Science.gov

Sample records for activities covered include

  1. Photovoltaic concentrator assembly with optically active cover

    DOEpatents

    Plesniak, Adam P

    2014-01-21

    A photovoltaic concentrator assembly that includes a housing that defines an internal volume and includes a rim, wherein the rim defines an opening into the internal volume, a photovoltaic cell positioned in the internal volume, and an optical element that includes an optically active body and a flange extending outward from the body, wherein the flange is sealingly engaged with the rim of the housing to enclose the internal volume.

  2. 21 CFR 1404.220 - Are any procurement contracts included as covered transactions?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Are any procurement contracts included as covered transactions? 1404.220 Section 1404.220 Food and Drugs OFFICE OF NATIONAL DRUG CONTROL POLICY GOVERNMENTWIDE... the consent of a(n) Office of National Drug Control Policy official. In that case, the...

  3. Climatic Benefit of Swiss Forest Cover Change: Including Albedo Change into Net Carbon Balance

    NASA Astrophysics Data System (ADS)

    Schwaab, J.; Lehning, M.; Bebi, P.

    2012-12-01

    Forests influence climate through physical, chemical and biological processes. It has been shown that warming caused by the comparatively low albedo of forests (albedo-effect), can reduce or even exceed cooling caused by carbon storage in forests (CO2-effect). Although warming caused by albedo and the amount of carbon storage depend on local characteristics, studies are lacking that investigate the combined local patterns of albedo and CO2-effect. Our study area, Switzerland, provides a variety of geographical features and thus the possibility to show how different geographical variables influence the two effects. We used the concept of radiative forcing to compare the effect of a changing albedo and a change in atmospheric CO2 concentration due to land cover change in the past. The change of forest cover was analysed over a period of 12 years based on aerial photographs. We estimate the albedo-effect by combining albedo data derived from the satellite sensor MODIS and data on snow cover derived from the satellite sensor AVHRR. Changes in carbon storage were calculated as differences in biomass and soil stocks of specific land cover classes. We found carbon storage induced cooling to be higher than albedo induced warming everywhere in Switzerland. However, especially in altitudes over 1200 m the albedo-effect reduced the benefits of carbon storage by more than 50%. In lower altitudes the albedo change was less important. The albedo-effect in altitudes above 1200 m was more relevant because of a more persistent snow-cover, a slightly higher global radiation and less additional carbon storage. The relevance of warming caused by an albedo change did not only depend on altitude, but also on the characteristics of forest cover change. While transitions from open land to open forest were accompanied by high albedo changes, the albedo change was only marginal if open forest turned into closed forest. Since snow cover has a large influence on the albedo effect, we included

  4. DEVELOPMENT OF A PRODUCT MODEL FOR CUT-AND-COVER TUNNELS INCLUDING DEGRADATIONS

    NASA Astrophysics Data System (ADS)

    Aruga, Takashi; Yabuki, Nobuyoshi; Arai, Yasushi

    Cut-and-Cover tunnels are constructed on site. The various conditions of environments and techniques of construction make a significant influence on the quality of the tunnel. It is extremely difficult to rebuild the tunnel even if a structural trouble is found once the construction is completed. Thus, suitable maintenance is needed to ensure the tunnel is in a healthy condition. To execute better maintenance, the information on design and construction of the tunnel is vital for inspection of degradation, estimation of occurrence factors and planning of repair or refurbishing works. In this paper, we developed a product model for representing cut-and-cover tunnels including degradations for effective information use in maintenance work. As its first step, we investigated the characteristics of cut-and-cover tunnels and degradations about reinforced concrete members and developed a conceptual model. Then, we implemented the conceptual product model by expanding Industry Foundation Classes (IFC). Finally, we verified the product model by applying it to a simple tunnel.

  5. 25 CFR 900.196 - Do covered services include the conduct of clinical studies and investigations and the provision...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... studies and investigations and the provision of emergency services, including the operation of emergency motor vehicles? 900.196 Section 900.196 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... Claims § 900.196 Do covered services include the conduct of clinical studies and investigations and...

  6. 25 CFR 900.196 - Do covered services include the conduct of clinical studies and investigations and the provision...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... studies and investigations and the provision of emergency services, including the operation of emergency motor vehicles? 900.196 Section 900.196 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... Claims § 900.196 Do covered services include the conduct of clinical studies and investigations and...

  7. 25 CFR 900.196 - Do covered services include the conduct of clinical studies and investigations and the provision...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... studies and investigations and the provision of emergency services, including the operation of emergency motor vehicles? 900.196 Section 900.196 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... Claims § 900.196 Do covered services include the conduct of clinical studies and investigations and...

  8. 25 CFR 900.196 - Do covered services include the conduct of clinical studies and investigations and the provision...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... studies and investigations and the provision of emergency services, including the operation of emergency motor vehicles? 900.196 Section 900.196 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... Claims § 900.196 Do covered services include the conduct of clinical studies and investigations and...

  9. 25 CFR 900.196 - Do covered services include the conduct of clinical studies and investigations and the provision...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... studies and investigations and the provision of emergency services, including the operation of emergency motor vehicles? 900.196 Section 900.196 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR... Claims § 900.196 Do covered services include the conduct of clinical studies and investigations and...

  10. The dust covering factor in active galactic nuclei

    NASA Astrophysics Data System (ADS)

    Stalevski, Marko; Ricci, Claudio; Ueda, Yoshihiro; Lira, Paulina; Fritz, Jacopo; Baes, Maarten

    2016-05-01

    The primary source of emission of active galactic nuclei (AGNs), the accretion disc, is surrounded by an optically and geometrically thick dusty structure (`the so-called dusty torus'). The infrared radiation emitted by the dust is nothing but a reprocessed fraction of the accretion disc emission, so the ratio of the torus to the AGN luminosity (Ltorus/LAGN) should corresponds to the fraction of the sky obscured by dust, i.e. the covering factor. We undertook a critical investigation of the Ltorus/LAGN as the dust covering factor proxy. Using state-of-the-art 3D Monte Carlo radiative transfer code, we calculated a grid of spectral energy distributions (SEDs) emitted by the clumpy two-phase dusty structure. With this grid of SEDs, we studied the relation between Ltorus/LAGN and the dust covering factor for different parameters of the torus. We found that in the case of type 1 AGNs the torus anisotropy makes Ltorus/LAGN underestimate low covering factors and overestimate high covering factors. In type 2 AGNs Ltorus/LAGN always underestimates covering factors. Our results provide a novel easy-to-use method to account for anisotropy and obtain correct covering factors. Using two samples from the literature, we demonstrated the importance of our result for inferring the obscured AGN fraction. We found that after the anisotropy is properly accounted for, the dust covering factors show very weak dependence on LAGN, with values in the range of ≈0.6-0.7. Our results also suggest a higher fraction of obscured AGNs at high luminosities than those found by X-ray surveys, in part owing to the presence of a Compton-thick AGN population predicted by population synthesis models.

  11. 41 CFR 60-250.20 - Covered employment activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Covered employment activities. 60-250.20 Section 60-250.20 Public Contracts and Property Management Other Provisions Relating to..., organizational structures, position descriptions, lines of progression, and seniority lists; (e) Leaves...

  12. 41 CFR 60-741.20 - Covered employment activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 1 2012-07-01 2009-07-01 true Covered employment activities. 60-741.20 Section 60-741.20 Public Contracts and Property Management Other Provisions Relating to..., organizational structures, position descriptions, lines of progression, and seniority lists; (e) Leaves...

  13. 41 CFR 60-300.20 - Covered employment activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Covered employment activities. 60-300.20 Section 60-300.20 Public Contracts and Property Management Other Provisions Relating to..., organizational structures, position descriptions, lines of progression, and seniority lists; (e) Leaves...

  14. 41 CFR 60-741.20 - Covered employment activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Covered employment activities. 60-741.20 Section 60-741.20 Public Contracts and Property Management Other Provisions Relating to..., organizational structures, position descriptions, lines of progression, and seniority lists; (e) Leaves...

  15. 41 CFR 60-300.20 - Covered employment activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 1 2012-07-01 2009-07-01 true Covered employment activities. 60-300.20 Section 60-300.20 Public Contracts and Property Management Other Provisions Relating to..., organizational structures, position descriptions, lines of progression, and seniority lists; (e) Leaves...

  16. 41 CFR 60-250.20 - Covered employment activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 1 2012-07-01 2009-07-01 true Covered employment activities. 60-250.20 Section 60-250.20 Public Contracts and Property Management Other Provisions Relating to..., organizational structures, position descriptions, lines of progression, and seniority lists; (e) Leaves...

  17. 41 CFR 60-300.20 - Covered employment activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., whether or not administered by the contractor; (g) Selection and financial support for training, including... absence to pursue training; (h) Activities sponsored by the contractor including social and...

  18. 41 CFR 60-741.20 - Covered employment activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., whether or not administered by the contractor; (g) Selection and financial support for training, including... absence to pursue training; (h) Activities sponsored by the contractor including social and...

  19. Integrated uncertainty assessment of hydrological responses due to land cover change in a large river basin including feedback effects

    NASA Astrophysics Data System (ADS)

    Kumar, R.; Samaniego-Eguiguren, L. E.; Coskun, M.

    2010-12-01

    The quantification of the uncertainty of changes in hydrological variables such as runoff, soil moisture, amongst others, due to future land cover and climate changes has been one of the topical research areas in hydrology. In this study we are concerned only with the consequences of land cover change and its uncertainty, although the climate change has relatively higher impact on hydrological processes is excluded here to better comprehend the effects of land cover change on hydrological process and vice-versa (i.e. the feedback from the local hydrological conditions on land cover change). The effects of land cover change is cumulative and may cause long lasting consequences. Uncertainties in such modeling exercises may arise from various sources including those from the hydrologic model parameterization and from the possible pathways of future change scenarios. In most studies, the latter part of uncertainty is quantified by running a hydrologic model with a few land cover change scenarios obtained independently or in uncoupled way from the hydrological conditions. As a result this framework neither account for the synergy and the feedback effects among subsystems nor allows to assess the full uncertainty. Here, we present a novel approach for an integrated assessment of the effects of land cover change on hydrological fluxes in a mesoscale river basin. An attempt was made to couple a distributed hydrologic model (mHM) with a stochastic land cover change model, which was able to generate a wide range of plausible future land cover change paths based on likely developments of socioeconomic conditions of a region and the feedbacks from the hydrologic model. These feedbacks were formulated as rules taking into account the local hydrologic conditions (e.g. soil moisture dryness index, inundation hazard index, etc.) that effect the transition probabilities of land cover change. The uncertainty due to hydrologic model parameterizations was accounted by several ``good

  20. The effect of coverings, including plastic bags and wraps, on mortality and morbidity in preterm and full-term neonates

    PubMed Central

    Oatley, H K; Blencowe, H; Lawn, J E

    2016-01-01

    Neonatal hypothermia is an important risk factor for mortality and morbidity, and is common even in temperate climates. We conducted a systematic review to determine whether plastic coverings, used immediately following delivery, were effective in reducing the incidence of mortality, hypothermia and morbidity. A total of 26 studies (2271 preterm and 1003 term neonates) were included. Meta-analyses were conducted as appropriate. Plastic wraps were associated with a reduction in hypothermia in preterm (⩽29 weeks; risk ratio (RR)=0.57; 95% confidence interval (CI) 0.46 to 0.71) and term neonates (RR=0.76; 95% CI 0.60 to 0.96). No significant reduction in neonatal mortality or morbidity was found; however, the studies were underpowered for these outcomes. For neonates, especially preterm, plastic wraps combined with other environmental heat sources are effective in reducing hypothermia during stabilization and transfer within hospital. Further research is needed to quantify the effects on mortality or morbidity, and investigate the use of plastic coverings outside hospital settings or without additional heat sources. PMID:27109095

  1. The effect of coverings, including plastic bags and wraps, on mortality and morbidity in preterm and full-term neonates.

    PubMed

    Oatley, H K; Blencowe, H; Lawn, J E

    2016-05-01

    Neonatal hypothermia is an important risk factor for mortality and morbidity, and is common even in temperate climates. We conducted a systematic review to determine whether plastic coverings, used immediately following delivery, were effective in reducing the incidence of mortality, hypothermia and morbidity. A total of 26 studies (2271 preterm and 1003 term neonates) were included. Meta-analyses were conducted as appropriate. Plastic wraps were associated with a reduction in hypothermia in preterm (⩽29 weeks; risk ratio (RR)=0.57; 95% confidence interval (CI) 0.46 to 0.71) and term neonates (RR=0.76; 95% CI 0.60 to 0.96). No significant reduction in neonatal mortality or morbidity was found; however, the studies were underpowered for these outcomes. For neonates, especially preterm, plastic wraps combined with other environmental heat sources are effective in reducing hypothermia during stabilization and transfer within hospital. Further research is needed to quantify the effects on mortality or morbidity, and investigate the use of plastic coverings outside hospital settings or without additional heat sources.

  2. 30 CFR 580.4 - What activities are not covered by this part?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... regulations at 30 CFR parts 582 and 282; and (d) G&G exploration or G&G scientific research activities related to oil, gas, and sulphur, including gas hydrates, which are covered by regulations at 30 CFR parts...? 580.4 Section 580.4 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF THE...

  3. 30 CFR 580.4 - What activities are not covered by this part?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... regulations at 30 CFR parts 582 and 282; and (d) G&G exploration or G&G scientific research activities related to oil, gas, and sulphur, including gas hydrates, which are covered by regulations at 30 CFR parts...? 580.4 Section 580.4 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF THE...

  4. 30 CFR 580.4 - What activities are not covered by this part?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... regulations at 30 CFR parts 582 and 282; and (d) G&G exploration or G&G scientific research activities related to oil, gas, and sulphur, including gas hydrates, which are covered by regulations at 30 CFR parts...? 580.4 Section 580.4 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF THE...

  5. Everglades National Park Including Biscayne National Park. Activity Book.

    ERIC Educational Resources Information Center

    Ruehrwein, Dick

    Intended to help elementary school children learn about the resources of the Everglades and Biscayne National Parks, this activity book includes information, puzzles, games, and quizzes. The booklet deals with concepts related to: (1) the seasons; (2) fire ecology; (3) water; (4) fish; (5) mammals; (6) mosquitos; (7) birds; (8) venomous snakes;…

  6. Microbial community structure and abundance in the rhizosphere and bulk soil of a tomato cropping system that includes cover crops

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this report we use Terminal Restriction Fragment Length Polymorphisms (TRFLP) in a tomato production system to “finger printing” the soil microbial community structure with Phylum specific primer sets. Factors influencing the soil microbes are a cover crop of Hairy Vetch (Vicia villosa) or Rye (...

  7. 29 CFR 776.28 - Covered preparatory activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... company which was engaged in preliminary oil well drilling, even though the holes were drilled to a... drilling operations even though no oil was discovered. 27 Laborers employed in erecting drilling rigs would also be covered. 28 Other preparatory work before drilling begins in an oil field, such as staking...

  8. Determining the Covering Factor of Compton-thick Active Galactic Nuclei with NuSTAR

    NASA Astrophysics Data System (ADS)

    Brightman, M.; Baloković, M.; Stern, D.; Arévalo, P.; Ballantyne, D. R.; Bauer, F. E.; Boggs, S. E.; Craig, W. W.; Christensen, F. E.; Comastri, A.; Fuerst, F.; Gandhi, P.; Hailey, C. J.; Harrison, F. A.; Hickox, R. C.; Koss, M.; LaMassa, S.; Puccetti, S.; Rivers, E.; Vasudevan, R.; Walton, D. J.; Zhang, W. W.

    2015-05-01

    The covering factor of Compton-thick (CT) obscuring material associated with the torus in active galactic nuclei (AGNs) is at present best understood through the fraction of sources exhibiting CT absorption along the line of sight (NH > 1.5 × 1024 cm-2) in the X-ray band, which reveals the average covering factor. Determining this CT fraction is difficult, however, due to the extreme obscuration. With its spectral coverage at hard X-rays (>10 keV), Nuclear Spectroscopic Telescope Array (NuSTAR) is sensitive to the AGNs covering factor since Compton scattering of X-rays off optically thick material dominates at these energies. We present a spectral analysis of 10 AGNs observed with NuSTAR where the obscuring medium is optically thick to Compton scattering, so-called CT AGNs. We use the torus models of Brightman & Nandra that predict the X-ray spectrum from reprocessing in a torus and include the torus opening angle as a free parameter and aim to determine the covering factor of the CT gas in these sources individually. Across the sample we find mild to heavy CT columns, with NH measured from 1024 to 1026 cm-2, and a wide range of covering factors, where individual measurements range from 0.2 to 0.9. We find that the covering factor, fc, is a strongly decreasing function of the intrinsic 2-10 keV luminosity, LX, where fc = (-0.41 ± 0.13)log10(LX/erg s-1)+18.31 ± 5.33, across more than two orders of magnitude in LX (1041.5-1044 erg s-1). The covering factors measured here agree well with the obscured fraction as a function of LX as determined by studies of local AGNs with LX > 1042.5 erg s-1.

  9. MicroSIFT Courseware Evaluations (169-198). Set 9. Including Subject and Title Indexes Covering Sets 1-9.

    ERIC Educational Resources Information Center

    Weaver, Dave, Ed.

    This document consists of 30 microcomputer software package evaluations prepared for the MicroSIFT (Microcomputer Software and Information for Teachers) Clearinghouse at the Northwest Regional Educational Laboratory (NWREL). The concise, single-sheet resume describing and evaluating each software package includes source, cost, ability level,…

  10. Determining the Covering Factor of Compton-Thick Active Galactic Nuclei with NuSTAR

    NASA Technical Reports Server (NTRS)

    Brightman, M.; Balokovic, M.; Stern, D.; Arevalo, P.; Ballantyne, D. R.; Bauer, F. E.; Boggs, S. E.; Craig, W. W.; Christensen, F. E.; Zhang, W. W.

    2015-01-01

    The covering factor of Compton-thick (CT) obscuring material associated with the torus in active galactic nuclei (AGNs) is at present best understood through the fraction of sources exhibiting CT absorption along the line of sight (N(sub H) greater than 1.5 x 10(exp 24) cm(exp -2)) in the X-ray band, which reveals the average covering factor. Determining this CT fraction is difficult, however, due to the extreme obscuration. With its spectral coverage at hard X-rays (greater than 10 keV), Nuclear Spectroscopic Telescope Array (NuSTAR) is sensitive to the AGNs covering factor since Compton scattering of X-rays off optically thick material dominates at these energies. We present a spectral analysis of 10 AGNs observed with NuSTAR where the obscuring medium is optically thick to Compton scattering, so-called CT AGNs. We use the torus models of Brightman and Nandra that predict the X-ray spectrum from reprocessing in a torus and include the torus opening angle as a free parameter and aim to determine the covering factor of the CT gas in these sources individually. Across the sample we find mild to heavy CT columns, with N(sub H) measured from 10(exp 24) to 10(exp 26) cm(exp -2), and a wide range of covering factors, where individual measurements range from 0.2 to 0.9. We find that the covering factor, f(sub c), is a strongly decreasing function of the intrinsic 2-10 keV luminosity, L(sub X), where f(sub c) = (-0.41 +/- 0.13)log(sub 10)(L(sub X)/erg s(exp -1))+18.31 +/- 5.33, across more than two orders of magnitude in L(sub X) (10(exp 41.5) - 10(exp 44) erg s(exp -1)). The covering factors measured here agree well with the obscured fraction as a function of LX as determined by studies of local AGNs with L(sub X) greater than 10(exp 42.5) erg s(exp -1).

  11. Antiradical activity of gallic acid included in lipid interphases.

    PubMed

    Salcedo, C L; Frías, M A; Cutro, A C; Nazareno, M A; Disalvo, E A

    2014-10-01

    Polyphenols are well known as antioxidant agents and by their effects on the hydration layers of lipid interphases. Among them, gallic acid and its derivatives are able to decrease the dipole potential and to act in water as a strong antioxidant. In this work we have studied both effects on lipid interphases in monolayers and bilayers of dimyristoylphosphatidylcholine. The results show that gallic acid (GA) increases the negative surface charges of large unilamellar vesicles (LUVs) and decreases the dipole potential of the lipid interphase. As a result, positively charged radical species such as ABTS(+) are able to penetrate the membrane forming an association with GA. These results allow discussing the antiradical activity (ARA) of GA at the membrane phase which may be taking place in water spaces between the lipids.

  12. A Review of Land-Cover Mapping Activities in Coastal Alabama and Mississippi

    USGS Publications Warehouse

    Smith, Kathryn E.L.; Nayegandhi, Amar; Brock, John C.

    2010-01-01

    -based land-use classifications. Aerial photography is typically selected for smaller landscapes (watershed-basin scale), for greater definition of the land-use categories, and for increased spatial resolution. Disadvantages of using photography include time-consuming digitization, high costs for imagery collection, and lack of seasonal data. Recently, the availability of high-resolution satellite imagery has generated a new category of LULC data product. These new datasets have similar strengths to the aerial-photo-based LULC in that they possess the potential for refined definition of land-use categories and increased spatial resolution but also have the benefit of satellite-based classifications, such as repeatability for change analysis. LULC classification based on high-resolution satellite imagery is still in the early stages of development but merits greater attention because environmental-monitoring and landscape-modeling programs rely heavily on LULC data. This publication summarizes land-use and land-cover mapping activities for Alabama and Mississippi coastal areas within the U.S. Geological Survey (USGS) Northern Gulf of Mexico (NGOM) Ecosystem Change and Hazard Susceptibility Project boundaries. Existing LULC datasets will be described, as well as imagery data sources and ancillary data that may provide ground-truth or satellite training data for a forthcoming land-cover classification. Finally, potential areas for a high-resolution land-cover classification in the Alabama-Mississippi region will be identified.

  13. Including land cover change in analysis of greenness trends using all available Landsat 5, 7, and 8 images: A case study from Guangzhou, China (2000–2014)

    USGS Publications Warehouse

    Zhu, Zhe; Fu, Yingchun; Woodcock, Curtis; Olofsson, Pontus; Vogelmann, James; Holden, Christopher; Wang, Min; Dai, Shu; Yu, Yang

    2016-01-01

    An assessment of the consistency of surface reflectance from Landsat 8 with past Landsat sensors indicates biases in the visible bands of Landsat 8, especially the blue band. Landsat 8 NDVI values were found to have a larger bias than the EVI values; therefore, EVI was used in the analysis of greenness trends for Guangzhou. In spite of massive amounts of development in Guangzhou from 2000 to 2014, greenness was found to increase, mostly as a result of gradual change. Comparison of the greening magnitudes estimated from the approach presented here and a Simple Linear Trend (SLT) method indicated large differences for certain time intervals as the SLT method does not include consideration for abrupt land cover changes. Overall, this analysis demonstrates the importance of considering land cover change when analyzing trends in greenness from satellite time series in areas where land cover change is common.

  14. The Potential Radiative Forcing of Global Land Use and Land Cover Change Activities

    NASA Astrophysics Data System (ADS)

    Ward, D. S.; Mahowald, N. M.; Kloster, S.

    2014-12-01

    Given the expected increase in pressure on land resources over the next century, there is a need to understand the total impacts of activities associated with land use and land cover change (LULCC). Here we quantify these impacts using the radiative forcing metric, including forcings from changes in long-lived greenhouse gases, tropospheric ozone, aerosol effects, and land surface albedo. We estimate radiative forcings from the different agents for historical LULCC and for six future projections using simulations from the National Center for Atmospheric Research Community Land Model and Community Atmosphere Models and additional offline analyses. When all forcing agents are considered together we show that 45% (+30%, -20%) of the present-day (2010) anthropogenic radiative forcing can be attributed to LULCC. Changes in the emission of non-CO2 greenhouse gases and aerosols from LULCC enhance the total LULCC radiative forcing by a factor of 2 to 3 with respect to the forcing from CO2 alone. In contrast, the non-CO2 forcings from fossil fuel burning are roughly neutral, due largely to the negative (cooling) impact of aerosols from these sources. We partition the global LULCC radiative forcing into three major sources: direct modification of land cover (e.g. deforestation), agricultural activities, and fire regime changes. Contributions from deforestation and agriculture are roughly equal in the present day, while changes to wildfire activity impose a small negative forcing globally. In 2100, deforestation activities comprise the majority of the LULCC radiative forcing for all projections except one (Representative Concentration Pathway (RCP) 4.5). This suggests that realistic scenarios of future forest area change are essential for projecting the contribution of LULCC to climate change. However, the commonly used RCP land cover change projections all include decreases in global deforestation rates over the next 85 years. To place an upper bound on the potential

  15. A chemical kinetically based ignition delay correlation for iso-octane covering a wide range of conditions including the NTC region

    SciTech Connect

    Goldsborough, S. Scott

    2009-06-15

    An ignition delay correlation has been developed for iso-octane based on the functional behavior exhibited by a detailed chemical kinetic mechanism. The correlation employs a traditional Arrhenius-based, power law formulation, {tau}=A{phi}{sup {alpha}}p{sup {beta}}{chi}{sub O{sub 2}}{sup {gamma}} x exp ({lambda}), including dependencies for equivalence ratio ({phi}), pressure (p) and oxygen percentage ({chi}{sub O2}). However the exponents for these parameters, {alpha}, {beta}, and {gamma}, respectively, are expressed as third-order polynomials with respect to temperature in order to capture changes in functionality seen across different regimes. At very low temperatures {alpha}, {beta}, and {gamma} are forced to a constant value, as seen within the mechanism. The activation energy term, {lambda}, is written as a combination of two quadratic expressions so that the behavior in the negative temperature coefficient (NTC) region can be captured. A pressure-dependent term is also included in the expression for {lambda} in order to reduce the activation energy at higher pressures in the NTC region due to increased low temperature reactivity, and the appearance of cool flame, or low temperature heat release (LTHR). The resulting expression contains 37 constants. The new correlation is applicable over a wide range of conditions and can be used for data comparisons and mechanism evaluation, as well as systems-level engineering simulations. In this work experimental data from rapid compression machines (RCM) and shock tubes (ST) are compared through normalizing features of the correlation, and the performance of a detailed kinetic mechanism is evaluated based on the functional behavior of the {alpha}, {beta}, {gamma} and {lambda} parameters. Six hundred and sixty-one (661) data points have been used to fit the 37 constants of the expression where the experimental conditions cover {phi}=0.2-2.0, p=1-60atm, {chi}{sub O{sub 2}} and T=650-2000 K. Data normalized through the

  16. Reduction of Urease Activity by Interaction with the Flap Covering the Active Site

    PubMed Central

    Macomber, Lee; Minkara, Mona S.; Hausinger, Robert P.; Merz, Kenneth M.

    2015-01-01

    With the increasing appreciation for the human microbiome coupled with the global rise of antibiotic resistant organisms, it is imperative that new methods be developed to specifically target pathogens. To that end, a novel computational approach was devised to identify compounds that reduce the activity of urease, a medically important enzyme of Helicobacter pylori, Proteus mirabilis, and many other microorganisms. Urease contains a flexible loop that covers its active site; Glide was used to identify small molecules predicted to lock this loop in an open conformation. These compounds were screened against the model urease from Klebsiella aerogenes and the natural products epigallocatechin and quercetin were shown to inhibit at low and high micromolar concentrations, respectively. These molecules exhibit a strong time-dependent inactivation of urease that was not due to their oxygen sensitivity. Rather, these compounds appear to inactivate urease by reacting with a specific Cys residue located on the flexible loop. Substitution of this cysteine by alanine in the C319A variant increased the urease resistance to both epigallocatechin and quercetin, as predicted by the computational studies. Protein dynamics are integral to the function of many enzymes; thus, identification of compounds that lock an enzyme into a single conformation presents a useful approach to define potential inhibitors. PMID:25594724

  17. Optimal design of active and semi-active suspensions including time delays and preview

    NASA Astrophysics Data System (ADS)

    Hac', A.; Youn, I.

    1993-10-01

    Several control laws for active and semi-active suspension based on a linear half car model are derived and investigated. The strategies proposed take full advantage of the fact that the road input to the rear wheels is a delayed version of that to the front wheels, which in turn can be obtained either from the measurements of the front wheels and body motions or by direct preview of road irregularities if preview sensors are available. The suspension systems are optimized with respect to ride comfort, road holding and suspension rattle space as expressed by the mean-square-values of body acceleration (including effects of heave and pitch), tire deflections and front and rear suspension travels. The optimal control laws that minimize the given performance index and include passivity constraints in the semi-active case are derived using calculus of variation. The optimal semi-active suspension becomes piecewise linear, varying between passive and fully active systems and combinations of them. The performances of active and semi-active systems with and without preview were evaluated by numerical simulation in the time and frequency domains. The results show that incorporation of time delay between the front and rear axles in controller design improves the dynamic behavior of the rear axle and control of body pitch motion, while additional preview improves front wheel dynamics and body heave.

  18. 30 CFR 280.4 - What activities are not covered by this part?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... regulations at 30 CFR part 282; and (d) G&G exploration or G&G scientific research activities related to oil... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What activities are not covered by this part? 280.4 Section 280.4 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR...

  19. Land-cover observations as part of a Global Earth Observation System of Systems (GEOSS): progress, activities, and prospects

    USGS Publications Warehouse

    Herold, M.; Woodcock, C.E.; Loveland, Thomas R.; Townshend, J.; Brady, M.; Steenmans, C.; Schmullius, C. C.

    2008-01-01

    The international land-cover community has been working with GEO since 2005 to build the foundations for land-cover observations as an integral part of a Global Earth Observation System of Systems (GEOSS). The Group on Earth Observation (GEO) has provided the platform to elevate the societal relevance of land cover monitoring and helped to link a diverse set of global, regional, and national activities. A dedicated 2007-2009 GEO work plan task has resulted in achievements on the strategic and implementation levels. Integrated Global Observations of the Land (IGOL), the land theme of the Integrated Global Observation Strategy (IGOS), has been approved and is now in the process of transition into GEO implementation. New global land-cover maps at moderate spatial resolutions (i.e., GLOBCOVER) are being produced using guidelines and standards of the international community. The Middecadal Global Landsat Survey for 2005-2006 is extending previous 1990 and 2000 efforts for global, high-quality Landsat data. Despite this progress, essential challenges for building a sustained global land-cover-observing system remain, including: international cooperation on the continuity of global observations; ensuring consistency in land monitoring approaches; community engagement and country participation in mapping activities; commitment to ongoing quality assurance and validation; and regional networking and capacity building.

  20. 32 CFR 143.6 - Activity not covered by this part.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 1 2012-07-01 2012-07-01 false Activity not covered by this part. 143.6 Section 143.6 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN DOD POLICY ON ORGANIZATIONS THAT SEEK TO REPRESENT OR ORGANIZE MEMBERS OF THE...

  1. 29 CFR 776.3 - Persons engaging in both covered and noncovered activities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... INTERPRETATIVE BULLETIN ON THE GENERAL COVERAGE OF THE WAGE AND HOURS PROVISIONS OF THE FAIR LABOR STANDARDS ACT... activities. The Act applies to employees “engaged in commerce or in the production of goods for commerce... 29 Labor 3 2013-07-01 2013-07-01 false Persons engaging in both covered and noncovered...

  2. 29 CFR 776.3 - Persons engaging in both covered and noncovered activities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... INTERPRETATIVE BULLETIN ON THE GENERAL COVERAGE OF THE WAGE AND HOURS PROVISIONS OF THE FAIR LABOR STANDARDS ACT... activities. The Act applies to employees “engaged in commerce or in the production of goods for commerce... 29 Labor 3 2012-07-01 2012-07-01 false Persons engaging in both covered and noncovered...

  3. 29 CFR 776.3 - Persons engaging in both covered and noncovered activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... INTERPRETATIVE BULLETIN ON THE GENERAL COVERAGE OF THE WAGE AND HOURS PROVISIONS OF THE FAIR LABOR STANDARDS ACT... activities. The Act applies to employees “engaged in commerce or in the production of goods for commerce... 29 Labor 3 2011-07-01 2011-07-01 false Persons engaging in both covered and noncovered...

  4. 29 CFR 776.3 - Persons engaging in both covered and noncovered activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... INTERPRETATIVE BULLETIN ON THE GENERAL COVERAGE OF THE WAGE AND HOURS PROVISIONS OF THE FAIR LABOR STANDARDS ACT... activities. The Act applies to employees “engaged in commerce or in the production of goods for commerce... 29 Labor 3 2010-07-01 2010-07-01 false Persons engaging in both covered and noncovered...

  5. 29 CFR 776.3 - Persons engaging in both covered and noncovered activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... INTERPRETATIVE BULLETIN ON THE GENERAL COVERAGE OF THE WAGE AND HOURS PROVISIONS OF THE FAIR LABOR STANDARDS ACT... activities. The Act applies to employees “engaged in commerce or in the production of goods for commerce... 29 Labor 3 2014-07-01 2014-07-01 false Persons engaging in both covered and noncovered...

  6. 50 CFR 18.121 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... subpart cover? 18.121 Section 18.121 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... Mammals Incidental to Oil and Gas Exploration, Development, and Production Activities in the Beaufort Sea... polar bears and Pacific walruses by you (U.S. citizens as defined in § 18.27(c)) while engaged in...

  7. 50 CFR 18.121 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... subpart cover? 18.121 Section 18.121 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... Mammals Incidental to Oil and Gas Exploration, Development, and Production Activities in the Beaufort Sea... polar bears and Pacific walruses by you (U.S. citizens as defined in § 18.27(c)) while engaged in...

  8. 50 CFR 18.121 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... subpart cover? 18.121 Section 18.121 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... Mammals Incidental to Oil and Gas Exploration, Development, and Production Activities in the Beaufort Sea... polar bear and Pacific walrus by you (U.S. citizens as defined in § 18.27(c)) while engaged in oil...

  9. 50 CFR 18.121 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... subpart cover? 18.121 Section 18.121 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... Mammals Incidental to Oil and Gas Exploration, Development, and Production Activities in the Beaufort Sea... polar bears and Pacific walruses by you (U.S. citizens as defined in § 18.27(c)) while engaged in...

  10. 50 CFR 18.121 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... subpart cover? 18.121 Section 18.121 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... Mammals Incidental to Oil and Gas Exploration, Development, and Production Activities in the Beaufort Sea... polar bears and Pacific walruses by you (U.S. citizens as defined in § 18.27(c)) while engaged in...

  11. Cover crop effects on soil microbial communities and enzyme activity in semiarid agroecosystems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of this study was to compare a fallow-winter wheat (Triticum aestivum L.) rotation to several cover crop-winter wheat rotations under dryland and irrigated conditions in the semiarid US High Plains. We carried out a study that included two sites (Sidney, NE, and Akron, CO), and three s...

  12. Spreading Topsoil Encourages Ecological Restoration on Embankments: Soil Fertility, Microbial Activity and Vegetation Cover

    PubMed Central

    Rivera, Desirée; Mejías, Violeta; Jáuregui, Berta M.; López-Archilla, Ana Isabel; Peco, Begoña

    2014-01-01

    The construction of linear transport infrastructure has severe effects on ecosystem functions and properties, and the restoration of the associated roadslopes contributes to reduce its impact. This restoration is usually approached from the perspective of plant cover regeneration, ignoring plant-soil interactions and the consequences for plant growth. The addition of a 30 cm layer of topsoil is a common practice in roadslope restoration projects to increase vegetation recovery. However topsoil is a scarce resource. This study assesses the effects of topsoil spreading and its depth (10 to 30 cm) on two surrogates of microbial activity (β-glucosidase and phosphatase enzymes activity and soil respiration), and on plant cover, plant species richness and floristic composition of embankment vegetation. The study also evaluates the differences in selected physic-chemical properties related to soil fertility between topsoil and the original embankment substrate. Topsoil was found to have higher values of organic matter (11%), nitrogen (44%), assimilable phosphorous (50%) and silt content (54%) than the original embankment substrate. The topsoil spreading treatment increased microbial activity, and its application increased β-glucosidase activity (45%), phosphatase activity (57%) and soil respiration (60%). Depth seemed to affect soil respiration, β-glucosidase and phosphatase activity. Topsoil application also enhanced the species richness of restored embankments in relation to controls. Nevertheless, the depth of the spread topsoil did not significantly affect the resulting plant cover, species richness or floristic composition, suggesting that both depths could have similar effects on short-term recovery of the vegetation cover. A significant implication of these results is that it permits the application of thinner topsoil layers, with major savings in this scarce resource during the subsequent slope restoration work, but the quality of topsoil relative to the

  13. Spreading topsoil encourages ecological restoration on embankments: soil fertility, microbial activity and vegetation cover.

    PubMed

    Rivera, Desirée; Mejías, Violeta; Jáuregui, Berta M; Costa-Tenorio, Marga; López-Archilla, Ana Isabel; Peco, Begoña

    2014-01-01

    The construction of linear transport infrastructure has severe effects on ecosystem functions and properties, and the restoration of the associated roadslopes contributes to reduce its impact. This restoration is usually approached from the perspective of plant cover regeneration, ignoring plant-soil interactions and the consequences for plant growth. The addition of a 30 cm layer of topsoil is a common practice in roadslope restoration projects to increase vegetation recovery. However topsoil is a scarce resource. This study assesses the effects of topsoil spreading and its depth (10 to 30 cm) on two surrogates of microbial activity (β-glucosidase and phosphatase enzymes activity and soil respiration), and on plant cover, plant species richness and floristic composition of embankment vegetation. The study also evaluates the differences in selected physic-chemical properties related to soil fertility between topsoil and the original embankment substrate. Topsoil was found to have higher values of organic matter (11%), nitrogen (44%), assimilable phosphorous (50%) and silt content (54%) than the original embankment substrate. The topsoil spreading treatment increased microbial activity, and its application increased β-glucosidase activity (45%), phosphatase activity (57%) and soil respiration (60%). Depth seemed to affect soil respiration, β-glucosidase and phosphatase activity. Topsoil application also enhanced the species richness of restored embankments in relation to controls. Nevertheless, the depth of the spread topsoil did not significantly affect the resulting plant cover, species richness or floristic composition, suggesting that both depths could have similar effects on short-term recovery of the vegetation cover. A significant implication of these results is that it permits the application of thinner topsoil layers, with major savings in this scarce resource during the subsequent slope restoration work, but the quality of topsoil relative to the

  14. The Covering Factor of Warm Dust in Weak Emission-line Active Galactic Nuclei

    NASA Astrophysics Data System (ADS)

    Zhang, Xudong; Liu, Yuan

    2016-10-01

    Weak emission-line active galactic nuclei (WLAGNs) are radio-quiet active galactic nuclei (AGNs) that have nearly featureless optical spectra. We investigate the ultraviolet to mid-infrared spectral energy distributions of 73 WLAGNs (0.4 < z < 3) and find that most of them are similar to normal AGNs. We also calculate the covering factor of warm dust of these 73 WLAGNs. No significant difference is indicated by a KS test between the covering factor of WLAGNs and normal AGNs in the common range of bolometric luminosity. The implication for several models of WLAGNs is discussed. The super-Eddington accretion is unlikely to be the dominant reason for the featureless spectrum of a WLAGN. The present results are still consistent with the evolution scenario, i.e., WLAGNs are in a special stage of AGNs.

  15. Heritage Adoption Lessons Learned, Active Mirror Telescope Cover Deployment and Latch Mechanism

    NASA Technical Reports Server (NTRS)

    Wincentsen, James E.

    2006-01-01

    The Active Mirror Telescope (AMT) task adopted the Cover Deployment and Latch Mechanism (CDLM) design as used on the Galaxy Evolution Explorer (GALEX) project. The three separate mechanisms that comprise the CDLM will be discussed in this paper in addition to a focus on heritage adoption lessons learned and specific examples. These lessons learned will be valuable to any project considering the use of heritage designs.

  16. Study of mineral content (Nutrients and Trace elements) in vine leaf and 4 weed species included in the vegetal cover in a Spanish vineyard.

    NASA Astrophysics Data System (ADS)

    Amorós, José Angel; Bravo, Sandra; Pérez-de-los-Reyes, Caridad; Jesús García-Navarro, Francisco; Higueras, Pablo; Campos, Juan Antonio; María Moreno, Marta

    2016-04-01

    The content of some mineral elements (Na, Ca, Mg, K, P, S, Fe, Mn, Si, Al, V, Cr, Cu, Rb, Sr, Ba, Zn, Pb, Ce, La and Nd) has been studied in vine leaf and four weed species (Mendicago lupulina L.; Malva sylvestris L., Hordeum murinum L. and Scandix pecten-veneris L.) included in the natural vegetal cover of a vineyard sited in Ciudad Real province (Central Spain). Samples were taken in May 2015, dried and milled in order to analyze them using the X Ray Fluorescence Technique. The results obtained have been compared with those measured in a vineyard located in a different site and with those suggested by the literature consulted for plants in general all around the world. The results indicate that some differences in mineral content among the weed species can be drafted. Great differences have been found in K, Si, Ca and Zn, although other elements, such as Mg, P, S, Ba and Nd, remained almost constant despite of the species. Moreover, the influence of the type of soil (different site) can give a different composition of the vine leaf in some elements. This last point is especially evident in the case of the Sr (more present in calcareous soils and leaves of plants grown on them, reaching 377 mg kg-1 versus less than 86 mg kg-1 in the non-calcareous studied soil).

  17. Evaluation of a biologically active cover for mitigation of landfill gas emissions.

    PubMed

    Barlaz, M A; Green, R B; Chanton, J P; Goldsmith, C D; Hater, G R

    2004-09-15

    Landfills are the third largest source of anthropogenic CH4 in the United States, and there is potential for reduction in this source of greenhouse gases and other contaminants. The objective of this work was to contrast emissions of CH4 and non-methane organic compounds (NMOCs) from landfill cells covered with soil or a biologically active cover consisting of yard waste compost. On the basis of four field campaigns over 14 months, CH4 emissions from the biocover (BC) varied from -1.73 to 1.33 g m(-2) d(-1), with atmospheric uptake measured in 52% of tests. BC emissions did not increase when the gas collection system was turned off. Uptake of atmospheric CH4 was measured in 54% of tests on the soil cover (SC) when the gas collection was system active and 12% when the gas collection system was off. Many (26%) relatively high fluxes (>15 g m(-2) d(-1)) were measured from the SC as were some dramatic effects due to deactivation of the gas collection system. In tests with positive emissions, stable isotope measurements showed that the BC and SC were responsible for oxidation of 55% and 21% of the CH4 reaching the bottom of the respective cover. Seven of the highest 10 NMOC emissions were measured in the SC, and 17 of 21 fluxes for speciated organic compounds were higher in the SC. The relationship between CH4, NMOC, and individual organic compound emissions suggested a correlation between CH4 and trace organic oxidation. BCs can reduce landfill gas emissions in the absence of a gas collection system and can serve as a polishing step in the presence of an active system.

  18. 45 CFR 90.3 - What programs or activities does the Age Discrimination Act of 1975 cover?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Discrimination Act of 1975 cover? 90.3 Section 90.3 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES... FINANCIAL ASSISTANCE General § 90.3 What programs or activities does the Age Discrimination Act of 1975 cover? (a) The Age Discrimination Act of 1975 applies to any program or activity receiving...

  19. Above- and below-ground methane fluxes and methanotrophic activity in a landfill-cover soil

    SciTech Connect

    Schroth, M.H.; Eugster, W.; Gomez, K.E.; Gonzalez-Gil, G.; Niklaus, P.A.; Oester, P.

    2012-05-15

    Highlights: Black-Right-Pointing-Pointer We quantify above- and below-ground CH{sub 4} fluxes in a landfill-cover soil. Black-Right-Pointing-Pointer We link methanotrophic activity to estimates of CH{sub 4} loading from the waste body. Black-Right-Pointing-Pointer Methane loading and emissions are highly variable in space and time. Black-Right-Pointing-Pointer Eddy covariance measurements yield largest estimates of CH{sub 4} emissions. Black-Right-Pointing-Pointer Potential methanotrophic activity is high at a location with substantial CH{sub 4} loading. - Abstract: Landfills are a major anthropogenic source of the greenhouse gas methane (CH{sub 4}). However, much of the CH{sub 4} produced during the anaerobic degradation of organic waste is consumed by methanotrophic microorganisms during passage through the landfill-cover soil. On a section of a closed landfill near Liestal, Switzerland, we performed experiments to compare CH{sub 4} fluxes obtained by different methods at or above the cover-soil surface with below-ground fluxes, and to link methanotrophic activity to estimates of CH{sub 4} ingress (loading) from the waste body at selected locations. Fluxes of CH{sub 4} into or out of the cover soil were quantified by eddy-covariance and static flux-chamber measurements. In addition, CH{sub 4} concentrations at the soil surface were monitored using a field-portable FID detector. Near-surface CH{sub 4} fluxes and CH{sub 4} loading were estimated from soil-gas concentration profiles in conjunction with radon measurements, and gas push-pull tests (GPPTs) were performed to quantify rates of microbial CH{sub 4} oxidation. Eddy-covariance measurements yielded by far the largest and probably most representative estimates of overall CH{sub 4} emissions from the test section (daily mean up to {approx}91,500 {mu}mol m{sup -2} d{sup -1}), whereas flux-chamber measurements and CH{sub 4} concentration profiles indicated that at the majority of locations the cover soil was a

  20. Friction-induced surface activity of some hydrocarbons with clean and oxide-covered iron

    NASA Technical Reports Server (NTRS)

    Buckley, D. H.

    1973-01-01

    Sliding friction studies were conducted on a clean and oxide-covered iron surface with exposure of that surface to various hydrocarbons. The hydrocarbons included ethane, ethylene ethyl chloride, methyl chloride, and vinyl chloride. Auger cylindrical mirror analysis was used to follow interactions of the hydrocarbon with the iron surface. Results with vinyl chloride indicate friction induced surface reactivity, adsorption to surface oxides, friction sensitivity to concentration and polymerization. Variation in the loads employed influence adsorption and accordingly friction. In contrast with ethyl and vinyl chloride, friction induced surface reactivity was not observed with ethane and ethylene.

  1. Improvements to the FATOLA computer program including added actively controlled landing gear subroutines

    NASA Technical Reports Server (NTRS)

    Mall, G. H.

    1983-01-01

    Modifications to a multi-degree-of-freedom flexible aircraft take-off and landing analysis (FATOLA) computer program, including a provision for actively controlled landing gears to expand the programs simulation capabilities, are presented. Supplemental instructions for preparation of data and for use of the modified program are included.

  2. Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake.

    PubMed

    Bižić-Ionescu, Mina; Amann, Rudolf; Grossart, Hans-Peter

    2014-01-01

    In winter 2009/10, a sudden under-ice bloom of heterotrophic bacteria occurred in the seasonally ice-covered, temperate, deep, oligotrophic Lake Stechlin (Germany). Extraordinarily high bacterial abundance and biomass were fueled by the breakdown of a massive bloom of Aphanizomenon flos-aquae after ice formation. A reduction in light resulting from snow coverage exerted a pronounced physiological stress on the cyanobacteria. Consequently, these were rapidly colonized, leading to a sudden proliferation of attached and subsequently of free-living heterotrophic bacteria. Total bacterial protein production reached 201 µg C L(-1) d(-1), ca. five times higher than spring-peak values that year. Fluorescence in situ hybridization and denaturing gradient gel electrophoresis at high temporal resolution showed pronounced changes in bacterial community structure coinciding with changes in the physiology of the cyanobacteria. Pyrosequencing of 16S rRNA genes revealed that during breakdown of the cyanobacterial population, the diversity of attached and free-living bacterial communities were reduced to a few dominant families. Some of these were not detectable during the early stages of the cyanobacterial bloom indicating that only specific, well adapted bacterial communities can colonize senescent cyanobacteria. Our study suggests that in winter, unlike commonly postulated, carbon rather than temperature is the limiting factor for bacterial growth. Frequent phytoplankton blooms in ice-covered systems highlight the need for year-round studies of aquatic ecosystems including the winter season to correctly understand element and energy cycling through aquatic food webs, particularly the microbial loop. On a global scale, such knowledge is required to determine climate change induced alterations in carbon budgets in polar and temperate aquatic systems.

  3. Active thermography and post-processing image enhancement for recovering of abraded and paint-covered alphanumeric identification marks

    NASA Astrophysics Data System (ADS)

    Montanini, R.; Quattrocchi, A.; Piccolo, S. A.

    2016-09-01

    Alphanumeric marking is a common technique employed in industrial applications for identification of products. However, the realised mark can undergo deterioration, either by extensive use or voluntary deletion (e.g. removal of identification numbers of weapons or vehicles). For recovery of the lost data many destructive or non-destructive techniques have been endeavoured so far, which however present several restrictions. In this paper, active infrared thermography has been exploited for the first time in order to assess its effectiveness in restoring paint covered and abraded labels made by means of different manufacturing processes (laser, dot peen, impact, cold press and scribe). Optical excitation of the target surface has been achieved using pulse (PT), lock-in (LT) and step heating (SHT) thermography. Raw infrared images were analysed with a dedicated image processing software originally developed in Matlab™, exploiting several methods, which include thermographic signal reconstruction (TSR), guided filtering (GF), block guided filtering (BGF) and logarithmic transformation (LN). Proper image processing of the raw infrared images resulted in superior contrast and enhanced readability. In particular, for deeply abraded marks, good outcomes have been obtained by application of logarithmic transformation to raw PT images and block guided filtering to raw phase LT images. With PT and LT it was relatively easy to recover labels covered by paint, with the latter one providing better thermal contrast for all the examined targets. Step heating thermography never led to adequate label identification instead.

  4. Population and Human Development: A Course Curriculum Including Lesson Plans, Activities, and Bibliography. Revised.

    ERIC Educational Resources Information Center

    Murphy, Elaine M.; Long, Alison T.

    This course outline suggests materials and learning activities on the interrelated causes and consequences of population growth and other population matters. The document describes 15 class sessions which integrate information for sociology, anthropology, psychology, biology, animal behavior, and education. Topics include the history of human…

  5. 76 FR 10385 - Agency Information Collection Activities: Various Contract Related Forms That Will be Included in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... SECURITY Agency Information Collection Activities: Various Contract Related Forms That Will be Included in the Homeland Security Acquisition Regulation, DHS FORM 0700-01, DHS FORM 0700-02, DHS FORM 0700-03...: The Department of Homeland Security, Office of Chief Procurement Officer, Acquisition Policy...

  6. Field-Scale Stable-Isotope Probing of Active Methanotrophs in a Landfill-Cover Soil

    NASA Astrophysics Data System (ADS)

    Schroth, M. H.; Henneberger, R.; Chiri, E.

    2012-12-01

    The greenhouse gas methane (CH4) is an important contributor to global climate change. While its atmospheric concentration is increasing, a large portion of produced CH4 never reaches the atmosphere, but is consumed by aerobic methane-oxidizing bacteria (MOB). The latter are ubiquitous in soils and utilize CH4 as sole source of energy and carbon. Among other methods, MOB may be differentiated based on characteristic phospholipid fatty acids (PLFA). Stable-isotope probing (SIP) on PLFA has been widely applied to identify active members of MOB communities in laboratory incubation studies, but results are often difficult to extrapolate to the field. Thus, novel field-scale approaches are needed to link activity and identity of MOB in their natural environment. We present results of field experiments in which we combined PLFA-SIP with gas push-pull tests (GPPTs) to label active MOB at the field-scale while simultaneously quantifying CH4 oxidation activity. During a SIP-GPPT, a mixture of reactive (here 13CH4, O2) and non-reactive tracer gases (e.g., Ar, Ne, He) is injected into the soil at a location of interest. Thereafter, gas flow is reversed and the gas mixture diluted with soil air is extracted from the same location and sampled periodically. Rate constants for CH4 oxidation can be calculated by analyzing breakthrough curves of 13CH4 and a suitable non-reactive tracer gas. SIP-GPPTs were performed in a landfill-cover soil, and feasibility of this novel approach was tested at several locations along a gradient of MOB activity and soil temperature. Soil samples were collected before and after SIP-GPPTs, total PLFA were extracted, and incorporation of 13C in the polar lipid fraction was analyzed. Potential CH4 oxidation rates derived from SIP-GPPTs were similar to those derived from regular GPPTs (using unlabeled CH4) performed at the same locations prior to SIP-GPPTs, indicating that application of 13CH4 did not adversely affect bacterial CH4 oxidation rates. Rates

  7. Optimization and validation of a quantitative liquid chromatography-tandem mass spectrometric method covering 295 bacterial and fungal metabolites including all regulated mycotoxins in four model food matrices.

    PubMed

    Malachová, Alexandra; Sulyok, Michael; Beltrán, Eduardo; Berthiller, Franz; Krska, Rudolf

    2014-10-01

    An LC-MS/MS "dilute and shoot" method for the determination of 295 fungal and bacterial metabolites was optimized and validated according to the guidelines established in the Directorate General for Health and Consumer Affairs of the European Commission (SANCO) document No. 12495/2011. Four different types of food matrices were chosen for validation: apple puree for infants (high water content), hazelnuts (high fat content), maize (high starch and low fat content) and green pepper (difficult or unique matrix). Method accuracy and precision was evaluated using spiked samples in five replicates at two concentration levels. Method trueness was demonstrated through participation in various proficiency tests. Although the method covers a total number of 331 analytes, validation data were acquired only for 295 analytes, either due to the non-availability of analytical standards or due other reasons described in this paper. Concerning the apparent recovery, the percentage of 295 analytes matching the acceptable recovery range of 70-120% lied down by SANCO varied from 21% in green pepper to 74% in apple puree at the highest spiking level. At the levels close to limit of quantification only 20-58% of the analytes fulfilled this criterion. The extent of matrix effects was strongly dependent on the analyte/matrix combination. In general, the lowest matrix effects were observed in apple puree (59% of analytes were not influenced by enhancement/suppression at all at the highest validation level). The highest matrix effects were observed in green pepper, where only 10% of analytes did not suffer from signal suppression/enhancement. The repeatability of the method was acceptable (RSD≤20) for 97% of all analytes in apple puree and hazelnuts, for 95% in maize and for 89% in green pepper. Concerning the trueness of the method, Z-scores were generally between -2 and 2, despite a broad variety of different matrices. Based on these results it can be concluded that quantitative

  8. Completed Research in Health, Physical Education, Recreation and Dance; Including International Sources. Volume 23. 1981 Edition. Covering Research Completed in 1980.

    ERIC Educational Resources Information Center

    Rothstein, Anne L., Ed.; Nelson, Jack, Ed.

    Citations of published research reports and abstracts of master's and doctoral theses completed during 1980 are included in the areas of health, physical education, recreation, dance, and allied fields in two sections of this three-section volume. The bibliography section contains listings of 1031 articles published in 113 periodicals. In a…

  9. The VIMOS VLT Deep Survey: Final Public Release of ~ 35 000 Galaxies and Active Galactic Nuclei Covering 13 Billion Years of Evolution

    NASA Astrophysics Data System (ADS)

    Le Fèvre, O.; Adami, C.; Arnouts, S.; Bardelli, S.; Bolzonella, M.; Bondi, M.; Bongiorno, A.; Bottini, D.; Cappi, A.; Cassata, P.; Charlot, S.; Ciliegi, P.; Contini, T.; Cucciati, O.; de la Torre, S.; Foucaud, S.; Franzetti, P.; Garilli, B.; Gavignaud, I.; Guzzo, L.; Ilbert, O.; Iovino, A.; Le Brun, V.; Lemaux, B.; López-Sanjuan, C.; Maccagni, D.; McCracken, H. J.; Marano, B.; Marinoni, C.; Mazure, A.; Mellier, Y.; Merighi, R.; Merluzzi, P.; Moreau, C.; Paltani, S.; Pellò, R.; Pollo, A.; Pozzetti, L.; Scaramella, R.; Scodeggio, M.; Tasca, L.; Tresse, L.; Vergani, D.; Vettolani, G.; Zamorani, G.; Zanichelli, A.; Zucca, E.

    2014-03-01

    The VIMOS VLT Deep Survey (VVDS) final and public data release offers an excellent opportunity to revisit galaxy evolution with a sample of 35 016 galaxies and active galactic nuclei covering the redshift range 0 < z < 6.7. The VVDS includes three tiered surveys, the wide, deep and ultra-deep surveys, covering up to 8.7 square degrees, and each magnitude-selected with limits iAB = 22.5, 24 and 24.75 respectively. The VVDS redshifts, spectra, and all associated multi-wavelength data are available at http://cesam.lam.fr/vvds. The highlights and scientific legacy of the VVDS are summarised.

  10. Cover crops increase foraging activity of omnivorous predators in seed patches and facilitate weed biological control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Omnivores are important consumers of both weed seeds and insect pests, and habitat provisions like cover crops are suggested to promote their ecosystem services in agricultural systems. However, few studies establish direct links between cover, food, and pest suppression because they are entangled a...

  11. Increased plastic litter cover affects the foraging activity of the sandy intertidal gastropod Nassarius pullus.

    PubMed

    Aloy, Alexander B; Vallejo, Benjamin M; Juinio-Meñez, Marie Antonette

    2011-08-01

    This study analyzed the foraging behavior of the gastropod Nassarius pullus on garbage-impacted sandy shores of Talim Bay, Batangas, Philippines. The effect of different levels of plastic garbage cover on foraging efficiency was investigated. Controlled in situ baiting experiments were conducted to quantify aspects of foraging behavior as affected by the levels of plastic litter cover in the foraging area. The results of the study indicated that the gastropod's efficiency in locating and in moving towards a food item generally decreased as the level of plastic cover increased. Prolonged food searching time and increased self-burial in sand were highly correlated with increased plastic cover. The accuracy of orientation towards the actual position of the bait decreased significantly when the amount of plastic cover increased to 50%. These results are consistent with the significant decreases in the abundance of the gastropod observed during periods of deposition of large amounts of plastic and other debris on the shore.

  12. Sky cover

    NASA Astrophysics Data System (ADS)

    Gerth, Jordan J.

    Of all of the standard meteorological parameters collected and observed daily, sky cover is not only one of the most complex, but the one that is fairly ambiguously defined and difficult to quantify. Despite that, the implications of how cloud fraction and sky cover are understood not only impact daily weather forecasts, but also present challenges to assessing the state of the earth's climate system. Part of the reason for this is the lack of observational methods for verifying the skill of clouds represented and parameterized in numerical models. While human observers record sky cover as part of routine duties, the spatial coverage of such observations in the United States is relatively sparse. There is greater spatial coverage of automated observations, and essentially complete coverage from geostationary weather satellites that observe the Americas. A good analysis of sky cover reconciles differences between manual observations, automated observations, and satellite observations, through an algorithm that accounts for the strengths and weaknesses of each dataset. This work describes the decision structure for trusting and weighting these similar observations. Some of the issues addressed include: human and instrument error resulting from approximations and estimations, a deficiency in high cloud detectability using surface-based ceilometers, poorly resolved low cloud using infrared channels on space-based radiometers during overnight hours, and decreased confidence in satellite-detected cloud during stray light periods. Using the blended sky cover analysis as the best representation of cloudiness, it is possible to compare the analysis to numerical model fields in order to assess the performance of the model and the parameterizations therein, as well as confirm or uncover additional relationships between sky cover and pertinent fields using an optimization methodology. The optimizer minimizes an affine expression of adjusted fields to the "truth" sky cover

  13. Hydrological response to land cover changes and human activities in arid regions using a geographic information system and remote sensing.

    PubMed

    Mahmoud, Shereif H; Alazba, A A

    2015-01-01

    The hydrological response to land cover changes induced by human activities in arid regions has attracted increased research interest in recent decades. The study reported herein assessed the spatial and quantitative changes in surface runoff resulting from land cover change in the Al-Baha region of Saudi Arabia between 1990 and 2000 using an ArcGIS-surface runoff model and predicted land cover and surface runoff depth in 2030 using Markov chain analysis. Land cover maps for 1990 and 2000 were derived from satellite images using ArcGIS 10.1. The findings reveal a 26% decrease in forest and shrubland area, 28% increase in irrigated cropland, 1.5% increase in sparsely vegetated land and 0.5% increase in bare soil between 1990 and 2000. Overall, land cover changes resulted in a significant decrease in runoff depth values in most of the region. The decrease in surface runoff depth ranged from 25-106 mm/year in a 7020-km2 area, whereas the increase in such depth reached only 10 mm/year in a 243-km2 area. A maximum increase of 73 mm/year was seen in a limited area. The surface runoff depth decreased to the greatest extent in the central region of the study area due to the huge transition in land cover classes associated with the construction of 25 rainwater harvesting dams. The land cover prediction revealed a greater than twofold increase in irrigated cropland during the 2000-2030 period, whereas forest and shrubland are anticipated to occupy just 225 km2 of land area by 2030, a significant decrease from the 747 km2 they occupied in 2000. Overall, changes in land cover are predicted to result in an annual increase in irrigated cropland and dramatic decline in forest area in the study area over the next few decades. The increase in surface runoff depth is likely to have significant implications for irrigation activities.

  14. Hydrological Response to Land Cover Changes and Human Activities in Arid Regions Using a Geographic Information System and Remote Sensing

    PubMed Central

    Mahmoud, Shereif H.; Alazba, A. A.

    2015-01-01

    The hydrological response to land cover changes induced by human activities in arid regions has attracted increased research interest in recent decades. The study reported herein assessed the spatial and quantitative changes in surface runoff resulting from land cover change in the Al-Baha region of Saudi Arabia between 1990 and 2000 using an ArcGIS-surface runoff model and predicted land cover and surface runoff depth in 2030 using Markov chain analysis. Land cover maps for 1990 and 2000 were derived from satellite images using ArcGIS 10.1. The findings reveal a 26% decrease in forest and shrubland area, 28% increase in irrigated cropland, 1.5% increase in sparsely vegetated land and 0.5% increase in bare soil between 1990 and 2000. Overall, land cover changes resulted in a significant decrease in runoff depth values in most of the region. The decrease in surface runoff depth ranged from 25-106 mm/year in a 7020-km2 area, whereas the increase in such depth reached only 10 mm/year in a 243-km2 area. A maximum increase of 73 mm/year was seen in a limited area. The surface runoff depth decreased to the greatest extent in the central region of the study area due to the huge transition in land cover classes associated with the construction of 25 rainwater harvesting dams. The land cover prediction revealed a greater than twofold increase in irrigated cropland during the 2000-2030 period, whereas forest and shrubland are anticipated to occupy just 225 km2 of land area by 2030, a significant decrease from the 747 km2 they occupied in 2000. Overall, changes in land cover are predicted to result in an annual increase in irrigated cropland and dramatic decline in forest area in the study area over the next few decades. The increase in surface runoff depth is likely to have significant implications for irrigation activities. PMID:25923712

  15. Active seat suspension for a small vehicle: considerations for control system including observer

    NASA Astrophysics Data System (ADS)

    Katsumata, Hiroyuki; Shiino, Hiroshi; Oshinoya, Yasuo; Ishibashi, Kazuhisa; Ozaki, Koichi; Ogino, Hirohiko

    2007-12-01

    We have examined the improvement of ride quality and the reduction of riding fatigue brought about by the active control of the seat suspension of small vehicles such as one-seater electric automobiles. A small active seat suspension, which is easy to install, was designed and manufactured for one-seater electric automobiles. For the actuator, a maintenance-free voice coil motor used as a direct drive was adopted. For fundamental considerations, we designed a one-degree-of-freedom model for the active seat suspension system. Then, we designed a disturbance cancellation control system that includes the observer for a two-degree-of-freedom model. In an actual driving test, a test road, in which the concavity and convexity of an actual road surface were simulated using hard rubber, was prepared and the control performance of vertical vibrations of the seat surface during driving was examined. As a result, in comparison with the one-degree-of-freedom control system, it was confirmed that the control performance was improved by the two-degree-of-freedom control system that includes the observer.

  16. Enzymatically active 2',5'-oligoadenylate synthetases are widely distributed among Metazoa, including protostome lineage.

    PubMed

    Päri, Mailis; Kuusksalu, Anne; Lopp, Annika; Kjaer, Karina Hansen; Justesen, Just; Kelve, Merike

    2014-02-01

    2',5'-Oligoadenylate synthetases (OASs) belong to the nucleotidyl transferase family together with poly(A) polymerases, CCA-adding enzymes and the recently discovered cyclic-GMP-AMP synthase (cGAS). Mammalian OASs have been thoroughly characterized as components of the interferon-induced antiviral system. The OAS activity and the respective genes were also discovered in marine sponges where the interferon system is absent. In this study the recombinant OASs from several multicellular animals and their closest unicellular relative, a choanoflagellate, were expressed in a bacterial expression system and their enzymatic activities were examined. We demonstrated 2-5A synthesizing activities of OASs from the marine sponge Tedania ignis, a representative of the phylogenetically oldest metazoan phylum (Porifera), from an invertebrate of the protostome lineage, the mollusk Mytilus californianus (Mollusca), and from a vertebrate species, a cartilaginous fish Leucoraja erinacea (Chordata). However, the expressed proteins from an amphibian, the salamander Ambystoma mexicanum (Chordata), and from a protozoan, the marine choanoflagellate Monosiga brevicollis (Choanozoa), did not show 2-5A synthesizing activity. Differently from other studied OASs, OAS from the marine sponge T. ignis was able to catalyze the formation of oligomers having both 2',5'- and 3',5'-phosphodiester linkages. Our data suggest that OASs from sponges and evolutionarily higher animals have similar activation mechanisms which still include different affinities and possibly different structural requirements for the activating RNAs. Considering their 2'- and 3'-specificities, sponge OASs could represent a link between evolutionarily earlier nucleotidyl transferases and 2'-specific OASs from higher animals.

  17. Enzymatically active 2',5'-oligoadenylate synthetases are widely distributed among Metazoa, including protostome lineage.

    PubMed

    Päri, Mailis; Kuusksalu, Anne; Lopp, Annika; Kjaer, Karina Hansen; Justesen, Just; Kelve, Merike

    2014-02-01

    2',5'-Oligoadenylate synthetases (OASs) belong to the nucleotidyl transferase family together with poly(A) polymerases, CCA-adding enzymes and the recently discovered cyclic-GMP-AMP synthase (cGAS). Mammalian OASs have been thoroughly characterized as components of the interferon-induced antiviral system. The OAS activity and the respective genes were also discovered in marine sponges where the interferon system is absent. In this study the recombinant OASs from several multicellular animals and their closest unicellular relative, a choanoflagellate, were expressed in a bacterial expression system and their enzymatic activities were examined. We demonstrated 2-5A synthesizing activities of OASs from the marine sponge Tedania ignis, a representative of the phylogenetically oldest metazoan phylum (Porifera), from an invertebrate of the protostome lineage, the mollusk Mytilus californianus (Mollusca), and from a vertebrate species, a cartilaginous fish Leucoraja erinacea (Chordata). However, the expressed proteins from an amphibian, the salamander Ambystoma mexicanum (Chordata), and from a protozoan, the marine choanoflagellate Monosiga brevicollis (Choanozoa), did not show 2-5A synthesizing activity. Differently from other studied OASs, OAS from the marine sponge T. ignis was able to catalyze the formation of oligomers having both 2',5'- and 3',5'-phosphodiester linkages. Our data suggest that OASs from sponges and evolutionarily higher animals have similar activation mechanisms which still include different affinities and possibly different structural requirements for the activating RNAs. Considering their 2'- and 3'-specificities, sponge OASs could represent a link between evolutionarily earlier nucleotidyl transferases and 2'-specific OASs from higher animals. PMID:24184688

  18. Soil Moisture and Snow Cover: Active or Passive Elements of Climate

    NASA Technical Reports Server (NTRS)

    Oglesby, Robert J.; Marshall, Susan; Erickson, David J., III; Robertson, Franklin R.; Roads, John O.; Arnold, James E. (Technical Monitor)

    2002-01-01

    A key question is the extent to which surface effects such as soil moisture and snow cover are simply passive elements or whether they can affect the evolution of climate on seasonal and longer time scales. We have constructed ensembles of predictability studies using the NCAR CCM3 in which we compared the relative roles of initial surface and atmospheric conditions over the central and western U.S. in determining the subsequent evolution of soil moisture and of snow cover. Results from simulations with realistic soil moisture anomalies indicate that internal climate variability may be the strongest factor, with some indication that the initial atmospheric state is also important. Model runs with exaggerated soil moisture reductions (near-desert conditions) showed a much larger effect, with warmer surface temperatures, reduced precipitation, and lower surface pressures; the latter indicating a response of the atmospheric circulation. These results suggest the possibility of a threshold effect in soil moisture, whereby an anomaly must be of a sufficient size before it can have a significant impact on the atmospheric circulation and climate. Results from simulations with realistic snow cover anomalies indicate that the time of year can be crucial. When introduced in late winter, these anomalies strongly affected the subsequent evolution of snow cover. When introduced in early winter, however, little or no effect is seen on the subsequent snow cover. Runs with greatly exaggerated initial snow cover indicate that the high reflectivity of snow is the most important process by which snow cover can impact climate, through lower surface temperatures and increased surface pressures. The results to date were obtained for model runs with present-day conditions. We are currently analyzing runs made with projected forcings for the 21st century to see if these results are modified in any way under likely scenarios of future climate change. An intriguing new statistical technique

  19. Plants as sources of airborne bacteria, including ice nucleation-active bacteria.

    PubMed

    Lindemann, J; Constantinidou, H A; Barchet, W R; Upper, C D

    1982-11-01

    Vertical wind shear and concentration gradients of viable, airborne bacteria were used to calculate the upward flux of viable cells above bare soil and canopies of several crops. Concentrations at soil or canopy height varied from 46 colony-forming units per m over young corn and wet soil to 663 colony-forming units per m over dry soil and 6,500 colony-forming units per m over a closed wheat canopy. In simultaneous samples, concentrations of viable bacteria in the air 10 m inside an alfalfa field were fourfold higher than those over a field with dry, bare soil immediately upwind. The upward flux of viable bacteria over alfalfa was three- to fourfold greater than over dry soil. Concentrations of ice nucleation-active bacteria were higher over plants than over soil. Thus, plant canopies may constitute a major source of bacteria, including ice nucleation-active bacteria, in the air.

  20. Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9.

    PubMed

    Koo, Taeyoung; Lee, Jungjoon; Kim, Jin-Soo

    2015-06-01

    Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system are now widely used for genome editing in higher eukaryotic cells and whole organisms, revolutionising almost every discipline in biological research, medicine, and biotechnology. All of these nucleases, however, induce off-target mutations at sites homologous in sequence with on-target sites, limiting their utility in many applications including gene or cell therapy. In this review, we compare methods for detecting nuclease off-target mutations. We also review methods for profiling genome-wide off-target effects and discuss how to reduce or avoid off-target mutations.

  1. Stability and activity of alcohol dehydrogenases in W/O-microemulsions: enantioselective reduction including cofactor regeneration.

    PubMed

    Orlich, B; Berger, H; Lade, M; Schomäcker, R

    2000-12-20

    Microemulsions provide an interesting alternative to classical methods for the conversion of less water-soluble substrates by alcohol dehydrogenase, but until now stability and activity were too low for economically useful processes. The activity and stability of the enzymes are dependent on the microemulsion composition, mostly the water and the surfactant concentration. Therefore, it is necessary to know the exact phase behavior of a given microemulsion reaction system and the corresponding enzyme behavior therein. Because of their economic and ecologic suitability polyethoxylated fatty alcohols were investigated concerning their phase behavior and their compatibility with enzymes in ternary mixtures. The phase behavior of Marlipal O13-60 (C13EO6 in industrial quality)/cyclohexane/water and its effect on the activity and stability of alcohol dehydrogenase from Yeast (YADH) and horse liver (HLADH) and the carbonyl reductase from Candida parapsilosis (CPCR) is presented in this study. Beside the macroscopic phase behavior of the reaction system, the viscosity of the system indicates structural changes of aggregates in the microemulsion. The changes of the enzyme activities with the composition are discussed on the basis of transitions from reverse micelles to swollen reverse micelles and finally, the transition to the phase separation. The formate dehydrogenase from Candida boidinii was used for the NADH-regeneration during reduction reactions. While the formate dehydrogenase did not show any kinetic effect on the microemulsion composition, the other enzymes show significant changes of activity and stability varying the water or surfactant concentration of the microemulsion. Under certain conditions, stability could be maintained with HLADH for several weeks. Successful experiments with semi-batch processes including cofactor regeneration and product separation were performed.

  2. Does grazing of cover crops impact biologically active soil C and N fractions under inversion and no tillage management?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cover crops are a key component of conservation cropping systems. They can also be a key component of integrated crop-livestock systems by offering high-quality forage during short periods between cash crops. The impact of cattle grazing on biologically active soil C and N fractions has not receiv...

  3. USDA Human Nutrition Research and Education Activities. A Report to Congress Covering the Period January-December 1992.

    ERIC Educational Resources Information Center

    Dupont, Jacqueline; And Others

    This document is the sixth annual, legislatively mandated report on the human nutrition research and education activities of the United States Department of Agriculture for fiscal year 1992 in which directions and highlights are emphasized. The report contains six sections. Section 1 is an introduction. Section 2 covers human nutrition research…

  4. 18 CFR 1309.4 - What programs or activities are covered by the Act and this part?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true What programs or activities are covered by the Act and this part? 1309.4 Section 1309.4 Conservation of Power and Water... purpose legislative body which: (i) Provides any benefits or assistance to persons based on age; or...

  5. 18 CFR 1309.4 - What programs or activities are covered by the Act and this part?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false What programs or activities are covered by the Act and this part? 1309.4 Section 1309.4 Conservation of Power and Water... purpose legislative body which: (i) Provides any benefits or assistance to persons based on age; or...

  6. 18 CFR 1309.4 - What programs or activities are covered by the Act and this part?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false What programs or activities are covered by the Act and this part? 1309.4 Section 1309.4 Conservation of Power and Water... purpose legislative body which: (i) Provides any benefits or assistance to persons based on age; or...

  7. 18 CFR 1309.4 - What programs or activities are covered by the Act and this part?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false What programs or activities are covered by the Act and this part? 1309.4 Section 1309.4 Conservation of Power and Water... purpose legislative body which: (i) Provides any benefits or assistance to persons based on age; or...

  8. 18 CFR 1309.4 - What programs or activities are covered by the Act and this part?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 2 2012-04-01 2012-04-01 false What programs or activities are covered by the Act and this part? 1309.4 Section 1309.4 Conservation of Power and Water... purpose legislative body which: (i) Provides any benefits or assistance to persons based on age; or...

  9. Soil Moisture and Snow Cover: Active or Passive Elements of Climate?

    NASA Technical Reports Server (NTRS)

    Oglesby, Robert J.; Marshall, Susan; Erickson, David J., III; Robertson, Franklin R.; Roads, John O.; Arnold, James E. (Technical Monitor)

    2002-01-01

    A key question in the study of the hydrologic cycle is the extent to which surface effects such as soil moisture and snow cover are simply passive elements or whether they can affect the evolution of climate on seasonal and longer time scales. We have constructed ensembles of predictability studies using the NCAR CCM3 in which we compared the relative roles of initial surface and atmospheric conditions over the central and western U.S. in determining the subsequent evolution of soil moisture and of snow cover. We have also made sensitivity studies with exaggerated soil moisture and snow cover anomalies in order to determine the physical processes that may be important. Results from simulations with realistic soil moisture anomalies indicate that internal climate variability may be the strongest factor, with some indication that the initial atmospheric state is also important. The initial state of soil moisture does not appear important, a result that held whether simulations were started in late winter or late spring. Model runs with exaggerated soil moisture reductions (near-desert conditions) showed a much larger effect, with warmer surface temperatures, reduced precipitation, and lower surface pressures; the latter indicating a response of the atmospheric circulation. These results suggest the possibility of a threshold effect in soil moisture, whereby an anomaly must be of a sufficient size before it can have a significant impact on the atmospheric circulation and hence climate. Results from simulations with realistic snow cover anomalies indicate that the time of year can be crucial. When introduced in late winter, these anomalies strongly affected the subsequent evolution of snow cover. When introduced in early winter, however, little or no effect is seen on the subsequent snow cover. Runs with greatly exaggerated initial snow cover indicate that the high reflectively of snow is the most important process by which snow cover cart impact climate, through lower

  10. Soil Moisture and Snow Cover: Active or Passive Elements of Climate?

    NASA Technical Reports Server (NTRS)

    Oglesby, Robert J.; Marshall, Susan; Robertson, Franklin R.; Roads, John O.; Arnold, James E. (Technical Monitor)

    2001-01-01

    A key question in the study of the hydrologic cycle is the extent to which surface effects such as soil moisture and snow cover are simply passive elements or whether they can affect the evolution of climate on seasonal and longer time scales. We have constructed ensembles of predictability studies using the NCAR CCM3 in which we compared the relative roles of initial surface and atmospheric conditions over the central and western U.S. GAPP region in determining the subsequent evolution of soil moisture and of snow cover. We have also made sensitivity studies with exaggerated soil moisture and snow cover anomalies in order to determine the physical processes that may be important. Results from simulations with realistic soil moisture anomalies indicate that internal climate variability may be the strongest factor, with some indication that the initial atmospheric state is also important. The initial state of soil moisture does not appear important, a result that held whether simulations were started in late winter or late spring. Model runs with exaggerated soil moisture reductions (near-desert conditions) showed a much larger effect, with warmer surface temperatures, reduced precipitation, and lower surface pressures; the latter indicating a response of the atmospheric circulation. These results suggest the possibility of a threshold effect in soil moisture, whereby an anomaly must be of a sufficient size before it can have a significant impact on the atmospheric circulation and hence climate. Results from simulations with realistic snow cover anomalies indicate that the time of year can be crucial. When introduced in late winter, these anomalies strongly affected the subsequent evolution of snow cover. When introduced in early winter, however, little or no effect is seen on the subsequent snow cover. Runs with greatly exaggerated initial snow cover indicate that the high reflectivity of snow is the most important process by which snow cover can impact climate

  11. In-Situ Quantification of Methanotrophic Activity in a Landfill Cover Soil Using Gas Push-Pull Tests

    NASA Astrophysics Data System (ADS)

    Gomez, K. E.; Gonzalez-Gil, G.; Schroth, M. H.; Zeyer, J.

    2007-12-01

    Landfills are both a major anthropogenic source and a sink for the greenhouse gas CH4. Methanogenic bacteria produce CH4 during the anaerobic digestion of landfill waste, whereas, methanotrophic bacteria consume CH4 as it is transported through a landfill cover soil. Methanotrophs are thought to be ubiquitous in soils, but typically exist in large numbers at oxic/anoxic interfaces, close to anaerobic methane sources but exposed to oxygen required for metabolism. Accurate in-situ quantification of the sink strength of methanotrophs in landfill cover soils is needed for global carbon balances and for local emissions mitigation strategies. We measured in-situ CH4 concentrations at 30, 60, and 100 cm depth at 18 evenly spaced locations across a landfill cover soil. Furthermore, we performed Gas Push-Pull Tests (GPPTs) to estimate in-situ rates of methanotrophic activity in the cover soil. The GPPT is a gas-tracer test in which a gas mixture containing CH4, O2, and non-reactive tracer gases is injected (pushed) into the soil followed by extraction (pull) from the same location. Quantification of CH4 oxidation rates is based upon comparison of the breakthrough curves of CH4 and tracer gases. We present the results of a series of GPPTs conducted at two locations in the cover soil to assess the feasibility and reproducibility of this technique to quantify methanotrophic activity. Additional GPPTs were performed with a methanotrophic inhibitor in the injection gas mixture to confirm the appropriate choice of tracers to quantify CH4 oxidation. Estimated CH4 oxidation rate constants indicate that the cover soil contains a highly active methanotrophic community.

  12. 30 CFR 280.4 - What activities are not covered by this part?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., which are covered by regulations at 30 CFR part 282; and (d) G&G exploration or G&G scientific research...? 280.4 Section 280.4 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, REGULATION, AND ENFORCEMENT... CFR part 251....

  13. Multiple microbial activity-based measures reflect effects of cover cropping and tillage on soils

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agricultural producers, conservation professionals, and policy makers are eager to learn of soil analytical techniques and data that document improvement in soil health by agricultural practices such as no-till and incorporation of cover crops. However, there is considerable uncertainty within the r...

  14. The EMT-activator ZEB1 induces bone metastasis associated genes including BMP-inhibitors

    PubMed Central

    Mock, Kerstin; Preca, Bogdan-Tiberius; Brummer, Tilman; Brabletz, Simone; Stemmler, Marc P.; Brabletz, Thomas

    2015-01-01

    Tumor cell invasion, dissemination and metastasis is triggered by an aberrant activation of epithelial-to-mesenchymal transition (EMT), often mediated by the transcription factor ZEB1. Disseminating tumor cells must acquire specific features that allow them to colonize at different organ sites. Here we identify a set of genes that is highly expressed in breast cancer bone metastasis and activated by ZEB1. This gene set includes various secreted factors, e.g. the BMP-inhibitor FST, that are described to reorganize the bone microenvironment. By inactivating BMP-signaling, BMP-inhibitors are well-known to induce osteolysis in development and disease. We here demonstrate that the expression of ZEB1 and BMP-inhibitors is correlated with bone metastasis, but not with brain or lung metastasis of breast cancer patients. In addition, we show that this correlated expression pattern is causally linked, as ZEB1 induces the expression of the BMP-inhibitors NOG, FST and CHRDL1 both by directly increasing their gene transcription, as well as by indirectly suppressing their reduction via miR-200 family members. Consequently, ZEB1 stimulates BMP-inhibitor mediated osteoclast differentiation. These findings suggest that ZEB1 is not only driving EMT, but also contributes to the formation of osteolytic bone metastases in breast cancer. PMID:25973542

  15. Estimating Cloud Cover

    ERIC Educational Resources Information Center

    Moseley, Christine

    2007-01-01

    The purpose of this activity was to help students understand the percentage of cloud cover and make more accurate cloud cover observations. Students estimated the percentage of cloud cover represented by simulated clouds and assigned a cloud cover classification to those simulations. (Contains 2 notes and 3 tables.)

  16. Be BOLD: Encouraging Girls to Include Unstructured Bouts of Physical Activity into Daily Routines

    ERIC Educational Resources Information Center

    Hill, Kory; Williams, Gwynne M.

    2014-01-01

    Adolescent girls are less active than their male counterparts and physical activity levels tend to decline as one ages. One of the goals of concerned physical educators is to promote a physically active lifestyle and to teach skills and promote behaviors that will allow students to be active both in and out of school. This article presents a…

  17. Active control of environmental noise, VIII: increasing the response to primary source changes including unpredictable noise

    NASA Astrophysics Data System (ADS)

    Wright, S. E.; Atmoko, H.; Vuksanovic, B.

    2004-07-01

    Conventional adaptive cancellation systems using traditional transverse finite impulse response (FIR) filters, together with least mean square (LMS) adaptive algorithms, well known in active noise control, are slow to adapt to primary source changes. This makes them inappropriate for cancelling rapidly changing noise, including unpredictable noise such as speech and music. Secondly, the cancelling structures require considerable computational processing effort to adapt to primary source and plant changes, particularly for multi-channel systems. This paper describes methods to increase the adaptive speed to primary source changes in large enclosed spaces and outdoor environments. A method is described that increases the response to time varying periodic noise using traditional transverse FIR filters. Here a multi-passband filter, with individual variable adaptive step sizes for each passband is automatically adjusted according to the signal level in each band. This creates a similar adaptive response for all frequencies within the total pass-band, irrespective of amplitude, minimizing the signal distortion and increasing the combined adaptive speed. Unfortunately, there is a limit to the adaptive speed using the above method as classical transverse FIR filters have a finite adaptive speed given by the stability band zero bandwidth. For rapidly changing periodic noise and unpredictable non-stationary noise, a rapid to instantaneous response is required. In this case the on-line adaptive FIR filters are dispensed with and replaced by a time domain solution that gives virtually instantaneous cancellation response (infinite adaptive speed) to primary source changes, and is computationally efficient.

  18. 45 CFR 287.130 - Can NEW Program activities include job market assessments, job creation and economic development...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Can NEW Program activities include job market assessments, job creation and economic development activities? 287.130 Section 287.130 Public Welfare... Program Design and Operations § 287.130 Can NEW Program activities include job market assessments,...

  19. 45 CFR 287.130 - Can NEW Program activities include job market assessments, job creation and economic development...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false Can NEW Program activities include job market assessments, job creation and economic development activities? 287.130 Section 287.130 Public Welfare... Program Design and Operations § 287.130 Can NEW Program activities include job market assessments,...

  20. 45 CFR 287.130 - Can NEW Program activities include job market assessments, job creation and economic development...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Can NEW Program activities include job market assessments, job creation and economic development activities? 287.130 Section 287.130 Public Welfare... Program Design and Operations § 287.130 Can NEW Program activities include job market assessments,...

  1. 45 CFR 287.130 - Can NEW Program activities include job market assessments, job creation and economic development...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true Can NEW Program activities include job market assessments, job creation and economic development activities? 287.130 Section 287.130 Public Welfare... Program Design and Operations § 287.130 Can NEW Program activities include job market assessments,...

  2. 45 CFR 287.130 - Can NEW Program activities include job market assessments, job creation and economic development...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Can NEW Program activities include job market assessments, job creation and economic development activities? 287.130 Section 287.130 Public Welfare... Program Design and Operations § 287.130 Can NEW Program activities include job market assessments,...

  3. Using Assistive Technology Adaptations To Include Students with Learning Disabilities in Cooperative Learning Activities.

    ERIC Educational Resources Information Center

    Bryant, Diane Pedrotty; Bryant, Brian R.

    1998-01-01

    Discusses a process for integrating technology adaptations for students with learning disabilities into cooperative-learning activities in terms of three components: (1) selecting adaptations; (2) monitoring use of adaptations during cooperative-learning activities; and (3) evaluating the adaptations' effectiveness. Barriers to and support systems…

  4. 50 CFR 18.111 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Walruses and Polar Bears Incidental to Oil and Gas Exploration Activities in the Chukchi Sea and Adjacent... apply to the nonlethal incidental, but not intentional, take of small numbers of Pacific walruses...

  5. 50 CFR 18.111 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Walruses and Polar Bears Incidental to Oil and Gas Exploration Activities in the Chukchi Sea and Adjacent... apply to the nonlethal incidental, but not intentional, take of small numbers of Pacific walruses...

  6. 50 CFR 18.111 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Walruses and Polar Bears Incidental to Oil and Gas Exploration Activities in the Chukchi Sea and Adjacent... apply to the nonlethal incidental, but not intentional, take of small numbers of Pacific walruses...

  7. 50 CFR 18.111 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Walruses and Polar Bears Incidental to Oil and Gas Exploration Activities in the Chukchi Sea and Adjacent... apply to the nonlethal incidental, but not intentional, take of small numbers of Pacific walruses...

  8. 50 CFR 18.111 - What specified activities does this subpart cover?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Walruses and Polar Bears Incidental to Oil and Gas Exploration Activities in the Chukchi Sea and Adjacent... apply to the nonlethal incidental, but not intentional, take of small numbers of Pacific walruses...

  9. 77 FR 29663 - Agency Information Collection Activities: Proposed Collection; Comment Request; User Fee Cover...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... product applications and supplements. DATES: Submit either electronic or written comments on the... information is necessary for the proper performance of FDA's functions, including whether the information will... drug and biologics license applications and supplements. Under this authority, pharmaceutical...

  10. Lactate Effectively Covers Energy Demands during Neuronal Network Activity in Neonatal Hippocampal Slices

    PubMed Central

    Ivanov, Anton; Mukhtarov, Marat; Bregestovski, Piotr; Zilberter, Yuri

    2011-01-01

    Although numerous experimental data indicate that lactate is efficiently used for energy by the mature brain, the direct measurements of energy metabolism parameters during neuronal network activity in early postnatal development have not been performed. Therefore, the role of lactate in the energy metabolism of neurons at this age remains unclear. In this study, we monitored field potentials and contents of oxygen and NAD(P)H in correlation with oxidative metabolism during intense network activity in the CA1 hippocampal region of neonatal brain slices. We show that in the presence of glucose, lactate is effectively utilized as an energy substrate, causing an augmentation of oxidative metabolism. Moreover, in the absence of glucose lactate is fully capable of maintaining synaptic function. Therefore, during network activity in neonatal slices, lactate can be an efficient energy substrate capable of sustaining and enhancing aerobic energy metabolism. PMID:21602909

  11. Diffractive laser beam homogenizer including a photo-active material and method of fabricating the same

    SciTech Connect

    Bayramian, Andy J; Ebbers, Christopher A; Chen, Diana C

    2014-05-20

    A method of manufacturing a plurality of diffractive optical elements includes providing a partially transmissive slide, providing a first piece of PTR glass, and directing first UV radiation through the partially transmissive slide to impinge on the first piece of PTR glass. The method also includes exposing predetermined portions of the first piece of PTR glass to the first UV radiation and thermally treating the exposed first piece of PTR glass. The method further includes providing a second piece of PTR glass and directing second UV radiation through the thermally treated first piece of PTR glass to impinge on the second piece of PTR glass. The method additionally includes exposing predetermined portions of the second piece of PTR glass to the second UV radiation, thermally treating the exposed second piece of PTR glass, and repeating providing and processing of the second piece of PTR glass using additional pieces of PTR glass.

  12. In vitro and in vivo anti-plasmodial activity of essential oils, including hinokitiol.

    PubMed

    Fujisaki, Ryuichi; Kamei, Kiyoko; Yamamura, Mariko; Nishiya, Hajime; Inouye, Shigeharu; Takahashi, Miki; Abe, Shigeru

    2012-03-01

    Abstract. The anti-plasmodial activity of 47 essential oils and 10 of their constituents were screened for in vitro activity against Plasmodium falciparum. Five of these essential oils (sandalwood, caraway, monarda, nutmeg, and Thujopsis dolabrata var. hondai) and 2 constituents (thymoquinone and hinokitiol) were found to be active against P. falciparum in vitro, with 50% inhibitory concentration (IC50) values equal to or less than 1.0 microg/ml. Furthermore, in vivo analysis using a rodent model confirmed the anti-plasmodial potential of subcutaneously administered sandalwood oil, and percutaneously administered hinokitiol and caraway oil against rodent P. berghei. Notably, these oils showed no efficacy when administered orally, intraperitoneally or intravenously. Caraway oil and hinokitiol dissolved in carrier oil, applied to the skin of hairless mice caused high levels in the blood, with concentrations exceeding their IC50 values. PMID:23082579

  13. In vitro and in vivo anti-plasmodial activity of essential oils, including hinokitiol.

    PubMed

    Fujisaki, Ryuichi; Kamei, Kiyoko; Yamamura, Mariko; Nishiya, Hajime; Inouye, Shigeharu; Takahashi, Miki; Abe, Shigeru

    2012-03-01

    Abstract. The anti-plasmodial activity of 47 essential oils and 10 of their constituents were screened for in vitro activity against Plasmodium falciparum. Five of these essential oils (sandalwood, caraway, monarda, nutmeg, and Thujopsis dolabrata var. hondai) and 2 constituents (thymoquinone and hinokitiol) were found to be active against P. falciparum in vitro, with 50% inhibitory concentration (IC50) values equal to or less than 1.0 microg/ml. Furthermore, in vivo analysis using a rodent model confirmed the anti-plasmodial potential of subcutaneously administered sandalwood oil, and percutaneously administered hinokitiol and caraway oil against rodent P. berghei. Notably, these oils showed no efficacy when administered orally, intraperitoneally or intravenously. Caraway oil and hinokitiol dissolved in carrier oil, applied to the skin of hairless mice caused high levels in the blood, with concentrations exceeding their IC50 values.

  14. Electrode including porous particles with embedded active material for use in a secondary electrochemical cell

    DOEpatents

    Vissers, Donald R.; Nelson, Paul A.; Kaun, Thomas D.; Tomczuk, Zygmunt

    1978-04-25

    Particles of carbonaceous matrices containing embedded electrode active material are prepared for vibratory loading within a porous electrically conductive substrate. In preparing the particles, active materials such as metal chalcogenides, solid alloys of alkali or alkaline earth metals along with other metals and their oxides in powdered or particulate form are blended with a thermosetting resin and particles of a volatile to form a paste mixture. The paste is heated to a temperature at which the volatile transforms into vapor to impart porosity at about the same time as the resin begins to cure into a rigid, solid structure. The solid structure is then comminuted into porous, carbonaceous particles with the embedded active material.

  15. Activity concentration of natural radionuclides and radon and thoron exhalation rates in rocks used as decorative wall coverings in Japan.

    PubMed

    Iwaoka, Kazuki; Hosoda, Masahiro; Tabe, Hiroyuki; Ishikawa, Tetsuo; Tokonami, Shinji; Yonehara, Hidenori

    2013-01-01

    In Japan, many dwellings have decorative wall coverings made from granite, andesite, tuff, gabbro, and marble. However, information regarding activity concentrations and radon (Rn) and thoron (Rn) exhalation rates for such rocks is very scarce. Therefore, samples of the granite, andesite, tuff, and marble that are used as wall coverings in Japan were collected from mining companies, and their activity concentrations and Rn and Rn exhalation rates were measured. Dose estimations for inhabitants living in houses built with these materials were also carried out. The activity concentration of natural radionuclides in all the materials was lower than the critical values described by the International Atomic Energy Agency (IAEA) (10,000 Bq kg for K and 1,000 Bq kg for all other radionuclides of natural origin). The maximum values of Rn and Rn mass exhalation rates for the granite samples were 0.12 and 430 mBq kg s, and those for the area exhalation rates were 1.8 and 6300 mBq m s, respectively; these values are higher than those for other samples. The maximum value of effective doses to inhabitants was 0.68 mSv y, which is lower than the intervention exemption level (1 mSv y) given in the International Commission on Radiological Protection (ICRP) Publication 82.

  16. Activity concentration of natural radionuclides and radon and thoron exhalation rates in rocks used as decorative wall coverings in Japan.

    PubMed

    Iwaoka, Kazuki; Hosoda, Masahiro; Tabe, Hiroyuki; Ishikawa, Tetsuo; Tokonami, Shinji; Yonehara, Hidenori

    2013-01-01

    In Japan, many dwellings have decorative wall coverings made from granite, andesite, tuff, gabbro, and marble. However, information regarding activity concentrations and radon (Rn) and thoron (Rn) exhalation rates for such rocks is very scarce. Therefore, samples of the granite, andesite, tuff, and marble that are used as wall coverings in Japan were collected from mining companies, and their activity concentrations and Rn and Rn exhalation rates were measured. Dose estimations for inhabitants living in houses built with these materials were also carried out. The activity concentration of natural radionuclides in all the materials was lower than the critical values described by the International Atomic Energy Agency (IAEA) (10,000 Bq kg for K and 1,000 Bq kg for all other radionuclides of natural origin). The maximum values of Rn and Rn mass exhalation rates for the granite samples were 0.12 and 430 mBq kg s, and those for the area exhalation rates were 1.8 and 6300 mBq m s, respectively; these values are higher than those for other samples. The maximum value of effective doses to inhabitants was 0.68 mSv y, which is lower than the intervention exemption level (1 mSv y) given in the International Commission on Radiological Protection (ICRP) Publication 82. PMID:23192085

  17. 17 CFR Appendix A to Part 255 - Reporting and Recordkeeping Requirements for Covered Trading Activities

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... positions: risk factors with respect to the related commodities set out in 17 CFR 20.2, the maturity of the... and Position Limits and Usage; • Risk Factor Sensitivities; • Value-at-Risk and Stress VaR... below, including “Risk Factor Sensitivities” and “Value-at-Risk and Stress Value-at-Risk,” relate to...

  18. Physical Activity Programs in Higher Education: Modifying Net/Wall Games to Include Individuals with Disabilities

    ERIC Educational Resources Information Center

    Braga, Luciana; Tracy, Julia F.; Taliaferro, Andrea R.

    2015-01-01

    The growing number of students with disabilities in higher education settings has presented challenges for instructors with regards to appropriate inclusion. Concerning physical activity courses in higher education, instructors may not have the knowledge or resources to make modifications and accommodations that will ultimately result in…

  19. Observing a fictitious stressful event: haematological changes, including circulating leukocyte activation.

    PubMed

    Mian, Rubina; Shelton-Rayner, Graham; Harkin, Brendan; Williams, Paul

    2003-03-01

    The aim of this study was to assess the effect of watching a psychological stressful event on the activation of leukocytes in healthy human volunteers. Blood samples were obtained from 32 healthy male and female subjects aged between 20 and 26 years before, during and after either watching an 83-minute horror film that none of the subjects had previously seen (The Texas Chainsaw Massacre, 1974) or by sitting quietly in a room (control group). Total differential cell counts, leukocyte activation as measured by the nitroblue tetrazolium (NBT) test, heart rate and blood pressure (BP) measurements were taken at defined time points. There were significant increases in peripheral circulating leukocytes, the number of activated circulating leukocytes, haemoglobin (Hb) concentration and haematocrit (Hct) in response to the stressor. These were accompanied by significant increases in heart rate, systolic and diastolic BP (P<0.05 from baseline). This is the first reported study on the effects of observing a psychologically stressful, albeit fictitious event on circulating leukocyte numbers and the state of leukocyte activation as determined by the nitrotetrazolium test.

  20. Sixty Minutes of Physical Activity per Day Included within Preschool Academic Lessons Improves Early Literacy

    ERIC Educational Resources Information Center

    Kirk, Stacie M.; Kirk, Erik P.

    2016-01-01

    Background: The effects of increases in physical activity (PA) on early literacy skills in preschool children are not known. Methods: Fifty-four African-American preschool children from a low socioeconomic urban Head Start participated over 8 months. A 2-group, quasi-experimental design was used with one preschool site participating in the PA…

  1. Population and Human Development: A Course Curriculum Including Lesson Plans, Activities and Bibliography.

    ERIC Educational Resources Information Center

    Murphy, Elaine M.

    This course outline suggests materials and learning activities on the interrelated causes and consequences of population growth and other population concerns. Designed to educate general college audiences, it is also intended for use as a preservice course for teachers. In addition, the course can be modified for high school students. The course…

  2. 7 CFR 981.441 - Credit for market promotion activities, including paid advertising.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGRICULTURE ALMONDS GROWN IN CALIFORNIA Administrative Rules and Regulations § 981.441 Credit for market... each activity shall be to promote the sale, consumption or use of California almonds, and nothing... in California almond growing counties with more than 1,000 bearing acres: Provided, That...

  3. 7 CFR 981.441 - Credit for market promotion activities, including paid advertising.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AGRICULTURE ALMONDS GROWN IN CALIFORNIA Administrative Rules and Regulations § 981.441 Credit for market... each activity shall be to promote the sale, consumption or use of California almonds, and nothing... in California almond growing counties with more than 1,000 bearing acres: Provided, That...

  4. 7 CFR 981.441 - Credit for market promotion activities, including paid advertising.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGRICULTURE ALMONDS GROWN IN CALIFORNIA Administrative Rules and Regulations § 981.441 Credit for market... each activity shall be to promote the sale, consumption or use of California almonds, and nothing... in California almond growing counties with more than 1,000 bearing acres: Provided, That...

  5. 7 CFR 981.441 - Credit for market promotion activities, including paid advertising.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE ALMONDS GROWN IN CALIFORNIA Administrative Rules and Regulations § 981.441 Credit for market... each activity shall be to promote the sale, consumption or use of California almonds, and nothing... in California almond growing counties with more than 1,000 bearing acres: Provided, That...

  6. Backyards and Butterflies: Ways to Include Children with Disabilities in Outdoor Activities.

    ERIC Educational Resources Information Center

    Greenstein, Doreen; And Others

    This sourcebook is designed for children, parents, and families, detailing ideas for outdoor play and learning activities, with emphasis on involving children with disabilities in outdoor play. A rural perspective permeates the guide, although each chapter contains ideas for making outdoor environments more accessible and safer for all children,…

  7. Beyond Right or Wrong: Challenges of Including Creative Design Activities in the Classroom

    ERIC Educational Resources Information Center

    Brennan, Karen

    2015-01-01

    In this article, we explore challenges encountered by K-12 educators in establishing classroom cultures that support creative learning activities with the Scratch programming language. Providing opportunities for students to understand and to build capacities for creative work was described by many of the teachers that we interviewed as a central…

  8. [Effect of the vegetative cover on the biological activity of the soil of Chaco Arido].

    PubMed

    Abril, A; Acosta, M; Bachmeier, O; Rollan, A

    1993-01-01

    Vegetation plays a primal role in arid ecosystems, since it creates microclimate conditions that moderate the characteristics of the region whereby the rational use of vegetal resources is fundamental. Felling, clearing and overgrazing lead to decrease in organic contribution and stimulate soil compaction, causing an alteration of microbial activity, with losses in nutrient turnover. The global biological activity is a soil parameter easy to obtain and indicates the presence and diversity of soil life as well as substrate availability and is useful in order to characterize soil potential fertility. This work was carried out in Natural Forest Reserve Chancaní, Province of Córdoba (Argentina), which is representative of Argentine Dry Chaco. Dominant tree species are: Prosopis flexuosa and Aspidosperma quebracho blanco. The global biological activity (GBA) was measured along one year, under trees, under shrubs and in interspaces. Soil samples were taken monthly from plots with four management systems: 1) forest, ii) selective clearing (only dominant species remain), iii) bush (clearing invaded by Larrea sp) and iv) grazing (cleared area, neither trees nor shrubs). GBA was evaluated using the CO2 release method, after ten days of incubation. It is concluded that in the plots with grasses and under the trees GBA was higher than with other treatments. The lesser GBA was detected in bushes and interspaces. All differences were more prominent during extreme temperature months. No significant difference between both species of dominant trees was observed. PMID:8210407

  9. Space Resources for Teachers: Biology, Including Suggestions for Classroom Activities and Laboratory Experiments.

    ERIC Educational Resources Information Center

    Lee, Tom E.; And Others

    This compilation of resource units concerns the latest developments in space biology. Some of the topics included are oxygen consumption, temperature, radiation, rhythms, weightlessness, acceleration and vibration stress, toxicity, and sensory and perceptual problems. Many of the topics are interdisciplinary and relate biology, physiology,…

  10. 7 CFR 981.441 - Credit for market promotion activities, including paid advertising.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... relations purposes, including E-commerce (mail ordering through the Internet): Provided, That Credit-Back...-Back is requested. (e) The following requirements shall apply to Credit-Back for all promotional... and Canada, paragraph (e)(5) of this section shall also apply. (2) The clear and evident purpose...

  11. In vitro bactericidal activity of aminoglycosides, including the next-generation drug plazomicin, against Brucella spp.

    PubMed

    Olsen, Steven C; Carlson, Steve A

    2015-01-01

    Plazomicin is a next-generation aminoglycoside with a potentially unique set of clinical characteristics compared with other aminoglycosides. This study assessed the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of plazomicin against 15 clinical isolates as well as three reference strains representing Brucella abortus, Brucella melitensis and Brucella suis. These data were compared with those obtained for six other aminoglycosides and two aminocyclitols. Plazomicin and gentamicin were the only drugs demonstrating bactericidal activity towards two of the three Brucella spp., whilst plazomicin was the only drug exhibiting bactericidal activity against B. suis. This is the first study to assess the bactericidal nature of plazomicin against Brucella spp. in vitro. PMID:25459738

  12. Active layer thermal regime at different vegetation covers at Lions Rump, King George Island, Maritime Antarctica

    NASA Astrophysics Data System (ADS)

    Almeida, Ivan C. C.; Schaefer, Carlos Ernesto G. R.; Fernandes, Raphael B. A.; Pereira, Thiago T. C.; Nieuwendam, Alexandre; Pereira, Antônio Batista

    2014-11-01

    Climate change impacts the biotic and abiotic components of polar ecosystems, affecting the stability of permafrost, active layer thickness, vegetation, and soil. This paper describes the active layer thermal regimes of two adjacent shallow boreholes, under the same soil but with two different vegetations. The study is location in Lions Rump, at King George Island, Maritime Antarctic, one of the most sensitive regions to climate change, located near the climatic limit of Antarctic permafrost. Both sites are a Turbic Cambic Cryosol formed on andesitic basalt, one under moss vegetation (Andreaea gainii, at 85 m a.s.l.) and another under lichen (Usnea sp., at 86 m a.s.l.), located 10 m apart. Ground temperature at same depths (10, 30 and 80 cm), water content at 80 cm depth and air temperature were recorded hourly between March 2009 and February 2011. The two sites showed significant differences in mean annual ground temperature for all depths. The lichen site showed a higher soil temperature amplitude compared to the moss site, with ground surface (10 cm) showing the highest daily temperature in January 2011 (7.3 °C) and the lowest daily temperature in August (- 16.5 °C). The soil temperature at the lichen site closely followed the air temperature trend. The moss site showed a higher water content at the bottommost layer, consistent with the water-saturated, low landscape position. The observed thermal buffering effect under mosses is primarily associated with higher moisture onsite, but a longer duration of the snowpack (not monitored) may also have influenced the results. Active layer thickness was approximately 150 cm at low-lying moss site, and 120 cm at well-drained lichen site. This allows to classify these soils as Cryosols (WRB) or Gelisols (Soil Taxonomy), with evident turbic features.

  13. Activity of faropenem tested against Neisseria gonorrhoeae isolates including fluoroquinolone-resistant strains.

    PubMed

    Jones, Ronald N; Critchley, Ian A; Whittington, William L H; Janjic, Nebojsa; Pottumarthy, Sudha

    2005-12-01

    We evaluated the anti-gonococcal potency of faropenem along with 7 comparator reference antimicrobials against a preselected collection of clinical isolates. The 265 isolates were inclusive of 2 subsets: 1) 76 well-characterized resistant phenotypes of gonococcal strains (53 quinolone-resistant strains--31 with documented quinolone resistance-determining region changes from Japan, 15 strains resistant to penicillin and tetracycline, and 8 strains with intermediate susceptibility to penicillin) and 2) 189 recent isolates from clinical specimens in 2004 from 6 states across the United States where quinolone resistance is prevalent. Activity of faropenem was adversely affected by l-cysteine hydrochloride in IsoVitaleX (4-fold increase in [minimal inhibitory concentration] MIC50; 0.06 versus 0.25 microg/mL). The rank order of potency of the antimicrobials for the entire collection was ceftriaxone (MIC90, 0.06 microg/mL) > faropenem (0.25 microg/mL) > azithromycin (0.5 microg/mL) > cefuroxime (1 microg/mL) > tetracycline (2 microg/mL) > penicillin = ciprofloxacin = levofloxacin (4 microg/mL). Using MIC90 for comparison, faropenem was 4-fold more potent than cefuroxime (0.25 versus 1 microg/mL), but was 4-fold less active than ceftriaxone (0.25 versus 0.06 microg/mL). Although the activity of faropenem was not affected by either penicillinase production (MIC90, 0.12 microg/mL, penicillinase-positive) or increasing ciprofloxacin MIC (0.25 microg/mL, ciprofloxacin-resistant), increasing penicillin MIC was associated with an increase in MIC90 values (0.016 microg/mL for penicillin-susceptible to 0.25 microg/mL for penicillin-resistant strains). Among the recent (2004) clinical gonococcal isolates tested, reduced susceptibility to penicillins, tetracycline, and fluoroquinolones was high (28.0-94.2%). Geographic distribution of the endemic resistance rates of gonococci varied considerably, with 16.7-66.7% of the gonococcal isolates being ciprofloxacin-resistant in Oregon

  14. Phytophthora infestans Has a Plethora of Phospholipase D Enzymes Including a Subclass That Has Extracellular Activity

    PubMed Central

    Meijer, Harold J. G.; Hassen, Hussen Harrun; Govers, Francine

    2011-01-01

    In eukaryotes phospholipase D (PLD) is involved in many cellular processes. Currently little is known about PLDs in oomycetes. Here we report that the oomycete plant pathogen Phytophthora infestans has a large repertoire of PLDs divided over six subfamilies: PXPH-PLD, PXTM-PLD, TM-PLD, PLD-likes, and type A and B sPLD-likes. Since the latter have signal peptides we developed a method using metabolically labelled phospholipids to monitor if P. infestans secretes PLD. In extracellular medium of ten P. infestans strains PLD activity was detected as demonstrated by the production of phosphatidic acid and the PLD specific marker phosphatidylalcohol. PMID:21423760

  15. Phytophthora infestans has a plethora of phospholipase D enzymes including a subclass that has extracellular activity.

    PubMed

    Meijer, Harold J G; Hassen, Hussen Harrun; Govers, Francine

    2011-01-01

    In eukaryotes phospholipase D (PLD) is involved in many cellular processes. Currently little is known about PLDs in oomycetes. Here we report that the oomycete plant pathogen Phytophthora infestans has a large repertoire of PLDs divided over six subfamilies: PXPH-PLD, PXTM-PLD, TM-PLD, PLD-likes, and type A and B sPLD-likes. Since the latter have signal peptides we developed a method using metabolically labelled phospholipids to monitor if P. infestans secretes PLD. In extracellular medium of ten P. infestans strains PLD activity was detected as demonstrated by the production of phosphatidic acid and the PLD specific marker phosphatidylalcohol. PMID:21423760

  16. Brutality under cover of ambiguity: activating, perpetuating, and deactivating covert retributivism.

    PubMed

    Fincher, Katrina M; Tetlock, Philip E

    2015-05-01

    Five studies tested four hypotheses on the drivers of punitive judgments. Study 1 showed that people imposed covertly retributivist physical punishments on extreme norm violators when they could plausibly deny that is what they were doing (attributional ambiguity). Studies 2 and 3 showed that covert retributivism could be suppressed by subtle accountability manipulations that cue people to the possibility that they might be under scrutiny. Studies 4 and 5 showed how covert retributivism can become self-sustaining by biasing the lessons people learn from experience. Covert retributivists did not scale back punitiveness in response to feedback that the justice system makes false-conviction errors but they did ramp up punitiveness in response to feedback that the system makes false-acquittal errors. Taken together, the results underscore the paradoxical nature of covert retributivism: It is easily activated by plausible deniability and persistent in the face of false-conviction feedback but also easily deactivated by minimalist forms of accountability.

  17. A Methodology for Post Operational Clean Out of a Highly Active Facility Including Solids Behaviour - 12386

    SciTech Connect

    Edmondson, Michael J.; Ward, Tracy R.; Maxwell, Lisa J.

    2012-07-01

    The Highly Active Liquor Evaporation and Storage (HALES) plant at Sellafield handles acidic fission product containing liquor with typical activities of the order of 18x10{sup 9} Bq/ml. A strategy experimental feedback approach has been used to establish a wash regime for the Post Operational Clean Out (POCO) of the oldest storage tanks for this liquor. Two different wash reagents have been identified as being potentially suitable for removal of acid insoluble fission product precipitates. Ammonium carbamate and sodium carbonate yield similar products during the proposed wash cycle. The proposed wash reagents provide dissolution of caesium phosphomolybdate (CPM) and zirconium molybdate (ZM) solid phases but yields a fine, mobile precipitate of metal carbonates from the Highly Active Liquor (HAL) supernate. Addition of nitric acid to the wash effluent can cause CPM to precipitate where there is sufficient caesium and phosphorous available. Where they are not present (from ZM dissolution) the nitric acid addition initially produces a nitrate precipitate which then re-dissolves, along with the metal carbonates, to give a solid-free solution. The different behaviour of the two solids during the wash cycle has led to the proposal for an amended flowsheet. Additional studies on the potential to change the morphology of crystallising ZM have presented opportunities for changing the rheology of ZM sediments through doping with tellurium or particular organic acids. Two different wash reagents have been identified as being potentially suitable for the POCO of HALES Oldside HASTs. AC and SC both yield similar products during the proposed wash cycle. However, the different behaviour of the two principle HAL solids, CPM and ZM, during the wash cycle has led to the proposal for an amended flowsheet. Additional studies on the potential to change the morphology of crystallising ZM have presented opportunities for changing its rheology through doping with tellurium or certain

  18. Nuclear Rocket Test Facility Decommissioning Including Controlled Explosive Demolition of a Neutron-Activated Shield Wall

    SciTech Connect

    Michael Kruzic

    2007-09-01

    Located in Area 25 of the Nevada Test Site, the Test Cell A Facility was used in the 1960s for the testing of nuclear rocket engines, as part of the Nuclear Rocket Development Program. The facility was decontaminated and decommissioned (D&D) in 2005 using the Streamlined Approach For Environmental Restoration (SAFER) process, under the Federal Facilities Agreement and Consent Order (FFACO). Utilities and process piping were verified void of contents, hazardous materials were removed, concrete with removable contamination decontaminated, large sections mechanically demolished, and the remaining five-foot, five-inch thick radiologically-activated reinforced concrete shield wall demolished using open-air controlled explosive demolition (CED). CED of the shield wall was closely monitored and resulted in no radiological exposure or atmospheric release.

  19. LIPID PEROXIDATION GENERATES BIOLOGICALLY ACTIVE PHOSPHOLIPIDS INCLUDING OXIDATIVELY N-MODIFIED PHOSPHOLIPIDS

    PubMed Central

    Davies, Sean S.; Guo, Lilu

    2014-01-01

    Peroxidation of membranes and lipoproteins converts “inert” phospholipids into a plethora of oxidatively modified phospholipids (oxPL) that can act as signaling molecules. In this review, we will discuss four major classes of oxPL: mildly oxygenated phospholipids, phospholipids with oxidatively truncated acyl chains, phospholipids with cyclized acyl chains, and phospholipids that have been oxidatively N-modified on their headgroups by reactive lipid species. For each class of oxPL we will review the chemical mechanisms of their formation, the evidence for their formation in biological samples, the biological activities and signaling pathways associated with them, and the catabolic pathways for their elimination. We will end by briefly highlighting some of the critical questions that remain about the role of oxPL in physiology and disease. PMID:24704586

  20. Cover Picture.

    PubMed

    Das, Sanjoy K.; Mallet, Jean-Maurice; Esnault, Jacques; Driguez, Pierre-Alexandre; Duchaussoy, Philippe; Sizun, Philippe; Hérault, Jean-Pascal; Herbert, Jean-Marc; Petitou, Maurice; Sinaÿ, Pierre

    2001-05-01

    The cover picture shows how thrombosis occurs in the deep veins of the lower limbs. Stasis, which results from slow and turbulent blood flow, combined with hypercoagulation, caused, for example, by a surgical procedure, may result in thrombus formation. The synthetic sulfated pentasaccharide shown in part is a potent antithrombotic compound that exerts its effect by activation of the plasma protein antithrombin III. Conformationally locked monosaccharides have now been synthesized to demonstrate that L-iduronic acid, one part of the pentasaccharide, must adopt an unusual distorted conformation to activate antithrombin III. Such conformational effects might be relevant in explaining the unique biological properties of glycosaminoglycans that contain L-iduronic acid. In the background of the picture, a flight of vampire bats is attracted by the pentasaccharide. Vampire was the name given to South American blood-sucking bats (Latin name: desmodus rotundus) in 1761 by the French naturalist Georges Louis Leclerc Comte de Buffon (1707-1788). These bats are known to attack cattle and, very rarely, sleeping human beings. Although their saliva has been shown to contain an anticoagulant compound, they would also be happy to benefit from the pentasaccharide mentioned above, to suck the blood out of the vein more easily. More details about this compound which would be helpful to vampire bats are reported by Petitou, Sinaÿ et al. on p. 1670 ff.

  1. Include Passive Solar in Your Renovations.

    ERIC Educational Resources Information Center

    Bender, Gerald F.; Probasco, Jack F.

    1981-01-01

    A checklist covers potential energy saving modifications in a building scheduled for renovation, and includes suggestions for room utilization, landscaping, and building envelope, solar control, and active system modifications. (Author)

  2. Design of a high-lift experiment in water including active flow control

    NASA Astrophysics Data System (ADS)

    Beutel, T.; Sattler, S.; El Sayed, Y.; Schwerter, M.; Zander, M.; Büttgenbach, S.; Leester-Schädel, M.; Radespiel, R.; Sinapius, M.; Wierach, P.

    2014-07-01

    This paper describes the structural design of an active flow-control experiment. The aim of the experiment is to investigate the increase in efficiency of an internally blown Coanda flap using unsteady blowing. The system uses tailor-made microelectromechanical (MEMS) pressure sensors to determine the state of the oncoming flow and an actuated lip to regulate the mass flow and velocity of a stream near a wall over the internally blown flap. Sensors and actuators are integrated into a highly loaded system that is extremely compact. The sensors are connected to a bus system that feeds the data into a real-time control system. The piezoelectric actuators using the d 33 effect at a comparable low voltage of 120 V are integrated into a lip that controls the blowout slot height. The system is designed for closed-loop control that efficiently avoids flow separation on the Coanda flap. The setup is designed for water-tunnel experiments in order to reduce the free-stream velocity and the system’s control frequency by a factor of 10 compared with that in air. This paper outlines the function and verification of the system’s main components and their development.

  3. Fatty acid-releasing activities in Sinorhizobium meliloti include unusual diacylglycerol lipase

    PubMed Central

    Sahonero-Canavesi, Diana X.; Sohlenkamp, Christian; Sandoval-Calderón, Mario; Lamsa, Anne; Pogliano, Kit; López-Lara, Isabel M.; Geiger, Otto

    2016-01-01

    Summary Phospholipids are well known for their membrane forming properties and thereby delimit any cell from the exterior world. In addition, membrane phospholipids can act as precursors for signals and other biomolecules during their turnover. Little is known about phospholipid signalling, turnover and remodelling in bacteria. Recently, we showed that a FadD-deficient mutant of Sinorhizobium meliloti, unable to convert free fatty acids to their coenzyme A derivatives, accumulates free fatty acids during the stationary phase of growth. Enzymatic activities responsible for the generation of these free fatty acids were unknown in rhizobia. Searching the genome of S. meliloti, we identified a potential lysophospholipase (SMc04041) and two predicted patatin-like phospholipases A (SMc00930, SMc01003). Although SMc00930 as well as SMc01003 contribute to the release of free fatty acids in S. meliloti, neither one can use phospholipids as substrates. Here we show that SMc01003 converts diacylglycerol to monoacylglycerol and a fatty acid, and that monoacylglycerol can be further degraded by SMc01003 to another fatty acid and glycerol. A SMc01003-deficient mutant of S. meliloti transiently accumulates diacylglycerol, suggesting that SMc01003 also acts as diacylglycerol lipase (DglA) in its native background. Expression of the DglA lipase in Escherichia coli causes lysis of cells in stationary phase of growth. PMID:25711932

  4. Fatty acid-releasing activities in Sinorhizobium meliloti include unusual diacylglycerol lipase.

    PubMed

    Sahonero-Canavesi, Diana X; Sohlenkamp, Christian; Sandoval-Calderón, Mario; Lamsa, Anne; Pogliano, Kit; López-Lara, Isabel M; Geiger, Otto

    2015-09-01

    Phospholipids are well known for their membrane-forming properties and thereby delimit any cell from the exterior world. In addition, membrane phospholipids can act as precursors for signals and other biomolecules during their turnover. Little is known about phospholipid signalling, turnover and remodelling in bacteria. Recently, we showed that a FadD-deficient mutant of Sinorhizobium meliloti, unable to convert free fatty acids to their coenzyme A derivatives, accumulates free fatty acids during the stationary phase of growth. Enzymatic activities responsible for the generation of these free fatty acids were unknown in rhizobia. Searching the genome of S. meliloti, we identified a potential lysophospholipase (SMc04041) and two predicted patatin-like phospholipases A (SMc00930, SMc01003). Although SMc00930 as well as SMc01003 contribute to the release of free fatty acids in S. meliloti, neither one can use phospholipids as substrates. Here we show that SMc01003 converts diacylglycerol to monoacylglycerol and a fatty acid, and that monoacylglycerol can be further degraded by SMc01003 to another fatty acid and glycerol. A SMc01003-deficient mutant of S. meliloti transiently accumulates diacylglycerol, suggesting that SMc01003 also acts as diacylglycerol lipase (DglA) in its native background. Expression of the DglA lipase in Escherichia coli causes lysis of cells in stationary phase of growth.

  5. Modeling the development of biofilm density including active bacteria, inert biomass, and extracellular polymeric substances.

    PubMed

    Laspidou, Chrysi S; Rittmann, Bruce E

    2004-01-01

    We present the unified multi-component cellular automaton (UMCCA) model, which predicts quantitatively the development of the biofilm's composite density for three biofilm components: active bacteria, inert or dead biomass, and extracellular polymeric substances. The model also describes the concentrations of three soluble organic components (soluble substrate and two types of soluble microbial products) and oxygen. The UMCCA model is a hybrid discrete-differential mathematical model and introduces the novel feature of biofilm consolidation. Our hypothesis is that the fluid over the biofilm creates pressures and vibrations that cause the biofilm to consolidate, or pack itself to a higher density over time. Each biofilm compartment in the model output consolidates to a different degree that depends on the age of its biomass. The UMCCA model also adds a cellular automaton algorithm that identifies the path of least resistance and directly moves excess biomass along that path, thereby ensuring that the excess biomass is distributed efficiently. A companion paper illustrates the trends that the UMCCA model is able to represent and shows a comparison with experimental results. PMID:15276752

  6. Ozone control of biological activity during Earth's history, including the KT catastrophe

    NASA Technical Reports Server (NTRS)

    Sheldon, W. R.

    1994-01-01

    There have been brief periods since the beginning of the Cambrian some 600 m.y. ago when mass extinctions destroyed a significant fraction of living species. The most widely studied of these events is the catastrophe at the KT boundary that ended the long dominance of the dinosaurs. In addition to mass extinctions, there is another profound discontinuity in the history of Earth's biota, the explosion of life at the end of the Precambrian era which is an episode that is not explained well at all. For some 3 b.y. before the Cambrian, life had been present on Earth, but maintained a low level of activity which is an aspect of the biota that is puzzling, especially during the last two-thirds of that period. During the last 2 b.y. before the Cambrian, conditions at the Earth's surface were suitable for a burgeoning of the biota, according to most criteria: the oceans neither boiled nor were fozen solid during this time, and the atmosphere contained sufficient O for the development of animals. The purpose of this paper is to suggest that mass extinctions and the lackluster behavior of the Precambrian biota share a common cause: an inadequate amount of ozone in the atmosphere.

  7. Ground Penetrating Radar (GPR) Imaging to Distinguish Active from Inactive Sinkholes in Covered Karst Terrain: Results from Field Data and 3D FDTD Modeling

    NASA Astrophysics Data System (ADS)

    Gooch, B. T.; Kruse, S. E.

    2009-12-01

    Ground penetrating radar (GPR) is widely used to identify locations of sinkholes in covered karst terrain in Florida. Some sinkholes serve as hydraulic conduits between the surficial and underlying aquifers. Their role is critical in determining the surficial aquifer response to pumping in deeper aquifers. Improved methods for discriminating between hydraulically active sinkholes and plugged sinkholes could help regional water management. In the covered karst of west-central Florida a clay-rich weathering horizon forms over the limestone. The clay-rich layer is in turn overlain by surficial sands. Ground penetrating radar profiles typically show a strong reflector from the top of clay-rich horizon as well as internal layering within sands. Active sinkholes are expected to have sandy conduits that broach the clay layer, and perhaps layering in the overlying sand indicative of ongoing subsidence. Three dimensional simulations of GPR profiles over sinkhole with and without conduits were run with the finite-difference time-domain (FDTD) program GprMax. Results from the synthetic surveys were then processed with standard techniques, including migration. The modeling confirms that conduits appear in GPR records primarily as gaps in the return from the clay layer. The modeling also shows that non-traditional survey geometries (varying antenna spacing and orientation) are unlikely to recover more information than traditional proximal transmitter-receiver separation. We also examine GPR profiles and 3D grids over a set of active and inactive sinkholes in Tampa, Florida. Preliminary analysis suggests that active sinks may present more identifiable gaps in the overlying clay layer, but consistent differences in structure of active and inactive sinkholes are not easily discerned. Other geophysical methods may prove to be more helpful in discerning the presence or absence of active conduits in these situations.

  8. Radio Properties of Low Redshift Broad Line Active Galactic Nuclei Including Multiple Component Radio Sources

    NASA Astrophysics Data System (ADS)

    Rafter, Stephen E.

    2010-01-01

    We present results on the radio properties of a low redshift (z < 0.35) sample of 8434 broad line active galactic nuclei (AGNs) from the Sloan Digital Sky Survey after correlating the optical sources with radio sources in the Faint Images of the Radio Sky at Twenty-Centimeters survey. We find that 10% of our sample has radio emission < 4" away from the optical counterpart (core-only sources), and 1% has significant extended emission that must be taken into account when calculating the total radio luminosity (multi-component sources). Association of the extended radio emission is established by the proximity to the optical source, physical connection of jets and lobes, or large scale symmetry like in classic FRIIs. From these data we find an FRI/FRII luminosity dividing line like that found by Fanaroff & Riley (1974), where we use our core-only sources as proxies for FRIs, and our multi-component sources for the FRIIs. We find a bimodal distribution for the radio loudness (R = L(radio)/L(opt)) where the lower radio luminosity core-only sources appear as a population separate from the multi-component extended sources, compared with no evidence for bimodality when just the core-only sources are used. We also find that a log(R) value of 1.75 is well suited to separate the FRIs from the FRIIs, and that the R bimodality seen here is really a manifestation of the FRI/FRII break originally found by Fanaroff & Riley (1974). We find modest trends in the radio loud fraction as a function of Eddington ratio and black hole mass, where the fraction of RL AGNs decreases with increasing Eddington ratio, and increases when the black hole mass is above 2 x 108 solar masses.

  9. Should Physical Activity Be Included in Nutrition Education? A Comparison of Nutrition Outcomes with and without In-Class Activities

    ERIC Educational Resources Information Center

    Palmer-Keenan, Debra M.; Corda, Kirsten

    2014-01-01

    Limited-resource adults' dietary intakes and nutrition behaviors improve as a result of Expanded Food and Nutrition Education Program (EFNEP)/Supplemental Nutrition Assistance Program Education (SNAP-Ed) participation; however, physical activity education is needed for improved health. The experimental study reported here assessed if spending…

  10. Development of an oregano-based ointment with anti-microbial activity including activity against methicillin-resistant Staphlococcus aureus.

    PubMed

    Eng, William; Norman, Robert

    2010-04-01

    Increasing antibiotic resistance has prompted a search for new compounds with anti-microbial activity. In the authors' previous study, oregano extract was identified as one of the most potent anti-microbial compounds. The disk diffusion method was employed to assess the degree of inhibition against various microorganisms, and the bacteriostatic or bactericidal mechanism of action. Disk diffusion studies showed that oregano was found to be bacteriostatic for Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus, (MRSA) but bacteriocidal for seven other microorganisms. Pseudomonas aeruginosa could not be inhibited by oregano. An ointment consisting of 1-10% oregano could inhibit most organisms except for Proteus mirabilis and Proteus vulgaris, which required 20% and Pseudomonas which could not be inhibited even at the highest concentration of 80%. Oregano extracts can be formulated into an ointment that shows broad antimicrobial activity. Additional testing to assess tissue toxicity and other adverse reactions would be needed prior to human testing.

  11. Covering Politics.

    ERIC Educational Resources Information Center

    Gruber, Ryan; Wind, Andrew; Trevidi, Neema

    2000-01-01

    Presents four articles considering: (1) the media's role in the coverage of politics; (2) the influence of photography particularly in terms of the president; (3) an event where an Iowa student had a chance to work with professionals while covering politics; and (4) considering scholastic reporters covering national candidates as they learn and…

  12. Covering Crime.

    ERIC Educational Resources Information Center

    Gest, Ted; Krajicek, David; Hackney, Suzette; Moore, Melissa

    2003-01-01

    Presents four brief articles on covering crime. Notes that reporting on crimes requires special skills for student reporters, editors, and photographers. Explains how to gain access to scenes, to develop journalistic ethics, and how to cover crime and its victims. Discusses the relation of race and ethnic issues to crime, and how visual…

  13. Luminosity and redshift dependence of the covering factor of active galactic nuclei viewed with WISE and Sloan digital sky survey

    SciTech Connect

    Toba, Y.; Matsuhara, H.; Oyabu, S.; Malkan, M. A.; Gandhi, P.; Nakagawa, T.; Isobe, N.; Shirahata, M.; Oi, N.; Takita, S.; Yano, K.; Ohyama, Y.; Yamauchi, C.

    2014-06-10

    In this work, we investigate the dependence of the covering factor (CF) of active galactic nuclei (AGNs) on the mid-infrared (MIR) luminosity and the redshift. We constructed 12 and 22 μm luminosity functions (LFs) at 0.006 ≤z ≤ 0.3 using Wide-field Infrared Survey Explorer (WISE) data. Combining the WISE catalog with Sloan Digital Sky Survey (SDSS) spectroscopic data, we selected 223,982 galaxies at 12 μm and 25,721 galaxies at 22 μm for spectroscopic classification. We then identified 16,355 AGNs at 12 μm and 4683 AGNs at 22 μm by their optical emission lines and cataloged classifications in the SDSS. Following that, we estimated the CF as the fraction of Type 2 AGN in all AGNs whose MIR emissions are dominated by the active nucleus (not their host galaxies) based on their MIR colors. We found that the CF decreased with increasing MIR luminosity, regardless of the choice of Type 2 AGN classification criteria, and the CF did not change significantly with redshift for z ≤ 0.2. Furthermore, we carried out various tests to determine the influence of selection bias and confirmed that similar dependences exist, even when taking these uncertainties into account. The luminosity dependence of the CF can be explained by the receding torus model, but the 'modified' receding torus model gives a slightly better fit, as suggested by Simpson.

  14. Luminosity and Redshift Dependence of the Covering Factor of Active Galactic Nuclei viewed with WISE and Sloan Digital Sky Survey

    NASA Astrophysics Data System (ADS)

    Toba, Y.; Oyabu, S.; Matsuhara, H.; Malkan, M. A.; Gandhi, P.; Nakagawa, T.; Isobe, N.; Shirahata, M.; Oi, N.; Ohyama, Y.; Takita, S.; Yamauchi, C.; Yano, K.

    2014-06-01

    In this work, we investigate the dependence of the covering factor (CF) of active galactic nuclei (AGNs) on the mid-infrared (MIR) luminosity and the redshift. We constructed 12 and 22 μm luminosity functions (LFs) at 0.006 <=z <= 0.3 using Wide-field Infrared Survey Explorer (WISE) data. Combining the WISE catalog with Sloan Digital Sky Survey (SDSS) spectroscopic data, we selected 223,982 galaxies at 12 μm and 25,721 galaxies at 22 μm for spectroscopic classification. We then identified 16,355 AGNs at 12 μm and 4683 AGNs at 22 μm by their optical emission lines and cataloged classifications in the SDSS. Following that, we estimated the CF as the fraction of Type 2 AGN in all AGNs whose MIR emissions are dominated by the active nucleus (not their host galaxies) based on their MIR colors. We found that the CF decreased with increasing MIR luminosity, regardless of the choice of Type 2 AGN classification criteria, and the CF did not change significantly with redshift for z <= 0.2. Furthermore, we carried out various tests to determine the influence of selection bias and confirmed that similar dependences exist, even when taking these uncertainties into account. The luminosity dependence of the CF can be explained by the receding torus model, but the "modified" receding torus model gives a slightly better fit, as suggested by Simpson.

  15. Effects of Leisure Education Programme Including Sportive Activities on Perceived Freedom in Leisure of Adolescents with Intellectual Disabilities

    ERIC Educational Resources Information Center

    Ertuzun, Ezgi

    2015-01-01

    The objective of this experimental study is to determine the effect of leisure education programme including sportive activities on the perceived freedom in leisure of adolescents with mild intellectual disabilities. The research was designed with an experimental group (n = 37) and a control group (n = 34), and was conducted among a total of 71…

  16. 25 CFR 170.623 - How are IRR Program projects and activities included in a self-governance agreement?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... other information required under 25 CFR 1000 subpart K. ... self-governance agreement? 170.623 Section 170.623 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE... self-governance agreement? To include an IRR Program project or activity in a self-governance...

  17. 25 CFR 170.623 - How are IRR Program projects and activities included in a self-governance agreement?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... other information required under 25 CFR 1000 subpart K. ... self-governance agreement? 170.623 Section 170.623 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE... self-governance agreement? To include an IRR Program project or activity in a self-governance...

  18. 25 CFR 170.623 - How are IRR Program projects and activities included in a self-governance agreement?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... other information required under 25 CFR 1000 subpart K. ... self-governance agreement? 170.623 Section 170.623 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE... self-governance agreement? To include an IRR Program project or activity in a self-governance...

  19. 25 CFR 170.623 - How are IRR Program projects and activities included in a self-governance agreement?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... other information required under 25 CFR 1000 subpart K. ... self-governance agreement? 170.623 Section 170.623 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE... self-governance agreement? To include an IRR Program project or activity in a self-governance...

  20. 25 CFR 170.623 - How are IRR Program projects and activities included in a self-governance agreement?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... other information required under 25 CFR 1000 subpart K. ... self-governance agreement? 170.623 Section 170.623 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE... self-governance agreement? To include an IRR Program project or activity in a self-governance...

  1. A lipid chemotactic factor from anaerobic coryneform bacteria including Corynebacterium parvum with activity for macrophages and monocytes.

    PubMed Central

    Russell, R J; McInroy, R J; Wilkinson, P C; White, R G

    1976-01-01

    A lipid with chemoattractant (chemotactic) activity for mouse and guinea-pig macrophages and for human blood monocytes is released by anaerobic coryneform bacteria (including Corynebacterium parvum). The active lipid is associated with fibrillar structures which lie on the outside of the bacterial cell and are released spontaneously during growth. The lipid can also be extracted easily by a number of methods. The fibrils are loosely associated with a capsule-like structure composed largely of polysaccharide. Purification of the active lipid was achieved by chloroform-methanol extraction of the whole organisms yielding a chloroform-soluble fraction attracting mononuclear phagocytes at concentrations around 10 microgram/ml. The infra-red spectrum of this material showed lipid but no peptide or sugar. Thin-layer chromatography yielded twelve spots of which three had chemoattractant properties. The most active of these gave staining reactions consistent with the presence of phospholipid, the other two probably contained free fatty acids and triglycerides. Thin-layer electrophoresis also yielded an active phosphorus-containing spot. Saturated fatty acids of chain lengths found in the anaerobic coryne forms had weak monocyte-attractant activity. As the active material was progressively purified, its activity as a monocyte attractant weakened. Images Figure 2 Figure 3 Figure 4 PMID:1027716

  2. MODIS Snow-Cover Products

    NASA Technical Reports Server (NTRS)

    Hall, Dorothy K.; Riggs, George A.; Salomonson, Vinvent V.; DiGirolamo, Nicolo; Bayr, Klaus J.; Houser, Paul (Technical Monitor)

    2001-01-01

    On December 18, 1999, the Terra satellite was launched with a complement of five instruments including the Moderate Resolution Imaging Spectroradiometer (MODIS). Many geophysical products are derived from MODIS data including global snow-cover products. These products have been available through the National Snow and Ice Data Center (NSIDC) Distributed Active Archive Center (DAAC) since September 13, 2000. MODIS snow-cover products represent potential improvement to the currently available operation products mainly because the MODIS products are global and 500-m resolution, and have the capability to separate most snow and clouds. Also the snow-mapping algorithms are automated which means that a consistent data set is generated for long-term climates studies that require snow-cover information. Extensive quality assurance (QA) information is stored with the product. The snow product suite starts with a 500-m resolution swath snow-cover map which is gridded to the Integerized Sinusoidal Grid to produce daily and eight-day composite tile products. The sequence then proceeds to a climate-modeling grid product at 5-km spatial resolution, with both daily and eight-day composite products. A case study from March 6, 2000, involving MODIS data and field and aircraft measurements, is presented. Near-term enhancements include daily snow albedo and fractional snow cover.

  3. Physiographic and land cover attributes of the Puget Lowland and the active streamflow gaging network, Puget Sound Basin

    USGS Publications Warehouse

    Konrad, Christopher; Sevier, Maria

    2014-01-01

    Geospatial information for the active streamflow gaging network in the Puget Sound Basin was compiled to support regional monitoring of stormwater effects to small streams. The compilation includes drainage area boundaries and physiographic and land use attributes that affect hydrologic processes. Three types of boundaries were used to tabulate attributes: Puget Sound Watershed Characterization analysis units (AU); the drainage area of active streamflow gages; and the catchments of Regional Stream Monitoring Program (RSMP) sites. The active streamflow gaging network generally includes sites that represent the ranges of attributes for lowland AUs, although there are few sites with low elevations (less than 60 meters), low precipitation (less than 1 meter year), or high stream density (greater than 5 kilometers per square kilometers). The active streamflow gaging network can serve to provide streamflow information in some AUs and RSMP sites, particularly where the streamflow gage measures streamflow generated from a part of the AU or that drains to the RSMP site, and that part of the AU or RSMP site is a significant fraction of the drainage area of the streamgage. The maximum fraction of each AU or RSMP catchment upstream of a streamflow gage and the maximum fraction of any one gaged basin in an AU or RSMP along with corresponding codes are provided in the attribute tables.

  4. Wall Covering

    NASA Technical Reports Server (NTRS)

    1978-01-01

    The attractive wall covering shown below is one of 132 styles in the Mirror Magic II line offered by The General Tire & Rubber Company, Akron, Ohio. The material is metallized plastic fabric, a spinoff from space programs. Wall coverings are one of many consumer applications of aluminized plastic film technology developed for NASA by a firm later bought by King-Seeley Thermos Company, Winchester, Massachusetts, which now produces the material. The original NASA use was in the Echo 1 passive communications satellite, a "space baloon" made of aluminized mylar; the high reflectivity of the metallized coating enabled relay of communications signals from one Earth station to another by "bouncing" them off the satellite. The reflectivity feature also made the material an extremely efficient insulator and it was subsequently widely used in the Apollo program for such purposes as temperature control of spacecraft components and insulation of tanks for fuels that must be maintained at very low temperatures. I Used as a wall covering, the aluminized material offers extra insulation, reflects light and I resists cracking. In addition to General Tire, King-Seeley also supplies wall covering material to Columbus Coated Fabrics Division of Borden, Incorporated, Columbus, Ohio, among others.

  5. Cover Crops

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cover crops are great tools to improve soil quality and health, and great tools to increase carbon sequestration. They are nutrient management tools that can help scavenge nitrate, cycle nitrogen to the following crop, mine NO3 from groundwater, and increase nitrogen use efficiency of cropping syste...

  6. Recent drought induced increase of non-photosynthetically active vegetation cover in the aspen forests of southern Rocky Mountain

    NASA Astrophysics Data System (ADS)

    Huang, C.; Anderegg, W.

    2011-12-01

    Severe droughts in concert with rising temperatures have triggered widespread of forest mortality across multiple tree species worldwide. Tree die-off would produce a significant amount of additional non-photosynthetically active vegetation (NPV), which is the major source of carbon (C) emissions to ecosystems. Trembling aspen (Populus tremuloides) is the most widely distributed tree species in North America and arguably among the largest known organisms in the world, reaching 6000 Mg in a single clone and storing a substantial amount of C in the system. A recent widespread aspen forest mortality (known as sudden aspen decline [SAD]) occurred in the last decade and its ramifications on C cycles of aspen forests and the impact on regional C budgets are not well known. Here we carry out a landscape scale assessment of NPV dynamics across 1186 km2 of aspen forests in southwestern Colorado, USA, which suffered some of the most severe forest biomass loss of the continent. We compared time-series (2000 [the pre-drought condition], 2002 [the driest period] and 2009 [the current condition]) projected NPV derived from summer Landsat Thematic Mapper (TM) images using an automated, probability based spectral mixture analysis model (AutoMCU) with aid from contemporary in-situ field observations conducted in 2009 and 2010. We found that SAD produced 40.3% more of NPV cover comparing to the pre-drought condition (mean ± standard deviation = 23.0 ± 15.8% in 2000 and 32.3 ± 19.0% in 2002) due to the senescence of top canopy aspen leaves that equated to additional 110.3 km2 of NPV cover increase in the region during the driest period. This NPV "ramp-up" also resulted in 22% decrease of green vegetation (mean ± standard deviation = 65.7 ± 18.0% in 2000 and 50.1 ± 18.8% in 2002) and 9.7% increase of visible albedo (3.7 ± 2.4% in 2000 and 4.1 ± 2.3% in 2002), which were also computed from TM images using AutoMCU and a Landsat-based albedo model, respectively. These rapid

  7. Trigonometry, Including Snell's Theorem.

    ERIC Educational Resources Information Center

    Kent, David

    1980-01-01

    Aspects of the instruction of trigonometry in secondary school mathematics are reviewed. Portions of this document cover basic introductions, a student-developed theorem, the cosine rule, inverse functions, and a sample outdoor activity. (MP)

  8. In Vitro Antimicrobial Activity of a Siderophore Cephalosporin, S-649266, against Enterobacteriaceae Clinical Isolates, Including Carbapenem-Resistant Strains.

    PubMed

    Kohira, Naoki; West, Joshua; Ito, Akinobu; Ito-Horiyama, Tsukasa; Nakamura, Rio; Sato, Takafumi; Rittenhouse, Stephen; Tsuji, Masakatsu; Yamano, Yoshinori

    2015-11-16

    S-649266 is a novel siderophore cephalosporin antibiotic with a catechol moiety on the 3-position side chain. Two sets of clinical isolate collections were used to evaluate the antimicrobial activity of S-649266 against Enterobacteriaceae. These sets included 617 global isolates collected between 2009 and 2011 and 233 β-lactamase-identified isolates, including 47 KPC-, 49 NDM-, 12 VIM-, and 8 IMP-producers. The MIC90 values of S-649266 against the first set of Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Citrobacter freundii, Enterobacter aerogenes, and Enterobacter cloacae isolates were all ≤1 μg/ml, and there were only 8 isolates (1.3%) among these 617 clinical isolates with MIC values of ≥8 μg/ml. In the second set, the MIC values of S-649266 were ≤4 μg/ml against 109 strains among 116 KPC-producing and class B (metallo) carbapenemase-producing strains. In addition, S-649266 showed MIC values of ≤2 μg/ml against each of the 13 strains that produced other types of carbapenemases such as SME, NMC, and OXA-48. The mechanisms of the decreased susceptibility of 7 class B carbapenemase-producing strains with MIC values of ≥16 μg/ml are uncertain. This is the first report to demonstrate that S-649266, a novel siderophore cephalosporin, has significant antimicrobial activity against Enterobacteriaceae, including strains that produce carbapenemases such as KPC and NDM-1.

  9. Activity of eravacycline against Enterobacteriaceae and Acinetobacter baumannii, including multidrug-resistant isolates, from New York City.

    PubMed

    Abdallah, Marie; Olafisoye, Olawole; Cortes, Christopher; Urban, Carl; Landman, David; Quale, John

    2015-03-01

    Eravacycline demonstrated in vitro activity against a contemporary collection of more than 4,000 Gram-negative pathogens from New York City hospitals, with MIC50/MIC90 values, respectively, for Escherichia coli of 0.12/0.5 μg/ml, Klebsiella pneumoniae of 0.25/1 μg/ml, Enterobacter aerogenes of 0.25/1 μg/ml, Enterobacter cloacae 0.5/1 μg/ml, and Acinetobacter baumannii of 0.5/1 μg/ml. Activity was retained against multidrug-resistant isolates, including those expressing KPC and OXA carbapenemases. For A. baumannii, eravacycline MICs correlated with increased expression of the adeB gene. PMID:25534744

  10. MODIS Snow-Cover Products

    NASA Technical Reports Server (NTRS)

    Hall, Dorothy K.; Riggs, George A.; Salomonson, Vincent V.; DiGirolamo, Nicole E.; Bayr, Klaus J.; Houser, Paul R. (Technical Monitor)

    2002-01-01

    On December 18, 1999, the Terra satellite was launched with a complement of five instruments including the Moderate Resolution Imaging Spectroradiometer (MODIS). Many geophysical products are derived from MODIS data including global snow-cover products. MODIS snow and ice products have been available through the National Snow and Ice Data Center (NSIDC) Distributed Active Archive Center (DAAC) since September 13, 2000. MODIS snow-cover products represent potential improvement to or enhancement of the currently-available operational products mainly because the MODIS products are global and 500-m resolution, and have the capability to separate most snow and clouds. Also the snow-mapping algorithms are automated which means that a consistent data set may be generated for long-term climate studies that require snow-cover information. Extensive quality assurance (QA) information is stored with the products. The MODIS snow product suite begins with a 500-m resolution, 2330-km swath snow-cover map which is then gridded to an integerized sinusoidal grid to produce daily and 8-day composite tile products. The sequence proceeds to a climate-modeling grid (CMG) product at about 5.6-km spatial resolution, with both daily and 8-day composite products. Each pixel of the CMG contains fraction of snow cover from 40 - 100%. Measured errors of commission in the CMG are low, for example, on the continent of Australia in the spring, they vary from 0.02 - 0.10%. Near-term enhancements include daily snow albedo and fractional snow cover. A case study from March 6, 2000, involving MODIS data and field and aircraft measurements, is presented to show some early validation work.

  11. An Analysis of the Learning Activities Covered in the 5th Grade Science Textbooks Based on 2005 and 2013 Turkish Science Curricula

    ERIC Educational Resources Information Center

    Aydogdu, Cemil; Idin, Sahin

    2015-01-01

    The aim of this study is to analyze the learning activities covered in 5th grade elementary science textbooks which depend on 2005 and 2013 elementary science curricula. Two elementary science textbooks [which] depend on 2005 science curriculum and two elementary science textbooks [which] depend on 2013 science curriculum were researched. The…

  12. An Analysis of the Learning Activities Covered in the 5th Grade Science Textbooks Based on 2005 and 2013 Turkish Science Curricula

    ERIC Educational Resources Information Center

    Aydogdu, Cemil; Idin, Sahin

    2015-01-01

    The aim of this study is to analyze the learning activities covered in 5th grade elementary science textbooks which depend on 2005 and 2013 elementary science curricula. Two elementary science textbooks depends on 2005 science curriculum and two elementary science textbooks depend on 2013 science curriculum were researched. The study is a…

  13. 43 CFR 2884.25 - What activities may I conduct on BLM lands covered by my application for a grant or TUP while BLM...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false What activities may I conduct on BLM lands covered by my application for a grant or TUP while BLM is processing my application? 2884.25 Section 2884.25 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR LAND...

  14. 43 CFR 2804.29 - What activities may I conduct on the lands covered by the proposed right-of-way while BLM is...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false What activities may I conduct on the lands covered by the proposed right-of-way while BLM is processing my application? 2804.29 Section 2804.29 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR LAND...

  15. The NLP toxin family in Phytophthora sojae includes rapidly evolving groups that lack necrosis-inducing activity.

    PubMed

    Dong, Suomeng; Kong, Guanghui; Qutob, Dinah; Yu, Xiaoli; Tang, Junli; Kang, Jixiong; Dai, Tingting; Wang, Hai; Gijzen, Mark; Wang, Yuanchao

    2012-07-01

    Necrosis- and ethylene-inducing-like proteins (NLP) are widely distributed in eukaryotic and prokaryotic plant pathogens and are considered to be important virulence factors. We identified, in total, 70 potential Phytophthora sojae NLP genes but 37 were designated as pseudogenes. Sequence alignment of the remaining 33 NLP delineated six groups. Three of these groups include proteins with an intact heptapeptide (Gly-His-Arg-His-Asp-Trp-Glu) motif, which is important for necrosis-inducing activity, whereas the motif is not conserved in the other groups. In total, 19 representative NLP genes were assessed for necrosis-inducing activity by heterologous expression in Nicotiana benthamiana. Surprisingly, only eight genes triggered cell death. The expression of the NLP genes in P. sojae was examined, distinguishing 20 expressed and 13 nonexpressed NLP genes. Real-time reverse-transcriptase polymerase chain reaction results indicate that most NLP are highly expressed during cyst germination and infection stages. Amino acid substitution ratios (Ka/Ks) of 33 NLP sequences from four different P. sojae strains resulted in identification of positive selection sites in a distinct NLP group. Overall, our study indicates that expansion and pseudogenization of the P. sojae NLP family results from an ongoing birth-and-death process, and that varying patterns of expression, necrosis-inducing activity, and positive selection suggest that NLP have diversified in function.

  16. 45 CFR 90.3 - What programs or activities does the Age Discrimination Act of 1975 cover?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Discrimination Act of 1975 cover? 90.3 Section 90.3 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES...) An age distinction contained in that part of a Federal, State or local statute or ordinance adopted by an elected, general purpose legislative body which: (i) Provides any benefits or assistance...

  17. WATER COOLED RETORT COVER

    DOEpatents

    Ash, W.J.; Pozzi, J.F.

    1962-05-01

    A retort cover is designed for use in the production of magnesium metal by the condensation of vaporized metal on a collecting surface. The cover includes a condensing surface, insulating means adjacent to the condensing surface, ind a water-cooled means for the insulating means. The irrangement of insulation and the cooling means permits the magnesium to be condensed at a high temperature and in massive nonpyrophoric form. (AEC)

  18. Validation of a high throughput flow cytometric in vitro micronucleus assay including assessment of metabolic activation in TK6 cells.

    PubMed

    Thougaard, Annemette V; Christiansen, Joan; Mow, Tomas; Hornberg, Jorrit J

    2014-12-01

    Genotoxicity is an unacceptable property for new drug candidates and we employ three screening assays during the drug discovery process to identify genotoxicity early and optimize chemical series. One of these methods is the flow cytometric in vitro micronucleus assay for which protocol optimizations have been described recently. Here, we report further validation of the assay in TK6 cells including assessment of metabolic activation. We first optimized assay conditions to allow for testing with and without metabolic activation in parallel in a 96-well plate format. Then, we tested a set of 48 compounds carefully selected to contain known in vivo genotoxins, nongenotoxins and drugs. Avoidance of irrelevant positives, a known issue with mammalian cell-based genotoxicity assays, is important to prevent early deselection of potentially promising compounds. Therefore, we enriched the validation set with compounds that were previously reported to produce irrelevant positive results in mammalian cell-based genotoxicity assays. The resulting dataset was used to set the relevant cut-off values for scoring a compound positive or negative, such that we obtained an optimal balance of high sensitivity (88%) and high specificity (87%). Finally, we tested an additional set of 16 drugs to further probe assay performance and 14 of them were classified correctly. To our knowledge, the present study is the most comprehensive validation of the in vitro flow cytometric micronucleus assay and the first to report parallel assessment with metabolic activation in reasonable throughput. The assay allows for rapidly screening novel compounds for genotoxicity and is therefore well-suited for use in early drug discovery projects. Environ.

  19. Cover Picture.

    PubMed

    Breuning; Ruben; Lehn; Renz; Garcia; Ksenofontov; Gütlich; Wegelius; Rissanen

    2000-07-17

    The cover picture shows how both, fine arts and science, avail themselves of a system of intertwined symbolic and iconic languages. They make use of a common set of abstracted signs to report on their results. Thus, already in 1925, Wassily Kandinsky painted a masterpiece (bottom), which now, 75 years later, might be regarded as a blueprint for a scientific project. In his painting, Kandinsky pictured a grid-shaped sign that resembles in effect an actual molecular switch. Apparently following an enigmatic protocol, the groups of Lehn and Gütlich (see p. 2504 ff. for more details) constructed a grid-type inorganic architecture that operates as a three-level magnetic switch (center) triggered by three external perturbations (p, T, hnu). The switching principle is based on the spin-crossover phenomenon of Fe(II) ions and can be monitored by Mössbauer spectroscopy (left) and magnetic measurements (rear). Maybe not by chance, the English translation of the title of the painting "signs" is a homonym of "science", since both presented works are a product of the insatiable curiosity of man and his untiring desire to recognize his existence.

  20. Reclassified Cropland Active Fire and Burned Area Detections by the MODIS 1 km Sensor in Canadian Provinces by land cover type, 2001 - 2010

    NASA Astrophysics Data System (ADS)

    Kerr, T. F.; Ernst, C. L.; McCarty, J. L.

    2011-12-01

    Fire is a primary disturbance agent in Canadian ecosystems and has significant social, environmental, and economic consequences. Accurate location and identification of biomass burning is critical to understanding the transfer of gases and particles into earth's atmosphere, especially in Northern latitudes. This data is an important aid in producing accurate atmospheric models that estimate black carbon (BC) deposition on arctic snow. Previous research has indicated that cropland burning contributes to BC distribution in the arctic which alters the balance in snow-albedo reflectance and radiation transmission in the atmosphere. The locations and numbers of fires were identified using the 1km MODIS Active Fire Product and the 500m MODIS Burned Area Product. Land cover type was assigned based on the 1 km MODIS Land Cover Product, to the post-processed active fire points. They were then reclassified into seven (7) classes: Croplands, Forest, Grasslands, Urban, Water Bodies, Wetlands, and Barren. The results show that Forest, Cropland, and Grassland land cover types are the main sources of active fire detections in Canada from 2001 to 2010. The peak fire months are April, May, September, and October for Cropland active fire burns in all Canadian Provinces from 2001 to 2010. By province, Saskatchewan and Manitoba are the leading sources of Cropland detected active fires. Cropland burned area estimations were calculated using the burned area pixel count (post-processing of MODIS Burned Area Product) within cropland identified by the 1 km MODIS Land Cover data set (LC-12) for the years 2003-2010. Cropland burned area detection was most significant in 2003 during which 27.3% of all detected hectares burned from 2003 to 2010 occurred. The year with least impact was 2004 in which 3.5% of all detected hectares burned. The peak months for Cropland burned area detections were May, September, and October across all Canadian Provinces from 2003 to 2010. Saskatchewan, Manitoba

  1. Evapotranspiration (ET) covers.

    PubMed

    Rock, Steve; Myers, Bill; Fiedler, Linda

    2012-01-01

    Evapotranspiration (ET) cover systems are increasingly being used at municipal solid waste (MSW) landfills, hazardous waste landfills, at industrial monofills, and at mine sites. Conventional cover systems use materials with low hydraulic permeability (barrier layers) to minimize the downward migration of water from the surface to the waste (percolation), ET cover systems use water balance components to minimize percolation. These cover systems rely on soil to capture and store precipitation until it is either transpired through vegetation or evaporated from the soil surface. Compared to conventional membrane or compacted clay cover systems, ET cover systems are expected to cost less to construct. They are often aesthetic because they employ naturalized vegetation, require less maintenance once the vegetative system is established, including eliminating mowing, and may require fewer repairs than a barrier system. All cover systems should consider the goals of the cover in terms of protectiveness, including the pathways of risk from contained material, the lifecycle of the containment system. The containment system needs to be protective of direct contact of people and animals with the waste, prevent surface and groundwater water pollution, and minimize release of airborne contaminants. While most containment strategies have been based on the dry tomb strategy of keeping waste dry, there are some sites where adding or allowing moisture to help decompose organic waste is the current plan. ET covers may work well in places where complete exclusion of precipitation is not needed. The U.S. EPA Alternative Cover Assessment Program (ACAP), USDOE, the Nuclear Regulatory Commission, and others have researched ET cover design and efficacy, including the history of their use, general considerations in their design, performance, monitoring, cost, current status, limitations on their use, and project specific examples. An on-line database has been developed with information

  2. Reusable pipe flange covers

    DOEpatents

    Holden, James Elliott; Perez, Julieta

    2001-01-01

    A molded, flexible pipe flange cover for temporarily covering a pipe flange and a pipe opening includes a substantially round center portion having a peripheral skirt portion depending from the center portion, the center portion adapted to engage a front side of the pipe flange and to seal the pipe opening. The peripheral skirt portion is formed to include a plurality of circumferentially spaced tabs, wherein free ends of the flexible tabs are formed with respective through passages adapted to receive a drawstring for pulling the tabs together on a back side of the pipe flange.

  3. [Evaluation of an obesity prevention intervention which included nutrition education and physical activity applied in public schools of Santiago, Chile].

    PubMed

    Kain, Juliana; Leyton, Bárbara; Concha, Fernando; Weisstaub, Gerardo; Lobos, Luz; Bustos, Nelly; Vio, Fernando

    2012-03-01

    The main objective of this study was to assess the effectiveness of an obesity prevention intervention which included nutrition education and physical activity applied to low income Chilean children in a longitudinal three-year follow-up study. Participants included all 4-7 year old children from 7 public schools located in a low socioeconomic district of Santiago. The main aspects of the intervention were: training of teachers so they could apply an educational program on healthy eating, increase in physical education classes from 3 to 4 per week, and improvement of their quality. Weight, height, waist circumference (WC) and the 6-minute walk distance (6MWD) were assessed yearly in 597 children. We calculated BMI, BMI Z, % normal (N), overweight (OW) and obesity (OB), WC > 90th percentile (NHANES III) and fitness (6MWD/height). Annual changes in BMI Z, WC, 6MWD and fitness were assessed, using repeated measures ANOVA and the test of proportions. Knowledge in healthy eating was assessed during 2 of the 3 years. Results showed that % OB remained unchanged for 2 years (17%) but increased to 19.3% at follow-up. BMI Z increased from 0.3 to 0.38 (p = 0.052) in the N, remained unchanged in the OW, while decreasing significantly in the OB (2.73 to 2.41 p < 0.0001). % WC > 90 th percentile decreased in the OW and OB; 6 MWD was higher in the OW, but the increase in distance overtime was greater among the N. Overall fitness improved, however it was only significant among the N (p = 0.0002). There was a significant increase in food knowledge. We conclude that in spite of a decrease in BMI Z of the OB, an improvement in fitness in the N and food knowledge in all the children, obesity increased at follow-up. This study shows that in school-based obesity programs it is necessary to apply more intense interventions with another type of methodology; otherwise this condition will continue to rise.

  4. Fibrinogenolytic and anticoagulant activities in the tissue covering the stingers of marine stingrays Dasyatis sephen and Aetobatis narinari.

    PubMed

    Kumar, Kalainesan Rajesh; Vennila, Rathinam; Kanchana, Shankar; Arumugam, Muthuvel; Balasubramaniam, Thangavel

    2011-05-01

    Stingray envenomation is one of the major problems in the marine and freshwater ecosystem. Accidents in human cause immediate, local and intense pain, erythema, edema, hemorrhage, tissue necrosis and secondary bacterial infection are also common. To determine the effect of two marine stingray species Dasyatis sephen and Aetobatis narinari venom extract on coagulation, fibrin(ogen)olytic, proteolytic activities. Plasma coagulation, Thrombin catalyzed fibrinocoagulation, Fibrin plate assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), substrate SDS-PAGE and thrombin like activity by using chromogenic substrate were used to determine the effect of venom on plasma coagulation, its fibrin(ogen)olytic and proteolytic activity. The results show the presence of fibrin(ogen)olytic, anticoagulant and gelatinolytic activity in both stingray venom extracts. D. sephen venom delays coagulation of citrated plasma more significantly than A. narinari upon using increasing concentration of the venom. The same results were obtained in the fibrinocoagulation assays. SDS-PAGE analysis of fibrinogen and fibrin after incubation with D. sephen and A. narinari venom show fibrin(ogen)olytic activity. Through SDS-PAGE analysis it is confirmed that the delaying in coagulation process by stingray venom is due to its fibrin(ogen)olytic activity and fibrinolytic activity also confirmed through fibrin plate assay. Zymogram analysis shows the presence of array of gelatinolytic and fibrinogenolytic enzymes above 43-276 kDa in the D. sephen and A. narinari venom respectively. Protease inhibitor studies show the serine and metallo proteases are responsible for these activities. From the results, fibrinogenolytic, proteolytic activity of the stingray venom is confirmed, but it has no thrombin like activity and these activities may aid in hemorrhages, tissue necrosis and secondary bacterial infections at the site of envenomation. PMID:21165674

  5. 25 CFR 170.137 - What types of activities can a recreation, tourism, and trails program include?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false What types of activities can a recreation, tourism, and... Eligibility Recreation, Tourism and Trails § 170.137 What types of activities can a recreation, tourism, and... may perform under a recreation, tourism, and trails program: (1) Transportation planning for...

  6. 25 CFR 170.137 - What types of activities can a recreation, tourism, and trails program include?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 1 2012-04-01 2011-04-01 true What types of activities can a recreation, tourism, and... Eligibility Recreation, Tourism and Trails § 170.137 What types of activities can a recreation, tourism, and... may perform under a recreation, tourism, and trails program: (1) Transportation planning for...

  7. 25 CFR 170.137 - What types of activities can a recreation, tourism, and trails program include?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false What types of activities can a recreation, tourism, and... Eligibility Recreation, Tourism and Trails § 170.137 What types of activities can a recreation, tourism, and... may perform under a recreation, tourism, and trails program: (1) Transportation planning for...

  8. 25 CFR 170.137 - What types of activities can a recreation, tourism, and trails program include?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false What types of activities can a recreation, tourism, and... Eligibility Recreation, Tourism and Trails § 170.137 What types of activities can a recreation, tourism, and... may perform under a recreation, tourism, and trails program: (1) Transportation planning for...

  9. 25 CFR 170.137 - What types of activities can a recreation, tourism, and trails program include?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false What types of activities can a recreation, tourism, and... Eligibility Recreation, Tourism and Trails § 170.137 What types of activities can a recreation, tourism, and... may perform under a recreation, tourism, and trails program: (1) Transportation planning for...

  10. HEPS Inventory Tool: An Inventory Tool Including Quality Assessment of School Interventions on Healthy Eating and Physical Activity

    ERIC Educational Resources Information Center

    Dadaczynski, Kevin; Paulus, Peter; de Vries, Nanne; de Ruiter, Silvia; Buijs, Goof

    2010-01-01

    The HEPS Inventory Tool aims to support stakeholders working in school health promotion to promote high quality interventions on healthy eating and physical activity. As a tool it provides a step-by-step approach on how to develop a national or regional inventory of existing school based interventions on healthy eating and physical activity. It…

  11. ESTIMATING IMPERVIOUS COVER FROM REGIONALLY AVAILABLE DATA

    EPA Science Inventory

    The objective of this study is to compare and evaluate the reliability of different approaches for estimating impervious cover including three empirical formulations for estimating impervious cover from population density data, estimation from categorized land cover data, and to ...

  12. Effects of restricted feeding on daily fluctuations of hepatic functions including p450 monooxygenase activities in rats.

    PubMed

    Hirao, Jun; Arakawa, Shingo; Watanabe, Kyoko; Ito, Kazumi; Furukawa, Tadashi

    2006-02-10

    Hepatic P450 monooxygenase activities, assessed by measurement of 7-alkoxycoumarin O-dealkylase (ACD) activities, show obvious daily fluctuations in male rats with high values during the dark period and low values during the light period. We have already confirmed that the ACD activities are controlled by the suprachiasmatic nucleus (SCN), which is well known as the oscillator of circadian rhythm. Recently, it is reported that circadian oscillators exist not only in the SCN but also in peripheral organs. To date, it is unclear which circadian oscillators predominantly drive the daily fluctuations of hepatic ACD activities. To address this question, we examined the effects of restricted feeding, which uncouples the circadian oscillators in the liver from the central pacemaker in the SCN, on the daily fluctuations in hepatic ACD activities in male rats. Here we show that restricted feeding inverts the oscillation phase of the daily fluctuations in hepatic ACD activities. Regarding the hepatic P450 content, there were no fluctuations between the light and dark periods under ad libitum and restricted feeding conditions. Therefore, it is considered that the daily fluctuations in hepatic ACD activities are predominantly driven by the circadian factors in peripheral organs rather than by the oscillator in the SCN directly.

  13. Simple fabrication of hydrophilic nanochannels using the chemical bonding between activated ultrathin PDMS layer and cover glass by oxygen plasma.

    PubMed

    Kim, So Hyun; Cui, Yidan; Lee, Min Jung; Nam, Seong-Won; Oh, Doori; Kang, Seong Ho; Kim, Youn Sang; Park, Sungsu

    2011-01-21

    This study describes a simple and low cost method for fabricating enclosed transparent hydrophilic nanochannels by coating low-viscosity PDMS (monoglycidyl ether-terminated polydimethylsiloxane) as an adhesion layer onto the surface of the nanotrenches that are molded with a urethane-based UV-curable polymer, Norland Optical Adhesive (NOA 63). In detail, the nanotrenches made of NOA 63 were replicated from a Si master mold and coated with 6 nm thick layer of PDMS. These nanotrenches underwent an oxygen plasma treatment and finally were bound to a cover glass by chemical bonding between silanol and hydroxyl groups. Hydrophobic recovery that is observed in the bulk PDMS was not observed in the thin film of PDMS on the mold and the PDMS-coated nanochannel maintained its surface hydrophilicity for at least one month. The potentials of the nanochannels for bioapplications were demonstrated by stretching λ-DNA (48,502 bp) in the channels. Therefore, this fabrication approach provides a practical solution for the simple fabrication of the nanochannels for bioapplications.

  14. Antiviral activity of human Vδ2 T-cells against WNV includes both cytolytic and non-cytolytic mechanisms.

    PubMed

    Agrati, Chiara; Castilletti, Concetta; Cimini, Eleonora; Romanelli, Antonella; Lapa, Daniele; Quartu, Serena; Martini, Federico; Capobianchi, Maria Rosaria

    2016-04-01

    West Nile virus (WNV) causes a severe central nervous system infection in humans, primarily in the elderly and immunocompromised subjects. Human γδ T-cells play a critical role in the immune response against viruses, and studies of WNV meningoencephalitis in laboratory mice described a role of γδ T-cells in the protective immune response. Aim of this study was to analyze the cytolytic and non-cytolytic antiviral activity of human Vδ2 T-cells against WNV replication. The anti-WNV activity of soluble factor released by zoledronic acid (ZA)-activated Vδ2 T-cell lines and the cytotoxic capability of Vδ2 T-cell lines against WNV-infected cells were tested in vitro. The activation of Vδ2 T-cell lines was able to inhibit WNV replication through the release of soluble factors. IFN-γ is massively released by activated Vδ2 T-cell lines and is involved in the anti-WNV activity. Moreover, the Vδ2 T-cell lines can efficiently kill WNV-infected cells possibly through perforin-mediated mechanism. Altogether, our results provide insight into the effector functions of human Vδ2 T-cells against WNV. The possibility to target these cells by ZA, a commercially available drug used in humans, could potentially offer a new immunotherapeutic strategy for WNV infection. PMID:27196553

  15. Effects of Land-Cover Change, Floods, and Stream Position on Geomorphic Processes - Implications for Restoration Activities

    USGS Publications Warehouse

    Fitzpatrick, F.A.; ,

    2001-01-01

    A geomorphic study for North Fish Creek, a northern Wisconsin tributary to Lake Superior was analyzed to determine the hydrologic and geomorphic changes caused by clear-cut logging and agricultural activity. Discharge magnitude estimated with HEC-2 for full-channel capacities indicate that modern full-channel discharges are about twice as large as pre-1946 full-channel discharges. Flood-plain deposition rates were high along the transitional main stem after European settlement. Restoration and protection activities would be most effective if focused on watershed practices to reduce runoff and on channel restoration that reduce buff and bank erosion in the upper and transitional main stems.

  16. My House Is Covered with Papers! Reflections on a Generation of Active Citizenship. Community Supported Living Series.

    ERIC Educational Resources Information Center

    O'Brien, Connie Lyle; O'Brien, John

    This booklet highlights some of the insights that five mothers of children with developmental disabilities have gained after a generation of working together to improve the lives of people with developmental disabilities in Wisconsin. It discusses civic activism, the critical importance of organized parent support, difficulties in collaborating…

  17. Age Discrimination in Employment Act of 1967; A Report Covering Activities in Connection with the Act During 1969.

    ERIC Educational Resources Information Center

    Wage and Labor Standards Administration (DOL), Washington, DC.

    This annual report on the Age Discrimination in Employment Act describes activities conducted under the Act in 1969. The Act prohibits discrimination in any of the terms of employment against individuals between 40 and 65 years of age. Coverage is extended to employers of 25 or more persons in occupations for which age is not a bona fide…

  18. Spatial Heterogeneity of Ice Cover Sediment and Thickness and Its Effects on Photosynthetically Active Radiation and Chlorophyll-a Distribution: Lake Bonney, Antarctica

    NASA Astrophysics Data System (ADS)

    Obryk, M.; Doran, P. T.; Priscu, J. C.; Morgan-Kiss, R. M.; Siebenaler, A. G.

    2012-12-01

    The perennially ice-covered lakes in the McMurdo Dry Valleys, Antarctica have been extensively studied under the Long Term Ecological Research project. But sampling has been spatially restricted due to the logistical difficulty of penetrating the 3-6 m of ice cover. The ice covers restrict wind-driven turbulence and its associated mixing of water, resulting in a unique thermal stratification and a strong vertical gradient of salinity. The permanent ice covers also shade the underlying water column, which, in turn, controls photosynthesis. Here, we present results of a three-dimensional record of lake processes obtained with an autonomous underwater vehicle (AUV). The AUV was deployed at West Lake Bonney, located in Taylor Valley, Dry Valleys, to further understand biogeochemical and physical properties of the Dry Valley lakes. The AUV was equipped with depth, conductivity, temperature, under water photosynthetically active radiation (PAR), turbidity, chlorophyll-and-DOM fluorescence, pH, and REDOX sensors. Measurements were taken over the course of two years in a 100 x 100 meter spaced horizontal sampling grid (and 0.2 m vertical resolution). In addition, the AUV measured ice thickness and collected 200 images looking up through the ice, which were used to quantify sediment distribution. Comparison with high-resolution satellite QuickBird imagery demonstrates a strong correlation between aerial sediment distribution and ice cover thickness. Our results are the first to show the spatial heterogeneity of lacustrine ecosystems in the McMurdo Dry Valleys, significantly improving our understanding of lake processes. Surface sediment is responsible for localized thinning of ice cover due to absorption of solar radiation, which in turn increases total available PAR in the water column. Higher PAR values are negatively correlated with chlorophyll-a, presenting a paradox; historically, long-term studies of PAR and chlorophyll-a have shown positive trends. We hypothesized

  19. 14 CFR 440.11 - Duration of coverage for licensed launch, including suborbital launch, or permitted activities...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... LICENSING FINANCIAL RESPONSIBILITY Financial Responsibility for Licensed and Permitted Activities § 440.11...; modifications. (a) Insurance coverage required under § 440.9, or other form of financial responsibility, shall... recovery; or (ii) The FAA's determination that risk to third parties and Government property as a result...

  20. Activated Notch1 Target Genes during Embryonic Cell Differentiation Depend on the Cellular Context and Include Lineage Determinants and Inhibitors

    PubMed Central

    Meier-Stiegen, Franziska; Schwanbeck, Ralf; Bernoth, Kristina; Martini, Simone; Hieronymus, Thomas; Ruau, David; Zenke, Martin; Just, Ursula

    2010-01-01

    Background Notch receptor signaling controls developmental cell fates in a cell-context dependent manner. Although Notch signaling directly regulates transcription via the RBP-J/CSL DNA binding protein, little is known about the target genes that are directly activated by Notch in the respective tissues. Methodology/Principal Findings To analyze how Notch signaling mediates its context dependent function(s), we utilized a Tamoxifen-inducible system to activate Notch1 in murine embryonic stem cells at different stages of mesodermal differentiation and performed global transcriptional analyses. We find that the majority of genes regulated by Notch1 are unique for the cell type and vary widely dependent on other signals. We further show that Notch1 signaling regulates expression of genes playing key roles in cell differentiation, cell cycle control and apoptosis in a context dependent manner. In addition to the known Notch1 targets of the Hes and Hey families of transcriptional repressors, Notch1 activates the expression of regulatory transcription factors such as Sox9, Pax6, Runx1, Myf5 and Id proteins that are critically involved in lineage decisions in the absence of protein synthesis. Conclusion/Significance We suggest that Notch signaling determines lineage decisions and expansion of stem cells by directly activating both key lineage specific transcription factors and their repressors (Id and Hes/Hey proteins) and propose a model by which Notch signaling regulates cell fate commitment and self renewal in dependence of the intrinsic and extrinsic cellular context. PMID:20628604

  1. Weight management including dietary and physical activity advice provided by Australian physiotherapists: a pilot cross-sectional survey.

    PubMed

    Snodgrass, Suzanne J; Carter, Amy E; Guest, Maya; Collins, Clare E; James, Carole; Kable, Ashley K; Ashby, Samantha E; Plotnikoff, Ronald C

    2014-08-01

    Physiotherapists may have an impact on obesity prevention and treatment by providing nutrition and physical activity advice to overweight or obese clients; yet little is known about physiotherapists' beliefs and practices related to client weight management. The aim of this pilot study was to determine the practices, beliefs, attitudes and knowledge of physiotherapists regarding the provision of weight management advice to overweight or obese clients. Physiotherapists (n = 65) working in multiple practice settings completed a self-administered questionnaire. Logistic regression determined factors associated with the provision of dietary and physical activity advice for weight management. The majority of physiotherapists (n = 53 [81.5%]) believed providing weight management advice was within their scope of practice, yet only a minority had received training during their professional entry level education (n = 13 [20%]) or through professional development (n = 7 [11%]). Most physiotherapists (n = 55 [84.6%]) provided physical activity advice for weight management, but a minority (n = 27 [41.5%]) provided dietary advice. Having received training in weight management during their professional entry level education was associated with providing dietary advice (Odds ratio 8.8, 95% confidence interval 2.0-38.9, p = 0.004). Training in weight management may increase the likelihood of physiotherapists providing dietary advice, improving physiotherapists' management of obesity.

  2. The MRX Complex Ensures NHEJ Fidelity through Multiple Pathways Including Xrs2-FHA–Dependent Tel1 Activation

    PubMed Central

    Iwasaki, Daichi; Hayashihara, Kayoko; Shima, Hiroki; Higashide, Mika; Terasawa, Masahiro; Gasser, Susan M.; Shinohara, Miki

    2016-01-01

    Because DNA double-strand breaks (DSBs) are one of the most cytotoxic DNA lesions and often cause genomic instability, precise repair of DSBs is vital for the maintenance of genomic stability. Xrs2/Nbs1 is a multi-functional regulatory subunit of the Mre11-Rad50-Xrs2/Nbs1 (MRX/N) complex, and its function is critical for the primary step of DSB repair, whether by homologous recombination (HR) or non-homologous end joining. In human NBS1, mutations result truncation of the N-terminus region, which contains a forkhead-associated (FHA) domain, cause Nijmegen breakage syndrome. Here we show that the Xrs2 FHA domain of budding yeast is required both to suppress the imprecise repair of DSBs and to promote the robust activation of Tel1 in the DNA damage response pathway. The role of the Xrs2 FHA domain in Tel1 activation was independent of the Tel1-binding activity of the Xrs2 C terminus, which mediates Tel1 recruitment to DSB ends. Both the Xrs2 FHA domain and Tel1 were required for the timely removal of the Ku complex from DSB ends, which correlates with a reduced frequency of imprecise end-joining. Thus, the Xrs2 FHA domain and Tel1 kinase work in a coordinated manner to maintain DSB repair fidelity. PMID:26990569

  3. The IKAROS Interaction with a Complex Including Chromatin Remodeling and Transcription Elongation Activities Is Required for Hematopoiesis

    PubMed Central

    Bottardi, Stefania; Mavoungou, Lionel; Pak, Helen; Daou, Salima; Bourgoin, Vincent; Lakehal, Yahia A.; Affar, El Bachir; Milot, Eric

    2014-01-01

    IKAROS is a critical regulator of hematopoietic cell fate and its dynamic expression pattern is required for proper hematopoiesis. In collaboration with the Nucleosome Remodeling and Deacetylase (NuRD) complex, it promotes gene repression and activation. It remains to be clarified how IKAROS can support transcription activation while being associated with the HDAC-containing complex NuRD. IKAROS also binds to the Positive-Transcription Elongation Factor b (P-TEFb) at gene promoters. Here, we demonstrate that NuRD and P-TEFb are assembled in a complex that can be recruited to specific genes by IKAROS. The expression level of IKAROS influences the recruitment of the NuRD-P-TEFb complex to gene regulatory regions and facilitates transcription elongation by transferring the Protein Phosphatase 1α (PP1α), an IKAROS-binding protein and P-TEFb activator, to CDK9. We show that an IKAROS mutant that is unable to bind PP1α cannot sustain gene expression and impedes normal differentiation of IkNULL hematopoietic progenitors. Finally, the knock-down of the NuRD subunit Mi2 reveals that the occupancy of the NuRD complex at transcribed regions of genes favors the relief of POL II promoter-proximal pausing and thereby, promotes transcription elongation. PMID:25474253

  4. Simultaneous BVI noise and vibration reduction in rotorcraft using actively-controlled flaps and including performance considerations

    NASA Astrophysics Data System (ADS)

    Patt, Daniel A.

    This work presents the development and application of an active control approach for reduction of both vibration and noise induced by helicopter rotor blade vortex interaction (BVI). Control is implemented through single or dual actively controlled flaps (ACFs) on each blade. Low-speed helicopter flight is prone to severe BVI, resulting in elevated vibration and noise levels. Existing research has suggested that when some form of active control is used to reduce vibration, noise will increase and vice versa. The present research achieves simultaneous reduction of noise and vibration, and also investigates the physical sources of the observed reduction. The initial portion of this work focused on developing a tool for simulating helicopter noise and vibrations in the BVI flight regime. A method for predicting compressible unsteady blade surface pressure distribution on rotor blades was developed and combined with an enhanced free-wake model and an acoustic prediction tool with provisions for blade flexibility. These elements were incorporated within an aeroelastic analysis featuring fully coupled flap-lag-torsional blade dynamics. Subsequently, control algorithms were developed that were effective for reducing noise and vibration even in the nonlinear BVI flight regime; saturation limits were incorporated constraining flap deflections to specified limits. The resulting simulation was also validated with a wide range of experimental data, achieving excellent correlation. Finally, a number of active control studies were performed. Multi-component vibration reductions of 40--80% could be achieved, while incurring a small noise penalty. Noise was reduced using an onboard feedback microphone; reductions of 4--10 dB on the advancing side were observed on a plane beneath the rotor when using dual flaps. Finally, simultaneous noise and vibration reduction was studied. A reduction of about 5 dB in noise on the advancing side combined with a 60% reduction in vibration was

  5. Crop cover the principal influence on non-crop ground beetle (Coleoptera, Carabidae) activity and assemblages at the farm scale in a long-term assessment.

    PubMed

    Eyre, M D; Sanderson, R A; McMillan, S D; Critchley, C N R

    2016-04-01

    Ground beetle data were generated using pitfall traps in the 17-year period from 1993 to 2009 and used to investigate the effects of changes in surrounding crop cover on beetle activity and assemblages, together with the effects of weather variability. Beetles were recorded from non-crop field margins (overgrown hedges). Crop cover changes explained far more variation in the beetle assemblages recorded than did temperature and rainfall variation. A reduction in management intensity and disturbance in the crops surrounding the traps, especially the introduction and development of willow coppice, was concomitant with changes in individual species activity and assemblage composition of beetles trapped in non-crop habitat. There were no consistent patterns in either overall beetle activity or in the number of species recorded over the 17-year period, but there was a clear change from assemblages dominated by smaller species with higher dispersal capability to ones with larger beetles with less dispersal potential and a preference for less disturbed agroecosystems. The influence of surrounding crops on ground beetle activity in non-crop habitat has implications for ecosystem service provision by ground beetles as pest predators. These results are contrary to conventional assumptions and interpretations, which suggest activity of pest predators in crops is influenced primarily by adjacent non-crop habitat. The long-term nature of the assessment was important in elucidation of patterns and trends, and indicated that policies such as agri-environment schemes should take cropping patterns into account when promoting management options that are intended to enhance natural pest control.

  6. Crop cover the principal influence on non-crop ground beetle (Coleoptera, Carabidae) activity and assemblages at the farm scale in a long-term assessment.

    PubMed

    Eyre, M D; Sanderson, R A; McMillan, S D; Critchley, C N R

    2016-04-01

    Ground beetle data were generated using pitfall traps in the 17-year period from 1993 to 2009 and used to investigate the effects of changes in surrounding crop cover on beetle activity and assemblages, together with the effects of weather variability. Beetles were recorded from non-crop field margins (overgrown hedges). Crop cover changes explained far more variation in the beetle assemblages recorded than did temperature and rainfall variation. A reduction in management intensity and disturbance in the crops surrounding the traps, especially the introduction and development of willow coppice, was concomitant with changes in individual species activity and assemblage composition of beetles trapped in non-crop habitat. There were no consistent patterns in either overall beetle activity or in the number of species recorded over the 17-year period, but there was a clear change from assemblages dominated by smaller species with higher dispersal capability to ones with larger beetles with less dispersal potential and a preference for less disturbed agroecosystems. The influence of surrounding crops on ground beetle activity in non-crop habitat has implications for ecosystem service provision by ground beetles as pest predators. These results are contrary to conventional assumptions and interpretations, which suggest activity of pest predators in crops is influenced primarily by adjacent non-crop habitat. The long-term nature of the assessment was important in elucidation of patterns and trends, and indicated that policies such as agri-environment schemes should take cropping patterns into account when promoting management options that are intended to enhance natural pest control. PMID:26786247

  7. The Influence of Organized Physical Activity (including Gymnastics) on Young Adult Skeletal Traits: Is Maturity Phase Important?

    PubMed Central

    Bernardoni, Brittney; Scerpella, Tamara A.; Rosenbaum, Paula F.; Kanaley, Jill A.; Raab, Lindsay N.; Li, Quefeng; Wang, Sijian; Dowthwaite, Jodi N.

    2015-01-01

    We prospectively evaluated adolescent organized physical activity (PA) as a factor in adult female bone traits. Annual DXA scans accompanied semi-annual records of anthropometry, maturity and PA for 42 participants in this preliminary analysis (criteria: appropriately timed DXA scans at ~1 year pre-menarche [predictor] and ~5 years post-menarche [dependent variable]). Regression analysis evaluated total adolescent inter-scan PA and PA over 3 maturity sub-phases as predictors of young adult bone outcomes: 1) bone mineral content (BMC), geometry and strength indices at non-dominant distal radius and femoral neck; 2) sub-head BMC; 3) lumbar spine BMC. Analyses accounted for baseline gynecological age (years pre- or post-menarche), baseline bone status, adult body size and inter-scan body size change. Gymnastics training was evaluated as a potentially independent predictor, but did not improve models for any outcomes (p<0.07). Pre-menarcheal bone traits were strong predictors of most adult outcomes (semi-partial r2 = 0.21-0.59, p≤0.001). Adult 1/3 radius and sub-head BMC were predicted by both total PA and PA 1-3 years post-menarche (p<0.03). PA 3-5 years post-menarche predicted femoral narrow neck width, endosteal diameter and buckling ratio (p<0.05). Thus, participation in organized physical activity programs throughout middle and high school may reduce lifetime fracture risk in females. PMID:25386845

  8. The Influence of Organized Physical Activity (Including Gymnastics) on Young Adult Skeletal Traits: Is Maturity Phase Important?

    PubMed

    Bernardoni, Brittney; Scerpella, Tamara A; Rosenbaum, Paula F; Kanaley, Jill A; Raab, Lindsay N; Li, Quefeng; Wang, Sijian; Dowthwaite, Jodi N

    2015-05-01

    We prospectively evaluated adolescent organized physical activity (PA) as a factor in adult female bone traits. Annual DXA scans accompanied semiannual records of anthropometry, maturity, and PA for 42 participants in this preliminary analysis (criteria: appropriately timed DXA scans at ~1 year premenarche [predictor] and ~5 years postmenarche [dependent variable]). Regression analysis evaluated total adolescent interscan PA and PA over 3 maturity subphases as predictors of young adult bone outcomes: 1) bone mineral content (BMC), geometry, and strength indices at nondominant distal radius and femoral neck; 2) subhead BMC; 3) lumbar spine BMC. Analyses accounted for baseline gynecological age (years pre- or postmenarche), baseline bone status, adult body size and interscan body size change. Gymnastics training was evaluated as a potentially independent predictor, but did not improve models for any outcomes (p > .07). Premenarcheal bone traits were strong predictors of most adult outcomes (semipartial r2 = .21-0.59, p ≤ .001). Adult 1/3 radius and subhead BMC were predicted by both total PA and PA 1-3 years postmenarche (p < .03). PA 3-5 years postmenarche predicted femoral narrow neck width, endosteal diameter, and buckling ratio (p < .05). Thus, participation in organized physical activity programs throughout middle and high school may reduce lifetime fracture risk in females. PMID:25386845

  9. Development of a pneumatically driven active cover lid for multi-well microplates for use in perfusion three-dimensional cell culture

    NASA Astrophysics Data System (ADS)

    Huang, Song-Bin; Chou, Dean; Chang, Yu-Han; Li, Ke-Cing; Chiu, Tzu-Keng; Ventikos, Yiannis; Wu, Min-Hsien

    2015-12-01

    Before microfluidic-based cell culture models can be practically utilized for bioassays, there is a need for a transitional cell culture technique that can improve conventional cell culture models. To address this, a hybrid cell culture system integrating an active cover lid and a multi-well microplate was proposed to achieve perfusion 3-D cell culture. In this system, a microfluidic-based pneumatically-driven liquid transport mechanism was integrated into the active cover lid to realize 6-unit culture medium perfusion. Experimental results revealed that the flow of culture medium could be pneumatically driven in a flow-rate uniform manner. We used the system to successfully perform a perfusion 3-D cell culture of mesenchymal stem cells (MSCs) for up to 16 days. Moreover, we investigated the effects of various cell culture models on the physiology of MSCs. The physiological nature of MSCs can vary with respect to the cell culture model used. Using the perfusion 3-D cell culture format might affect the proliferation and osteogenic differentiation of MSCs. Overall, we have developed a cell culture system that can achieve multi-well microplate-based perfusion 3-D cell culture in an efficient, cost-effective, and user-friendly manner. These features could facilitate the widespread application of perfusion cell culture models for cell-based assays.

  10. Development of a pneumatically driven active cover lid for multi-well microplates for use in perfusion three-dimensional cell culture

    PubMed Central

    Huang, Song-Bin; Chou, Dean; Chang, Yu-Han; Li, Ke-Cing; Chiu, Tzu-Keng; Ventikos, Yiannis; Wu, Min-Hsien

    2015-01-01

    Before microfluidic-based cell culture models can be practically utilized for bioassays, there is a need for a transitional cell culture technique that can improve conventional cell culture models. To address this, a hybrid cell culture system integrating an active cover lid and a multi-well microplate was proposed to achieve perfusion 3-D cell culture. In this system, a microfluidic-based pneumatically-driven liquid transport mechanism was integrated into the active cover lid to realize 6-unit culture medium perfusion. Experimental results revealed that the flow of culture medium could be pneumatically driven in a flow-rate uniform manner. We used the system to successfully perform a perfusion 3-D cell culture of mesenchymal stem cells (MSCs) for up to 16 days. Moreover, we investigated the effects of various cell culture models on the physiology of MSCs. The physiological nature of MSCs can vary with respect to the cell culture model used. Using the perfusion 3-D cell culture format might affect the proliferation and osteogenic differentiation of MSCs. Overall, we have developed a cell culture system that can achieve multi-well microplate-based perfusion 3-D cell culture in an efficient, cost-effective, and user-friendly manner. These features could facilitate the widespread application of perfusion cell culture models for cell-based assays. PMID:26669749

  11. Development of a pneumatically driven active cover lid for multi-well microplates for use in perfusion three-dimensional cell culture.

    PubMed

    Huang, Song-Bin; Chou, Dean; Chang, Yu-Han; Li, Ke-Cing; Chiu, Tzu-Keng; Ventikos, Yiannis; Wu, Min-Hsien

    2015-01-01

    Before microfluidic-based cell culture models can be practically utilized for bioassays, there is a need for a transitional cell culture technique that can improve conventional cell culture models. To address this, a hybrid cell culture system integrating an active cover lid and a multi-well microplate was proposed to achieve perfusion 3-D cell culture. In this system, a microfluidic-based pneumatically-driven liquid transport mechanism was integrated into the active cover lid to realize 6-unit culture medium perfusion. Experimental results revealed that the flow of culture medium could be pneumatically driven in a flow-rate uniform manner. We used the system to successfully perform a perfusion 3-D cell culture of mesenchymal stem cells (MSCs) for up to 16 days. Moreover, we investigated the effects of various cell culture models on the physiology of MSCs. The physiological nature of MSCs can vary with respect to the cell culture model used. Using the perfusion 3-D cell culture format might affect the proliferation and osteogenic differentiation of MSCs. Overall, we have developed a cell culture system that can achieve multi-well microplate-based perfusion 3-D cell culture in an efficient, cost-effective, and user-friendly manner. These features could facilitate the widespread application of perfusion cell culture models for cell-based assays. PMID:26669749

  12. Effects of winter cover crops residue returning on soil enzyme activities and soil microbial community in double-cropping rice fields.

    PubMed

    Hai-Ming, Tang; Xiao-Ping, Xiao; Wen-Guang, Tang; Ye-Chun, Lin; Ke, Wang; Guang-Li, Yang

    2014-01-01

    Residue management in cropping systems is useful to improve soil quality. However, the studies on the effects of residue management on the enzyme activities and microbial community of soils in South China are few. Therefore, the effects of incorporating winter cover crop residue with a double-cropping rice (Oryza sativa L.) system on soil enzyme activities and microbial community in Southern China fields were studied. The experiment has conducted at the experimental station of the Institute of Soil and Fertilizer Research, Hunan Academy of Agricultural Science, China since winter 2004. Four winter cropping systems were used: rice-rice-ryegrass (Lolium multiflorum L.) (R-R-Ry), rice-rice-Chinese milk vetch (Astragalus sinicus L.) (R-R-Mv), rice-rice-rape (Brassica napus L.) (R-R-Ra) and rice-rice with winter fallow (R-R-Fa). The result indicated that the enzyme activities in the R-R-Ry, R-R-Mv and R-R-Ra systems were significantly higher (P<0.05) than in the R-R-Fa system during the early and late rice season. The β-glucosidase activities reached peak values at the tillering stage after residue application, and alkaline phosphatase activities reached peak values at the booting stage after residue application, respectively, the activities of β-glucosidase and alkaline phosphatase gradually decreased after this. Arylsulfatase activities reached peak values at the maturity stage. Arylamidase activities reached peak values at the maturity stage. The numbers of aerobic bacteria, actinomycete and fungus of residue treatments were significantly higher (P<0.05) than that the R-R-Ra system. However, the number of anaerobic bacteria under the R-R-Ry and R-R-Mv systems was significantly lower (P<0.05) than that under the R-R-Fa system during early rice and late rice growth stage. Thus, incorporation of winter cover crops into rotations may increase enzyme activities and microbial community in soil and therefore improve soil quality.

  13. Effects of Winter Cover Crops Residue Returning on Soil Enzyme Activities and Soil Microbial Community in Double-Cropping Rice Fields

    PubMed Central

    Hai-Ming, Tang; Xiao-Ping, Xiao; Wen-Guang, Tang; Ye-Chun, Lin; Ke, Wang; Guang-Li, Yang

    2014-01-01

    Residue management in cropping systems is useful to improve soil quality. However, the studies on the effects of residue management on the enzyme activities and microbial community of soils in South China are few. Therefore, the effects of incorporating winter cover crop residue with a double-cropping rice (Oryza sativa L.) system on soil enzyme activities and microbial community in Southern China fields were studied. The experiment has conducted at the experimental station of the Institute of Soil and Fertilizer Research, Hunan Academy of Agricultural Science, China since winter 2004. Four winter cropping systems were used: rice–rice–ryegrass (Lolium multiflorum L.) (R-R-Ry), rice–rice–Chinese milk vetch (Astragalus sinicus L.) (R-R-Mv), rice–rice–rape (Brassica napus L.) (R-R-Ra) and rice–rice with winter fallow (R-R-Fa). The result indicated that the enzyme activities in the R-R-Ry, R-R-Mv and R-R-Ra systems were significantly higher (P<0.05) than in the R-R-Fa system during the early and late rice season. The β-glucosidase activities reached peak values at the tillering stage after residue application, and alkaline phosphatase activities reached peak values at the booting stage after residue application, respectively, the activities of β-glucosidase and alkaline phosphatase gradually decreased after this. Arylsulfatase activities reached peak values at the maturity stage. Arylamidase activities reached peak values at the maturity stage. The numbers of aerobic bacteria, actinomycete and fungus of residue treatments were significantly higher (P<0.05) than that the R-R-Ra system. However, the number of anaerobic bacteria under the R-R-Ry and R-R-Mv systems was significantly lower (P<0.05) than that under the R-R-Fa system during early rice and late rice growth stage. Thus, incorporation of winter cover crops into rotations may increase enzyme activities and microbial community in soil and therefore improve soil quality. PMID:24956152

  14. Effects of winter cover crops residue returning on soil enzyme activities and soil microbial community in double-cropping rice fields.

    PubMed

    Hai-Ming, Tang; Xiao-Ping, Xiao; Wen-Guang, Tang; Ye-Chun, Lin; Ke, Wang; Guang-Li, Yang

    2014-01-01

    Residue management in cropping systems is useful to improve soil quality. However, the studies on the effects of residue management on the enzyme activities and microbial community of soils in South China are few. Therefore, the effects of incorporating winter cover crop residue with a double-cropping rice (Oryza sativa L.) system on soil enzyme activities and microbial community in Southern China fields were studied. The experiment has conducted at the experimental station of the Institute of Soil and Fertilizer Research, Hunan Academy of Agricultural Science, China since winter 2004. Four winter cropping systems were used: rice-rice-ryegrass (Lolium multiflorum L.) (R-R-Ry), rice-rice-Chinese milk vetch (Astragalus sinicus L.) (R-R-Mv), rice-rice-rape (Brassica napus L.) (R-R-Ra) and rice-rice with winter fallow (R-R-Fa). The result indicated that the enzyme activities in the R-R-Ry, R-R-Mv and R-R-Ra systems were significantly higher (P<0.05) than in the R-R-Fa system during the early and late rice season. The β-glucosidase activities reached peak values at the tillering stage after residue application, and alkaline phosphatase activities reached peak values at the booting stage after residue application, respectively, the activities of β-glucosidase and alkaline phosphatase gradually decreased after this. Arylsulfatase activities reached peak values at the maturity stage. Arylamidase activities reached peak values at the maturity stage. The numbers of aerobic bacteria, actinomycete and fungus of residue treatments were significantly higher (P<0.05) than that the R-R-Ra system. However, the number of anaerobic bacteria under the R-R-Ry and R-R-Mv systems was significantly lower (P<0.05) than that under the R-R-Fa system during early rice and late rice growth stage. Thus, incorporation of winter cover crops into rotations may increase enzyme activities and microbial community in soil and therefore improve soil quality. PMID:24956152

  15. New Pyrrole Derivatives with Potent Tubulin Polymerization Inhibiting Activity As Anticancer Agents Including Hedgehog-Dependent Cancer

    PubMed Central

    La Regina, Giuseppe; Bai, Ruoli; Coluccia, Antonio; Famiglini, Valeria; Pelliccia, Sveva; Passacantilli, Sara; Mazzoccoli, Carmela; Ruggieri, Vitalba; Sisinni, Lorenza; Bolognesi, Alessio; Rensen, Whilelmina Maria; Miele, Andrea; Nalli, Marianna; Alfonsi, Romina; Di Marcotullio, Lucia; Gulino, Alberto; Brancale, Andrea; Novellino, Ettore; Dondio, Giulio; Vultaggio, Stefania; Varasi, Mario; Mercurio, Ciro; Hamel, Ernest; Lavia, Patrizia; Silvestri, Romano

    2014-01-01

    We synthesized 3-aroyl-1-arylpyrrole (ARAP) derivatives as potential anticancer agents having different substituents at the pendant 1-phenyl ring. Both the 1-phenyl ring and 3-(3,4,5-trimethoxyphenyl)carbonyl moieties were mandatory to achieve potent inhibition of tubulin polymerization, binding of colchicine to tubulin, and cancer cell growth. ARAP 22 showed strong inhibition of the P-glycoprotein-overexpressing NCI-ADR-RES and Messa/Dx5MDR cell lines. Compounds 22 and 27 suppressed in vitro the Hedgehog signaling pathway, strongly reducing luciferase activity in SAG treated NIH3T3 Shh-Light II cells, and inhibited the growth of medulloblastoma D283 cells at nanomolar concentrations. ARAPs 22 and 27 represent a new potent class of tubulin polymerization and cancer cell growth inhibitors with the potential to inhibit the Hedgehog signaling pathway. PMID:25025991

  16. Short-Range Temporal Interactions in Sleep; Hippocampal Spike Avalanches Support a Large Milieu of Sequential Activity Including Replay.

    PubMed

    Mahoney, J Matthew; Titiz, Ali S; Hernan, Amanda E; Scott, Rod C

    2016-01-01

    Hippocampal neural systems consolidate multiple complex behaviors into memory. However, the temporal structure of neural firing supporting complex memory consolidation is unknown. Replay of hippocampal place cells during sleep supports the view that a simple repetitive behavior modifies sleep firing dynamics, but does not explain how multiple episodes could be integrated into associative networks for recollection during future cognition. Here we decode sequential firing structure within spike avalanches of all pyramidal cells recorded in sleeping rats after running in a circular track. We find that short sequences that combine into multiple long sequences capture the majority of the sequential structure during sleep, including replay of hippocampal place cells. The ensemble, however, is not optimized for maximally producing the behavior-enriched episode. Thus behavioral programming of sequential correlations occurs at the level of short-range interactions, not whole behavioral sequences and these short sequences are assembled into a large and complex milieu that could support complex memory consolidation.

  17. Short-Range Temporal Interactions in Sleep; Hippocampal Spike Avalanches Support a Large Milieu of Sequential Activity Including Replay.

    PubMed

    Mahoney, J Matthew; Titiz, Ali S; Hernan, Amanda E; Scott, Rod C

    2016-01-01

    Hippocampal neural systems consolidate multiple complex behaviors into memory. However, the temporal structure of neural firing supporting complex memory consolidation is unknown. Replay of hippocampal place cells during sleep supports the view that a simple repetitive behavior modifies sleep firing dynamics, but does not explain how multiple episodes could be integrated into associative networks for recollection during future cognition. Here we decode sequential firing structure within spike avalanches of all pyramidal cells recorded in sleeping rats after running in a circular track. We find that short sequences that combine into multiple long sequences capture the majority of the sequential structure during sleep, including replay of hippocampal place cells. The ensemble, however, is not optimized for maximally producing the behavior-enriched episode. Thus behavioral programming of sequential correlations occurs at the level of short-range interactions, not whole behavioral sequences and these short sequences are assembled into a large and complex milieu that could support complex memory consolidation. PMID:26866597

  18. Short-Range Temporal Interactions in Sleep; Hippocampal Spike Avalanches Support a Large Milieu of Sequential Activity Including Replay

    PubMed Central

    Mahoney, J. Matthew; Titiz, Ali S.; Hernan, Amanda E.; Scott, Rod C.

    2016-01-01

    Hippocampal neural systems consolidate multiple complex behaviors into memory. However, the temporal structure of neural firing supporting complex memory consolidation is unknown. Replay of hippocampal place cells during sleep supports the view that a simple repetitive behavior modifies sleep firing dynamics, but does not explain how multiple episodes could be integrated into associative networks for recollection during future cognition. Here we decode sequential firing structure within spike avalanches of all pyramidal cells recorded in sleeping rats after running in a circular track. We find that short sequences that combine into multiple long sequences capture the majority of the sequential structure during sleep, including replay of hippocampal place cells. The ensemble, however, is not optimized for maximally producing the behavior-enriched episode. Thus behavioral programming of sequential correlations occurs at the level of short-range interactions, not whole behavioral sequences and these short sequences are assembled into a large and complex milieu that could support complex memory consolidation. PMID:26866597

  19. Product and rate determinations with chemically activated nucleotides in the presence of various prebiotic materials, including other mono- and polynucleotides

    NASA Technical Reports Server (NTRS)

    Kanavarioti, A.; Alberas, D. J.; Rosenbach, M. T.; Bernasconi, C. F.; Chang, S.

    1991-01-01

    We are investigating the reactions of ImpN's in the presence of a number of prebiotically plausible materials, such as metal ions, phosphate, amines and other nucleotides and hope to learn more about the stability/reactivity of ImpN's in a prebiotic aqueous environment. We find that, in the presence of phosphate, ImpN's form substantial amounts of diphosphate nucleotides. These diphosphate nucleotides are not very good substrates for template directed reactions, but are chemically activated and are known to revert to the phosphoimidazolides in the presence of imidazole under solid state conditions. With respect to our studies of the oligomerization reaction, the determination of the dimerization rate constant of a specific ImpN (guanosine 5'-phospho 2 methylimidazolide) both in the absence and the presence of the template leads to the conclusion that at 37 C the dimerization is not template directed, although the subsequent polymerization steps are. In other words, this specific polynucleotide synthesizing system favors the elongation of oligonucleotides as compared with the formation of dimers and trimers. This favoring of the synthesis of long as opposed to short oligonucleotides may be regarded as a rudimentary example of natural selection at the molecular level.

  20. A comparison of methods of assessment of body composition including neutron activation analysis of total body nitrogen.

    PubMed

    Lukaski, H C; Mendez, J; Buskirk, E R; Cohn, S H

    1981-08-01

    Fourteen healthy men underwent determinations of total body nitrogen (TBN) by prompt gamma neutron activation analysis and total body potassium (TBK) by whole body counting to estimate the muscle and nonmuscle components of the fat-free body mass (FFBM) and their protein contents. Comparison of FFBM estimated from TBN and TBK (60.6 +/- 6.9 kg, mean +/- SD), densitometry (62.3 +/- 7.1 kg), TBK alone (62.2 +/- 8.0 kg) and TBW (63.9 +/- 7.8 kg) showed no differences among the techniques. Similarly, there were neither differences in fat mass nor percent body fat among the methods. Analysis of the chemical composition of FFBM of this group showed TBK/FFBM = 62.6 +/- 2.3 mEq/kg, TBW/FFBM = 74.6 +/- 0.2%, TBN/FFBM = 32.74 +/- 1.09 g/kg, protein/FFBM = 20.5+/- 0.7%. The calculated mineral content of the FFBM was 6.4%. These values are strikingly similar to the values calculated by direct chemical analysis. It was concluded that the combined TBN-TBK method is a valid technique for estimating body composition in man.

  1. Selective Non-nucleoside Inhibitors of Human DNA Methyltransferases Active in Cancer Including in Cancer Stem Cells

    PubMed Central

    2015-01-01

    DNA methyltransferases (DNMTs) are important enzymes involved in epigenetic control of gene expression and represent valuable targets in cancer chemotherapy. A number of nucleoside DNMT inhibitors (DNMTi) have been studied in cancer, including in cancer stem cells, and two of them (azacytidine and decitabine) have been approved for treatment of myelodysplastic syndromes. However, only a few non-nucleoside DNMTi have been identified so far, and even fewer have been validated in cancer. Through a process of hit-to-lead optimization, we report here the discovery of compound 5 as a potent non-nucleoside DNMTi that is also selective toward other AdoMet-dependent protein methyltransferases. Compound 5 was potent at single-digit micromolar concentrations against a panel of cancer cells and was less toxic in peripheral blood mononuclear cells than two other compounds tested. In mouse medulloblastoma stem cells, 5 inhibited cell growth, whereas related compound 2 showed high cell differentiation. To the best of our knowledge, 2 and 5 are the first non-nucleoside DNMTi tested in a cancer stem cell line. PMID:24387159

  2. Novel stilbenoids, including cannabispiradienone glycosides, from Tragopogon tommasinii (Asteraceae, Cichorieae) and their potential anti-inflammatory activity.

    PubMed

    Granica, Sebastian; Piwowarski, Jakub P; Randazzo, Antonio; Schneider, Peter; Żyżyńska-Granica, Barbara; Zidorn, Christian

    2015-09-01

    A phytochemical investigation of Tragopogon tommasinii Sch.Bip. (Asteraceae, Cichorieae) yielded a total of 21 natural products, two simple phenolic acids (4-hydroxybenzoic acid and p-coumaric acid), four caffeic acid derivatives (chlorogenic acid, 3-O-caffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid), six flavonoids (luteolin, luteolin 7-O-glucoside, vitexin, orientin, quercetin 3-O-glucoside, and isorhamnetin 3-O-glucoside), three simple bibenzyls [2-carboxyl-5-hydroxy-3-methoxy-4'-β-glucopyranosyl-oxybibenzyl, 3-caffeoyl-(9→5)-β-apiosyl-(1→6)-β-glucopyranosyloxy-5,4'-dihydroxy-3'-methoxybibenzyl, 3-caffeoyl-(9→5)-β-apiosyl-(1→6)-β-glucopyranosyloxy-4'-dihydroxy-5,3'-dimethoxybibenzyl], three phtalides [3-(4-β-glucopyranosyloxybenzyl)-7-hydroxy-5-methoxyphtalide, 7-β-glucopyranosyloxy-(S)-3-(4-hydroxybenzyl)-5-methoxyphtalide, and 7-(1→6)-α-rhamnosyl-β-glucopyranosyloxy-(S)-3-(4-hydroxybenzyl)-5-methoxyphtalide], two cannabispiradienone derivatives [3-O-β-glucopyranosyldemethoxycannabispiradienone and 3-caffeoyl-(9→5)-β-apiosyl-(1→6)-β-glucopyranosyloxydemethoxycannabispiradienone], and tetra-N-coumaroyl spermine. The three bibenzyls, the latter two benzylphthalides, and both cannabispiradienone derivatives represent new natural compounds and all compounds, except the caffeic acid derivatives and the flavonoids were new for T. tommasinii. The structures were established by HR mass spectrometry, extensive 1D and 2D NMR spectroscopy, and CD spectroscopy. Moreover, the potential anti-inflammatory activities of the new compounds were assayed using human neutrophils and their production of IL-1b, IL-8, TNF-α and MMP-9 as well as the expression of TLR-4, respectively.

  3. Snow-cover dynamics monitored by automatic digital photography at the rooting zone of an active rock glacier in the Hinteres Lantal Cirque, Austria

    NASA Astrophysics Data System (ADS)

    Kellerer-Pirklbauer, Andreas; Rieckh, Matthias; Avian, Michael

    2010-05-01

    Knowledge regarding snow-cover dynamics and climatic conditions in the rooting zone of active rock glaciers is still limited. The number of meteorological stations on the surface of or close to active rock glaciers is increasing. However, areal information on snow-cover distribution and its spatial dynamics caused by different processes on rock glaciers surfaces with a high temporal resolution from such remote alpine areas are mostly difficult to obtain. To face this problem an automatic remote digital camera (RDC) system was proprietary developed. The core parts of the RDC system are a standard hand-held digital camera, a remote control, a water proof casing with a transparent opening, a 12V/25Ah battery and solar panels with a charge controller. Three such devices were constructed and installed at different sites in the Central Alps of Austria. One RDC system is used to monitor the rooting zone of the highly active rock glacier in the Hinteres Langtal Cirque (46°59'N, 12°47'E), Central Schober Mountains, Austria. The 0.15 km² large NW-facing rock glaciers is tongue-shaped with a fast moving lower part (>1m/a) and a substantially slower upper part, ranging in elevation between 2455-2700 m a.s.l. The RDC system was set up in September 2006 and is located since than at 2770 m a.s.l. on a pronounced ridge crest that confines the Hinteres Langtal Cirque to the SW. The water proof casing was attached to a 1.5 m high metal pole which itself was fixed to the bedrock by screws and concrete glue. The viewing direction of the camera is NE. Hence, the image section of the RDC focuses on the rooting zone of the rock glacier and its headwalls up to c. 3000 m a.s.l. Photographs were taken daily at 3 pm providing the optimal lighting conditions in the relevant part of the cirque. 720 photographs were taken continuously in the period 12.09.2006 to 31.08.2008. These optical data were analysed by applying GIS and remote sensing techniques regarding snow-cover distribution

  4. High water-stressed population estimated by world water resources assessment including human activities under SRES scenarios

    NASA Astrophysics Data System (ADS)

    Kiguchi, M.; Shen, Y.; Kanae, S.; Oki, T.

    2009-04-01

    In an argument of the reduction and the adaptation for the climate change, the evaluation of the influence by the climate change is important. When we argue in adaptation plan from a damage scale and balance with the cost, it is particularly important. Parry et al (2001) evaluated the risks in shortage of water, malaria, food, the risk of the coast flood by temperature function and clarified the level of critical climate change. According to their evaluation, the population to be affected by the shortage of water suddenly increases in the range where temperature increases from 1.5 to 2.0 degree in 2080s. They showed how much we need to reduce emissions in order to draw-down significantly the number at risk. This evaluation of critical climate change threats and targets of water shortage did not include the water withdrawal divided by water availability. Shen et al (2008a) estimated the water withdrawal of projection of future world water resources according to socio-economic driving factors predicted for scenarios A1b, A2, B1, and B2 of the Special Report on Emission Scenarios (SRES). However, these results were in function of not temperature but time. The assessment of the highly water-stressed population considered the socioeconomic development is necessary for a function of the temperature. Because of it is easy to understand to need to reduce emission. We present a multi-GCM analysis of the global and regional populations lived in highly water-stressed basin for a function of the temperature using the socioeconomic data and the outputs of GCMs. In scenario A2, the population increases gradually with warming. On the other hand, the future projection population in scenario A1b and B1 increase gradually until the temperature anomaly exceeds around from +1 to +1.5 degree. After that the population is almost constant. From Shen et al (2008b), we evaluated the HWSP and its ratio in the world with temperature function for scenarios A1B, A2, and B1 by the index of W

  5. ISEE 3 observations during the CDAW 8 intervals - Case studies of the distant geomagnetic tail covering a wide range of geomagnetic activity

    NASA Technical Reports Server (NTRS)

    Richardson, I. G.; Slavin, J. A.; Owen, C. J.; Cowley, S. W. H.; Galvin, A. B.; Sanderson, T. R.; Scholer, M.

    1989-01-01

    Observations made by the ISEE 3 spacecraft in the distant geomagnetic tail during the eight CDAW 8 intervals are discussed, along with their relation to concurrent geomagnetic activity. This extensive multiinstrument case study of distant tail data covers a wide range of geomagnetic conditions from extended intervals of magnetic quiet with isolated substorms to prolonged periods of intense disturbance. Plasmoids are observed in the distant tail following disturbance enhancements, the time of their appearance being generally consistent with disconnection from the near-earth region at the time of the enhancement. Their structure is entirely consistent with the neutral line model. However, not all enhancements in geomagnetic activity result in the observation of plasmoids. In particular, the CDAW 8 data suggest that, during extended intervals of strong activity, a continuous neutral line may reside in the near-earth tail and some disturbance enhancements may then relate to an increase in the reconnection rate at a preexisting neutral line, rather than to new neutral line and plasmoid formation.

  6. Land Cover Indicators for U.S. National Climate Assessments

    NASA Astrophysics Data System (ADS)

    Channan, S.; Thomson, A. M.; Collins, K. M.; Sexton, J. O.; Torrens, P.; Emanuel, W. R.

    2014-12-01

    Land is a critical resource for human habitat and for the vast majority of human activities. Many natural resources are derived from terrestrial ecosystems or otherwise extracted from the landscape. Terrestrial biodiversity depends on land attributes as do people's perceptions of the value of land, including its value for recreation or tourism. Furthermore, land surface properties and processes affect weather and climate, and land cover change and land management affect emissions of greenhouse gases. Thus, land cover with its close association with climate is so pervasive that a land cover indicator is of fundamental importance to U.S. national climate assessments and related research. Moderate resolution remote sensing products (MODIS) were used to provide systematic data on annual distributions of land cover over the period 2001-2012. Selected Landsat observations and data products further characterize land cover at higher resolution. Here we will present the prototype for a suite of land cover indicators including land cover maps as well as charts depicting attributes such as composition by land cover class, statistical indicators of landscape characteristics, and tabular data summaries indispensable for communicating the status and trends of U.S. land cover at national, regional and state levels.

  7. A3 domain region 1803-1818 contributes to the stability of activated factor VIII and includes a binding site for activated factor IX.

    PubMed

    Bloem, Esther; Meems, Henriet; van den Biggelaar, Maartje; Mertens, Koen; Meijer, Alexander B

    2013-09-01

    A recent chemical footprinting study in our laboratory suggested that region 1803-1818 might contribute to A2 domain retention in activated factor VIII (FVIIIa). This site has also been implicated to interact with activated factor IX (FIXa). Asn-1810 further comprises an N-linked glycan, which seems incompatible with a role of the amino acids 1803-1818 for FIXa or A2 domain binding. In the present study, FVIIIa stability and FIXa binding were evaluated in a FVIII-N1810C variant, and two FVIII variants in which residues 1803-1810 and 1811-1818 are replaced by the corresponding residues of factor V (FV). Enzyme kinetic studies showed that only FVIII/FV 1811-1818 has a decreased apparent binding affinity for FIXa. Flow cytometry analysis indicated that fluorescent FIXa exhibits impaired complex formation with only FVIII/FV 1811-1818 on lipospheres. Site-directed mutagenesis revealed that Phe-1816 contributes to the interaction with FIXa. To evaluate FVIIIa stability, the FVIII/FV chimeras were activated by thrombin, and the decline in cofactor function was followed over time. FVIII/FV 1803-1810 and FVIII/FV 1811-1818 but not FVIII-N1810C showed a decreased FVIIIa half-life. However, when the FVIII variants were activated in presence of FIXa, only FVIII/FV 1811-1818 demonstrated an enhanced decline in cofactor function. Surface plasmon resonance analysis revealed that the FVIII variants K1813A/K1818A, E1811A, and F1816A exhibit enhanced dissociation after activation. The results together demonstrate that the glycan at 1810 is not involved in FVIII cofactor function, and that Phe-1816 of region 1811-1818 contributes to FIXa binding. Both regions 1803-1810 and 1811-1818 contribute to FVIIIa stability.

  8. A3 domain region 1803-1818 contributes to the stability of activated factor VIII and includes a binding site for activated factor IX.

    PubMed

    Bloem, Esther; Meems, Henriet; van den Biggelaar, Maartje; Mertens, Koen; Meijer, Alexander B

    2013-09-01

    A recent chemical footprinting study in our laboratory suggested that region 1803-1818 might contribute to A2 domain retention in activated factor VIII (FVIIIa). This site has also been implicated to interact with activated factor IX (FIXa). Asn-1810 further comprises an N-linked glycan, which seems incompatible with a role of the amino acids 1803-1818 for FIXa or A2 domain binding. In the present study, FVIIIa stability and FIXa binding were evaluated in a FVIII-N1810C variant, and two FVIII variants in which residues 1803-1810 and 1811-1818 are replaced by the corresponding residues of factor V (FV). Enzyme kinetic studies showed that only FVIII/FV 1811-1818 has a decreased apparent binding affinity for FIXa. Flow cytometry analysis indicated that fluorescent FIXa exhibits impaired complex formation with only FVIII/FV 1811-1818 on lipospheres. Site-directed mutagenesis revealed that Phe-1816 contributes to the interaction with FIXa. To evaluate FVIIIa stability, the FVIII/FV chimeras were activated by thrombin, and the decline in cofactor function was followed over time. FVIII/FV 1803-1810 and FVIII/FV 1811-1818 but not FVIII-N1810C showed a decreased FVIIIa half-life. However, when the FVIII variants were activated in presence of FIXa, only FVIII/FV 1811-1818 demonstrated an enhanced decline in cofactor function. Surface plasmon resonance analysis revealed that the FVIII variants K1813A/K1818A, E1811A, and F1816A exhibit enhanced dissociation after activation. The results together demonstrate that the glycan at 1810 is not involved in FVIII cofactor function, and that Phe-1816 of region 1811-1818 contributes to FIXa binding. Both regions 1803-1810 and 1811-1818 contribute to FVIIIa stability. PMID:23884417

  9. A3 Domain Region 1803–1818 Contributes to the Stability of Activated Factor VIII and Includes a Binding Site for Activated Factor IX

    PubMed Central

    Bloem, Esther; Meems, Henriet; van den Biggelaar, Maartje; Mertens, Koen; Meijer, Alexander B.

    2013-01-01

    A recent chemical footprinting study in our laboratory suggested that region 1803–1818 might contribute to A2 domain retention in activated factor VIII (FVIIIa). This site has also been implicated to interact with activated factor IX (FIXa). Asn-1810 further comprises an N-linked glycan, which seems incompatible with a role of the amino acids 1803–1818 for FIXa or A2 domain binding. In the present study, FVIIIa stability and FIXa binding were evaluated in a FVIII-N1810C variant, and two FVIII variants in which residues 1803–1810 and 1811–1818 are replaced by the corresponding residues of factor V (FV). Enzyme kinetic studies showed that only FVIII/FV 1811–1818 has a decreased apparent binding affinity for FIXa. Flow cytometry analysis indicated that fluorescent FIXa exhibits impaired complex formation with only FVIII/FV 1811–1818 on lipospheres. Site-directed mutagenesis revealed that Phe-1816 contributes to the interaction with FIXa. To evaluate FVIIIa stability, the FVIII/FV chimeras were activated by thrombin, and the decline in cofactor function was followed over time. FVIII/FV 1803–1810 and FVIII/FV 1811–1818 but not FVIII-N1810C showed a decreased FVIIIa half-life. However, when the FVIII variants were activated in presence of FIXa, only FVIII/FV 1811–1818 demonstrated an enhanced decline in cofactor function. Surface plasmon resonance analysis revealed that the FVIII variants K1813A/K1818A, E1811A, and F1816A exhibit enhanced dissociation after activation. The results together demonstrate that the glycan at 1810 is not involved in FVIII cofactor function, and that Phe-1816 of region 1811–1818 contributes to FIXa binding. Both regions 1803–1810 and 1811–1818 contribute to FVIIIa stability. PMID:23884417

  10. Preschool Curriculum: A Child Centered Curriculum of Concepts and Activities Including All of the CDA Functional Areas--Bi-lingual and Home Activities.

    ERIC Educational Resources Information Center

    Cunningham, Dorothy, Comp.

    This preschool resource curriculum, intended for use in a Head Start center, was written to correspond with a work plan and curriculum for children 3 to 5 years of age. The units included in the curriculum guide are aimed at integrating growth experiences with the learning of basic skills. Each of the 16 units contains material related to each of…

  11. Rapid binding of a cationic active site inhibitor to wild type and mutant mouse acetylcholinesterase: Brownian dynamics simulation including diffusion in the active site gorge.

    PubMed

    Tara, S; Elcock, A H; Kirchhoff, P D; Briggs, J M; Radic, Z; Taylor, P; McCammon, J A

    1998-12-01

    It is known that anionic surface residues play a role in the long-range electrostatic attraction between acetylcholinesterase and cationic ligands. In our current investigation, we show that anionic residues also play an important role in the behavior of the ligand within the active site gorge of acetylcholinesterase. Negatively charged residues near the gorge opening not only attract positively charged ligands from solution to the enzyme, but can also restrict the motion of the ligand once it is inside of the gorge. We use Brownian dynamics techniques to calculate the rate constant kon, for wild type and mutant acetylcholinesterase with a positively charged ligand. These calculations are performed by allowing the ligand to diffuse within the active site gorge. This is an extension of previously reported work in which a ligand was allowed to diffuse only to the enzyme surface. By setting the reaction criteria for the ligand closer to the active site, better agreement with experimental data is obtained. Although a number of residues influence the movement of the ligand within the gorge, Asp74 is shown to play a particularly important role in this function. Asp74 traps the ligand within the gorge, and in this way helps to ensure a reaction.

  12. A poultry-intestinal isolate of Campylobacter jejuni produces a bacteriocin (CUV-3) active against a range of Gram positive bacterial pathogens including Clostridium perfringens

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A newly isolated bacteriocin, CUV-3, produced by a poultry cecal isolate of Campylobacter jejuni strain CUV-3 had inhibitory activity against several Gram positive bacteria including Clostridium perfringens (38 strains), Staphylococcus aureus, Staph.epidermidis and Listeria monocytogenes. The pept...

  13. Including Youth with Intellectual Disabilities in Health Promotion Research: Development and Reliability of a Structured Interview to Assess the Correlates of Physical Activity among Youth

    ERIC Educational Resources Information Center

    Curtin, Carol; Bandini, Linda G.; Must, Aviva; Phillips, Sarah; Maslin, Melissa C. T.; Lo, Charmaine; Gleason, James M.; Fleming, Richard K.; Stanish, Heidi I.

    2016-01-01

    Background: The input of youth with intellectual disabilities in health promotion and health disparities research is essential for understanding their needs and preferences. Regular physical activity (PA) is vital for health and well-being, but levels are low in youth generally, including those with intellectual disabilities. Understanding the…

  14. Multiple layer insulation cover

    DOEpatents

    Farrell, James J.; Donohoe, Anthony J.

    1981-11-03

    A multiple layer insulation cover for preventing heat loss in, for example, a greenhouse, is disclosed. The cover is comprised of spaced layers of thin foil covered fabric separated from each other by air spaces. The spacing is accomplished by the inflation of spaced air bladders which are integrally formed in the cover and to which the layers of the cover are secured. The bladders are inflated after the cover has been deployed in its intended use to separate the layers of the foil material. The sizes of the material layers are selected to compensate for sagging across the width of the cover so that the desired spacing is uniformly maintained when the cover has been deployed. The bladders are deflated as the cover is stored thereby expediting the storage process and reducing the amount of storage space required.

  15. Measurement of factor VIII activity using one-stage clotting assay: a calibration curve has not to be systematically included in each run.

    PubMed

    Lattes, S; Appert-Flory, A; Fischer, F; Jambou, D; Toulon, P

    2011-01-01

    Coagulation factor VIII (FVIII) is usually evaluated using activated partial thromboplastin time-based one-stage clotting assays. Guidelines for clotting factor assays indicate that a calibration curve should be included each time the assay is performed. Therefore, FVIII measurement is expensive, reagent- and time-consuming. The aim of this study was to compare FVIII activities obtained using the same fully automated assay that was calibrated once (stored calibration curve) or each time the assay was performed. Unique lots of reagents were used throughout the study. We analysed 255 frozen plasma samples from patients who were prescribed FVIII measurement including treated and untreated haemophilia A patients. Twenty-six runs were performed on a 28-week period, each including four lyophilized control and at most 10 patient plasma samples. In control samples, FVIII activities were not significantly different when the assay was performed using the stored calibration curve or was daily calibrated. The same applied to FVIII activities in patient plasma samples that were not significantly different throughout the measuring range of activities [68.3% (<1-179) vs. 67.6% (<1-177), P=0.48] and no relevant bias could be demonstrated when data were compared according to Bland and Altman. These results suggest that in the studied technical conditions, performing the FVIII assay using a stored calibration curve is reliable, for at least 6 months. Therefore, as far as the same lots of reagents are used, it is not mandatory to include a calibration curve each time the FVIII assay was performed. However, this strategy has to be validated if the assay is performed in different technical conditions.

  16. The impact of human activities on land use and land cover changes and environmental processes in the Gorce Mountains (Western Polish Carpathians) in the past 50 years.

    PubMed

    Bucała, Anna

    2014-06-01

    The role of human impact on the natural environment was studied in the Jaszcze and Jamne catchments in the Gorce Mountains (Western Polish Carpathians). Analysis of land use and land cover changes using GIS techniques and cartographic materials between 1954 and 2004 indicates an increase in forest area by 11.5% and 18.5%, respectively, at the expense of arable land and grassland areas. Agricultural abandonment often occurred on steep slopes (above 10°) with skeletal (shallow) soils at higher elevations (above 800 m a.s.l.). In addition, the density of dirt roads decreased from 6.97 km/km(2) in 1981 to 4.3 km/km(2) in 2008. In former agricultural areas, long expanses of terraces have either disappeared or experienced shallow mass movements. The statistical reports and questionnaire survey indicate reduced income from farm activities in this region. As a result of LULC changes and stream transformation, the Jaszcze and Jamne stream channels were shortened, straightened, and narrowed, with tendency to incision estimated at 1 cm per year over the past 40 years. The changes observed in the environment under human impact, accelerated following 1989, are representative of the Western Polish Carpathians. PMID:24565936

  17. Cover Your Cough

    MedlinePlus

    ... What's this? Submit Button Past Newsletters Cover Your Cough Language: English Español Recommend on Facebook Tweet ... Posters only available as PDF files. Cover Your Cough, Flyer for Health Care Settings English [324 KB] ...

  18. Cover crops for Alabama

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cover crops are grown to benefit the following crop as well as to improve the soil, but they are normally not intended for harvest. Selecting the right cover crops for farming operations can improve yields, soil and water conservation and quality, and economic productivity. Properly managed cover ...

  19. Comparison of a coupled snow thermodynamic and radiative transfer model with in situ active microwave signatures of snow-covered smooth first-year sea ice

    NASA Astrophysics Data System (ADS)

    Fuller, M. C.; Geldsetzer, T.; Yackel, J.; Gill, J. P. S.

    2015-11-01

    Within the context of developing data inversion and assimilation techniques for C-band backscatter over sea ice, snow physical models may be used to drive backscatter models for comparison and optimization with satellite observations. Such modeling has the potential to enhance understanding of snow on sea-ice properties required for unambiguous interpretation of active microwave imagery. An end-to-end modeling suite is introduced, incorporating regional reanalysis data (NARR), a snow model (SNTHERM89.rev4), and a multilayer snow and ice active microwave backscatter model (MSIB). This modeling suite is assessed against measured snow on sea-ice geophysical properties and against measured active microwave backscatter. NARR data were input to the SNTHERM snow thermodynamic model in order to drive the MSIB model for comparison to detailed geophysical measurements and surface-based observations of C-band backscatter of snow on first-year sea ice. The NARR variables were correlated to available in situ measurements with the exception of long-wave incoming radiation and relative humidity, which impacted SNTHERM simulations of snow temperature. SNTHERM snow grain size and density were comparable to observations. The first assessment of the forward assimilation technique developed in this work required the application of in situ salinity profiles to one SNTHERM snow profile, which resulted in simulated backscatter close to that driven by in situ snow properties. In other test cases, the simulated backscatter remained 4-6 dB below observed for higher incidence angles and when compared to an average simulated backscatter of in situ end-member snow covers. Development of C-band inversion and assimilation schemes employing SNTHERM89.rev4 should consider sensitivity of the model to bias in incoming long-wave radiation, the effects of brine, and the inability of SNTHERM89.Rev4 to simulate water accumulation and refreezing at the bottom and mid-layers of the snowpack. These impact

  20. 43 CFR 5.1 - What does this subpart cover?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... PROJECTS AND STILL PHOTOGRAPHY ON CERTAIN AREAS UNDER DEPARTMENT JURISDICTION Areas Administered by the... does this subpart cover? This subpart covers commercial filming and still photography activities...

  1. 43 CFR 5.1 - What does this subpart cover?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... PROJECTS AND STILL PHOTOGRAPHY ON CERTAIN AREAS UNDER DEPARTMENT JURISDICTION Areas Administered by the... does this subpart cover? This subpart covers commercial filming and still photography activities...

  2. Applying the Model of Goal-Directed Behavior, Including Descriptive Norms, to Physical Activity Intentions: A Contribution to Improving the Theory of Planned Behavior.

    PubMed

    Esposito, Gabriele; van Bavel, René; Baranowski, Tom; Duch-Brown, Néstor

    2016-08-01

    The theory of planned behavior (TPB) has received its fair share of criticism lately, including calls for it to retire. We contribute to improving the theory by testing extensions such as the model of goal-directed behavior (MGDB, which adds desire and anticipated positive and negative emotions) applied to physical activity (PA) intention. We also test the inclusion of a descriptive norms construct as an addition to the subjective norms construct, also applied to PA, resulting in two additional models: TPB including descriptive norms (TPB + DN) and MGDB including descriptive norms (MGDB + DN). The study is based on an online survey of 400 young adult Internet users, previously enrolled in a subject pool. Confirmatory factor analysis (CFA) showed that TPB and TPB + DN were not fit for purpose, while MGDB and MGDB + DN were. Structural equation modelling (SEM) conducted on MGDB and MGDB + DN showed that the inclusion of descriptive norms took over the significance of injunctive norms, and increased the model's account of total variance in intention to be physically active. PMID:27229344

  3. Next generation of global land cover characterization, mapping, and monitoring

    NASA Astrophysics Data System (ADS)

    Giri, C.; Pengra, B.; Long, J.; Loveland, T. R.

    2013-12-01

    Land cover change is increasingly affecting the biophysics, biogeochemistry, and biogeography of the Earth's surface and the atmosphere, with far-reaching consequences to human well-being. However, our scientific understanding of the distribution and dynamics of land cover and land cover change (LCLCC) is limited. Previous global land cover assessments performed using coarse spatial resolution (300 m-1 km) satellite data did not provide enough thematic detail or change information for global change studies and for resource management. High resolution (˜30 m) land cover characterization and monitoring is needed that permits detection of land change at the scale of most human activity and offers the increased flexibility of environmental model parameterization needed for global change studies. However, there are a number of challenges to overcome before producing such data sets including unavailability of consistent global coverage of satellite data, sheer volume of data, unavailability of timely and accurate training and validation data, difficulties in preparing image mosaics, and high performance computing requirements. Integration of remote sensing and information technology is needed for process automation and high-performance computing needs. Recent developments in these areas have created an opportunity for operational high resolution land cover mapping, and monitoring of the world. Here, we report and discuss these advancements and opportunities in producing the next generations of global land cover characterization, mapping, and monitoring at 30-m spatial resolution primarily in the context of United States, Group on Earth Observations Global 30 m land cover initiative (UGLC).

  4. Wheelspace windage cover plate for turbine

    DOEpatents

    Lathrop, Norman Douglas

    2002-01-01

    Windage cover plates are secured between the wheels and spacer of a turbine rotor to prevent hot flow path gas ingestion into the wheelspace cavities. Each cover plate includes a linear, axially extending body curved circumferentially with a radially outwardly directed wall at one axial end. The wall defines a axially opening recess for receiving a dovetail lug. The cover plate includes an axially extending tongue received in a circumferential groove of the spacer. The cover plate is secured with the tongue in the groove and dovetail lug in the recess. Lap joints between circumferentially adjacent cover plates are provided.

  5. Verrucous carcinomas of the head and neck, including those with associated squamous cell carcinoma, lack transcriptionally active high-risk human papillomavirus.

    PubMed

    Patel, Kalyani R; Chernock, Rebecca D; Zhang, Tian R; Wang, Xiaowei; El-Mofty, Samir K; Lewis, James S

    2013-11-01

    Most oropharyngeal squamous cell carcinomas (SCC) and histologic variants harbor transcriptionally active human papillomavirus (HPV). While HPV DNA can be found in many non-oropharyngeal head and neck carcinomas, transcriptionally active HPV is rare. Verrucous carcinoma is a variant with bland cytology, warty appearance, locally destructive growth, and lack of metastasis when lacking a frankly invasive carcinoma component. Studies have shown variable rates of HPV DNA and p16 protein expression in such tumors but still have not clearly addressed if the virus has biological activity or clinical relevance in the positive cases. Department files were searched for verrucous neoplasms, including pure verrucous carcinoma, verrucous carcinoma with dysplasia or minimal invasion, and SCC arising in verrucous carcinoma (ie, having a major component of frankly invasive carcinoma). p16 immunohistochemistry, HPV DNA polymerase chain reaction (PCR) and E6/E7 mRNA reverse transcription PCR for high-risk HPV types were performed. Of the 49 cases, 6 (12.2%) showed strong (>50%) staining for p16. HPV DNA was detected in 7/49 (14.3%) cases, but only one case was positive for both p16, and HPV DNA. A total of 36 cases yielded sufficient RNA for RT-PCR (18 verrucous carcinomas, 13 atypical verrucous carcinomas, and 5 SCC arising in verrucous carcinoma). All 36 were negative, including the four p16-positive and three HPV DNA-positive tumors tested. Although a minority of verrucous carcinoma lesions are p16 and HPV DNA positive, transcriptionally active high-risk HPV is uniformly absent. These findings argue that verrucous carcinoma and its related squamous cell carcinomas are not HPV-driven tumors.

  6. Land-cover change detection

    USGS Publications Warehouse

    Chen, Xuexia; Giri, Chandra; Vogelmann, James

    2012-01-01

    Land cover is the biophysical material on the surface of the earth. Land-cover types include grass, shrubs, trees, barren, water, and man-made features. Land cover changes continuously.  The rate of change can be either dramatic and abrupt, such as the changes caused by logging, hurricanes and fire, or subtle and gradual, such as regeneration of forests and damage caused by insects (Verbesselt et al., 2001).  Previous studies have shown that land cover has changed dramatically during the past sevearal centuries and that these changes have severely affected our ecosystems (Foody, 2010; Lambin et al., 2001). Lambin and Strahlers (1994b) summarized five types of cause for land-cover changes: (1) long-term natural changes in climate conditions, (2) geomorphological and ecological processes, (3) human-induced alterations of vegetation cover and landscapes, (4) interannual climate variability, and (5) human-induced greenhouse effect.  Tools and techniques are needed to detect, describe, and predict these changes to facilitate sustainable management of natural resources.

  7. Optical modulator including grapene

    DOEpatents

    Liu, Ming; Yin, Xiaobo; Zhang, Xiang

    2016-06-07

    The present invention provides for a one or more layer graphene optical modulator. In a first exemplary embodiment the optical modulator includes an optical waveguide, a nanoscale oxide spacer adjacent to a working region of the waveguide, and a monolayer graphene sheet adjacent to the spacer. In a second exemplary embodiment, the optical modulator includes at least one pair of active media, where the pair includes an oxide spacer, a first monolayer graphene sheet adjacent to a first side of the spacer, and a second monolayer graphene sheet adjacent to a second side of the spacer, and at least one optical waveguide adjacent to the pair.

  8. Armored Geomembrane Cover Engineering

    PubMed Central

    Foye, Kevin

    2011-01-01

    Geomembranes are an important component of modern engineered barriers to prevent the infiltration of stormwater and runoff into contaminated soil and rock as well as waste containment facilities—a function generally described as a geomembrane cover. This paper presents a case history involving a novel implementation of a geomembrane cover system. Due to this novelty, the design engineers needed to assemble from disparate sources the design criteria for the engineering of the cover. This paper discusses the design methodologies assembled by the engineering team. This information will aid engineers designing similar cover systems as well as environmental and public health professionals selecting site improvements that involve infiltration barriers. PMID:21776229

  9. Dining Room Service/Setting Table Covers. Learning Activity Pack and Instructor's Guide 8.1a. Commercial Foods and Culinary Arts Competency-Based Series. Section 8: Related Services.

    ERIC Educational Resources Information Center

    Florida State Univ., Tallahassee. Center for Studies in Vocational Education.

    This document consists of a learning activity packet (LAP) for the student and an instructor's guide for the teacher. The LAP is intended to acquaint occupational home economics students with table appointments and to illustrate table covers for breakfast, luncheon, and dinner; for counter and cafeteria service; and for banquets and buffets.…

  10. Activity of a long-acting echinocandin, CD101, determined using CLSI and EUCAST reference methods, against Candida and Aspergillus spp., including echinocandin- and azole-resistant isolates

    PubMed Central

    Pfaller, Michael A.; Messer, Shawn A.; Rhomberg, Paul R.; Jones, Ronald N.; Castanheira, Mariana

    2016-01-01

    Objectives The objective of this study was to evaluate the in vitro activity of CD101, a novel echinocandin with a long serum elimination half-life, and comparator (anidulafungin and caspofungin) antifungal agents against a collection of Candida and Aspergillus spp. isolates. Methods CD101 and comparator agents were tested against 106 Candida spp. and 67 Aspergillus spp. isolates, including 27 isolates of Candida harbouring fks hotspot mutations and 12 itraconazole non-WT Aspergillus, using CLSI and EUCAST reference susceptibility broth microdilution (BMD) methods. Results Against WT and fks mutant Candida albicans, Candida glabrata and Candida tropicalis, the activity of CD101 [MIC90 = 0.06, 0.12 and 0.03 mg/L, respectively (CLSI method values)] was comparable to that of anidulafungin (MIC90 = 0.03, 0.12 and 0.03 mg/L, respectively) and caspofungin (MIC90 = 0.12, 0.25 and 0.12 mg/L, respectively). WT Candida krusei isolates were very susceptible to CD101 (MIC = 0.06 mg/L). CD101 activity (MIC50/90 = 1/2 mg/L) was comparable to that of anidulafungin (MIC50/90 = 2/2 mg/L) against Candida parapsilosis. CD101 (MIC mode = 0.06 mg/L for C. glabrata) was 2- to 4-fold more active against fks hotspot mutants than caspofungin (MIC mode = 0.5 mg/L). CD101 was active against Aspergillus fumigatus, Aspergillus terreus, Aspergillus niger and Aspergillus flavus (MEC90 range = ≤0.008–0.03 mg/L). The essential agreement between CLSI and EUCAST methods for CD101 was 92.0%–100.0% among Candida spp. and 95.0%–100.0% among Aspergillus spp. Conclusions The activity of CD101 is comparable to that of other members of the echinocandin class for the prevention and treatment of serious fungal infections. Similar results for CD101 activity versus Candida and Aspergillus spp. may be obtained with either CLSI or EUCAST BMD methods. PMID:27287236

  11. The Great Cover-Up

    ERIC Educational Resources Information Center

    Jones, Leigh Ann

    2007-01-01

    In the spring of 2005, the author created a short, online questionnaire to capture data about middle schoolers and book covers, and after several pilot studies she posted it to SurveyMonkey.com. The study includes the fiction books for middle school from the 2005 Quick Picks list. Over the course of a week, participants reported to the school…

  12. Stages of change for physical activity and dietary habits in persons with type 2 diabetes included in a mobile health intervention: the Norwegian study in RENEWING HEALTH

    PubMed Central

    Holmen, Heidi; Wahl, Astrid; Torbjørnsen, Astrid; Jenum, Anne Karen; Småstuen, Milada Cvancarova; Ribu, Lis

    2016-01-01

    Objective The aim of this study was to investigate stages of change for physical activity and dietary habits using baseline data from persons with type 2 diabetes included in a mobile health intervention. We examined the associations between stages of change for physical activity change and dietary change, and between stages of change for each behavior and individual characteristics, health-related quality of life, self-management, depressive symptoms, and lifestyle. Research design and methods We examined 151 persons with type 2 diabetes with an glycated hemoglobin (HbA1c) level ≥7.1%, aged ≥18 years at baseline of a randomized controlled trial, before testing a mobile app with or without health counseling. Stages of change were dichotomized into ‘pre-action’ and ‘action’. Self-management was measured using the Health Education Impact Questionnaire (heiQ) where a higher score reflects increased self-management, and health-related quality of life was measured with the Short-Form-36 (SF-36). Logistic regression modeling was performed. Results The median HbA1c level was 7.9% (7.1–12.4), 90% were overweight or obese, and 20% had ≥3 comorbidities. 58% were in the preaction stage for physical activity change and 79% in the preaction stage for dietary change. Higher scores of self-management were associated with an increased chance of being in the action stage for both dietary change and physical activity change. Higher body mass index was associated with an 8% reduced chance of being in the action stage for physical activity change (OR 0.92, 95% CI 0.86 to 0.99). Conclusions Being in the action stage was associated with higher scores of self-management, crucial for type 2 diabetes. Over half of the participants were in the preaction stage for physical activity and dietary change, and many had a high disease burden with comorbidities and overweight. Trial registration number NCT01315756. PMID:27239317

  13. Human Driving Forces and Their Impacts on Land Use/Land Cover. Hands-On! Developing Active Learning Modules on the Human Dimensions of Global Change.

    ERIC Educational Resources Information Center

    Moser, Susanne

    This learning module aims to engage students in problem solving, critical thinking, scientific inquiry, and cooperative learning. The module is appropriate for use in any introductory or intermediate undergraduate course that focuses on human-environment relationships. The module explains that land use/cover change has occurred at all times in all…

  14. On the Cover

    ERIC Educational Resources Information Center

    Hays, Kate F.

    2006-01-01

    This is a discussion with Judith Warren regarding her painting on the cover of the present issue of American Psychologist. To Warren, the painting on the cover of this issue, Pentimento, speaks to the interplay of spontaneity and intentionality in psychotherapy.

  15. Silostop Bunker Covers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The quality of the seal provided by the plastic cover is a key issue for minimizing losses in bunker and pile silos. Most bunker covers are 6 to 8 mil polyethylene sheets held in place by tires or tire sidewalls. Frequently there are problems with spoilage at the shoulders (i.e., against the walls),...

  16. A Comparison of Brunt Criteria, the Non Alcoholic Fatty Liver Disease Activity Score (NAS) & a Proposed NAS-including fibrosis as Valid Diagnostic Scores for NASH

    PubMed Central

    Santiago-Rolón, Amarilys; Purcell, Dagmary; Rosado, Kathia; Toro, Doris H.

    2016-01-01

    Objective Non-alcoholic steatohepatitis (NASH) can result in cirrhosis and end stage liver disease. It is of utmost importance to differentiate NASH from simple steatosis. The aim of this study is to determine the prevalence of NASH in Latino veterans with metabolic syndrome and compare histologic grading using Brunt Criteria, the NAFLD activity score (NAS), and a proposed NAS score including fibrosis. Methods Veterans with metabolic syndrome, hepatic steatosis and elevation of ALT/AST who underwent a liver biopsy from 2004-2010 were included in this study. Biopsies were evaluated by a single blinded Hepatopathologist. Steatosis, lobular inflammation, ballooning and fibrosis were graded per specimen. Each biopsy was evaluated using Brunt criteria, NAS and NAS plus fibrosis. Results Sixty patients were included in this study, 88.3% men with a mean age of 50.4 (± 12.8). 50.0% met criteria for NASH according to the Brunt system. When classifying biopsies using NAS, only 30.0% (18/60) had a score ≥5, while when adding fibrosis, the number of patients with a score ≥5 increased to 33 (55.0%). When evaluating the predictive ability of the two scoring systems, we found that NAS including fibrosis had a higher sensitivity than NAS (86.7% vs. 40.0%) and a lower specificity (76.7% vs. 80.0%). Conclusion In our population with metabolic syndrome and altered liver function tests, about 50-55% had steatohepatitis. There were significant differences between the scoring systems. When using NAS-plus-fibrosis more patients were recognized and the sensitivity increased. Further validation studies are required to evaluate this proposed NAS scoring System. PMID:26602577

  17. Land Cover Trends Project

    USGS Publications Warehouse

    Acevedo, William

    2006-01-01

    The Land Cover Trends Project is designed to document the types, rates, causes, and consequences of land cover change from 1973 to 2000 within each of the 84 U.S. Environmental Protection Agency (EPA) Level III ecoregions that span the conterminous United States. The project's objectives are to: * Develop a comprehensive methodology using probability sampling and change analysis techniques and Landsat Multispectral Scanner (MSS), Thematic Mapper (TM), and Enhanced Thematic Mapper (ETM) data for estimating regional land cover change. * Characterize the spatial and temporal characteristics of conterminous U.S. land cover change for five periods from 1973 to 2000 (nominally 1973, 1980, 1986, 1992, and 2000). * Document the regional driving forces and consequences of change. * Prepare a national synthesis of land cover change.

  18. Consequences of land use and land cover change

    USGS Publications Warehouse

    Slonecker, E. Terrence; Barnes, Christopher; Karstensen, Krista; Milheim, Lesley E.; Roig-Silva, Coral M.

    2013-01-01

    The U.S. Geological Survey (USGS) Climate and Land Use Change Mission Area is one of seven USGS mission areas that focuses on making substantial scientific "...contributions to understanding how Earth systems interact, respond to, and cause global change". Using satellite and other remotely sensed data, USGS scientists monitor patterns of land cover change over space and time at regional, national, and global scales. These data are analyzed to understand the causes and consequences of changing land cover, such as economic impacts, effects on water quality and availability, the spread of invasive species, habitats and biodiversity, carbon fluctuations, and climate variability. USGS scientists are among the leaders in the study of land cover, which is a term that generally refers to the vegetation and artificial structures that cover the land surface. Examples of land cover include forests, grasslands, wetlands, water, crops, and buildings. Land use involves human activities that take place on the land. For example, "grass" is a land cover, whereas pasture and recreational parks are land uses that produce a cover of grass.

  19. PREFACE: 9th International Fröhlich's Symposium: Electrodynamic Activity of Living Cells (Including Microtubule Coherent Modes and Cancer Cell Physics)

    NASA Astrophysics Data System (ADS)

    Cifra, Michal; Pokorný, Jirí; Kucera, Ondrej

    2011-12-01

    This volume contains papers presented at the International Fröhlich's Symposium entitled 'Electrodynamic Activity of Living Cells' (1-3 July 2011, Prague, Czech Republic). The Symposium was the 9th meeting devoted to physical processes in living matter organized in Prague since 1987. The hypothesis of oscillation systems in living cells featured by non-linear interaction between elastic and electrical polarization fields, non-linear interactions between the system and the heat bath leading to energy downconversion along the frequency scale, energy condensation in the lowest frequency mode and creation of a coherent state was formulated by H Fröhlich, founder of the theory of dielectric materials. He assumed that biological activity is based not only on biochemical but also on biophysical mechanisms and that their disturbances form basic links along the cancer transformation pathway. Fröhlich outlined general ideas of non-linear physical processes in biological systems. The downconversion and the elastic-polarization interactions should be connected in a unified theory and the solution based on comprehensive non-linear characteristics. Biochemical and genetic research of biological systems are highly developed and have disclosed a variety of cellular and subcellular structures, chemical reactions, molecular information transfer, and genetic code sequences - including their pathological development. Nevertheless, the cancer problem is still a big challenge. Warburg's discovery of suppressed oxidative metabolism in mitochondria in cancer cells suggested the essential role of physical mechanisms (but his discovery has remained without impact on cancer research and on the study of physical properties of biological systems for a long time). Mitochondria, the power plants of the cell, have several areas of activity-oxidative energy production is connected with the formation of a strong static electric field around them, water ordering, and liberation of non

  20. Covering the State Legislature

    ERIC Educational Resources Information Center

    Hook, Stephen C.

    1975-01-01

    Describes how journalism majors at Ball State University are required to cover the annual sessions of the Indiana legislature, and discusses some of the experiences and problems that were encountered. (RB)

  1. Preheating Water In The Covers Of Solar Water Heaters

    NASA Technical Reports Server (NTRS)

    Bhandari, Pradeep

    1995-01-01

    Solar water heaters that include glass covers over absorber plates redesigned to increase efficiencies according to proposal. Redesign includes modification of single-layer glass cover into double-layer glass cover and addition of plumbing so cool water to be heated made to flow between layers of cover before entering absorber plate.

  2. U.S. Geological Survey Scientific Activities in the Exploration of Antarctica: Introduction to Antarctica (Including USGS Field Personnel: 1946-59)

    USGS Publications Warehouse

    Tony K. Meunier Edited by Williams, Richard S.; Ferrigno, Jane G.

    2007-01-01

    international) programs in biology, geology, geophysics, hydrology, and mapping. Therefore, the USGS was the obvious choice for these tasks, because it already had a professional staff of experienced mapmakers, scientists, and program managers with the foresight, dedication, and understanding of the need for accurate maps to support the science programs in Antarctica when asked to do so by the U.S. National Academy of Sciences. Public Laws 85-743 and 87-626, signed in August 1958, and in September 1962, respectively, authorized the Secretary, U.S. Department of the Interior, through the USGS, to support mapping and scientific work in Antarctica (Meunier, 1979 [2007], appendix A). Open-File Report 2006-1116 includes scanned facsimiles of postal cachets. It has become an international practice to create postal cachets to commemorate special events and projects in Antarctica. A cachet is defined as a seal or commemorative design printed or stamped on an envelope to mark a philatelic or special event. The inked impression illustrates to the scientist, historian, stamp collector, and general public the multidisciplinary science projects staffed by USGS and collaborating scientists during the field season. Since 1960, philatelic cachets have been created by team members for each USGS field season and, in most cases, these cachets depict the specific geographic areas and field season program objectives. The cachets become a convenient documentation of the people, projects, and geographic places of interest for that year. Because the cachets are representative of USGS activities, each year's cachet is included as a digital facsimile in that year's Open-File Report. In the 1980s, multiple USGS cachets were prepared each year, one for use by the winter team at Amundsen-Scott South Pole Station and the other for the project work areas of the austral summer field season programs.

  3. Land Cover Characterization Program

    USGS Publications Warehouse

    ,

    1997-01-01

    (2) identify sources, develop procedures, and organize partners to deliver data and information to meet user requirements. The LCCP builds on the heritage and success of previous USGS land use and land cover programs and projects. It will be compatible with current concepts of government operations, the changing needs of the land use and land cover data users, and the technological tools with which the data are applied.

  4. In vitro activity of ceftazidime/avibactam against Gram-negative pathogens isolated from pneumonia in hospitalised patients, including ventilated patients.

    PubMed

    Flamm, Robert K; Nichols, Wright W; Sader, Helio S; Farrell, David J; Jones, Ronald N

    2016-03-01

    The activities of the novel β-lactam/non-β-lactam β-lactamase inhibitor combination ceftazidime/avibactam and comparators were evaluated against isolates from pneumonia in hospitalised patients including ventilated patients (PHP, pneumonia not designated as VABP; VABP, pneumonia in ventilated patients). Isolates were from the European-Mediterranean region (EuM), China and the USA collected in the SENTRY Antimicrobial Surveillance Program between 2009 and 2011 inclusive. A total of 2393 organisms from PHP were from the EuM, 888 from China and 3213 from the USA; from VABP patients there were 918, 97 and 692 organisms collected, respectively. Among Enterobacteriaceae from PHP, ceftazidime/avibactam MIC90 values against Escherichia coli ranged from 0.25-0.5mg/L and Klebsiella spp. MIC90 values were 0.5mg/L in each region. Among VABP isolates, MIC90 values for ceftazidime/avibactam against E. coli were 0.25mg/L; for Klebsiella spp. from VABP patients, MIC90 values were similar to those obtained against PHP isolates. The MIC of ceftazidime/avibactam was ≤8mg/L against 92-96% of Pseudomonas aeruginosa isolated from PHP patients. Isolates of P. aeruginosa from VABP patients were of lower susceptibility to all antibacterial agents (e.g. depending on region, meropenem susceptibilities were 51.2-69.4% in contrast to 68.3-76.7% among PHP patients). However, ceftazidime/avibactam inhibited 79.2-95.4% of VABP isolates at an MIC of ≤8mg/L. Acinetobacter spp. were resistant to many agents and only rates of susceptibility to colistin were >90% across all regions both for PHP and VABP isolates. Ceftazidime/avibactam was generally active against a high proportion of isolates resistant to ceftazidime from PHP and VAPB patients.

  5. Brain activity mapping in Mecp2 mutant mice reveals functional deficits in forebrain circuits, including key nodes in the default mode network, that are reversed with ketamine treatment.

    PubMed

    Kron, Miriam; Howell, C James; Adams, Ian T; Ransbottom, Michael; Christian, Diana; Ogier, Michael; Katz, David M

    2012-10-01

    Excitatory-inhibitory imbalance has been identified within specific brain microcircuits in models of Rett syndrome (RTT) and other autism spectrum disorders (ASDs). However, macrocircuit dysfunction across the RTT brain as a whole has not been defined. To approach this issue, we mapped expression of the activity-dependent, immediate-early gene product Fos in the brains of wild-type (Wt) and methyl-CpG-binding protein 2 (Mecp2)-null (Null) mice, a model of RTT, before and after the appearance of overt symptoms (3 and 6 weeks of age, respectively). At 6 weeks, Null mice exhibit significantly less Fos labeling than Wt in limbic cortices and subcortical structures, including key nodes in the default mode network. In contrast, Null mice exhibit significantly more Fos labeling than Wt in the hindbrain, most notably in cardiorespiratory regions of the nucleus tractus solitarius (nTS). Using nTS as a model, whole-cell recordings demonstrated that increased Fos expression in Nulls at 6 weeks of age is associated with synaptic hyperexcitability, including increased frequency of spontaneous and miniature EPSCs and increased amplitude of evoked EPSCs in Nulls. No such effect of genotype on Fos or synaptic function was seen at 3 weeks. In the mutant forebrain, reduced Fos expression, as well as abnormal sensorimotor function, were reversed by the NMDA receptor antagonist ketamine. In light of recent findings that the default mode network is hypoactive in autism, our data raise the possibility that hypofunction within this meta-circuit is a shared feature of RTT and other ASDs and is reversible. PMID:23035095

  6. BOREAS AFM-12 1-km AVHRR Seasonal Land Cover Classification

    NASA Technical Reports Server (NTRS)

    Steyaert, Lou; Hall, Forrest G.; Newcomer, Jeffrey A. (Editor); Knapp, David E. (Editor); Loveland, Thomas R.; Smith, David E. (Technical Monitor)

    2000-01-01

    The Boreal Ecosystem-Atmosphere Study (BOREAS) Airborne Fluxes and Meteorology (AFM)-12 team's efforts focused on regional scale Surface Vegetation and Atmosphere (SVAT) modeling to improve parameterization of the heterogeneous BOREAS landscape for use in larger scale Global Circulation Models (GCMs). This regional land cover data set was developed as part of a multitemporal one-kilometer Advanced Very High Resolution Radiometer (AVHRR) land cover analysis approach that was used as the basis for regional land cover mapping, fire disturbance-regeneration, and multiresolution land cover scaling studies in the boreal forest ecosystem of central Canada. This land cover classification was derived by using regional field observations from ground and low-level aircraft transits to analyze spectral-temporal clusters that were derived from an unsupervised cluster analysis of monthly Normalized Difference Vegetation Index (NDVI) image composites (April-September 1992). This regional data set was developed for use by BOREAS investigators, especially those involved in simulation modeling, remote sensing algorithm development, and aircraft flux studies. Based on regional field data verification, this multitemporal one-kilometer AVHRR land cover mapping approach was effective in characterizing the biome-level land cover structure, embedded spatially heterogeneous landscape patterns, and other types of key land cover information of interest to BOREAS modelers.The land cover mosaics in this classification include: (1) wet conifer mosaic (low, medium, and high tree stand density), (2) mixed coniferous-deciduous forest (80% coniferous, codominant, and 80% deciduous), (3) recent visible bum, vegetation regeneration, or rock outcrops-bare ground-sparsely vegetated slow regeneration bum (four classes), (4) open water and grassland marshes, and (5) general agricultural land use/ grasslands (three classes). This land cover mapping approach did not detect small subpixel-scale landscape

  7. Pin-Retraction Mechanism On Quick-Release Cover

    NASA Technical Reports Server (NTRS)

    Macmartin, Malcolm

    1994-01-01

    Quick-release cover includes pin-retraction mechanism releasing cover quickly from lower of two sets of pin connections holding cover. Cover released at top by pulling lever as described in "Lever-Arm Pin Puller" (NPO-18788). Removal of cover begins when technician or robot pulls upper-pin-release lever. Cover swings downward until tabs on lower pins are pulled through slots in their receptacles. Lower pins are then free.

  8. Cover times of random searches

    NASA Astrophysics Data System (ADS)

    Chupeau, Marie; Bénichou, Olivier; Voituriez, Raphaël

    2015-10-01

    How long must one undertake a random search to visit all sites of a given domain? This time, known as the cover time, is a key observable to quantify the efficiency of exhaustive searches, which require a complete exploration of an area and not only the discovery of a single target. Examples range from immune-system cells chasing pathogens to animals harvesting resources, from robotic exploration for cleaning or demining to the task of improving search algorithms. Despite its broad relevance, the cover time has remained elusive and so far explicit results have been scarce and mostly limited to regular random walks. Here we determine the full distribution of the cover time for a broad range of random search processes, including Lévy strategies, intermittent strategies, persistent random walks and random walks on complex networks, and reveal its universal features. We show that for all these examples the mean cover time can be minimized, and that the corresponding optimal strategies also minimize the mean search time for a single target, unambiguously pointing towards their robustness.

  9. 34 CFR 303.15 - Include; including.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Include; including. 303.15 Section 303.15 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION EARLY INTERVENTION PROGRAM FOR INFANTS AND TODDLERS...

  10. Kinetics of Hydrogen Radical Reactions with Toluene Including Chemical Activation Theory Employing System-Specific Quantum RRK Theory Calibrated by Variational Transition State Theory.

    PubMed

    Bao, Junwei Lucas; Zheng, Jingjing; Truhlar, Donald G

    2016-03-01

    Pressure-dependent reactions are ubiquitous in combustion and atmospheric chemistry. We employ a new calibration procedure for quantum Rice-Ramsperger-Kassel (QRRK) unimolecular rate theory within a chemical activation mechanism to calculate the pressure-falloff effect of a radical association with an aromatic ring. The new theoretical framework is applied to the reaction of H with toluene, which is a prototypical reaction in the combustion chemistry of aromatic hydrocarbons present in most fuels. Both the hydrogen abstraction reactions and the hydrogen addition reactions are calculated. Our system-specific (SS) QRRK approach is adjusted with SS parameters to agree with multistructural canonical variational transition state theory with multidimensional tunneling (MS-CVT/SCT) at the high-pressure limit. The new method avoids the need for the usual empirical estimations of the QRRK parameters, and it eliminates the need for variational transition state theory calculations as a function of energy, although in this first application we do validate the falloff curves by comparing SS-QRRK results without tunneling to multistructural microcanonical variational transition state theory (MS-μVT) rate constants without tunneling. At low temperatures, the two approaches agree well with each other, but at high temperatures, SS-QRRK tends to overestimate falloff slightly. We also show that the variational effect is important in computing the energy-resolved rate constants. Multiple-structure anharmonicity, torsional-potential anharmonicity, and high-frequency-mode vibrational anharmonicity are all included in the rate computations, and torsional anharmonicity effects on the density of states are investigated. Branching fractions, which are both temperature- and pressure-dependent (and for which only limited data is available from experiment), are predicted as a function of pressure. PMID:26841076

  11. Kinetics of Hydrogen Radical Reactions with Toluene Including Chemical Activation Theory Employing System-Specific Quantum RRK Theory Calibrated by Variational Transition State Theory.

    PubMed

    Bao, Junwei Lucas; Zheng, Jingjing; Truhlar, Donald G

    2016-03-01

    Pressure-dependent reactions are ubiquitous in combustion and atmospheric chemistry. We employ a new calibration procedure for quantum Rice-Ramsperger-Kassel (QRRK) unimolecular rate theory within a chemical activation mechanism to calculate the pressure-falloff effect of a radical association with an aromatic ring. The new theoretical framework is applied to the reaction of H with toluene, which is a prototypical reaction in the combustion chemistry of aromatic hydrocarbons present in most fuels. Both the hydrogen abstraction reactions and the hydrogen addition reactions are calculated. Our system-specific (SS) QRRK approach is adjusted with SS parameters to agree with multistructural canonical variational transition state theory with multidimensional tunneling (MS-CVT/SCT) at the high-pressure limit. The new method avoids the need for the usual empirical estimations of the QRRK parameters, and it eliminates the need for variational transition state theory calculations as a function of energy, although in this first application we do validate the falloff curves by comparing SS-QRRK results without tunneling to multistructural microcanonical variational transition state theory (MS-μVT) rate constants without tunneling. At low temperatures, the two approaches agree well with each other, but at high temperatures, SS-QRRK tends to overestimate falloff slightly. We also show that the variational effect is important in computing the energy-resolved rate constants. Multiple-structure anharmonicity, torsional-potential anharmonicity, and high-frequency-mode vibrational anharmonicity are all included in the rate computations, and torsional anharmonicity effects on the density of states are investigated. Branching fractions, which are both temperature- and pressure-dependent (and for which only limited data is available from experiment), are predicted as a function of pressure.

  12. U.S. Geological Survey Scientific Activities in the Exploration of Antarctica: Introduction to Antarctica (Including USGS Field Personnel: 1946-59)

    USGS Publications Warehouse

    Tony K. Meunier Edited by Williams, Richard S.; Ferrigno, Jane G.

    2007-01-01

    3) significant changes that have occurred in Antarctic exploration and research since World War II will be discussed at the end of this report. Subsequent Open-File Reports will provide a year-by-year documentation of USGS scientific activities and accomplishments in Antarctica beginning with the post-IGY, 1959-60 research team. One Open-File Report is planned to be written for each field-based season. For an example of the series format, see Open-File Reports 2006-1113 (Meunier, 2007a) and 2006-1114 (Meunier, 2007b). This report is a companion document to Open-File Report 2006-1116 (Meunier, 2007c). The USGS mapping and science programs in Antarctica are among the longest continuously funded projects in the United States Antarctic Program (USAP). The 2005-06 field season is the 56th consecutive U.S. expedition in which USGS scientists have been participants, starting in 1946. USGS and the National Science Foundation (NSF) cooperation began with the establishment by NSF of the U.S. Antarctic (Research) Program [USA(R)P] in 1958-59 under Operation Deep Freeze IV (DF IV) and was given the responsibility for the principal coordination and management of all U.S. scientific activities in Antarctica in Deep Freeze 60 (DF 60) (1959-60). Financial support from NSF, mostly in the form of Memorandum of Understandings (MOUs) and Cooperative Agreements, extends back to this period and can be attributed to the need for accurate geologic, geophysical, and topographic base maps of specific field areas or regions where NSF-funded science projects were planned. The epoch of Antarctic exploration during the IGY was driven by science and, in a spirit of peaceful cooperation, the international scientific community wanted to limit military activities on the continent to logistical support (Meunier, 1979 [2007], p. 38). The USGS, a Federal civilian science agency in the Department of the Interior, has, since its founding in 1879, carried out numerous field-based national (and some

  13. 42 CFR 418.202 - Covered services.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... procedures necessary for pain control or acute or chronic symptom management. Inpatient care may also be... terminal illness are covered. Appliances may include covered durable medical equipment as described in... or management of the patient's terminal illness. Equipment is provided by the hospice for use in...

  14. 42 CFR 418.202 - Covered services.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... procedures necessary for pain control or acute or chronic symptom management. Inpatient care may also be... terminal illness are covered. Appliances may include covered durable medical equipment as described in... or management of the patient's terminal illness. Equipment is provided by the hospice for use in...

  15. 42 CFR 418.202 - Covered services.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... procedures necessary for pain control or acute or chronic symptom management. Inpatient care may also be... terminal illness are covered. Appliances may include covered durable medical equipment as described in... or management of the patient's terminal illness. Equipment is provided by the hospice for use in...

  16. 42 CFR 418.202 - Covered services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... procedures necessary for pain control or acute or chronic symptom management. Inpatient care may also be... terminal illness are covered. Appliances may include covered durable medical equipment as described in... or management of the patient's terminal illness. Equipment is provided by the hospice for use in...

  17. Winter cover crops influence Amaranthus palmeri establishment

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Winter cover crops were evaluated for their effect on Palmer amaranth (PA) suppression in cotton production. Cover crops examined included rye and four winter legumes: narrow-leaf lupine, crimson clover, Austrian winter pea, and cahaba vetch. Each legume was evaluated alone and in a mixture with rye...

  18. Covering All Options

    ERIC Educational Resources Information Center

    Kennedy, Mike

    2011-01-01

    The day a school opens its doors for the first time, the flooring will be new and untarnished. When the flooring is in such pristine condition, many flooring materials--carpeting, vinyl, terrazzo, wood or some other surface--will look good. But school and university planners who decide what kind of material covers the floors of their facilities…

  19. Issue Cover (June 2016).

    PubMed

    2016-06-01

    Cover legend: Yeast cells were labeled with the fluorescent viability dyes propidium iodide (Red) and DiBAC4(3) (green) and the nucleus was stained with DAPI (blue). Cells were visualized using wide-field fluorescent microscopy. See Chadwick et al. Traffic 2016; 17(6):689-703. Read the full article on doi:10.1111/tra.12391. PMID:27174376

  20. Issue Cover (September 2016).

    PubMed

    2016-09-01

    Cover legend: Macrophages phagocytosing RFP-labeled E.coli. GFP-APPL2 labels the phagosomal membrane. Image produced by N. Condon. See Yeo et al. Traffic 2016; 17(9):1014-1026. Read the full article on doi:10.1111/tra.12415. PMID:27510703

  1. Effects of Lifestyle Interventions That Include a Physical Activity Component in Class II and III Obese Individuals: A Systematic Review and Meta-Analysis

    PubMed Central

    Baillot, Aurélie; Romain, Ahmed J.; Boisvert-Vigneault, Katherine; Audet, Mélisa; Baillargeon, Jean Patrice; Dionne, Isabelle J.; Valiquette, Louis; Chakra, Claire Nour Abou; Avignon, Antoine; Langlois, Marie-France

    2015-01-01

    Background In class II and III obese individuals, lifestyle intervention is the first step to achieve weight loss and treat obesity-related comorbidities before considering bariatric surgery. A systematic review, meta-analysis, and meta-regression were performed to assess the impact of lifestyle interventions incorporating a physical activity (PA) component on health outcomes of class II and III obese individuals. Methods An electronic search was conducted in 4 databases (Medline, Scopus, CINAHL and Sportdiscus). Two independent investigators selected original studies assessing the impact of lifestyle interventions with PA components on anthropometric parameters, cardiometabolic risk factors (fat mass, blood pressure, lipid and glucose metabolism), behaviour modification (PA and nutritional changes), and quality of life in adults with body mass index (BMI) ≥ 35 kg/m2. Estimates were pooled using a random-effect model (DerSimonian and Laird method). Heterogeneity between studies was assessed by the Cochran’s chi-square test and quantified through an estimation of the I². Results Of the 3,170 identified articles, 56 met our eligibility criteria, with a large majority of uncontrolled studies (80%). The meta-analysis based on uncontrolled studies showed significant heterogeneity among all included studies. The pooled mean difference in weight loss was 8.9 kg (95% CI, 10.2–7.7; p < 0.01) and 2.8 kg/m² in BMI loss (95% CI, 3.4–2.2; p < 0.01). Long-term interventions produced superior weight loss (11.3 kg) compared to short-term (7.2 kg) and intermediate-term (8.0 kg) interventions. A significant global effect of lifestyle intervention on fat mass, waist circumference, blood pressure, total cholesterol, LDL-C, triglycerides and fasting insulin was found (p<0.01), without significant effect on HDL-C and fasting blood glucose. Conclusions Lifestyle interventions incorporating a PA component can improve weight and various cardiometabolic risk factors in class II

  2. Sky Cover from MFRSR Observations

    SciTech Connect

    Kassianov, Evgueni I.; Barnard, James C.; Berg, Larry K.; Flynn, Connor J.; Long, Charles N.

    2011-07-01

    The diffuse all-sky surface irradiances measured at two nearby wavelengths in the visible spectral range and their model clear-sky counterparts are two main components of a new method for estimating the fractional sky cover of different cloud types, including cumulus clouds. The performance of this method is illustrated using 1-min resolution data from ground-based Multi-Filter Rotating Shadowband Radiometer (MFRSR). The MFRSR data are collected at the U.S. Department of Energy Atmospheric Radiation Measurement (ARM) Climate Research Facility (ACRF) Southern Great Plains (SGP) site during the summer of 2007 and represent 13 days with cumulus clouds. Good agreement is obtained between estimated values of the fractional sky cover and those provided by a well-established independent method based on broadband observations.

  3. Sky cover from MFRSR observations

    NASA Astrophysics Data System (ADS)

    Kassianov, E.; Barnard, J. C.; Berg, L. K.; Flynn, C.; Long, C. N.

    2011-07-01

    The diffuse all-sky surface irradiances measured at two nearby wavelengths in the visible spectral range and their modeled clear-sky counterparts are the main components of a new method for estimating the fractional sky cover of different cloud types, including cumuli. The performance of this method is illustrated using 1-min resolution data from a ground-based Multi-Filter Rotating Shadowband Radiometer (MFRSR). The MFRSR data are collected at the US Department of Energy Atmospheric Radiation Measurement (ARM) Climate Research Facility (ACRF) Southern Great Plains (SGP) site during the summer of 2007 and represent 13 days with cumuli. Good agreement is obtained between estimated values of the fractional sky cover and those provided by a well-established independent method based on broadband observations.

  4. Seed dormancy breaking diterpenoids, including novel brassicicenes J and K, from fungus Alternaria brassicicola, and their necrotic/apoptotic activities in HL-60 cells.

    PubMed

    Kenmoku, Hiromichi; Takeue, Sayaka; Oogushi, Megumi; Yagi, Yasuyuki; Sassa, Takeshi; Toyota, Masao; Asakawa, Yoshinori

    2014-03-01

    To find new metabolites similar to cotylenins and fusicoccins from the fungus Alternaria brassicicola, screening tests were carried out using the lettuce seed dormancy breaking assay. Activity-guided fractionation of the EtOAc extract from the culture using the assay afforded the isolation of two novel fusicoccane diterpenoids named brassicicenes J (1) and K (2), along with three known brassicicenes A (3), B (4), and F (5). Their structures were elucidated from extensive NMR spectral data and by comparison of these with those reported in the literature. Brassicicenes (1-5) exhibited weak to moderate seed dormancy breaking activities against lettuce seeds in the presence of abscisic acid. In addition, the necrotic/apoptotic activities of the brassicicenes (1-5), fusicoccin A (6) and cotylenin A (7) were evaluated by determining their cytotoxicity, cell viability and caspase-3/7 activation on the HL-60 cell line. Brassicicene K (2) exhibited similar cytostatic profiles to that of cotylenin A (7), and brassicicenes J (1), A (3), B (4), and F (5) exhibited necrotic activity. This is the first report of the seed dormancy breaking activity of brassicicenes in plants, and of necrotic/apoptotic activity in mammalian cells. PMID:24689212

  5. Issue Cover (July 2016).

    PubMed

    2016-07-01

    Cover legend: N-cadherin clusters colocalize with Rab5 at the macropinosomes. Confocal microscopy image of an Ncad-GFP (green) transfected COS7 cell fed with fluorescent-dextran to label macropinosomes (blue) followed by immunofluorescence staining of Rab5 (red) and the nucleus (cyan). See Wen et al. Traffic 2016; 17(7):769-785. Read the full article on doi: 10.1111/tra.12402. PMID:27297702

  6. Validation of the Japanese version of the Systemic Lupus Activity Questionnaire that includes physician-based assessments in a large observational cohort.

    PubMed

    Okamoto, Y; Katsumata, Y; Baba, S; Kawaguchi, Y; Gono, T; Hanaoka, M; Kawasumi, H; Yamanaka, H

    2016-04-01

    The Systemic Lupus Activity Questionnaire (SLAQ) is a patient-reported outcome for systemic lupus erythematosus (SLE). We aimed to translate it into Japanese and further investigate its validity and reliability. The English version of the SLAQ was translated into Japanese and administered to Japanese SLE patients at our university clinic. Physicians assessed disease activity using the SLE Disease Activity Index 2000 (SLEDAI-2K). The patients were prospectively followed for repeat assessment a year later. Ultimately, 255 patients participated. The patients' 10-point ratings of disease activity and SLAQ scores were significantly correlated (Spearman's ρ = 0.53). The SLAQ score was weakly correlated with the SLE Disease Activity Index 2000 (SLEDAI-2K)-nolab (omitting laboratory items; ρ = 0.18) but not with the SLEDAI-2K (ρ = 0.02). These results suggested its convergent and discriminant validity. The SLAQ demonstrated acceptable internal consistency (Cronbach's α = 0.80), and good test-retest reliability (intraclass correlation coefficient = 0.85). The effect sizes and the standardized response means of the SLAQ were as follows: clinical worsening, 0.26 and 0.31, and improvement, -0.39 and -0.41, respectively, which indicated a small but significant responsiveness. The Japanese version of the SLAQ demonstrated acceptable reliability and validity; its performance was comparable to that of the original version.

  7. Surface plasmon resonance enhanced visible-light-driven photocatalytic activity in Cu nanoparticles covered Cu2O microspheres for degrading organic pollutants

    NASA Astrophysics Data System (ADS)

    Cheng, Yahui; Lin, Yuanjing; Xu, Jianping; He, Jie; Wang, Tianzhao; Yu, Guojun; Shao, Dawei; Wang, Wei-Hua; Lu, Feng; Li, Lan; Du, Xiwen; Wang, Weichao; Liu, Hui; Zheng, Rongkun

    2016-03-01

    Micron-sized Cu2O with different coverage of Cu nanoparticles (NPs) on the sphere has been synthesized by a redox procedure. The absorption spectra show that Cu NPs induce the surface plasmon resonance (SPR) at the wavelength of ∼565 nm. Methylene blue (MB) photodegrading experiments under visible-light display that the Cu2O-Cu-H2O2 system exhibits a superior photocatalytic activity to Cu2O-H2O2 or pure H2O2 with an evident dependency on Cu coverage. The maximum photodegradation rate is 88% after visible-light irradiating for 60 min. The role of the Cu NPs is clarified through photodegradation experiments under 420 nm light irradiation, which is different from the SPR wavelength of Cu NPs (∼565 nm). By excluding the SPR effect, it proves that Cu SPR plays a key role in the photodegradation. Besides, a dark catalytic activity is observed stemming from the Fenton-like reaction with the aid of H2O2. The radical quenching experiments indicate that both •O2- and •OH radicals contribute to the photocatalysis, while the dark catalysis is only governed by the •OH radicals, leading to a lower activity comparing with the photocatalysis. Therefore, with introducing Cu NPs and H2O2, the Cu2O-based photocatalytic activity could be significantly improved due to the SPR effect and dark catalysis.

  8. Development of operational models of receptor activation including constitutive receptor activity and their use to determine the efficacy of the chemokine CCL17 at the CC chemokine receptor CCR4.

    PubMed

    Slack, R J; Hall, D A

    2012-07-01

    BACKGROUND AND PURPOSE The operational model provides a key conceptual framework for the analysis of pharmacological data. However, this model does not include constitutive receptor activity, a frequent phenomenon in modern pharmacology, particularly in recombinant systems. Here, we developed extensions of the operational model which include constitutive activity and applied them to effects of agonists at the chemokine receptor CCR4. EXPERIMENTAL APPROACH The effects of agonists of CCR4 on [(35) S]GTPγS binding to recombinant cell membranes and on the filamentous (F-) actin content of human CD4(+) CCR4(+) T cells were determined. The basal [(35) S]GTPγS binding was changed by varying the GDP concentration whilst the basal F-actin contents of the higher expressing T cell populations were elevated, suggesting constitutive activity of CCR4. Both sets of data were analysed using the mathematical models. RESULTS The affinity of CCL17 (also known as TARC) derived from analysis of the T cell data (pK(a) = 9.61 ± 0.17) was consistent with radioligand binding experiments (9.50 ± 0.11) while that from the [(35) S]GTPγS binding experiments was lower (8.27 ± 0.09). Its intrinsic efficacy differed between the two systems (110 in T cells vs. 11). CONCLUSIONS AND IMPLICATIONS The presence of constitutive receptor activity allows the absolute intrinsic efficacy of agonists to be determined without a contribution from the signal transduction system. Intrinsic efficacy estimated in this way is consistent with Furchgott's definition of this property. CCL17 may have a higher intrinsic efficacy at CCR4 in human T cells than that expressed recombinantly in CHO cells.

  9. On the recent seismic activity in North-Eastern Aegean Sea including the Mw5.8 earthquake on 8 January 2013

    PubMed Central

    SARLIS, Nicholas V.

    2013-01-01

    In the last week of November 2012, we announced that a strong electrotelluric disturbance, which we judged to be a Seismic Electric Signal (SES) activity, was recorded at station Assiros located in Northern Greece. This disturbance was actually followed by an Mw5.8 earthquake on 8 January 2013 in North-Eastern Aegean Sea. Here we show that, by analyzing this SES activity and employing the natural time analysis of subsequent seismicity, we estimated the epicentral location, magnitude and occurrence time which are reasonably compatible with those of the Mw5.8 event. PMID:24213207

  10. FBI-1 Can Stimulate HIV-1 Tat Activity and Is Targeted to a Novel Subnuclear Domain that Includes the Tat-P-TEFb—containing Nuclear Speckles

    PubMed Central

    Pendergrast, P. Shannon; Wang, Chen; Hernandez, Nouria; Huang, Sui

    2002-01-01

    FBI-1 is a cellular POZ-domain–containing protein that binds to the HIV-1 LTR and associates with the HIV-1 transactivator protein Tat. Here we show that elevated levels of FBI-1 specifically stimulate Tat activity and that this effect is dependent on the same domain of FBI-1 that mediates Tat-FBI-1 association in vivo. FBI-1 also partially colocalizes with Tat and Tat's cellular cofactor, P-TEFb (Cdk9 and cyclin T1), at the splicing-factor–rich nuclear speckle domain. Further, a less-soluble population of FBI-1 distributes in a novel peripheral-speckle pattern of localization as well as in other nuclear regions. This distribution pattern is dependent on the FBI-1 DNA binding domain, on the presence of cellular DNA, and on active transcription. Taken together, these results suggest that FBI-1 is a cellular factor that preferentially associates with active chromatin and that can specifically stimulate Tat-activated HIV-1 transcription. PMID:11907272

  11. FBI-1 can stimulate HIV-1 Tat activity and is targeted to a novel subnuclear domain that includes the Tat-P-TEFb-containing nuclear speckles.

    PubMed

    Pendergrast, P Shannon; Wang, Chen; Hernandez, Nouria; Huang, Sui

    2002-03-01

    FBI-1 is a cellular POZ-domain-containing protein that binds to the HIV-1 LTR and associates with the HIV-1 transactivator protein Tat. Here we show that elevated levels of FBI-1 specifically stimulate Tat activity and that this effect is dependent on the same domain of FBI-1 that mediates Tat-FBI-1 association in vivo. FBI-1 also partially colocalizes with Tat and Tat's cellular cofactor, P-TEFb (Cdk9 and cyclin T1), at the splicing-factor-rich nuclear speckle domain. Further, a less-soluble population of FBI-1 distributes in a novel peripheral-speckle pattern of localization as well as in other nuclear regions. This distribution pattern is dependent on the FBI-1 DNA binding domain, on the presence of cellular DNA, and on active transcription. Taken together, these results suggest that FBI-1 is a cellular factor that preferentially associates with active chromatin and that can specifically stimulate Tat-activated HIV-1 transcription.

  12. Relationships Between Design Characteristics of Avionics Subsystems and Training Cost, Training Difficulty, and Job Performance. Final Report, Covering Activity from 1 July 1971 Through 1 September 1972.

    ERIC Educational Resources Information Center

    Lintz, Larry M.; And Others

    A study investigated the relationship between avionics subsystem design characteristics and training time, training cost, and job performance. A list of design variables believed to affect training and job performance was established and supplemented with personnel variables, including aptitude test scores and the amount of training and…

  13. From the Cover: Tetrachlorobenzoquinone Exerts Neurological Proinflammatory Activity by Promoting HMGB1 Release, Which Induces TLR4 Clustering within the Lipid Raft.

    PubMed

    Fu, Juanli; Shi, Qiong; Song, Xiufang; Liu, Zixuan; Wang, Yawen; Wang, Yuxin; Song, Erqun; Song, Yang

    2016-10-01

    Tetrachlorobenzoquinone (TCBQ) is a confirmed active metabolite of a well-known environmental pollutant pentachlorophenol (PCP). Unfortunately, there is insufficient knowledge present available on TCBQ's toxicity. Our previous studies indicated that TCBQ induces inflammatory response in vivo and in vitro; however, its exact mechanism needs further investigation. Toll-like receptors (TLRs) play a crucial role in conveying of inflammatory signaling, whilst high-mobility group box 1 (HMGB1) functions as a transcription-enhancing nuclear protein that regulates inflammation. Indeed, this study demonstrated that TCBQ induces the secretion/translocation of HMGB1, which in turn activates its receptors, TLR family gene (especially TLR4) and receptor for advanced glycation end-products (RAGE) expressions. Consistently, the binding affinity of HMGB1 with its receptors also increased. In the case of HMGB1 or TLR4 deficiency, there were decreases in TCBQ-induced neuroinflammatory cytokine production and neuropathological changes, eg, neuronal loss, astrocyte and macrophage cells activation. Moreover, we found the mobilization of TLR4 into lipid rafts occurs in response to TCBQ exposure, lipid rafts disruptors weakened this effect, suggested lipid rafts play an essential role for TLR4-mediated signal transduction and target inflammatory cytokines expressions. In summary, our current findings revealed a previously unknown mechanism of TCBQ-induced neurological inflammation related to HMGB1-TLR4 signaling.

  14. Applying the model of Goal-Directed Behavior, including descriptive norms, to physical activity intentions: A contribution to improving the Theory of Planned Behavior

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The theory of planned behavior (TPB) has received its fair share of criticism lately, including calls for it to retire. We contributed to improving the theory by testing extensions such as the model of goal-directed behavior (MGDB, which adds desire and anticipated positive and negative emotions) ap...

  15. EspC, an Autotransporter Protein Secreted by Enteropathogenic Escherichia coli, Causes Apoptosis and Necrosis through Caspase and Calpain Activation, Including Direct Procaspase-3 Cleavage

    PubMed Central

    Serapio-Palacios, Antonio

    2016-01-01

    ABSTRACT Enteropathogenic Escherichia coli (EPEC) has the ability to antagonize host apoptosis during infection through promotion and inhibition of effectors injected by the type III secretion system (T3SS), but the total number of these effectors and the overall functional relationships between these effectors during infection are poorly understood. EspC produced by EPEC cleaves fodrin, paxillin, and focal adhesion kinase (FAK), which are also cleaved by caspases and calpains during apoptosis. Here we show the role of EspC in cell death induced by EPEC. EspC is involved in EPEC-mediated cell death and induces both apoptosis and necrosis in epithelial cells. EspC induces apoptosis through the mitochondrial apoptotic pathway by provoking (i) a decrease in the expression levels of antiapoptotic protein Bcl-2, (ii) translocation of the proapoptotic protein Bax from cytosol to mitochondria, (iii) cytochrome c release from mitochondria to the cytoplasm, (iv) loss of mitochondrial membrane potential, (v) caspase-9 activation, (vi) cleavage of procaspase-3 and (vii) an increase in caspase-3 activity, (viii) PARP proteolysis, and (ix) nuclear fragmentation and an increase in the sub-G1 population. Interestingly, EspC-induced apoptosis was triggered through a dual mechanism involving both independent and dependent functions of its EspC serine protease motif, the direct cleavage of procaspase-3 being dependent on this motif. This is the first report showing a shortcut for induction of apoptosis by the catalytic activity of an EPEC protein. Furthermore, this atypical intrinsic apoptosis appeared to induce necrosis through the activation of calpain and through the increase of intracellular calcium induced by EspC. Our data indicate that EspC plays a relevant role in cell death induced by EPEC. PMID:27329750

  16. Issue Cover (August 2016).

    PubMed

    2016-08-01

    Cover legend: Absence of the novel endolysosomal trafficking regulator WDR81 (green) induces the accumulation of tetherin (red) in enlarged early endosomes. WDR81 knock-out HeLa cells were genetically complemented with an HA-tagged WDR81 construct and imaged by confocal immunofluorescence microscopy. The original image was processed with photo editing software and overlaid with artistic effects. See Rapiteanu et al. Traffic 2016; 17(8):940-958. Read the full article on doi: 10.1111/tra.12409. PMID:27412792

  17. Monthly fractional green vegetation cover associated with land cover classes of the conterminous USA

    USGS Publications Warehouse

    Gallo, Kevin P.; Tarpley, Dan; Mitchell, Ken; Csiszar, Ivan; Owen, editors, Timothy W.; Reed, Bradley C.

    2001-01-01

    The land cover classes developed under the coordination of the International Geosphere-Biosphere Programme Data and Information System (IGBP-DIS) have been analyzed for a study area that includes the Conterminous United States and portions of Mexico and Canada. The 1-km resolution data have been analyzed to produce a gridded data set that includes within each 20-km grid cell: 1) the three most dominant land cover classes, 2) the fractional area associated with each of the three dominant classes, and 3) the fractional area covered by water. Additionally, the monthly fraction of green vegetation cover (fgreen) associated with each of the three dominant land cover classes per grid cell was derived from a 5-year climatology of 1-km resolution NOAA-AVHRR data. The variables derived in this study provide a potential improvement over the use of monthly fgreen linked to a single land cover class per model grid cell.

  18. Validation of Land Cover Maps Utilizing Astronaut Acquired Imagery

    NASA Technical Reports Server (NTRS)

    Estes, John E.; Gebelein, Jennifer

    1999-01-01

    This report is produced in accordance with the requirements outlined in the NASA Research Grant NAG9-1032 titled "Validation of Land Cover Maps Utilizing Astronaut Acquired Imagery". This grant funds the Remote Sensing Research Unit of the University of California, Santa Barbara. This document summarizes the research progress and accomplishments to date and describes current on-going research activities. Even though this grant has technically expired, in a contractual sense, work continues on this project. Therefore, this summary will include all work done through and 5 May 1999. The principal goal of this effort is to test the accuracy of a sub-regional portion of an AVHRR-based land cover product. Land cover mapped to three different classification systems, in the southwestern United States, have been subjected to two specific accuracy assessments. One assessment utilizing astronaut acquired photography, and a second assessment employing Landsat Thematic Mapper imagery, augmented in some cases, high aerial photography. Validation of these three land cover products has proceeded using a stratified sampling methodology. We believe this research will provide an important initial test of the potential use of imagery acquired from Shuttle and ultimately the International Space Station (ISS) for the operational validation of the Moderate Resolution Imaging Spectrometer (MODIS) land cover products.

  19. Isolation of estrogen-degrading bacteria from an activated sludge bioreactor treating swine waste, including a strain that converts estrone to β-estradiol.

    PubMed

    Isabelle, Martine; Villemur, Richard; Juteau, Pierre; Lépine, François

    2011-07-01

    An estrogen-degrading bacterial consortium from a swine wastewater biotreatment was enriched in the presence of low concentrations (1 mg/L) of estrone (E1), 17β-estradiol (βE2), and equol (EQO) as sole carbon sources. The consortium removed 99% ± 1% of these three estrogens in 48 h. Estrogen removal occurred even in the presence of an ammonia monooxygenase inhibitor, suggesting that nitrifiers are not involved. Five strains showing estrogen-metabolizing activity were isolated from the consortium on mineral agar medium with estrogens as sole carbon source. They are related to four genera ( Methylobacterium (strain MI6.1R), Ochrobactrum (strains MI6.1B and MI9.3), Pseudomonas (strain MI14.1), and Mycobacterium (strain MI21.2)) distributed among three classes (Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria). Depending on the culture medium, strains MI6.1B, MI9.3, MI14.1, and MI21.2 partially transform βE2 into E1, whereas Methylobacterium sp. strain MI6.1R reduces E1 into βE2 under aerobic conditions, in contrast with the usually observed conversion of βE2 into E1. Since βE2 is a more potent endocrine disruptor than E1, it means that the presence of Methylobacterium sp. strain MI6.1R (or other bacteria with the same E1-reducing activity) in a treatment could transiently increase the estrogenicity of the effluent. MI6.1R can also reduce the ketone group of 16-ketoestradiol, a hydroxylated analog of E1. All βE2 and E1 transformation activities were constitutive, and many of them are favoured in a rich medium than a medium containing no other carbon source. None of the isolated strains could degrade EQO. PMID:21770814

  20. From forest to farmland: pollen-inferred land cover change across Europe using the pseudobiomization approach.

    PubMed

    Fyfe, Ralph M; Woodbridge, Jessie; Roberts, Neil

    2015-03-01

    Maps of continental-scale land cover are utilized by a range of diverse users but whilst a range of products exist that describe present and recent land cover in Europe, there are currently no datasets that describe past variations over long time-scales. User groups with an interest in past land cover include the climate modelling community, socio-ecological historians and earth system scientists. Europe is one of the continents with the longest histories of land conversion from forest to farmland, thus understanding land cover change in this area is globally significant. This study applies the pseudobiomization method (PBM) to 982 pollen records from across Europe, taken from the European Pollen Database (EPD) to produce a first synthesis of pan-European land cover change for the period 9000 bp to present, in contiguous 200 year time intervals. The PBM transforms pollen proportions from each site to one of eight land cover classes (LCCs) that are directly comparable to the CORINE land cover classification. The proportion of LCCs represented in each time window provides a spatially aggregated record of land cover change for temperate and northern Europe, and for a series of case study regions (western France, the western Alps, and the Czech Republic and Slovakia). At the European scale, the impact of Neolithic food producing economies appear to be detectable from 6000 bp through reduction in broad-leaf forests resulting from human land use activities such as forest clearance. Total forest cover at a pan-European scale moved outside the range of previous background variability from 4000 bp onwards. From 2200 bp land cover change intensified, and the broad pattern of land cover for preindustrial Europe was established by 1000 bp. Recognizing the timing of anthropogenic land cover change in Europe will further the understanding of land cover-climate interactions, and the origins of the modern cultural landscape. PMID:25345850

  1. From forest to farmland: pollen-inferred land cover change across Europe using the pseudobiomization approach.

    PubMed

    Fyfe, Ralph M; Woodbridge, Jessie; Roberts, Neil

    2015-03-01

    Maps of continental-scale land cover are utilized by a range of diverse users but whilst a range of products exist that describe present and recent land cover in Europe, there are currently no datasets that describe past variations over long time-scales. User groups with an interest in past land cover include the climate modelling community, socio-ecological historians and earth system scientists. Europe is one of the continents with the longest histories of land conversion from forest to farmland, thus understanding land cover change in this area is globally significant. This study applies the pseudobiomization method (PBM) to 982 pollen records from across Europe, taken from the European Pollen Database (EPD) to produce a first synthesis of pan-European land cover change for the period 9000 bp to present, in contiguous 200 year time intervals. The PBM transforms pollen proportions from each site to one of eight land cover classes (LCCs) that are directly comparable to the CORINE land cover classification. The proportion of LCCs represented in each time window provides a spatially aggregated record of land cover change for temperate and northern Europe, and for a series of case study regions (western France, the western Alps, and the Czech Republic and Slovakia). At the European scale, the impact of Neolithic food producing economies appear to be detectable from 6000 bp through reduction in broad-leaf forests resulting from human land use activities such as forest clearance. Total forest cover at a pan-European scale moved outside the range of previous background variability from 4000 bp onwards. From 2200 bp land cover change intensified, and the broad pattern of land cover for preindustrial Europe was established by 1000 bp. Recognizing the timing of anthropogenic land cover change in Europe will further the understanding of land cover-climate interactions, and the origins of the modern cultural landscape.

  2. Hatch Cover Slides Through Hatch

    NASA Technical Reports Server (NTRS)

    Alton, Charles; Okane, James H.

    1989-01-01

    Hatch cover for pressurized vessel provides tight seal but opened quickly from either side. In opening or closing, cover sweeps out relatively little volume within vessel, so it does not hinder movement of people or objects from vessel to outside or placement of people or objects near hatch. Cover uses internal pressure to create seal when closed. Design of cover eliminates leakage paths, and cover immune to hazards of sudden decompression or jamming when bolts and latches fail.

  3. Flow cytometric analysis of the Rh1 (Rho, D) antigen activity on red cells: various Rh blood group phenotypes including Du variants.

    PubMed

    Ota, M; Hasekura, H; Fukushima, H; Yonemura, I

    1989-04-01

    Rh1 (Rho, D) antigen activity has been analyzed by the use of the indirect immunofluorescence flow cytometry (FCM), and the Rh blood group genotypes were able to be successfully determined from the intensity of fluorescence detected in flow cytometry using the anti-D IgG that was fractionated in a Protein A Sepharose CL-4B column as the primary antibody. The relative amount of the fluorescein isothiocyanate (FITC) bound to the D (R1R1, CDe/CDe), the high grade Du (R2r',cDE/Cde), the low grade Du (K1r, CDue/cde), and the d (rr, cde/cde) red cells was estimated from the mean fluorescent intensity. The FITC-binding activity of the high grade Du and low grade Du was 83% and 21% that of D. The antigen-antibody complex density profile was analyzed by using the FITC-conjugated protein-A in place of the second antibody. Compared with the found results using anti-human globulin as the second antibody, this method was less sensitive but it still was able to demonstrate the different degrees of fluorescence according to the Rh genotypes. The present FCM method is both simple and useful for (1) measuring the relative amount of antigens, (2) for detecting the dosage effect and (3) for deferminins the blood group genotypes.

  4. Fumigant toxicity of Oriental sweetgum (Liquidambar orientalis) and valerian (Valeriana wallichii) essential oils and their components, including their acetylcholinesterase inhibitory activity, against Japanese termites (Reticulitermes speratus).

    PubMed

    Park, Il-Kwon

    2014-01-01

    This study investigated the fumigant toxicity of oriental sweetgum (Liquidambar orientalis) and valerian (Valeriana wallichii) essential oils and their components against the Japanese termite (Reticulitermes speratus). The fumigant toxicity of oriental sweetgum and valerian oil differed significantly according to exposure time. Oriental sweetgum showed toxicity at short exposure times (2 days), and the toxicity of valerian oil was high 7 days after treatment. The main constituents of oriental sweetgum and valerian oils were tested individually for their fumigant toxicity against Japanese termites. Among the test compounds, benzyl alcohol, acetophenone, 1-phenyl-1-ethanol, hydrocinnamyl alcohol, trans-cinnamyl aldehyde, trans-cinnamyl alcohol, cis-asarone, styrene, and cis-ocimene showed toxicity against Japanese termites 7 days after treatment. Hydrocinnamyl alcohol and trans-cinnamyl alcohol were found to be the major contributors to the fumigant antitermitic toxicity of oriental sweetgum oil. The acetylcholinesterase (AChE) inhibition activity of two oils and their constituents was tested to determine their mode of action. Only cis-ocimene showed strong AChE inhibition activity with an IC50 value of 0.131 mg/mL. Further studies are warranted to determine the potential of these essential oils and their constituents as fumigants for termite control. PMID:25153870

  5. Fumigant toxicity of Oriental sweetgum (Liquidambar orientalis) and valerian (Valeriana wallichii) essential oils and their components, including their acetylcholinesterase inhibitory activity, against Japanese termites (Reticulitermes speratus).

    PubMed

    Park, Il-Kwon

    2014-08-19

    This study investigated the fumigant toxicity of oriental sweetgum (Liquidambar orientalis) and valerian (Valeriana wallichii) essential oils and their components against the Japanese termite (Reticulitermes speratus). The fumigant toxicity of oriental sweetgum and valerian oil differed significantly according to exposure time. Oriental sweetgum showed toxicity at short exposure times (2 days), and the toxicity of valerian oil was high 7 days after treatment. The main constituents of oriental sweetgum and valerian oils were tested individually for their fumigant toxicity against Japanese termites. Among the test compounds, benzyl alcohol, acetophenone, 1-phenyl-1-ethanol, hydrocinnamyl alcohol, trans-cinnamyl aldehyde, trans-cinnamyl alcohol, cis-asarone, styrene, and cis-ocimene showed toxicity against Japanese termites 7 days after treatment. Hydrocinnamyl alcohol and trans-cinnamyl alcohol were found to be the major contributors to the fumigant antitermitic toxicity of oriental sweetgum oil. The acetylcholinesterase (AChE) inhibition activity of two oils and their constituents was tested to determine their mode of action. Only cis-ocimene showed strong AChE inhibition activity with an IC50 value of 0.131 mg/mL. Further studies are warranted to determine the potential of these essential oils and their constituents as fumigants for termite control.

  6. Suppression of the ELO-2 FA elongation activity results in alterations of the fatty acid composition and multiple physiological defects, including abnormal ultradian rhythms, in Caenorhabditis elegans.

    PubMed Central

    Kniazeva, Marina; Sieber, Matt; McCauley, Scott; Zhang, Kang; Watts, Jennifer L; Han, Min

    2003-01-01

    While the general steps of fatty acid (FA) biosynthesis are well understood, the individual enzymes involved in the elongation of long chain saturated and polyunsaturated FA (PUFA) are largely unknown. Recent research indicates that these enzymes might be of considerable physiological importance for human health. We use Caenorhabditis elegans to study FA elongation activities and associated abnormal phenotypes. In this article we report that the predicted C. elegans F11E6.5/ELO-2 is a functional enzyme with the FA elongation activity. It is responsible for the elongation of palmitic acid and is involved in PUFA biosynthesis. RNAi-mediated suppression of ELO-2 causes an accumulation of palmitate and an associated decrease in the PUFA fraction in triacylglycerides and phospholipid classes. This imbalance in the FA composition results in multiple phenotypic defects such as slow growth, small body size, reproductive defects, and changes in rhythmic behavior. ELO-2 cooperates with the previously reported ELO-1 in 20-carbon PUFA production, and at least one of the enzymes must function to provide normal growth and development in C. elegans. The presented data indicate that suppression of a single enzyme of the FA elongation machinery is enough to affect various organs and systems in worms. This effect resembles syndromic disorders in humans. PMID:12586704

  7. Pig skin includes dendritic cell subsets transcriptomically related to human CD1a and CD14 dendritic cells presenting different migrating behaviors and T cell activation capacities.

    PubMed

    Marquet, Florian; Vu Manh, Thien-Phong; Maisonnasse, Pauline; Elhmouzi-Younes, Jamila; Urien, Céline; Bouguyon, Edwige; Jouneau, Luc; Bourge, Mickael; Simon, Gaëlle; Ezquerra, Angel; Lecardonnel, Jérôme; Bonneau, Michel; Dalod, Marc; Schwartz-Cornil, Isabelle; Bertho, Nicolas

    2014-12-15

    Swine skin is one of the best structural models for human skin, widely used to probe drug transcutaneous passage and to test new skin vaccination devices. However, little is known about its composition in immune cells, and among them dendritic cells (DC), that are essential in the initiation of the immune response. After a first seminal work describing four different DC subpopulations in pig skin, we hereafter deepen the characterization of these cells, showing the similarities between swine DC subsets and their human counterparts. Using comparative transcriptomic study, classical phenotyping as well as in vivo and in vitro functional studies, we show that swine CD163(pos) dermal DC (DDC) are transcriptomically similar to the human CD14(pos) DDC. CD163(pos) DDC are recruited in inflamed skin, they migrate in inflamed lymph but they are not attracted toward CCL21, and they modestly activate allogeneic CD8 T cells. We also show that CD163(low) DDC are transcriptomically similar to the human CD1a(pos) DDC. CD163(low) DDC migrate toward CCL21, they activate allogeneic CD8 and CD4 T cells and, like their potential human lung counterpart, they skew CD4 T cells toward a Th17 profile. We thus conclude that swine skin is a relevant model for human skin vaccination.

  8. Flow cytometric analysis of the Rh1 (Rho, D) antigen activity on red cells: various Rh blood group phenotypes including Du variants.

    PubMed

    Ota, M; Hasekura, H; Fukushima, H; Yonemura, I

    1989-04-01

    Rh1 (Rho, D) antigen activity has been analyzed by the use of the indirect immunofluorescence flow cytometry (FCM), and the Rh blood group genotypes were able to be successfully determined from the intensity of fluorescence detected in flow cytometry using the anti-D IgG that was fractionated in a Protein A Sepharose CL-4B column as the primary antibody. The relative amount of the fluorescein isothiocyanate (FITC) bound to the D (R1R1, CDe/CDe), the high grade Du (R2r',cDE/Cde), the low grade Du (K1r, CDue/cde), and the d (rr, cde/cde) red cells was estimated from the mean fluorescent intensity. The FITC-binding activity of the high grade Du and low grade Du was 83% and 21% that of D. The antigen-antibody complex density profile was analyzed by using the FITC-conjugated protein-A in place of the second antibody. Compared with the found results using anti-human globulin as the second antibody, this method was less sensitive but it still was able to demonstrate the different degrees of fluorescence according to the Rh genotypes. The present FCM method is both simple and useful for (1) measuring the relative amount of antigens, (2) for detecting the dosage effect and (3) for deferminins the blood group genotypes. PMID:2509769

  9. Heterogeneity in Pseudomonas aeruginosa Biofilms Includes Expression of Ribosome Hibernation Factors in the Antibiotic-Tolerant Subpopulation and Hypoxia-Induced Stress Response in the Metabolically Active Population

    PubMed Central

    Williamson, Kerry S.; Richards, Lee A.; Perez-Osorio, Ailyn C.; Pitts, Betsey; McInnerney, Kathleen; Stewart, Philip S.

    2012-01-01

    Bacteria growing in biofilms are physiologically heterogeneous, due in part to their adaptation to local environmental conditions. Here, we characterized the local transcriptome responses of Pseudomonas aeruginosa growing in biofilms by using a microarray analysis of isolated biofilm subpopulations. The results demonstrated that cells at the top of the biofilms had high mRNA abundances for genes involved in general metabolic functions, while mRNA levels for these housekeeping genes were low in cells at the bottom of the biofilms. Selective green fluorescent protein (GFP) labeling showed that cells at the top of the biofilm were actively dividing. However, the dividing cells had high mRNA levels for genes regulated by the hypoxia-induced regulator Anr. Slow-growing cells deep in the biofilms had little expression of Anr-regulated genes and may have experienced long-term anoxia. Transcripts for ribosomal proteins were associated primarily with the metabolically active cell fraction, while ribosomal RNAs were abundant throughout the biofilms, indicating that ribosomes are stably maintained even in slowly growing cells. Consistent with these results was the identification of mRNAs for ribosome hibernation factors (the rmf and PA4463 genes) at the bottom of the biofilms. The dormant biofilm cells of a P. aeruginosa Δrmf strain had decreased membrane integrity, as shown by propidium iodide staining. Using selective GFP labeling and cell sorting, we show that the dividing cells are more susceptible to killing by tobramycin and ciprofloxacin. The results demonstrate that in thick P. aeruginosa biofilms, cells are physiologically distinct spatially, with cells deep in the biofilm in a viable but antibiotic-tolerant slow-growth state. PMID:22343293

  10. The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells

    SciTech Connect

    Gao Jiayu; Morgan, Winston A.; Sanchez-Medina, Alberto; Corcoran, Olivia

    2011-08-01

    Despite a lack of scientific authentication, Scutellaria baicalensis is clinically used in Chinese medicine as a traditional adjuvant to chemotherapy of lung cancer. In this study, cytotoxicity assays demonstrated that crude ethanolic extracts of S. baicalensis were selectively toxic to human lung cancer cell lines A549, SK-LU-1 and SK-MES-1 compared with normal human lung fibroblasts. The active compounds baicalin, baicalein and wogonin did not exhibit such selectivity. Following exposure to the crude extracts, cellular protein expression in the cancer cell lines was assessed using 2D gel electrophoresis coupled with MALDI-TOF-MS/Protein Fingerprinting. The altered protein expression indicated that cell growth arrest and apoptosis were potential mechanisms of cytotoxicity. These observations were supported by PI staining cell cycle analysis using flow cytometry and Annexin-V apoptotic analysis by fluorescence microscopy of cancer cells treated with the crude extract and pure active compounds. Moreover, specific immunoblotting identification showed the decreased expression of cyclin A results in the S phase arrest of A549 whereas the G{sub 0}/G{sub 1} phase arrest in SK-MES-1 cells results from the decreased expression of cyclin D1. Following treatment, increased expression in the cancer cells of key proteins related to the enhancement of apoptosis was observed for p53 and Bax. These results provide further insight into the molecular mechanisms underlying the clinical use of this herb as an adjuvant to lung cancer therapy. - Research Highlights: > Scutellaria baicalensis is a clinical adjuvant to lung cancer chemotherapy in China. > Scutellaria ethanol extracts selectively toxic to A549, SK-LU-1 and SK-MES-1. > Baicalin, baicalein and wogonin were toxic to all lung cancer cell lines. > Proteomics identified increased p53 and BAX in response to Scutellaria extracts.

  11. Development of integral covers on solar cells

    NASA Technical Reports Server (NTRS)

    Stella, P.; Somberg, H.

    1971-01-01

    The electron-beam technique for evaporating a dielectric material onto solar cells is investigated. A process has been developed which will provide a highly transparent, low stress, 2 mil thick cover capable of withstanding conventional space type qualification tests including humidity, thermal shock, and thermal cycling. The covers have demonstrated the ability to withstand 10 to the 15th power 1 MeV electrons and UV irradiation with minor darkening. Investigation of the cell AR coating has produced a space qualifiable titanium oxide coating which will give an additional 6% current output over similar silicon oxide coated cells when covered by glass.

  12. MifM Monitors Total YidC Activities of Bacillus subtilis, Including That of YidC2, the Target of Regulation

    PubMed Central

    Ito, Koreaki

    2014-01-01

    The YidC/Oxa1/Alb3 family proteins are involved in membrane protein biogenesis in bacteria, mitochondria, and chloroplasts. Recent studies show that YidC uses a channel-independent mechanism to insert a class of membrane proteins into the membrane. Bacillus subtilis has two YidC homologs, SpoIIIJ (YidC1) and YidC2 (YqjG); the former is expressed constitutively, while the latter is induced when the SpoIIIJ activity is compromised. MifM is a substrate of SpoIIIJ, and its failure in membrane insertion is accompanied by stable ribosome stalling on the mifM-yidC2 mRNA, which ultimately facilitates yidC2 translation. While mutational inactivation of SpoIIIJ has been known to induce yidC2 expression, here, we show that the level of this induction is lower than that observed when the membrane insertion signal of MifM is defective. Moreover, this partial induction of YidC2 translation is lowered further when YidC2 is overexpressed in trans. These results suggest that YidC2 is able to insert MifM into the membrane and to release its translation arrest. Thus, under SpoIIIJ-deficient conditions, YidC2 expression is subject to MifM-mediated autogenous feedback repression. Our results show that YidC2 uses a mechanism that is virtually identical to that used by SpoIIIJ; Arg75 of YidC2 in its intramembrane yet hydrophilic cavity is functionally indispensable and requires negatively charged residues of MifM as an insertion substrate. From these results, we conclude that MifM monitors the total activities of the SpoIIIJ and the YidC2 pathways to control the synthesis of YidC2 and to maintain the cellular capability of the YidC mode of membrane protein biogenesis. PMID:25313395

  13. ISEE 3 observations during the CDAW 8 intervals: Case studies of the distant geomagnetic tail covering a wide range of geomagnetic activity

    SciTech Connect

    Richardson, I.G.; Owen, C.J.; Cowley, S.W.H. ); Galvin, A.B. ); Sanderson, T.R. ); Scholer, M. ); Slavin, J.A. ); Zwickl, R.D. )

    1989-11-01

    The data obtained by the ISEE 3 spacecraft during the eight Coordinated Data Analysis Workshop 8 (CDAW 8) intervals provide an excellent opportunity to study the structure and dynamics of the distant geomagnetic tail under a wide range of geomagnetic activity ranging from intervals of magnetic quiet punctuated by isolated substorms to extended intervals of strong disturbance. By examining the properties of the plasma sheet, evidence has been found for the persistence of reconnection in the tail during long intervals of magnetic quiet, with the neutral line lying {approx}100 to 200 R{sub E} or more downtail. The suggestion that the distant tail plasma sheet is populated exclusively by tailward moving closed flux tubes under quiet geomagnetic conditions is therefore not supported. However, a slow plasma sheet regime is also found during such conditions, in which closed flux tubes move slowly tailward in a thick region adjacent to the magnetopause, presumably due to some form of viscous momentum transfer from the magnetosheath. This process does not appear to simultaneously transfer mass into the tail, and there is some indication that the Kelvin-Helmholtz instability is involved. The observations strongly suggest that the closed flux tubes originate from the closed field line plasma sheet region earthward of the neutral line rather than, for example, from the near-Earth low-latitude boundary layer. Plasmoids are observed in the distant tail following disturbance enhancements, the time of their appearance being generally consistent with disconnection from the near-Earth region at the time of the enhancement. Their structure is entirely consistent with the neutral line model.

  14. Hematopoietic stem cell transplantation activity worldwide in 2012 and a SWOT analysis of the Worldwide Network for Blood and Marrow Transplantation Group including the global survey.

    PubMed

    Niederwieser, D; Baldomero, H; Szer, J; Gratwohl, M; Aljurf, M; Atsuta, Y; Bouzas, L F; Confer, D; Greinix, H; Horowitz, M; Iida, M; Lipton, J; Mohty, M; Novitzky, N; Nunez, J; Passweg, J; Pasquini, M C; Kodera, Y; Apperley, J; Seber, A; Gratwohl, A

    2016-06-01

    Data on 68 146 hematopoietic stem cell transplants (HSCTs) (53% autologous and 47% allogeneic) gathered by 1566 teams from 77 countries and reported through their regional transplant organizations were analyzed by main indication, donor type and stem cell source for the year 2012. With transplant rates ranging from 0.1 to 1001 per 10 million inhabitants, more HSCTs were registered from unrelated 16 433 donors than related 15 493 donors. Grafts were collected from peripheral blood (66%), bone marrow (24%; mainly non-malignant disorders) and cord blood (10%). Compared with 2006, an increase of 46% total (57% allogeneic and 38% autologous) was observed. Growth was due to an increase in reporting teams (18%) and median transplant activity/team (from 38 to 48 HSCTs/team). An increase of 167% was noted in mismatched/haploidentical family HSCT. A Strengths, Weaknesses, Opportunities, Threats (SWOT) analysis revealed the global perspective of WBMT to be its major strength and identified potential to be the key professional body for patients and authorities. The limited data collection remains its major weakness and threat. In conclusion, global HSCT grows over the years without plateauing (allogeneic>autologous) and at different rates in the four World Health Organization regions. Major increases were observed in allogeneic, haploidentical HSCT and, to a lesser extent, in cord blood transplantation. PMID:26901703

  15. Building Astronomy Curriculum to Include the Sight Impaired: Week long summer camp activities for Middle School Students adherent to Washington State Curriculum Standards (EALR's)

    NASA Astrophysics Data System (ADS)

    Ramien, Natalie; Loebman, S. R.; Player, V.; Larson, A.; Torcolini, N. B.; Traverse, A.

    2011-01-01

    Currently astronomy learning is heavily geared towards visual aids; however, roughly 10 million people in North America are sight impaired. Every student should have access to meaningful astronomy curriculum; an understanding of astronomy is an expectation of national and state science learning requirements. Over the last ten years, Noreen Grice has developed Braille and large print astronomy text books aimed at sight impaired learners. We build upon Grice's written work and present here a five day lesson plan that integrates 2D reading with 3D activities. Through this curriculum, students develop an intuitive understanding of astronomical distance, size, composition and lifetimes. We present five distinct lesson modules that can be taught individually or in a sequential form: the planets, our sun, stars, stellar evolution and galaxies. We have tested these modules on sight impaired students and report the results here. Overall, we find the work presented here lends itself equally well to a week long science camp geared toward middle school sight impaired taught by astronomers or as supplemental material integrated into a regular classroom science curriculum. This work was made possible by a 2007 Simple Effective Education and Dissemination (SEED) Grant For Astronomy Researchers, Astronomical Society of the Pacific through funds provided by the Planck Mission, Jet Propulsion Laboratory, California Institute of Technology.

  16. Hematopoietic stem cell transplantation activity worldwide in 2012 and a SWOT analysis of the Worldwide Network for Blood and Marrow Transplantation Group including the global survey.

    PubMed

    Niederwieser, D; Baldomero, H; Szer, J; Gratwohl, M; Aljurf, M; Atsuta, Y; Bouzas, L F; Confer, D; Greinix, H; Horowitz, M; Iida, M; Lipton, J; Mohty, M; Novitzky, N; Nunez, J; Passweg, J; Pasquini, M C; Kodera, Y; Apperley, J; Seber, A; Gratwohl, A

    2016-06-01

    Data on 68 146 hematopoietic stem cell transplants (HSCTs) (53% autologous and 47% allogeneic) gathered by 1566 teams from 77 countries and reported through their regional transplant organizations were analyzed by main indication, donor type and stem cell source for the year 2012. With transplant rates ranging from 0.1 to 1001 per 10 million inhabitants, more HSCTs were registered from unrelated 16 433 donors than related 15 493 donors. Grafts were collected from peripheral blood (66%), bone marrow (24%; mainly non-malignant disorders) and cord blood (10%). Compared with 2006, an increase of 46% total (57% allogeneic and 38% autologous) was observed. Growth was due to an increase in reporting teams (18%) and median transplant activity/team (from 38 to 48 HSCTs/team). An increase of 167% was noted in mismatched/haploidentical family HSCT. A Strengths, Weaknesses, Opportunities, Threats (SWOT) analysis revealed the global perspective of WBMT to be its major strength and identified potential to be the key professional body for patients and authorities. The limited data collection remains its major weakness and threat. In conclusion, global HSCT grows over the years without plateauing (allogeneic>autologous) and at different rates in the four World Health Organization regions. Major increases were observed in allogeneic, haploidentical HSCT and, to a lesser extent, in cord blood transplantation.

  17. Protein interaction module-assisted function X (PIMAX) approach to producing challenging proteins including hyperphosphorylated tau and active CDK5/p25 kinase complex.

    PubMed

    Sui, Dexin; Xu, Xinjing; Ye, Xuemei; Liu, Mengyu; Mianecki, Maxwell; Rattanasinchai, Chotirat; Buehl, Christopher; Deng, Xiexiong; Kuo, Min-Hao

    2015-01-01

    Many biomedically critical proteins are underrepresented in proteomics and biochemical studies because of the difficulty of their production in Escherichia coli. These proteins might possess posttranslational modifications vital to their functions, tend to misfold and be partitioned into bacterial inclusion bodies, or act only in a stoichiometric dimeric complex. Successful production of these proteins requires efficient interaction between these proteins and a specific "facilitator," such as a protein-modifying enzyme, a molecular chaperone, or a natural physical partner within the dimeric complex. Here we report the design and application of a protein interaction module-assisted function X (PIMAX) system that effectively overcomes these hurdles. By fusing two proteins of interest to a pair of well-studied protein-protein interaction modules, we were able to potentiate the association of these two proteins, resulting in successful production of an enzymatically active cyclin-dependent kinase complex and hyperphosphorylated tau protein, which is intimately linked to Alzheimer disease. Furthermore, using tau isoforms quantitatively phosphorylated by GSK-3β and CDK5 kinases via PIMAX, we demonstrated the hyperphosphorylation-stimulated tau oligomerization in vitro, paving the way for new Alzheimer disease drug discoveries. Vectors for PIMAX can be easily modified to meet the needs of different applications. This approach thus provides a convenient and modular suite with broad implications for proteomics and biomedical research.

  18. REVIEW: Accuracies of Global Land Cover Maps Checked against Fluxnet Sites

    NASA Astrophysics Data System (ADS)

    Gong, Peng

    2008-01-01

    Global land cover data products are key sources of information in understanding the complex interactions between human activities and global change. They play a critical role in improving performances of ecosystem, hydrological and atmospheric models. Three freely available global land cover products developed in the United States are popularly used by the scientific community. These include two global maps developed separately by the United States Geological Survey (USGS) and the University of Maryland (UMD) with NOAA Advanced Very High Resolution Radiometer (AVHRR) data, and one developed by Boston University with the EOS Moderate Resolution Imaging Spectroradiometer (MODIS) data. They are compared with known land cover types at 250 available Fluxnet sites around the world. The overall accuracies are 37%, 36% and 42%, respectively for the USGS, UMD and Boston global land cover maps. Some future global land cover mapping strategies are suggested.

  19. Challenges and solutions in the bioanalysis of BMS-986094 and its metabolites including a highly polar, active nucleoside triphosphate in plasma and tissues using LC-MS/MS.

    PubMed

    Liu, Ang; Lute, John; Gu, Huidong; Wang, Bonnie; Trouba, Kevin J; Arnold, Mark E; Aubry, Anne-Françoise; Wang, Jian

    2015-09-01

    BMS-986094, a nucleotide polymerase inhibitor of the hepatitis C virus, was withdrawn from clinical trials because of a serious safety issue. To investigate a potential association between drug/metabolite exposure and toxicity in evaluations conducted after the termination of the BMS-986094 development program, it was essential to determine the levels of BMS-986094 and its major metabolites INX-08032, INX-08144 and INX-09054 in circulation and the active nucleoside triphosphate INX-09114 in target and non-target tissues. However, there were many challenges in the bioanalysis of these compounds. The chromatography challenge for the extremely polar nucleoside triphosphate was solved by applying mixed-mode chromatography which combined anion exchange and reversed-phase interactions. The LC conditions provided adequate retention and good peak shape of the analyte and showed good robustness. A strategy using simultaneous extraction but separate LC analysis of the prodrug BMS-986094 and its major circulating metabolites was used to overcome a carryover issue of the hydrophobic prodrug while still achieving good chromatography of the polar metabolites. In addition, the nucleotide analytes were not stable in the presence of endogenous enzymes. Low pH and low temperature were required for blood collection and plasma sample processing. However, the use of phosphatase inhibitor and immediate homogenization and extraction were critical for the quantitative analysis of the active triphosphate, INX-09114, in tissue samples. To alleviate the bioanalytical complexity caused by multiple analytes, different matrices, and various species, a fit-for-purpose approach to assay validation was implemented based on the needs of drug safety assessment in non-clinical (GLP or non-GLP) studies. The assay for INX-08032 was fully validated in plasma of toxicology species. The lower limit of quantification was 1.00ng/mL and the linear curve range was 1.00-500.00ng/mL using a weighted (1/x(2

  20. Challenges and solutions in the bioanalysis of BMS-986094 and its metabolites including a highly polar, active nucleoside triphosphate in plasma and tissues using LC-MS/MS.

    PubMed

    Liu, Ang; Lute, John; Gu, Huidong; Wang, Bonnie; Trouba, Kevin J; Arnold, Mark E; Aubry, Anne-Françoise; Wang, Jian

    2015-09-01

    BMS-986094, a nucleotide polymerase inhibitor of the hepatitis C virus, was withdrawn from clinical trials because of a serious safety issue. To investigate a potential association between drug/metabolite exposure and toxicity in evaluations conducted after the termination of the BMS-986094 development program, it was essential to determine the levels of BMS-986094 and its major metabolites INX-08032, INX-08144 and INX-09054 in circulation and the active nucleoside triphosphate INX-09114 in target and non-target tissues. However, there were many challenges in the bioanalysis of these compounds. The chromatography challenge for the extremely polar nucleoside triphosphate was solved by applying mixed-mode chromatography which combined anion exchange and reversed-phase interactions. The LC conditions provided adequate retention and good peak shape of the analyte and showed good robustness. A strategy using simultaneous extraction but separate LC analysis of the prodrug BMS-986094 and its major circulating metabolites was used to overcome a carryover issue of the hydrophobic prodrug while still achieving good chromatography of the polar metabolites. In addition, the nucleotide analytes were not stable in the presence of endogenous enzymes. Low pH and low temperature were required for blood collection and plasma sample processing. However, the use of phosphatase inhibitor and immediate homogenization and extraction were critical for the quantitative analysis of the active triphosphate, INX-09114, in tissue samples. To alleviate the bioanalytical complexity caused by multiple analytes, different matrices, and various species, a fit-for-purpose approach to assay validation was implemented based on the needs of drug safety assessment in non-clinical (GLP or non-GLP) studies. The assay for INX-08032 was fully validated in plasma of toxicology species. The lower limit of quantification was 1.00ng/mL and the linear curve range was 1.00-500.00ng/mL using a weighted (1/x(2

  1. Aryl hydrocarbon receptor SNP -130 C/T associates with dioxins susceptibility through regulating its receptor activity and downstream effectors including interleukin 24.

    PubMed

    Liu, Ge; Asanoma, Kazuo; Takao, Tomoka; Tsukimori, Kiyomi; Uchi, Hiroshi; Furue, Masutaka; Kato, Kiyoko; Wake, Norio

    2015-01-22

    Dioxins are persistent environmental pollutants that cause multiple adverse health effects in humans, mainly through binding to the ligand-activated transcription factor, aryl hydrocarbon receptor (AhR). Genetic variation in AhR may modulate the susceptibility to dioxins. In this study, we aimed to evaluate the effects of the single nucleotide polymorphism (SNP) -130 C/T in the AhR promoter on dioxin-inducible gene transcription, and to investigate interleukin-24 (IL-24) and interleukin-1β (IL-1β) as proxies for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure. Using primary human chorionic stromal cells, we found that cells with the TT genotype showed higher AhR mRNA and protein levels than did those of the CC genotype. Microarray was carried out to analyze the gene expression profiles of cells (CC and TT genotype) after exposing the cells to TCDD. Several genes associated with human disorders were more highly up-regulated in cells of the TT genotype. Higher up-regulation of IL-24 and IL-1β mRNA in cells with the TT genotype was observed. Furthermore, blood samples from 64 Yusho patients who were accidentally exposed to high concentrations of dioxins were analyzed for the genotype, dioxins concentrations and serum levels of IL-24 and IL-1β. We observed higher serum IL-24 levels and lower serum IL-1β levels in Yusho patients with the TT genotype than in those with the CC genotype. AhR SNP -130 C/T affects serum IL-24 and IL-1β levels, independently of serum dioxins concentrations in Yusho patients. Our observations demonstrate that SNP -130 C/T modulates AhR expression and expression levels of IL-24 and IL-1β, and suggest an association of AhR SNP -130 C/T with the susceptibility to dioxins.

  2. Benthic food web structure in the Comau fjord, Chile (∼42°S): Preliminary assessment including a site with chemosynthetic activity

    NASA Astrophysics Data System (ADS)

    Zapata-Hernández, Germán; Sellanes, Javier; Mayr, Christoph; Muñoz, Práxedes

    2014-12-01

    Using C and N stable isotopes we analyzed different trophic aspects of the benthic fauna at two sites in the Comau fjord: one with presence of venting of chemically reducing fluids and extensive patches of bacterial mats (XH: X-Huinay), and one control site (PG: Punta Gruesa) with a typical fjord benthic habitat. Due to the widespread presence of such microbial patches in the fjord and their recognized trophic role in reducing environments, we hypothesize that these microbial communities could be contributing to the assimilated food of consumers and transferring carbon into high trophic levels in the food web. Food sources in the area included macroalgae with a wide range of δ13C values (-34.7 to -11.9‰), particulate organic matter (POM, δ13C = -20.1‰), terrestrial organic matter (TOM, δ13C = -32.3‰ to -27.9‰) and chemosynthetic filamentous bacteria (δ13C = ∼-33‰). At both sites, fauna depicted typical values indicating photosynthetic production as a main food source (>-20‰). However, at XH selected taxa reported lower δ13C values (e.g. -26.5‰ in Nacella deaurata), suggesting a partial use of chemosynthetic production. Furthermore, enhanced variability at this site in δ13C values of the polyplacophoran Chiton magnificus, the limpet Fissurella picta and the tanaid Zeuxoides sp. may also be responding to the use of a wider scope of primary food sources. Trophic position estimates suggest three trophic levels of consumers at both sites. However, low δ15N values in some grazer and suspension-feeder species suggest that these taxa could be using other sources still to be identified (e.g. bacterial films, microalgae and organic particles of small size-fractions). Furthermore, between-site comparisons of isotopic niche width measurements in some trophic guilds indicate that grazers from XH have more heterogenic trophic niches than at PG (measured as mean distance to centroid and standard deviation of nearest neighbor distance). This last could be

  3. Comparing bottom-up and top-down approaches at the landscape scale, including agricultural activities and water systems, at the Roskilde Fjord, Denmark

    NASA Astrophysics Data System (ADS)

    Lequy, Emeline; Ibrom, Andreas; Ambus, Per; Massad, Raia-Silvia; Markager, Stiig; Asmala, Eero; Garnier, Josette; Gabrielle, Benoit; Loubet, Benjamin

    2015-04-01

    The greenhouse gas nitrous oxide (N2O) mainly originates in direct emissions from agricultural soils due to microbial reactions stimulated by the use of nitrogen fertilisers. Indirect N2O emissions from water systems due to nitrogen leaching and deposition from crop fields range between 26 and 37% of direct agricultural emissions, indicating their potential importance and uncertainty (Reay et al. 2012). The study presented here couples a top-down approach with eddy covariance (EC) and a bottom-up approach using different models and measurements. A QCL sensor at 96-m height on a tall tower measures the emissions of N2O from 1100 ha of crop fields and from the south part of the Roskilde fjord, in a 5-km radius area around the tall tower at Roskilde, Denmark. The bottom-up approach includes ecosystem modelling with CERES-EGC for the crops and PaSIM for the grasslands, and the N2O fluxes from the Roskilde fjord are derived from N2O sea water concentration measurements. EC measurements are now available from July to December 2014, and indicate a magnitude of the emissions from the crop fields around 0.2 mg N2O-N m-2 day-1 (range -9 to 5) which is consistent with the CERES-EGC simulations and calculations using IPCC emission factors. N2O fluxes from the Roskilde fjord in May and July indicated quite constant N2O concentrations around 0.1 µg N L-1 despite variations of nitrate and ammonium in the fjord. The calculated fluxes from these concentrations and the tall tower measurements consistently ranged between -7 and 6 mg N2O-N m-2 day-1. The study site also contains a waste water treatment plant, whose direct emissions will be measured in early 2015 using a dynamic plume tracer dispersion method (Mønster et al. 2014). A refined source attribution methodology together with more measurements and simulations of the N2O fluxes from the different land uses in this study site will provide a clearer view of the dynamics and budgets of N2O at the regional scale. The

  4. VEGETATIVE COVERS FOR WASTE CONTAINMENT

    EPA Science Inventory

    Disposal of municipal ahd hazardous waste in the United States is primarily accomplished by containment in lined and capped landfills. Evapotranspiration cover systems offer an alternative to conventional landfill cap systems. These covers work on completely different principles ...

  5. Pump apparatus including deconsolidator

    SciTech Connect

    Sonwane, Chandrashekhar; Saunders, Timothy; Fitzsimmons, Mark Andrew

    2014-10-07

    A pump apparatus includes a particulate pump that defines a passage that extends from an inlet to an outlet. A duct is in flow communication with the outlet. The duct includes a deconsolidator configured to fragment particle agglomerates received from the passage.

  6. Cover crops and N credits

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cover crops often provide many short- and long-term benefits to cropping systems. Legume cover crops can significantly reduce the N fertilizer requirement of non-legume cash crops that follow. The objectives of this presentation were to: I) educate stakeholders about the potential benefits of cover ...

  7. Crown cover chart for oak savannas. Forest Service technical brief

    SciTech Connect

    Law, J.R.; Johnson, P.S.; Houf, G.

    1994-07-01

    Although oak savannas have been defined in many ways, they are characterized by scattered trees, largely comprised of oaks, and a sparse ground layer rich in grasses and forbs. The crown cover chart can be used to estimate the crown cover of trees as a percent of total area. Potential applications of the chart include monitoring changes in savanna crown cover, determining needed reductions in crown cover, and defining the savanna state. in restoring savannas that have grown into closed canopy stands, one can use the chart to estimate initial crown cover before restoration work is begun and again after crown cover has been reduced.

  8. Summary of decontamination cover manufacturing experience

    SciTech Connect

    Ulrich, G.B.; Berry, H.W.

    1995-02-01

    Decontamination cover forming cracks and vent cup assembly leaks through the decontamination covers were early manufacturing problems. The decontamination cover total manufacturing process yield was as low as 55%. Applicable tooling and procedures were examined. All manufacturing steps from foil fabrication to final assembly leak testing were considered as possible causes or contributing factors to these problems. The following principal changes were made to correct these problems: (1) the foil annealing temperature was reduced from 1375{degrees} to 1250{degrees}C, (2) the decontamination cover fabrication procedure (including visual inspection for surface imperfections and elimination of superfluous operations) was improved, (3) the postforming dye penetrant inspection procedure was revised for increased sensitivity, (4) a postforming (prewelding) 1250{degrees}C/1 h vacuum stress-relief operation was added, (5) a poststress relief (prewelding) decontamination cover piece-part leak test was implemented, (6) the hold-down fixture used during the decontamination cover-to-cup weld was modified, and concomitantly, and (7) the foil fabrication process was changed from the extruding and rolling of 63-mm-diam vacuum arc-remelted ingots (extrusion process) to the rolling of 19-mm-square arc-melted drop castings (drop cast process). Since these changes were incorporated, the decontamination cover total manufacturing process yield has been 91 %. Most importantly, more than 99% of the decontamination covers welded onto vent cup assemblies were acceptable. The drastic yield improvement is attributed primarily to the change in the foil annealing temperature from 1375{degrees} to 1250{degrees}C and secondarily to the improvements in the decontamination cover fabrication procedure.

  9. Characteristics of seasonal vegetation cover in the conterminous USA

    USGS Publications Warehouse

    Gallo, Kevin P.; Reed, Bradley C.; Owen, editors, Timothy W.; Adegoke, Jimmy O.

    2005-01-01

    A data set of the fractional green vegetation cover (FGREEN) for the Conterminous USA was evaluated for regional and seasonal variation. The value of FGREEN was derived monthly for the three most dominant land cover classes per 20 km by 20 km grid cell within the study area. At this grid cell resolution (comprised of 400 1-km pixels), 97 percent of the grid cells included three or fewer land cover classes. FGREEN was found to vary regionally due to local land cover and climate variations. FGREEN was found significantly different between one or more of the land cover classes, for one or more months, in 58 percent of the grid cells included in the study. Monthly FGREEN values for the land cover classes vary sufficiently between the land cover classes to warrant monthly FGREEN data for each of the one to three most dominant land cover classes per grid cell.

  10. Are You Covered?

    ERIC Educational Resources Information Center

    Liddell, Jamise

    1999-01-01

    Campus event planners need to know all they can about the risky business of special-events insurance, including coverage types and options (institutional vs. outside-user, workers' compensation, cancellation/abandonment, nonappearance, weather, death/disgrace, boutique/niche insurance), basic insurance issues, alcohol issues, estimating needs, and…

  11. Astrobiology of Antarctic ice Covered Lakes

    NASA Astrophysics Data System (ADS)

    Doran, P. T.; Fritsen, C. H.

    2005-12-01

    Antarctica contains a number of permanently ice-covered lakes which have often been used as analogs of purported lakes on Mars in the past. Antarctic subglacial lakes, such as Lake Vostok, have also been viewed as excellent analogs for an ice covered ocean on the Jovian moon Europa, and to a lesser extend on Mars. Lakes in the McMurdo Dry Valleys of East Antarctica have ice covers that range from 3 to 20 meters thick. Water salinities range from fresh to hypersaline. The thinner ice-covered lakes have a well-documented ecology that relies on the limited available nutrients and the small amount of light energy that penetrates the ice covers. The thickest ice-covered lake (Lake Vida in Victoria Valley) has a brine beneath 20 m of ice that is 7 times sea water and maintains a temperature below -10 degrees Celsius. This lake is vastly different from the thinner ice-covered lakes in that there is no communication with the atmosphere. The permanent ice cover is so thick, that summer melt waters can not access the sub-ice brine and so the ice grows from the top up, as well as from the bottom down. Brine trapped beneath the ice is believed to be ancient, stranded thousands of years ago when the ice grew thick enough to isolate it from the surface. We view Lake Vida as an excellent analog for the last aquatic ecosystem to have existed on Mars under a planetary cooling. If, as evidence is now increasingly supporting, standing bodies of water existed on Mars in the past, their fate under a cooling would be to go through a stage of permanent ice cover establishment, followed by a thickening of that ice cover until the final stage just prior to a cold extinction would be a Lake Vida-like lake. If dust storms or mass movements covered these ancient lakes, remnants may well be in existence in the subsurface today. A NASA Astrobiology Science and Technology for Exploring Planets (ASTEP) project will drill the Lake Vida ice cover and access the brine and sediments beneath in

  12. Regional land cover characterization using Landsat thematic mapper data and ancillary data sources

    USGS Publications Warehouse

    Vogelmann, J.E.; Sohl, T.L.; Campbell, P.V.; Shaw, D.M.; ,

    1998-01-01

    As part of the activities of the Multi-Resolution Land Characteristics (MRLC) Interagency Consortium, an intermediate-scale land cover data set is being generated for the conterminous United States. This effort is being conducted on a region-by-region basis using U.S. Standard Federal Regions. To date, land cover data sets have been generated for Federal Regions 3 (Pennsylvania, West Virginia, Virginia, Maryland, and Delaware) and 2 (New York and New Jersey). Classification work is currently under way in Federal Region 4 (the southeastern United States), and land cover mapping activities have been started in Federal Regions 5 (the Great Lakes region) and 1 (New England). it is anticipated that a land cover data set for the conterminous United States will be completed by the end of 1999. A standard land cover classification legend is used, which is analogous to and compatible with other classification schemes. The primary MRLC regional classification scheme contains 23 land cover classes. The primary source of data for the project is the Landsat thematic mapper (TM) sensor. For each region, TM scenes representing both leaf-on and leaf-off conditions are acquired, preprocessed, and georeferenced to MRLC specifications. Mosaicked data are clustered using unsupervised classification, and individual clusters are labeled using aerial photographs. Individual clusters that represent more than one land cover unit are split using spatial modeling with multiple ancillary spatial data layers (most notably, digital elevation model, population, land use and land cover, and wetlands information). This approach yields regional land cover information suitable for a wide array of applications, including landscape metric analyses, land management, land cover change studies, and nutrient and pesticide runoff modeling.

  13. Listening to Include

    ERIC Educational Resources Information Center

    Veck, Wayne

    2009-01-01

    This paper attempts to make important connections between listening and inclusive education and the refusal to listen and exclusion. Two lines of argument are advanced. First, if educators and learners are to include each other within their educational institutions as unique individuals, then they will need to listen attentively to each other.…

  14. Validation of Global Land Cover Products using an Independent Global Reference Validation Database

    NASA Astrophysics Data System (ADS)

    Sulla-Menashe, D. J.; Olofsson, P.; Stehman, S. V.; Woodcock, C. E.; Herold, M.; Newell, J.; Sibley, A. M.; Friedl, M. A.

    2010-12-01

    As production of global land cover products from remote sensing becomes more routine, new data sets and methods in support of global land cover validation are urgently needed. In this paper we describe efforts to (1) compile a new global reference land cover validation database, and (2) develop standardized accuracy assessment procedures to validate regional and global land cover products. To this end, we developed a global stratification of land areas by intersecting the Koppen climate classification with gridded population density maps. The resulting stratification includes 21 strata and incorporated the influence of human activity on land cover. This stratification was used to select 500 validation sites based on a stratified random sample design. Because the base stratification is independent of land cover, this design provides an unbiased basis for sampling different land cover maps and can be augmented with reference information or additional sites in specific strata or regions of interest. The response design provides reference classifications at high spatial resolution for 5km x 5km blocks centered on each validation sample point. Interpretation and classification of these blocks is based on segmentation and classification of very high-resolution satellite imagery using the UN FAO Land Cover Classification System. To evaluate this design, we propose a methodology for overlaying the high-resolution reference site classifications on coarse-resolution land cover products such as the MODIS Land Cover Type Product (MCD12Q1). Initial accuracy results are presented in the form of error matrices and measures of agreement between class area proportions. Initial results suggest that high-resolution reference imagery is useful for understanding sources of coarse-resolution mapping errors, especially among mixture classes. As part of this analysis we examine issues of semantic similarity among classes, and explore how this issue affects estimates of classification

  15. Mine Waste Technology Program Electrochemical Tailings Cover

    EPA Science Inventory

    This report summarizes the results of Mine Waste Technology Program (MWTP) Activity III, Project 40, Electrochemical Tailings Cover, funded by the U.S. Environmental Protection Agency (EPA) and jointly administered by EPA and the U.S. Department of Energy (DOE). MSE Technology A...

  16. CoverCAM - a land cover composition change assessment method

    NASA Astrophysics Data System (ADS)

    Ali, A.; Bie, C. D.; Skidmore, A. K.

    2013-12-01

    The cover-composition on a specific piece of land can change over time due to natural and anthropogenic factors. Accurate detection of where and when changes occur requires a method that uses remotely sensed imagery that represents a continuous and consistent record on the state of the green land-cover. Such data are offered through hyper-temporal NDVI-imagery. Until recently, NDVI-images were mainly used for anomaly mapping to monitor the influence of weather on vegetation; the monitoring basically assume that, over time, the land cover composition of a studied area remains static. This study presents a novel cover change assessment method, labelled ';CoverCAM' that extracts from hyper-temporal NDVI-imagery the probabilities that the original land cover composition did change. CoverCAM, unlike all existing change-detection methods, makes adjustments based on seasonal NDVI-anomalies experienced at landscape level. We tested the method by processing SPOT-VGT NDVI-imagery (10-day Maximum Value Composites; 1km pixels) for Andalucía, Spain. CoverCAM requires specification that two time periods are specified: a reference period (we used 2000-04), and a change detection period (we used 2005-10). All images of the reference period were classified using the ISODATA algorithm and by evaluating divergence statistics. The generated map depicts strata (group of polygons), characterized by temporal NDVI and standard deviation (SD) profiles. For the change assessment period, first, mean NDVI-values were calculated by decade and polygon (NDVId,p), and then for each pixel of the polygon its pixel change values specified through the remaining difference between the pixel NDVI and [NDVId,p × the SD value of the stratum for that decade]. The above process was repeated to produce decadal land cover change probability maps, each with its own undefined scale. The decadal change maps were then aggregated to annual change probability maps. This validation was only carried out for

  17. Regulatory guidance on soil cover systems

    SciTech Connect

    Kane, J.D.

    1991-12-31

    The US Nuclear Regulatory Commission (NRC) in September 1991, completed revisions to 14 sections of the Standard Review Plan (SRP) for the Review of a License Application for a Low-Level Radioactive Waste Disposal Facility. The major purposes of the SRP are to ensure the quality and uniformity of the NRC staff`s safety reviews, and to present a well-defined base from which to evaluate the acceptability of information and data provided in the Safety Analysis Report (SAR) portion of the license application. SRP 3.2, entitled, Design Considerations for Normal and Abnormal/Accident Conditions, was one of the sections that was revised by the NRC staff. This revision was completed to provide additional regulatory guidance on the important considerations that need to be addressed for the proper design and construction of soil cover systems that are to be placed over the LLW. The cover system over the waste is acknowledged to be one of the most important engineered barriers for the long-term stable performance of the disposal facility. The guidance in revised SRP 3.2 summarizes the previous efforts and recommendations of the US Army Corps of Engineers (COE), and a peer review panel on the placement of soil cover systems. NRC published these efforts in NUREG/CR-5432. The discussions in this paper highlight selected recommendations on soil cover issues that the NRC staff considers important for ensuring the safe, long-term performance of the soil cover systems. The development phases to be discussed include: (1) cover design; (2) cover material selection; (3) laboratory and field testing; (4) field placement control and acceptance; and (5) penetrations through the constructed covers.

  18. Land cover mapping of North and Central America—Global Land Cover 2000

    USGS Publications Warehouse

    Latifovic, Rasim; Zhu, Zhi-Liang

    2004-01-01

    The Land Cover Map of North and Central America for the year 2000 (GLC 2000-NCA), prepared by NRCan/CCRS and USGS/EROS Data Centre (EDC) as a regional component of the Global Land Cover 2000 project, is the subject of this paper. A new mapping approach for transforming satellite observations acquired by the SPOT4/VGTETATION (VGT) sensor into land cover information is outlined. The procedure includes: (1) conversion of daily data into 10-day composite; (2) post-seasonal correction and refinement of apparent surface reflectance in 10-day composite images; and (3) extraction of land cover information from the composite images. The pre-processing and mosaicking techniques developed and used in this study proved to be very effective in removing cloud contamination, BRDF effects, and noise in Short Wave Infra-Red (SWIR). The GLC 2000-NCA land cover map is provided as a regional product with 28 land cover classes based on modified Federal Geographic Data Committee/Vegetation Classification Standard (FGDC NVCS) classification system, and as part of a global product with 22 land cover classes based on Land Cover Classification System (LCCS) of the Food and Agriculture Organisation. The map was compared on both areal and per-pixel bases over North and Central America to the International Geosphere–Biosphere Programme (IGBP) global land cover classification, the University of Maryland global land cover classification (UMd) and the Moderate Resolution Imaging Spectroradiometer (MODIS) Global land cover classification produced by Boston University (BU). There was good agreement (79%) on the spatial distribution and areal extent of forest between GLC 2000-NCA and the other maps, however, GLC 2000-NCA provides additional information on the spatial distribution of forest types. The GLC 2000-NCA map was produced at the continental level incorporating specific needs of the region.

  19. Light, Including Ultraviolet

    PubMed Central

    Maverakis, Emanual; Miyamura, Yoshinori; Bowen, Michael P.; Correa, Genevieve; Ono, Yoko; Goodarzi, Heidi

    2009-01-01

    Ultraviolet (UV) light is intricately linked to the functional status of the cutaneous immune system. In susceptible individuals, UV radiation can ignite pathogenic inflammatory pathways leading to allergy or autoimmunity. In others, this same UV radiation can be used as a phototherapy to suppress pathogenic cutaneous immune responses. These vastly different properties are a direct result of UV light’s ability to ionize molecules in the skin and thereby chemically alter them. Sometimes these UV-induced chemical reactions are essential, the formation of pre-vitamin D3 from 7-dehydrocholesterol, for example. In other instances they can be potentially detrimental. UV radiation can ionize a cell’s DNA causing adjacent pyrimidine bases to chemically bond to each other. To prevent malignant transformation, a cell may respond to this UV-induced DNA damage by undergoing apoptosis. Although this pathway prevents skin cancer it also has the potential of inducing or exacerbating autoreactive immune responses by exposing the cell’s nuclear antigens. Ultaviolet-induced chemical reactions can activate the immune system by a variety of other mechanisms as well. In response to UV irradiation keratinocytes secrete cytokines and chemokines, which activate and recruit leukocytes to the skin. In some individuals UV-induced chemical reactions can synthesize novel antigens resulting in a photoallergy. Alternatively, photosensitizing molecules can damage cells by initiating sunburn-like phototoxic reactions. Herein we review all types of UV-induced skin reactions, especially those involving the immune system. PMID:20018479

  20. FLOOR COVERING--PART 1, RESILIENT COVERINGS. WORKBOOK.

    ERIC Educational Resources Information Center

    DUTTER, RALPH; AND OTHERS

    THE INFORMATION IN THIS STUDY GUIDE WAS DEVELOPED FOR USE IN RELATED TECHNICAL INSTRUCTION IN APPRENTICE TRAINING FOR THE FLOOR COVERING TRADE. THE MATERIAL WAS WRITTEN BY TRADE INSTRUCTORS AND JOURNEYMEN UNDER THE DIRECTION AND COORDINATION OF THE STATE EDUCATIONAL ADVISORY COMMITTEE FOR THE FLOOR COVERING TRADE AND OTHERS. THE UNITS ARE (1)…

  1. 39 CFR 960.3 - Proceedings covered.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Service UNITED STATES POSTAL SERVICE PROCEDURES RULES RELATIVE TO IMPLEMENTATION OF THE EQUAL ACCESS TO... initiating the proceeding or designating the matter for hearing. The failure to designate a proceeding as an... proceedings on the application. (c) If a proceeding includes both matters covered by the Act and...

  2. 22 CFR 171.41 - Covered employees.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... PUBLIC Ethics in Government Act Provisions § 171.41 Covered employees. (a) Officers and employees (including special Government employees as defined in 18 U.S.C. 202) whose positions are classified at grades... Director of the Office of Government Ethics to be of equal classification to GS-16; (c) Employees in...

  3. 22 CFR 171.41 - Covered employees.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... PUBLIC Ethics in Government Act Provisions § 171.41 Covered employees. (a) Officers and employees (including special Government employees as defined in 18 U.S.C. 202) whose positions are classified at grades... Director of the Office of Government Ethics to be of equal classification to GS-16; (c) Employees in...

  4. Northern Hemisphere snow cover and atmospheric blocking variability

    NASA Astrophysics Data System (ADS)

    GarcíA-Herrera, Ricardo; Barriopedro, David

    2006-11-01

    The interseasonal relationship between Northern Hemisphere (NH) snow cover and regional blocking patterns is explored for a 31-year data set. It is found that snow cover exerts an important influence on regional atmospheric blocking, which, in turn, modulates snow cover extent at subcontinental scales. Observational results provide strong evidence of two primary linkages in the seasonal snow cover-blocking relationship that support an interannual persistence cycle: The first one links winter blocking over the Atlantic and the subsequent spring (summer) Eurasian (North American) snow cover anomalies; the second one implies that spring (summer) Eurasian (North American) snow cover precedes an anomalous winter Atlantic blocking activity. We describe the temporal stages of the snow cover-blocking relationship in the framework of a six-step conceptual model. According to that, an enhanced Atlantic blocking activity in winter favors a later spring snow disappearance through an enhanced cold advection toward western Eurasia. The resulting snow cover anomalies partially force an opposite-sign blocking response over west and central Pacific which is sustained through spring and early summer, presumably because of the persistence of snow cover anomalies. This anomalous pattern seems to play a role in the propagation of snow cover anomalies from Eurasia in spring to the Hudson's Bay region of North America in summer. The excessive snow cover over this region induces an asymmetrical temperature distribution, which, in turn, favors blocking activity over Europe and the West Pacific. The connection between autumn and winter climates is not clear but it could be related with the ability of autumn high ATL blocking activity to determine an early snow cover appearance in October over western Eurasia. This linkage completes a snow cover-blocking cycle of interactions which identifies snow cover as a candidate for the recently observed blocking trends and a contributor to the

  5. Cellular uptake, antioxidant and antiproliferative activity of entrapped α-tocopherol and γ-tocotrienol in poly (lactic-co-glycolic) acid (PLGA) and chitosan covered PLGA nanoparticles (PLGA-Chi).

    PubMed

    Alqahtani, Saeed; Simon, Lacey; Astete, Carlos E; Alayoubi, Alaadin; Sylvester, Paul W; Nazzal, Sami; Shen, Yixiao; Xu, Zhimin; Kaddoumi, Amal; Sabliov, Cristina M

    2015-05-01

    The aim of this study was to formulate and characterize α-tocopherol (α-T) and tocotrienol-rich fraction (TRF) entrapped in poly (lactide-co-glycolide) (PLGA) and chitosan covered PLGA (PLGA-Chi) based nanoparticles. The resultant nanoparticles were characterized and the effect of nanoparticles entrapment on the cellular uptake, antioxidant, and antiproliferative activity of α-T and TRF were tested. In vitro uptake studies in Caco2 cells showed that PLGA and PLGA-Chi nanoparticles displayed a greater enhancement in the cellular uptake of α-T and TRF when compared with the control without causing toxicity to the cells (p<0.0001). Furthermore, the cellular internalization of both PLGA and PLGA-Chi nanoparticles labeled with FITC was investigated by fluorescence microscopy; both types of nanoparticles were able to get internalized into the cells with reasonable amounts. However, PLGA-Chi nanoparticles showed significantly higher (3.5-fold) cellular uptake compared to PLGA nanoparticles. The antioxidant activity studies demonstrated that entrapment of α-T and TRF in PLGA and PLGA-Chi nanoparticles exhibited greater ability in inhibiting cholesterol oxidation at 48 h compared to the control. In vitro antiproliferative studies confirmed marked cytotoxicity of TRF on MCF-7 and MDA-MB-231 cell lines when delivered by PLGA and PLGA-Chi nanoparticles after 48 h incubation compared to control. In summary, PLGA and PLGA-Chi nanoparticles may be considered as an attractive and promising approach to enhance the bioavailability and activity of poorly water soluble compounds such as α-tocopherol and tocotrienols.

  6. The Middeck Active Control Experiment (MACE)

    NASA Technical Reports Server (NTRS)

    Miller, David W.

    1992-01-01

    Viewgraphs on the Middeck Active Control Experiment (MACE) are presented. Topics covered include: program objectives; program features; flight experiment features; current activities; MACE development model lab testing; MACE test article deployed on STS middeck; and development model testing.

  7. Midwest Cover Crops Field Guide

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Producers who want to prevent soil erosion, improve nutrient cycling, sustain their soils, and protect/maintain the environment have been returning to a very old practice: planting cover crops. Cover crops are effective tools for reducing soil erosion and increasing nutrient recycling on farmlands, ...

  8. The dust covering factor in AGN

    NASA Astrophysics Data System (ADS)

    Stalevski, Marko

    2016-08-01

    We undertook a critical investigation of a common estimator of the dust covering factor in active galactic nuclei (AGN). The infrared radiation emitted by the obscuring dusty structure ("the dusty torus") is nothing but a reprocessed fraction of the accretion disk emission, so the ratio of their luminosities (L_torus /L_AGN) should correspond to the fraction of the AGN sky obscured by dust. Using state-of-the-art Monte Carlo radiative transfer code, we calculated a grid of spectral energy distributions (SEDs) emitted by the clumpy two-phase dusty structure. Using this grid of SEDs, we studied the relation between L_torus /L_AGN and the dust covering factor for different parameters of the torus. We found that in case of type 1 AGNs, due to the torus anisotropy, L_torus/L AGN underestimate low covering factors and overestimate high covering factors. In type 2 AGNs covering factors are always underestimated. Our results provide a novel easy-to-use method to account for anisotropy and obtain correct covering factors. Using two samples from the literature, we demonstrated the importance of these effects for inferring the obscured AGN fraction. We found that after the anisotropy is properly accounted for, the dust covering factors show very weak dependence on L_AGN, with values in the range of approx. 0.6 ‑ 0.7. Our results suggest a higher fraction of obscured AGNs at high luminosities than those found by X-ray surveys. We discuss the possible causes of this discrepancy and demonstrate that it is partially due to the presence of a Compton-thick AGN population, which is missed by X-ray surveys, but not by infrared.

  9. AVHRR channel selection for land cover classification

    USGS Publications Warehouse

    Maxwell, S.K.; Hoffer, R.M.; Chapman, P.L.

    2002-01-01

    Mapping land cover of large regions often requires processing of satellite images collected from several time periods at many spectral wavelength channels. However, manipulating and processing large amounts of image data increases the complexity and time, and hence the cost, that it takes to produce a land cover map. Very few studies have evaluated the importance of individual Advanced Very High Resolution Radiometer (AVHRR) channels for discriminating cover types, especially the thermal channels (channels 3, 4 and 5). Studies rarely perform a multi-year analysis to determine the impact of inter-annual variability on the classification results. We evaluated 5 years of AVHRR data using combinations of the original AVHRR spectral channels (1-5) to determine which channels are most important for cover type discrimination, yet stabilize inter-annual variability. Particular attention was placed on the channels in the thermal portion of the spectrum. Fourteen cover types over the entire state of Colorado were evaluated using a supervised classification approach on all two-, three-, four- and five-channel combinations for seven AVHRR biweekly composite datasets covering the entire growing season for each of 5 years. Results show that all three of the major portions of the electromagnetic spectrum represented by the AVHRR sensor are required to discriminate cover types effectively and stabilize inter-annual variability. Of the two-channel combinations, channels 1 (red visible) and 2 (near-infrared) had, by far, the highest average overall accuracy (72.2%), yet the inter-annual classification accuracies were highly variable. Including a thermal channel (channel 4) significantly increased the average overall classification accuracy by 5.5% and stabilized inter-annual variability. Each of the thermal channels gave similar classification accuracies; however, because of the problems in consistently interpreting channel 3 data, either channel 4 or 5 was found to be a more

  10. The value of snow cover

    NASA Astrophysics Data System (ADS)

    Sokratov, S. A.

    2009-04-01

    only and not even the main outcome from snow cover use. The value of snow cover for agriculture, water resources, industry and transportation is so naturally inside the activities that is not often quantified. However, any considerations of adaptation strategies for climate change with changing snow conditions need such quantification.

  11. Blast noise propagation above a snow cover.

    PubMed

    Albert, D G; Hole, L R

    2001-06-01

    A porous medium model of a snow cover, rather than a viscoelastic treatment, has been used to simulate measured, horizontally traveling acoustic waveform propagation above a dry snow cover 11-20 cm thick. The waveforms were produced by explosions of 1-kg charges at propagation distances of 100 to 1400 m. These waveforms, with a peak frequency around 30 Hz, show pulse broadening effects similar to those previously seen for higher-frequency waves over shorter propagation distances. A rigid-ice-frame porous medium ("rigid-porous") impedance model, which includes the effect of the pores within the snow but ignores any induced motion of the ice particles, is shown to produce much better agreement with the measured waveforms compared with a viscoelastic solid treatment of the snow cover. From the acoustic waveform modeling, the predicted average snow cover depth of 18 cm and effective flow resistivities of 16-31 kPa s m(-2) agree with snow pit observations and with previous acoustic measurements over snow. For propagation in the upwind direction, the pulse broadening caused by the snow cover interaction is lessened, but the overall amplitude decay is greater because of refraction of the blast waves. PMID:11425110

  12. In vitro activity of the new fluoroketolide solithromycin (CEM-101) against a large collection of clinical Neisseria gonorrhoeae isolates and international reference strains, including those with high-level antimicrobial resistance: potential treatment option for gonorrhea?

    PubMed

    Golparian, Daniel; Fernandes, Prabhavathi; Ohnishi, Makoto; Jensen, Jörgen S; Unemo, Magnus

    2012-05-01

    Gonorrhea may become untreatable, and new treatment options are essential. We investigated the in vitro activity of the first fluoroketolide, solithromycin. Clinical Neisseria gonorrhoeae isolates and reference strains (n = 246), including the two extensively drug-resistant strains H041 and F89 and additional isolates with clinical cephalosporin resistance and multidrug resistance, were examined. The activity of solithromycin was mainly superior to that of other antimicrobials (n = 10) currently or previously recommended for gonorrhea treatment. Solithromycin might be an effective treatment option for gonorrhea.

  13. Greenland soil bacteria & biogeochemistry: a vegetation cover proxy for climate warming effects

    NASA Astrophysics Data System (ADS)

    Dowdy, K. L.; Sistla, S.; Buckeridge, K. M.; Schimel, J.; Schaeffer, S. M.

    2013-12-01

    Climate warming in the high Arctic is expected to increase plant biomass, deepen thaw, and stimulate decomposition of soil organic matter. However, it remains unclear how warming, plant growth, and microbial processing will interact to drive Arctic carbon and nutrient cycling. For example, greater plant growth should increase carbon storage in the ecosystem; however, increasing plant C inputs and thawing permafrost carbon should stimulate microbial biomass, potentially causing soil respiration to outpace storage. Alternatively, greater plant cover may lower soil temperature through shading, potentially curtailing the predicted increase in microbial activity. To evaluate microbial responses to climate warming in the high Arctic, we characterized the soil bacterial community and related soil biogeochemical properties, including pH, temperature, moisture, bulk density, extractable nutrient pools, extractable organic carbon and nitrogen, and total microbial biomass along a vegetation cover gradient in northwest Greenland. Vegetation cover was classified using the Normalized Difference Vegetation Index (NDVI), and vegetation cover classes were used as a proxy for changes associated with warming. We found that soil moisture increased and soil temperature decreased significantly with vegetation cover; moisture and temperature were higher in organic than in mineral horizons. Extractable nutrients (NO3-, NH4+, PO43-) and extractable organic C and N generally increased with vegetation cover and are higher in organic than in mineral horizons within a given vegetation class, with the exception of NO3-, which was comparable between horizons. Despite increases in available carbon and nutrients, microbial biomass carbon in both horizons ultimately decreased with vegetation cover, as did microbial biomass nitrogen in the mineral horizon. Moreover, the relative proportion of microbial biomass carbon to extractable organic carbon decreased with vegetation cover, indicating that

  14. An assessment of hazards caused by electromagnetic interaction on humans present near short-wave physiotherapeutic devices of various types including hazards for users of electronic active implantable medical devices (AIMD).

    PubMed

    Karpowicz, Jolanta; Gryz, Krzysztof

    2013-01-01

    Leakage of electromagnetic fields (EMF) from short-wave radiofrequency physiotherapeutic diathermies (SWDs) may cause health and safety hazards affecting unintentionally exposed workers (W) or general public (GP) members (assisting patient exposed during treatment or presenting there for other reasons). Increasing use of electronic active implantable medical devices (AIMDs), by patients, attendants, and workers, needs attention because dysfunctions of these devices may be caused by electromagnetic interactions. EMF emitted by 12 SWDs (with capacitive or inductive applicators) were assessed following international guidelines on protection against EMF exposure (International Commission on Nonionizing Radiation Protection for GP and W, new European directive 2013/35/EU for W, European Recommendation for GP, and European Standard EN 50527-1 for AIMD users). Direct EMF hazards for humans near inductive applicators were identified at a distance not exceeding 45 cm for W or 62 cm for GP, but for AIMD users up to 90 cm (twice longer than that for W and 50% longer than that for GP because EMF is pulsed modulated). Near capacitive applicators emitting continuous wave, the corresponding distances were: 120 cm for W or 150 cm for both-GP or AIMD users. This assessment does not cover patients who undergo SWD treatment (but it is usually recommended for AIMD users to be careful with EMF treatment).

  15. An Assessment of Hazards Caused by Electromagnetic Interaction on Humans Present near Short-Wave Physiotherapeutic Devices of Various Types Including Hazards for Users of Electronic Active Implantable Medical Devices (AIMD)

    PubMed Central

    Gryz, Krzysztof

    2013-01-01

    Leakage of electromagnetic fields (EMF) from short-wave radiofrequency physiotherapeutic diathermies (SWDs) may cause health and safety hazards affecting unintentionally exposed workers (W) or general public (GP) members (assisting patient exposed during treatment or presenting there for other reasons). Increasing use of electronic active implantable medical devices (AIMDs), by patients, attendants, and workers, needs attention because dysfunctions of these devices may be caused by electromagnetic interactions. EMF emitted by 12 SWDs (with capacitive or inductive applicators) were assessed following international guidelines on protection against EMF exposure (International Commission on Nonionizing Radiation Protection for GP and W, new European directive 2013/35/EU for W, European Recommendation for GP, and European Standard EN 50527-1 for AIMD users). Direct EMF hazards for humans near inductive applicators were identified at a distance not exceeding 45 cm for W or 62 cm for GP, but for AIMD users up to 90 cm (twice longer than that for W and 50% longer than that for GP because EMF is pulsed modulated). Near capacitive applicators emitting continuous wave, the corresponding distances were: 120 cm for W or 150 cm for both—GP or AIMD users. This assessment does not cover patients who undergo SWD treatment (but it is usually recommended for AIMD users to be careful with EMF treatment). PMID:24089662

  16. Electrically shielded enclosure with magnetically retained removable cover

    DOEpatents

    Rivers, Craig J.; Lee, Roanne A.; Jones, Glenn E.

    1996-01-01

    Disclosed is an electrically shielded enclosure having electrical components therein and a removable electrically shielded cover over an opening in the enclosure with a magnetic securement mechanism provided to removably secure the cover to the enclosure in a manner which will provide easy access, yet also provide an electrical seal between the cover and the enclosure capable of preventing the passage of electrical radiation through the joint between the cover and the enclosure. Magnets are provided on the enclosure peripherally around the opening and facing the cover, and a ferromagnetic surface is provided on the mating surface of the cover facing the magnets, with a continuous electrical seal provided between the magnets and the ferromagnetic surface on the cover to prevent the leakage of electromagnetic radiation therethrough. In one embodiment the electrical seal includes a flexible metal casing or surface, which is attached to the enclosure and positioned between the magnets and the ferromagnetic surface on the cover, and which is sufficiently flexible to be capable of conforming to the ferromagnetic surface to provide an electrical seal between the cover and the enclosure. In another embodiment, the electrical seal includes a metal mesh associated with the enclosure and positioned between the magnets on the enclosure and the ferromagnetic surface on the cover. The metal mesh is also capable of conforming to the surface of the ferromagnetic surface to thereby provide an electrical seal between the cover and the enclosure.

  17. Dielectric covered microstrip patch antennas

    NASA Astrophysics Data System (ADS)

    Sharpe, Lisa M.

    1988-11-01

    Microstrip antennas have many properties that make them suitable for airborne and satellite communications systems. These antennas are low in cost and lightweight. For these reasons, Rome Air Development Center is interested in verifying and augmenting existing design models for these antennas. The theory and results are presented for modeling microstrip antennas that are covered with a sheet of dielectric material. There are several reasons for designing a microstrip antenna covered with a dielectric material. This configuration would allow the modeling of antennas with an integrated radome. A cover layer could possibly be used to support a polarizer; to mount additional antenna elements on top of the cover layer to provide bandwidth enhancements; or to be used as a dual frequency antenna.

  18. 30 CFR 18.29 - Access openings and covers, including unused lead-entrance holes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... lead-entrance holes. 18.29 Section 18.29 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION... unused lead-entrance holes. (a) Access openings in explosion-proof enclosures will be permitted only... Figure 1 in Appendix II.) (c) Holes in enclosures that are provided for lead entrances but which are...

  19. Reconstruction of Intensity From Covered Samples

    SciTech Connect

    Barabash, Rozaliya; Watkins, Thomas R; Meisner, Roberta Ann; Burchell, Timothy D; Rosseel, Thomas M

    2015-01-01

    The safe handling of activated samples requires containment and covering the sample to eliminate any potential for contamination. Subsequent characterization of the surface with x-rays ideally necessitates a thin film. While many films appear visually transparent, they are not necessarily x-ray transparent. Each film material has a unique beam attenuation and sometimes have amorphous peaks that can superimpose with those of the sample. To reconstruct the intensity of the underlying activated sample, the x-ray attenuation and signal due to the film needs to be removed from that of the sample. This requires the calculation of unique deconvolution parameters for the film. The development of a reconstruction procedure for a contained/covered sample is described.

  20. Air plasma treatment of liquid covered tissue: long timescale chemistry

    NASA Astrophysics Data System (ADS)

    Lietz, Amanda M.; Kushner, Mark J.

    2016-10-01

    Atmospheric pressure plasmas have shown great promise for the treatment of wounds and cancerous tumors. In these applications, the sample is usually covered by a thin layer of a biological liquid. The reactive oxygen and nitrogen species (RONS) generated by the plasma activate and are processed by the liquid before the plasma produced activation reaches the tissue. The synergy between the plasma and the liquid, including evaporation and the solvation of ions and neutrals, is critical to understanding the outcome of plasma treatment. The atmospheric pressure plasma sources used in these procedures are typically repetitively pulsed. The processes activated by the plasma sources have multiple timescales—from a few ns during the discharge pulse to many minutes for reactions in the liquid. In this paper we discuss results from a computational investigation of plasma-liquid interactions and liquid phase chemistry using a global model with the goal of addressing this large dynamic range in timescales. In modeling air plasmas produced by a dielectric barrier discharge over liquid covered tissue, 5000 voltage pulses were simulated, followed by 5 min of afterglow. Due to the accumulation of long-lived species such as ozone and N x O y , the gas phase dynamics of the 5000th discharge pulse are different from those of the first pulse, particularly with regards to the negative ions. The consequences of applied voltage, gas flow, pulse repetition frequency, and the presence of organic molecules in the liquid on the gas and liquid reactive species are discussed.

  1. Babes and boobs? Analysis of JAMA cover art

    PubMed Central

    Clark, Jocalyn P

    1999-01-01

    Objective To determine the representation of the sexes in JAMA cover art. Design Review of 50 consecutive issues. Setting JAMA, March 1997-March 1998. Main outcome measures Numbers and nature of covers portraying men and women. Results Of the 50 covers, 34 depicted humans. 15 depicted women, 13 men, and 6 were of mixed or indeterminate sex. 11 pictures of women included a child and five included nudity. One cover showed a man with a child (not as a father) and none depicted nudity. Men were depicted exclusively in authoritative roles. Conclusions Much of the cover art gives strong messages about sexual stereotypes that are inappropriate in modern society. JAMA should consider reviewing its policy for choosing cover art. PMID:10600956

  2. Thermal properties for vegetation cover

    NASA Astrophysics Data System (ADS)

    Aleksyutina, D.; Motenko, R.

    2011-12-01

    Different samples of undisturbed vegetation cover were studied under laboratory conditions. Samples were collected from New Chara city, north of the Chita region. Vegetation cover in this area is represented by moss, lichen and tussock growth. Thermal properties were investigated by the I-st type regular mode method (a-calorimeter), the freezing temperature was studied by cryoscopic methods. The dry density of sampled specimens varies from 0.04 to 0.24 g/cm3, and humidity varies from 250 to 375 percent. The freezing temperature depends on moisture content and varies from -0.2 to 0 degrees centigrade. The vegetation cover had low thermal conductivities which varies from 0.05 to 0.46 W/(m*K) in unfrozen conditions, and from 0.07 to 1.14 W/(m*K) in frozen conditions, according to density and moisture content. Diffusivity of samples varies from 0.073*10-6 to 0.114*10-6 m2/s in thawed conditions, and from 0.174*10-6 to 0.584*10-6 m2/s in frozen conditions. The sod (bottom of vegetation cover) had relatively high thermal properties. Thermal properties of vegetation cover and peat (turf) were compared. The thermal conductivity of peat was much higher than thermal conductivity of vegetation cover. This data may be used for modeling of the thickness of the seasonally thawed layer and ground temperature variation. The knowledge of thermal properties of these samples allows us to view vegetation cover as a separate layer of geological section.

  3. Insurance covered and non-covered complementary and alternative medicine utilisation among adults in Taiwan.

    PubMed

    Shih, Shu-Fang; Lew-Ting, Chih-Yin; Chang, Hsing-Yi; Kuo, Ken N

    2008-10-01

    Complementary and alternative medicine (CAM) has been characterized by prevalence, cost, and patterns of use. Factors determining its utilization have also been analyzed, either generally or for specific diseases, but few studies have considered the determinants of its frequency of use. Taiwan's pluralistic health care system and comprehensive insurance program covering Western medicine and traditional Chinese medicine (TCM) provide an interesting case to explore what forms of CAM people use, why and how often they use them. By using Taiwan's 2001 National Health Interview Survey and linking it with National Health Insurance (NHI) claims data, this study aims to investigate the socio-demographic, economic, behavioral and health determinants of use and frequency of use for both non-covered and covered CAM in Taiwan. The former consists of services practiced by non-regulated or non-NHI contracted practitioners and the latter includes those TCM covered by NHI. Our study showed that the determinants of using non-covered and covered CAM differ with respect to socio-demographic and behavioral factors, and health needs. Our evidence suggests that future CAM research must delineate between use and frequency of use to better understand the underlying factors contributing to initiation and continuity of CAM use.

  4. The level of intracellular glutathione is a key regulator for the induction of stress-activated signal transduction pathways including Jun N-terminal protein kinases and p38 kinase by alkylating agents.

    PubMed Central

    Wilhelm, D; Bender, K; Knebel, A; Angel, P

    1997-01-01

    Monofunctional alkylating agents like methyl methanesulfonate (MMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) are potent inducers of cellular stress leading to chromosomal aberrations, point mutations, and cell killing. We show that these agents induce a specific cellular stress response program which includes the activation of Jun N-terminal kinases/stress-activated protein kinases (JNK/SAPKs), p38 mitogen-activated protein kinase, and the upstream kinase SEK1/MKK4 and which depends on the reaction mechanism of the alkylating agent in question. Similar to another inducer of cellular stress, UV irradiation, damage of nuclear DNA by alkylation is not involved in the MMS-induced response. However, in contrast to UV and other inducers of the JNK/SAPKs and p38 pathways, activation of growth factor and G-protein-coupled receptors does not play a role in the MMS response. We identified the intracellular glutathione (GSH) level as critical for JNK/SAPK activation by MMS: enhancing the GSH level by pretreatment of the cells with GSH or N-acetylcysteine inhibits, whereas depletion of the cellular GSH pool causes hyperinduction of JNK/SAPK activity by MMS. In light of the JNK/SAPK-dependent induction of c-jun and c-fos transcription, and the Jun/Fos-induced transcription of xenobiotic-metabolizing enzymes, these data provide a potential critical role of JNK/SAPK and p38 in the induction of a cellular defense program against cytotoxic xenobiotics such as MMS. PMID:9234735

  5. How Scientists Differentiate Between Land Cover Types

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Before scientists can transform raw satellite image data into land cover maps, they must decide on what categories of land cover they would like to use. Categories are simply the types of landscape that the scientists are trying to map and can vary greatly from map to map. For flood maps, there may be only two categories-dry land and wet land-while a standard global land cover map may have seventeen categories including closed shrub lands, savannas, evergreen needle leaf forest, urban areas, and ice/snow. The only requirement for any land cover category is that it have a distinct spectral signature that a satellite can record. As can be seen through a prism, many different colors (wavelengths) make up the spectra of sunlight. When sunlight strikes objects, certain wavelengths are absorbed and others are reflected or emitted. The unique way in which a given type of land cover reflects and absorbs light is known as its spectral signature. Anyone who has flown over the midwestern United States has seen evidence of this phenomenon. From an airplane window, the ground appears as a patchwork of different colors formed by the fields of crops planted there. The varying pigments of the leaves, the amount of foliage per square foot, the age of the plants, and many other factors create this tapestry. Most imaging satellites are sensitive to specific wavelengths of light, including infrared wavelengths that cannot be seen with the naked eye. Passive satellite remote sensors-such as those flown on Landsat 5, Landsat 7, and Terra-have a number of light detectors (photoreceptors) on board that measure the energy reflected or emitted by the Earth. One light detector records only the blue part of the spectrum coming off the Earth. Another observes all the yellow-green light and still another picks up on all the near-infrared light. The detectors scan the Earth's surface as the satellite travels in a circular orbit very nearly from pole-to-pole. To differentiate between types of

  6. The 1980 land cover for the Puget Sound region

    NASA Technical Reports Server (NTRS)

    Shinn, R. D.; Westerlund, F. V.; Eby, J. R.

    1982-01-01

    Both LANDSAT imagery and the video information communications and retrieval software were used to develop a land cover classifiction of the Puget Sound of Washington. Planning agencies within the region were provided with a highly accurate land cover map registered to the 1980 census tracts which could subsequently be incorporated as one data layer in a multi-layer data base. Many historical activities related to previous land cover mapping studies conducted in the Puget Sound region are summarized. Valuable insight into conducting a project with a large community of users and in establishing user confidence in a multi-purpose land cover map derived from LANDSAT is provided.

  7. The National Land Cover Database

    USGS Publications Warehouse

    Homer, Collin H.; Fry, Joyce A.; Barnes, Christopher A.

    2012-01-01

    The National Land Cover Database (NLCD) serves as the definitive Landsat-based, 30-meter resolution, land cover database for the Nation. NLCD provides spatial reference and descriptive data for characteristics of the land surface such as thematic class (for example, urban, agriculture, and forest), percent impervious surface, and percent tree canopy cover. NLCD supports a wide variety of Federal, State, local, and nongovernmental applications that seek to assess ecosystem status and health, understand the spatial patterns of biodiversity, predict effects of climate change, and develop land management policy. NLCD products are created by the Multi-Resolution Land Characteristics (MRLC) Consortium, a partnership of Federal agencies led by the U.S. Geological Survey. All NLCD data products are available for download at no charge to the public from the MRLC Web site: http://www.mrlc.gov.

  8. 16 CFR 1611.2 - General description of products covered.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS STANDARD FOR THE FLAMMABILITY OF VINYL PLASTIC FILM The Standard § 1611.2 General description of products covered. The material covered is nonrigid, unsupported, vinyl plastic film, including transparent... sections 2 and 4 of the Flammable Fabrics Act of 1953, as amended in 1954, set out at 16 CFR part 1609...

  9. "Proximal Sensing" capabilities for snow cover monitoring

    NASA Astrophysics Data System (ADS)

    Valt, Mauro; Salvatori, Rosamaria; Plini, Paolo; Salzano, Roberto; Giusti, Marco; Montagnoli, Mauro; Sigismondi, Daniele; Cagnati, Anselmo

    2013-04-01

    The seasonal snow cover represents one of the most important land cover class in relation to environmental studies in mountain areas, especially considering its variation during time. Snow cover and its extension play a relevant role for the studies on the atmospheric dynamics and the evolution of climate. It is also important for the analysis and management of water resources and for the management of touristic activities in mountain areas. Recently, webcam images collected at daily or even hourly intervals are being used as tools to observe the snow covered areas; those images, properly processed, can be considered a very important environmental data source. Images captured by digital cameras become a useful tool at local scale providing images even when the cloud coverage makes impossible the observation by satellite sensors. When suitably processed these images can be used for scientific purposes, having a good resolution (at least 800x600x16 million colours) and a very good sampling frequency (hourly images taken through the whole year). Once stored in databases, those images represent therefore an important source of information for the study of recent climatic changes, to evaluate the available water resources and to analyse the daily surface evolution of the snow cover. The Snow-noSnow software has been specifically designed to automatically detect the extension of snow cover collected from webcam images with a very limited human intervention. The software was tested on images collected on Alps (ARPAV webcam network) and on Apennine in a pilot station properly equipped for this project by CNR-IIA. The results obtained through the use of Snow-noSnow are comparable to the one achieved by photo-interpretation and could be considered as better as the ones obtained using the image segmentation routine implemented into image processing commercial softwares. Additionally, Snow-noSnow operates in a semi-automatic way and has a reduced processing time. The analysis

  10. Quantification of global gross forest cover loss.

    PubMed

    Hansen, Matthew C; Stehman, Stephen V; Potapov, Peter V

    2010-05-11

    A globally consistent methodology using satellite imagery was implemented to quantify gross forest cover loss (GFCL) from 2000 to 2005 and to compare GFCL among biomes, continents, and countries. GFCL is defined as the area of forest cover removed because of any disturbance, including both natural and human-induced causes. GFCL was estimated to be 1,011,000 km(2) from 2000 to 2005, representing 3.1% (0.6% per year) of the year 2000 estimated total forest area of 32,688,000 km(2). The boreal biome experienced the largest area of GFCL, followed by the humid tropical, dry tropical, and temperate biomes. GFCL expressed as the proportion of year 2000 forest cover was highest in the boreal biome and lowest in the humid tropics. Among continents, North America had the largest total area and largest proportion of year 2000 GFCL. At national scales, Brazil experienced the largest area of GFCL over the study period, 165,000 km(2), followed by Canada at 160,000 km(2). Of the countries with >1,000,000 km(2) of forest cover, the United States exhibited the greatest proportional GFCL and the Democratic Republic of Congo the least. Our results illustrate a pervasive global GFCL dynamic. However, GFCL represents only one component of net change, and the processes driving GFCL and rates of recovery from GFCL differ regionally. For example, the majority of estimated GFCL for the boreal biome is due to a naturally induced fire dynamic. To fully characterize global forest change dynamics, remote sensing efforts must extend beyond estimating GFCL to identify proximate causes of forest cover loss and to estimate recovery rates from GFCL.

  11. Saving the Kilgore Covered Bridge.

    ERIC Educational Resources Information Center

    Clark, Wilma

    1988-01-01

    Describes an American literature class project to save a covered bridge from collapse. Illustrates how student initiative in contacting government agencies and news media, learning the history of the bridge, and raising public awareness about the project led to a joint county agreement to preserve the historic span. (DHP)

  12. COVERING A CORE BY EXTRUSION

    DOEpatents

    Karnie, A.J.

    1963-07-16

    A method of covering a cylindrical fuel core with a cladding metal ms described. The metal is forced between dies around the core from both ends in two opposing skirts, and as these meet the ends turn outward into an annular recess in the dics. By cutting off the raised portion formed by the recess, oxide impurities are eliminated. (AEC)

  13. ESTIMATING AND PROJECTING IMPERVIOUS COVER

    EPA Science Inventory

    Effective methods to estimate and project impervious cover can help identify areas where a watershed is at risk of changing rapidly from one with relatively pristine streams to one with streams with significant symptoms of degradation. In collaboration with the USEPA, Region 4, ...

  14. Resumes, Applications, and Cover Letters

    ERIC Educational Resources Information Center

    Crosby, Olivia

    2009-01-01

    Good resumes, applications, and cover letters broadcast one's abilities. They tell employers how one's qualifications match a job's responsibilities. If these critical preliminaries are constructed well, one has a better chance of landing interviews--and, eventually, a job. This article provides some guidelines for creating resumes and cover…

  15. Sky cover from MFRSR observations: cumulus clouds

    NASA Astrophysics Data System (ADS)

    Kassianov, E.; Barnard, J.; Berg, L. K.; Flynn, C.; Long, C. N.

    2011-01-01

    The diffuse all-sky surface irradiances measured at two nearby wavelengths in the visible spectral range and their model clear-sky counterparts are two main components of a new method for estimating the fractional sky cover of different cloud types, including cumulus clouds. The performance of this method is illustrated using 1-min resolution data from ground-based Multi-Filter Rotating Shadowband Radiometer (MFRSR). The MFRSR data are collected at the US Department of Energy Atmospheric Radiation Measurement (ARM) Climate Research Facility (ACRF) Southern Great Plains (SGP) site during the summer of 2007 and represent 13 days with cumulus clouds. Good agreement is obtained between estimated values of the fractional sky cover and those provided by a well-established independent method based on broadband observations.

  16. Simulation of regional temperature change effect of land cover change in agroforestry ecotone of Nenjiang River Basin in China

    NASA Astrophysics Data System (ADS)

    Liu, Tingxiang; Zhang, Shuwen; Yu, Lingxue; Bu, Kun; Yang, Jiuchun; Chang, Liping

    2016-02-01

    The Northeast China is one of typical regions experiencing intensive human activities within short time worldwide. Particularly, as the significant changes of agriculture land and forest, typical characteristics of pattern and process of agroforestry ecotone change formed in recent decades. The intensive land use change of agroforestry ecotone has made significant change for regional land cover, which had significant impact on the regional climate system elements and the interactions among them. This paper took agroforestry ecotone of Nenjiang River Basin in China as study region and simulated temperature change based on land cover change from 1950s to 1978 and from 1978 to 2010. The analysis of temperature difference sensitivity to land cover change based on Weather Research and Forecasting (WRF) model showed that the land cover change from 1950s to 1978 induced warming effect over all the study area, including the change of grassland to agriculture land, grassland to deciduous broad-leaved forest, and deciduous broad-leaved forest to shrub land. The land cover change from 1978 to 2010 induced cooling effect over all the study area, including the change of deciduous broad-leaved forest to agriculture land, grassland to agriculture land, shrub land to agriculture land, and deciduous broad-leaved forest to grassland. In addition, the warming and cooling effect of land cover change was more significant in the region scale than specific land cover change area.

  17. A comparative analysis of the Global Land Cover 2000 and MODIS land cover data sets

    USGS Publications Warehouse

    Giri, C.; Zhu, Z.; Reed, B.

    2005-01-01

    Accurate and up-to-date global land cover data sets are necessary for various global change research studies including climate change, biodiversity conservation, ecosystem assessment, and environmental modeling. In recent years, substantial advancement has been achieved in generating such data products. Yet, we are far from producing geospatially consistent high-quality data at an operational level. We compared the recently available Global Land Cover 2000 (GLC-2000) and MODerate resolution Imaging Spectrometer (MODIS) global land cover data to evaluate the similarities and differences in methodologies and results, and to identify areas of spatial agreement and disagreement. These two global land cover data sets were prepared using different data sources, classification systems, and methodologies, but using the same spatial resolution (i.e., 1 km) satellite data. Our analysis shows a general agreement at the class aggregate level except for savannas/shrublands, and wetlands. The disagreement, however, increases when comparing detailed land cover classes. Similarly, percent agreement between the two data sets was found to be highly variable among biomes. The identified areas of spatial agreement and disagreement will be useful for both data producers and users. Data producers may use the areas of spatial agreement for training area selection and pay special attention to areas of disagreement for further improvement in future land cover characterization and mapping. Users can conveniently use the findings in the areas of agreement, whereas users might need to verify the informaiton in the areas of disagreement with the help of secondary information. Learning from past experience and building on the existing infrastructure (e.g., regional networks), further research is necessary to (1) reduce ambiguity in land cover definitions, (2) increase availability of improved spatial, spectral, radiometric, and geometric resolution satellite data, and (3) develop advanced

  18. An overview of high temperature metal fatigue: Aspects covered by the 1973 International Conference on Creep and Fatigue

    NASA Technical Reports Server (NTRS)

    Manson, S. S.; Halford, G. R.

    1974-01-01

    A summary of papers is presented which covers the following broad aspects of high temperature metal fatigue: (1) materials development and characterization; (2) environmental factors, including air, vacuum, helium, iodine, sodium, and radiation environments; (3) general fatigue life relationships; (4) crack growth laws; (5) design code activities; and (6) design and service experience. Illustrative tables accompany the summary.

  19. Vocational Cooperative Education Training Agreement and Training Plan for Pennsylvania Including: Training Activities for Use on Training Plans Which Are Listed with Occupational Codes and Titles--Trade & Industrial, Technical, Health, Agriculture, Gainful Home Economics, Business Education, Distributive Education.

    ERIC Educational Resources Information Center

    Millersville State Coll., PA.

    This report on vocational training agreements and plans in Pennsylvania presents a uniform and systematic approach to formalize the relationships between vocational educators and employers in providing students with on-the-job training. First, the report briefly covers the purpose of formalizing on-the-job learning experiences, training agreement,…

  20. III-nitride monolithic LED covering full RGB color gamut

    NASA Astrophysics Data System (ADS)

    El-Ghoroury, Hussein S.; Chuang, Chih-Li; Kisin, Mikhail V.

    2016-03-01

    We present numerical simulation of III-nitride monolithic multi-color LED covering full red-green-blue (RGB) color gamut. The RGB LED structure was grown at Ostendo Technologies Inc. and has been used in Ostendo proprietary Quantum Photonic Imager (QPI) device. Active region of our RGB LED incorporates specially designed intermediate carrier blocking layers (ICBLs) controlling transport of each type of carriers and subsequent carrier injection redistribution among the optically active quantum wells (QWs) with different emission wavelengths. ICBLs are proved to be essential elements of multi-color LED active region design requiring optimization both in material composition and doping level. Strong interdependence between ICBL parameters and active QW characteristics presents additional challenge to multi-color LED design. Combination of several effects was crucial for adequate simulation of RGB LED color control features. Standard drift-diffusion transport model has been appended with rate equations for dynamic QW-confined carrier populations which appear severely off-balanced from corresponding mobile carrier subsystems. QW overshoot and Auger-assisted QW depopulation were also included into the carrier kinetic model thus enhancing the non-equilibrium character of QW confined populations and supporting the mobile carrier transport across the MQW active region. For device simulation we use COMSOL-based program suit developed at Ostendo Technologies Inc.

  1. Corrugated cover plate for flat plate collector

    DOEpatents

    Hollands, K. G. Terry; Sibbitt, Bruce

    1978-01-01

    A flat plate radiant energy collector is providing having a transparent cover. The cover has a V-corrugated shape which reduces the amount of energy reflected by the cover away from the flat plate absorber of the collector.

  2. Heritage Adoption Lessons Learned: Cover Deployment and Latch Mechanism

    NASA Technical Reports Server (NTRS)

    Wincentsen, James

    2006-01-01

    This viewgraph presentation reviews the lessons learned from heritage adoption designs. A general overview of cover deployment hardware that includes the three mechanisms of latch, hinge, and energy absorbers are also discussed.

  3. Covers by polars of arrangements

    SciTech Connect

    Eaves, B.C. . Systems Optimization Lab.); Hoffman, A.J. )

    1990-08-01

    For a collection of hyperplanes passing through the origin in euclidean space let S be the induced subdivision. Let T be the collection of polars of the full cells of S. If only the origin lies in all hyperplanes, T forms a {kappa} {approximately} fold cover of euclidean space. If, in addition, the collection of hyperplanes is of size m and is regular, then {kappa} is m {minus} 1 choose n {minus} 1. Other similar results relate to spheres, hemispheres, and linear programming.

  4. Zebrafish homologs of genes within 16p11.2, a genomic region associated with brain disorders, are active during brain development, and include two deletion dosage sensor genes.

    PubMed

    Blaker-Lee, Alicia; Gupta, Sunny; McCammon, Jasmine M; De Rienzo, Gianluca; Sive, Hazel

    2012-11-01

    Deletion or duplication of one copy of the human 16p11.2 interval is tightly associated with impaired brain function, including autism spectrum disorders (ASDs), intellectual disability disorder (IDD) and other phenotypes, indicating the importance of gene dosage in this copy number variant region (CNV). The core of this CNV includes 25 genes; however, the number of genes that contribute to these phenotypes is not known. Furthermore, genes whose functional levels change with deletion or duplication (termed 'dosage sensors'), which can associate the CNV with pathologies, have not been identified in this region. Using the zebrafish as a tool, a set of 16p11.2 homologs was identified, primarily on chromosomes 3 and 12. Use of 11 phenotypic assays, spanning the first 5 days of development, demonstrated that this set of genes is highly active, such that 21 out of the 22 homologs tested showed loss-of-function phenotypes. Most genes in this region were required for nervous system development - impacting brain morphology, eye development, axonal density or organization, and motor response. In general, human genes were able to substitute for the fish homolog, demonstrating orthology and suggesting conserved molecular pathways. In a screen for 16p11.2 genes whose function is sensitive to hemizygosity, the aldolase a (aldoaa) and kinesin family member 22 (kif22) genes were identified as giving clear phenotypes when RNA levels were reduced by ∼50%, suggesting that these genes are deletion dosage sensors. This study leads to two major findings. The first is that the 16p11.2 region comprises a highly active set of genes, which could present a large genetic target and might explain why multiple brain function, and other, phenotypes are associated with this interval. The second major finding is that there are (at least) two genes with deletion dosage sensor properties among the 16p11.2 set, and these could link this CNV to brain disorders such as ASD and IDD. PMID:22566537

  5. Use of Sentinels to aid the global monitoring of snow cover

    NASA Astrophysics Data System (ADS)

    Pulliainen, Jouni; Salminen, Miia; Luojus, Kari; Metsämäki, Sari; Lemmetyinen, Juha; Takala, Matias; Cohen, Juval; Böttcher, Kristine

    2014-05-01

    Earth observation instruments onboard Sentinel satellites provide a unique opportunity for the monitoring and investigation of global snow processes. The issue of the possible decay of seasonal snow cover is highly relevant for climate research. In addition to water cycle, the extent and amount of snow affects to surface albedo, and indirectly to carbon cycling. The latter issue includes snow-induced changes in permafrost regions (active layer characteristics), as well as the effect of snow (melt) to vegetation growth and soil respiration. Recent advances in ESA DUE GlobSnow project have shown that by combining data from optical satellite sensors and passive microwave instruments advanced Climate Data Records (CDR) on seasonal snow cover can be produced, extending to time periods of over 30 years. The combined snow cover products provide information both on Snow Extent (SE) and Snow Water Equivalent (SWE) on a daily basis. The applicable instruments providing historical data for CDR generation include such microwave radiometers as SMMR, AMSR and SSMI/I, and such optical sensors as AVHRR, AATSR and VIIRS. Sentinel 3, especially its SLSTR instrument, is a prominent tool for expanding the snow CDR for forthcoming years. The developed global snow cover monitoring methodology, demonstrated and discussed here, derives the SWE information from passive microwave data (accompanied with in situ observations of snow depth at synoptic weather stations). The snow extent and fractional snow cover (FSC) on ground is derived from optical satellite data, in order to accurately map the continental line of seasonal snow cover, and to map regions of ephemeral snow cover. An advanced feature in the developed methodology is the provision of uncertainty information on snow cover characteristics associated with each individual satellite data footprint on ground and moment of time. In addition to assisting the generation and extension of the global snow cover CDR, Sentinel missions provide

  6. Modeled impact of anthropogenic land cover change on climate

    USGS Publications Warehouse

    Findell, K.L.; Shevliakova, E.; Milly, P.C.D.; Stouffer, R.J.

    2007-01-01

    Equilibrium experiments with the Geophysical Fluid Dynamics Laboratory's climate model are used to investigate the impact of anthropogenic land cover change on climate. Regions of altered land cover include large portions of Europe, India, eastern China, and the eastern United States. Smaller areas of change are present in various tropical regions. This study focuses on the impacts of biophysical changes associated with the land cover change (albedo, root and stomatal properties, roughness length), which is almost exclusively a conversion from forest to grassland in the model; the effects of irrigation or other water management practices and the effects of atmospheric carbon dioxide changes associated with land cover conversion are not included in these experiments. The model suggests that observed land cover changes have little or no impact on globally averaged climatic variables (e.g., 2-m air temperature is 0.008 K warmer in a simulation with 1990 land cover compared to a simulation with potential natural vegetation cover). Differences in the annual mean climatic fields analyzed did not exhibit global field significance. Within some of the regions of land cover change, however, there are relatively large changes of many surface climatic variables. These changes are highly significant locally in the annual mean and in most months of the year in eastern Europe and northern India. They can be explained mainly as direct and indirect consequences of model-prescribed increases in surface albedo, decreases in rooting depth, and changes of stomatal control that accompany deforestation. ?? 2007 American Meteorological Society.

  7. Monitoring Land Cover Dynamics at Varying Spatial Scales Using High to Very High Resolution Optical Imagery

    NASA Astrophysics Data System (ADS)

    Lavender, S. J.

    2016-06-01

    Activities have focused on using the Landsat time-series and Sentinel-2 datasets to monitor land cover dynamics across the United Kingdom, with mapping of specific areas including missions such as Worldview and Kompsat. This short conference paper shows some of the preliminary results from the Landsat Operational Land Imager, Thematic Mapper and Enhanced Thematic Mapper data processing that has included the development of a pre-processing system that includes cloud masking and an atmospheric correction. The results are promising, but further research is needed.

  8. Monitoring Areal Snow Cover Using NASA Satellite Imagery

    NASA Technical Reports Server (NTRS)

    Harshburger, Brian J.; Blandford, Troy; Moore, Brandon

    2011-01-01

    The objective of this project is to develop products and tools to assist in the hydrologic modeling process, including tools to help prepare inputs for hydrologic models and improved methods for the visualization of streamflow forecasts. In addition, this project will facilitate the use of NASA satellite imagery (primarily snow cover imagery) by other federal and state agencies with operational streamflow forecasting responsibilities. A GIS software toolkit for monitoring areal snow cover extent and producing streamflow forecasts is being developed. This toolkit will be packaged as multiple extensions for ArcGIS 9.x and an opensource GIS software package. The toolkit will provide users with a means for ingesting NASA EOS satellite imagery (snow cover analysis), preparing hydrologic model inputs, and visualizing streamflow forecasts. Primary products include a software tool for predicting the presence of snow under clouds in satellite images; a software tool for producing gridded temperature and precipitation forecasts; and a suite of tools for visualizing hydrologic model forecasting results. The toolkit will be an expert system designed for operational users that need to generate accurate streamflow forecasts in a timely manner. The Remote Sensing of Snow Cover Toolbar will ingest snow cover imagery from multiple sources, including the MODIS Operational Snowcover Data and convert them to gridded datasets that can be readily used. Statistical techniques will then be applied to the gridded snow cover data to predict the presence of snow under cloud cover. The toolbar has the ability to ingest both binary and fractional snow cover data. Binary mapping techniques use a set of thresholds to determine whether a pixel contains snow or no snow. Fractional mapping techniques provide information regarding the percentage of each pixel that is covered with snow. After the imagery has been ingested, physiographic data is attached to each cell in the snow cover image. This data

  9. Regional Differences in the Association Between Land Cover and West Nile Virus Disease Incidence in Humans in the United States

    PubMed Central

    Bowden, Sarah E.; Magori, Krisztian; Drake, John M.

    2011-01-01

    West Nile virus (WNV) is generally considered to be an urban pathogen in the United States, but studies associating land cover and disease incidence, seroprevalence, or infection rate in humans, birds, domesticated and wild mammals, and mosquitoes report varying and sometimes contradictory results at an array of spatial extents. Human infection can provide insight about basic transmission activity; therefore, we analyzed data on the incidence of WNV disease in humans to obtain a comprehensive picture of how human disease and land cover type are associated across the United States. Human WNV disease incidence in Northeastern regions was positively associated with urban land covers, whereas incidence in the Western United States was positively associated with agricultural land covers. We suggest that these regional associations are explained by the geographic distributions of prominent WNV vectors: Culex pipiens complex (including Cx. pipiens and Cx. quinquefasciatus) in the Northeast and Cx. tarsalis in the Western United States. PMID:21292890

  10. Regional differences in the association between land cover and West Nile virus disease incidence in humans in the United States.

    PubMed

    Bowden, Sarah E; Magori, Krisztian; Drake, John M

    2011-02-01

    West Nile virus (WNV) is generally considered to be an urban pathogen in the United States, but studies associating land cover and disease incidence, seroprevalence, or infection rate in humans, birds, domesticated and wild mammals, and mosquitoes report varying and sometimes contradictory results at an array of spatial extents. Human infection can provide insight about basic transmission activity; therefore, we analyzed data on the incidence of WNV disease in humans to obtain a comprehensive picture of how human disease and land cover type are associated across the United States. Human WNV disease incidence in Northeastern regions was positively associated with urban land covers, whereas incidence in the Western United States was positively associated with agricultural land covers. We suggest that these regional associations are explained by the geographic distributions of prominent WNV vectors: Culex pipiens complex (including Cx. pipiens and Cx. quinquefasciatus) in the Northeast and Cx. tarsalis in the Western United States.

  11. The Middeck Active Control Experiment (MACE)

    NASA Technical Reports Server (NTRS)

    Miller, David W.

    1991-01-01

    Viewgraphs on the Middeck Active Control Experiment (MACE) are presented. Topics covered include: science program objectives and rationale; science requirements; capturing the essential physics; science development approach; development model hardware; development model test plan; and flight hardware and operations.

  12. Zebrafish homologs of genes within 16p11.2, a genomic region associated with brain disorders, are active during brain development, and include two deletion dosage sensor genes

    PubMed Central

    Blaker-Lee, Alicia; Gupta, Sunny; McCammon, Jasmine M.; De Rienzo, Gianluca; Sive, Hazel

    2012-01-01

    SUMMARY Deletion or duplication of one copy of the human 16p11.2 interval is tightly associated with impaired brain function, including autism spectrum disorders (ASDs), intellectual disability disorder (IDD) and other phenotypes, indicating the importance of gene dosage in this copy number variant region (CNV). The core of this CNV includes 25 genes; however, the number of genes that contribute to these phenotypes is not known. Furthermore, genes whose functional levels change with deletion or duplication (termed ‘dosage sensors’), which can associate the CNV with pathologies, have not been identified in this region. Using the zebrafish as a tool, a set of 16p11.2 homologs was identified, primarily on chromosomes 3 and 12. Use of 11 phenotypic assays, spanning the first 5 days of development, demonstrated that this set of genes is highly active, such that 21 out of the 22 homologs tested showed loss-of-function phenotypes. Most genes in this region were required for nervous system development – impacting brain morphology, eye development, axonal density or organization, and motor response. In general, human genes were able to substitute for the fish homolog, demonstrating orthology and suggesting conserved molecular pathways. In a screen for 16p11.2 genes whose function is sensitive to hemizygosity, the aldolase a (aldoaa) and kinesin family member 22 (kif22) genes were identified as giving clear phenotypes when RNA levels were reduced by ∼50%, suggesting that these genes are deletion dosage sensors. This study leads to two major findings. The first is that the 16p11.2 region comprises a highly active set of genes, which could present a large genetic target and might explain why multiple brain function, and other, phenotypes are associated with this interval. The second major finding is that there are (at least) two genes with deletion dosage sensor properties among the 16p11.2 set, and these could link this CNV to brain disorders such as ASD and IDD. PMID

  13. Toward the standard population synthesis model of the X-ray background: Evolution of X-ray luminosity and absorption functions of active galactic nuclei including Compton-thick populations

    SciTech Connect

    Ueda, Yoshihiro; Akiyama, Masayuki; Hasinger, Günther; Miyaji, Takamitsu; Watson, Michael G.

    2014-05-10

    We present the most up to date X-ray luminosity function (XLF) and absorption function of active galactic nuclei (AGNs) over the redshift range from 0 to 5, utilizing the largest, highly complete sample ever available obtained from surveys performed with Swift/BAT, MAXI, ASCA, XMM-Newton, Chandra, and ROSAT. The combined sample, including that of the Subaru/XMM-Newton Deep Survey, consists of 4039 detections in the soft (0.5-2 keV) and/or hard (>2 keV) band. We utilize a maximum likelihood method to reproduce the count rate versus redshift distribution for each survey, by taking into account the evolution of the absorbed fraction, the contribution from Compton-thick (CTK) AGNs, and broadband spectra of AGNs, including reflection components from tori based on the luminosity- and redshift-dependent unified scheme. We find that the shape of the XLF at z ∼ 1-3 is significantly different from that in the local universe, for which the luminosity-dependent density evolution model gives much better description than the luminosity and density evolution model. These results establish the standard population synthesis model of the X-ray background (XRB), which well reproduces the source counts, the observed fractions of CTK AGNs, and the spectrum of the hard XRB. The number ratio of CTK AGNs to the absorbed Compton-thin (CTN) AGNs is constrained to be ≈0.5-1.6 to produce the 20-50 keV XRB intensity within present uncertainties, by assuming that they follow the same evolution as CTN AGNs. The growth history of supermassive black holes is discussed based on the new AGN bolometric luminosity function.

  14. Land cover changes associated with recent energy development in the Williston Basin; Northern Great Plains, USA

    USGS Publications Warehouse

    Preston, Todd M.; Kim, Kevin

    2016-01-01

    The Williston Basin in the Northern Great Plains has experienced rapid energy development since 2000. To evaluate the land cover changes resulting from recent (2000 – 2015) development, the area and previous land cover of all well pads (pads) constructed during this time was determined, the amount of disturbed and reclaimed land adjacent to pads was estimated, land cover changes were analyzed over time for three different well types, and the effects from future development were predicted. The previous land cover of the 12,990 ha converted to pads was predominately agricultural (49.5%) or prairie (47.4%) with lesser amounts of developed (2.3%), aquatic (0.5%), and forest (0.4%). Additionally, 12,121 ha have likely been disturbed and reclaimed. The area required per gas well remained constant through time while the land required per oil well increased initially and then decreased as development first shifted from conventional to unconventional drilling and then to multi-bore pads. For non-oil-and- gas wells (i.e. stratigraphic test wells, water wells, injection wells, etc.), the area per well increased through time likely due to increased produced water disposal requirements. Future land cover change is expected to be 2.7 times greater than recent development with much of the development occurring in five counties in the core Bakken development area. Direct land cover change and disturbance from recent and expected development are predicted to affect 0.4% of the landscape across the basin; however, in the core Bakken development area, 2.3% of the landscape will be affected including 2.1% of the remaining grassland. Although future development will result in significant land cover change, evolving industry practices and proactive siting decisions, such as development along energy corridors and placing pads in areas previously altered by human activity, have the potential to reduce the ecological effects of future energy development in the Williston Basin.

  15. Land cover changes associated with recent energy development in the Williston Basin; Northern Great Plains, USA.

    PubMed

    Preston, Todd M; Kim, Kevin

    2016-10-01

    The Williston Basin in the Northern Great Plains has experienced rapid energy development since 2000. To evaluate the land cover changes resulting from recent (2000-2015) development, the area and previous land cover of all well pads (pads) constructed during this time were determined, the amount of disturbed and reclaimed land adjacent to pads was estimated, land cover changes were analyzed over time for three different well types, and the effects from future development were predicted. The previous land cover of the 12,990ha converted to pads was predominately agricultural (49.5%) or prairie (47.4%) with lesser amounts of developed (2.3%), aquatic (0.5%), and forest (0.4%). Additionally, 12,121ha has likely been disturbed and reclaimed. The area required per gas well remained constant through time while the land required per oil well increased initially and then decreased as development first shifted from conventional to unconventional drilling and then to multi-bore pads. For non-oil-and-gas wells (i.e. stratigraphic test wells, water wells, and injection wells), the area per well increased through time likely due to increased produced water disposal requirements. Future land cover change is expected to be 2.7 times greater than recent development with much of the development occurring in five counties in the core Bakken development area. Direct land cover change and disturbance from recent and expected development are predicted to affect 0.4% of the landscape across the basin; however, in the core Bakken development area, 2.3% of the landscape will be affected including 2.1% of the remaining grassland. Although future development will result in significant land cover change, evolving industry practices and proactive siting decisions, such as development along energy corridors and placing pads in areas previously altered by human activity, have the potential to reduce the ecological effects of future energy development in the Williston Basin. PMID:27318516

  16. Land cover changes associated with recent energy development in the Williston Basin; Northern Great Plains, USA.

    PubMed

    Preston, Todd M; Kim, Kevin

    2016-10-01

    The Williston Basin in the Northern Great Plains has experienced rapid energy development since 2000. To evaluate the land cover changes resulting from recent (2000-2015) development, the area and previous land cover of all well pads (pads) constructed during this time were determined, the amount of disturbed and reclaimed land adjacent to pads was estimated, land cover changes were analyzed over time for three different well types, and the effects from future development were predicted. The previous land cover of the 12,990ha converted to pads was predominately agricultural (49.5%) or prairie (47.4%) with lesser amounts of developed (2.3%), aquatic (0.5%), and forest (0.4%). Additionally, 12,121ha has likely been disturbed and reclaimed. The area required per gas well remained constant through time while the land required per oil well increased initially and then decreased as development first shifted from conventional to unconventional drilling and then to multi-bore pads. For non-oil-and-gas wells (i.e. stratigraphic test wells, water wells, and injection wells), the area per well increased through time likely due to increased produced water disposal requirements. Future land cover change is expected to be 2.7 times greater than recent development with much of the development occurring in five counties in the core Bakken development area. Direct land cover change and disturbance from recent and expected development are predicted to affect 0.4% of the landscape across the basin; however, in the core Bakken development area, 2.3% of the landscape will be affected including 2.1% of the remaining grassland. Although future development will result in significant land cover change, evolving industry practices and proactive siting decisions, such as development along energy corridors and placing pads in areas previously altered by human activity, have the potential to reduce the ecological effects of future energy development in the Williston Basin.

  17. Regional characterization of land cover using multiple sources of data

    USGS Publications Warehouse

    Vogelmann, J.E.; Sohl, T.; Howard, S.M.

    1998-01-01

    Many organizations require accurate intermediate-scale land-cover information for many applications, including modeling nutrient and pesticide runoff, understanding spatial patterns of biodiversity, land-use planning, and policy development. While many techniques have been successfully used to classify land cover in relatively small regions, there are substantial obstacles in applying these methods to large, multiscene regions. The purpose of this study was to generate and evaluate a large region land-cover classification product using a multiple-layer land-characteristics database approach. To derive land-cover information, mosaicked Landsat thematic mapper (TM) scenes were analyzed in conjunction with digital elevation data (and derived slope, aspect, and shaded relief), population census information, Defense Meteorological Satellite Program city lights data, prior land-use and land-cover data, digital line graph data, and National Wetlands Inventory data. Both leaf-on and leaf-off TM data sets were analyzed. The study area was U.S. Federal Region III, which includes the states of Pennsylvania, Virginia, Maryland, Delaware, and West Virginia. The general procedure involved (1) generating mosaics of multiple scenes of leaves-on TM data using histogram equalization methods; (2) clustering mosaics into 100 spectral classes using unsupervised classification; (3) interpreting and labeling spectral classes into approximately 15 land-cover categories (analogous to Anderson Level 1 and 2 classes) using aerial photographs; (4) developing decision-making rules and models using from one to several ancillary data layers to resolve confusion in spectral classes that represented two or more targeted land-cover categories; and (5) incorporating data from other sources (for example, leaf-off TM data and National Wetlands Inventory data) to yield a final land-cover product. Although standard accuracy assessments were not done, a series of consistency checks using available

  18. Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells.

    PubMed

    Lammi, Carmen; Zanoni, Chiara; Ferruzza, Simonetta; Ranaldi, Giulia; Sambuy, Yula; Arnoldi, Anna

    2016-01-01

    Literature indicates that peptic and tryptic peptides derived from the enzymatic hydrolysis of lupin protein are able to modulate cholesterol metabolism in human hepatic HepG2 cells and that part of these peptides are absorbed in a small intestine model based on differentiated human Caco-2 cells. In this paper, a co-culture system, including Caco-2 and HepG2 cells, was investigated with two objectives: (a) to verify whether cholesterol metabolism in HepG2 cells was modified by the peptides absorption through Caco-2 cells; (b) to investigate how lupin peptides influence cholesterol metabolism in Caco-2 cells. The experiments showed that the absorbed peptides, not only maintained their bioactivity on HepG2 cells, but that this activity was improved by the crosstalk of the two cells systems in co-culture. In addition, lupin peptides showed a positive influence on cholesterol metabolism in Caco-2 cells, decreasing the proprotein convertase subtilisin/kexin type 9 (PCSK9) secretion. PMID:27455315

  19. Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells

    PubMed Central

    Lammi, Carmen; Zanoni, Chiara; Ferruzza, Simonetta; Ranaldi, Giulia; Sambuy, Yula; Arnoldi, Anna

    2016-01-01

    Literature indicates that peptic and tryptic peptides derived from the enzymatic hydrolysis of lupin protein are able to modulate cholesterol metabolism in human hepatic HepG2 cells and that part of these peptides are absorbed in a small intestine model based on differentiated human Caco-2 cells. In this paper, a co-culture system, including Caco-2 and HepG2 cells, was investigated with two objectives: (a) to verify whether cholesterol metabolism in HepG2 cells was modified by the peptides absorption through Caco-2 cells; (b) to investigate how lupin peptides influence cholesterol metabolism in Caco-2 cells. The experiments showed that the absorbed peptides, not only maintained their bioactivity on HepG2 cells, but that this activity was improved by the crosstalk of the two cells systems in co-culture. In addition, lupin peptides showed a positive influence on cholesterol metabolism in Caco-2 cells, decreasing the proprotein convertase subtilisin/kexin type 9 (PCSK9) secretion. PMID:27455315

  20. Covering the Plane with Rep-Tiles.

    ERIC Educational Resources Information Center

    Fosnaugh, Linda S.; Harrell, Marvin E.

    1996-01-01

    Presents an activity in which students use geometric figures, rep-tiles, to design a tile floor. Rep-tiles are geometric figures of which copies can fit together to form a larger similar figure. Includes reproducible student worksheet. (MKR)

  1. Influence of cover defects on the attenuation of radon with earthen covers

    SciTech Connect

    Kalkwarf, D.R.; Mayer, D.W.

    1983-11-01

    Experimental and theoretical evaluations of radon flux through laboratory-scale defective soil columns are presented together with a survey of literature on the formation and prevention of defects in soil covers. This report focuses on air-filled, centimeter-scale defects that are most probable in earthen covers for attenuating radon emission from uranium-mill tailings. Examples include shirnkage and erosion cracks, erosion piping, animal burrows and air channels formed by the biodegradation of vegetation roots. Calculations based on mathematical models indicate that collections of defects which could increase the radon flux from an earthen cover by a factor of two would be easily detected by visual inspection. However, these models ignore air-turbulence in the defect and drying of the soil around the defect. Laboratory measurements showed that turbulent diffusion of radon occurred through defects as narrow as 0.3 cm when subjected to a transverse air velocity of 1 to 6 miles per hour at the surface. Both turbulence and more-rapid drying of soil can accelerate radon flux to the cover surface. Consequently, recommended methods to inhibit defect formation should be applied. 29 references, 3 figures, 5 tables.

  2. Radon attenuation handbook for uranium mill tailings cover design

    SciTech Connect

    Rogers, V.C.; Nielson, K.K.; Kalkwarf, D.R.

    1984-04-01

    This handbook has been prepared to facilitate the design of earthen covers to control radon emission from uranium mill tailings. Radon emissions from bare and covered uranium mill tailings can be estimated from equations based on diffusion theory. Basic equations are presented for calculating surface radon fluxes from covered tailings, or alternately, the cover thicknesses required to satisfy a given radon flux criterion. Also described is a computer code, RAECOM, for calculating cover thicknesses and surface fluxes. Methods are also described for measuring diffusion coefficients for radon, or for estimating them from empirical correlations. Since long-term soil moisture content is a critical parameter in determining the value of the diffusion coefficient, methods are given for estimating the long-term moisture contents of soils. The effects of cover defects or advection are also discussed and guidelines are given for determining if they are significant. For most practical cases, advection and cover defect effects on radon flux can be neglected. Several examples are given to demonstrate cover design calculations, and an extensive list of references is included. 63 references, 18 figures, 6 tables.

  3. Are We Capturing the True Impacts of Anthropogenic Land Cover Forcings in ESMs?

    NASA Astrophysics Data System (ADS)

    Feddema, J. J.

    2014-12-01

    Simulation of anthropogenically induced land cover change impacts on climate has made significant progress over the last half century, starting with impacts of albedo and land cover change and evolving to include irrigation, urbanization and wood harvest carbon cycle impacts. However, many models still simulate these processes piece meal and the historical and Integrated Assessment Model (IAM) derived datasets used to drive these processes are not necessarily internally consistent in their implementation within Earth System Models. A further important question considers how anthropogenically driven feedbacks in the LULC-climate system will take place. In the future land cover change may be small given the lack of available arable land. However, land use intensification and political actions on energy consumption in urban systems may result in significant new indirect impacts on nutrient cycles and biogeophysical and biogeochemical processes.This paper will review some of the land cover and land use (LCLU) processes simulated in ESMs and a review the sources of information used to define the geographical extent of LULC changes. One question to address is whether the current representation of these processes are adequate to simulate the totality of the anthropogenic climate impacts, given that in many cases there are differences in the intensity of land uses over space and time, and that a particular land cover class may actually encompass a wide variety of human activities. There also remain some land cover types/processes that are not well simulated in most models. For example the ill-defined land use class associated with pasture or grazing activities. Finally, it is important to consider which land cover types are most likely to change in the future. Perhaps more important than the spatial change is how processes within land cover types will change. For example, urban systems are likely to play a major role in determining LULC related influences on anthropogenic

  4. Methods for Cloud Cover Estimation

    NASA Technical Reports Server (NTRS)

    Glackin, D. L.; Huning, J. R.; Smith, J. H.; Logan, T. L.

    1984-01-01

    Several methods for cloud cover estimation are described relevant to assessing the performance of a ground-based network of solar observatories. The methods rely on ground and satellite data sources and provide meteorological or climatological information. One means of acquiring long-term observations of solar oscillations is the establishment of a ground-based network of solar observatories. Criteria for station site selection are: gross cloudiness, accurate transparency information, and seeing. Alternative methods for computing this duty cycle are discussed. The cycle, or alternatively a time history of solar visibility from the network, can then be input to a model to determine the effect of duty cycle on derived solar seismology parameters. Cloudiness from space is studied to examine various means by which the duty cycle might be computed. Cloudiness, and to some extent transparency, can potentially be estimated from satellite data.

  5. TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets.

    PubMed

    Xue, Gang-Ping; Drenth, Janneke; McIntyre, C Lynne

    2015-02-01

    Heat stress is a significant environmental factor adversely affecting crop yield. Crop adaptation to high-temperature environments requires transcriptional reprogramming of a suite of genes involved in heat stress protection. This study investigated the role of TaHsfA6f, a member of the A6 subclass of heat shock transcription factors, in the regulation of heat stress protection genes in Triticum aestivum (bread wheat), a poorly understood phenomenon in this crop species. Expression analysis showed that TaHsfA6f was expressed constitutively in green organs but was markedly up-regulated during heat stress. Overexpression of TaHsfA6f in transgenic wheat using a drought-inducible promoter resulted in up-regulation of heat shock proteins (HSPs) and a number of other heat stress protection genes that included some previously unknown Hsf target genes such as Golgi anti-apoptotic protein (GAAP) and the large isoform of Rubisco activase. Transgenic wheat plants overexpressing TaHsfA6f showed improved thermotolerance. Transactivation assays showed that TaHsfA6f activated the expression of reporter genes driven by the promoters of several HSP genes (TaHSP16.8, TaHSP17, TaHSP17.3, and TaHSP90.1-A1) as well as TaGAAP and TaRof1 (a co-chaperone) under non-stress conditions. DNA binding analysis revealed the presence of high-affinity TaHsfA6f-binding heat shock element-like motifs in the promoters of these six genes. Promoter truncation and mutagenesis analyses identified TaHsfA6f-binding elements that were responsible for transactivation of TaHSP90.1-A1 and TaGAAP by TaHsfA6f. These data suggest that TaHsfA6f is a transcriptional activator that directly regulates TaHSP, TaGAAP, and TaRof1 genes in wheat and its gene regulatory network has a positive impact on thermotolerance.

  6. Complications associated with use of tantalum-mesh--covered implants.

    PubMed

    Przybyla, V A; La Piana, F G

    1982-02-01

    In 1948, ophthalmologists started implanting plastic spheres partially covered with tantalum mesh (eg, the Valley Forge Implant) in anophthalmic sockets. Numerous patients have developed complications ten to 15 years after surgery: pain ("pinching" or "deep"), headache, heavy mucopurulent discharge and diffuse conjunctival inflammation, migration of the implant, thinning or erosion of tissues covering the implant (extrusion), and inability to wear an artificial eye. Management includes refabrication of the artificial eye and replacement of the implant with a dermis-fat graft or a silicone ball plus scleral graft. Tantalum-mesh--covered orbital implants should probably not be used.

  7. Detection of covered materials in the TDS-THz setup

    NASA Astrophysics Data System (ADS)

    Palka, Norbert

    2013-05-01

    We report on a new method for extracting the characteristic features of covered materials, including Hexogen, in the range 0.5-1.8 THz. This time domain spectroscopy-based technique takes into account only part of the signal reflected from a covered sample, and analyzes it by Fourier transform. The obtained power spectrum has distinctive peaks that correspond to peaks measured in the transmission configuration and can be applied for further identification. We showed results obtained for the samples of hexogen, lactose, and tartaric acid covered with commonly used packaging materials such as plastic, foil, paper and cotton.

  8. Activities.

    ERIC Educational Resources Information Center

    Kincaid, Charlene; And Others

    1993-01-01

    Presents an activity in which students collect and organize data from a real-world simulation of the scientific concept of half life. Students collect data using a marble sifter, analyze the data using a graphing calculator, and determine an appropriate mathematical model. Includes reproducible worksheets. (MDH)

  9. Construction Costs of Six Landfill Cover Designs

    SciTech Connect

    Dwyer, S.F.

    1998-12-23

    A large-scale field demonstration comparing and contrasting final landfill cover designs has been constructed and is currently being monitored. Four alternative cover designs and two conventional designs (a RCRA Subtitle `D' Soil Cover and a RCRA Subtitle `C' Compacted Clay Cover) were constructed side-by-side for direct comparison. The demonstration is intended to evaluate the various cover designs based on their respective water balance performance, ease and reliability of construction, and cost. This paper provides an overview of the construction costs of each cover design.

  10. Shuttle landing facility cloud cover study: Climatological analysis and two tenths cloud cover rule evaluation

    NASA Technical Reports Server (NTRS)

    Atchison, Michael K.; Schumann, Robin; Taylor, Greg; Warburton, John; Wheeler, Mark; Yersavich, Ann

    1993-01-01

    The two-tenths cloud cover rule in effect for all End Of Mission (EOM) STS landings at the Kennedy Space Center (KSC) states: 'for scattered cloud layers below 10,000 feet, cloud cover must be observed to be less than or equal to 0.2 at the de-orbit burn go/no-go decision time (approximately 90 minutes before landing time)'. This rule was designed to protect against a ceiling (below 10,000 feet) developing unexpectedly within the next 90 minutes (i.e., after the de-orbit burn decision and before landing). The Applied Meteorological Unit (AMU) developed and analyzed a database of cloud cover amounts and weather conditions at the Shuttle Landing Facility for a five-year (1986-1990) period. The data indicate the best time to land the shuttle at KSC is during the summer while the worst time is during the winter. The analysis also shows the highest frequency of landing opportunities occurs for the 0100-0600 UTC and 1300-1600 UTC time periods. The worst time of the day to land a shuttle is near sunrise and during the afternoon. An evaluation of the two-tenths cloud cover rule for most data categorizations has shown that there is a significant difference in the proportions of weather violations one and two hours subsequent to initial conditions of 0.2 and 0.3 cloud cover. However, for May, Oct., 700 mb northerly wind category, 1500 UTC category, and 1600 UTC category there is some evidence that the 0.2 cloud cover rule may be overly conservative. This possibility requires further investigation. As a result of these analyses, the AMU developed nomograms to help the Spaceflight Meteorological Group (SMG) and the Cape Canaveral Forecast Facility (CCFF) forecast cloud cover for EOM and Return to Launch Site (RTLS) at KSC. Future work will include updating the two tenths database, further analysis of the data for several categorizations, and developing a proof of concept artificial neural network to provide forecast guidance of weather constraint violations for shuttle

  11. Monitoring Final Cover Performance - Findings from a Nationwide Study

    NASA Astrophysics Data System (ADS)

    Benson, C. H.; Albright, W. H.

    2006-05-01

    Final covers are placed over waste containment facilities to provide long-term isolation of the underlying waste. For most covers, the primary function is to limit the amount of water that percolates downward, thereby reducing the amount of leachate that is generated within the waste mass. In 1998, the US Environmental Protection Agency (USEPA) recognized that data describing the hydrologic performance of final covers was scarce, with most of the existing studies being limited to a single location or a limited number of cover designs. The Alternative Cover Assessment Program (ACAP) was initiated by USEPA as a five-year monitoring study to fill this information gap and to provide guidance on final cover design and performance. The cornerstone of ACAP is a network of 28 large-scale test sections simulating a variety of different cover designs. These test sections are located at 12 sites distributed throughout the US to provide geographic and climatic diversity. This presentation will describe the monitoring systems used in each of the ACAP test sections and will review the performance data that were collected. Some of the final covers performed very well, whereas others performed poorly. Those that performed poorly included cover designs currently required for closure of municipal and hazardous waste landfills in the United States. The data also illustrate that development of soil structure and transitions in vegetative species can have a profound impact on the performance of final covers, but information is limited regarding the rate at which these changes occur, their magnitude, and how they depend on the soils and methods used for construction.

  12. Design and Installation of a Disposal Cell Cover Field Test

    SciTech Connect

    Benson, C.H.; Waugh, W.J.; Albright, W.H.; Smith, G.M.; Bush, R.P.

    2011-02-27

    The U.S. Department of Energy’s Office of Legacy Management (LM) initiated a cover assessment project in September 2007 to evaluate an inexpensive approach to enhancing the hydrological performance of final covers for disposal cells. The objective is to accelerate and enhance natural processes that are transforming existing conventional covers, which rely on low-conductivity earthen barriers, into water balance covers, that store water in soil and release it as soil evaporation and plant transpiration. A low conductivity cover could be modified by deliberately blending the upper layers of the cover profile and planting native shrubs. A test facility was constructed at the Grand Junction, Colorado, Disposal Site to evaluate the proposed methodology. The test cover was constructed in two identical sections, each including a large drainage lysimeter. The test cover was constructed with the same design and using the same materials as the existing disposal cell in order to allow for a direct comparison of performance. One test section will be renovated using the proposed method; the other is a control. LM is using the lysimeters to evaluate the effectiveness of the renovation treatment by monitoring hydrologic conditions within the cover profile as well as all water entering and leaving the system. This paper describes the historical experience of final covers employing earthen barrier layers, the design and operation of the lysimeter test facility, testing conducted to characterize the as-built engineering and edaphic properties of the lysimeter soils, the calibration of instruments installed at the test facility, and monitoring data collected since the lysimeters were constructed.

  13. Global land cover mapping: a review and uncertainty analysis

    USGS Publications Warehouse

    Congalton, Russell G.; Gu, Jianyu; Yadav, Kamini; Thenkabail, Prasad S.; Ozdogan, Mutlu

    2014-01-01

    Given the advances in remotely sensed imagery and associated technologies, several global land cover maps have been produced in recent times including IGBP DISCover, UMD Land Cover, Global Land Cover 2000 and GlobCover 2009. However, the utility of these maps for specific applications has often been hampered due to considerable amounts of uncertainties and inconsistencies. A thorough review of these global land cover projects including evaluating the sources of error and uncertainty is prudent and enlightening. Therefore, this paper describes our work in which we compared, summarized and conducted an uncertainty analysis of the four global land cover mapping projects using an error budget approach. The results showed that the classification scheme and the validation methodology had the highest error contribution and implementation priority. A comparison of the classification schemes showed that there are many inconsistencies between the definitions of the map classes. This is especially true for the mixed type classes for which thresholds vary for the attributes/discriminators used in the classification process. Examination of these four global mapping projects provided quite a few important lessons for the future global mapping projects including the need for clear and uniform definitions of the classification scheme and an efficient, practical, and valid design of the accuracy assessment.

  14. Assessing, understanding, and conveying the state of the Arctic sea ice cover

    NASA Astrophysics Data System (ADS)

    Perovich, D. K.; Richter-Menge, J. A.; Rigor, I.; Parkinson, C. L.; Weatherly, J. W.; Nghiem, S. V.; Proshutinsky, A.; Overland, J. E.

    2003-12-01

    Recent studies indicate that the Arctic sea ice cover is undergoing significant climate-induced changes, affecting both its extent and thickness. Satellite-derived estimates of Arctic sea ice extent suggest a reduction of about 3% per decade since 1978. Ice thickness data from submarines suggest a net thinning of the sea ice cover since 1958. Changes (including oscillatory changes) in atmospheric circulation and the thermohaline properties of the upper ocean have also been observed. These changes impact not only the Arctic, but the global climate system and are likely accelerated by such processes as the ice-albedo feedback. It is important to continue and expand long-term observations of these changes to (a) improve the fundamental understanding of the role of the sea ice cover in the global climate system and (b) use the changes in the sea ice cover as an early indicator of climate change. This is a formidable task that spans a range of temporal and spatial scales. Fortunately, there are numerous tools that can be brought to bear on this task, including satellite remote sensing, autonomous buoys, ocean moorings, field campaigns and numerical models. We suggest the integrated and coordinated use of these tools during the International Polar Year to monitor the state of the Arctic sea ice cover and investigate its governing processes. For example, satellite remote sensing provides the large-scale snapshots of such basic parameters as ice distribution, melt zone, and cloud fraction at intervals of half a day to a week. Buoys and moorings can contribute high temporal resolution and can measure parameters currently unavailable from space including ice thickness, internal ice temperature, and ocean temperature and salinity. Field campaigns can be used to explore, in detail, the processes that govern the ice cover. Numerical models can be used to assess the character of the changes in the ice cover and predict their impacts on the rest of the climate system. This work

  15. Enhanced Cover Assessment Project:Soil Manipulation and Revegetation Tests

    SciTech Connect

    Waugh, W. Joseph; Albright, Dr. Bill; Benson, Dr. Craig

    2014-02-01

    The U.S. Department of Energy Office of Legacy Management is evaluating methods to enhance natural changes that are essentially converting conventional disposal cell covers for uranium mill tailings into water balance covers. Conventional covers rely on a layer of compacted clayey soil to limit exhalation of radon gas and percolation of rainwater. Water balance covers rely on a less compacted soil “sponge” to store rainwater, and on soil evaporation and plant transpiration (evapotranspiration) to remove stored water and thereby limit percolation. Over time, natural soil-forming and ecological processes are changing conventional covers by increasing hydraulic conductivity, loosening compaction, and increasing evapotranspiration. The rock armor on conventional covers creates a favorable habitat for vegetation by slowing soil evaporation, increasing soil water storage, and trapping dust and organic matter, thereby providing the water and nutrients needed for plant germination, survival, and sustainable transpiration. Goals and Objectives Our overall goal is to determine if allowing or enhancing these natural changes could improve cover performance and reduce maintenance costs over the long term. This test pad study focuses on cover soil hydrology and ecology. Companion studies are evaluating effects of natural and enhanced changes in covers on radon attenuation, erosion, and biointrusion. We constructed a test cover at the Grand Junction disposal site to evaluate soil manipulation and revegetation methods. The engineering design, construction, and properties of the test cover match the upper three layers of the nearby disposal cell cover: a 1-foot armoring of rock riprap, a 6-inch bedding layer of coarse sand and gravel, and a 2-foot protection layer of compacted fine soil. The test cover does not have a radon barrier—cover enhancement tests leave the radon barrier intact. We tested furrowing and ripping as means for creating depressions parallel to the slope

  16. National Level Assessment of Mangrove Forest Cover in Pakistan

    NASA Astrophysics Data System (ADS)

    Abbas, S.; Qamer, F. M.; Hussain, N.; Saleem, R.; Nitin, K. T.

    2011-09-01

    Mangroves ecosystems consist of inter tidal flora and fauna found in the tropical and subtropical regions of the world. Mangroves forest is a collection of halophytic trees, shrubs, and other plants receiving inputs from regular tidal flushing and from freshwater streams and rivers. A global reduction of 25 % mangroves' area has been observed since 1980 and it is categorized as one of to the most threatened and vulnerable ecosystems of the world. Forest resources in Pakistan are being deteriorating both quantitatively and qualitatively due to anthropogenic activities, climatic v and loose institutional management. According to the FAO (2007), extent of forest cover of Pakistan in 2005 is 1,902,000 ha, which is 2.5% of its total land area. Annual change rate during 2000-2005 was -2.1% which is highest among all the countries in Asia. The Indus delta region contains the world's fifth-largest mangrove forest which provides a range of important ecosystem services, including coastal stabilisation, primary production and provision of nursery habitat for marine fish. Given their ecological importance in coastal settings, mangroves receive special attention in the assessment of conservation efforts and sustainable coastal developments. Coastline of Pakistan is 1050km long shared by the provinces, Sind (350km) and Baluchistan (700 km). The coastline, with typical arid subtropical climate, possesses five significant sites that are blessed with mangroves. In the Sindh province, mangroves are found in the Indus Delta and Sandspit. The Indus Delta is host to the most extensive mangroves areas and extends from Korangi Creek in the West to Sir Creek in the East, whereas Sandspit is a small locality in the West of Karachi city. In the Balochistan province, mangroves are located at three sites, Miani Hor, Kalmat Khor and Jiwani. Contemporary methods of Earth observation sciences are being incorporated as an integral part of environmental assessment related studies in coastal areas

  17. Eolian cover sands: a sedimentologic model and paleoenvironmental implications

    SciTech Connect

    Lea, P.D.

    1985-01-01

    In periglacial areas, accumulations of eolian sand commonly form low-relief blankets without well-developed dunes. Internally, these sandsheet deposits exhibit subhorizontal lamination rather than high-angle cross-bedding. Such cover sands of late-Pleistocene age mantle extensive areas in northern Europe, but have been reported more rarely from North America. The processes by which cover sands, as opposed to dunes, accumulate have not yet been determined conclusively. Wind ripples and sand dunes do not form a continuum; flow separation and avalanching and negligible in the former and vital in the latter. Accretion of a sand patch into a mound sufficient to cause flow separation and dune growth requires a consistently available supply of loose sand. In cover-sand areas, sand may be immobilized prior to dune development by several factors: (1) a sparse vegetation cover, (2) moist ground conditions, (3) snow cover, and (4) a shallow permafrost table and/or an ice-cemented active layer. Detailed sedimentologic studies may allow discrimination among these various controls. The importance of the individual controlling factors can vary seasonally in a given deposit, as well as between deposits in different paleogeographic settings. However, all factors imply more mesic conditions than those associated with many dune deposits. The association of cover sands with paraboloid dunes is also consistent with somewhat moist conditions. The relatively mesic nature of cover sands controls their Pleistocene distribution; they become decreasingly important relative to dunes in maritime-to-continental transects across Alaska and northern Europe.

  18. Covering a Crucible with Metal Containing Channels

    NASA Technical Reports Server (NTRS)

    Grugel, Richard N.

    2006-01-01

    In a procedure that partly resembles the lost-wax casting process, a crucible made of a brittle material (ceramic, quartz, or glass) is covered with a layer of metal containing channels. The metal cover and the channels can serve any or all of several purposes, depending upon the application: Typically, the metal would serve at least partly to reinforce the crucible. The channels could be used as passages for narrow objects that could include thermocouples and heat-transfer strips. Alternatively or in addition, channels could be used as flow paths for liquid or gaseous coolants and could be positioned and oriented for position- or direction-selective cooling. In some cases, the channels could be filled with known gases and sealed so that failure of the crucibles could be indicated by instruments that detect the gases. The process consists of three main steps. In the first step, a pattern defining the channels is formed by wrapping or depositing a material in the desired channel pattern on the outer surface of the crucible. The pattern material can be a plastic, wax, low-ash fibrous material, a soluble material, or other suitable material that can subsequently be removed easily. In a proof-of-concept demonstration (see figure), the crucible was an alumina cylinder and the mold material was plastic tie-down tape. In the second step, the patterned crucible is coated with metal. In one variation of the second step, a very thin layer containing or consisting of an electrically conductive material (e.g., gold, silver, or carbon) is painted or otherwise deposited on the mold-covered crucible, then the covering metal required for the specific application is electrodeposited on the very thin conducting layer. In another variation of the second step, the metal coat is formed by chemical vapor deposition. In the proof-of-concept demonstration, a layer of nickel 0.003 in. ( 0.08 mm) thick was electrodeposited. In the third step, the patterned material is removed. This is

  19. Insulin-like growth factor I induces proliferation and migration of porcine trophectoderm cells through multiple cell signaling pathways, including protooncogenic protein kinase 1 and mitogen-activated protein kinase.

    PubMed

    Jeong, Wooyoung; Song, Gwonhwa; Bazer, Fuller W; Kim, Jinyoung

    2014-03-25

    During early pregnancy, the developing conceptus is dependent upon a wide range of growth factors and nutrients that are secreted by or transported by uterine epithelia into the uterus at the maternal-conceptus interface for successful implantation and placentation. Among these factors, insulin-like growth factor-I (IGF-I) is known to play an important role in development of the early embryo and uterine endometrium. However, few studies have been conducted with pigs to determine IGF-I-induced functional effects on peri-implantation embryos such as activation of cell signaling cascades responsible for growth, proliferation and differentiation of cells of the conceptus. Therefore, the aim of this study was to analyze mRNA expression of endometrial IGF-I and its receptor, to examine the functional role of IGF-I on primary porcine trophectoderm (pTr) cells and to assess potential signaling pathways responsible for biological activities of IGF-1. In the present study, expression of endometrial type I IGF receptor (IGF-IR) mRNA increased significantly from Day 10 to Day 12 of pregnancy and the increase was greater for pregnant than cyclic gilts. Both IGF-I and IGF-IR mRNAs were abundant in endometrial luminal-, glandular epithelia, and stratum compactum stroma on Day 12 of pregnancy. In addition, IGF-I significantly induced phosphorylation of AKT1, ERK1/2 and RPS6 in a time- and concentration-dependent manner in pTr cells. Immunofluorescence microscopy revealed that IGF-I treated pTr cells exhibited increased abundance of phosphorylated (p)-AKT1 and p-ERK1/2 MAPK proteins in the nucleus and cytoplasm, and p-RPS6 proteins in the cytosol as compared to non-treated pTr cells. In the presence of the ERK1/2 MAPK inhibitor (U0126), IGF-I-induced AKT1 phosphorylation was not affected, whereas the PI3K inhibitor (LY294002) decreased IGF-I-induced phosphorylation of ERK1/2 and AKT1 proteins, and both the PI3K-AKT1 and ERK1/2 MAPK pathways were blocked by LY294002. Furthermore

  20. 29 CFR 1918.31 - Hatch coverings.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 'tween-decks unless all hatch beams are in place under the hatch covers. (c) Missing, broken, or poorly... covers and hatch beams not of uniform size shall be placed only in the hatch, deck, and section in...

  1. 29 CFR 1918.31 - Hatch coverings.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 'tween-decks unless all hatch beams are in place under the hatch covers. (c) Missing, broken, or poorly... covers and hatch beams not of uniform size shall be placed only in the hatch, deck, and section in...

  2. PNRC: a proline-rich nuclear receptor coregulatory protein that modulates transcriptional activation of multiple nuclear receptors including orphan receptors SF1 (steroidogenic factor 1) and ERRalpha1 (estrogen related receptor alpha-1).

    PubMed

    Zhou, D; Quach, K M; Yang, C; Lee, S Y; Pohajdak, B; Chen, S

    2000-07-01

    PNRC (proline-rich nuclear receptor coregulatory protein) was identified using bovine SF1 (steroidogenic factor 1) as the bait in a yeast two-hybrid screening of a human mammary gland cDNA expression library. PNRC is unique in that it has a molecular mass of 35 kDa, significantly smaller than most of the coregulatory proteins reported so far, and it is proline-rich. PNRC's nuclear localization was demonstrated by immunofluorescence and Western blot analyses. In the yeast two-hybrid assays, PNRC interacted with the orphan receptors SF1 and ERRalpha1 in a ligand-independent manner. PNRC was also found to interact with the ligand-binding domains of all the nuclear receptors tested including estrogen receptor (ER), androgen receptor (AR), glucocorticoid receptor (GR), progesterone receptor (PR), thyroid hormone receptor (TR), retinoic acid receptor (RAR), and retinoid X receptor (RXR) in a ligand-dependent manner. Functional AF2 domain is required for nuclear receptors to bind to PNRC. Furthermore, in vitro glutathione-S-transferase pull-down assay was performed to demonstrate a direct contact between PNRC and nuclear receptors such as SF1. Coimmunoprecipitation experiment using Hela cells that express PNRC and ER was performed to confirm the interaction of PNRC and nuclear receptors in vivo in a ligand-dependent manner. PNRC was found to function as a coactivator to enhance the transcriptional activation mediated by SF1, ERR1 (estrogen related receptor alpha-1), PR, and TR. By examining a series of deletion mutants of PNRC using the yeast two-hybrid assay, a 23-amino acid (aa) sequence in the carboxy-terminal region, aa 278-300, was shown to be critical and sufficient for the interaction with nuclear receptors. This region is proline rich and contains a SH3-binding motif, S-D-P-P-S-P-S. Results from the mutagenesis study demonstrated that the two conserved proline (P) residues in this motif are crucial for PNRC to interact with the nuclear receptors. The exact 23

  3. Updating the 2001 National Land Cover Database land cover classification to 2006 by using Landsat imagery change detection methods

    USGS Publications Warehouse

    Xian, G.; Homer, C.; Fry, J.

    2009-01-01

    The recent release of the U.S. Geological Survey (USGS) National Land Cover Database (NLCD) 2001, which represents the nation's land cover status based on a nominal date of 2001, is widely used as a baseline for national land cover conditions. To enable the updating of this land cover information in a consistent and continuous manner, a prototype method was developed to update land cover by an individual Landsat path and row. This method updates NLCD 2001 to a nominal date of 2006 by using both Landsat imagery and data from NLCD 2001 as the baseline. Pairs of Landsat scenes in the same season in 2001 and 2006 were acquired according to satellite paths and rows and normalized to allow calculation of change vectors between the two dates. Conservative thresholds based on Anderson Level I land cover classes were used to segregate the change vectors and determine areas of change and no-change. Once change areas had been identified, land cover classifications at the full NLCD resolution for 2006 areas of change were completed by sampling from NLCD 2001 in unchanged areas. Methods were developed and tested across five Landsat path/row study sites that contain several metropolitan areas including Seattle, Washington; San Diego, California; Sioux Falls, South Dakota; Jackson, Mississippi; and Manchester, New Hampshire. Results from the five study areas show that the vast majority of land cover change was captured and updated with overall land cover classification accuracies of 78.32%, 87.5%, 88.57%, 78.36%, and 83.33% for these areas. The method optimizes mapping efficiency and has the potential to provide users a flexible method to generate updated land cover at national and regional scales by using NLCD 2001 as the baseline. ?? 2009 Elsevier Inc.

  4. Thirty years of land-cover change in Bolivia.

    PubMed

    Killeen, Timothy J; Calderon, Veronica; Soria, Liliana; Quezada, Belem; Steininger, Marc K; Harper, Grady; Solórzano, Luis A; Tucker, Compton J

    2007-11-01

    Land-cover change in eastern lowland Bolivia was documented using Landsat images from five epochs for all landscapes situated below the montane tree line at approximately 3000 m, including humid forest, inundated forest, seasonally dry forest, and cloud forest, as well as scrublands and grasslands. Deforestation in eastern Bolivia in 2004 covered 45,411 km2, representing approximately 9% of the original forest cover, with an additional conversion of 9042 km2 of scrub and savanna habitats representing 17% of total historical land-cover change. Annual rates of land-cover change increased from approximately 400 km2 y(-1) in the 1960s to approximately 2900 km2 y(-1) in the last epoch spanning 2001 to 2004. This study provides Bolivia with a spatially explicit information resource to monitor future land-cover change, a prerequisite for proposed mechanisms to compensate countries for reducing carbon emissions as a result of deforestation. A comparison of the most recent epoch with previous periods shows that policies enacted in the late 1990s to promote forest conservation had no observable impact on reducing deforestation and that deforestation actually increased in some protected areas. The rate of land-cover change continues to increase linearly nationwide, but is growing faster in the Santa Cruz department because of the expansion of mechanized agriculture and cattle farms.

  5. Field Water Balance of Landfill Final Covers

    EPA Science Inventory

    Landfill covers are critical to waste containment, yet field performance of specific cover designs has not been well documented and seldom been compared in side-by-side testing. A study was conducted to assess the ability of landfill final covers to control percolation into unde...

  6. 36 CFR 312.1 - Areas covered.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Areas covered. 312.1 Section 312.1 Parks, Forests, and Public Property CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY PROHIBITION OF DISCRIMINATORY PRACTICES IN WATER RESOURCE DEVELOPMENT PROJECTS § 312.1 Areas covered. The regulation covered...

  7. 36 CFR 312.1 - Areas covered.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Areas covered. 312.1 Section 312.1 Parks, Forests, and Public Property CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY PROHIBITION OF DISCRIMINATORY PRACTICES IN WATER RESOURCE DEVELOPMENT PROJECTS § 312.1 Areas covered. The regulation covered...

  8. 36 CFR 312.1 - Areas covered.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Areas covered. 312.1 Section 312.1 Parks, Forests, and Public Property CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY PROHIBITION OF DISCRIMINATORY PRACTICES IN WATER RESOURCE DEVELOPMENT PROJECTS § 312.1 Areas covered. The regulation covered...

  9. 36 CFR 312.1 - Areas covered.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Areas covered. 312.1 Section 312.1 Parks, Forests, and Public Property CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY PROHIBITION OF DISCRIMINATORY PRACTICES IN WATER RESOURCE DEVELOPMENT PROJECTS § 312.1 Areas covered. The regulation covered...

  10. 36 CFR 312.1 - Areas covered.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Areas covered. 312.1 Section 312.1 Parks, Forests, and Public Property CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY PROHIBITION OF DISCRIMINATORY PRACTICES IN WATER RESOURCE DEVELOPMENT PROJECTS § 312.1 Areas covered. The regulation covered...

  11. Managing cover crops: an economic perspective

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Common reasons given by producers as to why they do not adopt cover crops are related to economics: time, labor, and cost required for planting and managing cover crops. While many of the agronomic benefits of cover crops directly relate to economics, there are costs associated with adopting the pra...

  12. 49 CFR 193.2167 - Covered systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Covered systems. 193.2167 Section 193.2167...: FEDERAL SAFETY STANDARDS Design Impoundment Design and Capacity § 193.2167 Covered systems. A covered impounding system is prohibited except for concrete wall designed tanks where the concrete wall is an...

  13. 21 CFR 880.6185 - Cast cover.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Cast cover. 880.6185 Section 880.6185 Food and....6185 Cast cover. (a) Identification. A cast cover is a device intended for medical purposes that is made of waterproof material and placed over a cast to protect it from getting wet during a shower or...

  14. 21 CFR 880.6185 - Cast cover.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Cast cover. 880.6185 Section 880.6185 Food and....6185 Cast cover. (a) Identification. A cast cover is a device intended for medical purposes that is made of waterproof material and placed over a cast to protect it from getting wet during a shower or...

  15. 21 CFR 880.6185 - Cast cover.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Cast cover. 880.6185 Section 880.6185 Food and....6185 Cast cover. (a) Identification. A cast cover is a device intended for medical purposes that is made of waterproof material and placed over a cast to protect it from getting wet during a shower or...

  16. Selection of fungi by candidate cover crops

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Diversified cropping systems that incorporate year-round ground cover, are known to maintain healthy soils. Information is available for producers regarding the benefits of specific cover crop species for soil fertility, weed and pest management. Even though it is widely recognized that cover crops ...

  17. 49 CFR 192.327 - Cover.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Lines and Mains § 192.327 Cover. (a) Except as provided in paragraphs (c), (e), (f), and (g) of this section, each buried transmission line must be installed with a minimum cover as follows: Location Normal... of a transmission line or main with the minimum cover, the transmission line or main may be...

  18. 49 CFR 192.327 - Cover.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Lines and Mains § 192.327 Cover. (a) Except as provided in paragraphs (c), (e), (f), and (g) of this section, each buried transmission line must be installed with a minimum cover as follows: Location Normal... of a transmission line or main with the minimum cover, the transmission line or main may be...

  19. Completion of the National Land Cover Database (NLCD) 1992-2001 Land Cover Change Retrofit Product

    EPA Science Inventory

    The Multi-Resolution Land Characteristics Consortium has supported the development of two national digital land cover products: the National Land Cover Dataset (NLCD) 1992 and National Land Cover Database (NLCD) 2001. Substantial differences in imagery, legends, and methods betwe...

  20. Cloud Cover and Wildfire Variations in Vietnam and Southeast Asia

    NASA Astrophysics Data System (ADS)

    Lasko, K.

    2015-12-01

    Monitoring fires from space is constrained by cloud cover, particularly in tropical regions. Cloud cover-fire variations were assessed using the CERES SSF1Deg and MODIS active fire (MCD14ML) products in Vietnam and Southeast Asia. Twelve years (2003-2014) of data have been analyzed spatially and temporally at four spatial scales: a) country level; b). 1x1 degree scale; c). land cover type; d). regions. Country-level results suggested Vietnam having the highest monthly cloud cover (72.37%) followed by Cambodia (69.69%), Laos (67.64%), Thailand (67.58%), and Myanmar (59.90%). Strong negative correlation between cloud cover and MODIS active fires has been observed during the biomass burning months (Jan-Apr). Of the different countries, Vietnam also had the lowest monthly fire detections. Pixel by pixel spatial correlation at 1x1 degree suggested negative fire-cloud relationships over the Red River Delta of Vietnam, the forests of northern Laos, and agriculture-dominated peninsulas of Thailand and Myanmar. Among the different land cover types, the average monthly cloud cover varied between 64% - 66%. Further, results from daily data showed the Red River Delta to have consistently more cloud cover (20-40% more) than the Mekong River Delta in Vietnam, with fewer fire detections in the former than the latter. The study highlights potential fire under-detection due to clouds. Our results highlight spatial and temporal variations in cloud-fire relationships and the difficulty of using optical data for fire detection and characterization in persistently cloudy regions.

  1. CASA Forest Cover Change Data Sets

    NASA Technical Reports Server (NTRS)

    Potter, Christopher S.

    2012-01-01

    Deforestation and forest fires are global land cover changes that can be caused by both natural and human factors. Although monitoring forest fires in near-real time is critical for operational wildfire management, mapping historical wildfires in a spatially explicit fashion is also important for a number of reasons, including climate change studies (e.g., examining the relationship between rising temperatures and frequency of fires), fuel load management (e.g., deciding when and where to conduct controlled burns), and carbon cycle studies (e.g., quantifying how much CO2 is emitted by fires and for emissions reduction efforts under the United Nations programs for Reducing Emissions from Deforestation and Degradation -- REDD).

  2. Design of top covers supporting aerobic in situ stabilization of old landfills - An experimental simulation in lysimeters

    SciTech Connect

    Hrad, Marlies; Huber-Humer, Marion; Wimmer, Bernhard; Reichenauer, Thomas G.

    2012-12-15

    Highlights: Black-Right-Pointing-Pointer Tested engineered covers as surrogate to gas extraction during and after in situ aeration. Black-Right-Pointing-Pointer Examined how covers influence gas emissions, water balance and leachate generation. Black-Right-Pointing-Pointer Investigated effect of top covers on air-distribution in waste mass during aeration. Black-Right-Pointing-Pointer We suggest criteria and cover design to meet the demands during and after aeration. Black-Right-Pointing-Pointer Such cover systems may offer greenhouse gas emission reduction also after active aeration. - Abstract: Landfill aeration by means of low pressure air injection is a promising tool to reduce long term emissions from organic waste fractions through accelerated biological stabilization. Top covers that enhance methane oxidation could provide a simple and economic way to mitigate residual greenhouse gas emissions from in situ aerated landfills, and may replace off-gas extraction and treatment, particularly at smaller and older sites. In this respect the installation of a landfill cover system adjusted to the forced-aerated landfill body is of great significance. Investigations into large scale lysimeters (2 Multiplication-Sign 2 Multiplication-Sign 3 m) under field conditions have been carried out using different top covers including compost materials and natural soils as a surrogate to gas extraction during active low pressure aeration. In the present study, the emission behaviour as well as the water balance performance of the lysimeters has been investigated, both prior to and during the first months of in situ aeration. Results reveal that mature sewage sludge compost (SSC) placed in one lysimeter exhibits in principle optimal ambient conditions for methanotrophic bacteria to enhance methane oxidation. Under laboratory conditions the mature compost mitigated CH{sub 4} loadings up to 300 l CH{sub 4}/m{sup 2} d. In addition, the compost material provided high air permeability

  3. Comparison of Passive and Active Remotely Sensed Microwave Soil Moisture Retrievals using Soil Moisture Simulations (GLDAS) over Different Land Covers in East Asia: using SMOS, ASCAT, AMSR2, and FY-3B

    NASA Astrophysics Data System (ADS)

    Kim, H.; Choi, M.

    2015-12-01

    Soil moisture is a key variable in environmental systems since water and energy fluxes at the surface and atmosphere interface are strongly dependent on soil moisture. Furthermore, soil moisture has been identified as one of the "Essential Climate Variables" expected to improve climate predictions and near-future forecasting. Several studies have been conducted to acquire soil moisture estimates from spaceborne microwave instruments. As a results, soil moisture data is now globally available using several kinds of satellites with different temporal or spatial resolutions. In this study, we investigate four satellite-based soil moisture products, Soil Moisture and Ocean Salinity (SMOS), Advanced Scatterometer (ASCAT), Advanced Microwave Scanning Radiometer-2 (AMSR2), and Fengyun-3B (FY-3B), compared to an independent reference, Global Land Data Assimilation System (GLDAS) soil moisture datasets over East Asia. Biosphere Atmosphere Transfer Scheme (BATS) dataset was utilized for land cover classification. The relationship between the GLDAS soil moisture and satellite products was analyzed by using of temporal correlation, unbiased root mean square difference, mean bias, and lagged variables. Especially, over the arid regions (deserts and semi deserts), SMOS showed the best consistency with GLDAS and it was found that ASCAT soil moisture exhibit best correlation versus GLDAS except desert and semi desert regions (Figure 1.). In addition, performances of AMSR2 soil moisture products based on Land Parameter Retrieval Model (LPRM) and FY-3B over East Asia were also very encouraging (the period 2013).

  4. The boiling crisis phenomenon on capillary-porous covering

    SciTech Connect

    Afanasiev, B.A.; Smirnov, G.F.; Poniewski, M.E.; Wojcik, T.M.

    1995-12-31

    Physical foundations and mathematical model of the boiling crisis phenomenon on capillary-porous covering -- based on modified Kutateladze-Zuber hydrodynamic hypothesis -- have been formulated. The modification has included new factors in boiling mechanism on capillary-porous covering, such as energy losses due to liquid and vapor transportation through pores, droplet ejection, and capillary potential. The theoretical model was verified against experimental data for water and ethanol, and for metallic screen coverings featuring varying parameters, such as porosity, thickness of the porous layer, pore cell diameter, and pressure. A semi-empirical formula that was drawn up from the model matches the experimental data quite well. It will allow one to determine the optimum porous structure to transfer maximum heat flux in heat pipes and other types of boiling heat transfer devices with porous coverings.

  5. Coordination and standardization of federal sedimentation activities

    USGS Publications Warehouse

    Glysson, G. Douglas; Gray, John R.

    1997-01-01

    - precipitation information critical to water resources management. Memorandum M-92-01 covers primarily freshwater bodies and includes activities, such as "development and distribution of consensus standards, field-data collection and laboratory analytical methods, data processing and interpretation, data-base management, quality control and quality assurance, and water- resources appraisals, assessments, and investigations." Research activities are not included.

  6. Enhanced snow cover products from MODIS for the hydrologic sciences

    NASA Astrophysics Data System (ADS)

    Painter, T. H.; Bales, R.

    2004-12-01

    Near mountain ranges of the globe, over a billion people use melt of the seasonal snow cover as the dominant source of their water resources. These regions are increasingly experiencing the pressing, coupled implications of climate change, drought, and population/demand increase. Enhanced snow cover products have been developed using a multiple endmember spectral mixture analysis model that inverts MODIS surface reflectance products (MOD09) for fractional snow cover, plus the grain size and albedo of the fractional snow cover. Referred to as the MODIS Snow Covered Area and Grain Size/Albedo (MODSCAG) model, this tool is specifically aimed at providing an accurate estimate of snowcover for regional studies in mountainous areas across the globe. The model uses spectral libraries generated with a radiative transfer model for varying grain size snow, adapting the spectral library according to the specific scene solar geometry. Both the albedo and snow-covered area products stimulate advances in hydrologic forecasting by providing more accurate, spatially distributed data than are currently available for assimilation and model evaluation. Data are being developed and provided through an end-to-end NASA REASoN project that includes: (i) standard and custom product development, (ii) distribution through multiple user interfaces and (iii) user support for product evaluation and applications. Currently, MODSCAG is producing snow cover products for hydrologic research clients working in the Colorado River Basin, the Sierra Nevada of California, the Columbia River Basin, and central Tibet. Within the framework of the REASoN project, MODSCAG is designed to address `client' research needs in snow-covered basins around the globe. In this work, we present the introduction of the MODSCAG fractional snow cover products into a model of basin hydrology in the Sierra Nevada. We analyze the differences between the MODSCAG fractional product and the standard MODIS binary snowcover

  7. Embellished String Prints. Cover Story.

    ERIC Educational Resources Information Center

    Smith, Mary Ruth

    1999-01-01

    Focuses on a printmaking activity in which students create embellished string prints using the relief process of string glued to chip board. Explains that string prints can easily be embellished with oil pastels. Provides a description of the procedure and a list of materials and methods. (CMK)

  8. Scientific Analysis Cover Sheet for Radionuclide Screening

    SciTech Connect

    G. Ragan

    2002-08-09

    The waste forms under consideration for disposal in the proposed repository at Yucca Mountain contain scores of radionuclides (Attachments V and VI). It would be impractical and highly inefficient to model all of these radionuclides in a total system performance assessment (TSPA). Thus, the purpose of this radionuclide screening analysis is to remove from further consideration (screen out) radionuclides that are unlikely to significantly contribute to radiation dose to the public from the proposed nuclear waste repository at Yucca Mountain. The remaining nuclides (those screened in) are recommended for consideration in TSPA modeling for license application. This analysis also covers radionuclides that are not screened in based on dose, but need to be included in TSPA modeling for other reasons. For example, U.S. Environmental Protection Agency (EPA) and U.S. Nuclear Regulatory Commission (NRC) regulations require consideration of the combined activity of Ra-226 and Ra-228 in groundwater (40 CFR 197.30, 10 CFR 63.331). Also, Cm-245, Pu-241, and U-235 decay indirectly to potentially important radionuclides, and are not identified by the screening analysis as important. The radionuclide screening analysis separately considers two different postclosure time periods: the 10,000-y regulatory period for the proposed repository at Yucca Mountain and the period after 10,000 y up to 1 million y after emplacement. The incremental effect of extending the screening for the regulatory period to 20,000 y is also addressed. Four release scenarios are considered: (1) the nominal scenario, which entails long-term degradation of disposal containers and waste forms, (2) a human-intrusion scenario, (3) an intrusive igneous event, and (4) an eruptive igneous event. Because the first three scenarios require groundwater transport, they are called groundwater scenarios below. The screening analysis considers the following waste forms: spent boiling water reactor (BWR) fuel, spent

  9. Land Application of Wastes: An Educational Program. Role of Vegetative Cover - Module 7, Objectives, and Script.

    ERIC Educational Resources Information Center

    Clarkson, W. W.; And Others

    This module discusses some of the objectives of incorporating vegetative cover in land treatment systems. Specific crops and forest cover are mentioned in relation to benefits associated with each, and specific treatment alternatives (irrigation, overland flow, and rapid infiltration) are included in relation to vegetative cover considerations.…

  10. 42 CFR 403.904 - Reports of payments or other transfers of value to covered recipients.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... group setting where the cost of each individual covered recipient's meal is not separately identifiable... individuals who partook in the meal (including both covered recipients and non-covered recipients, such as office staff). The per person value of the meal must be reported as a payment or other transfer of...

  11. Internal combustion engine timing chain cover

    SciTech Connect

    Carvalho, A.

    1991-03-12

    This patent describes an internal combustion engine end and timing chain cover for a multi-cylinder, automotive vehicle type engine having a generally horizontally arranged engine block with a forward end and upper and lower portions, and a generally horizontally extending crankshaft with a forward end portion, and a timing chain mechanism on the block forward end above crankshaft: It comprises: a unitary, a cast metal, dish-like, cover having a forward wall surrounded by an integral edge wall shaped to fit over and mount upon the forward end of the engine block with the cover forming an enclosed cavity at the front end of the engine and with the cover cavity adapted to receive and enclose the engine timing chain mechanism portions located on the engine forward end; the cover having a hole formed in its lower portion in alignment with the engine crankshaft, and with the engine crankshaft forward end portion extending through the hole and being surrounded by a seal; the seal being mounted within the hole in the cover, within an open, rabbit-like groove surrounding the hold and opening forwardly of the cover, so that the seal may be removed in a forward direction relative to the engine and replaced without removing the cover when the cover is mounted upon the block with the crankshaft extending therethrough; and bolt receiving openings formed on the cover edge wall through which mounting bolts may be positioned for fastening the cover upon the engine.

  12. Factors affecting the thermal environment of Agassiz’s Desert Tortoise (Gopherus agassizii) cover sites in the Central Mojave Desert during periods of temperature extremes

    USGS Publications Warehouse

    Mack, Jeremy S.; Berry, Kristin H.; Miller, David; Carlson, Andrea S.

    2015-01-01

    Agassiz's Desert Tortoises (Gopherus agassizii) spend >95% of their lives underground in cover sites that serve as thermal buffers from temperatures, which can fluctuate >40°C on a daily and seasonal basis. We monitored temperatures at 30 active tortoise cover sites within the Soda Mountains, San Bernardino County, California, from February 2004 to September 2006. Cover sites varied in type and structural characteristics, including opening height and width, soil cover depth over the opening, aspect, tunnel length, and surficial geology. We focused our analyses on periods of extreme temperature: in summer, between July 1 and September 1, and winter, between November 1 and February 15. With the use of multivariate regression tree analyses, we found cover-site temperatures were influenced largely by tunnel length and subsequently opening width and soil cover. Linear regression models further showed that increasing tunnel length increased temperature stability and dampened seasonal temperature extremes. Climate change models predict increased warming for southwestern North America. Cover sites that buffer temperature extremes and fluctuations will become increasingly important for survival of tortoises. In planning future translocation projects and conservation efforts, decision makers should consider habitats with terrain and underlying substrate that sustain cover sites with long tunnels and expanded openings for tortoises living under temperature extremes similar to those described here or as projected in the future.

  13. [Covering of a thoraco-lumbar defect by omentoplasty].

    PubMed

    Le Fourn, B; Loirat, Y; Sartre, J Y; Lejeune, F; Pannier, M

    1997-02-01

    With a case of thoraco-lumbar defect, the authors discuss about different procedures to cover it. In this place, the better procedure is certainly the latissimus dorsi flap, in all combinations. The indication for omentoplasty at this spinal site should not be performed by first intention but by exclusion of other procedures, as in the case considered by the authors. It was a 37-year-old man, paraplegic from the age of 16, with a deep chronic spinal wound, secondary to sepsis of a posterior segmental fixations. A staphylococcus aureus infection which developed as a surgical complication was initially treated with antibiotics and surgical cleaning procedures without removing instrumentation. However, the infection remained active and the material was finally removed. Spinal immobilisation was strengthened by external fixation. The area was cleared of all suspect material, including bone graft, leaving a wide back-wound open to the spine. Spontaneous healing was first attempted, but the size and the chronicity of the wound led us to use pedicled greater omentum to close the defect. The omentum was pedicled on the right gastroepiploic vessels and transferred to the back wound through the posterior abdominal wall muscles, next to the right kidney. This procedure allows rapid healing. In association with suitable antibiotics, it has prevented any recurrent infection after 18 months of follow-up. It was no feasible to cover the wound with a latissimus dorsi flap, considering the importance of this muscle in the movements of a paraplegic and considering the initial impossibility of removing the external fixation.

  14. Cover preference of the Carolina madtom (Noturus furiosus), an imperiled, endemic southeastern stream fish

    USGS Publications Warehouse

    Midway, S.R.; Aday, D.D.; Kwak, T.J.; Gross, K.

    2010-01-01

    In a laboratory setting, we investigated cover preference of the Carolina madtom (Noturus furiosus), an imperiled, endemic southeastern USA stream fish. Fish were tested individually and given 24 hours to make a selection from four cover options, including rock, leaf pack, mussel shell, and an artificial cover unit. Among 30 trials, Carolina madtom preferred the artificial cover unit, selecting it 63% of the time. Rock was selected 23% of the time, and leaf pack 13%. Mussel shells were not selected during any trial.

  15. Cover preference of the Carolina madtom (Noturus furiosus), an imperiled, indemic southeastern stream fish

    USGS Publications Warehouse

    Midway, S.R.; Aday, D.D.; Kwak, Thomas J.; Gross, K.

    2010-01-01

    In a laboratory setting, we investigated cover preference of the Carolina madtom (Noturus furiosus), an imperiled, endemic southeastern USA stream fish. Fish were tested individually and given 24 hours to make a selection from four cover options, including rock, leaf pack, mussel shell, and an artificial cover unit. Among 30 trials, Carolina madtom preferred the artificial cover unit, selecting it 63% of the time. Rock was selected 23% of the time, and leaf pack 13%. Mussel shells were not selected during any trial.

  16. Including Conflict in Creative Writing.

    ERIC Educational Resources Information Center

    Litvin, Martin

    Conflict is the basis of all stories and thus should appear in some form in the first sentence. There are three kinds of conflict: people vs. people; people vs. nature; and people vs. themselves. Conflict must be repeated in all the various elements of the story's structure, including the plot, which is the plan of action telling what happens to…

  17. Family Living, Including Sex Education.

    ERIC Educational Resources Information Center

    Forlano, George

    This volume describes and evaluates 21 selected New York City Board of Education Umbrella Programs for the 1974-1975 school year. The programs include: (1) the parent resource center, (2) the teacher self-help program, (3) the East Harlem pre-kindergarten center, (4) the Brooklyn College volunteer tutoring program, (5) the parent education for…

  18. Development of multi-year land cover data to assess wildfire impacts to coastal watersheds and the nearshore environment

    NASA Astrophysics Data System (ADS)

    Morrison, Katherine D.

    In the Mediterranean ecosystems of coastal California, wildfire is a common disturbance that can significantly alter vegetation in watersheds that transport sediment and nutrients to the adjacent nearshore oceanic environment. We assess the impact of two wildfires that burned in 2008 on land cover and to the nearshore environment along the Big Sur coast in central California. We created a multi-year land cover dataset to assess changes to coastal watersheds as a result of fire. This land cover dataset was then used to model changes in nonpoint source pollutants transported to the nearshore environment. Results indicate post-fire increases in percent export compared to pre-fire years and also link wildfire severity to the specific land cover changes that subsequently increase exports of pollutants and sediment to the nearshore environment. This approach is a replicable across watersheds and also provides a framework for including the nearshore environment as a value at risk terrestrial land management revolving around wildfire, including suppression, thinning, and other activities that change land cover at a landscape scale.

  19. Update of the BIPM comparison BIPM.RI(II)-K1.F-18 of activity measurements of the radionuclide 18F to include the 2010 result of the LNE-LNHB (France)

    NASA Astrophysics Data System (ADS)

    Michotte, C.; Ratel, G.; Courte, S.; Cassette, P.; Moune, M.

    2016-01-01

    Since 2001, six national metrology institutes (NMI) have submitted seven samples of known activity of 18F to the International Reference System (SIR) for activity comparison at the Bureau International des Poids et Mesures (BIPM), with comparison identifier BIPM.RI(II)-K1.F-18. The values of the activity submitted were between about 1 MBq and 18 MBq. The primary standardization result for the LNE-LNHB, France, replaces their earlier result of 2002 and the key comparison reference value (KCRV) has been recalculated. In the frame of the BIPM.RI(II)-K4.F18 comparison, the NPL updated their result in the KCDB. Consequently there are now five results in the BIPM.RI(II)-K1.F-18 comparison. The degrees of equivalence between each equivalent activity measured in the SIR and the updated KCRV have been calculated and the results are given in the form of a table. A graphical presentation is also given. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCRI, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  20. System for reducing heat losses from indoor swimming pools by use of automatic covers. Report No. 3

    SciTech Connect

    Not Available

    1994-09-30

    This progress report covers the period July 1, 1994 through September 30, 1994, and summarizes continuing work on developing deloyable covers for indoor swimming pools. This work includes design and development of motor controllers to deploy and roll up pool covers, reels, cover material of polyethylene and foam filled laminates, and plans for field deployment of a system, where energy savings can be monitored.

  1. 24 CFR 3.600 - Notice of covered programs.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Notice of covered programs. 3.600 Section 3.600 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  2. 24 CFR 3.600 - Notice of covered programs.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Notice of covered programs. 3.600 Section 3.600 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  3. 24 CFR 3.600 - Notice of covered programs.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Notice of covered programs. 3.600 Section 3.600 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  4. 24 CFR 3.600 - Notice of covered programs.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Notice of covered programs. 3.600 Section 3.600 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  5. 24 CFR 3.600 - Notice of covered programs.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Notice of covered programs. 3.600 Section 3.600 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING...

  6. 14 CFR § 1253.600 - Notice of covered programs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Notice of covered programs. § 1253.600 Section § 1253.600 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL...

  7. 14 CFR 1253.600 - Notice of covered programs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Notice of covered programs. 1253.600 Section 1253.600 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE...

  8. 14 CFR 1253.600 - Notice of covered programs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Notice of covered programs. 1253.600 Section 1253.600 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE...

  9. 14 CFR 1253.600 - Notice of covered programs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Notice of covered programs. 1253.600 Section 1253.600 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE...

  10. 14 CFR 1253.600 - Notice of covered programs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Notice of covered programs. 1253.600 Section 1253.600 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE...

  11. Further investigation of the spontaneous and evoked activity of the primary neurons of statoreceptors (and other receptors) of the labyrinth of the bullfrog before, during and after an extended period of weightlessness, including alternative intervals of artificial gravity

    NASA Technical Reports Server (NTRS)

    1977-01-01

    Vestibular neuron activity was examined by studying nerve stimulation and evoked response. A cooling element, applied to the nerve consisted of a silver hook through which a coolant fluid flowed. Temperature changes were recorded via microtermistors on an eight channel brush recorder, together with response. Diffusion of the cooling effect was measured, recovery time was assessed, and the nerve was then studied hystologically and ultrastructurally. Problems in frog preparation were discussed along with problems in maintaining healthy specimens and bacteria controlled aquaria.

  12. Development of a resettable, flexible aperture cover

    NASA Technical Reports Server (NTRS)

    Christiansen, Scott

    1992-01-01

    A flexible aperture cover and latch were developed for the Thermal Ion Detection Experiment (TIDE). The latch utilized a high-output paraffin (HOP) linear motor to supply the force to operate the latch. The initial approach for the cover was to use a heat-treated, coiled strip of 0.05 mm (.002-inch)-thick beryllium-copper as the cover. Development test results showed that one end of the cover developed a trajectory during release that threatened to impact against adjacent instruments. An alternative design utilizing constant force springs and a flexible, metallized Kapton cover was then tested. Results from development tests, microgravity tests, and lessons learned during the development of the aperture cover are discussed.

  13. A new porous polyetherurethane wound covering.

    PubMed

    Bruin, P; Jonkman, M F; Meijer, H J; Pennings, A J

    1990-02-01

    A polyetherurethane (PEU) wound covering with non-interconnected micropores up to approximately 5 microns has been prepared by means of a phase inversion process. This highly elastic, very thin (15-20 microns), pliable wound covering showed good, immediate adherence to wet wound surfaces and high water vapor permeability, but was impermeable to bacteria. In guinea pigs epidermal wound healing of partial-thickness wounds under PEU wound coverings was accelerated compared with uncovered controls and an occlusive wound covering, OpSite. Water in liquid form or wound exudate could not leak through the PEU covering, but its high water vapor permeability induced concentration of the wound exudate into a jellylike clot layer, which apparently accelerated reepithelialization. The main conclusion from a clinical study on 20 donor sites was that the use of the PEU covering reduced pain, besides prevention of fluid retention. No differences in epithelialization were seen in comparison to tulle gras-treated wounds.

  14. Lightweight composite fighting cover prototype development program

    SciTech Connect

    Wrenn, G.E. Jr.; Frame, B.J.; Gwaltney, R.C.; Akerman, M.A.

    1996-07-01

    The U.S. Army Field Assistance Science and Technology Program requested Oak Ridge National Laboratory (ORNL) to demonstrate the use of lightweight composite materials in construction of overhead covers for reinforced infantry fighting positions. In recent years, ORNL researchers have designed and tested several concepts for lightweight ballistic protection structures, and they have developed numerous prototype composite structures for military and civilian applications. In the current program, composite panel designs and materials are tested and optimized to meet anticipated static and dynamic load conditions for the overhead cover structure. Ten prototype composite covers were built at ORNL for use in Army field tests. Each composite cover has a nominal surface area of 12 ft[sup 2] and a nominal weight of 8 lb. Four of the prototypes are made with folding sections to improve their handling characteristics. The composite covers exhibit equivalent performance in Army field tests to covers made with conventional materials that weigh four times as much.

  15. Track and Field Guide including Cross Country, Pentathlon Scoring Tables and Rules for Intercollegiate Meets and Championships with Official Rules. Janauary 1974-January 1976.

    ERIC Educational Resources Information Center

    Thompson, Donnis H., Ed.

    This guide includes information on cross country running, pentathlon scoring tables, and rules for intercollegiate meets and championships, following an introductory portion on the organization's credo and standards. The first section covers track activities for children, coaching techniques, the benefits of weight training, and some practical…

  16. Including Students with Visual Impairments: Softball

    ERIC Educational Resources Information Center

    Brian, Ali; Haegele, Justin A.

    2014-01-01

    Research has shown that while students with visual impairments are likely to be included in general physical education programs, they may not be as active as their typically developing peers. This article provides ideas for equipment modifications and game-like progressions for one popular physical education unit, softball. The purpose of these…

  17. An Integrated Biochemistry Laboratory, Including Molecular Modeling

    NASA Astrophysics Data System (ADS)

    Hall, Adele J. Wolfson Mona L.; Branham, Thomas R.

    1996-11-01

    The dilemma of designing an advanced undergraduate laboratory lies in the desire to teach and reinforce basic principles and techniques while at the same time exposing students to the excitement of research. We report here on a one-semester, project-based biochemistry laboratory that combines the best features of a cookbook approach (high success rate, achievement of defined goals) with those of an investigative, discovery-based approach (student involvement in the experimental design, excitement of real research). Individual modules may be selected and combined to meet the needs of different courses and different institutions. The central theme of this lab is protein purification and design. This laboratory accompanies the first semester of biochemistry (Structure and Function of Macromolecules, a course taken mainly by junior and senior chemistry and biological chemistry majors). The protein chosen as the object of study is the enzyme lysozyme, which is utilized in all projects. It is suitable for a student lab because it is easily and inexpensively obtained from egg white and is extremely stable, and its high isoelectric point (pI = 11) allows for efficient separation from other proteins by ion-exchange chromatography. Furthermore, a literature search conducted by the resourceful student reveals a wealth of information, since lysozyme has been the subject of numerous studies. It was the first enzyme whose structure was determined by crystallography (1). Hendrickson et al. (2) have previously described an intensive one-month laboratory course centered around lysozyme, although their emphasis is on protein stability rather than purification and engineering. Lysozyme continues to be the focus of much exciting new work on protein folding and dynamics, structure and activity (3 - 5). This lab course includes the following features: (i) reinforcement of basic techniques, such as preparation of buffers, simple enzyme kinetics, and absorption spectroscopy; (ii

  18. Utilizing Multiple Datasets for Snow Cover Mapping

    NASA Technical Reports Server (NTRS)

    Tait, Andrew B.; Hall, Dorothy K.; Foster, James L.; Armstrong, Richard L.

    1999-01-01

    Snow-cover maps generated from surface data are based on direct measurements, however they are prone to interpolation errors where climate stations are sparsely distributed. Snow cover is clearly discernable using satellite-attained optical data because of the high albedo of snow, yet the surface is often obscured by cloud cover. Passive microwave (PM) data is unaffected by clouds, however, the snow-cover signature is significantly affected by melting snow and the microwaves may be transparent to thin snow (less than 3cm). Both optical and microwave sensors have problems discerning snow beneath forest canopies. This paper describes a method that combines ground and satellite data to produce a Multiple-Dataset Snow-Cover Product (MDSCP). Comparisons with current snow-cover products show that the MDSCP draws together the advantages of each of its component products while minimizing their potential errors. Improved estimates of the snow-covered area are derived through the addition of two snow-cover classes ("thin or patchy" and "high elevation" snow cover) and from the analysis of the climate station data within each class. The compatibility of this method for use with Moderate Resolution Imaging Spectroradiometer (MODIS) data, which will be available in 2000, is also discussed. With the assimilation of these data, the resolution of the MDSCP would be improved both spatially and temporally and the analysis would become completely automated.

  19. The Cover Time of Cartesian Product Graphs

    NASA Astrophysics Data System (ADS)

    Abdullah, Mohammed; Cooper, Colin; Radzik, Tomasz

    Let P = G□H be the cartesian product of graphs G,H. We relate the cover time COV[P] of P to the cover times of its factors. When one of the factors is in some sense larger than the other, its cover time dominates, and can become of the same order as the cover time of the product as a whole. Our main theorem effectively gives conditions for when this holds. The probabilistic technique which we introduce, based on the blanket time, is more general and may be of independent interest, as might some of our lemmas.

  20. Heat resistant protective hand covering

    NASA Technical Reports Server (NTRS)

    Sidman, K. R.; Arons, I. J. (Inventor)

    1984-01-01

    The heat resistant, protective glove is made up of first and second shell sections which define a palm side and a backside, respectively. The first shell section is made of a twill wave fabric of a temperature-resistant aromatic polyamide fiber. The second shell section is made of a knitted fabric of a temperature-resistant aromatic polyamide fiber. The first and second shell sections are secured to one another, e.g., by sewing, to provide the desired glove configuration and an opening for insertion of the wearer's hand. The protective glove also includes a first liner section which is secured to and overlies the inner surface of the first shell section and is made of a felt fabric of a temperature-resistant aromatic polyamide fiber and has a flame resistant, elastomenic coating on the surface facing and overlying the inner surface of the first shell section.

  1. Genome-wide identification of Drosophila Hb9 targets reveals a pivotal role in directing the transcriptome within eight neuronal lineages, including activation of Nitric Oxide Synthase and Fd59a/Fox-D

    PubMed Central

    Lacin, Haluk; Rusch, Jannette; Yeh, Raymond T.; Fujioka, Miki; Wilson, Beth A.; Zhu, Yi; Robie, Alice A.; Mistry, Hemlata; Wang, Ting; Jaynes, James B.; Skeath, James B.

    2014-01-01

    Hb9 is a homeodomain-containing transcription factor that acts in combination with Nkx6, Lim3, and Tail-up (Islet) to guide the stereotyped differentiation, connectivity, and function of a subset of neurons in Drosophila. The role of Hb9 in directing neuronal differentiation is well documented, but the lineage of Hb9+ neurons is only partly characterized, its regulation is poorly understood, and most of the downstream genes through which it acts remain at large. Here, we complete the lineage tracing of all embryonic Hb9+ neurons (to eight neuronal lineages) and provide evidence that hb9, lim3, and tail-up are coordinately regulated by a common set of upstream factors. Through the parallel use of micro-array gene expression profiling and the Dam-ID method, we searched for Hb9-regulated genes, uncovering transcription factors as the most over-represented class of genes regulated by Hb9 (and Nkx6) in the CNS. By a nearly ten-to-one ratio, Hb9 represses rather than activates transcription factors, highlighting transcriptional repression of other transcription factors as a core mechanism by which Hb9 governs neuronal determination. From the small set of genes activated by Hb9, we characterized the expression and function of two – fd59a/foxd, which encodes a transcription factor, and Nitric oxide synthase. Under standard lab conditions, both genes are dispensable for Drosophila development, but Nos appears to inhibit hyper-active behavior and fd59a appears to act in octopaminergic neurons to control egg-laying behavior. Together our data clarify the mechanisms through which Hb9 governs neuronal specification and differentiation and provide an initial characterization of the expression and function of Nos and fd59a in the Drosophila CNS. PMID:24512689

  2. Solar air-conditioning-active, hybrid and passive

    SciTech Connect

    Yellott, J. I.

    1981-04-01

    After a discussion of summer air conditioning requirements in the United States, active, hybrid, and passive cooling systems are defined. Active processes and systems include absorption, Rankine cycle, and a small variety of miscellaneous systems. The hybrid solar cooling and dehumidification technology of desiccation is covered as well as evaporative cooling. The passive solar cooling processes covered include convective, radiative and evaporative cooling. Federal and state involvement in solar cooling is then discussed. (LEW)

  3. Covering Climate Change in Wikipedia

    NASA Astrophysics Data System (ADS)

    Arritt, R. W.; Connolley, W.; Ramjohn, I.; Schulz, S.; Wickert, A. D.

    2010-12-01

    The first hit in an internet search for "global warming" using any of the three leading search engines (Google, Bing, or Yahoo) is the article "Global warming" in the online encyclopedia Wikipedia. The article garners about half a million page views per month. In addition to the site's visibility with the public, Wikipedia's articles on climate-related topics are widely referenced by policymakers, media outlets, and academia. Despite the site's strong influence on public understanding of science, few geoscientists actively participate in Wikipedia, with the result that the community that edits these articles is mostly composed of individuals with little or no expertise in the topic at hand. In this presentation we discuss how geoscientists can help shape public understanding of science by contributing to Wikipedia. Although Wikipedia prides itself on being "the encyclopedia that anyone can edit," the site has policies regarding contributions and behavior that can be pitfalls for newcomers. This presentation is intended as a guide for the geoscience community in contributing to information about climate change in this widely-used reference.

  4. Using Mustard Seed Meal and Cover Crops for Weed Control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There continues to be a steady growth in the use of fall planted brassica cover crops in the Columbia Basin especially prior to potatoes. Several benefits include better water infiltration, reclaiming nitrogen, reduced erosion, and suppression of nematodes, diseases, and weeds. Weed suppression is...

  5. 45 CFR 310.0 - What does this part cover?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... COMPUTERIZED TRIBAL IV-D SYSTEMS AND OFFICE AUTOMATION General Provisions § 310.0 What does this part cover... and Office Automation including: (a) The automated systems options for comprehensive Tribal IV-D... and Office Automation in § 310.15 of this part; (d) The conditions for funding the...

  6. 45 CFR 310.0 - What does this part cover?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... COMPUTERIZED TRIBAL IV-D SYSTEMS AND OFFICE AUTOMATION General Provisions § 310.0 What does this part cover... and Office Automation including: (a) The automated systems options for comprehensive Tribal IV-D... and Office Automation in § 310.15 of this part; (d) The conditions for funding the...

  7. 45 CFR 310.0 - What does this part cover?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... COMPUTERIZED TRIBAL IV-D SYSTEMS AND OFFICE AUTOMATION General Provisions § 310.0 What does this part cover... and Office Automation including: (a) The automated systems options for comprehensive Tribal IV-D... and Office Automation in § 310.15 of this part; (d) The conditions for funding the...

  8. 45 CFR 310.0 - What does this part cover?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... COMPUTERIZED TRIBAL IV-D SYSTEMS AND OFFICE AUTOMATION General Provisions § 310.0 What does this part cover... and Office Automation including: (a) The automated systems options for comprehensive Tribal IV-D... and Office Automation in § 310.15 of this part; (d) The conditions for funding the...

  9. 45 CFR 310.0 - What does this part cover?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... COMPUTERIZED TRIBAL IV-D SYSTEMS AND OFFICE AUTOMATION General Provisions § 310.0 What does this part cover... and Office Automation including: (a) The automated systems options for comprehensive Tribal IV-D... and Office Automation in § 310.15 of this part; (d) The conditions for funding the...

  10. 31 CFR 10.35 - Requirements for covered opinions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... a business purpose or that a transaction is potentially profitable apart from tax benefits. A.... A covered opinion is written advice (including electronic communications) by a practitioner... transaction under 26 CFR 1.6011-4(b)(2); (B) Any partnership or other entity, any investment plan...

  11. "The Northern Lights" Investigative Reporting Covers Deadly Decisions.

    ERIC Educational Resources Information Center

    Srour, George

    2001-01-01

    Presents a story package from the high school newspaper "The Northern Lights," called "Deadly Decisions." Includes an editorial, an eyewitness account, a timeline of the coverage itself, an interview with a local TV reporter, photographs, and a graph of the events the stories covered. (SR)

  12. Molecular genetic responses to lysergic acid diethylamide include transcriptional activation of MAP kinase phosphatase-1, C/EBP-beta and ILAD-1, a novel gene with homology to arrestins.

    PubMed

    Nichols, Charles D; Sanders-Bush, Elaine

    2004-08-01

    We recently demonstrated that the potent hallucinogenic drug lysergic acid diethylamide (LSD) dynamically influences the expression of a small collection of genes within the mammalian prefrontal cortex. Towards generating a greater understanding of the molecular genetic effects of hallucinogens and how they may relate to alterations in behavior, we have identified and characterized expression patterns of a new collection of three genes increased in expression by acute LSD administration. These genes were identified through additional screens of Affymetrix DNA microarrays and examined in experiments to assess dose-response, time course and the receptor mediating the expression changes. The first induced gene, C/EBP-beta, is a transcription factor. The second gene, MKP-1, suggests that LSD activates the MAP (mitogen activated protein) kinase pathway. The third gene, ILAD-1, demonstrates sequence similarity to the arrestins. The increase in expression of each gene was partially mediated through LSD interactions at 5-HT2A (serotonin) receptors. There is evidence of alternative splicing at the ILAD-1 locus. Furthermore, data suggests that various splice isoforms of ILAD-1 respond differently at the transcriptional level to LSD. The genes thus far found to be responsive to LSD are beginning to give a more complete picture of the complex intracellular events initiated by hallucinogens.

  13. Neoclassical Transport Including Collisional Nonlinearity

    SciTech Connect

    Candy, J.; Belli, E. A.

    2011-06-10

    In the standard {delta}f theory of neoclassical transport, the zeroth-order (Maxwellian) solution is obtained analytically via the solution of a nonlinear equation. The first-order correction {delta}f is subsequently computed as the solution of a linear, inhomogeneous equation that includes the linearized Fokker-Planck collision operator. This equation admits analytic solutions only in extreme asymptotic limits (banana, plateau, Pfirsch-Schlueter), and so must be solved numerically for realistic plasma parameters. Recently, numerical codes have appeared which attempt to compute the total distribution f more accurately than in the standard ordering by retaining some nonlinear terms related to finite-orbit width, while simultaneously reusing some form of the linearized collision operator. In this work we show that higher-order corrections to the distribution function may be unphysical if collisional nonlinearities are ignored.

  14. Investigating the impact of land cover change on peak river flow in UK upland peat catchments, based on modelled scenarios

    NASA Astrophysics Data System (ADS)

    Gao, Jihui; Holden, Joseph; Kirkby, Mike

    2014-05-01

    Changes to land cover can influence the velocity of overland flow. In headwater peatlands, saturation means that overland flow is a dominant source of runoff, particularly during heavy rainfall events. Human modifications in headwater peatlands may include removal of vegetation (e.g. by erosion processes, fire, pollution, overgrazing) or pro-active revegetation of peat with sedges such as Eriophorum or mosses such as Sphagnum. How these modifications affect the river flow, and in particular the flood peak, in headwater peatlands is a key problem for land management. In particular, the impact of the spatial distribution of land cover change (e.g. different locations and sizes of land cover change area) on river flow is not clear. In this presentation a new fully distributed version of TOPMODEL, which represents the effects of distributed land cover change on river discharge, was employed to investigate land cover change impacts in three UK upland peat catchments (Trout Beck in the North Pennines, the Wye in mid-Wales and the East Dart in southwest England). Land cover scenarios with three typical land covers (i.e. Eriophorum, Sphagnum and bare peat) having different surface roughness in upland peatlands were designed for these catchments to investigate land cover impacts on river flow through simulation runs of the distributed model. As a result of hypothesis testing three land cover principles emerged from the work as follows: Principle (1): Well vegetated buffer strips are important for reducing flow peaks. A wider bare peat strip nearer to the river channel gives a higher flow peak and reduces the delay to peak; conversely, a wider buffer strip with higher density vegetation (e.g. Sphagnum) leads to a lower peak and postpones the peak. In both cases, a narrower buffer strip surrounding upstream and downstream channels has a greater effect than a thicker buffer strip just based around the downstream river network. Principle (2): When the area of change is equal

  15. STEREOLOGICAL ESTIMATES OF THE BASAL FOREBRAIN CELL POPULATION IN THE RAT, INCLUDING NEURONS CONTAINING CHOLINE ACETYLTRANSFERASE (ChAT), GLUTAMIC ACID DECARBOXYLASE (GAD) OR PHOSPHATE-ACTIVATED GLUTAMINASE (PAG) AND COLOCALIZING VESICULAR GLUTAMATE TRANSPORTERS (VGluTs)

    PubMed Central

    GRITTI, I.; HENNY, P.; GALLONI, F.; MAINVILLE, L.; MARIOTTI, M.; JONES, B. E.

    2006-01-01

    The basal forebrain (BF) plays an important role in modulating cortical activity and influencing attention, learning and memory. These activities are fulfilled importantly yet not entirely by cholinergic neurons. Noncholinergic neurons also contribute and are comprised by GABAergic neurons and other possibly glutamatergic neurons. The aim of the present study was to estimate the total number of cells in the BF of the rat and the proportions of that total represented by cholinergic, GABAergic and glutamatergic neurons. For this purpose, cells were counted using unbiased stereological methods within the medial septum, diagonal band, magnocellular preoptic nucleus, substantia innominata and globus pallidus in sections stained for Nissl substance and/or the neurotransmitter enzymes, choline acetyltransferase (ChAT), glutamic acid decarboxylase (GAD) or phosphate-activated glutaminase (PAG). In Nissl-stained sections, the total number of neurons in the BF was estimated as ~355,000 and the numbers of ChAT-immuno-positive (+) as ~22,000, GAD+ ~119,000 and PAG+ ~316,000, corresponding to ~5%, ~35% and ~90% of the total. Thus, of the large population of BF neurons, only a small proportion has the capacity to synthesize acetylcholine (ACh), one third to synthesize GABA and the vast majority to synthesize glutamate (Glu). Moreover, through the presence of PAG, a proportion of ACh- and GABA-synthesizing neurons also have the capacity to synthesize Glu. In sections dual fluorescent immunostained for vesicular transporters, VGluT3 and not VGluT2 was present in the cell bodies of most PAG+ and ChAT+ and half the GAD+ cells. Given previous results showing that VGluT2 and not VGluT3 was present in BF axon terminals and not colocalized with VAChT or VGAT, we conclude that the BF cell population influences cortical and subcortical regions through neurons which release ACh, GABA or Glu from their terminals but which in part can also synthesize and release Glu from their soma or

  16. 31. SECTIONS AND DETAILS OF ARVFS FACILITY, INCLUDING RADIATION HAZARD ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. SECTIONS AND DETAILS OF ARVFS FACILITY, INCLUDING RADIATION HAZARD SIGN, WOOD RETAINING WALL, TANK COVER, AND DRAIN BOX. F.C. TORKELSON DRAWING NUMBER 842-ARVFS-701-3. INEL INDEX CODE NUMBER: 075 0701 851 151972. - Idaho National Engineering Laboratory, Advanced Reentry Vehicle Fusing System, Scoville, Butte County, ID

  17. Infliximab, adalimumab and golimumab for treating moderately to severely active ulcerative colitis after the failure of conventional therapy (including a review of TA140 and TA262): clinical effectiveness systematic review and economic model.

    PubMed Central

    Archer, Rachel; Tappenden, Paul; Ren, Shijie; Martyn-St James, Marrissa; Harvey, Rebecca; Basarir, Hasan; Stevens, John; Carroll, Christopher; Cantrell, Anna; Lobo, Alan; Hoque, Sami

    2016-01-01

    BACKGROUND Ulcerative colitis (UC) is the most common form of inflammatory bowel disease in the UK. UC can have a considerable impact on patients' quality of life. The burden for the NHS is substantial. OBJECTIVES To evaluate the clinical effectiveness and safety of interventions, to evaluate the incremental cost-effectiveness of all interventions and comparators (including medical and surgical options), to estimate the expected net budget impact of each intervention, and to identify key research priorities. DATA SOURCES Peer-reviewed publications, European Public Assessment Reports and manufacturers' submissions. The following databases were searched from inception to December 2013 for clinical effectiveness searches and from inception to January 2014 for cost-effectiveness searches for published and unpublished research evidence: MEDLINE, EMBASE, Cumulative Index to Nursing and Allied Health Literature, The Cochrane Library including the Cochrane Systematic Reviews Database, Cochrane Controlled Trials Register, Database of Abstracts of Reviews of Effects, the Health Technology Assessment database and NHS Economic Evaluation Database; ISI Web of Science, including Science Citation Index, and the Conference Proceedings Citation Index-Science and Bioscience Information Service Previews. The US Food and Drug Administration website and the European Medicines Agency website were also searched, as were research registers, conference proceedings and key journals. REVIEW METHODS A systematic review [including network meta-analysis (NMA)] was conducted to evaluate the clinical effectiveness and safety of named interventions. The health economic analysis included a review of published economic evaluations and the development of a de novo model. RESULTS Ten randomised controlled trials were included in the systematic review. The trials suggest that adult patients receiving infliximab (IFX) [Remicade(®), Merck Sharp & Dohme Ltd (MSD)], adalimumab (ADA) (Humira(®), Abb

  18. Can shrub cover increase predation risk for a desert rodent?

    USGS Publications Warehouse

    Schooley, R.L.; Sharpe, Peter B.

    1996-01-01

    Previous research indicates that predation risk may influence activity patterns, habitat partitioning, and community structure of nocturnal desert rodents. Shrub microhabitat is typically considered safer than open microhabitat for these small mammals. We investigated predation risk for Townsend's ground squirrels (Spermophilus townsendii), which are diurnal desert rodents that detect predators visually and use burrows for refuge. Our results suggested that shrub cover may increase risk for these squirrels by decreasing their ability to escape from predators. Our field experiment indicated that running speeds of juvenile squirrels were lower in shrub (Ceratoides lanata) habitat than in open areas. Shrub cover was also associated with shorter predator-detection distances (mammalian and avian) and fewer refuges (burrow entrances per hectare) than in open areas in one year but not in another. Our study demonstrated that the visual and locomotive obstruction of vegetative cover may increase predation risk for diurnal desert rodents and that elements of habitat-dependent risk may be temporally dynamic.

  19. Urban land cover classification using hyperspectral data

    NASA Astrophysics Data System (ADS)

    Hegde, G.; Ahamed, J. Mohammed; Hebbar, R.; Raj, U.

    2014-11-01

    Urban land cover classification using remote sensing data is quite challenging due to spectrally and spatially complex urban features. The present study describes the potential use of hyperspectral data for urban land cover classification and its comparison with multispectral data. EO-1 Hyperion data of October 05, 2012 covering parts of Bengaluru city was analyzed for land cover classification. The hyperspectral data was initially corrected for atmospheric effects using MODTRAN based FLAASH module and Minimum Noise Fraction (MNF) transformation was applied to reduce data dimensionality. The threshold Eigen value of 1.76 in VNIR region and 1.68 in the SWIR region was used for selection of 145 stable bands. Advanced per pixel classifiers viz., Spectral Angle Mapper (SAM) and Support Vector Machine (SVM) were used for general urban land cover classification. Accuracy assessment of the classified data revealed that SVM was quite superior (82.4 per cent) for urban land cover classification as compared to SAM (67.1 per cent). Selecting training samples using end members significantly improved the classification accuracy by 20.1 per cent in SVM. The land cover classification using multispectral LISS-III data using SVM showed lower accuracy mainly due to limitation of spectral resolution. The study indicated the requirement of additional narrow bands for achieving reasonable classification accuracy of urban land cover. Future research is focused on generating hyperspectral library for different urban features.

  20. 49 CFR 192.327 - Cover.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... soil Consolidated rock Inches (Millimeters) Class 1 locations 30 (762) 18 (457) Class 2, 3, and 4... least 24 inches (610 millimeters) of cover. (c) Where an underground structure prevents the installation.... (d) A main may be installed with less than 24 inches (610 millimeters) of cover if the law of...