Is there a role for anterior zone sampling as part of saturation trans-rectal ultrasound guided prostate biopsy?

PubMed

Cole, Eric; Margel, David; Greenspan, Michael; Shayegan, Bobby; Matsumoto, Edward; Fischer, Marc A; Patlas, Michael; Daya, Dean; Pinthus, Jehonathan H

2014-05-03

The prostatic anterior zone (AZ) is not targeted routinely by TRUS guided prostate biopsy (TRUS-Pbx). MRI is an accurate diagnostic tool for AZ tumors, but is often unavailable due to cost or system restrictions. We examined the diagnostic yield of office based AZ TRUS-Pbx. 127 men at risk for AZ tumors were studied: Patients with elevated PSA and previous extended negative TRUS-Pbx (group 1, n = 78) and actively surveyed low risk prostate cancer patients (group 2, n = 49). None of the participants had a previous AZ biopsy. Biopsy template included suspicious ultrasonic areas, 16 peripheral zone (PZ), 4 transitional zone (TZ) and 6 AZ cores. All biopsies were performed by a single urologist under local peri-prostatic anaesthetic, using the B-K Medical US System, an end-firing probe 4-12 MHZ and 18 ga/25 cm needle. All samples were reviewed by a single specialized uro-pathologist. Multivariate analysis was used to detect predictors for AZ tumors accounting for age, PSA, PSA density, prostate volume, BMI, and number of previous biopsies. Median PSA was 10.4 (group 1) and 7.3 (group 2). Age (63.9, 64.5), number of previous biopsies (1.5) and cores (17.8, 21.3) and prostate volume (56.4 cc, 51 cc) were similar for both groups. The overall diagnostic yield was 34.6% (group 1) and 85.7% (group 2). AZ cancers were detected in 21.8% (group 1) and 34.7% (group 2) but were rarely the only zone involved (1.3% and 4.1% respectively). Gleason ≥ 7 AZ cancers were often accompanied by equal grade PZ tumors. In multivariate analysis only prostate volume predicted for AZ tumors. Patients detected with AZ tumors had significantly smaller prostates (36.9 cc vs. 61.1 cc p < 0.001). Suspicious AZ ultrasonic findings were uncommon (6.3%). TRUS-Pbx AZ sampling rarely improves the diagnostic yield of extended PZ sampling in patients with elevated PSA and previous negative biopsies. In low risk prostate cancer patients who are followed by active surveillance, AZ sampling changes risk

Spermidine Suppresses Age-Associated Memory Impairment by Preventing Adverse Increase of Presynaptic Active Zone Size and Release

PubMed Central

Gupta, Varun K.; Pech, Ulrike; Fulterer, Andreas; Ender, Anatoli; Mauermann, Stephan F.; Andlauer, Till F. M.; Beuschel, Christine; Thriene, Kerstin; Quentin, Christine; Schwärzel, Martin; Mielke, Thorsten; Madeo, Frank; Dengjel, Joern; Fiala, André; Sigrist, Stephan J.

2016-01-01

Memories are assumed to be formed by sets of synapses changing their structural or functional performance. The efficacy of forming new memories declines with advancing age, but the synaptic changes underlying age-induced memory impairment remain poorly understood. Recently, we found spermidine feeding to specifically suppress age-dependent impairments in forming olfactory memories, providing a mean to search for synaptic changes involved in age-dependent memory impairment. Here, we show that a specific synaptic compartment, the presynaptic active zone (AZ), increases the size of its ultrastructural elaboration and releases significantly more synaptic vesicles with advancing age. These age-induced AZ changes, however, were fully suppressed by spermidine feeding. A genetically enforced enlargement of AZ scaffolds (four gene-copies of BRP) impaired memory formation in young animals. Thus, in the Drosophila nervous system, aging AZs seem to steer towards the upper limit of their operational range, limiting synaptic plasticity and contributing to impairment of memory formation. Spermidine feeding suppresses age-dependent memory impairment by counteracting these age-dependent changes directly at the synapse. PMID:27684064

Effect of Friction Stir Processing on Microstructure and Mechanical Properties of AZ91C Magnesium Cast Alloy Weld Zone

NASA Astrophysics Data System (ADS)

Hassani, Behzad; Karimzadeh, Fathallah; Enayati, Mohammad Hossein; Sabooni, Soheil; Vallant, Rudolf

2016-07-01

In this study, friction stir processing (FSP) was applied to the GTAW (TIG)-welded AZ91C cast alloy to refine the microstructure and optimize the mechanical properties of the weld zone. Microstructural investigation of the samples was performed by optical microscopy and the phases in the microstructure were determined by x-ray diffraction (XRD). The microstructural evaluations showed that FSP destroys the coarse dendritic microstructure. Furthermore, it dissolves the secondary hard and brittle β-Mg17Al12 phase existing at grain boundaries of the TIG weld zone. The closure and decrease in amount of porosities along with the elimination of the cracks in the microstructure were observed. These changes were followed by a significant grain refinement to an average value of 11 µm. The results showed that the hardness values increased to the mean ones, respectively, for as-cast (63 Hv), TIG weld zone (67 Hv), and stir zone (79 Hv). The yield and ultimate strength were significantly enhanced after FSP. The fractography evaluations, by scanning electron microscopy (SEM), indicated to a transition from brittle to ductile fracture surface after applying FSP to the TIG weld zone.

Characteristics of AZ31 Mg alloy joint using automatic TIG welding

NASA Astrophysics Data System (ADS)

Liu, Hong-tao; Zhou, Ji-xue; Zhao, Dong-qing; Liu, Yun-teng; Wu, Jian-hua; Yang, Yuan-sheng; Ma, Bai-chang; Zhuang, Hai-hua

2017-01-01

The automatic tungsten-inert gas welding (ATIGW) of AZ31 Mg alloys was performed using a six-axis robot. The evolution of the microstructure and texture of the AZ31 auto-welded joints was studied by optical microscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and electron backscatter diffraction. The ATIGW process resulted in coarse recrystallized grains in the heat affected zone (HAZ) and epitaxial growth of columnar grains in the fusion zone (FZ). Substantial changes of texture between the base material (BM) and the FZ were detected. The {0002} basal plane in the BM was largely parallel to the sheet rolling plane, whereas the c-axis of the crystal lattice in the FZ inclined approximately 25° with respect to the welding direction. The maximum pole density increased from 9.45 in the BM to 12.9 in the FZ. The microhardness distribution, tensile properties, and fracture features of the AZ31 auto-welded joints were also investigated.

Whiteriver Sewage Lagoons, Whiteriver, AZ: AZ0024058

EPA Pesticide Factsheets

Authorization to Discharge Under National Pollutant Discharge Elimination System (NPDES) Permit No. AZ0024058 for Tribal Utility Authority, White Mountain Apache Tribe Whiteriver Sewage Lagoons, Whiteriver, AZ.

Selection of a suitable plant for phytoremediation in mining artisanal zones.

PubMed

Chamba, I; Gazquez, M J; Selvaraj, T; Calva, J; Toledo, J J; Armijos, C

2016-09-01

A study was undertaken with the aim of identifying a suitable plant for the phytoremediation of metal-polluted soil from an artisanal mining area in Ecuador. Three zones including a natural zone (NZ), abandoned zone (AZ) and intensively mined zone (IZ) were selected. Three common native plants grown in the three zones were identified and collected, including Miconia zamorensis, Axonopus compressus and Erato polymnioides. The percentage of arbuscular mycorrhizal colonization that benefits their own survival in polluted soil was analyzed in the root samples of these candidate species. Analysis of the soils and plants collected from the different zones showed that the concentrations of Pb, Zn, Cu and Cd were comparatively lower in the NZ, higher in the AZ and IZ, and highest in the AZ for all the metals. The concentration of all these metals in plant tissues was the highest in E. polymnioides. The data analysis including the metal accumulation index, bioconcentration factor and translocation factor strongly identified E. polymnioides as a hyperaccumulator plant suitable for phytoremediation.

Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone

PubMed Central

Gundelfinger, Eckart D.; Reissner, Carsten; Garner, Craig C.

2016-01-01

Bassoon and Piccolo are two very large scaffolding proteins of the cytomatrix assembled at the active zone (CAZ) where neurotransmitter is released. They share regions of high sequence similarity distributed along their entire length and seem to share both overlapping and distinct functions in organizing the CAZ. Here, we survey our present knowledge on protein-protein interactions and recent progress in understanding of molecular functions of these two giant proteins. These include roles in the assembly of active zones (AZ), the localization of voltage-gated Ca2+ channels (VGCCs) in the vicinity of release sites, synaptic vesicle (SV) priming and in the case of Piccolo, a role in the dynamic assembly of the actin cytoskeleton. Piccolo and Bassoon are also important for the maintenance of presynaptic structure and function, as well as for the assembly of CAZ specializations such as synaptic ribbons. Recent findings suggest that they are also involved in the regulation activity-dependent communication between presynaptic boutons and the neuronal nucleus. Together these observations suggest that Bassoon and Piccolo use their modular structure to organize super-molecular complexes essential for various aspects of presynaptic function. PMID:26793095

Characterization of the stability and folding of H2A.Z chromatin particles: implications for transcriptional activation.

PubMed

Abbott, D W; Ivanova, V S; Wang, X; Bonner, W M; Ausió, J

2001-11-09

H2A.Z and H2A.1 nucleosome core particles and oligonucleosome arrays were obtained using recombinant versions of these histones and a native histone H2B/H3/H4 complement reconstituted onto appropriate DNA templates. Analysis of the reconstituted nucleosome core particles using native polyacrylamide gel electrophoresis and DNase I footprinting showed that H2A.Z nucleosome core particles were almost structurally indistinguishable from its H2A.1 or native chicken erythrocyte counterparts. While this result is in good agreement with the recently published crystallographic structure of the H2A.Z nucleosome core particle (Suto, R. K., Clarkson, M J., Tremethick, D. J., and Luger, K. (2000) Nat. Struct. Biol. 7, 1121-1124), the ionic strength dependence of the sedimentation coefficient of these particles exhibits a substantial destabilization, which is most likely the result of the histone H2A.Z-H2B dimer binding less tightly to the nucleosome. Analytical ultracentrifuge analysis of the H2A.Z 208-12, a DNA template consisting of 12 tandem repeats of a 208-base pair sequence derived from the sea urchin Lytechinus variegatus 5 S rRNA gene, reconstituted oligonucleosome complexes in the absence of histone H1 shows that their NaCl-dependent folding ability is significantly reduced. These results support the notion that the histone H2A.Z variant may play a chromatin-destabilizing role, which may be important for transcriptional activation.

Ethephon induced oxidative stress in the olive leaf abscission zone enables development of a selective abscission compound.

PubMed

Goldental-Cohen, S; Burstein, C; Biton, I; Ben Sasson, S; Sadeh, A; Many, Y; Doron-Faigenboim, A; Zemach, H; Mugira, Y; Schneider, D; Birger, R; Meir, S; Philosoph-Hadas, S; Irihomovitch, V; Lavee, S; Avidan, B; Ben-Ari, G

2017-05-16

Table olives (Olea europaea L.), despite their widespread production, are still harvested manually. The low efficiency of manual harvesting and the rising costs of labor have reduced the profitability of this crop. A selective abscission treatment, inducing abscission of fruits but not leaves, is crucial for the adoption of mechanical harvesting of table olives. In the present work we studied the anatomical and molecular differences between the three abscission zones (AZs) of olive fruits and leaves. The fruit abscission zone 3 (FAZ3), located between the fruit and the pedicel, was found to be the active AZ in mature fruits and is sensitive to ethephon, whereas FAZ2, between the pedicel and the rachis, is the flower active AZ as well as functioning as the most ethephon induced fruit AZ. We found anatomical differences between the leaf AZ (LAZ) and the two FAZs. Unlike the FAZs, the LAZ is characterized by small cells with less pectin compared to neighboring cells. In an attempt to differentiate between the fruit and leaf AZs, we examined the effect of treating olive-bearing trees with ethephon, an ethylene-releasing compound, with or without antioxidants, on the detachment force (DF) of fruits and leaves 5 days after the treatment. Ethephon treatment enhanced pectinase activity and reduced DF in all the three olive AZs. A transcriptomic analysis of the three olive AZs after ethephon treatment revealed induction of several genes encoding for hormones (ethylene, auxin and ABA), as well as for several cell wall degrading enzymes. However, up-regulation of cellulase genes was found only in the LAZ. Many genes involved in oxidative stress were induced by the ethephon treatment in the LAZ alone. In addition, we found that reactive oxygen species (ROS) mediated abscission in response to ethephon only in leaves. Thus, adding antioxidants such as ascorbic acid or butyric acid to the ethephon inhibited leaf abscission but enhanced fruit abscission. Our findings suggest that

Overexpression of synapsin Ia in the rat calyx of Held accelerates short-term plasticity and decreases synaptic vesicle volume and active zone area

PubMed Central

Vasileva, Mariya; Renden, Robert; Horstmann, Heinz; Gitler, Daniel; Kuner, Thomas

2013-01-01

Synapsins are synaptic vesicle (SV) proteins organizing a component of the reserve pool of vesicles at most central nervous system synapses. Alternative splicing of the three mammalian genes results in multiple isoforms that may differentially contribute to the organization and maintenance of the SV pools. To address this, we first characterized the expression pattern of synapsin isoforms in the rat calyx of Held. At postnatal day 16, synapsins Ia, Ib, IIb and IIIa were present, while IIa—known to sustain repetitive transmission in glutamatergic terminals—was not detectable. To test if the synapsin I isoforms could mediate IIa-like effect, and if this depends on the presence of the E-domain, we overexpressed either synapsin Ia or synapsin Ib in the rat calyx of Held via recombinant adeno-associated virus-mediated gene transfer. Although the size and overall structure of the perturbed calyces remained unchanged, short-term depression and recovery from depression were accelerated upon overexpression of synapsin I isoforms. Using electron microscopic three-dimensional reconstructions we found a redistribution of SV clusters proximal to the active zones (AZ) alongside with a decrease of both AZ area and SV volume. The number of SVs at individual AZs was strongly reduced. Hence, our data indicate that the amount of synapsin Ia expressed in the calyx regulates the rate and extent of short-term synaptic plasticity by affecting vesicle recruitment to the AZ. Finally, our study reveals a novel contribution of synapsin Ia to define the surface area of AZs. PMID:24391547

The pectinate zone is stiff and the arcuate zone determines passive basilar membrane mechanics in the gerbil

NASA Astrophysics Data System (ADS)

Xia, Hongyi; Steele, Charles R.; Puria, Sunil

2018-05-01

The gerbil basilar membrane (BM) differs from other mammalian BMs in that the lower collagen-fiber layer of the pectinate zone (PZ) forms an arch, the upper fiber layer is flat, and ground substance separates the two layers. The role of this arch has been unknown, but can be elucidated by models. In the standard simple beam model (SBM), the upper and lower collagen-fiber layers of the BM are represented as a single layer in both the PZ and the arcuate zone (AZ). In our new arch-beam model (ABM), the upper fiber layer is flat, the lower layer forms an arch in the PZ, and the two layers combine to form the flat portion of the BM in the AZ. This design is incorporated into a 3D finite-element tapered-box model of the cochlea with viscous fluid. We find in the model that the PZ rotates as a rigid body, so its specific properties have little influence, while the AZ thickness and collagen volume fraction primarily determine passive BM mechanics.

14 CFR 93.305 - Flight-free zones and flight corridors.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Flight-free zones and flight corridors. 93... Vicinity of Grand Canyon National Park, AZ § 93.305 Flight-free zones and flight corridors. Except in an... Flight Rules Area within the following flight-free zones: (a) Desert View Flight-free Zone. That airspace...

14 CFR 93.305 - Flight-free zones and flight corridors.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Flight-free zones and flight corridors. 93... Vicinity of Grand Canyon National Park, AZ § 93.305 Flight-free zones and flight corridors. Except in an... Flight Rules Area within the following flight-free zones: (a) Desert View Flight-free Zone. That airspace...

14 CFR 93.305 - Flight-free zones and flight corridors.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Flight-free zones and flight corridors. 93... Vicinity of Grand Canyon National Park, AZ § 93.305 Flight-free zones and flight corridors. Except in an... Flight Rules Area within the following flight-free zones: (a) Desert View Flight-free Zone. That airspace...

14 CFR 93.305 - Flight-free zones and flight corridors.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Flight-free zones and flight corridors. 93... Vicinity of Grand Canyon National Park, AZ § 93.305 Flight-free zones and flight corridors. Except in an... Flight Rules Area within the following flight-free zones: (a) Desert View Flight-free Zone. That airspace...

14 CFR 93.305 - Flight-free zones and flight corridors.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Flight-free zones and flight corridors. 93... Vicinity of Grand Canyon National Park, AZ § 93.305 Flight-free zones and flight corridors. Except in an... Flight Rules Area within the following flight-free zones: (a) Desert View Flight-free Zone. That airspace...

Effect of Pre-Strain on the Fatigue Behavior of Extruded AZ31 Alloys

NASA Astrophysics Data System (ADS)

Wu, Yanjun

2017-09-01

An attempt is made to rationalize the effect of pre-strain history on fatigue behaviors of AZ31 magnesium alloy. Axial fatigue tests were conducted in the extruded and pre-compressioned AZ31 alloy under low cycle total strain control fatigue conditions. The pre-strain process influences the plastic deformation mechanism activated during fatigue deformation, especially during tensile loading, by enhancing the activity of detwinning mechanism. The low-cycle fatigue lifetime of extruded AZ31 alloy can be enhanced by the pre-compression process. And the hysteresis energy was successfully used to predict the low-cycle fatigue lifetime.

The evolution of microstructures, corrosion resistance and mechanical properties of AZ80 joints using ultrasonic vibration assisted welding process

NASA Astrophysics Data System (ADS)

Li, Hui; Zhang, Jiansheng

2017-12-01

The evolution of microstructures, corrosion resistance and mechanical properties of AZ80 joints using an ultrasonic vibration assisted welding process is investigated. The results show that, with ultrasonic vibration treatment, a reliable AZ80 joint without defects is obtained. The coarsening α-Mg grains are refined to about 83.5  ±  3.3 µm and the continuous β-Mg17Al12 phases are broken to granular morphology, owing to the acoustic streaming effect and the cavitation effect evoked by ultrasonic vibration. Both immersion and electrochemical test results indicate that the corrosion resistance of the AZ80 joint welded with ultrasonic vibration is improved, attributed to microstructure evolution. With ultrasonic power of 900 W, the maximum tensile strength of an AZ80 specimen is 261  ±  7.5 MPa and fracture occurs near the heat affected zone of the joint.

System Description for Tank 241-AZ-101 Waste Retrieval Data Acquisition System

DOE Office of Scientific and Technical Information (OSTI.GOV)

ROMERO, S.G.

2000-02-14

The proposed activity provides the description of the Data Acquisition System for Tank 241-AZ-101. This description is documented in HNF-5572, Tank 241-AZ-101 Waste Retrieval Data Acquisition System (DAS). This activity supports the planned mixer pump tests for Tank 241-AZ-101. Tank 241-AZ-101 has been selected for the first full-scale demonstration of a mixer pump system. The tank currently holds over 960,000 gallons of neutralized current acid waste, including approximately 12.7 inches of settling solids (sludge) at the bottom of the tank. As described in Addendum 4 of the FSAR (LMHC 2000a), two 300 HP mixer pumps with associated measurement and monitoringmore » equipment have been installed in Tank 241-AZ-101. The purpose of the Tank 241-AZ-101 retrieval system Data Acquisition System (DAS) is to provide monitoring and data acquisition of key parameters in order to confirm the effectiveness of the mixer pumps utilized for suspending solids in the tank. The suspension of solids in Tank 241-AZ-101 is necessary for pretreatment of the neutralized current acid waste and eventual disposal as glass via the Hanford Waste Vitrification Plant. HNF-5572 provides a basic description of the Tank 241-AZ-101 retrieval system DAS, including the field instrumentation and application software. The DAS is provided to fulfill requirements for data collection and monitoring. This document is not an operations procedure or is it intended to describe the mixing operation. This USQ screening provides evaluation of HNF-5572 (Revision 1) including the changes as documented on ECN 654001. The changes include (1) add information on historical trending and data backup, (2) modify DAS I/O list in Appendix E to reflect actual conditions in the field, and (3) delete IP address in Appendix F per Lockheed Martin Services, Inc. request.« less

Cellular and Pectin Dynamics during Abscission Zone Development and Ripe Fruit Abscission of the Monocot Oil Palm

PubMed Central

Roongsattham, Peerapat; Morcillo, Fabienne; Fooyontphanich, Kim; Jantasuriyarat, Chatchawan; Tragoonrung, Somvong; Amblard, Philippe; Collin, Myriam; Mouille, Gregory; Verdeil, Jean-Luc; Tranbarger, Timothy J.

2016-01-01

The oil palm (Elaeis guineensis Jacq.) fruit primary abscission zone (AZ) is a multi-cell layered boundary region between the pedicel (P) and mesocarp (M) tissues. To examine the cellular processes that occur during the development and function of the AZ cell layers, we employed multiple histological and immunohistochemical methods combined with confocal, electron and Fourier-transform infrared (FT-IR) microspectroscopy approaches. During early fruit development and differentiation of the AZ, the orientation of cell divisions in the AZ was periclinal compared with anticlinal divisions in the P and M. AZ cell wall width increased earlier during development suggesting cell wall assembly occurred more rapidly in the AZ than the adjacent P and M tissues. The developing fruit AZ contain numerous intra-AZ cell layer plasmodesmata (PD), but very few inter-AZ cell layer PD. In the AZ of ripening fruit, PD were less frequent, wider, and mainly intra-AZ cell layer localized. Furthermore, DAPI staining revealed nuclei are located adjacent to PD and are remarkably aligned within AZ layer cells, and remain aligned and intact after cell separation. The polarized accumulation of ribosomes, rough endoplasmic reticulum, mitochondria, and vesicles suggested active secretion at the tip of AZ cells occurred during development which may contribute to the striated cell wall patterns in the AZ cell layers. AZ cells accumulated intracellular pectin during development, which appear to be released and/or degraded during cell separation. The signal for the JIM5 epitope, that recognizes low methylesterified and un-methylesterified homogalacturonan (HG), increased in the AZ layer cell walls prior to separation and dramatically increased on the separated AZ cell surfaces. Finally, FT-IR microspectroscopy analysis indicated a decrease in methylesterified HG occurred in AZ cell walls during separation, which may partially explain an increase in the JIM5 epitope signal. The results obtained

Constitutive turnover of histone H2A.Z at yeast promoters requires the preinitiation complex

PubMed Central

Tramantano, Michael; Sun, Lu; Au, Christy; Labuz, Daniel; Liu, Zhimin; Chou, Mindy; Shen, Chen; Luk, Ed

2016-01-01

The assembly of the preinitiation complex (PIC) occurs upstream of the +1 nucleosome which, in yeast, obstructs the transcription start site and is frequently assembled with the histone variant H2A.Z. To understand the contribution of the transcription machinery in the disassembly of the +1 H2A.Z nucleosome, conditional mutants were used to block PIC assembly. A quantitative ChIP-seq approach, which allows detection of global occupancy change, was employed to measure H2A.Z occupancy. Blocking PIC assembly resulted in promoter-specific H2A.Z accumulation, indicating that the PIC is required to evict H2A.Z. By contrast, H2A.Z eviction was unaffected upon depletion of INO80, a remodeler previously reported to displace nucleosomal H2A.Z. Robust PIC-dependent H2A.Z eviction was observed at active and infrequently transcribed genes, indicating that constitutive H2A.Z turnover is a general phenomenon. Finally, sites with strong H2A.Z turnover precisely mark transcript starts, providing a new metric for identifying cryptic and alternative sites of initiation. DOI: http://dx.doi.org/10.7554/eLife.14243.001 PMID:27438412

In Vitro Studies on the Degradability, Bioactivity, and Cell Differentiation of PRP/AZ31B Mg Alloys Composite Scaffold.

PubMed

Zou, Jian; Shi, Zhongmin; Xu, Hongwei; Li, Xiaolin

2017-01-01

In recent years, more and more methods have been developed to improve the bioactivity of the biodegradable materials in bone tissue regeneration. In present study, we used rat mesenchymal stem cells (rMSCs) to evaluate the outcomes of Mg alloys (AZ31B, Magnesium, and Aluminum) and Platelet-rich plasma (PRP)/Mg alloys on rMSCs biocompatibility and osteogenic differentiation. Water absorption experiments indicated that both bare AZ31B and PRP/AZ31B were capable of absorbing large amounts of water. But the water absorption ratio for PRP/AZ31B was significantly higher than that for bare AZ31B. The degradability experiments implied that both samples degraded at same speed. rMSCs on the surface of AZ31B distributed more and better than those on the AZ31B scaffold. In ALP activity experiment, the activity of rMSCs on the PRP/AZ31B was markedly higher than that on the AZ31B scaffolds on the 7th day and 14th day. qRT-PCR also showed that OPN and OCN were expressed in both samples. OPN and OCN expression in PRP/AZ31B sample were higher than those in bare AZ31B samples. In summary, the in vitro study implied that AZ31B combined with PRP could remarkably improve cell seeding, attachment, proliferation, and differentiation.

Microstructure formation in partially melted zone during gas tungsten arc welding of AZ91 Mg cast alloy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhu Tianping; Chen, Zhan W.; Gao Wei

2008-11-15

During gas tungsten arc (GTA) welding of AZ91 Mg cast alloy, constitutional liquid forms locally in the original interdendritic regions in the partially melted zone (PMZ). The PMZ re-solidification behaviour has not been well understood. In this study, the gradual change of the re-solidification microstructure within PMZ from base metal side to weld metal side was characterised. High cooling rate experiments using Gleeble thermal simulator were also conducted to understand the morphological change of the {alpha}-Mg/{beta}-Mg{sub 17}Al{sub 12} phase interface formed during re-solidification after partial melting. It was found that the original partially divorced eutectic structure has become a moremore » regular eutectic phase in most of the PMZ, although close to the fusion boundary the re-solidified eutectic is again a divorced one. Proceeding the eutectic re-solidification, if the degree of partial melting is sufficiently high, {alpha}-Mg re-solidified with a cellular growth, resulting in a serrated interface between {alpha}-Mg and {alpha}-Mg/{beta}-Mg{sub 17}Al{sub 12} in the weld sample and between {alpha}-Mg and {beta}-Mg{sub 17}Al{sub 12} (fully divorced eutectic) in Gleeble samples. The morphological changes affected by the peak temperature and cooling rate are also explained.« less

The Effect of Process Parameters and Tool Geometry on Thermal Field Development and Weld Formation in Friction Stir Welding of the Alloys AZ31 and AZ61

NASA Astrophysics Data System (ADS)

Zettler, R.; Blanco, A. C.; dos Santos, J. F.; Marya, S.

An increase in the use of magnesium (Mg) in the car manufacturing industry has raised questions concerning its weldability. Friction Stir Welding (FSW) has the advantage of achieving metallic bonding below that of the melting point of the base material thus avoiding many of the metallurgical problems associated with the solidification process. The present study presents the results of a development program carried out to investigate the response of Mg alloys AZ31 and AZ61 to different FSW tool geometries and process parameters. Temperature development across the weld zone was monitored and the produced welds have been subjected to microstructural analysis and mechanical testing. Defect free welds have been produced with optimised FSW-tool and parameters. The micro structure of the welded joint resulted in similar ductility and hardness levels as compared to that of the base material. The results also demonstrated that tool geometry plays a fundamental role in the response of the investigated alloys to the FSW process.

Lysobacter capsici AZ78 produces cyclo(L-Pro-L-Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola.

PubMed

Puopolo, G; Cimmino, A; Palmieri, M C; Giovannini, O; Evidente, A; Pertot, I

2014-10-01

To investigate low molecular weight compounds produced in vitro by Lysobacter capsici AZ78 and their toxic activity against sporangia of plant pathogenic oomycetes. Assays carried out in vitro showed that L. capsici AZ78 drastically inhibits the growth of plant pathogenic oomycetes. Accordingly, the preventive application of culture filtrates of L. capsici AZ78 on grapevine and tomato plants reduced the infections, respectively, caused by Plasmopara (Pl.) viticola and Phytophthora infestans. The subsequent chemical analysis of the culture filtrates of L. capsici AZ78 by spectroscopic (essentially 1D and 2D (1)H NMR and (13)C NMR and ESI MS spectra) and optical methods led to the identification of the 2,5-diketopiperazine cyclo(L-Pro-L-Tyr) that inhibited the development of P. infestans sporangia in vitro and on tomato leaves. Furthermore, a genomic region with high sequence identity with genes coding for a hybrid polyketide synthase and nonribosomal peptide synthetase was detected in L. capsici AZ78. Lysobacter capsici AZ78 produces cyclo(L-Pro-L-Tyr) in vitro that was effective in killing the sporangia of P. infestans and Pl. viticola in vitro. Moreover, this low molecular weight compound prevents the occurrence of late blight lesions when applied on tomato leaves. The application of L. capsici AZ78 cells or its own culture filtrates effectively controls both P. infestans and Pl. viticola. Cyclo(L-Pro-L-Tyr) produced by L. capsici AZ78 is toxic against sporangia of both these oomycetes. These data enforce the potential in the use of Lysobacter members for the control of plant pathogenic oomycetes and provide the basis for the development of new low-impact fungicides based on cyclo(L-Pro-L-Tyr). © 2014 The Society for Applied Microbiology.

Maximal radius of the aftershock zone in earthquake networks

NASA Astrophysics Data System (ADS)

Mezentsev, A. Yu.; Hayakawa, M.

2009-09-01

In this paper, several seismoactive regions were investigated (Japan, Southern California and two tectonically distinct Japanese subregions) and structural seismic constants were estimated for each region. Using the method for seismic clustering detection proposed by Baiesi and Paczuski [M. Baiesi, M. Paczuski, Phys. Rev. E 69 (2004) 066106; M. Baiesi, M. Paczuski, Nonlin. Proc. Geophys. (2005) 1607-7946], we obtained the equation of the aftershock zone (AZ). It was shown that the consideration of a finite velocity of seismic signal leads to the natural appearance of maximal possible radius of the AZ. We obtained the equation of maximal radius of the AZ as a function of the magnitude of the main event and estimated its values for each region.

20. Streetscape, South Coronado Boulevard, looking southwest Righttoleft, AZ198(Margaret B. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. Streetscape, South Coronado Boulevard, looking southwest Right-to-left, AZ-198(Margaret B. Hill House), AZ-199 (Henry H. Hill House), AZ-200 (Henry H. Hill Rental House), AZ-201 (Otto Rosenthal House), AZ-202 (J. M. Stevens House), AZ-203 (L J. Dunagan House), AZ204 (C. W. Beck House), and AZ-205 (F. D. Connor House) face the street - Clifton Townsite, Confluence of Chase Creek & San Francisco River, Clifton, Greenlee County, AZ

Effect of SiC Nanoparticles on Hot Deformation Behavior and Processing Maps of Magnesium Alloy AZ91

PubMed Central

Nie, Kaibo; Kang, Xinkai; Deng, Kunkun; Wang, Ting; Guo, Yachao; Wang, Hongxia

2018-01-01

The hot deformation behavior and processing characteristics of AZ91 alloy and nano-SiCp/AZ91 composite were compared at temperature ranges of 523 K–673 K and strain rates of 0.001–1 s−1. Positive impact of SiC nanoparticles on pinning grain boundaries and inhibiting grain growth was not obvious when deformation temperature was below 623 K, but was remarkable when the temperature was above 623 K. By comparing compressive stress-strain curves of AZ91 alloy and nano-SiCp/AZ91 composites, the addition of nanoparticles could improve the deformation ability of a matrix alloy under high-temperature conditions. There was no essential difference of deformation mechanism between AZ91 alloy and the composite, but hot deformation activation energy of the composite was significantly lower than that of the AZ91 alloy. The AZ91 alloy and the composite had the same workability region of 600 K–673 K and 0.001–1 s−1, while instability region for the composite was reduced compared with that of AZ91 alloy at high temperature. PMID:29389888

Variant Histone H2A.Z Is Globally Localized to the Promoters of Inactive Yeast Genes and Regulates Nucleosome Positioning

PubMed Central

Gévry, Nicolas; Adam, Maryse; Blanchette, Mathieu

2005-01-01

H2A.Z is an evolutionary conserved histone variant involved in transcriptional regulation, antisilencing, silencing, and genome stability. The mechanism(s) by which H2A.Z regulates these various biological functions remains poorly defined, in part due to the lack of knowledge regarding its physical location along chromosomes and the bearing it has in regulating chromatin structure. Here we mapped H2A.Z across the yeast genome at an approximately 300-bp resolution, using chromatin immunoprecipitation combined with tiling microarrays. We have identified 4,862 small regions—typically one or two nucleosomes wide—decorated with H2A.Z. Those “Z loci” are predominantly found within specific nucleosomes in the promoter of inactive genes all across the genome. Furthermore, we have shown that H2A.Z can regulate nucleosome positioning at the GAL1 promoter. Within HZAD domains, the regions where H2A.Z shows an antisilencing function, H2A.Z is localized in a wider pattern, suggesting that the variant histone regulates a silencing and transcriptional activation via different mechanisms. Our data suggest that the incorporation of H2A.Z into specific promoter-bound nucleosomes configures chromatin structure to poise genes for transcriptional activation. The relevance of these findings to higher eukaryotes is discussed. PMID:16248679

Characterization of mussel H2A.Z.2: a new H2A.Z variant preferentially expressed in germinal tissues from Mytilus.

PubMed

Rivera-Casas, Ciro; González-Romero, Rodrigo; Vizoso-Vazquez, Ángel; Cheema, Manjinder S; Cerdán, M Esperanza; Méndez, Josefina; Ausió, Juan; Eirin-Lopez, Jose M

2016-10-01

Histones are the fundamental constituents of the eukaryotic chromatin, facilitating the physical organization of DNA in chromosomes and participating in the regulation of its metabolism. The H2A family displays the largest number of variants among core histones, including the renowned H2A.X, macroH2A, H2A.B (Bbd), and H2A.Z. This latter variant is especially interesting because of its regulatory role and its differentiation into 2 functionally divergent variants (H2A.Z.1 and H2A.Z.2), further specializing the structure and function of vertebrate chromatin. In the present work we describe, for the first time, the presence of a second H2A.Z variant (H2A.Z.2) in the genome of a non-vertebrate animal, the mussel Mytilus. The molecular and evolutionary characterization of mussel H2A.Z.1 and H2A.Z.2 histones is consistent with their functional specialization, supported on sequence divergence at promoter and coding regions as well as on varying gene expression patterns. More precisely, the expression of H2A.Z.2 transcripts in gonadal tissue and its potential upregulation in response to genotoxic stress might be mirroring the specialization of this variant in DNA repair. Overall, the findings presented in this work complement recent reports describing the widespread presence of other histone variants across eukaryotes, supporting an ancestral origin and conserved role for histone variants in chromatin.

Grain-Refined AZ92 Alloy with Superior Strength and Ductility

NASA Astrophysics Data System (ADS)

Lee, Jong Un; Kim, Sang-Hoon; Jo, Wan-Kuen; Hong, Won-Hwa; Kim, Woong; Bae, Jun Ho; Park, Sung Hyuk

2018-03-01

Grain-refined AZ92 (GR-AZ92) alloy with superior tensile properties is developed by adding 1 wt% Zn and a very small amount of SiC (0.17 wt%) to commercial AZ91 alloy for enhancing the solid-solution strengthening effect and refining the crystal grains, respectively. The homogenized GR-AZ92 alloy with an average grain size of 91 μm exhibits a tensile yield strength (TYS) of 125 MPa, ultimate tensile strength (UTS) of 281 MPa, and elongation of 12.1%, which are significantly higher than those of AZ91 alloy with a grain size of 420 μm (TYS of 94 MPa, UTS of 192 MPa, and elongation of 7.0%). The peak-aging time of GR-AZ92 alloy (8 h) is significantly shorter than that of AZ91 alloy (32 h) owing to a larger amount of grain boundaries in the former, which serve as nucleation sites of Mg17Al12 precipitates. A short-aging treatment for less than 1 h of the GR-AZ92 alloy causes an effective improvement in its strength without a significant reduction in its ductility. The 30-min-aged GR-AZ92 alloy has an excellent combination of strength and ductility, with a TYS of 142 MPa, UTS of 304 MPa, and elongation of 8.0%.

Abscission zone development in Setaria viridis and its domesticated relative, Setaria italica.

PubMed

Hodge, John G; Kellogg, Elizabeth A

2016-06-01

Development of an abscission zone (AZ) is needed for dispersal of seeds, and AZ loss was a critical early step in plant domestication. The AZ forms in different tissues in different species of plants, but whether the AZ is developmentally similar wherever it occurs is unknown. AZ development in Setaria viridis was studied as a representative of the previously uncharacterized subfamily Panicoideae. One accession of the wild species S. viridis and two of its domesticate, S. italica, were studied. Strength of the AZ was measured with a force gauge. Anatomy of the AZ was studied throughout development using bright field and confocal microscopy. The force required to remove a spikelet of S. viridis from the parent plant dropped steadily during development, whereas that required to remove spikelets of S. italica increased initially before stabilizing at a high level. Despite the clear difference in tensile strength of the AZ, anatomical differences between S. viridis and S. italica were subtle, and the position of the AZ was not easy to determine in cross sections of pedicel apices. Staining with DAPI showed that nuclei were present up to and presumably through abscission in S. viridis, and acridine orange staining showed much less lignification than in other cereals. The AZ in Setaria is developmentally and anatomically different from that characterized in rice, barley, and many eudicots. In particular, no set of small, densely cytoplasmic cells is obvious. This difference in anatomy could point to differential genetic control of the structure. © 2016 Botanical Society of America.

77 FR 75609 - Approval for Manufacturing Authority; Foreign-Trade Zone 277; Suntech Arizona, Inc. (Solar Panel...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-21

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1873] Approval for Manufacturing Authority; Foreign-Trade Zone 277; Suntech Arizona, Inc. (Solar Panel Manufacturing); Goodyear, AZ Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u...

Corrosion product layers on magnesium alloys AZ31 and AZ61: Surface chemistry and protective ability

NASA Astrophysics Data System (ADS)

Feliu, S.; Llorente, I.

2015-08-01

This paper studies the chemical composition of the corrosion product layers formed on magnesium alloys AZ31 and AZ61 following immersion in 0.6 M NaCl, with a view to better understanding their protective action. Relative differences in the chemical nature of the layers were quantified by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy dispersive analysis of X-ray (EDX) and low-angle X-ray diffraction (XRD). Corrosion behavior was investigated by Electrochemical Impedance Spectroscopy (EIS) and hydrogen evolution measurement. An inhibitive effect from the corrosion product layers was observed from EIS, principally in the case of AZ31, as confirmed by hydrogen evolution tests. A link was found between carbonate enrichment observed by XPS in the surface of the corrosion product layer, concomitant with the increase in the protective properties observed by EIS.

Abnormal macropore formation during double-sided gas tungsten arc welding of magnesium AZ91D alloy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shen Jun; You Guoqiang; Long Siyuan

2008-08-15

One of the major concerns during gas tungsten arc (GTA) welding of cast magnesium alloys is the presence of large macroporosity in weldments, normally thought to occur from the presence of gas in the castings. In this study, a double-sided GTA welding process was adopted to join wrought magnesium AZ91D alloy plates. Micropores were formed in the weld zone of the first side that was welded, due to precipitation of H{sub 2} as the mushy zone freezes. When the reverse side was welded, the heat generated caused the mushy zone in the initial weld to reform. The micropores in themore » initial weld then coalesced and expanded to form macropores by means of gas expansion through small holes that are present at the grain boundaries in the partially melted zone. Macropores in the partially melted zone increase with increased heat input, so that when a filler metal is used the macropores are smaller in number and in size.« less

Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus

PubMed Central

Merelo, Paz; Agustí, Javier; Arbona, Vicent; Costa, Mário L.; Estornell, Leandro H.; Gómez-Cadenas, Aurelio; Coimbra, Silvia; Gómez, María D.; Pérez-Amador, Miguel A.; Domingo, Concha; Talón, Manuel; Tadeo, Francisco R.

2017-01-01

Abscission is a cell separation process by which plants can shed organs such as fruits, leaves, or flowers. The process takes place in specific locations termed abscission zones. In fruit crops like citrus, fruit abscission represents a high percentage of annual yield losses. Thus, understanding the molecular regulation of abscission is of capital relevance to control production. To identify genes preferentially expressed within the citrus fruit abscission zone (AZ-C), we performed a comparative transcriptomics assay at the cell type resolution level between the AZ-C and adjacent fruit rind cells (non-abscising tissue) during ethylene-promoted abscission. Our strategy combined laser microdissection with microarray analysis. Cell wall modification-related gene families displayed prominent representation in the AZ-C. Phylogenetic analyses of such gene families revealed a link between phylogenetic proximity and expression pattern during abscission suggesting highly conserved roles for specific members of these families in abscission. Our transcriptomic data was validated with (and strongly supported by) a parallel approach consisting on anatomical, histochemical and biochemical analyses on the AZ-C during fruit abscission. Our work identifies genes potentially involved in organ abscission and provides relevant data for future biotechnology approaches aimed at controlling such crucial process for citrus yield. PMID:28228766

78 FR 17099 - Safety Zone; BWRC Southwest Showdown 2, Parker, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-20

...The Coast Guard is establishing a temporary safety zone within the Lake Moolvalya region of the navigable waters of the Colorado River in Parker, Arizona for the Blue Water Resort and Casino Southwest Showdown. This temporary safety zone is necessary to provide for the safety of the participants, crew, spectators, participating vessels, and other vessels and users of the waterway. Persons and vessels are prohibited from entering into, transiting through, or anchoring within this safety zone unless authorized by the Captain of the Port or his designated representative.

The Tomato Hybrid Proline-Rich Protein regulates the abcission zone competence to respond to ethylene signals

USDA-ARS?s Scientific Manuscript database

The Tomato Hybrid Proline-Rich Protein (THyPRP) gene was specifically expressed in the tomato (Solanum lycopersicum) flower abscission zone (FAZ), and its stable antisense silencing under the control of an abscission zone (AZ)-specific promoter, Tomato Abscission Polygalacturonase4,significantly inh...

Presynaptic Active Zone Density during Development and Synaptic Plasticity.

PubMed

Clarke, Gwenaëlle L; Chen, Jie; Nishimune, Hiroshi

2012-01-01

Neural circuits transmit information through synapses, and the efficiency of synaptic transmission is closely related to the density of presynaptic active zones, where synaptic vesicles are released. The goal of this review is to highlight recent insights into the molecular mechanisms that control the number of active zones per presynaptic terminal (active zone density) during developmental and stimulus-dependent changes in synaptic efficacy. At the neuromuscular junctions (NMJs), the active zone density is preserved across species, remains constant during development, and is the same between synapses with different activities. However, the NMJ active zones are not always stable, as exemplified by the change in active zone density during acute experimental manipulation or as a result of aging. Therefore, a mechanism must exist to maintain its density. In the central nervous system (CNS), active zones have restricted maximal size, exist in multiple numbers in larger presynaptic terminals, and maintain a constant density during development. These findings suggest that active zone density in the CNS is also controlled. However, in contrast to the NMJ, active zone density in the CNS can also be increased, as observed in hippocampal synapses in response to synaptic plasticity. Although the numbers of known active zone proteins and protein interactions have increased, less is known about the mechanism that controls the number or spacing of active zones. The following molecules are known to control active zone density and will be discussed herein: extracellular matrix laminins and voltage-dependent calcium channels, amyloid precursor proteins, the small GTPase Rab3, an endocytosis mechanism including synaptojanin, cytoskeleton protein spectrins and β-adducin, and a presynaptic web including spectrins. The molecular mechanisms that organize the active zone density are just beginning to be elucidated.

Presynaptic Active Zone Density during Development and Synaptic Plasticity

PubMed Central

Clarke, Gwenaëlle L.; Chen, Jie; Nishimune, Hiroshi

2012-01-01

Neural circuits transmit information through synapses, and the efficiency of synaptic transmission is closely related to the density of presynaptic active zones, where synaptic vesicles are released. The goal of this review is to highlight recent insights into the molecular mechanisms that control the number of active zones per presynaptic terminal (active zone density) during developmental and stimulus-dependent changes in synaptic efficacy. At the neuromuscular junctions (NMJs), the active zone density is preserved across species, remains constant during development, and is the same between synapses with different activities. However, the NMJ active zones are not always stable, as exemplified by the change in active zone density during acute experimental manipulation or as a result of aging. Therefore, a mechanism must exist to maintain its density. In the central nervous system (CNS), active zones have restricted maximal size, exist in multiple numbers in larger presynaptic terminals, and maintain a constant density during development. These findings suggest that active zone density in the CNS is also controlled. However, in contrast to the NMJ, active zone density in the CNS can also be increased, as observed in hippocampal synapses in response to synaptic plasticity. Although the numbers of known active zone proteins and protein interactions have increased, less is known about the mechanism that controls the number or spacing of active zones. The following molecules are known to control active zone density and will be discussed herein: extracellular matrix laminins and voltage-dependent calcium channels, amyloid precursor proteins, the small GTPase Rab3, an endocytosis mechanism including synaptojanin, cytoskeleton protein spectrins and β-adducin, and a presynaptic web including spectrins. The molecular mechanisms that organize the active zone density are just beginning to be elucidated. PMID:22438837

User Manual for the AZ-101 Data Acquisition System (AS-101 DAS)

DOE Office of Scientific and Technical Information (OSTI.GOV)

BRAYTON, D.D.

2000-02-17

User manual for the TK AZ-101 Waste Retrieval System Data Acquisition System. The purpose of this document is to describe use of the AZ-101 Data Acquisition System (AZ-101 DAS). The AZ-101 DAS is provided to fulfill the requirements for data collection and monitoring as defined in Letters of Instruction (LOI) from Numatec Hanford Corporation (NHC) to Fluor Federal Services (FFS). For a complete description of the system, including design, please refer to the AZ-101 DAS System Description document, RPP-5572.

76 FR 78180 - Proposed Modification of Class E Airspace; Douglas, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-16

...-1313; Airspace Docket No. 11-AWP-17] Proposed Modification of Class E Airspace; Douglas, AZ AGENCY... action proposes to modify Class E airspace at Bisbee Douglas International Airport, Douglas, AZ... feet above the surface at Douglas, AZ. Additional controlled airspace is necessary to accommodate...

CESIUM REMOVAL FROM TANKS 241-AN-103 & 241-SX-105 & 241-AZ-101 & 241AZ-102 COMPOSITE FOR TESTING IN BENCH SCALE STEAM REFORMER

DOE Office of Scientific and Technical Information (OSTI.GOV)

DUNCAN JB; HUBER HJ

2011-04-21

This report documents the preparation of three actual Hanford tank waste samples for shipment to the Savannah River National Laboratory (SRNL). Two of the samples were dissolved saltcakes from tank 241-AN-103 (hereafter AN-103) and tank 241-SX-105 (hereafter SX-105); one sample was a supernate composite from tanks 241-AZ-101 and 241-AZ-102 (hereafter AZ-101/102). The preparation of the samples was executed following the test plans LAB-PLAN-10-00006, Test Plan for the Preparation of Samples from Hanford Tanks 241-SX-105, 241-AN-103, 241-AN-107, and LAB-PLN-l0-00014, Test Plan for the Preparation of a Composite Sample from Hanford Tanks 241-AZ-101 and 241-AZ-102 for Steam Reformer Testing at the Savannahmore » River National Laboratory. All procedural steps were recorded in laboratory notebook HNF-N-274 3. Sample breakdown diagrams for AN-103 and SX-105 are presented in Appendix A. The tank samples were prepared in support of a series of treatability studies of the Fluidized Bed Steam Reforming (FBSR) process using a Bench-Scale Reformer (BSR) at SRNL. Tests with simulants have shown that the FBSR mineralized waste form is comparable to low-activity waste glass with respect to environmental durability (WSRC-STI-2008-00268, Mineralization of Radioactive Wastes by Fluidized Bed Steam Reforming (FBSR): Comparisons to Vitreous Waste Forms and Pertinent Durability Testing). However, a rigorous assessment requires long-term performance data from FBSR product formed from actual Hanford tank waste. Washington River Protection Solutions, LLC (WRPS) has initiated a Waste Form Qualification Program (WP-5.2.1-2010-001, Fluidized Bed Steam Reformer Low-level Waste Form Qualification) to gather the data required to demonstrate that an adequate FBSR mineralized waste form can be produced. The documentation of the selection process of the three tank samples has been separately reported in RPP-48824, Sample Selection Process for Bench-Scale Steam Reforming Treatability Studies

Histone H2A.Z is essential for estrogen receptor signaling

PubMed Central

Gévry, Nicolas; Hardy, Sara; Jacques, Pierre-Étienne; Laflamme, Liette; Svotelis, Amy; Robert, François; Gaudreau, Luc

2009-01-01

Incorporation of H2A.Z into the chromatin of inactive promoters has been shown to poise genes for their expression. Here we provide strong evidence that H2A.Z is incorporated into the promoter regions of estrogen receptor (ERα) target genes only upon gene induction, and that, in a cyclic pattern. Moreover, members of the human H2A.Z-depositing complex, p400, also follow the same gene recruitment kinetics as H2A.Z. Importantly, cellular depletion of H2A.Z or p400 leads to a severe defect in estrogen signaling, including loss of estrogen-specific cell proliferation. We find that incorporation of H2A.Z within TFF1 promoter chromatin allows nucleosomes to adopt preferential positions along the DNA translational axis. Finally, we provide evidence that H2A.Z is essential to allow estrogen-responsive enhancer function. Taken together, our results provide strong mechanistic insight into how H2A.Z regulates ERα-mediated gene expression and provide a novel link between H2A.Z–p400 and ERα-dependent gene regulation and enhancer function. PMID:19515975

Fiber Laser Welded AZ31 Magnesium Alloy: The Effect of Welding Speed on Microstructure and Mechanical Properties

NASA Astrophysics Data System (ADS)

Chowdhury, S. H.; Chen, D. L.; Bhole, S. D.; Powidajko, E.; Weckman, D. C.; Zhou, Y.

2012-06-01

This study was aimed at characterizing microstructural change and evaluating tensile and fatigue properties of fiber laser welded AZ31B-H24 Mg alloy with special attention to the effect of welding speed. Laser welding led to the formation of equiaxed dendrites in the fusion zone and columnar dendrites near the fusion zone boundary along with divorced eutectic Mg17Al12 particles and recrystallized grains in the heat-affected zone. The lowest hardness across the weld appeared in the fusion zone. Although the yield strength, ductility, and fatigue life decreased, the hardening capacity increased after laser welding, with a joint efficiency reaching about 90 pct. A higher welding speed resulted in a narrower fusion zone, smaller grain size, higher yield strength, and longer fatigue life, as well as a slightly lower strain-hardening capacity mainly because of the smaller grain sizes. Tensile fracture occurred in the fusion zone, whereas fatigue failure appeared essentially in between the heat-affected zone and the fusion zone. Fatigue cracks initiated from the near-surface welding defects and propagated by the formation of fatigue striations together with secondary cracks.

Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells

PubMed Central

Sundaresan, Srivignesh; Philosoph-Hadas, Sonia; Riov, Joseph; Belausov, Eduard; Kochanek, Betina; Tucker, Mark L.; Meir, Shimon

2015-01-01

In vivo changes in the cytosolic pH of abscission zone (AZ) cells were visualized using confocal microscopic detection of the fluorescent pH-sensitive and intracellularly trapped dye, 2’,7’-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), driven by its acetoxymethyl ester. A specific and gradual increase in the cytosolic pH of AZ cells was observed during natural abscission of flower organs in Arabidopsis thaliana and wild rocket (Diplotaxis tenuifolia), and during flower pedicel abscission induced by flower removal in tomato (Solanum lycopersicum Mill). The alkalization pattern in the first two species paralleled the acceleration or inhibition of flower organ abscission induced by ethylene or its inhibitor 1-methylcyclopropene (1-MCP), respectively. Similarly, 1-MCP pre-treatment of tomato inflorescence explants abolished the pH increase in AZ cells and pedicel abscission induced by flower removal. Examination of the pH changes in the AZ cells of Arabidopsis mutants defective in both ethylene-induced (ctr1, ein2, eto4) and ethylene-independent (ida, nev7, dab5) abscission pathways confirmed these results. The data indicate that the pH changes in the AZ cells are part of both the ethylene-sensitive and -insensitive abscission pathways, and occur concomitantly with the execution of organ abscission. pH can affect enzymatic activities and/or act as a signal for gene expression. Changes in pH during abscission could occur via regulation of transporters in AZ cells, which might affect cytosolic pH. Indeed, four genes associated with pH regulation, vacuolar H+-ATPase, putative high-affinity nitrate transporter, and two GTP-binding proteins, were specifically up-regulated in tomato flower AZ following abscission induction, and 1-MCP reduced or abolished the increased expression. PMID:25504336

Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells.

PubMed

Sundaresan, Srivignesh; Philosoph-Hadas, Sonia; Riov, Joseph; Belausov, Eduard; Kochanek, Betina; Tucker, Mark L; Meir, Shimon

2015-03-01

In vivo changes in the cytosolic pH of abscission zone (AZ) cells were visualized using confocal microscopic detection of the fluorescent pH-sensitive and intracellularly trapped dye, 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), driven by its acetoxymethyl ester. A specific and gradual increase in the cytosolic pH of AZ cells was observed during natural abscission of flower organs in Arabidopsis thaliana and wild rocket (Diplotaxis tenuifolia), and during flower pedicel abscission induced by flower removal in tomato (Solanum lycopersicum Mill). The alkalization pattern in the first two species paralleled the acceleration or inhibition of flower organ abscission induced by ethylene or its inhibitor 1-methylcyclopropene (1-MCP), respectively. Similarly, 1-MCP pre-treatment of tomato inflorescence explants abolished the pH increase in AZ cells and pedicel abscission induced by flower removal. Examination of the pH changes in the AZ cells of Arabidopsis mutants defective in both ethylene-induced (ctr1, ein2, eto4) and ethylene-independent (ida, nev7, dab5) abscission pathways confirmed these results. The data indicate that the pH changes in the AZ cells are part of both the ethylene-sensitive and -insensitive abscission pathways, and occur concomitantly with the execution of organ abscission. pH can affect enzymatic activities and/or act as a signal for gene expression. Changes in pH during abscission could occur via regulation of transporters in AZ cells, which might affect cytosolic pH. Indeed, four genes associated with pH regulation, vacuolar H(+)-ATPase, putative high-affinity nitrate transporter, and two GTP-binding proteins, were specifically up-regulated in tomato flower AZ following abscission induction, and 1-MCP reduced or abolished the increased expression. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Expression profiling of tomato pre-abscission pedicels provides insights into abscission zone properties including competence to respond to abscission signals

PubMed Central

2013-01-01

Background Detachment of plant organs occurs in abscission zones (AZs). During plant growth, the AZ forms, but does not develop further until the cells perceive abscission-promoting signals and initiate detachment. Upon signal perception, abscission initiates immediately; if there is no signal, abscission is not induced and the organ remains attached to the plant. However, little attention has been paid to the genes that maintain competence to respond to the abscission signal in the pre-abscission AZ. Recently, we found that the tomato (Solanum lycopersicum) transcription factors BLIND (Bl), GOBLET (GOB), Lateral suppressor (Ls) and a tomato WUSCHEL homologue (LeWUS) are expressed specifically in pre-abscission tissue, the anthesis pedicel AZs. To advance our understanding of abscission, here we profiled genome-wide gene expression in tomato flower pedicels at the pre-abscission stage. Results We examined the transcriptomes of three tomato flower pedicel regions, the AZ and flanking proximal- (Prox) and distal- (Dis) regions, and identified 89 genes that were preferentially expressed in the AZ compared to both Prox and Dis. These genes included several transcription factors that regulate apical or axillary shoot meristem activity. Also, genes associated with auxin activity were regulated in a Prox-Dis region-specific manner, suggesting that a gradient of auxin exists in the pedicel. A MADS-box gene affecting floral transition was preferentially expressed in the Prox region and other MADS-box genes for floral organ identification were preferentially expressed in Dis, implying that the morphologically similar Prox and Dis regions have distinct identities. We also analyzed the expression of known regulators; in anthesis pedicels, Bl, GOB, Ls and LeWUS were expressed in the vascular cells of the AZ region. However, after an abscission signal, Bl was up-regulated, but GOB, Ls and LeWUS were down-regulated, suggesting that Bl may be a positive regulator of abscission

76 FR 30906 - Foreign-Trade Zone 277-Western Maricopa County, AZ; Application for Reorganization Under...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-27

...'s standard 2,000-acre activation limit for a general-purpose zone project. The application was... Pointe Business Park, Waddell Road and Litchfield Road, Surprise; Site 3 (235 acres)--within the 1,600-acre Palm Valley 303 Industrial Park, Camelback Road and State Road 303, Goodyear; and, Site 4 (320...

Fracture bone healing and biodegradation of AZ31 implant in rats.

PubMed

Iglesias, C; Bodelón, O G; Montoya, R; Clemente, C; Garcia-Alonso, M C; Rubio, J C; Escudero, M L

2015-04-17

The ideal temporary implant should offer enough mechanical support to allow healing of the fracture and then biodegrade and be resorbed by metabolic mechanisms without causing any toxic effect. The aim of this research has been to simultaneously study in situ bone healing and the biodegradation of AZ31 Mg alloy as an osteosynthesis material. The in vivo study was carried out in AZ31 implants with and without Mg-fluoride coating inserted in un-fractured and fractured femurs of Wistar rats for long experimentation time, from 1 to 13 months, by means of computed tomography, histological and histomorphometric analysis. Tomography analysis showed the bone healing and biodegradation of AZ31 implants. The fracture is healed in 100% of the animals, and AZ31 maintains its mechanical integrity throughout the healing process. Biodegradation was monitored, quantifying the evolution of gas over time by 3D composition of tomography images. In all the studied groups, gas pockets disappear with time as a result of the diffusion process through soft tissues. Histomorphometric studies reveal that after 13 months the 46.32% of AZ31 alloy has been resorbed. The resorption of the coated and uncoated AZ31 implants inserted in fractured femurs after 1, 9 and 13 months does not have statistically significant differences. There is a balance between the biodegradation of AZ31 and bone healing which allows the use of AZ31 to be proposed as an osteosynthesis material.

77 FR 73415 - Approval for Manufacturing Authority; Foreign-Trade Zone 277; Sub-Zero, Inc. (Refrigerators and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-10

... Authority; Foreign-Trade Zone 277; Sub-Zero, Inc. (Refrigerators and Freezers); Goodyear, AZ Pursuant to its... Foreign Trade Zone, Inc., grantee of FTZ 277, has requested manufacturing authority on behalf of Sub-Zero... procedures within FTZ 277 on behalf of Sub-Zero, Inc., as described in the application and Federal Register...

H2A.Z Acidic Patch Couples Chromatin Dynamics to Regulation of Gene Expression Programs during ESC Differentiation

PubMed Central

Subramanian, Vidya; Mazumder, Aprotim; Surface, Lauren E.; Butty, Vincent L.; Fields, Paul A.; Alwan, Allison; Torrey, Lillian; Thai, Kevin K.; Levine, Stuart S.; Bathe, Mark; Boyer, Laurie A.

2013-01-01

The histone H2A variant H2A.Z is essential for embryonic development and for proper control of developmental gene expression programs in embryonic stem cells (ESCs). Divergent regions of amino acid sequence of H2A.Z likely determine its functional specialization compared to core histone H2A. For example, H2A.Z contains three divergent residues in the essential C-terminal acidic patch that reside on the surface of the histone octamer as an uninterrupted acidic patch domain; however, we know little about how these residues contribute to chromatin structure and function. Here, we show that the divergent amino acids Gly92, Asp97, and Ser98 in the H2A.Z C-terminal acidic patch (H2A.ZAP3) are critical for lineage commitment during ESC differentiation. H2A.Z is enriched at most H3K4me3 promoters in ESCs including poised, bivalent promoters that harbor both activating and repressive marks, H3K4me3 and H3K27me3 respectively. We found that while H2A.ZAP3 interacted with its deposition complex and displayed a highly similar distribution pattern compared to wild-type H2A.Z, its enrichment levels were reduced at target promoters. Further analysis revealed that H2A.ZAP3 was less tightly associated with chromatin, suggesting that the mutant is more dynamic. Notably, bivalent genes in H2A.ZAP3 ESCs displayed significant changes in expression compared to active genes. Moreover, bivalent genes in H2A.ZAP3 ESCs gained H3.3, a variant associated with higher nucleosome turnover, compared to wild-type H2A.Z. We next performed single cell imaging to measure H2A.Z dynamics. We found that H2A.ZAP3 displayed higher mobility in chromatin compared to wild-type H2A.Z by fluorescent recovery after photobleaching (FRAP). Moreover, ESCs treated with the transcriptional inhibitor flavopiridol resulted in a decrease in the H2A.ZAP3 mobile fraction and an increase in its occupancy at target genes indicating that the mutant can be properly incorporated into chromatin. Collectively, our work suggests

Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nishibuchi, Ikuno; Department of Radiation Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima; Department of Radiation Oncology, Hiroshima Prefectural Hospital, Hiroshima

2014-07-15

Purpose: The reorganization of damaged chromatin plays an important role in the regulation of the DNA damage response. A recent study revealed the presence of 2 vertebrate H2A.Z isoforms, H2A.Z-1 and H2A.Z-2. However, the roles of the vertebrate H2A.Z isoforms are still unclear. Thus, in this study we examined the roles of the vertebrate H2A.Z isoforms in chromatin reorganization after the induction of DNA double-strand breaks (DSBs). Methods and Materials: To examine the dynamics of H2A.Z isoforms at damaged sites, we constructed GM0637 cells stably expressing each of the green fluorescent protein (GFP)-labeled H2A.Z isoforms, and performed fluorescence recovery after photobleaching (FRAP)more » analysis and inverted FRAP analysis in combination with microirradiation. Immunofluorescence staining using an anti-RAD51 antibody was performed to study the kinetics of RAD51 foci formation after 2-Gy irradiation of wild-type (WT), H2A.Z-1- and H2A.Z-2-deficient DT40 cells. Colony-forming assays were also performed to compare the survival rates of WT, H2A.Z-1-, and H2A.Z-2-deficient DT40 cells with control, and H2A.Z-1- and H2A.Z-2-depleted U2OS cells after irradiation. Results: FRAP analysis revealed that H2A.Z-2 was incorporated into damaged chromatin just after the induction of DSBs, whereas H2A.Z-1 remained essentially unchanged. Inverted FRAP analysis showed that H2A.Z-2 was released from damaged chromatin. These findings indicated that H2A.Z-2 was exchanged at DSB sites immediately after the induction of DSBs. RAD51 focus formation after ionizing irradiation was disturbed in H2A.Z-2-deficient DT40 cells but not in H2A.Z-1-deficient cells. The survival rate of H2A.Z-2-deficient cells after irradiation was lower than those of WT and H2A.Z-1- DT40 cells. Similar to DT40 cells, H2A.Z-2-depleted U2OS cells were also radiation-sensitive compared to control and H2A.Z-1-depleted cells. Conclusions: We found that vertebrate H2A.Z-2 is involved in the regulation of

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Photometric Analysis of Eclipsing Binary Az Vir

NASA Astrophysics Data System (ADS)

Neugarten, Andrew; Akiba, Tatsuya; Gokhale, Vayujeet

2018-06-01

We present photometric analysis of the eclipsing binary star system Az Vir. Standard BVR filter data were obtained using the 17-inch PlaneWave Instruments CDK telescope at the Truman State University Observatory in Kirksville, Mo and the 31-inch NURO telescope at the Lowell Observatory complex in Flagstaff, AZ. We apply an eight-term truncated Fourier fit to the light curves generated from these data to confirm the classification of Az Vir as a W Ursae Majoris-type eclipsing variable, using criteria specified by Rucinski (1997). We also calculate the values for the O’Connell Effect Ratio (OER) and the Light Curve Asymmetry (LCA) to quantify the asymmetry in the BVR light curves. In addition, we use data provided by the SuperWASP mission to perform long term O-C (observed minus calculated) analysis on the system to determine if and how its period is changing.

Tank 241-AZ-102 Privatization Push Mode Core Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

RASMUSSEN, J.H.

1999-08-02

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for samples obtained from tank 241-AZ-102. The purpose of this sampling event is to obtain information about the characteristics of the contents of 241-AZ-102 required to satisfy the Data Quality Objectives For TWRS Privatization Phase I: Confirm Tank TIS An Appropriate Feed Source For High-Level Waste Feed Batch X(HLW DQO) (Nguyen 1999a), Data Quality Objectives For TWRS Privatization Phase 1: Confirm Tank TIS An Appropriate Feed Source For Low-Activity Waste Feed Batch X (LAW DQO) (Nguyen 1999b), Low Activity Waste andmore » High Level Waste Feed Data Quality Objectives (L&H DQO) (Patello et al. 1999) and Characterization Data Needs for Development, Design, and Operation of Retrieval Equipment Developed through the Data Quality Objective Process (Equipment DQO) (Bloom 1996). The Tank Characterization Technical Sampling Basis document (Brown et al. 1998) indicates that these issues, except the Equipment DQO apply to tank 241-AZ-102 for this sampling event. The Equipment DQO is applied for shear strength measurements of the solids segments only. Poppiti (1999) requires additional americium-241 analyses of the sludge segments. Brown et al. (1998) also identify safety screening, regulatory issues and provision of samples to the Privatization Contractor(s) as applicable issues for this tank. However, these issues will not be addressed via this sampling event. Reynolds et al. (1999) concluded that information from previous sampling events was sufficient to satisfy the safety screening requirements for tank 241 -AZ-102. Push mode core samples will be obtained from risers 15C and 24A to provide sufficient material for the chemical analyses and tests required to satisfy these data quality objectives. The 222-S Laboratory will extrude core samples, composite the liquids and solids, perform chemical

ANALYSIS RESULTS FOR BUILDING 241 702-AZ A TRAIN

DOE Office of Scientific and Technical Information (OSTI.GOV)

DUNCAN JB; FRYE JM; COOKE CA

2006-12-13

This report presents the analyses results for three samples obtained under RPP-PLAN-28509, Sampling and Analysis Plan for Building 241 702-AZ A Train. The sampling and analysis was done in response to problem evaluation request number PER-2004-6139, 702-AZ Filter Rooms Need Radiological Cleanup Efforts.

Influence of artificial biological fluid composition on the biocorrosion of potential orthopedic Mg-Ca, AZ31, AZ91 alloys.

PubMed

Gu, X N; Zheng, Y F; Chen, L J

2009-12-01

The electrochemical behavior of potential orthopedic Mg-Ca, AZ31 and AZ91 alloys was studied in Hank's solution, Dulbecco's Modified Eagle's Medium (DMEM) and serum-containing medium (DMEM adding 10% fetal bovine serum (DMEM+FBS)) over a 7 day immersion period. The biocorrosion of the above three alloys for various immersion time intervals was investigated by linear polarization and electrochemical impedance spectroscopy (EIS). After 7 day immersion, potentiodynamic polarization tests were carried out and the surface morphologies of experimental samples were examined by scanning electron microscopy (SEM) observation complemented by energy-disperse spectrometer (EDS) analysis. It was shown that the corrosion of magnesium alloys was influenced by the composition of the solution. The results indicated that chloride ion could reduce the corrosion resistance and the hydrocarbonate ions could induce rapid surface passivation. The adsorbed amino acid on the experimental magnesium alloys' surface increased their polarization resistance and reduced current densities. The influence of the serum protein on corrosion was found to be associated with the magnesium alloy compositions. A Mg-Ca alloy exhibited an increased corrosion rate in the presence of serum protein. An AZ31 alloy showed an increased corrosion rate in DMEM+FBS in the initial 3 day immersion and the corrosion rate decreased thereafter. An AZ91 alloy, with high Al content, showed a reduced corrosion rate with the addition of FBS into DMEM.

Global regulation of H2A.Z localization by the INO80 chromatin remodeling enzyme is essential for genome integrity

PubMed Central

Papamichos-Chronakis, Manolis; Watanabe, Shinya; Rando, Oliver J.; Peterson, Craig L.

2010-01-01

Summary INO80 is an evolutionarily conserved, ATP-dependent chromatin remodeling enzyme that plays roles in transcription, DNA repair, and replication. Here, we show that yeast INO80 facilitates these diverse processes at least in part by controlling genome-wide distribution of the histone variant H2A.Z. In the absence of INO80, H2A.Z nucleosomes are mis-localized, and H2A.Z levels at promoters show reduced responsiveness to transcriptional changes, suggesting that INO80 controls H2A.Z dynamics. Additionally, we demonstrate that INO80 has a novel histone exchange activity in which the enzyme can replace nucleosomal H2A.Z/H2B with free H2A/H2B dimers. Genetic interactions between ino80 and htz1 support a model in which INO80 catalyzes the removal of unacetylated H2A.Z from chromatin as a novel mechanism to promote genome stability. PMID:21241891

241-AZ Tank Farm Construction Extent of Condition Review for Tank Integrity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Barnes, Travis J.; Boomer, Kayle D.; Gunter, Jason R.

2013-07-30

This report provides the results of an extent of condition construction history review for tanks 241-AZ-101 and 241-AZ-102. The construction history of the 241-AZ tank farm has been reviewed to identify issues similar to those experienced during tank AY-102 construction. Those issues and others impacting integrity are discussed based on information found in available construction records, using tank AY-102 as the comparison benchmark. In the 241-AZ tank farm, the second DST farm constructed, both refractory quality and tank and liner fabrication were improved.

Histone variant H2A.Z.2 mediates proliferation and drug sensitivity of malignant melanoma

PubMed Central

Vardabasso, Chiara; Gaspar-Maia, Alexandre; Hasson, Dan; Pünzeler, Sebastian; Valle-Garcia, David; Straub, Tobias; Keilhauer, Eva C.; Strub, Thomas; Dong, Joanna; Panda, Taniya; Chung, Chi-Yeh; Yao, Jonathan L.; Singh, Rajendra; Segura, Miguel F.; Fontanals-Cirera, Barbara; Verma, Amit; Mann, Matthias; Hernando, Eva; Hake, Sandra B.; Bernstein, Emily

2015-01-01

SUMMARY Histone variants are emerging as key regulatory molecules in cancer. Here we report a novel role for the H2A.Z isoform H2A.Z.2 as a driver of malignant melanoma. H2A.Z.2 is highly expressed in metastatic melanoma, correlates with decreased patient survival, and is required for cellular proliferation. Our integrated genomic analyses reveal that H2A.Z.2 controls the transcriptional output of E2F target genes in melanoma cells. These genes are highly expressed and display a distinct signature of H2A.Z occupancy. We identify BRD2 as an H2A.Z interacting protein, whose levels are also elevated in melanoma. We further demonstrate that H2A.Z.2 regulated genes are bound by BRD2 and E2F1 in a H2A.Z.2-dependent manner. Importantly, H2A.Z.2 deficiency sensitizes melanoma cells to chemotherapy and targeted therapies. Collectively, our findings implicate H2A.Z.2 as a mediator of cell proliferation and drug sensitivity in malignant melanoma, holding translational potential for novel therapeutic strategies. PMID:26051178

The histone variant H2A.Z promotes efficient cotranscriptional splicing in S. cerevisiae

PubMed Central

Neves, Lauren T.; Douglass, Stephen; Spreafico, Roberto; Venkataramanan, Srivats; Kress, Tracy L.; Johnson, Tracy L.

2017-01-01

In eukaryotes, a dynamic ribonucleic protein machine known as the spliceosome catalyzes the removal of introns from premessenger RNA (pre-mRNA). Recent studies show the processes of RNA synthesis and RNA processing to be spatio–temporally coordinated, indicating that RNA splicing takes place in the context of chromatin. H2A.Z is a highly conserved histone variant of the canonical histone H2A. In Saccharomyces cerevisiae, H2A.Z is deposited into chromatin by the SWR-C complex, is found near the 5′ ends of protein-coding genes, and has been implicated in transcription regulation. Here we show that splicing of intron-containing genes in cells lacking H2A.Z is impaired, particularly under suboptimal splicing conditions. Cells lacking H2A.Z are especially dependent on a functional U2 snRNP (small nuclear RNA [snRNA] plus associated proteins), as H2A.Z shows extensive genetic interactions with U2 snRNP-associated proteins, and RNA sequencing (RNA-seq) reveals that introns with nonconsensus branch points are particularly sensitive to H2A.Z loss. Consistently, H2A.Z promotes efficient spliceosomal rearrangements involving the U2 snRNP, as H2A.Z loss results in persistent U2 snRNP association and decreased recruitment of downstream snRNPs to nascent RNA. H2A.Z impairs transcription elongation, suggesting that spliceosome rearrangements are tied to H2A.Z's role in elongation. Depletion of disassembly factor Prp43 suppresses H2A.Z-mediated splice defects, indicating that, in the absence of H2A.Z, stalled spliceosomes are disassembled, and unspliced RNAs are released. Together, these data demonstrate that H2A.Z is required for efficient pre-mRNA splicing and indicate a role for H2A.Z in coordinating the kinetics of transcription elongation and splicing. PMID:28446598

Flow behaviour of magnesium alloy AZ31B processed by equal-channel angular pressing

NASA Astrophysics Data System (ADS)

Arun, M. S.; Chakkingal, U.

2014-08-01

Magnesium alloys are characterised by their low density, high specific strength and stiffness. But, the potential application of Mg is limited by its low room-temperature ductility & formability. Formability can be improved by developing an ultrafine grained (UFG) structure. Equal channel angular pressing (ECAP) is a well known process that can be used to develop an ultrafine grained microstructure. The aim of this study was to investigate the flow behaviour of AZ31B magnesium alloy after ECAP. The specimen was subjected to three passes of ECAP with a die angle of 120° using processing route Bc. The processing temperature was 523 K for the first pass and 423 K for the subsequent two passes. The microstructure characterisation was done. Compression tests of ECAPed and annealed specimens were carried out at strain rates of 0.01 - 1s-1 and deformation temperatures of 200 - 300°C using computer servo-controlled Gleeble-3800 system. The value of activation energy Q and the empirical materials constants of A and n were determined. The equations relating flow stress and Zener-Hollomon parameter were proposed. In the case annealed AZ31, the activation energy was determined to be 154 kJ/mol, which was slightly higher than the activation energy of 144 kJ/mol for ECAPed AZ31.

Active learning-based information structure analysis of full scientific articles and two applications for biomedical literature review.

PubMed

Guo, Yufan; Silins, Ilona; Stenius, Ulla; Korhonen, Anna

2013-06-01

Techniques that are capable of automatically analyzing the information structure of scientific articles could be highly useful for improving information access to biomedical literature. However, most existing approaches rely on supervised machine learning (ML) and substantial labeled data that are expensive to develop and apply to different sub-fields of biomedicine. Recent research shows that minimal supervision is sufficient for fairly accurate information structure analysis of biomedical abstracts. However, is it realistic for full articles given their high linguistic and informational complexity? We introduce and release a novel corpus of 50 biomedical articles annotated according to the Argumentative Zoning (AZ) scheme, and investigate active learning with one of the most widely used ML models-Support Vector Machines (SVM)-on this corpus. Additionally, we introduce two novel applications that use AZ to support real-life literature review in biomedicine via question answering and summarization. We show that active learning with SVM trained on 500 labeled sentences (6% of the corpus) performs surprisingly well with the accuracy of 82%, just 2% lower than fully supervised learning. In our question answering task, biomedical researchers find relevant information significantly faster from AZ-annotated than unannotated articles. In the summarization task, sentences extracted from particular zones are significantly more similar to gold standard summaries than those extracted from particular sections of full articles. These results demonstrate that active learning of full articles' information structure is indeed realistic and the accuracy is high enough to support real-life literature review in biomedicine. The annotated corpus, our AZ classifier and the two novel applications are available at http://www.cl.cam.ac.uk/yg244/12bioinfo.html

241-AZ Farm Annulus Extent of Condition Baseline Inspection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Engeman, Jason K.; Girardot, Crystal L.; Vazquez, Brandon J.

2013-05-15

This report provides the results of the comprehensive annulus visual inspection for tanks 241- AZ-101 and 241-AZ-102 performed in fiscal year 2013. The inspection established a baseline covering about 95 percent of the annulus floor for comparison with future inspections. Any changes in the condition are also included in this document.

Seismicity and Tectonics of the West Kaibab Fault Zone, AZ

NASA Astrophysics Data System (ADS)

Wilgus, J. T.; Brumbaugh, D. S.

2014-12-01

The West Kaibab Fault Zone (WKFZ) is the westernmost bounding structure of the Kaibab Plateau of northern Arizona. The WKFZ is a branching complex of high angle, normal faults downthrown to the west. There are three main faults within the WKFZ, the Big Springs fault with a maximum of 165 m offset, the Muav fault with 350 m of displacement, and the North Road fault having a maximum throw of approximately 90 m. Mapping of geologically recent surface deposits at or crossing the fault contacts indicates that the faults are likely Quaternary with the most recent offsets occurring <1.6 Ma. Slip rates are estimated to be less than 0.2 mm/yr. No historic fault slip has been documented. The WKFZ is one of the most seismically active areas in Arizona and lies within the Northern Arizona Seismic Belt (NASB), which stretches across northern Arizona trending NW-SE. The data set for this study includes 156 well documented events with the largest being a M5.75 in 1959 and including a swarm of seven earthquakes in 2012. The seismic data set (1934-2014) reveals that seismic activity clusters in two regions within the study area, the Fredonia cluster located in the NW corner of the study area and the Kaibab cluster located in the south central portion of the study area. The fault plane solutions to date indicate NE-SW to EW extension is occurring in the study area. Source relationships between earthquakes and faults within the WKFZ have not previously been studied in detail. The goal of this study is to use the seismic data set, the available data on faults, and the regional physiography to search for source relationships for the seismicity. Analysis includes source parameters of the earthquake data (location, depth, and fault plane solutions), and comparison of this output to the known faults and areal physiographic framework to indicate any active faults of the WKFZ, or suggested active unmapped faults. This research contributes to a better understanding of the present nature of

Effect of Al Addition on Microstructure of AZ91D

NASA Astrophysics Data System (ADS)

Joshi, Utsavi; Babu, Nadendla Hari

Casting is a net shape or near net shape forming process so work-hardening will not be applicable for improving properties of magnesium cast alloys. Grain refinement, solid-solution strengthening, precipitation hardening and specially designed heat treatment are the techniques used to enhance the properties of these alloys. This research focusses on grain refinement of magnesium alloy AZ91D, which is a widely used commercial cast alloy. Recently, Al-B based master alloys have shown potential in grain refining AZ91D. A comparative study of the grain refinement of AZ91D by addition of 0.02wt%B, 0.04wt%B, 0.1wt%B, 0.5wt%B and 1.0wt%B of A1-5B master alloy and equivalent amount of solute element aluminium is described in this paper. Hardness profile of AZ91D alloyed with boron and aluminium is compared.

N terminus of Swr1 binds to histone H2AZ and provides a platform for subunit assembly in the chromatin remodeling complex.

PubMed

Wu, Wei-Hua; Wu, Chwen-Huey; Ladurner, Andreas; Mizuguchi, Gaku; Wei, Debbie; Xiao, Hua; Luk, Ed; Ranjan, Anand; Wu, Carl

2009-03-06

Variant histone H2AZ-containing nucleosomes are involved in the regulation of gene expression. In Saccharomyces cerevisiae, chromatin deposition of histone H2AZ is mediated by the fourteen-subunit SWR1 complex, which catalyzes ATP-dependent exchange of nucleosomal histone H2A for H2AZ. Previous work defined the role of seven SWR1 subunits (Swr1 ATPase, Swc2, Swc3, Arp6, Swc5, Yaf9, and Swc6) in maintaining complex integrity and H2AZ histone replacement activity. Here we examined the function of three additional SWR1 subunits, bromodomain containing Bdf1, actin-related protein Arp4 and Swc7, by analyzing affinity-purified mutant SWR1 complexes. We observed that depletion of Arp4 (arp4-td) substantially impaired the association of Bdf1, Yaf9, and Swc4. In contrast, loss of either Bdf1 or Swc7 had minimal effects on overall complex integrity. Furthermore, the basic H2AZ histone replacement activity of SWR1 in vitro required Arp4, but not Bdf1 or Swc7. Thus, three out of fourteen SWR1 subunits, Bdf1, Swc7, and previously noted Swc3, appear to have roles auxiliary to the basic histone replacement activity. The N-terminal region of the Swr1 ATPase subunit is necessary and sufficient to direct association of Bdf1 and Swc7, as well as Arp4, Act1, Yaf9 and Swc4. This same region contains an additional H2AZ-H2B specific binding site, distinct from the previously identified Swc2 subunit. These findings suggest that one SWR1 enzyme might be capable of binding two H2AZ-H2B dimers, and provide further insight on the hierarchy and interdependency of molecular interactions within the SWR1 complex.

Greening America's Capitals - Phoenix, AZ

EPA Pesticide Factsheets

This report shows design concepts to make pedestrians and bicyclists safer while maintaining on-street parking and providing space for a future streetcar or trolley in Phoenix, AZ. It also shows green infrastructure strategies for arid places.

Biofunctional composite coating architectures based on polycaprolactone and nanohydroxyapatite for controlled corrosion activity and enhanced biocompatibility of magnesium AZ31 alloy.

PubMed

Zomorodian, A; Garcia, M P; Moura E Silva, T; Fernandes, J C S; Fernandes, M H; Montemor, M F

2015-03-01

In this work a biofunctional composite coating architecture for controlled corrosion activity and enhanced cellular adhesion of AZ31 Mg alloys is proposed. The composite coating consists of a polycaprolactone (PCL) matrix modified with nanohydroxyapatite (HA) applied over a nanometric layer of polyetherimide (PEI). The protective properties of the coating were studied by electrochemical impedance spectroscopy (EIS), a non-disturbing technique, and the coating morphology was investigated by field emission scanning electron microscopy (FE-SEM). The results show that the composite coating protects the AZ31 substrate. The barrier properties of the coating can be optimized by changing the PCL concentration. The presence of nanohydroxyapatite particles influences the coating morphology and decreases the corrosion resistance. The biocompatibility was assessed by studying the response of osteoblastic cells on coated samples through resazurin assay, confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). The results show that the polycaprolactone to hydroxyapatite ratio affects the cell behavior and that the presence of hydroxyapatite induces high osteoblastic differentiation. Copyright © 2014 Elsevier B.V. All rights reserved.

Corrosion fatigue behaviors of two biomedical Mg alloys - AZ91D and WE43 - In simulated body fluid.

PubMed

Gu, X N; Zhou, W R; Zheng, Y F; Cheng, Y; Wei, S C; Zhong, S P; Xi, T F; Chen, L J

2010-12-01

Magnesium alloys have been recently developed as biodegradable implant materials, yet there has been no study concerning their corrosion fatigue properties under cyclic loading. In this study the die-cast AZ91D (A for aluminum 9%, Z for zinc 1% and D for a fourth phase) and extruded WE43 (W for yttrium 4%, E for rare earth mischmetal 3%) alloys were chosen to evaluate their fatigue and corrosion fatigue behaviors in simulated body fluid (SBF). The die-cast AZ91D alloy indicated a fatigue limit of 50MPa at 10⁷ cycles in air compared to 20MPa at 10⁶ cycles tested in SBF at 37°C. A fatigue limit of 110MPa at 10⁷ cycles in air was observed for extruded WE43 alloy compared to 40MPa at 10⁷ cycles tested in SBF at 37°C. The fatigue cracks initiated from the micropores when tested in air and from corrosion pits when tested in SBF, respectively. The overload zone of the extruded WE43 alloy exhibited a ductile fracture mode with deep dimples, in comparison to a brittle fracture mode for the die-cast AZ91D. The corrosion rate of the two experimental alloys increased under cyclic loading compared to that in the static immersion test. Copyright © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Phoenix, AZ, USA

NASA Image and Video Library

1973-06-22

SL2-03-200 (22 June 1973) --- The city of Phoenix, AZ (33.5N, 112.0W) can be seen in good detail in this color infrared scene. Situated among truck crop agriculture fields, the color infrared photo depicts the vegetated fields as shades of red making the agriculture stand out in this desert environment. To the east, Lake Theodore Roosevelt and dam can be easily seen. Photo credit: NASA

76 FR 30907 - Proposed Foreign-Trade Zone-Eloy, AZ; Application

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-27

... Toltec Road, Eloy; and, proposed Site 4 (293 acres)--Red Rock Industrial Park, located along Interstate 10 and the Union Pacific Railroad line opposite Sasco Road, Red Rock. The sites are owned by the City... Executive Secretary, Foreign-Trade Zones Board, Room 2111, U.S. Department of Commerce, 1401 Constitution...

Joint Analysis of the Full AzTEC Sub-Millimeter Galaxy Data Set

NASA Astrophysics Data System (ADS)

Wilson, Grant; Ade, P.; Aretxaga, I.; Austermann, J.; Bock, J.; Hughes, D.; Kang, Y.; Kim, S.; Lowenthal, J.; Mauskopf, P.; Perera, T.; Scott, K.; Yun, M.

2006-12-01

Using the new AzTEC millimeter-wave camera on the James Clerk Maxwell Telescope (JCMT) in winter 2005/06, we conducted several surveys of the submm galaxy (SMG) population. The AzTEC 1.1 millimeter surveys include both blank-fields (no significant bias or foreground contamination) and regions of known over-densities, and are both large (100-1000 sq. arcmin.) and sensitive ( 1 mJy rms). The unique power of the AzTEC data set lies not only in the size and depth of the individual fields, but in the combined surveyed area that totals over 1 square degree. Hundreds of new sub-millimeter sources have been detected. A joint analysis of all AzTEC surveys will provide important new constraints on many characteristics of the SMG population, including number counts, clustering, and variance. In particular, the large area of the full AzTEC data set provides the first significant measurement of the brightest and most rare of the SMG population. Herein we present the initial combined results and explore the future potential of a complete joint analysis of the full AzTEC SMG data set.

76 FR 18378 - Amendment of Class E Airspace; Taylor, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-04

...-1189; Airspace Docket No. 10-AWP-19] Amendment of Class E Airspace; Taylor, AZ AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action will amend Class E airspace at Taylor Airport, Taylor, AZ, to accommodate aircraft using the CAMBO One Departure, and the Area Navigation (RNAV...

Molecular Mechanism of Active Zone Organization at Vertebrate Neuromuscular Junctions

PubMed Central

Nishimune, Hiroshi

2013-01-01

Organization of presynaptic active zones is essential for development, plasticity, and pathology of the nervous system. Recent studies indicate a trans-synaptic molecular mechanism that organizes the active zones by connecting the pre- and the postsynaptic specialization. The presynaptic component of this trans-synaptic mechanism is comprised of cytosolic active zone proteins bound to the cytosolic domains of voltage-dependent calcium channels (P/Q-, N-, and L-type) on the presynaptic membrane. The postsynaptic component of this mechanism is the synapse organizer (laminin β2) that is expressed by the postsynaptic cell and accumulates specifically on top of the postsynaptic specialization. The pre- and the postsynaptic components interact directly between the extracellular domains of calcium channels and laminin β2 to anchor the presynaptic protein complex in front of the postsynaptic specialization. Hence, the presynaptic calcium channel functions as a scaffolding protein for active zone organization and as an ion-conducting channel for synaptic transmission. In contrast to the requirement of calcium influx for synaptic transmission, the formation of the active zone does not require the calcium influx through the calcium channels. Importantly, the active zones of adult synapses are not stable structures and require maintenance for their integrity. Furthermore, aging or diseases of the central and peripheral nervous system impair the active zones. This review will focus on the molecular mechanisms that organize the presynaptic active zones and summarize recent findings at the neuromuscular junctions and other synapses. PMID:22135013

Effect of mechanical vibrations on the wear behavior of AZ91 Mg alloy

NASA Astrophysics Data System (ADS)

Chaturvedi, V.; Pandel, U.; Sharma, A.

2018-02-01

AZ91 Mg alloy is the most promising alloy used for structural applications. The vibration induced methods are effective and economic viable in term of mechanical properties. Sliding wear tests were performed on AZ91 Mg alloy using a pin-on- disc configuration. Wear rates were measured at 5 N and 10N at a sliding velocity of 1m/s for varied frequency within the range of 5- 25Hz and a constant amplitude of 2mm. Microstructures of worn surfaces and wear debris were characterized by field emission scanning electron microscopy (FESEM). It is observed that wear resistance of vibrated AZ91 alloy at 15Hz frequency ad 2mm amplitude was superior than cast AZ91 Mg alloy. Finer grain size and equiaxed grain shape both are important parameters for better wear resistance in vibrated AZ91 Mg alloys. FESEM analysis revealed that wear is considerably affected due to frictional heat generated by the relative motion between AZ91 Mg alloy and EN31 steel surface. No single mechanism was responsible for material loss.

Identification and preclinical characterization of AZ-23, a novel, selective, and orally bioavailable inhibitor of the Trk kinase pathway.

PubMed

Thress, Kenneth; Macintyre, Terry; Wang, Haiyun; Whitston, Dave; Liu, Zhong-Ying; Hoffmann, Ethan; Wang, Tao; Brown, Jeffrey L; Webster, Kevin; Omer, Charles; Zage, Peter E; Zeng, Lizhi; Zweidler-McKay, Patrick A

2009-07-01

Tropomyosin-related kinases (TrkA, TrkB, and TrkC) are receptor tyrosine kinases that, along with their ligands, the neurotrophins, are involved in neuronal cell growth, development, and survival. The Trk-neurotrophin pathway may also play a role in tumorigenesis through oncogenic fusions, mutations, and autocrine signaling, prompting the development of novel Trk inhibitors as agents for cancer therapy. This report describes the identification of AZ-23, a novel, potent, and selective Trk kinase inhibitor. In vitro studies with AZ-23 showed improved selectivity over previous compounds and inhibition of Trk kinase activity in cells at low nanomolar concentrations. AZ-23 showed in vivo TrkA kinase inhibition and efficacy in mice following oral administration in a TrkA-driven allograft model and significant tumor growth inhibition in a Trk-expressing xenograft model of neuroblastoma. AZ-23 represents a potent and selective Trk kinase inhibitor from a novel series with the potential for use as a treatment for cancer.

Active zones of mammalian neuromuscular junctions: formation, density, and aging.

PubMed

Nishimune, Hiroshi

2012-12-01

Presynaptic active zones are synaptic vesicle release sites that play essential roles in the function and pathology of mammalian neuromuscular junctions (NMJs). The molecular mechanisms of active zone organization use presynaptic voltage-dependent calcium channels (VDCCs) in NMJs as scaffolding proteins. VDCCs interact extracellularly with the muscle-derived synapse organizer, laminin β2 and interact intracellularly with active zone-specific proteins, such as Bassoon, CAST/Erc2/ELKS2alpha, ELKS, Piccolo, and RIMs. These molecular mechanisms are supported by studies in P/Q- and N-type VDCCs double-knockout mice, and they are consistent with the pathological conditions of Lambert-Eaton myasthenic syndrome and Pierson syndrome, which are caused by autoantibodies against VDCCs or by a laminin β2 mutation. During normal postnatal maturation, NMJs maintain the density of active zones, while NMJs triple their size. However, active zones become impaired during aging. Propitiously, muscle exercise ameliorates the active zone impairment in aged NMJs, which suggests the potential for therapeutic strategies. © 2012 New York Academy of Sciences.

ELKS active zone proteins as multitasking scaffolds for secretion

PubMed Central

Held, Richard G.

2018-01-01

Synaptic vesicle exocytosis relies on the tethering of release ready vesicles close to voltage-gated Ca2+ channels and specific lipids at the future site of fusion. This enables rapid and efficient neurotransmitter secretion during presynaptic depolarization by an action potential. Extensive research has revealed that this tethering is mediated by an active zone, a protein dense structure that is attached to the presynaptic plasma membrane and opposed to postsynaptic receptors. Although roles of individual active zone proteins in exocytosis are in part understood, the molecular mechanisms that hold the protein scaffold at the active zone together and link it to the presynaptic plasma membrane have remained unknown. This is largely due to redundancy within and across scaffolding protein families at the active zone. Recent studies, however, have uncovered that ELKS proteins, also called ERC, Rab6IP2 or CAST, act as active zone scaffolds redundant with RIMs. This redundancy has led to diverse synaptic phenotypes in studies of ELKS knockout mice, perhaps because different synapses rely to a variable extent on scaffolding redundancy. In this review, we first evaluate the need for presynaptic scaffolding, and we then discuss how the diverse synaptic and non-synaptic functional roles of ELKS support the hypothesis that ELKS provides molecular scaffolding for organizing vesicle traffic at the presynaptic active zone and in other cellular compartments. PMID:29491150

A Microstructure Study on an AZ31 Magnesium Alloy Tube after Hot Metal Gas Forming Process

NASA Astrophysics Data System (ADS)

Liu, Yi; Wu, Xin

2007-06-01

An AZ31 magnesium alloy tube has been deformed by the hot metal gas forming (HMGF) technique. Microstructures before and after deformation have been investigated by using Electron Backscattered Diffraction (EBSD) and Electron Microscopy. Due to the inhomogeneous distribution by induction heating, there is a temperature gradient distribution along the tube axis. Accordingly, the deformation mechanism is also different. In the middle area of deformation zone where the temperature is ˜410 °C, almost no twinning has been found, whereas at the edge areas of deformation zone where the temperature is ˜200 °C, a high density of twins has been found. EBSD experiments show a weak (0001) fiber texture along the radial direction of the tube before and after deformation in the high-temperature zone. EBSD experiments on the low temperature deformation region were not successful due to the high stored energy. Schmid factor analysis on the EBSD data shows that, despite the (0001) fiber texture, there are still many grains favoring basal slip along both the axis direction and hoop direction.

Active crustal deformation of the El Salvador Fault Zone (ESFZ) using GPS data: Implications in seismic hazard assessment

NASA Astrophysics Data System (ADS)

Staller, Alejandra; Benito, Belen; Jesús Martínez-Díaz, José; Hernández, Douglas; Hernández-Rey, Román; Alonso-Henar, Jorge

2014-05-01

El Salvador, Central America, is part of the Chortis block in the northwestern boundary of the Caribbean plate. This block is interacting with a diffuse triple junction point with the Cocos and North American plates. Among the structures that cut the Miocene to Pleistocene volcanic deposits stands out the El Salvador Fault Zone (ESFZ): It is oriented in N90º-100ºE direction, and it is composed of several structural segments that deform Quaternary deposits with right-lateral and oblique slip motions. The ESFZ is seismically active and capable of producing earthquakes such as the February 13, 2001 with Mw 6.6 (Martínez-Díaz et al., 2004), that seriously affected the population, leaving many casualties. This structure plays an important role in the tectonics of the Chortis block, since its motion is directly related to the drift of the Caribbean plate to the east and not with the partitioning of the deformation of the Cocos subduction (here not coupled) (Álvarez-Gómez et al., 2008). Together with the volcanic arc of El Salvador, this zone constitutes a weakness area that allows the motion of forearc block toward the NW. The geometry and the degree of activity of the ESFZ are not studied enough. However their knowledge is essential to understand the seismic hazard associated to this important seismogenic structure. For this reason, since 2007 a GPS dense network was established along the ESFZ (ZFESNet) in order to obtain GPS velocity measurements which are later used to explain the nature of strain accumulation on major faults along the ESFZ. The current work aims at understanding active crustal deformation of the ESFZ through kinematic model. The results provide significant information to be included in a new estimation of seismic hazard taking into account the major structures in ESFZ.

The Daptocephalus Assemblage Zone (Lopingian), South Africa: A proposed biostratigraphy based on a new compilation of stratigraphic ranges

NASA Astrophysics Data System (ADS)

Viglietti, Pia A.; Smith, Roger M. H.; Angielczyk, Kenneth D.; Kammerer, Christian F.; Fröbisch, Jörg; Rubidge, Bruce S.

2016-01-01

The Dicynodon Assemblage Zone (DiAZ) of South Africa's Karoo Basin is one of the eight biostratigraphic zones of the Beaufort Group. It spans the uppermost Permian strata (Balfour, Teekloof, and Normandien formations) and traditionally has been considered to terminate with the disappearance of Dicynodon lacerticeps at the Permo-Triassic Boundary. We demonstrate that the three index fossils currently used to define the Dicynodon Assemblage Zone (Dicynodon lacerticeps, Theriognathus microps, and Procynosuchus delaharpeae) have first appearance datums (FADs) below its traditionally recognized lower boundary and have ranges mostly restricted to the lower portion of the biozone, well below the Permo-Triassic Boundary. We propose re-establishing Daptocephalus leoniceps as an index fossil for this stratigraphic interval, and reinstating the name Daptocephalus Assemblage Zone (DaAZ) for this unit. Furthermore, the FAD of Lystrosaurus maccaigi in the uppermost reaches of the biozone calls for the establishment of a two-fold subdivision of the current Dicynodon Assemblage Zone. The biostratigraphic utility of Da. leoniceps and other South African dicynodontoids outside of the Karoo Basin is limited due to basinal endemism at the species level and varying temporal ranges of dicynodontoids globally. Therefore, we recommend their use only for correlation within the Karoo Basin at this time. Revision of the stratigraphic ranges of all late Permian tetrapods does not reveal a significant change in faunal diversity between the lower and upper DaAZ. However, the last appearance datums of the abundant taxa Di. lacerticeps, T. microps, P. delaharpeae, and Diictodon feliceps occur below the three extinction phases associated with the end-Permian mass extinction event. Due to northward attenuation of the strata, however, the stratigraphic position of the extinction phases may need to be reconsidered.

Validation of Airborne Visible-Infrared Imaging Spectrometer Data at Ray Mine, AZ

NASA Technical Reports Server (NTRS)

Lang, H.; Baloga, S.

1999-01-01

We validate 1997 Airborne Visible-Infrared Imaging Spectrometer (AVIRIS) reflectance spectra covering 0.4 meu - 2.4 meu from a stable, flat mineralogically characterized man-made target at Ray Mine, AZ, the site for an EPA/NASA assessment of the utility of remote sensing for monitoring acid drainage from an active open pit mine.

The Status of Implementation of Good Manufacturing Practices (GMP) Shredded Fish Production in UMKM Az-Zahrah, Makassar

NASA Astrophysics Data System (ADS)

Latif, Rindam; Dirpan, Andi; Indriani, Sylvia

2017-12-01

Good manufacturing practices (GMP) describes the requirements that an industry should follow throughout the production process starting from sorting raw materials to handling final products. The purpose of this research is to disclose each phase of GMP in making shredded fish and to assess the status of the practices implemented by the UMKM Az-zahrah in producing its product. This descriptive study is conducted by doing interview with owner followed by observation and documentation of all activities related to the production process in order to assess the status of GMP applied by the UMKM Az-zahrah. In assessing the status, we referred to Permenperin RI No.75 / M-Ind / Per / 7/2010 and KBPOM regulation no. HK 03.1.23.04.12.22007 Year 2012. The findings indicate some deviation occurred when UMKM Az-zahrah implemented it, those are (1) there is a pet in production room (2) production employees are not yet wearing work uniform (3) production room has directly access to toilet (4) there is no health label about health and nutritional claims. The status of applied GMP in the UMKM Az-zahrah is at D level (less good) with rating IV.

Presynaptic active zones of mammalian neuromuscular junctions: Nanoarchitecture and selective impairments in aging.

PubMed

Badawi, Yomna; Nishimune, Hiroshi

2018-02-01

Neurotransmitter release occurs at active zones, which are specialized regions of the presynaptic membrane. A dense collection of proteins at the active zone provides a platform for molecular interactions that promote recruitment, docking, and priming of synaptic vesicles. At mammalian neuromuscular junctions (NMJs), muscle-derived laminin β2 interacts with presynaptic voltage-gated calcium channels to organize active zones. The molecular architecture of presynaptic active zones has been revealed using super-resolution microscopy techniques that combine nanoscale resolution and multiple molecular identification. Interestingly, the active zones of adult NMJs are not stable structures and thus become impaired during aging due to the selective degeneration of specific active zone proteins. This review will discuss recent progress in the understanding of active zone nanoarchitecture and the mechanisms underlying active zone organization in mammalian NMJs. Furthermore, we will summarize the age-related degeneration of active zones at NMJs, and the role of exercise in maintaining active zones. Copyright © 2017 Elsevier Ireland Ltd and Japan Neuroscience Society. All rights reserved.

Copper Electrodeposition on a Magnesium Alloy (AZ80) with a U-Shaped Surface

PubMed Central

Huang, Ching An; Yeh, Yu Hu; Lin, Che Kuan; Hsieh, Chen Yun

2014-01-01

Cu electrodeposition was performed on a cylindrical AZ80 substrate with a U-shaped surface. A uniform deposition of Cu was achieved on an AZ80 electrode via galvanostatic etching, followed by Cu electrodeposition in an eco-friendly alkaline Cu plating bath. Improper wetting and lower rotational speeds of the AZ80 electrode resulted in an uneven Cu deposition at the inner upper site of the U-shaped surface during the Cu electroplating process. This wetting effect could be deduced from the variation in the anodic potential during the galvanostatic etching. The corrosion resistance of the Cu-deposited AZ80 electrode can be considerably improved after Ni electroplating. PMID:28788252

First 'Rauisuchian' archosaur (Pseudosuchia, Loricata) for the Middle Triassic Santacruzodon assemblage zone (Santa Maria Supersequence), Rio Grande do Sul State, Brazil.

PubMed

Lacerda, Marcel B; Schultz, Cesar L; Bertoni-Machado, Cristina

2015-01-01

The 'Rauisuchia' are a group of Triassic pseudosuchian archosaurs that displayed a near worldwide distribution. In Brazil, their fossils are found only in the Santa Maria Formation (Paraná Basin) of the Rio Grande do Sul State, specifically in the Middle Triassic Dinodontosaurus assemblage zone (AZ) and the Late Triassic Hyperodapedon AZ (Rauisuchus tiradentes). Between these two cenozones is the Santacruzodon AZ (Middle Triassic), whose record was, until now, restricted to non-mammalian cynodonts and the proterochampsian Chanaresuchus bonapartei. Here we present the first occurrence of a rauisuchian archosaur for this cenozone, from the Schoenstatt outcrop, located near the city of Santa Cruz do Sul and propose a new species, based on biostratigraphical evidence and a comparative osteological analysis.

Effect of Annealing on Microstructure and Tensile Properties of 5052/AZ31/5052 Clad Sheets

NASA Astrophysics Data System (ADS)

Nie, Huihui; Liang, Wei; Chi, Chengzhong; Li, Xianrong; Fan, Haiwei; Yang, Fuqian

2016-05-01

Three-layered 5052Al/AZ31Mg/5052Al (5052/AZ31/5052) clad sheets were fabricated by four-pass rolling and annealed under different conditions. Under the optimal annealing condition, homogeneous and equiaxial grains with an average AZ31 grain size of 5.24 µm were obtained and the maximum values of ultimate tensile strength and elongation of the clad sheet reached 230 MPa and 18%, respectively. Electron backscatter diffraction analysis showed that the AZ31 layer had a typical rolling texture with its c-axis parallel to the normal direction. The fraction of low-angle grain boundaries in the 5052 layer was nearly four times more than that in the AZ31 layer because of different deformation extent and recrystallization driving forces. The textures of Al3Mg2 and Mg17Al12 were similar to that of 5052 because of the deformation coordination during the rolling and recrystallization process. The orientation relationship between Mg17Al12 and AZ31 seemed to be (110) Mg17Al12//(10-11) AZ31.

78 FR 78298 - Proposed Establishment of Class E Airspace; Phoenix, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-26

...-0956; Airspace Docket No. 13-AWP-17] Proposed Establishment of Class E Airspace; Phoenix, AZ AGENCY... rulemaking (NPRM). SUMMARY: This action proposes to establish Class E airspace at the Phoenix VHF Omni-Directional Radio Range Tactical Air Navigation Aid (VORTAC), Phoenix, AZ, to facilitate vectoring of...

76 FR 30299 - Proposed Establishment of Class E Airspace; Kayenta, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-25

...-0393; Airspace Docket No. 11-AWP-2] Proposed Establishment of Class E Airspace; Kayenta, AZ AGENCY... action proposes to establish Class E Airspace at Kayenta Airport, Kayenta, AZ. Controlled airspace is...) standard instrument approach procedures at Kayenta Airport. The FAA is proposing this action to enhance the...

Quantitative regulation of histone variant H2A.Z during cell cycle by ubiquitin proteasome system and SUMO-targeted ubiquitin ligases.

PubMed

Takahashi, Daisuke; Orihara, Yuki; Kitagawa, Saho; Kusakabe, Masayuki; Shintani, Takahiro; Oma, Yukako; Harata, Masahiko

2017-08-01

Quantitative control of histones and histone variants during cell cycle is relevant to their epigenetic functions. We found that the level of yeast histone variant H2A.Z in the G2/M-phase is actively kept low by the ubiquitin proteasome system and SUMO-targeted ubiquitin ligases. Overexpression of H2A.Z induced defects in mitotic progression, suggesting functional importance of this quantitative control.

System Description for Tank 241-AZ-101 Waste Retrieval Data Acquisition System

DOE Office of Scientific and Technical Information (OSTI.GOV)

ROMERO, S.G.

2000-01-10

Describes the hardware and software for the AZ-101 Mixer Pump Data Acquisition System. The purpose of the tank 241-AZ-101 retrieval system Data Acquisition System (DAS) is to provide monitoring and data acquisition of key parameters in order to confirm the effectiveness of the mixer pumps utilized for suspending solids in the tank. The suspension of solids in Tank 241-AZ-101 is necessary for pretreatment of the neutralized current acid waste (NCAW), and eventual disposal as glass via the Hanford Waste Vitrification Plant.

76 FR 2000 - Modification of Class E Airspace; Show Low, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-12

...-0903; Airspace Docket No. 10-AWP-16] Modification of Class E Airspace; Show Low, AZ AGENCY: Federal... Show Low, AZ, to accommodate aircraft using a new Area Navigation (RNAV) Global Positioning System (GPS) Standard Instrument Approach Procedures at Show Low Regional Airport. This will improve the safety and...

Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum)

PubMed Central

Tsuchiya, Mutsumi; Satoh, Shinobu; Iwai, Hiroaki

2015-01-01

After fruit development is triggered by pollination, the abscission zone (AZ) in the fruit pedicel strengthens its adhesion to keep the fruit attached. We previously reported that xyloglucan and arabinan accumulation in the AZ accompanies the shedding of unpollinated flowers. After the fruit has developed and is fully ripened, shedding occurs easily in the AZ due to lignin accumulation. Regulation of cell wall metabolism may play an important role in these processes, but it is not well understood. In the present report, we used immunohistochemistry to visualize changes in the distributions of xyloglucan and arabinan metabolism-related enzymes in the AZs of pollinated and unpollinated flowers, and in ripened fruits. During floral abscission, we observed a gradual increase in polyclonal antibody labeling of expansin in the AZ. The intensities of LM6 and LM15 labeling of arabinan and xyloglucan, respectively, also increased. However, during floral abscission, we observed a large 1 day post anthesis (DPA) peak in the polyclonal antibody labeling of XTH in the AZ, which then decreased. These results suggest that expansin and XTH play important, but different roles in the floral abscission process. During fruit abscission, unlike during floral abscission, no AZ-specific expansin and XTH were observed. Although lignification was seen in the AZ of over-ripe fruit pedicels, secondary cell wall-specific cellulose synthase signals were not observed. This suggests that cellulose metabolism-related enzymes do not play important roles in the AZ prior to fruit abscission. PMID:26029225

Differential deposition of H2A.Z in rice seedling tissue during the day-night cycle.

PubMed

Zhang, Kang; Xu, Wenying; Wang, Chunchao; Yi, Xin; Su, Zhen

2017-03-04

Chromatin structure has an important role in modulating gene expression. The incorporation of histone variants into the nucleosome leads to important changes in the chromatin structure. The histone variant H2A.Z is highly conserved between different species of fungi, animals, and plants. However, dynamic changes to H2A.Z in rice have not been reported during the day-night cycle. In this study, we generated genome wide maps of H2A.Z for day and night time in harvested seedling tissues by combining chromatin immunoprecipitation and high-throughput sequencing. The analysis results for the H2A.Z data sets detected 7099 genes with higher depositions of H2A.Z in seedling tissues harvested at night compared with seedling tissues harvested during the day, whereas 4597 genes had higher H2A.Z depositions in seedlings harvested during the day. The gene expression profiles data suggested that H2A.Z probably negatively regulated gene expression during the day-night cycle and was involved in many important biologic processes. In general, our results indicated that H2A.Z may play an important role in plant responses to the diurnal oscillation process.

A study on the relationships between corrosion properties and chemistry of thermally oxidised surface films formed on polished commercial magnesium alloys AZ31 and AZ61

NASA Astrophysics Data System (ADS)

Feliu, Sebastián; Samaniego, Alejandro; Barranco, Violeta; El-Hadad, A. A.; Llorente, Irene; Serra, Carmen; Galván, J. C.

2014-03-01

This paper studies the changes in chemical composition of the thin oxide surface films induced by heating in air at 200 °C for time intervals from 5 min to 60 min on the freshly polished commercial AZ31 and AZ61 alloys with a view to better understanding their protective properties. This thermal treatment resulted in the formation of layers enriched in metallic aluminium at the interface between the outer MgO surface films and the bulk material. A strong link was found between the degree of metallic Al enrichment in the subsurface layer (from 10 to 15 at.%) observed by XPS (X-ray photoelectron spectroscopy) in the AZ61 treated samples and the increase in protective properties observed by EIS (electrochemical impedance spectroscopy) in the immersion test in 0.6 M NaCl. Heating for 5-60 min in air at 200 °C seems to be an effective, easy to perform and inexpensive method for increasing the corrosion resistance of the AZ61 alloy by approximately two or three times.

76 FR 3570 - Proposed Amendment of Class E Airspace; Taylor, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-20

...-1189; Airspace Docket No. 10-AWP-19] Proposed Amendment of Class E Airspace; Taylor, AZ AGENCY: Federal... proposes to modify Class E airspace at Taylor Airport, Taylor, AZ. Controlled airspace is necessary to accommodate aircraft using the CAMBO One Departure Area Navigation (RNAV) out of Taylor Airport. The FAA is...

78 FR 48303 - Establishment of Class E Airspace; Tuba City, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-08

...-2013-0147; Airspace Docket No. 13-AWP-1] Establishment of Class E Airspace; Tuba City, AZ AGENCY... airspace at the Tuba City VHF Omni-Directional Radio Range Tactical Air Navigational Aid (VORTAC), Tuba City, AZ. In that rule, an error was made in the legal description for Tuba City, identifying the...

Microwave-assisted magnesium phosphate coating on the AZ31 magnesium alloy.

PubMed

Ren, Yufu; Babaie, Elham; Lin, Boren; Bhaduri, Sarit B

2017-08-18

Due to the combination of many unique properties, magnesium alloys have been widely recognized as suitable metallic materials for fabricating degradable biomedical implants. However, the extremely high degradation kinetics of magnesium alloys in the physiological environment have hindered their clinical applications. This paper reports for the first time the use of a novel microwave-assisted coating process to deposit magnesium phosphate (MgP) coatings on the Mg alloy AZ31 and improve its in vitro corrosion resistance. Newberyite and trimagnesium phosphate hydrate (TMP) layers with distinct features were fabricated at various processing times and temperatures. Subsequently, the corrosion resistance, degradation behavior, bioactivity and cytocompatibility of the MgP coated AZ31 samples were investigated. The potentiodynamic polarization tests reveal that the corrosion current density of the AZ31 magnesium alloy in simulated body fluid (SBF) is significantly suppressed by the deposited MgP coatings. Additionally, it is seen that MgP coatings remarkably reduced the mass loss of the AZ31 alloy after immersion in SBF for two weeks and promoted precipitation of apatite particles. The high viability of preosteoblast cells cultured with extracts of coated samples indicates that the MgP coatings can improve the cytocompatibility of the AZ31 alloy. These attractive results suggest that MgP coatings, serving as the protective and bioactive layer, can enhance the corrosion resistance and biological response of magnesium alloys.

Texture Development in a Friction Stir Lap-Welded AZ31B Magnesium Alloy

NASA Astrophysics Data System (ADS)

Naik, B. S.; Chen, D. L.; Cao, X.; Wanjara, P.

2014-09-01

The present study was aimed at characterizing the microstructure, texture, hardness, and tensile properties of an AZ31B-H24 Mg alloy that was friction stir lap welded (FSLWed) at varying tool rotational rates and welding speeds. Friction stir lap welding (FSLW) resulted in the presence of recrystallized grains and an associated hardness drop in the stir zone (SZ). Microstructural investigation showed that both the AZ31B-H24 Mg base metal (BM) and SZ contained β-Mg17Al12 and Al8Mn5 second phase particles. The AZ31B-H24 BM contained a type of basal texture (0001)<110> with the (0001) plane nearly parallel to the rolled sheet surface and <110> directions aligned in the rolling direction. FSLW resulted in the formation of another type of basal texture (0001)<100> in the SZ, where the basal planes (0001) became slightly tilted toward the transverse direction, and the prismatic planes (100) and pyramidal planes (101) exhibited a 30 deg + ( n - 1) × 60 deg rotation ( n = 1, 2, 3, …) with respect to the rolled sheet normal direction, due to the shear plastic flow near the pin surface that occurred from the intense local stirring. With increasing tool rotational rate and decreasing welding speed, the maximum intensity of the basal poles (0001) in the SZ decreased due to a higher degree of dynamic recrystallization that led to a weaker or more random texture. The tool rotational rate and welding speed had a strong effect on the failure load of FSLWed joints. A combination of relatively high welding speed (20 mm/s) and low tool rotational rate (1000 rpm) was observed to be capable of achieving a high failure load. This was attributed to the relatively small recrystallized grains and high intensity of the basal poles in the SZ arising from the low heat input as well as the presence of a small hooking defect.

Removal of hydrogen sulfide by sulfate-resistant Acidithiobacillus thiooxidans AZ11.

PubMed

Lee, Eun Young; Lee, Nae Yoon; Cho, Kyung-Suk; Ryu, Hee Wook

2006-04-01

Toxic H2S gas is an important industrial pollutant that is applied to biofiltration. Here, we examined the effects of factors such as inlet concentration and space velocity on the removal efficiency of a bacterial strain capable of tolerating high sulfate concentrations and low pH conditions. We examined three strains of Acidithiobacillus thiooxidans known to have sulfur-oxidizing activity, and identified strain AZ11 as having the highest tolerance for sulfate. A. thiooxidans AZ11 could grow at pH 0.2 in the presence of 74 g l(-1) sulfate, the final oxidation product of elemental sulfur, in the culture broth. Under these conditions, the specific sulfur oxidation rate was 2.9 g-S g-DCW (dry cell weight)(-1) d(-1). The maximum specific sulfur oxidation rate of A. thiooxidans AZ11 was 21.2 g-S g-DCW(-1) d(-1), which was observed in the presence of 4.2 g-SO4(2-) l(-1) and pH 1.5, in the culture medium. To test the effects of various factors on biofiltration by this strain, A. thiooxidans AZ11 was inoculated into a porous ceramic biofilter. First, a maximum inlet loading of 670 g-S m(-3) h(-1) was applied with a constant space velocity (SV) of 200 h(-1) (residence time, 18 s) and the inlet concentration of H2S was experimentally increased from 200 ppmv to 2200 ppmv. Under these conditions, less than 0.1 ppmv H2S was detected at the biofilter outlet. When the inlet H2S was maintained at a constant concentration of 200 ppmv and the SV was increased from 200 h(-1) to 400 h(-1) (residence time, 9 s), an H2S removal of 99.9% was obtained. However, H2S removal efficiencies decreased to 98% and 94% when the SV was set to 500 h(-1) (residence time, 7.2 s) and 600 h(-1) (residence time, 6 s), respectively. The critical elimination capacity guaranteeing 96% removal of the inlet H2S was determined to be 160 g-S m(-3) h(-1) at a space velocity of 600 h(-1). Collectively, these findings show for the first time that a sulfur oxidizing bacterium has a high sulfate tolerance and a high

First 'Rauisuchian' archosaur (Pseudosuchia, Loricata) for the Middle Triassic Santacruzodon Assemblage Zone (Santa Maria Supersequence), Rio Grande do Sul State, Brazil

PubMed Central

Lacerda, Marcel B.; Schultz, Cesar L.; Bertoni-Machado, Cristina

2015-01-01

The ‘Rauisuchia’ are a group of Triassic pseudosuchian archosaurs that displayed a near worldwide distribution. In Brazil, their fossils are found only in the Santa Maria Formation (Paraná Basin) of the Rio Grande do Sul State, specifically in the Middle Triassic Dinodontosaurus assemblage zone (AZ) and the Late Triassic Hyperodapedon AZ (Rauisuchus tiradentes). Between these two cenozones is the Santacruzodon AZ (Middle Triassic), whose record was, until now, restricted to non-mammalian cynodonts and the proterochampsian Chanaresuchus bonapartei. Here we present the first occurrence of a rauisuchian archosaur for this cenozone, from the Schoenstatt outcrop, located near the city of Santa Cruz do Sul and propose a new species, based on biostratigraphical evidence and a comparative osteological analysis. PMID:25714091

78 FR 44011 - Safety Zone; Bullhead City Regatta; Bullhead City, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-23

... Guard Sector San Diego this year and past years inquiring more about the exclusion of the waterway for... excessive trash resulting from the event. The safety zone is being established this year as in past years to... holidays. FOR FURTHER INFORMATION CONTACT: If you have questions on this rule, call or email Lieutenant...

Planar techniques for fabricating X-ray diffraction gratings and zone plates

NASA Technical Reports Server (NTRS)

Smith, H. I.; Anderson, E. H.; Hawryluk, A. M.; Schattenburg, M. L.

1984-01-01

The state of current planar techniques in the fabrication of Fresnel zone plates and diffraction gratings is reviewed. Among the fabrication techniques described are multilayer resist techniques; scanning electron beam lithography; and holographic lithography. Consideration is also given to: X-ray lithography; ion beam lithography; and electroplating. SEM photographs of the undercut profiles obtained in a type AZ 135OB photoresistor by holographic lithography are provided.

AZ-101 Mixer Pump Test Qualification Test Procedures (QTP)

DOE Office of Scientific and Technical Information (OSTI.GOV)

THOMAS, W.K.

2000-01-10

Describes the Qualification test procedure for the AZ-101 Mixer Pump Data Acquisition System (DAS). The purpose of this Qualification Test Procedure (QTP) is to confirm that the AZ-101 Mixer Pump System has been properly programmed and hardware configured correctly. This QTP will test the software setpoints for the alarms and also check the wiring configuration from the SIMcart to the HMI. An Acceptance Test Procedure (ATP), similar to this QTP will be performed to test field devices and connections from the field.

Effect of Carbon Nanotube on High-Temperature Formability of AZ31 Magnesium Alloy

NASA Astrophysics Data System (ADS)

Hassan, S. Fida; Paramsothy, M.; Gasem, Z. M.; Patel, F.; Gupta, M.

2014-08-01

Room-temperature tensile properties of AZ31 alloy have significantly been improved when reinforced with carbon nanotube via ingot metallurgy process. However, high-temperature (up to 250 °C) elongation-to-failure tensile test of the developed nanocomposite revealed a considerable softening in the AZ31 alloy matrix accompanied by an incredible ductility increment (up to 132%). Microstructural characterization of the fractured samples revealed that the dynamic recrystallization process has induced a complete recrystallization in the AZ31 alloy at a lower temperature (150 °C) followed by substantial grain growth at a higher temperature used in this study. Fractography on the fractured surfaces revealed that the room-temperature mixed brittle-ductile modes of fracture behavior of AZ31 alloy have transformed into a complete ductile mode of fracture at high temperature.

Changing palaeoenvironments and tetrapod populations in the Daptocephalus Assemblage Zone (Karoo Basin, South Africa) indicate early onset of the Permo-Triassic mass extinction

NASA Astrophysics Data System (ADS)

Viglietti, Pia A.; Smith, Roger M. H.; Rubidge, Bruce S.

2018-02-01

Important palaeoenvironmental differences are identified during deposition of the latest Permian Daptocephalus Assemblage Zone (DaAZ) of the South African Beaufort Group (Karoo Supergoup), which is also divided into a Lower and Upper subzone. A lacustrine floodplain facies association showing evidence for higher water tables and subaqueous conditions on the floodplains is present in Lower DaAZ. The change to well-drained floodplain facies association in the Upper DaAZ is coincident with a faunal turnover as evidenced by the last appearance of the dicynodont Dicynodon lacerticeps, the therocephalian Theriognathus microps, the cynodont Procynosuchus delaharpeae, and first appearance of the dicynodont Lystrosaurus maccaigi within the Ripplemead member. Considering the well documented 3-phased extinction of Karoo tetrapods during the Permo-Triassic Mass Extinction (PTME), the facies transition between the Lower and Upper DaAZ represents earlier than previously documented palaeoenvironmental changes associated with the onset of this major global biotic crisis.

75 FR 65255 - Proposed Modification of Class E Airspace; Show Low, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-22

...-0903; Airspace Docket No. 10-AWP-16] Proposed Modification of Class E Airspace; Show Low, AZ AGENCY... action proposes to modify Class E airspace at Show Low, AZ to accommodate new Area Navigation (RNAV) Global Positioning System (GPS) Standard Instrument Approach Procedures (SIAPs) at Show Low Regional...

76 FR 64041 - Proposed Amendment of Class E Airspace; Show Low, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-17

...-1023; Airspace Docket No. 11-AWP-15] Proposed Amendment of Class E Airspace; Show Low, AZ AGENCY... action proposes to modify Class E airspace at Show Low Regional Airport, Show Low, AZ. Controlled...) standard instrument approach procedures at Show Low Regional Airport. The FAA is proposing this action to...

Microarray analysis of the abscission-related transcriptome in tomato flower abscission zone in response to auxin depletion

USDA-ARS?s Scientific Manuscript database

Abscission, the separation of organs from the parent plant, results in postharvest quality loss in many fresh produce. The process is initiated by changes in the auxin gradient across the abscission zone (AZ), is triggered by ethylene and may be accelerated by postharvest stresses. Although changes ...

33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Activities Far East Marine... SECURITY GENERAL COAST GUARD AREAS, DISTRICTS, SECTORS, MARINE INSPECTION ZONES, AND CAPTAIN OF THE PORT ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone. (a...

33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Activities Far East Marine... SECURITY GENERAL COAST GUARD AREAS, DISTRICTS, SECTORS, MARINE INSPECTION ZONES, AND CAPTAIN OF THE PORT ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone. (a...

PRODUCTION AND TRANSPORT OF CARBON DIOXIDE IN A CONTAMINATED VADOSE ZONE: A STABLE AND RADIOACTIVE CARBON ISOTOPE STUDY

EPA Science Inventory

Analyses of soil gas compositions and stable and radioactive carbon isotopes in the vadose zone above an alluvial aquifer were conducted at an organic solvent disposal site in southeast Phoenix, AZ. The study investigated the source and movement of carbon dioxide above a plume of...

Multiblock grid generation with automatic zoning

NASA Technical Reports Server (NTRS)

Eiseman, Peter R.

1995-01-01

An overview will be given for multiblock grid generation with automatic zoning. We shall explore the many advantages and benefits of this exciting technology and will also see how to apply it to a number of interesting cases. The technology is available in the form of a commercial code, GridPro(registered trademark)/az3000. This code takes surface geometry definitions and patterns of points as its primary input and produces high quality grids as its output. Before we embark upon our exploration, we shall first give a brief background of the environment in which this technology fits.

Project W-314 specific test and evaluation plan for AZ tank farm upgrades

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hays, W.H.

1998-08-12

The purpose of this Specific Test and Evaluation Plan (STEP) is to provide a detailed written plan for the systematic testing of modifications made by the addition of the SN-631 transfer line from the AZ-O1A pit to the AZ-02A pit by the W-314 Project. The STEP develops the outline for test procedures that verify the system`s performance to the established Project design criteria. The STEP is a lower tier document based on the W-314 Test and Evaluation P1 an (TEP). Testing includes Validations and Verifications (e.g., Commercial Grade Item Dedication activities, etc), Factory Tests and Inspections (FTIs), installation tests andmore » inspections, Construction Tests and Inspections (CTIs), Acceptance Test Procedures (ATPs), Pre-Operational Test Procedures (POTPs), and Operational Test Procedures (OTPs). The STEP will be utilized in conjunction with the TEP for verification and validation.« less

Unc-51 controls active zone density and protein composition by downregulating ERK signaling.

PubMed

Wairkar, Yogesh P; Toda, Hirofumi; Mochizuki, Hiroaki; Furukubo-Tokunaga, Katsuo; Tomoda, Toshifumi; Diantonio, Aaron

2009-01-14

Efficient synaptic transmission requires the apposition of neurotransmitter release sites opposite clusters of postsynaptic neurotransmitter receptors. Transmitter is released at active zones, which are composed of a large complex of proteins necessary for synaptic development and function. Many active zone proteins have been identified, but little is known of the mechanisms that ensure that each active zone receives the proper complement of proteins. Here we use a genetic analysis in Drosophila to demonstrate that the serine threonine kinase Unc-51 acts in the presynaptic motoneuron to regulate the localization of the active zone protein Bruchpilot opposite to glutamate receptors at each synapse. In the absence of Unc-51, many glutamate receptor clusters are unapposed to Bruchpilot, and ultrastructural analysis demonstrates that fewer active zones contain dense body T-bars. In addition to the presence of these aberrant synapses, there is also a decrease in the density of all synapses. This decrease in synaptic density and abnormal active zone composition is associated with impaired evoked transmitter release. Mechanistically, Unc-51 inhibits the activity of the MAP kinase ERK to promote synaptic development. In the unc-51 mutant, increased ERK activity leads to the decrease in synaptic density and the absence of Bruchpilot from many synapses. Hence, activated ERK negatively regulates synapse formation, resulting in either the absence of active zones or the formation of active zones without their proper complement of proteins. The Unc-51-dependent inhibition of ERK activity provides a potential mechanism for synapse-specific control of active zone protein composition and release probability.

RadNet Air Data From Yuma, AZ

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Yuma, AZ from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

RadNet Air Data From Phoenix, AZ

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Phoenix, AZ from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

RadNet Air Data From Tucson, AZ

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Tucson, AZ from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

A new aspect of flower abscission: involvement of a specific alkalization of the cytosol in the abscission zone cells

USDA-ARS?s Scientific Manuscript database

The correlation between organ abscission and pH changes in the abscission zone (AZ) cells, visualized by the pH-sensitive and intracellularly trapped dye, 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein-acetoxymethyl (BCECF-AM) ester derivative, combined with confocal microscopy was studied. ...

Session Overview and AzTEC Instrument Performance

NASA Astrophysics Data System (ADS)

Wilson, Grant; Ade, P. A.; Aretxaga, I.; Austermann, J.; Bock, J. J.; Hughes, D.; Kang, Y.; Kim, S.; Lowenthal, J.; Mauskopf, P.; Scott, K.; Yun, M.

2006-12-01

AzTEC is a new 144 element bolometer receiver destined as a first-generation instrument for the Large Millimeter Telescope. >From November 2005 and through January 2006, AzTEC made science observations at the 15m James Clerk Maxwell Telescope (JCMT). Approximately 1/2 of the available time was spent mapping the submillimeter galaxy population in blank and biased fields. Overall, over 1 square degree of sky was mapped with uniform coverage in each of five primary fields making this the largest set of surveys of the submillimeter galaxy population ever performed. Hundreds of new submillimeter galaxies have been detected. Here we discuss the instrument, our mapping technique, and a brief summary of the data reduction process. We conclude with a brief summary of the overall impact of these surveys on our understanding of the submillimeter galaxy population.

Active crustal deformation of the El Salvador Fault Zone by integrating geodetic, seismological and geological data: application in seismic hazard assessment

NASA Astrophysics Data System (ADS)

Staller, A.; Benito, B.; Martínez-Díaz, J.; Hernández, D.; Hernández-Rey, R.

2013-05-01

El Salvador, Central America, is part of the Chortis block in the northwestern boundary of the Caribbean plate. This block is interacting with a diffuse triple junction point with the Cocos and North American plates. Among the structures that cut the Miocene to Pleistocene volcanic deposits stands out the El Salvador Fault Zone (ESFZ): It is oriented in N90-100E direction, and it is composed of several structural segments that deform Quaternary deposits with right-lateral and oblique slip motions. The ESFZ is seismically active and capable of producing earthquakes such as the February 13, 2001 with Mw 6.6 (Martínez-Díaz et al., 2004), that seriously affected the population, leaving many casualties. This structure plays an important role in the tectonics of the Chortis block, since its motion is directly related to the drift of the Caribbean plate to the east and not with the partitioning of the deformation of the Cocos subduction (here not coupled) (Álvarez-Gómez et al., 2008). Together with the volcanic arc of El Salvador, this zone constitutes a weakness area that allows the motion of forearc block toward the NW. The geometry and the degree of activity of the ESFZ are not studied enough. However their knowledge is essential to understand the seismic hazard associated to this important seismogenic structure. For this reason, since 2007 a GPS dense network was established along the ESFZ (ZFESNet) in order to obtain GPS velocity measurements which are later used to explain the nature of strain accumulation on major faults along the ESFZ. The current work aims at understanding active crustal deformation of the ESFZ through kinematic model. The results provide significant information to be included in a new estimation of seismic hazard taking into account the major structures in ESFZ.

78 FR 57923 - Arizona Disaster #AZ-00029

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-20

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13766 and 13767] Arizona Disaster AZ-00029 AGENCY: Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative... completed loan applications to: U.S. Small Business Administration, Processing and Disbursement Center...

CONCENTRATIONS OF PESTICIDE FROM DERMAL SURFACES: A COMPARISON OF NHEXAS & AZ BORDER SAMPLES

EPA Science Inventory

NHEXAS-AZ was a statewide survey designed to gather data on the distributions of exposure from various media. Results of intensive sampling were obtained from 179 homes. Border-AZ was a similar study focusing on homes within 40 km of the Arizona-Mexico Border; similar results...

Effect of Ca and RE additions on microstructures and tensile properties of AZ31 alloys

NASA Astrophysics Data System (ADS)

Fu, Li; Le, Qichi; Tang, Yan; Sun, Jingying; Jia, Yonghui; Song, Zetian

2018-05-01

Microstructures and tensile properties of AZ31 magnesium alloys with the same amount of Ca and RE (Gd + La) additions are investigated. The results show that Al2Ca new phases form after adding Ca elements, Al2Gd and Al11La3 new phases form after adding Gd and La elements, and formations of Al-Ca and Al-RE phases could decrease Mg17Al12 phases and refine grains. Al2Ca and Al11La3 phases are crushed into granules because of severe deformation during hot extrusion, while Al2Gd phases are not. Room temperature (TR) and 150 °C (T150°C) tensile tests results reveal that both AZ31-1.5Ca and AZ31-1.5RE as-extruded alloys exhibit superior comprehensive tensile properties when compared to AZ31 as-extruded alloy, however, AZ31-1.5Ca as-extruded alloy could be a better choice in view of the costs. Textures images of as-extruded alloys indicate that 1.5 wt% Ca and RE additions affects little on textures of AZ31 as-extruded alloy, therefore, morphologies of second phases and average grain sizes are the leading cause of tensile properties of as-extruded alloys.

Star and Dust Formation Activities in AzTEC-3, a Starburst Galaxy at z = 5.3

NASA Astrophysics Data System (ADS)

Dwek, Eli; Staguhn, Johannes G.; Arendt, Richard G.; Capak, Peter L.; Kovacs, Attila; Benford, Dominic J.; Fixsen, Dale; Karim, Alexander; Leclercq, Samuel; Maher, Stephen F.; Moseley, Samuel H.; Schinnerer, Eva; Sharp, Elmer H.

2011-09-01

Analyses of high-redshift ultraluminous infrared (IR) galaxies traditionally use the observed optical to submillimeter spectral energy distribution (SED) and estimates of the dynamical mass as observational constraints to derive the star formation rate (SFR), the stellar mass, and age of these objects. An important observational constraint neglected in the analysis is the mass of dust giving rise to the IR emission. In this paper we add this constraint to the analysis of AzTEC-3. Adopting an upper limit to the mass of stars and a bolometric luminosity for this object, we construct different stellar and chemical evolutionary scenarios, constrained to produce the inferred dust mass and observed luminosity before the associated stellar mass exceeds the observational limit. We use the PÉGASE population synthesis code and a chemical evolution model to follow the evolution of the galaxy's SED and its stellar and dust masses as a function of galactic age for seven different stellar initial mass functions (IMFs). We find that the model with a Top Heavy IMF provided the most plausible scenario consistent with the observational constraints. In this scenario the dust formed over a period of ~200 Myr, with an SFR of ~500 M sun yr-1. These values for the age and SFR in AzTEC-3 are significantly higher and lower, respectively, from those derived without the dust mass constraint. However, this scenario is not unique, and others cannot be completely ruled out because of the prevailing uncertainties in the age of the galaxy, its bolometric luminosity, and its stellar and dust masses. A robust result of our models is that all scenarios require most of the radiating dust mass to have been accreted in molecular clouds. Our new procedure highlights the importance of a multiwavelength approach, and of the use of dust evolution models in constraining the age and the star formation activity and history in galaxies.

STAR AND DUST FORMATION ACTIVITIES IN AzTEC-3, A STARBURST GALAXY AT z = 5.3

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dwek, Eli; Staguhn, Johannes G.; Arendt, Richard G.

2011-09-01

Analyses of high-redshift ultraluminous infrared (IR) galaxies traditionally use the observed optical to submillimeter spectral energy distribution (SED) and estimates of the dynamical mass as observational constraints to derive the star formation rate (SFR), the stellar mass, and age of these objects. An important observational constraint neglected in the analysis is the mass of dust giving rise to the IR emission. In this paper we add this constraint to the analysis of AzTEC-3. Adopting an upper limit to the mass of stars and a bolometric luminosity for this object, we construct different stellar and chemical evolutionary scenarios, constrained to producemore » the inferred dust mass and observed luminosity before the associated stellar mass exceeds the observational limit. We use the PEGASE population synthesis code and a chemical evolution model to follow the evolution of the galaxy's SED and its stellar and dust masses as a function of galactic age for seven different stellar initial mass functions (IMFs). We find that the model with a Top Heavy IMF provided the most plausible scenario consistent with the observational constraints. In this scenario the dust formed over a period of {approx}200 Myr, with an SFR of {approx}500 M{sub sun} yr{sup -1}. These values for the age and SFR in AzTEC-3 are significantly higher and lower, respectively, from those derived without the dust mass constraint. However, this scenario is not unique, and others cannot be completely ruled out because of the prevailing uncertainties in the age of the galaxy, its bolometric luminosity, and its stellar and dust masses. A robust result of our models is that all scenarios require most of the radiating dust mass to have been accreted in molecular clouds. Our new procedure highlights the importance of a multiwavelength approach, and of the use of dust evolution models in constraining the age and the star formation activity and history in galaxies.« less

Effect of microstructure on the zinc phosphate conversion coatings on magnesium alloy AZ91

NASA Astrophysics Data System (ADS)

Van Phuong, Nguyen; Moon, Sungmo; Chang, Doyon; Lee, Kyu Hwan

2013-01-01

The effect of the microstructure, particularly of β-Mg17Al12 phase, on the formation and growth of zinc phosphate conversion coatings on magnesium alloy AZ91 (AZ91) was studied. The zinc phosphate coatings were formed on AZ91 with different microstructures produced by heat treatment. The effect of the microstructure on the zinc phosphate coatings were examined using optical microscope (OM), X-ray diffraction (XRD), coatings weight and etching weight balances, scanning electron microscopy (SEM) and salt immersion test. Results showed that as-cast AZ91 contained a high volume fraction of the β-Mg17Al12 phase and it was dissolved into α-Mg phase during heat treatment at 400 °C. The β-phase became center for hydrogen evolution during phosphating reaction (cathodic sites). The decreased volume fraction of the β-phase caused decreasing both coatings weight and etching weight of the phosphating process. However, it increased the crystal size of the coatings and improved corrosion resistance of AZ91 by immersing in 0.5 M NaCl solution. Results also showed that the structure of the zinc phosphate conversion on AZ91 consisted of two layers: an outer crystal Zn3(PO4)2·4H2O (hopeite) and an inner which was mainly composed of MgZn2(PO4)2 and Mg3(PO4)2. A mechanism for the formation of two layers of the coatings was also proposed in this study.

Comparing the performance plateau in adult cochlear implant patients using HINT and AzBio.

PubMed

Massa, Sean T; Ruckenstein, Michael J

2014-04-01

This study aims to characterize the performance plateau in adult cochlear implant recipients after the initial postimplantation increase by using word recognition testing and an explicit definition of performance plateau. Retrospective review. Urban, tertiary referral center. One hundred twenty-five patients with 138 devices tested with AzBio were matched to 130 patients with 138 devices tested with HINT based on performed on CNC monosyllable tests. Patient's performance was measured overtime using AzBio and HINT tests to determine when and at what score their performance reached a plateau. Time from implantation to reach a performance plateau and plateau score with each test. Thirty-four devices reached a HINT plateau and 30 devices reached an AzBio plateau. Patients reached plateaus at similar times postoperatively using HINT and AzBio, 18.8 and 16.5 weeks, respectively (p = 0.476). Five patients tested with HINT plateaued at scores of 99% to 100%, whereas no patients plateaued above 92% with AzBio. Patients reached a plateau in performance at similar median times using AzBio and HINT, despite the ceiling effect of HINT in some patients. Most patients who reach a plateau did so within 4 months, but exactly when and if a patient's performance plateaus varies significantly among individuals. Further study is required to determine which test best reflects when a patient reaches his or her maximal performance in natural listening conditions.

Effects of Neodymium and Calcium on the Thermal Stability of AZ71 Magnesium Alloys

NASA Astrophysics Data System (ADS)

Yue, Cheng-Feng; Huang, Shi-Jei; Chen, Jhewn-Kuang; Li, Hsien-Tsung; Chan, Kam-Shau

2018-03-01

The effects of an addition of 0-2 wt% Nd on thermal stability of 0-3 wt% Ca-containing modified AZ71 magnesium alloys was investigated. The ignition temperature was found to increase from that of AZ71, 574, to 825 °C with the addition of 0.5 wt% Ca and 1 wt% Nd. The ignition temperature was further increased to 1114 °C when 3 wt% Ca was added. The Ca- and Nd-added AZ71 was isothermally maintained at a temperature of 500 °C in air for 12 h. The MgO-CaO-Nd2O3 formed on the surface to improve the thermal stability of the AZ71-xCa-yNd alloys. While both the tensile strength and ductility decreased with the Ca concentration in the alloy, an addition of 1 wt% Nd was found able to alleviate the degradation effects of Ca on the tensile strength and ductility at 170 °C. Both solid solution formation and precipitation strengthening contributed to the increase in toughness. AZ71 containing 0.5-2 wt% Ca and 1 wt% Nd provides the optimum combination of ignition resistance and mechanical properties.

Biochemistry Games: "AZ-Quiz" and "Jeopardy!"

ERIC Educational Resources Information Center

Rostejnska, Milada; Klimova, Helena

2011-01-01

"AZ-Quiz" and "Jeopardy!" are popular television shows and serve as the basis for in-class games designed to support and diversify chemistry instruction at the high school level. Both games were created in Microsoft PowerPoint, which is an easily accessible and controllable instrument that enables the creation of engaging animation. The use of…

Formation of intermetallic compound coating on magnesium AZ91 cast alloy

NASA Astrophysics Data System (ADS)

Zhu, Tianping; Gao, Wei

2009-08-01

This study describes an intermetallic compound coating formed on AZ91 Mg cast alloy. The Al sputtered on AZ91 cast alloy reacted with substrate during a short period of heat treatment at 435°C, resulting in the formation of a continuous intermetallic compound layer. The short period treatment has the advantage of minimizing the negative effect on the microstructure of substrate and the mechanical properties, comparing with the reported diffusion coatings. DSC measurement and examination on the cross-section of Al sputtered samples show that local melting occurred along the Al/substrate interface at the temperature range between 430~435°C. The formation mechanism of intermetallic compound coating is proposed in terms of the local melting at Al/substrate interface. The salt water immersion test showed significant improvement in corrosion resistance of the intermetallic compound coated AZ91 cast alloy compared with the as-cast alloys.

Corrosion and mechanical performance of AZ91 exposed to simulated inflammatory conditions.

PubMed

Brooks, Emily K; Der, Stephanie; Ehrensberger, Mark T

2016-03-01

Magnesium (Mg) and its alloys, including Mg-9%Al-1%Zn (AZ91), are biodegradable metals with potential use as temporary orthopedic implants. Invasive orthopedic procedures can provoke an inflammatory response that produces hydrogen peroxide (H2O2) and an acidic environment near the implant. This study assessed the influence of inflammation on both the corrosion and mechanical properties of AZ91. The AZ91 samples in the inflammatory protocol were immersed for three days in a complex biologically relevant electrolyte (AMEM culture media) that contained serum proteins (FBS), 150 mM of H2O2, and was titrated to a pH of 5. The control protocol immersed AZ91 samples in the same biologically relevant electrolyte (AMEM & FBS) but without H2O2 and the acid titration. After 3 days all samples were switched into fresh AMEM & FBS for an additional 3-day immersion. During the initial immersion, inflammatory protocol samples showed increased corrosion rate determined by mass loss testing, increased Mg and Al ion released to solution, and a completely corroded surface morphology as compared to the control protocol. Although corrosion in both protocols slowed once the test electrolyte solution was replaced at 3 days, the samples originally exposed to the simulated inflammatory conditions continued to display enhanced corrosion rates as compared to the control protocol. These lingering effects may indicate the initial inflammatory corrosion processes modified components of the surface oxide and corrosion film or initiated aggressive localized processes that subsequently left the interface more vulnerable to continued enhanced corrosion. The electrochemical properties of the interfaces were also evaluated by EIS, which found that the corrosion characteristics of the AZ91 samples were potentially influenced by the role of intermediate adsorption layer processes. The increased corrosion observed for the inflammatory protocol did not affect the flexural mechanical properties of the AZ91

Arsenic Removal from Drinking Water by Adsorptive Media USEPA Demonstration Project at Rimrock AZ Final Performance Evaluation Report

EPA Science Inventory

This report documents the activities performed during and the results obtained from the arsenic removal treatment technology demonstration project at the Arizona Water Company (AWC) facility in Rimrock, AZ. The objectives of the project were to evaluate: 1) the effectiveness of ...

Grain Refinement of AZ31 Magnesium Alloy Weldments by AC Pulsing Technique

NASA Astrophysics Data System (ADS)

Kishore Babu, N.; Cross, C. E.

2012-11-01

The current study has investigated the influence of alternating current pulsing on the structure and mechanical properties of AZ31 magnesium alloy gas tungsten arc (GTA) weldments. Autogenous full penetration bead-on-plate GTA welds were made under a variety of conditions including variable polarity (VP), variable polarity mixed (VPM), alternating current (AC), and alternating current pulsing (ACPC). AC pulsing resulted in significant refinement of weld metal when compared with the unpulsed conditions. AC pulsing leads to relatively finer and more equiaxed grain structure in GTA welds. In contrast, VP, VPM, and AC welding resulted in predominantly columnar grain structures. The reason for this grain refinement may be attributed to the periodic variations in temperature gradient and solidification rate associated with pulsing as well as weld pool oscillation observed in the ACPC welds. The observed grain refinement was shown to result in an appreciable increase in fusion zone hardness, tensile strength, and ductility.

Arizonans on Edge...So Why Not Involved? AZ Views. Volume 2, Issue 1

ERIC Educational Resources Information Center

Morrison Institute for Public Policy, Arizona State University, 2009

2009-01-01

What a difference a year makes. In June 2008, "AZ Views" reported that "Arizonans have a strong sense of job security, despite the national economic slump and the state's budget crisis." That is no longer true, as this edition of "AZ Views" shows, and Arizona's economic situation arguably is the best example of the…

Microstructure and Properties of 5083 Al/1060 Al/AZ31 Composite Plate Fabricated by Explosive Welding

NASA Astrophysics Data System (ADS)

Yang, Suyuan; Bao, Jiawei

2018-03-01

A 5083 Al/1060 Al/AZ31 composite plate was fabricated by explosive welding. The microstructure and properties of the composite plate were investigated after explosive welding. The results showed that all bonding interfaces were wavy interfaces. With an increasing distance from the detonation point, the wavelength and the amplitude also increased. The EDS results indicated that a 5-μm diffusion layer was observed at the 1060 Al/AZ31 layer, including the Mg2Al3 phase. Adiabatic shear bands and twin structures were observed in AZ31. The shear bond strength of the 5083 Al/1060 Al interface was 60 MPa, and the shear bond strength of the 1060 Al/AZ31 interface was 84 MPa.

ARSENIC REMOVAL FROM DRINKING WATER BY ADSORPTIVE MEDIA, USEPA DEMONSTRATION PROJECT AT RIMROCK, AZ, SIX-MONTH EVALUATION REPORT

EPA Science Inventory

This report documents the activities performed during and the results obtained from the first six months of the arsenic removal treatment technology demonstration project at Rimrock, AZ. The objectives of the project are to evaluate the effectiveness of AdEdge Arsenic Package Uni...

Tank 241-AZ-101 Mixer Pump Test Vapor Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

TEMPLETON, A.M.

2000-03-06

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for vapor samples obtained during the operation of mixer pumps in tank 241-AZ-101. The primary purpose of the mixer pump test (MPT) is to demonstrate that the two 300 horsepower mixer pumps installed in tank 241-AZ-101 can mobilize the settled sludge so that it can be retrieved for treatment and vitrification. Sampling will be performed in accordance with Tank 241-AZ-101 Mixer Pump Test Data Quality Objective (Banning 1999) and Data Quality Objectives for Regulatory Requirements for Hazardous and Radioactive Air Emissionsmore » Sampling and Analysis (Mulkey 1999). The sampling will verify if current air emission estimates used in the permit application are correct and provide information for future air permit applications.« less

Tank 241-AZ-101 Mixer Pump Test Vapor Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

TEMPLETON, A.M.

2000-01-31

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for vapor samples obtained during the operation of mixer pumps in tank 241-AZ-101. The primary purpose of the mixer pump test (MPT) is to demonstrate that the two 300 horsepower mixer pumps installed in tank 241-AZ-101 can mobilize the settled sludge so that it can be retrieved for treatment and vitrification Sampling will be performed in accordance with Tank 241-AZ-101 Mixer Pump Test Data Quality Objective (Banning 1999) and Data Quality Objectives for Regulatory Requirements for Hazardous and Radioactive Air Emissionsmore » Sampling and Analysis (Mulkey 1999). The sampling will verify if current air emission estimates used in the permit application are correct and provide information for future air permit applications.« less

Tank 241-AZ-101 Mixer Pump Test Vapor Sampling and Analysis Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

TEMPLETON, A.M.

2000-04-10

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for vapor samples obtained during the operation of mixer pumps in tank 241-AZ-101. The primary purpose of the mixer pump test (MPT) is to demonstrate that the two 300 horsepower mixer pumps installed in tank 241-AZ-101 can mobilize the settled sludge so that it can be retrieved for treatment and vitrification. Sampling will be performed in accordance with Tank 241-AZ-101 Mixer Pump Test Data Quality Objective (Banning 1999) and Data Quality Objectives for Regulatory Requirements for Hazardous and Radioactive Air Emissionsmore » Sampling and Analysis (Mulkey 1999). The sampling will verify if current air emission estimates used in the permit application are correct and provide information for future air permit applications.« less

Mechanical and Wear Properties of Sb- and Y-Added Mg-9Al-1Zn (AZ91) Alloy

NASA Astrophysics Data System (ADS)

Boby, Arun; Srinivasan, A.; Pillai, U. T. S.; Pai, B. C.

2015-09-01

This paper studies the effect of Sb and Y additions on the microstructure and mechanical properties of the AZ91 alloy. The results indicate that the Sb and Y additions lead to the formation of Mg3Sb2 and Al2Y phases. These phases modify the morphology of the β-Mg17Al12 phase, and hence refine the microstructure. The effects of Sb and Y additions on the aging behavior have also been investigated. Aging of the AZ91 alloy results in the formation of continuous and discontinuous types of precipitates. Whereas Sb and Y additions to AZ91 alloy suppresses the formation of discontinuous precipitate. The paper also reports the mechanical properties of as-cast and aged Sb-added AZ91-xY alloys for room and high temperatures. The optimum tensile properties are obtained with the alloy having the combined addition of 0.5 wt pct Sb and 0.6 wt pct Y. The fracture surface of AZ91-0.5Sb-0.6Y alloy reveals more quasi-cleavage type of failure with a cleavage fracture than the base alloy. At HT, the AZ91-0.5Sb-0.6Y alloy displays more cleavage facets connected with tearing ridges and shallow dimples than AZ91 alloy. Furthermore, it observed the improvement in wear resistance through the addition of Y. The worn surface reveals abrasion, oxidation, delamination, and plastic deformation wear mechanisms.

The influence of abscisic acid on the ethylene biosynthesis pathway in the functioning of the flower abscission zone in Lupinus luteus.

PubMed

Wilmowicz, Emilia; Frankowski, Kamil; Kućko, Agata; Świdziński, Michał; de Dios Alché, Juan; Nowakowska, Anna; Kopcewicz, Jan

2016-11-01

Flower abscission is a highly regulated developmental process activated in response to exogenous (e.g. changing environmental conditions) and endogenous stimuli (e.g. phytohormones). Ethylene (ET) and abscisic acid (ABA) are very effective stimulators of flower abortion in Lupinus luteus, which is a widely cultivated species in Poland, Australia and Mediterranean countries. In this paper, we show that artificial activation of abscission by flower removal caused an accumulation of ABA in the abscission zone (AZ). Moreover, the blocking of that phytohormone's biosynthesis by NDGA (nordihydroguaiaretic acid) decreased the number of abscised flowers. However, the application of NBD - an inhibitor of ET action - reversed the stimulatory effect of ABA on flower abscission, indicating that ABA itself is not sufficient to turn on the organ separation. Our analysis revealed that exogenous ABA significantly accelerated the transcriptional activity of the ET biosynthesis genes ACC synthase (LlACS) and oxidase (LlACO), and moreover, strongly increased the level of 1-aminocyclopropane-1-carboxylic acid (ACC) - ET precursor, which was specifically localized within AZ cells. We cannot exclude the possibility that ABA mediates flower abscission processes by enhancing the ET biosynthesis rate. The findings of our study will contribute to the overall basic knowledge on the phytohormone-regulated generative organs abscission in L. luteus. Copyright © 2016 Elsevier GmbH. All rights reserved.

Liprin-α3 controls vesicle docking and exocytosis at the active zone of hippocampal synapses.

PubMed

Wong, Man Yan; Liu, Changliang; Wang, Shan Shan H; Roquas, Aram C F; Fowler, Stephen C; Kaeser, Pascal S

2018-02-27

The presynaptic active zone provides sites for vesicle docking and release at central nervous synapses and is essential for speed and accuracy of synaptic transmission. Liprin-α binds to several active zone proteins, and loss-of-function studies in invertebrates established important roles for Liprin-α in neurodevelopment and active zone assembly. However, Liprin-α localization and functions in vertebrates have remained unclear. We used stimulated emission depletion superresolution microscopy to systematically determine the localization of Liprin-α2 and Liprin-α3, the two predominant Liprin-α proteins in the vertebrate brain, relative to other active-zone proteins. Both proteins were widely distributed in hippocampal nerve terminals, and Liprin-α3, but not Liprin-α2, had a prominent component that colocalized with the active-zone proteins Bassoon, RIM, Munc13, RIM-BP, and ELKS. To assess Liprin-α3 functions, we generated Liprin-α3-KO mice by using CRISPR/Cas9 gene editing. We found reduced synaptic vesicle tethering and docking in hippocampal neurons of Liprin-α3-KO mice, and synaptic vesicle exocytosis was impaired. Liprin-α3 KO also led to mild alterations in active zone structure, accompanied by translocation of Liprin-α2 to active zones. These findings establish important roles for Liprin-α3 in active-zone assembly and function, and suggest that interplay between various Liprin-α proteins controls their active-zone localization.

Review of Waste Management Symposium 2007, Tucson, AZ, USA

DOE PAGES

Luna, Robert E.; Yoshimura, R. H.

2007-03-01

The Waste Management Symposium 2007 is the most recent in a long series that has been held at Tucson, Arizona. The meeting has become extremely popular as a venue for technical exchange, marketing, and networking involving upward of 1800 persons involved with various aspects of radioactive waste management. However, in a break with tradition, the symposium organizers reported that next year’s Waste Management Symposium would be held at the Phoenix, AZ convention center. Additionally, most of the WM07 sessions dealt with the technical and institutional issues relating to the resolution of waste disposal and processing challenges, including a number ofmore » sessions dealing with related transport activities.« less

Transcriptional activation of a 37 kDa ethylene responsive cysteine protease gene, RbCP1, is associated with protein degradation during petal abscission in rose

PubMed Central

Tripathi, Siddharth Kaushal; Singh, Amar Pal; Sane, Aniruddha P.; Nath, Pravendra

2009-01-01

Cysteine proteases play an important role in several developmental processes in plants, particularly those related to senescence and cell death. A cysteine protease gene, RbCP1, has been identified that encodes a putative protein of 357 amino acids and is expressed in the abscission zone (AZ) of petals in rose. The gene was responsive to ethylene in petals, petal abscission zones, leaves, and thalamus. The expression of RbCP1 increased during both ethylene-induced as well as natural abscission and was inhibited by 1-MCP. Transcript accumulation of RbCP1 was accompanied by the appearance of a 37 kDa cysteine protease, a concomitant increase in protease activity and a substantial decrease in total protein content in the AZ of petals. Agro-injection of rose petals with a 2.0 kb region upstream of the RbCP1 gene could drive GUS expression in an abscission zone-specific manner and was blocked by 1-MCP. It is concluded that petal abscission is associated with a decrease in total protein content resulting from rapid transcription of RbCP1 and the expression of a 37 kDa protease. PMID:19346241

The Devils Mountain Fault zone: An active Cascadia upper plate zone of deformation, Pacific Northwest of North America

NASA Astrophysics Data System (ADS)

Barrie, J. Vaughn; Greene, H. Gary

2018-02-01

The Devils Mountain Fault Zone (DMFZ) extends east to west from Washington State to just south of Victoria, British Columbia, in the northern Strait of Juan de Fuca of Canada and the USA. Recently collected geophysical data were used to map this fault zone in detail, which show the main fault trace, and associated primary and secondary (conjugate) strands, and extensive northeast-southwest oriented folding that occurs within a 6 km wide deformation zone. The fault zone has been active in the Holocene as seen in the offset and disrupted upper Quaternary strata, seafloor displacement, and deformation within sediment cores taken close to the seafloor expression of the faults. Data suggest that the present DMFZ and the re-activated Leech River Fault may be part of the same fault system. Based on the length and previously estimated slip rates of the fault zone in Washington State, the DMFZ appears to have the potential of producing a strong earthquake, perhaps as large as magnitude 7.5 or greater, within 2 km of the city of Victoria.

Project W-211 Initial Tank Retrieval Systems (ITRS) Description of Operations for 241-AZ-102

DOE Office of Scientific and Technical Information (OSTI.GOV)

BRIGGS, S.R.

2000-02-25

The primary purpose of the Initial Tank Retrieval Systems (ITRS) is to provide systems for retrieval of radioactive wastes stored in underground double-shell tanks (DSTs) for transfer to alternate storage, evaporation, pretreatment or treatment, while concurrently reducing risks associated with safety watch list and other DSTs. This Description of Operation (DOO) defines the control philosophy for the waste retrieval system for Tank 241-AZ-102 (AZ-102). This DOO provides a basis for the detailed design of the Project W-211 Retrieval Control System (RCS) for AZ-102 and also establishes test criteria for the RCS.

Early Science with the Large Millimeter Telescope: CO and [C II] Emission in the z = 4.3 AzTEC J095942.9+022938 (COSMOS AzTEC-1)

NASA Astrophysics Data System (ADS)

Yun, Min S.; Aretxaga, I.; Gurwell, M. A.; Hughes, D. H.; Montaña, A.; Narayanan, G.; Rosa-González, D.; Sánchez-Argüelles, D.; Schloerb, F. P.; Snell, R. L.; Vega, O.; Wilson, G. W.; Zeballos, M.; Chavez, M.; Cybulski, R.; Díaz-Santos, T.; De La Luz, V.; Erickson, N.; Ferrusca, D.; Gim, H. B.; Heyer, M. H.; Iono, D.; Pope, A.; Rogstad, S. M.; Scott, K. S.; Souccar, K.; Terlevich, E.; Terlevich, R.; Wilner, D.; Zavala, J. A.

2015-12-01

Measuring redshifted CO line emission is an unambiguous method for obtaining an accurate redshift and total cold gas content of optically faint, dusty starburst systems. Here, we report the first successful spectroscopic redshift determination of AzTEC J095942.9+022938 (`COSMOS AzTEC-1'), the brightest 1.1 mm continuum source found in the AzTEC/James Clerk Maxwell Telescope survey (Scott et al.), through a clear detection of the redshifted CO (4-3) and CO (5-4) lines using the Redshift Search Receiver on the Large Millimeter Telescope. The CO redshift of z = 4.3420 ± 0.0004 is confirmed by the detection of the redshifted 158 μm [C II] line using the Submillimeter Array. The new redshift and Herschel photometry yield LFIR = (1.1 ± 0.1) × 1013 L⊙ and SFR ≈ 1300 M⊙ yr-1. Its molecular gas mass derived using the ultraluminous infrared galaxy conversion factor is 1.4 ± 0.2 × 1011M⊙ while the total interstellar medium mass derived from the 1.1 mm dust continuum is 3.7 ± 0.7 × 1011M⊙ assuming Td = 35 K. Our dynamical mass analysis suggests that the compact gas disc (r ≈ 1.1 kpc, inferred from dust continuum and spectral energy distribution analysis) has to be nearly face-on, providing a natural explanation for the uncommonly bright, compact stellar light seen by the HST. The [C II] line luminosity L_[C II]= 7.8± 1.1 × 10^9 L_{⊙} is remarkably high, but it is only 0.04 per cent of the total IR luminosity. AzTEC COSMOS-1 and other high redshift sources with a spatially resolved size extend the tight trend seen between [C II]/FIR ratio and ΣFIR among IR-bright galaxies reported by Díaz-Santos et al. by more than an order of magnitude, supporting the explanation that the higher intensity of the IR radiation field is responsible for the `[C II] deficiency' seen among luminous starburst galaxies.

ARSENIC REMOVAL FROM DRINKING WATER BY ADSORPTIVE MEDIA. USEPA DEMONSTRATION PROJECT AT VALLEY VISTA, AZ FINAL PERFORMANCE EVALUATION REPORT

EPA Science Inventory

This report documents the activities performed during and the results obtained from the arsenic removal treatment technology demonstration project at an Arizona Water Company (AWC) facility in Sedona, AZ, commonly referred to as Valley Vista. The objectives of the project were t...

Structural Analysis of Active North Bozgush Fault Zone (NW Iran)

NASA Astrophysics Data System (ADS)

Saber, R.; Isik, V.; Caglayan, A.

2013-12-01

NW Iran is one of the seismically active regions between Zagros Thrust Belt at the south and Caucasus at the north. Not only large magnitude historical earthquakes (Ms>7), but also 1987 Bozgush, 1997 Ardebil (Mw 6.1) and 2012 Ahar-Varzagan (Mw 6.4) earthquakes reveal that the region is seismically active. The North Bozgush Fault Zone (NBFZ) in this region has tens of kilometers in length and hundreds of meters in width. The zone has produced some large and destructive earthquakes (1593 M:6.1 and 1883 M:6.2). The NBFZ affects the Cenozoic units and along this zone Eocene units thrusted over Miocene and/or Plio-Quaternary sedimentary units. Together with morphologic features (stream offsets and alluvial fan movements) affecting the young unites reveal that the zone is active. The zone is mainly characterized by strike-slip faults with reverse component and reverse faults. Reverse faults striking N55°-85°E and dip of 40°-50° to the SW while strike-slip faults show right lateral slip with N60°-85°W and N60°-80°E directions. Our structural data analysis in NBFZ indicates that the axis direction of σ2 principal stress is vertical and the stress ratio (R) is 0.12. These results suggest that the tectonic regime along the North Bozgush Fault Zone is transpressive. Obtained other principal stresses (σ1, σ3) results are compatible with stress directions and GPS velocity suggested for NW Iran.

Active Ingredient - AZ

EPA Pesticide Factsheets

EPA Pesticide Chemical Search allows a user to easily find the pesticide chemical or active ingredient that they are interested in by using an array of simple to advanced search options. Chemical Search provides a single point of reference for easy access to information previously published in a variety of locations, including various EPA web pages and Regulations.gov.

Spontaneous and evoked release are independently regulated at individual active zones.

PubMed

Melom, Jan E; Akbergenova, Yulia; Gavornik, Jeffrey P; Littleton, J Troy

2013-10-30

Neurotransmitter release from synaptic vesicle fusion is the fundamental mechanism for neuronal communication at synapses. Evoked release following an action potential has been well characterized for its function in activating the postsynaptic cell, but the significance of spontaneous release is less clear. Using transgenic tools to image single synaptic vesicle fusion events at individual release sites (active zones) in Drosophila, we characterized the spatial and temporal dynamics of exocytotic events that occur spontaneously or in response to an action potential. We also analyzed the relationship between these two modes of fusion at single release sites. A majority of active zones participate in both modes of fusion, although release probability is not correlated between the two modes of release and is highly variable across the population. A subset of active zones is specifically dedicated to spontaneous release, indicating a population of postsynaptic receptors is uniquely activated by this mode of vesicle fusion. Imaging synaptic transmission at individual release sites also revealed general rules for spontaneous and evoked release, and indicate that active zones with similar release probability can cluster spatially within individual synaptic boutons. These findings suggest neuronal connections contain two information channels that can be spatially segregated and independently regulated to transmit evoked or spontaneous fusion signals.

Formation and mechanism of nanocrystalline AZ91 powders during HDDR processing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Yafen; Fan, Jianfeng, E-mail: fanjianfeng@tyu

2017-03-15

Grain sizes of AZ91 alloy powders were markedly refined to about 15 nm from 100 to 160 μm by an optimized hydrogenation-disproportionation-desorption-recombination (HDDR) process. The effect of temperature, hydrogen pressure and processing time on phase and microstructure evolution of AZ91 alloy powders during HDDR process was investigated systematically by X-ray diffraction, optical microscopy, scanning electron microscopy and transmission electron microscopy, respectively. The optimal HDDR process for preparing nanocrystalline Mg alloy powders is hydriding at temperature of 350 °C under 4 MPa hydrogen pressure for 12 h and dehydriding at 350 °C for 3 h in vacuum. A modified unreacted coremore » model was introduced to describe the mechanism of grain refinement of during HDDR process. - Highlights: • Grain size of the AZ91 alloy powders was significantly refined from 100 μm to 15 nm. • The optimal HDDR technology for nano Mg alloy powders is obtained. • A modified unreacted core model of grain refinement mechanism was proposed.« less

The effects of strain and stress state in hot forming of mg AZ31 sheet

NASA Astrophysics Data System (ADS)

Sherek, Paul A.; Carpenter, Alexander J.; Hector, Louis G.; Krajewski, Paul E.; Carter, Jon T.; Lasceski, Joshua; Taleff, Eric M.

Wrought magnesium alloys, such as AZ31 sheet, are of considerable interest for light-weighting of vehicle structural components. The poor room-temperature ductility of AZ31 sheet has been a hindrance to forming the complex part shapes necessary for practical applications. However, the outstanding formability of AZ31 sheet at elevated temperature provides an opportunity to overcome that problem. Complex demonstration components have already been produced at 450°C using gas-pressure forming. Accurate simulations of such hot, gas-pressure forming will be required for the design and optimization exercises necessary if this technology is to be implemented commercially. We report on experiments and simulations used to construct the accurate material constitutive models necessary for finite-element-method simulations. In particular, the effects of strain and stress state on plastic deformation of AZ31 sheet at 450°C are considered in material constitutive model development. Material models are validated against data from simple forming experiments.

AzTEC/ASTE 1.1 mm Deep Surveys: Number Counts and Clustering of Millimeter-bright Galaxies

NASA Astrophysics Data System (ADS)

Hatsukade, B.

2011-11-01

We present results of a 1.1 mm deep survey of the AKARI Deep Field South (ADF-S) with AzTEC mounted on the Atacama Submillimetre Telescope Experiment (ASTE). We obtained a map of 0.25 deg2 area with an rms noise level of 0.32-0.71 mJy. This is one of the deepest and widest maps thus far at millimetre and submillimetre wavelengths. We uncovered 198 sources with a significance of 3.5-15.6σ, providing the largest catalog of 1.1 mm sources in a contiguous region. Most of the sources are not detected in the far-infrared bands of the AKARI satellite, suggesting that they are mostly at z ≥ 1.5 given the detection limits. We construct differential and cumulative number counts of the ADF-S, the Subaru/XMM Newton Deep Field (SXDF), and the SSA 22 field surveyed by AzTEC/ASTE, which provide currently the tightest constraints on the faint end. The integration of the differential number counts of the ADF-S find that the contribution of 1.1 mm sources with ≥1 mJy to the cosmic infrared background (CIB) at 1.1 mm is 12-16%, suggesting that the large fraction of the CIB originates from faint sources of which number counts are not yet constrained. We estimate the cosmic star-formation rate density contributed by 1.1 mm sources with ≥1 mJy using the differential number counts and find that it is lower by about a factor of 5-10 compared to those derived from UV/optically-selected galaxies at z ~ 2-3. Clustering analyses of AzTEC sources in the ADF-S and the SXDF find that bright (>3 mJy) AzTEC sources are more strongly clustered than faint (< 3 mJy) AzTEC sources and the average mass of dark halos hosting bright AzTEC sources was calculated to be 1013-1014M⊙. Comparison of correlation length of AzTEC sources with other populations and with a bias evolution model suggests that dark halos hosting bright AzTEC sources evolve into systems of clusters at present universe and the AzTEC sources residing the dark halos evolve into massive elliptical galaxies located in the center of

DYNAMICS OF NASCENT AND ACTIVE ZONE ULTRASTRUCTURE AS SYNAPSES ENLARGE DURING LTP IN MATURE HIPPOCAMPUS

PubMed Central

Bell, Maria Elizabeth; Bourne, Jennifer N.; Chirillo, Michael A.; Mendenhall, John M.; Kuwajima, Masaaki; Harris, Kristen M.

2014-01-01

Nascent zones and active zones are adjacent synaptic regions that share a postsynaptic density, but nascent zones lack the presynaptic vesicles found at active zones. Here dendritic spine synapses were reconstructed through serial section electron microscopy (3DEM) and EM tomography to investigate nascent zone dynamics during long-term potentiation (LTP) in mature rat hippocampus. LTP was induced with theta-burst stimulation and comparisons were made to control stimulation in the same hippocampal slices at 5 minutes, 30 minutes, and 2 hours post-induction and to perfusion-fixed hippocampus in vivo. Nascent zones were present at the edges of ~35% of synapses in perfusion-fixed hippocampus and as many as ~50% of synapses in some hippocampal slice conditions. By 5 minutes, small dense core vesicles known to transport active zone proteins moved into more presynaptic boutons. By 30 minutes, nascent zone area decreased without significant change in synapse area, suggesting that presynaptic vesicles were recruited to pre-existing nascent zones. By 2 hours, both nascent and active zones were enlarged. Immunogold labeling revealed that glutamate receptors can be found in nascent zones; however, average distances from nascent zones to docked presynaptic vesicles ranged from 170±5 nm in perfusion-fixed hippocampus to 251±4 nm at enlarged synapses by 2 hours during LTP. Prior stochastic modeling suggests that falloff in glutamate concentration reduces the probability of glutamate receptor activation from 0.4 at the center of release to 0.1 just 200 nm away. Thus, conversion of nascent zones to functional active zones likely requires the recruitment of presynaptic vesicles during LTP. PMID:25043676

A Limited Dynamic Investigation of Magnesium Alloy AZ31B in 3 Orientations

DTIC Science & Technology

2016-09-01

YYYY) September 2016 2. REPORT TYPE Technical Report 3. DATES COVERED (From - To) 1 August 2014–31 December 2015 4. TITLE AND SUBTITLE A ...ARL-TR-7807 ● SEP 2016 US Army Research Laboratory A Limited Dynamic Investigation of Magnesium Alloy AZ31B in 3 Orientations...Laboratory A Limited Dynamic Investigation of Magnesium Alloy AZ31B in 3 Orientations by Tyrone L Jones Weapons and Materials Research Directorate

75 FR 81190 - Television Broadcasting Services; Yuma, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-27

... FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 [DA 10-2365; MB Docket No. 02-151; RM-10453] Television Broadcasting Services; Yuma, AZ AGENCY: Federal Communications Commission. ACTION: Dismissal... or via e-mail http://www.BCPIWEB.com . To request this document in accessible formats (computer...

78 FR 17743 - Navajo Nation Disaster #AZ-00026

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-22

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13515 and 13516] Navajo Nation Disaster AZ-00026 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the... INFORMATION CONTACT: A. Escobar, Office of Disaster Assistance, U.S. Small Business Administration, 409 3rd...

Development of mechanical properties in a CaO added AZ31 magnesium alloy processed by equal-channel angular pressing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bae, Seong-Hwan; Metal Forming Technology R&D Group, Korea Institute of Industrial Technology, Incheon 406-840; Jung, Ki Ho

Processing through the application of equal-channel angular pressing (ECAP) is recognized as one of the attractive severe plastic deformation techniques where the processed bulk metals generally achieve ultrafine-grained microstructure leading to improved physical characteristics and mechanical properties. Magnesium has received much attention to date for its lightweight, high strength and excellent elasticity. Mg alloys with addition of CaO is reported to provide the successful casting procedure without usage of greenhouse gas, SF{sub 6}, whereas it is generally used for preventing the oxidation of Mg during casting. In the present investigation, a CaO added AZ31 (AZ31-CaO) magnesium alloy was processed bymore » ECAP at elevated temepratures with a few steps of reduction which result in significant grain refinement to ~ 1.5 μm after 6 passes. Compression testing at room temperature demonstrated the AZ31-CaO alloy after ECAP showed enhanced yield strength more than the as-processed commercial AZ31 alloy while both alloys maintained ductility in spite of significant reduction in grain size. The improved strength in the AZ31-CaO alloy was attributed to the formation of fine Al{sub 2}Ca precipitates which experience breaking-up through ECAP and accelerate the microstructural refinement. Moreover, the preservation of ductility was attributed to the enhancement of strain hardening capability in the AZ31 alloy at room temperature. This study discusses the feasibility of using ECAP to improve both strength and ductility on magnesium alloys by applying the diagram describing the paradox of strength and ductility. - Highlights: • AZ31 and AZ31-CaO magnesium alloys were processed by ECAP up to 6 passes. • AZ31-CaO alloy after ECAP showed improved yield strength without losing ductility. • CaO in AZ31 forms fine Al{sub 2}Ca accelerating microstructural refinement during ECAP. • Feasibility of using ECAP was shown to improve both strength and ductility in Mg.« less

ARSENIC REMOVAL FROM DRINKING WATER BY ADSORPTIVE MEDIA USEPA DEMONSTRATION PROJECT AT VALLEY VISTA, AZ SIX-MONTH EVALUATION REPORT

EPA Science Inventory

This report documents the activities performed and the results obtained from the first six months of the EPA arsenic removal technology demonstration project at the Arizona Water Company (AWC) facility in Sedona, AZ, commonly referred to as Valley Vista. The main objective of the...

Fixed-bed column performances of azure-II and auramine-O adsorption by Pinus eldarica stalks activated carbon and its composite with zno nanoparticles: Optimization by response surface methodology based on central composite design.

PubMed

Jafari, Maryam; Rahimi, Mahmood Reza; Ghaedi, Mehrorang; Javadian, Hamedreza; Asfaram, Arash

2017-12-01

A continuous adsorption was used for removal of azure II (AZ II) and auramine O (AO) from aqueous solutions using Pinus eldarica stalks activated carbon (PES-AC) from aqueous solutions. The effects of initial dye concentration, flow rate, bed height and contact time on removal percentage of AO and AZ II were evaluated and optimized by central composite design (CCD) at optimum pH = 7.0. ZnO nanoparticles loaded on activated carbon were also used to remove AO and AZ II at pH = 7.0 and other optimum conditions. The breakthrough curves were obtained at different flow rates, initial dye concentrations and bed heights and the experimental data were fitted by Thomas, Adams-Bohart and Yoon-Nelson models. The main parameters of fixed-bed column including its adsorption capacity at breakthrough point (q b ), adsorption capacity at saturation point (q s ), mass transfer zone (MTZ), total removal percentage (R%), and empty bed contact time (EBCT) were calculated. The removal percentages calculated for AZ II and AO II were in the range of 51.6-61.1% and 40.6-61.6%, respectively. Bed adsorption capacity (N 0 ) and critical bed depth (Z 0 ) were obtained by BDST model. Copyright © 2017 Elsevier Inc. All rights reserved.

A New Technique for Precision Photometry Using Alt/Az Telescopes

NASA Astrophysics Data System (ADS)

Kirkaptrick, Colin; Stacey, Piper; Swift, Jonathan

2018-06-01

We present and test a new method for flat field calibration of images obtained on telescopes with altitude-azimuth (Alt-Az) mounts. Telescopes using Alt-Az mounts typically employ a field “de-rotator” to account for changing parallactic angles of targets observed across the sky, or for long exposures of a single target. This “de-rotation” results in a changing orientation of the telescope optics with respect to the camera. This, in turn, can result in a flat field that is a function of camera orientation due to, for example, vignetting. In order to account for these changes we develop and test a new flat field technique using the observations of known transiting exoplanets.

78 FR 60698 - Safety Zone, Lucas Oil Drag Boat Racing Series; Thompson Bay, Lake Havasu City, AZ.

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-02

...]26'51.99'' N, 114[deg]21'03.83'' W South Zone Line: 34[deg]27'07.99'' N, 114[deg]21'09.93'' W 34[deg]26'51.99'' N, 114[deg]21'03.83'' W This safety zone is necessary to ensure personnel and vessels...]21'09.93'' W 34[deg]26'51.99'' N, 114[deg]21'03.83'' W South Zone Line: 34[deg]27'07.99'' N, 114[deg...

Ratcheting Strain and Microstructure Evolution of AZ31B Magnesium Alloy under a Tensile-Tensile Cyclic Loading

PubMed Central

Wang, Denghui; Wang, Wenxian; Zhou, Jun; He, Xiuli; Dong, Peng; Zhang, Hongxia; Sun, Liyong

2018-01-01

In this paper, studies were conducted to investigate the deformation behavior and microstructure change in a hot-rolled AZ31B magnesium alloy during a tensile-tensile cyclic loading. The relationship between ratcheting effect and microstructure change was discussed. The ratcheting effect in the material during current tensile-tensile fatigue loading exceeds the material’s fatigue limit and the development of ratcheting strain in the material experienced three stages: initial sharp increase stage (Stage I); steady stage (Stage II); and final abrupt increase stage (Stage III). Microstructure changes in Stage I and Stage II are mainly caused by activation of basal slip system. The Extra Geometrically Necessary Dislocations (GNDs) were also calculated to discuss the relationship between the dislocation caused by the basal slip system and the ratcheting strain during the cyclic loading. In Stage III, both the basal slip and the {11−20} twins are found active during the crack propagation. The fatigue crack initiation in the AZ31B magnesium alloy is found due to the basal slip and the {11−20} tensile twins. PMID:29597278

Ratcheting Strain and Microstructure Evolution of AZ31B Magnesium Alloy under a Tensile-Tensile Cyclic Loading.

PubMed

Yan, Zhifeng; Wang, Denghui; Wang, Wenxian; Zhou, Jun; He, Xiuli; Dong, Peng; Zhang, Hongxia; Sun, Liyong

2018-03-28

In this paper, studies were conducted to investigate the deformation behavior and microstructure change in a hot-rolled AZ31B magnesium alloy during a tensile-tensile cyclic loading. The relationship between ratcheting effect and microstructure change was discussed. The ratcheting effect in the material during current tensile-tensile fatigue loading exceeds the material's fatigue limit and the development of ratcheting strain in the material experienced three stages: initial sharp increase stage (Stage I); steady stage (Stage II); and final abrupt increase stage (Stage III). Microstructure changes in Stage I and Stage II are mainly caused by activation of basal slip system. The Extra Geometrically Necessary Dislocations (GNDs) were also calculated to discuss the relationship between the dislocation caused by the basal slip system and the ratcheting strain during the cyclic loading. In Stage III, both the basal slip and the {11-20} twins are found active during the crack propagation. The fatigue crack initiation in the AZ31B magnesium alloy is found due to the basal slip and the {11-20} tensile twins.

Effects of thermomechanical treatments on the microstructures and mechanical properties of GTA-welded AZ31B magnesium alloy

NASA Astrophysics Data System (ADS)

Chu, Ya-jie; Chen, Jian; Li, Xiao-quan; Wu, Shen-qing; Yang, Zong-hui

2012-10-01

Thermomechanical treatments were carried out to improve the properties of AZ31B joints prepared by gas tungsten arc welding. The microstructures of the joints were studied by optical microscopy and scanning electron microscopy with energy-dispersive spectrometry. Tensile tests and hardness tests were performed to investigate the effects of thermomechanical treatments on the mechanical properties of the joints. It is found that the thermomechanical-treated joints show superior mechanical properties against the as-welded joints, and their ultimate tensile strength can reach more than 92% of the base material. This mainly attributes to the formation of fine equiaxed grains in the fusion zone. After thermomechanical treatments the dendrites are transformed to fine spherical grains, and the dendritic segregation can be effectively eliminated.

The effects of alloying elements on microstructures and mechanical properties of tungsten inert gas welded AZ80 magnesium alloys joint

NASA Astrophysics Data System (ADS)

Li, Hui; Zhang, Jiansheng; Ding, Rongrong

2017-11-01

The effects of alloying elements on the macrostructures, microstructures and tensile strength of AZ80 Mg alloy weldments were studied in the present study. The results indicate that with the decrease of Al element content of filler wire, the welding defects of seam are gradually eliminated and the β-Mg17Al12 phases at α-Mg boundaries are refined and become discontinuous, which are beneficial to the improvement of tensile strength. With AZ31 Mg alloy filler wire, the maximum tensile strength of AZ80 weldment is 220 MPa and fracture occurs at the welding seam of joint. It is experimentally proved that robust AZ80 Mg alloy joints can be obtained by tungsten inert gas (TIG) welding process with AZ31 Mg alloy filler wire. However, further study is required to improve the microstructures and reduce welding defects of joint in order to further improve the joining strength of AZ80 Mg alloy joint.

Differential effects of NAA and 2,4-D in reducing floret abscission in cestrum (Cestrum elegans) cut flowers are associated with their differential activation of Aux/IAA homologous genes.

PubMed

Abebie, Bekele; Lers, Amnon; Philosoph-Hadas, Sonia; Goren, Raphael; Riov, Joseph; Meir, Shimon

2008-01-01

A previous study showed that the relative effectiveness of 2,4-dichlorophenoxyacetic acid (2,4-D) compared with that of 1-naphthaleneacetic acid (NAA) in reducing floret bud abscission in cestrum (Cestrum elegans) cut flowers was due to its acropetal transport. The aim of the present study was to examine if the differential effect of these auxins on floret abscission is reflected in the expression of Aux/IAA genes in the floret abscission zone (AZ). cDNAs were isolated by PCR-based cloning from the floret AZ of auxin-treated cut flowers. The expression patterns of the cDNAs in various tissues and the effect of indole-3-acetic acid (IAA), applied with or without cycloheximide, on their expression in the floret AZ were examined by northern blot analysis. The regulation of transcript accumulation in the floret AZ in response to NAA or 2,4-D was measured by real-time PCR during auxin pulsing of cut flowers and vase life, concomitantly with floret abscission. Six isolated cDNAs were identified to represent Aux/IAA homologous genes, designated as Cestrum elegans (Ce)-IAA1 to Ce-IAA6. Four Ce-IAA genes were characterized as early auxin-responsive genes (ARGs), and two (Ce-IAA1 and Ce-IAA5) as late ARGs. Only Ce-IAA5 was AZ-specific in floret buds. A temporal regulation of Ce-IAA transcript levels in the floret AZ was found, with 2,4-D inducing higher expression levels than NAA in floret buds. These Ce-IAA expression levels were negatively correlated with floret abscission. The differential transport characteristics of NAA and 2,4-D in cestrum cut flowers were reflected in differential activation of the Ce-IAA genes identified in the floret AZ. Therefore, Aux/IAA genes can be used as molecular markers to measure auxin activity, which reflects free auxin level in the AZ. Two of the identified genes, Ce-IAA1 and Ce-IAA5, may also have a regulatory role in abscission.

15 CFR 400.49 - Monitoring and reviews of zone operations and activity.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Monitoring and reviews of zone operations and activity. 400.49 Section 400.49 Commerce and Foreign Trade Regulations Relating to Commerce... FOREIGN-TRADE ZONES BOARD Operation of Zones and Administrative Requirements § 400.49 Monitoring and...

15 CFR 400.49 - Monitoring and reviews of zone operations and activity.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Monitoring and reviews of zone operations and activity. 400.49 Section 400.49 Commerce and Foreign Trade Regulations Relating to Commerce... FOREIGN-TRADE ZONES BOARD Operation of Zones and Administrative Requirements § 400.49 Monitoring and...

The public health roots of zoning: in search of active living's legal genealogy.

PubMed

Schilling, Joseph; Linton, Leslie S

2005-02-01

Improvements in the built environment and changes in land-use policy are promising approaches to increasing physical activity among a largely sedentary population. Opportunities for walking and cycling as part of daily life are important to increasing physical activity and improving health. Yet, local zoning codes and related land-use regulations have made it difficult to create vibrant, mixed-use neighborhoods with well-connected streets and more compact development-the infrastructure necessary to support healthier rates of walking and cycling for transportation. To better understand the dynamic nature of land-use law and policy, and how policymakers might accomplish zoning reform to encourage more physically active environments, this paper traces the public health roots of zoning through a family tree of land-use legal doctrines. Zoning and public health laws evolved from the same legal ancestors-the common law of public nuisance and the expansion of state police powers, both premised on protection of the public's health. When the U.S. Supreme Court approved zoning in the 1926 case of Ambler Realty v. Village of Euclid, it nominally recognized the health basis of zoning. But it went on to craft a new legal rationale focused more on protection of property rights and residential neighborhoods. Since Euclid, court decisions have given little consideration to the public health roots of zoning. Given an emerging body of research demonstrating the importance of walking-friendly environments and the deference shown by the courts to the passage of zoning laws, the courts are likely to support policymakers as they move to change zoning systems conceived long ago. Legal, historical, and policy rationales support the modernization of zoning and land use policies that allow sensible mixes of land uses. Mixed land uses make walking an attractive alternative to driving and support a more physically active and healthy citizenry.

Fusion competent synaptic vesicles persist upon active zone disruption and loss of vesicle docking

PubMed Central

Wang, Shan Shan H.; Held, Richard G.; Wong, Man Yan; Liu, Changliang; Karakhanyan, Aziz; Kaeser, Pascal S.

2016-01-01

In a nerve terminal, synaptic vesicle docking and release are restricted to an active zone. The active zone is a protein scaffold that is attached to the presynaptic plasma membrane and opposed to postsynaptic receptors. Here, we generated conditional knockout mice removing the active zone proteins RIM and ELKS, which additionally led to loss of Munc13, Bassoon, Piccolo, and RIM-BP, indicating disassembly of the active zone. We observed a near complete lack of synaptic vesicle docking and a strong reduction in vesicular release probability and the speed of exocytosis, but total vesicle numbers, SNARE protein levels, and postsynaptic densities remained unaffected. Despite loss of the priming proteins Munc13 and RIM and of docked vesicles, a pool of releasable vesicles remained. Thus, the active zone is necessary for synaptic vesicle docking and to enhance release probability, but releasable vesicles can be localized distant from the presynaptic plasma membrane. PMID:27537483

Effect of Thermal History on Microstructures and Mechanical Properties of AZ31 Magnesium Alloy Prepared by Friction Stir Processing

PubMed Central

Chai, Fang; Zhang, Datong; Li, Yuanyuan

2014-01-01

Hot-rolled AZ31 (Mg-2.57Al-0.84Zn-0.32Mn, in mass percentage) magnesium alloy is subjected to friction stir processing in air (normal friction stir processing, NFSP) and under water (submerged friction stir processing, SFSP). Thermal history of the two FSP procedures is measured, and its effect on microstructures and mechanical properties of the experimental materials is investigated. Compared with NFSP, the peak temperature during SFSP is lower and the duration time at a high temperature is shorter due to the enhanced cooling effect of water. Consequently, SFSP results in further grain refinement, and the average grain size of the NFSP and SFSP specimens in the stir zone (SZ) are 2.9 μm and 1.3 μm, respectively. Transmission electron microscopy (TEM) examinations confirm that grain refinement is attributed to continuous dynamic recrystallization both for NFSP and SFSP. The average Vickers hardness in the SZ of the NFSP and SFSP AZ31 magnesium alloy are 76 HV and 87 HV. Furthermore, the ultimate tensile strength and the elongation of the SFSP specimen increase from 191 MPa and 31.3% in the NFSP specimen to 210 MPa and 50.5%, respectively. Both the NFSP and SFSP alloys fail through ductile fracture, but the dimples are much more obvious in the SFSP alloy. PMID:28788532

Morphometric and chronological behavior of 2.45 GHz microwave ablation zones for colorectal cancer metastases and hepatocellular carcinoma in the liver: preliminary report.

PubMed

Hickson, Guy; Patel, Nirav; King, Alexander; Breen, David

2016-08-01

Percutaneous microwave ablation (MWA) is increasingly utilized in the treatment of primary and secondary hepatic malignancy. As an in-situ treatment appreciation of any signs of recurrence is critical for improving long-term oncological outcomes. Volumetry has been recognized as having advantages over orthogonal measurements in the response assessment of malignant lesions. Our study set out to look at the normal involution of an ablation zone (AZ) both volumetrically and morphologically to see if this information might aid the detection of local tumor progression. Cases were identified retrospectively from our database of liver MWA. We identified 34 AZs in total, 18 AZs in 16 hepatocellular carcinoma (HCC) patients with cirrhosis on imaging grounds and 13 AZs in patients with metastatic colorectal cancer. How these AZs developed over time was analyzed both morphologically and quantitatively using Siemens Syngo Via post-processing software. We used the software to produce volume measurements and short axis orthogonal measurements. A baseline measurement was taken on the first <30 day post-ablation scan and the chronological changes were then plotted. We saw differences between the cirrhotic and non-cirrhotic patients both in terms of morphological and volumetric changes. 12/13 non-cirrhotic AZs had a volume of <50% of the baseline scan within the first year. The cirrhotic patients were less predictable, but 14/18 still shrunk to less than 50% of baseline volume in the first year. Orthogonal measurements were less useful in both groups. Qualitatively, there was initially a slightly less well-defined border to the AZ in the first 3 months, which became better defined over time and certainly over the first year of AZ involution. Volumetric analysis is a useful adjunct to conventional measurements and qualitative analysis of AZs. This can be reassuring when orthogonal measurements are static or difficult to interpret. Our preliminary data suggest that the normal

Thermal Microstructural Stability of AZ31 Magnesium after Severe Plastic Deformation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Young, John P.; Askari, Hesam A.; Hovanski, Yuri

2015-03-01

Both equal channel angular pressing and friction stir processing have the ability to refine the grain size of twin roll cast AZ31 magnesium and potentially improve its superplastic properties. This work used isochronal and isothermal heat treatments to investigate the microstructural stability of twin roll cast, equal channel angular pressed and friction stir processed AZ31 magnesium. For both heat treatment conditions, it was found that the twin roll casted and equal channel angular pressed materials were more stable than the friction stir processed material. Calculations of the grain growth kinetics showed that severe plastic deformation processing decreased the activation energymore » for grain boundary motion with the equal channel angular pressed material having the greatest Q value of the severely plastically deformed materials and that increasing the tool travel speed of the friction stir processed material improved microstructural stability. The Hollomon-Jaffe parameter was found to be an accurate means of identifying the annealing conditions that will result in substantial grain growth and loss of potential superplastic properties in the severely plastically deformed materials. In addition, Humphreys’s model of cellular microstructural stability accurately predicted the relative microstructural stability of the severely plastically deformed materials and with some modification, closely predicted the maximum grain size ratio achieved by the severely plastically deformed materials.« less

Evaluation of 241-AZ tank farm supporting phase 1 privatization waste feed delivery

DOE Office of Scientific and Technical Information (OSTI.GOV)

CARLSON, A.B.

1998-11-19

This evaluation is one in a series of evaluations determining the process needs and assessing the adequacy of existing and planned equipment in meeting those needs at various double-shell tank farms in support of Phase 1 privatization. A number of tank-to-tank transfers and waste preparation activities are needed to process and feed waste to the private contractor in support of Phase 1 privatization. The scope of this evaluation is limited to process needs associated with 241-AZ tank farm during the Phase 1 privatization.

Laser Welding-Brazing of Immiscible AZ31B Mg and Ti-6Al-4V Alloys Using an Electrodeposited Cu Interlayer

NASA Astrophysics Data System (ADS)

Zhang, Zequn; Tan, Caiwang; Wang, Gang; Chen, Bo; Song, Xiaoguo; Zhao, Hongyun; Li, Liqun; Feng, Jicai

2018-03-01

Metallurgical bonding between immiscible system AZ31B magnesium (Mg) and Ti-6Al-4V titanium (Ti) was achieved by adding Cu interlayer using laser welding-brazing process. Effect of the laser power on microstructure evolution and mechanical properties of Mg/Cu-coated Ti joints was studied. Visually acceptable joints were obtained at the range of 1300 to 1500 W. The brazed interface was divided into three parts due to temperature gradient: direct irradiation zone, intermediate zone and seam head zone. Ti3Al phase was produced along the interface at the direct irradiation zone. Ti-Al reaction layer grew slightly with the increase in laser power. A small amount of Ti2(Cu,Al) interfacial compounds formed at the intermediate zone and the ( α-Mg + Mg2Cu) eutectic structure dispersed in the fusion zone instead of gathering when increasing the laser power at this zone. At the seam head zone, Mg-Cu eutectic structure was produced in large quantities under all cases. Joint strength first increased and then decreased with the variation of the laser power. The maximum fracture load of Mg/Cu-coated Ti joint reached 2314 N at the laser power of 1300 W, representing 85.7% joint efficiency when compared with Mg base metal. All specimens fractured at the interface. The feature of fracture surface at the laser power of 1100 W was characterized by overall smooth surface. Obvious tear ridge and Ti3Al particles were observed at the fracture surface with increase in laser power. It suggested atomic diffusion was accelerated with more heat input giving rise to the enhanced interfacial reaction and metallurgical bonding in direct irradiation zone, which determined the mechanical properties of the joint.

14 CFR Appendix to Subpart U of... - Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ

Code of Federal Regulations, 2012 CFR

2012-01-01

... Grand Canyon National Park, AZ Appendix to Subpart U of Part 93 Aeronautics and Space FEDERAL AVIATION... TRAFFIC RULES Special Flight Rules in the Vicinity of Grand Canyon National Park, AZ Pt. 93, Subpt. U, App. Appendix to Subpart U of Part 93—Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ...

14 CFR Appendix to Subpart U of... - Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ

Code of Federal Regulations, 2010 CFR

2010-01-01

... Grand Canyon National Park, AZ Appendix to Subpart U of Part 93 Aeronautics and Space FEDERAL AVIATION... TRAFFIC RULES Special Flight Rules in the Vicinity of Grand Canyon National Park, AZ Pt. 93, Subpt. U, App. Appendix to Subpart U of Part 93—Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ...

14 CFR Appendix to Subpart U of... - Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ

Code of Federal Regulations, 2013 CFR

2013-01-01

... Grand Canyon National Park, AZ Appendix to Subpart U of Part 93 Aeronautics and Space FEDERAL AVIATION... TRAFFIC RULES Special Flight Rules in the Vicinity of Grand Canyon National Park, AZ Pt. 93, Subpt. U, App. Appendix to Subpart U of Part 93—Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ...

14 CFR Appendix to Subpart U of... - Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ

Code of Federal Regulations, 2011 CFR

2011-01-01

... Grand Canyon National Park, AZ Appendix to Subpart U of Part 93 Aeronautics and Space FEDERAL AVIATION... TRAFFIC RULES Special Flight Rules in the Vicinity of Grand Canyon National Park, AZ Pt. 93, Subpt. U, App. Appendix to Subpart U of Part 93—Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ...

14 CFR Appendix to Subpart U of... - Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ

Code of Federal Regulations, 2014 CFR

2014-01-01

... Grand Canyon National Park, AZ Appendix to Subpart U of Part 93 Aeronautics and Space FEDERAL AVIATION... TRAFFIC RULES Special Flight Rules in the Vicinity of Grand Canyon National Park, AZ Pt. 93, Subpt. U, App. Appendix to Subpart U of Part 93—Special Flight Rules in the Vicinity of the Grand Canyon National Park, AZ...

78 FR 3877 - Radio Broadcasting Services; Peach Springs, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-17

... FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 [MB Docket No. 12-374; RM-11687; DA 12-2072] Radio Broadcasting Services; Peach Springs, AZ AGENCY: Federal Communications Commission. ACTION... on or before February 26, 2013. ADDRESSES: Federal Communications Commission, 445 12th Street SW...

Influence of Aluminum Content on Grain Refinement and Strength of AZ31 Magnesium GTA Weld Metal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Babu, N. Kishore; Cross, Carl E.

2012-06-28

The goal is to characterize the effect of Al content on AZ31 weld metal, the grain size and strength, and examine role of Al on grain refinement. The approach is to systematically vary the aluminum content of AZ31 weld metal, Measure average grain size in weld metal, and Measure cross-weld tensile properties and hardness. Conclusions are that: (1) increased Al content in AZ31 weld metal results in grain refinement Reason: higher undercooling during solidification; (2) weld metal grain refinement resulted in increased strength & hardness Reason: grain boundary strengthening; and (3) weld metal strength can be raised to wrought basemore » metal levels.« less

RGDC Peptide-Induced Biomimetic Calcium Phosphate Coating Formed on AZ31 Magnesium Alloy

PubMed Central

Cao, Lin; Wang, Lina; Fan, Lingying; Xiao, Wenjun; Lin, Bingpeng; Xu, Yimeng; Liang, Jun; Cao, Baocheng

2017-01-01

Magnesium alloys as biodegradable metal implants have received a lot of interest in biomedical applications. However, magnesium alloys have extremely high corrosion rates a in physiological environment, which have limited their application in the orthopedic field. In this study, calcium phosphate compounds (Ca–P) coating was prepared by arginine–glycine–aspartic acid–cysteine (RGDC) peptide-induced mineralization in 1.5 simulated body fluid (SBF) to improve the corrosion resistance and biocompatibility of the AZ31 magnesium alloys. The adhesion of Ca–P coating to the AZ31 substrates was evaluated by a scratch test. Corrosion resistance and cytocompatibility of the Ca–P coating were investigated. The results showed that the RGDC could effectively promote the nucleation and crystallization of the Ca–P coating and the Ca–P coating had poor adhesion to the AZ31 substrates. The corrosion resistance and biocompatibility of the biomimetic Ca–P coating Mg alloys were greatly improved compared with that of the uncoated sample. PMID:28772717

Ethylene-responsive genes are differentially regulated during abscission, organ senescence and wounding in peach (Prunus persica).

PubMed

Ruperti, Benedetto; Cattivelli, Luigi; Pagni, Silvana; Ramina, Angelo

2002-03-01

Ethylene-responsive genes from peach (Prunus persica, L. Batsch) were isolated by differential screening of a cDNA library constructed from abscission zones in which cell separation had been evoked by treatment with the ethylene analogue propylene. DNA and deduced protein sequences of four selected clones, termed Prunus persica Abscission zone (PpAz), revealed homology to thaumatin-like proteins (PpAz8 and PpAz44), to proteins belonging to the PR4 class of pathogenesis-related (PR) proteins (PpAz89), and to fungal and plant beta-D-xylosidases (PpAz152). Expression analyses conducted on embrioctomized and CEPA-treated fruitlets as well as on fruit explants have shown that PpAz8, PpAz44 and PpAz89 are preferentially transcribed in the cells of the fruit abscission zone rather than in the non-zone tissues. The PpAz152 transcript showed a different accumulation pattern being consistently and promptly induced by wounding and only slightly stimulated by propylene. By contrast, a complex pattern of transcript accumulation was found for the four genes in response to the wounding of leaves and during organ development and senescence. Based on this evidence, the existence of multiple regulatory pathways underlying the differential expression of the four PpAz genes in the different tissues and physiological processes is hypothesized.

AzTEC millimetre survey of the COSMOS field - I. Data reduction and source catalogue

NASA Astrophysics Data System (ADS)

Scott, K. S.; Austermann, J. E.; Perera, T. A.; Wilson, G. W.; Aretxaga, I.; Bock, J. J.; Hughes, D. H.; Kang, Y.; Kim, S.; Mauskopf, P. D.; Sanders, D. B.; Scoville, N.; Yun, M. S.

2008-04-01

We present a 1.1 mm wavelength imaging survey covering 0.3 deg2 in the COSMOS field. These data, obtained with the AzTEC continuum camera on the James Clerk Maxwell Telescope, were centred on a prominent large-scale structure overdensity which includes a rich X-ray cluster at z ~ 0.73. A total of 50 mm-galaxy candidates, with a significance ranging from 3.5 to 8.5σ, are extracted from the central 0.15 deg2 area which has a uniform sensitivity of ~1.3 mJybeam-1. 16 sources are detected with S/N >= 4.5, where the expected false-detection rate is zero, of which a surprisingly large number (9) have intrinsic (deboosted) fluxes >=5 mJy at 1.1 mm. Assuming the emission is dominated by radiation from dust, heated by a massive population of young, optically obscured stars, then these bright AzTEC sources have far-infrared luminosities >6 × 1012Lsolar and star formation rates >1100Msolaryr-1. Two of these nine bright AzTEC sources are found towards the extreme peripheral region of the X-ray cluster, whilst the remainder are distributed across the larger scale overdensity. We describe the AzTEC data reduction pipeline, the source-extraction algorithm, and the characterization of the source catalogue, including the completeness, flux deboosting correction, false-detection rate and the source positional uncertainty, through an extensive set of Monte Carlo simulations. We conclude with a preliminary comparison, via a stacked analysis, of the overlapping MIPS 24-μm data and radio data with this AzTEC map of the COSMOS field.

A biodegradable AZ91 magnesium alloy coated with a thin nanostructured hydroxyapatite for improving the corrosion resistance.

PubMed

Mukhametkaliyev, T M; Surmeneva, M A; Vladescu, A; Cotrut, C M; Braic, M; Dinu, M; Vranceanu, M D; Pana, I; Mueller, M; Surmenev, R A

2017-06-01

The main aim of this study was to investigate the properties of an AZ91 alloy coated with nanostructured hydroxyapatite (HA) prepared by radio frequency (RF) magnetron sputtering. The bioactivity and biomineralization of the AZ91 magnesium alloy coated with HA were investigated in simulated body fluid (SBF) via an in vitro test. Scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD) analyses were performed. The samples were immersed in SBF to study the ability of the surface to promote the formation of an apatite layer as well as corrosion resistance and mass change of the HA-coated AZ91 alloy. Electrochemical tests were performed to estimate the corrosion behaviour of HA-coated and uncoated samples. The results revealed the capability of the HA coating to significantly improve the corrosion resistance of the uncoated AZ91 alloy. Copyright © 2017 Elsevier B.V. All rights reserved.

78 FR 57545 - Proposed Establishment of Class D Airspace and Class E Airspace; Laguna AAF, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-19

... Air Field (AAF), (Yuma Proving Ground), Yuma, AZ. The establishment of an air traffic control tower... Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590; telephone (202) 366-9826... Proving Ground), Yuma, AZ, excluding R-2306E and R- 2307 when in effect; and Class E airspace extending...

Microstructure and Mechanical Properties of Fiber-Laser-Welded and Diode-Laser-Welded AZ31 Magnesium Alloy

NASA Astrophysics Data System (ADS)

Chowdhury, S. M.; Chen, D. L.; Bhole, S. D.; Powidajko, E.; Weckman, D. C.; Zhou, Y.

2011-07-01

The microstructures, tensile properties, strain hardening, and fatigue strength of fiber-laser-welded (FLW) and diode-laser-welded (DLW) AZ31B-H24 magnesium alloys were studied. Columnar dendrites near the fusion zone (FZ) boundary and equiaxed dendrites at the center of FZ, with divorced eutectic β-Mg17Al12 particles, were observed. The FLW joints had smaller dendrite cell sizes with a narrower FZ than the DLW joints. The heat-affected zone consisted of recrystallized grains. Although the DLW joints fractured at the center of FZ and exhibited lower yield strength (YS), ultimate tensile strength (UTS), and fatigue strength, the FLW joints failed at the fusion boundary and displayed only moderate reduction in the YS, UTS, and fatigue strength with a joint efficiency of ~91 pct. After welding, the strain rate sensitivity basically vanished, and the DLW joints exhibited higher strain-hardening capacity. Stage III hardening occurred after yielding in both base metal (BM) and welded samples. Dimple-like ductile fracture characteristics appeared in the BM, whereas some cleavage-like flat facets together with dimples and river marking were observed in the welded samples. Fatigue crack initiated from the specimen surface or near-surface defects, and crack propagation was characterized by the formation of fatigue striations along with secondary cracks.

Numerical Assessment of the Role of Slip and Twinning in Magnesium Alloy AZ31B During Loading Path Reversal

NASA Astrophysics Data System (ADS)

Wang, Huamiao; Wu, Peidong; Wang, Jian

2015-07-01

Magnesium alloy AZ31B plastically deforms via twinning and slip. Corresponding to the unidirectional nature of twinning, the activity of twinning/detwinning is directly related to loading history and materials texture. Using the elastic viscoplastic self-consistent model implementing with the twinning and detwinning model (EVPSC-TDT), we revisited experimental data of AZ31B sheets under four different strain paths: (1) tension-compression-tension along rolling direction, (2) tension-compression-tension along transverse direction, (3) compression-tension-compression along rolling direction, and (4) compression-tension-compression along transverse direction, and identified the dominant deformation mechanisms with respect to the strain path. We captured plastic deformation behaviors observed in experiments and quantitatively interpreted experimental observations in terms of the activities of different deformation mechanisms and the evolution of texture. It is found that the in-plane pre-tension has slight effect on the subsequent deformation, and the pre-compression and the reverse tension after compression have significant effect on the subsequent deformation. The inelastic behavior under compressive unloading is found to be insignificant at a small strain level but pronounced at a large strain level. Such significant effect is mainly ascribed to the activity of twinning and detwinning.

Development of Rolling Schedules for Equal Channel Angular Extrusion (ECAE)-Processed AZ31 Magnesium Alloy

DTIC Science & Technology

2016-04-01

Processed AZ31 Magnesium Alloy Sheet by Laszlo J Kecskes, Vincent H Hammond, Michael Eichhorst, Norman Herzig, and Lothar Meyer...Angular Extrusion (ECAE)–Processed AZ31 Magnesium Alloy Sheet by Laszlo J Kecskes and Vincent H Hammond Weapons and Materials Research...successfully reduced into 1.5-mm-thick sheets . Two sets of plates, each with a different texture type, were evaluated. Microscopic examination of

75 FR 76293 - Radio Broadcasting Services; Peach Springs, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-08

...] Radio Broadcasting Services; Peach Springs, AZ AGENCY: Federal Communications Commission. ACTION: Final... 281C3 at Peach Springs, Arizona, in order to maintain a first local service at that community. Channel... following reference coordinates: 35-33-46 North Latitude and 113-27-12 West Longitude. DATES: Effective...

Linking Plagioclase Zoning Patterns to Active Magma Processes

NASA Astrophysics Data System (ADS)

Izbekov, P. E.; Nicolaysen, K. P.; Neill, O. K.; Shcherbakov, V.; Eichelberger, J. C.; Plechov, P.

2016-12-01

Plagioclase, one of the most common and abundant mineral phases in volcanic products, will vary in composition in response to changes in temperature, pressure, composition of the ambient silicate melt, and melt H2O concentration. Changes in these parameters may cause dissolution or growth of plagioclase crystals, forming characteristic textural and compositional variations (zoning patterns), the complete core-to-rim sequence of which describes events experienced by an individual crystal from its nucleation to the last moments of its growth. Plagioclase crystals in a typical volcanic rock may look drastically dissimilar despite their spatial proximity and the fact that they have erupted together. Although they shared last moments of their growth during magma ascent and eruption, their prior experiences could be very different, as plagioclase crystals often come from different domains of the same magma system. Distinguishing similar zoning patterns, correlating them across the entire population of plagioclase crystals, and linking these patterns to specific perturbations in the magmatic system may provide additional perspective on the variety, extent, and timing of magma processes at active volcanic systems. Examples of magma processes, which may be distinguished based on plagioclase zoning patterns, include (1) cooling due to heat loss, (2) heating and/or pressure build up due to an input of new magmatic material, (3) pressure drop in response to magma system depressurization, and (4) crystal transfer between different magma domains/bodies. This review will include contrasting examples of zoning patters from recent eruptions of Augustine and Cleveland Volcanoes in Alaska, Sakurajima Volcano in Japan, Karymsky, Bezymianny, and Tolbachik Volcanoes in Kamchatka, as well as from the drilling into an active magma body at Krafla, Iceland.

Linking Plagioclase Zoning Patterns to Active Magma Processes

NASA Astrophysics Data System (ADS)

Izbekov, P. E.; Nicolaysen, K. P.; Neill, O. K.; Shcherbakov, V.; Plechov, P.; Eichelberger, J. C.

2015-12-01

Plagioclase, one of the most common and abundant mineral phases in volcanic products, will vary in composition in response to changes in temperature, pressure, composition of the ambient silicate melt, and melt H2O concentration. Changes in these parameters may cause dissolution or growth of plagioclase crystals, forming characteristic textural and compositional variations (zoning patterns), the complete core-to-rim sequence of which describes events experienced by an individual crystal from its nucleation to the last moments of its growth. Plagioclase crystals in a typical volcanic rock may look drastically dissimilar despite their spatial proximity and the fact that they have erupted together. Although they shared last moments of their growth during magma ascent and eruption, their prior experiences could be very different, as plagioclase crystals often come from different domains of the same magma system. Distinguishing similar zoning patterns, correlating them across the entire population of plagioclase crystals, and linking these patterns to specific perturbations in the magmatic system may provide additional perspective on the variety, extent, and timing of magma processes at active volcanic systems. Examples of magma processes, which may be distinguished based on plagioclase zoning patterns, include (1) cooling due to heat loss, (2) heating and/or pressure build up due to an input of new magmatic material, (3) pressure drop in response to magma system depressurization, and (4) crystal transfer between different magma domains/bodies. This review will include contrasting examples of zoning patters from recent eruptions of Karymsky, Bezymianny, and Tolbachik Volcanoes in Kamchatka, Augustine and Cleveland Volcanoes in Alaska, as well as from the drilling into an active magma body at Krafla, Iceland.

Influence of Sulfate-Reducing Bacteria on the Corrosion Residual Strength of an AZ91D Magnesium Alloy

PubMed Central

Zhu, Xianyong; Liu, Yaohui; Wang, Qiang; Liu, Jiaan

2014-01-01

In this paper, the corrosion residual strength of the AZ91D magnesium alloy in the presence of sulfate-reducing bacteria is studied. In the experiments, the chemical composition of corrosion film was analyzed by a scanning electron microscope with energy dispersive X-ray spectroscopy. In addition, a series of instruments, such as scanning electronic microscope, pH-meter and an AG-10TA materials test machine, were applied to test and record the morphology of the corrosion product, fracture texture and mechanical properties of the AZ91D magnesium alloy. The experiments show that the sulfate-reducing bacteria (SRB) play an important role in the corrosion process of the AZ91D magnesium alloy. Pitting corrosion was enhanced by sulfate-reducing bacteria. Corrosion pits are important defects that could lead to a significant stress concentration in the tensile process. As a result, sulfate-reducing bacteria influence the corrosion residual strength of the AZ91D magnesium alloy by accelerating pitting corrosion. PMID:28788236

Ductility Improvement of an AZ61 Magnesium Alloy through Two-Pass Submerged Friction Stir Processing

PubMed Central

Luo, Xicai; Cao, Genghua; Zhang, Wen; Qiu, Cheng; Zhang, Datong

2017-01-01

Friction stir processing (FSP) has been considered as a novel technique to refine the grain size and homogenize the microstructure of metallic materials. In this study, two-pass FSP was conducted under water to enhance the cooling rate during processing, and an AZ61 magnesium alloy with fine-grained and homogeneous microstructure was prepared through this method. Compared to the as-cast material, one-pass FSP resulted in grain refinement and the β-Mg17Al12 phase was broken into small particles. Using a smaller stirring tool and an overlapping ratio of 100%, a finer and more uniform microstructure with an average grain size of 4.6 μm was obtained through two-pass FSP. The two-pass FSP resulted in a significant improvement in elongation of 37.2% ± 4.3%, but a slight decrease in strength compared with one-pass FSP alloy. Besides the microstructure refinement, the texture evolution in the stir zone is also considered responsible for the ductility improvement. PMID:28772614

Tank 241-AZ-101 criticality assessment resulting from pump jet mixing: Sludge mixing simulation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Onishi, Y.; Recknagle, K.

Tank 241-AZ-101 (AZ-101) is one of 28 double-shell tanks located in the AZ farm in the Hanford Site`s 200 East Area. The tank contains a significant quantity of fissile materials, including an estimated 9.782 kg of plutonium. Before beginning jet pump mixing for mitigative purposes, the operations must be evaluated to demonstrate that they will be subcritical under both normal and credible abnormal conditions. The main objective of this study was to address a concern about whether two 300-hp pumps with four rotating 18.3-m/s (60-ft/s) jets can concentrate plutonium in their pump housings during mixer pump operation and cause amore » criticality. The three-dimensional simulation was performed with the time-varying TEMPEST code to determine how much the pump jet mixing of Tank AZ-101 will concentrate plutonium in the pump housing. The AZ-101 model predicted that the total amount of plutonium within the pump housing peaks at 75 g at 10 simulation seconds and decreases to less than 10 g at four minutes. The plutonium concentration in the entire pump housing peaks at 0.60 g/L at 10 simulation seconds and is reduced to below 0.1 g/L after four minutes. Since the minimum critical concentration of plutonium is 2.6 g/L, and the minimum critical plutonium mass under idealized plutonium-water conditions is 520 g, these predicted maximums in the pump housing are much lower than the minimum plutonium conditions needed to reach a criticality level. The initial plutonium maximum of 1.88 g/L still results in safety factor of 4.3 in the pump housing during the pump jet mixing operation.« less

78 FR 20091 - Foreign-Trade Zone 26-Atlanta, Georgia, Authorization of Production Activity, Perkins Shibaura...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-03

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-90-2012] Foreign-Trade Zone 26--Atlanta, Georgia, Authorization of Production Activity, Perkins Shibaura Engines, LLC (Diesel Engines), Griffin, Georgia On November 29, 2012, Georgia Foreign-Trade Zone, Inc., grantee of FTZ 26, submitted a notification of proposed production activity to the...

Galaxy evolution at high-redshift: Millimeter-wavelength surveys with the AzTEC camera

NASA Astrophysics Data System (ADS)

Scott, Kimberly S.

Galaxies detected by their thermal dust emission at submillimeter (submm) and millimeter (mm) wavelengths comprise a population of massive, intensely star-forming systems in the early Universe. These "submm/mm- galaxies", or SMGs, likely represent an important phase in the assembly and/or evolution of massive galaxies and are thought to be the progenitors of massive elliptical galaxies. While their projected number density as a function of source brightness provides key constraints on models of galaxy evolution, SMG surveys carried out over the past twelve years with the first generation of submm/mm-wavelength cameras have not imaged a large enough area to sufficient depths to provide the statistical power needed to discriminate between competing galaxy evolution scenarios. In this dissertation, we present the results from SMG surveys carried out over the past four years using the new sensitive mm-wavelength camera AzTEC. With the improved mapping speed of the AzTEC camera combined with dedicated telescope time devoted to deep, large-area extragalactic surveys, we have tripled both the area surveyed towards blank- fields (that is, regions with no known galaxy over-densities) at submm/mm wavelengths and the total number of detected SMGs. Here, we describe the properties and performance of the AzTEC instrument while operating on the James Clerk Maxwell Telescope (JCMT) and the Atacama Submillimeter Telescope Experiment (ASTE). We then present the results from two of the blank-field regions imaged with AzTEC: the JCMT/COSMOS field, which we discovered is over- dense in the number of very bright SMGs, and the ASTE survey of the Great Observatories Origins Deep-South field, which represents one of the deepest surveys ever carried out at submm/mm wavelengths. Finally, we combine the results from all of the blank-fields imaged with AzTEC while operating on the JCMT and the ASTE to calculate the most accurate measurements to date of the SMG number counts.

Clarinet (CLA-1), a novel active zone protein required for synaptic vesicle clustering and release

PubMed Central

Nelson, Jessica; Richmond, Janet E; Colón-Ramos, Daniel A; Shen, Kang

2017-01-01

Active zone proteins cluster synaptic vesicles at presynaptic terminals and coordinate their release. In forward genetic screens, we isolated a novel Caenorhabditis elegans active zone gene, clarinet (cla-1). cla-1 mutants exhibit defects in synaptic vesicle clustering, active zone structure and synapse number. As a result, they have reduced spontaneous vesicle release and increased synaptic depression. cla-1 mutants show defects in vesicle distribution near the presynaptic dense projection, with fewer undocked vesicles contacting the dense projection and more docked vesicles at the plasma membrane. cla-1 encodes three isoforms containing common C-terminal PDZ and C2 domains with homology to vertebrate active zone proteins Piccolo and RIM. The C-termini of all isoforms localize to the active zone. Specific loss of the ~9000 amino acid long isoform results in vesicle clustering defects and increased synaptic depression. Our data indicate that specific isoforms of clarinet serve distinct functions, regulating synapse development, vesicle clustering and release. PMID:29160205

Active zone density is conserved during synaptic growth but impaired in aged mice

PubMed Central

Chen, Jie; Mizushige, Takafumi; Nishimune, Hiroshi

2013-01-01

Presynaptic active zones are essential structures for synaptic vesicle release, but the developmental regulation of their number and maintenance during aging at mammalian neuromuscular junctions (NMJs) remains unknown. Here, we analyzed the distribution of active zones in developing, mature, and aged mouse NMJs by immunohistochemical detection of the active zone-specific protein Bassoon. Bassoon is a cytosolic scaffolding protein essential for the active zone assembly in ribbon synapses and some brain synapses. Bassoon staining showed a punctate pattern in nerve terminals and axons at the nascent NMJ on embryonic days 16.5–18.5. Three-dimensional reconstruction of NMJs revealed that the majority of Bassoon puncta within an NMJ were attached to the presynaptic membrane from postnatal day 0 to adulthood, and colocalized with another active zone protein Piccolo. During postnatal development, the number of Bassoon puncta increased as the size of the synapses increased. Importantly, the density of Bassoon puncta remained relatively constant from postnatal day 0 to 54 at 2.3 puncta/μm2, while the synapse size increased 3.3-fold. However, Bassoon puncta density and signal intensity were significantly attenuated at the NMJs of 27-month-old aged mice. These results suggest that synapses maintain the density of synaptic vesicle release sites while the synapse size changes, but this density becomes impaired during aging. PMID:21935939

Effects of Yttrium Addition on Microstructure and Mechanical Properties of AZ80-2Sn Magnesium Alloys

NASA Astrophysics Data System (ADS)

Xue, Hansong; Yang, Gang; Li, Di; Xing, Zhihui; Pan, Fusheng

2015-12-01

The effects of Y on microstructure and mechanical properties of as-cast AZ80-2Sn magnesium alloys were investigated by optical microscopy, scanning electron microscopy and X-ray diffraction. Y addition not only changes the as-cast microstructure but also influences the mechanical properties of AZ80-2Sn alloy. Unmodified AZ80-2Sn alloys under casting state show that Mg17Al12 eutectic phase is semicontinuous and reticulated shape and distributes mainly at grain boundaries. Moreover, there are numerous Mg2Sn precipitate particles dispersing in Mg17Al12 eutectic phases. Y addition to as-cast AZ80-2Sn alloys has an important influence on the precipitation phase. But there has no obvious effect on grain refinement with Y addition. The results show that the AZ80-2Sn alloys with variable Y contents all contain Al2Y phase. By adding Y, the amount of Mg17Al12 is decreased and the dimension of that is reduced. Mg17Al12 eutectic phase turns to discontinuous, and the more disperse phases occur with the increase of Y content. The tensile tests indicate that a minor addition of Y can contribute to the formation of the dispersed small polygonal Al2Y particles and the improvement in the room-temperature strength. However, excessive Y addition leads to the coarsening of Al2Y phases, and thus results in the decline of strength and ductility.

Neurotransmitters couple brain activity to subventricular zone neurogenesis

PubMed Central

Young, Stephanie Z.; Taylor, M. Morgan; Bordey, Angélique

2011-01-01

Adult neurogenesis occurs in two privileged microenvironments, the hippocampal subgranular zone of the dentate gyrus and the subventricular zone (SVZ) along the lateral ventricle. This review focuses on accumulating evidence suggesting that the activity of specific brain regions or bodily states influences SVZ cell proliferation and neurogenesis. Neuromodulators such as dopamine and serotonin have been shown to have long-range effects through neuronal projections into the SVZ. Local GABA and glutamate signaling have demonstrated effects on SVZ proliferation and neurogenesis, but an extra-niche source of these neurotransmitters remains to be explored and options will be discussed. There is also accumulating evidence that diseases and bodily states such as Alzheimer's disease, seizures, sleep, and pregnancy influence SVZ cell proliferation. With such complex behavior and environmentally-driven factors that control subregion-specific activity, it will become necessary to account for overlapping roles of multiple neurotransmitter systems on neurogenesis when developing cell therapies or drug treatments. PMID:21395856

Simulation for Carbon Nanotube Dispersion and Microstructure Formation in CNTs/AZ91D Composite Fabricated by Ultrasonic Processing

NASA Astrophysics Data System (ADS)

Yang, Yuansheng; Zhao, Fuze; Feng, Xiaohui

2017-10-01

The dispersion of carbon nanotubes (CNTs) in AZ91D melt by ultrasonic processing and microstructure formation of CNTs/AZ91D composite were studied using numerical and physical simulations. The sound field and acoustic streaming were predicted using finite element method. Meanwhile, optimal immersion depth of the ultrasonic probe and suitable ultrasonic power were obtained. Single-bubble model was used to predict ultrasonic cavitation in AZ91D melt. The relationship between sound pressure amplitude and ultrasonic cavitation was established. Physical simulations of acoustic streaming and ultrasonic cavitation agreed well with the numerical simulations. It was confirmed that the dispersion of carbon nanotubes was remarkably improved by ultrasonic processing. Microstructure formation of CNTs/AZ91D composite was numerically simulated using cellular automation method. In addition, grain refinement was achieved and the growth of dendrites was changed due to the uniform dispersion of CNTs.

Dynamical Organization of Syntaxin-1A at the Presynaptic Active Zone

PubMed Central

Ullrich, Alexander; Böhme, Mathias A.; Schöneberg, Johannes; Depner, Harald; Sigrist, Stephan J.; Noé, Frank

2015-01-01

Synaptic vesicle fusion is mediated by SNARE proteins forming in between synaptic vesicle (v-SNARE) and plasma membrane (t-SNARE), one of which is Syntaxin-1A. Although exocytosis mainly occurs at active zones, Syntaxin-1A appears to cover the entire neuronal membrane. By using STED super-resolution light microscopy and image analysis of Drosophila neuro-muscular junctions, we show that Syntaxin-1A clusters are more abundant and have an increased size at active zones. A computational particle-based model of syntaxin cluster formation and dynamics is developed. The model is parametrized to reproduce Syntaxin cluster-size distributions found by STED analysis, and successfully reproduces existing FRAP results. The model shows that the neuronal membrane is adjusted in a way to strike a balance between having most syntaxins stored in large clusters, while still keeping a mobile fraction of syntaxins free or in small clusters that can efficiently search the membrane or be traded between clusters. This balance is subtle and can be shifted toward almost no clustering and almost complete clustering by modifying the syntaxin interaction energy on the order of only 1 kBT. This capability appears to be exploited at active zones. The larger active-zone syntaxin clusters are more stable and provide regions of high docking and fusion capability, whereas the smaller clusters outside may serve as flexible reserve pool or sites of spontaneous ectopic release. PMID:26367029

In vitro and in vivo evaluation of MgF2 coated AZ31 magnesium alloy porous scaffolds for bone regeneration.

PubMed

Yu, Weilin; Zhao, Huakun; Ding, Zhenyu; Zhang, Zhiwang; Sun, Benben; Shen, Ji; Chen, Shanshan; Zhang, Bingchun; Yang, Ke; Liu, Meixia; Chen, Daoyun; He, Yaohua

2017-01-01

Porous magnesium scaffolds are attracting increasing attention because of their degradability and good mechanical property. In this work, a porous and degradable AZ31 magnesium alloy scaffold was fabricated using laser perforation technique. To enhance the corrosion resistance and cytocompatibility of the AZ31 scaffolds, a fluoride treatment was used to acquire the MgF 2 coating. Enhanced corrosion resistance was confirmed by immersion and electrochemical tests. Due to the protection provided by the MgF 2 coating, the magnesium release and pH increase resulting from the degradation of the FAZ31 scaffolds were controllable. Moreover, in vitro studies revealed that the MgF 2 coated AZ31 (FAZ31) scaffolds enhanced the proliferation and attachment of rat bone marrow stromal cells (rBMSCs) compared with the AZ31 scaffolds. In addition, our present data indicated that the extract of the FAZ31 scaffold could enhance the osteogenic differentiation of rBMSCs. To compare the in vivo bone regenerative capacity of the AZ31 and FAZ31 scaffolds, a rabbit femoral condyle defect model was used. Micro-computed tomography (micro-CT) and histological examination were performed to evaluate the degradation of the scaffolds and bone volume changes. In addition to the enhanced the corrosion resistance, the FAZ31 scaffolds were more biocompatible and induced significantly more new bone formation in vivo. Conversely, bone resorption was observed from the AZ31 scaffolds. These promising results suggest potential clinical applications of the fluoride pretreated AZ31 scaffold for bone tissue repair and regeneration. Copyright © 2016 Elsevier B.V. All rights reserved.

Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope.

PubMed

Shi, Chun-Lin; Butenko, Melinka A

2018-01-01

Scanning electron microscope (SEM) is a type of electron microscope which produces detailed images of surface structures. It has been widely used in plants and animals to study cellular structures. Here, we describe a detailed protocol to prepare samples of floral abscission zones (AZs) for SEM, as well as further image analysis. We show that it is a powerful tool to detect morphologic changes at the cellular level during the course of abscission in wild-type plants and to establish the details of phenotypic alteration in abscission mutants.

In vitro corrosion of magnesium alloy AZ31 — a synergetic influence of glucose and Tris

NASA Astrophysics Data System (ADS)

Li, Ling-Yu; Liu, Bin; Zeng, Rong-Chang; Li, Shuo-Qi; Zhang, Fen; Zou, Yu-Hong; Jiang, Hongwei George; Chen, Xiao-Bo; Guan, Shao-Kang; Liu, Qing-Yun

2018-05-01

Biodegradable Mg alloys have generated great interest for biomedical applications. Accurate predictions of in vivo degradation of Mg alloys through cost-effective in vivo evaluations require the latter to be conducted in an environment close to that of physiological scenarios. However, the roles of glucose and buffering agents in regulating the in vivo degradation performance of Mg alloys has not been elucidated. Herein, degradation behavior of AZ31 alloy is investigated by hydrogen evolution measurements, pH monitoring and electrochemical tests. Results indicate that glucose plays a content-dependent role in degradation of AZ31 alloy in buffer-free saline solution. The presence of a low concentration of glucose, i.e. 1.0 g/L, decreases the corrosion rate of Mg alloy AZ31, whereas the presence of 2.0 and 3.0 g/L glucose accelerates the corrosion rate during long term immersion in saline solution. In terms of Tris-buffered saline solution, the addition of glucose increases pH value and promotes pitting corrosion or general corrosion of AZ31 alloy. This study provides a novel perspective to understand the bio-corrosion of Mg alloys in buffering agents and glucose containing solutions.

In vitro corrosion of magnesium alloy AZ31 — a synergetic influence of glucose and Tris

NASA Astrophysics Data System (ADS)

Li, Ling-Yu; Liu, Bin; Zeng, Rong-Chang; Li, Shuo-Qi; Zhang, Fen; Zou, Yu-Hong; Jiang, Hongwei George; Chen, Xiao-Bo; Guan, Shao-Kang; Liu, Qing-Yun

2018-06-01

Biodegradable Mg alloys have generated great interest for biomedical applications. Accurate predictions of in vivo degradation of Mg alloys through cost-effective in vivo evaluations require the latter to be conducted in an environment close to that of physiological scenarios. However, the roles of glucose and buffering agents in regulating the in vivo degradation performance of Mg alloys has not been elucidated. Herein, degradation behavior of AZ31 alloy is investigated by hydrogen evolution measurements, pH monitoring and electrochemical tests. Results indicate that glucose plays a content-dependent role in degradation of AZ31 alloy in buffer-free saline solution. The presence of a low concentration of glucose, i.e. 1.0 g/L, decreases the corrosion rate of Mg alloy AZ31, whereas the presence of 2.0 and 3.0 g/L glucose accelerates the corrosion rate during long term immersion in saline solution. In terms of Tris-buffered saline solution, the addition of glucose increases pH value and promotes pitting corrosion or general corrosion of AZ31 alloy. This study provides a novel perspective to understand the bio-corrosion of Mg alloys in buffering agents and glucose containing solutions.

78 FR 56859 - Foreign-Trade Zone 267-Fargo, North Dakota; Authorization of Production Activity; CNH America...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-16

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-51-2013] Foreign-Trade Zone 267--Fargo, North Dakota; Authorization of Production Activity; CNH America, LLC, (Construction and Agricultural Equipment..., submitted a notification of proposed production activity to the Foreign-Trade Zones (FTZ) Board on behalf of...

AzTEC 1.1 mm OBSERVATIONS OF THE MBM12 MOLECULAR CLOUD

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, M. J.; Kim, S.; Youn, S.

2012-02-10

We present 1.1 mm observations of the dust continuum emission from the MBM12 high-latitude molecular cloud observed with the Astronomical Thermal Emission Camera (AzTEC) mounted on the James Clerk Maxwell Telescope on Mauna Kea, Hawaii. We surveyed 6.34 deg{sup 2} centered on MBM12, making this the largest area that has ever been surveyed in this region with submillimeter and millimeter telescopes. Eight secure individual sources were detected with a signal-to-noise ratio of over 4.4. These eight AzTEC sources can be considered to be real astronomical objects compared to the other candidates based on calculations of the false detection rate. Themore » distribution of the detected 1.1 mm sources or compact 1.1 mm peaks is spatially anti-correlated with that of the 100 {mu}m emission and the {sup 12}CO emission. We detected the 1.1 mm dust continuum emitting sources associated with two classical T Tauri stars, LkH{alpha}262 and LkH{alpha}264. Observations of spectral energy distributions (SEDs) indicate that LkH{alpha}262 is likely to be Class II (pre-main-sequence star), but there are also indications that it could be a late Class I (protostar). A flared disk and a bipolar cavity in the models of Class I sources lead to more complicated SEDs. From the present AzTEC observations of the MBM12 region, it appears that other sources detected with AzTEC are likely to be extragalactic and located behind MBM12. Some of these have radio counterparts and their star formation rates are derived from a fit of the SEDs to the photometric evolution of galaxies in which the effects of a dusty interstellar medium have been included.« less

Systematic understanding of corrosion behavior of plasma electrolytic oxidation treated AZ31 magnesium alloy using a mouse model of subcutaneous implant.

PubMed

Jang, Yongseok; Tan, Zongqing; Jurey, Chris; Collins, Boyce; Badve, Aditya; Dong, Zhongyun; Park, Chanhee; Kim, Cheol Sang; Sankar, Jagannathan; Yun, Yeoheung

2014-12-01

This study was conducted to identify the differences between corrosion rates, corrosion types, and corrosion products in different physiological environments for AZ31 magnesium alloy and plasma electrolytic oxidation (PEO) treated AZ31 magnesium alloy. In vitro and in vivo tests were performed in Hank's Balanced Salt Solution (HBSS) and mice for 12 weeks, respectively. The corrosion rates of both AZ31 magnesium alloy and PEO treated AZ31 magnesium alloy were calculated based on DC polarization curves, volume of hydrogen evolution, and the thickness of corrosion products formed on the surface. Micro X-ray computed tomography (Micro-CT), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD) were used to analyze morphological and chemical characterizations of corrosion products. The results show that there is more severe localized corrosion after in vitro test in HBSS; however, the thicknesses of corrosion products formed on the surface for AZ31 magnesium alloy and PEO treated AZ31 magnesium alloy in vivo were about 40% thicker than the thickness of corrosion products generated in vitro. The ratio of Ca and P (Ca/P) in the corrosion products also differed. The Ca deficient region and higher content of Al in corrosion product than AZ31 magnesium alloy were identified after in vivo test in contrast with the result of in vitro test. Copyright © 2014 Elsevier B.V. All rights reserved.

78 FR 48647 - Foreign-Trade Zone 225-Springfield, Missouri; Authorization of Production Activity; General...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-09

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-30-2013] Foreign-Trade Zone 225--Springfield, Missouri; Authorization of Production Activity; General Dynamics Ordnance and Tactical Systems Munitions...-Trade Zones (FTZ) Board on behalf of General Dynamics Ordnance and Tactical Systems Munitions Services...

78 FR 32086 - Establishment of Class E Airspace; Tuba City, AZ

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-29

... INFORMATION: History On March 19, 2013, the FAA published in the Federal Register a notice of proposed..., at the Tuba City VORTAC, Tuba City, AZ. This action aids in containing aircraft while in IFR...

78 FR 30269 - Foreign-Trade Zone 129-Bellingham, Washington; Authorization of Production Activity; T.C. Trading...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-22

... proposed production activity to the Foreign-Trade Zones (FTZ) Board on behalf of T.C. Trading Company, Inc... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-8-2013] Foreign-Trade Zone 129--Bellingham, Washington; Authorization of Production Activity; T.C. Trading Company, Inc. (Eyeglass Assembly and Kitting...

Implications of a localized zone of seismic activity near the Inner Piedmont-Blue Ridge boundary

DOE Office of Scientific and Technical Information (OSTI.GOV)

Douglas, S.; Powell, C.

1994-03-01

A small but distinct cluster of earthquake activity is located in Henderson County, NC, near the boundary of the Inner Piedmont and Blue Ridge physiographic provinces. Over twenty events have occurred within the cluster since 1776 and four had body-wave magnitudes exceeding 3.0. Average focal depth for instrumentally recorded events is 7.7 km. Epicenters plot within the Inner Piedmont, roughly 13 km from the surface expression of the Brevard fault zone. The reason for sustained earthquake activity in Henderson County is not known but the close spatial association of the events with the Brevard fault suggests a causal relationship. Themore » Brevard zone dips steeply to the SE and the events could be associated with the fault at depth. An even more intriguing possibility is that the events are associated with the intersection of the Brevard zone and the decollemont; this possibility is compatible with available information concerning the depth to the decollemont and the dip on the Brevard zone. An association of seismic activity with the Brevard zone at depth is supported by the presence of another small cluster of activity located in Rutherford County, NC. This cluster is located in the Inner Piedmont, roughly 30 km NE of the Henderson cluster and 16 km from the Brevard fault zone. Association of seismic activity with known faults is very rare in the eastern US and has implications for tectonic models and hazard evaluation. Additional research must be conducted to determine the feasibility that activity is associated with the Brevard zone.« less

[Characteristics of soil organic carbon and enzyme activities in soil aggregates under different vegetation zones on the Loess Plateau].

PubMed

Li, Xin; Ma, Rui-ping; An, Shao-shan; Zeng, Quan-chao; Li, Ya-yun

2015-08-01

In order to explore the distribution characteristics of organic carbon of different forms and the active enzymes in soil aggregates with different particle sizes, soil samples were chosen from forest zone, forest-grass zone and grass zone in the Yanhe watershed of Loess Plateau to study the content of organic carbon, easily oxidized carbon, and humus carbon, and the activities of cellulase, β-D-glucosidase, sucrose, urease and peroxidase, as well as the relations between the soil aggregates carbon and its components with the active soil enzymes were also analyzed. It was showed that the content of organic carbon and its components were in order of forest zone > grass zone > forest-grass zone, and the contents of three forms of organic carbon were the highest in the diameter group of 0.25-2 mm. The content of organic carbon and its components, as well as the activities of soil enzymes were higher in the soil layer of 0-10 cm than those in the 10-20 cm soil layer of different vegetation zones. The activities of cellulase, β-D-glucosidase, sucrose and urease were in order of forest zone > grass zone > forest-grass zone. The peroxidase activity was in order of forest zone > forest-grass zone > grass zone. The activities of various soil enzymes increased with the decreasing soil particle diameter in the three vegetation zones. The activities of cellulose, peroxidase, sucrose and urease had significant positive correlations with the contents of various forms of organic carbon in the soil aggregates.

Remediation System Evaluation, A-Z Automotive in West Milford, New Jersey

EPA Pesticide Factsheets

The A-Z Automotive site is a former gasoline retail outlet and automobile service station located on Union Valley Road between St. George Street and Lou Ann Boulevard in West Milford, Passaic County, New Jersey.

Interfacial microstructure and mechanical properties of Cf/AZ91D composites with TiO2 and PyC fiber coatings.

PubMed

Li, Shaolin; Qi, Lehua; Zhang, Ting; Ju, Luyan; Li, Hejun

2017-10-01

In spite of the effectiveness of the fiber coatings on interface modification of carbon fiber reinforced magnesium matrix composites, the cost and exclusive equipment for the coatings preparation are usually ignored during research work. In this paper, pyrolytic carbon (PyC) and TiO 2 were coated on carbon fiber surface to study the effects of fiber coatings on interfacial microstructure and mechanical properties of carbon fiber reinforced AZ91D composites (C f /AZ91D composites). It was indicated that both the two coatings could modify the interface and improve the mechanical properties of the composites. The ultimate tensile strength of the TiO 2 -C f /AZ91D and the PyC-C f /AZ91D composite were 333MPa and 400MPa, which were improved by 41.7% and 70.2% respectively, compared with the untreated-C f /AZ91D composite. The microstructure observation revealed that the strengthening of the composites relied on fiber integrity and moderate interfacial bonding. MgO nano-particles were generated at the interface due to the reaction of TiO 2 with Mg in the TiO 2 -C f /AZ91D composite. The volume expansion resulting from the reaction let to disordered intergranular films and crystal defects at the interface. The fibers were protected and the interfacial reaction was restrained by PyC coating in the PyC-C f /AZ91D composite. The principle to select the coating of fiber was proposed by comparing the effectiveness and cost of the coatings. Copyright © 2017 Elsevier Ltd. All rights reserved.

A cross-shear deformation for optimizing the strength and ductility of AZ31 magnesium alloys

PubMed Central

Hamad, Kotiba; Ko, Young Gun

2016-01-01

Magnesium alloys have recently attracted great interest due their lightweight and high specific strength. However, because of their hexagonal close-packed structure, they have few active slip systems, resulting in poor ductility and high mechanical anisotropy at room temperature. In the present work, we used a cross-shear deformation imposed by a differential speed rolling (DSR) technique to improve the room temperature strength and ductility of AZ31 magnesium alloy sheets. To introduce the cross-shear deformation, the sheets were rotated 180° around their longitudinal axis between the adjacent passes of DSR. The sheets of the AZ31 alloy subjected to the cross-shear deformation showed a uniform fine microstructure (1.2 ± 0.1 μm) with weak basal textures. The fabricated sheets showed a simultaneous high ultimate tensile strength and elongation-to-failure, i.e., ~333 MPa and ~21%, respectively. These were explained based on the structural features evolved due to the cross-shear deformation by DSR. The high strength was attributed to the uniform fine microstructure, whereas the high ductility was explained based on the basal texture weakening. PMID:27406685

Numerical assessment of the role of slip and twinning in magnesium alloy AZ31B during loading path reversal

DOE PAGES

Wang, Huamiao; Wu, Peidong; Wang, Jian

2015-04-17

Magnesium alloy AZ31B plastically deforms via twinning and slip. Corresponding to the unidirectional nature of twinning, the activity of twinning/detwinning is directly related to loading history and materials texture. Using the elastic viscoplastic self-consistent model implementing with the twinning and detwinning model (EVPSC–TDT), we revisited experimental data of AZ31B sheets under four different strain paths: (1) tension–compression–tension along rolling direction, (2) tension–compression–tension along transverse direction, (3) compression–tension–compression along rolling direction, and (4) compression–tension–compression along transverse direction, and identified the dominant deformation mechanisms with respect to the strain path. We captured plastic deformation behaviors observed in experiments and quantitatively interpreted experimentalmore » observations in terms of the activities of different deformation mechanisms and the evolution of texture. It is found that the in-plane pre-tension has slight effect on the subsequent deformation, and the pre-compression and the reverse tension after compression have significant effect on the subsequent deformation. The inelastic behavior under compressive unloading is found to be insignificant at a small strain level but pronounced at a large strain level. Lastly, such significant effect is mainly ascribed to the activity of twinning and detwinning.« less

Combined effects of cerium and cooling rate on microstructure and mechanical properties of AZ91 magnesium alloy

NASA Astrophysics Data System (ADS)

Cai, Huisheng; Guo, Feng; Su, Juan

2018-01-01

The specimens of AZ91-xCe(x = 0, 0.3, 0.6, 0.9, 1.2, mass fraction wt%) with different thicknesses were prepared by die casting process, their as-cast microstructure and room temperature mechanical properties were investigated to analyze the change rule of microstructure and mechanical properties of AZ91 magnesium alloy under combined effects of cooling rate and cerium content. The results show that, the microstructure and mechanical properties of AZ91 magnesium alloy were twofold influenced by cooling rate and cerium content. With the increase of cooling rate and Ce content, the average as-cast grain size is evidently refined; the amount of β-Mg17Al12 decreases and distribution becomes discrete. While decreasing cooling rate or increasing Ce content, Al4Ce phase is more and the morphology tends to strip and needle from granular and short rod-like. The tensile strength and elongation of AZ91-xCe magnesium alloy are improved with increasing cooling rate. With the increase of Ce content, the tensile strength and elongation of AZ91-xCe magnesium alloy increased first and decreased afterwards, besides the action of Ce to improve tensile strength and elongation is more evident under faster cooling rate. Mechanical properties of samples are optimal in this work, when Ce content is 0.96% and cooling rate is 39.6 K s-1, tensile strength (259.7 MPa) and elongation (5.5%) are reached maximum, respectively.

Molecular determinants of the olfactory receptor Olfr544 activation by azelaic acid.

PubMed

Thach, Trung Thanh; Hong, Yu-Jung; Lee, Sangho; Lee, Sung-Joon

2017-04-01

The mouse olfactory receptor Olfr544 is expressed in several non-olfactory tissues and has been suggested as a functional receptor regulating different signaling pathways. However, the molecular interaction between Olfr544 and its natural ligand, azelaic acid (AzA), remains poorly characterized, primarily due to difficulties in the heterologous expression of the receptor protein on the cell membrane and lack of entire protein structure. In this report, we describe the molecular determinants of Olfr544 activation by AzA. N-terminal lucy-flag-rho tag ensured the heterologous expression of Olfr544 on the Hana3A cell surface. Molecular modeling and docking combined with mutational analysis identified amino acid residues in the Olfr544 for the interaction with AzA. Our data demonstrated that the Y109 residue in transmembrane helix 3 forms a hydrogen bond with AzA, which is crucial for the receptor-ligand interaction and activation. Y109 is required for the Olfr544 activation by AzA which, in turn, stimulates the Olfr544-dependent CREB-PGC-1α signaling axis and is followed by the induction of mitochondrial biogenesis in Olfr544 wild-type transfected Hana3A cells, but not in mock or Y109A mutant transfected cells. Collectively, these data indicated that a hydrogen bond between Y109 residue and AzA is a major determinant of the Olfr544-AzA interaction and activation. Copyright © 2017 Elsevier Inc. All rights reserved.

77 FR 47429 - Agency Information Collection Activities; Petroleum Refineries in Foreign Trade Sub-zones

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-08

... Activities; Petroleum Refineries in Foreign Trade Sub-zones AGENCY: U.S. Customs and Border Protection (CBP... requirement concerning the Petroleum Refineries in Foreign Trade Sub-zones. This request for comment is being...: Title: Petroleum Refineries in Foreign Trade Sub-zones. OMB Number: 1651-0063. Form Number: None...

AzTEC Survey of the Central Molecular Zone: Modeling Dust SEDs and N-PDF with Hierarchical Bayesian Analysis

NASA Astrophysics Data System (ADS)

Tang, Yuping; Wang, Daniel; Wilson, Grant; Gutermuth, Robert; Heyer, Mark

2018-01-01

We present the AzTEC/LMT survey of dust continuum at 1.1mm on the central ˜ 200pc (CMZ) of our Galaxy. A joint SED analysis of all existing dust continuum surveys on the CMZ is performed, from 160µm to 1.1mm. Our analysis follows a MCMC sampling strategy incorporating the knowledge of PSFs in different maps, which provides unprecedented spacial resolution on distributions of dust temperature, column density and emissivity index. The dense clumps in the CMZ typically show low dust temperature ( 20K), with no significant sign of buried star formation, and a weak evolution of higher emissivity index toward dense peak. A new model is proposed, allowing for varying dust temperature inside a cloud and self-shielding of dust emission, which leads to similar conclusions on dust temperature and grain properties. We further apply a hierarchical Bayesian analysis to infer the column density probability distribution function (N-PDF), while simultaneously removing the Galactic foreground and background emission. The N-PDF shows a steep power-law profile with α > 3, indicating that formation of dense structures are suppressed.

Root Apex Transition Zone As Oscillatory Zone

PubMed Central

Baluška, František; Mancuso, Stefano

2013-01-01

Root apex of higher plants shows very high sensitivity to environmental stimuli. The root cap acts as the most prominent plant sensory organ; sensing diverse physical parameters such as gravity, light, humidity, oxygen, and critical inorganic nutrients. However, the motoric responses to these stimuli are accomplished in the elongation region. This spatial discrepancy was solved when we have discovered and characterized the transition zone which is interpolated between the apical meristem and the subapical elongation zone. Cells of this zone are very active in the cytoskeletal rearrangements, endocytosis and endocytic vesicle recycling, as well as in electric activities. Here we discuss the oscillatory nature of the transition zone which, together with several other features of this zone, suggest that it acts as some kind of command center. In accordance with the early proposal of Charles and Francis Darwin, cells of this root zone receive sensory information from the root cap and instruct the motoric responses of cells in the elongation zone. PMID:24106493

78 FR 68026 - Foreign-Trade Zone 32-Miami, Florida, Authorization of Production Activity, Almod Diamonds, Ltd...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-13

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-69-2013] Foreign-Trade Zone 32--Miami, Florida, Authorization of Production Activity, Almod Diamonds, Ltd. (Jewelry and Precious Stones), Miami, Florida On June 21, 2013, the Greater Miami Foreign-Trade Zone, Inc., grantee of FTZ 32, submitted a notification of proposed production activity to...

Experimental investigation of anisotropy evolution of AZ31 magnesium alloy sheets under tensile loading

NASA Astrophysics Data System (ADS)

Tari, D. Ghaffari; Worswick, M. J.

2011-05-01

Increasing demand for lighter final products has created new opportunities for the application of new light weight materials. Due to high strength to density ratio and good magnetic resistance properties, magnesium alloys are good candidates to replace steel and aluminum for same application. However, limited numbers of active slip deformation mechanisms, result in a decreased formability at room temperature. Furthermore, wrought magnesium alloys have an initial crystallographic texture, remained from the prior rolling operations, which makes them highly anisotropic. In this paper, tensile tests are performed at room temperature and 200° C at different strain rates and orientations relative to the rolling direction, including rolling, 30°, 45°, 60° and transverse orientation. The strain rates adopted for these experiments varied from 0.001 to 1.0. The testing results show the effect of temperature on the strain rate sensitivity of AZ31 sheets. The extent of deformation is continuously recorded using two separate high temperature extensometers. The results of testing show an increase in the r-values with the plastic deformation. The strain rate sensitivity of AZ31 increased as the temperature was elevated. At higher strain rates the measured r-values are larger and the slope of its evolution with the plastic strain is steeper.

(Sub)millimetre interferometric imaging of a sample of COSMOS/AzTEC submillimetre galaxies. I. Multiwavelength identifications and redshift distribution

NASA Astrophysics Data System (ADS)

Miettinen, O.; Smolčić, V.; Novak, M.; Aravena, M.; Karim, A.; Masters, D.; Riechers, D. A.; Bussmann, R. S.; McCracken, H. J.; Ilbert, O.; Bertoldi, F.; Capak, P.; Feruglio, C.; Halliday, C.; Kartaltepe, J. S.; Navarrete, F.; Salvato, M.; Sanders, D.; Schinnerer, E.; Sheth, K.

2015-05-01

We used the Plateau de Bure Interferometer (PdBI) to map a sample of 15 submillimetre galaxies (SMGs) in the COSMOS field at the wavelength of 1.3 mm. The target SMGs were originally discovered in the James Clerk Maxwell Telescope (JCMT)/AzTEC 1.1 mm continuum survey at S/N1.1 mm = 4-4.5. This paper presents, for the first time, interferometric millimetre-wavelength observations of these sources. The angular resolution of our observations, 1''&dotbelow;8, allowed us to accurately determine the positions of the target SMGs. Using a detection threshold of S/N1.3 mm> 4.5 regardless of multiwavelength counterpart association, and 4 AzTEC19. Three of our detected SMGs (AzTEC21, 27, and 28; which corresponds to 20%) are marginally resolved at our angular resolution, and these sources are found to have elongated or clumpy morphologies and/or multiple components. Using optical to near-infrared photometric redshifts, available spectroscopic redshifts, and redshifts estimated from the radio-to-submm spectral index we infer a median redshift of tilde{z}= 3.20 ± 0.25 for our sample. To study the overall multiplicity and redshift distribution of flux-limited samples of SMGs we combined these sources with the 15 brightest JCMT/AzTEC SMGs detected at 1.1 mm, AzTEC1-15, and studied previously. This constitutes a complete, flux- and S/N-limited 1.1-mm selected sample. We find that the median redshift for the 15 brightest JCMT/AzTEC SMGs (tilde{z}= 3.05 ± 0.44) is consistent with that for AzTEC16-30. This conforms to recent observational findings that SMGs do not exhibit any significant trend between the redshift and (sub)mm flux density. For the combined AzTEC1-30 sample we derive a median redshift of tilde{z}= 3.17 ± 0.27, consistent with previous results based on mm