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Sample records for activities ferc form

  1. 78 FR 24187 - Commission Information Collection Activities (FERC Form 6-Q); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... Energy Regulatory Commission Commission Information Collection Activities (FERC Form 6-Q); Comment... Energy Regulatory Commission (Commission or FERC) is submitting the information collection FERC Form 6-Q... issued a Notice in the Federal Register (78 FR 8500, 2/6/2013) requesting public comments. FERC...

  2. 76 FR 46781 - Commission Information Collection Activities (FERC Form 1 and FERC Form 1F); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ..., and 3Q \\2\\. The FERC Annual/Quarterly Report Forms provide the Commission, as well as others, with an... financial and operating report submitted for electric rate regulation and financial audits. Major is defined as having (1) One million Megawatt hours or more; (2) 100 megawatt hours of annual sales for...

  3. 78 FR 45919 - Commission Information Collection Activities (FERC Form 80); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ... the Federal Register (78 FR 28820, 5/16/2013) requesting public comments. FERC received no comments on... throughout the form. Specifically, FERC clarified and simplified instructions, removed redundancy in...

  4. 78 FR 8500 - Commission Information Collection Activities (FERC Form 6-Q); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... Financial Report of Oil Pipeline Companies. DATES: Comments on the collection of information are due April 8...: Title: FERC Form 6-Q (Quarterly Financial Report of Oil Pipeline Companies). OMB Control No.: 1902-0206... profitability, efficiency, risk and in its overall monitoring. Type of Respondents: Oil Pipelines. Estimate...

  5. 75 FR 5061 - Commission Information Collection Activities (FERC Form Nos. 6 and 6-Q); Comment Request; Extensions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ...''), ``Annual Report of Oil Pipeline Companies,'' implemented in 18 CFR Sections 357.1, 357.2, and 385.2011; OMB Control No. 1902-0022 and FERC Form 6-Q (``FERC-6Q'' or ``FERC-6-Q''), ``Quarterly Financial Report of Oil... ensure just and reasonable rates for transportation of crude oil and petroleum products by pipelines...

  6. 78 FR 24188 - Commission Information Collection Activities (FERC-500 and FERC-505); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... Energy Regulatory Commission Commission Information Collection Activities (FERC-500 and FERC- 505... Energy Regulatory Commission (Commission or FERC) is submitting the information collections Application for License/Relicense for Water Projects with Greater than 5 Megawatt Capacity (FERC-500),...

  7. 77 FR 73631 - Commission Information Collection Activities (FERC-500 and FERC-505); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ... Energy Regulatory Commission Commission Information Collection Activities (FERC-500 and FERC- 505... Energy Regulatory Commission (Commission or FERC) is submitting the information collections Application for License/Relicense for Water Projects with Greater than 5 Megawatt Capacity (FERC-500),...

  8. 75 FR 35003 - Commission Information Collection Activities (FERC Form No. 580); Request; Submitted for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... discriminatory to require transmission owners that own steam generation to report on their non-power tariffs while not requiring competing transmission owners that do not own steam generation over 50MW to do so... the Federal Register (74 FR 66114, 12/14/2009) requesting public comments. FERC received comments...

  9. 75 FR 15424 - Commission Information Collection Activities (FERC-538, FERC-539, FERC-577, FERC-606, and FERC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... issued a Notice in the Federal Register (75 FR 2124, 1/ 14/2010) requesting public comments. FERC... clearance numbers will continue to remain separate and distinct. FERC-538. Under the Natural Gas Act (NGA..., a natural gas pipeline company may be ordered by the Commission to extend or improve...

  10. 77 FR 77067 - Commission Information Collection Activities (FERC Form No. 556); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ...-8659 for TTY. Docket: Users interested in receiving automatic notification of activity in this docket... transmission services priced on a nondiscriminatory basis; Operate in ``parallel'' with other...

  11. 78 FR 24402 - Commission Information Collection Activities (FERC Form 80); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-25

    ... responses response burden (A) (B) (A) x (B) = (D) (C) x (D) (C) 400 0.167 66.8 3 200 ] The total estimated... unconstructed project shall file an initial Form No. 80 after such project has been in operation for a full calendar year prior to the filing deadline. Each licensee of an existing (constructed) project shall...

  12. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Annual reports of centralized service companies, and FERC-61, Narrative description of service company... companies, and FERC-61, Narrative description of service company functions. (a) General. (1) FERC Form No... 1 each year thereafter, a narrative description, FERC-61, of the service company's functions...

  13. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Annual reports of centralized service companies, and FERC-61, Narrative description of service company... companies, and FERC-61, Narrative description of service company functions. (a) General. (1) FERC Form No... 1 each year thereafter, a narrative description, FERC-61, of the service company's functions...

  14. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Annual reports of centralized service companies, and FERC-61, Narrative description of service company... companies, and FERC-61, Narrative description of service company functions. (a) General. (1) FERC Form No... 1 each year thereafter, a narrative description, FERC-61, of the service company's functions...

  15. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual reports of centralized service companies, and FERC-61, Narrative description of service company... companies, and FERC-61, Narrative description of service company functions. (a) General. (1) FERC Form No... 1 each year thereafter, a narrative description, FERC-61, of the service company's functions...

  16. 75 FR 2124 - Commission Information Collection Activities (FERC-538, FERC-539, FERC-577, FERC-606, and FERC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-14

    ..., such as administrative costs and the cost for information technology. Indirect or overhead costs are... burdens and the associated public costs follow. Number of Projected average FERC Data collection Projected... rounding and/or truncating. The total annual cost to respondents 1 2 is estimated as follows....

  17. 75 FR 70726 - Commission Information Collection Activities (FERC-511 and FERC-515); Comment Request; Submitted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-18

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC-511 and FERC- 515); Comment Request; Submitted for OMB Review November 10, 2010. AGENCY: Federal Energy Regulatory...

  18. 77 FR 56636 - Commission Information Collection Activities (FERC-500 & FERC-505); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC-500 & FERC- 505); Comment Request; Extension AGENCY: Federal Energy Regulatory Commission. ACTION: Notice of information...

  19. 78 FR 9679 - Commission Information Collection Activities (FERC-60, FERC-61, & FERC-555A); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-11

    ... (Preservation of Records Companies and Service Companies Subject to PUHCA. OMB Control No.: 1902-0215. Type of... information collections [FERC-60 (Annual Report of Centralized Service Companies), FERC-61 (Narrative Description of Service Company Functions), and FERC- 555A (Preservation of Records Companies and...

  20. 77 FR 8245 - Commission Information Collection Activities (FERC-65, FERC-65A, and FERC-65B); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-14

    ... Control Number. This should simplify the FERC administrative burden of processing these information... Notification 0 1 0 1 0 Total \\3\\ N/A \\3\\ N/A 9 \\3\\ N/A 25 The total estimated annual cost burden to respondents... employee. The estimated annual cost of filing the FERC-65, FERC-65A, and FERC-65B per response is...

  1. 78 FR 44424 - Revisions to Page 700 of FERC Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-24

    ... rate base, and income taxes reported on Page 700. \\8\\ Revisions to Page 700 of FERC Form No. 6, 77 FR... Stats. & Regs. ] 32,692 at P 9 n.13 (citing ARCO Pipe Line Co., Opinion No. 351-A, 53 FERC ] 61,398...

  2. 77 FR 40347 - Commission Information Collection Activities (FERC-552); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-09

    ... the Federal Register (77 FR 19009, 3/29/2012) requesting public comments. FERC received no comments on... without taking on the risks of active trading. FERC-552 had its genesis in the Energy Policy Act of...

  3. 75 FR 51027 - Commission Information Collection Activities (FERC-917 and FERC-918) 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... issued a Notice in the Federal Register (75 FR 30008, 5/ 28/2010) requesting public comments. FERC...; Recovery of Stranded Costs by Public Utilities and Transmitting Utilities, Order No. 888, 61 FR 21540 (May 10, 1996), FERC Stats. & Regs. ] 31,036 (1996), order on reh'g, Order No. 888-A, 62 FR 12274 (Mar....

  4. 75 FR 7466 - Commission Information Collection Activities (FERC-912 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-19

    ... Notice February 3, 2010. AGENCY: Federal Energy Regulatory Commission. \\1\\ FERC-912 (``Cogeneration and... FERC) has submitted the FERC-912 \\1\\ to the Office of Management and Budget (OMB) for review of the... Docket No. IC09-912-001 requesting public comment (74 FR 63743, 12/4/ 2009), FERC and OMB received...

  5. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Quarterly report of oil pipeline companies. 357.4 Section 357.4 Conservation of Power and Water Resources... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  6. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Quarterly report of oil pipeline companies. 357.4 Section 357.4 Conservation of Power and Water Resources... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  7. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Quarterly report of oil pipeline companies. 357.4 Section 357.4 Conservation of Power and Water Resources... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  8. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Quarterly report of oil pipeline companies. 357.4 Section 357.4 Conservation of Power and Water Resources... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  9. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Quarterly report of oil pipeline companies. 357.4 Section 357.4 Conservation of Power and Water Resources... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  10. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  11. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 715... Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER... transmitting utility, as defined in § 3(23) of the Federal Power Act, that operates integrated (that is,...

  12. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  13. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  14. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  15. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  16. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  17. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  18. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  19. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  20. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  1. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Report of Oil Pipeline Companies. 357.2 Section 357.2 Conservation of Power and Water Resources FEDERAL.... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... the Commission copies of FERC Form No. 6, “Annual Report of Oil Pipeline Companies,” pursuant to...

  2. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Report of Oil Pipeline Companies. 357.2 Section 357.2 Conservation of Power and Water Resources FEDERAL.... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... the Commission copies of FERC Form No. 6, “Annual Report of Oil Pipeline Companies,” pursuant to...

  3. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Report of Oil Pipeline Companies. 357.2 Section 357.2 Conservation of Power and Water Resources FEDERAL.... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... the Commission copies of FERC Form No. 6, “Annual Report of Oil Pipeline Companies,” pursuant to...

  4. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Report of Oil Pipeline Companies. 357.2 Section 357.2 Conservation of Power and Water Resources FEDERAL.... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... the Commission copies of FERC Form No. 6, “Annual Report of Oil Pipeline Companies,” pursuant to...

  5. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Report of Oil Pipeline Companies. 357.2 Section 357.2 Conservation of Power and Water Resources FEDERAL.... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... the Commission copies of FERC Form No. 6, “Annual Report of Oil Pipeline Companies,” pursuant to...

  6. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 561, Annual report of interlocking positions. 131.31 Section 131.31 Conservation of Power and Water Resources... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR...

  7. 78 FR 16487 - Commission Information Collection Activities (FERC-556); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ..., ``Certification of Qualifying Facility (QF) Status for a Small Power Production or Cogeneration Facility'' (Form No. 556), to the Office of Management and Budget (OMB) for review of the information collection... FR 77067, 12/31/2012) requesting public comments. FERC received no comments on the FERC-556 and...

  8. 76 FR 58803 - Commission Information Collection Activities (FERC-725A); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-22

    ... FR 23801, 04/28/2011) requesting public comments. FERC received no comments on the FERC-725A and has....asp , to establish a username and password before eFiling. The Commission will send an automatic... Street, NE., Washington, DC 20426. Users interested in receiving automatic notification of activity...

  9. 78 FR 7767 - Commission Information Collection Activities (FERC-729); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-04

    ... Transmission Facilities), to the Office of Management and Budget (OMB) for review of the information collection... FR 67638, 11/13/2012) requesting public comments. FERC received no comments on the FERC-729 and is... interested in receiving automatic notification of activity in this docket or in viewing/downloading...

  10. 78 FR 16485 - Commission Information Collection Activities (FERC-607); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... issued a Notice in the Federal Register (77 FR 76015, December 26, 2012) requesting public comments. FERC... (EPAct 2005) modified FERC's role in order to better coordinate the activities of the separate agencies with varying responsibilities over proposed natural gas projects. Section 313 of EPAct 2005 \\1\\...

  11. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... company system, including a special-purpose company (e.g., a fuel supply company or a construction company..., Annual reports of centralized service companies, and FERC-61, Narrative description of service company... COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE PUBLIC UTILITY HOLDING COMPANY ACT OF 2005,...

  12. 77 FR 35667 - Commission Information Collection Activities (FERC-567); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC-567); Comment Request AGENCY: Federal Energy Regulatory Commission, Energy. ACTION: Comment request. SUMMARY: In...

  13. 77 FR 6550 - Commission Information Collection Activities (FERC-725A); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-08

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC-725A); Comment Request AGENCY: Federal Energy Regulatory Commission, DOE. ACTION: Comment request. SUMMARY: In compliance...

  14. 77 FR 76015 - Commission Information Collection Activities (FERC-607); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-26

    ... (EPAct 2005) modified FERC's role in order to better coordinate the activities of the separate agencies with varying responsibilities over proposed natural gas projects. Section 313 of EPAct 2005 \\1\\...

  15. 76 FR 66707 - Commission Information Collection Activities (FERC-1 and FERC-1F); Comment Request; Submitted for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-27

    ... accounting records using dissimilar accounting methods and classifications to record similar transactions and... Register (76 FR 46781, 10/3/2011) requesting public comments. FERC received one comment on the FERC-1 and... provided by the Commission's chart of accounts and related accounting instructions permits...

  16. 18 CFR 141.2 - FERC Form No. 1-F, Annual report for Nonmajor public utilities and licensees.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual report for Nonmajor public utilities and licensees. 141.2 Section 141.2 Conservation of Power and... ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978 STATEMENTS AND REPORTS (SCHEDULES) § 141.2 FERC Form No. 1-F, Annual report for Nonmajor public utilities and licensees. (a) Prescription. The form...

  17. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY...

  18. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  19. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  20. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  1. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  2. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  3. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  4. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  5. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  6. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  7. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  8. 76 FR 28760 - Commission Information Collection Activities (FERC-73); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-18

    .... SUPPLEMENTARY INFORMATION: The information collected under the requirements of FERC Form No. 73 ``Oil Pipelines... Order No. 12009, 42 FR 46277 (September 13, 1977). The Commission has authority over interstate oil... necessary for the subsequent investigation and review of an oil pipeline company's proposed...

  9. 75 FR 44781 - Commission Information Collection Activities (FERC-582); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-29

    ...;'' OMB Control No. 1902-0132) covers the filing ] requirements in the Code of Federal Regulations (CFR..., the Omnibus Budget Reconciliation Act of 1986 (OBRA) (42 U.S.C. 7178) authorizes the Commission ``to... (Annual Charges),'' OMB Control Number 1902-0136) for hydropower generation facilities; (b) FERC Form...

  10. 18 CFR 141.1 - FERC Form No. 1, Annual report of Major electric utilities, licensees and others.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 1, Annual report of Major electric utilities, licensees and others. 141.1 Section 141.1 Conservation of Power and... instrumentality engaged in generation, transmission, distribution, or sale of electric energy, however...

  11. 18 CFR 141.1 - FERC Form No. 1, Annual report of Major electric utilities, licensees and others.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 1, Annual report of Major electric utilities, licensees and others. 141.1 Section 141.1 Conservation of Power and... instrumentality engaged in generation, transmission, distribution, or sale of electric energy, however...

  12. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric...

  13. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric...

  14. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric...

  15. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  16. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  17. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  18. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  19. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  20. 78 FR 58295 - Commission Information Collection Activities (FERC-725A); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-23

    ... Reliability Standards for the Bulk Power System, to the Office of Management and Budget (OMB) for review of... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC-725A); Comment...

  1. 77 FR 31607 - Commission Information Collection Activities (FERC-587); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... Survey System Lands in Public Land States]) to the Office of Management and Budget (OMB) for review of... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC-587); Comment...

  2. 78 FR 53742 - Commission Information Collection Activities (Ferc-582); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-30

    ... the Federal Register (78 FR 47310, 8/5/2013) requesting public comments. FERC received no comments on... ``Estimate of Annual Burden'' section of this notice. \\1\\ 78 FR 30912, 5/23/2013; and 78 FR 47310, 8/5/2013... automatic notification of activity in this docket or in viewing/downloading comments and issuances in...

  3. 75 FR 7465 - Commission Information Collection Activities (FERC-730); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-19

    ... in response to the Federal Register notice (74 FR 59146, 11/17/2009) and has noted that in its... an automatic acknowledgement to the sender's e-mail address upon receipt of comments. All comments... INFORMATION: The FERC-730, ``Report of Transmission Investment Activity'' (OMB Control No. 1902-0239) is...

  4. 77 FR 53877 - Commission Information Collection Activities (FERC-715); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    ... public comment on the currently approved information collection, Annual Transmission Planning and...-8659 for TTY. Docket: Users interested in receiving automatic notification of activity in this docket...: Title: FERC-715: Annual Transmission Planning and Evaluation Report. OMB Control No.: 1902-0171. Type...

  5. 77 FR 58115 - Commission Information Collection Activities (FERC-546); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-19

    ... jurisdictional transportation, natural gas storage, and unbundled sales activities of interstate natural gas...-of-state gas from entities within or at the boundary of a state if all the natural gas so received is... pertinent state agencies rather than by FERC. The Natural Gas Act section 1(c) exempts Hinshaw...

  6. 78 FR 13657 - Commission Information Collection Activities (FERC-577); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... currently approved information collection, FERC-577, Natural Gas Facilities: Environmental Review and Compliance (formerly Gas Pipeline Certificates: Environmental Impact Statement). The FERC is proposing to... Code of Federal Regulations 1320.3. FERC-577--Natural Gas Facilities: Environmental Review...

  7. 75 FR 71097 - Commission Information Collection Activities (FERC-919); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... Public Utilities, Order No. 697, 72 FR 39,904 (Jul. 20, 2007), FERC Stats. & Regs. ] 31,252 (2007), clarified, 121 FERC ] 61,260 (2007), order on reh'g, Order No. 697- A, 73 FR 25832 (May 7, 2008), FERC Stats. & Regs. ] 31,268, Order No. 697-B, 73 FR 79610 (Dec. 30, 2008), FERC Stats. & Regs. ] 31,285...

  8. 77 FR 71186 - Commission Information Collection Activities (FERC-912); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-29

    ... Commission (Commission or FERC) is submitting the information collection FERC-912, Cogeneration and Small... Purchase or Sell, to the Office of Management and Budget (OMB) for review of the information collection... FR 58365, 09/20/2012) requesting public comments. FERC received no comments on the FERC-912 and...

  9. 77 FR 64495 - Commission Information Collection Activities (FERC-922); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-22

    ... issued a Notice in the Federal Register (77 FR 12832, 3/2/2012) requesting public comments. FERC received...Library system (at http://www.ferc.gov/docs-filing/elibrary.asp ) by searching under Docket No....

  10. 78 FR 66350 - Commission Information Collection Activities (FERC-574); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... FR 49491, 8/14/2013) requesting public comments. FERC received no comments on the FERC-574 and is... Commission; and (d) that such State Commission is exercising that jurisdiction. 18 CFR Part 152 specifies...

  11. 77 FR 76016 - Commission Information Collection Activities (FERC-606); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-26

    ... responsibilities over proposed natural gas projects, the Energy Policy Act of 2005 (EPAct 2005) modified FERC's role. Section 313 of EPAct 2005 \\1\\ directs FERC: (1) to establish a schedule for agencies to...

  12. 76 FR 52647 - Commission Information Collection Activities (FERC-583); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... FR 34689, 06/14/2011) requesting public comments. FERC received no comments on the FERC-583 and is... government administrative costs and for the use of government dams. The Commission implements theses...

  13. 76 FR 35872 - Commission Information Collection Activities (Ferc-603); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... FR 18743, 4/ 5/2011) requesting public comments. FERC received no comments on the FERC-603 and has... 21, 2003, the Commission issued Order No. 630 (66 FR 52917) to address the appropriate treatment of.... Critical infrastructure means existing and proposed systems and assets, whether physical or virtual,...

  14. 78 FR 58296 - Commission Information Collection Activities (FERC-604); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-23

    ... Respondents: Public utilities and licensees, natural gas companies, and oil pipeline companies. Estimate of... FR 29359, 5/20/2013) requesting public comments. FERC received no comments on the FERC-604 and is... more than $1 billion) between regulated pipeline affiliates and non-regulated parents whose...

  15. 76 FR 18209 - Commission Information Collection Activities (FERC-725D); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... FR 71678, 11/24/2010) requesting public comments. FERC received no comments on the FERC-725D and has... Mandatory Reliability Standards for the Bulk-Power System, Order No. 693, 72 FR, 16,416 (April 4, 2007), 118... methodologies to determine system operating limits (SOLs) for the bulk- power system in the planning...

  16. 77 FR 28861 - Commission Information Collection Activities (FERC-585); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ..., Reporting of Electric Energy shortages and Contingency Plans under PURPA, to the Office of Management and... fax at (202) 273-0873. SUPPLEMENTARY INFORMATION: Title: FERC-585, Reporting of Electric Energy.... Abstract: The information collected under the requirements of FERC- 585, ``Reporting of Electric...

  17. 76 FR 64932 - Commission Information Collection Activities (FERC-510); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-19

    ... estimated annual cost per respondent is $685 (rounded). The reporting burden includes the total time, effort... FR 43996, 07/22/2011) requesting public comments. FERC received no comments on the FERC-510 and has... electronically, access the Commission's Web site and click on Documents & Filing, E-Filing (...

  18. 77 FR 16214 - Commission Information Collection Activities (FERC-598); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ... Energy Regulatory Commission (Commission or FERC) is submitting the information collection FERC-598, Self... as explained below. The Commission issued a Notice in the Federal Register (77 FR 97, 1/3/2012...: Comments filed with OMB, identified by the OMB Control No. 1902-0166, should be sent via email to...

  19. 78 FR 16486 - Commission Information Collection Activities (FERC-606); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... explained below. The Commission issued a Notice in the Federal Register (77 FR 76016, 12/26/2012) requesting... varying responsibilities over proposed natural gas projects, the Energy Policy Act of 2005 (EPAct 2005) modified FERC's role. Section 313 of EPAct 2005 \\1\\ directs FERC to establish a schedule for agencies...

  20. 77 FR 39691 - Commission Information Collection Activities (FERC-725); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... Organization; Procedures for Electric Reliability Standards, to the Office of Management and Budget (OMB) for... issued a Notice in the Federal Register (77 FR 24189, 04/23/2012) requesting public comments. FERC...: Title: FERC-725, Certification of Electric Reliability Organization; Procedures for Electric...

  1. 77 FR 46086 - Commission Information Collection Activities (FERC-576); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-02

    ... Register (77 FR 28369, May 14, 2012) requesting public comments. FERC received no comments on the FERC-576... reporting requirements. Abstract: A natural gas company must obtain Commission authorization to engage in the transportation, sale, or exchange of natural gas in interstate commerce under the Natural Gas...

  2. 76 FR 66920 - Commission Information Collection Activities (FERC-549); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-28

    ... wage statistics for staff in the areas of management, business and financial, legal and administrative... described below to the Office of Management and Budget (OMB) for review of the information collection... FR 46783, 08/3/2011) requesting public comments. FERC received no comments on the FERC-549 and...

  3. 75 FR 5063 - Commission Information Collection Activities (FERC-729); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ..., alternatives other than new transmission lines. h. Comment: Utilities currently lack commitment to work with... comments in response to the Federal Register notice (74 FR 52796, 10/14/2009). FERC has summarized...

  4. 78 FR 30912 - Commission Information Collection Activities (FERC-582); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    .... \\5\\ 18 CFR 382.201. Respondents file requests for waivers and exemptions of fees and charges \\6... respondent responses response annual burden (A) (B) (A) x (B) = (C) (D) (C) x (D) FERC-582 \\8\\ 114 1 114...

  5. 77 FR 97 - Commission Information Collection Activities, Proposed Collection (FERC-598); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... Holding Company Act of 2005, 70 FR. 75,592 (2005), FERC Statutes and Regulations ] 31,197 (2005) Order on reh'g, 71 FR 28,446 (2006), FERC Statutes and Regulations ] 31,213 (2006), order on reh'g, 71 FR 42... statutory provisions of Title XII, subchapter F of the Energy Policy Act of 2005 (EPAct 2005).\\1\\ \\1\\...

  6. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to... form. (d) Critical Energy Infrastructure Information. (1) If the instructions in Form No. 715 require a utility to reveal Critical Energy Infrastructure Information (CEII), as defined in § 388.113(c) of...

  7. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to... form. (d) Critical Energy Infrastructure Information. (1) If the instructions in Form No. 715 require a utility to reveal Critical Energy Infrastructure Information (CEII), as defined in § 388.113(c) of...

  8. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to... form. (d) Critical Energy Infrastructure Information. (1) If the instructions in Form No. 715 require a utility to reveal Critical Energy Infrastructure Information (CEII), as defined in § 388.113(c) of...

  9. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to... form. (d) Critical Energy Infrastructure Information. (1) If the instructions in Form No. 715 require a utility to reveal Critical Energy Infrastructure Information (CEII), as defined in § 388.113(c) of...

  10. 76 FR 4888 - Commission Information Collection Activities (FERC-537); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-27

    ...In compliance with the requirements of section 3507 of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507, the Federal Energy Regulatory Commission (Commission or FERC) has submitted the information collection described below to the Office of Management and Budget (OMB) for review of the information collection requirements. Any interested person may file comments directly with OMB and should......

  11. 76 FR 52646 - Commision Information Collection Activities [FERC-588]; Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... company must obtain Commission approval to engage in the transportation, sale or exchange of natural gas... to the Commission as explained below. The Commission issued a Notice in the Federal Register (76 FR.... SUPPLEMENTARY INFORMATION: The information collected under the requirements of FERC-588, ``Emergency Natural...

  12. 76 FR 30928 - Commision Information Collection Activities (FERC-588), Proposed Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... information collected under the requirements of FERC-588, ``Emergency Natural Gas Transportation, Sale and... company must obtain Commission approval to engage in the transportation, sale or exchange of natural gas... provisions of Sections 7(c) of the Natural Gas Act (NGA) (Pub. L. 75-688) (15 U.S.C. 717-717w) and...

  13. 76 FR 76702 - Commission Information Collection Activities, Proposed Collection (FERC-550); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-08

    ... information collected under the requirements of FERC-550, ``Oil Pipeline Rates: Tariff Filings'' (OMB No. 1902...) (Pub. L. 337, 34 Stat. 584). Jurisdiction over oil pipelines as it relates to the establishment of... requirements for proposed oil pipeline rates. The data that oil pipelines file is the basis for...

  14. 78 FR 77111 - Commission Information Collection Activities (FERC-555); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-20

    ... public utilities, natural gas and oil pipeline companies subject to the Commission's jurisdiction... as well as natural gas and oil pipeline companies. These changes included revising the general.... FERC-555--Preservation of Records for Public Utilities and Licenses, Natural Gas and Oil...

  15. 77 FR 28863 - Commission Information Collection Activities (FERC-915); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ... figure on the estimated cost to service and to store 1 GB of data (based on the aggregated cost of an IBM advanced data protection server). Comments: Comments are invited on: (1) Whether the collection of... may be reached by email at DataClearance@FERC.gov , telephone at (202) 502-8663, and fax at (202)...

  16. 77 FR 2963 - Commission Information Collection Activities (FERC-725F); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... issued a Notice in the Federal Register (76 FR 67158, 10/31/2011) requesting public comments. FERC... Power Act (FPA) (16 U.S.C. 824o). On August 8, 2005, the Electricity Modernization Act of 2005, which is... ERO and approved by the Commission apply to users, owners and operators of the Bulk-Power System...

  17. 78 FR 63974 - Commission Information Collection Activities (FERC-547); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-25

    ... information collection requirements. Any interested person may file comments directly with OMB and should... the Federal Register (78 FR 43186, 7/19/2013) requesting public comments. FERC received no comments on... annual burden (A) (B) (A)x(B)=(C) (D) (C)x(D) Natural gas companies 11 1 11 75 825 The total...

  18. 77 FR 77069 - Commission Information Collection Activities (FERC-730); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    .... Specifically, in accordance with the statute, public utilities with incentive rates must file: \\1\\ Energy.../2006), published: 71 FR 43294. Actual transmission investment for the most recent calendar year, and... 18 CFR 35.35(h) must file the FERC-730. Estimate of Annual Burden \\3\\: The Commission estimates...

  19. 75 FR 34107 - Commission Information Collection Activities (FERC-547); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... administrative costs and the cost for information technology. Indirect or overhead costs are costs incurred by an... (1) (2) (3) (1) x (2) x (3) FERC-547 30 1 75 2,250 The total estimated annual cost burden to... information; and (7) transmitting or otherwise disclosing the information. The estimate of cost...

  20. 76 FR 30927 - Commission Information Collection Activities (FERC-516A), Proposed Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... administrative costs and the cost for information technology. Indirect or overhead costs are costs incurred by an... Interconnection Agreements and Procedures, Order No. 2006, 70 FR 34189 (May 12, 2005), FERC Stats. & Regs. ] 31...-conforming interconnection procedures and agreements that occur on occasion. The estimated total cost...

  1. 75 FR 64301 - Commission Information Collection Activities (FERC-537); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-19

    ... to providing this information, such as administrative costs and the cost for information technology... presenting the basis for the proposed action; and Cost of the proposed facilities, plans for financing, and...) FERC-537 225 3.44 133 102,942 The total estimated annual cost burden to respondents is $6,823,570...

  2. 75 FR 47804 - Commission Information Collection Activities (FERC-574); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... administrative costs and the cost for information technology. Indirect or overhead costs are costs incurred by an...) response (3) burden hours (1)x(2)x(3) FERC-574 1 1 60 60 The total estimated annual cost burden to... an employee works each year. \\2\\ Estimated average annual cost per employee. The reporting...

  3. 75 FR 41840 - Commission Information Collection Activities (FERC-919); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... Public Utilities, Order No. 697, 72 FR 39,904 (Jul. 20, 2007), FERC Stats. & Regs. ] 31,252 (2007) (Final Rule). Market power analyses must address both horizontal and vertical market power. To demonstrate... A of Subpart H and include all materials referenced. To demonstrate a lack of vertical market...

  4. 78 FR 62006 - Commission Information Collection Activities (FERC-919); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-11

    ... Public Utilities, Order No. 697, 72 FR 39904 (Jul. 20, 2007), FERC Stats. & Regs. ] 31,252 (2007) (Final Rule). Horizontal Market Power Analysis Market power analyses must address both horizontal and vertical... A of Subpart H and include all materials referenced. Vertical Market Power Analysis To demonstrate...

  5. 75 FR 56082 - Commission Information Collection Activities (FERC-725E); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-15

    ... Record-keeping Balancing Authorities 68 Generator Operators 206 Load-Serving Entities 149 Transmission... the Federal Register (75 FR 35002, 6/ 21/2010) requesting public comments. FERC received no comments...).\\3\\ \\1\\ 16 U.S.C. 824o(e)(4). \\2\\ 16 U.S.C. 824o(a)(7) and (e)(4). \\3\\ 72 FR 33462, June 18,...

  6. 77 FR 31342 - Commission Information Collection Activities (FERC-914); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-25

    ... Production and Cogeneration Facilities, Order No. 671, 71 FR 7852 (2/15/2006), FERC Stats. & Regs. ] 31,203... and its continuing obligation to review its policies encouraging cogeneration and small power... soliciting public comment on the currently approved information collection, Cogeneration and Small...

  7. 78 FR 47310 - Commission Information Collection Activities (FERC-582); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-05

    ... the Federal Register (78 FR 30912, 5/23/2013) requesting public comments. FERC received no comments on... information are due by September 4, 2013. ADDRESSES: Comments filed with OMB, identified by the OMB Control No...: Title: Electric Fees, Annual Charges, Waivers, and Exemptions. OMB Control No.: 1902-0132. Type...

  8. 75 FR 61455 - Commission Information Collection Activities (FERC-582); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... the Federal Register (75 FR 44781, 07/29/2010) requesting public comments. FERC received comments from... oira_submission@omb.eop.gov and include OMB Control Number 1902-0132 for reference. The Desk Officer...-582, ``Electric Fees; Annual Charges; Waivers; and Exemptions;'' OMB Control No. 1902-0132, covers...

  9. 76 FR 46783 - Commission Information Collection Activities (FERC-549); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... use in price indices.'' FERC uses these records to monitor the jurisdictional transportation... information about natural gas pipelines' ability to affect the prices shippers can obtain from consumers... orders to which the price data may be relevant. \\2\\ 18 CFR 1c.1 and 1c.2, 71 FR 4,244 (2006). Failure...

  10. 75 FR 65618 - Commission Information Collection Activities (FERC-725B); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-26

    ... (2) x (3) FERC-725B Estimate of U.S. Entities that have 345 1 320 110,400 identified Critical Cyber Assets Estimate of U.S. Entities that have not 1,156 1 8 9,248 identified Critical Cyber Assets Totals 1...(c)(2)(A) of the Paperwork Reduction Act of 1995, 44 U.S.C. 3506(c)(2)(A) (2006), (Pub. L....

  11. 76 FR 30930 - Commission Information Collection Activities (FERC-512); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... surveys to prepare maps, plans, specifications and estimates; conducts engineering, economic and..., mechanical, or other ] technological collection techniques or other forms of information technology,...

  12. 75 FR 57744 - Commission Information Collection Activities (FERC-555); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-22

    ... transferred from one media to another with no loss of data would exceed the record period. On January 27, 2000..., mechanical, or other technological collection techniques or other forms of information technology...

  13. 78 FR 63172 - Commission Information Collection Activities (FERC-733); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-23

    ... Force at NERC developed DADS to collect demand response program information. DADS currently collects..., fillable form that will include such user friendly features as pre-populated fields and drop-down...

  14. 77 FR 28369 - Commission Information Collection Activities (FERC-576); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-14

    ...) serious interruptions to service, and (3) damage to jurisdictional natural gas facilities due to natural disaster or terrorist activity, that creates the potential for serious delivery problems on the pipeline's... damaged; (4) The time the service interruption or damage to the facilities occurred; (5) The...

  15. 78 FR 11167 - Commission Information Collection Activities (FERC-592); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-15

    ... respondents to monitor the pipeline's transportation, sales, and storage activities for its marketing... the requirements of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507(a)(1)(D), the Federal Energy... Conduct for Transmission Provider; and Marketing Affiliates of Interstate Pipelines), to the Office...

  16. 76 FR 67158 - Commission Information Collection Activities (FERC-725F); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ....asp , to establish a user name and password before eFiling. The Commission will send an automatic... NE., Washington, DC 20426. Users interested in receiving automatic notification of activity in this... Standard NUC-001-2 is to require ``coordination between nuclear plant generator operators and...

  17. 77 FR 16215 - Commission Information Collection Activities (FERC-716); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ... as explained below. The Commission issued a Notice in the Federal Register (77 FR 98, 1/3/2012... receiving automatic notification of activity in this docket or in viewing/downloading comments and issuances... Transmission Service and Response by Transmitting Utility Under Sections 211(a) and 213(a) of the Federal...

  18. 18 CFR 131.80 - FERC Form No. 556, Certification of qualifying facility (QF) status for a small power production...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Certification of qualifying facility (QF) status for a small power production or cogeneration facility. 131.80 Section 131.80 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978...

  19. 75 FR 21623 - Commission Information Collection Activities (FERC Form Nos. 6 and 6-Q); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-26

    ...''), ``Annual Report of Oil Pipeline Companies,'' implemented in 18 CFR Sections 357.1, 357.2, and 385.2011; OMB... of Oil Pipeline Companies,'' implemented in 18 CFR Section 357.4; OMB Control No. 1902-0206. The... Value Put-In Place (VPIP) for oil pipeline utilities. Census VPIP serves as a major source data input...

  20. 78 FR 26766 - Commission Information Collection Activities (FERC Form 580); Comment Request; Revision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-08

    ... requested information. Report response to any part of Question 6 for the data individually for each fuel oil... response to any part generation and thermal energy of Question 6 for fuel oil no. associated with the..., natural gas, and oil should be power generation and thermal reported. Information for ALL FUELS...

  1. 78 FR 25431 - Revised Information Collection Activities (FERC-545, FERC-549, and FERC-550); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... procedures to interstate and intrastate natural gas pipelines and interstate oil pipelines. The Commission... oil pipelines. These procedures will replace the use of random-number generated passwords to... revised procedures, each regulated natural gas pipeline and oil pipeline will be able to maintain a...

  2. Test for abuse under NGPA Section 601(c)(2): Office of Consumers' Counsel v. FERC

    SciTech Connect

    Not Available

    1987-01-01

    A review of fraud and abuse challenge against the Natural Gas Policy Act (NGPA) section 601, which allows the Federal Energy Regulatory Commission to control the prices pipeline companies pay. The author explores the statutory background of the case, FERC's authority to interpret provisions of the NGPA in the area of take-or-pay problems, and court opinions. He concludes by outlining approaches for applying the fraud and abuse test, and views the guaranteed pass-through exception of fraud, abuse, or similar grounds as a valuable enforcement tool for FERC. As the terms become better defined, pipelines will be able to structure their business activities to avoid situations likely to lead to fraud or abuse charges. Both FERC and the natural gas industry will benefit from further development of the standard.

  3. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS...

  4. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS...

  5. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS...

  6. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS...

  7. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS...

  8. 18 CFR 366.4 - FERC-65, notification of holding company status, FERC-65A, exemption notification, and FERC-65B...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... OF ENERGY REGULATIONS UNDER THE PUBLIC UTILITY HOLDING COMPANY ACT OF 2005, FEDERAL POWER ACT AND... the Natural Gas Act § 366.4 FERC-65, notification of holding company status, FERC-65A, exemption... meet the definition of a holding company as provided by § 366.1 as of February 8, 2006 shall notify...

  9. $sup 238$Pu fuel form activities

    SciTech Connect

    Not Available

    1987-06-01

    This report for STYPu Fuel Form Activities has one main section: SRP-PuFF Facility. The SRL portion of this program has been completed. The program status, budget information, and milestone schedules are discussed. The SRP portion of this report summarizes production of STYPuO2 fuel forms for use in radioisotopic thermoelectric generators (RTG's) in the Plutonium Fuel Form (Puff) Facility at the Savannah River Plant. The PuFF Facility has been placed in a production readiness mode of operation pending funding of additional heat source programs.

  10. Active superconducting devices formed of thin films

    DOEpatents

    Martens, Jon S.; Beyer, James B.; Nordman, James E.; Hohenwarter, Gert K. G.

    1991-05-28

    Active superconducting devices are formed of thin films of superconductor which include a main conduction channel which has an active weak link region. The weak link region is composed of an array of links of thin film superconductor spaced from one another by voids and selected in size and thickness such that magnetic flux can propagate across the weak link region when it is superconducting. Magnetic flux applied to the weak link region will propagate across the array of links causing localized loss of superconductivity in the links and changing the effective resistance across the links. The magnetic flux can be applied from a control line formed of a superconducting film deposited coplanar with the main conduction channel and weak link region on a substrate. The devices can be formed of any type to superconductor but are particularly well suited to the high temperature superconductors since the devices can be entirely formed from coplanar films with no overlying regions. The devices can be utilized for a variety of electrical components, including switching circuits, amplifiers, oscillators and modulators, and are well suited to microwave frequency applications.

  11. Four olive branches: What the FERC bestowed on PUCs in Order 888

    SciTech Connect

    Hanger, J.R.

    1996-10-01

    Where others see conflict, a Pennsylvania commissioner finds a peace offering, not a grab for power. The jurisdictional issues posed by Order 888 continue to breed tension between federal and state officials. Unfortunately, most of this tension too often elevates form over substance. This jurisdictional tension shifts the focus of decisionmaking from securing the benefits of competition to preserving regulatory turf. In Order 888, the Federal Energy Regulatory Commission (FERC) extends four important olive branches to those states that want to introduce customer choice at the retail level. While these olive branches are not a fundamental concession by the FERC, state officials should pursue them before attacking Order 888 for its broad claims over jurisdiction.

  12. FERC approves R and D budget for Gas Research Institute

    SciTech Connect

    Not Available

    1990-11-08

    The Federal Energy Regulatory Commission (FERC) has approved the Gas Research Institute (GRI) five-year research and development plan for 1991 to 1995. The program provides funding for 231 projects in three primary R and D areas including end use, gas operations, and supply. Natural gas deliverability is a major focus of the 1991 to 1995 R and D plan, according to GRI. In approving the budget, the FERC rejected arguments made by large industrial users that GRI's funding unit was too high; that its programs are not well-conceived; and, that its end-use programs will not result in benefits to ratepayers within a reasonable period of time. The large industrial users presented no new arguments and merely incorporate the facts and arguments already addressed, FERC concluded.

  13. 76 FR 5799 - Notice of Commissioners and Staff Attendance at FERC Leadership Development Program Induction...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-02

    ... Energy Regulatory Commission Notice of Commissioners and Staff Attendance at FERC Leadership Development...: FERC Leadership Development Program Induction Ceremony: 888 First Street, NE., Washington, DC 20426... Leadership Development Program. Kimberly D. Bose, Secretary. BILLING CODE 6717-01-P...

  14. 76 FR 42129 - Agency Information Collection Activities: Case Submission Form, Case Assistance Form

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-18

    ... SECURITY Agency Information Collection Activities: Case Submission Form, Case Assistance Form (Form DHS-7001), Online Ombudsman Form DHS-7001 AGENCY: Office of the Citizenship and Immigration Service... practices of USCIS to mitigate problems. This form is used by an applicant who is experiencing problems...

  15. Implications of the Bangor Hydro decision on FERC relicensings

    USGS Publications Warehouse

    Pizzi, L.

    1997-01-01

    In the next 15 years, over 400 existing nonfederal dams will require new licenses by the Federal Energy Regulatory Commission (FERC) in order to continue operations. In many cases, state or federal agencies have a unique opportunity to condition the hydropower licenses to protect environmental values. In 1996, the Court of Appeals for the District of Columbia Circuit issued an opinion striking down a mandatory license condition imposed by the U.S. Department of the Interior in a FERC relicensing. The case, Bangor Hydro-Electric Company v.Federal Energy Regulatory Commission, is instructive for agencies regarding the procedures to be followed in imposing mandatory FERC licensing conditions in the future. After Bangor Hydro, agencies should: (1) support their decisions by substantial evidence in the FERC record, (2) request applicant studies to support agency decisions and allow public comment on the decisions as a means to test the facts and analysis, and (3) consider intervening in a case to defend the condition on appeal. Bangor Hydro also raises, but does not decide, the issue of whether the agency imposing the mandatory condition should engage in balancing the economics of the hydropower project with the public resources affected by the project.

  16. 75 FR 49914 - Commission Information Collection Activities (FERC-511 and FERC-515); Comment Request; Extensions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-16

    ... receipt of eFiled comments. Commenters making an eFiling should not make a paper filing. Commenters that are not able to file electronically must send an original and two (2) paper copies of their comments...Filed) or in paper format, and should refer to Docket Nos. IC10-511-000 and IC10- 515-000. (For...

  17. 78 FR 69843 - Commission Information Collection Activities (Ferc-Ferc-549d); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-21

    ... Transportation and Storage Report for Intrastate Natural Gas and Hinshaw Pipelines). DATES: Comments on the... fax at (202) 273-0873. SUPPLEMENTARY INFORMATION: Title: Quarterly Transportation and Storage Report...--Quarterly Transportation and Storage Report for Intrastate Natural Gas and Hinshaw Pipelines Number...

  18. 75 FR 35001 - Commission Information Collection Activities (FERC-FERC-725E); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... operators, balancing authorities and load-serving entities to document and report to WECC actions taken in...-725E Reporting: Balancing Authorities 32 1 20 640 Generator Operators 196 1 10 1960 Load-Serving... Balancing Authorities 64 Generator Operators 196 Load-Serving Entities 140 Transmission Owners/Operators...

  19. Neural network with formed dynamics of activity

    SciTech Connect

    Dunin-Barkovskii, V.L.; Osovets, N.B.

    1995-03-01

    The problem of developing a neural network with a given pattern of the state sequence is considered. A neural network structure and an algorithm, of forming its bond matrix which lead to an approximate but robust solution of the problem are proposed and discussed. Limiting characteristics of the serviceability of the proposed structure are studied. Various methods of visualizing dynamic processes in a neural network are compared. Possible applications of the results obtained for interpretation of neurophysiological data and in neuroinformatics systems are discussed.

  20. Silk Microgels Formed by Proteolytic Enzyme Activity

    PubMed Central

    Samal, Sangram K.; Dash, Mamoni; Chiellini, Federica; Kaplan, David L.; Chiellini, Emo

    2013-01-01

    The proteolytic enzyme α-chymotrypsin selectively cleaves the amorphous regions of silk fibroin protein (SFP) and allows the crystalline regions to self-assemble into silk microgels (SMG) at physiological temperature. These microgels consist of lamellar crystals in the micrometer scale, in contrast to the nanometer scaled crystals in native silkworm fibers. SDS-PAGE and zeta potential results demonstrated that α-chymotrypsin utilized only the nonamorphous domains or segments of the heavy chain of SFP to form negatively charged SMGs. The SMGs were characterized in terms of size, charge, structure, morphology, crystallinity, swelling kinetics, water content and thermal properties. The results suggest that the present technique of preparing SMGs by α-chymotrypsin is simple and efficient potential and that the prepared SMGS have useful features for studies related to biomaterials and pharmaceutical needs. This process is also an easy approach to obtain the amorphous peptide chains for further study. PMID:23756227

  1. Silk microgels formed by proteolytic enzyme activity.

    PubMed

    Samal, Sangram K; Dash, Mamoni; Chiellini, Federica; Kaplan, David L; Chiellini, Emo

    2013-09-01

    The proteolytic enzyme α-chymotrypsin selectively cleaves the amorphous regions of silk fibroin protein (SFP) and allows the crystalline regions to self-assemble into silk microgels (SMGs) at physiological temperature. These microgels consist of lamellar crystals in the micrometer scale, in contrast to the nanometer-scaled crystals in native silkworm fibers. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zeta potential results demonstrated that α-chymotrypsin utilized only the non-amorphous domains or segments of the heavy chain of SFP to form negatively charged SMGs. The SMGs were characterized in terms of size, charge, structure, morphology, crystallinity, swelling kinetics, water content and thermal properties. The results suggest that the present technique of preparing SMGs by α-chymotrypsin is simple and efficient, and that the prepared SMGs have useful features for studies related to biomaterial and pharmaceutical needs. This process is also an easy way to obtain the amorphous peptide chains for further study. PMID:23756227

  2. 76 FR 61725 - Agency Information Collection Activities: Case Submission Form, Case Assistance Form; (Form DHS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... Federal Register on July 18, 2011 at 76 FR 42129, for a 60-day public comment period. No comments were... other forms of information technology, e.g., permitting electronic submissions of responses. FOR FURTHER... to bring to the attention of the CIS Ombudsman (``trend''). For case problems, the CIS Ombudsman...

  3. Pu-238 fuel form activities, January 1-31, 1982

    SciTech Connect

    Not Available

    1982-03-01

    This monthly report for /sup 238/Pu fuel form activities has two main sections: SRP-PuFF facility and SRL fuel form activities. The program status, budget information, and milestone schedules are discussed in each main section. The Work Breakdown Structure (WBS) for this program is shown. Only one monthly report per year is processed for EDB.

  4. Pu-238 fuel form activities, January 1-31, 1981

    SciTech Connect

    Not Available

    1981-02-01

    This monthly report for /sup 238/Pu Fuel Form Activities has two main sections: SRP-PuFF facility and SRL Fuel Form Activities. The program status, budget information, and milestone schedules are discussed in each main section. The Work Breakdown Structure (WBS) for this program is shown. Only one monthly report per year is processed for EDB.

  5. Pu-238 fuel form activities, January 1-31, 1983

    SciTech Connect

    Not Available

    1983-03-01

    This monthly report for /sup 238/Pu Fuel Form Activities has two main sections: SRP-PuFF facility and SRL Fuel Form Activities. The program status, budget information, and milestone schedules are discussed in each main section. The Work Breakdown Structure (WBS) for this program is shown. Only one monthly report per year is processed for EDB.

  6. With stroke of pen, FERC puts DR on par with supply side options

    SciTech Connect

    2009-01-15

    In mid October 2008, the Federal Energy Regulatory Commission (FERC) finalized new rules intended to strengthen the operation and improve the competitiveness of organized U.S. wholesale electric markets. FERC intends to increase the use of demand response (DR), encourage long-term power contracts, strengthen the role of market monitors, and enhance the responsiveness of regional transmission organizations (RTOs) and independent system operators (ISOs). The FERC order applies to existing U.S. organized wholesale markets.

  7. 76 FR 46668 - Revision to Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ...\\ Five-Year Review of Oil Pipeline Pricing Index, 75 FR 80300, 80308 (Dec. 22, 2010), 133 FERC ] 61,228... Policy Act, Order No. 486, 486 FR 1750 (Jan. 22, 1988), FERC Stats. & Regs. ] 30,783 (1987). \\18\\ 18 CFR... Energy Regulatory Commission 18 CFR Part 357 Revision to Form No. 6 AGENCY: Federal Energy...

  8. Pu-238 fuel form activities, June 1-30, 1980

    SciTech Connect

    Not Available

    1980-07-18

    This monthly report for Pu-238 Fuel Form Activities has two main sections: SRP-PuFF Pu-238 Fuel Form Production Processes and SRL Pu-238 Fuel Form Research and Development. The program status, budget information, and milestone information are discussed in each main section. The Work Breakdown Structures (WBS) for this program is outlined. Only one monthly report per year is processed for EDB.

  9. Form-Focused Discovery Activities in English Classes

    ERIC Educational Resources Information Center

    Ogeyik, Muhlise Cosgun

    2011-01-01

    Form-focused discovery activities allow language learners to grasp various aspects of a target language by contributing implicit knowledge by using discovered explicit knowledge. Moreover, such activities can assist learners to perceive and discover the features of their language input. In foreign language teaching environments, they can be used…

  10. 30 CFR 585.903 - What are the requirements for decommissioning FERC-licensed hydrokinetic facilities?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false What are the requirements for decommissioning FERC-licensed hydrokinetic facilities? 585.903 Section 585.903 Mineral Resources BUREAU OF OCEAN ENERGY... What are the requirements for decommissioning FERC-licensed hydrokinetic facilities? You must...

  11. 30 CFR 585.903 - What are the requirements for decommissioning FERC-licensed hydrokinetic facilities?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 2 2014-07-01 2014-07-01 false What are the requirements for decommissioning FERC-licensed hydrokinetic facilities? 585.903 Section 585.903 Mineral Resources BUREAU OF OCEAN ENERGY... What are the requirements for decommissioning FERC-licensed hydrokinetic facilities? You must...

  12. 30 CFR 285.903 - What are the requirements for decommissioning FERC-licensed hydrokinetic facilities?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 2 2011-07-01 2011-07-01 false What are the requirements for decommissioning FERC-licensed hydrokinetic facilities? 285.903 Section 285.903 Mineral Resources BUREAU OF OCEAN ENERGY... Requirements § 285.903 What are the requirements for decommissioning FERC-licensed hydrokinetic facilities?...

  13. 30 CFR 285.903 - What are the requirements for decommissioning FERC-licensed hydrokinetic facilities?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What are the requirements for decommissioning FERC-licensed hydrokinetic facilities? 285.903 Section 285.903 Mineral Resources MINERALS MANAGEMENT... the requirements for decommissioning FERC-licensed hydrokinetic facilities? You must comply with...

  14. 30 CFR 585.903 - What are the requirements for decommissioning FERC-licensed hydrokinetic facilities?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 2 2012-07-01 2012-07-01 false What are the requirements for decommissioning FERC-licensed hydrokinetic facilities? 585.903 Section 585.903 Mineral Resources BUREAU OF OCEAN ENERGY....903 What are the requirements for decommissioning FERC-licensed hydrokinetic facilities? You...

  15. The impact of FERC orders on the value of bidders in PJM

    SciTech Connect

    Peters, Lon L.

    2009-08-15

    A study of the responses of share prices of major bidders in the PJM auctions from the mid-1990s through 2006 finds statistically significant effects for some FERC decisions for a portfolio of bidders. In contrast to publicly stated policy objectives, the analysis shows that some FERC decisions significantly raised market expectations regarding the future profitability of bidders. (author)

  16. Activated carbon fibers and engineered forms from renewable resources

    DOEpatents

    Baker, Frederick S.

    2010-06-01

    A method of producing activated carbon fibers (ACFs) includes the steps of providing a natural carbonaceous precursor fiber material, blending the carbonaceous precursor material with a chemical activation agent to form chemical agent-impregnated precursor fibers, spinning the chemical agent-impregnated precursor material into fibers, and thermally treating the chemical agent-impregnated precursor fibers. The carbonaceous precursor material is both carbonized and activated to form ACFs in a single step. The method produces ACFs exclusive of a step to isolate an intermediate carbon fiber.

  17. Activated carbon fibers and engineered forms from renewable resources

    DOEpatents

    Baker, Frederick S

    2013-02-19

    A method of producing activated carbon fibers (ACFs) includes the steps of providing a natural carbonaceous precursor fiber material, blending the carbonaceous precursor material with a chemical activation agent to form chemical agent-impregnated precursor fibers, spinning the chemical agent-impregnated precursor material into fibers, and thermally treating the chemical agent-impregnated precursor fibers. The carbonaceous precursor material is both carbonized and activated to form ACFs in a single step. The method produces ACFs exclusive of a step to isolate an intermediate carbon fiber.

  18. Supreme court agrees: FERC must regulate wholesale markets

    SciTech Connect

    Wolak, Frank A.

    2008-08-15

    The author believes that wholesale markets in the United States would have a greater likelihood of ultimately benefiting consumers if the Federal Energy Regulatory Commission did not have the mandate under the Federal Power Act (FPA) to ensure that wholesale prices are ''just and reasonable.'' However, he continues to believe that the FERC cannot avoid having an ex post criteria for asssessing whether market prices are just and reasonable. Moreover, changes in the design and regulatory oversight of U.S. wholesale electricity markets in recent years, including the recent Supreme Court decision, have caused him to believe even more strongly in the guardrails-for-market-outcomes approach. Finally, several questions are addressed which relate to the pricing of fixed-price, long-term contracts and the impact of these obligations on the behavior of suppliers in short-term wholesale markets that are directly relevant to answering the two major questions that the Supreme Court remanded to FERC in its recent decision.

  19. 75 FR 35700 - Revisions to Forms, Statements, and Reporting Requirements for Natural Gas Pipelines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... for Natural Gas Pipelines June 17, 2010. AGENCY: Federal Energy Regulatory Commission, DOE. ACTION... Natural Gas Pipelines, Order No. 710, FERC Stats. & Regs. ] 31,267 (2008), order on reh' g and... revised its financial forms, statements, and reports for natural gas companies, contained in FERC Form...

  20. 75 FR 51093 - Agency Information Collection Activities: Form I-864, Form I-864A, Form I-864EZ, and Form I-864W...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... on May 12, 2010, at 75 FR 26782, allowing for a 60-day public comment period. USCIS received 2... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-864.... Citizenship and Immigration Services (USCIS) will be submitting the following information collection...

  1. 75 FR 26782 - Agency Information Collection Activities: Form I-864, Form I-864A, Form I-864EZ, and Form I-864W...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-864... Exemption; OMB Control No. 1615-0075. The Department of Homeland Security, U.S. Citizenship and Immigration...-864W; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  2. Multiple forms of acid phosphatase activity in Gaucher's disease.

    PubMed

    Chambers, J P; Peters, S P; Glew, R H; Lee, R E; McCafferty, L R; Mercer, D W; Wenger, D A

    1978-07-01

    Although the primary genetic defect in all individuals with Gaucher's disease is a deficiency in glucocerebrosidase activity, the finding of marked elevations in splenic and serum acid phosphatase activity is almost as consistent a finding. Gaucher spleen and serum contain at least two forms of acid phosphatase that can be readily separated by chromatography on columns containing the cation exchange resin Sulphopropyl Sephadex. The major species of acid phosphatase (designated SP-I) contained in Triton X-100 (1% v/v) extracts of Gaucher spleen accounts for 65%--95% of the total activity and has the following properties: (1) it does not bind to the cation exchange column; (2) it exhibitis a pH optimum of 4.5--5.0; (3) it is inhibited by sodium fluoride (15 mM), L(+)-tartaric acid (20 mM), and beta-mercaptoethanol (2.1 M), and (4) it is resistant to inhibition by sodium dithionite (10 mM). The minor acid phosphatase activity (designated SP-II) present in extracts of Gaucher spleen has properties similar to those of the major species of acid phosphatase activity contained in serum from patients with Gaucher's disease: (1) it binds firmly to cation exchange columns (eluted by 0.5 M sodium chloride); (2) it exhibits a pH optimum of 5.0--6.0; (3) it is inhibited by sodium fluoride and sodium dithionite; and (4) it is resistant to inhibition by beta-mercaptoethanol (2.1 M) and L(+)-tartaric acid (20 mM). In addition, a second form of acid phosphatase that is tartrate resistant was found to be elevated in Gaucher serum. This form of serum acid phosphatase did not bind to Sulphopropyl Sephadex, was found to be significantly resistant to beta-mercaptoethanol (2.1 M), and was only partially inhibited by sodium dithionite (10 mM). The findings reported here indicate that at least three distinct forms of acid phosphatase activity are elevated in Gaucher's disease. Furthermore, the minor acid phosphatase activity contained in spleen homogenates has properties very similar to

  3. 76 FR 65191 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-20

    ..., ER11-4580-000] California Independent System Operator Corporation; Notice of FERC Staff Attendance The... Independent System Operator (CAISO). ] The agenda and other documents for the teleconferences and meetings...

  4. 77 FR 41402 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-13

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission California Independent System Operator Corporation; Notice of FERC... California Independent System Operator (CAISO). The agenda and other documents for the teleconferences...

  5. 76 FR 76157 - California Independent System, Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-06

    ...; ER11-4580-000; ER12-50-000] California Independent System, Operator Corporation; Notice of FERC Staff... California Independent System Operator (CAISO). The agenda and other documents for the teleconferences...

  6. 78 FR 34093 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission California Independent System Operator Corporation; Notice of FERC Staff... California Independent System Operator (CAISO). The agenda and other documents for the teleconferences...

  7. Policymaking and politics in an independent commission: Environmental policy in the FERC hydroelectric licensing program

    NASA Astrophysics Data System (ADS)

    Spence, David B.

    This work addresses the question of how effectively elected politicians can exert influence over the policy choices of regulatory agencies. I use environmental policy decisions made by the Federal Energy Regulatory Commission ("FERC") in its hydroelectric licensing program to test several competing hypotheses from the political control literature. I find, among other things, that: (1) politicians have used the tools of political control to effect noticeable but marginal changes in the content of FERC decisions over time; (2) the FERC has used its retained decision-making and procedural discretion strategically to minimize the both the effects of political controls and the influence of environmental group intervenors over agency decisions; and (3) FERC decisions remained relatively consistent over time in the face of repeated attempts by others to pull it toward a more pro-environment position.

  8. Channel-Forming Activities in the Glycosomal Fraction from the Bloodstream Form of Trypanosoma brucei

    PubMed Central

    Miinalainen, Ilkka J.; Hiltunen, J. Kalervo; Michels, Paul A. M.; Antonenkov, Vasily D.

    2012-01-01

    Background Glycosomes are a specialized form of peroxisomes (microbodies) present in unicellular eukaryotes that belong to the Kinetoplastea order, such as Trypanosoma and Leishmania species, parasitic protists causing severe diseases of livestock and humans in subtropical and tropical countries. The organelles harbour most enzymes of the glycolytic pathway that is responsible for substrate-level ATP production in the cell. Glycolysis is essential for bloodstream-form Trypanosoma brucei and enzymes comprising this pathway have been validated as drug targets. Glycosomes are surrounded by a single membrane. How glycolytic metabolites are transported across the glycosomal membrane is unclear. Methods/Principal Findings We hypothesized that glycosomal membrane, similarly to membranes of yeast and mammalian peroxisomes, contains channel-forming proteins involved in the selective transfer of metabolites. To verify this prediction, we isolated a glycosomal fraction from bloodstream-form T.brucei and reconstituted solubilized membrane proteins into planar lipid bilayers. The electrophysiological characteristics of the channels were studied using multiple channel recording and single channel analysis. Three main channel-forming activities were detected with current amplitudes 70–80 pA, 20–25 pA, and 8–11 pA, respectively (holding potential +10 mV and 3.0 M KCl as an electrolyte). All channels were in fully open state in a range of voltages ±150 mV and showed no sub-conductance transitions. The channel with current amplitude 20–25 pA is anion-selective (PK+/PCl−∼0.31), while the other two types of channels are slightly selective for cations (PK+/PCl− ratios ∼1.15 and ∼1.27 for the high- and low-conductance channels, respectively). The anion-selective channel showed an intrinsic current rectification that may suggest a functional asymmetry of the channel's pore. Conclusions/Significance These results indicate that the membrane of glycosomes apparently

  9. NUCLEAR ACTIVITY IS MORE PREVALENT IN STAR-FORMING GALAXIES

    SciTech Connect

    Rosario, D. J.; Lutz, D.; Berta, S.; Popesso, P.; Genzel, R.; Saintonge, A.; Tacconi, L.; Wuyts, S. E-mail: lutz@mpe.mpg.de E-mail: popesso@mpe.mpg.de E-mail: amelie@mpe.mpg.de E-mail: swuyts@mpe.mpg.de; and others

    2013-07-01

    We explore the question of whether low and moderate luminosity active galactic nuclei (AGNs) are preferentially found in galaxies that are undergoing a transition from active star formation (SF) to quiescence. This notion has been suggested by studies of the UV-optical colors of AGN hosts, which find them to be common among galaxies in the so-called Green Valley, a region of galaxy color space believed to be composed mostly of galaxies undergoing SF quenching. Combining the deepest current X-ray and Herschel/PACS far-infrared (FIR) observations of the two Chandra Deep Fields with redshifts, stellar masses, and rest-frame photometry derived from the extensive and uniform multi-wavelength data in these fields, we compare the rest-frame U - V color distributions and star formation rate distributions of AGNs and carefully constructed samples of inactive control galaxies. The UV-to-optical colors of AGNs are consistent with equally massive inactive galaxies at redshifts out to z {approx} 2, but we show that such colors are poor tracers of SF. While the FIR distributions of both star-forming AGNs and star-forming inactive galaxies are statistically similar, we show that AGNs are preferentially found in star-forming host galaxies, or, in other words, AGNs are less likely to be found in weakly star-forming or quenched galaxies. We postulate that, among X-ray-selected AGNs of low and moderate accretion luminosities, the supply of cold gas primarily determines the accretion rate distribution of the nuclear black holes.

  10. Active curcumin nanoparticles formed from a volatile microemulsion template.

    PubMed

    Margulis, K; Srinivasan, S; Ware, M J; Summers, H D; Godin, B; Magdassi, S

    2014-01-01

    We report on biological performance of organic nanoparticles formed by a simple method based on rapid solvent removal from a volatile microemulsion. The particular focus of the study was on testing the suitability of the method for substances soluble in partially water-miscible organic solvents as well as on evaluating the therapeutic activity of the resultant nanoparticles. Curcumin was employed as a model for hydrophobic drug, and, as it is soluble in water-miscible organic solvents, it was successfully incorporated into a new cyclopentanone-water microemulsion system. During rapid solvent removal by spray-drying, the nanometric droplets of the microemulsion were converted into nanoparticles containing amorphous curcumin with the average size of 20.2±3.4 nm, having ζ potential of -36.2 ±1.8 mV. These nanoparticles were dispersible in water and retained the high loading of the active substance. The therapeutic activity of the resulting nanoparticles was demonstrated in a pancreatic cancer cell line Panc-1. The effective concentration for reducing the metabolic activity was found to be 11.5 μM for nanoparticles compared with 19.5 μM for free curcumin. PMID:25485110

  11. 76 FR 27077 - Agency Information Collection Activities: Form AR-11 and Form AR-11SR, Extension of an Existing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-10

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form AR-11 and...; OMB Control No. 1615-0007. The Department of Homeland Security, U.S. Citizenship and Immigration... collection: Form AR-11 and Form AR-11SR. U.S. Citizenship and Immigration Services. (4) Affected public...

  12. 75 FR 16492 - Agency Information Collection Activities: Form G-28, and Form G-28I, Revision of an Existing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-28, and... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) has submitted the... sponsoring the collection: Form G-28, and Form G-28I. U.S. Citizenship and Immigration Services (USCIS)....

  13. Research report: Summary of key state issues of FERC orders 888 and 889

    SciTech Connect

    Rose, K.; Burns, R.E.; Graniere, R.J.

    1997-01-01

    The Federal Energy Regulatory Commission`s (FERC) Order 888 is perhaps the most important and far reaching FERC electricity order in decades. The consequences on the structure of the industry and how the industry is regulated are significant departures from past methods and regulatory philosophy. This will undoubtedly have a dramatic impact on the manner in which state public utility commissions, which are also undergoing or considering dramatic change, regulate their jurisdictional electric utilities. This report summarizes and discusses the actions that the FERC is taking and their profound repercussions on the industry and state commissions. The report is not a comprehensive summary of the entire order. Rather, it is intended to highlight the order`s more important features and discuss what this could mean for the states. The report is organized into eight sections; the first seven address Order 888 and the last section (section 8) addresses Order 889. Section 1 through 5 summarize and discuss the main features of Order 888. Section 6 (on jurisdiction) and Section 7 (on property rights) interpret the likely consequences of the order. Section 8, summarizes the FERC`s Open Access Same Time System (OASIS) and discusses some concerns about its real-world application.

  14. Pu-238 fuel form activities, January 1-31, 1985

    SciTech Connect

    Not Available

    1985-07-01

    This monthly report summarizes production of /sup 238/PuO/sub 2/ fuel forms for use in radioisotopic thermoelectric generators (RTG's) in the Plutonium Fuel Form (PuFF) Facility at the Savannah River Plant.

  15. Tectonic activity on Pluto after the Charon-forming impact

    NASA Astrophysics Data System (ADS)

    Barr, Amy C.; Collins, Geoffrey C.

    2015-01-01

    The Pluto-Charon system, likely formed from an impact, has reached the endpoint of its tidal evolution. During its evolution into the dual-synchronous state, the equilibrium tidal figures of Pluto and Charon would have also evolved as angular momentum was transferred from Pluto's spin to Charon's orbit. The rate of tidal evolution is controlled by Pluto's interior physical and thermal state. We examine three interior models for Pluto: an undifferentiated rock/ice mixture, differentiated with ice above rock, and differentiated with an ocean. For the undifferentiated case without an ocean, the Pluto-Charon binary does not evolve to its current state unless its internal temperature Ti > 200K , which would likely lead to strong tidal heating, melting, and differentiation. Without an ocean, Pluto's interior temperature must be higher than 240 K for Charon to evolve on a time scale less than the age of the Solar System. Further tidal heating would likely create an ocean. If New Horizons finds evidence of ancient tidally-driven tectonic activity on either body, the most likely explanation is that Pluto had an internal ocean during Charon's orbital evolution.

  16. 76 FR 30738 - Agency Information Collection Activities: Form G-845 and Form G-845 Supplement, Revision of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-26

    ... the Federal Register on February 22, 2011, at 76 FR 9805, allowing for a 60-day public comment period... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-845 and Form G- 845 Supplement, Revision of a Currently Approved Information Collection; Comment Request...

  17. The Essay: Theory and Pedagogy for an Active Form.

    ERIC Educational Resources Information Center

    Heilker, Paul

    Calling for a radical reexamination of the traditional foundation of composition instruction--the thesis/support form, this book argues that the essay, with its informality, conversational tone, meditative mood, and integration of form and content, is better suited to developmental, epistemological, ideological, and feminist rhetorical…

  18. Structure and Form. Elementary Science Activity Series, Volume 2.

    ERIC Educational Resources Information Center

    Blackwell, Frank F.

    This book is number 2 of a series of elementary science books that presents a wealth of ideas for science activities for the elementary school teacher. Each activity includes a standard set of information designed to help teachers determine the activity's appropriateness for their students, plan its implementation, and help children focus on a…

  19. 77 FR 26537 - Notice of Commissioners and Staff Attendance at FERC Leadership Development Program Graduation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-04

    ... Energy Regulatory Commission Notice of Commissioners and Staff Attendance at FERC Leadership Development... Leadership Development Program Graduation/Induction Ceremony: 888 First Street NE., Washington, DC 20426. May... 2012 Leadership Development Program and graduate 15 employees from the 2011 program. Dated: April...

  20. 77 FR 29634 - Notice of FERC Staff Attendance at the Louisiana Public Service Commission's Business and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... Energy Regulatory Commission Notice of FERC Staff Attendance at the Louisiana Public Service Commission's... members of its staff may attend the meeting noted below. Their attendance is part of the Commission's... Council Auditorium, 705 West University Ave., Lafayette, LA 70506. The discussions may address matters...

  1. 76 FR 29235 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ...] California Independent System Operator Corporation; Notice of FERC Staff Attendance The Federal Energy... participate in teleconferences and meetings to be conducted by the California Independent System Operator... Web site, http://www.caiso.com . May 17, 2011, Systems Interface User Group, Department of...

  2. 76 FR 55380 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ...; ER11-4384-000] California Independent System Operator Corporation; Notice of FERC Staff Attendance The... Independent System Operator (CAISO). The agenda and other documents for the teleconferences and meetings are..., Outage Management System Business Requirements. September 15, 2011 Fall 2011 Release Market...

  3. 77 FR 63811 - Notice of Commissioners and Staff Attendance at FERC Author Speaker Series Event

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ... Energy Regulatory Commission Notice of Commissioners and Staff Attendance at FERC Author Speaker Series... the Commission and/or Commission staff may attend the following event: Author Speaker Series featuring... event will feature Pulitzer Prize winning author, Daniel Yergin, presenting on his most recent book,...

  4. 77 FR 66609 - Notice of FERC Staff Attendance; PacifiCorp, et al.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ... Public Service Company......... ER13-82-000 El Paso Electric Company ER13-91-000 Black Hills Power, Inc., et al......... ER13-96-000 Black Hills Colorado Electric Utility ER13-97-000 Company. NV Energy, Inc...@ferc.gov . Dated: October 31, 2012. Kimberly D. Bose, Secretary. BILLING CODE 6717-01-P...

  5. 76 FR 13699 - Reports, Forms and Recordkeeping Requirements Information Collection Activity Under OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ..., companies chartering ships from MARAD, and companies having Title XI guarantee obligations. Form(s): MA-172... TRANSPORTATION Maritime Administration Reports, Forms and Recordkeeping Requirements Information Collection Activity Under OMB Review AGENCY: Maritime Administration, DOT. ACTION: Notice and request for...

  6. A cytosolic activator of DNA replication is tyrosine phosphorylated in its active form.

    PubMed

    Fresa, K L; Autieri, M V; Coffman, F D; Georgoff, I; Cohen, S

    1993-04-01

    Cytosolic extracts from actively dividing lymphoid cells have been shown to induce DNA synthesis in isolated, quiescent nuclei. An initiating factor in such extracts (activator of DNA replication; ADR) is a > 90-kDa aprotinin-binding protein whose activity is inhibitable not only by aprotinin, but also by several other protease inhibitors as well. Although cytosol from non-proliferating lymphocytes is devoid of ADR activity, we have shown that these preparations can be induced to express ADR activity by brief exposure to a membrane-enriched fraction of spontaneously proliferating MOLT-4 cells via a kinase-dependent mechanism. In the present study, we examine the role of tyrosine kinases in this process. Three inhibitors of tyrosine kinases (genistein, kaempferol, and quercetin) can inhibit the in vitro generation of ADR activity. In vitro generation of ADR activity is associated with the de novo phosphorylation of several proteins, many of which are detectable using anti-phosphotyrosine monoclonal antibodies. ADR itself may be tyrosine phosphorylated in active form as immunoprecipitation using such monoclonal antibodies leads to the depletion of its activity. Moreover, immunoprecipitation results in the removal of several de novo tyrosine-phosphorylated proteins, including species at approximately 122, 105, 93, 86, 79, and 65 kDa. A subset of de novo-phosphorylated proteins, migrating at approximately 105, 93, and 70 kDa, also bound to aprotinin, suggesting that at least one of these proteins may represent ADR itself. PMID:7683270

  7. STYPu fuel form activities, March 1-September 30, 1985

    SciTech Connect

    Not Available

    1986-01-01

    The SRP portion of this report summarizes production STYPuO2 fuel forms for use in radioisotopic thermoelectric generators (RTG's) in the Plutonium Fuel Form (PuFF) Facility at the Savannah River Plant. The PuFF Facility began producing iridium-encapsulated, 62.5-watt STYPuO2 right circular cylinders for GPHS (General Purpose Heat Source) RTG's in June 1980; this program was completed in December 1983. The PuFF Facility has been placed in a production readiness mode of operation pending funding of additional heat source programs.

  8. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Pipeline Data for Depreciation Analysis. 357.3 Section 357.3 Conservation of Power and Water Resources... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  9. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Pipeline Data for Depreciation Analysis. 357.3 Section 357.3 Conservation of Power and Water Resources... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  10. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Pipeline Data for Depreciation Analysis. 357.3 Section 357.3 Conservation of Power and Water Resources... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  11. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Pipeline Data for Depreciation Analysis. 357.3 Section 357.3 Conservation of Power and Water Resources... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  12. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Pipeline Data for Depreciation Analysis. 357.3 Section 357.3 Conservation of Power and Water Resources... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  13. 77 FR 59348 - Revisions to Page 700 of FERC Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-27

    ... (line 1), Depreciation Expense (line 2), AFUDC Depreciation (line 3), Amortization of Deferred Earnings... Rate Base Write-Up (SRB),\\11\\ and (3) Accumulated Net Deferred Earnings.\\12\\ \\10\\ See Williams Pipeline... Deferred Earnings consists of the inflation component, which is deferred to be recovered in...

  14. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable. SPECIFIC... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from...

  15. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable. SPECIFIC... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from...

  16. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable. SPECIFIC... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from...

  17. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable. SPECIFIC... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from...

  18. Chromophore structure of the physiologically active form (Pfr) of phytochrome

    PubMed Central

    Rüdiger, W.; Thümmler, F.; Cmiel, E.; Schneider, S.

    1983-01-01

    Chromopeptides were prepared by proteolytic digestion of phytochrome (far-red absorbing form, Pfr) and of phycocyanin. The phycocyanobilin peptide, the chromophore of which is Z,Z,Z-configurated, was modified to the Z,Z,E isomeric chromophore. It has been demonstrated earlier that the Pfr chromopeptide and the Z,Z,E-configurated phycocyanin chromopeptide behave similarly with regard to spectral and chromatographic properties and reactivity. We present evidence here, obtained by high-resolution 1H NMR spectroscopy, that both the modified phycocyanobilin chromophore and the phytochrome chromophore obtained directly from Pfr are 15E-configurated. PMID:16593380

  19. Forming a Learning Culture to Promote Fracture Prevention Activities

    ERIC Educational Resources Information Center

    Hjalmarson, Helene V.; Strandmark, Margaretha

    2012-01-01

    Purpose: The purpose of this paper is to explore interprofessional experiences of incorporating fracture prevention activities in clinical practice inspired by an empowerment approach. Design/methodology/approach: Data collection consisted primarily of focus groups interviews, systematized and analyzed by the grounded theory method. The study took…

  20. Thrombomodulin Binding Selects the Catalytically Active Form of Thrombin.

    PubMed

    Handley, Lindsey D; Treuheit, Nicholas A; Venkatesh, Varun J; Komives, Elizabeth A

    2015-11-01

    Human α-thrombin is a serine protease with dual functions. Thrombin acts as a procoagulant, cleaving fibrinogen to make the fibrin clot, but when bound to thrombomodulin (TM), it acts as an anticoagulant, cleaving protein C. A minimal TM fragment consisting of the fourth, fifth, and most of the sixth EGF-like domain (TM456m) that has been prepared has much improved solubility, thrombin binding capacity, and anticoagulant activity versus those of previous TM456 constructs. In this work, we compare backbone amide exchange of human α-thrombin in three states: apo, D-Phe-Pro-Arg-chloromethylketone (PPACK)-bound, and TM456m-bound. Beyond causing a decreased level of amide exchange at their binding sites, TM and PPACK both cause a decreased level of amide exchange in other regions including the γ-loop and the adjacent N-terminus of the heavy chain. The decreased level of amide exchange in the N-terminus of the heavy chain is consistent with the historic model of activation of serine proteases, which involves insertion of this region into the β-barrel promoting the correct conformation of the catalytic residues. Contrary to crystal structures of thrombin, hydrogen-deuterium exchange mass spectrometry results suggest that the conformation of apo-thrombin does not yet have the N-terminus of the heavy chain properly inserted for optimal catalytic activity, and that binding of TM allosterically promotes the catalytically active conformation. PMID:26468766

  1. Characterizing Warm Molecular Hydrogen in Active Star-Forming Systems

    NASA Astrophysics Data System (ADS)

    Rangwala, Naseem

    2014-10-01

    Herschel observations of nearby star-forming galaxies have determined that the warm component of the molecular gas traced by the high-J CO lines dominates the luminosity (~90% of the total CO luminosity) and hence the energetics of the molecular ISM. At the temperatures (T = 300 - 2000 K) and densities (n_H < 1E6 per cubic cm) typically found in our survey, H2 emission is the dominant gas coolant, much more important than CO. A fundamental assumption of all analyses of CO emission has been that CO emission traces H2 over the entire range of physical conditions in the observed sources. However, a direct observational comparison of spatial distributions and kinematics of CO and H2 has never been made for the warm molecular gas. We propose to observe the warm H2, in S(1) and S(2) transitions, with the SOFIA-EXES instrument in a diverse sample of star-forming systems: NGC 253 (starburst nucleus), NGC 6240 (luminous infrared galaxy), NGC 1068 (Seyfert-2), and SgrB2(M)/(N) (Galactic hot cores). The primary goal is to compare these measurements with the warm CO (J = 6-5 transition) observed with the Atacama Large Millimeter Array (ALMA) to investigate differences in the kinematics and spatial distributions (for the extended targets) of the two molecules and thereby confirm whether CO is a reliable tracer of H2 in the warm gas.

  2. 76 FR 31972 - Agency Information Collection Activities: Form I-508 and Form I-508F, Extension of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-508 and... and Immigration Services (USCIS), will be submitting the following information collection request for... Immigration Services. (4) Affected public who will be asked or required to respond, as well as a...

  3. Multiple forms of endopeptidase activity from jojoba seeds.

    PubMed

    Wolf, M J; Storey, R D

    1990-01-01

    The cotyledons of 27 day post-germination jojoba seedlings (Simmondsia chinensis) contained five distinct endopeptidase activities separable by DEAE Bio-Gel and CM-cellulose ion exchange chromatography. The endopeptidases were purified 108- to 266-fold and their individuality was confirmed by activity-specific assays in native acrylamide gels along with differences in their Mr and catalytic properties. The five endopeptidases, which showed activity on model substrates and protein, were named EP Ia, EP Ib, EP II, EP III and EP IV. EP Ia was a serine proteinase with a pH optimum of ca 8 and Mr of 58,000. EP Ib, II and III were discrete cysteine proteinases showing pH optima of ca 6.8, 6.0 and 5.4 and Mr of 41,000, 47,000 and 35,000 respectively. EP IV was an aspartic acid proteinase with a ca pH optimum of 3.5 and Mr of 33,000.

  4. Active Curved Polymers Form Vortex Patterns on Membranes

    NASA Astrophysics Data System (ADS)

    Denk, Jonas; Huber, Lorenz; Reithmann, Emanuel; Frey, Erwin

    2016-04-01

    Recent in vitro experiments with FtsZ polymers show self-organization into different dynamic patterns, including structures reminiscent of the bacterial Z ring. We model FtsZ polymers as active particles moving along chiral, circular paths by Brownian dynamics simulations and a Boltzmann approach. Our two conceptually different methods point to a generic phase behavior. At intermediate particle densities, we find self-organization into vortex structures including closed rings. Moreover, we show that the dynamics at the onset of pattern formation is described by a generalized complex Ginzburg-Landau equation.

  5. Active Curved Polymers Form Vortex Patterns on Membranes.

    PubMed

    Denk, Jonas; Huber, Lorenz; Reithmann, Emanuel; Frey, Erwin

    2016-04-29

    Recent in vitro experiments with FtsZ polymers show self-organization into different dynamic patterns, including structures reminiscent of the bacterial Z ring. We model FtsZ polymers as active particles moving along chiral, circular paths by Brownian dynamics simulations and a Boltzmann approach. Our two conceptually different methods point to a generic phase behavior. At intermediate particle densities, we find self-organization into vortex structures including closed rings. Moreover, we show that the dynamics at the onset of pattern formation is described by a generalized complex Ginzburg-Landau equation. PMID:27176542

  6. Active Curved Polymers Form Vortex Patterns on Membranes.

    PubMed

    Denk, Jonas; Huber, Lorenz; Reithmann, Emanuel; Frey, Erwin

    2016-04-29

    Recent in vitro experiments with FtsZ polymers show self-organization into different dynamic patterns, including structures reminiscent of the bacterial Z ring. We model FtsZ polymers as active particles moving along chiral, circular paths by Brownian dynamics simulations and a Boltzmann approach. Our two conceptually different methods point to a generic phase behavior. At intermediate particle densities, we find self-organization into vortex structures including closed rings. Moreover, we show that the dynamics at the onset of pattern formation is described by a generalized complex Ginzburg-Landau equation.

  7. 77 FR 15787 - Agency Information Collection Activities: Form I-131, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-16

    ... the Form/Collection: Application for Travel Document. (3) Agency Form Number, if any, and the... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-131... Collection Under Review: Form I- 131, Application for Travel Document. The Department of Homeland Security,...

  8. 76 FR 81517 - Agency Information Collection Activities: Form I-131, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-28

    ... the Form/Collection: Application for Travel Document. (3) Agency form number, if any, and the... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-131... Collection Under Review: Form I- 131, Application for Travel Document. The Department of Homeland Security,...

  9. 76 FR 10385 - Agency Information Collection Activities: Various Contract Related Forms That Will be Included in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... SECURITY Agency Information Collection Activities: Various Contract Related Forms That Will be Included in the Homeland Security Acquisition Regulation, DHS FORM 0700-01, DHS FORM 0700-02, DHS FORM 0700-03...: The Department of Homeland Security, Office of Chief Procurement Officer, Acquisition Policy...

  10. 75 FR 21013 - Agency Information Collection Activities: Form N-644; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-22

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-644... Collection Under Review; Form N- 644, Application for Posthumous Citizenship; OMB Control No. 1615-0059. The..., USCIS will be evaluating whether to revise the Form N-644. Should USCIS decide to revise Form N-644...

  11. 76 FR 21913 - Agency Information Collection Activities: Form N-644; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-19

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-644... Collection Under Review: Form N- 644, Application for Posthumous Citizenship; OMB Control No. 1615-0059. The..., USCIS will be evaluating whether to revise the Form N-644. Should USCIS decide to revise Form N-644...

  12. Heat of Hydration of Low Activity Cementitious Waste Forms

    SciTech Connect

    Nasol, D.

    2015-07-23

    During the curing of secondary waste grout, the hydraulic materials in the dry mix react exothermally with the water in the secondary low-activity waste (LAW). The heat released, called the heat of hydration, can be measured using a TAM Air Isothermal Calorimeter. By holding temperature constant in the instrument, the heat of hydration during the curing process can be determined. This will provide information that can be used in the design of a waste solidification facility. At the Savannah River National Laboratory (SRNL), the heat of hydration and other physical properties are being collected on grout prepared using three simulants of liquid secondary waste generated at the Hanford Site. From this study it was found that both the simulant and dry mix each had an effect on the heat of hydration. It was also concluded that the higher the cement content in the dry materials mix, the greater the heat of hydration during the curing of grout.

  13. Identification of biotransformation products of citalopram formed in activated sludge.

    PubMed

    Beretsou, Vasiliki G; Psoma, Aikaterini K; Gago-Ferrero, Pablo; Aalizadeh, Reza; Fenner, Kathrin; Thomaidis, Nikolaos S

    2016-10-15

    Citalopram (CTR) is a worldwide highly consumed antidepressant which has demonstrated incomplete removal by conventional wastewater treatment. Despite its global ubiquitous presence in different environmental compartments, little is known about its behaviour and transformation processes during wastewater treatment. The present study aims to expand the knowledge on fate and transformation of CTR during the biological treatment process. For this purpose, batch reactors were set up to assess biotic, abiotic and sorption losses of this compound. One of the main objectives of the study was the identification of the formed transformation products (TPs) by applying suspect and non-target strategies based on liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS). The complementary use of reversed phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) for the identification of polar TPs, and the application of in-house developed quantitative structure-retention relationship (QSRR) prediction models, in addition to the comprehensive evaluation of the obtained MS/MS spectra, provided valuable information to support identification. In total, fourteen TPs were detected and thirteen of them were tentatively identified. Four compounds were confirmed (N-desmethylCTR, CTR amide, CTR carboxylic acid and 3-oxo-CTR) through the purchase of the corresponding reference standard. Probable structures based on diagnostic evidence were proposed for the additional nine TPs. Eleven TPs are reported for the first time. A transformation pathway for the biotransformation of CTR was proposed. The presence of the identified TPs was assessed in real wastewater samples through retrospective analysis, resulting in the detection of five compounds. Finally, the potential ecotoxicological risk posed by CTR and its TPs to different trophic levels of aquatic organisms was evaluated by means of risk quotients. PMID:27459150

  14. 76 FR 66944 - Agency Information Collection Activities: Form I-914; Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-28

    ... Information Collection Under Review: Form I- 914 and Supplements A and B, Application for T Nonimmigrant... required to respond, as well as a brief abstract: Primary: Individuals or households. Form I- 914 permits... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form...

  15. 76 FR 24908 - Agency Information Collection Activities: Form G-639; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-03

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-639... Collection Under Review; Form G- 639, Freedom of Information/Privacy Act Request; OMB Control No. 1615- 0102.... During this 60 day period, USCIS will be evaluating whether to revise the Form G-639. Should USCIS...

  16. 76 FR 28444 - Agency Information Collection Activities: Form G-884, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-884... information collection under review: Form G- 884, Request for the Return of Original Documents; OMB Control No... July 18, 2011. During this 60-day period, USCIS will be evaluating whether to revise the Form...

  17. 75 FR 23785 - Agency Information Collection Activities: Form G-639; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-04

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-639... Collection Under Review; Form G- 639, Freedom of Information/Privacy Act Request; OMB Control No. 1615- 0102... this 60 day period, USCIS will be evaluating whether to revise the Form G-639. Should USCIS decide...

  18. 75 FR 5099 - Agency Information Collection Activities: Form N-648, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-648... information collection under review: Form N- 648, Medical Certification for Disability Exceptions. OMB Control... component of the Department of Homeland Security sponsoring the collection: Form N-648. U.S. Citizenship...

  19. 76 FR 38197 - Agency Information Collection Activities; Form N-600K, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-29

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities; Form N-600K... information collection under review: form N- 600K, application for citizenship and issuance of certificate... sponsoring the collection: Form N-600K, U.S. Citizenship and Immigration Services. (4) Affected public...

  20. 76 FR 59710 - Agency Information Collection Activities: Form N-600; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-27

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-600... Collection Under Review; Form N- 600, Application for Certificate of Citizenship; OMB Control No. 1615- 0057... the Department of Homeland Security sponsoring the collection: Form N-600; U.S. Citizenship...

  1. 75 FR 13776 - Agency Information Collection Activities: Form N-300; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-23

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-300... Collection Under Review; Form N- 300, Application to File Declaration of Intention; OMB Control No. 1615-0078... this 60-day period, USCIS will be evaluating whether to revise the Form N-300. Should USCIS decide...

  2. 75 FR 18871 - Agency Information Collection Activities: Form N-600K, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-600K... Information Collection under Review: Form N- 600K, Application for Citizenship and Issuance of Certificate...: Form N-600K, U.S. Citizenship and Immigration Services. (4) Affected public who will be asked...

  3. Composition and topology of activity cliff clusters formed by bioactive compounds.

    PubMed

    Stumpfe, Dagmar; Dimova, Dilyana; Bajorath, Jürgen

    2014-02-24

    The assessment of activity cliffs has thus far mostly focused on compound pairs, although the majority of activity cliffs are not formed in isolation but in a coordinated manner involving multiple active compounds and cliffs. However, the composition of coordinated activity cliff configurations and their topologies are unknown. Therefore, we have identified all activity cliff configurations formed by currently available bioactive compounds and analyzed them in network representations where activity cliff configurations occur as clusters. The composition, topology, frequency of occurrence, and target distribution of activity cliff clusters have been determined. A limited number of large cliff clusters with unique topologies were identified that were centers of activity cliff formation. These clusters originated from a small number of target sets. However, most clusters were of small to moderate size. Three basic topologies were sufficient to describe recurrent activity cliff cluster motifs/topologies. For example, frequently occurring clusters with star topology determined the scale-free character of the global activity cliff network and represented a characteristic activity cliff configuration. Large clusters with complex topology were often found to contain different combinations of basic topologies. Our study provides a first view of activity cliff configurations formed by currently available bioactive compounds and of the recurrent topologies of activity cliff clusters. Activity cliff clusters of defined topology can be selected, and from compounds forming the clusters, SAR information can be obtained. The SAR information of activity cliff clusters sharing a/one specific activity and topology can be compared.

  4. Assessing the "need to negotiate" in FERC licensing consultations: a study of two hydropower projects

    USGS Publications Warehouse

    Coughlan, Beth A. K.; Burkardt, Nina; Fulton, David

    1993-01-01

    We investigated the “need to negotiate” in a comparative case study of multi-agency negotiations in the FERC licensing process. Researchers interviewed participants in two cases involving environmental consultations and asked about parties' level of need to negotiate throughout the process. Participants identified a need to negotiate, and when this need was strongly felt, there was an increased opportunity for an agreement to be reached. An intense need to negotiate by all parties is not a prerequisite to successful agreements. When key participants have a strong need to negotiate, they can instigate negotiations and encourage the involvement of other parties.

  5. 75 FR 16895 - Reports, Forms and Record Keeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-02

    ... National Highway Traffic Safety Administration Reports, Forms and Record Keeping Requirements; Agency Information Collection Activity Under OMB Review AGENCY: National Highway Traffic Safety Administration.... Mary Versailles, Office of Rulemaking, National Highway Traffic Safety Administration, 1200 New...

  6. 77 FR 47162 - Reports, Forms and Recordkeeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-07

    ... National Highway Traffic Safety Administration Reports, Forms and Recordkeeping Requirements; Agency Information Collection Activity Under OMB Review AGENCY: National Highway Traffic Safety Administration, DOT...- 5378. SUPPLEMENTARY INFORMATION: National Highway Traffic Safety Administration Title:...

  7. 76 FR 41331 - Reports, Forms and Recordkeeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-13

    ... TRANSPORTATION National Highway Traffic Safety Administration Reports, Forms and Recordkeeping Requirements; Agency Information Collection Activity Under OMB Review AGENCY: National Highway Traffic Safety... and fax number is (202) 366-7002. SUPPLEMENTARY INFORMATION: National Highway Traffic...

  8. 77 FR 72321 - Agency Information Collection Activities: Proposed Collection; Comment Request; Supplemental Form...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... Food and Nutrition Service Agency Information Collection Activities: Proposed Collection; Comment Request; Supplemental Form for Collecting Taxpayer Identifying Numbers AGENCY: Food and Nutrition Service... Porter, Director, Office of Internal Controls, Audits and Investigations, Food and Nutrition Service,...

  9. 76 FR 52014 - Agency Information Collection Activities: Applicant Information Form (1-783)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF JUSTICE Federal Bureau of Investigation Agency Information Collection Activities: Applicant Information Form (1-783) ACTION: 30-Day Notice of Information Collection. The Department of Justice (DOJ), Federal...

  10. 78 FR 19726 - Agency Information Collection Activities: Visa Waiver Program Carrier Agreement (CBP Form I-775)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-02

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Visa Waiver Program... requirement concerning the Visa Waiver Program Carrier Agreement (CBP Form I-775). This request for comment is... information collection: Title: Visa Waiver Program Carrier Agreement. OMB Number: 1651-0110. Form Number:...

  11. 40 CFR 761.205 - Notification of PCB waste activity (EPA Form 7710-53).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... handler must renotify the Agency include, but are not limited to the following: the company changes... CFR PART 761” on the manifests, records, and reports which they shall prepare under this subpart... using EPA Form 7710-53 and those activities change, the facility must resubmit EPA Form 7710-53...

  12. 78 FR 52824 - Proposed Information Collection (Bowel and Bladder Care Billing Form) Activity: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-26

    ... AFFAIRS Proposed Information Collection (Bowel and Bladder Care Billing Form) Activity: Comment Request... evaluate the Bowel and Bladder Care Billing Form used by caregivers of eligible Veterans to document time spent providing services related specifically to bowel and bladder care. DATES: Written comments...

  13. 77 FR 23271 - Agency Information Collection Activities: Form I-881; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-881... (DHS), U.S. Citizenship and Immigration Services (USCIS) will be submitting the following information... component of the Department of Homeland Security sponsoring the collection: Form I-881; U.S. Citizenship...

  14. 75 FR 51096 - Agency Information Collection Activities: Form N-400; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-400... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the... of Homeland Security sponsoring the collection: Form N-400; U.S. Citizenship and Immigration...

  15. 77 FR 16047 - Agency Information Collection Activities: Form I-589; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-19

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-589.... 1615-0067. The Department Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be...: Form I-589; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  16. 75 FR 51094 - Agency Information Collection Activities: Form N-600; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-600... Collection under Review; Form N- 600, Application for Certificate of Citizenship; OMB Control No. 1615- 0057. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will...

  17. 75 FR 51095 - Agency Information Collection Activities: Form N-336; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-336... Section 336; OMB Control No. 1615-0050. The Department of Homeland Security, U.S. Citizenship and... sponsoring the collection: Form N-336; U.S. Citizenship and Immigration Services (USCIS). (4) Affected...

  18. 77 FR 36285 - Agency Information Collection Activities: Form I-693, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-693... Control No. 1615-0033. The Department of Homeland Security (DHS), U.S. Citizenship and Immigration... of the Department of Homeland Security sponsoring the collection: Form I-693. U.S. Citizenship...

  19. 76 FR 55081 - Agency Information Collection Activities: Form I-129S; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-06

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-129S... Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the following... collection: Form I-129S; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will...

  20. 76 FR 63322 - Agency Information Collection Activities: Form G-28, Revision of an Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-28.... The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be... Department of Homeland Security sponsoring the collection: Form G-28. U.S. Citizenship and...

  1. 75 FR 54646 - Agency Information Collection Activities: Form I-914; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-08

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-914.... Citizenship and Immigration Services (USCIS) will be submitting the following information collection request... of the Department of Homeland Security sponsoring the collection: Form I-914; U.S. Citizenship...

  2. 77 FR 12071 - Agency Information Collection Activities: Form I-601, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-601... Department of Homeland Security, U.S. Citizenship and Immigration Services will be submitting the following... sponsoring the collection: Form I-601. U.S. Citizenship and Immigration Services. (4) Affected public...

  3. 76 FR 58029 - Agency Information Collection Activities: Form N-600K, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-19

    ... collection was previously published in the Federal Register on June 29, 2011, at 76 FR 38197, allowing for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-600K... Information Collection Under Review: Form N- 600K; Application for Citizenship and Issuance of...

  4. 75 FR 51096 - Agency Information Collection Activities: Form N-470; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-470.... 1615-0056. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will...: Form N-470; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  5. 76 FR 17144 - Agency Information Collection Activities: Form N-300; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-28

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-300.... The Department Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting... collection: Form N-300; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will...

  6. 77 FR 27473 - Agency Information Collection Activities: Form I-924; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-924... Department of Homeland Security (DHS), U.S. Citizenship and Immigration Services (USCIS) will be submitted... sponsoring the collection: Form I-924; U.S. Citizenship and Immigration Services. (4) Affected public...

  7. 77 FR 34398 - Agency Information Collection Activities: Form N-565, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ..., 2012, at 77 FR 18255, allowing for a 60-day public comment period. USCIS did not receive any comments... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-565... Information Collection Under Review: Form N- 565, Application for Replacement...

  8. 77 FR 12072 - Agency Information Collection Activities: Form I-824; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-824... Homeland Security, U.S. Citizenship and Immigration Services (USCIS) has submitted the following... collection: Form I-824; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will...

  9. 75 FR 6212 - Agency Information Collection Activities: Form I-129, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-129... Department of Homeland Security, U.S. Citizenship and Immigration Services has submitted the following... Security sponsoring the collection: Form I-129. U.S. Citizenship and Immigration Services. (4)...

  10. 78 FR 5477 - Agency Information Collection Activities: InfoPass System, No Form Number; Extension, Without...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-25

    ... information collection notice was previously published in the Federal Register on October 31, 2012, at 77 FR... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: InfoPass... Collection. (2) Title of the Form/Collection: InfoPass System. (3) Agency form number, if any, and...

  11. Tunable growth of nanodendritic silver by galvanic-cell mechanism on formed activated carbon.

    PubMed

    Wang, Fei; Lai, Yijian; Zhao, Binyuan; Hu, Xiaobin; Zhang, Di; Hu, Keao

    2010-06-01

    Well-defined silver dendritic nanostructures have been prepared in large quantities in an ambient environment using formed activated carbon (FAC) only. A reasonable mechanism (step 1: reduction by surface reductive groups; step 2: growing in the form of a galvanic cell) is suggested.

  12. 77 FR 21104 - Agency Information Collection Activities: Form I-694, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-09

    ... a brief abstract: Primary: Individuals and households. USCIS uses the information provided on Form I... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-694, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice...

  13. 75 FR 29780 - Agency Information Collection Activities: Form I-612, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-27

    ... the Federal Register on March 12, 2010, at 74 FR 11898, allowing for a 60-day public comment period... abstract: Primary: Individuals or Households. Form I- 612 is used by USCIS to determine eligibility for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form...

  14. 76 FR 11808 - Agency Information Collection Activities: Form I-590, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... abstract: Primary: Individuals or Households. Form I- 590 provides a uniform method for applicants to apply... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-590, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  15. 76 FR 20361 - Agency Information Collection Activities: Form I-694, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-694, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 694, Notice of Appeal of Decision Under Section 210 or 245A;...

  16. 77 FR 9259 - Agency Information Collection Activities: Form I-361, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-16

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-361, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 361, Affidavit of Financial Support and Intent To Petition...

  17. 75 FR 70016 - Agency Information Collection Activities: Form I-566, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-566, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form I- 566, Interagency Record of Individual Requesting...

  18. 76 FR 66944 - Agency Information Collection Activities: Form I-129F; Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-28

    ... SECURITY Citizenship and Immigration Services Agency Information Collection Activities: Form I-129F; Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 129F, Petition for Alien Fiance(e). OMB Control No....

  19. 75 FR 29779 - Agency Information Collection Activities: Form I-918, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-27

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-918, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form I- 918, Petition for U Nonimmigrant Status; and Supplement A and...

  20. 75 FR 76745 - Agency Information Collection Activities: Form I-601, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-09

    ... 75 FR 74071, mistakenly announcing the revision of the Form I-601. The 60-day notice should have... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-601, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice...

  1. 76 FR 16800 - Agency Information Collection Activities: Form I-601, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-601, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form I- 601, Application for Waiver of Grounds of Inadmissibility;...

  2. 76 FR 71056 - Agency Information Collection Activities: Form I-566, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ... Information Collection Under Review: Form I- 566, Interagency Record of Request, A, G or NATO Dependent... Federal Register on August 18, 2011, at 76 FR 51382, allowing for a 60-day public comment period. USCIS... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form...

  3. 75 FR 41215 - Agency Information Collection Activities: Form I-821, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... required to respond, as well as a brief abstract: Primary: Individuals or Households. Form I- 821 is... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-821, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  4. 75 FR 37821 - Agency Information Collection Activities: Form I-751, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-30

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-751, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 751, Petition to Remove Conditions on Residence; OMB Control...

  5. 76 FR 45845 - Agency Information Collection Activities: Form I-777, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... published in the Federal Register on May 19, 2011, at 76 FR 28800, allowing for a 60-day public comment...: Primary: Individuals or Households. Form I- 777 is used by applicants applying for a Northern Marina... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form...

  6. 76 FR 20361 - Agency Information Collection Activities: Form I-907, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-907, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-day notice of information collection under review: Form I- 907, Request for Premium Processing Service; OMB Control No....

  7. 75 FR 11898 - Agency Information Collection Activities: Form I-612, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-612, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 612, Application for Waiver of the Foreign Residence...

  8. 75 FR 74071 - Agency Information Collection Activities: Form I-601, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-30

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-601, Revision of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 601, Application for Waiver of Grounds of Inadmissibility;...

  9. 76 FR 66946 - Agency Information Collection Activities: Form I-539, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-28

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-539, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 539, Application to Extend/Change Nonimmigrant Status....

  10. 75 FR 57049 - Agency Information Collection Activities: Form I-777, Application for Replacement of Northern...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-17

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-777, Application for Replacement of Northern Mariana Card ACTION: Correction to 30-day notice of Information Collection Under Review: Form I-777, Application for Replacement of Northern Mariana Card; OMB Control...

  11. 76 FR 52961 - Agency Information Collection Activities: Form N-300; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-24

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-300.... The Department Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting... collection: Form N-300; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will...

  12. 76 FR 70747 - Agency Information Collection Activities: Form I-90; Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-90... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the...: Form I-90; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  13. 76 FR 9805 - Agency Information Collection Activities: Form G-845 and Supplement; Revision of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-22

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-845 and.... Citizenship and Immigration Services (USCIS) will be submitting the following information collection request... collection: Form G-845 and Supplement. U.S. Citizenship and Immigration Services. (4) Affected public...

  14. 76 FR 48874 - Agency Information Collection Activities: Form G-884, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... the Federal Register on March 17, 2011, at 76 FR 28444, allowing for a 60- day public comment period... SECURITY Citizenship and Immigration Services Agency Information Collection Activities: Form G-884... Collection Under Review: Form G- 884, Request for the Return of Original Document(s). The Department...

  15. 76 FR 39415 - Agency Information Collection Activities: Form N-644, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... was previously published in the Federal Register on April 19, 2011, at 76 FR 21913, allowing for a 60... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-644... Information Collection Under Review: Form N- 644, Application for Posthumous Citizenship; OMB Control No....

  16. 75 FR 70278 - Agency Information Collection Activities: Form N-600, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... collection was previously published in the Federal Register on August 18, 2010, at 75 FR 51094, allowing for... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-600... Information Collection Under Review: Form N- 600, Application for Certificate of Citizenship; OMB Control...

  17. 76 FR 45844 - Agency Information Collection Activities: Form N-426, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... published in the Federal Register on May 10, 2011, at 76 FR 27078, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-426... Information Collection Under Review: Form N- 426, Request for Certification of Military or Naval Service....

  18. 77 FR 128 - Agency Information Collection Activities: Form N-600, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... published in the Federal Register on September 27, 2011, at 76 FR 59710, allowing for a 60-day public... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-600... Information Collection Under Review: Form N- 600, Application for Certificate of Citizenship. The...

  19. 75 FR 41216 - Agency Information Collection Activities: Form N-644, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... was previously published in the Federal Register on April 22, 2010, at 75 FR 21013, allowing for a 60... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-644... Information Collection Under Review: Form N- 644, Application for Posthumous Citizenship; OMB Control No....

  20. 75 FR 43535 - Agency Information Collection Activities: Form N-644, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-26

    ... notice in the Federal Register at 75 FR 41216 extending the use of Form N-644. However, USCIS should have... was previously published in the Federal Register on April 22, 2010, at 75 FR 21013, allowing for a 60... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form...

  1. 78 FR 73875 - Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-09

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68) AGENCY: U.S. Customs and Border Protection (CBP), Department of Homeland... requirement concerning the Canadian Border Boat Landing Permit (Form I-68). This request for comment is...

  2. 75 FR 70016 - Agency Information Collection Activities: Form I-290B, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-290B, Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection under Review: Form I- 290B,...

  3. 77 FR 17085 - Agency Information Collection Activities: Form I-612; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-612; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form I-...

  4. 76 FR 43336 - Agency Information Collection Activities: Form AR-11, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... published in the Federal Register on May 10, 2011, at 76 FR 27077 allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form AR-11... Collection under Review: Form AR- 11, Alien's Change of Address Card; OMB Control No. 1615-0007....

  5. Effect of chemical form of selenium on tissue glutathione peroxidase activity in developing rats

    NASA Technical Reports Server (NTRS)

    Lane, Helen W.; Strength, Ralph; Johnson, Janet; White, Marguerite T.

    1991-01-01

    The hypothesis that the stage of development of rats may affect the availability of various forms of selenium for the activity of glutathione peroxidase (GSHPx) in the rat was experimentally investigated. One experiment evaluated the availability of selenium as selenite or selenomethionine for GSPHx activity during three developmental states in rats: fetus and 7-day old and 14-day old nursing pups. In all tissues studied, GSHPx activity was highest in the 14-day-old pups whose dams were in the selenomethionine group. Rat pups given intraperitoneal selenite had higher liver and kidney GSHPx activity than pups given the same amount of selenium as intraperitoneal selenomethionine. In a second experiment, all dams were fed the same basal diet and pups were weaned to diets containing one of two levels of selenium and one of three forms of selenium (selenite, selenomethionine, or selenocystine). The results also supported the hypothesis these dietary forms of selenium are differentially available for GSHPx activity.

  6. Cellulase occurs in multiple active forms in ripe avocado fruit mesocarp.

    PubMed

    Kanellis, A K; Kalaitzis, P

    1992-02-01

    The existence of multiple forms of avocado (Persea americana Mill. cv Hass) cellulase in crude protein extracts of ripe avocado fruit is reported. Cellulase was separated into at least 11 multiple forms by native isoelectric focusing in the pH range between 4 and 7 and visualized by both activity staining using Congo red and immunostaining. The enzyme components were acidic proteins with isoelectric points in the range of pH 5.10 to 6.80, the predominant forms having isoelectric points of 5.60, 5.80, 5.95, and 6.20. All 11 forms were immunologically related with molecular masses of 54 kilodaltons.

  7. Education Technologies in Addressing the Problem of Forming the Socially Active Individual

    ERIC Educational Resources Information Center

    Popova, Irina N.

    2016-01-01

    The article is devoted to the analysis of technological support of the educational process in solving the problem of forming the socially active individual. The authors studied the value of the category "social activity" and analyzed educational technologies that have an impact on its formation. The obtained results gave the possibility…

  8. Morphology and optical properties of aluminum oxide formed into oxalic electrolyte with addition surface active agents

    NASA Astrophysics Data System (ADS)

    Kazarkin, B.; Stsiapanau, A.; Zhilinski, V.; Chernik, A.; Bezborodov, V.; Kozak, G.; Danilovich, S.; Smirnov, A.

    2016-08-01

    The article discusses the results of investigations of porous films of alumina, formed into oxalic electrolyte with addition surface active agents, in particular, ordering structure, roughness of a surface, the optical transparency of the electrolyte concentration and surface active agents. Also discusses the features of the formation of porous films of temperature and IR radiation.

  9. Comparing Two Forms of Concept Map Critique Activities to Facilitate Knowledge Integration Processes in Evolution Education

    ERIC Educational Resources Information Center

    Schwendimann, Beat A.; Linn, Marcia C.

    2016-01-01

    Concept map activities often lack a subsequent revision step that facilitates knowledge integration. This study compares two collaborative critique activities using a Knowledge Integration Map (KIM), a form of concept map. Four classes of high school biology students (n?=?81) using an online inquiry-based learning unit on evolution were assigned…

  10. Differential cellulolytic activity of native-form and C-terminal tagged-form cellulase derived from coptotermes formosanus and expressed in E. coli

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The endogenous cellulase gene (CfEG3a) of Coptotermes formosanus, an economically important pest termite, was cloned and overexpressed in both native form (nCfEG) and C-terminal His-tagged form (tCfEG) in E.coli. Both forms of recombinant cellulases showed hydrolytic activity on cellulosic substrate...

  11. 75 FR 42747 - Smart Grid Update; Notice of Commissioner and Staff Attendance at FERC/NARUC Collaborative on...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Smart Grid Update; Notice of Commissioner and Staff Attendance at FERC/NARUC Collaborative on Smart Response Meeting July 15, 2010. The Federal Energy Regulatory Commission hereby...

  12. 75 FR 15707 - Notice of FERC Staff Attendance at the Southwest Power Pool Board of Directors/Members Committee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-30

    ... Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool Board of... notice that members of its staff may attend the meeting of the Southwest Power Pool (SPP) Board of... address matters at issue in the following proceedings: Docket No. EL09-40, Southwest Power Pool,...

  13. 76 FR 49764 - Notice of FERC Staff Attendance at the Entergy ICT Transmission Planning Summit and Entegry...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy ICT Transmission Planning... Commission's ongoing outreach efforts. Entergy ICT Transmission Planning Summit August 23, 2011 (8 a.m.-5...

  14. 75 FR 23262 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting and the Entergy Regional State Committee Meeting April 26, 2010. The Federal Energy Regulatory Commission hereby...

  15. 75 FR 63168 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting and the Entergy Regional State Committee Meeting October 6, 2010. The Federal Energy Regulatory Commission...

  16. 76 FR 13613 - Notice of FERC Staff Attendance at the ICT Stakeholders Policy Committee and Entergy Regional...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the ICT Stakeholders Policy Committee and Entergy Regional State Committee Meetings The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its...

  17. 75 FR 41858 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting July 12, 2010. The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend...

  18. 77 FR 3765 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ... Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and Stakeholder Meeting The Federal Energy Regulatory Commission hereby gives notice that members... outreach efforts. Entergy Regional State Committee Working Group and Stakeholders Meeting January 26,...

  19. 76 FR 67727 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and Stakeholder Meeting The Federal Energy Regulatory Commission hereby gives notice that...

  20. 76 FR 62804 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work... outreach efforts. Entergy Regional State Committee Work Group and Stakeholder Meeting October 19, 2011 (9...

  1. Functional Specificity of the Visual Word Form Area: General Activation for Words and Symbols but Specific Network Activation for Words

    ERIC Educational Resources Information Center

    Reinke, Karen; Fernandes, Myra; Schwindt, Graeme; O'Craven, Kathleen; Grady, Cheryl L.

    2008-01-01

    The functional specificity of the brain region known as the Visual Word Form Area (VWFA) was examined using fMRI. We explored whether this area serves a general role in processing symbolic stimuli, rather than being selective for the processing of words. Brain activity was measured during a visual 1-back task to English words, meaningful symbols…

  2. Process for forming a homogeneous oxide solid phase of catalytically active material

    DOEpatents

    Perry, Dale L.; Russo, Richard E.; Mao, Xianglei

    1995-01-01

    A process is disclosed for forming a homogeneous oxide solid phase reaction product of catalytically active material comprising one or more alkali metals, one or more alkaline earth metals, and one or more Group VIII transition metals. The process comprises reacting together one or more alkali metal oxides and/or salts, one or more alkaline earth metal oxides and/or salts, one or more Group VIII transition metal oxides and/or salts, capable of forming a catalytically active reaction product, in the optional presence of an additional source of oxygen, using a laser beam to ablate from a target such metal compound reactants in the form of a vapor in a deposition chamber, resulting in the deposition, on a heated substrate in the chamber, of the desired oxide phase reaction product. The resulting product may be formed in variable, but reproducible, stoichiometric ratios. The homogeneous oxide solid phase product is useful as a catalyst, and can be produced in many physical forms, including thin films, particulate forms, coatings on catalyst support structures, and coatings on structures used in reaction apparatus in which the reaction product of the invention will serve as a catalyst.

  3. 75 FR 5099 - Agency Information Collection Activities: Visa Waiver Program Carrier Agreement (Form I-775)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... previously published in the Federal Register (74 FR 60281) on November 20, 2009, allowing for a 60-day... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Visa Waiver Program... approval in accordance with the Paperwork Reduction Act: Visa Waiver Program Carrier Agreement (Form...

  4. 77 FR 49453 - Agency Information Collection Activities: Application for Employment Authorization, Form I-765...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-16

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Application... Security (DHS), U.S. Citizenship and Immigration Services (USCIS), submitted the following emergency... applicable component of the Department of Homeland Security sponsoring the collection: Form I-765, USCIS....

  5. 75 FR 57480 - Agency Information Collection Activities: Customs Declaration (Form 6059B)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-21

    ... SECURITY U.S. Customs And Border Protection Agency Information Collection Activities: Customs Declaration (Form 6059B) AGENCY: U.S. Customs and Border Protection (CBP), Department of Homeland Security. ACTION... the Customs Declaration. This request for comment is being made pursuant to the Paperwork...

  6. 75 FR 71452 - Agency Information Collection Activities: Customs Declaration (Form 6059B)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-23

    ... published in the Federal Register (75 FR 57480) on September 21, 2010, allowing for a 60-day comment period... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Customs Declaration (Form 6059B) AGENCY: U.S. Customs and Border Protection, Department of Homeland Security. ACTION:...

  7. 75 FR 3245 - Agency Information Collection Activities: Aircraft/Vessel Report (Form I-92)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-20

    ... published in the Federal Register (74 FR 54839) on October 23, 2009, allowing for a 60-day comment period... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Aircraft/Vessel... approval in accordance with the Paperwork Reduction Act: Aircraft/ Vessel Report (Form I-92). This is...

  8. 77 FR 2561 - Agency Information Collection Activities: Passenger List/Crew List (CBP Form I-418)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-18

    ... of automated collection techniques or the use of other forms of information technology; and (e... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Passenger List/Crew.... ACTION: 60-Day notice and request for comments; Extension of an existing information collection:...

  9. 75 FR 30050 - Agency Information Collection Activities: Form N-648, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-28

    ... collection was previously published in the Federal Register on February 1, 2010, at 75 FR 5099, allowing for... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-648... No. 1615-0060. The Department of Homeland Security, U.S. Citizenship and Immigration Services...

  10. 77 FR 3484 - Agency Information Collection Activities: Form I-914, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-24

    ... Federal Register on October 28, 2011, at 76 FR 66944, allowing for a 60-day public comment period. USCIS... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-914... Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the following...

  11. 77 FR 34053 - Agency Information Collection Activities: Form I-590, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-08

    ... published in the Federal Register on March 12, 2012, at 77 FR 14535, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-590... of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  12. 75 FR 47822 - Agency Information Collection Activities: Form G-639, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... collection was previously published in the Federal Register on May 4, 2010, at 75 FR 23785, allowing for a 60... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-639.... 1615- 0102. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS)...

  13. 76 FR 53929 - Agency Information Collection Activities: Form G-639, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ... published in the Federal Register on May 3, 2011, at 76 FR 24908, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-639... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  14. 76 FR 12750 - Agency Information Collection Activities: Form I-829, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ... collection was previously published in the Federal Register on November 30, 2010, at 75 FR 74070, allowing... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-829... No. 1615-0045. The Department of Homeland Security, U.S. Citizenship and Immigration Services...

  15. 75 FR 47824 - Agency Information Collection Activities: Form I-643, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ..., 2010, at 75 FR 23784, allowing for a 60-day public comment period. USCIS did not receive any comments... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-643.../Asylee Adjusting Status; OMB Control No. 1615-0070. The Department of Homeland Security, U.S....

  16. 77 FR 12071 - Agency Information Collection Activities: Form G-28, Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... notice was previously published in the Federal Register on October 12, 2011, at 76 FR 63322, allowing for... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-28... Representative. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will...

  17. 77 FR 27474 - Agency Information Collection Activities: Form I-687; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-687...-687. The Department Homeland Security (DHS), U.S. Citizenship and Immigration Services (USCIS) has...-687; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  18. 77 FR 59205 - Agency Information Collection Activities: Application for Posthumous Citizenship, Form Number N...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    ..., at 77 FR 43346, allowing for a 60-day public comment period. USCIS did not receive any comment in... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Application for Posthumous Citizenship, Form Number N-644; Extension, Without Change, of a Currently...

  19. 77 FR 12070 - Agency Information Collection Activities: Form I-102; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-102... Document. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be... directed to the Department of Homeland Security (DHS), U.S. Citizenship and Immigration Services...

  20. 77 FR 50710 - Agency Information Collection Activities: Employment Eligibility Verification, Form I-9, OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ... information collection was previously published in the Federal Register on March 27, 2012, at 77 FR 18256... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Employment... Verification, Form I-9. The Department of Homeland Security (DHS), U.S. Citizenship and Immigration...

  1. 75 FR 51094 - Agency Information Collection Activities: Form I-363, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    .... The information collection was previously published in the Federal Register on June 23, 2010, at 75 FR... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-363... Amerasian; OMB Control No. 1615-0022. The Department of Homeland Security, U.S. Citizenship and...

  2. 77 FR 27474 - Agency Information Collection Activities: Form N-648, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-648... Department of Homeland Security (DHS), U.S. Citizenship and Immigration Services (USCIS) will be submitting.... Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked or required to respond,...

  3. 76 FR 12751 - Agency Information Collection Activities: Form I-589, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ... information collection was previously published in the Federal Register on November 30, 2010, at 75 FR 74069... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-589... Control No. 1615-0067. The Department of Homeland Security, U.S. Citizenship and Immigration...

  4. 76 FR 27078 - Agency Information Collection Activities: Form N-426, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-10

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-426... Control No. 1615-0053. The Department of Homeland Security, U.S. Citizenship and Immigration Services.... Citizenship and Immigration Services. (4) Affected public who will be asked or required to respond, as well...

  5. 76 FR 63320 - Agency Information Collection Activities: Form N-470, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... Federal Register on August 12, 2011, at 76 FR 50237, for a 60-day public comment period. Subsequently... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-470... Department of Homeland Security, U.S. Citizenship and Immigration Services will be submitting the...

  6. 76 FR 78674 - Agency Information Collection Activities: Form N-470, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    ... information collection notice was previously published in the Federal Register on October 12, 2011, at 76 FR... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-470... Control No. 1615-0056. The Department of Homeland Security, U.S. Citizenship and Immigration...

  7. 77 FR 34052 - Agency Information Collection Activities: Form I-102; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-08

    ... was previously published in the Federal Register on February 28, 2012 at 77 FR 12070, allowing for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-102... Document. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will...

  8. 75 FR 10298 - Agency Information Collection Activities: Form I-212; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-05

    ... 24, 2009, at 74 FR 61358, allowing for a 60-day public comment period. USCIS received one comment for... SECURITY Citizenship and Immigration Services Agency Information Collection Activities: Form I-212.... Citizenship and Immigration Services (USCIS) has submitted the following information collection request to...

  9. 76 FR 36560 - Agency Information Collection Activities; Form N-300; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-22

    ... was previously published in the Federal Register on March 28, 2011 at 76 FR 17144, allowing for a 60... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities; Form N-300.... The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will...

  10. 77 FR 31033 - Agency Information Collection Activities: Form I-589, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-24

    ... published in the Federal Register on March 19, 2012, at 77 FR 16047, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-589... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  11. 77 FR 23270 - Agency Information Collection Activities: Form I-290B, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ... Register on February 7, 2012, at 77 FR 6134, allowing for a 60-day public comment period. USCIS received... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-290B.... Citizenship and Immigration Services (USCIS) will be ] submitting the following information collection...

  12. 78 FR 26647 - Agency Information Collection Activities: Application To Replace Permanent Resident Card, Form I...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-07

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Application To... Notice. SUMMARY: The Department of Homeland Security (DHS), U.S. Citizenship and Immigration Services... Security sponsoring the collection: Form I-90, U.S. Citizenship and Immigration Services. (4)...

  13. 75 FR 10298 - Agency Information Collection Activities: Form I-929; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-05

    ... collection was previously published in the Federal Register on November 24, 2009, at 74 FR 61360, allowing... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-929... No. 1615-0106. The Department of Homeland Security, U.S. Citizenship and Immigration Services...

  14. 75 FR 39271 - Agency Information Collection Activities: Form I-694, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-08

    ... Federal Register on April 22, 2010, at 75 FR 21014, allowing for a 60-day public comment period. USCIS did... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-694....S. Citizenship and Immigration Services (USCIS) will be submitting the following...

  15. 76 FR 46895 - Agency Information Collection Activity Under OMB Review; Reports, Forms and Record Keeping...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... National Highway Traffic Safety Administration Agency Information Collection Activity Under OMB Review; Reports, Forms and Record Keeping Requirements AGENCY: National Highway Traffic Safety Administration, U.S... Highway Traffic Safety Administration Title: Side Impact Phase in Reporting Requirements--Part 597...

  16. 77 FR 33759 - Agency Information Collection Activities: Form I-601, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... previously published in the Federal Register on February 28, 2012, at 77 FR 12071, allowing for a 60-day... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-601, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  17. 78 FR 40490 - Agency Information Collection Activities: Petition for a Nonimmigrant Worker, Form I-129...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-05

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Petition for a Nonimmigrant Worker, Form I-129; Revision of a Currently Approved Collection ACTION: 60-Day Notice...: I-129; USCIS. (4) Affected public who will be asked or required to respond, as well as a...

  18. 75 FR 12250 - Agency Information Collection Activities: Form I-191, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... 74 FR 61359 allowing for a 60-day public comment period. USCIS did not receive any comments for this... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-191, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  19. 76 FR 40385 - Agency Information Collection Activities: Form I-907, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-08

    ... the Federal Register on April 12, 2011, at 76 FR 20361, allowing for a 60-day public comment period... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-907, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day notice...

  20. 77 FR 23734 - Agency Information Collection Activities: Form I-361, Extension of a Currently Approved...

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    2012-04-20

    ..., 2012, at 77 FR 9259, allowing for a 60-day public comment period. USCIS did not receive any comments... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-361, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  1. 75 FR 52541 - Agency Information Collection Activities: Form I-865, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-26

    ... was previously published in the Federal Register on June 9, 2010, at 75 FR 32801, allowing for a 60... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-865, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  2. 75 FR 52541 - Agency Information Collection Activities: Form I-243, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-26

    ... published in the Federal Register on June 9, 2010, at 75 FR 32799, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-243, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  3. 76 FR 43335 - Agency Information Collection Activities: Form I-765, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... collection was previously published in the Federal Register on April 19, 2011, at 76 FR 21912 allowing for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-765, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  4. 77 FR 35419 - Agency Information Collection Activities: Form I-601, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-13

    ...) published a 30-day information collection notice in the Federal Register at 77 FR 33759, allowing for an... previously published in the Federal Register on February 28, 2012, at 77 FR 12071, allowing for a 60-day... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form...

  5. 76 FR 69275 - Agency Information Collection Activities: Form I-192, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-08

    ... the Federal Register on August 12, 2011, at 76 FR 50239, allowing for a 60- day public comment period... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-192, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  6. 75 FR 41215 - Agency Information Collection Activities: Form I-687, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... Federal Register on April 23, 2010, at 75 FR 21340, allowing for a 60-day public comment period. USCIS did... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-687, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  7. 75 FR 65022 - Agency Information Collection Activities: Form I-751, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-21

    ... collection was previously published in the Federal Register on June 30, 2010, at 75 FR 37821, allowing for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-751.... 1615- 0038. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS)...

  8. 76 FR 60852 - Agency Information Collection Activities; Form I-130; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-30

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities; Form I-130... of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the... Department of Homeland Security (DHS), U.S. Citizenship and Immigration Services (USCIS), Chief,...

  9. 76 FR 31971 - Agency Information Collection Activities: Form I-212; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    ... on March 16, 2011, at 76 FR 14419, allowing for a 60-day public comment period. USCIS received no... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-212.... Citizenship and Immigration Services (USCIS) will be submitting the following information collection...

  10. 76 FR 72209 - Agency Information Collection Activities: Form N-300; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-22

    ... the Federal Register on August 24, 2011 at 76 FR 52961, allowing for a 60-day public comment period... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-300... Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the...

  11. 77 FR 2560 - Agency Information Collection Activities: Form I-90, Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-18

    ... previously published in the Federal Register on November 15, 2011, at 76 FR 70747, allowing for a 60-day... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-90... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  12. 77 FR 3486 - Agency Information Collection Activities: Form I-539, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-24

    ... previously published in the Federal Register on October 28, 2011, at 76 FR 66946, allowing for a 60-day... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-539... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  13. 77 FR 3485 - Agency Information Collection Activities: Form I-129F, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-24

    ... Federal Register on October 28, 2011, at 76 FR 66944, allowing for a 60-day public comment period. USCIS... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-129F... Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the following...

  14. 76 FR 70747 - Agency Information Collection Activities: Form I-526, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... published in the Federal Register on August 12, 2011, at 76 FR 50238, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-526... Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the...

  15. 75 FR 65022 - Agency Information Collection Activities: Form I-698, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-21

    .... The information collection was previously published in the Federal Register on June 23, 2010, at 75 FR... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-698... Resident; OMB Control No. 1615-0035. The Department of Homeland Security, U.S. Citizenship and...

  16. Comparing the Long and Short Forms of the Student Version of the Jenkins Activity Survey.

    ERIC Educational Resources Information Center

    Yarnold, Paul R.; And Others

    This paper reports on a short version of the Student Jenkins Activity Survey (JAS), a multiple choice questionnaire that measures Type A "coronary-prone" behavior in assessing subjects' A/B types. The primary objective was to determine if the short and long forms of the student JAS represent similar measurement instruments. A secondary objective…

  17. 77 FR 65708 - Agency Information Collection Activities: Petition To Remove the Conditions on Residence, Form...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Petition To...: 60-Day Notice. SUMMARY: The Department of Homeland Security (DHS), U.S. Citizenship and Immigration... by persons who assist the respondent with the preparation of the form or the cost to the...

  18. 76 FR 37059 - Agency Information Collection Activities: Proposed Collection; Comment Request-Form FNS-339...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    ..., Infants and Children (WIC); the WIC Farmers' Market Nutrition Program (FMNP); and/or the Senior Farmers... Food and Nutrition Service Agency Information Collection Activities: Proposed Collection; Comment Request--Form FNS-339, Federal-State Supplemental Nutrition Program(s) Agreement AGENCY: Food...

  19. 76 FR 2917 - Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-18

    ... proposed information collection was previously published in the Federal Register (75 FR 61508) on October 5... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68) AGENCY: U.S. Customs and Border Protection, Department of Homeland...

  20. Project Activities as a Form of English Language Teaching Based on the Interdisciplinary Approach to Form Intercultural Communicative Competence

    ERIC Educational Resources Information Center

    Redchenko, Nadezhda N.

    2016-01-01

    The authors of this article suggest a thesis about the purpose of teaching a foreign language--it is student's communicative activities, i.e. learning a foreign language in practice. The teacher's task is to encourage activities of every student and to create situations to develop their creative activities in a learning process. New information…

  1. PROCESS FOR THE PRODUCTION OF AN ACTIVATED FORM OF UO$sub 2$

    DOEpatents

    Polissar, M.J.

    1957-09-24

    A process for producing a highly active form of UO/sub 2/ characterized both by rapid oxidation in air and by rapid chlorination with CCl/sub 4/ vapor at an elevated temperature is reported. In accordance with the process, commercial UO/sub 2/, is subjected to a series of oxidation-reduction operations to produce a form of UC/sub 2/ of enhanced reactivity. By treatimg commercial UO/sub 2/ at a temperature between 335 and 485 deg C with methane, then briefly with an oxygen containing gas and followimg this by a second treatment with a methane containing gas, the original relatively stable charge of UO/sub 2/ will be transformed into an active form of UO/sub 2/.

  2. Binary Toxin Subunits of Lysinibacillus sphaericus Are Monomeric and Form Heterodimers after In Vitro Activation.

    PubMed

    Surya, Wahyu; Chooduang, Sivadatch; Choong, Yeu Khai; Torres, Jaume; Boonserm, Panadda

    2016-01-01

    The binary toxin from Lysinibacillus sphaericus has been successfully used for controlling mosquito-transmitted diseases. An activation step shortens both subunits BinA and BinB before their interaction with membranes and internalization in midgut cells, but the precise role of this activation step is unknown. Herein, we show conclusively using three orthogonal biophysical techniques that protoxin subunits form only monomers in aqueous solution. However, in vitro activated toxins readily form heterodimers. This oligomeric state did not change after incubation of these heterodimers with detergent. These results are consistent with the evidence that maximal toxicity in mosquito larvae is achieved when the two subunits, BinA and BinB, are in a 1:1 molar ratio, and directly link proteolytic activation to heterodimerization. Formation of a heterodimer must thus be necessary for subsequent steps, e.g., interaction with membranes, or with a suitable receptor in susceptible mosquito species. Lastly, despite existing similarities between BinB C-terminal domain with domains 3 and 4 of pore-forming aerolysin, no aerolysin-like SDS-resistant heptameric oligomers were observed when the activated Bin subunits were incubated in the presence of detergents or lipidic membranes.

  3. Binary Toxin Subunits of Lysinibacillus sphaericus Are Monomeric and Form Heterodimers after In Vitro Activation

    PubMed Central

    Choong, Yeu Khai; Torres, Jaume; Boonserm, Panadda

    2016-01-01

    The binary toxin from Lysinibacillus sphaericus has been successfully used for controlling mosquito-transmitted diseases. An activation step shortens both subunits BinA and BinB before their interaction with membranes and internalization in midgut cells, but the precise role of this activation step is unknown. Herein, we show conclusively using three orthogonal biophysical techniques that protoxin subunits form only monomers in aqueous solution. However, in vitro activated toxins readily form heterodimers. This oligomeric state did not change after incubation of these heterodimers with detergent. These results are consistent with the evidence that maximal toxicity in mosquito larvae is achieved when the two subunits, BinA and BinB, are in a 1:1 molar ratio, and directly link proteolytic activation to heterodimerization. Formation of a heterodimer must thus be necessary for subsequent steps, e.g., interaction with membranes, or with a suitable receptor in susceptible mosquito species. Lastly, despite existing similarities between BinB C-terminal domain with domains 3 and 4 of pore-forming aerolysin, no aerolysin-like SDS-resistant heptameric oligomers were observed when the activated Bin subunits were incubated in the presence of detergents or lipidic membranes. PMID:27341696

  4. Cytochrome P450-mediated activation of the fragrance compound geraniol forms potent contact allergens

    SciTech Connect

    Hagvall, Lina; Baron, Jens Malte; Boerje, Anna; Weidolf, Lars; Merk, Hans; Karlberg, Ann-Therese

    2008-12-01

    Contact sensitization is caused by low molecular weight compounds which penetrate the skin and bind to protein. In many cases, these compounds are activated to reactive species, either by autoxidation on exposure to air or by metabolic activation in the skin. Geraniol, a widely used fragrance chemical, is considered to be a weak allergen, although its chemical structure does not indicate it to be a contact sensitizer. We have shown that geraniol autoxidizes and forms allergenic oxidation products. In the literature, it is suggested but not shown that geraniol could be metabolically activated to geranial. Previously, a skin-like CYP cocktail consisting of cutaneous CYP isoenzymes, was developed as a model system to study cutaneous metabolism. In the present study, we used this system to investigate CYP-mediated activation of geraniol. In incubations with the skin-like CYP cocktail, geranial, neral, 2,3-epoxygeraniol, 6,7-epoxygeraniol and 6,7-epoxygeranial were identified. Geranial was the main metabolite formed followed by 6,7-epoxygeraniol. The allergenic activities of the identified metabolites were determined in the murine local lymph node assay (LLNA). Geranial, neral and 6,7-epoxygeraniol were shown to be moderate sensitizers, and 6,7-epoxygeranial a strong sensitizer. Of the isoenzymes studied, CYP2B6, CYP1A1 and CYP3A5 showed high activities. It is likely that CYP1A1 and CYP3A5 are mainly responsible for the metabolic activation of geraniol in the skin, as they are expressed constitutively at significantly higher levels than CYP2B6. Thus, geraniol is activated through both autoxidation and metabolism. The allergens geranial and neral are formed via both oxidation mechanisms, thereby playing a large role in the sensitization to geraniol.

  5. Cytochrome P450-mediated activation of the fragrance compound geraniol forms potent contact allergens.

    PubMed

    Hagvall, Lina; Baron, Jens Malte; Börje, Anna; Weidolf, Lars; Merk, Hans; Karlberg, Ann-Therese

    2008-12-01

    Contact sensitization is caused by low molecular weight compounds which penetrate the skin and bind to protein. In many cases, these compounds are activated to reactive species, either by autoxidation on exposure to air or by metabolic activation in the skin. Geraniol, a widely used fragrance chemical, is considered to be a weak allergen, although its chemical structure does not indicate it to be a contact sensitizer. We have shown that geraniol autoxidizes and forms allergenic oxidation products. In the literature, it is suggested but not shown that geraniol could be metabolically activated to geranial. Previously, a skin-like CYP cocktail consisting of cutaneous CYP isoenzymes, was developed as a model system to study cutaneous metabolism. In the present study, we used this system to investigate CYP-mediated activation of geraniol. In incubations with the skin-like CYP cocktail, geranial, neral, 2,3-epoxygeraniol, 6,7-epoxygeraniol and 6,7-epoxygeranial were identified. Geranial was the main metabolite formed followed by 6,7-epoxygeraniol. The allergenic activities of the identified metabolites were determined in the murine local lymph node assay (LLNA). Geranial, neral and 6,7-epoxygeraniol were shown to be moderate sensitizers, and 6,7-epoxygeranial a strong sensitizer. Of the isoenzymes studied, CYP2B6, CYP1A1 and CYP3A5 showed high activities. It is likely that CYP1A1 and CYP3A5 are mainly responsible for the metabolic activation of geraniol in the skin, as they are expressed constitutively at significantly higher levels than CYP2B6. Thus, geraniol is activated through both autoxidation and metabolism. The allergens geranial and neral are formed via both oxidation mechanisms, thereby playing a large role in the sensitization to geraniol.

  6. In vitro and in vivo Bone-Forming Activity of Saururus chinensis Extract.

    PubMed

    Moon, Seong-Hee; Choi, Sik-Won; Park, Sang-Joon; Ryu, Shi-Yong; Hwang, Kyu-Seok; Kim, Cheol-Hee; Kim, Seong Hwan

    2015-07-01

    Bone is maintained by osteoclast-mediated resorption and osteoblast-mediated formation. Recently, anti-osteoporotic activity of Saururus chinensis extract (SCE) and anti-osteoclastogenic activity of its components have been reported, but the effect of SCE on bone formation has not been studied well. Therefore, in this study, we investigated whether Saururus chinensis SCE exhibits in vitro osteogenic and in vivo bone-forming activity. extract strongly enhanced the bone morphogenetic protein (BMP)-2-stimulated induction of alkaline phosphatase, an early phase biomarker of osteoblast differentiation, in bi-potential mesenchymal progenitor C2C12 cells. In vitro osteogenic activity of SCE was accompanied by enhanced expression of BMP-2, BMP-4, BMP-7 and BMP-9 mRNA. In addition, a pharmacological inhibition study suggested the involvement of p38 activation in the osteogenic action of SCE. Moreover, the BMP dependency and the involvement of p38 activation in the osteogenic action of SCE were confirmed by the treatment of noggin, an antagonist of BMP. Saururus chinensis extract also exhibited to induce runt-related transcription factor 2 activation at the high concentration. Furthermore, the in vivo osteogenic activity of SCE was confirmed in zebrafish and mouse calvarial bone formation models, suggesting the possibility of its use for bone formation. In conclusion, we suggested that in vivo anti-osteoporotic activity of SCE could be because of its dual action in bone, anti-osteoclastogenic and anabolic activity.

  7. [Biophysical aspects of biological activity structure--strain calcium carbonat in micellar form].

    PubMed

    Ivanov, V I; Stekhin, A A; Iakovleva, G V; Savostikova, O N; Alekseeva, A V; P'ianzina, I P

    2013-01-01

    Results of the study of electrochemical and structural state of phase of associated water in the solutions of structurally stressed calcium carbonate in the micellar form are reported. On the base on the comparison of structural--physical changes of activated water with the data on the activity of bioluminiscentic "Ecolyum" microorganisms in their noncontact activation the electronic mechanism of the effect of activated water on cellular metabolism is substantiated The use of "Micellate of calcium" possessing non-contact electron-donor action on cellular structures was shown to permit to compensate the deficit of electrons and thereby to restore the activities of reductases and iron-containing peptides required for the production of regulatory ROS and alteration in redox state of the intracellular environment. PMID:24624817

  8. Integration of active pharmaceutical ingredient solid form selection and particle engineering into drug product design.

    PubMed

    Ticehurst, Martyn David; Marziano, Ivan

    2015-06-01

    This review seeks to offer a broad perspective that encompasses an understanding of the drug product attributes affected by active pharmaceutical ingredient (API) physical properties, their link to solid form selection and the role of particle engineering. While the crucial role of active pharmaceutical ingredient (API) solid form selection is universally acknowledged in the pharmaceutical industry, the value of increasing effort to understanding the link between solid form, API physical properties and drug product formulation and manufacture is now also being recognised. A truly holistic strategy for drug product development should focus on connecting solid form selection, particle engineering and formulation design to both exploit opportunities to access simpler manufacturing operations and prevent failures. Modelling and predictive tools that assist in establishing these links early in product development are discussed. In addition, the potential for differences between the ingoing API physical properties and those in the final product caused by drug product processing is considered. The focus of this review is on oral solid dosage forms and dry powder inhaler products for lung delivery.

  9. Norovirus Proteinase-Polymerase and Polymerase Are Both Active Forms of RNA-Dependent RNA Polymerase

    PubMed Central

    Belliot, Gaël; Sosnovtsev, Stanislav V.; Chang, Kyeong-Ok; Babu, Vijay; Uche, Uzo; Arnold, Jamie J.; Cameron, Craig E.; Green, Kim Y.

    2005-01-01

    In vitro mapping studies of the MD145 norovirus (Caliciviridae) ORF1 polyprotein identified two stable cleavage products containing the viral RNA-dependent RNA polymerase (RdRp) domains: ProPol (a precursor comprised of both the proteinase and polymerase) and Pol (the mature polymerase). The goal of this study was to identify the active form (or forms) of the norovirus polymerase. The recombinant ProPol (expressed as Pro−Pol with an inactivated proteinase domain to prevent autocleavage) and recombinant Pol were purified after synthesis in bacteria and shown to be active RdRp enzymes. In addition, the mutant His-E1189A-ProPol protein (with active proteinase but with the natural ProPol cleavage site blocked) was active as an RdRp, confirming that the norovirus ProPol precursor could possess two enzymatic activities simultaneously. The effects of several UTP analogs on the RdRp activity of the norovirus and feline calicivirus Pro−Pol enzymes were compared and found to be similar. Our data suggest that the norovirus ProPol is a bifunctional enzyme during virus replication. The availability of this recombinant ProPol enzyme might prove useful in the development of antiviral drugs for control of the noroviruses associated with acute gastroenteritis. PMID:15681440

  10. An ELISA method detecting the active form of suPAR.

    PubMed

    Zhou, Xiaolei; Xu, Mingming; Huang, Hailong; Mazar, Andrew; Iqbal, Zafar; Yuan, Cai; Huang, Mingdong

    2016-11-01

    Urokinase plasminogen activator receptor (uPAR) exists in a number of formats in human plasma, including soluble uPAR (suPAR) and uPAR fragments. We developed an ELISA method to detect specifically the active form suPAR, which binds to its natural ligand uPA. The intra CV and inter CV of this ELISA assay is 8.5% and 9.6% respectively, and the assay can recover 99.74% of added recombinant suPAR from 10% plasma. This assay is quite sensitive, capable of detecting down to 15pg/ml of suPAR, and can measure suPAR concentrations in the range of 0.031-8ng/ml with high linear relationship. Plasma samples from pregnant women were also measured for the active form of suPAR with this assay, giving an averaged level of 1.39ng/ml, slightly higher than the level of pooled plasma from healthy donors (0.96ng/ml). This study demonstrates the feasibility to measure the active form of suPAR, which will likely have value in clinical applications. PMID:27591605

  11. 76 FR 53144 - Agency Information Collection Activities: Form I-508 and Form I-508F, Extension of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-25

    ... information collection was previously published in the Federal Register on June 2, 2011, at 76 FR 31972..., Exemptions and Immunities. The Department of Homeland Security, U.S. Citizenship and Immigration Services..., Privileges, Exemptions and Immunities. (3) Agency form number, if any, and the applicable component of...

  12. Linear Closed-form Solution and Finite-element Analysis of an Active Tensegrity Unit

    NASA Astrophysics Data System (ADS)

    Kmeť, Stanislav; Platko, Peter

    2012-11-01

    Results of the linear closed form solution of an active or adaptive tensegrity unit, as well as its numerical analysis using finite element method are presented in the paper. The shape of the unit is an octahedral cell with a square base and it is formed by thirteen members (four bottom and four top cables, four edge struts and one central strut). The central strut is designed as an actuator that allows for an adjustment of the shape of the unit which leads to changes of tensile forces in the cables. Due to the diagonal symmetry of the 3D tensegrity unit the closed-form analysis is based on the 2D solution of the equivalent planar biconvex cable system with one central strut under a vertical point load.

  13. Cyclotron production of ``very high specific activity'' platinum radiotracers in No Carrier Added form

    NASA Astrophysics Data System (ADS)

    Birattari, C.; Bonardi, M.; Groppi, F.; Gini, L.; Gallorini, M.; Sabbioni, E.; Stroosnijder, M. F.

    2001-12-01

    At the "Radiochemistry Laboratory" of Accelerators and Applied Superconductivity Laboratory, LASA, several production and quality assurance methods for short-lived and high specific activity radionuclides, have been developed. Presently, the irradiations are carried out at the Scanditronix MC40 cyclotron (K=38; p, d, He-4 and He-3) of JRC-Ispra, Italy, of the European Community, while both chemical purity and specific activity determination are carried out at the TRIGA MARK II research reactor of University of Pavia and at LASA itself. In order to optimize the irradiation conditions for platinum radiotracer production, both thin- and thick-target excitation function of natOs(α,xn) nuclear reactions were measured. A very selective radiochemical separation to obtain Pt radiotracers in No Carrier Added form, has been developed. Both real specific activity and chemical purity of radiotracer, have been determined by neutron activation analysis and atomic absorption spectrometry. An Isotopic Dilution Factor (IDF) of the order of 50 is achieved.

  14. Role of drop distortion in enhancing the lightning activity in clouds formed over cities

    NASA Astrophysics Data System (ADS)

    Bhalwankar, Rohini; Kamra, A. K.

    2013-03-01

    Atmospheric pollutants can modify the electrification and lightning activity in thunderclouds. Laboratory simulation experiments show that distortion of water drops is more when drops are formed from water polluted with Sulfate/Nitrate salts than that from distilled water and the difference in distortions is more in a higher electric field. Further, the polluted water drops falling in a horizontal electric field can trigger a discharge on their surface and the discharge can propagate as a streamer in lower electric fields as compared to that from distilled water drops. The difference in electrical conductivities of polluted and unpolluted water drops is most likely the key factor for manifestation of these differences. It is proposed that the enhanced distortion of polluted drops coupled with the change in their characteristics to trigger and propagate a discharge in lower electric fields may significantly contribute to the enhancement of lightning activity observed in clouds formed over big cities.

  15. A CO LINE AND INFRARED CONTINUUM STUDY OF THE ACTIVE STAR-FORMING COMPLEX W51

    SciTech Connect

    Kang, Miju; Lee, Youngung; Choi, Minho; Bieging, John H.; Kulesa, Craig A.; Peters, William L.

    2010-09-15

    We present the results of an extensive observational study of the active star-forming complex W51 that was observed in the J = 2 - 1 transition of the {sup 12}CO and {sup 13}CO molecules over a 1.{sup 0}25 x 1.{sup 0}00 region with the University of Arizona Heinrich Hertz Submillimeter Telescope. We use a statistical equilibrium code to estimate physical properties of the molecular gas. We compare the molecular cloud morphology with the distribution of infrared (IR) and radio continuum sources and find associations between molecular clouds and young stellar objects (YSOs) listed in Spitzer IR catalogs. The ratios of CO lines associated with H II regions are different from the ratios outside the active star-forming regions. We present evidence of star formation triggered by the expansion of the H II regions and by cloud-cloud collisions. We estimate that about 1% of the cloud mass is currently in YSOs.

  16. A CO Line and Infrared Continuum Study of the Active Star-forming Complex W51

    NASA Astrophysics Data System (ADS)

    Kang, Miju; Bieging, John H.; Kulesa, Craig A.; Lee, Youngung; Choi, Minho; Peters, William L.

    2010-09-01

    We present the results of an extensive observational study of the active star-forming complex W51 that was observed in the J = 2 - 1 transition of the 12CO and 13CO molecules over a 1fdg25 × 1fdg00 region with the University of Arizona Heinrich Hertz Submillimeter Telescope. We use a statistical equilibrium code to estimate physical properties of the molecular gas. We compare the molecular cloud morphology with the distribution of infrared (IR) and radio continuum sources and find associations between molecular clouds and young stellar objects (YSOs) listed in Spitzer IR catalogs. The ratios of CO lines associated with H II regions are different from the ratios outside the active star-forming regions. We present evidence of star formation triggered by the expansion of the H II regions and by cloud-cloud collisions. We estimate that about 1% of the cloud mass is currently in YSOs.

  17. Forming a negative impression of another person correlates with activation in medial prefrontal cortex and amygdala.

    PubMed

    Iidaka, Tetsuya; Harada, Tokiko; Sadato, Norihiro

    2011-09-01

    Neural correlates involved in the formation of negative impression from face were investigated using event-related functional magnetic resonance imaging and a partial conditioning paradigm. Eighteen normal volunteers underwent imaging while they viewed the faces of two unfamiliar individuals: one individual's face was partially accompanied by negative emotion but the other's was not. After the volunteers learned the relationship between the faces and the emotion, they formed a more negative impression of the person's face when the emotion was presented. Subtraction analysis of the individuals' neutral faces revealed activation in the dorsal anterior cingulate cortex and superior temporal sulcus, but this activity did not correlate with the change of impression from face. On the other hand, the response in the left amygdala negatively correlated with the change of impression from face in the first run. Time modulation analysis revealed that activity in the dorsomedial prefrontal cortex associated with negative emotion was the largest in the initial part of the acquisition. These results suggest that a negative impression from face may be formed by orchestrated activity in the dorsomedial prefrontal cortex, dorsal anterior cingulate cortex and amygdala, and that the activity has a prominent role in the initial acquisition of negative emotion. PMID:20693390

  18. Forming a negative impression of another person correlates with activation in medial prefrontal cortex and amygdala.

    PubMed

    Iidaka, Tetsuya; Harada, Tokiko; Sadato, Norihiro

    2011-09-01

    Neural correlates involved in the formation of negative impression from face were investigated using event-related functional magnetic resonance imaging and a partial conditioning paradigm. Eighteen normal volunteers underwent imaging while they viewed the faces of two unfamiliar individuals: one individual's face was partially accompanied by negative emotion but the other's was not. After the volunteers learned the relationship between the faces and the emotion, they formed a more negative impression of the person's face when the emotion was presented. Subtraction analysis of the individuals' neutral faces revealed activation in the dorsal anterior cingulate cortex and superior temporal sulcus, but this activity did not correlate with the change of impression from face. On the other hand, the response in the left amygdala negatively correlated with the change of impression from face in the first run. Time modulation analysis revealed that activity in the dorsomedial prefrontal cortex associated with negative emotion was the largest in the initial part of the acquisition. These results suggest that a negative impression from face may be formed by orchestrated activity in the dorsomedial prefrontal cortex, dorsal anterior cingulate cortex and amygdala, and that the activity has a prominent role in the initial acquisition of negative emotion.

  19. Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase.

    PubMed

    Zimoch-Korzycka, Anna; Bobak, Łukasz; Jarmoluk, Andrzej

    2016-01-01

    The aim of this study was to evaluate the impact of cellulase (C) on the biological activity of chitosan/hydroxypropyl methylcellulose (CH/HPMC) film-forming hydrosols. The hydrolytic activity of cellulase in two concentrations (0.05% and 0.1%) was verified by determination of the progress of polysaccharide hydrolysis, based on viscosity measurement and reducing sugar-ends assay. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging effect, the ferric reducing antioxidant power (FRAP), and microbial reduction of Pseudomonas fluorescens, Yersinia enterocolitica, Bacillus cereus, and Staphylococcus aureus were studied. During the first 3 h of reaction, relative reducing sugar concentration increased progressively, and viscosity decreased rapidly. With increasing amount of enzyme from 0.05% to 0.1%, the reducing sugar concentration increased, and the viscosity decreased significantly. The scavenging effect of film-forming solutions was improved from 7.6% at time 0 and without enzyme to 52.1% for 0.1% cellulase after 20 h of reaction. A significant effect of cellulase addition and reaction time on antioxidant power of the tested film-forming solutions was also reported. Film-forming hydrosols with cellulase exhibited a bacteriostatic effect on all tested bacteria, causing a total reduction.

  20. Channel-forming activities of peroxisomal membrane proteins from the yeast Saccharomyces cerevisiae.

    PubMed

    Grunau, Silke; Mindthoff, Sabrina; Rottensteiner, Hanspeter; Sormunen, Raija T; Hiltunen, J Kalervo; Erdmann, Ralf; Antonenkov, Vasily D

    2009-03-01

    Highly-purified peroxisomes from the yeast Saccharomyces cerevisiae grown on oleic acid were investigated for the presence of channel (pore)-forming proteins in the membrane of these organelles. Solubilized membrane proteins were reconstituted in planar lipid bilayers and their pore-forming activity was studied by means of multiple-channel monitoring or single-channel analysis. Two abundant pore-forming activities were detected with an average conductance of 0.2 and 0.6 nS in 1.0 m KCl, respectively. The high-conductance pore (0.6 nS in 1.0 m KCl) is slightly selective to cations (P(K+)/P(Cl-) approximately 1.3) and showed an unusual flickering at elevated (> +/-40 mV) holding potentials directed upward relative to the open state of the channel. The data obtained for the properties of the low-conductance pore (0.2 nS in 1.0 m KCl) support the notion that the high-conductance channel represents a cluster of two low-conductance pores. The results lead to conclusion that the yeast peroxisomes contain membrane pore-forming proteins that may aid the transfer of small solutes between the peroxisomal lumen and cytoplasm.

  1. Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase

    PubMed Central

    Zimoch-Korzycka, Anna; Bobak, Łukasz; Jarmoluk, Andrzej

    2016-01-01

    The aim of this study was to evaluate the impact of cellulase (C) on the biological activity of chitosan/hydroxypropyl methylcellulose (CH/HPMC) film-forming hydrosols. The hydrolytic activity of cellulase in two concentrations (0.05% and 0.1%) was verified by determination of the progress of polysaccharide hydrolysis, based on viscosity measurement and reducing sugar-ends assay. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging effect, the ferric reducing antioxidant power (FRAP), and microbial reduction of Pseudomonas fluorescens, Yersinia enterocolitica, Bacillus cereus, and Staphylococcus aureus were studied. During the first 3 h of reaction, relative reducing sugar concentration increased progressively, and viscosity decreased rapidly. With increasing amount of enzyme from 0.05% to 0.1%, the reducing sugar concentration increased, and the viscosity decreased significantly. The scavenging effect of film-forming solutions was improved from 7.6% at time 0 and without enzyme to 52.1% for 0.1% cellulase after 20 h of reaction. A significant effect of cellulase addition and reaction time on antioxidant power of the tested film-forming solutions was also reported. Film-forming hydrosols with cellulase exhibited a bacteriostatic effect on all tested bacteria, causing a total reduction. PMID:27608008

  2. Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase.

    PubMed

    Zimoch-Korzycka, Anna; Bobak, Łukasz; Jarmoluk, Andrzej

    2016-01-01

    The aim of this study was to evaluate the impact of cellulase (C) on the biological activity of chitosan/hydroxypropyl methylcellulose (CH/HPMC) film-forming hydrosols. The hydrolytic activity of cellulase in two concentrations (0.05% and 0.1%) was verified by determination of the progress of polysaccharide hydrolysis, based on viscosity measurement and reducing sugar-ends assay. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging effect, the ferric reducing antioxidant power (FRAP), and microbial reduction of Pseudomonas fluorescens, Yersinia enterocolitica, Bacillus cereus, and Staphylococcus aureus were studied. During the first 3 h of reaction, relative reducing sugar concentration increased progressively, and viscosity decreased rapidly. With increasing amount of enzyme from 0.05% to 0.1%, the reducing sugar concentration increased, and the viscosity decreased significantly. The scavenging effect of film-forming solutions was improved from 7.6% at time 0 and without enzyme to 52.1% for 0.1% cellulase after 20 h of reaction. A significant effect of cellulase addition and reaction time on antioxidant power of the tested film-forming solutions was also reported. Film-forming hydrosols with cellulase exhibited a bacteriostatic effect on all tested bacteria, causing a total reduction. PMID:27608008

  3. Validity and Reliability of International Physical Activity Questionnaire-Short Form in Chinese Youth

    ERIC Educational Resources Information Center

    Wang, Chao; Chen, Peijie; Zhuang, Jie

    2013-01-01

    Purpose: The psychometric profiles of the widely used International Physical Activity Questionnaire-Short Form (IPAQ-SF) in Chinese youth have not been reported. The purpose of this study was to examine the validity and reliability of the IPAQ-SF using a sample of Chinese youth. Method: One thousand and twenty-one youth (M[subscript age] = 14.26 ±…

  4. Unique SMAD1/5/8 activity at the phalanx-forming region determines digit identity

    PubMed Central

    Suzuki, Takayuki; Hasso, Sean M.; Fallon, John F.

    2008-01-01

    The zone of polarizing activity is the primary signaling center controlling anterior–posterior patterning of the amniote limb bud. The autopodial interdigits (IDs) are secondary signaling centers proposed to determine digit identity by acting on the cells of the digital ray. Here, we focus on events accompanying digital fate determination and define a region of the digital ray that expresses Sox9 and Bmpr1b and is phosphorylated-SMAD1/5/8 (p-SMAD1/5/8) positive. We name this region the phalanx-forming region (PFR), and show that the PFR cells arise from the distal subridge mesenchyme of digital ray. This phalanx-forming cell lineage is subsequently committed to the cartilage lineage; the fate of these cells is initially labile but becomes fixed as they are incorporated into the condensed cartilage of the digit primordium. Using an in vivo reporter assay, we establish that each digital PFR has a unique p-SMAD1/5/8 activity signature. In addition, we show that changes in this activity correlate with the identity of the digit that forms after experimental manipulation, supporting the idea that threshold signaling levels can lead to different developmental outcomes in a morphogenetic field. Our data define the molecular profile of the PFR, and we propose a model for understanding formation and variation of digits during autopodial development. PMID:18334652

  5. Nurse eggs form through an active process of apoptosis in the spionid Polydora cornuta (Annelida).

    PubMed

    Gibson, Glenys; Hart, Corban; Coulter, Claire; Xu, Haixin

    2012-07-01

    The production of nurse eggs is fundamental to poecilogony in some species of spionid annelids. In species such as Polydora cornuta, nurse-egg production varies among females and ingestion of nurse eggs varies among young, resulting in a form of poecilogony with divergent phenotypes for females (e.g., fecundity and per-offspring investment) as well as for larvae (e.g., trophic mode, size, and stage at hatching). We tested the hypothesis that nurse eggs of P. cornuta form through an active developmental process and specifically, through apoptosis. Results of a TUNEL assay indicate nuclear fragmentation occurs in a process that is characteristic of apoptosis. Cellular indicators of apoptosis in nurse eggs include activation of caspase-3, a positive Annexin V reaction indicating exposure of phosphatidylserine on the outer cell membrane, and invagination of the membrane to form yolk vesicles. These results indicate that formation of nurse eggs in this population of P. cornuta occurs through an active, adaptive process. Furthermore, while apoptosis also occurs in some cells of P. cornuta embryos, it was not detected until later in development. This suggests that nurse eggs originate through heterochrony in a developmental process (apoptosis) that is common to all young of P. cornuta.

  6. Mechanistic insights into the first Lygus-active β-pore forming protein.

    PubMed

    Jerga, Agoston; Chen, Danqi; Zhang, Chunfen; Fu, Jinping; Kouadio, Jean-Louis K; Wang, Yanfei; Duff, Stephen M G; Howard, Jennifer E; Rydel, Timothy J; Evdokimov, Artem G; Ramaseshadri, Parthasarathy; Evans, Adam; Bolognesi, Renata; Park, Yoonseong; Haas, Jeffrey A

    2016-06-15

    The cotton pests Lygus hesperus and Lygus lineolaris can be controlled by expressing Cry51Aa2.834_16 in cotton. Insecticidal activity of pore-forming proteins is generally associated with damage to the midgut epithelium due to pores, and their biological specificity results from a set of key determinants including proteolytic activation and receptor binding. We conducted mechanistic studies to gain insight into how the first Lygus-active β-pore forming protein variant functions. Biophysical characterization revealed that the full-length Cry51Aa2.834_16 was a stable dimer in solution, and when exposed to Lygus saliva or to trypsin, the protein underwent proteolytic cleavage at the C-terminus of each of the subunits, resulting in dissociation of the dimer to two separate monomers. The monomer showed tight binding to a specific protein in Lygus brush border membranes, and also formed a membrane-associated oligomeric complex both in vitro and in vivo. Chemically cross-linking the β-hairpin to the Cry51Aa2.834_16 body rendered the protein inactive, but still competent to compete for binding sites with the native protein in vivo. Our study suggests that disassociation of the Cry51Aa2.834_16 dimer into monomeric units with unoccupied head-region and sterically unhindered β-hairpin is required for brush border membrane binding, oligomerization, and the subsequent steps leading to insect mortality. PMID:27001423

  7. The Prodomain-bound Form of Bone Morphogenetic Protein 10 Is Biologically Active on Endothelial Cells*

    PubMed Central

    Jiang, He; Salmon, Richard M.; Upton, Paul D.; Wei, Zhenquan; Lawera, Aleksandra; Davenport, Anthony P.; Morrell, Nicholas W.; Li, Wei

    2016-01-01

    BMP10 is highly expressed in the developing heart and plays essential roles in cardiogenesis. BMP10 deletion in mice results in embryonic lethality because of impaired cardiac development. In adults, BMP10 expression is restricted to the right atrium, though ventricular hypertrophy is accompanied by increased BMP10 expression in a rat hypertension model. However, reports of BMP10 activity in the circulation are inconclusive. In particular, it is not known whether in vivo secreted BMP10 is active or whether additional factors are required to achieve its bioactivity. It has been shown that high-affinity binding of the BMP10 prodomain to the mature ligand inhibits BMP10 signaling activity in C2C12 cells, and it was proposed that prodomain-bound BMP10 (pBMP10) complex is latent. In this study, we demonstrated that the BMP10 prodomain did not inhibit BMP10 signaling activity in multiple endothelial cells, and that recombinant human pBMP10 complex, expressed in mammalian cells and purified under native conditions, was fully active. In addition, both BMP10 in human plasma and BMP10 secreted from the mouse right atrium were fully active. Finally, we confirmed that active BMP10 secreted from mouse right atrium was in the prodomain-bound form. Our data suggest that circulating BMP10 in adults is fully active and that the reported vascular quiescence function of BMP10 in vivo is due to the direct activity of pBMP10 and does not require an additional activation step. Moreover, being an active ligand, recombinant pBMP10 may have therapeutic potential as an endothelial-selective BMP ligand, in conditions characterized by loss of BMP9/10 signaling. PMID:26631724

  8. Cell surface activation of the erythropoietin receptor by Friend spleen focus-forming virus gp55.

    PubMed Central

    Li, J P; Hu, H O; Niu, Q T; Fang, C

    1995-01-01

    The leukemogenic membrane glycoprotein gp55, encoded by Friend spleen focus-forming virus (SFFV), induces erythroid cell proliferation through its interaction with the erythropoietin receptor (EPO-R). There are two forms of gp55 in SFFV-infected cells: an intracellular form (more than 95% of the total protein), which is localized within the endoplasmic reticulum (ER) membranes, and a cell surface form (about 3 to 5%). Because both forms of the viral proteins bind to EPO-R, it is not clear whether the viral protein induces mitogenesis intracellularly or at the cell surface. To address this question, we constructed an EPO-R mutant that contained a 6-amino-acid (DEKKMP) C-terminus ER retention signal. Biochemical and functional analyses with this mutant indicated that it was completely retained in the ER and not expressed at the cell surface. Further analysis showed that the mutant, like the wild-type EPO-R, interacted with SFFV gp55. However, this apparent intracellular interaction between the two proteins failed to induce growth factor-independent proliferation of Ba/F3 cells. Furthermore, spontaneous variants of the ER-retained EPO-R selected on the basis of their ability to induce cell proliferation when coexpressed with gp55 were exclusively expressed at the cell surface. Thus, our results support the hypothesis that the mitogenic activation of the EPO-R by gp55 requires the interaction of the two proteins at the cell surface. PMID:7853508

  9. Are state renewable feed-in tariff initiatives truly throttled by Federal statutes after the FERC California decision?

    SciTech Connect

    Yaffe, David P.

    2010-10-15

    For the last few years, several local and state governments have adopted ''feed-in tariffs'' to promote development of dispersed, small-scale renewable generation through incentive pricing. Most FITs are intended to stimulate development of small solar or renewable energy facilities. In July, FERC issued a decision restating that the Federal Power Act and PURPA 210, not state (or local) legislation, govern the price that local utilities may pay under FITs. (author)

  10. Brain activation patterns resulting from learning letter forms through active self-production and passive observation in young children

    PubMed Central

    Kersey, Alyssa J.; James, Karin H.

    2013-01-01

    Although previous literature suggests that writing practice facilitates neural specialization for letters, it is unclear if this facilitation is driven by the perceptual feedback from the act of writing or the actual execution of the motor act. The present study addresses this issue by measuring the change in BOLD signal in response to hand-printed letters, unlearned cursive letters, and cursive letters that 7-year-old children learned actively, by writing, and passively, by observing an experimenter write. Brain activation was assessed using fMRI while perceiving letters—in both cursive and manuscript forms. Results showed that active training led to increased recruitment of the sensori-motor network associated with letter perception as well as the insula and claustrum, but passive observation did not. This suggests that perceptual networks for newly learned cursive letters are driven by motor execution rather than by perceptual feedback. PMID:24069007

  11. Brain activation patterns resulting from learning letter forms through active self-production and passive observation in young children.

    PubMed

    Kersey, Alyssa J; James, Karin H

    2013-01-01

    Although previous literature suggests that writing practice facilitates neural specialization for letters, it is unclear if this facilitation is driven by the perceptual feedback from the act of writing or the actual execution of the motor act. The present study addresses this issue by measuring the change in BOLD signal in response to hand-printed letters, unlearned cursive letters, and cursive letters that 7-year-old children learned actively, by writing, and passively, by observing an experimenter write. Brain activation was assessed using fMRI while perceiving letters-in both cursive and manuscript forms. Results showed that active training led to increased recruitment of the sensori-motor network associated with letter perception as well as the insula and claustrum, but passive observation did not. This suggests that perceptual networks for newly learned cursive letters are driven by motor execution rather than by perceptual feedback.

  12. The effects of two forms of physical activity on eyeblink classical conditioning.

    PubMed

    Green, John T; Chess, Amy C; Burns, Montana; Schachinger, Kira M; Thanellou, Alexandra

    2011-05-16

    Voluntary exercise, in the form of free access to a running wheel in the home cage, has been shown to improve several forms of learning and memory. Acrobatic training, in the form of learning to traverse an elevated obstacle course, has been shown to induce markers of neural plasticity in the cerebellar cortex in rodents. In three experiments, we examined the effects of these two forms of physical activity on delay eyeblink conditioning in rats. In Experiment 1, exercising rats were given 17 days of free access to a running wheel in their home cage prior to 10 days of delay eyeblink conditioning. Rats that exercised conditioned significantly better and showed a larger reflexive eyeblink unconditioned response to the periocular stimulation unconditioned stimulus than rats that did not exercise. In Experiment 2, exercising rats were given 17 days of free access to a running wheel in their home cage prior to 10 days of explicitly unpaired stimulus presentations. Rats that exercised responded the same to tone, light, and periocular stimulation as rats that did not exercise. In Experiment 3, acrobatic training rats were given 15 days of daily training on an elevated obstacle course prior to 10 days of eyeblink conditioning. Activity control rats underwent 15 days of yoked daily running in an open field. Rats that underwent acrobatic training did not differ in eyeblink conditioning from activity control rats. The ability to measure the learned response precisely, and the well-mapped neural circuitry of eyeblink conditioning offer some advantages for the study of exercise effects on learning and memory.

  13. Neuroserpin Differentiates Between Forms of Tissue Type Plasminogen Activator via pH Dependent Deacylation

    PubMed Central

    Carlson, Karen-Sue B.; Nguyen, Lan; Schwartz, Kat; Lawrence, Daniel A.; Schwartz, Bradford S.

    2016-01-01

    Tissue-type plasminogen activator (t-PA), initially characterized for its critical role in fibrinolysis, also has key functions in both physiologic and pathologic processes in the CNS. Neuroserpin (NSP) is a t-PA specific serine protease inhibitor (serpin) found almost exclusively in the CNS that regulates t-PA’s proteolytic activity and protects against t-PA mediated seizure propagation and blood–brain barrier disruption. This report demonstrates that NSP inhibition of t-PA varies profoundly as a function of pH within the biologically relevant pH range for the CNS, and reflects the stability, rather than the formation of NSP: t-PA acyl-enzyme complexes. Moreover, NSP differentiates between the zymogen-like single chain form (single chain t-PA, sct-PA) and the mature protease form (two chain t-PA, tct-PA) of t-PA, demonstrating different pH profiles for protease inhibition, different pH ranges over which catalytic deacylation occurs, and different pH dependent profiles of deacylation rates for each form of t-PA. NSP’s pH dependent inhibition of t-PA is not accounted for by differential acylation, and is specific for the NSP-t-PA serpin-protease pair. These results demonstrate a novel mechanism for the differential regulation of the two forms of t-PA in the CNS, and suggest a potential specific regulatory role for CNS pH in controlling t-PA proteolytic activity. PMID:27378851

  14. Multifunctional porous titanium oxide coating with apatite forming ability and photocatalytic activity on a titanium substrate formed by plasma electrolytic oxidation.

    PubMed

    Akatsu, T; Yamada, Y; Hoshikawa, Y; Onoki, T; Shinoda, Y; Wakai, F

    2013-12-01

    Plasma electrolytic oxidation (PEO) was used to make a multifunctional porous titanium oxide (TiO2) coating on a titanium substrate. The key finding of this study is that a highly crystalline TiO2 coating can be made by performing the PEO in an ammonium acetate (CH3COONH4) solution; the PEO coating was formed by alternating between rapid heating by spark discharges and quenching in the solution. The high crystallinity of the TiO2 led to the surface having multiple functions, including apatite forming ability and photocatalytic activity. Hydroxyapatite formed on the PEO coating when it was soaked in simulated body fluid. The good apatite forming ability can be attributed to the high density of hydroxyl groups on the anatase and rutile phases in the coating. The degradation of methylene blue under ultraviolet radiation indicated that the coating had high photocatalytic activity. PMID:24094199

  15. Multifunctional porous titanium oxide coating with apatite forming ability and photocatalytic activity on a titanium substrate formed by plasma electrolytic oxidation.

    PubMed

    Akatsu, T; Yamada, Y; Hoshikawa, Y; Onoki, T; Shinoda, Y; Wakai, F

    2013-12-01

    Plasma electrolytic oxidation (PEO) was used to make a multifunctional porous titanium oxide (TiO2) coating on a titanium substrate. The key finding of this study is that a highly crystalline TiO2 coating can be made by performing the PEO in an ammonium acetate (CH3COONH4) solution; the PEO coating was formed by alternating between rapid heating by spark discharges and quenching in the solution. The high crystallinity of the TiO2 led to the surface having multiple functions, including apatite forming ability and photocatalytic activity. Hydroxyapatite formed on the PEO coating when it was soaked in simulated body fluid. The good apatite forming ability can be attributed to the high density of hydroxyl groups on the anatase and rutile phases in the coating. The degradation of methylene blue under ultraviolet radiation indicated that the coating had high photocatalytic activity.

  16. 77 FR 58116 - Commission Information Collection Activities (FERC-732); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-19

    ... Rights in Organized Electricity Markets. DATES: Comments on the collection of information are due... Rights in Organized Electricity Markets. OMB Control No.:1902-0245. Type of Request: 18 CFR part 42... transmission organization that is a public utility with one or more organized electricity markets to...

  17. 77 FR 73630 - Commission Information Collection Activities (FERC-732); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ... explained below. The Commission issued a Notice in the Federal Register (77 FR 58116, 9/19/2012) requesting... and Tariffs: Long-Term Firm Transmission Rights in Organized Electricity Markets) to the Office of... Tariffs: Long-Term Firm Transmission Rights in Organized Electricity Markets. OMB Control No.:...

  18. 76 FR 31320 - Commission Information Collection Activities (FERC-725B); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... that have identified Critical Cyber Assets. Category 2--Estimate of U.S. 1,156 1 120 138,720 Entities that have not identified Critical Cyber Assets. Category 3--New U.S. Entities 6 1 3,840 23,040 that... compliance with the requirements of section 3507 of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507,...

  19. 76 FR 19333 - Commission Information Collection Activities (FERC-725B); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-07

    ... identified Critical Cyber Assets Estimate of U.S. Entities that have not 1,156 1 8 9,248 identified Critical Cyber Assets New U.S. Entities that have to come into *6 1 1,176 7,056 compliance with the CIP Standards... compliance with the requirements of section 3507 of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507,...

  20. 76 FR 18743 - Commission Information Collection Activities (FERC-603); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    ... Information Act (5 USC 552). On February, 21, 2003, the Commission issued Order No. 630 (66 FR 52917) to... assets, whether physical or virtual, the incapacity or destruction of which would negatively affect... access to CEII may file a request for that information by providing information about their identity...

  1. 76 FR 55370 - Commission Information Collection Activities (FERC-549B); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ... remedy anti-competitive behavior. Order 637 required a pipeline post the information quarterly on its... Federal Register notice of June 23, 2011 (76 FR 36909) and has made this notation in its submission to OMB... availability and price data that shippers needed to make informed decisions in a competitive market as well...

  2. 76 FR 36909 - Commission Information Collection Activities (FERC-549B); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-23

    ... that shippers need to make informed decisions in a competitive market as well as improve shippers' and the Commission's ability to monitor marketplace behavior to detect, and remedy anti-competitive... needed to make informed decisions in a competitive market as well as to improve shipper's and...

  3. 78 FR 29359 - Commission Information Collection Activities (Ferc-604); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-20

    ... Respondents: Public utilities, natural gas companies, and oil pipeline companies. Estimate of Annual Burden \\1... than $1 billion) between regulated pipeline affiliates and non-regulated parents whose financial...) = (C) (D) (C) x (D) Public utilities and licensees, natural gas companies, and oil \\2\\ 25 1 25 1.5...

  4. 76 FR 28014 - Commission Information Collection Activities (FERC-600); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    ... charged by oil pipeline companies subject to its jurisdiction. If a proposed oil rate has been filed and.... The data in complaints filed by interested/affected parties regarding oil and natural gas pipeline... the requirements of subsection (f) as provided in subparagraph (A) of this paragraph. Likewise for...

  5. 75 FR 74696 - Commission Information Collection Activities (FERC-555); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-01

    ... for retention of applicable records for the regulated public utilities, natural gas and oil pipeline... issued a Notice in the Federal Register (75 FR 57744, 09/22/2010) requesting public comments....

  6. 78 FR 61983 - Commission Information Collection Activities (FERC-555); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-09

    ... apply to the regulated public utilities, natural gas and oil pipeline companies subject to the... regulations for public utilities and licensees as well as natural gas and oil pipeline companies. These..., Natural Gas and Oil Pipeline Companies Number of Average burden Number of respondents responses per...

  7. 76 FR 45237 - Commission Information Collection Activities (FERC-600); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... oil pipelines, Part 1 of the Interstate Commerce Act (ICA), sections 1, 6 and 15 (re-codified by Pub... the rates charged by oil pipeline companies subject to its jurisdiction. If a proposed oil rate has.../affected parties regarding oil and natural gas pipeline operations, electric and hydropower facilities...

  8. 76 FR 12090 - Commission Information Collection Activities (FERC-73); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    ... Energy Organization Act, 42 U.S.C. 7155 and 7172, and Executive Order No. 12009, 42 FR 46277 (September.... The Commission implements these filings in 18 CFR parts 347 and 357. Action: The Commission...

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    Federal Register 2010, 2011, 2012, 2013, 2014

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    ... Pipelines. This collection originally affected both intrastate and interstate natural gas pipelines, but for reasons described below only pertains to interstate natural gas pipelines. DATES: Comments on the... information collection requirements with no changes to the current reporting requirements except as...

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    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-22

    ... where none of Independent Power Producer the costs were recovered through an (IPP) provide the... for each reporting year 2012 and 2013. Do not report purchased power where none of the costs were... columns and either ``Only energy charges'' or ``The total cost of the purchase of economic power''...

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    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-22

    ... attributable to providing this information, such as administrative costs and the cost for information...) (2) (3) (1)x(2)x(3) 16 1 10 160 ] Estimated cost burden to respondents is $10,952 (160 hours/2080... cost per respondent is $685 (rounded). The reporting burden includes the total time, effort,...

  12. 76 FR 34689 - Commission Information Collection Activities [FERC-583], Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-14

    ... government administrative costs and for the use of government dams. The Commission implements these filing... attributable to providing this information, such as ] administrative costs and the cost for information... hydropower licensees for, among other things, the cost of administering part I of the FPA and for the use...

  13. 76 FR 34216 - Commission Information Collection Activities (Ferc-521), Proposed Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-13

    ..., ``Payments for Benefits from Headwater Benefits'' (OMB No. 1902-0087), is used by the Commission to implement... authorizes the Commission to determine headwater benefits received by downstream hydropower project owners. Headwater benefits is the additional energy production possible at a downstream hydropower project...

  14. Special Form Testing of Sealed Source Encapsulation for High-Alpha-Activity Actinide Materials

    SciTech Connect

    Martinez, Oscar A

    2016-01-01

    In the United States all transportation of radioactive material is regulated by the U.S. Department of Transportation (DOT). Beginning in 2008 a new type of sealed-source encapsulation package was developed and tested by Oak Ridge National Laboratory (ORNL). These packages contain high-alpha-activity actinides and are regulated and transported in accordance with the requirements for DOT Class 7 hazardous material. The DOT provides specific regulations pertaining to special form encapsulation designs. The special form designation indicates that the encapsulated radioactive contents have a very low probability of dispersion even when subjected to significant structural events. The special form designs have been shown to simplify the delivery, transport, acceptance, and receipt processes. It is intended for these sealed-source encapsulations to be shipped to various facilities making it very advantageous for them to be certified as special form. To this end, DOT Certificates of Competent Authority (CoCAs) have been sought for the design suitable for containing high-alpha-activity actinide materials. This design consists of the high-alpha-activity material encapsulated within a triangular zirconia canister, referred to as a ZipCan, tile that is then enclosed by a spherical shell. The spherical shell design, with ZipCan tile inside, was tested for compliance with the special form regulations found in 49 CFR 173.469. The spherical enclosure was subjected to 9-m impact, 1 m percussion, and 10-minute thermal tests at the Packaging Evaluation Facility located at the National Transportation Research Center in Knoxville, TN USA and operated by ORNL. Before and after each test, the test units were subjected to a helium leak check and a bubble test. The ZipCan tiles and core were also subjected to the tests required for ISO 2919:2012(E), including a Class IV impact test and heat test and subsequently subjected to helium leakage rate tests [49 CFR 173.469(a)(4)(i)]. The impact

  15. Staphylococcus aureus forms spreading dendrites that have characteristics of active motility.

    PubMed

    Pollitt, Eric J G; Crusz, Shanika A; Diggle, Stephen P

    2015-01-01

    Staphylococcus aureus is historically regarded as a non-motile organism. More recently it has been shown that S. aureus can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites: branching structures that emerge from the central colony. We discovered that S. aureus can spread across the surface of media in structures that we term 'comets', which advance outwards and precede the formation of dendrites. We observed comets in a diverse selection of S. aureus isolates and they exhibit the following behaviours: (1) They consist of phenotypically distinct cores of cells that move forward and seed other S. aureus cells behind them forming a comet 'tail'; (2) they move when other cells in the comet tail have stopped moving; (3) the comet core is held together by a matrix of slime; and (4) the comets etch trails in the agar as they move forwards. Comets are not consistent with spreading motility or other forms of passive motility. Comet behaviour does share many similarities with a form of active motility known as gliding. Our observations therefore suggest that S. aureus is actively motile under certain conditions. PMID:26680153

  16. Staphylococcus aureus forms spreading dendrites that have characteristics of active motility

    PubMed Central

    Pollitt, Eric J. G.; Crusz, Shanika A.; Diggle, Stephen P.

    2015-01-01

    Staphylococcus aureus is historically regarded as a non-motile organism. More recently it has been shown that S. aureus can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites: branching structures that emerge from the central colony. We discovered that S. aureus can spread across the surface of media in structures that we term ‘comets’, which advance outwards and precede the formation of dendrites. We observed comets in a diverse selection of S. aureus isolates and they exhibit the following behaviours: (1) They consist of phenotypically distinct cores of cells that move forward and seed other S. aureus cells behind them forming a comet ‘tail’; (2) they move when other cells in the comet tail have stopped moving; (3) the comet core is held together by a matrix of slime; and (4) the comets etch trails in the agar as they move forwards. Comets are not consistent with spreading motility or other forms of passive motility. Comet behaviour does share many similarities with a form of active motility known as gliding. Our observations therefore suggest that S. aureus is actively motile under certain conditions. PMID:26680153

  17. An Aqueous Extract of Marine Microalgae Exhibits Antimetastatic Activity through Preferential Killing of Suspended Cancer Cells and Anticolony Forming Activity

    PubMed Central

    Somasekharan, Syam Prakash; El-Naggar, Amal; Sorensen, Poul H.

    2016-01-01

    Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma. In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells. In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted.

  18. An Aqueous Extract of Marine Microalgae Exhibits Antimetastatic Activity through Preferential Killing of Suspended Cancer Cells and Anticolony Forming Activity

    PubMed Central

    Somasekharan, Syam Prakash; El-Naggar, Amal; Sorensen, Poul H.

    2016-01-01

    Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma. In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells. In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted. PMID:27656243

  19. An Aqueous Extract of Marine Microalgae Exhibits Antimetastatic Activity through Preferential Killing of Suspended Cancer Cells and Anticolony Forming Activity.

    PubMed

    Somasekharan, Syam Prakash; El-Naggar, Amal; Sorensen, Poul H; Wang, Yuzhuo; Cheng, Hongwei

    2016-01-01

    Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma. In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells. In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted. PMID:27656243

  20. In vitro activity of N-benzenesulfonylbenzotriazole on Trypanosoma cruzi epimastigote and trypomastigote forms.

    PubMed

    Becerra, M C; Guiñazú, N; Hergert, L Y; Pellegrini, A; Mazzieri, M R; Gea, S; Albesa, I

    2012-05-01

    Chagas disease is still an important health problem in Central and South America. However, the only drugs currently available for specific treatment of this disease may induce toxic side effects in the host. The aim of this work was to determine the activity of N-benzenesulfonylbenzotriazole (BSBZT) against the protozoan parasite Trypanosoma cruzi. The effects of BSBZT and benzotriazole (BZT) were compared to those of benznidazole (BZL) on epimastigote and trypomastigote forms. BSBZT was found to have an in vitro growth inhibitory dose-dependent activity against epimastigotes, with flow cytometry analysis confirming that the treated parasites presented size reduction. BSBZT showed an IC(50) of 21.56 μg/mL (81.07 μM) against epimastigotes at 72 h of incubation, whereas BZT did not affect the growth of this parasite form. Furthermore, the toxic effect of BSBZT, was stronger and appeared earlier (at 24h) in trypomastigotes than in epimastigotes, with the LC(50) of this compound being 28.40 μg/mL (106.79 μM) against trypomastigotes. The concentrations of BSBZT used in this study presented low hemolytic activity and cytotoxicity. Consequently, at concentrations near IC(50) and LC(50) (25μg/mL), BSBZT caused only 2.4% hemolysis and 15% of RAW 264.7 cell cytotoxicity. These results reveal the potential of BSBZT as a prototype in drug design for developing new anti-T. cruzi compounds.

  1. Hypo-activity screening in school setting; examining reliability and validity of the Teacher Estimation of Activity Form (TEAF).

    PubMed

    Rosenblum, Sara; Engel-Yeger, Batya

    2015-06-01

    It is well established that physical activity during childhood contributes to children's physical and psychological health. The aim of this study was to test the reliability and validity of the Hebrew version of the Teacher Estimation of Activity Form (TEAF) questionnaire as a screening tool among school-aged children in Israel. Six physical education teachers completed TEAF questionnaires of 123 children aged 5-12 years, 68 children (55%) with Typical Development (TD) and 55 children (45%) diagnosed with Developmental Coordination Disorder (DCD). The Hebrew version of the TEAF indicates a very high level of internal consistency (α = .97). There were no significant gender differences. Significant differences were found between children with and without DCD attesting to the test's construct validity. Concurrent validity was established by finding a significant high correlation (r = .76, p < .01) between the TEAF and the Movement-ABC mean scores within the DCD group. The TEAF demonstrated acceptable reliability and validity estimates. It appears to be a promising standardized practical tool in both research and practice for describing information about school-aged children's involvement in physical activity. Further research is indicated with larger samples to establish cut-off scores determining what point identifies hypo activity in striated age groups. Furthermore, the majority of the participants in this study were boys, and further research is needed to include more girls for a better understanding of the phenomena of hypo activity.

  2. Glutathione peroxidase activity and chemical forms of selenium in tissues of rats given selenite or selenomethionine

    SciTech Connect

    Beilstein, M.A.; Whanger, P.D.

    1988-05-01

    Glutathione peroxidase (GPx) activity and deposition of selenium (Se) were examined in tissues of rats given dietary Se for 7 wk as either selenite or selenomethionine (SeMet) with 75Se radiotracer of the same chemical form. On the basis of Se:75Se ratio, all tissues of the rats fed selenite were equilibrated with the dietary source, but tissues of the SeMet fed animals maintained a ratio of Se:75Se greater than the dietary ratio. Deposition of dietary Se and 75Se was higher in most tissues of rats fed SeMet. Muscle 75Se was the largest single tissue pool of 75Se in both groups accounting for one-third of recovered 75Se in the rats fed selenite, and one-half of recovered 75Se in the rats fed SeMet. Tissue GPx activities were not different between the two dietary groups. The proportion of Se as GPx in tissues was highest in erythrocytes of the rats fed selenite (.81) and lowest in testes and epididymides of the rats fed SeMet (.009). The proportion of Se present in cytosolic GPx was consistently higher in tissues of rats fed selenite. Erythrocytes of the rats fed SeMet had more 75Se associated with hemoglobin, and muscle cytosols of the rats fed selenite had more 75Se associated with the G-protein. The proportion of 75Se as SeMet determined by ion exchange chromatography of tissue hydrolysates was higher in tissues of rats fed SeMet (highest in muscle and hemoglobin, 70%, and lowest in testes, 16%). In contrast, selenocysteine was the predominant form of Se present in tissues of rats given selenite. These results indicate that the form of Se administered will influence the form in the tissues, the percentage of Se with GPx and the body burden of Se.

  3. Isolation of a biologically active soluble form of the hemagglutinin-neuraminidase protein of Sendai virus.

    PubMed Central

    Thompson, S D; Laver, W G; Murti, K G; Portner, A

    1988-01-01

    As a first step in establishing the three-dimensional structure of the Sendai virus hemagglutinin-neuraminidase (HN), we have isolated and characterized a potentially crystallizable form of the molecule. The sequence of HN, a surface glycoprotein, predicts a protein with an uncharged hydrophobic region near the amino terminus which is responsible for anchorage in the viral envelope. To avoid rosette formation (aggregation), which would preclude crystallization, this hydrophobic tail was removed from a membrane-free form of HN by proteolytic digestion. This digestion resulted in a single product with a molecular weight of about 10,000 less than native HN. N-terminal amino acid sequence analysis of cleaved HN (C-HN) indicated a single cleavage site at amino acid residue 131, resulting in a product consisting of the carboxyl-terminal 444 amino acids of HN. Functional analyses revealed that C-HN retained full neuraminidase activity and was able to bind erythrocytes, indicating that the N-terminal 131 residues were not necessary for these biological activities. Furthermore, this cleavage product retained the antigenic structure of intact HN, since monoclonal antibodies still bound to C-HN in enzyme-linked immunosorbent assay and Western (immuno-) blot analysis. Viewed by electron microscopy, the dimeric and tetrameric forms of intact HN form rosettes while C-HN maintains the oligomeric structure but no longer aggregates. Furthermore, the electron micrographs revealed a C-HN tetramer strikingly similar to the influenza virus neuraminidase in both size and gross structural features. Images PMID:2846877

  4. 18 CFR 131.80 - FERC Form No. 556, Certification of qualifying facility status for an existing or a proposed...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... (Section 292.202(k)), solar, geothermal, wind, waste, biomass (Section 292.202(a)), or other). For a waste... public. 8. If the facility reported herein is not an eligible solar, wind, waste or geothermal facility...): An eligible solar, wind, waste or geothermal facility, as defined in Section 3(17)(E) of the...

  5. [Study of the Sporothrix schenkii (yeast forms) extract. Electrophoretic and immunoelectrophoretic analyses: characterization of enzymatic activities].

    PubMed

    Walbaum, S; Duriez, T; Dujardin, L; Biguet, J

    1978-07-28

    An extract from living yeast forms of S. schenckii was prepared. The yeasts originated from a shake culture in B.H.I. broth (Difco) incubated for 3 days at 35 degrees C in darkness; they were harvested, washed and disrupted with glass beads in a model MSK Braun mechanical cell homogenizer; a freezing-thawing was added to improve the extract. After electrophoretic separation in agarose gel, the extract's components were characterized by their enzymic activity; with this technique, 30 bands were revealed. These enzymic activities were also investigated on the antigenic fractions of the extract revealed by a rabbit hyperimmunserum: 16 among 22 immunoprecipitates are identified by their catalytic properties. Study of the earliest precipitating antibodies (appearing-order and enzymic caracterization) in rabbits just immunized completes this work. How to ameliorate the quality of the extract by culture and extraction conditions is also specified. PMID:692628

  6. The TCP1γ subunit of Leishmania donovani forms a biologically active homo-oligomeric complex.

    PubMed

    Bhaskar; Mitra, Kalyan; Kuldeep, Jitendra; Siddiqi, Mohammad Imran; Goyal, Neena

    2015-12-01

    Chaperonins are a class of molecular chaperons that encapsulate nascent or stress-denatured proteins and assist their intracellular assembly and folding in an ATP-dependent manner. The ubiquitous eukaryotic chaperonin, TCP1 ring complex is a hetero-oligomeric complex comprising two rings, each formed of eight subunits that may have distinct substrate recognition and ATP hydrolysis properties. In Leishmania, only the TCP1γ subunit has been cloned and characterized. It exhibited differential expression at various growth stages of promastigotes. In the present study, we expressed the TCP1γ subunit in Escherichia coli to investigate whether it forms chaperonin-like complexes and plays a role in protein folding. LdTCP1γ formed high-molecular-weight complexes within E. coli cells as well as in Leishmania cell lysates. The recombinant protein is arranged into two back-to-back rings of seven subunits each, as predicted by homology modelling and observed by negative staining electron microscopy. This morphology is consistent with that of the oligomeric double-ring group I chaperonins found in mitochondria. The LdTCP1γ homo-oligomeric complex hydrolysed ATP, and was active as assayed by luciferase refolding. Thus, the homo-oligomer performs chaperonin reactions without partner subunit(s). Further, co-immunoprecipitation studies revealed that LdTCP1γ interacts with actin and tubulin proteins, suggesting that the complex may have a role in maintaining the structural dynamics of the cytoskeleton of parasites. PMID:26395202

  7. Pore-forming activity and structural autoinhibition of the gasdermin family.

    PubMed

    Ding, Jingjin; Wang, Kun; Liu, Wang; She, Yang; Sun, Qi; Shi, Jianjin; Sun, Hanzi; Wang, Da-Cheng; Shao, Feng

    2016-07-01

    Inflammatory caspases cleave the gasdermin D (GSDMD) protein to trigger pyroptosis, a lytic form of cell death that is crucial for immune defences and diseases. GSDMD contains a functionally important gasdermin-N domain that is shared in the gasdermin family. The functional mechanism of action of gasdermin proteins is unknown. Here we show that the gasdermin-N domains of the gasdermin proteins GSDMD, GSDMA3 and GSDMA can bind membrane lipids, phosphoinositides and cardiolipin, and exhibit membrane-disrupting cytotoxicity in mammalian cells and artificially transformed bacteria. Gasdermin-N moved to the plasma membrane during pyroptosis. Purified gasdermin-N efficiently lysed phosphoinositide/cardiolipin-containing liposomes and formed pores on membranes made of artificial or natural phospholipid mixtures. Most gasdermin pores had an inner diameter of 10–14 nm and contained 16 symmetric protomers. The crystal structure of GSDMA3 showed an autoinhibited two-domain architecture that is conserved in the gasdermin family. Structure-guided mutagenesis demonstrated that the liposome-leakage and pore-forming activities of the gasdermin-N domain are required for pyroptosis. These findings reveal the mechanism for pyroptosis and provide insights into the roles of the gasdermin family in necrosis, immunity and diseases. PMID:27281216

  8. Theoretical study of the phototoxicity of naproxen and the active form of nabumetone.

    PubMed

    Musa, Klefah A K; Eriksson, Leif A

    2008-10-30

    Density functional theory using the hybrid functional B3LYP has been employed in order to study the mechanisms of photoinduced decomposition of the closely related nonsteroidal anti-inflammatory drugs naproxen (NP) and 6-methoxy-2-naphthylacetic acid (MNAA; the active form of nabumetone). The photochemical properties and computed energies of various species obtained in this study show that both drugs dominate in their deprotonated forms at physiological pH. The deprotonated acids are unable to decarboxylate from their excited singlets; instead, they decarboxylate from their first excited triplet states with high efficiency, overcoming energy barriers less than 3 and 1 kcal/mol for MNAA and NP, respectively. The ultraviolet and visible spectra of the neutral, deprotonated, and decarboxylated moieties of MNAA and NP are more-or-less similar but with higher probabilites (oscillator strength) for the latter. This fact, as well as the higher reactivity of NP, is explained in terms of the electron-donating effect of the additional methyl group present in NP. Singlet oxygen, superoxide radical anion, and corresponding peroxyl radical species are expected to be formed in different steps throughout the proposed photodegradation pathways of both drugs, which give rise to their effects on biomolecules, for example, lipid peroxidation.

  9. Role of activation in alveolar macrophage-mediated suppression of the plaque-forming cell response.

    PubMed Central

    Mbawuike, I N; Herscowitz, H B

    1988-01-01

    Alveolar macrophages (AM) are highly suppressive of the in vitro plaque-forming cell (PFC) response of spleen cells obtained from mice primed with sheep erythrocytes. Comparison of macrophage populations obtained from disparate anatomical sites revealed that although in both cases there was a cell-concentration-dependent suppression of the PFC response, resident AM or AM activated as a result of intravenous injection of Mycobacterium bovis BCG were equally suppressive at the doses examined. Although there was a similar dose-dependent suppression with peritoneal macrophages, BCG-activated cells were more suppressive of the PFC response than were resident cells. In contrast, splenic macrophages at comparable concentrations were not at all suppressive. Resident AM exhibited significantly lower levels of 5'-nucleotidase activity than did resident peritoneal macrophages. Macrophage-mediated suppression of the in vitro PFC response could not be attributed to the release of toxic oxygen metabolites (H2O2, O2- ,and .OH) or prostaglandins, since the addition of catalase, superoxide dismutase, 2-mercaptoethanol, or indomethacin did not completely reverse suppression. These results suggest that the lung microenvironment may maintain AM in an activated state which contributes to their potential immunoregulatory functions. PMID:2830191

  10. The active form of cytochrome c oxidase: effects of detergent, the intact membrane, and radiation inactivation

    SciTech Connect

    Thompson, D.A.; Suarez-Villafane, M.; Ferguson-Miller, S.

    1982-01-01

    Cytochrome oxidase is a multisubunit, intrinsic membrane protein with a complex function that includes oxidation of cytochrome c, reduction of oxygen and generation of a membrane potential. To clarify the relationship of its normal function to protein and membrane structure, we have examined the kinetic behavior of rat liver cytochrome oxidase in the intace inner mitochondrial membrane and in detergent solubilized states. Dissolution of rat liver mitochondrial membranes alters the kinetic parameters of the oxidase in a manner dependent in part on the dispersing agent, and characterized by a large increase in maximal activity which is not attributable to exposure of more oxidase or diminished affinity for cytochrome c. The most profound effect of solubilization of the membrane is seen on the low affinity reaction of cytochrome c, suggesting that the electron transfer pathway from this site to oxygen is sensitive to alterations in hydrophobic interactions within the oxidase. Purified rat liver and beef heart oxidase exists predominantly in a monodisperse, 300 kilodalton form in laurylmaltoside (Rosevear et al., 1980). However, a smaller, 130 kd species that exhibits high turnover rates equal to the 300 kd form is detected in some beef heart preparations, implying that the dimer may not be essential for high activity. Radiation inactivation studies on purified oxidase reveal a molecular weight for the functional unit of approx.70 kd. It is concluded that less than a complete set of subunits may be sufficient for both normal bindings of cytochrome c and rapid electron transfer to oxygen.

  11. Active form of cytochrome c oxidase: effects of detergent, the intact membrane, and radiation inactivation

    SciTech Connect

    Thompson, D.A.; Suarez-Villafane, M.; Ferguson-Miller, S.

    1982-01-01

    Cytochrome oxidase is a multisubunit, intrinsic membrane protein with a complex function that includes oxidation of cytochrome c, reduction of oxygen and generation of a membrane potential. To clarify the relationship of its normal function to protein and membrane structure, we have examined the kinetic behavior of rat liver cytochrome oxidase in the intact inner mitochondrial membrane and in detergent solubilized states. Dissolution of rat liver mitochondrial membranes alters the kinetic parameters of the oxidase in a manner dependent in part on the dispersing agent, and characterized by a large increase in maximal activity which is not attributable to exposure of more oxidase or diminished affinity for cytochrome c. The most profound effect of solubilization of the membrane is seen on the low affinity reaction of cytochrome c, suggesting that the electron transfer pathway from this site to oxygen is sensitive to alterations in hydrophobic interactions within the oxidase. Purified rat liver and beef heart oxidase exists predominantly in a monodisperse, 300 kilodalton form in laurylmaltoside (Rosevear et al., 1980). However, a smaller, 130 kd species that exhibits high turnover rates equal to the 300 kd form is detected in some beef heart preparations, implying that the dimer may not be essential for high activity. Radiation inactivation studies on purified oxidase reveal a molecular weight for the functional unit of approx.70 kd. It is concluded that less than a complete set of subunits may be sufficient for both normal binding of cytochrome c and rapid electron transfer to oxygen.

  12. Volatile sulphur compounds-forming abilities of lactic acid bacteria: C-S lyase activities.

    PubMed

    Bustos, Irene; Martínez-Bartolomé, Miguel A; Achemchem, Fouad; Peláez, Carmen; Requena, Teresa; Martínez-Cuesta, M Carmen

    2011-08-01

    Volatile sulphur compounds (VSCs) are of prime importance in the overall aroma of cheese and make a significant contribution to their typical flavours. Thus, the control of VSCs formation offers considerable potential for industrial applications. Here, lactic acid bacteria (LAB) from different ecological origins were screened for their abilities to produce VSCs from L-methionine. From the data presented, VSC-forming abilities were shown to be strain-specific and were correlated with the C-S lyase enzymatic activities determined using different approaches. High VSCs formation were detected for those strains that were also shown to possess high thiol-producing abilities (determined either by agar plate or spectrophotometry assays). Moreover, differences in C-S lyase activities were shown to correspond with the enzymatic potential of the strains as determined by in situ gel visualization. Therefore, the assessment of the C-S lyase enzymatic potential, by means of either of these techniques, could be used as a valuable approach for the selection of LAB strains with high VSC-producing abilities thus, representing an effective way to enhance cheese sulphur aroma compounds synthesis. In this regard, this study highlights the flavour forming potential of the Streptococcus thermophilus STY-31, that therefore could be used as a starter culture in cheese manufacture. Furthermore, although C-S lyases are involved in both biosynthetic and catabolic pathways, an association between methionine and cysteine auxotrophy of the selected strains and their VSCs-producing abilities could not be found.

  13. Piezo proteins are pore-forming subunits of mechanically activated channels.

    PubMed

    Coste, Bertrand; Xiao, Bailong; Santos, Jose S; Syeda, Ruhma; Grandl, Jörg; Spencer, Kathryn S; Kim, Sung Eun; Schmidt, Manuela; Mathur, Jayanti; Dubin, Adrienne E; Montal, Mauricio; Patapoutian, Ardem

    2012-02-19

    Mechanotransduction has an important role in physiology. Biological processes including sensing touch and sound waves require as-yet-unidentified cation channels that detect pressure. Mouse Piezo1 (MmPiezo1) and MmPiezo2 (also called Fam38a and Fam38b, respectively) induce mechanically activated cationic currents in cells; however, it is unknown whether Piezo proteins are pore-forming ion channels or modulate ion channels. Here we show that Drosophila melanogaster Piezo (DmPiezo, also called CG8486) also induces mechanically activated currents in cells, but through channels with remarkably distinct pore properties including sensitivity to the pore blocker ruthenium red and single channel conductances. MmPiezo1 assembles as a ∼1.2-million-dalton homo-oligomer, with no evidence of other proteins in this complex. Purified MmPiezo1 reconstituted into asymmetric lipid bilayers and liposomes forms ruthenium-red-sensitive ion channels. These data demonstrate that Piezo proteins are an evolutionarily conserved ion channel family involved in mechanotransduction.

  14. Modeling of chlorine effect on floc forming and filamentous micro-organisms of activated sludges.

    PubMed

    Caravelli, Alejandro; Contreras, Edgardo M; Giannuzzi, Leda; Zaritzky, Noemi

    2003-05-01

    Chlorination is the most economical, non-specific method to control the excessive growth of filamentous micro-organisms causing bulking in activated sludge systems in the treatment of food industrial wastewaters; it was one of the first methods used to control filamentous bulking and is still widely employed. Considering that chlorination affects both floc-forming and filamentous micro-organisms and leaves undesirable disinfection by-products, it is necessary to define the adequate doses to control bulking, minimizing the effect on floc-forming bacteria. In the present work the effect of biomass concentration and type of micro-organism on chlorine decay kinetics was evaluated; the inactivation of either a filamentous (Sphaerotilus natans) or a floc-forming (Acinetobacter anitratus) micro-organism due to chlorination was also analyzed. For chlorine decay assays, the samples were treated in a batch system with sodium hypochlorite ranging between 9.8 and 56.6 mg Cl(2) (gVSS)(-1). Respirometric assays were used to evaluate the effect of chlorine on micro-organisms respiratory activity; in these cases, sodium hypochlorite doses ranged between 2.5 and 18 mgCl(2) (gVSS)(-1).A model that allowed to predict simultaneously chlorine consumption and respiratory activity decay for both micro-organisms as a function of time was proposed. The model includes three coupled differential equations corresponding to respiratory inhibition, readily organic matter oxidation by chlorine and chlorine decay. The rate of chlorine decay depended on both, type and concentration of the micro-organisms in the system. Chlorine consumption rate due to S. natans was 2-4 times faster than A. anitratus. Using the proposed model initial critical chlorine doses (the lowest initial dose that leads to a total inhibition of the respiratory activity) were calculated for both micro-organisms and values of 11.9 mgCl(2) (gVSS)(-1) for S. natans and 4.5 mgCl(2) (gVSS)(-1) for A. anitratus were obtained. These

  15. Biofilm-forming activity of bacteria isolated from toilet bowl biofilms and the bactericidal activity of disinfectants against the isolates.

    PubMed

    Mori, Miho; Gomi, Mitsuhiro; Matsumune, Norihiko; Niizeki, Kazuma; Sakagami, Yoshikazu

    2013-01-01

    To evaluate the sanitary conditions of toilets, the bacterial counts of the toilet bowl biofilms in 5 Kansai area and 11 Kansai and Kanto area homes in Japan were measured in winter and summer seasons, respectively. Isolates (128 strains) were identified by analyzing 16S ribosomal RNA sequences. The number of colonies and bacterial species from biofilms sampled in winter tended to be higher and lower, respectively, than those in summer. Moreover, the composition of bacterial communities in summer and winter samples differed considerably. In summer samples, biofilms in Kansai and Kanto areas were dominated by Blastomonas sp. and Mycobacterium sp., respectively. Methylobacterium sp. was detected in all toilet bowl biofilms except for one sample. Methylobacterium sp. constituted the major presence in biofilms along with Brevundimonas sp., Sphingomonas sp., and/or Pseudomonas sp. The composition ratio of the sum of their genera was 88.0 from 42.9% of the total bacterial flora. The biofilm formation abilities of 128 isolates were investigated, and results suggested that Methylobacterium sp. and Sphingomonas sp. were involved in biofilm formation in toilet bowls. The biofilm formation of a mixed bacteria system that included bacteria with the highest biofilm-forming ability in a winter sample was greater than mixture without such bacteria. This result suggests that isolates possessing a high biofilm-forming activity are involved in the biofilm formation in the actual toilet bowl. A bactericidal test against 25 strains indicated that the bactericidal activities of didecyldimethylammonium chloride (DDAC) tended to be higher than those of polyhexamethylene biguanide (PHMB) and N-benzyl-N,N-dimethyldodecylammonium chloride (ADBAC). In particular, DDAC showed high bactericidal activity against approximately 90% of tested strains under the 5 h treatment.

  16. Extensive Post-translational Modification of Active and Inactivated Forms of Endogenous p53*

    PubMed Central

    DeHart, Caroline J.; Chahal, Jasdave S.; Flint, S. J.; Perlman, David H.

    2014-01-01

    The p53 tumor suppressor protein accumulates to very high concentrations in normal human fibroblasts infected by adenovirus type 5 mutants that cannot direct assembly of the viral E1B 55-kDa protein-containing E3 ubiquitin ligase that targets p53 for degradation. Despite high concentrations of nuclear p53, the p53 transcriptional program is not induced in these infected cells. We exploited this system to examine select post-translational modifications (PTMs) present on a transcriptionally inert population of endogenous human p53, as well as on p53 activated in response to etoposide treatment of normal human fibroblasts. These forms of p53 were purified from whole cell lysates by means of immunoaffinity chromatography and SDS-PAGE, and peptides derived from them were subjected to nano-ultra-high-performance LC-MS and MS/MS analyses on a high-resolution accurate-mass MS platform (data available via ProteomeXchange, PXD000464). We identified an unexpectedly large number of PTMs, comprising phosphorylation of Ser and Thr residues, methylation of Arg residues, and acetylation, ubiquitinylation, and methylation of Lys residues—for example, some 150 previously undescribed modifications of p53 isolated from infected cells. These modifications were distributed across all functional domains of both forms of the endogenous human p53 protein, as well as those of an orthologous population of p53 isolated from COS-1 cells. Despite the differences in activity, including greater in vitro sequence-specific DNA binding activity exhibited by p53 isolated from etoposide-treated cells, few differences were observed in the location, nature, or relative frequencies of PTMs on the two populations of human p53. Indeed, the wealth of PTMs that we have identified is consistent with a far greater degree of complex, combinatorial regulation of p53 by PTM than previously anticipated. PMID:24056736

  17. Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.

    PubMed

    Liu, Xing; Zhang, Zhibin; Ruan, Jianbin; Pan, Youdong; Magupalli, Venkat Giri; Wu, Hao; Lieberman, Judy

    2016-07-01

    Inflammatory caspases (caspases 1, 4, 5 and 11) are activated in response to microbial infection and danger signals. When activated, they cleave mouse and human gasdermin D (GSDMD) after Asp276 and Asp275, respectively, to generate an N-terminal cleavage product (GSDMD-NT) that triggers inflammatory death (pyroptosis) and release of inflammatory cytokines such as interleukin-1β. Cleavage removes the C-terminal fragment (GSDMD-CT), which is thought to fold back on GSDMD-NT to inhibit its activation. However, how GSDMD-NT causes cell death is unknown. Here we show that GSDMD-NT oligomerizes in membranes to form pores that are visible by electron microscopy. GSDMD-NT binds to phosphatidylinositol phosphates and phosphatidylserine (restricted to the cell membrane inner leaflet) and cardiolipin (present in the inner and outer leaflets of bacterial membranes). Mutation of four evolutionarily conserved basic residues blocks GSDMD-NT oligomerization, membrane binding, pore formation and pyroptosis. Because of its lipid-binding preferences, GSDMD-NT kills from within the cell, but does not harm neighbouring mammalian cells when it is released during pyroptosis. GSDMD-NT also kills cell-free bacteria in vitro and may have a direct bactericidal effect within the cytosol of host cells, but the importance of direct bacterial killing in controlling in vivo infection remains to be determined. PMID:27383986

  18. Auditory selective attention to speech modulates activity in the visual word form area.

    PubMed

    Yoncheva, Yuliya N; Zevin, Jason D; Maurer, Urs; McCandliss, Bruce D

    2010-03-01

    Selective attention to speech versus nonspeech signals in complex auditory input could produce top-down modulation of cortical regions previously linked to perception of spoken, and even visual, words. To isolate such top-down attentional effects, we contrasted 2 equally challenging active listening tasks, performed on the same complex auditory stimuli (words overlaid with a series of 3 tones). Instructions required selectively attending to either the speech signals (in service of rhyme judgment) or the melodic signals (tone-triplet matching). Selective attention to speech, relative to attention to melody, was associated with blood oxygenation level-dependent (BOLD) increases during functional magnetic resonance imaging (fMRI) in left inferior frontal gyrus, temporal regions, and the visual word form area (VWFA). Further investigation of the activity in visual regions revealed overall deactivation relative to baseline rest for both attention conditions. Topographic analysis demonstrated that while attending to melody drove deactivation equivalently across all fusiform regions of interest examined, attending to speech produced a regionally specific modulation: deactivation of all fusiform regions, except the VWFA. Results indicate that selective attention to speech can topographically tune extrastriate cortex, leading to increased activity in VWFA relative to surrounding regions, in line with the well-established connectivity between areas related to spoken and visual word perception in skilled readers.

  19. In Vitro Antimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm Forming Staphylococcus epidermidis Strains

    PubMed Central

    Wojtyczka, Robert D.; Kępa, Małgorzata; Idzik, Danuta; Kabała-Dzik, Agata; Wąsik, Tomasz J.

    2013-01-01

    The aim of the presented study was to examine the antimicrobial activity of ethanol extract of Polish propolis (EEPP) against biofilm-forming CoNS strains in vitro. Our results revealed that EEPP displayed varying degrees of activity against CoNS with MIC values ranging from 1.56 to 0.78 mg/mL. The average MIC was 1.13 ± 0.39 mg/mL while calculated MIC50 and MIC90 values were 0.78 mg/mL and 1.56 mg/mL, respectively. The biofilm formation ability by all tested S. epidermidis strains was inhibited at EEPP concentrations ranging from 0.39 to 1.56 mg/mL. The degree of reduction of AlamarBlue was directly associated with the proliferation of S. epidermidis strains. The increased proliferation of S. epidermidis strains was observed after 12 and 24 hours of incubation in the presence of EEPP concentrations ranging from 0.025 to 0.39 mg/mL. These results suggest that antimicrobial activities of EEPP against S. epidermidis expressed as the reduction of bacterial growth, reduction of biofilm formation ability, and the intensity of proliferation were significantly affected by incubation time and EEPP concentration used as well as the interactions between these factors. PMID:23662143

  20. FUNCTION FOLLOWS FORM: ACTIVATION OF SHAPE & FUNCTION FEATURES DURING OBJECT IDENTIFICATION

    PubMed Central

    Yee, Eiling; Huffstetler, Stacy; Thompson-Schill, Sharon L.

    2011-01-01

    Most theories of semantic memory characterize knowledge of a given object as comprising a set of semantic features. But how does conceptual activation of these features proceed during object identification? We present the results of a pair of experiments that demonstrate that object recognition is a dynamically unfolding process in which function follows form. We used eye movements to explore whether activating one object’s concept leads to the activation of others that share perceptual (shape) or abstract (function) features. Participants viewed four-picture displays and clicked on the picture corresponding to a heard word. In critical trials, the conceptual representation of one of the objects in the display was similar in shape or function (i.e., its purpose) to the heard word. Importantly, this similarity was not apparent in the visual depictions (e.g., for the target “frisbee,” the shape-related object was a triangular slice of pizza – a shape that a frisbee cannot take); preferential fixations on the related object were therefore attributable to overlap of the conceptual representations on the relevant features. We observed relatedness effects for both shape and function, but shape effects occurred earlier than function effects. We discuss the implications of these findings for current accounts of the representation of semantic memory. PMID:21417543

  1. Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.

    PubMed

    Liu, Xing; Zhang, Zhibin; Ruan, Jianbin; Pan, Youdong; Magupalli, Venkat Giri; Wu, Hao; Lieberman, Judy

    2016-07-06

    Inflammatory caspases (caspases 1, 4, 5 and 11) are activated in response to microbial infection and danger signals. When activated, they cleave mouse and human gasdermin D (GSDMD) after Asp276 and Asp275, respectively, to generate an N-terminal cleavage product (GSDMD-NT) that triggers inflammatory death (pyroptosis) and release of inflammatory cytokines such as interleukin-1β. Cleavage removes the C-terminal fragment (GSDMD-CT), which is thought to fold back on GSDMD-NT to inhibit its activation. However, how GSDMD-NT causes cell death is unknown. Here we show that GSDMD-NT oligomerizes in membranes to form pores that are visible by electron microscopy. GSDMD-NT binds to phosphatidylinositol phosphates and phosphatidylserine (restricted to the cell membrane inner leaflet) and cardiolipin (present in the inner and outer leaflets of bacterial membranes). Mutation of four evolutionarily conserved basic residues blocks GSDMD-NT oligomerization, membrane binding, pore formation and pyroptosis. Because of its lipid-binding preferences, GSDMD-NT kills from within the cell, but does not harm neighbouring mammalian cells when it is released during pyroptosis. GSDMD-NT also kills cell-free bacteria in vitro and may have a direct bactericidal effect within the cytosol of host cells, but the importance of direct bacterial killing in controlling in vivo infection remains to be determined.

  2. 77 FR 65703 - Agency Information Collection Activities: Application for Family Unity Benefits, Form I-817...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... for Family Unity Benefits, Form I-817, Revision of a Currently Approved Collection ACTION: 60-Day...) Title of the Form/Collection: Application for Family Unity Benefits. (3) Agency form number, if any,...

  3. ESR dating of barite in sulphide deposits formed by the sea-floor hydrothermal activities.

    PubMed

    Toyoda, Shin; Fujiwara, Taisei; Uchida, Ai; Ishibashi, Jun-ichiro; Nakai, Shun'ichi; Takamasa, Asako

    2014-06-01

    Barite is a mineral newly found to be practically useful for electron spin resonance (ESR) dating of sulphide deposits formed by the sea-floor hydrothermal activities. The recent studies for the properties of the ESR dating signal in barite are summarised in the present paper as well as the formulas for corrections for accurate dose-rate estimation are developed including the dose-rate conversion factors, shape correction for gamma-ray dose and decay of (226)Ra. Although development of the techniques for ESR dating of barite has been completed, further comparative studies with other dating techniques such as U-Th and (226)Ra-(210)Pb dating are necessary for the technique to be widely used.

  4. Magnetic field enhancement of antibiotic activity in biofilm forming Pseudomonas aeruginosa.

    PubMed

    Benson, D E; Grissom, C B; Burns, G L; Mohammad, S F

    1994-01-01

    Device related infection initiated by biofilm bacteria are often difficult to resolve with antimicrobial therapy. Study results indicate that application of static magnetic fields may enhance the activity of gentamicin against biofilm forming Pseudomonas aeruginosa adherent to a polymer substrate. Results indicate a maximal reduction of 86.5 +/- 7.2% (n = 6) in the number of adherent viable bacteria compared with a control for samples exposed to a 5 gauss (G) magnetic field and gentamicin. The effect appears to be limited to magnetic fields between 5 and 20 G. Experiments using glass, Chronoflex (Polymedica, Golden, CO), Biomer (Ethicon, Somerville, NJ), and polystyrene substrate showed that the effect was independent of substrate surface. Autoradiograms from In111 uptake experiments showed that bacteria colonizing the substrate surface were significantly reduced in samples subjected to a magnetic field and gentamicin. PMID:8555541

  5. Identification of Triclosan-O-Sulfate and other transformation products of Triclosan formed by activated sludge.

    PubMed

    Chen, Xijuan; Casas, Mònica Escolà; Nielsen, Jeppe Lund; Wimmer, Reinhard; Bester, Kai

    2015-02-01

    Aerobic degradation experiments of Triclosan were performed in activated sludge to identify possible transformation products for this compound. During 7 days, the formation of biotransformation products such as 2,4-Dichlorophenol, 4-Chlorocatechol, 5-Hydroxy-Triclosan and other Monohydroxy-Triclosan derivatives as well as Dihydroxy-Triclosan-derivatives were observed. The structure of 5-Hydroxy-Triclosan was elucidated by NMR data for the first time in sludge degradation experiments. Additionally the production of a hitherto unknown transformation product in sludge, i.e., Triclosan-O-Sulfate was detected. During the incubations, the concentrations of this transformation product changed from zero to 330 μg L(-1). Based on the analysis of the biodegradation products, three types of reactions were identified: 1) chemical scission of ether bond to form phenols and catechols, 2) addition of OH moieties to the aromatic ring, and 3) adding of methyl or sulfate groups to the original hydroxyl group.

  6. The Role of Biofilms in the Sedimentology of Actively Forming Gypsum Deposits at Guerrero Negro, Mexico

    NASA Astrophysics Data System (ADS)

    Vogel, Marilyn B.; Des Marais, David J.; Turk, Kendra A.; Parenteau, Mary N.; Jahnke, Linda L.; Kubo, Michael D. Y.

    2009-11-01

    Actively forming gypsum deposits at the Guerrero Negro sabkha and saltern system provided habitats for stratified, pigmented microbial communities that exhibited significant morphological and phylogenetic diversity. These deposits ranged from meter-thick gypsum crusts forming in saltern seawater concentration ponds to columnar microbial mats with internally crystallized gypsum granules developing in natural anchialine pools. Gypsum-depositing environments were categorized as forming precipitation surfaces, biofilm-supported surfaces, and clastic surfaces. Each surface type was described in terms of depositional environment, microbial diversity, mineralogy, and sedimentary fabrics. Precipitation surfaces developed in high-salinity subaqueous environments where rates of precipitation outpaced the accumulation of clastic, organic, and/or biofilm layers. These surfaces hosted endolithic biofilms comprised predominantly of oxygenic and anoxygenic phototrophs, sulfate-reducing bacteria, and bacteria from the phylum Bacteroidetes. Biofilm-supported deposits developed in lower-salinity subaqueous environments where light and low water-column turbulence supported dense benthic microbial communities comprised mainly of oxygenic phototrophs. In these settings, gypsum granules precipitated in the extracellular polymeric substance (EPS) matrix as individual granules exhibiting distinctive morphologies. Clastic surfaces developed in sabkha mudflats that included gypsum, carbonate, and siliclastic particles with thin gypsum/biofilm components. Clastic surfaces were influenced by subsurface brine sheets and capillary evaporation and precipitated subsedimentary gypsum discs in deeper regions. Biofilms appeared to influence both chemical and physical sedimentary processes in the various subaqueous and subaerially exposed environments studied. Biofilm interaction with chemical sedimentary processes included dissolution and granularization of precipitation surfaces, formation of

  7. The role of biofilms in the sedimentology of actively forming gypsum deposits at Guerrero Negro, Mexico.

    PubMed

    Vogel, Marilyn B; Des Marais, David J; Turk, Kendra A; Parenteau, Mary N; Jahnke, Linda L; Kubo, Michael D Y

    2009-11-01

    Actively forming gypsum deposits at the Guerrero Negro sabkha and saltern system provided habitats for stratified, pigmented microbial communities that exhibited significant morphological and phylogenetic diversity. These deposits ranged from meter-thick gypsum crusts forming in saltern seawater concentration ponds to columnar microbial mats with internally crystallized gypsum granules developing in natural anchialine pools. Gypsum-depositing environments were categorized as forming precipitation surfaces, biofilm-supported surfaces, and clastic surfaces. Each surface type was described in terms of depositional environment, microbial diversity, mineralogy, and sedimentary fabrics. Precipitation surfaces developed in high-salinity subaqueous environments where rates of precipitation outpaced the accumulation of clastic, organic, and/or biofilm layers. These surfaces hosted endolithic biofilms comprised predominantly of oxygenic and anoxygenic phototrophs, sulfate-reducing bacteria, and bacteria from the phylum Bacteroidetes. Biofilm-supported deposits developed in lower-salinity subaqueous environments where light and low water-column turbulence supported dense benthic microbial communities comprised mainly of oxygenic phototrophs. In these settings, gypsum granules precipitated in the extracellular polymeric substance (EPS) matrix as individual granules exhibiting distinctive morphologies. Clastic surfaces developed in sabkha mudflats that included gypsum, carbonate, and siliclastic particles with thin gypsum/biofilm components. Clastic surfaces were influenced by subsurface brine sheets and capillary evaporation and precipitated subsedimentary gypsum discs in deeper regions. Biofilms appeared to influence both chemical and physical sedimentary processes in the various subaqueous and subaerially exposed environments studied. Biofilm interaction with chemical sedimentary processes included dissolution and granularization of precipitation surfaces, formation of

  8. The role of biofilms in the sedimentology of actively forming gypsum deposits at Guerrero Negro, Mexico.

    PubMed

    Vogel, Marilyn B; Des Marais, David J; Turk, Kendra A; Parenteau, Mary N; Jahnke, Linda L; Kubo, Michael D Y

    2009-11-01

    Actively forming gypsum deposits at the Guerrero Negro sabkha and saltern system provided habitats for stratified, pigmented microbial communities that exhibited significant morphological and phylogenetic diversity. These deposits ranged from meter-thick gypsum crusts forming in saltern seawater concentration ponds to columnar microbial mats with internally crystallized gypsum granules developing in natural anchialine pools. Gypsum-depositing environments were categorized as forming precipitation surfaces, biofilm-supported surfaces, and clastic surfaces. Each surface type was described in terms of depositional environment, microbial diversity, mineralogy, and sedimentary fabrics. Precipitation surfaces developed in high-salinity subaqueous environments where rates of precipitation outpaced the accumulation of clastic, organic, and/or biofilm layers. These surfaces hosted endolithic biofilms comprised predominantly of oxygenic and anoxygenic phototrophs, sulfate-reducing bacteria, and bacteria from the phylum Bacteroidetes. Biofilm-supported deposits developed in lower-salinity subaqueous environments where light and low water-column turbulence supported dense benthic microbial communities comprised mainly of oxygenic phototrophs. In these settings, gypsum granules precipitated in the extracellular polymeric substance (EPS) matrix as individual granules exhibiting distinctive morphologies. Clastic surfaces developed in sabkha mudflats that included gypsum, carbonate, and siliclastic particles with thin gypsum/biofilm components. Clastic surfaces were influenced by subsurface brine sheets and capillary evaporation and precipitated subsedimentary gypsum discs in deeper regions. Biofilms appeared to influence both chemical and physical sedimentary processes in the various subaqueous and subaerially exposed environments studied. Biofilm interaction with chemical sedimentary processes included dissolution and granularization of precipitation surfaces, formation of

  9. Cooperation of Doxycycline with Phytochemicals and Micronutrients Against Active and Persistent Forms of Borrelia sp

    PubMed Central

    Goc, Anna; Niedzwiecki, Alexandra; Rath, Matthias

    2016-01-01

    Phytochemicals and micronutrients represent a growing theme in antimicrobial defense; however, little is known about their anti-borreliae effects of reciprocal cooperation with antibiotics. A better understanding of this aspect could advance our knowledge and help improve the efficacy of current approaches towards Borrelia sp. In this study, phytochemicals and micronutrients such as baicalein, luteolin, 10-HAD, iodine, rosmarinic acid, and monolaurin, as well as, vitamins D3 and C were tested in a combinations with doxycycline for their in vitro effectiveness against vegetative (spirochetes) and latent (rounded bodies, biofilm) forms of Borrelia burgdorferi and Borrelia garinii. Anti-borreliae effects were evaluated according to checkerboard assays and supported by statistical analysis. The results showed that combination of doxycycline with flavones such as baicalein and luteolin exhibited additive effects against all morphological forms of studied Borrelia sp. Doxycycline combined with iodine demonstrated additive effects against spirochetes and biofilm, whereas with fatty acids such as monolaurin and 10-HAD it produced FICIs of indifference. Additive anti-spirochetal effects were also observed when doxycycline was used with rosmarinic acid and both vitamins D3 and C. Antagonism was not observed in any of the cases. This data revealed the intrinsic anti-borreliae activity of doxycycline with tested phytochemicals and micronutrients indicating that their addition may enhance efficacy of this antibiotic in combating Borrelia sp. Especially the addition of flavones balcalein and luteolin to a doxycycline regimen could be explored further in defining more effective treatments against these bacteria. PMID:27570483

  10. Cooperation of Doxycycline with Phytochemicals and Micronutrients Against Active and Persistent Forms of Borrelia sp.

    PubMed

    Goc, Anna; Niedzwiecki, Alexandra; Rath, Matthias

    2016-01-01

    Phytochemicals and micronutrients represent a growing theme in antimicrobial defense; however, little is known about their anti-borreliae effects of reciprocal cooperation with antibiotics. A better understanding of this aspect could advance our knowledge and help improve the efficacy of current approaches towards Borrelia sp. In this study, phytochemicals and micronutrients such as baicalein, luteolin, 10-HAD, iodine, rosmarinic acid, and monolaurin, as well as, vitamins D3 and C were tested in a combinations with doxycycline for their in vitro effectiveness against vegetative (spirochetes) and latent (rounded bodies, biofilm) forms of Borrelia burgdorferi and Borrelia garinii. Anti-borreliae effects were evaluated according to checkerboard assays and supported by statistical analysis. The results showed that combination of doxycycline with flavones such as baicalein and luteolin exhibited additive effects against all morphological forms of studied Borrelia sp. Doxycycline combined with iodine demonstrated additive effects against spirochetes and biofilm, whereas with fatty acids such as monolaurin and 10-HAD it produced FICIs of indifference. Additive anti-spirochetal effects were also observed when doxycycline was used with rosmarinic acid and both vitamins D3 and C. Antagonism was not observed in any of the cases. This data revealed the intrinsic anti-borreliae activity of doxycycline with tested phytochemicals and micronutrients indicating that their addition may enhance efficacy of this antibiotic in combating Borrelia sp. Especially the addition of flavones balcalein and luteolin to a doxycycline regimen could be explored further in defining more effective treatments against these bacteria. PMID:27570483

  11. Electrically active centers formed in silicon during the high-temperature diffusion of boron and aluminum

    SciTech Connect

    Sobolev, N. A.; Loshachenko, A. S.; Poloskin, D. S.; Shek, E. I.

    2013-02-15

    The parameters of electrically active centers formed during the high-temperature diffusion of boron and aluminum into silicon in various media are studied by the Hall method and capacitance spectroscopy. It is found that the variation in the resistivity of the n base of the structures with p-n junctions fabricated in the study is controlled by the formation of three donor levels Q1, E4, and Q3 with the energies E{sub c} - 0.31, E{sub c} - 0.27, and E{sub c} - 0.16 eV. Diffusion in a chlorine-containing atmosphere introduces only a single level E4, but its concentration is 2.5 times lower, compared with diffusion in air. The values of the ionization energy of the Q3 level, measured under equilibrium (Hall effect) and nonequilibrium (capacitance spectroscopy) conditions, almost coincide. The deepest level E1 with an energy of E{sub c} - 0.54 eV, formed upon diffusion in both media, has no effect on the resistivity in the n base of the structures.

  12. Activation of human natural killer cells by the soluble form of cellular prion protein

    SciTech Connect

    Seong, Yeon-Jae; Sung, Pil Soo; Jang, Young-Soon; Choi, Young Joon; Park, Bum-Chan; Park, Su-Hyung; Park, Young Woo; Shin, Eui-Cheol

    2015-08-21

    Cellular prion protein (PrP{sup C}) is widely expressed in various cell types, including cells of the immune system. However, the specific roles of PrP{sup C} in the immune system have not been clearly elucidated. In the present study, we investigated the effects of a soluble form of recombinant PrP{sup C} protein on human natural killer (NK) cells. Recombinant soluble PrP{sup C} protein was generated by fusion of human PrP{sup C} with the Fc portion of human IgG{sub 1} (PrP{sup C}-Fc). PrP{sup C}-Fc binds to the surface of human NK cells, particularly to CD56{sup dim} NK cells. PrP{sup C}-Fc induced the production of cytokines and chemokines and the degranulation of granzyme B from NK cells. In addition, PrP{sup C}-Fc facilitated the IL-15-induced proliferation of NK cells. PrP{sup C}-Fc induced phosphorylation of ERK-1/2 and JNK in NK cells, and inhibitors of the ERK or the JNK pathways abrogated PrP{sup C}-Fc-induced cytokine production in NK cells. In conclusion, the soluble form of recombinant PrP{sup C}-Fc protein activates human NK cells via the ERK and JNK signaling pathways. - Highlights: • Recombinant soluble PrP{sup C} (PrP{sup C}-Fc) was generated by fusion of human PrP{sup C} with IgG1 Fc portion. • PrP{sup C}-Fc protein induces the production of cytokines and degranulation from human NK cells. • PrP{sup C}-Fc protein enhances the IL-15-induced proliferation of human NK cells. • PrP{sup C}-Fc protein activates human NK cells via the ERK and JNK signaling pathways.

  13. CTEPP DATA COLLECTION FORM 09 (PERIODS 1-4 AND FOOD, FRUIT & VEG): CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data collection form is divided into two parts: Child Activity Diary and Food Survey. The Child Activity Diary collects information on the child's activities at home over the 48-hr monitoring period. The diary is divided into four time periods over the 48-hr monitoring inter...

  14. CTEPP DATA COLLECTION FORM 08 (PERIODS 1-5 AND FOOD, FRUIT & VEG): CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data collection form is divided into two parts: Child Activity Diary and Food Survey. The Child Activity Diary collects information on the child's activities at home over the 48-hr monitoring period. The diary is divided into five time periods over the 48-hr monitoring inter...

  15. Alarin but not its alternative-splicing form, GALP (Galanin-like peptide) has antimicrobial activity

    SciTech Connect

    Wada, Akihiro; Wong, Pooi-Fong; Hojo, Hironobu; Hasegawa, Makoto; Ichinose, Akitoyo; Llanes, Rafael; Kubo, Yoshinao; Senba, Masachika; Ichinose, Yoshio

    2013-05-03

    Highlights: • Alarin inhibits the growth of E. coli but not S. aureus. • Alarin’s potency is comparable to LL-37 in inhibiting the growth of E. coli. • Alarin can cause bacterial membrane blebbing. • Alalin does not induce hemolysis on erythrocytes. -- Abstract: Alarin is an alternative-splicing form of GALP (galanin-like peptide). It shares only 5 conserved amino acids at the N-terminal region with GALP which is involved in a diverse range of normal brain functions. This study seeks to investigate whether alarin has additional functions due to its differences from GALP. Here, we have shown using a radial diffusion assay that alarin but not GALP inhibited the growth of Escherichia coli (strain ML-35). The conserved N-terminal region, however, remained essential for the antimicrobial activity of alarin as truncated peptides showed reduced killing effect. Moreover, alarin inhibited the growth of E. coli in a similar potency as human cathelicidin LL-37, a well-studied antimicrobial peptide. Electron microscopy further showed that alarin induced bacterial membrane blebbing but unlike LL-37, it did not cause hemolysis of erythrocytes. In addition, alarin is only active against the gram-negative bacteria, E. coli but not the gram-positive bacteria, Staphylococcus aureus. Thus, these data suggest that alarin has potentials as an antimicrobial and should be considered for the development in human therapeutics.

  16. Optogenetic activation of presynaptic inputs in lateral amygdala forms associative fear memory.

    PubMed

    Kwon, Jeong-Tae; Nakajima, Ryuichi; Kim, Hyung-Su; Jeong, Yire; Augustine, George J; Han, Jin-Hee

    2014-11-01

    In Pavlovian fear conditioning, the lateral amygdala (LA) has been highlighted as a key brain site for association between sensory cues and aversive stimuli. However, learning-related changes are also found in upstream sensory regions such as thalamus and cortex. To isolate the essential neural circuit components for fear memory association, we tested whether direct activation of presynaptic sensory inputs in LA, without the participation of upstream activity, is sufficient to form fear memory in mice. Photostimulation of axonal projections from the two main auditory brain regions, the medial geniculate nucleus of the thalamus and the secondary auditory cortex, was paired with aversive footshock. Twenty-four hours later the same photostimulation induced robust conditioned freezing and this fear memory formation was disrupted when glutamatergic synaptic transmission was locally blocked in the LA. Therefore, our results prove for the first time that synapses between sensory input areas and the LA, previously implicated as a crucial brain site for fear memory formation, actually are sufficient to serve as a conditioned stimulus. Our results strongly support the idea that the LA may be sufficient to encode and store associations between neutral cue and aversive stimuli during natural fear conditioning as a critical part of a broad fear memory engram.

  17. Antibody nanoparticle dispersions formed with mixtures of crowding molecules retain activity and in vivo bioavailability

    PubMed Central

    Miller, Maria A.; Khan, Tarik A.; Kaczorowski, Kevin J.; Wilson, Brian K.; Dinin, Aileen K.; Borwankar, Ameya U.; Rodrigues, Miguel A.; Truskett, Thomas M.; Johnston, Keith P.; Maynard, Jennifer A.

    2013-01-01

    Monoclonal antibodies continue to command a large market for treatment of a variety of diseases. In many cases, the doses required for therapeutic efficacy are large, limiting options for antibody delivery and administration. We report a novel formulation strategy based on dispersions of antibody nanoclusters that allows for subcutaneous injection of highly concentrated antibody (~190 mg/ml). A solution of monoclonal antibody 1B7 was rapidly frozen and lyophilized using a novel spiral-wound in situ freezing technology (SWIFT) to generate amorphous particles. Upon gentle stirring, a translucent dispersion of ~430 nm protein clusters low apparent viscosity (~24 cp) formed rapidly in buffer containing the pharmaceutically acceptable crowding agents, trehalose, polyethylene glycol and n-methyl-2-pyrrolidone. Upon in vitro dilution of the dispersion, the nanoclusters rapidly reverted to monomeric protein with full activity, as monitored by dynamic light scattering and antigen binding. When administered to mice as an intravenous solution, subcutaneous solution or subcutaneous dispersion at similar (4.6-7.3 mg/kg) or ultra-high dosages (51.6 mg/kg), the distribution and elimination kinetics were within error and the protein retained full activity. Overall, this method of generating high-concentration, low-viscosity dispersions of antibody nanoclusters could lead to improved administration and patient compliance, providing new opportunities for the biotechnology industry. PMID:22777686

  18. Antibody nanoparticle dispersions formed with mixtures of crowding molecules retain activity and in vivo bioavailability.

    PubMed

    Miller, Maria A; Khan, Tarik A; Kaczorowski, Kevin J; Wilson, Brian K; Dinin, Aileen K; Borwankar, Ameya U; Rodrigues, Miguel A; Truskett, Thomas M; Johnston, Keith P; Maynard, Jennifer A

    2012-10-01

    Monoclonal antibodies continue to command a large market for treatment of a variety of diseases. In many cases, the doses required for therapeutic efficacy are large, limiting options for antibody delivery and administration. We report a novel formulation strategy based on dispersions of antibody nanoclusters that allows for subcutaneous injection of highly concentrated antibody (≈ 190 mg/mL). A solution of monoclonal antibody 1B7 was rapidly frozen and lyophilized using a novel spiral-wound in-situ freezing technology to generate amorphous particles. Upon gentle stirring, a translucent dispersion of approximately 430 nm protein clusters with low apparent viscosity (≈ 24 cp) formed rapidly in buffer containing the pharmaceutically acceptable crowding agents such as trehalose, polyethylene glycol, and n-methyl-2-pyrrolidone. Upon in vitro dilution of the dispersion, the nanoclusters rapidly reverted to monomeric protein with full activity, as monitored by dynamic light scattering and antigen binding. When administered to mice as an intravenous solution, subcutaneous solution, or subcutaneous dispersion at similar (4.6-7.3 mg/kg) or ultra-high dosages (51.6 mg/kg), the distribution and elimination kinetics were within error and the protein retained full activity. Overall, this method of generating high-concentration, low-viscosity dispersions of antibody nanoclusters could lead to improved administration and patient compliance, providing new opportunities for the biotechnology industry.

  19. WIDESPREAD AND HIDDEN ACTIVE GALACTIC NUCLEI IN STAR-FORMING GALAXIES AT REDSHIFT >0.3

    SciTech Connect

    Juneau, Stephanie; Bournaud, Frederic; Daddi, Emanuele; Elbaz, David; Alexander, David M.; Mullaney, James R.; Magnelli, Benjamin; Hwang, Ho Seong; Willner, S. P.; Coil, Alison L.; Rosario, David J.; Trump, Jonathan R.; Faber, S. M.; Kocevski, Dale D.; Cooper, Michael C.; Frayer, David T.; and others

    2013-02-20

    We characterize the incidence of active galactic nuclei (AGNs) in 0.3 < z < 1 star-forming galaxies by applying multi-wavelength AGN diagnostics (X-ray, optical, mid-infrared, radio) to a sample of galaxies selected at 70 {mu}m from the Far-Infrared Deep Extragalactic Legacy survey (FIDEL). Given the depth of FIDEL, we detect 'normal' galaxies on the specific star formation rate (sSFR) sequence as well as starbursting systems with elevated sSFR. We find an overall high occurrence of AGN of 37% {+-} 3%, more than twice as high as in previous studies of galaxies with comparable infrared luminosities and redshifts but in good agreement with the AGN fraction of nearby (0.05 < z < 0.1) galaxies of similar infrared luminosities. The more complete census of AGNs comes from using the recently developed Mass-Excitation (MEx) diagnostic diagram. This optical diagnostic is also sensitive to X-ray weak AGNs and X-ray absorbed AGNs, and reveals that absorbed active nuclei reside almost exclusively in infrared-luminous hosts. The fraction of galaxies hosting an AGN appears to be independent of sSFR and remains elevated both on the sSFR sequence and above. In contrast, the fraction of AGNs that are X-ray absorbed increases substantially with increasing sSFR, possibly due to an increased gas fraction and/or gas density in the host galaxies.

  20. Characterization of a soluble, catalytically active form of Escherichia coli leader peptidase: requirement of detergent or phospholipid for optimal activity.

    PubMed

    Tschantz, W R; Paetzel, M; Cao, G; Suciu, D; Inouye, M; Dalbey, R E

    1995-03-28

    Leader peptidase is a novel serine protease in Escherichia coli, which functions to cleave leader sequences from exported proteins. Its catalytic domain extends into the periplasmic space and is anchored to the membrane by two transmembrane segments located at the N-terminal end of the protein. At present, there is no information on the structure of the catalytic domain. Here, we report on the properties of a soluble form of leader peptidase (delta 2-75), and we compare its properties to those of the wild-type enzyme. We find that the truncated leader peptidase has a kcat of 3.0 S-1 and a Km of 32 microM with a pro-OmpA nuclease A substrate. In contrast to the wild-type enzyme (pI of 6.8), delta 2-75 is water-soluble and has an acidic isoelectric point of 5.6. We also show with delta 2-75 that the replacement of serine 90 and lysine 145 with alanine residues results in a 500-fold reduction in activity, providing further evidence that leader peptidase employs a catalytic serine/lysine dyad. Finally, we find that the catalysis of delta 2-75 is accelerated by the presence of the detergent Triton X-100, regardless if the substrate is pro-OmpA nuclease A or a peptide substrate. Triton X-100 is required for optimal activity of delta 2-75 at a level far below the critical micelle concentration. Moreover, we find that E. coli phospholipids stimulate the activity of delta 2-75, suggesting that phospholipids may play an important physiological role in the catalytic mechanism of leader peptidase. PMID:7696258

  1. 77 FR 65706 - Agency Information Collection Activities: Immigrant Petition for Alien Worker, Form I-140...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... Petition for Alien Worker, Form I-140; Revision of a Currently Approved Collection ACTION: 60-Day Notice... Form/Collection: Immigrant Petition for Alien Worker. (3) Agency form number, if any, and the... information furnished on Form I-140 will be used by USCIS to classify aliens under sections 203(b)(1),...

  2. Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes

    PubMed Central

    Crofts, Naoko; Abe, Natsuko; Oitome, Naoko F.; Matsushima, Ryo; Hayashi, Mari; Tetlow, Ian J.; Emes, Michael J.; Nakamura, Yasunori; Fujita, Naoko

    2015-01-01

    Amylopectin is a highly branched, organized cluster of glucose polymers, and the major component of rice starch. Synthesis of amylopectin requires fine co-ordination between elongation of glucose polymers by soluble starch synthases (SSs), generation of branches by branching enzymes (BEs), and removal of misplaced branches by debranching enzymes (DBEs). Among the various isozymes having a role in amylopectin biosynthesis, limited numbers of SS and BE isozymes have been demonstrated to interact via protein–protein interactions in maize and wheat amyloplasts. This study investigated whether protein–protein interactions are also found in rice endosperm, as well as exploring differences between species. Gel permeation chromatography of developing rice endosperm extracts revealed that all 10 starch biosynthetic enzymes analysed were present at larger molecular weights than their respective monomeric sizes. SSIIa, SSIIIa, SSIVb, BEI, BEIIb, and PUL co-eluted at mass sizes >700kDa, and SSI, SSIIa, BEIIb, ISA1, PUL, and Pho1 co-eluted at 200–400kDa. Zymogram analyses showed that SSI, SSIIIa, BEI, BEIIa, BEIIb, ISA1, PUL, and Pho1 eluted in high molecular weight fractions were active. Comprehensive co-immunoprecipitation analyses revealed associations of SSs–BEs, and, among BE isozymes, BEIIa–Pho1, and pullulanase-type DBE–BEI interactions. Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes. These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes. Detailed analyses of these complexes will shed light on the mechanisms controlling the unique branch and cluster structure of amylopectin, and the physicochemical properties of starch. PMID:25979995

  3. Enhanced bone-forming activity of side population cells in the periodontal ligament.

    PubMed

    Ninomiya, Tadashi; Hiraga, Toru; Hosoya, Akihiro; Ohnuma, Kiyoshi; Ito, Yuzuru; Takahashi, Masafumi; Ito, Susumu; Asashima, Makoto; Nakamura, Hiroaki

    2014-04-01

    Regeneration of alveolar bone is critical for the successful treatment of periodontal diseases. The periodontal ligament (PDL) has been widely investigated as a source of cells for the regeneration of periodontal tissues. In the present study where we attempted to develop an effective strategy for alveolar bone regeneration, we examined the osteogenic potential of side population (SP) cells, a stem cell-containing population that has been shown to be highly abundant in several kinds of tissues, in PDL cells. Isolated SP cells from the rat PDL exhibited a superior ability to differentiate into osteoblastic cells compared with non-SP (NSP) and unsorted PDL cells in vitro. The mRNA expressions of osteoblast markers and bone morphogenetic protein (BMP) 2 were significantly upregulated in SP cells and were further increased by osteogenic induction. To examine the bone-forming activity of SP cells in vivo, PDL SP cells isolated from green fluorescent protein (GFP)-transgenic rats were transplanted with hydroxyapatite (HA) disks into wild-type animals. SP cells exhibited a high ability to induce the mineralized matrix compared with NSP and unsorted PDL cells. At 12 weeks after the implantation, some of the pores in the HA disks with SP cells were filled with mineralized matrices, which were positive for bone matrix proteins, such as osteopontin, bone sialoprotein, and osteocalcin. Furthermore, osteoblast- and osteocyte-like cells on and in the bone-like mineralized matrices were GFP positive, suggesting that the matrices were directly formed by the transplanted cells. These results suggest that PDL SP cells possess enhanced osteogenic potential and could be a potential source for cell-based regenerative therapy for alveolar bone.

  4. Purified TMEM16A is sufficient to form Ca2+-activated Cl− channels

    PubMed Central

    Terashima, Hiroyuki; Picollo, Alessandra; Accardi, Alessio

    2013-01-01

    Ca2+-activated Cl− channels (CaCCs) are key regulators of numerous physiological functions, ranging from electrolyte secretion in airway epithelia to cellular excitability in sensory neurons and muscle fibers. Recently, TMEM16A (ANO1) and -B were shown to be critical components of CaCCs. It is still unknown whether they are also sufficient to form functional CaCCs, or whether association with other subunits is required. Recent reports suggest that the Ca2+ sensitivity of TMEM16A is mediated by its association with calmodulin, suggesting that functional CaCCs are heteromultimers. To test whether TMEM16A is necessary and sufficient to form functional CaCCs, we expressed, purified, and reconstituted human TMEM16A. The purified protein mediates Ca2+-dependent Cl− transport with submicromolar sensitivity to Ca2+, consistent with what is seen in patch–clamp experiments. The channel is synergistically gated by Ca2+ and voltage, so that opening is promoted by depolarizing potentials. Mutating two conserved glutamates in the TM6-7 intracellular loop selectively abolishes the Ca2+ dependence of reconstituted TMEM16A, in a manner similar to what was reported for the heterologously expressed channel. Well-characterized CaCC blockers inhibit Cl− transport with Kis comparable to those measured for native and heterologously expressed CaCCs. Finally, direct physical interactions between calmodulin and TMEM16A could not be detected in copurification experiments or in functional assays. Our results demonstrate that purified TMEM16A is necessary and sufficient to recapitulate the biophysical and pharmacological properties of native and heterologously expressed CaCCs. Our results also show that association of TMEM16A with other proteins, such as calmodulin, is not required for function. PMID:24167264

  5. Light activated, In situ Forming Gel for Sustained Suprachoroidal Delivery of Bevacizumab

    PubMed Central

    Tyagi, Puneet; Barros, Matthew; Stansbury, Jeffrey W.; Kompella, Uday B.

    2014-01-01

    Purpose To develop a light activated polycaprolactone dimethacrylate and hydroxyethyl methacrylate based gel network that sustains the release of stable, active bevacizumab (an anti-VEGF antibody used to treat choroidal neovascularization) and to assess sustained ex vivo delivery in rabbit eyes and in vivo delivery in rat eyes following in situ gel formation in the suprachoroidal space. Methods Polycaprolactone dimethacrylate (PCM) was synthesized from polycaprolactone diol (PCD) and evaluated using NMR spectroscopy. PCM was used to cross-link hydroxyethyl methacrylate (HEMA) in the presence of 365 nm UV light and 2, 2-dimethoxy-2-phenylacetophenone (DMPA) as a photoinitiator. Bevacizumab was entrapped in the gel using 3 different cross-linking durations of 3, 7, and 10 minutes. In vitro release of bevacizumab in PBS pH 7.4 at 37°C during a 4 months study was quantified using a VEGF-binding based ELISA. Stability of released bevacizumab was monitored by size exclusion chromatography (SEC) and circular dichroism. Alexa Fluor® 488 dye conjugated bevacizumab mixed with polymers was injected suprachoroidally in rabbit eyes to study the effect of different cross-linking durations on the spread of the dye conjugated bevacizumab. In vivo delivery was assessed in Sprague Dawley (SD) rats by injecting Alexa Fluor® 488 dye conjugated bevacizumab mixed with polymers followed by cross-linking for 10 minutes. Spread in the rabbit eyes and in vivo delivery in rat eyes was monitored noninvasively using a fundus camera and Fluorotron Master™. Results Formation of PCM was confirmed by the disappearance of hydroxyl peak in NMR spectra. Cross-linking duration of 10 minutes resulted in a burst release of 21 % of bevacizumab. Other cross-linking durations had ≥ 62 % burst release. Bevacizumab release from 10 minute cross-linked gel was sustained for ∼ 4 months. Release samples contained ≥ 96.1 % of bevacizumab in the monomeric form as observed in SEC chromatograms. Circular

  6. Oscillations of solar activity - the main climate-forming factor in millennium scale.

    NASA Astrophysics Data System (ADS)

    Khorozov, S. V.; Budovy, V. I.; Medvedev, V. A.

    Here is advanced and substantiated the hypothesis of existence of the physical mechanism, by means of which alteration of solar activity leads to change of the "greenhouse effect". This hypothetical mechanism can be the following one. The increase of solar activity leads to considerable magnification of ultra-violet radiation in a solar spectrum and to increase of ion concentration in the upper stratums of an atmosphere (including upper troposphere). Accordingly, the rise of condensation kernels concentration (of water vapour) occurs, and, as a result, the transparency decrease of an atmosphere concerning infrared radiation of the Earth takes place. For checkout of the hypothesis the simplified physical-statistical model of energy-balance of atmosphere and hydrosphere is constructed. The modelling of the global temperatures and the temperature in the different regions of the North hemisphere was made. The effective coefficients of the model take into account, in particular, the features of ocean and atmosphere circulation. They were determined with use of step-by-step procedure of correction based on a Monte-Carlo method, under condition of achievement of the greatest correspondence between simulated and actual values of global temperature. The constructed model allows to explain (without attraction of the anthropogenous factor) modern global warming, and also known data about temperature oscillations during second millennium. The comparative analysis of model results with independently reconstructed regional temperatures, including Central England (1000-1659 years), China (1000-1990 years), Gulf of Alaska and Pacific Northwest (1752-1983 years), shows their good enough correspondence which allows to assume, that the solar activity oscillations really can be the main climate-forming factor in millennium scale. Moreover, there had been detected the close correlation between the rate of increase of CO2 concentration and the simulated temperature of the upper layer of

  7. 75 FR 35824 - Agency Information Collection Activities: Forms I-600/I-600A, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Forms I-600/I-600A, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Forms I- 600/I-600A, Petition To Classify Orphan as...

  8. 77 FR 64388 - Agency Information Collection (Former POW Medical History), VA Form 10-0048 Activities Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF VETERANS AFFAIRS Agency Information Collection (Former POW Medical History), VA Form 10-0048 Activities Under OMB....'' SUPPLEMENTARY INFORMATION: Title: Former POW Medical History, VA Form 10-0048. OMB Control Number:...

  9. Dissociation of membrane binding and lytic activities of the lymphocyte pore-forming protein (perforin).

    PubMed

    Young, J D; Damiano, A; DiNome, M A; Leong, L G; Cohn, Z A

    1987-05-01

    Granules isolated from CTL and NK cells contain a cytolytic pore-forming protein (PFP/perforin). At low temperatures (on ice), PFP binds to erythrocyte membranes without producing hemolysis. Hemolysis occurs when the PFP-bound erythrocytes are warmed up to 37 degrees C, which defines a temperature-dependent, lytic (pore-formation) step distinct from the membrane-binding event. Ca2+ and neutral pH are required for both membrane binding and pore formation by PFP. Serum, LDL, HDL, and heparin inhibit the hemolytic activity of PFP by blocking its binding to lipid membranes. Lysis by PFP that has bound to erythrocyte membranes is no longer susceptible to the effect of these inhibitors. The hemolytic activities associated with intact granules and solubilized PFP show different requirements for Ca2+ and pH, indicating that cytolysis produced by isolated granules may involve an additional step, possibly fusion of granules with membranes. It is suggested that three distinct Ca2+- and pH-dependent events may be involved during cell killing by CTL and NK cells: fusion of cytoplasmic granules of effector cells with their plasma membrane, releasing PFP from cells; binding of the released PFP to target membranes; and insertion of monomers and the subsequent formation of lytic pores in the target membrane. The serum-mediated inhibition of membrane binding by PFP could prevent the accidental injury of bystander cells by cell-released PFP, but would allow cytolysis to proceed to completion once PFP has bound to the target membrane. PMID:3494808

  10. Anion permeation in calcium-activated chloride channels formed by TMEM16A from Xenopus tropicalis.

    PubMed

    Reyes, J P; López-Rodríguez, A; Espino-Saldaña, A E; Huanosta-Gutiérrez, A; Miledi, R; Martínez-Torres, A

    2014-09-01

    Calcium-activated chloride channels (CaCC) formed by anoctamin1/TMEM16A subunits are ubiquitously expressed, and these channels are known to prevent polyspermy in amphibian oocytes. Here, we describe a TMEM16A clone isolated from Xenopus tropicalis oocytes (xtTMEM16A) and how the anion permeation properties are modified in single-site mutants of the ion pore. The anion permeability sequence was SCN(-) > I(-) > Br(-) > Cl(-) > gluconate (relative permeabilities 5.6:3.0:2.1:1:0.2, respectively). Dose-response curves indicated that the voltage-dependent half-maximal concentration for Ca(2+) activation (K d of the Hill equation at +100 mV) was 120 nM in normal external Cl(-), whereas it was displaced leftward to 75 nM Ca(2+), when I(-) replaced Cl(-). The I(-):Cl(-) mole fraction (MF) of the external solution was varied in order to gain insight into the permeation mechanism of the pore. No anomaly in MF behavior was observed for conductance, but it was observed for current reversal potential, which deviated from the prediction of the Goldman-Hodgkin-Katz equation. Mutations of positively charged amino acids in the pore, R646 and R761, to glutamate resulted in reduction of the relative permeability to I(-). Data from the wild type and mutants could be well fitted by a three-barrier, two-site permeation model. This suggests a multi-ion pore with at least two binding sites for anions, with R646 mole fraction closer to the extracellular membrane surface--being important for the stability of both sites--and R761--located deeper within the membrane--mainly affecting the innermost binding site. Considerations of xtTMEM16A putative pore region topology are discussed in the light of two alternative topological models of the protein. PMID:24352628

  11. 76 FR 51997 - Agency Information Collection Activities: Form I-602; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... Collection Under Review: Form I- 602, Application by Refugee for Waiver of Grounds of Excludability. The... the Form/Collection: Application by Refugee for Waiver of Grounds of Excludability. (3) Agency...

  12. 76 FR 24908 - Agency Information Collection Activities: Form I-693, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-03

    ... Information Collection Under Review: Form I- 693, Report of Medical Examination and Vaccination Record, OMB... Vaccination Record. (3) Agency form number, if any, and the applicable component of the Department of...

  13. 77 FR 71477 - Reports, Forms, and Recordkeeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-30

    ...-day comment period was published on June 13, 2012 (77 FR 35473-35475). DATES: Submit comments to the... collection of information. OMB Control Number: 2127-0665. NHTSA Form Number: NHTSA Form 1084. Abstract:...

  14. 78 FR 36632 - Reports, Forms, and Recordkeeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-18

    .... 77, No. 144/FR pp.35473-35475). DATES: Submit comments to the Office of Management and Budget (OMB.... Title: NHTSA Distracted Driving Survey Project. Form No.: NHTSA Form 1084. Type of Review: Revision...

  15. 75 FR 20033 - Reports, Forms and Recordkeeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-16

    .... Affected Public: Masters, officers and crewmembers of U.S. ships. Form(s): None. Abstract: This collection of information provides a method of awarding merchant marine medals and decorations to...

  16. 77 FR 67018 - Agency Information Collection Activities: Notice of Naturalization Oath Ceremony, Form Number N...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-08

    ... Naturalization Oath Ceremony, Form Number N-445; Extension, Without Change, of a Currently Approved Collection... Naturalization Oath Ceremony. (3) Agency form number, if any, and the applicable component of the DHS...

  17. A Review of Recent RTO Benefit-Cost Studies: Toward MoreComprehensive Assessments of FERC Electricity RestructuringPolicies

    SciTech Connect

    Eto, Joseph H.; Lesieutre, Bernard C.

    2005-12-01

    During the past three years, government and private organizations have issued more than a dozen studies of the benefits and costs of Regional Transmission Organizations (RTOs). Most of these studies have focused on benefits that can be readily estimated using traditional production-cost simulation techniques, which compare the cost of centralized dispatch under an RTO to dispatch in the absence of an RTO, and on costs associated with RTO start-up and operation. Taken as a whole, it is difficult to draw definitive conclusions from these studies because they have not examined potentially much larger benefits (and costs) resulting from the impacts of RTOs on reliability management, generation and transmission investment and operation, and wholesale electricity market operation. This report: (1) Describes the history of benefit-cost analysis of FERC electricity restructuring policies; (2)Reviews current practice by analyzing 11 RTO benefit-cost studies that were published between 2002 and 2004 and makes recommendations to improve the documentation of data and methods and the presentation of findings in future studies that focus primarily on estimating short-run economic impacts; and (3) Reviews important impacts of FERC policies that have been overlooked or incompletely treated by recent RTO benefit-cost studies and the challenges to crafting more comprehensive assessments of these impacts based on actual performance, including impacts on reliability management, generation and transmission investment and operation, and wholesale electricity market operation.

  18. 75 FR 11192 - Agency Information Collection Activities: Form I-730, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-10

    ... Information Collection under Review: Form I- 730, Refugee/Asylee Relative Petition. The Department of Homeland... collection. (2) Title of the Form/Collection: Refugee/Asylee Relative Petition. (3) Agency form number, if... used by an asylee or refugee to file on behalf of his or her spouse and/or children provided that...

  19. 75 FR 5101 - Agency Information Collection Activities: Form I-590, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... was previously published in the Federal Register onOctober 16, 2009, at 74 FR 53285, allowing for a 60... information collection under review: Form I- 590, Registratiod for Classification as Refugee; OMB Control No...) Title of the Form/Collection: Registration for Classification as Refugee. (3) Agency form number, if...

  20. 75 FR 31460 - Agency Information Collection Activities: Form I-730, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... previously published in the Federal Register on March 10, 2010 at 75 FR 11192, allowing for a 60- day public... Information Collection Under Review: Form I- 730, Refugee/Asylee Relative Petition. OMB Control No. 1615-0037... the Form/Collection: Refugee/Asylee Relative Petition. (3) Agency form number, if any, and...