The rock units deformed within the BFZ's include Eocene-Oligocene volcanic rocks with intercalation limestone, Oligo-Miocene clastic rocks with intercalation gypsiferous marl and Plio-Quaternary volcano-sedimentary rocks, travertine and alluvium. The North and South Bozkush fault zones are characterized by development of structures typically associated with transpression. These include right-lateral strike-slip faults, thrust faults and foldings. Our field studies indicate that these zones include step to sub-vertical fault surfaces trending NW and NE with slickenlines. Slickensides preserve brittle kinematic indicators (e.g., Riedel shear patterns, slickenside marks) suggesting both dextral displacements and top-to-the-NE/NW and-SE/SW sense of shearing. Besides, mesoscopic and microscopic ductile kinematic indicators (e.g., asymmetric porphyroclasts, C/S fabrics) within Miocene gypsum marl show dextral displacements. Fault rocks along most of these faults consist of incohesive fault breccia and gauge. Adjacent to the fault contact evidence of bedding in Oligo-Miocene and Plio

  3. Triggered tremors beneath the seismogenic zone of an active fault zone, Kyushu, Japan

    NASA Astrophysics Data System (ADS)

    Miyazaki, Masahiro; Matsumoto, Satoshi; Shimizu, Hiroshi

    2015-11-01

    Non-volcanic tremors were induced by the surface waves of the 2012 Sumatra earthquake around the Hinagu fault zone in Kyushu, Japan. We inferred from dense seismic observation data that the hypocenters of these tremors were located beneath the seismogenic zone of the Hinagu fault. Focal mechanisms of the tremors were estimated using S-wave polarization angles. The estimated focal mechanisms show similarities to those of shallow earthquakes in this region. In addition, one of the nodal planes of the focal mechanisms is almost parallel to the strike direction of the Hinagu fault. These observations suggest that the tremors were triggered at the deeper extension of the active fault zone under stress conditions similar to those in the shallower seismogenic region. A low-velocity anomaly beneath the hypocentral area of the tremors might be related to the tremor activity.

  4. Tank 241-AZ-101 and tank 241-AZ-102, airlift circulator operation vapor sampling and analysis plan

    SciTech Connect

    TEMPLETON, A.M.

    1999-06-02

    This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for vapor samples obtained during the operation of the tank 241-AZ-101 and 241-AZ-102 airlift circulators (ALCs). The purpose of the ALC operation is to support portions of the operational test procedure (OTP) for Project W-030 (OTP-W030-001) and to perform functional test in support of Project W-151. Project W-030 is the 241-A-702 ventilation upgrade project (241-AZ-702) and Project W-151 is the 241-AZ-101 Mixer Pump Test. The functional tests will check the operability of the tank 241-AZ-101 ALCs. Process Memo's No.2E98-082 and No.2E99-001 (LMHC 1999a, LMHC 1999b) direct the operation of the ALCs and the Industrial Hygiene monitoring respectively. A series of tests will be conducted in which the ALCs in tanks 241-AZ-101 and 241-AZ-102 will be operated at different air flow rates. Vapor samples will be obtained to determine constituents that may be present in the tank headspace during ALC operation at tanks 241-AZ-101 and 241-AZ-102 as the waste is disturbed. During the testing, vapor samples will be obtained from the headspace of tanks 241-AZ-101 and 241-AZ-102 via the unused port on the standard hydrogen monitoring system (SHMS). Results will be used to provide the waste feed delivery program with environmental air permitting data for tank waste disturbing activities. Because of radiological concerns, the samples will be filtered for particulates. It is recognized that this may remove some organic compounds.

  5. Structural Analysis of Active North Bozgush Fault Zone (NW Iran)

    NASA Astrophysics Data System (ADS)

    Saber, R.; Isik, V.; Caglayan, A.

    2013-12-01

    NW Iran is one of the seismically active regions between Zagros Thrust Belt at the south and Caucasus at the north. Not only large magnitude historical earthquakes (Ms>7), but also 1987 Bozgush, 1997 Ardebil (Mw 6.1) and 2012 Ahar-Varzagan (Mw 6.4) earthquakes reveal that the region is seismically active. The North Bozgush Fault Zone (NBFZ) in this region has tens of kilometers in length and hundreds of meters in width. The zone has produced some large and destructive earthquakes (1593 M:6.1 and 1883 M:6.2). The NBFZ affects the Cenozoic units and along this zone Eocene units thrusted over Miocene and/or Plio-Quaternary sedimentary units. Together with morphologic features (stream offsets and alluvial fan movements) affecting the young unites reveal that the zone is active. The zone is mainly characterized by strike-slip faults with reverse component and reverse faults. Reverse faults striking N55°-85°E and dip of 40°-50° to the SW while strike-slip faults show right lateral slip with N60°-85°W and N60°-80°E directions. Our structural data analysis in NBFZ indicates that the axis direction of σ2 principal stress is vertical and the stress ratio (R) is 0.12. These results suggest that the tectonic regime along the North Bozgush Fault Zone is transpressive. Obtained other principal stresses (σ1, σ3) results are compatible with stress directions and GPS velocity suggested for NW Iran.

  6. Rab3-interacting molecules 2α and 2β promote the abundance of voltage-gated CaV1.3 Ca2+ channels at hair cell active zones

    PubMed Central

    Jung, Sangyong; Oshima-Takago, Tomoko; Chakrabarti, Rituparna; Wong, Aaron B.; Jing, Zhizi; Yamanbaeva, Gulnara; Picher, Maria Magdalena; Wojcik, Sonja M.; Göttfert, Fabian; Predoehl, Friederike; Michel, Katrin; Hell, Stefan W.; Schoch, Susanne; Strenzke, Nicola; Wichmann, Carolin; Moser, Tobias

    2015-01-01

    Ca2+ influx triggers the fusion of synaptic vesicles at the presynaptic active zone (AZ). Here we demonstrate a role of Ras-related in brain 3 (Rab3)–interacting molecules 2α and β (RIM2α and RIM2β) in clustering voltage-gated CaV1.3 Ca2+ channels at the AZs of sensory inner hair cells (IHCs). We show that IHCs of hearing mice express mainly RIM2α, but also RIM2β and RIM3γ, which all localize to the AZs, as shown by immunofluorescence microscopy. Immunohistochemistry, patch-clamp, fluctuation analysis, and confocal Ca2+ imaging demonstrate that AZs of RIM2α-deficient IHCs cluster fewer synaptic CaV1.3 Ca2+ channels, resulting in reduced synaptic Ca2+ influx. Using superresolution microscopy, we found that Ca2+ channels remained clustered in stripes underneath anchored ribbons. Electron tomography of high-pressure frozen synapses revealed a reduced fraction of membrane-tethered vesicles, whereas the total number of membrane-proximal vesicles was unaltered. Membrane capacitance measurements revealed a reduction of exocytosis largely in proportion with the Ca2+ current, whereas the apparent Ca2+ dependence of exocytosis was unchanged. Hair cell-specific deletion of all RIM2 isoforms caused a stronger reduction of Ca2+ influx and exocytosis and significantly impaired the encoding of sound onset in the postsynaptic spiral ganglion neurons. Auditory brainstem responses indicated a mild hearing impairment on hair cell-specific deletion of all RIM2 isoforms or global inactivation of RIM2α. We conclude that RIM2α and RIM2β promote a large complement of synaptic Ca2+ channels at IHC AZs and are required for normal hearing. PMID:26034270

  7. 78 FR 4155 - Agency Information Collection Activities: Application for Foreign Trade Zone and/or Status...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-18

    ... Foreign Trade Zone and/or Status Designation, and Application for Foreign Trade Zone Activity Permit... Application for Foreign Trade Zone Admission and/or Status Designation, and Application for Foreign Trade Zone... Foreign Trade Zone Admission and/or Status Designation, and Application for Foreign Trade......

  8. Neurotransmitters couple brain activity to subventricular zone neurogenesis

    PubMed Central

    Young, Stephanie Z.; Taylor, M. Morgan; Bordey, Angélique

    2011-01-01

    Adult neurogenesis occurs in two privileged microenvironments, the hippocampal subgranular zone of the dentate gyrus and the subventricular zone (SVZ) along the lateral ventricle. This review focuses on accumulating evidence suggesting that the activity of specific brain regions or bodily states influences SVZ cell proliferation and neurogenesis. Neuromodulators such as dopamine and serotonin have been shown to have long-range effects through neuronal projections into the SVZ. Local GABA and glutamate signaling have demonstrated effects on SVZ proliferation and neurogenesis, but an extra-niche source of these neurotransmitters remains to be explored and options will be discussed. There is also accumulating evidence that diseases and bodily states such as Alzheimer's disease, seizures, sleep, and pregnancy influence SVZ cell proliferation. With such complex behavior and environmentally-driven factors that control subregion-specific activity, it will become necessary to account for overlapping roles of multiple neurotransmitter systems on neurogenesis when developing cell therapies or drug treatments. PMID:21395856

  9. Effects of the types of overlap on the mechanical properties of FSSW welded AZ series magnesium alloy joints

    NASA Astrophysics Data System (ADS)

    Wang, Dan; Shen, Jun; Wang, Lin-Zhi

    2012-03-01

    The effects of the types of overlap on the mechanical properties of the friction stir spot welding (FSSW) welded AZ series magnesium alloy joints were investigated by microstructural observations, microhardness tests, and tensile tests. The results show that the microstructure of the stir zone adjacent to the periphery of the rotating pin is mainly composed of the upper sheet. The average distance D between the longitudinal segment of the curved interface and the keyhole periphery, the tensile shear force, and the microhardness of the stir zone of the FSSW welded AZ61 alloy joint are the highest in all samples. During FSSW of AZ31 and AZ61 dissimilar magnesium alloys, the irregular deformation of the longitudinal segment of the curved interface appears, while the microhardness of the stir zone is higher when AZ61 alloy is the upper sheet. Moreover, the microhardness of the stir zone increases initially and then decreases sharply in the longitudinal test position.

  10. The Interfacial Transition Zone in Alkali-Activated Slag Mortars

    NASA Astrophysics Data System (ADS)

    San Nicolas, Rackel; Provis, John

    2015-12-01

    The interfacial transition zone (ITZ) is known to strongly influence the mechanical and transport properties of mortars and concretes. This paper studies the ITZ between siliceous (quartz) aggregates and alkali activated slag binders in the context of mortar specimens. Backscattered electron images (BSE) generated in an environmental scanning electron microscope (ESEM) are used to identify unreacted binder components, reaction products and porosity in the zone surrounding aggregate particles, by composition and density contrast. X-ray mapping is used to exclude the regions corresponding to the aggregates from the BSE image of the ITZ, thus enabling analysis of only the binder phases, which are segmented into binary images by grey level discrimination. A distinct yet dense ITZ region is present in the alkali-activated slag mortars, containing a reduced content of unreacted slag particles compared to the bulk binder. The elemental analysis of this region shows that it contains a (C,N)-A-S-H gel which seems to have a higher content of Na (potentially deposited through desiccation of the pore solution) and a lower content of Ca than the bulk inner and outer products forming in the main binding region. These differences are potentially important in terms of long-term concrete performance, as the absence of a highly porous interfacial transition zone region is expected to provide a positive influence on the mechanical and transport properties of alkali-activated slag concretes.

  11. 78 FR 16701 - Agency Information Collection Activities: Application for Foreign Trade Zone and/or Status...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-18

    ... Foreign Trade Zone and/or Status Designation, and Application for Foreign Trade Zone Activity Permit... approval in accordance with the Paperwork Reduction Act: Application for Foreign Trade Zone Admission and/or Status Designation, and Application for Foreign Trade Zone Activity Permit (CBP Forms 214,...

  12. Semi empirical hardness predictive model for AZ91 nanocomposite

    NASA Astrophysics Data System (ADS)

    Zaidi, N. H. A.; Jamaludin, S. B.; Zaidi, A. M. A.; Ahmad, K. R.

    2016-07-01

    AZ91 nanocomposite was exposed to several heat treatment processes and the effect of precipitation hardening on hardness was studied as a function of time and temperature. The investigation shows the significant of time and temperature are the main role in the precipitation hardening process of the nanocomposite. Kinetics study show a deceptive activation energy of 21 kJ/mol of the AZ91 nanocomposite. A relationship was derived to predict the maximum hardness at given time and temperature.

  13. Linking Plagioclase Zoning Patterns to Active Magma Processes

    NASA Astrophysics Data System (ADS)

    Izbekov, P. E.; Nicolaysen, K. P.; Neill, O. K.; Shcherbakov, V.; Plechov, P.; Eichelberger, J. C.

    2015-12-01

    Plagioclase, one of the most common and abundant mineral phases in volcanic products, will vary in composition in response to changes in temperature, pressure, composition of the ambient silicate melt, and melt H2O concentration. Changes in these parameters may cause dissolution or growth of plagioclase crystals, forming characteristic textural and compositional variations (zoning patterns), the complete core-to-rim sequence of which describes events experienced by an individual crystal from its nucleation to the last moments of its growth. Plagioclase crystals in a typical volcanic rock may look drastically dissimilar despite their spatial proximity and the fact that they have erupted together. Although they shared last moments of their growth during magma ascent and eruption, their prior experiences could be very different, as plagioclase crystals often come from different domains of the same magma system. Distinguishing similar zoning patterns, correlating them across the entire population of plagioclase crystals, and linking these patterns to specific perturbations in the magmatic system may provide additional perspective on the variety, extent, and timing of magma processes at active volcanic systems. Examples of magma processes, which may be distinguished based on plagioclase zoning patterns, include (1) cooling due to heat loss, (2) heating and/or pressure build up due to an input of new magmatic material, (3) pressure drop in response to magma system depressurization, and (4) crystal transfer between different magma domains/bodies. This review will include contrasting examples of zoning patters from recent eruptions of Karymsky, Bezymianny, and Tolbachik Volcanoes in Kamchatka, Augustine and Cleveland Volcanoes in Alaska, as well as from the drilling into an active magma body at Krafla, Iceland.

  14. Tank 241-AZ-101 and Tank 241-AZ-102 Airlift Circulator Operation Vapor Sampling and Analysis Plan

    SciTech Connect

    TEMPLETON, A.M.

    1999-12-07

    This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for vapor samples obtained during the operation of the tank 241-AZ-101 and 241-AZ-102 airlift circulators (ALCs) and during the initial operation (''bump'') of the tank 241-AZ-101 mixer pumps. The purpose of the ALC operation is to support portions of the operational test procedure (OTP) for Project W-030 (OTP-W030-001) and to perform functional test in support of Project W-151. Project W-030 is the 241-A-702 ventilation upgrade project (241-142-702) and Project W-151 is the 241-AZ-101 Mixer Pump Test. The functional tests will check the operability of the tank 241-AZ-101 ALCs. Process Memo's No. 2E98-082 and No. 2E99-001 (LMHC 1999a, LMHC 1999b) direct the operation of the ALCs and the Industrial Hygiene monitoring respectively. A series of tests will be conducted in which the ALCs in tanks 241-AZ-101 and 241-AZ-102 will be operated at different air flow rates. Vapor samples will be obtained to determine constituents that may be present in the tank headspace during ALC operation at tanks 241-AZ-101 and 241-AZ-102 as the waste is disturbed. During the testing, vapor samples will be obtained from the headspace of tanks 241-AZ-101 and 241-AZ-102 via the unused port on the standard hydrogen monitoring system (SHMS). In addition the last two vapor samples will be collected from the headspace of tank 241-AZ-101 during the operation of the mixer pumps. Each mixer pump will be operated for approximately 5 minutes. Results will be used to provide the waste feed delivery program with environmental air permitting data for tank waste disturbing activities. Because of radiological concerns, the samples will be filtered for particulates. It is recognized that this may remove some organic compounds. The following sections provide the general methodology and procedures to be used in the preparation, retrieval

  15. Constitutive turnover of histone H2A.Z at yeast promoters requires the preinitiation complex

    PubMed Central

    Tramantano, Michael; Sun, Lu; Au, Christy; Labuz, Daniel; Liu, Zhimin; Chou, Mindy; Shen, Chen; Luk, Ed

    2016-01-01

    The assembly of the preinitiation complex (PIC) occurs upstream of the +1 nucleosome which, in yeast, obstructs the transcription start site and is frequently assembled with the histone variant H2A.Z. To understand the contribution of the transcription machinery in the disassembly of the +1 H2A.Z nucleosome, conditional mutants were used to block PIC assembly. A quantitative ChIP-seq approach, which allows detection of global occupancy change, was employed to measure H2A.Z occupancy. Blocking PIC assembly resulted in promoter-specific H2A.Z accumulation, indicating that the PIC is required to evict H2A.Z. By contrast, H2A.Z eviction was unaffected upon depletion of INO80, a remodeler previously reported to displace nucleosomal H2A.Z. Robust PIC-dependent H2A.Z eviction was observed at active and infrequently transcribed genes, indicating that constitutive H2A.Z turnover is a general phenomenon. Finally, sites with strong H2A.Z turnover precisely mark transcript starts, providing a new metric for identifying cryptic and alternative sites of initiation. DOI: http://dx.doi.org/10.7554/eLife.14243.001 PMID:27438412

  16. 50 CFR Table 8 to Part 679 - Harvest Zone Codes for Use With Vessel Activity Reports

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Harvest Zone Codes for Use With Vessel... ECONOMIC ZONE OFF ALASKA Pt. 679, Table 8 Table 8 to Part 679—Harvest Zone Codes for Use With Vessel Activity Reports Harvest Zone Description A1 BSAI EEZ off Alaska A2 GOA EEZ off Alaska B State waters...

  17. 6. Frisco Street streetscape, looking southeast. AZ167 and AZ168 (Archie ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Frisco Street streetscape, looking southeast. AZ-167 and AZ-168 (Archie Morrison Rental Houses) at left. - Clifton Townsite, Confluence of Chase Creek & San Francisco River, Clifton, Greenlee County, AZ

  18. XPS study of the surface chemistry on AZ31 and AZ91 magnesium alloys in dilute NaCl solution

    NASA Astrophysics Data System (ADS)

    Wang, Lei; Shinohara, Tadashi; Zhang, Bo-Ping

    2010-08-01

    The surface chemistry on AZ31 and AZ91 magnesium alloys was characterized by X-ray photoelectron spectroscopy (XPS) in the corrosion and the passivation zones. In the corrosion zone, the presence of Mg(OH) 2 and MgCO 3 species was found in the outer surface, whereas, in the inner layer, the co-existence of Mg(OH) 2, MgO and MgCO 3 species was observed for both alloys. The presence of Al 3+ in the surface electrolyte to form Al 2O 3/Al(OH) 3 and the formation of carbonate product provide a better passivation on the surfaces and retard the chloride-induced corrosion on the materials in the passivation zone.

  19. How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.

    PubMed

    Acuna, Claudio; Liu, Xinran; Südhof, Thomas C

    2016-08-17

    RIMs and RIM-binding proteins (RBPs) are evolutionary conserved multidomain proteins of presynaptic active zones that are known to recruit Ca(2+) channels; in addition, RIMs perform well-recognized functions in tethering and priming synaptic vesicles for exocytosis. However, deletions of RIMs or RBPs in mice cause only partial impairments in various active zone functions and have no effect on active zone structure, as visualized by electron micrographs, suggesting that their contribution to active zone functions is limited. Here, we show in synapses of the calyx of Held in vivo and hippocampal neurons in culture that combined, but not individual, deletions of RIMs and RBPs eliminate tethering and priming of synaptic vesicles, deplete presynaptic Ca(2+) channels, and ablate active zone complexes, as analyzed by electron microscopy of chemically fixed synapses. Thus, RBPs perform unexpectedly broad roles at the active zone that together with those of RIMs are essential for all active zone functions. PMID:27537484

  20. 78 FR 14963 - Foreign-Trade Zone 163-Ponce, Puerto Rico; Authorization of Production Activity; Zimmer...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... Foreign-Trade Zones Board Foreign-Trade Zone 163--Ponce, Puerto Rico; Authorization of Production Activity; Zimmer Manufacturing BV (Medical Devices); Ponce, Puerto Rico On November 1, 2012, CODEZOL, C.D., grantee of FTZ 163, submitted a notification of proposed production activity to the Foreign-Trade Zones...

  1. 33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Activities Far East Marine... SECURITY GENERAL COAST GUARD AREAS, DISTRICTS, SECTORS, MARINE INSPECTION ZONES, AND CAPTAIN OF THE PORT ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone....

  2. Spatial distribution of microfractures in damage zone along active faults

    NASA Astrophysics Data System (ADS)

    Mizoguchi, K.; Ueta, K.

    2011-12-01

    For basement faults without overlying quaternary sediments, there are few methods to determine whether the fault is active or not. Recently, we focus on microfracture characteristics of damage zone along active faults as used for the assessment of seismic activity of basement faults. In this study, we examined a newly-found active fault (Sasaki et al., 2011) located to the east of the epicentral area of 1943 Tottori earthquake, southwest Japan. The fault zone consists of the 75 cm thick fault core of the purple-colored clayey fault gouge and the fault breccia with cataclastic foliation, and the surrounding damage zone developed in Cretaceous Kyushozan granite. A subsidiary fault accompanying a fault core of white clayey fault gouge that ranges from 3 to 5 mm thickness is located at about 110 m from the main fault. We collected ten orientated samples 9 m to 180 m from the main fault. The samples were coated with epoxy and then thin sections were cut perpendicular to the fault plane and parallel to a horizontal plane because the slip direction is unknown. Microfracture density data were collected from 40 quartz grains per thin section (per sample). A thin section is marked with a square grid at 3 mm intervals and we picked one grain up in each square of the grid marked on the thin section to reduce operator sampling bias resulting from the selection of quartz grains. Quartz is suitable to estimate the damage that the rock sample has sustained because quartz without cleavage acts as an isotropic medium for fracturing and it is physically and chemically resistant to weathering than other minerals constituting the granite. We counted the number of microfractures that intersected a line which was drawn from the edge of each quartz grain, through the center point, to the other edge of the grain. The linear microfracture density for each sample is calculated to be the total number of microfractures intersecting the lines divided by the total counting line length. Under the

  3. Magnetic fields over active tectonic zones in ocean

    USGS Publications Warehouse

    Kopytenko, Yu. A.; Serebrianaya, P.M.; Nikitina, L.V.; Green, A.W.

    2002-01-01

    The aim of our work is to estimate the electromagnetic effects that can be detected in the submarine zones with hydrothermal activity. It is known that meso-scale flows appear in the regions over underwater volcanoes or hot rocks. Their origin is connected with heat flux and hot jets released from underwater volcanoes or faults in a sea bottom. Values of mean velocities and turbulent velocities in plumes were estimated. Quasiconstant magnetic fields induced by a hot jet and a vortex over a plume top are about 1-40 nT. Variable magnetic fields are about 0.1-1 nT. These magnetic disturbances in the sea medium create an additional natural electromagnetic background that must be considered when making detailed magnetic surveys. ?? 2002 Elsevier Science Ltd. All rights reserved.

  4. The possibility of a fuzzy zone of semiotic activity.

    PubMed

    Morioka, Masayoshi

    2007-12-01

    In this commentary I tried to further develop the idea of Madureira (Integr Psych Behav Sci, 42(2), 2007), who challenges to clarify the complex sexuality problem of homophobia from the viewpoint of the cultural semiotic activity. Two remarking points were proposed in this commentary article. First, I took notice of the boundary phenomenon constructed between the homophobia and the other. It has a cultural meaning. Concerning with this process, I introduced and examined the concept of tonus that is sensed a subtle changing process of tension in self-other relationship. The second, I discussed about the fuzzy zone of semiotic activity. If one can feel in oneself fuzzy awareness into the source of discomfort affect, it is able to be a creative moment in the tension of fuzzy field (A and non-A) where generates dialogical activity in both vertical and horizontal. Through this discussion, I proposed some remarks for the dissolution on the culturally constructed prejudice of sexuality. PMID:18232094

  5. 33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Activities Far East Marine... ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone. (a) Activities Far East's office is located in Yokota, Japan. The boundaries of Activities Far East's...

  6. 78 FR 28801 - Foreign-Trade Zone 117-Orange, TX, Authorization of Production Activity, Signal International...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ... notice in the Federal Register inviting public comment (78 FR 4383, 1-22-2013). The FTZ Board has... Foreign-Trade Zones Board Foreign-Trade Zone 117--Orange, TX, Authorization of Production Activity, Signal International Texas GP, LLC (Shipbuilding), Orange, TX On January 10, 2013, the Foreign Trade Zone of...

  7. 78 FR 4383 - Foreign-Trade Zone 117-Orange, Texas; Notification of Proposed Production Activity; Signal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 117--Orange, Texas; Notification of Proposed Production Activity; Signal International Texas GP, LLC (Shipbuilding), Orange, TX The Foreign Trade Zone of Southeast Texas, Inc., grantee of FTZ 117, submitted...

  8. 33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Activities Far East Marine Inspection Zone. 3.70-20 Section 3.70-20 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY GENERAL COAST GUARD AREAS, DISTRICTS, SECTORS, MARINE INSPECTION ZONES, AND CAPTAIN OF THE PORT ZONES Fourteenth Coast Guard District...

  9. Distributed Anelastic Strain and its Relationship to Compliant Zones Surrounding Active Faults of the Eastern California Shear Zone

    NASA Astrophysics Data System (ADS)

    Shelef, E.; Oskin, M.; Fialko, Y.

    2006-12-01

    Geologic measurements of distributed anelastic strain (DAS) adjacent to active strike slip faults of the Mojave Desert portion of the Eastern California shear zone quantify the magnitude, mechanism, temporal evolution, and relationship of DAS to fault compliant zones imaged via InSAR. Prefaulting markers (mylonitic lineation, dikes, and faults assumed linear prior to dextral faulting) in crystalline rocks next to the Harper Lake fault and Calico fault indicate that DAS accounts for 6 to 23 percent of total displacement and that this displacement scales with fault slip. We conclude that DAS is a significant, active process that is not restricted to the initial fault propagation stage. We find that the width of the zone of DAS is 400-700 m on each side of the faults studied, irrespective of total fault slip. 60 percent of the displacement due to DAS occurs within 100 m of the Calico fault. A similar zone of more intense deformation occurs adjacent to the Harper Lake fault. These 100m- wide-zones are of the same extent but much less intensely deformed compared to the damage zones surrounding the San Andreas fault. Based on these relationships, we hypothesize that damage feedback progressively focuses DAS into a stable, approximately 100-m-wide-zone where its intensity can increase proportionally to fault slip. Disruption of linear markers supports that DAS in crystalline rocks occurs via slip along secondary faults and small-scale block rotation with block sizes decreasing with proximity to faults. The widths of the geologically documented zones of DAS in the Eastern California shear zone are similar to the approximately 1 km width of compliant zones modeled from InSAR observations of surface deformation due to stress changes caused by nearby earthquakes. This correlation suggests a relationship between damage- reduction of shear modulus and displacement via DAS. Paleomagnetic measurements of prefaulting and syntectonically emplaced volcanic rocks in sedimentary

  10. DYNAMICS OF NASCENT AND ACTIVE ZONE ULTRASTRUCTURE AS SYNAPSES ENLARGE DURING LTP IN MATURE HIPPOCAMPUS

    PubMed Central

    Bell, Maria Elizabeth; Bourne, Jennifer N.; Chirillo, Michael A.; Mendenhall, John M.; Kuwajima, Masaaki; Harris, Kristen M.

    2014-01-01

    Nascent zones and active zones are adjacent synaptic regions that share a postsynaptic density, but nascent zones lack the presynaptic vesicles found at active zones. Here dendritic spine synapses were reconstructed through serial section electron microscopy (3DEM) and EM tomography to investigate nascent zone dynamics during long-term potentiation (LTP) in mature rat hippocampus. LTP was induced with theta-burst stimulation and comparisons were made to control stimulation in the same hippocampal slices at 5 minutes, 30 minutes, and 2 hours post-induction and to perfusion-fixed hippocampus in vivo. Nascent zones were present at the edges of ~35% of synapses in perfusion-fixed hippocampus and as many as ~50% of synapses in some hippocampal slice conditions. By 5 minutes, small dense core vesicles known to transport active zone proteins moved into more presynaptic boutons. By 30 minutes, nascent zone area decreased without significant change in synapse area, suggesting that presynaptic vesicles were recruited to pre-existing nascent zones. By 2 hours, both nascent and active zones were enlarged. Immunogold labeling revealed that glutamate receptors can be found in nascent zones; however, average distances from nascent zones to docked presynaptic vesicles ranged from 170±5 nm in perfusion-fixed hippocampus to 251±4 nm at enlarged synapses by 2 hours during LTP. Prior stochastic modeling suggests that falloff in glutamate concentration reduces the probability of glutamate receptor activation from 0.4 at the center of release to 0.1 just 200 nm away. Thus, conversion of nascent zones to functional active zones likely requires the recruitment of presynaptic vesicles during LTP. PMID:25043676

  11. Neuronal activity controls transsynaptic geometry.

    PubMed

    Glebov, Oleg O; Cox, Susan; Humphreys, Lawrence; Burrone, Juan

    2016-01-01

    The neuronal synapse is comprised of several distinct zones, including presynaptic vesicle zone (SVZ), active zone (AZ) and postsynaptic density (PSD). While correct relative positioning of these zones is believed to be essential for synaptic function, the mechanisms controlling their mutual localization remain unexplored. Here, we employ high-throughput quantitative confocal imaging, super-resolution and electron microscopy to visualize organization of synaptic subdomains in hippocampal neurons. Silencing of neuronal activity leads to reversible reorganization of the synaptic geometry, resulting in a increased overlap between immunostained AZ and PSD markers; in contrast, the SVZ-AZ spatial coupling is decreased. Bayesian blinking and bleaching (3B) reconstruction reveals that the distance between the AZ-PSD distance is decreased by 30 nm, while electron microscopy shows that the width of the synaptic cleft is decreased by 1.1 nm. Our findings show that multiple aspects of synaptic geometry are dynamically controlled by neuronal activity and suggest mutual repositioning of synaptic components as a potential novel mechanism contributing to the homeostatic forms of synaptic plasticity. PMID:26951792

  12. Neuronal activity controls transsynaptic geometry

    PubMed Central

    Glebov, Oleg O.; Cox, Susan; Humphreys, Lawrence; Burrone, Juan

    2016-01-01

    The neuronal synapse is comprised of several distinct zones, including presynaptic vesicle zone (SVZ), active zone (AZ) and postsynaptic density (PSD). While correct relative positioning of these zones is believed to be essential for synaptic function, the mechanisms controlling their mutual localization remain unexplored. Here, we employ high-throughput quantitative confocal imaging, super-resolution and electron microscopy to visualize organization of synaptic subdomains in hippocampal neurons. Silencing of neuronal activity leads to reversible reorganization of the synaptic geometry, resulting in a increased overlap between immunostained AZ and PSD markers; in contrast, the SVZ-AZ spatial coupling is decreased. Bayesian blinking and bleaching (3B) reconstruction reveals that the distance between the AZ-PSD distance is decreased by 30 nm, while electron microscopy shows that the width of the synaptic cleft is decreased by 1.1 nm. Our findings show that multiple aspects of synaptic geometry are dynamically controlled by neuronal activity and suggest mutual repositioning of synaptic components as a potential novel mechanism contributing to the homeostatic forms of synaptic plasticity. PMID:26951792

  13. Structure and seismic activity of the Lesser Antilles subduction zone

    NASA Astrophysics Data System (ADS)

    Evain, M.; Galve, A.; Charvis, P.; Laigle, M.; Ruiz Fernandez, M.; Kopp, H.; Hirn, A.; Flueh, E. R.; Thales Scientific Party

    2011-12-01

    Several active and passive seismic experiments conducted in 2007 in the framework of the European program "Thales Was Right" and of the French ANR program "Subsismanti" provided a unique set of geophysical data highlighting the deep structure of the central part of the Lesser Antilles subduction zone, offshore Dominica and Martinique, and its seismic activity during a period of 8 months. The region is characterized by a relatively low rate of seismicity that is often attributed to the slow (2 cm/yr) subduction of the old, 90 My, Atlantic lithosphere beneath the Caribbean Plate. Based on tomographic inversion of wide-angle seismic data, the forearc can clearly be divided into an inner forearc, characterised by a high vertical velocity gradient in the igneous crust, and an outer forearc with lower crustal velocity gradient. The thick, high velocity, inner forearc is possibly the extension at depth of the Mesozoic Caribbean crust outcropping in La Désirade Island. The outer forearc, up to 70 km wide in the northern part of the study area, is getting narrower to the south and disappears offshore Martinique. Based on its seismic velocity structure with velocities higher than 6 km/s the backstop consists, at least partly, of magmatic rocks. The outer forearc is also highly deformed and faulted within the subducting trend of the Tiburon Ridge. With respect to the inner forearc velocity structure the outer forearc basement could either correspond to an accreted oceanic terrane or made of highly fractured rocks. The inner forearc is a dense, poorly deformable crustal block, tilted southward as a whole. It acts as a rigid buttress increasing the strain within both the overriding and subducting plates. This appears clearly in the current local seismicity affecting the subducting and the overriding plates that is located beneath the inner forearc. We detected earthquakes beneath the Caribbean forearc and in the Atlantic oceanic plate as well. The main seismic activity is

  14. Contemporary approaches to studying and mapping of active water exchange zone of ground water

    NASA Astrophysics Data System (ADS)

    Moraru, C. Ye

    2016-03-01

    The article deals with a zone of ground water active exchange. New principles of the zone study and mapping under the platform hydrogeological condition are discussed. The assessment and distribution techniques are suggested for the active water exchange zone under the condition of hydrogeological parameterization uncertainty. The efficiency and significance of the suggested techniques are proved using the example of ground water in the southwest of Black Sea artesian basin.

  15. H2A.Z.1 Monoubiquitylation Antagonizes BRD2 to Maintain Poised Chromatin in ESCs.

    PubMed

    Surface, Lauren E; Fields, Paul A; Subramanian, Vidya; Behmer, Russell; Udeshi, Namrata; Peach, Sally E; Carr, Steven A; Jaffe, Jacob D; Boyer, Laurie A

    2016-02-01

    Histone variant H2A.Z occupies the promoters of active and poised, bivalent genes in embryonic stem cells (ESCs) to regulate developmental programs, yet how it contributes to these contrasting states is poorly understood. Here, we investigate the function of H2A.Z.1 monoubiquitylation (H2A.Z.1ub) by mutation of the PRC1 target residues (H2A.Z.1(K3R3)). We show that H2A.Z.1(K3R3) is properly incorporated at target promoters in murine ESCs (mESCs), but loss of monoubiquitylation leads to de-repression of bivalent genes, loss of Polycomb binding, and faulty lineage commitment. Using quantitative proteomics, we find that tandem bromodomain proteins, including the BET family member BRD2, are enriched in H2A.Z.1 chromatin. We further show that BRD2 is gained at de-repressed promoters in H2A.Z.1(K3R3) mESCs, whereas BRD2 inhibition restores gene silencing at these sites. Together, our study reveals an antagonistic relationship between H2A.Z.1ub and BRD2 to regulate the transcriptional balance at bivalent genes to enable proper execution of developmental programs. PMID:26804911

  16. 77 FR 68103 - Foreign-Trade Zone 163-Ponce, PR; Notification of Proposed Production Activity; Zimmer...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-15

    ... Foreign-Trade Zones Board Foreign-Trade Zone 163--Ponce, PR; Notification of Proposed Production Activity; Zimmer Manufacturing BV (Medical Devices); Ponce, PR CODEZOL, C.D., grantee of FTZ 163, submitted a notification of proposed production activity on behalf of Zimmer Manufacturing BV (Zimmer), located in...

  17. Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.

    PubMed

    Wang, Shan Shan H; Held, Richard G; Wong, Man Yan; Liu, Changliang; Karakhanyan, Aziz; Kaeser, Pascal S

    2016-08-17

    In a nerve terminal, synaptic vesicle docking and release are restricted to an active zone. The active zone is a protein scaffold that is attached to the presynaptic plasma membrane and opposed to postsynaptic receptors. Here, we generated conditional knockout mice removing the active zone proteins RIM and ELKS, which additionally led to loss of Munc13, Bassoon, Piccolo, and RIM-BP, indicating disassembly of the active zone. We observed a near-complete lack of synaptic vesicle docking and a strong reduction in vesicular release probability and the speed of exocytosis, but total vesicle numbers, SNARE protein levels, and postsynaptic densities remained unaffected. Despite loss of the priming proteins Munc13 and RIM and of docked vesicles, a pool of releasable vesicles remained. Thus, the active zone is necessary for synaptic vesicle docking and to enhance release probability, but releasable vesicles can be localized distant from the presynaptic plasma membrane. PMID:27537483

  18. MACROCALYX and JOINTLESS Interact in the Transcriptional Regulation of Tomato Fruit Abscission Zone Development1[C][W

    PubMed Central

    Nakano, Toshitsugu; Kimbara, Junji; Fujisawa, Masaki; Kitagawa, Mamiko; Ihashi, Nao; Maeda, Hideo; Kasumi, Takafumi; Ito, Yasuhiro

    2012-01-01

    Abscission in plants is a crucial process used to shed organs such as leaves, flowers, and fruits when they are senescent, damaged, or mature. Abscission occurs at predetermined positions called abscission zones (AZs). Although the regulation of fruit abscission is essential for agriculture, the developmental mechanisms remain unclear. Here, we describe a novel transcription factor regulating the development of tomato (Solanum lycopersicum) pedicel AZs. We found that the development of tomato pedicel AZs requires the gene MACROCALYX (MC), which was previously identified as a sepal size regulator and encodes a MADS-box transcription factor. MC has significant sequence similarity to Arabidopsis (Arabidopsis thaliana) FRUITFULL, which is involved in the regulation of fruit dehiscent zone development. The MC protein interacted physically with another MADS-box protein, JOINTLESS, which is known as a regulator of fruit abscission; the resulting heterodimer acquired a specific DNA-binding activity. Transcriptome analyses of pedicels at the preabscission stage revealed that the expression of the genes involved in phytohormone-related functions, cell wall modifications, fatty acid metabolism, and transcription factors is regulated by MC and JOINTLESS. The regulated genes include homologs of Arabidopsis WUSCHEL, REGULATOR OF AXILLARY MERISTEMS, CUP-SHAPED COTYLEDON, and LATERAL SUPPRESSOR. These Arabidopsis genes encode well-characterized transcription factors regulating meristem maintenance, axillary meristem development, and boundary formation in plant tissues. The tomato homologs were specifically expressed in AZs but not in other pedicel tissues, suggesting that these transcription factors may play key roles in pedicel AZ development. PMID:22106095

  19. Development and Validation of a Model to Predict Aerosol Breathing Zone Concentrations During Common Outdoor Activities

    EPA Science Inventory

    Research has been conducted on aerosol emission rates during various activities as well as aerosol transport into the breathing zone under idealized conditions. However, there has been little effort to link the two into a model for predicting a person’s breathing zone concentrat...

  20. 78 FR 68026 - Foreign-Trade Zone (FTZ) 99-Wilmington, Delaware, Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone (FTZ) 99--Wilmington, Delaware, Notification of Proposed Production Activity, Noramco, Inc., (Pharmaceutical Intermediate), Wilmington, Delaware The Delaware Economic Development Office, grantee of FTZ...

  1. 77 FR 36997 - Foreign-Trade Zone 7-Mayaguez, PR; Notification of Proposed Production Activity; Baxter...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 7--Mayaguez, PR; Notification of Proposed Production Activity; Baxter Healthcare of Puerto Rico; (Pharmaceutical and Nutritional Intravenous Bags and Administration Sets); Aibonito and Jayuya, PR The...

  2. 77 FR 61381 - Foreign-Trade Zone 7-Mayaguez, Puerto Rico, Authorization of Production Activity, Baxter...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-09

    ... Foreign-Trade Zones Board Foreign-Trade Zone 7--Mayaguez, Puerto Rico, Authorization of Production Activity, Baxter Healthcare of Puerto Rico, (Pharmaceutical and Nutritional Intravenous Bags and Administration Sets); Aibonito and Jayuya, Puerto Rico The Puerto Rico Industrial Development Company, grantee...

  3. 77 FR 48127 - Foreign-Trade Zone 20-Suffolk, VA; Notification of Proposed Production Activity, Usui...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 20--Suffolk, VA; Notification of Proposed Production Activity, Usui International Corporation, (Diesel Engine Fuel Lines), Chesapeake, VA The Virginia Port Authority, grantee of FTZ 20, submitted a...

  4. 15 CFR 400.49 - Monitoring and reviews of zone operations and activity.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Monitoring and reviews of zone operations and activity. 400.49 Section 400.49 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF...

  5. 15 CFR 400.49 - Monitoring and reviews of zone operations and activity.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Monitoring and reviews of zone operations and activity. 400.49 Section 400.49 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF...

  6. 77 FR 74170 - Foreign-Trade Zone 84-Houston, TX; Notification of Proposed Production Activity; Mitsubishi...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 84--Houston, TX; Notification of Proposed Production Activity; Mitsubishi Caterpillar Forklift America Inc.; (Forklift Trucks); Houston, TX The Port of Houston Authority, grantee of FTZ 84, submitted...

  7. 77 FR 58354 - Foreign-Trade Zone 265-Conroe, TX; Notification of Proposed Production Activity, Bauer...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 265--Conroe, TX; Notification of Proposed Production Activity, Bauer Manufacturing Inc. (Pile Drivers and Boring Machinery); Conroe, TX The City of Conroe, Texas, grantee of FTZ 265, submitted a notification...

  8. 77 FR 55182 - Foreign-Trade Zone 45-Portland, OR, Authorization of Production Activity, Shimadzu USA...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-07

    ...-52-2012, 77 FR 48127, 8/13/2012). The notification was processed in accordance with the regulations... (77 FR 28353, 5/14/2012). The FTZ Board has determined that no further review of the activity is... Foreign-Trade Zones Board Foreign-Trade Zone 45--Portland, OR, Authorization of Production...

  9. 77 FR 71167 - Foreign-Trade Zone 59-Lincoln, Nebraska, Authorization of Production Activity, Novartis Consumer...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-29

    ... inviting public comment (77 FR 50462, August 21, 2012). The FTZ Board has determined that no further review... Foreign-Trade Zones Board Foreign-Trade Zone 59--Lincoln, Nebraska, Authorization of Production Activity, Novartis Consumer Health, Inc. (Pharmaceutical and Related Preparations Production), Lincoln,...

  10. 78 FR 66330 - Foreign-Trade Zone 196-Fort Worth, Texas, Authorization of Production Activity, Flextronics...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 196--Fort Worth, Texas, Authorization of Production Activity, Flextronics International USA, Inc. (Mobile Phone Assembly and Kitting), Fort Worth, Texas On June 14, 2013, Flextronics International USA,...

  11. Earthquake mechanisms and active tectonics of the Hellenic subduction zone

    NASA Astrophysics Data System (ADS)

    Shaw, Beth; Jackson, James

    2010-05-01

    We use improved focal mechanisms and centroid depth estimates of earthquakes, combined with GPS velocities, to examine the tectonics of the Hellenic subduction zone, and in particular the processes occurring at both ends of the Hellenic Arc. Nubia-Aegean convergence is accommodated by shallowly dipping thrust-faulting along the subduction-zone interface, as well as by steeper splay faults in the overriding material. From a comparison of observed and expected seismic moment release over the last 100 yr, combined with existing knowledge of the longer-term documented historical record, we confirm earlier suggestions that most (80 per cent) of this convergence is accommodated aseismically, that is, that the subduction zone is uncoupled. This conclusion is robust, even allowing for rare very large earthquakes on splay faults, such as that of AD 365, and also allowing for the contribution of small earthquakes. The downgoing Nubian plate deforms by arc-parallel contraction at all depths, from 200 km seaward of Crete to at least 100 km within the subducting slab. Extensional (T) axes of earthquakes are aligned downdip within the descending slab suggesting that, even if the aseismic prolongation of the slab has reached the 670 km mantle discontinuity, it does not transmit stresses to shallower depths. Shallow thrust-faulting earthquakes on the subduction interface show a divergence of slip vectors round the arc, and GPS measurements show that this is accommodated mainly by E-W extension on normal faults in the overriding Aegean material. The eastern end of the subduction zone, south of Rhodes, displays distributed deformation in the overriding material, including a mixture of strike-slip and splay-thrust faulting, and probably involves rotations about a vertical axes. Here slip on the interface itself is by thrust faulting with slip vectors oblique to the arc but parallel to the overall Nubia-Aegean convergence: there is no evidence for slip-partitioning in the traditional

  12. [Differences of activations in visual and associative zones during figurative and verbal activity].

    PubMed

    Nagornova, Zh V; Shemiakina, N V

    2014-04-01

    The study considers correlates of figurative and verbal tasks performance during attention paid to visual stimuli. There are 34 subjects (20 female, mean age 21, 2.5 [SD]) took parts in the study. During subjects performance of the task, there was carried out EEG registration from 19 sites according to 10-20%. Performance of the figurative creative task in comparison with control non-creative task of the same modality was accompanied by activation of occipital and parietal zones of the cerebral cortex (decrease of EEG spectral power in alpha 1 (7.5-9.5 Hz) and alpha2 (10-12.5 Hz) frequency bands was observed) whereas performance of a verbal creative task in the similar test-control comparison was accompanied by decrease of activation in occipital zones (revealed through increase of EEG spectral in alphal and alpha2 frequency bands). As visual stimuli were shown during the whole time of the creative and control tasks fulfilment was made an assumption observed distinction can be connected with redistribution of attention focus at various types of creative activity (figurative or verbal). PMID:25272453

  13. FINAL REPORT. CONTROL OF BIOLOGICALLY ACTIVE DEGRADATION ZONES BY VERTICAL HETEROGENEITY: APPLICATIONS IN FRACTURED MEDIA

    EPA Science Inventory

    The key objective of this research was to determine the distribution of biologically active contaminant degradation zones in a fractured, subsurface medium with respect to vertical heterogeneities. Our expectation was that
    hydrogeological properties would determine the size, d...

  14. Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function

    PubMed Central

    Muhammad, Karzan; Reddy-Alla, Suneel; Driller, Jan H; Schreiner, Dietmar; Rey, Ulises; Böhme, Mathias A.; Hollmann, Christina; Ramesh, Niraja; Depner, Harald; Lützkendorf, Janine; Matkovic, Tanja; Götz, Torsten; Bergeron, Dominique D.; Schmoranzer, Jan; Goettfert, Fabian; Holt, Mathew; Wahl, Markus C.; Hell, Stefan W.; Scheiffele, Peter; Walter, Alexander M.; Loll, Bernhard; Sigrist, Stephan J.

    2015-01-01

    Assembly and maturation of synapses at the Drosophila neuromuscular junction (NMJ) depend on trans-synaptic neurexin/neuroligin signalling, which is promoted by the scaffolding protein Syd-1 binding to neurexin. Here we report that the scaffold protein spinophilin binds to the C-terminal portion of neurexin and is needed to limit neurexin/neuroligin signalling by acting antagonistic to Syd-1. Loss of presynaptic spinophilin results in the formation of excess, but atypically small active zones. Neuroligin-1/neurexin-1/Syd-1 levels are increased at spinophilin mutant NMJs, and removal of single copies of the neurexin-1, Syd-1 or neuroligin-1 genes suppresses the spinophilin-active zone phenotype. Evoked transmission is strongly reduced at spinophilin terminals, owing to a severely reduced release probability at individual active zones. We conclude that presynaptic spinophilin fine-tunes neurexin/neuroligin signalling to control active zone number and functionality, thereby optimizing them for action potential-induced exocytosis. PMID:26471740

  15. Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function.

    PubMed

    Muhammad, Karzan; Reddy-Alla, Suneel; Driller, Jan H; Schreiner, Dietmar; Rey, Ulises; Böhme, Mathias A; Hollmann, Christina; Ramesh, Niraja; Depner, Harald; Lützkendorf, Janine; Matkovic, Tanja; Götz, Torsten; Bergeron, Dominique D; Schmoranzer, Jan; Goettfert, Fabian; Holt, Mathew; Wahl, Markus C; Hell, Stefan W; Scheiffele, Peter; Walter, Alexander M; Loll, Bernhard; Sigrist, Stephan J

    2015-01-01

    Assembly and maturation of synapses at the Drosophila neuromuscular junction (NMJ) depend on trans-synaptic neurexin/neuroligin signalling, which is promoted by the scaffolding protein Syd-1 binding to neurexin. Here we report that the scaffold protein spinophilin binds to the C-terminal portion of neurexin and is needed to limit neurexin/neuroligin signalling by acting antagonistic to Syd-1. Loss of presynaptic spinophilin results in the formation of excess, but atypically small active zones. Neuroligin-1/neurexin-1/Syd-1 levels are increased at spinophilin mutant NMJs, and removal of single copies of the neurexin-1, Syd-1 or neuroligin-1 genes suppresses the spinophilin-active zone phenotype. Evoked transmission is strongly reduced at spinophilin terminals, owing to a severely reduced release probability at individual active zones. We conclude that presynaptic spinophilin fine-tunes neurexin/neuroligin signalling to control active zone number and functionality, thereby optimizing them for action potential-induced exocytosis. PMID:26471740

  16. Group Problem Solving as a Zone of Proximal Development activity

    NASA Astrophysics Data System (ADS)

    Brewe, Eric

    2006-12-01

    Vygotsky described learning as a process, intertwined with development, which is strongly influenced by social interactions with others that are at differing developmental stages.i These interactions create a Zone of Proximal Development for each member of the interaction. Vygotsky’s notion of social constructivism is not only a theory of learning, but also of development. While teaching introductory physics in an interactive format, I have found manifestations of Vygotsky’s theory in my classroom. The source of evidence is a paired problem solution. A standard mechanics problem was solved by students in two classes as a homework assignment. Students handed in the homework and then solved the same problem in small groups. The solutions to both the group and individual problem were assessed by multiple reviewers. In many cases the group score was the same as the highest individual score in the group, but in some cases, the group score was higher than any individual score. For this poster, I will analyze the individual and group scores and focus on three groups solutions and video that provide evidence of learning through membership in a Zone of Proximal Development. Endnotes i L. Vygotsky -Mind and society: The development of higher mental processes. Cambridge, MA: Harvard University Press. (1978).

  17. The AzTEC Mathematics Project (AMP).

    ERIC Educational Resources Information Center

    Johnson, Gae R.

    The AzTEC Mathematics Project (AMP) is a statewide partnership among Arizona's Regents universities and state community colleges, partner school districts, and economic communities. AzTec is committed to preparing highly qualified K-12 mathematics and science teachers. AMP targeted Native American teachers and teachers of Native American students…

  18. Meteor Crater, AZ

    NASA Technical Reports Server (NTRS)

    2002-01-01

    -term research and technology program designed to examine Earth's land, oceans, atmosphere, ice and life as a total integrated system.

    The broad spectral coverage and high spectral resolution of ASTER will provide scientists in numerous disciplines with critical information for surface mapping, and monitoring dynamic conditions and temporal change. Example applications are: monitoring glacial advances and retreats; monitoring potentially active volcanoes; identifying crop stress; determining cloud morphology and physical properties; wetlands evaluation; thermal pollution monitoring; coral reef degradation; surface temperature mapping of soils and geology; and measuring surface heat balance.

    Size: 15 x 30 km (9 x 18 miles) Location: 35.1 deg. North lat., 111.0 deg. West long. Orientation: Northeast at top Image Data: ASTER bands 1,2, and 3. Original Data Resolution: 15 m Date Acquired: May 17, 2001

  19. The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis.

    PubMed

    McKim, Sarah M; Stenvik, Grethe-Elisabeth; Butenko, Melinka A; Kristiansen, Wenche; Cho, Sung Ki; Hepworth, Shelley R; Aalen, Reidunn B; Haughn, George W

    2008-04-01

    The Arabidopsis BLADE-ON-PETIOLE 1 (BOP1) and BOP2 genes encode redundant transcription factors that promote morphological asymmetry during leaf and floral development. Loss-of-function bop1 bop2 mutants display a range of developmental defects, including a loss of floral organ abscission. Abscission occurs along specialised cell files, called abscission zones (AZs) that develop at the junction between the leaving organ and main plant body. We have characterized the bop1 bop2 abscission phenotype to determine how BOP1 and BOP2 contribute to the known abscission developmental framework. Histological analysis and petal breakstrength measurements of bop1 bop2 flowers show no differentiation of floral AZs. Furthermore, vestigial cauline leaf AZs are also undifferentiated in bop1 bop2 mutants, suggesting that BOP proteins are essential to establish AZ cells in different tissues. In support of this hypothesis, BOP1/BOP2 activity is required for both premature floral organ abscission and the ectopic abscission of cauline leaves promoted by the INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) gene under the control of the constitutive CaMV 35S promoter. Expression of several abscission-related marker genes, including IDA, is relatively unperturbed in bop1 bop2 mutants, indicating that these AZ genes respond to positional cues that are independent of BOP1/BOP2 activity. We also show that BOP1 and BOP2 promote growth of nectary glands, which normally develop at the receptacle adjacent to developing AZs. Taken together, these data suggest that BOP1/BOP2 activity is required for multiple cell differentiation events in the proximal regions of inflorescence lateral organs. PMID:18339677

  20. Correlation between the surface chemistry and the atmospheric corrosion of AZ31, AZ80 and AZ91D magnesium alloys

    NASA Astrophysics Data System (ADS)

    Feliu, S., Jr.; Pardo, A.; Merino, M. C.; Coy, A. E.; Viejo, F.; Arrabal, R.

    2009-01-01

    X-ray photoelectron spectroscopy (XPS) was used in order to investigate the correlation between the surface chemistry and the atmospheric corrosion of AZ31, AZ80 and AZ91D magnesium alloys exposed to 98% relative humidity at 50 °C. Commercially pure magnesium, used as the reference material, revealed MgO, Mg(OH) 2 and tracers of magnesium carbonate in the air-formed film. For the AZ80 and AZ91D alloys, the amount of magnesium carbonate formed on the surface reached similar values to those of MgO and Mg(OH) 2. A linear relation between the amount of magnesium carbonate formed on the surface and the subsequent corrosion behaviour in the humid environment was found. The AZ80 alloy revealed the highest amount of magnesium carbonate in the air-formed film and the highest atmospheric corrosion resistance, even higher than the AZ91D alloy, indicating that aluminium distribution in the alloy microstructure influenced the amount of magnesium carbonate formed.

  1. Geomorphic Indices in the Assessment of Tectonic Activity in Forearc of the Active Mexican Subduction Zone

    NASA Astrophysics Data System (ADS)

    Gaidzik, K.; Ramirez-Herrera, M. T.

    2015-12-01

    Rapid development of GIS techniques and constant advancement of digital elevation models significantly improved the accuracy of extraction of information on active tectonics from landscape features. Numerous attempts were made to quantitatively evaluate recent tectonic activity using GIS and DEMs, and a set of geomorphic indices (GI), however these studies focused mainly on sub-basins or small-scale areal units. In forearc regions where crustal deformation is usually large-scale and do not concentrate only along one specific fault, an assessment of the complete basin is more accurate. We present here the first attempt to implement thirteen GI in the assessment of active tectonics of a forearc region of an active convergent margin using the entire river basins. The GIs were divided into groups: BTAI - basin geomorphic indices (reflecting areal erosion vs. tectonics) and STAI - stream geomorphic indices (reflecting vertical erosion vs. tectonics). We calculated selected indices for 9 large (> 450 km2) drainage basins. Then we categorized the obtained results of each index into three classes of relative tectonic activity: 1 - high, 2 - moderate, and 3 - low. Finally we averaged these classes for each basin to determine the tectonic activity level (TAI). The analysis for the case study area, the Guerrero sector at the Mexican subduction zone, revealed high tectonic activity in this area, particularly in its central and, to a lesser degree, eastern part. This pattern agrees with and is supported by interpretation of satellite images and DEM, and field observations. The results proved that the proposed approach indeed allows identification and recognition of areas witnessing recent tectonic deformation. Moreover, our results indicated that, even though no large earthquake has been recorded in this sector for more than 100 years, the area is highly active and may represent a seismic hazard for the region.

  2. Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells

    PubMed Central

    Sundaresan, Srivignesh; Philosoph-Hadas, Sonia; Riov, Joseph; Belausov, Eduard; Kochanek, Betina; Tucker, Mark L.; Meir, Shimon

    2015-01-01

    In vivo changes in the cytosolic pH of abscission zone (AZ) cells were visualized using confocal microscopic detection of the fluorescent pH-sensitive and intracellularly trapped dye, 2’,7’-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), driven by its acetoxymethyl ester. A specific and gradual increase in the cytosolic pH of AZ cells was observed during natural abscission of flower organs in Arabidopsis thaliana and wild rocket (Diplotaxis tenuifolia), and during flower pedicel abscission induced by flower removal in tomato (Solanum lycopersicum Mill). The alkalization pattern in the first two species paralleled the acceleration or inhibition of flower organ abscission induced by ethylene or its inhibitor 1-methylcyclopropene (1-MCP), respectively. Similarly, 1-MCP pre-treatment of tomato inflorescence explants abolished the pH increase in AZ cells and pedicel abscission induced by flower removal. Examination of the pH changes in the AZ cells of Arabidopsis mutants defective in both ethylene-induced (ctr1, ein2, eto4) and ethylene-independent (ida, nev7, dab5) abscission pathways confirmed these results. The data indicate that the pH changes in the AZ cells are part of both the ethylene-sensitive and -insensitive abscission pathways, and occur concomitantly with the execution of organ abscission. pH can affect enzymatic activities and/or act as a signal for gene expression. Changes in pH during abscission could occur via regulation of transporters in AZ cells, which might affect cytosolic pH. Indeed, four genes associated with pH regulation, vacuolar H+-ATPase, putative high-affinity nitrate transporter, and two GTP-binding proteins, were specifically up-regulated in tomato flower AZ following abscission induction, and 1-MCP reduced or abolished the increased expression. PMID:25504336

  3. Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells.

    PubMed

    Sundaresan, Srivignesh; Philosoph-Hadas, Sonia; Riov, Joseph; Belausov, Eduard; Kochanek, Betina; Tucker, Mark L; Meir, Shimon

    2015-03-01

    In vivo changes in the cytosolic pH of abscission zone (AZ) cells were visualized using confocal microscopic detection of the fluorescent pH-sensitive and intracellularly trapped dye, 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), driven by its acetoxymethyl ester. A specific and gradual increase in the cytosolic pH of AZ cells was observed during natural abscission of flower organs in Arabidopsis thaliana and wild rocket (Diplotaxis tenuifolia), and during flower pedicel abscission induced by flower removal in tomato (Solanum lycopersicum Mill). The alkalization pattern in the first two species paralleled the acceleration or inhibition of flower organ abscission induced by ethylene or its inhibitor 1-methylcyclopropene (1-MCP), respectively. Similarly, 1-MCP pre-treatment of tomato inflorescence explants abolished the pH increase in AZ cells and pedicel abscission induced by flower removal. Examination of the pH changes in the AZ cells of Arabidopsis mutants defective in both ethylene-induced (ctr1, ein2, eto4) and ethylene-independent (ida, nev7, dab5) abscission pathways confirmed these results. The data indicate that the pH changes in the AZ cells are part of both the ethylene-sensitive and -insensitive abscission pathways, and occur concomitantly with the execution of organ abscission. pH can affect enzymatic activities and/or act as a signal for gene expression. Changes in pH during abscission could occur via regulation of transporters in AZ cells, which might affect cytosolic pH. Indeed, four genes associated with pH regulation, vacuolar H(+)-ATPase, putative high-affinity nitrate transporter, and two GTP-binding proteins, were specifically up-regulated in tomato flower AZ following abscission induction, and 1-MCP reduced or abolished the increased expression. PMID:25504336

  4. The Root Apex of Arabidopsis thaliana Consists of Four Distinct Zones of Growth Activities

    PubMed Central

    De Cnodder, Tinne; Le, Jie

    2006-01-01

    In the growing apex of Arabidopsis thaliana primary roots, cells proceed through four distinct phases of cellular activities. These zones and their boundaries can be well defined based on their characteristic cellular activities. The meristematic zone comprises, and is limited to, all cells that undergo mitotic divisions. Detailed in vivo analysis of transgenic lines reveals that, in the Columbia-0 ecotype, the meristem stretches up to 200 µm away from the junction between root and root cap (RCJ). In the transition zone, 200 to about 520 µm away from the RCJ, cells undergo physiological changes as they prepare for their fast elongation. Upon entering the transition zone, they progressively develop a central vacuole, polarize the cytoskeleton and remodel their cell walls. Cells grow slowly during this transition: it takes ten hours to triplicate cell length from 8.5 to about 35 µm in the trichoblast cell files. In the fast elongation zone, which covers the zone from 520 to about 850 µm from the RCJ, cell length quadruplicates to about 140 µm in only two hours. This is accompanied by drastic and specific cell wall alterations. Finally, root hairs fully develop in the growth terminating zone, where root cells undergo a minor elongation to reach their mature lengths. PMID:19517000

  5. 78 FR 75331 - Foreign-Trade Zone (FTZ) 100-Dayton, Ohio; Notification of Proposed Production Activity; THOR...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-11

    ... Foreign-Trade Zones Board Foreign-Trade Zone (FTZ) 100--Dayton, Ohio; Notification of Proposed Production Activity; THOR Industries, Inc. (Commercial Bus Manufacturing); Jackson Center, Ohio The Greater Dayton Foreign-Trade Zone, Inc., grantee of FTZ 100, submitted a notification of proposed production activity...

  6. The Histone Chaperones FACT and Spt6 Restrict H2A.Z from Intragenic Locations

    PubMed Central

    Jeronimo, Célia; Watanabe, Shinya; Kaplan, Craig D.; Peterson, Craig L.; Robert, François

    2015-01-01

    SUMMARY H2A.Z is a highly conserved histone variant involved in several key nuclear processes. It is incorporated into promoters by SWR-C-related chromatin remodeling complexes, but whether it is also actively excluded from non-promoter regions is not clear. Here, we provide genomic and biochemical evidence that RNA polymerase II (RNAPII) elongation-associated histone chaperones FACT and Spt6 both contribute to restricting H2A.Z from intragenic regions. In the absence of FACT or Spt6, the lack of efficient nucleosome reassembly coupled to pervasive incorporation of H2A.Z by mislocalized SWR-C alters chromatin composition and contributes to cryptic initiation. Thus, chaperone-mediated H2A.Z confinement is crucial for restricting the chromatin signature of gene promoters, which otherwise may license or promote cryptic transcription. PMID:25959393

  7. Triplet supercurrent due to spin-active zones in a Josephson junction

    NASA Astrophysics Data System (ADS)

    Linder, Jacob; Sudbø, Asle

    2010-07-01

    Motivated by a recent experiment evidencing triplet superconductivity in a ferromagnetic Josephson junction with a Cu2MnAl -Heusler barrier, we construct a theoretical model accounting for this observation. The key ingredients in our model which generate the triplet supercurrent are spin-active zones, characterized by an effective canted interface magnetic moment. Using a numerical solution of the quasiclassical equations of superconductivity with spin-active boundary conditions, we find qualitatively very good agreement with the experimentally observed supercurrent. Further experimental implications of the spin-active zones are discussed.

  8. System Description for Tank 241-AZ-101 Waste Retrieval Data Acquisition System

    SciTech Connect

    ROMERO, S.G.

    2000-02-14

    The proposed activity provides the description of the Data Acquisition System for Tank 241-AZ-101. This description is documented in HNF-5572, Tank 241-AZ-101 Waste Retrieval Data Acquisition System (DAS). This activity supports the planned mixer pump tests for Tank 241-AZ-101. Tank 241-AZ-101 has been selected for the first full-scale demonstration of a mixer pump system. The tank currently holds over 960,000 gallons of neutralized current acid waste, including approximately 12.7 inches of settling solids (sludge) at the bottom of the tank. As described in Addendum 4 of the FSAR (LMHC 2000a), two 300 HP mixer pumps with associated measurement and monitoring equipment have been installed in Tank 241-AZ-101. The purpose of the Tank 241-AZ-101 retrieval system Data Acquisition System (DAS) is to provide monitoring and data acquisition of key parameters in order to confirm the effectiveness of the mixer pumps utilized for suspending solids in the tank. The suspension of solids in Tank 241-AZ-101 is necessary for pretreatment of the neutralized current acid waste and eventual disposal as glass via the Hanford Waste Vitrification Plant. HNF-5572 provides a basic description of the Tank 241-AZ-101 retrieval system DAS, including the field instrumentation and application software. The DAS is provided to fulfill requirements for data collection and monitoring. This document is not an operations procedure or is it intended to describe the mixing operation. This USQ screening provides evaluation of HNF-5572 (Revision 1) including the changes as documented on ECN 654001. The changes include (1) add information on historical trending and data backup, (2) modify DAS I/O list in Appendix E to reflect actual conditions in the field, and (3) delete IP address in Appendix F per Lockheed Martin Services, Inc. request.

  9. Calcium-channel number critically influences synaptic strength and plasticity at the active zone

    PubMed Central

    Sheng, Jiansong; He, Liming; Zheng, Hongwei; Xue, Lei; Luo, Fujun; Shin, Wonchul; Sun, Tao; Kuner, Thomas; Yue, David T; Wu, Ling-Gang

    2016-01-01

    How synaptic-vesicle release is controlled at the basic release structure, the active zone, is poorly understood. By performing cell-attached current and capacitance recordings predominantly at single active zones in rat calyces, we found that single active zones contained 5-218 (mean, 42) calcium channels and 1–10 (mean, 5) readily releasable vesicles (RRVs) and released 0–5 vesicles during a 2-ms depolarization. Large variation in the number of calcium channels caused wide variation in release strength (measured during a 2-ms depolarization) by regulating the RRV release probability (PRRV) and the RRV number. Consequently, an action potential opened ~1–35 (mean, ~7) channels, resulting in different release probabilities at different active zones. As the number of calcium-channels determined PRRV, it critically influenced whether subsequent release would be facilitated or depressed. Regulating calcium channel density at active zones may thus be a major mechanism to yield synapses with different release properties and plasticity. These findings may explain large differences reported at synapses regarding release strength (release of 0, 1 or multiple vesicles), PRRV, short-term plasticity, calcium transients and the requisite calcium-channel number for triggering release. PMID:22683682

  10. 77 FR 19927 - Amendment of Class E Airspace; Willcox, AZ, and Revocation of Class E Airspace; Cochise, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-03

    ...This action modifies Class E airspace at Willcox, AZ, and removes Class E airspace at Cochise, AZ. The airspace designation listed as Cochise, AZ, is combined with Cochise County Airport, Willcox, AZ. Controlled airspace is necessary to accommodate aircraft using Area Navigation (RNAV) Global Positioning System (GPS) standard instrument approach procedures at Cochise County Airport, Willcox,......

  11. Low resistivity and permeability in actively deforming shear zones on the San Andreas Fault at SAFOD

    NASA Astrophysics Data System (ADS)

    Morrow, C.; Lockner, D. A.; Hickman, S.

    2015-12-01

    The San Andreas Fault Observatory at Depth (SAFOD) scientific drill hole near Parkfield, California, crosses the San Andreas Fault at a depth of 2.7 km. Downhole measurements and analysis of core retrieved from Phase 3 drilling reveal two narrow, actively deforming zones of smectite-clay gouge within a roughly 200 m wide fault damage zone of sandstones, siltstones, and mudstones. Here we report electrical resistivity and permeability measurements on core samples from all of these structural units at effective confining pressures up to 120 MPa. Electrical resistivity (~10 Ω-m) and permeability (10-21 to 10-22 m2) in the actively deforming zones were 1 to 2 orders of magnitude lower than the surrounding damage zone material, consistent with broader-scale observations from the downhole resistivity and seismic velocity logs. The higher porosity of the clay gouge, 2 to 8 times greater than that in the damage zone rocks, along with surface conduction were the principal factors contributing to the observed low resistivities. The high percentage of fine-grained clay in the deforming zones also greatly reduced permeability to values low enough to create a barrier to fluid flow across the fault. Together, resistivity and permeability data can be used to assess the hydrogeologic characteristics of the fault, key to understanding fault structure and strength. The low resistivities and strength measurements of the SAFOD core are consistent with observations of low resistivity clays that are often found in the principal slip zones of other active faults making resistivity logs a valuable tool for identifying these zones.

  12. H2A.Z.1 mono-ubiquitylation antagonizes BRD2 to maintain poised chromatin in ESCs

    PubMed Central

    Surface, Lauren E.; Fields, Paul A.; Subramanian, Vidya; Behmer, Russell; Udeshi, Namrata; Peach, Sally E.; Jaffe, Jacob D.; Boyer, Laurie A.

    2016-01-01

    SUMMARY Histone variant H2A.Z occupies the promoters of active and poised, bivalent genes in ESCs to regulate developmental programs, yet how it contributes to these contrasting states is poorly understood. Here, we investigate the function of H2A.Z.1 mono-ubiquitylation (H2A.Z.1ub) by mutation of the PRC1 target residues (H2A.Z.1K3R3). We show that H2A.Z.1K3R3 is properly incorporated at target promoters in murine ESCs (mESCs), however, loss of mono-ubiquitylation leads to de-repression of bivalent genes, loss of Polycomb binding, and to faulty lineage commitment. Using quantitative proteomics, we find that tandem bromodomain proteins, including the BET family member Brd2, are enriched in H2A.Z.1 chromatin. We further show that Brd2 is gained at de-repressed promoters in H2A.Z.1K3R3 mESCs whereas Brd2 inhibition restores gene silencing at these sites. Together, our study reveals an antagonistic relationship between H2A.Z.1ub and Brd2 to regulate the transcriptional balance at bivalent genes to enable proper execution of developmental programs. PMID:26804911

  13. 20. Streetscape, South Coronado Boulevard, looking southwest Righttoleft, AZ198(Margaret B. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Streetscape, South Coronado Boulevard, looking southwest Right-to-left, AZ-198(Margaret B. Hill House), AZ-199 (Henry H. Hill House), AZ-200 (Henry H. Hill Rental House), AZ-201 (Otto Rosenthal House), AZ-202 (J. M. Stevens House), AZ-203 (L J. Dunagan House), AZ204 (C. W. Beck House), and AZ-205 (F. D. Connor House) face the street - Clifton Townsite, Confluence of Chase Creek & San Francisco River, Clifton, Greenlee County, AZ

  14. Structural and Lithologic Characteristics of the Wenchuan Earthquake Fault Zone and its Relationship with Seismic Activity

    NASA Astrophysics Data System (ADS)

    Wang, H.; Li, H.; Pei, J.; Li, T.; Huang, Y.; Zhao, Z.

    2010-12-01

    the older earthquake, but rather along the edge of the gouge. According to the gouge statistics of the whole fault zone, seismic events have the obvious tendency towards the foot wall, and the thickness of gouge is proportional to the activity of the fault, indicating that the width of fault zone is directly related to the number and evolution history of earthquakes . Repeated earthquakes maybe the main cause for the formation of the Longmenshan Moutains

  15. Expression of P. falciparum var Genes Involves Exchange of the Histone Variant H2A.Z at the Promoter

    PubMed Central

    Petter, Michaela; Lee, Chin Chin; Byrne, Timothy J.; Boysen, Katja E.; Volz, Jennifer; Ralph, Stuart A.; Cowman, Alan F.; Brown, Graham V.; Duffy, Michael F.

    2011-01-01

    Plasmodium falciparum employs antigenic variation to evade the human immune response by switching the expression of different variant surface antigens encoded by the var gene family. Epigenetic mechanisms including histone modifications and sub-nuclear compartmentalization contribute to transcriptional regulation in the malaria parasite, in particular to control antigenic variation. Another mechanism of epigenetic control is the exchange of canonical histones with alternative variants to generate functionally specialized chromatin domains. Here we demonstrate that the alternative histone PfH2A.Z is associated with the epigenetic regulation of var genes. In many eukaryotic organisms the histone variant H2A.Z mediates an open chromatin structure at promoters and facilitates diverse levels of regulation, including transcriptional activation. Throughout the asexual, intraerythrocytic lifecycle of P. falciparum we found that the P. falciparum ortholog of H2A.Z (PfH2A.Z) colocalizes with histone modifications that are characteristic of transcriptionally-permissive euchromatin, but not with markers of heterochromatin. Consistent with this finding, antibodies to PfH2A.Z co-precipitate the permissive modification H3K4me3. By chromatin-immunoprecipitation we show that PfH2A.Z is enriched in nucleosomes around the transcription start site (TSS) in both transcriptionally active and silent stage-specific genes. In var genes, however, PfH2A.Z is enriched at the TSS only during active transcription in ring stage parasites. Thus, in contrast to other genes, temporal var gene regulation involves histone variant exchange at promoter nucleosomes. Sir2 histone deacetylases are important for var gene silencing and their yeast ortholog antagonises H2A.Z function in subtelomeric yeast genes. In immature P. falciparum parasites lacking Sir2A or Sir2B high var transcription levels correlate with enrichment of PfH2A.Z at the TSS. As Sir2A knock out parasites mature the var genes are

  16. Spectroscopic Classification of ASASSN-16az

    NASA Astrophysics Data System (ADS)

    Prieto, J. L.; Rich, J.; Shappee, B. J.

    2016-02-01

    We obtained an optical spectrum (range 360-920 nm) of ASASSN-16az (ATel #8614) on UT 2016 Feb 2.4 with WFCCD mounted on the du Pont 2.5m telescope at Las Campanas Observatory. Cross-correlation with a library of SN spectra using SNID (Blondin & Tonry 2007, ApJ, 666, 1024) shows that ASASSN-16az is a normal Type Ia at +10 days.

  17. Texture Evolution of Single-Pass Hot-Rolled 5052/AZ31/5052 Clad Sheets

    NASA Astrophysics Data System (ADS)

    Nie, Huihui; Liang, Wei; Yang, Fuqian; Zheng, Liuwei; Li, Xianrong; Fan, Haiwei

    2016-06-01

    Three-layered 5052/AZ31/5052 clad sheets with maximum rolling reductions of 33% and 48% were prepared, using single-pass hot rolling followed by thermal annealing at 200°C for 1 h. The evolutions of microstructures and textures were analyzed. The experimental results show that the AZ31 layer exhibited a typical deformation microstructure with rolling-induced twins. The AZ31 layer with the 33% rolling reduction possessed a texture with the basal pole tilting about 35° away from normal direction to transverse direction and the majority of twins consists of {10 bar{1} 1}-{10 bar{1} 2} double twins and {10 bar{1} 2} tensile twins. The AZ31 layer with the 48% rolling reduction possessed a typical basal texture because {10 bar{1} 1} compression twins were activated by c-axis strain to compete with the tensile twins. No intermetallics were observed after annealing, and recrystallization occurred preferentially at the interface between AZ31 and 5052. The typical rolling texture of the 5052 layer disappeared, and the stable {001} <110> rotation cube component was dominant. The tensile test of the rolled three-layered 5052/AZ31/5052 clad sheets was performed. The tensile experimental results show that the annealed clad sheets with 33% rolling reduction and smaller degree of recrystallization have the largest elongation of 22.5% and larger ultimate tensile strength (UTS) than the annealed clad sheets with 48% rolling reduction.

  18. 78 FR 51707 - Foreign-Trade Zone 59-Lincoln, Nebraska; Authorization of Production Activity; CNH America, LLC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-21

    ... FTZ Board (15 CFR part 400), including notice in the Federal Register inviting public comment (78 FR... Foreign-Trade Zones Board Foreign-Trade Zone 59--Lincoln, Nebraska; Authorization of Production Activity..., 2013, the Lincoln-Foreign Trade Zone, Inc., grantee of FTZ 59, submitted a notification of...

  19. 77 FR 75972 - Foreign-Trade Zone 26 - Atlanta, Georgia Notification of Proposed Production Activity Suzuki Mfg...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 26 -- Atlanta, Georgia Notification of Proposed Production Activity Suzuki Mfg. of America Corp. (All-Terrain Vehicles) Rome, Jonesboro and Cartersville, Georgia Georgia Foreign-Trade Zone, Inc., grantee of...

  20. 77 FR 28569 - Foreign-Trade Zone 92-Gulfport, MS Notification of Proposed Production Activity; Gulf Ship, LLC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 92--Gulfport, MS Notification of Proposed Production Activity; Gulf Ship, LLC, (Shipbuilding), Gulfport, MS The Mississippi Coast Foreign-Trade Zone, Inc., grantee...

  1. 77 FR 59890 - Foreign-Trade Zone 92-Gulfport, MS; Authorization of Production Activity; Gulf Ship, LLC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-01

    ... notice in the Federal Register inviting public comment (77 FR 28569, 5-15-2012). The FTZ Board has... Foreign-Trade Zones Board Foreign-Trade Zone 92--Gulfport, MS; Authorization of Production Activity; Gulf Ship, LLC (Shipbuilding); Gulfport, MS On May 10, 2012, the Mississippi Coast Foreign-Trade Zone,...

  2. Tsunamigenic potential of Mediterranean fault systems and active subduction zones

    NASA Astrophysics Data System (ADS)

    Petricca, Patrizio; Babeyko, Andrey

    2016-04-01

    Since the North East Atlantic and Mediterranean Tsunami Warning System (NEAMTWS) is under development by the European scientific community, it becomes necessary to define guidelines for the characterization of the numerous parameters must be taken into account in a fair assessment of the risk. Definition of possible tectonic sources and evaluation of their potential is one of the principal issues. In this study we systematically evaluate tsunamigenic potential of up-to-now known real fault systems and active subduction interfaces in the NEAMTWS region. The task is accomplished by means of numerical modeling of tsunami generation and propagation. We have simulated all possible uniform-slip ruptures populating fault and subduction interfaces with magnitudes ranging from 6.5 up to expected Mmax. A total of 15810 individual ruptures were processed. For each rupture, a tsunami propagation scenario was computed in linear shallow-water approximation on 1-arc minute bathymetric grid (Gebco_08) implying normal reflection boundary conditions. Maximum wave heights at coastal positions (totally - 23236 points of interest) were recorded for four hours of simulation and then classified according to currently adopted warning level thresholds. The resulting dataset allowed us to classify the sources in terms of their tsunamigenic potential as well as to estimate their minimum tsunamigenic magnitude. Our analysis shows that almost every source in the Mediterranean Sea is capable to produce local tsunami at the advisory level (i.e., wave height > 20 cm) starting from magnitude values of Mw=6.6. In respect to the watch level (wave height > 50 cm), the picture is less homogeneous: crustal sources in south-west Mediterranean as well as East-Hellenic arc need larger magnitudes (around Mw=7.0) to trigger watch levels even at the nearby coasts. In the context of the regional warning (i.e., source-to-coast distance > 100 km) faults also behave more heterogeneously in respect to the minimum

  3. Perfil de Franklin Chang- Díaz de Franklin Chang-Díaz

    NASA Video Gallery

    Franklin Chang-Díaz, el primer astronauta hispano de la NASA, habla sobre qué lo inspiró a trabajar con cohetes y convertirse en astronauta. Chang-Díaz alienta a los jóvenes para que sigan sus sueños.

  4. Amplitude analysis of active source seismic data from the grounding zone of Whillans Ice Stream

    NASA Astrophysics Data System (ADS)

    Horgan, Huw; Anandakrishnan, Sridhar; Alley, Richard; Christianson, Knut

    2015-04-01

    Amplitude analysis of active source seismic data is often used to estimate acoustic properties and thereby infer the lithology of the substrate beneath glaciers and ice streams. The substrate beneath the ice streams of West Antarctica is of particular interest as here subglacial sediment deformation results in the rapid flow of the overriding ice. At the grounding zone, where the grounded ice sheet transitions to the floating ice shelf, this substrate is thought to stiffen due to tidal compaction resulting in a zone of higher basal shear stress which is manifest in the buckling of the internal layering in the overriding ice. Here we investigate these processes by estimating subglacial properties using active source seismic data acquired across the grounding zone of Whillans Ice Stream. Perhaps uniquely, we are able to test our methodology due to the survey crossing from an ice overlying sediment interface into a known ice overlying water interface. Our analysis indicates that lithological variations within the grounding zone are below the resolution of our methodology with the exception of a body of water trapped by a hydropotential reversal upstream of the grounding zone.

  5. Dynamical Organization of Syntaxin-1A at the Presynaptic Active Zone

    PubMed Central

    Ullrich, Alexander; Böhme, Mathias A.; Schöneberg, Johannes; Depner, Harald; Sigrist, Stephan J.; Noé, Frank

    2015-01-01

    Synaptic vesicle fusion is mediated by SNARE proteins forming in between synaptic vesicle (v-SNARE) and plasma membrane (t-SNARE), one of which is Syntaxin-1A. Although exocytosis mainly occurs at active zones, Syntaxin-1A appears to cover the entire neuronal membrane. By using STED super-resolution light microscopy and image analysis of Drosophila neuro-muscular junctions, we show that Syntaxin-1A clusters are more abundant and have an increased size at active zones. A computational particle-based model of syntaxin cluster formation and dynamics is developed. The model is parametrized to reproduce Syntaxin cluster-size distributions found by STED analysis, and successfully reproduces existing FRAP results. The model shows that the neuronal membrane is adjusted in a way to strike a balance between having most syntaxins stored in large clusters, while still keeping a mobile fraction of syntaxins free or in small clusters that can efficiently search the membrane or be traded between clusters. This balance is subtle and can be shifted toward almost no clustering and almost complete clustering by modifying the syntaxin interaction energy on the order of only 1 kBT. This capability appears to be exploited at active zones. The larger active-zone syntaxin clusters are more stable and provide regions of high docking and fusion capability, whereas the smaller clusters outside may serve as flexible reserve pool or sites of spontaneous ectopic release. PMID:26367029

  6. Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling.

    PubMed

    Delvendahl, Igor; Jablonski, Lukasz; Baade, Carolin; Matveev, Victor; Neher, Erwin; Hallermann, Stefan

    2015-06-01

    Fast synchronous neurotransmitter release at the presynaptic active zone is triggered by local Ca(2+) signals, which are confined in their spatiotemporal extent by endogenous Ca(2+) buffers. However, it remains elusive how rapid and reliable Ca(2+) signaling can be sustained during repetitive release. Here, we established quantitative two-photon Ca(2+) imaging in cerebellar mossy fiber boutons, which fire at exceptionally high rates. We show that endogenous fixed buffers have a surprisingly low Ca(2+)-binding ratio (∼ 15) and low affinity, whereas mobile buffers have high affinity. Experimentally constrained modeling revealed that the low endogenous buffering promotes fast clearance of Ca(2+) from the active zone during repetitive firing. Measuring Ca(2+) signals at different distances from active zones with ultra-high-resolution confirmed our model predictions. Our results lead to the concept that reduced Ca(2+) buffering enables fast active zone Ca(2+) signaling, suggesting that the strength of endogenous Ca(2+) buffering limits the rate of synchronous synaptic transmission. PMID:26015575

  7. 78 FR 16247 - Foreign-Trade Zone 38-Spartanburg County, South Carolina; Authorization of Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-14

    ... Federal Register inviting public comment (77 FR 70992-70993, 11-28-2012). The FTZ Board has determined... On November 8, 2012, the South Carolina State Ports Authority, grantee of FTZ 38, submitted a notification of proposed production activity to the Foreign-Trade Zones (FTZ) Board on behalf of...

  8. Dynamical Organization of Syntaxin-1A at the Presynaptic Active Zone.

    PubMed

    Ullrich, Alexander; Böhme, Mathias A; Schöneberg, Johannes; Depner, Harald; Sigrist, Stephan J; Noé, Frank

    2015-09-01

    Synaptic vesicle fusion is mediated by SNARE proteins forming in between synaptic vesicle (v-SNARE) and plasma membrane (t-SNARE), one of which is Syntaxin-1A. Although exocytosis mainly occurs at active zones, Syntaxin-1A appears to cover the entire neuronal membrane. By using STED super-resolution light microscopy and image analysis of Drosophila neuro-muscular junctions, we show that Syntaxin-1A clusters are more abundant and have an increased size at active zones. A computational particle-based model of syntaxin cluster formation and dynamics is developed. The model is parametrized to reproduce Syntaxin cluster-size distributions found by STED analysis, and successfully reproduces existing FRAP results. The model shows that the neuronal membrane is adjusted in a way to strike a balance between having most syntaxins stored in large clusters, while still keeping a mobile fraction of syntaxins free or in small clusters that can efficiently search the membrane or be traded between clusters. This balance is subtle and can be shifted toward almost no clustering and almost complete clustering by modifying the syntaxin interaction energy on the order of only 1 kBT. This capability appears to be exploited at active zones. The larger active-zone syntaxin clusters are more stable and provide regions of high docking and fusion capability, whereas the smaller clusters outside may serve as flexible reserve pool or sites of spontaneous ectopic release. PMID:26367029

  9. Microbial respiration and extracellular enzyme activity in sediments from the Gulf of Mexico hypoxic zone

    EPA Science Inventory

    This study explores the relationship between sediment chemistry (TC, TN, TP) and microbial respiration (DHA) and extracellular enzyme activity (EEA) across the Gulf of Mexico (GOM) hypoxic zone. TC, TN, and TP were all positively correlated with each other (r=0.19-0.68). DHA was ...

  10. 77 FR 47429 - Agency Information Collection Activities; Petroleum Refineries in Foreign Trade Sub-zones

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ... concerning the Petroleum Refineries in Foreign Trade Sub-zones. This request for comment is being made... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities; Petroleum Refineries... CBP is soliciting comments concerning the following information collection: Title:...

  11. RIM Promotes Calcium Channel Accumulation at Active Zones of the Drosophila Neuromuscular Junction

    PubMed Central

    Graf, Ethan R.; Valakh, Vera; Wright, Christina M.; Wu, Chunlai; Liu, Zhihua; Zhang, Yong Q.; DiAntonio, Aaron

    2012-01-01

    Summary Synaptic communication requires the controlled release of synaptic vesicles from presynaptic axon terminals. Release efficacy is regulated by the many proteins that comprise the presynaptic release apparatus, including Ca2+ channels and proteins that influence Ca2+ channel accumulation at release sites. Here we identify Drosophila RIM and demonstrate that it localizes to active zones at the larval neuromuscular junction. In Drosophila RIM mutants, there is a large decrease in evoked synaptic transmission, due to a significant reduction in both the clustering of Ca2+ channels and the size of the readily releasable pool of synaptic vesicles at active zones. Hence, RIM plays an evolutionarily conserved role in regulating synaptic calcium channel localization and readily releasable pool size. Since RIM has traditionally been studied as an effector of Rab3 function, we investigate whether RIM is involved in the newly identified function of Rab3 in the distribution of presynaptic release machinery components across release sites. Bruchpilot (Brp), an essential component of the active zone cytomatrix T bar, is unaffected by RIM disruption, indicating that Brp localization and distribution across active zones does not require wild type RIM. In addition, larvae containing mutations in both RIM and rab3 have reduced Ca2+ channel levels and a Brp distribution that is very similar to that of the rab3 single mutant, indicating that RIM functions to regulate Ca2+ channel accumulation but is not a Rab3 effector for release machinery distribution across release sites. PMID:23175814

  12. 78 FR 49255 - Foreign-Trade Zone 158-Vicksburg/Jackson, Mississippi; Authorization of Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-13

    ... (15 CFR part 400), including notice in the Federal Register inviting public comment (78 FR 20889, 4-8... Production Activity; Extension of Production Authority; Lane Furniture Industries, Inc. (Upholstered... Foreign-Trade Zones (FTZ) Board on behalf of Lane Furniture Industries, Inc., in Belden, Saltillo,...

  13. Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling

    PubMed Central

    Delvendahl, Igor; Jablonski, Lukasz; Baade, Carolin; Matveev, Victor; Neher, Erwin; Hallermann, Stefan

    2015-01-01

    Fast synchronous neurotransmitter release at the presynaptic active zone is triggered by local Ca2+ signals, which are confined in their spatiotemporal extent by endogenous Ca2+ buffers. However, it remains elusive how rapid and reliable Ca2+ signaling can be sustained during repetitive release. Here, we established quantitative two-photon Ca2+ imaging in cerebellar mossy fiber boutons, which fire at exceptionally high rates. We show that endogenous fixed buffers have a surprisingly low Ca2+-binding ratio (∼15) and low affinity, whereas mobile buffers have high affinity. Experimentally constrained modeling revealed that the low endogenous buffering promotes fast clearance of Ca2+ from the active zone during repetitive firing. Measuring Ca2+ signals at different distances from active zones with ultra-high-resolution confirmed our model predictions. Our results lead to the concept that reduced Ca2+ buffering enables fast active zone Ca2+ signaling, suggesting that the strength of endogenous Ca2+ buffering limits the rate of synchronous synaptic transmission. PMID:26015575

  14. Radioisotope inventory of T101AZ thermocouple tree from riser 13D

    SciTech Connect

    Kessler, S.F., Westinghouse Hanford

    1996-06-12

    The radionuclide inventory for the thermocouple tree removed from tank T101-AZ riser 13D was estimated using measured {sup 137}Cs activity. This activity was measured by detectors as the tree was removed from the tank. Other radionuclide activities were estimated using the results of tank samples.

  15. Hydrogen Gas Emissions from Active Faults and Identification of Flow Pathway in a Fault Zone

    NASA Astrophysics Data System (ADS)

    Ishimaru, T.; Niwa, M.; Kurosawa, H.; Shimada, K.

    2010-12-01

    It has been observed that hydrogen gas emissions from the subsurface along active faults exceed atmospheric concentrations (e.g. Sugisaki et. al., 1983). Experimental studies have shown that hydrogen gas is generated in a radical reaction of water with fractured silicate minerals due to rock fracturing caused by fault movement (e.g. Kita et al., 1982). Based on such research, we are studying an investigation method for an assessment of fault activity using hydrogen gas emissions from fracture zones. To start, we have devised portable equipment for rapid and simple in situ measurement of hydrogen gas emissions (Shimada et al., 2008). The key component of this equipment is a commercially available and compact hydrogen gas sensor with an integral data logger operable at atmospheric pressure. In the field, we have drilled shallow boreholes into incohesive fault rocks to depths ranging from 15 to 45 cm using a hand-operated drill with a 9mm drill-bit. Then, we have measured the hydrogen gas concentrations in emissions from active faults such as: the western part of the Atotsugawa fault zone, the Atera fault zone and the Neodani fault in central Japan; the Yamasaki fault zone in southwest Japan; and the Yamagata fault zone in northeast Japan. In addition, we have investigated the hydrogen gas concentrations in emissions from other major geological features such as tectonic lines: the Butsuzo Tectonic Line in the eastern Kii Peninsula and the Atokura Nappe in the Northeastern Kanto Mountains. As a result of the investigations, hydrogen gas concentration in emissions from the active faults was measured to be in the approximate range from 6,000 ppm to 26,000 ppm in two to three hours after drilling. A tendency for high concentrations of hydrogen gas in active faults was recognized, in contrast with low concentrations in emissions from tectonic lines that were observed to be in the range from 730 ppm to 2,000 ppm. It is inferred that the hydrogen gas migrates to ground

  16. 34 CFR 299.3 - What priority may the Secretary establish for activities in an Empowerment Zone or Enterprise...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... activities in an Empowerment Zone or Enterprise Community? For any ESEA discretionary grant program, the Secretary may establish a priority, as authorized by 34 CFR 75.105(b), for projects that will— (a) Use a... activities in an Empowerment Zone or Enterprise Community? 299.3 Section 299.3 Education Regulations of...

  17. 34 CFR 299.3 - What priority may the Secretary establish for activities in an Empowerment Zone or Enterprise...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... activities in an Empowerment Zone or Enterprise Community? For any ESEA discretionary grant program, the Secretary may establish a priority, as authorized by 34 CFR 75.105(b), for projects that will— (a) Use a... activities in an Empowerment Zone or Enterprise Community? 299.3 Section 299.3 Education Regulations of...

  18. 34 CFR 299.3 - What priority may the Secretary establish for activities in an Empowerment Zone or Enterprise...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... activities in an Empowerment Zone or Enterprise Community? For any ESEA discretionary grant program, the Secretary may establish a priority, as authorized by 34 CFR 75.105(b), for projects that will— (a) Use a... activities in an Empowerment Zone or Enterprise Community? 299.3 Section 299.3 Education Regulations of...

  19. 34 CFR 299.3 - What priority may the Secretary establish for activities in an Empowerment Zone or Enterprise...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... activities in an Empowerment Zone or Enterprise Community? For any ESEA discretionary grant program, the Secretary may establish a priority, as authorized by 34 CFR 75.105(b), for projects that will— (a) Use a... activities in an Empowerment Zone or Enterprise Community? 299.3 Section 299.3 Education Regulations of...

  20. 34 CFR 299.3 - What priority may the Secretary establish for activities in an Empowerment Zone or Enterprise...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... activities in an Empowerment Zone or Enterprise Community? For any ESEA discretionary grant program, the Secretary may establish a priority, as authorized by 34 CFR 75.105(b), for projects that will— (a) Use a... activities in an Empowerment Zone or Enterprise Community? 299.3 Section 299.3 Education Regulations of...

  1. 77 FR 52680 - Foreign-Trade Zone 242-Boundary County, ID, Notification of Proposed Production Activity, AREVA...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-30

    ... Foreign-Trade Zones Board Foreign-Trade Zone 242--Boundary County, ID, Notification of Proposed Production Activity, AREVA Enrichment Services, LLC, (Gas Centrifuge Production Equipment), Bonneville County, ID Boundary County, grantee of FTZ 242, submitted a notification of proposed production activity on behalf...

  2. 77 FR 39209 - Foreign-Trade Zone 74-Baltimore, MD, Notification of Proposed Production Activity, J.D. Neuhaus...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-02

    ... Foreign-Trade Zones Board Foreign-Trade Zone 74--Baltimore, MD, Notification of Proposed Production Activity, J.D. Neuhaus LP (Overhead Lifting Equipment Production) Sparks, MD The Baltimore Development Corporation, grantee of FTZ 74, submitted a notification of proposed production activity on behalf of...

  3. 77 FR 28353 - Foreign-Trade Zone 45-Portland, OR, Notification of Proposed Production Activity, Shimadzu USA...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-14

    ... Foreign-Trade Zones Board Foreign-Trade Zone 45--Portland, OR, Notification of Proposed Production Activity, Shimadzu USA Manufacturing, Inc. (Chromatograph and Mass Spectrometer Production), Canby, OR The Port of Portland, grantee of FTZ 45, submitted a notification of proposed production activity on...

  4. 75 FR 19246 - Safety Zone; Desert Storm, Lake Havasu, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-14

    ... vessels and users of the waterway. Persons and vessels are prohibited from entering into, transiting... the safety of the crew, spectators, and other vessels and users of the waterway. Background and..., and other users of the waterway. This event involves powerboats participating in an exhibition run...

  5. Accumulation of Exogenous Activated TGF-β in the Superficial Zone of Articular Cartilage

    PubMed Central

    Albro, Michael B.; Nims, Robert J.; Cigan, Alexander D.; Yeroushalmi, Kevin J.; Alliston, Tamara; Hung, Clark T.; Ateshian, Gerard A.

    2013-01-01

    It was recently demonstrated that mechanical shearing of synovial fluid (SF), induced during joint motion, rapidly activates latent transforming growth factor β (TGF-β). This discovery raised the possibility of a physiological process consisting of latent TGF-β supply to SF, activation via shearing, and transport of TGF-β into the cartilage matrix. Therefore, the two primary objectives of this investigation were to characterize the secretion rate of latent TGF-β into SF, and the transport of active TGF-β across the articular surface and into the cartilage layer. Experiments on tissue explants demonstrate that high levels of latent TGF-β1 are secreted from both the synovium and all three articular cartilage zones (superficial, middle, and deep), suggesting that these tissues are capable of continuously replenishing latent TGF-β to SF. Furthermore, upon exposure of cartilage to active TGF-β1, the peptide accumulates in the superficial zone (SZ) due to the presence of an overwhelming concentration of nonspecific TGF-β binding sites in the extracellular matrix. Although this response leads to high levels of active TGF-β in the SZ, the active peptide is unable to penetrate deeper into the middle and deep zones of cartilage. These results provide strong evidence for a sequential physiologic mechanism through which SZ chondrocytes gain access to active TGF-β: the synovium and articular cartilage secrete latent TGF-β into the SF and, upon activation, TGF-β transports back into the cartilage layer, binding exclusively to the SZ. PMID:23601326

  6. Kinetics of recrystallization for twin-roll casting AZ31 magnesium alloy during homogenization

    NASA Astrophysics Data System (ADS)

    Zhao, Hu; Li, Pei-Jie; He, Liang-Ju

    2011-10-01

    The kinetics of recrystallization for twin-roll casting AZ31 magnesium alloy with different thicknesses during homogenization was analyzed. It is shown that fine grains are first formed at the boundaries of deformed bands in the twin-roll casting slab. The recrystallized grains with no strain are gradually substituted for the deformed microstructure of twin-roll casting AZ31 magnesium alloy. The incubation temperature and time for the recrystallization of a twin-roll casting AZ31 magnesium alloy strip with a thickness of 3 mm are lower and shorter than those of the 6-mm thick strip, respectively. The 3-mm thick twin-roll casting magnesium alloy has finer grains than the 6-mm thick strip. The activation energies of recrystallization for twin-roll casting AZ31 magnesium alloy slabs with the thickness of 3 and 6 mm are 88 and 69 kJ/mol, respectively. The kinetics curves of recrystallization for twin-roll casting AZ31 magnesium alloy were obtained.

  7. Histone H2A.Z subunit exchange controls consolidation of recent and remote memory.

    PubMed

    Zovkic, Iva B; Paulukaitis, Brynna S; Day, Jeremy J; Etikala, Deepa M; Sweatt, J David

    2014-11-27

    Memory formation is a multi-stage process that initially requires cellular consolidation in the hippocampus, after which memories are downloaded to the cortex for maintenance, in a process termed systems consolidation. Epigenetic mechanisms regulate both types of consolidation, but histone variant exchange, in which canonical histones are replaced with their variant counterparts, is an entire branch of epigenetics that has received limited attention in the brain and has never, to our knowledge, been studied in relation to cognitive function. Here we show that histone H2A.Z, a variant of histone H2A, is actively exchanged in response to fear conditioning in the hippocampus and the cortex, where it mediates gene expression and restrains the formation of recent and remote memory. Our data provide evidence for H2A.Z involvement in cognitive function and specifically implicate H2A.Z as a negative regulator of hippocampal consolidation and systems consolidation, probably through downstream effects on gene expression. Moreover, alterations in H2A.Z binding at later stages of systems consolidation suggest that this histone has the capacity to mediate stable molecular modifications required for memory retention. Overall, our data introduce histone variant exchange as a novel mechanism contributing to the molecular basis of cognitive function and implicate H2A.Z as a potential therapeutic target for memory disorders. PMID:25219850

  8. Micro 3D ERT tomography for data assimilation modelling of active root zone

    NASA Astrophysics Data System (ADS)

    Vanella, Daniela; Busato, Laura; Boaga, Jacopo; Cassiani, Giorgio; Binley, Andrew; Putti, Mario; Consoli, Simona

    2016-04-01

    Within the soil-plant-atmosphere system, root activity plays a fundamental role, as it connects different domains and allows a large part of the water and nutrient exchanges necessary for plant sustenance. The understanding of these processes is not only useful from an environmental point of view, making a fundamental contribution to the understanding of the critical zone dynamics, but also plays a pivotal role in precision agriculture, where the optimisation of water resources exploitation is mandatory and often carried out through deficit irrigation techniques. In this work, we present the results of non-invasive monitoring of the active root zone of two orange trees (Citrus sinensis, cv Tarocco Ippolito) located in an orange orchard in eastern Sicily (Italy) and drip irrigated with two different techniques: partial root drying and 100% crop evapotranspiration. The main goal of the monitoring activity is to assess possible differences between the developed root systems and the root water uptake between the two irrigation strategies. The monitoring is conducted using 3D micro-electrical resistivity tomography (ERT) based on an apparatus composed of a number of micro-boreholes (about 1.2 m deep) housing 12 electrodes each, plus a number of surface electrodes. Time-lapse measurements conducted both with long-term periodicity and short-term repetition before and after irrigation clearly highlight the presence and distribution of root water uptake zone both at shallow and larger depth, likely to correspond to zones utilized during the irrigation period (shallow) and during the time when the crop is not irrigated (deep). Subsidiary information is available in terms of precipitation, sap flow measurements and micrometeorological evapotranspiration estimates. This data ensemble lends itself to the assimilation into a variably saturated flow model, where both soil hydraulic parameters and root distribution shall be identified. Preliminary results in this directions show

  9. Micro 3D ERT tomography for data assimilation modelling of active root zone

    NASA Astrophysics Data System (ADS)

    Cassiani, G.; Boaga, J.; Busato, L.; Vanella, D.; Consoli, S.; Binley, A. M.

    2015-12-01

    Within the soil-plant-atmosphere system, root activity plays a fundamental role, as it connects different domains and allows a large part of the water and nutrient exchanges necessary for plant sustenance. The understanding of these processes is not only useful from an environmental point of view, making a fundamental contribution to the understanding of the critical zone dynamics, but also plays a pivotal role in precision agriculture, where the optimisation of water resources exploitation is mandatory and often carried out through deficit irrigation techniques. In this work, we present the results of non-invasive monitoring of the active root zone of two orange trees (Citrus sinensis, cv Tarocco Ippolito) located in an orange orchard in eastern Sicily (Italy) and drip irrigated with two different techniques: partial root drying and 100% crop evapotranspiration. The main goal of the monitoring activity is to assess possible differences between the developed root systems and the root water uptake between the two irrigation strategies. The monitoring is conducted using 3D micro-electrical resistivity tomography (ERT) based on an apparatus composed of a number of micro-boreholes (about 1.2 m deep) housing 12 electrodes each, plus a number of surface electrodes. Time-lapse measurements conducted both with long-term periodicity and short-term repetition before and after irrigation clearly highlight the presence and distribution of root water uptake zone both at shallow and larger depth, likely to correspond to zones utilized during the irrigation period (shallow) and during the time when the crop is not irrigated (deep). Subsidiary information is available in terms of precipitation, sap flow measurements and micrometeorological evapotranspiration estimates. This data ensemble lends itself to the assimilation into a variably saturated flow model, where both soil hydraulic parameters and root distribution shall be identified. Preliminary results in this directions show

  10. Biochemistry Games: "AZ-Quiz" and "Jeopardy!"

    ERIC Educational Resources Information Center

    Rostejnska, Milada; Klimova, Helena

    2011-01-01

    "AZ-Quiz" and "Jeopardy!" are popular television shows and serve as the basis for in-class games designed to support and diversify chemistry instruction at the high school level. Both games were created in Microsoft PowerPoint, which is an easily accessible and controllable instrument that enables the creation of engaging animation. The use of…

  11. 75 FR 62896 - Arizona Disaster #AZ-00012

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-13

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Arizona Disaster AZ-00012 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for...

  12. Fault zone structure and inferences on past activities of the active Shanchiao Fault in the Taipei metropolis, northern Taiwan

    NASA Astrophysics Data System (ADS)

    Chen, C.; Lee, J.; Chan, Y.; Lu, C.

    2010-12-01

    The Taipei Metropolis, home to around 10 million people, is subject to seismic hazard originated from not only distant faults or sources scattered throughout the Taiwan region, but also active fault lain directly underneath. Northern Taiwan including the Taipei region is currently affected by post-orogenic (Penglai arc-continent collision) processes related to backarc extension of the Ryukyu subduction system. The Shanchiao Fault, an active normal fault outcropping along the western boundary of the Taipei Basin and dipping to the east, is investigated here for its subsurface structure and activities. Boreholes records in the central portion of the fault were analyzed to document the stacking of post- Last Glacial Maximum growth sediments, and a tulip flower structure is illuminated with averaged vertical slip rate of about 3 mm/yr. Similar fault zone architecture and post-LGM tectonic subsidence rate is also found in the northern portion of the fault. A correlation between geomorphology and structural geology in the Shanchiao Fault zone demonstrates an array of subtle geomorphic scarps corresponds to the branch fault while the surface trace of the main fault seems to be completely erased by erosion and sedimentation. Such constraints and knowledge are crucial in earthquake hazard evaluation and mitigation in the Taipei Metropolis, and in understanding the kinematics of transtensional tectonics in northern Taiwan. Schematic 3D diagram of the fault zone in the central portion of the Shanchiao Fault, displaying regional subsurface geology and its relation to topographic features.

  13. Electrodeposition of high corrosion resistance Cu/Ni-P coating on AZ91D magnesium alloy

    NASA Astrophysics Data System (ADS)

    Zhang, Shan; Cao, Fahe; Chang, Linrong; Zheng, JunJun; Zhang, Zhao; Zhang, Jianqing; Cao, Chunan

    2011-08-01

    High corrosion resistance Cu/Ni-P coatings were electrodeposited on AZ91D magnesium alloy via suitable pretreatments, such as one-step acid pickling-activation, once zinc immersion and environment-friendly electroplated copper as the protective under-layer, which made Ni-P deposit on AZ91D Mg alloy in acid plating baths successfully. The pH value and current density for Ni-P electrodeposition were optimized to obtain high corrosion resistance. With increasing the phosphorous content of the Ni-P coatings, the deposits were found to gradually transform to amorphous structure and the corrosion resistance increased synchronously. The anticorrosion ability of AZ91D Mg alloy was greatly improved by the amorphous Ni-P deposits, which was investigated by potentiodynamic polarization curve and electrochemical impedance spectroscopy (EIS). The corrosion current density ( Icorr) of the coated Mg alloy substrate is about two orders of magnitude less than that of the uncoated.

  14. The energy balance and pressure in the solar transition zone for network and active region features

    NASA Technical Reports Server (NTRS)

    Nicolas, K. R.; Bartoe, J.-D. F.; Brueckner, G. E.; Vanhoosier, M. E.

    1979-01-01

    The electron pressure and energy balance in the solar transition zone are determined for about 125 network and active region features on the basis of high spectral and spatial resolution extreme ultraviolet spectra. Si III line intensity ratios obtained from the Naval Research Laboratory high-resolution telescope and spectrograph during a rocket flight are used as diagnostics of electron density and pressure for solar features near 3.5 x 10 to the 4th K. Observed ratios are compared with the calculated dependence of the 1301 A/1312 A and 1301 A/1296 A line intensity ratios on electron density, temperature and pressure. Electron densities ranging from 2 x 10 to the 10th/cu cm to 10 to the 12th/cu cm and active region pressures from 3 x 10 to the 15th to 10 to the 16th/cu cm K are obtained. Energy balance calculations reveal the balance of the divergence of the conductive flux and turbulent energy dissipation by radiative energy losses in a plane-parallel homogeneous transition zone (fill factor of 1), and an energy source requirement for a cylindrical zone geometry (fill factor less than 0.04).

  15. Microearthquake activity on the Orozco Fracture Zone: Preliminary results from Project ROSE

    SciTech Connect

    Not Available

    1981-05-10

    We present preliminary hypocenter determinations for 52 earthquakes recorded by a large multiinstitutional network of ocean bottom seismometers and ocean bottom hydrophones in the Orozco Fracture Zone in the eastern Pacific during late February to mid-March 1979. The network was deployed as part of the Rivera Ocean Seismic Experiment, also known as Project ROSE. The Orozco Fracture Zone is Physiographically complex, and the pattern of microearthquake hypocenters at least partly reflects this complexity. All of the well-located epicenters lie within the active transform fault segment of the fracture zone. About half of the recorded earthquakes were aligned along a narrow trough that extends eastward from the northern rise crest intersection in the approximate direction of the Cocos-Pacific relative plate motion; these events appear to be characterized by strike-slip faulting. The second major group of activity occurred in the central portion of the transform fault; the microearthquakes in this group do not display a preferred alignment parallel to the direction of spreading, and several are not obviously associated with distinct topographic features. Hypocentral depth was well resolved for many of the earthquakes reported here. Nominal depths range from 0 to 17 km below the seafloor.

  16. Influences of Welding Processes on Microstructure, Hardness, and Tensile Properties of AZ31B Magnesium Alloy

    NASA Astrophysics Data System (ADS)

    Padmanaban, G.; Balasubramanian, V.; Sarin Sundar, J. K.

    2010-03-01

    This article reports the influences of welding processes such as gas tungsten arc welding (GTAW), friction stir welding (FSW), and laser beam welding (LBW) on tensile properties of AZ31B magnesium alloy. The lowest hardness distribution profile (LHDP) is constructed across the weld section to identify the fracture path. From this investigation, it is found that LBW joints exhibited superior tensile properties compared to GTAW and FSW joints and this is mainly due to the formation of very fine grains in the fusion zone and absence of heat-affected zone (HAZ).

  17. 78 FR 58273 - Foreign-Trade Zone (FTZ) 7-Mayaguez, Puerto Rico: Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone (FTZ) 7--Mayaguez, Puerto Rico: Notification of Proposed Production Activity; Patheon Puerto Rico, Inc. (Pharmaceutical Products); Caguas and Manat , Puerto Rico The Puerto Rico Industrial Development...

  18. 78 FR 30270 - Foreign-Trade Zone (FTZ) 61-San Juan, Puerto Rico, Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone (FTZ) 61--San Juan, Puerto Rico, Notification of Proposed Production Activity, Janssen Ortho LLC (Pharmaceutical Products Production), Gurabo, Puerto Rico The Puerto Rico Trade and Export Company, grantee...

  19. 77 FR 75406 - Foreign-Trade Zone 26-Atlanta, GA; Notification of Proposed Production Activity; Perkins Shibaura...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 26--Atlanta, GA; Notification of Proposed Production Activity; Perkins Shibaura Engines LLC, (Diesel Engines), Griffin, GA Perkins Shibaura Engines LLC (Perkins Shibaura), an operator of FTZ 26, submitted...

  20. 78 FR 64197 - Foreign-Trade Zone (FTZ) 8-Toledo, Ohio, Notification of Proposed Production Activity, Whirlpool...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone (FTZ) 8--Toledo, Ohio, Notification of Proposed Production Activity, Whirlpool Corporation, Subzone 8I, (Washing Machines), Clyde and Green Springs, Ohio Whirlpool Corporation (Whirlpool) submitted...

  1. 78 FR 62583 - Foreign-Trade Zone 39-Dallas-Fort Worth, Texas; Authorization of Production Activity; Lasko...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 39--Dallas-Fort Worth, Texas; Authorization of Production Activity; Lasko Products, Inc. (Household Electric Fans); Fort Worth, Texas On May 21, 2013, Lasko Products, Inc., submitted a notification of...

  2. 77 FR 63290 - Foreign-Trade Zone 74-Baltimore, MD, Authorization of Production Activity, J.D. Neuhaus LP...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-16

    ... public comment (77 FR 39209, 7/2/2012). The FTZ Board has determined that no further review of the... Foreign-Trade Zones Board Foreign-Trade Zone 74--Baltimore, MD, Authorization of Production Activity, J.D. Neuhaus LP, (Overhead Lifting Equipment Production), Sparks, MD On June 13, 2012, the...

  3. 77 FR 63290 - Foreign-Trade Zone 121-Albany, NY; Notification of Proposed Production Activity; Albany Molecular...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 121--Albany, NY; Notification of Proposed Production Activity; Albany Molecular Research, Inc., Subzone 121A, (Pharmaceutical Chemicals Production), Rensselaer, NY Albany Molecular Research, Inc....

  4. 78 FR 7395 - Foreign-Trade Zone 129-Bellingham, WA; Notification of Proposed Production Activity; T.C. Trading...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 129--Bellingham, WA; Notification of Proposed Production Activity; T.C. Trading Company, Inc. (Eyeglass Assembly and Kitting); Blaine, WA The Port of Bellingham, grantee of FTZ 129, submitted a notification...

  5. 78 FR 58995 - Foreign-Trade Zone (FTZ) 138-Columbus, Ohio; Notification of Proposed Production Activity; Rolls...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-25

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone (FTZ) 138--Columbus, Ohio; Notification of Proposed Production Activity; Rolls Royce Energy Systems, Inc. (Industrial Gas Turbines, Power Generation Turbines, and Generator Sets); Mount Vernon, Ohio...

  6. Gas pressure and electron density at the level of the active zone of hollow cathode arc discharges

    NASA Technical Reports Server (NTRS)

    Minoo, M. H.

    1984-01-01

    A model for the longitudinal variations of the partial pressures of electrons, ions, and neutral particles is proposed as a result of an experimental study of pressure variations at the level of the active zone as a function of the various discharge parameters of a hollow cathode arc. The cathode region where the temperature passes through its maximum is called active zone. The proposed model embodies the very important variations which the partial electron and neutral particles pressures undergo at the level of the active zone.

  7. Landform development in a zone of active Gedi Fault, Eastern Kachchh rift basin, India

    NASA Astrophysics Data System (ADS)

    Kothyari, Girish Ch.; Rastogi, B. K.; Morthekai, P.; Dumka, Rakesh K.

    2016-02-01

    An earthquake of 2006 Mw 5.7 occurred along east-west trending Gedi Fault (GF) to the north of the Kachchh rift basin in western India which had the epicenter in the Wagad upland, which is approximately 60 km northeast of the 2001 Mw 7.7 earthquake site (or epicenter). Development of an active fault scarp, shifting of a river channel, offsetting of streams and uplift of the ground indicate that the terrain is undergoing active deformation. Based on detailed field investigations, three major faults that control uplifts have been identified in the GF zone. These uplifts were developed in a step-over zone of the GF, and formed due to compressive force generated by left-lateral motion within the segmented blocks. In the present research, a terrace sequence along the north flowing Karaswali river in a tectonically active GF zone has been investigated. Reconstructions based on geomorphology and terrace stratigraphy supported by optical chronology suggest that the fluvial aggradation in the Wagad area was initiated during the strengthening (at ~ 8 ka) and declining (~ 4 ka) of the Indian Summer Monsoon (ISM). The presence of younger valley fill sediments which are dated ~ 1 ka is ascribed to a short lived phase of renewed strengthening of ISM before present day aridity. Based on terrace morphology two major phases of enhanced uplift have been estimated. The older uplift event dated to 8 ka is represented by the Tertiary bedrock surfaces which accommodated the onset of valley-fill aggradation. The younger event of enhanced uplift dated to 4 ka was responsible for the incision of the older valley fill sediments and the Tertiary bedrock. These ages suggest that the average rate of uplift ranges from 0.3 to 1.1 mm/yr during the last 9 ka implying active nature of the area.

  8. Regulation of Synaptic Vesicle Docking by Different Classes of Macromolecules in Active Zone Material

    PubMed Central

    Szule, Joseph A.; Harlow, Mark L.; Jung, Jae Hoon; De-Miguel, Francisco F.; Marshall, Robert M.; McMahan, Uel J.

    2012-01-01

    The docking of synaptic vesicles at active zones on the presynaptic plasma membrane of axon terminals is essential for their fusion with the membrane and exocytosis of their neurotransmitter to mediate synaptic impulse transmission. Dense networks of macromolecules, called active zone material, (AZM) are attached to the presynaptic membrane next to docked vesicles. Electron tomography has shown that some AZM macromolecules are connected to docked vesicles, leading to the suggestion that AZM is somehow involved in the docking process. We used electron tomography on the simply arranged active zones at frog neuromuscular junctions to characterize the connections of AZM to docked synaptic vesicles and to search for the establishment of such connections during vesicle docking. We show that each docked vesicle is connected to 10–15 AZM macromolecules, which fall into four classes based on several criteria including their position relative to the presynaptic membrane. In activated axon terminals fixed during replacement of docked vesicles by previously undocked vesicles, undocked vesicles near vacated docking sites on the presynaptic membrane have connections to the same classes of AZM macromolecules that are connected to docked vesicles in resting terminals. The number of classes and the total number of macromolecules to which the undocked vesicles are connected are inversely proportional to the vesicles’ distance from the presynaptic membrane. We conclude that vesicle movement toward and maintenance at docking sites on the presynaptic membrane are directed by an orderly succession of stable interactions between the vesicles and distinct classes of AZM macromolecules positioned at different distances from the membrane. Establishing the number, arrangement and sequence of association of AZM macromolecules involved in vesicle docking provides an anatomical basis for testing and extending concepts of docking mechanisms provided by biochemistry. PMID:22438915

  9. The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.

    PubMed

    Kobayashi, Shizuka; Hida, Yamato; Ishizaki, Hiroyoshi; Inoue, Eiji; Tanaka-Okamoto, Miki; Yamasaki, Miwako; Miyazaki, Taisuke; Fukaya, Masahiro; Kitajima, Isao; Takai, Yoshimi; Watanabe, Masahiko; Ohtsuka, Toshihisa; Manabe, Toshiya

    2016-09-01

    Synaptic efficacy is determined by various factors, including the quantal size, which is dependent on the amount of neurotransmitters in synaptic vesicles at the presynaptic terminal. It is essential for stable synaptic transmission that the quantal size is kept within a constant range and that synaptic efficacy during and after repetitive synaptic activation is maintained by replenishing release sites with synaptic vesicles. However, the mechanisms for these fundamental properties have still been undetermined. We found that the active zone protein CAST (cytomatrix at the active zone structural protein) played pivotal roles in both presynaptic regulation of quantal size and recycling of endocytosed synaptic vesicles. In the CA1 region of hippocampal slices of the CAST knockout mice, miniature excitatory synaptic responses were increased in size, and synaptic depression after prolonged synaptic activation was larger, which was attributable to selective impairment of synaptic vesicle trafficking via the endosome in the presynaptic terminal likely mediated by Rab6. Therefore, CAST serves as a key molecule that regulates dynamics and neurotransmitter contents of synaptic vesicles in the excitatory presynaptic terminal in the central nervous system. PMID:27422015

  10. Validation of Airborne Visible-Infrared Imaging Spectrometer Data at Ray Mine, AZ

    NASA Technical Reports Server (NTRS)

    Lang, H.; Baloga, S.

    1999-01-01

    We validate 1997 Airborne Visible-Infrared Imaging Spectrometer (AVIRIS) reflectance spectra covering 0.4 meu - 2.4 meu from a stable, flat mineralogically characterized man-made target at Ray Mine, AZ, the site for an EPA/NASA assessment of the utility of remote sensing for monitoring acid drainage from an active open pit mine.

  11. Active Crustal Faults in the Forearc Region, Guerrero Sector of the Mexican Subduction Zone

    NASA Astrophysics Data System (ADS)

    Gaidzik, Krzysztof; Ramírez-Herrera, Maria Teresa; Kostoglodov, Vladimir

    2016-01-01

    This work explores the characteristics and the seismogenic potential of crustal faults on the overriding plate in an area of high seismic hazard associated with the occurrence of subduction earthquakes and shallow earthquakes of the overriding plate. We present the results of geomorphic, structural, and fault kinematic analyses conducted on the convergent margin between the Cocos plate and the forearc region of the overriding North American plate, within the Guerrero sector of the Mexican subduction zone. We aim to determine the active tectonic processes in the forearc region of the subduction zone, using the river network pattern, topography, and structural data. We suggest that in the studied forearc region, both strike-slip and normal crustal faults sub-parallel to the subduction zone show evidence of activity. The left-lateral offsets of the main stream courses of the largest river basins, GPS measurements, and obliquity of plate convergence along the Cocos subduction zone in the Guerrero sector suggest the activity of sub-latitudinal left-lateral strike-slip faults. Notably, the regional left-lateral strike-slip fault that offsets the Papagayo River near the town of La Venta named "La Venta Fault" shows evidence of recent activity, corroborated also by GPS measurements (4-5 mm/year of sinistral motion). Assuming that during a probable earthquake the whole mapped length of this fault would rupture, it would produce an event of maximum moment magnitude Mw = 7.7. Even though only a few focal mechanism solutions indicate a stress regime relevant for reactivation of these strike-slip structures, we hypothesize that these faults are active and suggest two probable explanations: (1) these faults are characterized by long recurrence period, i.e., beyond the instrumental record, or (2) they experience slow slip events and/or associated fault creep. The analysis of focal mechanism solutions of small magnitude earthquakes in the upper plate, for the period between 1995

  12. Along strike variation of tremor activities and thermal structures in various subduction zones

    NASA Astrophysics Data System (ADS)

    Yabe, S.; Ide, S.; Yoshioka, S.

    2012-12-01

    A family of slow earthquakes, e.g., deep low frequency tremors, low frequency earthquakes (LFEs), very low frequency earthquakes (VLFs) and slow slip events (SSEs), are observed in various subduction zones. These phenomena represent shear slip on the plate interface, and they are thought to be related to brittle-ductile transition behavior on the plate interface because they are often located near the transition zones of interplate coupling estimated from GPS data. Such slip behavior along the plate interface would be controlled by temperature. Furthermore, tremors are considered to be related to fluid dehydrated from the subducting slab, through temperature dependent chemical reactions. Therefore, tremors occurrences are expected to be influenced by temperature, though some studies have questioned about the relationship between tremor activity and temperature. Here we investigate the source locations of deep tremor using an envelope correlation method and compare them with the temperature and shear strength profiles along the plate interface calculated using a numerical model (Yoshioka and Sanshadokoro, 2002). The study areas include New Zealand, southern Chile, and Mexico, where tremor behavior changes significantly along the strike of the plate interface. Investigating such along-strike variation in individual subduction zone may clarify the temperature dependence of tremor because environmental conditions affecting tremor occurrence are similar, unlike the comparison between different subduction zones. In the Hikurangi subduction zone beneath the North Island, New Zealand, the depth of SSE are quite different along the strike, e.g., deeper in the central region and shallower in the northern region (e.g. Wallace and Beavan, 2010). We reanalyze tremors detected by previous studies (Kim et al., 2011; Ide, 2012) to estimate their absolute depth and confirm that tremors in North Island are on the plate interface in both the central and the northern regions. Thermal

  13. Mutational Analysis of Rab3 Function for Controlling Active Zone Protein Composition at the Drosophila Neuromuscular Junction

    PubMed Central

    Roche, John P.; Alsharif, Peter; Graf, Ethan R.

    2015-01-01

    At synapses, the release of neurotransmitter is regulated by molecular machinery that aggregates at specialized presynaptic release sites termed active zones. The complement of active zone proteins at each site is a determinant of release efficacy and can be remodeled to alter synapse function. The small GTPase Rab3 was previously identified as playing a novel role that controls the distribution of active zone proteins to individual release sites at the Drosophila neuromuscular junction. Rab3 has been extensively studied for its role in the synaptic vesicle cycle; however, the mechanism by which Rab3 controls active zone development remains unknown. To explore this mechanism, we conducted a mutational analysis to determine the molecular and structural requirements of Rab3 function at Drosophila synapses. We find that GTP-binding is required for Rab3 to traffick to synapses and distribute active zone components across release sites. Conversely, the hydrolytic activity of Rab3 is unnecessary for this function. Through a structure-function analysis we identify specific residues within the effector-binding switch regions that are required for Rab3 function and determine that membrane attachment is essential. Our findings suggest that Rab3 controls the distribution of active zone components via a vesicle docking mechanism that is consistent with standard Rab protein function. PMID:26317909

  14. Oscillatory dynamics of the biologically active zone in in situ bioremediation

    NASA Astrophysics Data System (ADS)

    Murray, Regan E.; Luce, Benjamin P.

    2002-10-01

    In situ bioremediation is a promising biotechnology for removing aqueous phase contaminants from groundwater. The system of three partial differential equations used to model bioremediation has a traveling wave solution which loses stability in a Hopf bifurcation, giving rise to oscillating fronts. To understand the origin of these oscillations, we construct a simplified model of the biologically active zone, a time delay differential equation with state-dependent delay. Despite its simplicity the new model mimics the dynamical characteristics of the bioremediation equations remarkably well and yields an approximate parametric expression for the oscillation onset point.

  15. The origin of spontaneous electrical activity at the end-plate zone.

    PubMed

    Brown, W F; Varkey, G P

    1981-12-01

    Two types of spontaneous electrical activity are present at the end-plate zone: low-voltage negative potentials that correspond to miniature end-plate potentials, and larger voltage negative-positive potentials. The electrogenic origin of the latter has been uncertain. The origin of these larger potentials was investigated in the rat phrenic nerve diaphragm preparation and in human gastrocnemius muscle just prior to intubation during administration of preoperative anesthesia. In the hemidiaphragm the larger voltage negative-positive potentials were rarely triggered by intracellular or tungsten microelectrodes. The negative-positive potentials, however, were clearly triggered by contact of the concentric needle electrode with muscle hemidiaphragm at the end-plate region. The potentials were abolished by curare. Likewise, the equivalent potentials observed at the human gastrocnemius end-plate zone were blocked by neuromuscular blocking agents. Therefore, these positive-negative discharges represent postsynaptic muscle fiber action potentials and not nerve fiber activity. They were probably presynaptically activated by mechanical irritation of the motor axon terminal and preterminal branches. PMID:6275771

  16. Analysis of protein phosphorylation in nerve terminal reveals extensive changes in active zone proteins upon exocytosis.

    PubMed

    Kohansal-Nodehi, Mahdokht; Chua, John Je; Urlaub, Henning; Jahn, Reinhard; Czernik, Dominika

    2016-01-01

    Neurotransmitter release is mediated by the fast, calcium-triggered fusion of synaptic vesicles with the presynaptic plasma membrane, followed by endocytosis and recycling of the membrane of synaptic vesicles. While many of the proteins governing these processes are known, their regulation is only beginning to be understood. Here we have applied quantitative phosphoproteomics to identify changes in phosphorylation status of presynaptic proteins in resting and stimulated nerve terminals isolated from the brains of Wistar rats. Using rigorous quantification, we identified 252 phosphosites that are either up- or downregulated upon triggering calcium-dependent exocytosis. Particularly pronounced were regulated changes of phosphosites within protein constituents of the presynaptic active zone, including bassoon, piccolo, and RIM1. Additionally, we have mapped kinases and phosphatases that are activated upon stimulation. Overall, our study provides a snapshot of phosphorylation changes associated with presynaptic activity and provides a foundation for further functional analysis of key phosphosites involved in presynaptic plasticity. PMID:27115346

  17. The coding genome of splenic marginal zone lymphoma: activation of NOTCH2 and other pathways regulating marginal zone development

    PubMed Central

    Rossi, Davide; Trifonov, Vladimir; Fangazio, Marco; Bruscaggin, Alessio; Rasi, Silvia; Spina, Valeria; Monti, Sara; Vaisitti, Tiziana; Arruga, Francesca; Famà, Rosella; Ciardullo, Carmela; Greco, Mariangela; Cresta, Stefania; Piranda, Daniela; Holmes, Antony; Fabbri, Giulia; Messina, Monica; Rinaldi, Andrea; Wang, Jiguang; Agostinelli, Claudio; Piccaluga, Pier Paolo; Lucioni, Marco; Tabbò, Fabrizio; Serra, Roberto; Franceschetti, Silvia; Deambrogi, Clara; Daniele, Giulia; Gattei, Valter; Marasca, Roberto; Facchetti, Fabio; Arcaini, Luca; Inghirami, Giorgio; Bertoni, Francesco; Pileri, Stefano A.; Deaglio, Silvia; Foà, Robin; Pasqualucci, Laura; Rabadan, Raul

    2012-01-01

    Splenic marginal zone lymphoma (SMZL) is a B cell malignancy of unknown pathogenesis, and thus an orphan of targeted therapies. By integrating whole-exome sequencing and copy-number analysis, we show that the SMZL exome carries at least 30 nonsilent gene alterations. Mutations in NOTCH2, a gene required for marginal-zone (MZ) B cell development, represent the most frequent lesion in SMZL, accounting for ∼20% of cases. All NOTCH2 mutations are predicted to cause impaired degradation of the NOTCH2 protein by eliminating the C-terminal PEST domain, which is required for proteasomal recruitment. Among indolent B cell lymphoproliferative disorders, NOTCH2 mutations are restricted to SMZL, thus representing a potential diagnostic marker for this lymphoma type. In addition to NOTCH2, other modulators or members of the NOTCH pathway are recurrently targeted by genetic lesions in SMZL; these include NOTCH1, SPEN, and DTX1. We also noted mutations in other signaling pathways normally involved in MZ B cell development, suggesting that deregulation of MZ B cell development pathways plays a role in the pathogenesis of ∼60% SMZL. These findings have direct implications for the treatment of SMZL patients, given the availability of drugs that can target NOTCH, NF-κB, and other pathways deregulated in this disease. PMID:22891273

  18. Mapping Active Fault Zones in Southern California Using Master Multispectral Imagery Data

    NASA Astrophysics Data System (ADS)

    Harvey, J. C.; Peltzer, G. F.; Hook, S. J.; Alley, R.; Myers, J.; Coffland, B.; Dominguez, R.; Fitzgerald, M.

    2004-12-01

    Recent studies of active fault zones using the GPS and InSAR techniques have revealed slip rates that often differ from the slip rates determined from geological observations. This discrepancy is principally due to the different time windows over which surface movements are integrated in both approaches. If surface velocities near faults vary over cycles of several hundreds of years, it becomes important to document the slip history along faults over various time scales as it has been recorded in the Quaternary deposits along the fault. To this endeavor, we have acquired sets of images of the major active faults in Southern California using the MODIS/ASTER airborne simulator (MASTER) instrument. The lines are flown at low altitude above the ground to provide 4 to 5 m spatial resolution in the 50 spectral bands (0.5 to 13 microns) of the instrument. A preliminary set of data was acquired in the summer 2003 over the Garlock and the Blackwater faults in the Mojave. A more extensive campaign carried out in September 2004 covered more than 1000 km of fault lines from the central section of the San Andreas fault to the Salton Sea area. The data are being processed to extract reflectance and emissivity information. Preliminary analysis of the 2003 data confirmed the strong potential of the MASTER thermal bands to identify changes in surface emissivity due to subtle variations of the mineral composition of the deposits. Additional information on the near surface structure of the fault zones can be obtained by combining day and night surface temperature maps, as buried sections of faults are revealed by thermal capacity contrasts between the two sides of a given fault. The paper will present the data set acquired during the 2003 and 2004 campaigns and the status of the raw data processing into geo-referenced emissivity and reflectivity maps of the fault zones.

  19. Active Focal Zone Sharpening for High-Precision Treatment Using Histotripsy

    PubMed Central

    Wang, Tzu-Yin; Xu, Zhen; Hall, Timothy L.; Fowlkes, J. Brian; Roberts, William W.; Cain, Charles A.

    2011-01-01

    The goal of this study is to develop a focal zone sharpening strategy that produces more precise lesions for pulsed cavitational ultrasound therapy, or histotripsy. Precise and well-confined lesions were produced by locally suppressing cavitation in the periphery of the treatment focus without affecting cavitation in the center. The local suppression of cavitation was achieved using cavitation nuclei preconditioning pulses to actively control cavitation in the periphery of the focus. A 1-MHz 513-element therapeutic array was used to generate both the therapy and the nuclei preconditioning pulses. For therapy, 10-cycle bursts at 100-Hz pulse repetition frequency with P−/P+ pressure of 21/76 MPa were delivered to the geometric focus of the therapeutic array. For nuclei preconditioning, a different pulse was delivered to an annular region immediately surrounding the focus before each therapy pulse. A parametric study on the effective pressure, pulse duration, and delivery time of the preconditioning pulse was conducted in red blood cell-gel phantoms, where cavitational damage was indicated by the color change resulting from local cell lysis. Results showed that a short-duration (20 µs) preconditioning pulse at a medium pressure (P−/P+ pressure of 7.2/13.6 MPa) delivered shortly before (30 µs) the therapy pulse substantially suppressed the peripheral damage by 77 ± 13% while complete fractionation in the focal center was maintained. High-speed imaging of the bubble cloud showed a substantial decrease in the maximum width of the bubble cloud by 48 ± 24% using focal zone sharpening. Experiments in ex vivo livers confirmed that highly confined lesions were produced in real tissues as well as in the phantoms. This study demonstrated the feasibility of active focal zone sharpening using cavitation nuclei preconditioning, allowing for increased treatment precision compared with the natural focal width of the therapy transducer. PMID:21342816

  20. An Integrated Geospatial System for earthquake precursors assessment in Vrancea tectonic active zone in Romania

    NASA Astrophysics Data System (ADS)

    Zoran, Maria A.; Savastru, Roxana S.; Savastru, Dan M.

    2015-10-01

    With the development of space-based technologies to measure surface geophysical parameters and deformation at the boundaries of tectonic plates and large faults, earthquake science has entered a new era. Using time series satellite data for earthquake prediction, it is possible to pursue the behaviors of earthquake precursors in the future and to announce early warnings when the differences between the predicted value and the observed value exceed the pre-define threshold value. Starting with almost one week prior to a moderate or strong earthquake a transient thermal infrared rise in LST of several Celsius degrees (oC) and the increased OLR values higher than the normal have been recorded around epicentral areas, function of the magnitude and focal depth, which disappeared after the main shock. Also are recorded associated geomagnetic and ionospheric distrurbances. Vrancea tectonic active zone in Romania is characterized by a high seismic hazard in European- Mediterranean region, being responsible of strong or moderate intermediate depth and normal earthquakes generation on a confined epicentral area. Based on recorded geophysical parameters anomalies was developed an integrated geospatial system for earthquake precursors assessment in Vrancea active seismic zone. This system integrates derived from time series MODIS Terra/Aqua, NOAA-AVHRR, ASTER, Landsat TM/ETM satellite data multi geophysical parameters (land surface temperature -LST, outgoing long-wave radiation- OLR, and mean air temperature- AT as well as geomagnetic and ionospheric data in synergy with in-situ data for surveillance and forecasting of seismic events.

  1. 78 FR 60826 - Foreign-Trade Zone 155-Calhoun/Victoria Counties, Texas; Authorization of Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-02

    ... (78 FR 35604, 06/13/2013). The FTZ Board has determined that no further review of the activity is... Production Activity; Caterpillar, Inc. (Excavator and Frame Assembly Production); Victoria, Texas On May 29... proposed production activity to the Foreign-Trade Zones (FTZ) Board on behalf of Caterpillar, Inc.,...

  2. Corrosion product layers on magnesium alloys AZ31 and AZ61: Surface chemistry and protective ability

    NASA Astrophysics Data System (ADS)

    Feliu, S.; Llorente, I.

    2015-08-01

    This paper studies the chemical composition of the corrosion product layers formed on magnesium alloys AZ31 and AZ61 following immersion in 0.6 M NaCl, with a view to better understanding their protective action. Relative differences in the chemical nature of the layers were quantified by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy dispersive analysis of X-ray (EDX) and low-angle X-ray diffraction (XRD). Corrosion behavior was investigated by Electrochemical Impedance Spectroscopy (EIS) and hydrogen evolution measurement. An inhibitive effect from the corrosion product layers was observed from EIS, principally in the case of AZ31, as confirmed by hydrogen evolution tests. A link was found between carbonate enrichment observed by XPS in the surface of the corrosion product layer, concomitant with the increase in the protective properties observed by EIS.

  3. Active/passive microwave sensor comparison of MIZ-ice concentration estimates. [Marginal Ice Zone (MIZ)

    NASA Technical Reports Server (NTRS)

    Burns, B. A.; Cavalieri, D. J.; Keller, M. R.

    1986-01-01

    Active and passive microwave data collected during the 1984 summer Marginal Ice Zone Experiment in the Fram Strait (MIZEX 84) are used to compare ice concentration estimates derived from synthetic aperture radar (SAR) data to those obtained from passive microwave imagery at several frequencies. The comparison is carried out to evaluate SAR performance against the more established passive microwave technique, and to investigate discrepancies in terms of how ice surface conditions, imaging geometry, and choice of algorithm parameters affect each sensor. Active and passive estimates of ice concentration agree on average to within 12%. Estimates from the multichannel passive microwave data show best agreement with the SAR estimates because the multichannel algorithm effectively accounts for the range in ice floe brightness temperatures observed in the MIZ.

  4. How Large Scale Flows in the Solar Convection Zone may Influence Solar Activity

    NASA Technical Reports Server (NTRS)

    Hathaway, D. H.

    2004-01-01

    Large scale flows within the solar convection zone are the primary drivers of the Sun s magnetic activity cycle. Differential rotation can amplify the magnetic field and convert poloidal fields into toroidal fields. Poleward meridional flow near the surface can carry magnetic flux that reverses the magnetic poles and can convert toroidal fields into poloidal fields. The deeper, equatorward meridional flow can carry magnetic flux toward the equator where it can reconnect with oppositely directed fields in the other hemisphere. These axisymmetric flows are themselves driven by large scale convective motions. The effects of the Sun s rotation on convection produce velocity correlations that can maintain the differential rotation and meridional circulation. These convective motions can influence solar activity themselves by shaping the large-scale magnetic field pattern. While considerable theoretical advances have been made toward understanding these large scale flows, outstanding problems in matching theory to observations still remain.

  5. Fault mirrors in seismically active fault zones: A fossil of small earthquakes at shallow depths

    NASA Astrophysics Data System (ADS)

    Kuo, Li-Wei; Song, Sheng-Rong; Suppe, John; Yeh, En-Chao

    2016-03-01

    Fault mirrors (FMs) are naturally polished and glossy fault slip surfaces that can record seismic deformation at shallow depths. They are important for investigating the processes controlling dynamic fault slip. We characterize FMs in borehole samples from the hanging wall damage zone of the active Hsiaotungshi reverse fault, Taiwan. Here we report the first documented occurrence of the combination of silica gel and melt patches coating FMs, with the silica gel resembling those observed on experimentally formed FMs that were cataclastically generated. In addition, the melt patches, which are unambiguous indicators of coseismic slip, suggest that the natural FMs were produced at seismic rates, presumably resulting from flash heating at asperities on the slip surfaces. Since flash heating is efficient at small slip, we propose that these natural FMs represent fossils of small earthquakes, formed in either coseismic faulting and folding or aftershock deformation in the active Taiwan fold-and-thrust belt.

  6. H2A.Z Acidic Patch Couples Chromatin Dynamics to Regulation of Gene Expression Programs during ESC Differentiation

    PubMed Central

    Subramanian, Vidya; Mazumder, Aprotim; Surface, Lauren E.; Butty, Vincent L.; Fields, Paul A.; Alwan, Allison; Torrey, Lillian; Thai, Kevin K.; Levine, Stuart S.; Bathe, Mark; Boyer, Laurie A.

    2013-01-01

    The histone H2A variant H2A.Z is essential for embryonic development and for proper control of developmental gene expression programs in embryonic stem cells (ESCs). Divergent regions of amino acid sequence of H2A.Z likely determine its functional specialization compared to core histone H2A. For example, H2A.Z contains three divergent residues in the essential C-terminal acidic patch that reside on the surface of the histone octamer as an uninterrupted acidic patch domain; however, we know little about how these residues contribute to chromatin structure and function. Here, we show that the divergent amino acids Gly92, Asp97, and Ser98 in the H2A.Z C-terminal acidic patch (H2A.ZAP3) are critical for lineage commitment during ESC differentiation. H2A.Z is enriched at most H3K4me3 promoters in ESCs including poised, bivalent promoters that harbor both activating and repressive marks, H3K4me3 and H3K27me3 respectively. We found that while H2A.ZAP3 interacted with its deposition complex and displayed a highly similar distribution pattern compared to wild-type H2A.Z, its enrichment levels were reduced at target promoters. Further analysis revealed that H2A.ZAP3 was less tightly associated with chromatin, suggesting that the mutant is more dynamic. Notably, bivalent genes in H2A.ZAP3 ESCs displayed significant changes in expression compared to active genes. Moreover, bivalent genes in H2A.ZAP3 ESCs gained H3.3, a variant associated with higher nucleosome turnover, compared to wild-type H2A.Z. We next performed single cell imaging to measure H2A.Z dynamics. We found that H2A.ZAP3 displayed higher mobility in chromatin compared to wild-type H2A.Z by fluorescent recovery after photobleaching (FRAP). Moreover, ESCs treated with the transcriptional inhibitor flavopiridol resulted in a decrease in the H2A.ZAP3 mobile fraction and an increase in its occupancy at target genes indicating that the mutant can be properly incorporated into chromatin. Collectively, our work suggests

  7. Active faults in the deformation zone off Noto Peninsula, Japan, revealed by high- resolution seismic profiles

    NASA Astrophysics Data System (ADS)

    Inoue, T.; Okamura, Y.; Murakami, F.; Kimura, H.; Ikehara, K.

    2008-12-01

    Recently, a lot of earthquakes occur in Japan. The deformation zone which many faults and folds have concentrated exists on the Japan Sea side of Japan. The 2007 Noto Hanto Earthquake (MJMA 6.9) and 2007 Chuetsu-oki Earthquake (MJMA 6.8) were caused by activity of parts of faults in this deformation zone. The Noto Hanto Earthquake occurred on 25 March, 2007 under the northwestern coast of Noto Peninsula, Ishikawa Prefecture, Japan. This earthquake is located in Quaternary deformation zone that is continued from northern margin of Noto Peninsula to southeast direction (Okamura, 2007a). National Institute of Advanced Industrial Science and Technology (AIST) carried out high-resolution seismic survey using Boomer and 12 channels short streamer cable in the northern part off Noto Peninsula, in order to clarify distribution and activities of active faults in the deformation zone. A twelve channels short streamer cable with 2.5 meter channel spacing developed by AIST and private corporation is designed to get high resolution seismic profiles in shallow sea area. The multi-channel system is possible to equip on a small fishing boat, because the data acquisition system is based on PC and the length of the cable is short and easy to handle. Moreover, because the channel spacing is short, this cable is very effective for a high- resolution seismic profiling survey in the shallow sea, and seismic data obtained by multi-channel cable can be improved by velocity analysis and CDP stack. In the northern part off Noto Peninsula, seismic profiles depicting geologic structure up to 100 meters deep under sea floor were obtained. The most remarkable reflection surface recognized in the seismic profiles is erosion surface at the Last Glacial Maximum (LGM). In the western part, sediments about 30 meters (40 msec) thick cover the erosional surface that is distributed under the shelf shallower than 100m in depth and the sediments thin toward offshore and east. Flexures like deformation in

  8. Variability in Shallow Subduction Zone Locking Inferred From Earthquake Activity Near Nicoya Peninsula, Costa Rica

    NASA Astrophysics Data System (ADS)

    Ghosh, A.; Newman, A. V.; Thomas, A. M.; Farmer, G. T.

    2006-12-01

    At the collisional plate interface of subduction zones, the majority of the world's large and great earthquakes are produced. Thus, to understand the processes that control earthquake generation here, it is important to improve our characterization of activity along the interface. We evaluate ~1000 earthquakes recorded in the shallow subduction environment of the Middle America Trench (MAT) near Nicoya Peninsula, Costa Rica, in terms of its frequency-magnitude distribution (Log10N=a-bM) of the microseismicity. Globally, earthquake distributions have b-values near 1, meaning a 10-fold decrease in activity with each unit magnitude, M increase, and can be used to characterize the strength of locking. The unique geometry of Nicoya over the active seismogenic interface gives us a rare opportunity to explore the region with unparalleled precision. From more than 7000 earthquakes recorded by the 1999-2001 CRSEIZE project, we estimated magnitudes, and precisely relocated events using a locally derived 3D V_p and V_p/V_s velocity model (DeShon et al., 2006). Using geometric constraints and events with lowest horizontal error (<2 km σ), we created a subset of best resolved slab and interface activity. Using a methodology similar to Wiemer et al. (2001), we determined the mean and spatial variability of b. We find that generally the interface below Nicoya has b=1.4, much higher than subduction zone averages of b=0.5 to 0.8 ( Bayrak et al., 2002), thus inferring a generally weak interface. More interestingly, there was strong spatial variability in b (and hence coupling). A well resolved zone of lower b (~1), is observed offshore the central Nicoya coast, in a region previously identified as strongly coupled by modeling of GPS observed deformation (Norabuena et al., 2004). Extremely high values are on either side (b > 2), near previous large interface earthquakes in 1990 and 1992. We infer that the low b-value area offshore central Nicoya identifies a more strongly

  9. Late Quaternary tectonic activity and paleoseismicity of the Eastern Messinia Fault Zone, SW Peloponessus (Messinia, Greece).

    NASA Astrophysics Data System (ADS)

    Valkaniotis, Sotirios; Betzelou, Konstantina; Zygouri, Vassiliki; Koukouvelas, Ioannis; Ganas, Athanassios

    2015-04-01

    The southwestern part of Peloponnesus, Messinia and Laconia, is an area of significant tectonic activity situated near the Hellenic trench. Most of the deformation in this area is accommodated by the Eastern Messinia Fault Zone, bordering the western part of Taygetos Mt range and the west coast of Mani peninsula. The Eastern Messinia Fault Zone (EMFZ) is a complex system of primarily normal faults dipping westwards with a strike of NNW-SSE to N-S direction attaining a total length of more than 100 km from the northern Messinia plain in the north to the southern part of Mani peninsula in the south. The continuity of the EMFZ is disrupted by overlapping faults and relay ramp structures. The central part of the EMFZ, from the town of Oichalia to the city of Kalamata, was investigated by detailed field mapping of fault structures and post-alpine sediment formations together with re-evaluation of historical and modern seismicity. Several fault segments with lengths of 6 to 10 km were mapped, defined and evaluated according to their state of activity and age. Analysis of fault striation measurements along fault planes of the fault zone shows a present regime of WSW-ENE extension, in accordance with focal mechanisms from modern seismicity. Known faults like the Katsareika and Verga faults near the city of Kalamata are interpreted as older-generation faults that are re-activated (e.g. the 1986 Ms 6.0 Kalamata earthquake on Verga Fault) as part of a system of distributed deformation. New fault segments, some of them previously unmapped like the Asprohoma fault to the west of Kalamata, and offshore faults like Kitries and Kourtissa, are being assigned to the EMFZ. Moreover, a paleoseismological trench was excavated in the northern part of Pidima fault segment, one of the most prominent active segments of the central part of the EMFZ, in order to examine the paleoearthquake record of the fault system. A significant number of historical and instrumental earthquakes in the area

  10. Influence of Microstructure of Friction Stir Welded Joints on Growth and Properties of Microarc Oxidation Coatings on AZ31B Magnesium Alloy

    NASA Astrophysics Data System (ADS)

    Chen, Tingfang; Li, Yongliang; Xue, Wenbin; Yang, Chaolin; Qu, Yao; Hua, Ming

    2015-03-01

    Ceramic coatings on friction stir welded (FSW) joints of AZ31B magnesium alloy were fabricated by microarc oxidation (MAO) method in silicate electrolyte. Microstructure, phase constituents, microhardness and electrochemical corrosion behaviors of bare and coated magnesium alloys at different zones of FSW joints for different oxidation time were investigated. The influence of microstructure at different zones on the growth of MAO coatings was analyzed. The results show that the MAO coatings on FSW joints are uniform, and they have almost the same morphology, phase constituents, hardness and corrosion resistance at base metal, stir zone and heat-affected zone. The properties of MAO coatings are independent on the microstructures of AZ31B alloy. In addition, the microstructures of magnesium alloy near the coating/alloy interface at different zones of FSW joint was not changed by microarc discharge process.

  11. Stellar Activity Mimics a Habitable-zone Planet around Kapteyn's Star

    NASA Astrophysics Data System (ADS)

    Robertson, Paul; Roy, Arpita; Mahadevan, Suvrath

    2015-06-01

    Kapteyn’s star is an old M subdwarf believed to be a member of the Galactic halo population of stars. A recent study has claimed the existence of two super-Earth planets around the star based on radial velocity (RV) observations. The innermost of these candidate planets—Kapteyn b (P = 48 days)—resides within the circumstellar habitable zone (HZ). Given recent progress in understanding the impact of stellar activity in detecting planetary signals, we have analyzed the observed HARPS data for signatures of stellar activity. We find that while Kapteyn’s star is photometrically very stable, a suite of spectral activity indices reveal a large-amplitude rotation signal, and we determine the stellar rotation period to be 143 days. The spectral activity tracers are strongly correlated with the purported RV signal of “planet b,” and the 48-day period is an integer fraction (1/3) of the stellar rotation period. We conclude that Kapteyn b is not a planet in the HZ, but an artifact of stellar activity.

  12. Alignment of Synaptic Vesicle Macromolecules with the Macromolecules in Active Zone Material that Direct Vesicle Docking

    PubMed Central

    Xu, Jing; Jung, Jae Hoon; Marshall, Robert M.; McMahan, Uel J.

    2013-01-01

    Synaptic vesicles dock at active zones on the presynaptic plasma membrane of a neuron’s axon terminals as a precondition for fusing with the membrane and releasing their neurotransmitter to mediate synaptic impulse transmission. Typically, docked vesicles are next to aggregates of plasma membrane-bound macromolecules called active zone material (AZM). Electron tomography on tissue sections from fixed and stained axon terminals of active and resting frog neuromuscular junctions has led to the conclusion that undocked vesicles are directed to and held at the docking sites by the successive formation of stable connections between vesicle membrane proteins and proteins in different classes of AZM macromolecules. Using the same nanometer scale 3D imaging technology on appropriately stained frog neuromuscular junctions, we found that ∼10% of a vesicle’s luminal volume is occupied by a radial assembly of elongate macromolecules attached by narrow projections, nubs, to the vesicle membrane at ∼25 sites. The assembly’s chiral, bilateral shape is nearly the same vesicle to vesicle, and nubs, at their sites of connection to the vesicle membrane, are linked to macromolecules that span the membrane. For docked vesicles, the orientation of the assembly’s shape relative to the AZM and the presynaptic membrane is the same vesicle to vesicle, whereas for undocked vesicles it is not. The connection sites of most nubs on the membrane of docked vesicles are paired with the connection sites of the different classes of AZM macromolecules that regulate docking, and the membrane spanning macromolecules linked to these nubs are also attached to the AZM macromolecules. We conclude that the luminal assembly of macromolecules anchors in a particular arrangement vesicle membrane macromolecules, which contain the proteins that connect the vesicles to AZM macromolecules during docking. Undocked vesicles must move in a way that aligns this arrangement with the AZM macromolecules for

  13. Transcriptome Analysis of Tomato Flower Pedicel Tissues Reveals Abscission Zone-Specific Modulation of Key Meristem Activity Genes

    PubMed Central

    Sun, Xiuli; Zhang, Rongzhi; Wu, Liang; Liang, Yanchun; Mao, Long

    2013-01-01

    Tomato flower abscises at the anatomically distinct abscission zone that separates the pedicel into basal and apical portions. During abscission, cell separation occurs only at the abscission zone indicating distinctive molecular regulation in its cells. We conducted a transcriptome analysis of tomato pedicel tissues during ethylene promoted abscission. We found that the abscission zone was the most active site with the largest set of differentially expressed genes when compared with basal and apical portions. Gene Ontology analyses revealed enriched transcription regulation and hydrolase activities in the abscission zone. We also demonstrate coordinated responses of hormone and cell wall related genes. Besides, a number of ESTs representing homologs of key Arabidopsis shoot apical meristem activity genes were found to be preferentially expressed in the abscission zone, including WUSCHEL (WUS), KNAT6, LATERAL ORGAN BOUNDARIES DOMAIN PROTEIN 1(LBD1), and BELL-like homeodomain protein 1 (BLH1), as well as tomato axillary meristem genes BLIND (Bl) and LATERAL SUPPRESSOR (Ls). More interestingly, the homologs of WUS and the potential functional partner OVATE FAMILIY PROTEIN (OFP) were subsequently down regulated during abscission while Bl and AGL12 were continuously and specifically induced in the abscission zone. The expression patterns of meristem activity genes corroborate the idea that cells of the abscission zone confer meristem-like nature and coincide with the course of abscission and post-abscission cell differentiation. Our data therefore propose a possible regulatory scheme in tomato involving meristem genes that may be required not only for the abscission zone development, but also for abscission. PMID:23390523

  14. Transcriptome analysis of tomato flower pedicel tissues reveals abscission zone-specific modulation of key meristem activity genes.

    PubMed

    Wang, Xiang; Liu, Danmei; Li, Aili; Sun, Xiuli; Zhang, Rongzhi; Wu, Liang; Liang, Yanchun; Mao, Long

    2013-01-01

    Tomato flower abscises at the anatomically distinct abscission zone that separates the pedicel into basal and apical portions. During abscission, cell separation occurs only at the abscission zone indicating distinctive molecular regulation in its cells. We conducted a transcriptome analysis of tomato pedicel tissues during ethylene promoted abscission. We found that the abscission zone was the most active site with the largest set of differentially expressed genes when compared with basal and apical portions. Gene Ontology analyses revealed enriched transcription regulation and hydrolase activities in the abscission zone. We also demonstrate coordinated responses of hormone and cell wall related genes. Besides, a number of ESTs representing homologs of key Arabidopsis shoot apical meristem activity genes were found to be preferentially expressed in the abscission zone, including WUSCHEL (WUS), KNAT6, LATERAL ORGAN BOUNDARIES DOMAIN PROTEIN 1(LBD1), and BELL-like homeodomain protein 1 (BLH1), as well as tomato axillary meristem genes BLIND (Bl) and LATERAL SUPPRESSOR (Ls). More interestingly, the homologs of WUS and the potential functional partner OVATE FAMILIY PROTEIN (OFP) were subsequently down regulated during abscission while Bl and AGL12 were continuously and specifically induced in the abscission zone. The expression patterns of meristem activity genes corroborate the idea that cells of the abscission zone confer meristem-like nature and coincide with the course of abscission and post-abscission cell differentiation. Our data therefore propose a possible regulatory scheme in tomato involving meristem genes that may be required not only for the abscission zone development, but also for abscission. PMID:23390523

  15. An attempt to monitor tectonic forces in the Vrancea active geodynamic zone: The Baspunar experiment

    NASA Astrophysics Data System (ADS)

    Besutiu, Lucian; Zlagnean, Luminita; Plopeanu, Marin

    2013-04-01

    (sparsely) run in the area, have provided inconsistent results on the PCF current dynamics. The Baspunar Geodynamic Observatory (BGO) has been designed and implemented by the Solid Earth Dynamics Department in the Institute of Geodynamics of the Romanian Academy in order to reveal and monitor eventual motions along PCF in the attempt to correlate variations in the slip rate with changes in the seismicity released within Vrancea zone. The first BGO records were strongly affected by changes in the atmospheric parameters. Consequently, technical measures and special corrections for the removal or at least mitigation of the effects created by changes in temperature, air pressure and humidity have been applied to the observations. In order to improve the signal to noise ratio, some mathematical filters have been applied too. The paper is aimed at revealing results of the geodetic observations along with preliminary geodynamic considerations. On the overall, after about two years of monitoring, PCF appears as an active tectonic contact. It mainly behaves as a left-lateral fault, but some short episodes with a reverse slip (dextral) were also pointed out. Correlations with crustal and intermediate-depth earthquakes occurring in both cases within the bending zone of East Carpathians are illustrated and discussed.

  16. Tank 241-AZ-102 SuperLig 639 Technetium Ion Exchange Eluate Evaporation Study

    SciTech Connect

    King, W.D.

    2001-02-15

    As part of the Hanford River Protection Project (RPP), the Savannah River Technology Center (SRTC) has conducted tests on the pretreatment and vitrification of a radioactive waste sample from Hanford Tank 241-AZ-102. The original, AZ-102 sample which was received at SRTC was characterized and filtered to remove entrained solids.1 The sample was then passed sequentially through ion exchange columns containing SuperLig{reg_sign} 644 and 639 resins for the removal of cesium and technetium ions (Tc removed as pertechnetate, TcO{sub 4}{sup {minus}}), respectively.2 The cesium and technetium absorbed to the resins was then eluted to give separate eluate solutions containing relatively high concentrations of Cs{sup +} and TcO{sub 4}{sup {minus}}. According to the current plant design, the decontaminated Tank 241-AZ-102 sample and the eluate solutions will be subjected to separate evaporation and vitrification processes to give low- and high-activity waste glasses, respectively. This report describes evaporation testing of the Tc eluate solution derived from ion exchange processing of the Tank 241-AZ-102 sample with SuperLig 639 resin.

  17. EDITORIAL: Adaptive and active materials: Selected papers from the ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems (SMASIS 11) (Scottsdale, AZ, USA, 18-21 September 2011) Adaptive and active materials: Selected papers from the ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems (SMASIS 11) (Scottsdale, AZ, USA, 18-21 September 2011)

    NASA Astrophysics Data System (ADS)

    Brei, Diann

    2012-09-01

    The fourth annual meeting of the ASME/AIAA Smart Materials, Adaptive Structures and Intelligent Systems Conference (SMASIS) took place in sunny Scottsdale, Arizona. Each year we strive to grow and offer new experiences. This year we held a special Guest Symposium on Sustainability along with two focused topic tracks on energy harvesting and active composites to encourage cross-fertilization between these important fields and our community. This cross-disciplinary emphasis was reflected in keynote talks by Dr Wayne Brown, President and founder of Dynalloy, Inc., 'Cross-Discipline Sharing'; Dr Brad Allenby, Arizona State University, 'You Want the Future? You can't Handle the Future!'; and Professor Aditi Chattopadhyay, Arizona State University, 'A Multidisciplinary Approach to Structural Health Monitoring and Prognosis'. SMASIS continues to grow our community through both social and technical interchange. The conference location, the exotic Firesky Resort and Spa, exemplified the theme of our Guest Symposium on Sustainability, being the only Green Seal certified resort in Arizona, and highlighting four elements thought to represent all that exist: fire, water, earth and air. Several special events were held around this theme including the night at the oasis reception sponsored by General Motors, sustainability bingo, smart trivia and student networking lunches, and an Arizona pow-wow with a spectacular Indian hoop dance. Our student and young professional development continues to grow strong with best paper and hardware competitions, scavenger student outing and games night. We are very proud that our students and young professionals are always seeking out ways to give back to the community, including organizing outreach to local high school talent. We thank all of our sponsors who made these special events possible. We hope that these social events provided participants with the opportunity to expand their own personal community and broaden their horizons. Our

  18. Diabatic heating profiles over the continental convergence zone during the monsoon active spells

    NASA Astrophysics Data System (ADS)

    Chattopadhyay, Rajib; Sur, Sharmila; Joseph, Susmitha; Sahai, A. K.

    2013-07-01

    The present paper aims to bring out the robust common aspects of spatio-temporal evolution of diabatic heating during the monsoon intraseasonal active phases over the continental tropical convergence zone (CTCZ). The robustness of spatio-temporal features is determined by comparing the two state-of-the art reanalyses: NCEP Climate Forecast System reanalysis and Modern ERA Retrospective Analysis. The inter-comparison is based on a study period of 26 years (1984-2009). The study confirms the development of deep heating over the CTCZ region during the active phase and is consistent between the two datasets. However, the detailed temporal evolution of the vertical structure (e.g., vertical tilts) of heating differs at times. The most important common feature from both the datasets is the significant vertical redistribution of heating with the development of shallow (low level) heating and circulation over the CTCZ region 3-7 days after the peak active phase. The shallow circulation is found to be associated with increased vertical shear and relative vorticity over certain regions in the subcontinent. This increased vertical shear and relative vorticity in the lower levels could be crucial in the sustenance of rainfall after the peak active phase. Model experiments with linear dynamics affirm the role of shallow convection in increasing the lower level circulation as observed.

  19. Assessment of the biological activity of soils in the subtropical zone of Azerbaijan

    NASA Astrophysics Data System (ADS)

    Babaev, M. P.; Orujova, N. I.

    2009-10-01

    The enzymatic activity; the microbial population; and the intensities of the nitrification, ammonification, CO2emission, and cellulose decomposition were studied in gray-brown, meadow-sierozemic, meadow-forest alluvial, and yellow (zheltozem) gley soils in the subtropical zone of Azerbaijan under natural vegetation, crop rotation systems with vegetables, and permanent vegetable crops. On this basis, the biological diagnostics of these soils were suggested and the soil ecological health was evaluated. It was shown that properly chosen crop rotation systems on irrigated lands make it possible to preserve the fertility of the meadow-forest alluvial and zheltozem-gley soils and to improve the fertility of the gray-brown and meadow-sierozemic soils.

  20. Nanoscale dynamics of synaptic vesicle trafficking and fusion at the presynaptic active zone

    PubMed Central

    Vaithianathan, Thirumalini; Henry, Diane; Akmentin, Wendy; Matthews, Gary

    2016-01-01

    The cytomatrix at the active zone (CAZ) is a macromolecular complex that facilitates the supply of release-ready synaptic vesicles to support neurotransmitter release at synapses. To reveal the dynamics of this supply process in living synapses, we used super-resolution imaging to track single vesicles at voltage-clamped presynaptic terminals of retinal bipolar neurons, whose CAZ contains a specialized structure—the synaptic ribbon—that supports both fast, transient and slow, sustained modes of transmission. We find that the synaptic ribbon serves a dual function as a conduit for diffusion of synaptic vesicles and a platform for vesicles to fuse distal to the plasma membrane itself, via compound fusion. The combination of these functions allows the ribbon-type CAZ to achieve the continuous transmitter release required by synapses of neurons that carry tonic, graded visual signals in the retina. DOI: http://dx.doi.org/10.7554/eLife.13245.001 PMID:26880547

  1. Seismic evidence for active underplating below the megathrust earthquake zone in Japan.

    PubMed

    Kimura, Hisanori; Takeda, Tetsuya; Obara, Kazushige; Kasahara, Keiji

    2010-07-01

    Determining the structure of subduction zones is important for understanding mechanisms for the generation of interplate phenomena such as megathrust earthquakes. The peeling off of the uppermost part of a subducting slab and accretion to the bottom of an overlying plate (underplating) at deep regions has been inferred from exhumed metamorphic rocks and deep seismic imaging, but direct seismic evidence of this process is lacking. By comparing seismic reflection profiles with microearthquake distributions in central Japan, we show that repeating microearthquakes occur along the bottom interface of the layer peeling off from the subducting Philippine Sea plate. This region coincides with the location of slow-slip events that may serve as signals for monitoring active underplating. PMID:20616277

  2. Growth of the active zone in nitride based long wavelength laser structures

    NASA Astrophysics Data System (ADS)

    Rossow, U.; Jönen, H.; Brendel, M.; Dräger, A.; Langer, T.; Hoffmann, L.; Bremers, H.; Hangleiter, A.

    2011-01-01

    In xGa 1- xN/GaN quantum well (QW) structures grown on c-plane surfaces for long wavelength light emitters have been investigated intended. We reached indium concentrations of xIn≥0.35 with good optical and structural quality. For QW thicknesses dQW≤2 nm a fully strained layer structure is observed. QWs of such high indium concentrations, however, are very sensitive to the growth conditions of the subsequent layers and thermal stability/degradation becomes an important issue. We modified the growth of the QWs to avoid or minimize V-pit formation without temperature ramping in the barriers and showed that their properties were unchanged when used in the active zone of a laser structure.

  3. Determination of dissociation constants of pharmacologically active xanthones by capillary zone electrophoresis with diode array detection.

    PubMed

    Wu, Xiaomu; Gong, Suxuan; Bo, Tao; Liao, Yiping; Liu, Huwei

    2004-12-24

    In this article, the dissociation constants (pKa) of 10 pharmacologically active xanthones isolated from herbal medicine Securidaca inappendiculata were determined by capillary zone electrophoresis with diode array detection. The pKa values determined by the method based on the electrophoretic mobilities (calculated from migration times) have been proved by the method based on UV absorbance calculated from the online spectra corresponding peaks. No conspicuous difference was observed between the two methods with acceptable reproducibility. Two pKa values (pKa1 and pKa2) were found for four xanthones while generally the 10 compounds possess the pKa values ranging from 6.4 to 9.2. PMID:15641365

  4. A cellular automaton model for microstructural simulation of friction stir welded AZ91 magnesium alloy

    NASA Astrophysics Data System (ADS)

    Akbari, Mostafa; Asadi, Parviz; Besharati Givi, MohammadKazem; Zolghadr, Parisa

    2016-03-01

    To predict the grain size and microstructure evolution during friction stir welding (FSW) of AZ91 magnesium alloy, a finite element model (FEM) is developed based on the combination of a cellular automaton model and the Kocks  -  Mecking and Laasraoui-Jonas models. First, according to the flow stress curves and using the Kocks  -  Mecking model, the hardening and recovery parameters and the strain rate sensitivity were calculated. Next, an FEM model was established in Deform-3D software to simulate the FSW of AZ91 magnesium alloy. The results of the FEM model are used in microstructure evolution models to predict the grain size and microstructure of the weld zone. There is a good agreement between the simulated and experimental microstructures, and the proposed model can simulate the dynamic recrystallization (DRX) process during FSW of AZ91 alloy. Moreover, microstructural properties of different points in the SZ as well as the effect of the w/v parameter on the grain size and microstructure are considered.

  5. 78 FR 45181 - Foreign-Trade Zone 230-Piedmont Triad Area, North Carolina, Authorization of Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-26

    ... inviting public comment (78 FR 23220, 4-18-2013). The FTZ Board has determined that no further review of... Production Activity, Oracle Flexible Packaging, Inc., (Foil-Backed Paperboard), Winston-Salem, North Carolina... proposed production activity to the Foreign-Trade Zones (FTZ) Board on behalf of Oracle Flexible...

  6. APP Is a Context-Sensitive Regulator of the Hippocampal Presynaptic Active Zone.

    PubMed

    Laßek, Melanie; Weingarten, Jens; Wegner, Martin; Mueller, Benjamin F; Rohmer, Marion; Baeumlisberger, Dominic; Arrey, Tabiwang N; Hick, Meike; Ackermann, Jörg; Acker-Palmer, Amparo; Koch, Ina; Müller, Ulrike; Karas, Michael; Volknandt, Walter

    2016-04-01

    The hallmarks of Alzheimer's disease (AD) are characterized by cognitive decline and behavioral changes. The most prominent brain region affected by the progression of AD is the hippocampal formation. The pathogenesis involves a successive loss of hippocampal neurons accompanied by a decline in learning and memory consolidation mainly attributed to an accumulation of senile plaques. The amyloid precursor protein (APP) has been identified as precursor of Aβ-peptides, the main constituents of senile plaques. Until now, little is known about the physiological function of APP within the central nervous system. The allocation of APP to the proteome of the highly dynamic presynaptic active zone (PAZ) highlights APP as a yet unknown player in neuronal communication and signaling. In this study, we analyze the impact of APP deletion on the hippocampal PAZ proteome. The native hippocampal PAZ derived from APP mouse mutants (APP-KOs and NexCreAPP/APLP2-cDKOs) was isolated by subcellular fractionation and immunopurification. Subsequently, an isobaric labeling was performed using TMT6 for protein identification and quantification by high-resolution mass spectrometry. We combine bioinformatics tools and biochemical approaches to address the proteomics dataset and to understand the role of individual proteins. The impact of APP deletion on the hippocampal PAZ proteome was visualized by creating protein-protein interaction (PPI) networks that incorporated APP into the synaptic vesicle cycle, cytoskeletal organization, and calcium-homeostasis. The combination of subcellular fractionation, immunopurification, proteomic analysis, and bioinformatics allowed us to identify APP as structural and functional regulator in a context-sensitive manner within the hippocampal active zone network. PMID:27092780

  7. Quaternary grabens in southernmost Illinois: Deformation near an active intraplate seismic zone

    USGS Publications Warehouse

    Nelson, W.J.; Denny, F.B.; Follmer, L.R.; Masters, J.M.

    1999-01-01

    Narrow grabens displace Quaternary sediments near the northern edge of the Mississippi Embayment in extreme southern Illinois, east-central United States. Grabens are part of the Fluorspar Area Fault Complex (FAFC), which has been recurrently active throughout Phanerozoic time. The FAFC strikes directly toward the New Madrid Seismic Zone (NMSZ), scene of some of the largest intra-plate earthquakes in history. The NMSZ and FAFC share origin in a failed Cambrian rift (Reelfoot Rift). Every major fault zone of the FAFC in Illinois exhibits Quaternary displacement. The structures appear to be strike-slip pull-apart grabens, but the magnitude and direction of horizontal slip and their relationship to the current stress field are unknown. Upper Tertiary strata are vertically displaced more than 100 m, Illinoian and older Pleistocene strata 10 to 30 m, and Wisconsinan deposits 1 m or less. No Holocene deformation has been observed. Average vertical slip rates are estimated at 0.01 to 0.03 mm/year, and recurrence intervals for earthquakes of magnitude 6 to 7 are on the order of 10,000s of years for any given fault. Previous authors remarked that the small amount of surface deformation in the New Madrid area implies that the NMSZ is a young feature. Our findings show that tectonic activity has shifted around throughout the Quaternary in the central Mississippi Valley. In addition to the NMSZ and southern Illinois, the Wabash Valley (Illinois-Indiana), Benton Hills (Missouri), Crowley's Ridge (Arkansas-Missouri), and possibly other sites have experienced Quaternary tectonism. The NMSZ may be only the latest manifestation of seismicity in an intensely fractured intra-plate region.

  8. The property of fault zone and fault activity of Shionohira Fault, Fukushima, Japan

    NASA Astrophysics Data System (ADS)

    Seshimo, K.; Aoki, K.; Tanaka, Y.; Niwa, M.; Kametaka, M.; Sakai, T.; Tanaka, Y.

    2015-12-01

    The April 11, 2011 Fukushima-ken Hamadori Earthquake (hereafter the 4.11 earthquake) formed co-seismic surface ruptures trending in the NNW-SSE direction in Iwaki City, Fukushima Prefecture, which were newly named as the Shionohira Fault by Ishiyama et al. (2011). This earthquake was characterized by a westward dipping normal slip faulting, with a maximum displacement of about 2 m (e.g., Kurosawa et al., 2012). To the south of the area, the same trending lineaments were recognized to exist even though no surface ruptures occurred by the earthquake. In an attempt to elucidate the differences of active and non-active segments of the fault, this report discusses the results of observation of fault outcrops along the Shionohira Fault as well as the Coulomb stress calculations. Only a few outcrops have basement rocks of both the hanging-wall and foot-wall of the fault plane. Three of these outcrops (Kyodo-gawa, Shionohira and Betto) were selected for investigation. In addition, a fault outcrop (Nameishi-minami) located about 300 m south of the southern tip of the surface ruptures was investigated. The authors carried out observations of outcrops, polished slabs and thin sections, and performed X-ray diffraction (XRD) to fault materials. As a result, the fault zones originating from schists were investigated at Kyodo-gawa and Betto. A thick fault gouge was cut by a fault plane of the 4.11 earthquake in each outcrop. The fault materials originating from schists were fault bounded with (possibly Neogene) weakly deformed sandstone at Shionohira. A thin fault gouge was found along the fault plane of 4.11 earthquake. A small-scale fault zone with thin fault gouge was observed in Nameishi-minami. According to XRD analysis, smectite was detected in the gouges from Kyodo-gawa, Shionohira and Betto, while not in the gouge from Nameishi-minami.

  9. APP Is a Context-Sensitive Regulator of the Hippocampal Presynaptic Active Zone

    PubMed Central

    Mueller, Benjamin F.; Rohmer, Marion; Baeumlisberger, Dominic; Arrey, Tabiwang N.; Hick, Meike; Ackermann, Jörg; Acker-Palmer, Amparo; Koch, Ina; Müller, Ulrike; Karas, Michael; Volknandt, Walter

    2016-01-01

    The hallmarks of Alzheimer’s disease (AD) are characterized by cognitive decline and behavioral changes. The most prominent brain region affected by the progression of AD is the hippocampal formation. The pathogenesis involves a successive loss of hippocampal neurons accompanied by a decline in learning and memory consolidation mainly attributed to an accumulation of senile plaques. The amyloid precursor protein (APP) has been identified as precursor of Aβ-peptides, the main constituents of senile plaques. Until now, little is known about the physiological function of APP within the central nervous system. The allocation of APP to the proteome of the highly dynamic presynaptic active zone (PAZ) highlights APP as a yet unknown player in neuronal communication and signaling. In this study, we analyze the impact of APP deletion on the hippocampal PAZ proteome. The native hippocampal PAZ derived from APP mouse mutants (APP-KOs and NexCreAPP/APLP2-cDKOs) was isolated by subcellular fractionation and immunopurification. Subsequently, an isobaric labeling was performed using TMT6 for protein identification and quantification by high-resolution mass spectrometry. We combine bioinformatics tools and biochemical approaches to address the proteomics dataset and to understand the role of individual proteins. The impact of APP deletion on the hippocampal PAZ proteome was visualized by creating protein-protein interaction (PPI) networks that incorporated APP into the synaptic vesicle cycle, cytoskeletal organization, and calcium-homeostasis. The combination of subcellular fractionation, immunopurification, proteomic analysis, and bioinformatics allowed us to identify APP as structural and functional regulator in a context-sensitive manner within the hippocampal active zone network. PMID:27092780

  10. The River Network, Active Tectonics and the Mexican Subduction Zone, Southwest Mexico

    NASA Astrophysics Data System (ADS)

    Gaidzik, K.; Ramirez-Herrera, M. T.; Kostoglodov, V.; Basili, R.

    2014-12-01

    Rivers, their profiles and network reflect the integration of multiple processes and forces that are part of the fundamental controls on the relief structure of mountain belts. The motivation of this study is to understand active tectonic processes in the forearc region of subduction zones, by distinguishing evidence of active deformation using the river network and topography. To this end, morphotectonic and structural studies have been conducted on fifteen drainage basins on the mountain front, parallel to the Mexican subduction zone, where the Cocos plate underthrusts the North American plate. The southwest - northeast Cocos plate subduction stress regime initiated ca. 20 MA. NE-SW to NNE-SSW normal faults as well as sub-latitudinal to NW-SE strike-slip faults (both dextral and sinistral) constitute the majority of mesofaults recorded in the field within the studied drainage basins. Occasionally dextral N-S strike-slip faults also occur. The stress tensor reconstruction suggests two main evolution stages of these faults: 1) the older is dominated by a NW-SE to WNW-ESE extensional regime and 2) the younger is a transcurrent regime, with NNE-SSW σ1 axis. The drainage pattern is strongly controlled by tectonic features, whereas lithology is only a subordinate factor, with only one exception (Petatlán river). Generally, major rivers flow from north to south mainly through NE-SW and NNE-SSW normal faults, and/or sub-longitudinal dextral (also locally sinistral) strike-slip faults. In the central and eastern part of the studied area, rivers also follow NW-SE structures, which are generally normal or sinistral strike-slip faults (rarely reverse). In most cases, local deflections of the river main courses are related to sub-latitudinal strike-slip faults, both dextral and sinistral. Within the current stress field related to the active Cocos subduction, both normal and strike-slip fault sets could be reactivated. Our analysis suggests that strike-slip faults, mainly

  11. Analysis of protein phosphorylation in nerve terminal reveals extensive changes in active zone proteins upon exocytosis

    PubMed Central

    Kohansal-Nodehi, Mahdokht; Chua, John JE; Urlaub, Henning; Jahn, Reinhard; Czernik, Dominika

    2016-01-01

    Neurotransmitter release is mediated by the fast, calcium-triggered fusion of synaptic vesicles with the presynaptic plasma membrane, followed by endocytosis and recycling of the membrane of synaptic vesicles. While many of the proteins governing these processes are known, their regulation is only beginning to be understood. Here we have applied quantitative phosphoproteomics to identify changes in phosphorylation status of presynaptic proteins in resting and stimulated nerve terminals isolated from the brains of Wistar rats. Using rigorous quantification, we identified 252 phosphosites that are either up- or downregulated upon triggering calcium-dependent exocytosis. Particularly pronounced were regulated changes of phosphosites within protein constituents of the presynaptic active zone, including bassoon, piccolo, and RIM1. Additionally, we have mapped kinases and phosphatases that are activated upon stimulation. Overall, our study provides a snapshot of phosphorylation changes associated with presynaptic activity and provides a foundation for further functional analysis of key phosphosites involved in presynaptic plasticity. DOI: http://dx.doi.org/10.7554/eLife.14530.001 PMID:27115346

  12. 241-AZ Farm Annulus Extent of Condition Baseline Inspection

    SciTech Connect

    Engeman, Jason K.; Girardot, Crystal L.; Vazquez, Brandon J.

    2013-05-15

    This report provides the results of the comprehensive annulus visual inspection for tanks 241- AZ-101 and 241-AZ-102 performed in fiscal year 2013. The inspection established a baseline covering about 95 percent of the annulus floor for comparison with future inspections. Any changes in the condition are also included in this document.

  13. ANALYSIS RESULTS FOR BUILDING 241 702-AZ A TRAIN

    SciTech Connect

    DUNCAN JB; FRYE JM; COOKE CA; LI SW; BROCKMAN FJ

    2006-12-13

    This report presents the analyses results for three samples obtained under RPP-PLAN-28509, Sampling and Analysis Plan for Building 241 702-AZ A Train. The sampling and analysis was done in response to problem evaluation request number PER-2004-6139, 702-AZ Filter Rooms Need Radiological Cleanup Efforts.

  14. Gas emissions and active tectonics within the submerged section of the North Anatolian Fault zone in the Sea of Marmara

    NASA Astrophysics Data System (ADS)

    Géli, L.; Henry, P.; Zitter, T.; Dupré, S.; Tryon, M.; Çağatay, M. N.; de Lépinay, B. Mercier; Le Pichon, X.; Şengör, A. M. C.; Görür, N.; Natalin, B.; Uçarkuş, G.; Özeren, S.; Volker, D.; Gasperini, L.; Burnard, P.; Bourlange, S.; Marnaut Scientific Party

    2008-09-01

    The submerged section of the North Anatolian fault within the Marmara Sea was investigated using acoustic techniques and submersible dives. Most gas emissions in the water column were found near the surface expression of known active faults. Gas emissions are unevenly distributed. The linear fault segment crossing the Central High and forming a seismic gap - as it has not ruptured since 1766, based on historical seismicity, exhibits relatively less gas emissions than the adjacent segments. In the eastern Sea of Marmara, active gas emissions are also found above a buried transtensional fault zone, which displayed micro-seismic activity after the 1999 events. Remarkably, this zone of gas emission extends westward all along the southern edge of Cinarcik basin, well beyond the zone where 1999 aftershocks were observed. The long term monitoring of gas seeps could hence be highly valuable for the understanding of the evolution of the fluid-fault coupling processes during the earthquake cycle within the Marmara Sea.

  15. Probing Microbial Activity in a Perched Water Body Located in a Deep Vadose Zone

    NASA Astrophysics Data System (ADS)

    Fujita, Y.; Taylor, J. L.; Henriksen, J. R.; Delwiche, M.; Gebrehiwet, T.; Hubbard, S. S.; Spycher, N.; Weathers, T. S.; Ginn, T. R.; Pfiffner, S. M.; Smith, R. W.

    2011-12-01

    Waste releases to the vadose zone are a legacy of past activities at a number of Department of Energy (DOE) facilities. At the Idaho National Laboratory (INL), 90Sr has been detected in perched water bodies underlying the Idaho Nuclear Technology and Engineering Center (INTEC) facility. Microbially induced calcite precipitation (MICP) using urea-hydrolyzing microbes is one proposed approach for immobilization of 90Sr in the subsurface. The sequestration mechanism is co-precipitation in calcite, promoted by the production of carbonate alkalinity from ureolysis. In order to assess the potential efficacy of MICP at INTEC a field study was conducted at the INL Vadose Zone Research Park (VZRP). The VZRP is located approximately 3 km from INTEC and shares many of the same hydrologic and lithologic features but in a non-contaminated setting. We conducted experiments over two field seasons in a perched water body located approximately 15 meters below land surface, using a 5-spot wellfield design. During the first season amendments (molasses and urea) were injected into the central well and water was extracted from two wells on either side, located along a diagonal. Water samples were characterized for microbial abundance, ureolytic activity and ureC gene numbers, along with solution composition. Before, during and after the injections cross-borehole geophysical imaging was performed, using various combinations of the available wells. During the second field season in situ static experiments were conducted to specifically characterize attached and unattached microbial communities, using surrogate substrates colonized during a 12 week incubation. Based on the field data a first order in situ urea hydrolysis rate constant of 0.034 d-1 was estimated. This was more than an order of magnitude higher than rate constants estimated above-ground using water samples, suggesting that attached microorganisms were responsible for >90% of the observed urea hydrolysis activity. The

  16. Probabilistic secretion of quanta and the synaptosecretosome hypothesis: evoked release at active zones of varicosities, boutons, and endplates.

    PubMed Central

    Bennett, M R; Gibson, W G; Robinson, J

    1997-01-01

    A quantum of transmitter may be released upon the arrival of a nerve impulse if the influx of calcium ions through a nearby voltage-dependent calcium channel is sufficient to activate the vesicle-associated calcium sensor protein that triggers exocytosis. A synaptic vesicle, together with its calcium sensor protein, is often found complexed with the calcium channel in active zones to form what will be called a "synaptosecretosome." In the present work, a stochastic analysis is given of the conditions under which a quantum is released from the synaptosecretosome by a nerve impulse. The theoretical treatment considers the rise of calcium at the synaptosecretosome after the stochastic opening of a calcium channel at some time during the impulse, followed by the stochastic binding of calcium to the vesicle-associated protein and the probability of this leading to exocytosis. This allows determination of the probabilities that an impulse will release 0, 1, 2,... quanta from an active zone, whether this is in a varicosity, a bouton, or a motor endplate. A number of experimental observations of the release of transmitter at the active zones of sympathetic varicosities and boutons as well as somatic motor endplates are described by this analysis. These include the likelihood of the secretion of only one quantum at an active zone of endplates and of more than one quantum at an active zone of a sympathetic varicosity. The fourth-power relationship between the probability of transmitter release at the active zones of sympathetic varicosities and motor endplates and the external calcium concentration is also explained by this approach. So, too, is the fact that the time course of the increased rate of quantal secretion from a somatic active zone after an impulse is invariant with changes in the amount of calcium that enters through its calcium channel, whether due to changes consequent on the actions of autoreceptor agents such as adenosine or to facilitation. The increased

  17. Observations of Seafloor Deformation and Methane Venting within an Active Fault Zone Offshore Southern California

    NASA Astrophysics Data System (ADS)

    Anderson, K.; Lundsten, E. M.; Paull, C. K.; Caress, D. W.; Thomas, H. J.; Brewer, P. G.; Vrijenhoek, R.; Lundsten, L.

    2013-12-01

    Detailed mapping surveys of the floor and flanks of the Santa Monica Basin, San Pedro Basin, and San Diego Trough were conducted during the past seven years using an Autonomous Underwater Vehicle (AUV) built and operated by MBARI specifically for seafloor mapping. The AUV collected data provide up to 1 m resolution multibeam bathymetric grids with a vertical precision of 0.15 m. Along with high-resolution multibeam, the AUV also collects chirp seismic reflection profiles. Structures within the uppermost 10-20 m of the seafloor, which in the surveys presented here is composed of recent sediment drape, can typically be resolved in the sub-bottom reflectors. Remotely operated vehicle (ROV) dives allowed for ground-truth observations and sampling within the surveyed areas. The objectives of these dives included finding evidence of recent seafloor deformation and locating areas where chemosynthetic biological communities are supported by fluid venting. Distinctive seafloor features within an active fault zone are revealed in unprecedented detail in the AUV generated maps and seismic reflection profiles. Evidence for recent fault displacements include linear scarps which can be as small as 20 cm high but traceable for several km, right lateral offsets within submarine channels and topographic ridges, and abrupt discontinuities in sub-bottom reflectors, which in places appear to displace seafloor sediments. Several topographic highs that occur within the fault zone appear to be anticlines related to step-overs in these faults. These topographic highs are, in places, topped with circular mounds that are up to 15 m high and have ~30° sloping sides. The crests of the topographic highs and the mounds both have distinctive rough morphologies produced by broken pavements of irregular blocks of methane-derived authigenic carbonates, and by topographic depressions, commonly more than 2 m deep. These areas of distinctive rough topography are commonly associated with living

  18. Impaired hippocampal activity at the goal zone on the place preference task in a DISC1 mouse model.

    PubMed

    Hayashi, Yuichiro; Sawa, Akira; Hikida, Takatoshi

    2016-05-01

    Learning deficit is a clinical feature of many mental disorders and is hypothesized to result from an inability to integrate information in neural systems. We showed that transgenic mice expressing a dominant-negative form of DISC1, a risk gene for neuropsychiatric disorders, exhibited impaired performance in a reward-place association task when combined with a mild isolation stress. CA1 cells in the mutant mice showed normal place cell properties, but their activity at the goal zone was diminished. This abnormality in hippocampal activity at the goal zone during the task may underlie the learning deficit observed in the DISC1 mutant mice. PMID:26497623

  19. Locating an active fault zone in Coso geothermal field by analyzing seismic guided waves from microearthquake data

    SciTech Connect

    SGP-TR-150-16

    1995-01-26

    Active fault systems usually provide high-permeability channels for hydrothermal outflow in geothermal fields. Locating such fault systems is of a vital importance to plan geothermal production and injection drilling, since an active fault zone often acts as a fracture-extensive low-velocity wave guide to seismic waves. We have located an active fault zone in the Coso geothermal field, California, by identifying and analyzing a fault-zone trapped Rayleigh-type guided wave from microearthquake data. The wavelet transform is employed to characterize guided-wave's velocity-frequency dispersion, and numerical methods are used to simulate the guided-wave propagation. The modeling calculation suggests that the fault zone is {approx} 200m wide, and has a P wave velocity of 4.80 km/s and a S wave velocity of 3.00 km/s, which is sandwiched between two half spaces with relatively higher velocities (P wave velocity 5.60 km/s, and S wave velocity 3.20 km/s). zones having vertical or nearly vertical dipping fault planes.

  20. Peculiarities of ULF electromagnetic disturbances before strong earthquakes in seismic active zone of Kamchatka peninsula

    NASA Astrophysics Data System (ADS)

    Kopytenko, Y. A.; Ismagilov, V. S.; Schekotov, A.; Molchanov, O.; Chebrov, V.; Raspopov, O. M.

    2006-12-01

    Regular observations of ULF electromagnetic disturbances and acoustic emissions at st. Karymshino in seismic active zone of Kamchatka peninsula were carried out during 2001-2003 years. Five seismic active periods with strong earthquakes (M>5) were displayed during this period. These EQs occurred at the Pacific at 20-60 km depth at 100-140 km distances to the East from the st. Karymshino. Analysis of normalized dynamic power spectra of data of high-sensitive (0.2 pT/sqrt(Hz)) three-component induction magnetometer achieved a significant disorder of daily variation and increasing of the magnetic disturbance intensities (from 0.2 to ~1 pT) in the whole investigated frequency range (0.2-5 Hz). The anomaly intensity increasing was observed during the 12-18 hours before main seismic shocks. Maximum of the increasing occurred during 4-6 hours before the EQs. An increasing of acoustic emissions (F=30 Hz) was observed during the same period. A sharp decreasing of the magnetic disturbance intensities was observed 2-4 hours before the EQs. We suppose that physical processes in a hearth of forthcoming EQ lead to an irreversible avalanche-like formation of cracks and stimulation of the acoustic and ULF electromagnetic disturbances.

  1. Distribution of dehalogenation activity in subseafloor sediments of the Nankai Trough subduction zone

    PubMed Central

    Futagami, Taiki; Morono, Yuki; Terada, Takeshi; Kaksonen, Anna H.; Inagaki, Fumio

    2013-01-01

    Halogenated organic matter buried in marine subsurface sediment may serve as a source of electron acceptors for anaerobic respiration of subseafloor microbes. Detection of a diverse array of reductive dehalogenase-homologous (rdhA) genes suggests that subseafloor organohalide-respiring microbial communities may play significant ecological roles in the biogeochemical carbon and halogen cycle in the subseafloor biosphere. We report here the spatial distribution of dehalogenation activity in the Nankai Trough plate-subduction zone of the northwest Pacific off the Kii Peninsula of Japan. Incubation experiments with slurries of sediment collected at various depths and locations showed that degradation of several organohalides tested only occurred in the shallow sedimentary basin, down to 4.7 metres below the seafloor, despite detection of rdhA in the deeper sediments. We studied the phylogenetic diversity of the metabolically active microbes in positive enrichment cultures by extracting RNA, and found that Desulfuromonadales bacteria predominate. In addition, for the isolation of genes involved in the dehalogenation reaction, we performed a substrate-induced gene expression screening on DNA extracted from the enrichment cultures. Diverse DNA fragments were obtained and some of them showed best BLAST hit to known organohalide respirers such as Dehalococcoides, whereas no functionally known dehalogenation-related genes such as rdhA were found, indicating the need to improve the molecular approach to assess functional genes for organohalide respiration. PMID:23479745

  2. An automatic continuous monitoring station for groundwater geochemistry at an active fault zone in SW Taiwan

    NASA Astrophysics Data System (ADS)

    Lai, Chun-Wei; Yang, Tsanyao F.; Fu, Ching-Chou; Hilton, David R.; Liu, Tsung-Kwei; Walia, Vivek; Lai, Tzu-Hua

    2015-04-01

    Previous studies have revealed that gas compositions of fluid samples collected from southwestern Taiwan where many hot springs and mud volcanoes are distributed along tectonic sutures show significant variation prior to and after some disaster seismic events. Such variations, including radon activity, CH4/CO2, CO2/3He and 3He/4He ratios of gas compositions, are considered to be precursors of earthquakes in this area. To validate the relationship between fluid compositions and local earthquakes, a continuous monitoring station has been established at Yun-Shui, which is an artesian well located at an active fault zone in SW Taiwan. It is equipped with a radon detector and a quadrupole mass spectrometer (QMS) for in-situ measurement of the dissolved gas composition. Data is telemetered to Taipei so we are able to monitor variations of gas composition in real time. Furthermore, we also installed a syringe pump apparatus for the retrieval and temporal analysis of helium (SPARTAH) at this station. From the SPARTAH samples, we can obtain detailed time series records of H-O isotopic compositions, DIC concentration and δ13C isotopic ratios, and anion concentration of the water samples at this station. After continuous monitoring for about one year, some anomalies occurred prior to some local earthquakes. It demonstrates that this automated system is feasible for long-term continuous seismo-geochemical research in this area. Keywords: monitoring; geochemistry; isotope; dissolved gases; pre-seismic signal.

  3. Slip Rates of Main Active Fault Zones Through Turkey Inferred From GPS Observations

    NASA Astrophysics Data System (ADS)

    Ozener, H.; Aktug, B.; Dogru, A.; Tasci, L.; Acar, M.; Emre, O.; Yilmaz, O.; Turgut, B.; Halicioglu, K.; Sabuncu, A.; Bal, O.; Eraslan, A.

    2015-12-01

    Active Fault Map of Turkey was revised and published by General Directorate of Mineral Research and Exploration in 2012. This map reveals that there are about 500 faults can generate earthquakes.In order to understand the earthquake potential of these faults, it is needed to determine the slip rates. Although many regional and local studies were performed in the past, the slip rates of the active faults in Turkey have not been determined. In this study, the block modelling, which is the most common method to produce slip rates, will be done. GPS velocities required for block modeling is being compiled from the published studies and the raw data provided then velocity field is combined. To form a homogeneous velocity field, different stochastic models will be used and the optimal velocity field will be achieved. In literature, GPS site velocities, which are computed for different purposes and published, are combined globally and this combined velocity field are used in the analysis of strain accumulation. It is also aimed to develop optimal stochastic models to combine the velocity data. Real time, survey mode and published GPS observations is being combined in this study. We also perform new GPS observations. Furthermore, micro blocks and main fault zones from Active Fault Map Turkey will be determined and homogeneous velocity field will be used to infer slip rates of these active faults. Here, we present the result of first year of the study. This study is being supported by THE SCIENTIFIC AND TECHNOLOGICAL RESEARCH COUNCIL OF TURKEY (TUBITAK)-CAYDAG with grant no. 113Y430.

  4. New insights on the seismogenic potential of the Eastern Betic Shear Zone (SE Iberia): Quaternary activity and paleoseismicity of the SW segment of the Carrascoy Fault Zone

    NASA Astrophysics Data System (ADS)

    Martín-Banda, Raquel; García-Mayordomo, Julián.; Insua-Arévalo, Juan M.; Salazar, Ángel E.; Rodríguez-Escudero, Emilio; Álvarez-Gómez, Jose A.; Medialdea, Alicia; Herrero, María. J.

    2016-01-01

    The Carrascoy Fault (CAF) is one of the main active faults that form part of the Eastern Betic Shear Zone, a 450 km fault system that accommodates most of the convergence between the Eurasian (Iberia) and Nubian plates in the Betic Cordillera, south Spain. Although the CAF represents a major earthquake threat to the nearby City of Murcia, studies on its Quaternary tectonics and seismogenic potential are scarce to date. We present evidence that supports the division of the CAF into two overlapping segments with contrasting tectonic structure, Quaternary activity, and landform control: a SW segment, characterized by a broad fold-and-thrust zone similar to the forebergs defined in the Gobi-Altai region, and a NE segment, characterized by a sharp mountain front controlled by strike-slip tectonics. We attribute the differentiation into these two segments to the stresses associated with topography, which in turn is a consequence of the shortening component, at the middle Pleistocene, after circa 217.4 ka. For the SW segment we infer the occurrence of 9 to 11, Mw 6.7 paleoearthquakes in the last 30.2 kyr, and a slip rate of 0.37 ± 0.08 m/kyr. We date the occurrence of the last surface rupture event after 2750 B.P., and we estimate an average recurrence period of major events of 3.3 ± 0.7 kyr.

  5. Active deformation along the Andaman-Nicobar subduction zone from seismic reflection studies

    NASA Astrophysics Data System (ADS)

    Moeremans, R. E.; Singh, S. C.

    2013-12-01

    The Andaman-Sumatra subduction zone is one of the most seismically active regions on Earth and is a prime example of oblique subduction. It is the result of the oblique convergence between the downgoing Indo-Australian and the overriding Eurasian plates, leading to slip partitioning into a trench-normal thrust component along the plate interface and a trench-subparallel strike-slip component along a sliver fault. The direction of convergence is 90° with respect to the trench near Java, reduces to 45° off of northern Sumatra, and becomes almost parallel to the trench along the Andaman-Nicobar portion of the subduction. Rates of subduction vary from 63 mm/yr off of Java, 50 mm/yr near Nias Island, 45 mm/yr northwest of Sumatra, and 39 mm/yr near the Andaman Islands. After the great December 2004 earthquake, the Sumatran section of the subduction zone was heavily investigated using marine geophysical studies, but the deformation processes in the Andaman-Nicobar region remain poorly understood due to the lack of data. Here, we present seismic reflection profiles from the Andaman-Nicobar region that cover the deformation front, the forearc high, and the forearc basin. We find that the presence of thick (> 3 s TWT) sediments lead to slip taking place predominantly along landward vergent frontal faults. The frontal fault vergence changes to seaward due to the thinning (< 2 s TWT) of the sediments in the region where the Ninetyeast ridge subducts. The presence of a thick (> 3 s TWT) 20 km-long unit of undeformed sediments, possibly resulting from the landward vergence of the frontal thrusts, suggests that ~40 km of the Ninetyeast ridge has subducted beneath the Andaman forearc. The forearc is widest between the Andaman and Nicobar Islands, likely due to the subduction of thick sediments. The forearc basin is bounded in the west by a series of backthrusts and is underlain by a continental crust, which was once a part of the Malay Peninsula. The forearc basin is crescent

  6. Active tectonics west of New Zealand's Alpine Fault: South Westland Fault Zone activity shows Australian Plate instability

    NASA Astrophysics Data System (ADS)

    De Pascale, Gregory P.; Chandler-Yates, Nicholas; Dela Pena, Federico; Wilson, Pam; May, Elijah; Twiss, Amber; Cheng, Che

    2016-04-01

    The 300 km long South Westland Fault Zone (SWFZ) is within the footwall of the Central Alpine Fault (<20 km away) and has 3500 m of dip-slip displacement, but it has been unknown if the fault is active. Here the first evidence for SWFZ thrust faulting in the "stable" Australian Plate is shown with cumulative dip-slip displacements up to 5.9 m (with 3 m throw) on Pleistocene and Holocene sediments and gentle hanging wall anticlinal folding. Cone penetration test (CPT) stratigraphy shows repeated sequences within the fault scarp (consistent with thrusting). Optically stimulated luminescence (OSL) dating constrains the most recent rupture post-12.1 ± 1.7 ka with evidence for three to four events during earthquakes of at least Mw 6.8. This study shows significant deformation is accommodated on poorly characterized Australian Plate structures northwest of the Alpine Fault and demonstrates that major active and seismogenic structures remain uncharacterized in densely forested regions on Earth.

  7. Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice

    PubMed Central

    Nishimune, Hiroshi; Badawi, Yomna; Mori, Shuuichi; Shigemoto, Kazuhiro

    2016-01-01

    Presynaptic active zones play a pivotal role as synaptic vesicle release sites for synaptic transmission, but the molecular architecture of active zones in mammalian neuromuscular junctions (NMJs) at sub-diffraction limited resolution remains unknown. Bassoon and Piccolo are active zone specific cytosolic proteins essential for active zone assembly in NMJs, ribbon synapses, and brain synapses. These proteins are thought to colocalize and share some functions at active zones. Here, we report an unexpected finding of non-overlapping localization of these two proteins in mouse NMJs revealed using dual-color stimulated emission depletion (STED) super resolution microscopy. Piccolo puncta sandwiched Bassoon puncta and aligned in a Piccolo-Bassoon-Piccolo structure in adult NMJs. P/Q-type voltage-gated calcium channel (VGCC) puncta colocalized with Bassoon puncta. The P/Q-type VGCC and Bassoon protein levels decreased significantly in NMJs from aged mouse. In contrast, the Piccolo levels in NMJs from aged mice were comparable to levels in adult mice. This study revealed the molecular architecture of active zones in mouse NMJs at sub-diffraction limited resolution, and described the selective degeneration mechanism of active zone proteins in NMJs from aged mice. Interestingly, the localization pattern of active zone proteins described herein is similar to active zone structures described using electron microscope tomography. PMID:27321892

  8. Group Dynamics in the Language Classroom: Embodied Participation as Active Reception in the Collective Zone of Proximal Development

    ERIC Educational Resources Information Center

    van Compernolle, Rémi A.; Williams, Lawrence

    2013-01-01

    This article explores the notion of "active reception" during small-group collaborative interaction in the foreign language classroom, focusing on the embodied participation of a secondary (nonspeaking) interactant, Diane. Drawing on Vygotskian sociocultural theory, we argue that within small-group work, a Zone of Proximal Development…

  9. 78 FR 54234 - Foreign-Trade Zone 26-Atlanta, Georgia, Authorization of Production Activity PBR, Inc. d/b/a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-03

    ... Board (15 CFR part 400), including notice in the Federal Register inviting public comment (78 FR 25253... PBR, Inc. d/b/a SKAPS Industries (Polypropylene Geotextiles), Athens, Georgia On April 8, 2013... activity to the Foreign-Trade Zones (FTZ) Board on behalf of PBR, Inc. d/b/a SKAPS Industries...

  10. 78 FR 30269 - Foreign-Trade Zone 129-Bellingham, Washington; Authorization of Production Activity; T.C. Trading...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... Activity; T.C. Trading Company, Inc. (Eyeglass Assembly and Kitting), Blaine, WA On January 17, 2013, the... Foreign-Trade Zones (FTZ) Board on behalf of T.C. Trading Company, Inc., within Subzone 129B, in Blaine... 400), including notice in the Federal Register inviting ] public comment (78 FR 7395, 02/01/2013)....

  11. Abnormal macropore formation during double-sided gas tungsten arc welding of magnesium AZ91D alloy

    SciTech Connect

    Shen Jun You Guoqiang; Long Siyuan; Pan Fusheng

    2008-08-15

    One of the major concerns during gas tungsten arc (GTA) welding of cast magnesium alloys is the presence of large macroporosity in weldments, normally thought to occur from the presence of gas in the castings. In this study, a double-sided GTA welding process was adopted to join wrought magnesium AZ91D alloy plates. Micropores were formed in the weld zone of the first side that was welded, due to precipitation of H{sub 2} as the mushy zone freezes. When the reverse side was welded, the heat generated caused the mushy zone in the initial weld to reform. The micropores in the initial weld then coalesced and expanded to form macropores by means of gas expansion through small holes that are present at the grain boundaries in the partially melted zone. Macropores in the partially melted zone increase with increased heat input, so that when a filler metal is used the macropores are smaller in number and in size.

  12. 77 FR 73415 - Approval for Manufacturing Authority; Foreign-Trade Zone 277; Sub-Zero, Inc. (Refrigerators and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-10

    ... Federal Register (76 FR 70957, 11-16-2011) and the application has been processed pursuant to the FTZ Act.... (Refrigerators and Freezers); Goodyear, AZ Pursuant to its authority under the Foreign-Trade Zones Act of June...

  13. Liparid and macrourid fishes of the hadal zone: in situ observations of activity and feeding behaviour

    PubMed Central

    Jamieson, A.J.; Fujii, T.; Solan, M.; Matsumoto, A.K.; Bagley, P.M.; Priede, I.G.

    2008-01-01

    Using baited camera landers, the first images of living fishes were recorded in the hadal zone (6000–11 000 m) in the Pacific Ocean. The widespread abyssal macrourid Coryphaenoides yaquinae was observed at a new depth record of approximately 7000 m in the Japan Trench. Two endemic species of liparid were observed at similar depths: Pseudoliparis amblystomopsis in the Japan Trench and Notoliparis kermadecensis in the Kermadec Trench. From these observations, we have documented swimming and feeding behaviour of these species and derived the first estimates of hadal fish abundance. The liparids intercepted bait within 100–200 min but were observed to preferentially feed on scavenging amphipods. Notoliparis kermadecensis act as top predators in the hadal food web, exhibiting up to nine suction-feeding events per minute. Both species showed distinctive swimming gaits: P. amblystomopsis (mean length 22.5 cm) displayed a mean tail-beat frequency of 0.47 Hz and mean caudal : pectoral frequency ratio of 0.76, whereas N. kermadecensis (mean length 31.5 cm) displayed respective values of 1.04 and 2.08 Hz. Despite living at extreme depths, these endemic liparids exhibit similar activity levels compared with shallow-water liparids. PMID:19129104

  14. Tetraspanin 7 regulates sealing zone formation and the bone-resorbing activity of osteoclasts.

    PubMed

    Kwon, Jun-Oh; Lee, Yong Deok; Kim, Haemin; Kim, Min Kyung; Song, Min-Kyoung; Lee, Zang Hee; Kim, Hong-Hee

    2016-09-01

    Tetraspanin family proteins regulate morphology, motility, fusion, and signaling in various cell types. We investigated the role of the tetraspanin 7 (Tspan7) isoform in the differentiation and function of osteoclasts. Tspan7 was up-regulated during osteoclastogenesis. When Tspan7 expression was reduced in primary precursor cells by siRNA-mediated gene knock-down, the generation of multinuclear osteoclasts was not affected. However, a striking cytoskeletal abnormality was observed: the formation of the podosome belt structure was inhibited and the microtubular network were disrupted by Tspan7 knock-down. Decreases in acetylated microtubules and levels of phosphorylated Src and Pyk2 in Tspan7 knock-down cells supported the involvement of Tspan7 in cytoskeletal rearrangement signaling in osteoclasts. This cytoskeletal defect interfered with sealing zone formation and subsequently the bone-resorbing activity of mature osteoclasts on dentin surfaces. Our results suggest that Tspan7 plays an important role in cytoskeletal organization required for the bone-resorbing function of osteoclasts by regulating signaling to Src, Pyk2, and microtubules. PMID:27416754

  15. Liparid and macrourid fishes of the hadal zone: in situ observations of activity and feeding behaviour.

    PubMed

    Jamieson, A J; Fujii, T; Solan, M; Matsumoto, A K; Bagley, P M; Priede, I G

    2009-03-22

    Using baited camera landers, the first images of living fishes were recorded in the hadal zone (6000-11000 m) in the Pacific Ocean. The widespread abyssal macrourid Coryphaenoides yaquinae was observed at a new depth record of approximately 7000 m in the Japan Trench. Two endemic species of liparid were observed at similar depths: Pseudoliparis amblystomopsis in the Japan Trench and Notoliparis kermadecensis in the Kermadec Trench. From these observations, we have documented swimming and feeding behaviour of these species and derived the first estimates of hadal fish abundance. The liparids intercepted bait within 100-200 min but were observed to preferentially feed on scavenging amphipods. Notoliparis kermadecensis act as top predators in the hadal food web, exhibiting up to nine suction-feeding events per minute. Both species showed distinctive swimming gaits: P. amblystomopsis (mean length 22.5 cm) displayed a mean tail-beat frequency of 0.47 Hz and mean caudal:pectoral frequency ratio of 0.76, whereas N. kermadecensis (mean length 31.5 cm) displayed respective values of 1.04 and 2.08 Hz. Despite living at extreme depths, these endemic liparids exhibit similar activity levels compared with shallow-water liparids. PMID:19129104

  16. Establishment of Active Traces of Lower Tagus Valley Fault Zone through an Integrated Approach

    NASA Astrophysics Data System (ADS)

    Besana-Ostman, G. M.; Vilanova, S.; Flor, A.; Canora, C.; Heleno, S.; Domingues, A.; Narciso, J.; Pinheiro, P.; Pinto, L.; Fonseca, J. F.

    2013-05-01

    Despite the occurrence of at least two damaging earthquakes in historical times - the M~7 1531 and the M6 1909 earthquakes - the Lower Tagus Valley Fault Zone (LTVFZ) has only recently been mapped (Besana-Ostman et al., 2012). In addition, a new set of active traces has been identified to the east during recent analysis and field inspections. The major challenges to the identification of active traces within Lower Tagus Valley (LTV) are both the presence of the very dynamic Tagus River (LTR) and the extensive urban and agricultural modifications introduced in the landscape. The detailed reports on the geological effects of the 1909 earthquake, while documenting extensively the secondary, shaking-related effects, provide no indication of surface rupture. The active traces of the northeast-southwest trending left-lateral LTVFZ within the LTV were established through integrated approaches as follows: aerial photo analysis, drainage system and satellite images examination, geomorphic feature identification, field mapping, geomorphic index measurements and trenching. The mapped traces extend to about 80 kilometers long and transect Quaternary and Holocene deposits. The mapped length of the western splay is compatible with an M7.2 earthquake. On the other hand, the newly mapped eastern traces plot almost parallel with the western splay, which may extend southwards to a comparable length. Preliminary analysis of satellite data show some evidence of additional splays located further east and south relative to the LTV. The new active traces suggest that the LTVFZ is a left-stepping left-lateral fault system with a regional NNE-SSW trend. Moreover, its extent and kinematics suggest magnitudes higher than previously assessed for the region. The location of the active traces displays a better correlation with the damage distribution of the historical events. Given the significance and implications of these findings for earthquake hazards assessment in Portugal, further studies

  17. Microstructure and properties of welds between 5754 Al alloys and AZ31 Mg alloys using a Yb:YAG laser

    NASA Astrophysics Data System (ADS)

    Bannour, Sana; Autric, Michel; Masse, Jean-Eric; Mattei, Simone; Mhiri, Hatem

    2015-02-01

    Dissimilar laser beam welding between A5754 Al alloys and AZ31 Mg alloys with the plate thickness of 2 mm was investigated. Complex flow pattern characterized by a large volume of intermetallic compounds Al12Mg17 and Al3Mg2 is formed in the fusion zone. Microhardness measurement of the dissimilar welds presents an uneven distribution due to the complicated microstructure of the weld, and the maximum value of microhardness in the fusion zone is much higher than of the base materials.

  18. Rates of volcanic activity along the southwest rift zone of Mauna Loa volcano, Hawaii.

    USGS Publications Warehouse

    Lipman, P.W.

    1981-01-01

    Flow-by-flow mapping of the 65 km long subaerial part of the southwest rift zone and adjacent flanks of Mauna Loa Volcano, Hawaii, and about 50 new 14C dates on charcoal from beneath these flows permit estimates of rates of lava accumulation and volcanic growth over the past 10 000 years. The sequence of historic eruptions along the southwest rift zone, beginning in 1868, shows a general pattern of uprift migration and increasing eruptive volume, culminating in the great 1950 eruption. No event comparable to 1950, in terms of volume or vent length, is evident for at least the previous 1000 years. Rates of lava accumulation along the zone have been subequal to those of Kilauea Volcano during the historic period but they were much lower in late prehistoric time (unpubl. Kilauea data by R. T. Holcomb). Rates of surface covering and volcanic growth have been markedly asymmetric along Mauna Loa's southwest rift zone. Accumulation rates have been about half again as great on the northwest side of the rift zone in comparison with the southeast side. The difference apparently reflects a westward lateral shift of the rift zone of Mauna Loa away from Kilauea Volcano, which may have acted as a barrier to symmetrical growth of the rift zone. -Author

  19. AZ 242, a novel PPARalpha/gamma agonist with beneficial effects on insulin resistance and carbohydrate and lipid metabolism in ob/ob mice and obese Zucker rats.

    PubMed

    Ljung, Bengt; Bamberg, Krister; Dahllöf, Björn; Kjellstedt, Ann; Oakes, Nicholas D; Ostling, Jörgen; Svensson, Lennart; Camejo, Germán

    2002-11-01

    Abnormalities in fatty acid (FA) metabolism underlie the development of insulin resistance and alterations in glucose metabolism, features characteristic of the metabolic syndrome and type 2 diabetes that can result in an increased risk of cardiovascular disease. We present pharmacodynamic effects of AZ 242, a novel peroxisome proliferator activated receptor (PPAR)alpha/gamma agonist. AZ 242 dose-dependently reduced the hypertriglyceridemia, hyperinsulinemia, and hyperglycemia of ob/ob diabetic mice. Euglycemic hyperinsulinemic clamp studies showed that treatment with AZ 242 (1 micromol/kg/d) restored insulin sensitivity of obese Zucker rats and decreased insulin secretion. In vitro, in reporter gene assays, AZ 242 activated human PPARalpha and PPARgamma with EC(50) in the micro molar range. It also induced differentiation in 3T3-L1 cells, an established PPARgamma effect, and caused up-regulation of liver fatty acid binding protein in HepG-2 cells, a PPARalpha-mediated effect. PPARalpha-mediated effects of AZ 242 in vivo were documented by induction of hepatic cytochrome P 450-4A in mice. The results indicate that the dual PPARalpha/gamma agonism of AZ 242 reduces insulin resistance and has beneficial effects on FA and glucose metabolism. This effect profile could provide a suitable therapeutic approach to the treatment of type 2 diabetes, metabolic syndrome, and associated vascular risk factors. PMID:12401884

  20. Living microbial ecosystems within the active zone of catagenesis: Implications for feeding the deep biosphere

    NASA Astrophysics Data System (ADS)

    Horsfield, B.; Schenk, H. J.; Zink, K.; Ondrak, R.; Dieckmann, V.; Kallmeyer, J.; Mangelsdorf, K.; di Primio, R.; Wilkes, H.; Parkes, R. J.; Fry, J.; Cragg, B.

    2006-06-01

    Earth's largest reactive carbon pool, marine sedimentary organic matter, becomes increasingly recalcitrant during burial, making it almost inaccessible as a substrate for microorganisms, and thereby limiting metabolic activity in the deep biosphere. Because elevated temperature acting over geological time leads to the massive thermal breakdown of the organic matter into volatiles, including petroleum, the question arises whether microorganisms can directly utilize these maturation products as a substrate. While migrated thermogenic fluids are known to sustain microbial consortia in shallow sediments, an in situ coupling of abiotic generation and microbial utilization has not been demonstrated. Here we show, using a combination of basin modelling, kinetic modelling, geomicrobiology and biogeochemistry, that microorganisms inhabit the active generation zone in the Nankai Trough, offshore Japan. Three sites from ODP Leg 190 have been evaluated, namely 1173, 1174 and 1177, drilled in nearly undeformed Quaternary and Tertiary sedimentary sequences seaward of the Nankai Trough itself. Paleotemperatures were reconstructed based on subsidence profiles, compaction modelling, present-day heat flow, downhole temperature measurements and organic maturity parameters. Today's heat flow distribution can be considered mainly conductive, and is extremely high in places, reaching 180 mW/m 2. The kinetic parameters describing total hydrocarbon generation, determined by laboratory pyrolysis experiments, were utilized by the model in order to predict the timing of generation in time and space. The model predicts that the onset of present day generation lies between 300 and 500 m below sea floor (5100-5300 m below mean sea level), depending on well location. In the case of Site 1174, 5-10% conversion has taken place by a present day temperature of ca. 85 °C. Predictions were largely validated by on-site hydrocarbon gas measurements. Viable organisms in the same depth range have been

  1. Effects of heat input on microstructure and tensile properties of laser welded magnesium alloy AZ31

    SciTech Connect

    Quan, Y.J. Chen, Z.H.; Gong, X.S.; Yu, Z.H.

    2008-10-15

    A 3 kW CO{sub 2} laser beam was used to join wrought magnesium alloy AZ31 sheets, and the effects of heat input on the quality of butt welding joints were studied. By macro and microanalysis, it is found that the welding heat input plays an important role in laser welding process for AZ31 wrought sheets. After welding, the grains far from the weld centre present the typical rolled structure. But the microstructure out of the fusion zone gradually changes to complete equiaxed crystals as the distance from the weld centre decreases. Adjacent to the fusion boundary, there is a band region with columnar grains, and its growth direction is obviously perpendicular to the solid/liquid line. The microstructure in fusion centre consists of fine equiaxed grains and the many precipitated particles are brittle phase Mg{sub 17}Al{sub 12} or Mg{sub 17}(Al,Zn){sub 12}. With increasing the heat input, the band width of columnar grains varies, the grains in fusion zone get coarser, and the distribution of precipitates changes from intragranularly scattered particles to intergranularly packed ones. The results of tensile test show that the change trend of ultimate tensile strength (UTS) and elongation of the welded joints is to increase at first and then decrease with the heat input increasing. When the heat input reaches 24 J mm{sup -1}, the maximum value of the UTS is up to 96.8% of the base metal.

  2. Characterization of AZ31B wrought magnesium alloy joints welded by high power fiber laser

    SciTech Connect

    Wang Zemin; Gao Ming Tang Haiguo; Zeng Xiaoyan

    2011-10-15

    A 6 kW fiber laser is used to weld AZ31B wrought magnesium alloy and the characterization of welded joints are studied by the observations of bead size, microstructure and mechanical properties. The accepted joints without macro-defects can be obtained when the laser power is in the range of 2.5 to 4.0 kW. Typical hexagonal dendrites are observed in the fusion zone, whose average semi-axis length increases with increasing heat input or decreasing welding speed. The minimum ultimate tensile strength of welded joints reaches 227 MPa, 94.6% of the base metal. And when the heat input reduces to 48 J/mm or lower, the joints are fractured in the base metal, showing stronger failure strength compared to the base metal. For the joints ruptured in the weld metal, the fracture surface is characterized by a ductile-brittle mixed pattern consisting of both dimples and cleavages. Finally, the formation mechanism of pore in the welds is discussed and summarized by the pore morphologies on the fracture surface. - Highlights: {yields} Accepted joints of AZ31B Mg alloy are produced by high power fiber laser. {yields} Optimal welding parameters are summarized by experimental observations. {yields} Obvious hexagonal dendrites are observed in the fusion zone. {yields} The joints are stronger than base metal as the heat input is lower than 48 J/mm. {yields} Pore formation mechanism of welded joints is discussed and summarized.

  3. Evolution of Surface Motor Activation Zones in Hemiplegic Patients During 20 Sessions of FES Therapy with Multi-pad Electrodes.

    PubMed

    Malešević, Jovana; Štrbac, Matija; Isaković, Milica; Kojić, Vladimir; Konstantinović, Ljubica; Vidaković, Aleksandra; Dedijer, Suzana; Kostić, Miloš; Keller, Thierry

    2016-06-13

    The purpose of this study was to examine surface motor activation zones for wrist, fingers and thumb extension movements and their temporal change during 20 therapy sessions using advanced multi-pad functional electrical stimulation system. Results from four hemiplegic patients indicate that certain zones have higher probability of eliciting each of the target movements. However, mutual overlap and variations of the zones are present not just between the subjects, but also on the intrasubject level, reflected through these session to session transformations of the selected virtual electrodes. The obtained results could be used as a priori knowledge for semi-automated optimization algorithm and could shorten the time required for calibration of the multi-pad electrode. PMID:27478575

  4. Evolution of Surface Motor Activation Zones in Hemiplegic Patients During 20 Sessions of FES Therapy with Multi-pad Electrodes

    PubMed Central

    Malešević, Jovana; Štrbac, Matija; Isaković, Milica; Kojić, Vladimir; Konstantinović, Ljubica; Vidaković, Aleksandra; Dedijer, Suzana; Kostić, Miloš; Keller, Thierry

    2016-01-01

    The purpose of this study was to examine surface motor activation zones for wrist, fingers and thumb extension movements and their temporal change during 20 therapy sessions using advanced multi-pad functional electrical stimulation system. Results from four hemiplegic patients indicate that certain zones have higher probability of eliciting each of the target movements. However, mutual overlap and variations of the zones are present not just between the subjects, but also on the intrasubject level, reflected through these session to session transformations of the selected virtual electrodes. The obtained results could be used as a priori knowledge for semi-automated optimization algorithm and could shorten the time required for calibration of the multi-pad electrode. PMID:27478575

  5. CESIUM REMOVAL FROM TANKS 241-AN-103 & 241-SX-105 & 241-AZ-101 & 241AZ-102 COMPOSITE FOR TESTING IN BENCH SCALE STEAM REFORMER

    SciTech Connect

    DUNCAN JB; HUBER HJ

    2011-04-21

    This report documents the preparation of three actual Hanford tank waste samples for shipment to the Savannah River National Laboratory (SRNL). Two of the samples were dissolved saltcakes from tank 241-AN-103 (hereafter AN-103) and tank 241-SX-105 (hereafter SX-105); one sample was a supernate composite from tanks 241-AZ-101 and 241-AZ-102 (hereafter AZ-101/102). The preparation of the samples was executed following the test plans LAB-PLAN-10-00006, Test Plan for the Preparation of Samples from Hanford Tanks 241-SX-105, 241-AN-103, 241-AN-107, and LAB-PLN-l0-00014, Test Plan for the Preparation of a Composite Sample from Hanford Tanks 241-AZ-101 and 241-AZ-102 for Steam Reformer Testing at the Savannah River National Laboratory. All procedural steps were recorded in laboratory notebook HNF-N-274 3. Sample breakdown diagrams for AN-103 and SX-105 are presented in Appendix A. The tank samples were prepared in support of a series of treatability studies of the Fluidized Bed Steam Reforming (FBSR) process using a Bench-Scale Reformer (BSR) at SRNL. Tests with simulants have shown that the FBSR mineralized waste form is comparable to low-activity waste glass with respect to environmental durability (WSRC-STI-2008-00268, Mineralization of Radioactive Wastes by Fluidized Bed Steam Reforming (FBSR): Comparisons to Vitreous Waste Forms and Pertinent Durability Testing). However, a rigorous assessment requires long-term performance data from FBSR product formed from actual Hanford tank waste. Washington River Protection Solutions, LLC (WRPS) has initiated a Waste Form Qualification Program (WP-5.2.1-2010-001, Fluidized Bed Steam Reformer Low-level Waste Form Qualification) to gather the data required to demonstrate that an adequate FBSR mineralized waste form can be produced. The documentation of the selection process of the three tank samples has been separately reported in RPP-48824, Sample Selection Process for Bench-Scale Steam Reforming Treatability Studies Using

  6. ARSENIC REMOVAL FROM DRINKING WATER BY ADSORPTIVE MEDIA. USEPA DEMONSTRATION PROJECT AT VALLEY VISTA, AZ FINAL PERFORMANCE EVALUATION REPORT

    EPA Science Inventory

    This report documents the activities performed during and the results obtained from the arsenic removal treatment technology demonstration project at an Arizona Water Company (AWC) facility in Sedona, AZ, commonly referred to as Valley Vista. The objectives of the project were t...

  7. ARSENIC REMOVAL FROM DRINKING WATER BY ADSORPTIVE MEDIA USEPA DEMONSTRATION PROJECT AT VALLEY VISTA, AZ SIX-MONTH EVALUATION REPORT

    EPA Science Inventory

    This report documents the activities performed and the results obtained from the first six months of the EPA arsenic removal technology demonstration project at the Arizona Water Company (AWC) facility in Sedona, AZ, commonly referred to as Valley Vista. The main objective of the...

  8. ARSENIC REMOVAL FROM DRINKING WATER BY ADSORPTIVE MEDIA, USEPA DEMONSTRATION PROJECT AT RIMROCK, AZ, SIX-MONTH EVALUATION REPORT

    EPA Science Inventory

    This report documents the activities performed during and the results obtained from the first six months of the arsenic removal treatment technology demonstration project at Rimrock, AZ. The objectives of the project are to evaluate the effectiveness of AdEdge Arsenic Package Uni...

  9. Arsenic Removal from Drinking Water by Adsorptive Media USEPA Demonstration Project at Rimrock AZ Final Performance Evaluation Report

    EPA Science Inventory

    This report documents the activities performed during and the results obtained from the arsenic removal treatment technology demonstration project at the Arizona Water Company (AWC) facility in Rimrock, AZ. The objectives of the project were to evaluate: 1) the effectiveness of ...

  10. Project W-314 specific test and evaluation plan for AZ tank farm upgrades

    SciTech Connect

    Hays, W.H.

    1998-08-12

    The purpose of this Specific Test and Evaluation Plan (STEP) is to provide a detailed written plan for the systematic testing of modifications made by the addition of the SN-631 transfer line from the AZ-O1A pit to the AZ-02A pit by the W-314 Project. The STEP develops the outline for test procedures that verify the system`s performance to the established Project design criteria. The STEP is a lower tier document based on the W-314 Test and Evaluation P1 an (TEP). Testing includes Validations and Verifications (e.g., Commercial Grade Item Dedication activities, etc), Factory Tests and Inspections (FTIs), installation tests and inspections, Construction Tests and Inspections (CTIs), Acceptance Test Procedures (ATPs), Pre-Operational Test Procedures (POTPs), and Operational Test Procedures (OTPs). The STEP will be utilized in conjunction with the TEP for verification and validation.

  11. Effect of cw-CO2 laser surface treatment on structure and properties of AZ91 magnesium alloy

    NASA Astrophysics Data System (ADS)

    Iwaszko, Józef; Strzelecka, Monika

    2016-06-01

    In the study, samples of AZ91 magnesium alloy were subjected to a surface remelting treatment by means of a continuous wave (cw) CO2 laser. The scope of the investigation included both macro- and microstructural examination, hardness measurements, and wear resistance tests. The investigation has shown that remelting treatment leads to a strong refinement of structure in the surface layer and a more even distribution of phases. Fine α-phase dendrites have been observed to dominate in the remelting zone. The dendritic arm spacing in the laser treated surface was in the range of 1-2.5 μm. The structural changes triggered by remelting have contributed to an increase in the hardness and the wear resistance of AZ91 alloy. The microhardness of the remelted zone has increased to 71-93 HV0.05 for single-strip remelting and to 84-107 HV0.05 for multi-strip remelting in comparison with about ~60 HV0.05 for untreated alloy. The friction coefficient has decreased from 0.375 for material w/o treatment to 0.311 for remelted material. SEM investigations of samples after tribological tests have revealed the presence of parallel grooves proving the occurrence of microploughing and micro cutting of the material during the tribological testing. The results of the conducted investigation have indicated a beneficial influence of the cw-CO2 laser remelting treatment on the structure and properties of AZ91 alloy.

  12. Shoreline changes and its impact on activities in the coastal zone in Greenland

    NASA Astrophysics Data System (ADS)

    Kroon, A.; Bendixen, M.; Elberling, B.

    2015-12-01

    shorelines. The shoreline changes were estimated using the digital shoreline analysis system (DSAS) of the USGS. The spatial variability of accumulation and erosion patterns was detected and shows a surprising thread for ancient settlements and present-day activities in the coastal zone. The same patterns are finally discussed in terms of coastal risk assessment.

  13. Exoplanet detection. Stellar activity masquerading as planets in the habitable zone of the M dwarf Gliese 581.

    PubMed

    Robertson, Paul; Mahadevan, Suvrath; Endl, Michael; Roy, Arpita

    2014-07-25

    The M dwarf star Gliese 581 is believed to host four planets, including one (GJ 581d) near the habitable zone that could possibly support liquid water on its surface if it is a rocky planet. The detection of another habitable-zone planet--GJ 581g--is disputed, as its significance depends on the eccentricity assumed for d. Analyzing stellar activity using the Hα line, we measure a stellar rotation period of 130 ± 2 days and a correlation for Hα modulation with radial velocity. Correcting for activity greatly diminishes the signal of GJ 581d (to 1.5 standard deviations) while significantly boosting the signals of the other known super-Earth planets. GJ 581d does not exist, but is an artifact of stellar activity which, when incompletely corrected, causes the false detection of planet g. PMID:24993348

  14. Present-day submarine hydrothermal activity in the Taupo-Rotorua Zone (Bay of Plenty, New Zealand)

    SciTech Connect

    Osipenko, A.B.; Egorov, Yu.O.; Fazlullin, S.M.; Gavrilenko, G.M.; Shul`kin, V.I.; Chertkova, L.V.

    1994-09-01

    We made detailed descriptions of the structure and material composition of sedimentary and water columns in the vicinity of active submarine hydrothermal activity in the southern part of the Bay of Plenty (North Island, New Zealand). Geophysical methods revealed that the hydrothermal system is confined to a tectonically distinct zone with a sedimentary cover characterized by complex structure. Chemical and mineralogical investigations confirmed that the activity of underwater vents exerts no substantial regional influence on the composition and features of ore mineralization in these formations. It is shown that essentially hydrothermal formations distinguishable within areas of otherwise monotypic sediments directly coincide with zones of hydrothermal discharge in the ocean floor. The absence of pronounced hydrothermal anomalies, together with the presence of {open_quotes}tongues{close_quotes} of anomalous concentrations of water-soluble gases suggests that the discharges are primarily hydrothermal in character.

  15. 75 FR 81190 - Television Broadcasting Services; Yuma, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-27

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Television Broadcasting Services; Yuma, AZ AGENCY: Federal Communications Commission. ACTION: Dismissal. SUMMARY: The Commission dismisses the petition for rulemaking filed by...

  16. Simultaneous Segmentation of Prostatic Zones Using Active Appearance Models With Multiple Coupled Levelsets

    PubMed Central

    Toth, Robert; Ribault, Justin; Gentile, John; Sperling, Dan; Madabhushi, Anant

    2013-01-01

    In this work we present an improvement to the popular Active Appearance Model (AAM) algorithm, that we call the Multiple-Levelset AAM (MLA). The MLA can simultaneously segment multiple objects, and makes use of multiple levelsets, rather than anatomical landmarks, to define the shapes. AAMs traditionally define the shape of each object using a set of anatomical landmarks. However, landmarks can be difficult to identify, and AAMs traditionally only allow for segmentation of a single object of interest. The MLA, which is a landmark independent AAM, allows for levelsets of multiple objects to be determined and allows for them to be coupled with image intensities. This gives the MLA the flexibility to simulataneously segmentation multiple objects of interest in a new image. In this work we apply the MLA to segment the prostate capsule, the prostate peripheral zone (PZ), and the prostate central gland (CG), from a set of 40 endorectal, T2-weighted MRI images. The MLA system we employ in this work leverages a hierarchical segmentation framework, so constructed as to exploit domain specific attributes, by utilizing a given prostate segmentation to help drive the segmentations of the CG and PZ, which are embedded within the prostate. Our coupled MLA scheme yielded mean Dice accuracy values of .81, .79 and .68 for the prostate, CG, and PZ, respectively using a leave-one-out cross validation scheme over 40 patient studies. When only considering the midgland of the prostate, the mean DSC values were .89, .84, and .76 for the prostate, CG, and PZ respectively. PMID:23997571

  17. Simultaneous Segmentation of Prostatic Zones Using Active Appearance Models With Multiple Coupled Levelsets.

    PubMed

    Toth, Robert; Ribault, Justin; Gentile, John; Sperling, Dan; Madabhushi, Anant

    2013-09-01

    In this work we present an improvement to the popular Active Appearance Model (AAM) algorithm, that we call the Multiple-Levelset AAM (MLA). The MLA can simultaneously segment multiple objects, and makes use of multiple levelsets, rather than anatomical landmarks, to define the shapes. AAMs traditionally define the shape of each object using a set of anatomical landmarks. However, landmarks can be difficult to identify, and AAMs traditionally only allow for segmentation of a single object of interest. The MLA, which is a landmark independent AAM, allows for levelsets of multiple objects to be determined and allows for them to be coupled with image intensities. This gives the MLA the flexibility to simulataneously segmentation multiple objects of interest in a new image. In this work we apply the MLA to segment the prostate capsule, the prostate peripheral zone (PZ), and the prostate central gland (CG), from a set of 40 endorectal, T2-weighted MRI images. The MLA system we employ in this work leverages a hierarchical segmentation framework, so constructed as to exploit domain specific attributes, by utilizing a given prostate segmentation to help drive the segmentations of the CG and PZ, which are embedded within the prostate. Our coupled MLA scheme yielded mean Dice accuracy values of .81, .79 and .68 for the prostate, CG, and PZ, respectively using a leave-one-out cross validation scheme over 40 patient studies. When only considering the midgland of the prostate, the mean DSC values were .89, .84, and .76 for the prostate, CG, and PZ respectively. PMID:23997571

  18. Microbial abundance and activities in relation to water potential in the vadose zones of arid and semiarid sites.

    PubMed

    Kieft, T L; Amy, P S; Brockman, F J; Fredrickson, J K; Bjornstad, B N; Rosacker, L L

    1993-07-01

    Numbers and activities of microorganisms were measured in the vadose zones of three arid and semiarid areas of the western United States, and the influence of water availability was determined. These low-moisture environments have vadose zones that are commonly hundreds of meters thick. The specific sampling locations chosen were on or near U.S. Department of Energy facilities: the Nevada Test Site (NTS), the Idaho National Engineering Laboratory (INEL), and the Hanford Site (HS) in southcentral Washington State. Most of the sampling locations were uncontaminated, but geologically representative of nearby locations with storage and/or leakage of waste compounds in the vadose zone. Lithologies of samples included volcanic tuff, basalt, glaciofluvial and fluvial sediments, and paleosols (buried soils). Samples were collected aseptically, either by drilling bore-holes (INEL and HS), or by excavation within tunnels (NTS) and outcrop faces (paleosols near the HS). Total numbers of microorganisms were counted using direct microscopy, and numbers of culturable microorganisms were determined using plate-count methods. Desiccation-tolerant microorganisms were quantified by plate counts performed after 24 h desiccation of the samples. Mineralization of (14)C-labeled glucose and acetate was quantified in samples at their ambient moisture contents, in dried samples, and in moistened samples, to test the hypothesis that water limits microbial activities in vadose zones. Total numbers of microorganisms ranged from log 4.5 to 7.1 cells g(-1) dry wt. Culturable counts ranged from log <2 to 6.7 CFU g(-1) dry wt, with the highest densities occurring in paleosol (buried soil) samples. Culturable cells appeared to be desiccation-tolerant in nearly all samples that had detectable viable heterotrophs. Water limited mineralization in some, but not all samples, suggesting that an inorganic nutrient or other factor may limit microbial activities in some vadose zone environments. PMID

  19. Study on the microstructural evolution of AZ31 magnesium alloy in a vertical twin-roll casting process

    NASA Astrophysics Data System (ADS)

    Chen, Ming; Hu, Xiao-Dong; Han, Bing; Deng, Xiao-Hu; Ju, Dong-Ying

    2016-02-01

    Finite element method was employed to calculate the macroflow velocity and temperature distribution of the pool domain's biting zone in twin-roll casting. Macroanalysis results were inducted as boundary conditions into microanalysis. Phase field method (PFM) was adopted to investigate the microstructure evolution. Based on the Kim-Kim-Suzuki model, the effect of metal flow velocity was coupled on the solute gradient item, and the real physical parameters of AZ31 were inducted into the numerical calculation. We used the marker and cell method in the discrete element solution of microstructural pattern prediction of AZ31 magnesium alloys. The different flow velocity values that predicted the columnar dendrite evolution were discussed in detail. Numerical simulation results were also compared with the experiment analysis. The microstructure obtained by PFM agrees with the actual pattern observed via optical microscopy.

  20. Pea Broth Enhances the Biocontrol Efficacy of Lysobacter capsici AZ78 by Triggering Cell Motility Associated with Biogenesis of Type IV Pilus.

    PubMed

    Tomada, Selena; Puopolo, Gerardo; Perazzolli, Michele; Musetti, Rita; Loi, Nazia; Pertot, Ilaria

    2016-01-01

    Bacterial cells can display different types of motility, due to the presence of external appendages such as flagella and type IV pili. To date, little information on the mechanisms involved in the motility of the Lysobacter species has been available. Recently, L. capsici AZ78, a biocontrol agent of phytopathogenic oomycetes, showed the ability to move on jellified pea broth. Pea broth medium improved also the biocontrol activity of L. capsici AZ78 against Plasmopara viticola under greenhouse conditions. Noteworthy, the quantity of pea residues remaining on grapevine leaves fostered cell motility in L. capsici AZ78. Based on these results, this unusual motility related to the composition of the growth medium was investigated in bacterial strains belonging to several Lysobacter species. The six L. capsici strains tested developed dendrite-like colonies when grown on jellified pea broth, while the development of dendrite-like colonies was not recorded in the media commonly used in motility assays. To determine the presence of genes responsible for biogenesis of the flagellum and type IV pili, the genome of L. capsici AZ78 was mined. Genes encoding structural components and regulatory factors of type IV pili were upregulated in L. capsici AZ78 cells grown on the above-mentioned medium, as compared with the other tested media. These results provide new insight into the motility mechanism of L. capsici members and the role of type IV pili and pea compounds on the epiphytic fitness and biocontrol features of L. capsici AZ78. PMID:27507963

  1. Pea Broth Enhances the Biocontrol Efficacy of Lysobacter capsici AZ78 by Triggering Cell Motility Associated with Biogenesis of Type IV Pilus

    PubMed Central

    Tomada, Selena; Puopolo, Gerardo; Perazzolli, Michele; Musetti, Rita; Loi, Nazia; Pertot, Ilaria

    2016-01-01

    Bacterial cells can display different types of motility, due to the presence of external appendages such as flagella and type IV pili. To date, little information on the mechanisms involved in the motility of the Lysobacter species has been available. Recently, L. capsici AZ78, a biocontrol agent of phytopathogenic oomycetes, showed the ability to move on jellified pea broth. Pea broth medium improved also the biocontrol activity of L. capsici AZ78 against Plasmopara viticola under greenhouse conditions. Noteworthy, the quantity of pea residues remaining on grapevine leaves fostered cell motility in L. capsici AZ78. Based on these results, this unusual motility related to the composition of the growth medium was investigated in bacterial strains belonging to several Lysobacter species. The six L. capsici strains tested developed dendrite-like colonies when grown on jellified pea broth, while the development of dendrite-like colonies was not recorded in the media commonly used in motility assays. To determine the presence of genes responsible for biogenesis of the flagellum and type IV pili, the genome of L. capsici AZ78 was mined. Genes encoding structural components and regulatory factors of type IV pili were upregulated in L. capsici AZ78 cells grown on the above-mentioned medium, as compared with the other tested media. These results provide new insight into the motility mechanism of L. capsici members and the role of type IV pili and pea compounds on the epiphytic fitness and biocontrol features of L. capsici AZ78. PMID:27507963

  2. Aftershocks illuninate the 2011 Mineral, Virginia, earthquake causative fault zone and nearby active faults

    USGS Publications Warehouse

    Horton, Jr., J. Wright; Shah, Anjana K.; McNamara, Daniel E.; Snyder, Stephen L.; Carter, Aina M

    2015-01-01

    Deployment of temporary seismic stations after the 2011 Mineral, Virginia (USA), earthquake produced a well-recorded aftershock sequence. The majority of aftershocks are in a tabular cluster that delineates the previously unknown Quail fault zone. Quail fault zone aftershocks range from ~3 to 8 km in depth and are in a 1-km-thick zone striking ~036° and dipping ~50°SE, consistent with a 028°, 50°SE main-shock nodal plane having mostly reverse slip. This cluster extends ~10 km along strike. The Quail fault zone projects to the surface in gneiss of the Ordovician Chopawamsic Formation just southeast of the Ordovician–Silurian Ellisville Granodiorite pluton tail. The following three clusters of shallow (<3 km) aftershocks illuminate other faults. (1) An elongate cluster of early aftershocks, ~10 km east of the Quail fault zone, extends 8 km from Fredericks Hall, strikes ~035°–039°, and appears to be roughly vertical. The Fredericks Hall fault may be a strand or splay of the older Lakeside fault zone, which to the south spans a width of several kilometers. (2) A cluster of later aftershocks ~3 km northeast of Cuckoo delineates a fault near the eastern contact of the Ordovician Quantico Formation. (3) An elongate cluster of late aftershocks ~1 km northwest of the Quail fault zone aftershock cluster delineates the northwest fault (described herein), which is temporally distinct, dips more steeply, and has a more northeastward strike. Some aftershock-illuminated faults coincide with preexisting units or structures evident from radiometric anomalies, suggesting tectonic inheritance or reactivation.

  3. Reactive oxygen species regulate leaf pulvinus abscission zone cell separation in response to water-deficit stress in cassava

    PubMed Central

    Liao, Wenbin; Wang, Gan; Li, Yayun; Wang, Bin; Zhang, Peng; Peng, Ming

    2016-01-01

    Cassava (Manihot esculenta Crantz) plant resists water-deficit stress by shedding leaves leading to adaptive water-deficit condition. Transcriptomic, physiological, cellular, molecular, metabolic, and transgenic methods were used to study the mechanism of cassava abscission zone (AZ) cell separation under water-deficit stress. Microscopic observation indicated that AZ cell separation initiated at the later stages during water-deficit stress. Transcriptome profiling of AZ suggested that differential expression genes of AZ under stress mainly participate in reactive oxygen species (ROS) pathway. The key genes involved in hydrogen peroxide biosynthesis and metabolism showed significantly higher expression levels in AZ than non-separating tissues adjacent to the AZ under stress. Significantly higher levels of hydrogen peroxide correlated with hydrogen peroxide biosynthesis related genes and AZ cell separation was detected by microscopic observation, colorimetric detection and GC-MS analyses under stress. Co-overexpression of the ROS-scavenging proteins SOD and CAT1 in cassava decreased the levels of hydrogen peroxide in AZ under water-deficit stress. The cell separation of the pulvinus AZ also delayed in co-overexpression of the ROS-scavenging proteins SOD and CAT1 plants both in vitro and at the plant level. Together, the results indicated that ROS play an important regulatory role in the process of cassava leaf abscission under water-deficit stress. PMID:26899473

  4. Reactive oxygen species regulate leaf pulvinus abscission zone cell separation in response to water-deficit stress in cassava.

    PubMed

    Liao, Wenbin; Wang, Gan; Li, Yayun; Wang, Bin; Zhang, Peng; Peng, Ming

    2016-01-01

    Cassava (Manihot esculenta Crantz) plant resists water-deficit stress by shedding leaves leading to adaptive water-deficit condition. Transcriptomic, physiological, cellular, molecular, metabolic, and transgenic methods were used to study the mechanism of cassava abscission zone (AZ) cell separation under water-deficit stress. Microscopic observation indicated that AZ cell separation initiated at the later stages during water-deficit stress. Transcriptome profiling of AZ suggested that differential expression genes of AZ under stress mainly participate in reactive oxygen species (ROS) pathway. The key genes involved in hydrogen peroxide biosynthesis and metabolism showed significantly higher expression levels in AZ than non-separating tissues adjacent to the AZ under stress. Significantly higher levels of hydrogen peroxide correlated with hydrogen peroxide biosynthesis related genes and AZ cell separation was detected by microscopic observation, colorimetric detection and GC-MS analyses under stress. Co-overexpression of the ROS-scavenging proteins SOD and CAT1 in cassava decreased the levels of hydrogen peroxide in AZ under water-deficit stress. The cell separation of the pulvinus AZ also delayed in co-overexpression of the ROS-scavenging proteins SOD and CAT1 plants both in vitro and at the plant level. Together, the results indicated that ROS play an important regulatory role in the process of cassava leaf abscission under water-deficit stress. PMID:26899473

  5. Growth and interaction of active faults within a nascent shear zone, central Mojave Desert, California

    NASA Astrophysics Data System (ADS)

    Oskin, M.; Strane, M.

    2006-12-01

    Compilation of new slip-distribution and slip-rate data from the Mojave Desert portion of the Eastern California shear zone (ECSZ) lends insight into the role of fault growth and interaction of conjugate fault systems in accommodating shear. Dextral faults of the Mojave Desert ECSZ approach but do not appear to cut the bounding ENE-striking sinistral Pinto Mountain and Garlock faults. Differing styles of accommodation of these bounding faults occur at opposite ends of the 140 km-long NW-striking Hidalgo-Calico-Blackwater dextral fault system. Total slip and slip rate of the Blackwater fault gradually diminish northward. The fault terminates as a single strand with a zero-slip fault tip before intersecting the Garlock fault. In contrast, the Calico and Hidalgo faults spread displacement southward onto multiple fault strands spaced several kilometers apart. Active folding further distributes displacement onto the adjacent Bullion and Mesquite Lake faults. These mechanisms appear to maintain a uniform gradient of displacement approaching the Pinto Mountain fault. The highest displacement (9.8 ± 0.2 km) and slip rate (1.8 ± 0.3 mm/yr) occur in the central part of the Hidalgo-Calico-Blackwater fault system where strain is concentrated onto a single fault strand. A significant drop in total displacement and slip rate occurs along the northern Calico fault. Strain appears to be transferred here onto ENE-striking sinistral faults that separate domains of clockwise rotation in the central Mojave Desert. The kinematically incompatible intersection of sinistral and dextral faults is accommodated, at least in part, by active folding and uplift of the Calico Mountains and Mud Hills. Total slip and slip rate are not correlative for dextral faults of the Mojave ECSZ, indicating ongoing evolution of the fault network. For example, the Lenwood fault is a highly segmented, immature dextral fault with only 1.0 ± 0.1 km of total displacement yet its slip rate (1.5 ± 0.4 mm/yr) is

  6. More Active Living–oriented County and Municipal Zoning is Associated with Increased Adult Leisure Time Physical Activity—United States, 2011

    PubMed Central

    Chriqui, Jamie F.; Nicholson, Lisa M.; Thrun, Emily; Leider, Julien; Slater, Sandy J.

    2016-01-01

    Although zoning is recognized for its role in facilitating healthy communities, no study has examined whether active living-oriented zoning codes are associated with adult leisure time physical activity (PA). This study sought to fill this gap and hypothesized that adult leisure time PA would be greater in communities with more progressive zoning code reforms and more active living-oriented zoning. Zoning codes for 1,617 county and municipal jurisdictions located in 30 states (covering ~40% of the U.S. population) were evaluated for code reform zoning and 11 active living markers. County-aggregated zoning measures were created for linking with five adult PA behaviors obtained from the 2011 Behavioral Risk Factor Surveillance System controlling for individual and county sociodemographics. Zoning elements most associated with adult PA included requirements for mixed use, active and passive recreation, bike parking/street furniture, and bike-pedestrian trails/paths. This study provides new insights as to the role that zoning can play in facilitating adult PA. PMID:27587898

  7. Age 55 or better: active adult communities and city planning.

    PubMed

    Trolander, Judith Ann

    2011-01-01

    Active adult, age-restricted communities are significant to urban history and city planning. As communities that ban the permanent residence of children under the age of nineteen with senior zoning overlays, they are unique experiments in social planning. While they do not originate the concept of the common interest community with its shared amenities, the residential golf course community, or the gated community, Sun Cities and Leisure Worlds do a lot to popularize those physical planning concepts. The first age-restricted community, Youngtown, AZ, opened in 1954. Inspired by amenity-rich trailer courts in Florida, Del Webb added the “active adult” element when he opened Sun City, AZ, in 1960. Two years later, Ross Cortese opened the first of his gated Leisure Worlds. By the twenty-first century, these “lifestyle” communities had proliferated and had expanded their appeal to around 18 percent of retirees, along with influencing the design of intergenerational communities. PMID:22175080

  8. Impression Creep Behavior of a Cast AZ91 Magnesium Alloy

    NASA Astrophysics Data System (ADS)

    Kabirian, F.; Mahmudi, R.

    2009-01-01

    The creep behavior of the cast AZ91 magnesium alloy was investigated by impression testing. The tests were carried out under constant punching stress in the range 100 to 650 MPa, corresponding to 0.007 ≤ σ imp/ G ≤ 0.044, at temperatures in the range 425 to 570 K. Assuming a power-law relationship between the impression velocity and stress, depending on the testing temperature, stress exponents of 4.2 to 6.0 were obtained. When the experimental creep rates were normalized to the grain size and effective diffusion coefficient, a stress exponent of approximately 5 was obtained, which is in complete agreement with stress exponents determined by the conventional creep testing of the same material reported in the literature. Calculation of the activation energy showed a slight decrease in the activation energy with increasing stress such that the creep-activation energy of 122.9 kJ/mol at σ imp/ G = 0.020 decreases to 94.0 kJ/mol at σ imp/ G = 0.040. Based on the obtained stress exponents and activation energy data, it is proposed that dislocation climb is the controlling creep mechanism. However, due to the decreasing trend of creep-activation energy with stress, it is suggested that two parallel mechanisms of lattice and pipe-diffusion-controlled dislocation climb are competing. To elucidate the contribution of each mechanism to the overall creep deformation, the creep rates were calculated based on the effective activation energy. This yielded a criterion that showed that, in the high-stress regimes, the experimental activation energies fall in the range in which the operative creep mechanism is dislocation climb controlled by dislocation pipe diffusion. In the low-stress regime, however, the lattice-diffusion dislocation climb is dominant.

  9. The fate and transport of nitroglycerin in the unsaturated zone at active and legacy anti-tank firing positions

    NASA Astrophysics Data System (ADS)

    Bordeleau, Geneviève; Martel, Richard; Ampleman, Guy; Thiboutot, Sonia; Poulin, Isabelle

    2012-11-01

    The environmental fate of nitroglycerin (NG) in the unsaturated zone was evaluated in the context of double-base propellant residue deposition at anti-tank training ranges. Fresh propellant residues were collected during live anti-tank training. Surface soils, sub-surface soils and water samples from the unsaturated zone were collected at an active anti-tank range, and at a legacy site where NG-based propellants have been used. Results show that the residues are composed of intact propellant particles, as well as small quantities of NG, dinitroglycerin (DNG) and nitrate which are rapidly dissolved by precipitation, resulting in sporadic pulses of those compounds in water from the unsaturated zone after rain/snow melt events. The dissolved NG and DNG can be progressively degraded in the unsaturated zone, releasing nitrate as an end-product. Over a period of several years, small propellant particles located at the soil surface can be carried downward through the soil pore system by infiltration water, which explains the presence of NG in sub-surface soils at the legacy site, more than 35 years after site closure. NG is no longer leached from these old particles, therefore the detection of NG in sub-surface soils does not signify that groundwater is at risk of contamination by NG.

  10. The fate and transport of nitroglycerin in the unsaturated zone at active and legacy anti-tank firing positions.

    PubMed

    Bordeleau, Geneviève; Martel, Richard; Ampleman, Guy; Thiboutot, Sonia; Poulin, Isabelle

    2012-11-01

    The environmental fate of nitroglycerin (NG) in the unsaturated zone was evaluated in the context of double-base propellant residue deposition at anti-tank training ranges. Fresh propellant residues were collected during live anti-tank training. Surface soils, sub-surface soils and water samples from the unsaturated zone were collected at an active anti-tank range, and at a legacy site where NG-based propellants have been used. Results show that the residues are composed of intact propellant particles, as well as small quantities of NG, dinitroglycerin (DNG) and nitrate which are rapidly dissolved by precipitation, resulting in sporadic pulses of those compounds in water from the unsaturated zone after rain/snow melt events. The dissolved NG and DNG can be progressively degraded in the unsaturated zone, releasing nitrate as an end-product. Over a period of several years, small propellant particles located at the soil surface can be carried downward through the soil pore system by infiltration water, which explains the presence of NG in sub-surface soils at the legacy site, more than 35 years after site closure. NG is no longer leached from these old particles, therefore the detection of NG in sub-surface soils does not signify that groundwater is at risk of contamination by NG. PMID:23047138

  11. 241-AZ-101 pump removal trough analysis

    SciTech Connect

    Coverdell, B.L.

    1995-10-17

    As part of the current Hanford mission of environmental cleanup, various long length equipment must be removed from highly radioactive waste tanks. The removal of equipment will utilize portions of the Equipment Removal System for Project W320 (ERS-W320), specifically the 50 ton hydraulic trailer system. Because the ERS-W320 system was designed to accommodate much heavier equipment it is adequate to support the dead weight of the trough, carriage and related equipment for 241AZ101 pump removal project. However, the ERS-W320 components when combined with the trough and its` related components must also be analyzed for overturning due to wind loads. Two troughs were designed, one for the 20 in. diameter carriage and one for the 36 in. diameter carriage. A proposed 52 in. trough was not designed and, therefore is not included in this document. In order to fit in the ERS-W320 strongback the troughs were design with the same widths. Structurally, the only difference between the two troughs is that more material was removed from the stiffener plates on the 36 in trough. The reduction in stiffener plate material reduces the allowable load. Therefore, only the 36 in. trough was analyzed.

  12. Ultrasonic Examination of Double-Shell Tank 241-AZ-101. Examination Completed July 2007.

    SciTech Connect

    Pardini, Allan F.; Weier, Dennis R.

    2007-08-12

    AREVA NC Inc. (AREVA), under a contract from CH2M Hill Hanford Group (CH2M Hill), has performed an ultrasonic examination of selected portions of Double-Shell Tank 241-AZ-101. PNNL is responsible for preparing a report(s) that describes the results of the AREVA ultrasonic examinations. The purpose of this examination was to provide information that could be used to evaluate the integrity of the wall of the primary tank. The requirements for the ultrasonic examination of Tank 241-AZ-101 were to detect, characterize (identify, size, and locate), and record measurements made of any wall thinning, pitting, or cracks that might be present in the wall of the primary tank. Any measurements that exceed the requirements set forth in the Engineering Task Plan (ETP), RPP-Plan-27202 (Jensen 2005) and summarized on page 1 of this document, are to be reported to CH2M HILL and the Pacific Northwest National Laboratory (PNNL) for further evaluation. Under the contract with CH2M HILL, all data is to be recorded on electronic media and paper copies of all measurements are provided to PNNL for third-party evaluation. PNNL is responsible for preparing a report(s) that describes the results of the AREVA ultrasonic examinations. The results of the examination of Tank 241-AZ-101 have been evaluated by PNNL personnel. The ultrasonic examination consisted of two vertical 15-in.-wide scan paths over the entire height of the tank and the heat-affected zone (HAZ) of five vertical welds and one horizontal weld from Riser 89. The examination also included two vertical 15-in.-wide scan paths over the entire height of the tank from Riser 90. The examination was performed to detect any wall thinning, pitting, or cracking in the primary tank wall.

  13. On the seismic activity of the Malibu Coast Fault Zone, and other ethical problems in engineering geoscience

    SciTech Connect

    Cronin, V.S. . Geosciences Dept.)

    1992-01-01

    The Malibu Coast Fault Zone (MCFZ) merges eastward with the active Santa Monica, Hollywood, Raymond Hill, Sierra Madre, and Cucamonga Faults of the central Transverse Ranges. West of Point Dume, the MCFZ extends offshore to join the active Santa Cruz Island Fault. Active microearthquake seismicity along the MCFZ trend indicates that it is seismogenic. Focal mechanism solutions for several of these earthquakes indicate thrusting along faults with the same orientation as the MCFZ. The geomorphology of the MCFZ is consistent with the interpretation that the MCFZ is active. Scarps in unconsolidated sands along the continental shelf just south of Malibu indicate recent offset. In the Santa Monica Mountains, late Tertiary and Quaternary marine sedimentary strata are exposed on the hanging-wall side of the MCFZ, indicating active uplift of the Santa Monica Mountains. Given the other indicators of fault activity, the trench studies that must still be undertaken across the MCFZ are more likely to establish the chronology of recent displacement along the MCFZ than to indicate that the fault is not active. It has been suggested that the MCFZ has not yet been formally recognized as an active, seismogenic fault zone because of the expected loss of property value should the MCFZ be designated an active fault. Geoscientists fear being held liable for loss of property value, even though their assessment of fault activity may be scientifically valid. What are the ethical responsibilities of geoscientists involved in seismic risk assessment along the MCFZ Are political or financial considerations valid criteria to use in assessing the activity of a fault These are not abstract questions of geoethics, because the lives and properties of countless people are potentially at risk.

  14. Caustic leaching of composite AZ-101/AZ-102 Hanford tank sludge

    SciTech Connect

    Rapko, B.M.; Wagner, M.J.

    1997-07-01

    To reduce the quantity (and hence the cost) of glass canisters needed for disposing of high-level radioactive wastes from the Hanford tank farms, pretreatment processes are needed to remove as much nonradioactive material as possible. This report describes the results of a laboratory-scale caustic leaching test performed on a composite derived from a combination of 241-AZ-101 and 241-AZ-102 Hanford Tank sludges. The goals of this FY 1996 test were to evaluate the effectiveness of caustic leaching on removing key components from the sludge and to evaluate the effectiveness of varying the free-hydroxide concentrations by incrementally increasing the free hydroxide concentration of the leach steps up to 3 {und M} free hydroxide. Particle-size analysis of the treated and untreated sludge indicated that the size and range of the sludge particles remained essentially unchanged by the caustic leaching treatment. Both before and after caustic leaching, a particle range of 0.2 {micro}m to 50 {micro}m was observed, with mean particle diameters of 8.5 to 9 {micro}m based on the volume distribution and mean particle diameters of 0.3 to 0.4 {micro}m based on the number distribution.

  15. Tank 241-AZ-102 Privatization Push Mode Core Sampling and Analysis Plan

    SciTech Connect

    RASMUSSEN, J.H.

    1999-08-02

    This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for samples obtained from tank 241-AZ-102. The purpose of this sampling event is to obtain information about the characteristics of the contents of 241-AZ-102 required to satisfy the Data Quality Objectives For TWRS Privatization Phase I: Confirm Tank TIS An Appropriate Feed Source For High-Level Waste Feed Batch X(HLW DQO) (Nguyen 1999a), Data Quality Objectives For TWRS Privatization Phase 1: Confirm Tank TIS An Appropriate Feed Source For Low-Activity Waste Feed Batch X (LAW DQO) (Nguyen 1999b), Low Activity Waste and High Level Waste Feed Data Quality Objectives (L&H DQO) (Patello et al. 1999) and Characterization Data Needs for Development, Design, and Operation of Retrieval Equipment Developed through the Data Quality Objective Process (Equipment DQO) (Bloom 1996). The Tank Characterization Technical Sampling Basis document (Brown et al. 1998) indicates that these issues, except the Equipment DQO apply to tank 241-AZ-102 for this sampling event. The Equipment DQO is applied for shear strength measurements of the solids segments only. Poppiti (1999) requires additional americium-241 analyses of the sludge segments. Brown et al. (1998) also identify safety screening, regulatory issues and provision of samples to the Privatization Contractor(s) as applicable issues for this tank. However, these issues will not be addressed via this sampling event. Reynolds et al. (1999) concluded that information from previous sampling events was sufficient to satisfy the safety screening requirements for tank 241 -AZ-102. Push mode core samples will be obtained from risers 15C and 24A to provide sufficient material for the chemical analyses and tests required to satisfy these data quality objectives. The 222-S Laboratory will extrude core samples, composite the liquids and solids, perform chemical analyses

  16. Preliminary assessment of the nuclide migration from the activation zone around the proposed Spallation Neutron Source facility

    SciTech Connect

    Dole, L.R.

    1998-09-01

    The purpose of this study is to investigate the potential impacts of migrating radionuclides from the activation zone around the proposed Spallation Neutron Source (SNS). Using conservatively high estimates of the potential inventory of radioactive activation products that could form in the proposed compacted-soil shield berm around an SNS facility on the Oak Ridge Reservation (ORR), a conservative, simplified transport model was used to estimate the potential worst-case concentrations of the 12 long-lived isotopes in the groundwater under a site with the hydrologic characteristics of the ORR.

  17. Long-Term Uplift in the Altiplano-Puna Neovolcanic Zone: Evidence of an Active Magmatic Diapir?

    NASA Astrophysics Data System (ADS)

    Fialko, Y.; Pearse, J.

    2012-12-01

    We present InSAR observations of a long-term uplift in the Altiplano-Puna neovolcanic zone (central Andes, South America). Previous observations revealed a a massive Ultra Low Velocity Zone (ULVZ) at depth of 17-19 km (Zandt et al., 2003), and surface deformation that was attributed to Uturuncu, a dormant volcano in the middle of the Altiplano-Puna neovolcanic zone (Pritchard and Simons, 2002). Our time series analysis of combined data from different sensors (ERS-1/2 and ENVISAT), satellite tracks, and observation modes (fine beam and ScanSAR) reveals that the central uplift has persisted at a nearly constant rate of ~1 cm/yr over the last two decades, and is surrounded by a broad zone of subsidence. We use the satellite line-of-sight velocities from different look directions to constrain the depth and geometry of the inferred sources of magmatic unrest. Inversions based on elastic half-space models indicate that the inflation source is located well below the brittle-ductile transition, and likely resides at the depth of the seismically imaged ULVZ. We investigated the effects of inelastic deformation in the ambient crust using finite element models. The models incorporated laboratory-derived rheologies of the ambient crust, and geotherms appropriate for an active neo-volcanic zone such as the one in the Altiplano-Puna province. Based on a large number of numerical simulations constrained by the observed surface velocities, we conclude that the ongoing uplift and peripheral subsidence result from a large mid-crustal diapir fed by a partially molten source region in the middle crust. The observed pattern of surface deformation due to the Altiplano-Puna ULVZ is remarkably similar to that due to the Socorro Magma Body (SMB) in central New Mexico, USA (Pearse and Fialko, 2010), suggesting a common process. mosaic of the mean LOS velocity showing uplift and peripheral subsidence due to the inferred mid-crustal diapir.

  18. Assessment of Debris Flow Hazards, North Mountain, Phoenix, AZ

    NASA Astrophysics Data System (ADS)

    Reavis, K. J.; Wasklewicz, T. A.

    2014-12-01

    Urban sprawl in many western U.S. cities has expanded development onto alluvial fans. In the case of metropolitan Phoenix, AZ (MPA), urban sprawl has led to an exponential outward growth into surrounding mountainous areas and onto alluvial fans. Building on alluvial fans places humans at greater risk to flooding and debris flow hazards. Recent research has shown debris flows often supply large quantities of material to many alluvial fans in MPA. However, the risk of debris flows to built environments is relatively unknown. We use a 2D debris flow modeling approach, aided by high-resolution airborne LiDAR and terrestrial laser scanning (TLS) topographic data, to examine debris flow behavior in a densely populated portion of the MPA to assess the risk and vulnerability of debris flow damage to the built infrastructure. A calibrated 2D debris flow model is developed for a "known" recent debris flow at an undeveloped site in MPA. The calibrated model and two other model scenarios are applied to a populated area with historical evidence of debris flow activity. Results from the modeled scenarios show evidence of debris flow damage to houses built on the alluvial fan. Debris flow inundation is also evident on streets on the fan. We use housing values and building damage to estimate the costs assocaited with various modeled debris flow scenarios.

  19. Dynamic fracturing by successive coseismic loadings leads to pulverization in active fault zones

    NASA Astrophysics Data System (ADS)

    Aben, F. M.; Doan, M.-L.; Mitchell, T. M.; Toussaint, R.; Reuschlé, T.; Fondriest, M.; Gratier, J.-P.; Renard, F.

    2016-04-01

    Previous studies show that pulverized rocks observed along large faults can be created by single high-strain rate loadings in the laboratory, provided that the strain rate is higher than a certain pulverization threshold. Such loadings are analogous to large seismic events. In reality, pulverized rocks have been subject to numerous seismic events rather than one single event. Therefore, the effect of successive "milder" high-strain rate loadings on the pulverization threshold is investigated by applying loading conditions below the initial pulverization threshold. Single and successive loading experiments were performed on quartz-monzonite using a Split Hopkinson Pressure Bar apparatus. Damage-dependent petrophysical properties and elastic moduli were monitored by applying incremental strains. Furthermore, it is shown that the pulverization threshold can be reduced by successive "milder" dynamic loadings from strain rates of ~180 s-1 to ~90 s-1. To do so, it is imperative that the rock experiences dynamic fracturing during the successive loadings prior to pulverization. Combined with loading conditions during an earthquake rupture event, the following generalized fault damage zone structure perpendicular to the fault will develop: furthest from the fault plane, there is a stationary outer boundary that bounds a zone of dynamically fractured rocks. Closer to the fault, a pulverization boundary delimits a band of pulverized rock. Consecutive seismic events will cause progressive broadening of the band of pulverized rocks, eventually creating a wider damage zone observed in mature faults.

  20. 14 CFR 93.305 - Flight-free zones and flight corridors.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Flight-free zones and flight corridors. 93... (CONTINUED) AIR TRAFFIC AND GENERAL OPERATING RULES SPECIAL AIR TRAFFIC RULES Special Flight Rules in the Vicinity of Grand Canyon National Park, AZ § 93.305 Flight-free zones and flight corridors. Except in...

  1. Electroless Nickel Phosphorus Plating on AZ31

    NASA Astrophysics Data System (ADS)

    Shartal, Kh. M.; Kipouros, G. J.

    2009-04-01

    One of the major drawbacks to using magnesium parts in automotive applications is poor corrosion resistance, which can be improved with a nickel-boron coating placed on a nickel-phosphorus coating, which, in turn, is placed on a phosphate-permanganate conversion-coating layer produced on the magnesium alloy AZ31. This work reports on the determination of the optimum kinetic parameters for producing a coherent nickel-phosphorus coating using an electroless-procedure phosphate-permanganate conversion-coating layer and for studying the effects of the experimental variables of the electroless plating process on the phosphorus content, surface morphology, and structure of the electroless nickel-phosphorus (EN-P) coatings produced. Measurements of the plating rate as a function of experimental variables such as the compositions of the plating bath constituents, temperature, and pH were implemented using the weight-gain method; the phosphorus content of the EN-P coatings was measured using energy-dispersive spectroscopy (EDS) analysis. The surface morphology of the coating was examined using a scanning electron microscope (SEM); X-ray diffraction (XRD) was used to characterize the structure of each coating. An empirical rate law was determined for EN-P plating on a phosphate-permanganate conversion coating. It is found that the deposition rate of the EN-P coating increases by increasing the deposition temperature, the concentration of free nickel ions, and the concentration of hypophosphite ions in the plating bath. In addition, the deposition rate decreases by increasing both the plating bath pH and the concentration of citric acid in the plating bath.

  2. Corrosion fatigue behaviors of two biomedical Mg alloys - AZ91D and WE43 - In simulated body fluid.

    PubMed

    Gu, X N; Zhou, W R; Zheng, Y F; Cheng, Y; Wei, S C; Zhong, S P; Xi, T F; Chen, L J

    2010-12-01

    Magnesium alloys have been recently developed as biodegradable implant materials, yet there has been no study concerning their corrosion fatigue properties under cyclic loading. In this study the die-cast AZ91D (A for aluminum 9%, Z for zinc 1% and D for a fourth phase) and extruded WE43 (W for yttrium 4%, E for rare earth mischmetal 3%) alloys were chosen to evaluate their fatigue and corrosion fatigue behaviors in simulated body fluid (SBF). The die-cast AZ91D alloy indicated a fatigue limit of 50MPa at 10⁷ cycles in air compared to 20MPa at 10⁶ cycles tested in SBF at 37°C. A fatigue limit of 110MPa at 10⁷ cycles in air was observed for extruded WE43 alloy compared to 40MPa at 10⁷ cycles tested in SBF at 37°C. The fatigue cracks initiated from the micropores when tested in air and from corrosion pits when tested in SBF, respectively. The overload zone of the extruded WE43 alloy exhibited a ductile fracture mode with deep dimples, in comparison to a brittle fracture mode for the die-cast AZ91D. The corrosion rate of the two experimental alloys increased under cyclic loading compared to that in the static immersion test. PMID:20656074

  3. Continuous monitoring of an active fault in a plate suture zone: a creepmeter study of the Chihshang Fault, eastern Taiwan

    NASA Astrophysics Data System (ADS)

    Lee, J.-C.; Angelier, J.; Chu, H.-T.; Hu, J.-C.; Jeng, F.-S.

    2001-04-01

    Data from continuously monitored creepmeters across the active Chihshang Fault in eastern Taiwan are presented. The Chihshang Fault is an active segment of the Longitudinal Valley Fault, the main suture between the converging Philippine and Eurasian plates in Taiwan. Since the 1951 earthquake (Mw=7.0), no earthquake larger than magnitude 6.0 occurred in the Chihshang area. At least during the last 20 years, the Chihshang Fault underwent a steady creep movement, resulting in numerous fractures at the surface. Five creepmeters were installed in 1998 at two sites, Tapo and Chinyuan, within the Chihshang active fault zone. One-year results (from August 1998 to July 1999) show a horizontal shortening of 19.4±0.3 mm and 17.3±0.7 mm, at Tapo and Chinyuan, respectively. These annual shortening rates are in a good agreement with other estimates of strain rate independently obtained from geodetic measurements and geological site investigation. The creepmeter measurements were made on a daily basis, providing accurate information on the previously unknown evolution of creep during the year. The records of fault creep at the Tapo site thus revealed close seasonal correlation with average rainfall: the period of high creep rate coincides with the wet season, whereas that of low creep rate coincides with the dry season. Also, in comparison with the Tapo site, the creep behaviour as a function of time is complex at the Chinyuan site. Possible factors of irregularity are under investigation (thermal effect acting on the concrete basement of the creepmeters, earth tide effect, water table variations in a nearby rice field, and rainfall). The comparison between GPS measurements across the Longitudinal Valley (31 mm/year of horizontal displacement) and the creepmeter measurement across the Chihshang Fault zone (17-19 mm/year of horizontal displacement) suggests that there exists other shortening deformation across the active fault zone in addition to those we have measured from the

  4. Toll-like receptor stimulation in splenic marginal zone lymphoma can modulate cell signaling, activation and proliferation

    PubMed Central

    Fonte, Eleonora; Agathangelidis, Andreas; Reverberi, Daniele; Ntoufa, Stavroula; Scarfò, Lydia; Ranghetti, Pamela; Cutrona, Giovanna; Tedeschi, Alessandra; Xochelli, Aliki; Caligaris-Cappio, Federico; Ponzoni, Maurilio; Belessi, Chrysoula; Davis, Zadie; Piris, Miguel A.; Oscier, David; Ghia, Paolo; Stamatopoulos, Kostas; Muzio, Marta

    2015-01-01

    Recent studies on splenic marginal zone lymphoma identified distinct mutations in genes belonging to the B-cell receptor and Toll-like receptor signaling pathways, thus pointing to their potential implication in the biology of the disease. However, limited data is available regarding the exact role of TLRs. We aimed at characterizing the expression pattern of TLRs in splenic marginal zone lymphoma cells and their functional impact on the activation, proliferation and viability of malignant cells in vitro. Cells expressed significant levels of TLR1, TLR6, TLR7, TLR8, TLR9 and TLR10 mRNA; TLR2 and TLR4 showed a low, variable pattern of expression among patients whereas TLR3 and TLR5 mRNAs were undetectable; mRNA specific for TLR signaling molecules and adapters was also expressed. At the protein level, TLR1, TLR6, TLR7, TLR9 and TLR10 were detected. Stimulation of TLR1/2, TLR2/6 and TLR9 with their respective ligands triggered the activation of IRAK kinases, MAPK and NF-κB signaling pathways, and the induction of CD86 and CD25 activation molecules, although in a heterogeneous manner among different patient samples. TLR-induced activation and cell viability were also inhibited by a specific IRAK1/4 inhibitor, thus strongly supporting the specific role of TLR signaling in these processes. Furthermore, TLR2/6 and TLR9 stimulation also significantly increased cell proliferation. In conclusion, we demonstrate that splenic marginal zone lymphoma cells are equipped with functional TLR and signaling molecules and that the stimulation of TLR1/2, TLR2/6 and TLR9 may play a role in regulating disease pathobiology, likely promoting the expansion of the neoplastic clone. PMID:26294727

  5. Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats

    PubMed Central

    Zhou, Jian; Liu, Zhao; Yu, Jia; Han, Xin; Fan, Songhua; Shao, Weihua; Chen, Jianjun; Qiao, Rui

    2016-01-01

    Background: While stressful events are recognized as an important cause of major depressive disorder, some individuals exposed to life stressors maintain normal psychological functioning. The molecular mechanism(s) underlying this phenomenon remain unclear. Abnormal transmission and plasticity of hippocampal synapses have been implied to play a key role in the pathoetiology of major depressive disorder. Methods: A chronic mild stress protocol was applied to separate susceptible and unsusceptible rat subpopulations. Proteomic analysis using an isobaric tag for relative and absolute quantitation coupled with tandem mass spectrometry was performed to identify differential proteins in enriched hippocampal synaptic junction preparations. Results: A total of 4318 proteins were quantified, and 89 membrane proteins were present in differential amounts. Of these, SynaptomeDB identified 81 (91%) having a synapse-specific localization. The unbiased profiles identified several candidate proteins within the synaptic junction that may be associated with stress vulnerability or insusceptibility. Subsequent functional categorization revealed that protein systems particularly involved in membrane trafficking at the synaptic active zone exhibited a positive strain as potential molecular adaptations in the unsusceptible rats. Moreover, through STRING and immunoblotting analysis, membrane-associated GTP-bound Rab3a and Munc18-1 appear to coregulate syntaxin-1/SNAP25/VAMP2 assembly at the hippocampal presynaptic active zone of unsusceptible rats, facilitating SNARE-mediated membrane fusion and neurotransmitter release, and may be part of a stress-protection mechanism in actively maintaining an emotional homeostasis. Conclusions: The present results support the concept that there is a range of potential protein adaptations in the hippocampal synaptic active zone of unsusceptible rats, revealing new investigative targets that may contribute to a better understanding of stress

  6. Fault Activity, Seismicity and GPS Deformation of the Seismic Gap along the Red River Fault Zone (RRFZ) in Yunnan, China

    NASA Astrophysics Data System (ADS)

    Xue-Ze, Wen; Shengli, Ma; Fang, Du; Feng, Long

    2016-04-01

    Along the middle segment of the NW-trending and dextral-slip Red River fault zone (RRFZ), also the Honghe fault zone, Yunnan, China, there has been little of modern seismicity since the 1970's. Some Chinese researchers believed that this fault segment is inactive in the late Quaternary. However, more and more evidence shows that the middle segment of RRFZ is geologically-active in the late Quaternary, even is a Holocene-active one with evidence of paleo-earthquakes occurring. Our study suggests that along the fault segment there has been no any major earthquake occurring for over 500 years at least, and a large-scale seismic gap, the Honghe seismic gap, have formed there. On the modern seismicity, the middle segment of RRFZ has presented as a fault portion without or with very few small earthquakes occurring since the 1980's, but surrounded by several areas with low b-values, suggesting relatively high stress having built-up there. Also, GPS deformation analysis suggests that this fault segment has tightly locked already. Such tight locking would be associated with the fault geometry: A large-scale restraining bend of about 30°over a distance of ~100 km exists along the main fault trace along RRFZ between Yuanjiang and Yuanyang. However, how such a restraining bend makes the middle segment of RRFZ have tightly locked? How much strain has built up there? Moreover, how about the long-term seismic potential of major earthquake on the middle segment of RRFZ, and on some secondary active faults of the two sides of the segment, especially on the parallel faults Chuxiong, Qujiang and Shiping. All these are issues we want to study further. Keywords: Red River Fault Zone, Seismic Gap, Fault Activity, Seismicity, GPS Deformation

  7. Toll-like receptor stimulation in splenic marginal zone lymphoma can modulate cell signaling, activation and proliferation.

    PubMed

    Fonte, Eleonora; Agathangelidis, Andreas; Reverberi, Daniele; Ntoufa, Stavroula; Scarfò, Lydia; Ranghetti, Pamela; Cutrona, Giovanna; Tedeschi, Alessandra; Xochelli, Aliki; Caligaris-Cappio, Federico; Ponzoni, Maurilio; Belessi, Chrysoula; Davis, Zadie; Piris, Miguel A; Oscier, David; Ghia, Paolo; Stamatopoulos, Kostas; Muzio, Marta

    2015-11-01

    Recent studies on splenic marginal zone lymphoma identified distinct mutations in genes belonging to the B-cell receptor and Toll-like receptor signaling pathways, thus pointing to their potential implication in the biology of the disease. However, limited data is available regarding the exact role of TLRs. We aimed at characterizing the expression pattern of TLRs in splenic marginal zone lymphoma cells and their functional impact on the activation, proliferation and viability of malignant cells in vitro. Cells expressed significant levels of TLR1, TLR6, TLR7, TLR8, TLR9 and TLR10 mRNA; TLR2 and TLR4 showed a low, variable pattern of expression among patients whereas TLR3 and TLR5 mRNAs were undetectable; mRNA specific for TLR signaling molecules and adapters was also expressed. At the protein level, TLR1, TLR6, TLR7, TLR9 and TLR10 were detected. Stimulation of TLR1/2, TLR2/6 and TLR9 with their respective ligands triggered the activation of IRAK kinases, MAPK and NF-κB signaling pathways, and the induction of CD86 and CD25 activation molecules, although in a heterogeneous manner among different patient samples. TLR-induced activation and cell viability were also inhibited by a specific IRAK1/4 inhibitor, thus strongly supporting the specific role of TLR signaling in these processes. Furthermore, TLR2/6 and TLR9 stimulation also significantly increased cell proliferation. In conclusion, we demonstrate that splenic marginal zone lymphoma cells are equipped with functional TLR and signaling molecules and that the stimulation of TLR1/2, TLR2/6 and TLR9 may play a role in regulating disease pathobiology, likely promoting the expansion of the neoplastic clone. PMID:26294727

  8. Active and passive-source imaging of the Cascadia subduction zone using both onshore and offshore data

    NASA Astrophysics Data System (ADS)

    Janiszewski, H. A.; Abers, G. A.; Carton, H. D.; Webb, S. C.; Gaherty, J. B.; Trehu, A. M.

    2013-12-01

    The Cascadia subduction zone is characterized by the subduction of young lithosphere with relatively little seismicity, despite evidence of prehistoric earthquakes, and a thick incoming sediment section that feeds the plate interface. It has been suggested that the thrust zone forms a high-porosity channel of near-lithostatic pressure to 40 km depth, but stronger metasediments may also explain many observations. To test these hypotheses, we analyze new data and integrate results from both active and passive-source seismic studies of Cascadia that sample the interplate thrust zone. In June-July 2012, fifteen seismometers were deployed in Washington from the coast to 140 km inland to record airguns from the R/V Langseth along a linear trench-perpendicular profile. We also analyze broadband data from the coincident onshore CAFE (2006-08) broadband high-density array, which provided high-resolution receiver function images of the downgoing plate, and with the offshore Grays Harbor array of the Cascadia Initiative (CI). In the active-source data, arrivals are observed at up to 140 km offset from the stations, the farthest of which are likely turning waves that travel in the slab mantle. Signals from all but the farthest inland stations are dominated by strong reverberating signals at 20-90 km offset. Preliminary calculations indicate that some of these signals have apparent velocity and timing consistent with waves that reflect off the plate interface or just above it. Bounce points for these rays map a zone of high reflectivity extending ~15-20 km on either side of the coastline. Some aspects of the signals may indicate an origin on or near the plate boundary. In addition, these reflections directly underlie CAFE stations where receiver functions have been obtained on land and the CI broadband stations where receiver functions are being obtained offshore, allowing for direct comparison and integration of all three datasets. The CI stations present several challenges

  9. Polishing-assisted galvanic corrosion in the dissimilar friction stir welded joint of AZ31 magnesium alloy to 2024 aluminum alloy

    SciTech Connect

    Liu, C.; Chen, D.L. Bhole, S.; Cao, X.; Jahazi, M.

    2009-05-15

    Galvanic corrosion of a dissimilar friction stir welded 2024-T3 Al/AZ31B-H24 Mg joint prepared using a water-based and a non-water-based polishing solution was characterized. Microstructure and the distribution of chemical elements were analyzed using optical microscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy. The stir zone polished using water-based solution was observed to be much more susceptible to galvanic corrosion attack than that obtained using non-water-based polishing solution. The location of corrosion attack was observed in the narrow regions of AZ31 Mg alloy adjacent to Al2024 regions in the stir zone. The occurrence of galvanic corrosion was due to the formation of Mg/Al galvanic couples with a small ratio of anode-to-cathode surface area. The corrosion product was primarily the porous magnesium hydroxide with characteristic microcracks and exhibited a low microhardness value.

  10. Diversity and Dynamics of Active Small Microbial Eukaryotes in the Anoxic Zone of a Freshwater Meromictic Lake (Pavin, France)

    PubMed Central

    Lepère, Cécile; Domaizon, Isabelle; Hugoni, Mylène; Vellet, Agnès; Debroas, Didier

    2016-01-01

    Microbial eukaryotes play a crucial role in ecosystem functioning and oxygen is considered to be one of the strongest barriers against their local dispersal. However, diversity of microbial eukaryotes in freshwater habitats with oxygen gradients has previously received very little attention. We applied high-throughput sequencing (V4 region of the 18S rRNA gene) in conjunction with quantitative PCR (DNA and RNA) and fluorescent in situ hybridization (FISH) analyses, to provide an unique spatio-temporal analysis of microbial eukaryotes diversity and potential activity in a meromictic freshwater lake (lake Pavin). This study revealed a high genetic diversity of unicellular eukaryotes in the permanent anoxic zone of lake Pavin and allowed the discrimination of active vs. inactive components. Forty-two percent of the OTUs (Operational Taxonomic Units) are exclusively present in the monimolimnion, where Alveolata (Ciliophora and Dinophyceae) and Fungi (Dikarya and Chytrids) are the most active phyla and are probably represented by species capable of anaerobic metabolism. Pigmented eukaryotes (Haptophyceae and Chlorophyceae) are also present and active in this zone, which opens up questions regarding their metabolism. PMID:26904006

  11. X-ray diffraction indicates that active cross-bridges bind to actin target zones in insect flight muscle.

    PubMed

    Tregear, R T; Edwards, R J; Irving, T C; Poole, K J; Reedy, M C; Schmitz, H; Towns-Andrews, E; Reedy, M K

    1998-03-01

    We report the first time-resolved study of the two-dimensional x-ray diffraction pattern during active contraction in insect flight muscle (IFM). Activation of demembranated Lethocerus IFM was triggered by 1.5-2.5% step stretches (risetime 10 ms; held for 1.5 s) giving delayed active tension that peaked at 100-200 ms. Bundles of 8-12 fibers were stretch-activated on SRS synchrotron x-ray beamline 16.1, and time-resolved changes in diffraction were monitored with a SRS 2-D multiwire detector. As active tension rose, the 14.5- and 7.2-nm meridionals fell, the first row line dropped at the 38.7 nm layer line while gaining a new peak at 19.3 nm, and three outer peaks on the 38.7-nm layer line rose. The first row line changes suggest restricted binding of active myosin heads to the helically preferred region in each actin target zone, where, in rigor, two-headed lead bridges bind, midway between troponin bulges that repeat every 38.7 nm. Halving this troponin repeat by binding of single active heads explains the intensity rise at 19.3 nm being coupled to a loss at 38.7 nm. The meridional changes signal movement of at least 30% of all myosin heads away from their axially ordered positions on the myosin helix. The 38.7- and 19.3-nm layer line changes signal stereoselective attachment of 7-23% of the myosin heads to the actin helix, although with too little ordering at 6-nm resolution to affect the 5.9-nm actin layer line. We conclude that stretch-activated tension of IFM is produced by cross-bridges that bind to rigor's lead-bridge target zones, comprising < or = 1/3 of the 75-80% that attach in rigor. PMID:9512040

  12. 78 FR 22512 - Foreign-Trade Zone (FTZ) 225-Springfield, Missouri; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-16

    ... Production Activity; General Dynamics Ordnance and Tactical Systems Munitions Services (Demilitarization of... notification of proposed production activity to the FTZ Board on behalf of General Dynamics Ordnance...

  13. Fiber Laser Welded AZ31 Magnesium Alloy: The Effect of Welding Speed on Microstructure and Mechanical Properties

    NASA Astrophysics Data System (ADS)

    Chowdhury, S. H.; Chen, D. L.; Bhole, S. D.; Powidajko, E.; Weckman, D. C.; Zhou, Y.

    2012-06-01

    This study was aimed at characterizing microstructural change and evaluating tensile and fatigue properties of fiber laser welded AZ31B-H24 Mg alloy with special attention to the effect of welding speed. Laser welding led to the formation of equiaxed dendrites in the fusion zone and columnar dendrites near the fusion zone boundary along with divorced eutectic Mg17Al12 particles and recrystallized grains in the heat-affected zone. The lowest hardness across the weld appeared in the fusion zone. Although the yield strength, ductility, and fatigue life decreased, the hardening capacity increased after laser welding, with a joint efficiency reaching about 90 pct. A higher welding speed resulted in a narrower fusion zone, smaller grain size, higher yield strength, and longer fatigue life, as well as a slightly lower strain-hardening capacity mainly because of the smaller grain sizes. Tensile fracture occurred in the fusion zone, whereas fatigue failure appeared essentially in between the heat-affected zone and the fusion zone. Fatigue cracks initiated from the near-surface welding defects and propagated by the formation of fatigue striations together with secondary cracks.

  14. The Effect of SiC Particle Addition During FSW on Microstructure and Mechanical Properties of AZ31 Magnesium Alloy

    NASA Astrophysics Data System (ADS)

    Abbasi, M.; Abdollahzadeh, A.; Bagheri, B.; Omidvar, H.

    2015-12-01

    Welding and joining of magnesium alloys exert a profound effect on magnesium application expansion, especially in ground and air transportations where large-size, complex components are required. Due to specific physical properties of magnesium, its welding requires great control. In general, the solid-state nature of friction stir welding (FSW) process has been found to produce a low concentration of defects. In the current research, specimens from AZ31 magnesium alloy were welded together using the friction stir process with previously inserted SiC powder particles in the nugget zone. In other words, during the FSW process, the pre-placed SiC particles were stirred throughout the nugget zone of the weld. The results indicated that proper values of rotation and translation speeds led to good appearance of weld zone and suitable distribution of SiC particles producing increased weld strength. The comparison of the microstructures and mechanical properties of FS-welded AZ31 with those of FS-welded one using pre-placed SiC particles showed that the addition of SiC particles decreased the grain size and increased the strength and the formability index.

  15. The manipulation of auxin in the abscission zone cells of Arabidopsis flowers reveals that indoleacetic acid signaling is a prerequisite for organ shedding.

    PubMed

    Basu, Manojit M; González-Carranza, Zinnia H; Azam-Ali, Sayed; Tang, Shouya; Shahid, Ahmad Ali; Roberts, Jeremy A

    2013-05-01

    A number of novel strategies were employed to examine the role of indoleacetic acid (IAA) in regulating floral organ abscission in Arabidopsis (Arabidopsis thaliana). Analysis of auxin influx facilitator expression in β-glucuronidase reporter plants revealed that AUXIN RESISTANT1, LIKE AUX1, and LAX3 were specifically up-regulated at the site of floral organ shedding. Flowers from mutants where individual family members were down-regulated exhibited a reduction in the force necessary to bring about petal separation; however, the effect was not additive in double or quadruple mutants. Using the promoter of a polygalacturonase (At2g41850), active primarily in cells undergoing separation, to drive expression of the bacterial genes iaaL and iaaM, we have shown that it is possible to manipulate auxin activity specifically within the floral organ abscission zone (AZ). Analysis of petal breakstrength reveals that if IAA AZ levels are reduced, shedding takes place prematurely, while if they are enhanced, organ loss is delayed. The At2g41850 promoter was also used to transactivate the gain-of-function AXR3-1 gene in order to disrupt auxin signaling specifically within the floral organ AZ cells. Flowers from transactivated lines failed to shed their sepals, petals, and anthers during pod expansion and maturity, and these organs frequently remained attached to the plant even after silique desiccation and dehiscence had taken place. These observations support a key role for IAA in the regulation of abscission in planta and reveal, to our knowledge for the first time, a requirement for a functional IAA signaling pathway in AZ cells for organ shedding to take place. PMID:23509178

  16. Wetting and Interfacial Characteristics of Mg AZ61 Alloy/Galvanized Steel in Cold Metal Transfer Process

    NASA Astrophysics Data System (ADS)

    Lin, Qiaoli; Yang, Fan; Cao, Rui; Chen, Jianhong; Guo, Tingbiao

    2015-09-01

    The dynamic sessile drop method was used to study the wetting behavior of galvanized steel by molten Mg AZ61 alloy under cold metal transfer condition. The interfacial microstructures were also analyzed by using scanning electron microscope and energy dispersive spectrometry. The observed results showed that the wetting behavior was directly determined by the wire feed speed (or the heat input). The Al-Fe intermetallic layer and Zn-rich zone were observed both at the interface and at the close of triple line. The formations of these interfacial characteristics satisfy the thermodynamic characteristic of Mg-Al/Fe and Mg-Zn/Fe systems.

  17. AZ-101 Mixer Pump Test Qualification Test Procedures (QTP)

    SciTech Connect

    THOMAS, W.K.

    2000-01-10

    Describes the Qualification test procedure for the AZ-101 Mixer Pump Data Acquisition System (DAS). The purpose of this Qualification Test Procedure (QTP) is to confirm that the AZ-101 Mixer Pump System has been properly programmed and hardware configured correctly. This QTP will test the software setpoints for the alarms and also check the wiring configuration from the SIMcart to the HMI. An Acceptance Test Procedure (ATP), similar to this QTP will be performed to test field devices and connections from the field.

  18. Late Quaternary Activity and Seismogenic Potential of the Gonave Microplate: Plantain Garden Strike-Slip Fault Zone of Eastern Jamaica

    NASA Astrophysics Data System (ADS)

    Mann, P.; Prentice, C.; King, W.; Demets, C.; Wiggins-Grandison, M.; Benford, B.

    2008-12-01

    At the longitude of Jamaica, Caribbean (Carib)-North America (Noam) plate motion of 19 ± 2 mm/a is carried by two parallel, left-lateral strike-slip faults, the Oriente fault zone, immediately south of Cuba, and the Enriquillo-Plantain Garden fault zone (EPGFZ), which lies 100-150 km further south. It has been postulated that the lithosphere between these faults constitutes an independent Gonave microplate that has formed in response to the ongoing collision between the leading edge of Carib in Hispaniola and the Bahama carbonate platform. GPS measurements in Jamaica and Hispanola is supportive of the microplate hypothesis and indicates that roughly half of Carib-Noam plate motion (8-14 mm/a) is carried by the EPGFZ of southern Hispaniola and eastern Jamaica. This study applies geomorphic and paleoseismic methods as a direct test of the activity and amount of microplate motion carried on the Plantain Garden fault segment of eastern Hispaniola and how this motion is distributed across a large restraining bend that has formed the island of Jamaica since the late Miocene. The EPFZ curves gently to the northeast and forming a steep mountain front to the Blue Mountains restraining bend with elevations up to 2200 m. Geomorphic fault-related features along the mountain front fault zone include left-laterally deflected rivers and streams, but no small scale features indicative of Holocene activity. River and stream deflections range from 0.1 to 0.5 km. We identified and trenched the most active trace of the mountain front fault at the Morant River where the fault is characterized by a 1.5-m-wide sub-vertical fault zone juxtaposing sheared alluvium and fault Cretaceous basement rocks This section is overlain by a 6-m-thick fluvial terrace. Trenching in the unfaulted terrace immediately overlying the fault trace revealed radiocarbon and OSL ages ranging from 20 to 21 ka that are consistent with a prominent unfaulted alluvial fan along the projection of this fault 1.5 km to

  19. Regional and Detailed Survey for Radon Activities in Soil-Gas and Groundwater in the Okchon Zone, Korea

    NASA Astrophysics Data System (ADS)

    Je, H.-K.; Chon, H.-T.

    2012-04-01

    The Okchon zone in Korea provides a typical example of natural geological materials enriched in potentially toxic elements including uranium which is parent nuclide for radon gas. For the purpose of radon radioactivity risk assessment, making the map of radon risk grade from Okchon zone, regional and detailed field surveys were carried out during 3 years. The study area is located in the central part of Korea, called the Okchon zone (about 5,100 km2), which occur in a 80km wide, northeast-trending belt that extends across the Korean Peninsula. The Okchon zone is underlain by metasedimentary rocks of unknown age that are composed mainly of black slate, phyllite, shale, and limestone. The three research areas (defined as Boeun, Chungju, and Nonsan) for detailed survey were selected from the results of regional survey. Results of detailed radon survey indicated a wide range of radon activities for soil-gases (148-1,843 pCi/L) and ground waters (23-5,540 pCi/L). About 15 percent of soil-gas samples exceeded 1,000 pCi/L and 84 percent of ground water samples exceeded the MCL (maximum contaminant level) of drinking water, 300 pCi/L, which proposed by U.S. Environmental Protection Agency in 1999. For detailed survey, radon activities of soil-gas and ground water were classified as bedrock geology, based on 1/50,000 geological map and field research. For soil-gas measurements, mean values of radon activity from black slate-shale (789 pCi/L) were highest among the other base rocks. And for groundwater measurements, mean value of radon activities were decreased in the order of granite (1,345 pCi/L) > black shale-slate (915 pCi/L) > metasediments (617 pCi/L). Result of indoor radon measurement from detailed survey areas showed that about 50% of houses exceeded the indoor guideline, 4 pCi/L. For the radon risk assessment in indoor environment showed that probability of lung cancer risk from the houses located on the granite base rock (3.0×10-2) was highest among the other

  20. Active part of Charlie--Gibbs fracture zone: A study using sonar and other geophysical techniques

    SciTech Connect

    Searle, R.

    1981-01-10

    A short survey with Gloria side-scan sonar and other geophysical instruments has revealed new information about Charlie--Gibbs fracture zone between 29/sup 0/ and 36 /sup 0/W. The traces of two transform faults have been clearly delineated. They fit small circles about the pole of rotation with an rms error of only about 1 km, but they do not always follow the deepest parts of the transform valleys. The transforms are joined by a short spreading center at 31 /sup 0/45 'W. The median transverse ridge appears to have been produced by normal seafloor spreading at this center and bears identifiable Vine-Matthews magnetic anomalies. A transverse ridge along the eastern inactive part of the northern transform may be an intrusive feature. Considerable thickness of sediment appear to have been deposited in the northern transform valley from Norwegian Sea overflow water passing through the fracture zone, but transverse ridges have prevented the sediment reaching the southern valley.

  1. High-resolution water column survey to identify active sublacustrine hydrothermal discharge zones within Lake Rotomahana, North Island, New Zealand

    NASA Astrophysics Data System (ADS)

    Walker, Sharon L.; de Ronde, Cornel E. J.; Fornari, Daniel; Tivey, Maurice A.; Stucker, Valerie K.

    2016-03-01

    Autonomous underwater vehicles were used to conduct a high-resolution water column survey of Lake Rotomahana using temperature, pH, turbidity, and oxidation-reduction potential (ORP) to identify active hydrothermal discharge zones within the lake. Five areas with active sublacustrine venting were identified: (1) the area of the historic Pink Terraces; (2) adjacent to the western shoreline subaerial "Steaming Cliffs," boiling springs and geyser; (3) along the northern shoreline to the east of the Pink Terrace site; (4) the newly discovered Patiti hydrothermal system along the south margin of the 1886 Tarawera eruption rift zone; and (5) a location in the east basin (northeast of Patiti Island). The Pink Terrace hydrothermal system was active prior to the 1886 eruption of Mount Tarawera, but venting along the western shoreline, in the east basin, and the Patiti hydrothermal system appear to have been initiated in the aftermath of the eruption, similar to Waimangu Valley to the southwest. Different combinations of turbidity, pH anomalies (both positive and negative), and ORP responses suggest vent fluid compositions vary over short distances within the lake. The seasonal period of stratification limits vertical transport of heat to the surface layer and the hypolimnion temperature of Lake Rotomahana consequently increases with an average warming rate of ~ 0.010 °C/day due to both convective hydrothermal discharge and conductive geothermal heating. A sudden temperature increase occurred during our 2011 survey and was likely the response to an earthquake swarm just 11 days prior.

  2. Metallurgical Evaluation of AZ31B-H24 Magnesium Alloy Friction Stir Welds

    NASA Astrophysics Data System (ADS)

    Pareek, M.; Polar, A.; Rumiche, F.; Indacochea, J. E.

    2007-10-01

    Friction Stir welding of 3.175 mm (0.125 in.) thick plates of AZ31-H24 magnesium alloy was performed using several travel velocities and tool-rotation speeds. After production the welds were cross-sectioned and a metallurgical characterization was performed using optical microscopy, and scanning electron microscopy. Assessment of the weld nugget or “stirred zone” shows evidence of dynamic recrystallization and the start of grain growth in some spots of this region compared to the parent metal. Recrystallization was identified in the thermomechanically affected zone (TAZ) as well. The mechanical properties of the weld are correlated with the corresponding microstructures present in the weld nugget and TMAZ. Corrosion resistance of the weld was assessed using Electrochemical Impedance Spectroscopy (EIS) techniques and immersion tests in a corrosive environment; it showed better corrosion resistance than the base metal.

  3. Effect of preheat on TIG welding of AZ61 magnesium alloy

    NASA Astrophysics Data System (ADS)

    Shen, Jun; Xu, Nan

    2012-04-01

    The effects of preheat treatments on the microstructures and mechanical properties of tungsten inert gas (TIG)-welded AZ61 magnesium alloy joints were studied by microstructural observations, microhardness tests and tensile tests. The results showed that the volume fraction of the lamellar β-Mg17(Al,Zn)12 intermetallic compound of in fusion zone (FZ) increased from 15% to 66% with an increase in preheat temperature. Moreover, the microhardness of the FZ and the ultimate tensile strength of the welded joints reached their maximum values when the preheat temperature was 300°C because more lamellar β-Mg17(Al,Zn)12 intermetallic compounds were distributed at the α-Mg grain boundaries and no cracks and pores formed in the FZ of the welded joint.

  4. Secondary Cooling in the Direct-Chill Casting of Magnesium Alloy AZ31

    NASA Astrophysics Data System (ADS)

    Caron, E.; Wells, M. A.

    2009-08-01

    Secondary cooling information is critical when modeling the direct-chill (DC) casting process of magnesium alloys. However, accurate data for the heat flux in the secondary cooling zone are scarce, and most reported research is concerned with the DC casting of aluminum alloys. Cooling experiments that simulated the secondary cooling of magnesium AZ31 were conducted in order to observe the influence of various parameters on the different boiling-water heat-transfer phenomena. The heat flux in each boiling regime was quantified as a function of the cooling-water flow rate, water temperature, and initial sample temperature. Equations developed from the cooling experiments could be combined to build “idealized” boiling curves for a given set of DC casting conditions.

  5. Grain Refinement of AZ31 Magnesium Alloy Weldments by AC Pulsing Technique

    NASA Astrophysics Data System (ADS)

    Kishore Babu, N.; Cross, C. E.

    2012-11-01

    The current study has investigated the influence of alternating current pulsing on the structure and mechanical properties of AZ31 magnesium alloy gas tungsten arc (GTA) weldments. Autogenous full penetration bead-on-plate GTA welds were made under a variety of conditions including variable polarity (VP), variable polarity mixed (VPM), alternating current (AC), and alternating current pulsing (ACPC). AC pulsing resulted in significant refinement of weld metal when compared with the unpulsed conditions. AC pulsing leads to relatively finer and more equiaxed grain structure in GTA welds. In contrast, VP, VPM, and AC welding resulted in predominantly columnar grain structures. The reason for this grain refinement may be attributed to the periodic variations in temperature gradient and solidification rate associated with pulsing as well as weld pool oscillation observed in the ACPC welds. The observed grain refinement was shown to result in an appreciable increase in fusion zone hardness, tensile strength, and ductility.

  6. Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum)

    PubMed Central

    Tsuchiya, Mutsumi; Satoh, Shinobu; Iwai, Hiroaki

    2015-01-01

    After fruit development is triggered by pollination, the abscission zone (AZ) in the fruit pedicel strengthens its adhesion to keep the fruit attached. We previously reported that xyloglucan and arabinan accumulation in the AZ accompanies the shedding of unpollinated flowers. After the fruit has developed and is fully ripened, shedding occurs easily in the AZ due to lignin accumulation. Regulation of cell wall metabolism may play an important role in these processes, but it is not well understood. In the present report, we used immunohistochemistry to visualize changes in the distributions of xyloglucan and arabinan metabolism-related enzymes in the AZs of pollinated and unpollinated flowers, and in ripened fruits. During floral abscission, we observed a gradual increase in polyclonal antibody labeling of expansin in the AZ. The intensities of LM6 and LM15 labeling of arabinan and xyloglucan, respectively, also increased. However, during floral abscission, we observed a large 1 day post anthesis (DPA) peak in the polyclonal antibody labeling of XTH in the AZ, which then decreased. These results suggest that expansin and XTH play important, but different roles in the floral abscission process. During fruit abscission, unlike during floral abscission, no AZ-specific expansin and XTH were observed. Although lignification was seen in the AZ of over-ripe fruit pedicels, secondary cell wall-specific cellulose synthase signals were not observed. This suggests that cellulose metabolism-related enzymes do not play important roles in the AZ prior to fruit abscission. PMID:26029225

  7. Distribution of flares on the sun during 1955-1985 - 'Hot spots' (active zones) lasting for 30 years

    NASA Technical Reports Server (NTRS)

    Bai, Taeil

    1988-01-01

    The coordinates of 'major solar flares' observed during the period from January 1955 through August 1985 are analyzed. About 100 'superactive' regions (large, complex, active regions containing large sunspots) produced 46 percent of the major flares during the period. Superactive regions appeared more frequently in certain areas of the sun called 'hot spots' or 'active zones'. The synodic rotation periods of the northern and southern hemisphere hot spots were 26.72 d and 26.61 d, respectively. One of the two hot spots persisted through three solar cycles, and the other was active during cycles 19 and 21 but was dormant during cycle 20. These findings suggest that the mechanism producing hot spots must be stable for two or three solar cycles or longer.

  8. Incipient Crustal Stretching across AN Active Collision Belt: the Case of the Siculo-Calabrian Rift Zone (central Mediterranean)

    NASA Astrophysics Data System (ADS)

    Catalano, S.; Tortorici, G.; Romagnoli, G.; Pavano, F.

    2012-12-01

    In the Central Mediterranean, the differential roll-back of the subducting Nubia Plate caused the Neogene-Quaternary extrusion of the Calabrian arc onto the oceanic Ionian slab, and the opening of the oceanic Tyrrhenian Basin, in the overriding Eurasia Plate. The differential motion at the edges of the arc was largely accommodated along transform faults that propagated across the orogenic belt. Since the Late Quaternary, the southern edge of the arc has been replaced by the roughly N-S oriented Siculo-Calabrian Rift Zone (SCRZ) that formed as the NNW-directed normal faults of NE Sicily, crossing the orogenic belt, have linked the NNE-oriented Tyrrhenian margin of southern Calabria with the NNW-trending Africa-Ionian boundary of southeastern Sicily. Our study focused on the Sicily shoulder of the SCRZ, where the transition zone between the extensional belt and the still active Nubia-Eurasia convergent margin is characterized by two distinct mobile crustal wedges, both lying on an upwarped Mantle, where a re-orientations of the σ1 is combined with volcanism (e.g. Etna, Aeolian islands) and a huge tectonic uplift. In southeastern Sicily, the Hyblean-Etnean region evolved, since about 0.85 Ma, as an indipendent crustal wedge, moving towards the NNW and pointing to the active Mt. Etna volcano. A local ENE crustal stretching accompanied the traslation of the block and pre-dated the ESE-oriented extension governing the propagation of the southernmost branch of the SCR, which started at about 330 ka B.P.. Similarly, the Peloritani-Aeolian region, flanked by the 125 ka-old NE-Sicily branch of the rift zone, represents a mostly submerged crustal wedge that migrates towards the NE, diverging from the rest of the Sicily collision zone and pointing to the Stromboli volcano. The Peloritani-Aeolian block is characterized by the occurrence of a wide central NE-oriented collapsed basin contoured by an actively uplifting region, whose tectonic boundaries are evidenced by a sharp

  9. Calcium channels link the muscle-derived synapse organizer laminin β2 to Bassoon and CAST/Erc2 to organize presynaptic active zones

    PubMed Central

    Chen, Jie; Billings, Sara E.; Nishimune, Hiroshi

    2013-01-01

    Synapse formation requires the organization of presynaptic active zones, the synaptic vesicle release sites, in precise apposition to postsynaptic neurotransmitter receptor clusters; however, the molecular mechanisms responsible for these processes remain unclear. Here, we show that P/Q-type and N-type voltage-dependent calcium channels (VDCCs) play essential roles as scaffolding proteins in the organization of presynaptic active zones. The neuromuscular junction of double knockout mice for P/Q- and N-type VDCCs displayed a normal size, but had significantly reduced numbers of active zones and docked vesicles and featured an attenuation of the active zone proteins Bassoon, Piccolo, and CAST/Erc2. Consistent with this phenotype, direct interactions of the VDCC β1b or β4 subunits and the active zone-specific proteins Bassoon or CAST/Erc2 were confirmed by immunoprecipitation. A decrease in the number of active zones caused by a loss of presynaptic VDCCs resembled the pathological conditions observed in the autoimmune neuromuscular disorder Lambert–Eaton myasthenic syndrome (LEMS). At the synaptic cleft of double knockout mice, we also observed a decrease of the synaptic organizer laminin β2 protein, an extracellular ligand of P/Q- and N-type VDCCs. However, the transcription level of laminin β2 did not decrease in double knockout mice, suggesting that the synaptic accumulation of laminin β2 protein required its interaction with presynaptic VDCCs. These results suggest that presynaptic VDCCs link the target-derived synapse organizer laminin β2 to active zone proteins and function as scaffolding proteins to anchor active zone proteins to the presynaptic membrane. PMID:21228161

  10. Ground Motion Polarization in the Damage Zone of the Active, Strike-Slip Mattinata Fault, Southern Italy

    NASA Astrophysics Data System (ADS)

    Pischiutta, M.; Cianfarra, P.; Anselmi, M.; Salvini, F.; Rovelli, A.

    2013-12-01

    We have recently observed the occurrence of directional amplification effects in fault zones using both earthquakes and ambient noise records. In several faults we have found that ground motion polarization tends to have a high angle to cleavages produced by the stress related to the kinematics in the fault damage zone. We thus interpret this effect as due to the higher compliance of the fractured rocks of the damage zone in a direction transversal to the cleavage strike. Here we have tested the technique of the wavefield polarization using ambient vibrations recorded across the seismically active Mattinata Fault, in the Gargano Promontory, Italy. This fault has been chosen for the high number of structural investigations led out so far. The Mattinata Fault outcrops for over 40 km and shows an ondulated trajectory that is characterized by a number of significant tectonic-related morphological features compatible with a general left-lateral strike-slip kinematics. These features include a pull-apart basin and a transpressional zone. The main associated cleavage consists of a marked array of disjunctive, spaced pressure-solution surfaces developed within the 200-300 m wide fault damage zone. In order to relate the orientation of cleavage to the ground motion polarization, we measured 20-50 min of ambient noise at about 30 sites chosen in the fault damage zone close to rock outcrops where also structural geological measurements were carried out. Ground motion polarization is assessed both in the frequency and time domain through the individual-station horizontal-to-vertical spectral ratio and covariance-matrix analysis, respectively. Two ambient noise measurements were performed close to permanent broadband stations of the Italian Seismic Telemetric Network. Results are consistent with those inferred on earthquake records at the two permanent stations, confirming that ambient noise yields results consistent with earthquake records as previously observed in other

  11. Evaluation of 241-AZ tank farm supporting phase 1 privatization waste feed delivery

    SciTech Connect

    CARLSON, A.B.

    1998-11-19

    This evaluation is one in a series of evaluations determining the process needs and assessing the adequacy of existing and planned equipment in meeting those needs at various double-shell tank farms in support of Phase 1 privatization. A number of tank-to-tank transfers and waste preparation activities are needed to process and feed waste to the private contractor in support of Phase 1 privatization. The scope of this evaluation is limited to process needs associated with 241-AZ tank farm during the Phase 1 privatization.

  12. 78 FR 41911 - Foreign-Trade Zone 161-Sedgwick County, Kansas; Authorization of Production Activity; Siemens...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-12

    ... Activity; Siemens Energy, Inc. (Wind Turbine Nacelles and Hubs); Hutchinson, Kansas On March 7, 2013... (78 FR 20888, April 8, 2013). The FTZ Board has determined that no further review of the activity...

  13. 78 FR 30862 - Foreign-Trade Zone 41-Milwaukee, Wisconsin; Authorization of Production Activity; CNH America...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ... comment (78 FR 7394, 02-01-2013). The FTZ Board has determined that no further review of the activity is... Activity; CNH America, LLC; Subzone 41I (Tractors and Tractor/Combine Components); Racine, Wisconsin...

  14. Photogrammetry surveys and mosaic: a useful tool to monitor active zones. Applications to the Indonesian Lusi eruption site.

    NASA Astrophysics Data System (ADS)

    Romeo, Giovanni; Di Stefano, Giuseppe; Mazzini, Adriano; Iarocci, Alessandro; Caramelli, Antonio

    2016-04-01

    Unmanned and remotely operated aircraft showed to be an efficient and cost effective way to explore remote or extreme environments. Comparative photogrammetry studies are an efficient way to study and monitor he evolution of geologically active areas and ongoing events and are able to highlight details that are typically lost during traditional field campaigns. The Lusi mud eruption in eastern Java (Indonesia) represents one of the most spectacular geological phenomena that is ongoing since May 2006. In the framework of the Lusi Lab project (ERC grant n° 308126) we designed and constructed a multipurpose drone to survey the eruption site. Among the numerous other payloads, the Lusi drone is equipped with Olympus EPM-2 and Go-Pro Hero3 cameras that allow the operator to collect video stills, high quality pictures and to complete photogrammetry surveys. Targeted areas have been selected for detailed studies in the 7 km2 region inside the embankment that was prevent the mud burial of the settlements in the Sidoarjo Regency. The region is characterized by the presence of the Watukosek fault zone. This strike slip system originates from the Arjuno-Welirang volcanic complex and extends to the north east of the Java Island intersecting the Lusi crater. Therefore of particular interest are the faulted surveyed areas present around the Lusi crater inside the embankment. Results reveal a surprising accuracy for the collected mosaic. Multiple surveys are able to reveal the changes and the evolution of the fault through time and to indicate more active zones. In particular this type of survey can highlight the weakness zones and is thus useful to prevent potential geohazards in the area. The poster shows the aerial survey results, including a 3d-printed slice of LuSi, obtained combining 2500 16 Mp photographs. A 3d zoomed detail is also shown, evidencing the resolution that this technique can offer.

  15. Traumatic Brain Injury Activation of the Adult Subventricular Zone Neurogenic Niche.

    PubMed

    Chang, Eun Hyuk; Adorjan, Istvan; Mundim, Mayara V; Sun, Bin; Dizon, Maria L V; Szele, Francis G

    2016-01-01

    Traumatic brain injury (TBI) is common in both civilian and military life, placing a large burden on survivors and society. However, with the recognition of neural stem cells in adult mammals, including humans, came the possibility to harness these cells for repair of damaged brain, whereas previously this was thought to be impossible. In this review, we focus on the rodent adult subventricular zone (SVZ), an important neurogenic niche within the mature brain in which neural stem cells continue to reside. We review how the SVZ is perturbed following various animal TBI models with regards to cell proliferation, emigration, survival, and differentiation, and we review specific molecules involved in these processes. Together, this information suggests next steps in attempting to translate knowledge from TBI animal models into human therapies for TBI. PMID:27531972

  16. Traumatic Brain Injury Activation of the Adult Subventricular Zone Neurogenic Niche

    PubMed Central

    Chang, Eun Hyuk; Adorjan, Istvan; Mundim, Mayara V.; Sun, Bin; Dizon, Maria L. V.; Szele, Francis G.

    2016-01-01

    Traumatic brain injury (TBI) is common in both civilian and military life, placing a large burden on survivors and society. However, with the recognition of neural stem cells in adult mammals, including humans, came the possibility to harness these cells for repair of damaged brain, whereas previously this was thought to be impossible. In this review, we focus on the rodent adult subventricular zone (SVZ), an important neurogenic niche within the mature brain in which neural stem cells continue to reside. We review how the SVZ is perturbed following various animal TBI models with regards to cell proliferation, emigration, survival, and differentiation, and we review specific molecules involved in these processes. Together, this information suggests next steps in attempting to translate knowledge from TBI animal models into human therapies for TBI. PMID:27531972

  17. Maximal radius of the aftershock zone in earthquake networks

    NASA Astrophysics Data System (ADS)

    Mezentsev, A. Yu.; Hayakawa, M.

    2009-09-01

    In this paper, several seismoactive regions were investigated (Japan, Southern California and two tectonically distinct Japanese subregions) and structural seismic constants were estimated for each region. Using the method for seismic clustering detection proposed by Baiesi and Paczuski [M. Baiesi, M. Paczuski, Phys. Rev. E 69 (2004) 066106; M. Baiesi, M. Paczuski, Nonlin. Proc. Geophys. (2005) 1607-7946], we obtained the equation of the aftershock zone (AZ). It was shown that the consideration of a finite velocity of seismic signal leads to the natural appearance of maximal possible radius of the AZ. We obtained the equation of maximal radius of the AZ as a function of the magnitude of the main event and estimated its values for each region.

  18. Trace Hydrophobic Organic Chemicals Present in Pennsylvania Groundwater are Correlated with Geogenic Brines rather than Hydraulic Fracturing Active Zones

    NASA Astrophysics Data System (ADS)

    Drollette, B.; Shregglman, K.; D'Ambro, E.; Elsner, M.; Warner, N. R.; O'Connor, M.; Karatum, O.; Vengosh, A.; Jackson, R. B.; Darrah, T.; Plata, D.

    2014-12-01

    Recent studies demonstrated that deep Marcellus shale brines migrate into shallow groundwater aquifers, presumably via fractures in the subsurface that exist independent of any gas extraction activities. However, whereas many inorganic species are conservative tracers, hydrophobic organic compounds are both sorptive and reactive, and geogenic organic chemicals may not survive transport from deep shales to the subsurface. Here, 40 shallow groundwater samples from private wells in Northeastern Pennsylvania were analyzed for volatile organic compounds (VOCs) and gasoline range organic compounds (GRO), and 17 were analyzed for VOCs, GRO, and diesel range organic compounds (DRO). BTEX compounds (i.e., benzene, toluene, ethylbenzene, and xylenes) were detected in 6 of 40 samples at concentrations orders of magnitude below EPA maximum contaminant levels (e.g., << 5 ppb) and did not spatially correlate with distance to the nearest active hydraulic fracturing well. GRO was detected in 10 of 40 samples at concentrations as high as 8.8 ± 0.4 ppb and did not correlate with distance to the nearest hydraulic fracturing well (p = 0.24) nor in active fracturing zones, which we defined as sample locations less than 1 km from a well (p = 0.60). However, GRO was strongly correlated (p = 0.004) with shallow groundwater with Marcellus Shale inorganic chemical character, as delineated by inorganic chemical analysis. DRO was detected in all 17 samples up to 158 ± 4 ppb and did not spatially correlate with distance to the nearest hydraulic fracturing well (p = 0.74), nor active zones (p = 0.61). Similar to GRO, DRO did correlate with shallow groundwater containing Marcellus Shale character with moderate significance (p = 0.08). These results indicate that: (a) hydrophobic organic chemicals can survive transport from the deep subsurface to shallow groundwaters, and (b) transport of these compounds is not detectably enhanced by hydraulic fracturing activities in Northeastern PA as of the

  19. Isotopic evidence (B, C, O) of deep fluid processes in fault rocks from the active Woodlark Basin detachment zone

    NASA Astrophysics Data System (ADS)

    Kopf, Achim; Behrmann, Jan H.; Deyhle, Annette; Roller, Sybille; Erlenkeuser, Helmut

    2003-03-01

    We report results from boron, carbon and oxygen stable isotope analyses of faulted and veined rocks recovered by scientific ocean drilling during ODP Leg 180 in the western Woodlark Basin, off Papua New Guinea. In this area of active continental extension, crustal break-up and incipient seafloor spreading, a shallow-dipping, seismically active detachment fault accommodates strain, defining a zone of mylonites and cataclasites, vein formation and fluid infiltration. Syntectonic microstructures and vein-fill mineralogy suggest frictional heating during slip during extension and exhumation of Moresby Seamount. Low carbon and oxygen isotope ratios of calcite veins indicate precipitation from hydrothermal fluids (δ 13C PDB down to -17‰; δ 18O PDB down to -22‰) formed by both dehydration and decarbonation. Boron contents are low (<7 ppm), indicating high-grade metamorphic source rock for the fluids. Some of the δ 11B signatures (17-35‰; parent solutions to calcite vein fills) are low when compared to deep-seated waters in other tectonic environments, likely reflecting preferential loss of 11B during low-grade metamorphism at depth. Pervasive devolatilization and flux of CO 2-rich fluids are evident from similar vein cement geochemistry in the detachment fault zone and splays further updip. Multiple rupture-and-healing history of the veins suggests that precipitation may be an important player in fluid pressure evolution and, hence, seismogenic fault movement.

  20. A Drosophila model of GSS syndrome suggests defects in active zones are responsible for pathogenesis of GSS syndrome

    PubMed Central

    Choi, Jin-Kyu; Jeon, Yong-Chul; Lee, Dae-Weon; Oh, Jae-Min; Lee, Hyun-Pil; Jeong, Byung-Hoon; Carp, Richard I.; Koh, Young Ho; Kim, Yong-Sun

    2010-01-01

    We have established a Drosophila model of Gerstmann–Sträussler–Scheinker (GSS) syndrome by expressing mouse prion protein (PrP) having leucine substitution at residue 101 (MoPrPP101L). Flies expressing MoPrPP101L, but not wild-type MoPrP (MoPrP3F4), showed severe defects in climbing ability and early death. Expressed MoPrPP101L in Drosophila was differentially glycosylated, localized at the synaptic terminals and mainly present as deposits in adult brains. We found that behavioral defects and early death of MoPrPP101L flies were not due to Caspase 3-dependent programmed cell death signaling. In addition, we found that Type 1 glutamatergic synaptic boutons in larval neuromuscular junctions of MoPrPP101L flies showed significantly increased numbers of satellite synaptic boutons. Furthermore, the amount of Bruchpilot and Discs large in MoPrPP101L flies was significantly reduced. Brains from scrapie-infected mice showed significantly decreased ELKS, an active zone matrix marker compared with those of age-matched control mice. Thus, altered active zone structures at the molecular level may be involved in the pathogenesis of GSS syndrome in Drosophila and scrapie-infected mice. PMID:20829230

  1. Fracturing and earthquake activity within the Prestahnúkur fissure swarm in the Western Volcanic Rift Zone of Iceland

    NASA Astrophysics Data System (ADS)

    Hjartardóttir, Ásta Rut; Hjaltadóttir, Sigurlaug; Einarsson, Páll; Vogfjörd, Kristín.; Muñoz-Cobo Belart, Joaquín.

    2015-12-01

    The Prestahnúkur fissure swarm is located within the ultraslowly spreading Western Volcanic Zone in Iceland. The fissure swarm is characterized by normal faults, open fractures, and evidence of subglacial fissure eruptions (tindars). In this study, fractures and faults within the Prestahnúkur fissure swarm were mapped in detail from aerial photographs to determine the extent and activity of the fissure swarm. Earthquakes during the last ~23 years were relocated to map the subsurface fault planes that they delineate. The Prestahnúkur fissure swarm is 40-80 km long and up to ~20 km wide. Most of the areas of the fissure swarm have been glacially eroded, although a part of it is covered by postglacial lava flows. The fissure swarm includes numerous faults with tens of meters vertical offset within the older glacially eroded part, whereas open fractures are found within postglacial lava flows. Comparison of relocated earthquakes and surface fractures indicates that some of the surface fractures have been activated at depth during the last ~23 years, although no dike intrusions have been ongoing. The existence of tindars nevertheless indicates that dike intrusions and rifting events do occur within the Prestahnúkur fissure swarm. The low-fracture density within postglacial lava flows and low density of postglacial eruptive fissures indicate that rifting episodes occur less often than in the faster spreading Northern Volcanic Zone.

  2. Offshore active faults of the Mikata fault zone in Fukui, Japan, revealed by high-resolution seismic profiles

    NASA Astrophysics Data System (ADS)

    Inoue, T.; Sugiyama, Y.; Sakamoto, I.; Takino, Y.; Murakami, F.; Hosoya, T.; Usami, T.

    2014-12-01

    The Mikata fault zone are located in coastal and shallow sea area off Fukui Prefecture, West Japan. National Institute of Advanced Industrial Science and Technology (AIST) and Tokai University conducted, as part of MEXT 2013 nearshore active fault survey project, a high-resolution multi-channel seismic survey using Boomer and a 12-channel streamer cable, acoustic profiling survey using parametric sub-bottom profiler and shallow-sea offshore drilling, in order to clarify distribution and activity of the Mikata fault zone. The seismic reflection surveys identified four reflection surfaces as vertical displacement markers in the post-glacial deposits at a depth ranging from ca. 4.5m to ca. 17m below the sea bottom on the downthrown side. We estimated the age of each marker reflection surface by using the C14 age and others from 4m-long core obtained on the downthrown side of fault and the sea level change in the latest Pleistocene and early Holocene around Japan. The results of these surveys have revealed that the fault system was reactivated three times since the latest Pleistocene. The vertical slip rate and average recurrence interval of the fault system are estimated at ca. 0.8-1.0 m/ky and 2,000-3,800 years, respectively.

  3. A structured three-dimensional polymer electrolyte with enlarged active reaction zone for Li-O2 batteries

    NASA Astrophysics Data System (ADS)

    Bonnet-Mercier, Nadège; Wong, Raymond A.; Thomas, Morgan L.; Dutta, Arghya; Yamanaka, Keisuke; Yogi, Chihiro; Ohta, Toshiaki; Byon, Hye Ryung

    2014-11-01

    The application of conventional solid polymer electrolyte (SPE) to lithium-oxygen (Li-O2) batteries has suffered from a limited active reaction zone due to thick SPE and subsequent lack of O2 gas diffusion route in the positive electrode. Here we present a new design for a three-dimensional (3-D) SPE structure, incorporating a carbon nanotube (CNT) electrode, adapted for a gas-based energy storage system. The void spaces in the porous CNT/SPE film allow an increased depth of diffusion of O2 gas, providing an enlarged active reaction zone where Li+ ions, O2 gas, and electrons can interact. Furthermore, the thin SPE layer along the CNT, forming the core/shell nanostructure, aids in the smooth electron transfer when O2 gas approaches the CNT surface. Therefore, the 3-D CNT/SPE electrode structure enhances the capacity in the SPE-based Li-O2 cell. However, intrinsic instability of poly(ethylene oxide) (PEO) of the SPE matrix to superoxide (O2.-) and high voltage gives rise to severe side reactions, convincing us of the need for development of a more stable electrolyte for use in this CNT/SPE design.

  4. A structured three-dimensional polymer electrolyte with enlarged active reaction zone for Li–O2 batteries

    PubMed Central

    Bonnet-Mercier, Nadège; Wong, Raymond A.; Thomas, Morgan L.; Dutta, Arghya; Yamanaka, Keisuke; Yogi, Chihiro; Ohta, Toshiaki; Byon, Hye Ryung

    2014-01-01

    The application of conventional solid polymer electrolyte (SPE) to lithium-oxygen (Li–O2) batteries has suffered from a limited active reaction zone due to thick SPE and subsequent lack of O2 gas diffusion route in the positive electrode. Here we present a new design for a three-dimensional (3-D) SPE structure, incorporating a carbon nanotube (CNT) electrode, adapted for a gas-based energy storage system. The void spaces in the porous CNT/SPE film allow an increased depth of diffusion of O2 gas, providing an enlarged active reaction zone where Li+ ions, O2 gas, and electrons can interact. Furthermore, the thin SPE layer along the CNT, forming the core/shell nanostructure, aids in the smooth electron transfer when O2 gas approaches the CNT surface. Therefore, the 3-D CNT/SPE electrode structure enhances the capacity in the SPE-based Li–O2 cell. However, intrinsic instability of poly(ethylene oxide) (PEO) of the SPE matrix to superoxide (O2·−) and high voltage gives rise to severe side reactions, convincing us of the need for development of a more stable electrolyte for use in this CNT/SPE design. PMID:25410536

  5. Mixer pump test plan for double shell tank AZ-101

    SciTech Connect

    STAEHR, T.W.

    1999-05-12

    Mixer pump systems have been chosen as the method for retrieval of tank wastes contained in double shell tanks at Hanford. This document describes the plan for testing and demonstrating the ability of two 300 hp mixer pumps to mobilize waste in tank AZ-101. The mixer pumps, equipment and instrumentation to monitor the test were installed by Project W-151.

  6. 78 FR 3877 - Radio Broadcasting Services; Peach Springs, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-17

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Peach Springs, AZ AGENCY: Federal Communications... Springs, Arizona, as a Tribal Allotment. Channel 265A would constitute a first tribal allotment and a second potential service at Peach Springs. Channel 265A can be allotted at Peach Springs, Arizona,...

  7. 75 FR 76293 - Radio Broadcasting Services; Peach Springs, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Peach Springs, AZ AGENCY: Federal Communications..., allots FM Channel 281C3 at Peach Springs, Arizona, in order to maintain a first local service at that community. Channel 281C3 can be allotted at Peach Springs, Arizona, in compliance with the...

  8. 75 FR 57327 - Environmental Impact Statement; Pinal County, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-20

    ... Federal Highway Administration Environmental Impact Statement; Pinal County, AZ AGENCY: Federal Highway... public that an Environmental impact Statement will be prepared for a proposed transportation project in... prepare an environmental impact statement (EIS) on a proposed 40-mile-long project along a new...

  9. 75 FR 883 - Environmental Impact Statement; Maricopa County, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-06

    ... Federal Highway Administration Environmental Impact Statement; Maricopa County, AZ AGENCY: Federal Highway... public that an Environmental Impact Statement will be prepared for a proposed highway project in Maricopa... prepare an Environmental Impact Statement (EIS) on proposed operational improvements to the Interstate...

  10. 77 FR 10649 - Modification of Class E Airspace; Douglas, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-23

    ... Administration 14 CFR Part 71 Modification of Class E Airspace; Douglas, AZ AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action modifies Class E airspace at Bisbee Douglas... typographical error in the legal description for the Class E 700 foot airspace. This improves the safety...

  11. 78 FR 17743 - Navajo Nation Disaster #AZ-00026

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-22

    ... ADMINISTRATION Navajo Nation Disaster AZ-00026 AGENCY: U.S. Small Business Administration. ACTION: Notice... for the Navajo Nation (FEMA-4104- DR), dated 03/05/2013. Incident: Severe Freeze. Incident Period: 12...: Primary Areas: Navajo Nation and Associated Lands. The Interest Rates are: For Physical Damage:...

  12. 76 FR 18378 - Amendment of Class E Airspace; Taylor, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-04

    ... amend controlled airspace at Taylor, AZ (76 FR 3570). Interested parties were invited to participate in... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of... U.S.C. 106(g), 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec....

  13. 76 FR 45177 - Establishment of Class E Airspace; Kayenta, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... Navigation (RNAV) Global Positioning System (GPS) standard instrument approach procedures at Kayenta Airport... Federal Register a notice of proposed rulemaking to establish controlled airspace at Kayenta, AZ (76 FR... a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26,...

  14. Identifying active interplate and intraplate fault zones in the western Caribbean plate from seismic reflection data and the significance of the Pedro Bank fault zone in the tectonic history of the Nicaraguan Rise

    NASA Astrophysics Data System (ADS)

    Ott, B.; Mann, P.

    2015-12-01

    The offshore Nicaraguan Rise in the western Caribbean Sea is an approximately 500,000 km2 area of Precambrian to Late Cretaceous tectonic terranes that have been assembled during the Late Cretaceous formation of the Caribbean plate and include: 1) the Chortis block, a continental fragment; 2) the Great Arc of the Caribbean, a deformed Cretaceous arc, and 3) the Caribbean large igneous province formed in late Cretaceous time. Middle Eocene to Recent eastward motion of the Caribbean plate has been largely controlled by strike-slip faulting along the northern Caribbean plate boundary zone that bounds the northern margin of the Nicaraguan Rise. These faults reactivate older rift structures near the island of Jamaica and form the transtensional basins of the Honduran Borderlands near Honduras. Recent GPS studies suggest that small amount of intraplate motion within the current margin of error of GPS measurements (1-3 mm/yr) may occur within the center of the western Caribbean plate at the Pedro Bank fault zone and Hess Escarpment. This study uses a database of over 54,000 km of modern and vintage 2D seismic data, combined with earthquake data and results from previous GPS studies to define the active areas of inter- and intraplate fault zones in the western Caribbean. Intraplate deformation occurs along the 700-km-long Pedro Bank fault zone that traverses the center of the Nicaraguan Rise and reactivates the paleo suture zone between the Great Arc of the Caribbean and the Caribbean large igneous province. The Pedro Bank fault zone also drives active extension at the 200-km-long San Andres rift along the southwest margin of the Nicaraguan Rise. Influence of the Cocos Ridge indentor may be contributing to reactivation of faulting along the southwesternmost, active segment of the Hess Escarpment.

  15. 78 FR 9667 - Foreign-Trade Zone 50-Long Beach, California; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-11

    ... Production Activity; Panasonic Corporation of North America (Kitting of Consumer Electronics); Anaheim, CA... FTZ 50. The facility is used for the kitting of consumer electronics parts into retail...

  16. 78 FR 24158 - Foreign-Trade Zone (FTZ) 75-Phoenix, Arizona; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... Production Activity; Orbital Sciences Corporation (Satellites and Spacecraft Launch Vehicles); Gilbert... satellites and spacecraft launch vehicles. Pursuant to 15 CFR 400.14(b), FTZ activity would be limited to the... procedures that applies to satellites and spacecraft launch vehicles (free) for the foreign status...

  17. 78 FR 49254 - Foreign-Trade Zone 158-Vicksburg/Jackson, Mississippi; Authorization of Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-13

    ... comment (78 FR 20889-20890, 4-8-2013). The FTZ Board has determined that no further review of the activity... Production Activity; Extension of Production Authority; H.M. Richards, Inc. (Upholstered Furniture); Guntown... H.M. Richards, Inc., in Guntown, Mississippi. The notification was processed in accordance with...

  18. 78 FR 28190 - Foreign-Trade Zone (FTZ) 134-Chattanooga, Tennessee; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-14

    ... Production Activity; Komatsu America Corporation (Construction and Forestry Equipment Production... of proposed production activity to the FTZ Board on behalf of Komatsu America Corporation (Komatsu... located within Site 14 of FTZ 134 (S-38- 2013, 4-2-2013). The facility is used for the production...

  19. 78 FR 42930 - Foreign-Trade Zone (FTZ) 114-Peoria, Illinois; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-18

    ... Production Activity, Easton-Bell Sports, Inc. (Sports Equipment), Rantoul, Illinois Easton-Bell Sports, Inc. (Easton-Bell Sports) submitted a notification of proposed production activity to the FTZ Board for its... status at the Easton-Bell Sports facility was submitted and is being processed under Section 400.31...

  20. 78 FR 39707 - Foreign-Trade Zone (FTZ) 32-Miami, Florida; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-02

    ... Activity; Brightstar Corporation; (Cell Phone Kitting); Miami, Florida The Greater Miami Chamber of... cell phones and cell phone accessories. Pursuant to 15 CFR 400.14(b), FTZ activity would be limited to... during customs entry procedures that apply to cell phones (duty rate 0%) for the foreign status...

  1. Online Asynchronous Threaded Discussions: Good Enough to Advance Students through the Proximal Zone of Activity Theory?

    ERIC Educational Resources Information Center

    Maurino, Paula San Millan

    2007-01-01

    This paper presents a study which involved researching student interaction and participation under the lens of Activity Theory and Social Computing. Activity Theory is a philosophical framework that integrates the objective, the sociocultural, and the ecological, while Social Computing describes any type of computing application in which software…

  2. Structure of the active rift zone and margins of the northern Imperial Valley from Salton Seismic Imaging Project (SSIP) data

    NASA Astrophysics Data System (ADS)

    Livers, A.; Han, L.; Delph, J. R.; White-Gaynor, A. L.; Petit, R.; Hole, J. A.; Stock, J. M.; Fuis, G. S.

    2012-12-01

    First-arrival refraction data were used to create a seismic velocity model of the upper crust across the actively rifting northern Imperial Valley and its margins. The densely sampled seismic refraction data were acquired by the Salton Seismic Imaging Project (SSIP) , which is investigating rift processes in the northern-most rift segment of the Gulf of California extensional province and earthquake hazards at the southern end of the San Andreas Fault system. A 95-km long seismic line was acquired across the northern Imperial Valley, through the Salton Sea geothermal field, parallel to the five Salton Butte volcanoes and perpendicular to the Brawley Seismic Zone and major strike-slip faults. Nineteen explosive shots were recorded with 100 m seismometer spacing across the valley and with 300-500 m spacing into the adjacent ranges. First-arrival travel times were picked from shot gathers along this line and a seismic velocity model was produced using tomographic inversion. Sedimentary basement and seismic basement in the valley are interpreted to be sediment metamorphosed by the very high heat flow. The velocity model shows that this basement to the west of the Brawley Seismic Zone is at ~4-km depth. The basement shallows to ~2-km depth in the active geothermal field and Salton Buttes volcanic field which locally coincide with the Brawley Seismic Zone. At the eastern edge of the geothermal field, the basement drops off again to ~3.5-km depth. The eastern edge of the valley appears to be fault bounded by the along-strike extension of the Sand Hills Fault, an inactive strike-slip fault. The seismic velocities to the east of the fault correspond to metamorphic rock of the Chocolate Mountains, different from the metamorphosed basement in the valley. The western edge of the valley appears to be fault bounded by the active Superstition Hills Fault. To the west of the valley, >4-km deep valley basement extends to the active Superstition Hills Fault. Basement then shallows

  3. RARγ is required for correct deposition and removal of Suz12 and H2A.Z in embryonic stem cells

    PubMed Central

    Amat, Ramon; Gudas, Lorraine J.

    2012-01-01

    Retinoic acid (RA) induces embryonic stem cell differentiation. The effects of RA are mediated by retinoic acid receptors (RARs) that promote epigenetic changes controlling gene transcription. We show here that RARγ, in the absence of the ligand RA, is required for deposition of the histone variant H2A.Z and the Polycomb protein Suz12 at RA target genes, and that both RARγ and Suz12 exist in a multi-protein complex in the absence of ligand. Addition of RA causes removal of H2A.Z and Suz12 from RARγ target genes when the genes are transcriptionally activated. PMID:20857416

  4. [Agricultural activities and epidemiology of malaria in Soudano-Sahelian zone in Cameroon].

    PubMed

    Atangana, J; Fomena, A; Tamesse, J Lebel; Fondjo, E

    2012-02-01

    We have comparatively studied the dynamics of malaria transmission in the villages of Mokolo-Douvar located in the rural area with traditional agriculture and Gounougou irrigated rice area, in 2004 August and November and 2006 May and October, to assess vectors biting habits, and malaria inoculation rate and malaria parasite prevalence in cohort of children from 0 to 15 years. Mosquitoes were collected by landing catches on volunteers and by pyrethrum spray collections. A total of 5961 Anopheles were collected. Seven Anopheles species were identified: Anopheles gambiae s.s., Anopheles arabiensis, Anopheles funestus, Anopheles pharoensis, Anopheles rufipes, Anopheles ziemanni and Anopheles squamosus. A. arabiensis was the major species (56.2%) and the main malaria vector in both study sites, followed by A. funestus (32.6%). Malaria transmission was high in the irrigated area of Gounougou (1.42 infection bites per man per night) whereas in the non-irrigated zone of Mokolo-Douvar, it was below detection level during the rainy season (0,245 ib/h/n). In Gounougou, a total of 655 children were examined. The mean plasmodic index was 21.1%. Our findings confirm that changes in irrigated rice agriculture influence malaria transmission dynamics, and call for control measures that are readily adapted to local eco-epidemiological settings. PMID:22294407

  5. Indomethacin treatment reduces microglia activation and increases numbers of neuroblasts in the subventricular zone and ischaemic striatum after focal ischaemia.

    PubMed

    Lopes, Rosana S; Cardoso, Marcelo M; Sampaio, Arthur O; Barbosa, Mario Santos; Souza, Celice C; DA Silva, Michelle C; Ferreira, Elane Magno N; Freire, Marco Aurelio M; Lima, Rafael Rodrigues; Gomes-Leal, Walace

    2016-09-01

    Neuroblasts from the subventricular zone (SVZ) migrate to striatum following stroke, but most of them die in the ischaemic milieu and this can be related to exacerbated microglial activation. Here, we explored the effects of the non-steroidal anti-inflammatory indomethacin on microglial activation, neuronal preservation and neuroblast migration following experimental striatal stroke in adult rats. Animals were submitted to endothelin-1 (ET-1)-induced focal striatal ischaemia and were treated with indomethacin or sterile saline (i.p.) for 7 days, being perfused after 8 or 14 days. Immunohistochemistry was performed to assess neuronal loss (anti-NeuN), microglial activation (anti-Iba1, ED1) and migrating neuroblasts (anti-DCX) by counting NeuN, ED1 and DCX-positive cells in the ischaemic striatum or SVZ. Indomethacin treatment reduced microglia activation and the number of ED1+ cells in both 8 and 14 days post injury as compared with controls. There was an increase in the number of DCX+ cells in both SVZ and striatum at the same survival times. Moreover, there was a decrease in the number of NeuN+ cells in indomethacin-treated animals as compared with the control group at 8 days but not after 14 days post injury. Our results suggest that indomethacin treatment modulates microglia activation, contributing to increased neuroblast proliferation in the SVZ and migration to the ischaemic striatum following stroke. PMID:27581930

  6. 78 FR 28801 - Foreign-Trade Zone 22-Chicago, Illinois; Authorization of Production Activity Panasonic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ... comment (78 FR 5773, 1-28-2013). The FTZ Board has determined that no further review of the activity is... Panasonic Corporation of North America (Kitting of Consumer Electronics) Aurora, Illinois On January...

  7. 78 FR 33808 - Foreign-Trade Zone 50-Long Beach, California; Authorization of Production Activity; Panasonic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-05

    ... in the Federal Register inviting public comment (78 FR 9667, 2-11-2013). The FTZ Board has determined... Activity; Panasonic Corporation of North America (Kitting of Consumer Electronics); Anaheim, California...

  8. 78 FR 55057 - Foreign-Trade Zone 134-Chattanooga, Tennessee; Authorization of Production Activity; Komatsu...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-09

    ... inviting public comment (78 FR 28190, 05-14-2013). The FTZ Board has determined that no further review of... Activity; Komatsu America Corporation, (Construction and Forestry Equipment), Chattanooga, Tennessee On...

  9. Influence of season on daytime behavioral activities of donkeys in the Northern Guinea Savanna zone of Nigeria

    PubMed Central

    ZAKARI, Friday Ocheja; AYO, Joseph Olusegun; REKWOT, Peter Ibrahim; KAWU, Mohammed Umar

    2016-01-01

    ABSTRACT The present experiment was performed with the aim of investigating the effect of season on behavioral activities of donkeys during the rainy and harmattan seasons in the Northern Guinea zone of Nigeria. Sixteen apparently healthy donkeys were used as subjects and divided into four groups based on age. During each season, behavioral activities of each donkey were evaluated for three weeks using the focal animal sampling technique. The dry-bulb temperature (DBT), relative humidity (RH), and temperature-humidity index (THI) were obtained three times each day during the experimental period using standard procedures. In the rainy season, the mean DBT (31.65 ± 0.49°C), RH (73.63 ± 1.09%), and THI (84.39 ± 0.71) were significantly (P<0.0001) higher than the corresponding values of 24.00 ± 0.44°C, 36.80 ± 0.92%, and 64.80 ± 0.62 in the harmattan season. During the rainy season, the donkeys spent 60.00 ± 0.77%, 25.40 ± 0.69%, and 2.94 ± 0.21% on grazing, resting, and grooming, respectively. During the harmattan season, the donkeys spent the most time on grazing (76.76 ± 0.43%), less time on resting (11.97 ± 0.38%), and the least time on grooming (0.89 ± 0.05%). In conclusion, season and seasonal variations affect the daytime behavioral activities of donkeys in the zone, and this should be considered in husbandry practices for donkeys. PMID:26858575

  10. Influence of season on daytime behavioral activities of donkeys in the Northern Guinea Savanna zone of Nigeria.

    PubMed

    Zakari, Friday Ocheja; Ayo, Joseph Olusegun; Rekwot, Peter Ibrahim; Kawu, Mohammed Umar

    2015-01-01

    The present experiment was performed with the aim of investigating the effect of season on behavioral activities of donkeys during the rainy and harmattan seasons in the Northern Guinea zone of Nigeria. Sixteen apparently healthy donkeys were used as subjects and divided into four groups based on age. During each season, behavioral activities of each donkey were evaluated for three weeks using the focal animal sampling technique. The dry-bulb temperature (DBT), relative humidity (RH), and temperature-humidity index (THI) were obtained three times each day during the experimental period using standard procedures. In the rainy season, the mean DBT (31.65 ± 0.49°C), RH (73.63 ± 1.09%), and THI (84.39 ± 0.71) were significantly (P<0.0001) higher than the corresponding values of 24.00 ± 0.44°C, 36.80 ± 0.92%, and 64.80 ± 0.62 in the harmattan season. During the rainy season, the donkeys spent 60.00 ± 0.77%, 25.40 ± 0.69%, and 2.94 ± 0.21% on grazing, resting, and grooming, respectively. During the harmattan season, the donkeys spent the most time on grazing (76.76 ± 0.43%), less time on resting (11.97 ± 0.38%), and the least time on grooming (0.89 ± 0.05%). In conclusion, season and seasonal variations affect the daytime behavioral activities of donkeys in the zone, and this should be considered in husbandry practices for donkeys. PMID:26858575

  11. Three-dimensional electrical resistivity image of magma beneath an active continental rift, Taupo Volcanic Zone, New Zealand

    NASA Astrophysics Data System (ADS)

    Heise, Wiebke; Caldwell, T. Grant; Bibby, Hugh M.; Bennie, Stewart L.

    2010-05-01

    Magmatic activity in regions of continental extension may result in huge (>400 km3) explosive eruptions of viscous, gas-rich silicic-magma. Geochemical and geological data suggest that the large volumes of magma erupted are produced by extracting interstitial liquid from a long-lived ‘mush zone’ (a mixture of solid crystals and liquid melt) that accumulates in liquid-dominated lenses at the top of a much thicker region of lower melt-fraction mush. Such lenses will be highly electrically conductive compared with normal mid-crustal rocks. Here we use results of 220 magnetotelluric (MT) soundings to construct a 3-D electrical resistivity image of the northern (silicic) part of New Zealand's Taupo Volcanic Zone, a young continental rift associated with very high heat flow and intense silicic volcanism. The electrical resistivity image shows a plume-like structure of high conductivity, interpreted to be a zone of interconnected melt, rising from depths >35 km beneath the axis of extension.

  12. Runx1 Activities in Superficial Zone Chondrocytes, Osteoarthritic Chondrocyte Clones and Response to Mechanical Loading

    PubMed Central

    LeBlanc, Kimberly T.; Walcott, Marie E.; Gaur, Tripti; O’Connell, Shannon L.; Basil, Kirti; Tadiri, Christina P.; Mason-Savas, April; Silva, Jason A.; van Wijnen, Andre J.; Stein, Janet L.; Stein, Gary S; Ayers, David C.; Lian, Jane B.; Fanning, Paul J.

    2015-01-01

    Objective Runx1, the hematopoietic lineage determining transcription factor, is present in perichondrium and chondrocytes. Here we addressed Runx1 functions, by examining expression in cartilage during mouse and human osteoarthritis (OA) progression and in response to mechanical loading. Methods Spared and diseased compartments in knees of OA patients and in mice with surgical destabilization of the medial meniscus were examined for changes in expression of Runx1 mRNA (Q-PCR) and protein (immunoblot, immunohistochemistry). Runx1 levels were quantified in response to static mechanical compression of bovine articular cartilage. Runx1 function was assessed by cell proliferation (Ki67, PCNA) and cell type phenotypic markers. Results Runx1 is enriched in superficial zone (SZ) chondrocytes of normal bovine, mouse, and human tissues. Increasing loading conditions in bovine cartilage revealed a positive correlation with a significant elevation of Runx1. Runx1 becomes highly expressed at the periphery of mouse OA lesions and in human OA chondrocyte ‘clones’ where Runx1 co-localizes with Vcam1, the mesenchymal stem cell (MSC) marker and lubricin (Prg4), a cartilage chondroprotective protein. These OA induced cells represent a proliferative cell population, Runx1 depletion in MPCs decreases cell growth, supporting Runx1 contribution to cell expansion. Conclusion The highest Runx1 levels in SZC of normal cartilage suggest a function that supports the unique phenotype of articular chondrocytes, reflected by upregulation under conditions of compression. We propose Runx1 co-expression with Vcam1 and lubricin in murine cell clusters and human ‘clones’ of OA cartilage, participate in a cooperative mechanism for a compensatory anabolic function. PMID:25078095

  13. The Presence of a Stable Block bounded by Active Zones (Mobile Belts) in the southwestern North American Proterozoic craton

    NASA Astrophysics Data System (ADS)

    Goodell, P.; Martinez P, C.; Mahar, M. A.

    2014-12-01

    Bouguer gravity data, initial Sr isotope values, zircon U-Pb, and multiple occurrences of felsic Proterozoic rocks, have revealed an elevated, less deformed, felsic cratonic block in the northern Mexico. The block is situated in western Chihuahua and is bounded by active zones or mobile belts on three sides, and is here referred to as the Western Chihuahua Cratonic Block (WCCB). Bouguer gravity data clearly indicate a region of a highly negative anomaly (< -200 mgal) in contrast to adjoining areas. The region is large and the anomaly is relatively smooth over broad areas; the WCCB appears as a smaller version of the Colorado Plateau. The block is characterized by high initial Sr isotope ratios (<0.706). Several occurrences of Proterozoic rocks are located within or next to the WCCB, and they reveal the character of the Bouguer anomaly. On the east, at Los Filtros, Proterozoic rocks crop out in a basement cored uplift interpreted to having been derived from the WCCB during the Ouachita orogeny. At Sierra La Mojina boulders of 1.1 Ga granites are found in Permian conglomerates. And at Basasiachic, xenoliths of 1.1 Ga granites are present in ash flow tuffs. Establishment of the Precambrian character of the WCCB is of importance, and these multiple occurrences are evidence. Prior studies of the Sierra Madre Occidental suggest that the region was uplifted because of a vast Cenozoic batholith presumed to lie under the SLIP (Silicic Large Igneous Province), the Upper Volcanic Series. The present study challenges that conclusion and maintains the SMO is underlain by Proterozoic silicic crust. The geology of age dated samples supports this. The WCCB is surrounded on three sides by Active Zones or Mobile Belts, which have been active extensional and translational zones periodically over a long period of time. On the east are the Paleozoic Pedrogosa Basin, Mesozoic Chihuahua Trough and Cenozoic Rio Grande Rift, the first two of which also continue around the northern border

  14. Shape Preferred Orientation of Porphyroclasts in the Active Gouge Zones of the San Andreas Fault at SAFOD

    NASA Astrophysics Data System (ADS)

    Sills, D. W.; Chester, J. S.; Chester, F. M.

    2009-12-01

    Recovered core samples from the San Andreas Fault Observatory at Depth (SAFOD) offer a unique opportunity to study the products of faulting and to learn about the mechanisms of slip at 3 km depth. Active creep is occurring at two locations in the borehole that correspond to meters-thick intervals of gouge recovered by coring. Both gouge layers consist of a clay-bearing, ultrafine grain matrix containing porphyroclasts of sandstone and serpentinite; these layers correspond to the southwest creeping zone at 3194 m measured depth (MD) and the main creeping zone at 3301 m MD. We have used X-ray Computed Tomography (XCT) imaging to investigate the mesoscale structure of the core samples, specifically to characterize the shape, preferred orientation, and size distribution of the porphyroclasts. Using various image processing techniques, porphyroclast shape and size are characterized in 3D by best-fit ellipsoids. The resolution of the XCT imaging to date permits characterization of porphyroclasts with equivalent spherical diameters greater than 8 mm; current work involves higher resolution imaging of representative samples to investigate the 3D shape of porphyroclasts to the sub-millimeter size. The porphyroclast population in each gouge layer can be approximated with a scalene-oblate ellipsoid; size and aspect ratio (major to minor axis ratios) distributions also are similar throughout. Aspect ratios generally range between 1.5 and 4, with the majority occurring between 2-2.5. A strong shape preferred orientation (SPO) exists in both creeping zones, where the minor axes form a SPO normal to the plane of the San Andreas Fault, and the major axes define a lineation in the plane of the fault. The SPO in the main creeping zone is particularly well defined, and the orientation distribution, assuming the major-axis lineation is horizontal (strike-slip kinematics), indicates a slight synthetic asymmetry relative to the macroscopic orientation of the San Andreas Fault. The

  15. Land use influence on 3-D distribution of soil microbiological activity in forest-steppe zone of Central Russia

    NASA Astrophysics Data System (ADS)

    Vasenev, Viacheslav; Tembo, Alan; Sarzhanov, Dmirty; Sotnikova, Julia; Ryzhkov, Oleg; Lakeev, Pavel; Valentini, Riccardo

    2014-05-01

    Land use is the principal factor influencing soil environmental functions and quality. Quite a few studies on soil quality mainly focus on natural and agroecosystems. Much less is known about urban ecosystems, although the urbanization effect on soil quality can be considerable. Parameters of soil microbiological activity are very sensitive to land-use change. Microbial biomass carbon (Cmic), basal respiration (BR) and microbial metabolic coefficient (qCO2) are among most widely used parameters of soil microbiological activity. They are directly associated with such soil functions as fertility, microorganisms' habitat and participation in carbon cycle. So far, most of the studies focus on the effect of land-use change on the topsoil (0-10 cm) microbiological activity, averaged for different land-use types. Much less is known about changes in spatial variability and profile distribution of Cmic, BR and qCO2 in response to different land-use. Land-use influence on spatial and profile distribution of soil microbiological activity may differ between bioclimatic zones. Very fertile and rich in carbon Chernozemic soils (depth of the A horizon up to 1 m, carbon concentration up to 7-9%), dominating in forest-steppe zone are among the most sensitive to land-use change. This study aims to improve understanding of land-use influence on 3-D distribution of Cmic, BR and qCO2 in Central Chernozemic region of Russia. We observed three land-use types (fallow land, natural pasture and meadow) located in Kursk region and three contrast urban functional zone (industrial, residential and recreational) in Kursk city. Soil samples were collected by auguring in five replicas per land-use type, four layers each sampling point (0-10, 10-50, 50-100 and 100-150 cm). Cmic, BR and qCO2 as well as Corg, N and pHKCl were analyzed in all the samples. Cmic (µg C g-1 soil) was analyzed based on the substrate induced respiration (SIR) approach. qCO2 (μg CO2-C mg-1 Cmic h-1) was calculated as the

  16. The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium

    PubMed Central

    Lier, Johanna Maria; Burmühl, Stephan; Struchtrup, Andreas; Deutschmann, Kathleen; Vetter, Maik; Leu, Tristan; Reeg, Sandra; Grune, Tilman; Rüther, Ulrich

    2015-01-01

    Mutations in RPGRIP1L result in severe human diseases called ciliopathies. To unravel the molecular function of RPGRIP1L, we analyzed Rpgrip1l−/− mouse embryos, which display a ciliopathy phenotype and die, at the latest, around birth. In these embryos, cilia-mediated signaling was severely disturbed. Defects in Shh signaling suggested that the Rpgrip1l deficiency causes an impairment of protein degradation and protein processing. Indeed, we detected a cilia-dependent decreased proteasomal activity in the absence of Rpgrip1l. We found different proteasomal components localized to cilia and identified Psmd2, a component of the regulatory proteasomal 19S subunit, as an interaction partner for Rpgrip1l. Quantifications of proteasomal substrates demonstrated that Rpgrip1l regulates proteasomal activity specifically at the basal body. Our study suggests that Rpgrip1l controls ciliary signaling by regulating the activity of the ciliary proteasome via Psmd2. PMID:26150391

  17. Strontium-90 activity concentration in soil samples from the exclusion zone of the Fukushima daiichi nuclear power plant.

    PubMed

    Sahoo, Sarata Kumar; Kavasi, Norbert; Sorimachi, Atsuyuki; Arae, Hideki; Tokonami, Shinji; Mietelski, Jerzy Wojciech; Łokas, Edyta; Yoshida, Satoshi

    2016-01-01

    The radioactive fission product (90)Sr has a long biological half-life (˜18 y) in the human body. Due to its chemical similarity to calcium it accumulates in bones and irradiates the bone marrow, causing its high radio-toxicity. Assessing (90)Sr is therefore extremely important in case of a nuclear disaster. In this work 16 soil samples were collected from the exclusion zone (<30 km) of the earthquake-damaged Fukushima Daiichi nuclear power plant, to measure (90)Sr activity concentration using liquid scintillation counting. (137)Cs activity concentration was also measured with gamma-spectroscopy in order to investigate correlation with (90)Sr. The (90)Sr activity concentrations ranged from 3.0 ± 0.3 to 23.3 ± 1.5 Bq kg(-1) while the (137)Cs from 0.7 ± 0.1 to 110.8 ± 0.3 kBq kg(-1). The fact that radioactive contamination originated from the Fukushima nuclear accident was obvious due to the presence of (134)Cs. However, (90)Sr contamination was not confirmed in all samples although detectable amounts of (90)Sr can be expected in Japanese soils, as a background, stemming from global fallout due to the atmospheric nuclear weapon tests. Correlation analysis between (90)Sr and (137)Cs activity concentrations provides a potentially powerful tool to discriminate background (90)Sr level from its Fukushima contribution. PMID:27048779

  18. Strontium-90 activity concentration in soil samples from the exclusion zone of the Fukushima daiichi nuclear power plant

    NASA Astrophysics Data System (ADS)

    Sahoo, Sarata Kumar; Kavasi, Norbert; Sorimachi, Atsuyuki; Arae, Hideki; Tokonami, Shinji; Mietelski, Jerzy Wojciech; Łokas, Edyta; Yoshida, Satoshi

    2016-04-01

    The radioactive fission product 90Sr has a long biological half-life (˜18 y) in the human body. Due to its chemical similarity to calcium it accumulates in bones and irradiates the bone marrow, causing its high radio-toxicity. Assessing 90Sr is therefore extremely important in case of a nuclear disaster. In this work 16 soil samples were collected from the exclusion zone (<30 km) of the earthquake-damaged Fukushima Daiichi nuclear power plant, to measure 90Sr activity concentration using liquid scintillation counting. 137Cs activity concentration was also measured with gamma-spectroscopy in order to investigate correlation with 90Sr. The 90Sr activity concentrations ranged from 3.0 ± 0.3 to 23.3 ± 1.5 Bq kg‑1 while the 137Cs from 0.7 ± 0.1 to 110.8 ± 0.3 kBq kg‑1. The fact that radioactive contamination originated from the Fukushima nuclear accident was obvious due to the presence of 134Cs. However, 90Sr contamination was not confirmed in all samples although detectable amounts of 90Sr can be expected in Japanese soils, as a background, stemming from global fallout due to the atmospheric nuclear weapon tests. Correlation analysis between 90Sr and 137Cs activity concentrations provides a potentially powerful tool to discriminate background 90Sr level from its Fukushima contribution.

  19. Strontium-90 activity concentration in soil samples from the exclusion zone of the Fukushima daiichi nuclear power plant

    PubMed Central

    Sahoo, Sarata Kumar; Kavasi, Norbert; Sorimachi, Atsuyuki; Arae, Hideki; Tokonami, Shinji; Mietelski, Jerzy Wojciech; Łokas, Edyta; Yoshida, Satoshi

    2016-01-01

    The radioactive fission product 90Sr has a long biological half-life (˜18 y) in the human body. Due to its chemical similarity to calcium it accumulates in bones and irradiates the bone marrow, causing its high radio-toxicity. Assessing 90Sr is therefore extremely important in case of a nuclear disaster. In this work 16 soil samples were collected from the exclusion zone (<30 km) of the earthquake-damaged Fukushima Daiichi nuclear power plant, to measure 90Sr activity concentration using liquid scintillation counting. 137Cs activity concentration was also measured with gamma-spectroscopy in order to investigate correlation with 90Sr. The 90Sr activity concentrations ranged from 3.0 ± 0.3 to 23.3 ± 1.5 Bq kg−1 while the 137Cs from 0.7 ± 0.1 to 110.8 ± 0.3 kBq kg−1. The fact that radioactive contamination originated from the Fukushima nuclear accident was obvious due to the presence of 134Cs. However, 90Sr contamination was not confirmed in all samples although detectable amounts of 90Sr can be expected in Japanese soils, as a background, stemming from global fallout due to the atmospheric nuclear weapon tests. Correlation analysis between 90Sr and 137Cs activity concentrations provides a potentially powerful tool to discriminate background 90Sr level from its Fukushima contribution. PMID:27048779

  20. Investigating Aquatic Dead Zones

    ERIC Educational Resources Information Center

    Testa, Jeremy; Gurbisz, Cassie; Murray, Laura; Gray, William; Bosch, Jennifer; Burrell, Chris; Kemp, Michael

    2010-01-01

    This article features two engaging high school activities that include current scientific information, data, and authentic case studies. The activities address the physical, biological, and chemical processes that are associated with oxygen-depleted areas, or "dead zones," in aquatic systems. Students can explore these dead zones through both…

  1. Nitrogen transformations as inferred from the activities of key enzymes in the Arabian Sea oxygen minimum zone

    NASA Astrophysics Data System (ADS)

    Shailaja, M. S.; Narvekar, P. V.; Alagarsamy, R.; Naqvi, S. W. A.

    2006-06-01

    Vertical distributions of the potential activities of some key enzymes mediating nitrification and denitrification were investigated within the oxygen (O 2) minimum zone of the Arabian Sea at a number of locations between latitudes 17°N and 21°N and longitudes 63°E and 68°E so as to get an insight into the predominant biochemical mode(s) of production and consumption of nitrous oxide (N 2O). Results revealed that the dissimilatory nitrate (NO -3) reduction activity was generally very low or absent within the σ θ range 26.6-26.8, which corresponds to the Persian Gulf Watermass (PGW). Depth profiles of nitrate reductase (NaR), nitrite reductase (NiR) and ammonia monooxygenase (AMO) activities were compared with those of O 2, NO -3, nitrite (NO -2) and N 2O, and it is concluded that nitrifier denitrification rather than heterotrophic denitrification is active within the core of PGW. The presence of multiple peaks of AMO activity coinciding with distinct maxima in the O 2 profile and with a trend opposite to that of NaR activity indicates that the two processes, viz., classical and nitrifier denitrification, occur in discrete layers, probably determined by the variations in the ambient O 2 concentrations at various depths surrounding the PGW core. Further, it appears that at the depths where nitrifier denitrification is active in the absence of heterotrophic denitrification, N 2O builds up as its consumption may be inhibited by O 2. Possible reasons for the occurrence of appreciable nitrate deficit within the core of PGW, where dissimilatory NO -3 reduction is lacking, are discussed.

  2. Arsenic Removal from Drinking Water by Adsorptive Media - U.S. EPA Demonstration Project at Covered Wells in Tohono O’odham Nation, AZ - Final Performance Evaluation Report

    EPA Science Inventory

    This report documents the activities performed and the results obtained from the arsenic removal treatment technology demonstration project at Covered Wells in Tohono O’odham Nation, AZ. The main objective of the project was to evaluate the effectiveness of AdEdge Technologies’ ...

  3. 78 FR 22843 - Foreign-Trade Zone 33-Pittsburgh, Pennsylvania, Authorization of Export Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-17

    ... 400), including notice in the Federal Register inviting public comment (77 FR 77016, 12-31-2012). The... Production Activity, Tsudis Chocolate Company (Chocolate Confectionery Bars), Pittsburgh, Pennsylvania On December 4, 2012, Tsudis Chocolate Company, submitted a notification of proposed export production...

  4. 78 FR 48647 - Foreign-Trade Zone 225-Springfield, Missouri; Authorization of Production Activity; General...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-09

    ...), including notice in the Federal Register inviting public comment (78 FR 22512, 4-16-2013). The FTZ Board has... Activity; General Dynamics Ordnance and Tactical Systems Munitions Services (Demilitarization of Munitions... Dynamics Ordnance and Tactical Systems Munitions Services, within Site 3 of FTZ 225, in Carthage,...

  5. 78 FR 73824 - Foreign-Trade Zone (FTZ) 20-Suffolk, Virginia; Notification of Proposed Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-09

    ... Production Activity; Canon Virginia, Inc. (Toner Cartridges, Toner Bottles and Cartridge Parts); Newport News... cartridges and toner bottles, within Subzone 20D. The current request would add foreign-status components to... that apply to toner cartridges, bottles and cartridge parts (duty free) for the foreign-status...

  6. 77 FR 63320 - Agency Information Collection Activities: Petroleum Refineries in Foreign Trade Sub-zones

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-16

    ... review and approval in accordance with the Paperwork Reduction Act: Petroleum Refineries in Foreign Trade... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Petroleum Refineries... collection was previously published in the Federal Register (77 FR 47429) on August 8, 2012, allowing for...

  7. 78 FR 7394 - Foreign-Trade Zone 121-Albany, NY; Authorization of Production Activity; Albany Molecular...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-01

    ... notice in the Federal Register inviting public comment (77 FR 63290, 10/16/2012). The FTZ Board has... Molecular Research, Inc.; Subzone 121A (Pharmaceutical Chemicals Production); Rensselaer, NY On September 26, 2012, Albany Molecular Research, Inc., submitted a notification of proposed production activity to...

  8. CONTROL OF BIOLOGICALLY ACTIVE DEGRADATION ZONES BY VERTICAL HETEROGENEITY: APPLICATIONS IN FRACTURED MEDIA

    EPA Science Inventory

    The U.S. Department of Energy (DOE) is faced with cleaning up wastes from reactor and weapons production activities during the latter half of this century. Many DOE sites have contaminants that are difficult to access due to depth and complex geology and are challenging to degrad...

  9. The twilight zone: ambient light levels trigger activity in primitive ants

    PubMed Central

    Narendra, Ajay; Reid, Samuel F.; Hemmi, Jan M.

    2010-01-01

    Many animals become active during twilight, a narrow time window where the properties of the visual environment are dramatically different from both day and night. Despite the fact that many animals including mammals, reptiles, birds and insects become active in this specific temporal niche, we do not know what cues trigger this activity. To identify the onset of specific temporal niches, animals could anticipate the timing of regular events or directly measure environmental variables. We show that the Australian bull ant, Myrmecia pyriformis, starts foraging only during evening twilight throughout the year. The onset occurs neither at a specific temperature nor at a specific time relative to sunset, but at a specific ambient light intensity. Foraging onset occurs later when light intensities at sunset are brighter than normal or earlier when light intensities at sunset are darker than normal. By modifying ambient light intensity experimentally, we provide clear evidence that ants indeed measure light levels and do not rely on an internal rhythm to begin foraging. We suggest that the reason for restricting the foraging onset to twilight and measuring light intensity to trigger activity is to optimize the trade-off between predation risk and ease of navigation. PMID:20129978

  10. 78 FR 13857 - Foreign-Trade Zone 84-Houston, TX; Notification of Proposed Production Activity; Toshiba...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ...; Toshiba International Corporation (Hybrid Electric Vehicle Motors and Generators Production) The Port of... generators for hybrid electric vehicles (HEV). Pursuant to 15 CFR 400.14(b), FTZ activity would be limited to... customs entry procedures that apply to electric motors and generators (duty rates range from free to...

  11. Oxygen activity dependence of the chromium (IV) population in chromium-doped forsterite crystals grown by the floating zone technique

    NASA Astrophysics Data System (ADS)

    Mass, J. L.; Burlitch, J. M.; Markgraf, S. A.; Higuchi, M.; Dieckmann, R.; Barber, D. B.; Pollock, C. R.

    1996-08-01

    The lasing of Cr-doped forsterite, Cr:Mg 2SiO 4, at 1.2 μm has been attributed to the presence of Cr 4+. The goal of this work was to determine the {Cr4+}/{Cr3+} ratios of crystals grown at different oxygen partial pressures and to compare them with a model based on point defect thermodynamics. Cr:forsterite crystals were grown by the floating zone technique with gas atmospheres of 100% Ar, 99% Ar + 1% O 2, 80% Ar + 20% O 2, 33% Ar + 67% O 2, and 100% O 2 at 1 atm total pressure. The {Cr4+}/{Cr3+} ratios, as well as the distribution of the Cr 3+ cations on the M1 and M2 sites, were determined by electron paramagnetic resonance techniques. The fraction of Cr present as Cr 4+ ions as a function of the oxygen activity of the growth environment was well described by the model.

  12. Earthquake swarm activity highlights crustal faulting associated with the Waimangu-Rotomahana-Mt Tarawera geothermal field, Taupo Volcanic Zone

    NASA Astrophysics Data System (ADS)

    Bannister, Stephen; Sherburn, Steven; Bourguignon, Sandra

    2016-03-01

    The Waimangu-Rotomahana-Mt.Tarawera geothermal field (WRTGF) in the Taupo Volcanic Zone, New Zealand, experiences frequent but sporadic earthquake swarms with durations of less than 1 day. Here we examine detailed locations of the seismic activity using precise double-difference relative location techniques. We utilize a combination of cross-correlation-derived arrival times and catalogue-based arrival times from 582 earthquakes recorded in the area between 2004 and 2015 for the relocation analysis. The new earthquake locations highlight a ~ 6 km long NE-SW lineation, which we infer to represent a sub-surface fault that extends along the northern side of Waimangu geothermal system and the north-western end of Lake Rotomahana. We suggest that this structural feature acts as a permeable pathway for aqueous fluid and CO2 release up to the surface geothermal field and Lake Rotomahana, from a deeper magmatic source.

  13. Deep Seismic Researches Of Seismic-Active Zones With Use Of High-Power Vibrators - Technique, Outcomes, Outlooks

    NASA Astrophysics Data System (ADS)

    Soloviev, V.; Seleznev, V.; Emanov, A.; Sal`Nikov, A.; Kashun, V.; Glinsky, B.; Kovalevsky, V.; Zhemchugova, I.; Danilov, I.; Liseikin, A.

    2004-12-01

    There are presented the materials of deep vibroseism researches, carried out in seismic active regions of Siberia with use of stationary (100-tos power) and moveable vibration sources (40-60tons power) and mobile digital recording equipment. There are given some examples of unique, have no world analogues, correlograms from high-power vibrators on distances to 400km and more. Using new vibroseismic technology of deep seismic researches, there were got detail deep sections of the Earth's crust and upper mantle, including time-sections of CDP-DSS up to depth of 80km. Materials of vibroseismic investigations on 2500km of seismic profiles in hard-to-reach regions of the Altay-Sayan region, the Baikal rift zone and Okhotsko-Chukotski regions are evidence of high cost efficiency, ecological safety, possibility to be realized in hard-to-reach region and finally of availability of deep seismic investigations with use of high-power vibration sources.

  14. Protein mutated in paroxysmal dyskinesia interacts with the active zone protein RIM and suppresses synaptic vesicle exocytosis

    PubMed Central

    Shen, Yiguo; Ge, Woo-Ping; Li, Yulong; Hirano, Arisa; Lee, Hsien-Yang; Rohlmann, Astrid; Missler, Markus; Tsien, Richard W.; Jan, Lily Yeh; Fu, Ying-Hui; Ptáček, Louis J.

    2015-01-01

    Paroxysmal nonkinesigenic dyskinesia (PNKD) is an autosomal dominant episodic movement disorder precipitated by coffee, alcohol, and stress. We previously identified the causative gene but the function of the encoded protein remains unknown. We also generated a PNKD mouse model that revealed dysregulated dopamine signaling in vivo. Here, we show that PNKD interacts with synaptic active zone proteins Rab3-interacting molecule (RIM)1 and RIM2, localizes to synapses, and modulates neurotransmitter release. Overexpressed PNKD protein suppresses release, and mutant PNKD protein is less effective than wild-type at inhibiting exocytosis. In PNKD KO mice, RIM1/2 protein levels are reduced and synaptic strength is impaired. Thus, PNKD is a novel synaptic protein with a regulatory role in neurotransmitter release. PMID:25730884

  15. Characterization Activities to Determine the Extent of DNAPL in the Vadose Zone at the A-014 Outfall of A/M Area

    SciTech Connect

    Jackson, D.G.

    2000-09-05

    The purpose of this investigation was to perform characterization activities necessary to confirm the presence and extent of DNAPL in the shallow vadose zone near the headwaters of the A-014 Outfall. Following the characterization, additional soil vapor extraction wells and vadose monitoring probes were installed to promote and monitor remediation activities in regions of identified DNAPL.

  16. Real time electromagnetic monitoring system used for short-term earthquakes forecast related to the seismic-active Vrancea zone

    NASA Astrophysics Data System (ADS)

    Stanica, Dumitru; Armand Stanica, Dragos

    2016-04-01

    The existence of the pre-seismic electromagnetic signals related to the earthquakes is still under scientific debate and requires new reliable information about their possible inter-relationship. In this paper, to obtain new insights into the seismic active Vrancea zone (Romania), a 3-D magnetotelluric imaging has been used to strengthen the connection between the geodynamic model and a possible generation mechanism of the intermediate depth earthquakes. Consequently, it is considered that before an earthquake initiation, due to the torsion effect, a high stress reached inside the seismogenic volume that may generates dehydration and rupture processes of the rocks, associated with the fluid migration through the lithospheric faults system, what leads to the resistivity changes. These changes have been investigated by using ULF electromagnetic data recorded in real time at the Geodynamic Observatory Provita de Sus (GOPS), placed on the Carpathian Electrical Conductivity Anomaly (CECA) at about 100km far from the seismic active Vrancea zone. The daily mean distribution of the normalized function Bzn(f) = Bz(f)/Bperp(f) (where: Bz is vertical component of the geomagnetic field; Bperp is geomagnetic component perpendicular to strike; f is frequency in Hz) and its standard deviation are performed by using a FFT band-pass filter analysis in the ULF range 0.001Hz to 0.0083Hz, for which a 2-D geoelectrical structure under GOPS has been identified. To provide reliable information in anticipating the likelihood occurrence of an earthquake of Mw higher than 4, a statistical analysis based on standardized random variable equation has been used to identify the anomalous intervals on the new time series (Bzn*) carried out in a span of three years (2013-2015). The final conclusion is that the Bzn* shows a significant anomalous effect some days (weeks) before an impending earthquake and it should be used for short-term earthquakes forecast.

  17. Analysis of radar images of the active volcanic zone at Krafla, Iceland: The effects of look azimuth biasing

    NASA Technical Reports Server (NTRS)

    Garvin, J. B.; Williams, R. S., Jr.

    1989-01-01

    The geomorphic expression of Mid-Ocean-Ridge (MOR) volcanism in a subaerial setting occurs uniquely on Earth in Iceland, and the most recent MOR eruptive activity has been concentrated in the Northeastern Volcanic Zone in an area known as Krafla. Within the Krafla region are many of the key morphologic elements of MOR-related basaltic volcanism, as well as volcanic explosion craters, subglacial lava shields, tectonic fissure swarms known as gjar, and basaltic-andesite flows with well developed ogives (pressure-ridges). The objective was to quantify the degree to which the basic volcanic and structural features can be mapped from directional SAR imagery as a function of the look azimuth. To accomplish this, the current expression of volcanic and tectonic constructs was independently mapped within the Krafla region on the E, W, and N-looking SAR images, as well as from SPOT Panchromatic imagery acquired in 1987. The initial observations of the E, W, and N images indicates that fresh a'a lava surfaces are extremely radar bright (rough at 3 cm to meter scales) independent of look direction; this suggests that these flows do not have strong flow direction related structures at meter and cm scales, which is consistent with typical Icelandic a'a lava surfaces in general. The basic impression from a preliminary analysis of the effects of look azimuth biasing on interpretation of the geology of an active MOR volcanic zone is that up to 30 percent of the diagnostic features can be missed at any given look direction, but that having two orthogonal look direction images is probably sufficient to prevent gross misinterpretation.

  18. Late Quaternary Activity and Seismogenic Potential of the Gonave microplate: South Coast Fault Zone of Southern Jamaica

    NASA Astrophysics Data System (ADS)

    Benford, B.; Mann, P.; Prentice, C.; King, W.; Wiggins-Grandison, M.; Demets, C.; Tikoff, B.

    2008-12-01

    The South Coast fault zone (SCFZ) strikes east-west and forms a scarp as high as 600 m along the southern coast of Jamaica. It has been postulated that this fault acts as a left-lateral, strike-slip 'bypass' fault that truncates the large, right-stepping restraining bend formed between the Plantain Garden fault zone of southeastern Jamaica and the Duanvale-Walton fault zone of northwestern Jamaica. GPS measurements near the SCFZ show anomalously rotated vectors consistent with active left-lateral shear. Anomalous topography along the trace of the SCFZ includes two, doubly plunging anticlines: Kemp's Hill (119 m), an isolated high in the otherwise flat Vere Plain, and Round Hill (333 m), a larger high directly adjacent to the coast. Field work identified the most active trace of the SCFZ in a notch along the north flank of Round Hill; this trace can be extrapolated to the west along the coast and east that locally defines a low scarp in alluvium. Channel profiles constructed for six rivers and streams crossing the projected trace of the SCFZ show convex-upward morphologies, consistent with dominance of tectonic uplift over river downcutting. To better define the subsurface location of the SCFZ beneath the Vere Plain, a gravity survey network consisting of 327 stations and covering an areas of 500 km2 was performed using a Lacoste and Romberg G-meter. Differential GPS allowed centimeter-level elevation control for each station. Gravity corrections (elevation, latitude, instrument drift, and earth tides) were made using QC Tool software, and topographic and terrane corrections were made using both local topographic measurements and high-resolution SRTM data. An ~20 mgal negative gravity anomaly on the otherwise flat gravity field of the Vere Plain corresponds with the projected trace of the SCFZ across the Vere Plain and the locations of one river offset. We interpret that the SCFZ has down-to-the-south throw, which has led to thickening of Quaternary sediments south

  19. Study and comparison of the maximum stress directions and main fault orientations in some active zones in Iran

    NASA Astrophysics Data System (ADS)

    Forouhid, Khatereh; Faraji, Atefeh; Ghorashi, Manouchehr

    2010-05-01

    Study and comparison of the maximum stress directions and main fault orientations in some active zones in Iran Khatereh Forouhid, Manouchehr Ghorashi, Atefeh Faraji Institute of Geophysics, Tehran University, Tehran, Iran kforouhid@yahoo.com Farajiatefeh@yahoo.com The Iranian plateau is the widest active zone in Alpine-Himalayan collision system that is located between two stable platforms, the Arabia in southwest and Eurasia in northeast. The convergence of these two platforms towards each other is the main reason for seismicity and different styles of deformation observed in Iran. In this study, the Iranian plateau is divided into 7 regions based on their seismotectonic characteristics. These regions are; Zagros, Makran, East Iran, Alborz, Kopeh Dagh, Central Iran and Azarbayejan (northwest of Iran). In each region, focal mechanism solutions of early and modern instrumental earthquakes (the only source of information suitable to use for stress distribution study in Iran) with magnitudes more than 5.0 and their relations to active faults are considered. By studying each maximum stress direction based on a group of earthquake focal mechanisms and considering main fault orientations, each region is studied individually. According to these data, some of these regions are divided into smaller parts. These sub-divided parts have some characters that make them different from their neighbors in the same region. In this regard, Zagros is studied in detail based on seismotectonic characteristics and divided into three parts, with N-S maximum stress direction (compressional) in one part and two different kind of NE-SW direction in two other. We use this information to investigate the style and distribution of active faulting in the Zagros and the relationships of this activity with shortening of the Arabia-Eurasia collision. It is worth to mention that as the fault slip will almost occur in the direction of maximum resolved shear stress on the fault plane, probably strain

  20. Estimation of ground water residence times in the Critical zone: insight from U activity ratios

    NASA Astrophysics Data System (ADS)

    Chabaux, Francois; Ackerer, Julien; Lucas, Yann; viville, Daniel

    2016-04-01

    The use of radioactive disequilibria as tracers and chronometers of weathering processes and related mass transfers has been recognized since the 60'. The development, over the last two decades, of analytical methods for measuring very precisely U-series nuclides (especially, 234U, 230Th and 226Ra) in environmental samples has opened up new scientific applications in Earth Surface Sciences. Here, we propose to present the potential of U activity ratios in surface waters as chronometer of water transfers at a watershed scale. This will be illustrated from studies performed at different scales, with the analysis of U activity ratios in surface waters from small watersheds (Strengbach and Ringelbach watersheds in the Vosges Mountain, France) but also from watersheds of much more regional extension (e.g., the Upper Rhine basin or the Ganges basin). These various studies show that variations of U activity ratios in surface waters are mainly associated with 234U-238U fractionations occurring during the water transfer within the bedrock, which intensity depends on two main parameters: the petro-physical characteristics of the aquifer, principally the geometry of water-rock interfaces and the duration of the water-rock interactions. This readily explains why different U activity ratios (UAR) can be observed in the different aquifers of a continental hydrosystem and hence why UAR can be used to trace the source of river waters. For a hydrological system developed on a substratum marked by fairly homogeneous petro-physical characteristics, the main parameter controlling the UAR in waters draining such a system would be the duration of the water-rock interactions. Variations of UAR in stream or spring waters of such a system can therefore be modeled using simple reactive transport model, which allows the estimation of both the dissolution rate of the bedrock and the residence time of the waters within the aquifer.

  1. Evidence for a subventricular zone neural stem cell phagocytic activity stimulated by the vitamin K-dependent factor protein S.

    PubMed

    Ginisty, Aurélie; Gély-Pernot, Aurore; Abaamrane, Loubna; Morel, Franck; Arnault, Patricia; Coronas, Valérie; Benzakour, Omar

    2015-02-01

    Neural stem cells, whose major reservoir in the adult mammalian brain is the subventricular zone (SVZ), ensure neuropoiesis, a process during which many generated cells die. Removal of dead cells and debris by phagocytes is necessary for tissue homeostasis. Using confocal and electron microscopy, we demonstrate that cultured SVZ cells phagocytose both 1 and 2 µm latex beads and apoptotic cell-derived fragments. We determine by flow cytometry that phagocytic cells represent more than 10% of SVZ cultured cells. Phenotyping of SVZ cells using nestin, GFAP, Sox2, or LeX/SSEA and quantification of aldehyde dehydrogenase (ALDH) activity, reveals that cells with neural stem-cell features phagocytose and represent more than 30% of SVZ phagocytic cells. In vivo, nestin-, Sox2-, and ALDH-expressing neural stem-like cells engulfed latex beads or apoptotic cell-derived fragments that were injected into mice lateral brain ventricles. We show also that SVZ cell phagocytic activity is an active process, which depends both on cytoskeleton dynamic and on recognition of phosphatidylserine eat-me signal, and is stimulated by the vitamin K-dependent factor protein S (ProS). ProS neutralizing antibodies inhibit SVZ cell phagocytic activity and exposure of SVZ cells to apoptotic cell-derived fragments induces a transient Mer tyrosine kinase receptor (MerTK) phosphorylation. Conversely, MerTK blocking antibodies impair both basal and ProS-stimulated SVZ cell phagocytic activity. By revealing that neural stem-like cells act within the SVZ neurogenic niche as phagocytes and that the ProS/MerTK path represents an endogenous regulatory mechanism for SVZ cell phagocytic activity, the present report opens-up new perspectives for both stem cell biology and brain physiopathology. PMID:25308179

  2. A New Perspective of the Radio Bright Zone at The Galactic Center: Feedback from Nuclear Activities

    NASA Astrophysics Data System (ADS)

    Zhao, Jun-Hui; Morris, Mark R.; Goss, W. M.

    2016-02-01

    New observations of Sgr A have been carried out with the Jansky VLA in the B and C arrays using the broadband (2 GHz) continuum mode at 5.5 GHz. The field of view covers the central 13‧ (30 pc) region of the radio-bright zone at the Galactic center. Using the multi-scale and multi-frequency-synthesis (MS-MFS) algorithms in CASA, we have imaged Sgr A with a resolution of 1″, achieving an rms noise of 8 μJy beam-1, and a dynamic range of 100,000:1. Both previously known and newly identified radio features in this region are revealed, including numerous filamentary sources. The radio continuum image is compared with Chandra X-ray images, with a CN emission-line image obtained with the Submillimeter Array and with detailed Paschen-α images obtained with Hubble Space Telescope/NICMOS. We discuss several prominent features in the radio image. The “Sgr A west Wings” extend 2‧ (5 pc) from the NW and SE tips of the Sgr A west H ii region (the “Mini-spiral”) to positions located 2.9 and 2.4 arcmin to the northwest and southeast of Sgr A*, respectively. The NW wing, along with several other prominent features, including the previously identified “NW Streamers,” form an elongated radio lobe (NW lobe), oriented nearly perpendicular to the Galactic plane. This radio lobe, with a size of 6.‧3 × 3.‧2 (14.4 pc × 7.3 pc), has a known X-ray counterpart. In the outer region of the NW lobe, a row of three thermally emitting rings is observed. A field containing numerous amorphous radio blobs extends for a distance of ˜2 arcmin beyond the tip of the SE wing; these newly recognized features coincide with the SE X-ray lobe. Most of the amorphous radio blobs in the NW and SE lobes have Paschen-α counterparts. We propose that they have been produced by shock interaction of ambient gas concentrations with a collimated nuclear wind or an outflow that originated from within the circumnuclear disk (CND). We also discuss the possibility that the ionized wind or

  3. Bacterial biomass and activity in the marginal ice zone of the northern Barents Sea

    NASA Astrophysics Data System (ADS)

    Tammert, Helen; Olli, Kalle; Sturluson, Maria; Hodal, Helene

    2008-10-01

    Bacteria in the Arctic Waters are well adapted to low temperatures and play a key role in the transformation of organic matter. However, the activity of planktonic bacteria at cellular level remains poorly understood. In this study, we use fluorescent markers (4',6'-diamidino-2-phenylindole (DAPI), 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), Live/Dead BacLight viability kit) to discriminate between bacterial cells with a variety of physiological activities in the 0-200 m water column and sinking particles. During two field studies (July 2003 and 2004), we covered nine stations in the northern Barents Sea. The median bacterial abundance (DAPI staining) in the upper 50 m layer was 0.9×10 6 cells ml -1 (range 0.2-3.2×10 6 cells ml -1) in 2003 and 0.5×10 6 cells ml -1 (range 0.2-1.0×10 6 cells ml -1) in 2004. Bacteria with sufficient electron transport activity to be stained with CTC were on average 10% of the total count and ca. 20% of the total cells had intact cell membranes. In the water column, proxies of substrate availability (POC, PON, chlorophyll a, primary production) and bacterial production (thymidine and leucine uptake) correlated strongly with total bacterial count, CTC-stained cells and cells with 'leaky' membrane (stained with propidium iodine), but not with the concentration of cells with intact cell membrane. Contrary to expectations, the proportion of CTC-stained bacteria was not higher in the sinking particles (captured with sediment traps) compared to the ambient water. However, out of the bacteria with intact cell membranes, a higher proportion scored as CTC positive in the aggregates compared to the ambient water. Bacterial cells with 'leaky' cell membranes formed the largest part of total cell count in all samples, and accumulated in sites with high microbial activity (sinking aggregates, chlorophyll maxima, layers of high primary and bacterial production). We hypothesize that the source of the bacterial cells with 'leaky' cell membranes

  4. Histone variant H2A.Z.2 mediates proliferation and drug sensitivity of malignant melanoma

    PubMed Central

    Vardabasso, Chiara; Gaspar-Maia, Alexandre; Hasson, Dan; Pünzeler, Sebastian; Valle-Garcia, David; Straub, Tobias; Keilhauer, Eva C.; Strub, Thomas; Dong, Joanna; Panda, Taniya; Chung, Chi-Yeh; Yao, Jonathan L.; Singh, Rajendra; Segura, Miguel F.; Fontanals-Cirera, Barbara; Verma, Amit; Mann, Matthias; Hernando, Eva; Hake, Sandra B.; Bernstein, Emily

    2015-01-01

    SUMMARY Histone variants are emerging as key regulatory molecules in cancer. Here we report a novel role for the H2A.Z isoform H2A.Z.2 as a driver of malignant melanoma. H2A.Z.2 is highly expressed in metastatic melanoma, correlates with decreased patient survival, and is required for cellular proliferation. Our integrated genomic analyses reveal that H2A.Z.2 controls the transcriptional output of E2F target genes in melanoma cells. These genes are highly expressed and display a distinct signature of H2A.Z occupancy. We identify BRD2 as an H2A.Z interacting protein, whose levels are also elevated in melanoma. We further demonstrate that H2A.Z.2 regulated genes are bound by BRD2 and E2F1 in a H2A.Z.2-dependent manner. Importantly, H2A.Z.2 deficiency sensitizes melanoma cells to chemotherapy and targeted therapies. Collectively, our findings implicate H2A.Z.2 as a mediator of cell proliferation and drug sensitivity in malignant melanoma, holding translational potential for novel therapeutic strategies. PMID:26051178

  5. Superplastic Properties of AZ31 and AZ31-1.0Y-1.3Sr Alloy Produced by Twin-Roll Casting and Sequential Hot Rolling

    NASA Astrophysics Data System (ADS)

    Ning, Huiyan; Yu, Yandong; Lin, Kai; Wen, Lihua; Liu, Chunxiang

    2016-02-01

    Superplastic mechanical properties of the AZ31 and AZ31-1.0Y-1.3Sr magnesium alloy sheets produced by twin-roll casting and sequential hot rolling (TRC) were investigated. The AZ31-1.0Y-1.3Sr alloy sheets with the thickness of 1 mm were prepared by twin-roll casting process, which exhibited finer equiaxed grain structure. Uniaxial tensile testing and gas blow forming on AZ31 and AZ31-1.0Y-1.3Sr magnesium alloy sheets were carried out. Results show that the superplastic mechanical properties of AZ31-1.0Y-1.3Sr alloys are better than those of AZ31 alloys at 400 °C and the strain rate of 7 × 10-4/s. The addition of Y and Sr elements is helpful to improve the formability of AZ31 alloy. Grain boundary sliding plays a dominant role in superplastic forming.

  6. Heavy metal concentrations and enzymatic activities in the functional zone sediments of Haizhou Bay, Lianyungang, Jiangsu, China.

    PubMed

    Li, Yu; Liu, Fu-cheng

    2015-11-01

    Surface sediments were collected at 31 sites covering five functional zones of Haizhou Bay, Lianyungang, Jiangsu, China. Heavy metal concentrations and enzymatic activity of phosphatase and urease were determined on a dry-weight basis of sediments. Metal concentrations in sediments were comparable to the Chinese National Standard of Marine Sediment Quality and were as follows: Cu, 8.60-55.8 mg kg(-1); Zn, 107-384 mg kg(-1); Pb, 33.6-200 mg kg(-1); Cd, 0.24-2.57 mg kg(-1); Cr, 30.3-92.1 mg kg(-1); As, 12.9-110 mg kg(-1); Ni, 15.8-49.6 mg kg(-1); Mn, 379-1272 mg kg(-1); and Fe, 13,790-38,240 mg kg(-1). A geoaccumulation index (I geo) was calculated to help researchers understand the status of pollutants in the sediments. I geo showed that Cd and As contamination existed in the study area. The mobility of the metals and the relationship between heavy metal concentrations of chemical fractions and enzymatic activities were also investigated. Results showed that Cd and Mn had higher mobility than other metals, and enzymatic activities may play an important role in controlling the bioavailability and transformation trend of heavy metals from one fraction to another in sediments. PMID:26431704

  7. Autoreactive marginal zone B cells are spontaneously activated but lymph node B cells require T cell help

    PubMed Central

    Mandik-Nayak, Laura; Racz, Jennifer; Sleckman, Barry P.; Allen, Paul M.

    2006-01-01

    In K/BxN mice, arthritis is induced by autoantibodies against glucose-6-phosphate-isomerase (GPI). To investigate B cell tolerance to GPI in nonautoimmune mice, we increased the GPI-reactive B cell frequency using a low affinity anti-GPI H chain transgene. Surprisingly, anti-GPI B cells were not tolerant to this ubiquitously expressed and circulating autoantigen. Instead, they were found in two functionally distinct compartments: an activated population in the splenic marginal zone (MZ) and an antigenically ignorant one in the recirculating follicular/lymph node (LN) pool. This difference in activation was due to increased autoantigen availability in the MZ. Importantly, the LN anti-GPI B cells remained functionally competent and could be induced to secrete autoantibodies in response to cognate T cell help in vitro and in vivo. Therefore, our study of low affinity autoreactive B cells reveals two distinct but potentially concurrent mechanisms for their activation, of which one is T cell dependent and the other is T cell independent. PMID:16880262

  8. Preliminary Assessment of the Nuclide Migration from the Activation Zone Around the Proposed Spallation Neutron Source Facility

    SciTech Connect

    Dole, L.R.

    1998-09-01

    The purpose of this study is to investigate the potential impacts of migrating radionuclides from the activation zone around the proposed Spallation Neutron Source (SNS). Using conservatively high estimates of the potential inventory of radioactive activation products that could form in the proposed compacted-soil shield berm around an SNS facility on the Oak Ridge Reservation (ORR), a conservative, simplified transport model was used to estimate the potential worst-case concentrations of the 12 long-lived isotopes in the groundwater under a site with the hydrologic characteristics of the ORR. Of the 12, only 3 isotopes showed any potential to exceed the U.S. Nuclear Regulatory Commission (NRC) 10 Code of Federal Regulations (CFR) Part 20 Drinking Water Limits (DWLs). These isotopes were 14C, 22Na, and 54Mn. The latter two activation products have very short half-lives of 2.6 years and 0.854 year, respectively. Therefore, these will decay before reaching an off-site receptor, and they cannot pose off-site hazards. However, for this extremely conservative model, which overestimates the mobility of the contaminant, 14C, which has a 5,730-year half-life, was shown to represent a potential concern in the context of this study's conservative assumptions. This study examines alternative modifications to the SNS shield berm and makes recommendations.

  9. Multilayer stress from gravity and its tectonic implications in urban active fault zone: A case study in Shenzhen, South China

    NASA Astrophysics Data System (ADS)

    Xu, Chuang; Wang, Hai-hong; Luo, Zhi-cai; Ning, Jin-sheng; Liu, Hua-liang

    2015-03-01

    It is significant to identify urban active faults for human life and social sustainable development. The ordinary methods to detect active faults, such as geological survey, artificial seismic exploration, and electromagnetic exploration, are not convenient to be carried out in urban area with dense buildings. It is also difficult to supply information about vertical extension of the deeper faults by these methods. Gravity, reflecting the mass distribution of the Earth's interior, provides an alternative way to detect faults, which is more efficient and convenient for urban active fault detection than the aforementioned techniques. Based on the multi-scale decomposition of gravity anomalies, a novel method to invert multilayer horizontal tectonic stresses is proposed. The inverted multilayer stress fields are further used to infer the distribution and stability of the main faults. In order to validate our method, the multilayer stress fields in the Shenzhen fault zone are calculated as a case study. The calculated stress fields show that their distribution is controlled significantly by the strike of the main faults and can be used to derive depths of the faults. The main faults in Shenzhen may range from 4 km to 20 km in the depth. Each layer of the crust is nearly equipressure since the horizontal tectonic stress has small amplitude. It indicates that the main faults in Shenzhen are relatively stable and have no serious impact on planning and construction of the city.

  10. Seismically Articulating Kilauea Volcano's Active Conduits, Rift Zones, and Faults through HVO's Second Fifty Years

    NASA Astrophysics Data System (ADS)

    Okubo, P.; Nakata, J.; Klein, F.; Koyanagi, R.; Thelen, W.

    2011-12-01

    While seismic monitoring of active Hawaiian volcanoes began 100 years ago, the build-up of the U. S. Geological Survey's (USGS) Hawaiian Volcano Observatory (HVO) seismographic network to its current configuration began in 1955, when Jerry Eaton established remote stations that telemetered data via landline to recorders at HVO. With network expansion through the 1960's, earthquake location and cataloging capabilities have evolved to afford a computer processed seismic catalog now spanning fifty years. Location accuracy and catalog completeness to smaller magnitudes have increased. Research and insights developed using HVO's seismic record have exploited the ability to seismically monitor volcanic activity at depth, to identify active regions within the volcanoes on the basis of computed hypocentral locations, to infer regions of magma storage by recognizing different families of volcanic earthquakes, and to forecast volcanic activity in both short and longer term from seismicity patterns. HVO's seismicity catalog was central to calculations of probabilistic seismic hazards. The ability to develop and implement additional analytical and interpretive capabilities has kept pace with improvements in both field and laboratory hardware and software. While the basic capabilities continue as part of HVO's core monitoring, additional interpretive capabilities now include adding details of volcanic and earthquake source regions, and viewing seismic data in juxtaposition with other observatory data streams. As HVO looks to its next century of volcano studies, research and development continue to shape the future. Broadband seismic recording at HVO has enabled extensive study by Chouet, Dawson, and co-workers of the relationship of very-long-period seismic sources beneath Kilauea's summit caldera to magma supply and transport. Recent upgrades have improved the ability to use these data in seismic cataloging and research. Data processing upgrades have bolstered the ability to

  11. Temporal dynamics of active Archaea in oxygen-depleted zones of two deep lakes.

    PubMed

    Hugoni, Mylène; Domaizon, Isabelle; Taib, Najwa; Biderre-Petit, Corinne; Agogué, Hélène; Galand, Pierre E; Debroas, Didier; Mary, Isabelle

    2015-04-01

    Deep lakes are of specific interest in the study of archaeal assemblages as chemical stratification in the water column allows niche differentiation and distinct community structure. Active archaeal community and potential nitrifiers were investigated monthly over 1 year by pyrosequencing 16S rRNA transcripts and genes, and by quantification of archaeal amoA genes in two deep lakes. Our results showed that the active archaeal community patterns of spatial and temporal distribution were different between these lakes. The meromictic lake characterized by a stable redox gradient but variability in nutrient concentrations exhibited large temporal rearrangements of the dominant euryarchaeal phylotypes, suggesting a variety of ecological niches and dynamic archaeal communities in the hypolimnion of this lake. Conversely, Thaumarchaeota Marine Group I (MGI) largely dominated in the second lake where deeper water layers exhibited only short periods of complete anoxia and constant low ammonia concentrations. Investigations conducted on archaeal amoA transcripts abundance suggested that not all lacustrine Thaumarchaeota conduct the process of nitrification. A high number of 16S rRNA transcripts associated to crenarchaeal group C3 or the Miscellaneous Euryarchaeotic Group indicates the potential for these uncharacterized groups to contribute to nutrient cycling in lakes. PMID:25472601

  12. Shh and ZRS enhancer colocalisation is specific to the zone of polarising activity.

    PubMed

    Williamson, Iain; Lettice, Laura A; Hill, Robert E; Bickmore, Wendy A

    2016-08-15

    Limb-specific Shh expression is regulated by the (∼1 Mb distant) ZRS enhancer. In the mouse, limb bud-restricted spatiotemporal Shh expression occurs from ∼E10 to E11.5 at the distal posterior margin and is essential for correct autopod formation. Here, we have analysed the higher-order chromatin conformation of Shh in expressing and non-expressing tissues, both by fluorescence in situ hybridisation (FISH) and by chromosome conformation capture (5C). Conventional and super-resolution light microscopy identified significantly elevated frequencies of Shh/ZRS colocalisation only in the Shh-expressing regions of the limb bud, in a conformation consistent with enhancer-promoter loop formation. However, in all tissues and at all developmental stages analysed, Shh-ZRS spatial distances were still consistently shorter than those to a neural enhancer located between Shh and ZRS in the genome. 5C identified a topologically associating domain (TAD) over the Shh/ZRS genomic region and enriched interactions between Shh and ZRS throughout E11.5 embryos. Shh/ZRS colocalisation, therefore, correlates with the spatiotemporal domain of limb bud-specific Shh expression, but close Shh and ZRS proximity in the nucleus occurs regardless of whether the gene or enhancer is active. We suggest that this constrained chromatin configuration optimises the opportunity for the active enhancer to locate and instigate the expression of Shh. PMID:27402708

  13. Identification of active root zone by data assimilation techniques: monitoring and modelling of irrigation experiments

    NASA Astrophysics Data System (ADS)

    Busato, Laura; Vanella, Daniela; Boaga, Jacopo; Manoli, Gabriele; Marani, Marco; Putti, Mario; Consoli, Simona; Binley, Andrew M.; Cassiani, Giorgio

    2015-04-01

    The identification of active root distribution and the quantification of relevant water fluxes (root water uptake-RWU) are key elements in understanding the exchanges of mass and energy in soil-plant-atmosphere systems. In this contribution we present the assimilation of 3D time-lapse Electrical Resistivity Tomography (ERT) data, acquired around an orange tree during irrigation experiments, in a soil-plant model that accounts for soil moisture dynamics and root water uptake (RWU), whole plant transpiration, and leaf-level photosynthesis. The model is based on a numerical solution to the 3D Richards equation modified to account for a 3D RWU, trunk xylem, and stomatal conductances. The data assimilation procedure, assisted also by independent information concerning the soil properties, aims specifically at identifying the distribution and strength of active roots modelled as sinks in the unsaturated flow model. In addition the flow model is enhanced by a forward electrical current model in order to predict the electrical response measured by ERT in dependence of the soil water content distribution. Strengths and weaknesses of the proposed approach are discussed.

  14. Shh and ZRS enhancer colocalisation is specific to the zone of polarising activity

    PubMed Central

    Williamson, Iain; Lettice, Laura A.; Hill, Robert E.

    2016-01-01

    Limb-specific Shh expression is regulated by the (∼1 Mb distant) ZRS enhancer. In the mouse, limb bud-restricted spatiotemporal Shh expression occurs from ∼E10 to E11.5 at the distal posterior margin and is essential for correct autopod formation. Here, we have analysed the higher-order chromatin conformation of Shh in expressing and non-expressing tissues, both by fluorescence in situ hybridisation (FISH) and by chromosome conformation capture (5C). Conventional and super-resolution light microscopy identified significantly elevated frequencies of Shh/ZRS colocalisation only in the Shh-expressing regions of the limb bud, in a conformation consistent with enhancer-promoter loop formation. However, in all tissues and at all developmental stages analysed, Shh-ZRS spatial distances were still consistently shorter than those to a neural enhancer located between Shh and ZRS in the genome. 5C identified a topologically associating domain (TAD) over the Shh/ZRS genomic region and enriched interactions between Shh and ZRS throughout E11.5 embryos. Shh/ZRS colocalisation, therefore, correlates with the spatiotemporal domain of limb bud-specific Shh expression, but close Shh and ZRS proximity in the nucleus occurs regardless of whether the gene or enhancer is active. We suggest that this constrained chromatin configuration optimises the opportunity for the active enhancer to locate and instigate the expression of Shh. PMID:27402708

  15. Active flexural-slip faulting: A study from the Pamir-Tian Shan convergent zone, NW China

    NASA Astrophysics Data System (ADS)

    Li, Tao; Chen, Jie; Thompson, Jessica A.; Burbank, Douglas W.; Yang, Xiaodong

    2015-06-01

    The flexural-slip fault (FSF), a type of secondary fault generated by bed-parallel slip, occurs commonly and plays an important role in accommodating fold growth. Although the kinematics and mechanics of FSFs are well studied, relatively few field observations or geometric models explore its geomorphic expression. In the Pamir-Tian Shan convergent zone, NW China, suites of well-preserved FSF scarps displace fluvial terraces in the Mingyaole and Wulagen folds. Integrating interpretations of Google Earth images, detailed geologic and geomorphic mapping, and differential GPS measurements of terrace surfaces, we summarize geomorphic features that typify these faults and create kinematic models of active flexural-slip faulting. Our study indicates the following: (i) FSF scarps commonly occur near synclinal hinges, irrespective of whether (a) the dip direction of beds on either side of the hinge is unidirectional or in opposite directions, (b) the hinge is migrating or fixed, or (c) the hinge shape is narrow and angular or wide and curved. (ii) Active FSFs are likely to produce higher scarps on steeper beds, whereas lower or no topographic scarps typify gentler beds. (iii) Tilt angles of the terrace surface displaced above FSFs progressively decrease farther away from the hinge, with abrupt changes in slope coinciding with FSF scarps; the changes in tilt angle and scarp height have a predictable geometric relationship. (iv) Active FSFs can accommodate a significant fraction of total slip and play a significant role in folding deformation. (v) Active FSFs may be used to assess seismic hazards associated with active folds and associated blind thrusts.

  16. A cross-shear deformation for optimizing the strength and ductility of AZ31 magnesium alloys

    PubMed Central

    Hamad, Kotiba; Ko, Young Gun

    2016-01-01

    Magnesium alloys have recently attracted great interest due their lightweight and high specific strength. However, because of their hexagonal close-packed structure, they have few active slip systems, resulting in poor ductility and high mechanical anisotropy at room temperature. In the present work, we used a cross-shear deformation imposed by a differential speed rolling (DSR) technique to improve the room temperature strength and ductility of AZ31 magnesium alloy sheets. To introduce the cross-shear deformation, the sheets were rotated 180° around their longitudinal axis between the adjacent passes of DSR. The sheets of the AZ31 alloy subjected to the cross-shear deformation showed a uniform fine microstructure (1.2 ± 0.1 μm) with weak basal textures. The fabricated sheets showed a simultaneous high ultimate tensile strength and elongation-to-failure, i.e., ~333 MPa and ~21%, respectively. These were explained based on the structural features evolved due to the cross-shear deformation by DSR. The high strength was attributed to the uniform fine microstructure, whereas the high ductility was explained based on the basal texture weakening. PMID:27406685

  17. A cross-shear deformation for optimizing the strength and ductility of AZ31 magnesium alloys.

    PubMed

    Hamad, Kotiba; Ko, Young Gun

    2016-01-01

    Magnesium alloys have recently attracted great interest due their lightweight and high specific strength. However, because of their hexagonal close-packed structure, they have few active slip systems, resulting in poor ductility and high mechanical anisotropy at room temperature. In the present work, we used a cross-shear deformation imposed by a differential speed rolling (DSR) technique to improve the room temperature strength and ductility of AZ31 magnesium alloy sheets. To introduce the cross-shear deformation, the sheets were rotated 180° around their longitudinal axis between the adjacent passes of DSR. The sheets of the AZ31 alloy subjected to the cross-shear deformation showed a uniform fine microstructure (1.2 ± 0.1 μm) with weak basal textures. The fabricated sheets showed a simultaneous high ultimate tensile strength and elongation-to-failure, i.e., ~333 MPa and ~21%, respectively. These were explained based on the structural features evolved due to the cross-shear deformation by DSR. The high strength was attributed to the uniform fine microstructure, whereas the high ductility was explained based on the basal texture weakening. PMID:27406685

  18. A cross-shear deformation for optimizing the strength and ductility of AZ31 magnesium alloys

    NASA Astrophysics Data System (ADS)

    Hamad, Kotiba; Ko, Young Gun

    2016-07-01

    Magnesium alloys have recently attracted great interest due their lightweight and high specific strength. However, because of their hexagonal close-packed structure, they have few active slip systems, resulting in poor ductility and high mechanical anisotropy at room temperature. In the present work, we used a cross-shear deformation imposed by a differential speed rolling (DSR) technique to improve the room temperature strength and ductility of AZ31 magnesium alloy sheets. To introduce the cross-shear deformation, the sheets were rotated 180° around their longitudinal axis between the adjacent passes of DSR. The sheets of the AZ31 alloy subjected to the cross-shear deformation showed a uniform fine microstructure (1.2 ± 0.1 μm) with weak basal textures. The fabricated sheets showed a simultaneous high ultimate tensile strength and elongation-to-failure, i.e., ~333 MPa and ~21%, respectively. These were explained based on the structural features evolved due to the cross-shear deformation by DSR. The high strength was attributed to the uniform fine microstructure, whereas the high ductility was explained based on the basal texture weakening.

  19. Experimental investigation of anisotropy evolution of AZ31 magnesium alloy sheets under tensile loading

    SciTech Connect

    Tari, D. Ghaffari; Worswick, M. J.

    2011-05-04

    Increasing demand for lighter final products has created new opportunities for the application of new light weight materials. Due to high strength to density ratio and good magnetic resistance properties, magnesium alloys are good candidates to replace steel and aluminum for same application. However, limited numbers of active slip deformation mechanisms, result in a decreased formability at room temperature. Furthermore, wrought magnesium alloys have an initial crystallographic texture, remained from the prior rolling operations, which makes them highly anisotropic. In this paper, tensile tests are performed at room temperature and 200 deg. C at different strain rates and orientations relative to the rolling direction, including rolling, 30 deg., 45 deg., 60 deg. and transverse orientation. The strain rates adopted for these experiments varied from 0.001 to 1.0. The testing results show the effect of temperature on the strain rate sensitivity of AZ31 sheets. The extent of deformation is continuously recorded using two separate high temperature extensometers. The results of testing show an increase in the r-values with the plastic deformation. The strain rate sensitivity of AZ31 increased as the temperature was elevated. At higher strain rates the measured r-values are larger and the slope of its evolution with the plastic strain is steeper.

  20. Thermal Microstructural Stability of AZ31 Magnesium after Severe Plastic Deformation

    SciTech Connect

    Young, John P.; Askari, Hesam A.; Hovanski, Yuri; Heiden, Michael J.; Field, David P.

    2015-03-01

    Both equal channel angular pressing and friction stir processing have the ability to refine the grain size of twin roll cast AZ31 magnesium and potentially improve its superplastic properties. This work used isochronal and isothermal heat treatments to investigate the microstructural stability of twin roll cast, equal channel angular pressed and friction stir processed AZ31 magnesium. For both heat treatment conditions, it was found that the twin roll casted and equal channel angular pressed materials were more stable than the friction stir processed material. Calculations of the grain growth kinetics showed that severe plastic deformation processing decreased the activation energy for grain boundary motion with the equal channel angular pressed material having the greatest Q value of the severely plastically deformed materials and that increasing the tool travel speed of the friction stir processed material improved microstructural stability. The Hollomon-Jaffe parameter was found to be an accurate means of identifying the annealing conditions that will result in substantial grain growth and loss of potential superplastic properties in the severely plastically deformed materials. In addition, Humphreys’s model of cellular microstructural stability accurately predicted the relative microstructural stability of the severely plastically deformed materials and with some modification, closely predicted the maximum grain size ratio achieved by the severely plastically deformed materials.

  1. The Corrosion Protection of Magnesium Alloy AZ31B

    NASA Technical Reports Server (NTRS)

    Danford, M. D.; Mendrek, M. J.; Mitchell, M. L.; Torres, P. D.

    1997-01-01

    Corrosion rates for bare and coated Magnesium alloy AZ31B have been measured. Two coatings, Dow-23(Trademark) and Tagnite(Trademark), have been tested by electrochemical methods and their effectiveness determined. Electrochemical methods employed were the scanning reference electrode technique (SRET), the polarization resistance technique (PR) and the electrochemical impedance spectroscopy technique (EIS). In addition, general corrosion and stress corrosion methods were employed to examine the effectiveness of the above coatings in 90 percent humidity. Results from these studies are presented.

  2. Effects of interplanetary magnetic field azimuth on auroral zone and polar cap magnetic activity

    NASA Technical Reports Server (NTRS)

    Burch, J. L.

    1972-01-01

    During relatively quiet times in the period 1964-1968, AE is found to be greater when the interplanetary magnetic field (b sub IMF) is directed toward the sun in Jan., Feb., and Apr., and when B sub IMF is directed away from the sun in Oct. to Dec. Using Murmansk hourly H values and the AE components, AU and AL, it is shown that this sector dependence is present only in the negative H deviations. This observation supports the idea that negative bay magnitudes are determined chiefly by particle-produced ionization, while positive bay magnitudes are rather insensitive to increases in particle precipitation. The ratio of DP2-type magnetic activity in the southern polar cap to that in the northern polar cap is found to be greater by a factor of about 1.75 for B sub IMF toward the sun.

  3. ΔNp63α represses anti-proliferative genes via H2A.Z deposition

    PubMed Central

    Gallant-Behm, Corrie L.; Ramsey, Matthew R.; Bensard, Claire L.; Nojek, Ignacio; Tran, Jack; Liu, Minghua; Ellisen, Leif W.; Espinosa, Joaquín M.

    2012-01-01

    ΔNp63α is a member of the p53 family of transcription factors that functions as an oncogene in squamous cell carcinomas (SCCs). Because ΔNp63α and p53 bind virtually identical DNA sequence motifs, it has been proposed that ΔNp63α functions as a dominant-negative inhibitor of p53 to promote proliferation and block apoptosis. However, most SCCs concurrently overexpress ΔNp63α and inactivate p53, suggesting the autonomous action of these oncogenic events. Here we report the discovery of a novel mechanism of transcriptional repression by ΔNp63α that reconciles these observations. We found that although both proteins bind the same genomic sites, they regulate largely nonoverlapping gene sets. Upon activation, p53 binds all enhancers regardless of ΔNp63α status but fails to transactivate genes repressed by ΔNp63α. We found that ΔNp63α associates with the SRCAP chromatin regulatory complex involved in H2A/H2A.Z exchange and mediates H2A.Z deposition at its target loci. Interestingly, knockdown of SRCAP subunits or H2A.Z leads to specific induction of ΔNp63α-repressed genes. We identified SAMD9L as a key anti-proliferative gene repressed by ΔNp63α and H2A.Z whose depletion suffices to reverse the arrest phenotype caused by ΔNp63α knockdown. Collectively, these results illuminate a molecular pathway contributing to the autonomous oncogenic effects of ΔNp63α. PMID:23019126

  4. Iron Phosphate Glass for Vitrifying Hanford AZ102 LAW in Joule Heated and Cold Crucible Induction Melters

    SciTech Connect

    Day, Delbert E.; Brow, R. K.; Ray, C. S.; Kim, Cheol-Woon; Reis, Signo T.; Vienna, John D.; Peeler, David K.; Johnson, Fabienne; Hansen, E. K.; Sevigny, Gary J.; Soelberg, Nicolas R.; Pegg, Ian L.; Gan, Hao

    2012-01-05

    An iron phosphate composition for vitrifying a high sulfate (~17 wt%) and high alkali (~80 wt%) low activity Hanford waste, known as AZ102 LAW, has been developed for processing in a Joule Heated Melter (JHM) or a Cold Crucible Induction Melter (CCIM). This composition produced a glass waste form, designated as MS26AZ102F-2, with a waste loading of 26 wt% of the AZ102 which corresponded to a total alkali and sulfate (SO3) content of 21 and 4.2 wt%, respectively. A slurry (7M Na) of MS26AZ102F-2 simulant was melted continuously at temperatures between 1030 and 1090°C for 10 days in a small JHM at PNNL and for 7 days in a CCIM at INL. The as-cast glasses produced in both melters and in trial laboratory experiments along with their CCC-treated counterparts met the DOE LAW requirements for the Product Consistency Test (PCT) and the Vapor Hydration Test (VHT). These glass waste forms retained up to 77 % of the SO3 (3.3 wt%), 100% of the Cesium, and 33 to 44% of the rhenium, surrogate for Tc-99, all of which either exceeded or were comparable to the retention limit for these species in borosilicate glass nuclear waste form. Analyses of commercial K-3 refractory lining and the Inconel 693 metal electrodes used in JHM indicated only minimum corrosion of these components by the iron phosphate glass. This is the first time that an iron phosphate composition (slurry feed) was melted continuously in the JHM and CCIM, thereby, demonstrating that iron phosphate glasses can be used as alternative hosts for vitrifying nuclear waste.

  5. The fluoride coated AZ31B magnesium alloy improves corrosion resistance and stimulates bone formation in rabbit model.

    PubMed

    Sun, Wei; Zhang, Guangdao; Tan, Lili; Yang, Ke; Ai, Hongjun

    2016-06-01

    This study aimed to evaluate the effect of fluorine coated Mg alloy and clarify its mechanism in bone formation. We implanted the fluorine coated AZ31B Mg alloy screw (group F) in rabbit mandibular and femur in vivo. Untreated AZ31B Mg alloy screw (group A) and titanium screw (group T) were used as control. Then, scanning electron microscopy, the spectral energy distribution analysis, hard and decalcified bone tissues staining were performed. Immunohistochemistry was employed to examine the protein expressions of bone morphogenetic protein 2 (BMP-2) and collagen type I in the vicinity of the implant. Compared with the group A, the degradation of the alloy was reduced, the rates of Mg corrosion and Mg ion release were slowed down, and the depositions of calcium and phosphate increased in the group F in the early stage of implantation. Histological results showed that fluorine coated Mg alloy had well osteogenic activity and biocompatibility. Moreover, fluoride coating obviously up-regulated the expressions of collagen type I and BMP-2. This study confirmed that the fluorine coating might improve the corrosion resistance of AZ31B Mg alloy and promote bone formation by up-regulated the expressions of collagen type I and BMP-2. PMID:27040245

  6. Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2

    SciTech Connect

    Nishibuchi, Ikuno; Suzuki, Hidekazu; Kinomura, Aiko; Sun, Jiying; Liu, Ning-Ang; Horikoshi, Yasunori; Shima, Hiroki; Kusakabe, Masayuki; Harata, Masahiko; Fukagawa, Tatsuo; Ikura, Tsuyoshi; Ishida, Takafumi; Nagata, Yasushi; Tashiro, Satoshi

    2014-07-15

    Purpose: The reorganization of damaged chromatin plays an important role in the regulation of the DNA damage response. A recent study revealed the presence of 2 vertebrate H2A.Z isoforms, H2A.Z-1 and H2A.Z-2. However, the roles of the vertebrate H2A.Z isoforms are still unclear. Thus, in this study we examined the roles of the vertebrate H2A.Z isoforms in chromatin reorganization after the induction of DNA double-strand breaks (DSBs). Methods and Materials: To examine the dynamics of H2A.Z isoforms at damaged sites, we constructed GM0637 cells stably expressing each of the green fluorescent protein (GFP)-labeled H2A.Z isoforms, and performed fluorescence recovery after photobleaching (FRAP) analysis and inverted FRAP analysis in combination with microirradiation. Immunofluorescence staining using an anti-RAD51 antibody was performed to study the kinetics of RAD51 foci formation after 2-Gy irradiation of wild-type (WT), H2A.Z-1- and H2A.Z-2-deficient DT40 cells. Colony-forming assays were also performed to compare the survival rates of WT, H2A.Z-1-, and H2A.Z-2-deficient DT40 cells with control, and H2A.Z-1- and H2A.Z-2-depleted U2OS cells after irradiation. Results: FRAP analysis revealed that H2A.Z-2 was incorporated into damaged chromatin just after the induction of DSBs, whereas H2A.Z-1 remained essentially unchanged. Inverted FRAP analysis showed that H2A.Z-2 was released from damaged chromatin. These findings indicated that H2A.Z-2 was exchanged at DSB sites immediately after the induction of DSBs. RAD51 focus formation after ionizing irradiation was disturbed in H2A.Z-2-deficient DT40 cells but not in H2A.Z-1-deficient cells. The survival rate of H2A.Z-2-deficient cells after irradiation was lower than those of WT and H2A.Z-1- DT40 cells. Similar to DT40 cells, H2A.Z-2-depleted U2OS cells were also radiation-sensitive compared to control and H2A.Z-1-depleted cells. Conclusions: We found that vertebrate H2A.Z-2 is involved in the regulation of the DNA

  7. NMDA receptors activated by subventricular zone astrocytic glutamate are critical for neuroblast survival prior to entering a synaptic network

    PubMed Central

    Platel, Jean-Claude; Dave, Kathleen A.; Gordon, Valerie; Lacar, Benjamin; Rubio, Maria E.; Bordey, Angélique

    2010-01-01

    SUMMARY Even before integrating into existing circuitry, adult-born neurons express receptors for neurotransmitters, but the intercellular mechanisms and their impact on neurogenesis remain largely unexplored. Here, we show that neuroblasts born in the postnatal subventricular zone (SVZ) acquire NMDA receptors (NMDARs) during their migration to the olfactory bulb. Along their route, neuroblasts are ensheathed by astrocyte-like cells expressing vesicular glutamate release machinery. Increasing calcium in these specialized astrocytes induced NMDAR-activity in neuroblasts and blocking astrocytic vesicular release eliminated spontaneous NMDAR-activity. Single-cell knockout of NMDARs using neonatal electroporation resulted in neuroblast apoptosis at the time of NMDAR acquisition. This cumulated in a 40% loss of neuroblasts along their migratory route demonstrating that NMDAR acquisition is critical for neuroblast survival, prior to entering a synaptic network. In addition, our findings suggest an unexpected mechanism where SVZ astrocytes use glutamate signaling through NMDARs to control the number of adult-born neurons reaching their final destination. PMID:20346761

  8. An automated continuous system for seismo-geochemical research in an active fault zone in SW Taiwan

    NASA Astrophysics Data System (ADS)

    Yang, T. F.; Hilton, D. R.; Fu, C. C.; Lai, C. W.; Liu, T. K.; Walia, V.; Lai, T. H.

    2014-12-01

    Previous studies have revealed that gas compositions of fluid samples collected from southwestern Taiwan where many hot springs and mud volcanoes are distributed along tectonic sutures show significant variation prior to and after some disaster seismic events [1]. Such variations, including radon activity, CH4/CO2, CO2/3He and 3He/4He ratios of gas compositions, are considered to be precursors of earthquakes in this area. To validate the relationship between fluid compositions and local earthquakes, a continuous monitoring station has been established at Yun-Shuei, which is an artesian well located at an active fault zone in SW Taiwan. It is equipped with a radon detector and a quadrupole mass spectrometer (QMS) for in-situ measurement of the dissolved gas composition. Data is telemetered to Taipei so we are able to monitor variations of gas composition in real time. Furthermore, we also installed a syringe pump apparatus for the retrieval and temporal analysis of helium (SPARTAH) at this station [2]. From the SPARTAH samples, we can obtain detailed time series records of He and anion concentration of the water samples at this station. After continuous measurement for a few months, this automated system has been demonstrated to be feasible for long-term continuous seismo-geochemical research in this area. [1] Yang et al. (2006) PAGEOPH, 163(4), 693-709. [2] Barry et al. (2009) G3, 10(5), DOI: 10.1029/2009GC002422.

  9. Vadose zone microbial community structure and activity in metal/radionuclide contaminated sediments. Final technical report

    SciTech Connect

    Balkwill, David L.

    2002-08-17

    This final technical report describes the research carried out during the final two months of the no-cost extension ending 11/14/01. The primary goals of the project were (1) to determine the potential for transformation of Cr(VI) (oxidized, mobile) to Cr(III) (reduced, immobile) under unsaturated conditions as a function of different levels and combinations of (a) chromium, (b) nitrate (co-disposed with Cr), and (c) molasses (inexpensive bioremediation substrate), and (2) to determine population structure and activity in experimental treatments by characterization of the microbial community by signature biomarker analysis and by RT-PCR and terminal restriction fragment length polymorphism (T-RFLP) and 16S ribosomal RNA genes. It was determined early in the one-year no-cost extension period that the T-RFLP approach was problematic in regard to providing information on the identities of microorganisms in the samples examined. As a result, it could not provide the detailed information on microbial community structure that was needed to assess the effects of treatments with chromium, nitrate, and/or molasses. Therefore, we decided to obtain the desired information by amplifying (using TR-PCR, with the same primers used for T-RFLP) and cloning 16S rRNA gene sequences from the same RNA extracts that were used for T-RFLP analysis. We also decided to use a restriction enzyme digest procedure (fingerprinting procedure) to place the clones into types. The primary focus of the research carried out during this report period was twofold: (a) to complete the sequencing of the clones, and (b) to analyze the clone sequences phylogenetically in order to determine the relatedness of the bacteria detected in the samples to each other and to previously described genera and species.

  10. Physical modeling of the formation and evolution of seismically active fault zones

    USGS Publications Warehouse

    Ponomarev, A.V.; Zavyalov, A.D.; Smirnov, V.B.; Lockner, D.A.

    1997-01-01

    Acoustic emission (AE) in rocks is studied as a model of natural seismicity. A special technique for rock loading has been used to help study the processes that control the development of AE during brittle deformation. This technique allows us to extend to hours fault growth which would normally occur very rapidly. In this way, the period of most intense interaction of acoustic events can be studied in detail. Characteristics of the acoustic regime (AR) include the Gutenberg-Richter b-value, spatial distribution of hypocenters with characteristic fractal (correlation) dimension d, Hurst exponent H, and crack concentration parameter Pc. The fractal structure of AR changes with the onset of the drop in differential stress during sample deformation. The change results from the active interaction of microcracks. This transition of the spatial distribution of AE hypocenters is accompanied by a corresponding change in the temporal correlation of events and in the distribution of event amplitudes as signified by a decrease of b-value. The characteristic structure that develops in the low-energy background AE is similar to the sequence of the strongest microfracture events. When the AR fractal structure develops, the variations of d and b are synchronous and d = 3b. This relation which occurs once the fractal structure is formed only holds for average values of d and b. Time variations of d and b are anticorrelated. The degree of temporal correlation of AR has time variations that are similar to d and b variations. The observed variations in laboratory AE experiments are compared with natural seismicity parameters. The close correspondence between laboratory-scale observations and naturally occurring seismicity suggests a possible new approach for understanding the evolution of complex seismicity patterns in nature. ?? 1997 Elsevier Science B.V. All rights reserved.

  11. Trends in intrusive and eruptive activity during Kilauea's long-lived east rift zone eruption

    NASA Astrophysics Data System (ADS)

    Orr, T. R.; Patrick, M. R.; Heliker, C.

    2011-12-01

    Kilauea Volcano's Pu`u `O`o eruption, continuing for nearly three decades, offers a unique opportunity to study trends in eruptive behavior. One such trend, that of uprift intrusion ± eruption, accompanied by crater floor collapse and eruptive hiatus, has been repeated several times at Pu`u `O`o. This includes the February 7, 1993, intrusion; the January 29, 1997, intrusion and eruption; the September 12, 1999, intrusion; and the June 17, 2007, intrusion and eruption. Activity resumed within Pu`u `O`o following each of these events, and crater refilling culminated eventually in the outbreak of lava from new vents on the flank of the Pu`u `O`o cone. The pattern was repeated again in 2011, when a brief fissure eruption uprift from Pu`u `O`o started on March 5. The Pu`u `O`o crater floor dropped about 115 m in response to the eruption, which ceased on March 9. After a short hiatus, lava reappeared in Pu`u `O`o on March 26, and the crater began to fill slowly thereafter by overflow from a central lava lake. Starting in late June 2011, however, the crater floor began to uplift in a wholesale fashion, suggesting an increase in the pressure beneath the Pu`u `O`o edifice. By late July, the lava within the crater had reached its highest level since early 2004, and lava had begun to overflow from the southwestern side of the crater. On August 3, the west side of the Pu`u `O`o cone was abruptly thrust upward as a sill was injected beneath that portion of the cone. Within minutes, lava began to erupt from a crack on the west flank of Pu`u `O`o, completing the pattern of intrusion, crater collapse, refilling, and breakout. During a long-lived eruption, maintaining a detailed observational and geophysical record is essential for recognizing patterns that may emerge. Recognizing such a pattern allowed Hawaiian Volcano Observatory scientists to prepare a response well in advance of the August 3, 2011 event, and provides guidance for responding to future eruption crises at Pu`u `O

  12. An integrated approach to the seismic activity and structure of the central Lesser Antilles subduction megathrust seismogenic zone

    NASA Astrophysics Data System (ADS)

    Hirn, Alfred; Laigle, Mireille; Charvis, Philippe; Flueh, Ernst; Gallart, Josep; Kissling, Edi; Lebrun, Jean-Frederic; Nicolich, Rinaldo; Sachpazi, Maria

    2010-05-01

    In order to increase the understanding of plate boundaries that show currently low seismic activity, as was the Sumatra-Andaman subduction before the major earthquake in 2004, a cluster of surveys and cruises has been carried out in 2007 and coordinated under the European Union THALES WAS RIGHT project on the Lesser Antilles subduction zone of the Carribean-America plate boundary. A segment of the corresponding transform boundary just tragically ruptured in the 2010 January 12, Haïti earthquake. This cluster is composed by the German cruise TRAIL with the vessel F/S M. A. MERIAN, the French cruise SISMANTILLES II with the IFREMER vessel N/O ATALANTE), and French cruise OBSANTILLES with the IRD vessel N/O ANTEA. During these cruises and surveys, 80 OBS, Ocean Bottom Seismometers, 64 of which with 3-components seismometers and hydrophones, and 20 OBH with hydrophones have been brought together from several pools (Geoazur Nice, INSU/IPGP Paris, IfM-GEOMAR Kiel, AWI Bremerhaven), with up to 30 land stations (CSIC Barcelona, IPG Paris, INSU-RLBM and -Lithoscope, ETH Zurich). The deployment of all these instruments has been supported principally in addition by ANR Catastrophes Telluriques et Tsunamis (SUBSISMANTI), by the EU SALVADOR Programme of IFM-GEOMAR, as well as by the EU project THALES WAS RIGHT. The main goal of this large seismic investigation effort is the understanding of the behaviour of the seismogenic zone and location of potential source regions of mega-thrust earthquakes. Specific goals are the mapping of the subduction interplate in the range where it may be seismogenic along the Lesser Antilles Arc from Antigua to Martinique Islands, as a contribution to identification and localisation in advance of main rupture zones of possible future major earthquakes, and to the search for transient signals of the activity. The forearc region, commonly considered as a proxy to the seismogenic portion of the subduction mega-thrust fault plane, and which is here the

  13. Pollution from organic contaminants in Greek marine areas, receiving anthropogenic pressures from intense activities in the coastal zone

    NASA Astrophysics Data System (ADS)

    Hatzianestis, Ioannis

    2014-05-01

    Polycyclic aromatic hydrocarbons (PAHs) are widespread pollutants in marine sediments, receiving the pressures from various anthropogenic activities in the coastal zone. Due to their mutagenic and carcinogenic behaviour, PAHs are classified as priority contaminants to be monitored in environmental quality control schemes. The purpose of this study was to determine the levels of PAHs in coastal areas of Greece directly influenced from the operation of major industrial units in the coastal zone, investigate their sources and evaluate their potential toxicity by comparison against effect - based sediment quality guidelines. Thirty two surface sediment samples were collected from three areas of the Hellenic coastline: a) Antikyra bay in Korinthiakos gulf, influenced from the operation of an alumina and aluminium production plant b) Larymna bay in Noth Evoikos gulf, influenced from the operation of a nickel production plant and c) Aliveri bay in South Evoikos Gulf, influenced from a cement production plant. In all the areas studied, aquaculture and fishing activities have been also developed in the coastal zone. PAH concentrations were determined by GC-MS, after soxhlet extraction and fractionation by silica column chromatography. PAH sources and origin were investigated by applying several isomeric ratio diagnostic criteria. The mean quotient Effect- Range Median (m-ERM) was used to evaluate the potential of adverse effects posed to benthic organisms. Three m-ERM-q values were used to differentiate the probability of observing toxicity and classify sites into four categories: sediments with m-ERM<0.1 have the lowest probability (9%) of being toxic, those with m-ERM from 0.11 to 0.5 have a 21% probability of toxicity, those with m-ERM from 0.51 to 1.5 a 49% probability of toxicity, while sediments with m-ERM >1.5 have the highest probability (76%) of toxicity. Extremely high PAH concentrations more than 100,000 ng/g were found in the close vicinity of the alumina

  14. Ventilation rates and activity levels of juvenile jumbo squid under metabolic suppression in the oxygen minimum zone.

    PubMed

    Trübenbach, Katja; Pegado, Maria R; Seibel, Brad A; Rosa, Rui

    2013-02-01

    The Humboldt (jumbo) squid, Dosidicus gigas, is a part-time resident of the permanent oxygen minimum zone (OMZ) in the Eastern Tropical Pacific and, thereby, it encounters oxygen levels below its critical oxygen partial pressure. To better understand the ventilatory mechanisms that accompany the process of metabolic suppression in these top oceanic predators, we exposed juvenile D. gigas to the oxygen levels found in the OMZ (1% O(2), 1 kPa, 10 °C) and measured metabolic rate, activity cycling patterns, swimming mode, escape jet (burst) frequency, mantle contraction frequency and strength, stroke volume and oxygen extraction efficiency. In normoxia, metabolic rate varied between 14 and 29 μmol O(2) g(-1) wet mass h(-1), depending on the level of activity. The mantle contraction frequency and strength were linearly correlated and increased significantly with activity level. Additionally, an increase in stroke volume and ventilatory volume per minute was observed, followed by a mantle hyperinflation process during high activity periods. Squid metabolic rate dropped more than 75% during exposure to hypoxia. Maximum metabolic rate was not achieved under such conditions and the metabolic scope was significantly decreased. Hypoxia changed the relationship between mantle contraction strength and frequency from linear to polynomial with increasing activity, indicating that, under hypoxic conditions, the jumbo squid primarily increases the strength of mantle contraction and does not regulate its frequency. Under hypoxia, jumbo squid also showed a larger inflation period (reduced contraction frequency) and decreased relaxed mantle diameter (shortened diffusion pathway), which optimize oxygen extraction efficiency (up to 82%/34%, without/with consideration of 60% potential skin respiration). Additionally, they breathe 'deeply', with more powerful contractions and enhanced stroke volume. This deep-breathing behavior allows them to display a stable ventilatory volume per

  15. Multiscale seismic imaging of active fault zones for hazard assessment: A case study of the Santa Monica fault zone, Los Angeles, California

    USGS Publications Warehouse

    Pratt, T.L.; Dolan, J.F.; Odum, J.K.; Stephenson, W.J.; Williams, R.A.; Templeton, M.E.

    1998-01-01

    High-resolution seismic reflection profiles at two different scales were acquired across the transpressional Santa Monica Fault of north Los Angeles as part of an integrated hazard assessment of the fault. The seismic data confirm the location of the fault and related shallow faulting seen in a trench to deeper structures known from regional studies. The trench shows a series of near-vertical strike-slip faults beneath a topographic scarp inferred to be caused by thrusting on the Santa Monica fault. Analysis of the disruption of soil horizons in the trench indicates multiple earthquakes have occurred on these strike-slip faults within the past 50 000 years, with the latest being 1000 to 3000 years ago. A 3.8-km-long, high-resolution seismic reflection profile shows reflector truncations that constrain the shallow portion of the Santa Monica Fault (upper 300 m) to dip northward between 30?? and 55??, most likely 30?? to 35??, in contrast to the 60?? to 70?? dip interpreted for the deeper portion of the fault. Prominent, nearly continuous reflectors on the profile are interpreted to be the erosional unconformity between the 1.2 Ma and older Pico Formation and the base of alluvial fan deposits. The unconformity lies at depths of 30-60 m north of the fault and 110-130 m south of the fault, with about 100 m of vertical displacement (180 m of dip-slip motion on a 30??-35?? dipping fault) across the fault since deposition of the upper Pico Formation. The continuity of the unconformity on the seismic profile constrains the fault to lie in a relatively narrow (50 m) zone, and to project to the surface beneath Ohio Avenue immediately south of the trench. A very high-resolution seismic profile adjacent to the trench images reflectors in the 15 to 60 m depth range that are arched slightly by folding just north of the fault. A disrupted zone on the profile beneath the south end of the trench is interpreted as being caused by the deeper portions of the trenched strike

  16. 241-AZ Tank Farm Construction Extent of Condition Review for Tank Integrity

    SciTech Connect

    Barnes, Travis J.; Boomer, Kayle D.; Gunter, Jason R.; Venetz, Theodore J.

    2013-07-30

    This report provides the results of an extent of condition construction history review for tanks 241-AZ-101 and 241-AZ-102. The construction history of the 241-AZ tank farm has been reviewed to identify issues similar to those experienced during tank AY-102 construction. Those issues and others impacting integrity are discussed based on information found in available construction records, using tank AY-102 as the comparison benchmark. In the 241-AZ tank farm, the second DST farm constructed, both refractory quality and tank and liner fabrication were improved.

  17. 77 FR 71167 - Foreign-Trade Zone 37-Orange County, New York, Authorization of Production Activity, Takasago...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-29

    ... notice in the Federal Register inviting public comment (77 FR 46377, 8-3-2012). The FTZ Board has... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 37--Orange County, New York, Authorization of...

  18. Multiblock grid generation with automatic zoning

    NASA Technical Reports Server (NTRS)

    Eiseman, Peter R.

    1995-01-01

    An overview will be given for multiblock grid generation with automatic zoning. We shall explore the many advantages and benefits of this exciting technology and will also see how to apply it to a number of interesting cases. The technology is available in the form of a commercial code, GridPro(registered trademark)/az3000. This code takes surface geometry definitions and patterns of points as its primary input and produces high quality grids as its output. Before we embark upon our exploration, we shall first give a brief background of the environment in which this technology fits.

  19. Archaeological studies at Drill Hole U20az Pahute Mesa, Nye county, Nevada. [Contains bibliography

    SciTech Connect

    Simmons, A.H.; Hemphill, M.L.; Henton, G.H.; Lockett, C.L.; Nials, F.L.; Pippin, L.C.; Walsh, L.

    1991-07-01

    During the summer of 1987, the Quaternary Sciences Center (formerly Social Science Center) of the Desert Research Institute (DRI), University of Nevada System, conducted data recovery investigations at five archaeological sites located near Drill Hole U20az on the Nevada Test Site in southern Nevada. These sites were among 12 recorded earlier during an archaeological survey of the drill hole conducted as part of the environmental compliance activities of the Department of Energy (DOE). The five sites discussed in this report were considered eligible for the National Register of Historic Places and were in danger of being adversely impacted by construction activities or by effects of the proposed underground nuclear test. Avoidance of these sites was not a feasible alternative; thus DRI undertook a data recovery program to mitigate expected adverse impacts. DRI's research plan included controlled surface collections and excavation of the five sites in question, and had the concurrence of the Nevada Division of Historic Preservation and Archaeology and the Advisory Council of Historic Preservation. Of the five sites investigated, the largest and most complex, 26Ny5207, consists of at least three discrete artifact concentrations. Sites 26Ny5211 and 26Ny5215, both yielded considerable assemblages. Site 26Ny5206 is very small and probably is linked to 26Ny5207. Site 26Ny5205 contained a limited artifact assemblage. All of the sites were open-air occurrences, and, with one exception contained no or limited subsurface cultural deposits. Only two radiocarbon dates were obtained, both from 26Ny5207 and both relatively recent. While the investigations reported in the volume mitigate most of the adverse impacts from DOE activities at Drill Hole U20az, significant archaeological sites may still exist in the general vicinity. Should the DOE conduct further activities in the region, additional cultural resource investigations may be required. 132 refs., 71 figs., 44 tabs.

  20. Late Cenozoic deformation of the Da'an-Dedu Fault Zone and its implications for the earthquake activities in the Songliao basin, NE China

    NASA Astrophysics Data System (ADS)

    Zhongyuan, Yu; Peizhen, Zhang; Wei, Min; Qinghai, Wei; Limei, Wang; Bin, Zhao; Shuang, Liu; Jian, Kang

    2015-08-01

    The Da'an-Dedu Fault Zone is a major tectonic feature cutting through the Songliao Basin from south to north in NE China. Five earthquakes with magnitudes over 5 that occurred during the past 30 years suggest the fault zone is a seismogenic structure with future seismic potential. The structural pattern, tectonic history, Quaternary activity and seismic potential have previously been unknown due to the Quaternary sedimentary coverage and lack of large historic earthquakes (M > 7). In this paper, we use seismic reflection profiles and drilling from petroleum explorations and shallow-depth seismic reflections to study those problems. The total length of the Da'an-Dedu Fault Zone is more than 400 km; modern seismicity delineates it into 4 segments each with a length of 90-100 km. In cross-section view, the folds and associated faults form a complex structural belt with a width of more than 10 km. Shallow-level seismic reflection across the Da'an-Dedu Fault Zone reveals that the Late Quaternary sediments were folded and faulted, indicating its present tectonic activity. The Da'an-Dedu Fault Zone and Songliao Basin have been subjected to three stages of tectonic evolution: a rifting stage characterized by normal faulting and extension (∼145-112 Ma), a prolonged stage of thermal subsidence (∼112-65 Ma), and a tectonic reversal that has been taking place since ∼65 Ma. Our shallow-level reflection profiles show that the folding and reverse faulting have influenced the Late Quaternary sediments. The seismicity and moderate earthquakes suggest that the tectonic activity persists today. The deformation rate across the Da'an-Dedu Fault Zone, however, is measured to be very slow. In conjunction with the inference that most deformation in NE China may be taken up by the Yilan-Yitong Fault Zone bounding the Songliao Basin to the east, we suggest moderate earthquake potential and thus moderate seismic hazards along the Da'an-Dedu Fault Zone. The geological structures, which

  1. Quantifying soil and critical zone variability in a forested catchment through digital soil mapping

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Quantifying catchment scale soil property variation yields insights into critical zone evolution and function. The objective of this study was to quantify and predict the spatial distribution of soil properties within a high elevation forested catchment in southern AZ, USA using a combined set of di...

  2. Coupling capillary zone electrophoresis with electron transfer dissociation and activated ion electron transfer dissociation for top-down proteomics.

    PubMed

    Zhao, Yimeng; Riley, Nicholas M; Sun, Liangliang; Hebert, Alexander S; Yan, Xiaojing; Westphall, Michael S; Rush, Matthew J P; Zhu, Guijie; Champion, Matthew M; Mba Medie, Felix; Champion, Patricia A DiGiuseppe; Coon, Joshua J; Dovichi, Norman J

    2015-01-01

    Top-down proteomics offers the potential for full protein characterization, but many challenges remain for this approach, including efficient protein separations and effective fragmentation of intact proteins. Capillary zone electrophoresis (CZE) has shown great potential for separation of intact proteins, especially for differentially modified proteoforms of the same gene product. To date, however, CZE has been used only with collision-based fragmentation methods. Here we report the first implementation of electron transfer dissociation (ETD) with online CZE separations for top-down proteomics, analyzing a mixture of four standard proteins and a complex protein mixture from the Mycobacterium marinum bacterial secretome. Using a multipurpose dissociation cell on an Orbitrap Elite system, we demonstrate that CZE is fully compatible with ETD as well as higher energy collisional dissociation (HCD), and that the two complementary fragmentation methods can be used in tandem on the electrophoretic time scale for improved protein characterization. Furthermore, we show that activated ion electron transfer dissociation (AI-ETD), a recently introduced method for enhanced ETD fragmentation, provides useful performance with CZE separations to greatly increase protein characterization. When combined with HCD, AI-ETD improved the protein sequence coverage by more than 200% for proteins from both standard and complex mixtures, highlighting the benefits electron-driven dissociation methods can add to CZE separations. PMID:25893372

  3. Effect of Y, Sr, and Nd additions on the microstructure and microfracture mechanism of squeeze-cast AZ91-X magnesium alloys

    SciTech Connect

    Lee, S.; Lee, S.H.; Kim, D.H.

    1998-04-01

    This study aims to investigate the effects of Y, Sr, and Nd additions on the microstructure and microfracture mechanism of the four squeeze-cast magnesium alloys based on the commercial AZ91 alloy. Microstructural observation, in situ fracture tests, fractographic observation were conducted on the alloys to clarify the microfracture process. Microstructural analyses indicated that grain refinement could be achieved by small additions of alloying elements, although the discontinuously precipitated Mg{sub 17}Al{sub 12} phases still existed on grain boundaries. From in situ fracture observation of an AZ91-Sr alloy, it was seen that coarse needle-shaped compound particles and Mg{sub 17}Al{sub 12} phases located on the grain boundary provided easy intergranular fracture sites under low stress intensity factor levels, resulting in the drop in toughness. On the other hand, the AZ91-Y and AZ91-Nd alloys showed improved fracture toughness, since deformation and fracture paths proceeded into grains rather than to grain boundaries, as the planar slip bands and twinnings actively developed inside the grains. These findings suggested, on the basis of the well-developed planar slip bands and twinnings, that the small addition of Y or Nd was very effective in improving fracture toughness.

  4. Crustal Structure Across the Okavango Rift Zone, Botswana: Initial Results From the PRIDE-SEISORZ Active-Source Seismic Profile

    NASA Astrophysics Data System (ADS)

    Canales, J. P.; Moffat, L.; Lizarralde, D.; Laletsang, K.; Harder, S. H.; Kaip, G.; Modisi, M.

    2015-12-01

    The PRIDE project aims to understand the processes of continental rift initiation and evolution by analyzing along-axis trends in the southern portion of the East Africa Rift System, from Botswana through Zambia and Malawi. The SEISORZ active-source seismic component of PRIDE focused on the Okavango Rift Zone (ORZ) in northwestern Botswana, with the main goal of imaging the crustal structure across the ORZ. This will allow us to estimate total crustal extension, determine the pattern and amount of thinning, assess the possible presence of melt within the rift zone, and assess the contrasts in crustal blocks across the rift, which closely follows the trend of a fold belt. In November 2014 we conducted a crustal-scale, 450-km-long seismic refraction/wide-angle reflection profile consisting of 19 sources (shots in 30-m-deep boreholes) spaced ~25 km apart from each other, and 900 receivers (IRIS/PASSCAL "Texan" dataloggers and 4.5Hz geophones) with ~500 m spacing. From NW to SE, the profile crosses several tectonic domains: the Congo craton, the Damara metamorphic belt and the Ghanzi-Chobe fold belt where the axis of the ORZ is located, and continues into the Kalahari craton. The record sections display clear crustal refraction (Pg) and wide-angle Moho reflection (PmP) phases for all 17 of the good-quality shots, and a mantle refraction arrival (Pn), with the Pg-PmP-Pn triplication appearing at 175 km offset. There are distinct changes in the traveltime and amplitude of these phases along the transect, and on either side of the axis, that seem to correlate with sharp transitions across tectonic terrains. Initial modeling suggests: (1) the presence of a sedimentary half-graben structure at the rift axis beneath the Okavango delta, bounded to the SE by the Kunyere-Thamalakane fault system; (2) faster crustal Vp in the domains to the NW of the ORZ; and (3) thicker crust (45-50 km) at both ends of the profile within the Congo and Kalahari craton domains than at the ORZ and

  5. Control of biologically active degradation zones by vertical heterogeneity: Applications in fractured media. 1998 annual progress report

    SciTech Connect

    Colwell, F.S.; Smith, R.; McKinley, J.P.; Fredrickson, J.K.; Onstott, T.C.; Reysenbach, A.L.

    1998-06-01

    'The objective of this research is to determine the relationship between biologically active contaminant degradation zones in a fractured, subsurface medium and vertical geological heterogeneities. The research is being performed on samples collected from the Test Area North (TAN) site at the Idaho National Engineering and Environmental Laboratory (INEEL) where a dissolved trichloroethylene (TCE) plume is migrating in the basalts and interbed sediments of the Eastern Snake River Plain (ESRP) aquifer. Results are leading to an enhanced understanding of the constraints placed on the activities and distribution of TCE-degrading organisms by the geochemical and hydrological environment. This understanding allows better decisions to be made regarding the use of remedial technologies such as natural attenuation and in-situ bioremediation at geologically complex waste sites. Through this research, investigations conducted by the DOE Subsurface Science Program at TAN have been extended in order to develop a mechanistic understanding of the coupled geomicrobial and hydrogeochemical processes that are necessary to predict field-scale intrinsic degradation rates of TCE. The research objective is being accomplished by characterizing paired cores and water samples from boreholes located in differing geochemical and flow environments within the plume. Analysis of these samples will allow the determination of the spatial correlation between microbial degradation and preferred flow paths for the contaminant and required electron donors and acceptors. A combination of traditional microbiological methods (e.g., enrichments) and molecular tools are being used to characterize the indigenous microbial communities. This report summarizes work conducted after 1.5 years of a three year project.'

  6. Soil Moisture Active Passive (SMAP) Mission Level 4 Surface and Root Zone Soil Moisture (L4_SM) Product Specification Document

    NASA Technical Reports Server (NTRS)

    Reichle, Rolf H.; Ardizzone, Joseph V.; Kim, Gi-Kong; Lucchesi, Robert A.; Smith, Edmond B.; Weiss, Barry H.

    2015-01-01

    This is the Product Specification Document (PSD) for Level 4 Surface and Root Zone Soil Moisture (L4_SM) data for the Science Data System (SDS) of the Soil Moisture Active Passive (SMAP) project. The L4_SM data product provides estimates of land surface conditions based on the assimilation of SMAP observations into a customized version of the NASA Goddard Earth Observing System, Version 5 (GEOS-5) land data assimilation system (LDAS). This document applies to any standard L4_SM data product generated by the SMAP Project. The Soil Moisture Active Passive (SMAP) mission will enhance the accuracy and the resolution of space-based measurements of terrestrial soil moisture and freeze-thaw state. SMAP data products will have a noteworthy impact on multiple relevant and current Earth Science endeavors. These include: Understanding of the processes that link the terrestrial water, the energy and the carbon cycles, Estimations of global water and energy fluxes over the land surfaces, Quantification of the net carbon flux in boreal landscapes Forecast skill of both weather and climate, Predictions and monitoring of natural disasters including floods, landslides and droughts, and Predictions of agricultural productivity. To provide these data, the SMAP mission will deploy a satellite observatory in a near polar, sun synchronous orbit. The observatory will house an L-band radiometer that operates at 1.40 GHz and an L-band radar that operates at 1.26 GHz. The instruments will share a rotating reflector antenna with a 6 meter aperture that scans over a 1000 km swath.

  7. Altered Active Zones, Vesicle Pools, Nerve Terminal Conductivity, and Morphology during Experimental MuSK Myasthenia Gravis

    PubMed Central

    Patel, Vishwendra; Oh, Anne; Voit, Antanina; Sultatos, Lester G.; Babu, Gopal J.; Wilson, Brenda A.; Ho, Mengfei; McArdle, Joseph J.

    2014-01-01

    Recent studies demonstrate reduced motor-nerve function during autoimmune muscle-specific tyrosine kinase (MuSK) myasthenia gravis (MG). To further understand the basis of motor-nerve dysfunction during MuSK-MG, we immunized female C57/B6 mice with purified rat MuSK ectodomain. Nerve-muscle preparations were dissected and neuromuscular junctions (NMJs) studied electrophysiologically, morphologically, and biochemically. While all mice produced antibodies to MuSK, only 40% developed respiratory muscle weakness. In vitro study of respiratory nerve-muscle preparations isolated from these affected mice revealed that 78% of NMJs produced endplate currents (EPCs) with significantly reduced quantal content, although potentiation and depression at 50 Hz remained qualitatively normal. EPC and mEPC amplitude variability indicated significantly reduced number of vesicle-release sites (active zones) and reduced probability of vesicle release. The readily releasable vesicle pool size and the frequency of large amplitude mEPCs also declined. The remaining NMJs had intermittent (4%) or complete (18%) failure of neurotransmitter release in response to 50 Hz nerve stimulation, presumably due to blocked action potential entry into the nerve terminal, which may arise from nerve terminal swelling and thinning. Since MuSK-MG-affected muscles do not express the AChR γ subunit, the observed prolongation of EPC decay time was not due to inactivity-induced expression of embryonic acetylcholine receptor, but rather to reduced catalytic activity of acetylcholinesterase. Muscle protein levels of MuSK did not change. These findings provide novel insight into the pathophysiology of autoimmune MuSK-MG. PMID:25438154

  8. Activity-dependent endogenous taurine release facilitates excitatory neurotransmission in the neocortical marginal zone of neonatal rats

    PubMed Central

    Qian, Taizhe; Chen, Rongqing; Nakamura, Masato; Furukawa, Tomonori; Kumada, Tatsuro; Akita, Tenpei; Kilb, Werner; Luhmann, Heiko J.; Nakahara, Daiichiro; Fukuda, Atsuo

    2014-01-01

    In the developing cerebral cortex, the marginal zone (MZ), consisting of early-generated neurons such as Cajal-Retzius cells, plays an important role in cell migration and lamination. There is accumulating evidence of widespread excitatory neurotransmission mediated by γ-aminobutyric acid (GABA) in the MZ. Cajal-Retzius cells express not only GABAA receptors but also α2/β subunits of glycine receptors, and exhibit glycine receptor-mediated depolarization due to high [Cl−]i. However, the physiological roles of glycine receptors and their endogenous agonists during neurotransmission in the MZ are yet to be elucidated. To address this question, we performed optical imaging from the MZ using the voltage-sensitive dye JPW1114 on tangential neocortical slices of neonatal rats. A single electrical stimulus evoked an action-potential-dependent optical signal that spread radially over the MZ. The amplitude of the signal was not affected by glutamate receptor blockers, but was suppressed by either GABAA or glycine receptor antagonists. Combined application of both antagonists nearly abolished the signal. Inhibition of Na+, K+-2Cl− cotransporter by 20 µM bumetanide reduced the signal, indicating that this transporter contributes to excitation. Analysis of the interstitial fluid obtained by microdialysis from tangential neocortical slices with high-performance liquid chromatography revealed that GABA and taurine, but not glycine or glutamate, were released in the MZ in response to the electrical stimulation. The ambient release of taurine was reduced by the addition of a voltage-sensitive Na+ channel blocker. Immunohistochemistry and immunoelectron microscopy indicated that taurine was stored both in Cajal-Retzius and non-Cajal-Retzius cells in the MZ, but was not localized in presynaptic structures. Our results suggest that activity-dependent non-synaptic release of endogenous taurine facilitates excitatory neurotransmission through activation of glycine receptors in

  9. Post-Supereruption (18-19 Ma) Magmatic Reactivation Beneath the Silver Creek Caldera, Black Mountains, AZ

    NASA Astrophysics Data System (ADS)

    Mcdowell, S.; Miller, C. F.; Ferguson, C.

    2011-12-01

    The Silver Creek caldera, southern Black Mountains, AZ, is the source of the supereruption that produced the Miocene (18.8 Ma) Peach Spring Tuff (PST), an extensive ignimbrite found throughout much of northwestern Arizona, southern Nevada, and southeastern California. The caldera's eastern margin is intruded by a slightly younger (18.5 +/- 0.5 Ma), ~30 km2 complex of epizonal, intermediate to felsic plutonic rocks. Because it is the largest known suite of intrusive rocks associated with the Peach Spring supereruption and contiguous (~19.5-17.5 Ma) volcanic activity in the Black Mountains, the Silver Creek intrusive complex provides a valuable record of processes operating in the shallow crust in the aftermath of a major eruption and during a period of intense volcanic activity. Rocks in the Silver Creek intrusive complex have historically been divided into two units, the Moss porphyry and the Times porphyry, though the complex exhibits textural and compositional complexity that belies a simple two-unit classification scheme. Field observations and geochemical analysis indicate that the northern portion of the Silver Creek suite comprises porphyries and coarse-grained rocks with ~62 to ~68 wt. % SiO2 ("Moss porphyry"). Rounded, 2-10 cm enclaves (59 wt. % SiO2) with crenulate margins are sparse overall but locally abundant in this portion of the complex. The southern part of the complex consists of leucogranitic porphyry and coarse-grained granite with >70 wt. % SiO2 ("Times porphyry"). At the east/west-trending Times/Moss contact zone along Silver Creek, the coarse-grained component of the Times contains < 0.5-2 m-diameter, fine-grained enclaves with crenulate margins and compositions similar to that of the intermediate Moss to the north. Mafic, intermediate, and felsic porphyritic dikes crosscut the entire complex. Major and trace element compositions of the Silver Creek intrusive complex define a coherent and continuous array extending from the most mafic enclaves

  10. Final Report - Montana State University - Microbial Activity and Precipitation at Solution-Solution Mixing Zones in Porous Media

    SciTech Connect

    Gerlach, Robin

    2014-10-31

    Background. The use of biological and chemical processes that degrade or immobilize contaminants in subsurface environments is a cornerstone of remediation technology. The enhancement of biological and chemical processes in situ, involves the transport, displacement, distribution and mixing of one or more reactive agents. Biological and chemical reactions all require diffusive transport of solutes to reaction sites at the molecular scale and accordingly, the success of processes at the meter-scale and larger is dictated by the success of phenomena that occur at the micron-scale. However, current understanding of scaling effects on the mixing and delivery of nutrients in biogeochemically dynamic porous media systems is limited, despite the limitations this imposes on the efficiency and effectiveness of the remediation challenges at hand. Objectives. We therefore proposed to experimentally characterize and computationally describe the growth, evolution, and distribution of microbial activity and mineral formation as well as changes in transport processes in porous media that receive two or more reactive amendments. The model system chosen for this project was based on a method for immobilizing 90Sr, which involves stimulating microbial urea hydrolysis with ensuing mineral precipitation (CaCO3), and co-precipitation of Sr. Studies at different laboratory scales were used to visualize and quantitatively describe the spatial relationships between amendment transport and consumption that stimulate the production of biomass and mineral phases that subsequently modify the permeability and heterogeneity of porous media. Biomass growth, activity, and mass deposition in mixing zones was investigated using two-dimensional micro-model flow cells as well as flow cells that could be analyzed using synchrotron-based x-ray tomography. Larger-scale flow-cell experiments were conducted where the spatial distribution of media properties, flow, segregation of biological activity and

  11. Homeobox NKX2-3 promotes marginal-zone lymphomagenesis by activating B-cell receptor signalling and shaping lymphocyte dynamics.

    PubMed

    Robles, Eloy F; Mena-Varas, Maria; Barrio, Laura; Merino-Cortes, Sara V; Balogh, Péter; Du, Ming-Qing; Akasaka, Takashi; Parker, Anton; Roa, Sergio; Panizo, Carlos; Martin-Guerrero, Idoia; Siebert, Reiner; Segura, Victor; Agirre, Xabier; Macri-Pellizeri, Laura; Aldaz, Beatriz; Vilas-Zornoza, Amaia; Zhang, Shaowei; Moody, Sarah; Calasanz, Maria Jose; Tousseyn, Thomas; Broccardo, Cyril; Brousset, Pierre; Campos-Sanchez, Elena; Cobaleda, Cesar; Sanchez-Garcia, Isidro; Fernandez-Luna, Jose Luis; Garcia-Muñoz, Ricardo; Pena, Esther; Bellosillo, Beatriz; Salar, Antonio; Baptista, Maria Joao; Hernandez-Rivas, Jesús Maria; Gonzalez, Marcos; Terol, Maria Jose; Climent, Joan; Ferrandez, Antonio; Sagaert, Xavier; Melnick, Ari M; Prosper, Felipe; Oscier, David G; Carrasco, Yolanda R; Dyer, Martin J S; Martinez-Climent, Jose A

    2016-01-01

    NKX2 homeobox family proteins have a role in cancer development. Here we show that NKX2-3 is overexpressed in tumour cells from a subset of patients with marginal-zone lymphomas, but not with other B-cell malignancies. While Nkx2-3-deficient mice exhibit the absence of marginal-zone B cells, transgenic mice with expression of NKX2-3 in B cells show marginal-zone expansion that leads to the development of tumours, faithfully recapitulating the principal clinical and biological features of human marginal-zone lymphomas. NKX2-3 induces B-cell receptor signalling by phosphorylating Lyn/Syk kinases, which in turn activate multiple integrins (LFA-1, VLA-4), adhesion molecules (ICAM-1, MadCAM-1) and the chemokine receptor CXCR4. These molecules enhance migration, polarization and homing of B cells to splenic and extranodal tissues, eventually driving malignant transformation through triggering NF-κB and PI3K-AKT pathways. This study implicates oncogenic NKX2-3 in lymphomagenesis, and provides a valid experimental mouse model for studying the biology and therapy of human marginal-zone B-cell lymphomas. PMID:27297662

  12. Homeobox NKX2-3 promotes marginal-zone lymphomagenesis by activating B-cell receptor signalling and shaping lymphocyte dynamics

    PubMed Central

    Robles, Eloy F.; Mena-Varas, Maria; Barrio, Laura; Merino-Cortes, Sara V.; Balogh, Péter; Du, Ming-Qing; Akasaka, Takashi; Parker, Anton; Roa, Sergio; Panizo, Carlos; Martin-Guerrero, Idoia; Siebert, Reiner; Segura, Victor; Agirre, Xabier; Macri-Pellizeri, Laura; Aldaz, Beatriz; Vilas-Zornoza, Amaia; Zhang, Shaowei; Moody, Sarah; Calasanz, Maria Jose; Tousseyn, Thomas; Broccardo, Cyril; Brousset, Pierre; Campos-Sanchez, Elena; Cobaleda, Cesar; Sanchez-Garcia, Isidro; Fernandez-Luna, Jose Luis; Garcia-Muñoz, Ricardo; Pena, Esther; Bellosillo, Beatriz; Salar, Antonio; Baptista, Maria Joao; Hernandez-Rivas, Jesús Maria; Gonzalez, Marcos; Terol, Maria Jose; Climent, Joan; Ferrandez, Antonio; Sagaert, Xavier; Melnick, Ari M.; Prosper, Felipe; Oscier, David G.; Carrasco, Yolanda R.; Dyer, Martin J. S.; Martinez-Climent, Jose A.

    2016-01-01

    NKX2 homeobox family proteins have a role in cancer development. Here we show that NKX2-3 is overexpressed in tumour cells from a subset of patients with marginal-zone lymphomas, but not with other B-cell malignancies. While Nkx2-3-deficient mice exhibit the absence of marginal-zone B cells, transgenic mice with expression of NKX2-3 in B cells show marginal-zone expansion that leads to the development of tumours, faithfully recapitulating the principal clinical and biological features of human marginal-zone lymphomas. NKX2-3 induces B-cell receptor signalling by phosphorylating Lyn/Syk kinases, which in turn activate multiple integrins (LFA-1, VLA-4), adhesion molecules (ICAM-1, MadCAM-1) and the chemokine receptor CXCR4. These molecules enhance migration, polarization and homing of B cells to splenic and extranodal tissues, eventually driving malignant transformation through triggering NF-κB and PI3K-AKT pathways. This study implicates oncogenic NKX2-3 in lymphomagenesis, and provides a valid experimental mouse model for studying the biology and therapy of human marginal-zone B-cell lymphomas. PMID:27297662

  13. Regulation of invertase activity in different root zones of wheat (Triticum aestivum L.) seedlings in the course of osmotic adjustment under water deficit conditions.

    PubMed

    Königshofer, Helga; Löppert, Hans-Georg

    2015-07-01

    Osmotic adjustment of roots is an essential adaptive mechanism to sustain water uptake and root growth under water deficit. In this paper, the role of invertases (β-fructofuranosidase, EC 3.2.1.26) in osmotic adjustment was investigated in the root tips (cell division and elongation zone) and the root maturation zone of wheat (Triticum aestivum L. cv. Josef) in the course of osmotic stress imposed by 20% polyethylene glycol (PEG) 6000. The two root zones investigated differed distinctly in the response of invertases to water deprivation. In the root tips, the activity of the vacuolar and cell wall-bound invertases increased markedly under water stress resulting in the accumulation of hexoses (glucose and fructose) that contributed significantly to osmotic adjustment. A transient rise in hydrogen peroxide (H2O2) preceded the enhancement of invertases upon exposure to osmotic stress. Treatment with the NADPH oxidase inhibitor diphenylene iodonium (DPI) abolished the stress induced H2O2 production and suppressed the stimulation of the vacuolar invertase activity, whereas the activity of the cell wall-bound invertase was not influenced by DPI. As a consequence of the inhibitory effect of DPI on the vacuolar invertase, hexose levels and osmotic adjustment were also markedly decreased in the root tips under water deficit in the presence of DPI. These data suggest that H2O2 probably generated by a NADPH oxidase is required as a signalling molecule for the up-regulation of the vacuolar invertase activity in the root tips under osmotic stress, thereby enhancing the capacity for osmotic adjustment. In the root maturation zone, an early H2O2 signal could not be detected in response to PEG application. Only an increase in the glucose level that was not paralleled by fructose and a slight stimulation of the activity of the vacuolar invertase occurred in the maturation zone after water deprivation. The stress induced accumulation of glucose in the maturation zone was not

  14. Effects of Microstructure and Processing Methods on Creep Behavior of AZ91 Magnesium Alloy

    NASA Astrophysics Data System (ADS)

    Shahbeigi Roodposhti, Peiman; Sarkar, Apu; Murty, Korukonda L.; Scattergood, Ronald O.

    2016-07-01

    This review sheds light on the creep properties of AZ91 magnesium alloys with a major emphasis on the influence of microstructure on the creep resistance and underlying creep deformation mechanism based on stress exponent and activation energy. Effects of processing routes such as steel mold casting, die casting, and thixoforming are considered. Roles of a wide range of additional alloying elements such as Si, Sb, Bi, Ca, Sn, REs, and combined addition of them on the microstructure modification were investigated. The reaction between these elements and the Mg or Al in the matrix develops some thermally stable intermetallic phases which improves the creep resistance at elevated temperatures, however does not influence the creep mechanism.

  15. Formation of Ha-Containing Coating on AZ31 Magnesium Alloy by Micro-Arc Oxidation

    NASA Astrophysics Data System (ADS)

    Tang, Hui; Li, Deyu; Chen, Xiuping; Wu, Chao; Wang, Fuping

    2013-08-01

    Magnesium and its alloys are potential biodegradable implant materials due to their attractive biological properties. But the use of magnesium is still hampered by its poor corrosion resistance in physiological fluids. In this study, a HA-containing coating was fabricated by micro-arc oxidation (MAO). The active plasma species of micro-discharge was studied by optical emission spectroscopy (OES). The microstructure and composition were analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The corrosion behavior and apatite-forming ability were studied by electrochemical tests and immersed samples in simulated body fluids (SBF). The results show that the microdischarge channel model is gas discharges and oxide layer discharges. The elements from the substrate and electrolyte take part in the formation of the coating. The MAO coating significantly improves the corrosion resistance of AZ31 magnesium alloy and enhances the apatite formation ability.

  16. Mixer pump test plan for double shell tank AZ-101

    SciTech Connect

    Symons, G.A.; Staehr, T.W., Westinghouse Hanford

    1996-06-01

    Westinghouse Hanford Company has undertaken the task to develop and demonstrate a method of retrieval for double-shell tank (DST)waste. Mixer pumps were chosen as the planned method of retrieval for the DSTs, based on engineering technology studies,past experience with hydraulic sluicing at the Hanford Site, and experience with mixer pumps at the Westinghouse Savannah River Site. This document outlines a test plan to demonstrate the ability of two 300 hp mixer pumps installed in the AZ-101 tank to mobilize waste.

  17. Modelling of Superplastic Forming of AZ31 Magnesium Alloy

    SciTech Connect

    Giuliano, G.

    2011-01-17

    In this study the constitutive equation of the superplastic AZ31 magnesium-based alloy is modelled by the power law relationship between the stress, the strain and the strain-rate and an accurate procedure for determining the constants of the material is presented. Moreover, the problem of optimizing the pressure-time load curve of a free forming process is investigated and resolved by means of a pressure jump forming process. The experimental tests, carried out to support the finite-element modelling, have shown good agreement between the numerical results and the experimental data.

  18. Model of Reentrant Ventricular Tachycardia based upon Infarct Border Zone Geometry Predicts Reentrant Circuit Features as Determined by Activation Mapping

    PubMed Central

    Ciaccio, Edward J; Ashikaga, Hiroshi; Kaba, Riyaz A; Cervantes, Daniel; Hopenfeld, Bruce; Wit, Andrew L; Peters, Nicholas S; McVeigh, Elliot R; Garan, Hasan; Coromilas, James

    2008-01-01

    Background Infarct border zone (IBZ) geometry likely affects inducibility and characteristics of postinfarction reentrant ventricular tachycardia, but the connection has not been established. Objective To determine characteristics of post infarction ventricular tachycardia in the IBZ. Methods A geometric model describing the relationship between IBZ geometry and wavefront propagation in reentrant circuits was developed. Based on the formulation, slow conduction and block was expected to coincide with areas where IBZ thickness (T) is minimal and the local spatial gradient in thickness (ΔT) is maximal, so that the degree of wavefront curvature ρ ∝ ΔT/T is maximal. Regions of fastest conduction velocity were predicted to coincide with areas of minimum ΔT. In seven arrhythmogenic postinfarction canine heart experiments, tachycardia was induced by programmed stimulation, and activation maps were constructed from multichannel recordings. IBZ thickness was measured in excised hearts from histologic analysis or magnetic resonance imaging. Reentrant circuit properties were predicted from IBZ geometry and compared with ventricular activation maps following tachycardia induction. Results Mean IBZ thickness was 231±140µm at the reentry isthmus and 1440±770µm in the outer pathway (p<0.001). Mean curvature ρ was 1.63±0.45mm−1 at functional block line locations, 0.71±0.18mm−1 at isthmus entrance-exit points, and 0.33±0.13mm−1 in the outer reentrant circuit pathway. The mean conduction velocity about the circuit during reentrant tachycardia was 0.32±0.04mm/ms at entrance-exit points, 0.42±0.13mm/ms for the entire outer pathway, and 0.64±0.16mm/ms at outer pathway regions with minimum ΔT. Model sensitivity and specificity to detect isthmus location was 75.0±5.7% and 97.2±0.7%. Conclusions Reentrant circuit features as determined by activation mapping can be predicted on the basis of IBZ geometrical relationships. PMID:17675078

  19. Extension in the Colorado Plateau/Basin and Range Transition Zone, Central Utah: An Active or Passive Process?

    NASA Astrophysics Data System (ADS)

    Hasterok, D.; Wannamaker, P. E.; Chapman, D. S.; Doerner, W.

    2007-12-01

    The Colorado Plateau-Great Basin transition zone (TZ) in central Utah is a presently extending lithospheric block composed of previously stable Proterozoic lithosphere. TZ extension may be driven by high topography resulting from overthickening during the Laramide Orogeny and passive plate boundary forces similar to the Great Basin. However, high TZ topography coupled with a thinned lithosphere may indicate dynamic mantle upwelling and active processes acting within the TZ. To investigate the active vs. passive rifting hypotheses we have collected 56 new broadband and 9 long-period MT stations to merge two existing MT lines for a combined length of 400~km (124 sites) covering the eastern Great Basin into the Colorado Plateau at a latitude of ~38.5°N. We have also collected over 300 new heat production and thermal conductivity measurements across the southwest to develop a thermal model of the eastern Basin and Range and Colorado Plateau. MT data show a semi-contiguous highly conductive body in the lower crust of the eastern Great Basin that rises to a shallow depth of <20~km beneath the TZ. This conductive layer appears to be connected to the surface by a series of symmetric rift related normal faults mapped at the surface. These normal faults may be acting as pathways for large-scale fluid connection between the upper and lower crust. MT inversion results suggest an electrical anisotropy factor >3 in the upper mantle with an enhanced conductivity in a N-S orientation that is in agreement with observations of fast seismic SKS split direction. We propose that anisotropy observations may be due to small degrees of interconnected partial melt aligned with a N-S geologic strike beneath the Basin and Range. A thermal model, combining measured thermophysical properties with existing heat flow data, MT measurements, and estimates of seismogenic depth is used to predict lithospheric thickness and mantle temperature variations along the MT profile. Lithospheric thicknesses

  20. Vertical distribution, transport, and exchange of carbon in the northeast Pacific Ocean: evidence for multiple zones of biological activity

    NASA Astrophysics Data System (ADS)

    Karl, David M.; Knauer, George A.

    1984-03-01

    A sediment trap experiment was conducted to investigate the production, decomposition, and transport of organic matter from 0 to 2000 m at a station 100 km northeast of Point Sur, California. Parameters measured included (1) rates of autotrophic production of carbon, (2) vertical depth distributions of total carbon, nitrogen, and living biomass, and (3) downward flux of organic carbon, nitrogen, ATP, RNA, and fecal pellets. Metabolic activity and microbial growth rates (RNA and DNA synthesis) were also estimated in situ, for both the 'suspended' (i.e., samples captured in standard water bottles) and 'sinking' (i.e., samples captured in sediment traps) particles. Daily depth-integrated rates of primary production averaged 564 mg C m -2, of which 10 to 15% was removed from the euphotic zone by sinking, assuming steady-state conditions. The profiles of suspended carbon, nitrogen, C:N ratios, and ATP conformed to previously published concentration-depth profiles from the region. The vertical flux profiles of organic matter, however, revealed two important features that were not evident in the suspended particulate matter profiles. First, there was an obvious mid-water depth increase (i.e., an increase in organic carbon and nitrogen flux with increasing depth) between 700 and 900 m, suggesting horizontal advection or in situ production. Similar flux profiles were also observed for ATP, RNA, and total fecal pellets. Second, the C:N ratios for the sediment trap materials collected at mid-ocean depths (600 to 1200 m) were low compared to values measured for 'suspended' particulate organic materials collected from comparable depths, supporting the in situ production hypothesis. An observed maximum in the rate of RNA and DNA synthesis for microorganisms associated with particles collected at 700 m confirmed that the flux anomalies were the result of in situ microbiological processes rather than horizontal advection. We hypothesize that the in situ activity measured at 700 m

  1. The Rhizosphere Zone: A Hot Spot of Microbial Activity and Methylmercury Production in Saltmarsh Sediments of San Francisco Bay, California

    NASA Astrophysics Data System (ADS)

    Windham-Myers, L.; Marvin-Dipasquale, M.; Voytek, M.; Kirshtein, J.; Krabbenhoft, D. P.; Agee, J. L.; Cox, M.; Kakouros, E.; Collins, J. N.; Yee, D.

    2008-12-01

    Tidal marshes of varying hydrology and salinity have been shown to have high rates of microbial methylmercury (MeHg) production, especially the periodically flooded, higher elevations which are densely vegetated with shallowly rooted plants. The specific influence of emergent wetland plants and their active rhizosphere (root zone) on mercury (Hg) biogeochemistry, however, is poorly understood. Seasonal and spatial patterns of Hg biogeochemistry were examined in 2005 and 2006 at three marshes along a salinity gradient of the Petaluma River, in Northern San Francisco Bay, California. In addition, to directly examine the influence of rhizosphere activity on MeHg production, a suite of devegetation experiments was conducted in 2006 within each marsh using paired vegetated and devegetated plots in two marsh subhabitats: poorly- drained interior sites and well-drained "edge" sites near slough channels. Surface sediment (0-2cm) was sampled in both April and August from these plots, as well as from 1st and 3rd order slough channels that were naturally free of vegetation. Vegetated marsh sites produced 3- to19-fold more MeHg than did slough sites, and MeHg production rates were greater in marsh interior sites compared to more oxic marsh "edge" sites. Microbial biomass (ng DNA gdrysed) was greater in vegetated marsh settings, compared to slough channels, and increased significantly between April and August at all marsh sites. Despite this seasonal increase in microbial biomass, MeHg concentrations and production rates decreased from April to August in vegetated surface sediments. Microbial indicators of methylation also decreased from April to August, including rates of microbial sulfate reduction and the abundance of iron- and sulfate- reducing bacterial DNA. Results from the devegetated plots suggest that root exudation of fermentative labile carbon to surface soils is responsible for the higher microbial biomass, and the higher relative abundance of iron- and sulfate

  2. Fracture bone healing and biodegradation of AZ31 implant in rats.

    PubMed

    Iglesias, C; Bodelón, O G; Montoya, R; Clemente, C; Garcia-Alonso, M C; Rubio, J C; Escudero, M L

    2015-04-01

    The ideal temporary implant should offer enough mechanical support to allow healing of the fracture and then biodegrade and be resorbed by metabolic mechanisms without causing any toxic effect. The aim of this research has been to simultaneously study in situ bone healing and the biodegradation of AZ31 Mg alloy as an osteosynthesis material. The in vivo study was carried out in AZ31 implants with and without Mg-fluoride coating inserted in un-fractured and fractured femurs of Wistar rats for long experimentation time, from 1 to 13 months, by means of computed tomography, histological and histomorphometric analysis. Tomography analysis showed the bone healing and biodegradation of AZ31 implants. The fracture is healed in 100% of the animals, and AZ31 maintains its mechanical integrity throughout the healing process. Biodegradation was monitored, quantifying the evolution of gas over time by 3D composition of tomography images. In all the studied groups, gas pockets disappear with time as a result of the diffusion process through soft tissues. Histomorphometric studies reveal that after 13 months the 46.32% of AZ31 alloy has been resorbed. The resorption of the coated and uncoated AZ31 implants inserted in fractured femurs after 1, 9 and 13 months does not have statistically significant differences. There is a balance between the biodegradation of AZ31 and bone healing which allows the use of AZ31 to be proposed as an osteosynthesis material. PMID:25886380

  3. 76 FR 2000 - Modification of Class E Airspace; Show Low, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-12

    ... airspace at Show Low, AZ, to accommodate aircraft using a new Area Navigation (RNAV) Global Positioning... Register a notice of proposed rulemaking to amend controlled airspace at Show Low, AZ (75 FR 65255... a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26,...

  4. CONCENTRATIONS OF PESTICIDE FROM DERMAL SURFACES: A COMPARISON OF NHEXAS & AZ BORDER SAMPLES

    EPA Science Inventory

    NHEXAS-AZ was a statewide survey designed to gather data on the distributions of exposure from various media. Results of intensive sampling were obtained from 179 homes. Border-AZ was a similar study focusing on homes within 40 km of the Arizona-Mexico Border; similar results...

  5. PI3 Kinase and FOXO1 Transcription Factor Activity Differentially Control B Cells in the Germinal Center Light and Dark Zones.

    PubMed

    Sander, Sandrine; Chu, Van Trung; Yasuda, Tomoharu; Franklin, Andrew; Graf, Robin; Calado, Dinis Pedro; Li, Shuang; Imami, Koshi; Selbach, Matthias; Di Virgilio, Michela; Bullinger, Lars; Rajewsky, Klaus

    2015-12-15

    Phosphatidylinositol 3' OH kinase (PI3K) signaling and FOXO transcription factors play opposing roles at several B cell developmental stages. We show here abundant nuclear FOXO1 expression in the proliferative compartment of the germinal center (GC), its dark zone (DZ), and PI3K activity, downregulating FOXO1, in the light zone (LZ), where cells are selected for further differentiation. In the LZ, however, FOXO1 was expressed in a fraction of cells destined for DZ reentry. Upon FOXO1 ablation or induction of PI3K activity, GCs lost their DZ, owing at least partly to downregulation of the chemokine receptor CXCR4. Although this prevented proper cyclic selection of cells in GCs, somatic hypermutation and proliferation were maintained. Class switch recombination was partly lost due to a failure of switch region targeting by activation-induced deaminase (AID). PMID:26620760

  6. 78 FR 17099 - Safety Zone; BWRC Southwest Showdown 2, Parker, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-20

    ... Drag Boat Association is sponsoring the Blue Water Resort and Casino Southwest Showdown 2, which is... Paperwork Reduction Act of 1995 (44 U.S.C. 3501-3520). 5. Federalism A rule has implications for...

  7. 78 FR 33703 - Safety Zone; Great Western Tube Float; Colorado River; Parker, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-05

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice... include all navigable waters of the Colorado River from La Paz County Park to the Blue Water Resort... width of the river from La Paz County Park to the Blue Water Resort & Casino. However, vessels...

  8. Abnormal Activation of RhoA/ROCK-I Signaling in Junctional Zone Smooth Muscle Cells of Patients With Adenomyosis.

    PubMed

    Wang, S; Duan, H; Zhang, Y; Sun, F Q

    2016-03-01

    Adenomyosis (ADS) is a common estrogen-dependent gynecological disease with unknown etiology. The RhoA/Rho-kinase (ROCK) signaling pathway is involved in various cellular functions, including migration, proliferation, and smooth muscle contraction. Here we examined the potential role of this pathway in junctional zone (JZ) contraction in women with and without ADS. We demonstrated that in the normal JZ, RhoA and ROCK-I messenger RNA (mRNA) and protein expression was significantly higher in the proliferative phase of the menstrual cycle than in the secretory phase. Expression of RhoA and ROCK-I in the JZ from women with ADS was significantly higher than in the control women and showed no significant differences across the menstrual cycle. Treatment of JZ smooth muscle cells (JZSMCs) with estrogen at 0, 1, 10, or 100 nmol/L for 24 hours resulted in increased expression of RhoA, ROCK-I, and myosin light-chain (MLC) phosphorylation (p-MLC) in a dose-dependent manner. In parallel to its effects on p-MLC, estrogen-mediated, dose-dependent contraction responses in JZSMCs. Estrogen-mediated contraction in the ADS group was significantly higher than in the controls and also showed no significant differences across the menstrual cycle. These effects were suppressed in the presence of ICI 182780 or Y27632, supporting an estrogen receptor-dependent and RhoA activation-dependent mechanism. Our results indicate that the level of RhoA and ROCK-I increases in patients with ADS and the cyclic change is lost. Estrogen may affect uterine JZ contraction of ADS by enhancing RhoA/ ROCK-I signaling. PMID:26335177

  9. A reduced crustal magnetization zone near the first observed active hydrothermal vent field on the Southwest Indian Ridge

    NASA Astrophysics Data System (ADS)

    Zhu, Jian; Lin, Jian; Chen, Yongshun J.; Tao, Chunhui; German, Christopher R.; Yoerger, Dana R.; Tivey, Maurice A.

    2010-09-01

    Inversion of near-bottom magnetic data reveals a well-defined low crustal magnetization zone (LMZ) near a local topographic high (37°47‧S, 49°39‧E) on the ultraslow-spreading Southwest Indian Ridge (SWIR). The magnetic data were collected by the autonomous underwater vehicle ABE on board R/V DaYangYiHao in February-March 2007. The first active hydrothermal vent field observed on the SWIR is located in Area A within and adjacent to the LMZ at the local topographic high, implying that this LMZ may be the result of hydrothermal alteration of magnetic minerals. The maximum reduction in crustal magnetization is 3 A/M. The spatial extent of the LMZ is estimated to be at least 6.7 × 104 m2, which is larger than that of the LMZs at the TAG vent field on the Mid-Atlantic Ridge (MAR), as well as the Relict Field, Bastille, Dante-Grotto, and New Field vent-sites on the Juan de Fuca Ridge (JdF). The calculated magnetic moment, i.e., the product of the spatial extent and amplitude of crustal magnetization reduction is at least -3 × 107 Am2 for the LMZ on the SWIR, while that for the TAG field on the MAR is -8 × 107 Am2 and that for the four individual vent fields on the JdF range from -5 × 107 to -3 × 107 Am2. Together these results indicate that crustal demagnetization is a common feature of basalt-hosted hydrothermal vent fields at mid-ocean ridges of all spreading rates. Furthermore, the crustal demagnetization of the Area A on the ultraslow-spreading SWIR is comparable in strength to that of the TAG area on the slow-spreading MAR.

  10. Neural progenitor cells isolated from the subventricular zone present hemichannel activity and form functional gap junctions with glial cells

    PubMed Central

    Talaverón, Rocío; Fernández, Paola; Escamilla, Rosalba; Pastor, Angel M.; Matarredona, Esperanza R.; Sáez, Juan C.

    2015-01-01

    The postnatal subventricular zone (SVZ) lining the walls of the lateral ventricles contains neural progenitor cells (NPCs) that generate new olfactory bulb interneurons. Communication via gap junctions between cells in the SVZ is involved in NPC proliferation and in neuroblast migration towards the olfactory bulb. SVZ NPCs can be expanded in vitro in the form of neurospheres that can be used for transplantation purposes after brain injury. We have previously reported that neurosphere-derived NPCs form heterocellular gap junctions with host glial cells when they are implanted after mechanical injury. To analyze functionality of NPC-glial cell gap junctions we performed dye coupling experiments in co-cultures of SVZ NPCs with astrocytes or microglia. Neurosphere-derived cells expressed mRNA for at least the hemichannel/gap junction channel proteins connexin 26 (Cx26), Cx43, Cx45 and pannexin 1 (Panx1). Dye coupling experiments revealed that gap junctional communication occurred among neurosphere cells (incidence of coupling: 100%). Moreover, hemichannel activity was also detected in neurosphere cells as evaluated in time-lapse measurements of ethidium bromide uptake. Heterocellular coupling between NPCs and glial cells was evidenced in co-cultures of neurospheres with astrocytes (incidence of coupling: 91.0 ± 4.7%) or with microglia (incidence of coupling: 71.9 ± 6.7%). Dye coupling in neurospheres and in co-cultures was inhibited by octanol, a gap junction blocker. Altogether, these results suggest the existence of functional hemichannels and gap junction channels in postnatal SVZ neurospheres. In addition, they demonstrate that SVZ-derived NPCs can establish functional gap junctions with astrocytes or microglia. Therefore, cell-cell communication via gap junctions and hemichannels with host glial cells might subserve a role in the functional integration of NPCs after implantation in the damaged brain. PMID:26528139

  11. Fault Population Analyses in the Eastern California Shear Zone: Insights into the Development of Young, Actively Evolving Plate Boundary Structures

    NASA Astrophysics Data System (ADS)

    Zhou, X.; Dawers, N. H.; Amer, R. M.

    2014-12-01

    Relationships between cumulative fault displacement, slip rate and length, along with fault population statistics are analyzed for faults located within the Eastern California Shear Zone (ECSZ), focusing on areas north of the Garlock fault. Here many faults are geologically young and in an early stage of evolution, while many older and larger faults are also still active. We analyze scaling relationships for both strike-slip and normal faults in order to determine whether the two fault populations share the same properties or not. Cumulative displacement, slip rate and length data are collected from published maps and literature sources. The dataset spans fault lengths from tens of meters to hundreds of kilometers. Results of fault scaling analyses indicate that displacement has a linear relationship with fault length for normal faults in this area over the entire length span, whereas strike-slip faults do not have a clear displacement-length scaling relation. For a given length, the subset of strike-slip faults typically exhibits a much larger displacement than that for the normal faults. The slip rate versus length trends are similar but are considerably more scattered. In addition, we define a subpopulation of normal faults that are kinematically related to the right-lateral strike-slip faults; these have a maximum length set by the spacing between the right-lateral faults. Fault size-frequency distributions also indicate differences between the normal and strike-slip fault populations. Overall, the normal faults have higher ratios of cumulative number to fault length than the strike-slip population does, which we relate to different patterns of localization of faulting. We interpret these trends as reflecting different tectonic histories, with the majority of normal faults being intraplate faults associated with Basin and Range extension and the strike-slip faults being kinematically connected with plate boundary.

  12. Age, tectonic evolution and origin of the Aswa Shear Zone in Uganda: Activation of an oblique ramp during convergence in the East African Orogen

    NASA Astrophysics Data System (ADS)

    Saalmann, K.; Mänttäri, I.; Nyakecho, C.; Isabirye, E.

    2016-05-01

    The Aswa Shear Zone (ASZ) is a major NW-SE trending structure of over 1000 km length in East Africa. In Uganda, the ASZ is a steeply NE-dipping, up to 11 km wide mylonitic shear zone that shows multiple stage brittle reactivation. On outcrop-scale, the fabric in the ASZ is characterized by a well-developed NW-SE striking and subvertical or steeply NE or SW dipping mylonitic foliation and a subhorizontal to moderately NW- or SE-plunging stretching lineation. Sinistral kinematics and fabric are very consistent along strike. The strain is heterogeneously distributed and partitioned into lens-shaped lower strain zones dominated by folding and characterized by pure shear, which are surrounded by high strain zones, some of them thick ultramylonites, with intense simple shear combined with flattening and strong transposition of pre-existing fabrics. Ductile shearing occurred during bulk E-W shortening, commenced at amphibolite facies conditions and continued with similar kinematics at greenschist and even lower grade conditions. A number of (sub-)parallel shear zones occur to the NE and SW of the main zone at a distance of up to 20-45 km. They show similar fabrics and kinematics and are thus related to activity along ASZ reflecting strain partitioning into simple shear and pure shear domains on a regional scale. Samples of mylonitic gneisses from the shear zone have been analyzed with U-Pb LA-MC-ICPMS and show Neoarchaean crystallisation ages between 2.66 and 2.61 Ga. Timing of ductile sinistral shearing is poorly constrained by lower intercept ages of 686 ± 62 and 640 ± 44 Ma. The fabric and structural relationship of the ca. 660 Ma Adjumani Granite exposed in the northern segment of ASZ suggest that the age of shear activity can be further limited to ca. 685 and 655 Ma. The Aswa Shear Zone is interpreted as an intra-cratonic, crustal-scale structure close to the northeastern margin of the Congo Craton, possibly inherited from previous continental extension. Early Aswa

  13. Age, tectonic evolution and origin of the Aswa Shear Zone in Uganda: Activation of an oblique ramp during convergence in the East African Orogen

    NASA Astrophysics Data System (ADS)

    Saalmann, K.; Mänttäri, I.; Nyakecho, C.; Isabirye, E.

    2016-05-01

    The Aswa Shear Zone (ASZ) is a major NW-SE trending structure of over 1000 km length in East Africa. In Uganda, the ASZ is a steeply NE-dipping, up to 11 km wide mylonitic shear zone that shows multiple stage brittle reactivation. On outcrop-scale, the fabric in the ASZ is characterized by a well-developed NW-SE striking and subvertical or steeply NE or SW dipping mylonitic foliation and a subhorizontal to moderately NW- or SE-plunging stretching lineation. Sinistral kinematics and fabric are very consistent along strike. The strain is heterogeneously distributed and partitioned into lens-shaped lower strain zones dominated by folding and characterized by pure shear, which are surrounded by high strain zones, some of them thick ultramylonites, with intense simple shear combined with flattening and strong transposition of pre-existing fabrics. Ductile shearing occurred during bulk E-W shortening, commenced at amphibolite facies conditions and continued with similar kinematics at greenschist and even lower grade conditions. A number of (sub-)parallel shear zones occur to the NE and SW of the main zone at a distance of up to 20-45 km. They show similar fabrics and kinematics and are thus related to activity along ASZ reflecting strain partitioning into simple shear and pure shear domains on a regional scale. Samples of mylonitic gneisses from the shear zone have been analyzed with U-Pb LA-MC-ICPMS and show Neoarchaean crystallisation ages between 2.66 and 2.61 Ga. Timing of ductile sinistral shearing is poorly constrained by lower intercept ages of 686 ± 62 and 640 ± 44 Ma. The fabric and structural relationship of the ca. 660 Ma Adjumani Granite exposed in the northern segment of ASZ suggest that the age of shear activity can be further limited to ca. 685 and 655 Ma. The Aswa Shear Zone is interpreted as an intra-cratonic, crustal-scale structure close to the northeastern margin of the Congo Craton, possibly inherited from previous continental extension

  14. Alpine re-activation of pre-Alpine structures: details from a large-scale shear zone in the Aar massif (Central Alps)

    NASA Astrophysics Data System (ADS)

    Wehrens, Philip; Baumberger, Roland; Herwegh, Marco

    2013-04-01

    movement towards strike-slip shearing during a late stage of the shear zone activity. This highest strain event clearly shows a 160/80° orientation of mylonitic and ultramylonitic foliations. In more competent boudins-shaped rocks open fissures developed within the high strain domain. Shear sense indicators related to the subhorizontal lineations, i.e. C' structures, sheared boudins and asymmetric folds, indicate a dextral shear sense. This dextral shearing is coeval with dextral faulting along the Simplon line and represents the third reactivation of the pre-Alpine anisotropies. In sum, this shear zone illustrates the importance of mechanical anisotropies and pre-existing structures for strain distribution, localization and shear zone kinematics in case of the basement rocks.

  15. Microstructure and rolling capability of modified AZ31-Ce-Gd alloys

    SciTech Connect

    Li Wenping; Zhou Hong; Lin Pengyu; Zhao Shizhe

    2009-11-15

    AZ31-Ce-Gd alloys were studied and the influence of cerium (Ce) and gadolinium (Gd) on the microstructure and rolling capability of AZ31 alloy was investigated. The results indicated that the grains of AZ31 alloy were refined with Ce and Gd addition. Ce and Gd addition resulted in the formation of Al{sub 4}Ce, Al{sub 2}Gd and Mg{sub 3}Gd. After homogenization and rolling, the Al{sub 4}Ce, Al{sub 2}Gd and Mg{sub 3}Gd still existed. The rolling capability of AZ31 alloy was improved obviously with Ce and Gd addition. However, once Gd content increased to a certain value, the rolling capability of the modified alloy declined but still better than that of AZ31 alloy.

  16. Assessing the previous activity at the source zone of the 2001 Bhuj earthquake based on the near-source and distant paleoseismological indicators

    NASA Astrophysics Data System (ADS)

    Rajendran, C. P.; Rajendran, Kusala; Thakkar, M.; Goyal, Bhanu

    2008-05-01

    The Mw 7.7 2001 Bhuj (Kachchh) earthquake was not associated with any primary surface rupture, but it produced secondary faulting, folding and liquefaction. This study highlights the potential of a secondary rupture and proxies like lateral spreads and sandblows in unraveling the past activity related to the 2001 source. Chronological constraints of an older lateral spread and far-field paleoliquefaction features, combined with archeological data, provide evidence for occurrences of two previous earthquakes at the 2001 source zone about 4000 and 9000 years, ago. Distinct stratigraphic evidence for at least one previous offset dated at 4424 ± 656 years could be detected at a stepover zone associated with a dextral secondary fault, reactivated during the 2001 earthquake. The studies imply longer interseismic intervals for the 2001 source zone, in comparison with the source zone of the 1819 earthquake located toward the northwestern part of the Rann of Kachchh. The spatial and temporal correlation of previous events derived on the basis of the available paleoseismic data from the region suggest not only repeated activity at the 2001 source, but possibility for additional potential sources in parts of Kachchh and Cambay basins. Although we infer a longer recurrence interval for the 2001 Bhuj earthquake source, our study points to the fact that these additional sources may have the potential to rupture in the future, considering the long elapsed time.

  17. Effects of thermomechanical treatments on the microstructures and mechanical properties of GTA-welded AZ31B magnesium alloy

    NASA Astrophysics Data System (ADS)

    Chu, Ya-jie; Chen, Jian; Li, Xiao-quan; Wu, Shen-qing; Yang, Zong-hui

    2012-10-01

    Thermomechanical treatments were carried out to improve the properties of AZ31B joints prepared by gas tungsten arc welding. The microstructures of the joints were studied by optical microscopy and scanning electron microscopy with energy-dispersive spectrometry. Tensile tests and hardness tests were performed to investigate the effects of thermomechanical treatments on the mechanical properties of the joints. It is found that the thermomechanical-treated joints show superior mechanical properties against the as-welded joints, and their ultimate tensile strength can reach more than 92% of the base material. This mainly attributes to the formation of fine equiaxed grains in the fusion zone. After thermomechanical treatments the dendrites are transformed to fine spherical grains, and the dendritic segregation can be effectively eliminated.

  18. Impact of a distance estimation error inducing a visualized zone gap on the target illuminance in range-gated active imaging.

    PubMed

    Matwyschuk, Alexis

    2014-01-01

    Some stand-alone airborne systems of target reconnaissance such as a missile seeker head use range-gated laser active imaging to visualize a target in the scene. To center the visualized zone on the target, it is important to know the distance between the active imaging system and the target. However, as this exact distance is not known before the detection of the target, it can be only estimated. This estimated distance can be erroneous (max≈500  m) with some technological drifts (gyrometric drift, accelerometric drift, missile position error, etc.). To be able to evaluate the impact of a distance estimation error on target illuminance in active imaging, the expressions of the illuminance attenuation ratio according to the decentered target position with regard to the visualized zone were determined. These different equations will be used to determine, in future stand-alone reconnaissance systems, the target signal-to-noise ratio as a function of the localization error. Generally speaking, two modes of visualization were used: first by using a fixed width of the visualized zone, and second by increasing the width of the visualized zone as a function of the distance. The defined different expressions allowed us to study the illuminance behavior of the target with regard to the value of the gap (difference between the estimated distance and the real distance) for each mode of visualization. The results showed that from a target distance of about 1 km, the visualization mode with variable zone width allowed us to decrease the target illuminance less during a gap caused by an estimation error of the target distance. PMID:24513988

  19. Biaxial Deformation of the Magnesium Alloy AZ80

    NASA Astrophysics Data System (ADS)

    Tomlinson, P.; Azizi-Alizamini, H.; Poole, W. J.; Sinclair, C. W.; Gharghouri, M. A.

    2013-07-01

    The multiaxial deformation of magnesium alloys is important for developing reliable, robust models for both the forming of components and also analysis of in-service performance of structures, for example, in the case of crash worthiness. The current study presents a combination of unique biaxial experimental tests and biaxial crystal plasticity simulations using a visco-plastic self-consistent (VPSC) formulation conducted on a relatively weak AZ80 cast texture. The experiments were conducted on tubular samples which are loaded in axial tension or compression along the tube and with internal pressure to generate hoop stresses orthogonal to the axial direction. The results were analyzed in stress and strain space and also in terms of the evolution of crystallographic texture. In general, it was found that the VPSC simulations matched well with the experiments. However, some differences were observed for cases where basal slip and \\{ {10bar{1}2} \\} extension twinning were in close competition such as in the biaxial tension quadrant of the plastic potential. The evolution of texture measured experimentally and predicted from the VPSC simulations was qualitatively in good agreement. Finally, experiments and VPSC simulations were conducted on a second AZ80 material which had a stronger initial texture and a higher level of mechanical anisotropy. In the previous case, the agreement between experiments and simulations was good, but a larger difference was observed in the biaxial tension quadrant of the plastic potential.

  20. Selection of a suitable plant for phytoremediation in mining artisanal zones.

    PubMed

    Chamba, I; Gazquez, M J; Selvaraj, T; Calva, J; Toledo, J J; Armijos, C

    2016-09-01

    A study was undertaken with the aim of identifying a suitable plant for the phytoremediation of metal-polluted soil from an artisanal mining area in Ecuador. Three zones including a natural zone (NZ), abandoned zone (AZ) and intensively mined zone (IZ) were selected. Three common native plants grown in the three zones were identified and collected, including Miconia zamorensis, Axonopus compressus and Erato polymnioides. The percentage of arbuscular mycorrhizal colonization that benefits their own survival in polluted soil was analyzed in the root samples of these candidate species. Analysis of the soils and plants collected from the different zones showed that the concentrations of Pb, Zn, Cu and Cd were comparatively lower in the NZ, higher in the AZ and IZ, and highest in the AZ for all the metals. The concentration of all these metals in plant tissues was the highest in E. polymnioides. The data analysis including the metal accumulation index, bioconcentration factor and translocation factor strongly identified E. polymnioides as a hyperaccumulator plant suitable for phytoremediation. PMID:26940037

  1. Characteristics of the Colombian armed conflict and the mental health of civilians living in active conflict zones

    PubMed Central

    2012-01-01

    Background Despite the fact that the Colombian armed conflict has continued for almost five decades there is still very little information on how it affects the mental health of civilians. Although it is well established in post-conflict populations that experience of organised violence has a negative impact on mental health, little research has been done on those living in active conflict zones. Médecins Sans Frontières provides mental health services in areas of active conflict in Colombia and using data from these services we aimed to establish which characteristics of the conflict are most associated with specific symptoms of mental ill health. Methods An analysis of clinical data from patients (N = 6,353), 16 years and over, from 2010–2011, who consulted in the Colombian departments (equivalent to states) of Nariño, Cauca, Putumayo and Caquetá. Risk factors were grouped using a hierarchical cluster analysis and the clusters were included with demographic information as predictors in logistic regressions to discern which risk factor clusters best predicted specific symptoms. Results Three clear risk factor clusters emerged which were interpreted as ‘direct conflict related violence’, ‘personal violence not directly conflict-related’ and ‘general hardship’. The regression analyses indicated that conflict related violence was more highly related to anxiety-related psychopathology than other risk factor groupings while non-conflict violence was more related to aggression and substance abuse, which was more common in males. Depression and suicide risk were represented equally across risk factor clusters. Conclusions As the largest study of its kind in Colombia it demonstrates a clear impact of the conflict on mental health. Among those who consulted with mental health professionals, specific conflict characteristics could predict symptom profiles. However, some of the highest risk outcomes, like depression, suicide risk and aggression, were more

  2. Cycling of Dissolved Organic Phosphorus and Alkaline Phosphatase Activity in Euphotic Zone of the Western North Pacific

    NASA Astrophysics Data System (ADS)

    Suzumura, M.

    2010-12-01

    Phosphorus is an essential nutrient for marine organisms. In oligotrophic environments, concentrations of dissolved inorganic phosphate (SRP), the most bioavailable form of phosphorus, are low and have been hypothesized to constrain the primary productivity. Evidence has been found that dissolved organic phosphorus (DOP) supports a significant fraction of primary production through hydrolytic remineralization of DOP to SRP by alkaline phosphatase (APA). In this study, DOP biogeochemistry was investigated at three locations of the open-ocean environment in the Kuroshio region and at a semi-eutrophic coastal site of the western North Pacific. Concentrations of SRP, DOP and hydrolyzable ester-P were measured in the euphotic zone. Kinetic parameters of APA were determined using a fluorogenic substrate, including potential maximum velocity (Vmax), apparent Michaelis-Menten half-saturation constant (Km), and turnover time (TA) of APA hydrolyzable DOP. SRP concentrations were quite low (≤ 10 nM) in the surface seawater and rapidly increased below the chlorophyll a maximum layer (CML). DOP concentration ranged from 29 to 223 nM. Above the CML, DOP composed a major fraction accounting for 60-100% of dissolved total P. A significant linear relationship was found between the concentrations of SRP and hydrolyzable ester-P (R2 = 0.83, P < 0.01). This suggests active utilization of ester-P under phosphate-depleted conditions. In the Kuroshio region, Vmax of APA exhibited the highest value at the surface water (0 m) and decreased rapidly with depth, while at the coastal site the peak value was found at CML. TA of hydrolyzable DOP was quite variable among the locations and increased with depth especially below CML. The estimated values of in situ hydrolysis rate were much lower (2-34%) than the potential Vmax which was determined with the addition of an excess amount of the substrate. The results suggest that marine microbes can efficiently and rapidly utilize hydrolyzable DOP

  3. Active Deformation in the Greater Himalayan Zone in Western Nepal from Inversion of New (U-Th)/He Cooling Ages

    NASA Astrophysics Data System (ADS)

    Harvey, J. E.; Burbank, D.

    2015-12-01

    Much of the central Himalaya features an abrupt rise in mean elevation from ~1.5 km in the Lesser Himalaya to ~4-5 km Greater Himalaya and Tibetan Plateau. This physiographic transition is known as PT2, and is often interpreted as the surface expression of transport over a ramp in the Main Himalayan Thrust (MHT). In western Nepal, however, the same rise in elevation occurs over two distinct topographic steps (PT2-N and PT2-S). In previous work, Harvey et al. (2015) argue that this anomalous topography is the result of recent southward-migration of mid-crustal deformation along the MHT. Due to the seismogenic potential of the MHT it is important to constrain its geometry in the western Nepal seismic gap, which has not had a large earthquake in over 600 years. To test the above hypothesis, we perform [U-Th]/He dating on 39 apatite and 47 zircon samples collected along seven relief transects throughout western Nepal. We constrain exhumation histories by inverting these new cooling ages with the 3-D thermo-kinematic model Pecube. Five transects collected from the Greater Himalaya north of PT2-N are best fit by relatively rapid exhumation rates (~1-2 km/Myr) since ~4 Ma. The other two, collected from farther south near PT2-S, require rapid (~1-2 km/Myr) exhumation until around 8-11 Ma, followed by much slower (~0.1-0.2 km/Myr) exhumation until at least the late Pliocene. Assuming that exhumation rates reflect uplift rates, the rapid Plio-Pleistocene exhumation in the Greater Himalaya north of PT2-N suggest that this physiographic transition is similar to that at the foot of the Greater Himalaya in central Nepal. It follows that active deformation is occurring along a NW-trend as much as 100 km farther north than would be expected if simply projecting PT2 across western Nepal. This finding is consistent with transport over a more northerly MHT ramp or perhaps oblique slip along the recently identified, surface-breaking WNFZ. Although the geomorphology and microseismicity

  4. Comment on "A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme".

    PubMed

    Wang, Feng; Ranjan, Anand; Wei, Debbie; Wu, Carl

    2016-07-22

    Watanabe et al (Reports, 12 April 2013, p. 195) study the yeast SWR1/SWR-C complex responsible for depositing the histone variant H2A.Z by replacing nucleosomal H2A with H2A.Z. They report that reversal of H2A.Z replacement is mediated by SWR1 and related INO80 on an H2A.Z nucleosome carrying H3K56Q. Using multiple assays and reaction conditions, we find no evidence of such reversal of H2A.Z exchange. PMID:27463665

  5. Demonstration of variation in chondrocyte activity in different zones of articular cartilage: an assessment of the value of in-situ hybridization.

    PubMed Central

    Marles, P. J.; Hoyland, J. A.; Parkinson, R.; Freemont, A. J.

    1991-01-01

    Several methods have been described for investigating chondrocyte metabolism in vitro. In this study, in-situ hybridization (ISH) using an oligonucleotide probe (i.e. a poly-d(T) probe) to detect total messenger RNA (mRNA) in cartilage explants has been compared with radiosulphate and radioleucine uptake studies in an attempt to assess the value of ISH in investigating chondrocyte metabolism. The relative results of the three parameters indicate qualitative similarities in cells in the intermediate, deep and calcified zones but differences in the superficial zone. The relative levels of mRNA and leucine and sulphate uptake in the midzone areas could be construed as indicating that the bulk of cellular activity was directed towards the synthesis of proteoglycans. A similar relation between the three parameters, but at a lower level, was seen in chondrocytes in the calcified zone demonstrating that these cells are viable and biosynthetic. Both quantitative and qualitative differences between the three methods were observed in the superficial chondrocytes regarding the amount of mRNA compared to sulphate and leucine uptake. The results suggest that ISH can detect differences in the amount of mRNA present in chondrocytes in differing zones of cartilage and, like the radioleucine and radiosulphate studies, particularly emphasizes their functional heterogeneity. Images Fig. 4 Fig. 6 PMID:2015200

  6. 78 FR 68026 - Foreign-Trade Zone 32-Miami, Florida, Authorization of Production Activity, Almod Diamonds, Ltd...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... 400), including notice in the Federal Register inviting public comment (78 FR 40427, 7-5-2013). The..., Almod Diamonds, Ltd. (Jewelry and Precious Stones), Miami, Florida On June 21, 2013, the Greater Miami... the Foreign-Trade Zones (FTZ) Board on behalf of Almod Diamonds, Ltd., within FTZ 32--Site 1, in...

  7. 15 CFR 930.98 - Federally assisted activities outside of the coastal zone or the described geographic area.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE OCEAN AND COASTAL RESOURCE MANAGEMENT FEDERAL CONSISTENCY WITH APPROVED COASTAL MANAGEMENT PROGRAMS Consistency for Federal Assistance to State and Local Governments § 930.98... of the coastal zone or the described geographic area. 930.98 Section 930.98 Commerce and...

  8. The Mediation of Learning in the Zone of Proximal Development through a Co-Constructed Writing Activity

    ERIC Educational Resources Information Center

    Thompson, Ian

    2013-01-01

    This article develops a theoretical understanding of the processes involved in the co-construction of a written text by a teacher and student from a Vygotskian perspective. Drawing on cultural-historical and sociocultural theories of writing and Vygotsky's concept of the Zone of Proximal Development (ZPD), this case study of a student and…

  9. Crystal structures of heterotypic nucleosomes containing histones H2A.Z and H2A

    PubMed Central

    Horikoshi, Naoki; Arimura, Yasuhiro; Taguchi, Hiroyuki; Kurumizaka, Hitoshi

    2016-01-01

    H2A.Z is incorporated into nucleosomes located around transcription start sites and functions as an epigenetic regulator for the transcription of certain genes. During transcriptional regulation, the heterotypic H2A.Z/H2A nucleosome containing one each of H2A.Z and H2A is formed. However, previous homotypic H2A.Z nucleosome structures suggested that the L1 loop region of H2A.Z would sterically clash with the corresponding region of canonical H2A in the heterotypic nucleosome. To resolve this issue, we determined the crystal structures of heterotypic H2A.Z/H2A nucleosomes. In the H2A.Z/H2A nucleosome structure, the H2A.Z L1 loop structure was drastically altered without any structural changes of the canonical H2A L1 loop, thus avoiding the steric clash. Unexpectedly, the heterotypic H2A.Z/H2A nucleosome is more stable than the homotypic H2A.Z nucleosome. These data suggested that the flexible character of the H2A.Z L1 loop plays an essential role in forming the stable heterotypic H2A.Z/H2A nucleosome. PMID:27358293

  10. Corrosion resistance of biodegradable polymeric layer-by-layer coatings on magnesium alloy AZ31

    NASA Astrophysics Data System (ADS)

    Cui, Lan-Yue; Zeng, Rong-Chang; Zhu, Xiao-Xiao; Pang, Ting-Ting; Li, Shuo-Qi; Zhang, Fen

    2016-03-01

    Biocompatible polyelectrolyte multilayers (PEMs) and polysiloxane hybrid coatings were prepared to improve the corrosion resistance of biodegradable Mg alloy AZ31. The PEMs, which contained alternating poly(sodium 4-styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH), were first self-assembled on the surface of the AZ31 alloy substrate via electrostatic interactions, designated as (PAH/PSS)5/AZ31. Then, the (PAH/PSS)5/AZ31 samples were dipped into a methyltrimethoxysilane (MTMS) solution to fabricate the PMTMS films, designated as PMTMS/(PAH/PSS)5/AZ31. The surface morphologies, microstructures and chemical compositions of the films were investigated by FE-SEM, FTIR, XRD and XPS. Potentiodynamic polarization, electrochemical impedance spectroscopy and hydrogen evolution measurements demonstrated that the PMTMS/(PAH/PSS)5/AZ31 composite film significantly enhanced the corrosion resistance of the AZ31 alloy in Hank's balanced salt solution (HBSS). The PAH and PSS films effectively improved the deposition of Ca-P compounds including Ca3(PO4)2 and hydroxyapatite (HA). Moreover, the corrosion mechanism of the composite coating was discussed. These coatings could be an alternative candidate coating for biodegradable Mg alloys.

  11. Corrosion resistance of biodegradable polymeric layer-by-layer coatings on magnesium alloy AZ31

    NASA Astrophysics Data System (ADS)

    Cui, Lan-Yue; Zeng, Rong-Chang; Zhu, Xiao-Xiao; Pang, Ting-Ting; Li, Shuo-Qi; Zhang, Fen

    2016-06-01

    Biocompatible polyelectrolyte multilayers (PEMs) and polysiloxane hybrid coatings were prepared to improve the corrosion resistance of biodegradable Mg alloy AZ31. The PEMs, which contained alternating poly(sodium 4-styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH), were first self-assembled on the surface of the AZ31 alloy substrate via electrostatic interactions, designated as (PAH/PSS)5/AZ31. Then, the (PAH/PSS)5/AZ31 samples were dipped into a methyltrimethoxysilane (MTMS) solution to fabricate the PMTMS films, designated as PMTMS/(PAH/PSS)5/AZ31. The surface morphologies, microstructures and chemical compositions of the films were investigated by FE-SEM, FTIR, XRD and XPS. Potentiodynamic polarization, electrochemical impedance spectroscopy and hydrogen evolution measurements demonstrated that the PMTMS/(PAH/PSS)5/AZ31 composite film significantly enhanced the corrosion resistance of the AZ31 alloy in Hank's balanced salt solution (HBSS). The PAH and PSS films effectively improved the deposition of Ca-P compounds including Ca3(PO4)2 and hydroxyapatite (HA). Moreover, the corrosion mechanism of the composite coating was discussed. These coatings could be an alternative candidate coating for biodegradable Mg alloys.

  12. Microstructure and Elevated Temperature Properties of Die-cast AZ91- xNd Magnesium Alloys

    NASA Astrophysics Data System (ADS)

    Wang, Jun; Wang, Limin; An, Jian; Liu, Yongbing

    2008-10-01

    The effect of Nd addition on the microstructure and mechanical properties of a die-cast AZ91 alloy was investigated in the present work. The results show that the die-cast AZ91 alloy is composed of α-Mg matrix and γ-Mg17Al12 phase. Nd addition into the AZ91 alloy leads to the formation of rare earth containing intermetallic phase. Al4Nd phase forms when Nd content is less than or equal to 1.0 wt.%. Al2Nd phase appears simultaneously when Nd content reaches to 3.0 wt.%. The size and volume fraction of γ-Mg17Al12 phase decrease, because of the newly formed Al-Nd phase. And the γ-Mg17Al12 phase distributes from reticular to dispersive. Nd addition has a little effect on the room temperature properties of the die-cast AZ91 alloy, but greatly improves the elevated temperature properties. The tensile strength of AZ91-0.5Nd and AZ91-1.0Nd alloy tested at 150 °C is even close to the room temperature strength. The AZ91-1.0Nd alloy has the optimal properties.

  13. Effect of Annealing on Microstructure and Tensile Properties of 5052/AZ31/5052 Clad Sheets

    NASA Astrophysics Data System (ADS)

    Nie, Huihui; Liang, Wei; Chi, Chengzhong; Li, Xianrong; Fan, Haiwei; Yang, Fuqian

    2016-05-01

    Three-layered 5052Al/AZ31Mg/5052Al (5052/AZ31/5052) clad sheets were fabricated by four-pass rolling and annealed under different conditions. Under the optimal annealing condition, homogeneous and equiaxial grains with an average AZ31 grain size of 5.24 µm were obtained and the maximum values of ultimate tensile strength and elongation of the clad sheet reached 230 MPa and 18%, respectively. Electron backscatter diffraction analysis showed that the AZ31 layer had a typical rolling texture with its c-axis parallel to the normal direction. The fraction of low-angle grain boundaries in the 5052 layer was nearly four times more than that in the AZ31 layer because of different deformation extent and recrystallization driving forces. The textures of Al3Mg2 and Mg17Al12 were similar to that of 5052 because of the deformation coordination during the rolling and recrystallization process. The orientation relationship between Mg17Al12 and AZ31 seemed to be (110) Mg17Al12//(10-11) AZ31.

  14. Iron Phosphate Glass for Vitrifying Hanford AZ102 LAW in Joule Heated and Cold Crucible Induction Melters - 12240

    SciTech Connect

    Day, Delbert E.; Brow, Richard K.; Ray, Chandra S.; Reis, Signo T.; Kim, Cheol-Woon; Vienna, John D.; Sevigny, Gary; Peeler, David; Johnson, Fabienne C.; Hansen, Eric K.; Soelberg, Nick; Pegg, Ian L.; Gan, Hao

    2012-07-01

    An iron phosphate composition for vitrifying a high sulfate (∼17 wt%) and high alkali (∼80 wt%) Hanford low activity waste (LAW), known as AZ-102 LAW, has been developed for processing in a Joule Heated Melter (JHM) or a Cold Crucible Induction Melter (CCIM). This composition produced a glass waste form, designated as MS26AZ102F-2, with a waste loading of 26 wt% of the AZ-102 which corresponded to a total alkali and sulfate (represented as SO{sub 3}) content of 21 and 4.4 wt%, respectively. A slurry (7 M Na{sup +}) of MS26AZ102F-2 simulant was melted continuously at temperatures between 1030 and 1090 deg. C for 10 days in a small JHM at PNNL and for 70 hours in a CCIM at INL. The as-cast glasses produced in both melters and in trial laboratory experiments along with their canister centerline cooled (CCC) counterparts met the requirements for the Product Consistency Test (PCT) and the Vapor Hydration Test (VHT) responses in the Hanford Tank Waste Treatment and Immobilization Plant (WTP) Contract. These glass waste forms retained up to 77 % of the SO{sub 3} (3.3 wt%), 100% of the Cesium, and 33 to 44% of the rhenium (used as a surrogate for Tc) all of which either exceeded or were comparable to the retention limit for these species in borosilicate glass nuclear waste form. Analyses of commercial K-3 refractory lining and the Inconel 693 metal electrodes used in JHM indicated only minimum corrosion of these components by the iron phosphate glass. This is the first time that an iron phosphate composition was melted continuously in a slurry fed JHM and in the US, thereby, demonstrating that iron phosphate glasses can be used as alternative hosts for vitrifying nuclear waste. The following conclusions are drawn from the results of the present work. (1) An iron phosphate composition, designated as MS26AZ102F-2, containing 26 wt% of the simulated high sulfate (17 wt%), high alkali (80 wt%) Hanford AZ-102 LAW meets all the criteria for processing in a JHM and CCIM. This

  15. Conformal AZ5214-E resist deposition on patterned (1 0 0) InP substrates

    NASA Astrophysics Data System (ADS)

    Eliás, P.; Gregusová, D.; Martaus, J.; Kostic, I.

    2006-02-01

    A draping technique was studied to deposit thin, visco-elastic AZ5214-E resist layers from a water surface over planar and patterned substrates. A visco-elastic AZ5214-E layer forms on the water surface when a drop of AZ5214-E makes contact with it. The drop spreads out within a fraction of a second because of a large positive difference in surface tension between water and AZ5214-E. The spreading mechanism lies in the ability of PGMEA (AZ5214-E liquid constituent) to form hydrogen bonds with water. We brought AZ5214-E drops to make contact with water at 20 ± 0.1 °C via adhesive forces to form AZ5214-E layers on the water surface of (1) circular and (2) square shapes. In case (1), the layers, formed from drops of V = 3 µl, had thickness t ap c sdot 4V/πΦ-2 for Φ < 32 mm, and t > c sdot 4V/πΦ-2 for larger Φ. In case (2), the layers had t ap a + bV for V between 7 and 12 µl on square-shaped water surfaces of constant area A = 34.5 mm × 34.5 mm. All layers exhibited microscopic waviness with an average thickness uniformity u ~ 91%, and submicron waviness with a root-mean-square roughness σ ~ 12 nm and a lateral correlation length ξ ~ 32 µm. AZ5214-E sheets coated conformally high mesa objects with sharp convex and concave edges: 61 µm high ridges confined to ~35°-inclined facets and 9 µm high ridges confined to negatively sloped facets. The draping technique can be used to deposit conformal AZ5214-E layers over non-planar substrates for non-planar device processing.

  16. Stamen abscission zone transcriptome profiling reveals new candidates for abscission control: enhanced retention of floral organs in transgenic plants overexpressing Arabidopsis ZINC FINGER PROTEIN2.

    PubMed

    Cai, Suqin; Lashbrook, Coralie C

    2008-03-01

    Organ detachment requires cell separation within abscission zones (AZs). Physiological studies have established that ethylene and auxin contribute to cell separation control. Genetic analyses of abscission mutants have defined ethylene-independent detachment regulators. Functional genomic strategies leading to global understandings of abscission have awaited methods for isolating AZ cells of low abundance and very small size. Here, we couple laser capture microdissection of Arabidopsis thaliana stamen AZs and GeneChip profiling to reveal the AZ transcriptome responding to a developmental shedding cue. Analyses focus on 551 AZ genes (AZ(551)) regulated at the highest statistical significance (P < or = 0.0001) over five floral stages linking prepollination to stamen shed. AZ(551) includes mediators of ethylene and auxin signaling as well as receptor-like kinases and extracellular ligands thought to act independent of ethylene. We hypothesized that novel abscission regulators might reside in disproportionately represented Gene Ontology Consortium functional categories for cell wall modifying proteins, extracellular regulators, and nuclear-residing transcription factors. Promoter-beta-glucuronidase expression of one transcription factor candidate, ZINC FINGER PROTEIN2 (AtZFP2), was elevated in stamen, petal, and sepal AZs. Flower parts of transgenic lines overexpressing AtZFP2 exhibited asynchronous and delayed abscission. Abscission defects were accompanied by altered floral morphology limiting pollination and fertility. Hand-pollination restored transgenic fruit development but not the rapid abscission seen in wild-type plants, demonstrating that pollination does not assure normal rates of detachment. In wild-type stamen AZs, AtZFP2 is significantly up-regulated postanthesis. Phenotype data from transgene overexpression studies suggest that AtZFP2 participates in processes that directly or indirectly influence organ shed. PMID:18192438

  17. Radiant zone heated particulate filter

    DOEpatents

    Gonze, Eugene V [Pinckney, MI

    2011-12-27

    A system includes a particulate matter (PM) filter including an upstream end for receiving exhaust gas and a downstream end. A radiant zoned heater includes N zones, where N is an integer greater than one, wherein each of the N zones includes M sub-zones, where M is an integer greater than or equal to one. A control module selectively activates at least a selected one of the N zones to initiate regeneration in downstream portions of the PM filter from the one of the N zones, restricts exhaust gas flow in a portion of the PM filter that corresponds to the selected one of the N zones, and deactivates non-selected ones of the N zones.

  18. More Docked Vesicles and Larger Active Zones at Basket Cell-to-Granule Cell Synapses in a Rat Model of Temporal Lobe Epilepsy

    PubMed Central

    Yamawaki, Ruth; Thind, Khushdev

    2016-01-01

    Temporal lobe epilepsy is a common and challenging clinical problem, and its pathophysiological mechanisms remain unclear. One possibility is insufficient inhibition in the hippocampal formation where seizures tend to initiate. Normally, hippocampal basket cells provide strong and reliable synaptic inhibition at principal cell somata. In a rat model of temporal lobe epilepsy, basket cell-to-granule cell (BC→GC) synaptic transmission is more likely to fail, but the underlying cause is unknown. At some synapses, probability of release correlates with bouton size, active zone area, and number of docked vesicles. The present study tested the hypothesis that impaired GABAergic transmission at BC→GC synapses is attributable to ultrastructural changes. Boutons making axosomatic symmetric synapses in the granule cell layer were reconstructed from serial electron micrographs. BC→GC boutons were predicted to be smaller in volume, have fewer and smaller active zones, and contain fewer vesicles, including fewer docked vesicles. Results revealed the opposite. Compared with controls, epileptic pilocarpine-treated rats displayed boutons with over twice the average volume, active zone area, total vesicles, and docked vesicles and with more vesicles closer to active zones. Larger active zones in epileptic rats are consistent with previous reports of larger amplitude miniature IPSCs and larger BC→GC quantal size. Results of this study indicate that transmission failures at BC→GC synapses in epileptic pilocarpine-treated rats are not attributable to smaller boutons or fewer docked vesicles. Instead, processes following vesicle docking, including priming, Ca2+ entry, or Ca2+ coupling with exocytosis, might be responsible. SIGNIFICANCE STATEMENT One in 26 people develops epilepsy, and temporal lobe epilepsy is a common form. Up to one-third of patients are resistant to currently available treatments. This study tested a potential underlying mechanism for previously reported

  19. Comparative analysis of geodynamic activity of the Caucasian and Eastern Mediterranean segments of the Alpine-Himalayan convergence zone

    NASA Astrophysics Data System (ADS)

    Chelidze, Tamaz; Eppelbaum, Lev

    2013-04-01

    The Alpine-Himalayan convergence zone (AHCZ) underwent recent transverse shortening under the effect of collisional compression. The process was accompanied by rotation of separate microplates. The Caucasian and Eastern Mediterranean regions are segments of the of the AHCZ and are characterized by intensive endogenous and exogenous geodynamic processes, which manifest themselves in occurrence of powerful (with magnitude of 8-9) earthquakes accompanied by development of secondary catastrophic processes. Large landslides, rock falls, avalanches, mud flows, etc. cause human deaths and great material losses. The development of the aforesaid endogenous processes is set forth by peculiarities of the deep structure of the region and an impact of deep geological processes. The Caucasus is divided into several main tectonic terranes: platform (sub-platform, quasi-platform) and fold-thrust units. Existing data enable to perform a division of the Caucasian region into two large-scale geological provinces: southern Tethyan and northern Tethyan located to the south of and to the north of the Lesser Caucasian ophiolite suture, respectively. The recent investigations show that the assessments of the seismic hazard in these regions are not quite correct - for example in the West Caucasus the seismic hazard can be significantly underestimated, which affects the corresponding risk assessments. Integrated analysis of gravity, magnetic, seismic and thermal data enables to refine the assessment of the seismic hazard of the region, taking into account real rates of the geodynamic movements. Important role play the last rheological constructions. According to Reilinger et al. (2006) tectonic scheme, the West flanking of the Arabian Plate manifests strike-slip motion, when the East Caucasian block is converging and shortening. The Eastern Mediterranean is a tectonically complex region located in the midst of the progressive Afro-Eurasian collision. The recent increasing geotectonic

  20. An 18-Inch Direct Drive Alt-Az Telescope

    NASA Astrophysics Data System (ADS)

    Genet, Russell M.; Rowe, Dave; Gray, Dan; Albertson, Billy; Chiu, Wilson; Kirkup, Michelle; Murphy, Drew; Ridgely, John; Schmitt, Josh; Swanson, Matt; Urban, Rob; Liu, Tong; Branch, Howard; Chelborad, Cary; Keller, Allan

    2008-05-01

    A modest aperture alt-az telescope has been designed and is being built for student use at California Polytechnic State University. The telescope's drive system has no gears, belts, or friction wheels; instead direct drive motors and high resolution encoders are completely integrated into the bearing assemblies and telescope superstructure. In altitude, for instance, a ring of permanent magnets is firmly mounted to the OTA while an opposing ring of coils is mounted on the inside of a fork arm. The electronic control system has been designed to operate these brushless motors in a high- precision mode. To achieve the highest possible closed loop servo bandwidth, the structure was designed -- using finite element analysis as well as traditional tools -- to have a very high natural frequency. The direct drive system and stiff structure should effectively counter wind gusts when the telescope is operated out in the open or within a roll-off roof observatory.