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Sample records for activities ferc form

  1. 78 FR 45919 - Commission Information Collection Activities (FERC Form 80); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ... the Federal Register (78 FR 28820, 5/16/2013) requesting public comments. FERC received no comments on... Energy Regulatory Commission Commission Information Collection Activities (FERC Form 80); Comment Request... the requirements of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507(a)(1)(D), the Federal...

  2. 76 FR 46781 - Commission Information Collection Activities (FERC Form 1 and FERC Form 1F); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... to providing this information, such as administrative costs and the cost for information technology... 5 1 116 580 Total 243,438 The total estimated annual cost burden to respondents on the FERC Form 1 is $12,385,758 (242,858 hours x $51/hour \\3\\). The average cost per respondent is $59,262. \\3\\...

  3. 78 FR 26766 - Commission Information Collection Activities (FERC Form 580); Comment Request; Revision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-08

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC Form 580); Comment Request; Revision AGENCY: Federal Energy Regulatory Commission. ACTION: Notice of information...

  4. 75 FR 35776 - Commission Information Collection Activities (FERC Form No. 80); Comment Request; Submitted for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... the Federal Register (75 FR 19630, 4/ 15/2010) requesting public comments. FERC received no comments... Commission's regional office staff uses the FERC Form 80 data when conducting inspections of licensed... are contained in the Commission's regulations, FERC plans to submit FERC Form 80 to the Office...

  5. 78 FR 28820 - Commission Information Collection Activities (FERC Form 80); Comment Request; Revision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ... same docket, which was subsequently published in the Federal Register on 4/25/2013 (78 FR 24402). SUMMARY: In compliance with the requirements of the Paperwork Reduction Act of 1995, 44 U.S.C. 3506(c)(2... Energy Regulatory Commission Commission Information Collection Activities (FERC Form 80); Comment...

  6. 78 FR 24402 - Commission Information Collection Activities (FERC Form 80); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-25

    ... instructions, form, and glossary and did not attach those to this notice. Type of Respondents: Hydropower.... Bose, Secretary. [NOTE: The remainder of the FERC Form 80 (instructions, form, and glossary) is... understanding of terms, please refer to the Glossary. c. Report actual data for each item. If actual data...

  7. 78 FR 24187 - Commission Information Collection Activities (FERC Form 6-Q); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... issued a Notice in the Federal Register (78 FR 8500, 2/6/2013) requesting public comments. FERC received... Commerce Act (ICA) \\1\\, the Commission is authorized and empowered to make investigations and to collect... out the provisions of the ICA. FERC must ensure just and reasonable rates for transportation of...

  8. 75 FR 35003 - Commission Information Collection Activities (FERC Form No. 580); Request; Submitted for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... the Federal Register (74 FR 66114, 12/14/2009) requesting public comments. FERC received comments from...; Submitted for OMB Review June 15, 2010. AGENCY: Federal Energy Regulatory Commission. ACTION:...

  9. 75 FR 21623 - Commission Information Collection Activities (FERC Form Nos. 6 and 6-Q); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-26

    ... associated public costs follow. 1 2 \\1\\ These figures may not be exact, due to rounding and/or truncating. \\2... hours/year, the estimated cost for 1 full-time employee is $137,874/year. The estimated hourly cost is $66.29 (or $137,874/2,080). Estimated Estimated total annual FERC data collection Total annual...

  10. 75 FR 5066 - Commission Information Collection Activities (FERC Form 60,1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ...In compliance with the requirements of section 3507 of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507, the Federal Energy Regulatory Commission (Commission or FERC) has submitted the information collections described below to the Office of Management and Budget (OMB) for review of the information collection requirements. Any interested person may file comments directly with OMB and should......

  11. 78 FR 8500 - Commission Information Collection Activities (FERC Form 6-Q); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... changes to the current reporting requirements. Abstract: Under the Interstate Commerce Act (ICA),\\1\\ the... FERC may consider to be necessary or useful for the purpose of carrying out the provisions of the ICA... provisions of the ICA to include the authority to prescribe rules and regulations concerning...

  12. 78 FR 9679 - Commission Information Collection Activities (FERC-60, FERC-61, & FERC-555A); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-11

    ... Description of Service Company Functions), and FERC- 555A (Preservation of Records Companies and Service... Register (70 FR 70996, 11/28/2012) requesting public comments. FERC received no comments on the FERC-60... Service Companies), FERC-61 (Narrative Description of Service Company Functions), and FERC-...

  13. 75 FR 19630 - Commission Information Collection Activities (FERC Form 80); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ... title of Schedule 1 are attached. The remainder of the form, instructions, and glossary remain unchanged... of terms, please refer to the Glossary. c. Report actual data for each item. If actual data are..., instructions, and glossary) are unchanged and are not included here.] BILLING CODE 6717-01-P...

  14. 75 FR 2124 - Commission Information Collection Activities (FERC-538, FERC-539, FERC-577, FERC-606, and FERC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-14

    ..., such as administrative costs and the cost for information technology. Indirect or overhead costs are... burdens and the associated public costs follow. Number of Projected average FERC Data collection Projected... rounding and/or truncating. The total annual cost to respondents 1 2 is estimated as follows....

  15. 75 FR 15424 - Commission Information Collection Activities (FERC-538, FERC-539, FERC-577, FERC-606, and FERC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... issued a Notice in the Federal Register (75 FR 2124, 1/ 14/2010) requesting public comments. FERC... not be exact, due to rounding and/or truncating. Projected Projected Number of average burden Total... $137,874/year. The estimated hourly cost is $66.29 (or $137,874/2,080). Estimated total Total...

  16. 75 FR 5061 - Commission Information Collection Activities (FERC Form Nos. 6 and 6-Q); Comment Request; Extensions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... distinct. Under the Interstate Commerce Act (ICA), (49 U.S.C. 1, 20, 54 Stat. 916), the Commission is... consider to be necessary or useful for the purpose of carrying out the provisions of the ICA. FERC must... carry out its responsibilities in implementing the statutory provisions of the ICA, including...

  17. 78 FR 25431 - Revised Information Collection Activities (FERC-545, FERC-549, and FERC-550); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... issued a Notice in the Federal Register, 78 FR 10,614 (February 14, 2013), requesting public comments by... compliance with this requirement was included in Order No. 770, 77 FR 71,288 (Nov. 30, 2012), FERC Stats... oil pipelines. These procedures will replace the use of random-number generated passwords...

  18. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative description of service company functions. 366.23 Section 366.23 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS...

  19. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative description of service company functions. 366.23 Section 366.23 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS...

  20. 77 FR 8245 - Commission Information Collection Activities (FERC-65, FERC-65A, and FERC-65B); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-14

    ... Control Number. This should simplify the FERC administrative burden of processing these information... Notification 0 1 0 1 0 Total \\3\\ N/A \\3\\ N/A 9 \\3\\ N/A 25 The total estimated annual cost burden to respondents... employee. The estimated annual cost of filing the FERC-65, FERC-65A, and FERC-65B per response is...

  1. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 6-Q... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  2. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 6-Q... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  3. 78 FR 44424 - Revisions to Page 700 of FERC Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-24

    ... rate base, and income taxes reported on Page 700. \\8\\ Revisions to Page 700 of FERC Form No. 6, 77 FR... (Commission) is modifying Page 700 of FERC Form No. 6 (Form 6) to facilitate the calculation of an oil.... NOPR 22 2. Comments 24 3. Commission Determination 26 E. Calculation of Actual Rate of Return on...

  4. 75 FR 49914 - Commission Information Collection Activities (FERC-511 and FERC-515); Comment Request; Extensions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-16

    ... administrative costs and the cost for information technology. Indirect or overhead costs are costs incurred by an... 23 1 40 920 FERC-515 10 1 80 800 Total annual costs for these collections are estimated as: Cost burden per Total cost burden to FERC data collection respondent respondents \\5\\ FERC-511 $2,651...

  5. 75 FR 51027 - Commission Information Collection Activities (FERC-917 and FERC-918) 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... issued a Notice in the Federal Register (75 FR 30008, 5/ 28/2010) requesting public comments. FERC...; Recovery of Stranded Costs by Public Utilities and Transmitting Utilities, Order No. 888, 61 FR 21540 (May 10, 1996), FERC Stats. & Regs. ] 31,036 (1996), order on reh'g, Order No. 888-A, 62 FR 12274 (Mar....

  6. 78 FR 64484 - Commission Information Collection Activities (FERC-511 and FERC-515); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-29

    ...-511 at 78 FR 49490, 8/14/2013, and for FERC- 515 at 78 FR 49489, 8/14/2013) requesting public comments... FERC-511 to implement the statutory provisions of Sections 4(e) and 8 of the Federal Power Act (FPA).\\2...\\ Section 8 of the FPA provides that the voluntary transfer of any license is made only with the...

  7. 76 FR 28760 - Commission Information Collection Activities (FERC-73); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-18

    ... FR 12090, 2/ 24/2011) requesting public comments. FERC received one comment on the FERC-73 and has... Order No. 12009, 42 FR 46277 (September 13, 1977). The Commission has authority over interstate oil.... IC11- 73-001. Users interested in receiving automatic notification of activity in FERC Docket...

  8. 75 FR 70726 - Commission Information Collection Activities (FERC-511 and FERC-515); Comment Request; Submitted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-18

    ...In compliance with the requirements of section 3507 of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507, the Federal Energy Regulatory Commission (Commission or FERC) has submitted the information collections described below to the Office of Management and Budget (OMB) for review of the information collections requirements. Any interested person may file comments directly with OMB and......

  9. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Quarterly report of oil pipeline companies. 357.4 Section 357.4 Conservation of Power and Water Resources... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  10. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... submitting pages 301 and 700, each exempt oil pipeline carrier must include page 1 of Form No. 6, the...,” of FERC Form No. 6. When submitting page 700, each exempt oil pipeline carrier must include page 1...

  11. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Quarterly report of oil pipeline companies. 357.4 Section 357.4 Conservation of Power and Water Resources... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  12. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... submitting pages 301 and 700, each exempt oil pipeline carrier must include page 1 of Form No. 6, the...,” of FERC Form No. 6. When submitting page 700, each exempt oil pipeline carrier must include page 1...

  13. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... submitting pages 301 and 700, each exempt oil pipeline carrier must include page 1 of Form No. 6, the...,” of FERC Form No. 6. When submitting page 700, each exempt oil pipeline carrier must include page 1...

  14. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... submitting pages 301 and 700, each exempt oil pipeline carrier must include page 1 of Form No. 6, the...,” of FERC Form No. 6. When submitting page 700, each exempt oil pipeline carrier must include page 1...

  15. 18 CFR 357.2 - FERC Form No. 6, Annual Report of Oil Pipeline Companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... 6, Annual Report of Oil Pipeline Companies. (a) Who must file. (1) Each pipeline carrier subject to... submitting pages 301 and 700, each exempt oil pipeline carrier must include page 1 of Form No. 6, the...,” of FERC Form No. 6. When submitting page 700, each exempt oil pipeline carrier must include page 1...

  16. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Quarterly report of oil pipeline companies. 357.4 Section 357.4 Conservation of Power and Water Resources... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the...

  17. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 715, Annual Transmission Planning and Evaluation Report. 141.300 Section 141.300 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY REGULATORY POLICIES...

  18. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  19. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  20. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  1. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  2. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  3. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  4. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  5. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions....

  6. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies....

  7. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false FERC Form No. 73, Oil... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  8. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 73, Oil... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  9. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false FERC Form No. 73, Oil... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  10. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false FERC Form No. 73, Oil... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... Service life data is reported to the Commission by an oil pipeline company, as necessary,...

  11. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to..., Annual Transmission Planning and Evaluation Report. 141.300 Section 141.300 Conservation of Power and... FERC Form No. 715, Annual Transmission Planning and Evaluation Report. (a) Who must file....

  12. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 552, Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water... natural gas market participant, i.e., any buyer or seller that engaged in physical natural...

  13. 77 FR 59348 - Revisions to Page 700 of FERC Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-27

    ... National Environmental Policy Act, 52 FR 47897 (Dec. 17, 1987), FERC Stats. & Regs. ] 30,783 (1987). \\27... calculation of a pipeline's actual return on equity. The Commission proposes to expand the information... Pipeline Companies (Form 6), to facilitate the calculation of a pipeline's actual rate of return on...

  14. 18 CFR 369.1 - FERC Form No. 60, Annual report of centralized service company.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 60, Annual report of centralized service company. 369.1 Section 369.1 Conservation of Power and Water... HOLDING COMPANY ACT OF 2005, FEDERAL POWER ACT AND NATURAL GAS ACT STATEMENTS AND REPORTS...

  15. 18 CFR 369.1 - FERC Form No. 60, Annual report of centralized service company.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false FERC Form No. 60, Annual report of centralized service company. 369.1 Section 369.1 Conservation of Power and Water... HOLDING COMPANY ACT OF 2005, FEDERAL POWER ACT AND NATURAL GAS ACT STATEMENTS AND REPORTS...

  16. 18 CFR 369.1 - FERC Form No. 60, Annual report of centralized service company.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false FERC Form No. 60, Annual report of centralized service company. 369.1 Section 369.1 Conservation of Power and Water... HOLDING COMPANY ACT OF 2005, FEDERAL POWER ACT AND NATURAL GAS ACT STATEMENTS AND REPORTS...

  17. 18 CFR 369.1 - FERC Form No. 60, Annual report of centralized service company.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 60, Annual report of centralized service company. 369.1 Section 369.1 Conservation of Power and Water... HOLDING COMPANY ACT OF 2005, FEDERAL POWER ACT AND NATURAL GAS ACT STATEMENTS AND REPORTS...

  18. 18 CFR 369.1 - FERC Form No. 60, Annual report of centralized service company.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false FERC Form No. 60, Annual report of centralized service company. 369.1 Section 369.1 Conservation of Power and Water... HOLDING COMPANY ACT OF 2005, FEDERAL POWER ACT AND NATURAL GAS ACT STATEMENTS AND REPORTS...

  19. 77 FR 46086 - Commission Information Collection Activities (FERC-576); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-02

    ... Register (77 FR 28369, May 14, 2012) requesting public comments. FERC received no comments on the FERC-576...-8659 for TTY. Docket: Users interested in receiving automatic notification of activity in this docket... Commission of: (1) Damage to jurisdictional natural gas facilities as a result of a hurricane, earthquake,...

  20. 78 FR 16485 - Commission Information Collection Activities (FERC-607); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... issued a Notice in the Federal Register (77 FR 76015, December 26, 2012) requesting public comments. FERC... (EPAct 2005) modified FERC's role in order to better coordinate the activities of the separate agencies with varying responsibilities over proposed natural gas projects. Section 313 of EPAct 2005 \\1\\...

  1. 75 FR 7466 - Commission Information Collection Activities (FERC-912 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-19

    ... Docket No. IC09-912-001 requesting public comment (74 FR 63743, 12/4/ 2009), FERC and OMB received one... will be addressed in Docket No. RM09-23. FOR FURTHER INFORMATION: Ellen Brown may be reached by telephone at (202) 502-8663, by fax at (202) 273-0873, and by e-mail at ellen.brown@ferc.gov . Kimberly...

  2. 76 FR 66920 - Commission Information Collection Activities (FERC-549); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC-549); Comment Request; Submitted for OMB Review AGENCY: Federal Energy Regulatory Commission. ACTION: Notice. SUMMARY:...

  3. 77 FR 77069 - Commission Information Collection Activities (FERC-730); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    .../2006), published: 71 FR 43294. Actual transmission investment for the most recent calendar year, and... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Commission Information Collection Activities (FERC-730); Comment...

  4. 77 FR 76015 - Commission Information Collection Activities (FERC-607); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-26

    ... (EPAct 2005) modified FERC's role in order to better coordinate the activities of the separate agencies with varying responsibilities over proposed natural gas projects. Section 313 of EPAct 2005 \\1\\...

  5. 18 CFR 141.2 - FERC Form No. 1-F, Annual report for Nonmajor public utilities and licensees.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual report for Nonmajor public utilities and licensees. 141.2 Section 141.2 Conservation of Power and... ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978 STATEMENTS AND REPORTS (SCHEDULES) § 141.2 FERC Form No. 1-F, Annual report for Nonmajor public utilities and licensees. (a) Prescription. The form...

  6. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  7. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  8. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  9. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  10. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  11. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  12. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  13. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  14. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a)...

  15. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural...

  16. 18 CFR 141.1 - FERC Form No. 1, Annual report of Major electric utilities, licensees and others.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 1, Annual report of Major electric utilities, licensees and others. 141.1 Section 141.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL...

  17. 18 CFR 141.1 - FERC Form No. 1, Annual report of Major electric utilities, licensees and others.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false FERC Form No. 1, Annual report of Major electric utilities, licensees and others. 141.1 Section 141.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL...

  18. 18 CFR 141.1 - FERC Form No. 1, Annual report of Major electric utilities, licensees and others.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false FERC Form No. 1, Annual report of Major electric utilities, licensees and others. 141.1 Section 141.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL...

  19. 18 CFR 141.1 - FERC Form No. 1, Annual report of Major electric utilities, licensees and others.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false FERC Form No. 1, Annual report of Major electric utilities, licensees and others. 141.1 Section 141.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL...

  20. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric...

  1. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric...

  2. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric...

  3. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric...

  4. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  5. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  6. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  7. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  8. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the...

  9. 77 FR 69614 - Commission Information Collection Activities (FERC-714); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-20

    ... the Federal Register (77 FR 53878, 9/4/2012) requesting public comments. FERC received no comments on... Commission (Commission or FERC) is submitting the information collection FERC-714 (Annual Electric Balancing...: Title: FERC-714, Annual Electric Balancing Authority Area and Planning Area Report. OMB Control...

  10. 75 FR 71097 - Commission Information Collection Activities (FERC-919); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... Public Utilities, Order No. 697, 72 FR 39,904 (Jul. 20, 2007), FERC Stats. & Regs. ] 31,252 (2007), clarified, 121 FERC ] 61,260 (2007), order on reh'g, Order No. 697- A, 73 FR 25832 (May 7, 2008), FERC Stats. & Regs. ] 31,268, Order No. 697-B, 73 FR 79610 (Dec. 30, 2008), FERC Stats. & Regs. ] 31,285...

  11. 77 FR 64495 - Commission Information Collection Activities (FERC-922); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-22

    ... issued a Notice in the Federal Register (77 FR 12832, 3/2/2012) requesting public comments. FERC received...Library system (at http://www.ferc.gov/docs-filing/elibrary.asp ) by searching under Docket No....

  12. 78 FR 66350 - Commission Information Collection Activities (FERC-574); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... FR 49491, 8/14/2013) requesting public comments. FERC received no comments on the FERC-574 and is... Commission; and (d) that such State Commission is exercising that jurisdiction. 18 CFR Part 152 specifies...

  13. 76 FR 52647 - Commission Information Collection Activities (FERC-583); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... FR 34689, 06/14/2011) requesting public comments. FERC received no comments on the FERC-583 and is... government administrative costs and for the use of government dams. The Commission implements theses...

  14. 78 FR 36176 - Commission Information Collection Activities (FERC-551); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-17

    ...). FERC implemented Order Nos. 720 and 720-A to comply with the Energy Policy Act of 2005 (``EPAct 2005'') and specifically Section 23 of EPAct 2005, which amended the NGA to direct FERC to ``facilitate...

  15. 77 FR 76016 - Commission Information Collection Activities (FERC-606); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-26

    ... responsibilities over proposed natural gas projects, the Energy Policy Act of 2005 (EPAct 2005) modified FERC's role. Section 313 of EPAct 2005 \\1\\ directs FERC: (1) to establish a schedule for agencies to...

  16. 78 FR 7767 - Commission Information Collection Activities (FERC-729); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-04

    ... FR 67638, 11/13/2012) requesting public comments. FERC received no comments on the FERC-729 and is... collection implements the Commission's mandates under EPAct 2005 Section 1221 which authorizes the...

  17. 78 FR 16487 - Commission Information Collection Activities (FERC-556); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... facilities which make use of reject heat associated with commercial or industrial processes, and by small... FR 77067, 12/31/2012) requesting public comments. FERC received no comments on the FERC-556 and...

  18. 76 FR 54748 - Commission Information Collection Activities (FERC-519); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-02

    ... FR 35871, 6/ 20/2001) requesting public comments. FERC received no comments on the FERC-519 and has... requirements. Any interested person may file comments directly with OMB and should address a copy of those... filing formats are available at http://www.ferc.gov/help/submission-guide.asp . To file the...

  19. 76 FR 54749 - Commission Information Collection Activities (FERC-523); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-02

    ... FR 35870, 6/ 20/2011) requesting public comments. FERC received no comments on the FERC-523 and has... requirements. Any interested person may file comments directly with OMB and should address a copy of those... filing formats are available at http://www.ferc.gov/help/submission-guide.asp . To file the...

  20. 77 FR 69615 - Commission Information Collection Activities (FERC-715); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-20

    ... Register (77 FR 53877, 9/4/2012) requesting public comments. FERC received no comments on the FERC-715 and.... Additionally, the Office of Electric Reliability (OER) uses the FERC-715 data to help protect and improve the reliability and security of the nation's bulk power system. OER oversees the development and review...

  1. 76 FR 35872 - Commission Information Collection Activities (Ferc-603); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... FR 18743, 4/ 5/2011) requesting public comments. FERC received no comments on the FERC-603 and has... 21, 2003, the Commission issued Order No. 630 (66 FR 52917) to address the appropriate treatment of... requirements. Any interested person may file comments directly with OMB and should address a copy of...

  2. 76 FR 51960 - Commission Information Collection Activities (FERC-512); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... FR 30930, 05/27/2011) requesting public comments. FERC received no comments on the FERC-512 and has... requirements. Any interested person may file comments directly with OMB and should address a copy of those... should not make a paper filing. Commenters that are not able to file electronically must send an...

  3. 76 FR 19333 - Commission Information Collection Activities (FERC-725B); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-07

    ... requirements to safeguard critical cyber assets.\\2\\ These standards help protect the nation's Bulk-Power System against potential disruptions from cyber attacks.\\3\\ The CIP Reliability Standards include one actual... FR 65618, 10/26/2010) requesting public comments. FERC received no comments on the FERC-725B and...

  4. 77 FR 31342 - Commission Information Collection Activities (FERC-914); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-25

    ... Production and Cogeneration Facilities, Order No. 671, 71 FR 7852 (2/15/2006), FERC Stats. & Regs. ] 31,203...-A, 71 FR 30585 (5/30/2006), in Docket No. RM05-36. FERC implemented the Congressional mandate of the... interstate service, to order the rendering of adequate interstate service by public utilities, the rates...

  5. 78 FR 54880 - Commission Information Collection Activities (FERC-551); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-06

    ... Commission issued a Notice in the Federal Register (78 FR 36176, 6/ 17/2013) requesting public comments. FERC received no comments on the FERC-551 and is making this notation in its submittal to OMB. DATES: Comments..., the United States Court of Appeals for the Fifth Circuit issued a decision granting the Texas...

  6. 75 FR 61455 - Commission Information Collection Activities (FERC-582); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... the Federal Register (75 FR 44781, 07/29/2010) requesting public comments. FERC received comments from... power marketers subject to these annual charges must submit FERC-582 data to the Commission by April 30... and power marketers then must pay the charges within 45 days of the Commission's issuance of the...

  7. 77 FR 39691 - Commission Information Collection Activities (FERC-725); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... issued a Notice in the Federal Register (77 FR 24189, 04/23/2012) requesting public comments. FERC... ] 31,204 71 FR 8662 (2006) Order on rehearing, 71 FR 19,814 (2006), FERC Statutes and Regulations ] 31... @ $89.78/hr = $15,128,199. Regional Entity Cost: 593,120 hours @ $77.76/hr = $46,121,011....

  8. 77 FR 31607 - Commission Information Collection Activities (FERC-587); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... Notice in the Federal Register (77 FR 11518, 02/27/2012) requesting public comments. FERC received no...\\ Section 24 of the FPA requires that applicants proposing hydropower projects on (or changes to existing... of the hydropower proposal. FERC-587 consolidates the information required and identifies...

  9. 77 FR 28861 - Commission Information Collection Activities (FERC-585); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ..., Reporting of Electric Energy shortages and Contingency Plans under PURPA, to the Office of Management and... fax at (202) 273-0873. SUPPLEMENTARY INFORMATION: Title: FERC-585, Reporting of Electric Energy.... Abstract: The information collected under the requirements of FERC- 585, ``Reporting of Electric...

  10. 76 FR 18209 - Commission Information Collection Activities (FERC-725D); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... FR 71678, 11/24/2010) requesting public comments. FERC received no comments on the FERC-725D and has... Mandatory Reliability Standards for the Bulk-Power System, Order No. 693, 72 FR, 16,416 (April 4, 2007), 118... methodologies to determine system operating limits (SOLs) for the bulk- power system in the planning...

  11. 77 FR 16214 - Commission Information Collection Activities (FERC-598); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ... as explained below. The Commission issued a Notice in the Federal Register (77 FR 97, 1/3/2012... 2005, 70 FR. 75,592 (2005), FERC Statutes and Regulations ] 31,197 (2005) Order on reh'g, 71 FR 28,446 (2006), FERC Statutes and Regulations ] 31,213 (2006), order on reh'g, 71 FR 42,750 (2006),...

  12. 78 FR 16486 - Commission Information Collection Activities (FERC-606); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... explained below. The Commission issued a Notice in the Federal Register (77 FR 76016, 12/26/2012) requesting... varying responsibilities over proposed natural gas projects, the Energy Policy Act of 2005 (EPAct 2005) modified FERC's role. Section 313 of EPAct 2005 \\1\\ directs FERC to establish a schedule for agencies...

  13. 77 FR 71186 - Commission Information Collection Activities (FERC-912); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-29

    ... FR 58365, 09/20/2012) requesting public comments. FERC received no comments on the FERC-912 and is.... Abstract: On 8/8/2005, the Energy Policy Act of 2005 (EPAct 2005) \\1\\ was signed into law. Section 1253(a) of EPAct 2005 amends Section 210 of the Public Utility Regulatory Policies Act of 1978 (PURPA)...

  14. 78 FR 77111 - Commission Information Collection Activities (FERC-555); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-20

    ... FR 61983, 10/9/2013) requesting public comments. FERC received no comments on the FERC-555 and is... 20 of the Interstate Commerce Act (ICA).\\3\\ \\1\\ 16 U.S.C. 825, 825c and 825h. \\2\\ 15 U.S.C....

  15. 77 FR 35667 - Commission Information Collection Activities (FERC-567); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... Commission as explained below. The Commission issued a Notice in the Federal Register (77 FR 11520, 02/27... Regulations 1320.3. \\2\\ 77 FR 11520 (2/27/2012) FERC-567 (IC12-8-000): Gas Pipeline Certificates: Annual... collection FERC-567, Gas Pipeline Certificates: Annual Reports of System Flow Diagrams and System Capacity...

  16. 77 FR 3751 - Commission Information Collection Activities (FERC-725A); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ... issued a Notice in the Federal Register (76 FR 65504, 10/21/2011) requesting public comments. FERC.... Transmission owners. from R3 of FAC-008-1. R6 Generator owners and None, this requirement is derived...

  17. 75 FR 44781 - Commission Information Collection Activities (FERC-582); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-29

    ... Report.'' Public utilities and power marketers subject to these annual charges must submit FERC-582 data... charges, and public utilities and power marketers then must pay the charges within 45 days of...

  18. 77 FR 97 - Commission Information Collection Activities, Proposed Collection (FERC-598); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... Holding Company Act of 2005, 70 FR. 75,592 (2005), FERC Statutes and Regulations ] 31,197 (2005) Order on reh'g, 71 FR 28,446 (2006), FERC Statutes and Regulations ] 31,213 (2006), order on reh'g, 71 FR 42... statutory provisions of Title XII, subchapter F of the Energy Policy Act of 2005 (EPAct 2005).\\1\\ \\1\\...

  19. 77 FR 98 - Commission Information Collection Activities, Proposed Collection (FERC-716); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... cost estimate includes respondents' total salary and employment benefits. Comments are invited on: (1... CFR 2.20 identifies 12 components of a good faith estimate and 5 components of a reply to a good faith... total annual cost of filing FERC-716 is: 308 hours/2080 hours \\1\\ x $142,372 = $21,082.\\2\\ \\1\\ Number...

  20. 77 FR 53878 - Commission Information Collection Activities (FERC-714); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    ... comment on the currently approved information collection, Annual Electric Balancing Authority Area and...: FERC-714: Annual Electric Balancing Authority Area and Planning Area Report. OMB Control No.: 1902-0140... to collect information from electric utility balancing authorities and planning areas in the...

  1. 76 FR 31320 - Commission Information Collection Activities (FERC-725B); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... cyber attacks.\\4\\ The CIP Standards include one actual reporting requirement and several recordkeeping... (75 FR 65618, 10/26/2010) requesting public comments. In addition, FERC published a notice in the Federal Register (76 FR 19333, 4/7/2011) indicating submission to OMB of the information...

  2. 75 FR 34107 - Commission Information Collection Activities (FERC-547); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... administrative costs and the cost for information technology. Indirect or overhead costs are costs incurred by an... (1) (2) (3) (1) x (2) x (3) FERC-547 30 1 75 2,250 The total estimated annual cost burden to... information; and (7) transmitting or otherwise disclosing the information. The estimate of cost...

  3. 76 FR 30927 - Commission Information Collection Activities (FERC-516A), Proposed Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... administrative costs and the cost for information technology. Indirect or overhead costs are costs incurred by an... Interconnection Agreements and Procedures, Order No. 2006, 70 FR 34189 (May 12, 2005), FERC Stats. & Regs. ] 31...-conforming interconnection procedures and agreements that occur on occasion. The estimated total cost...

  4. 76 FR 30930 - Commission Information Collection Activities (FERC-512); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... attributable to providing this information, such as administrative costs and the cost for information... (2) x (3) FERC-512 200 1 37 7,400 Estimated cost burden to respondents is $508,000; [i.e., (7 hours... average annual cost burden per respondent is $2,540. The reporting burden includes the total time,...

  5. 75 FR 41840 - Commission Information Collection Activities (FERC-919); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... include all costs directly attributable to providing this information, such as administrative costs and... Public Utilities, Order No. 697, 72 FR 39,904 (Jul. 20, 2007), FERC Stats. & Regs. ] 31,252 (2007) (Final... presumption of horizontal market power, the Commission sets the just and reasonable rate at the default...

  6. 76 FR 30928 - Commision Information Collection Activities (FERC-588), Proposed Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ..., such as administrative costs and the cost for information technology. Indirect or overhead costs are... respondent response burden hours (1) (2) (3) (1) x (2) x (3) FERC-583 8 1 10 80 The estimated total cost to... salary per employee = $5,476 (rounded)). The estimated annual cost per respondent is $685 (rounded)....

  7. 75 FR 64301 - Commission Information Collection Activities (FERC-537); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-19

    ... to providing this information, such as administrative costs and the cost for information technology... presenting the basis for the proposed action; and Cost of the proposed facilities, plans for financing, and...) FERC-537 225 3.44 133 102,942 The total estimated annual cost burden to respondents is $6,823,570...

  8. 75 FR 47804 - Commission Information Collection Activities (FERC-574); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... administrative costs and the cost for information technology. Indirect or overhead costs are costs incurred by an...) response (3) burden hours (1)x(2)x(3) FERC-574 1 1 60 60 The total estimated annual cost burden to... an employee works each year. \\2\\ Estimated average annual cost per employee. The reporting...

  9. 78 FR 49490 - Commission Information Collection Activities (Ferc-511); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-14

    ... public comment on the currently approved information collection, FERC-511 (Transfer of Electric License... all license instruments, and evidence that such transfer is in the public interest. The Commission... foreign nations and among the several Sates, or upon any part of public lands and reservations of...

  10. 78 FR 43871 - Commission Information Collection Activities (FERC-580); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-22

    ... the Federal Register (78 FR 26766, 5/8/2013) requesting public comments. FERC received two comments on.... --Add a ``Copy Row'' button to facilitate data entry. We are further reducing the amount of information... and oil. --Add a ``Copy Contract'' button. Question 6a --Add a column labeled ``Is contract...

  11. 76 FR 49460 - Commission Information Collection Activities (FERC 516A); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-10

    ... transmission of electric energy in interstate commerce to amend their Open Access Transmission Tariffs (OATTs... Federal Register notice of May 27, 2011. (76 FR 30927) and has made this notation in its submission to OMB... downloaded remotely via the Internet through FERC's homepage using the ``eLibrary'' link. For user...

  12. 76 FR 4888 - Commission Information Collection Activities (FERC-537); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-27

    ...In compliance with the requirements of section 3507 of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507, the Federal Energy Regulatory Commission (Commission or FERC) has submitted the information collection described below to the Office of Management and Budget (OMB) for review of the information collection requirements. Any interested person may file comments directly with OMB and should......

  13. 76 FR 58803 - Commission Information Collection Activities (FERC-725A); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-22

    ...In compliance with the requirements of section 3507 of the Paperwork Reduction Act of 1995, 44 U.S.C. 3507, the Federal Energy Regulatory Commission (Commission or FERC) has submitted the information collection described below to the Office of Management and Budget (OMB) for review of the information collection requirements. Any interested person may file comments directly with OMB and should......

  14. 78 FR 53742 - Commission Information Collection Activities (Ferc-582); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-30

    ... the Federal Register (78 FR 47310, 8/5/2013) requesting public comments. FERC received no comments on... ``Estimate of Annual Burden'' section of this notice. \\1\\ 78 FR 30912, 5/23/2013; and 78 FR 47310, 8/5/2013... transmission of electric energy in interstate commerce. Respondents (public utilities, power marketers)...

  15. 77 FR 53877 - Commission Information Collection Activities (FERC-715); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    ... Electric Reliability (OER) uses the FERC-715 data to help protect and improve the reliability and security of the nation's bulk power system. OER oversees the development and review of mandatory reliability... operators of the bulk power system. OER also monitors and addresses issues concerning the nation's...

  16. 78 FR 62006 - Commission Information Collection Activities (FERC-919); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-11

    ... Public Utilities, Order No. 697, 72 FR 39904 (Jul. 20, 2007), FERC Stats. & Regs. ] 31,252 (2007) (Final... market power screens:The uncommitted pivotal supplier screen (which is based on the annual peak demand of the relevant market) and the uncommitted market share screen applied on a seasonal basis....

  17. 78 FR 47310 - Commission Information Collection Activities (FERC-582); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-05

    ... the Federal Register (78 FR 30912, 5/23/2013) requesting public comments. FERC received no comments on... information are due by September 4, 2013. ADDRESSES: Comments filed with OMB, identified by the OMB Control No...: Title: Electric Fees, Annual Charges, Waivers, and Exemptions. OMB Control No.: 1902-0132. Type...

  18. 77 FR 28863 - Commission Information Collection Activities (FERC-915); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ... utilities with approved market-based rate authority-- http://www.ferc.gov/industries/electric/gen-info/mbr... \\3\\ = $32,870. \\3\\ 2012 average hourly wage of filing clerk working within an electric utility. Record retention/storage cost (using an estimate of 48,891 cubic feet): $315,792.\\4\\ \\4\\ The...

  19. 75 FR 74696 - Commission Information Collection Activities (FERC-555); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-01

    ... issued a Notice in the Federal Register (75 FR 57744, 09/22/2010) requesting public comments. FERC... Act (NGA) (15 U.S.C. 717-717w), and section 20 of the Interstate Commerce Act (ICA, 49 U.S.C. 20)....

  20. 75 FR 5063 - Commission Information Collection Activities (FERC-729); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... comments in response to the Federal Register notice (74 FR 52796, 10/14/2009). FERC has summarized and... and impact of the facilities, early in the planning stages of the proposed facilities as required... stakeholder questions, do not plan for public stakeholder input, do not adequately explain...

  1. 75 FR 70219 - Commission Information Collection Activities (FERC-574); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ...: Federal Energy Regulatory Commission Desk Officer. Comments to Created by OMB should be filed electronically, c/o oira_submission@omb.eop.gov and include OMB Control Number 1902-0116 for reference. The Desk... instructions and acceptable filing formats are available at http://www.ferc.gov/help/submission-guide.asp ....

  2. 75 FR 56082 - Commission Information Collection Activities (FERC-725E); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-15

    ... the Federal Register (75 FR 35002, 6/ 21/2010) requesting public comments. FERC received no comments...).\\3\\ \\1\\ 16 U.S.C. 824o(e)(4). \\2\\ 16 U.S.C. 824o(a)(7) and (e)(4). \\3\\ 72 FR 33462, June 18, 2007... following: WECC-BAL-STD-002-0--balancing authorities and reserve sharing groups are to submit to...

  3. 77 FR 28369 - Commission Information Collection Activities (FERC-576); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-14

    ... facilities as a result of a hurricane, earthquake, or other natural disaster, or terrorist activity, (2... interested in receiving automatic notification of activity in this docket or in viewing/downloading comments... disaster or terrorist activity, that creates the potential for serious delivery problems on the...

  4. 78 FR 58296 - Commission Information Collection Activities (FERC-604); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-23

    ... Respondents: Public utilities and licensees, natural gas companies, and oil pipeline companies. Estimate of... burden A B (A) x (B) = (C) D (C) x (D) Public utilities and licensees, natural gas companies, and oil \\2... other forms of information technology. Dated: September 16, 2013. Kimberly D. Bose, Secretary....

  5. 77 FR 70746 - Commission Information Collection Activities (FERC-592); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-27

    ... Provider; and Marketing Affiliates of Interstate Pipelines. DATES: Comments on the collection of... Marketing Affiliates of Interstate Pipelines. OMB Control No.: 1 902-0157. Type of Request: Three-year... the pipeline's transportation, sales, and storage activities for its marketing affiliate to...

  6. 78 FR 11167 - Commission Information Collection Activities (FERC-592); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-15

    ... respondents to monitor the pipeline's transportation, sales, and storage activities for its marketing... Conduct for Transmission Provider; and Marketing Affiliates of Interstate Pipelines), to the Office of... explained below. The Commission issued a Notice in the Federal Register (77 FR 70746, 11/27/2012)...

  7. 18 CFR 131.80 - FERC Form No. 556, Certification of qualifying facility (QF) status for a small power production...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Certification of qualifying facility (QF) status for a small power production or cogeneration facility. 131.80 Section 131.80 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978...

  8. 77 FR 77067 - Commission Information Collection Activities (FERC Form No. 556); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ... of electric power by cogeneration facilities which make use of reject heat associated with commercial... energy and capacity from QFs favorably priced on the basis of the avoided cost of the power that is... the public avoided cost information and system capacity needs. 18 CFR Part 292 exempts QFs...

  9. 18 CFR 366.4 - FERC-65, notification of holding company status, FERC-65A, exemption notification, and FERC-65B...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... who file FERC-65A must include a form of notice suitable for publication in the Federal Register in... file a petition must include a form of notice suitable for publication in the Federal Register in...-65B must include a form of notice suitable for publication in the Federal Register in accordance...

  10. 18 CFR 366.4 - FERC-65, notification of holding company status, FERC-65A, exemption notification, and FERC-65B...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC-65, notification of holding company status, FERC-65A, exemption notification, and FERC-65B, waiver notification. 366.4 Section 366.4 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION,...

  11. FERC sees huge potential for demand response

    SciTech Connect

    2010-04-15

    The FERC study concludes that U.S. peak demand can be reduced by as much as 188 GW -- roughly 20 percent -- under the most aggressive scenario. More moderate -- and realistic -- scenarios produce smaller but still significant reductions in peak demand. The FERC report is quick to point out that these are estimates of the potential, not projections of what could actually be achieved. The main varieties of demand response programs include interruptible tariffs, direct load control (DLC), and a number of pricing schemes.

  12. 30 CFR 285.638 - What must I do upon completion of my commercial operations as approved in my COP or FERC license?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... operations as approved in my COP or FERC license? 285.638 Section 285.638 Mineral Resources BUREAU OF OCEAN... Activities Under An Approved Cop § 285.638 What must I do upon completion of my commercial operations as approved in my COP or FERC license? (a) Upon completion of your approved activities under your COP,...

  13. Test for abuse under NGPA Section 601(c)(2): Office of Consumers' Counsel v. FERC

    SciTech Connect

    Not Available

    1987-01-01

    A review of fraud and abuse challenge against the Natural Gas Policy Act (NGPA) section 601, which allows the Federal Energy Regulatory Commission to control the prices pipeline companies pay. The author explores the statutory background of the case, FERC's authority to interpret provisions of the NGPA in the area of take-or-pay problems, and court opinions. He concludes by outlining approaches for applying the fraud and abuse test, and views the guaranteed pass-through exception of fraud, abuse, or similar grounds as a valuable enforcement tool for FERC. As the terms become better defined, pipelines will be able to structure their business activities to avoid situations likely to lead to fraud or abuse charges. Both FERC and the natural gas industry will benefit from further development of the standard.

  14. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.8 System flow diagrams: Format...

  15. Active superconducting devices formed of thin films

    DOEpatents

    Martens, Jon S.; Beyer, James B.; Nordman, James E.; Hohenwarter, Gert K. G.

    1991-05-28

    Active superconducting devices are formed of thin films of superconductor which include a main conduction channel which has an active weak link region. The weak link region is composed of an array of links of thin film superconductor spaced from one another by voids and selected in size and thickness such that magnetic flux can propagate across the weak link region when it is superconducting. Magnetic flux applied to the weak link region will propagate across the array of links causing localized loss of superconductivity in the links and changing the effective resistance across the links. The magnetic flux can be applied from a control line formed of a superconducting film deposited coplanar with the main conduction channel and weak link region on a substrate. The devices can be formed of any type to superconductor but are particularly well suited to the high temperature superconductors since the devices can be entirely formed from coplanar films with no overlying regions. The devices can be utilized for a variety of electrical components, including switching circuits, amplifiers, oscillators and modulators, and are well suited to microwave frequency applications.

  16. Four olive branches: What the FERC bestowed on PUCs in Order 888

    SciTech Connect

    Hanger, J.R.

    1996-10-01

    Where others see conflict, a Pennsylvania commissioner finds a peace offering, not a grab for power. The jurisdictional issues posed by Order 888 continue to breed tension between federal and state officials. Unfortunately, most of this tension too often elevates form over substance. This jurisdictional tension shifts the focus of decisionmaking from securing the benefits of competition to preserving regulatory turf. In Order 888, the Federal Energy Regulatory Commission (FERC) extends four important olive branches to those states that want to introduce customer choice at the retail level. While these olive branches are not a fundamental concession by the FERC, state officials should pursue them before attacking Order 888 for its broad claims over jurisdiction.

  17. 76 FR 5799 - Notice of Commissioners and Staff Attendance at FERC Leadership Development Program Induction...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-02

    ... Energy Regulatory Commission Notice of Commissioners and Staff Attendance at FERC Leadership Development...: FERC Leadership Development Program Induction Ceremony: 888 First Street, NE., Washington, DC 20426... Leadership Development Program. Kimberly D. Bose, Secretary. BILLING CODE 6717-01-P...

  18. Multiple active forms of thrombin. IV. Relative activities of meizothrombins

    SciTech Connect

    Doyle, M.F.; Mann, K.G. )

    1990-06-25

    The prothrombin activation intermediates meizothrombin and meizothrombin(desF1) (meizothrombin that has been autoproteolyzed to remove fragment 1) have been obtained in a relatively pure, active form with minimal autolysis, making them suitable for enzymatic characterization. When compared at equimolar concentrations, alpha-thrombin, fragment 1.2+ alpha-thrombin, meizothrombin(desF1), and meizothrombin have approximately 100, 100, 10, and 1% activity, respectively, toward the macromolecular substrates factor V, fibrinogen, and platelets. The difference in activity of these four enzymes cannot be attributed to alterations in the catalytic triad, as all four enzymes have nearly identical catalytic efficiency toward the chromogenic substrate S2238. Further, the ability of meizothrombin and meizothrombin(desF1) to activate protein C was 75% of the activity exhibited by alpha-thrombin or fragment 1.2+ alpha-thrombin. All four enzymes bind to thrombomodulin, as judged by the enhanced rate of protein C activation upon preincubation of the enzymes with thrombomodulin. The extent of rate enhancement varied, with meizothrombin/thrombomodulin exhibiting only 50% of the alpha-thrombin/thrombomodulin rate. This difference in rate is not due to a decreased affinity of the meizothrombin for thrombomodulin since the apparent dissociation constants for the alpha-thrombin-thrombomodulin complex and the meizothrombin-thrombomodulin complex are virtually identical. The difference in the observed rate is due in part to the higher Km for protein C exhibited by the meizothrombin-thrombomodulin complex. Incubation of the thrombomodulin-enzyme complex with phospholipid vesicles caused an increase in the protein C activation rates. The kinetic constants for protein C activation in the presence of phospholipid are virtually identical for these enzyme-thrombomodulin complexes.

  19. 77 FR 69450 - Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-19

    ... Energy Regulatory Commission Notice of FERC Staff Attendance Docket No. PacifiCorp ER13-64-000 Deseret... staff will attend a meeting conducted by representatives of WestConnect, ColumbiaGrid, Northern Tier..., may be found at www.nttg.biz . The meeting is open to all stakeholders and Commission...

  20. 77 FR 66609 - Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ... Federal Energy Regulatory Commission Notice of FERC Staff Attendance October 31, 2012. Docket No. Pacifi... notice that on November 7, 2012, members of its staff will attend a meeting conducted by representatives... stakeholders and Commission staff's attendance is part of the Commission's ongoing outreach efforts....

  1. 77 FR 74844 - Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-18

    ... Energy Regulatory Commission Notice of FERC Staff Attendance PacifiCorp Docket No. ER13-64-000. Deseret... that on December 19, 2012, members of its staff will attend a meeting hosted by NV Energy, Inc... meeting is open to all stakeholders and Commission staff's attendance is part of the Commission's...

  2. 78 FR 16497 - Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... Energy Regulatory Commission Notice of FERC Staff Attendance Docket No. PacifiCorp ER13-64-000 Deseret... its staff will attend a conference call conducted by representatives of California Independent System... stakeholders and Commission staff's attendance is part of the Commission's ongoing outreach efforts....

  3. Implications of the Bangor Hydro decision on FERC relicensings

    USGS Publications Warehouse

    Pizzi, L.

    1997-01-01

    In the next 15 years, over 400 existing nonfederal dams will require new licenses by the Federal Energy Regulatory Commission (FERC) in order to continue operations. In many cases, state or federal agencies have a unique opportunity to condition the hydropower licenses to protect environmental values. In 1996, the Court of Appeals for the District of Columbia Circuit issued an opinion striking down a mandatory license condition imposed by the U.S. Department of the Interior in a FERC relicensing. The case, Bangor Hydro-Electric Company v.Federal Energy Regulatory Commission, is instructive for agencies regarding the procedures to be followed in imposing mandatory FERC licensing conditions in the future. After Bangor Hydro, agencies should: (1) support their decisions by substantial evidence in the FERC record, (2) request applicant studies to support agency decisions and allow public comment on the decisions as a means to test the facts and analysis, and (3) consider intervening in a case to defend the condition on appeal. Bangor Hydro also raises, but does not decide, the issue of whether the agency imposing the mandatory condition should engage in balancing the economics of the hydropower project with the public resources affected by the project.

  4. 75 FR 35001 - Commission Information Collection Activities (FERC-FERC-725E); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... Coordinating Council (WECC).\\3\\ \\1\\ 16 U.S.C. 824o(e)(4). \\2\\ 16 U.S.C. 824o(a)(7) and (e)(4). \\3\\ 72 FR 33462... balancing authorities and reserve sharing groups to submit to WECC quarterly reports on operating reserves... operators, balancing authorities and load-serving entities to document and report to WECC actions taken...

  5. 78 FR 69843 - Commission Information Collection Activities (Ferc-Ferc-549d); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-21

    ...) Implementation Burden PDF filings 3 1 3 68 204 XML filings 2 1 2 104 208 Ongoing Burden PDF filings 76 4 304 12.5 3,800 XML filings 33 4 132 10 1,320 TOTAL \\4\\ 109 109 5,532 \\4\\ This figure does not include...

  6. 76 FR 42129 - Agency Information Collection Activities: Case Submission Form, Case Assistance Form

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-18

    ... FY2010. We are requesting a two year approval for the form anticipating Government Paperwork Elimination... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Agency Information Collection Activities: Case Submission Form, Case Assistance Form (Form...

  7. Neural network with formed dynamics of activity

    SciTech Connect

    Dunin-Barkovskii, V.L.; Osovets, N.B.

    1995-03-01

    The problem of developing a neural network with a given pattern of the state sequence is considered. A neural network structure and an algorithm, of forming its bond matrix which lead to an approximate but robust solution of the problem are proposed and discussed. Limiting characteristics of the serviceability of the proposed structure are studied. Various methods of visualizing dynamic processes in a neural network are compared. Possible applications of the results obtained for interpretation of neurophysiological data and in neuroinformatics systems are discussed.

  8. 75 FR 26782 - Agency Information Collection Activities: Form I-864, Form I-864A, Form I-864EZ, and Form I-864W...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-864, Form I- 864A, Form I-864EZ, and Form I-864W; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form I- 864, Affidavit of...

  9. Silk microgels formed by proteolytic enzyme activity.

    PubMed

    Samal, Sangram K; Dash, Mamoni; Chiellini, Federica; Kaplan, David L; Chiellini, Emo

    2013-09-01

    The proteolytic enzyme α-chymotrypsin selectively cleaves the amorphous regions of silk fibroin protein (SFP) and allows the crystalline regions to self-assemble into silk microgels (SMGs) at physiological temperature. These microgels consist of lamellar crystals in the micrometer scale, in contrast to the nanometer-scaled crystals in native silkworm fibers. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zeta potential results demonstrated that α-chymotrypsin utilized only the non-amorphous domains or segments of the heavy chain of SFP to form negatively charged SMGs. The SMGs were characterized in terms of size, charge, structure, morphology, crystallinity, swelling kinetics, water content and thermal properties. The results suggest that the present technique of preparing SMGs by α-chymotrypsin is simple and efficient, and that the prepared SMGs have useful features for studies related to biomaterial and pharmaceutical needs. This process is also an easy way to obtain the amorphous peptide chains for further study. PMID:23756227

  10. 76 FR 61725 - Agency Information Collection Activities: Case Submission Form, Case Assistance Form; (Form DHS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... Federal Register on July 18, 2011 at 76 FR 42129, for a 60-day public comment period. No comments were... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND...-7001), Online Ombudsman Form DHS-7001 AGENCY: Office of the Citizenship and Immigration...

  11. Antioxidative activity of bound-form phenolics in potato peel.

    PubMed

    Nara, Kazuhiro; Miyoshi, Takayuki; Honma, Tamaki; Koga, Hidenori

    2006-06-01

    Free and bound-form phenolics were isolated from potato (cv. Toyoshiro) flesh and peel. The free and bound-form phenolics in the peel showed high DPPH radical scavenging activity, while those in the flesh showed low activity. The total amount of chlorogenic acid and caffeic acid in the free-form phenolics from the peel was highly correlated with the DPPH radical scavenging activity. Ferulic acid was identified as the active radical scavenging compound in the bound-form phenolics from the peel. The potato peel may therefore offer an effective source of an antioxidative. PMID:16794331

  12. 76 FR 41279 - Agency Information Collection Activities; Form I-864, Form I-864A, Form I-864EZ, and Form I-864W...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-13

    ... was previously published in the Federal Register on May 4, 2011, at 76 FR 25364, allowing for a 60-day... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities; Form I-864, Form I- 864A, Form I-864EZ, and Form I-864W; Extension of an Existing Information Collection;...

  13. 75 FR 51093 - Agency Information Collection Activities: Form I-864, Form I-864A, Form I-864EZ, and Form I-864W...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... on May 12, 2010, at 75 FR 26782, allowing for a 60-day public comment period. USCIS received 2... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-864, Form I- 864A, Form I-864EZ, and Form I-864W; Extension of a Currently Approved Information...

  14. Pu-238 fuel form activities, January 1-31, 1983

    SciTech Connect

    Not Available

    1983-03-01

    This monthly report for /sup 238/Pu Fuel Form Activities has two main sections: SRP-PuFF facility and SRL Fuel Form Activities. The program status, budget information, and milestone schedules are discussed in each main section. The Work Breakdown Structure (WBS) for this program is shown. Only one monthly report per year is processed for EDB.

  15. Pu-238 fuel form activities, January 1-31, 1981

    SciTech Connect

    Not Available

    1981-02-01

    This monthly report for /sup 238/Pu Fuel Form Activities has two main sections: SRP-PuFF facility and SRL Fuel Form Activities. The program status, budget information, and milestone schedules are discussed in each main section. The Work Breakdown Structure (WBS) for this program is shown. Only one monthly report per year is processed for EDB.

  16. Pu-238 fuel form activities, January 1-31, 1982

    SciTech Connect

    Not Available

    1982-03-01

    This monthly report for /sup 238/Pu fuel form activities has two main sections: SRP-PuFF facility and SRL fuel form activities. The program status, budget information, and milestone schedules are discussed in each main section. The Work Breakdown Structure (WBS) for this program is shown. Only one monthly report per year is processed for EDB.

  17. Form-Focused Discovery Activities in English Classes

    ERIC Educational Resources Information Center

    Ogeyik, Muhlise Cosgun

    2011-01-01

    Form-focused discovery activities allow language learners to grasp various aspects of a target language by contributing implicit knowledge by using discovered explicit knowledge. Moreover, such activities can assist learners to perceive and discover the features of their language input. In foreign language teaching environments, they can be used…

  18. 78 FR 11868 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-20

    ... Energy Market Regulation, Federal Energy Regulatory Commission at (317) 249- 5937 or patrick.clarey@ferc... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State...

  19. Activated carbon fibers and engineered forms from renewable resources

    DOEpatents

    Baker, Frederick S

    2013-02-19

    A method of producing activated carbon fibers (ACFs) includes the steps of providing a natural carbonaceous precursor fiber material, blending the carbonaceous precursor material with a chemical activation agent to form chemical agent-impregnated precursor fibers, spinning the chemical agent-impregnated precursor material into fibers, and thermally treating the chemical agent-impregnated precursor fibers. The carbonaceous precursor material is both carbonized and activated to form ACFs in a single step. The method produces ACFs exclusive of a step to isolate an intermediate carbon fiber.

  20. Activated carbon fibers and engineered forms from renewable resources

    DOEpatents

    Baker, Frederick S.

    2010-06-01

    A method of producing activated carbon fibers (ACFs) includes the steps of providing a natural carbonaceous precursor fiber material, blending the carbonaceous precursor material with a chemical activation agent to form chemical agent-impregnated precursor fibers, spinning the chemical agent-impregnated precursor material into fibers, and thermally treating the chemical agent-impregnated precursor fibers. The carbonaceous precursor material is both carbonized and activated to form ACFs in a single step. The method produces ACFs exclusive of a step to isolate an intermediate carbon fiber.

  1. Pore-forming activity of clostridial binary toxins.

    PubMed

    Knapp, O; Benz, R; Popoff, M R

    2016-03-01

    Clostridial binary toxins (Clostridium perfringens Iota toxin, Clostridium difficile transferase, Clostridium spiroforme toxin, Clostridium botulinum C2 toxin) as Bacillus binary toxins, including Bacillus anthracis toxins consist of two independent proteins, one being the binding component which mediates the internalization into cell of the intracellularly active component. Clostridial binary toxins induce actin cytoskeleton disorganization through mono-ADP-ribosylation of globular actin and are responsible for enteric diseases. Clostridial and Bacillus binary toxins share structurally and functionally related binding components which recognize specific cell receptors, oligomerize, form pores in endocytic vesicle membrane, and mediate the transport of the enzymatic component into the cytosol. Binding components retain the global structure of pore-forming toxins (PFTs) from the cholesterol-dependent cytotoxin family such as perfringolysin. However, their pore-forming activity notably that of clostridial binding components is more related to that of heptameric PFT family including aerolysin and C. perfringens epsilon toxin. This review focuses upon pore-forming activity of clostridial binary toxins compared to other related PFTs. This article is part of a Special Issue entitled: Pore-Forming Toxins edited by Mauro Dalla Serra and Franco Gambale. PMID:26278641

  2. 77 FR 58375 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-20

    ... information, contact Patrick Clarey, Office of Energy Market Regulation, Federal Energy Regulatory Commission... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State...

  3. 75 FR 53295 - Notice of FERC Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    .... For more information, contact Patrick Clarey, Office of Energy Market Regulation, Federal Energy... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Attendance at the Entergy Regional State Committee...

  4. 76 FR 29235 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-20

    ...] California Independent System Operator Corporation; Notice of FERC Staff Attendance The Federal Energy..., Market Update, Board of Governors Meeting, Settlement Process Timeline Change, CA Transmission Planning Group Call May 20, 2011, Congestion Revenue Rights Enhancements, Transmission Planning Standards May...

  5. Activated and unactivated forms of human erythrocyte aldose reductase.

    PubMed Central

    Srivastava, S K; Hair, G A; Das, B

    1985-01-01

    Aldose reductase (alditol:NADP+ 1-oxidoreductase, EC 1.1.1.21) has been partially purified from human erythrocytes by DEAE-cellulose (DE-52) column chromatography. This enzyme is activated severalfold upon incubation with 10 microM each glucose 6-phosphate, NADPH, and glucose. The activation of the enzyme was confirmed by following the oxidation of NADPH as well as the formation of sorbitol with glucose as substrate. The activated form of aldose reductase exhibited monophasic kinetics with both glyceraldehyde and glucose (Km of glucose = 0.68 mM and Km of glyceraldehyde = 0.096 mM), whereas the native (unactivated) enzyme exhibited biphasic kinetics (Km of glucose = 9.0 and 0.9 mM and Km of glyceraldehyde = 1.1 and 0.14 mM). The unactivated enzyme was strongly inhibited by aldose reductase inhibitors such as sorbinil, alrestatin, and quercetrin, and by phosphorylated intermediates such as ADP, glycerate 3-phosphate, glycerate 1,3-bisphosphate, and glycerate 2,3-trisphosphate. The activated form of the enzyme was less susceptible to inhibition by aldose reductase inhibitors and phosphorylated intermediates. PMID:3933003

  6. Channel-Forming Activities in the Glycosomal Fraction from the Bloodstream Form of Trypanosoma brucei

    PubMed Central

    Miinalainen, Ilkka J.; Hiltunen, J. Kalervo; Michels, Paul A. M.; Antonenkov, Vasily D.

    2012-01-01

    Background Glycosomes are a specialized form of peroxisomes (microbodies) present in unicellular eukaryotes that belong to the Kinetoplastea order, such as Trypanosoma and Leishmania species, parasitic protists causing severe diseases of livestock and humans in subtropical and tropical countries. The organelles harbour most enzymes of the glycolytic pathway that is responsible for substrate-level ATP production in the cell. Glycolysis is essential for bloodstream-form Trypanosoma brucei and enzymes comprising this pathway have been validated as drug targets. Glycosomes are surrounded by a single membrane. How glycolytic metabolites are transported across the glycosomal membrane is unclear. Methods/Principal Findings We hypothesized that glycosomal membrane, similarly to membranes of yeast and mammalian peroxisomes, contains channel-forming proteins involved in the selective transfer of metabolites. To verify this prediction, we isolated a glycosomal fraction from bloodstream-form T.brucei and reconstituted solubilized membrane proteins into planar lipid bilayers. The electrophysiological characteristics of the channels were studied using multiple channel recording and single channel analysis. Three main channel-forming activities were detected with current amplitudes 70–80 pA, 20–25 pA, and 8–11 pA, respectively (holding potential +10 mV and 3.0 M KCl as an electrolyte). All channels were in fully open state in a range of voltages ±150 mV and showed no sub-conductance transitions. The channel with current amplitude 20–25 pA is anion-selective (PK+/PCl−∼0.31), while the other two types of channels are slightly selective for cations (PK+/PCl− ratios ∼1.15 and ∼1.27 for the high- and low-conductance channels, respectively). The anion-selective channel showed an intrinsic current rectification that may suggest a functional asymmetry of the channel's pore. Conclusions/Significance These results indicate that the membrane of glycosomes apparently

  7. NUCLEAR ACTIVITY IS MORE PREVALENT IN STAR-FORMING GALAXIES

    SciTech Connect

    Rosario, D. J.; Lutz, D.; Berta, S.; Popesso, P.; Genzel, R.; Saintonge, A.; Tacconi, L.; Wuyts, S. E-mail: lutz@mpe.mpg.de E-mail: popesso@mpe.mpg.de E-mail: amelie@mpe.mpg.de E-mail: swuyts@mpe.mpg.de; and others

    2013-07-01

    We explore the question of whether low and moderate luminosity active galactic nuclei (AGNs) are preferentially found in galaxies that are undergoing a transition from active star formation (SF) to quiescence. This notion has been suggested by studies of the UV-optical colors of AGN hosts, which find them to be common among galaxies in the so-called Green Valley, a region of galaxy color space believed to be composed mostly of galaxies undergoing SF quenching. Combining the deepest current X-ray and Herschel/PACS far-infrared (FIR) observations of the two Chandra Deep Fields with redshifts, stellar masses, and rest-frame photometry derived from the extensive and uniform multi-wavelength data in these fields, we compare the rest-frame U - V color distributions and star formation rate distributions of AGNs and carefully constructed samples of inactive control galaxies. The UV-to-optical colors of AGNs are consistent with equally massive inactive galaxies at redshifts out to z {approx} 2, but we show that such colors are poor tracers of SF. While the FIR distributions of both star-forming AGNs and star-forming inactive galaxies are statistically similar, we show that AGNs are preferentially found in star-forming host galaxies, or, in other words, AGNs are less likely to be found in weakly star-forming or quenched galaxies. We postulate that, among X-ray-selected AGNs of low and moderate accretion luminosities, the supply of cold gas primarily determines the accretion rate distribution of the nuclear black holes.

  8. 76 FR 25364 - Agency Information Collection Activities: Form I-864, Form I-864A, Form I-864EZ, and From I-864W...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-04

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-864, Form I- 864A, Form I-864EZ, and From I-864W; Extension of an Existing Information Collection; Comment Request. ACTION: 60-Day Notice of Information Collection Under Review; Form I- 864, Affidavit of...

  9. Active curcumin nanoparticles formed from a volatile microemulsion template.

    PubMed

    Margulis, K; Srinivasan, S; Ware, M J; Summers, H D; Godin, B; Magdassi, S

    2014-01-01

    We report on biological performance of organic nanoparticles formed by a simple method based on rapid solvent removal from a volatile microemulsion. The particular focus of the study was on testing the suitability of the method for substances soluble in partially water-miscible organic solvents as well as on evaluating the therapeutic activity of the resultant nanoparticles. Curcumin was employed as a model for hydrophobic drug, and, as it is soluble in water-miscible organic solvents, it was successfully incorporated into a new cyclopentanone-water microemulsion system. During rapid solvent removal by spray-drying, the nanometric droplets of the microemulsion were converted into nanoparticles containing amorphous curcumin with the average size of 20.2±3.4 nm, having ζ potential of -36.2 ±1.8 mV. These nanoparticles were dispersible in water and retained the high loading of the active substance. The therapeutic activity of the resulting nanoparticles was demonstrated in a pancreatic cancer cell line Panc-1. The effective concentration for reducing the metabolic activity was found to be 11.5 μM for nanoparticles compared with 19.5 μM for free curcumin. PMID:25485110

  10. Inclusion bodies and purification of proteins in biologically active forms.

    PubMed

    Mukhopadhyay, A

    1997-01-01

    Even though recombinant DNA technology has made possible the production of valuable therapeutic proteins, its accumulation in the host cell as inclusion body poses serious problems in the recovery of functionally active proteins. In the last twenty years, alternative techniques have been evolved to purify biologically active proteins from inclusion bodies. Most of these remain only as inventions and very few are commercially exploited. This review summarizes the developments in isolation, refolding and purification of proteins from inclusion bodies that could be used for vaccine and non-vaccine applications. The second section involves a discussion on inclusion bodies, how they are formed, and their physicochemical properties. In vivo protein folding in Escherichia coli and kinetics of in vitro protein folding are the subjects of the third and fourth sections respectively. The next section covers the recovery of bioactive protein from inclusion bodies: it includes isolation of inclusion body from host cell debris, purification in denatured state alternate refolding techniques, and final purification of active molecules. Since purity and safety are two important issues in therapeutic grade proteins, the following three sections are devoted to immunological and biological characterization of biomolecules, nature, and type of impurities normally encountered, and their detection. Lastly, two case studies are discussed to demonstrate the sequence of process steps involved. PMID:8939059

  11. Optically active surfaces formed by ion implantation and thermal treatment

    SciTech Connect

    Gea, L.A.; Boatner, L.A.; Evans, H.M.; Zuhr, R.

    1996-08-01

    Embedded VO{sub 2} precipitates have been formed in single-crystal sapphire by the ion co-implantation of vanadium and oxygen and subsequent thermal annealing. The embedded VO{sub 2} particles have been shown to exhibit an optical switching behavior that is comparable to that of continuous thin films. In this work, the mechanisms of formation of these optically active particles are investigated. It is shown that precipitation of the vanadium dioxide phase is favored when the thermal treatment is performed on an ion-damaged but still crystalline (rather than amorphized) Al{sub 2}O{sub 3} substrate. The best optical switching behavior is observed in this case, and this behavior is apparently correlated with a more-favorable dispersion of VO{sub 2} small particles inside the matrix.

  12. 75 FR 9202 - Transparency Provisions of Section 23 of the Natural Gas Act; Notice of Form No. 552 Technical...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-01

    ... Energy Regulatory Commission Transparency Provisions of Section 23 of the Natural Gas Act; Notice of Form...\\ Transparency Provisions of Section 23 of the Natural Gas Act, Order No. 704, FERC Stats. & Regs. ] 31,260 (2007) (Final Rule); Transparency Provisions of Section 23 of the Natural Gas Act, Order No. 704-A, FERC...

  13. Chemical Fingerprints of Star Forming Regions and Active Galaxies

    NASA Astrophysics Data System (ADS)

    Pérez-Beaupuits, Juan-Pablo

    2010-10-01

    This thesis is devoted to the study of the physical conditions of the interstellar medium (ISM) in active galactic nuclei (AGNs) and Galactic star-forming regions, using mostly single-dish millimeter observations. I first study the excitation conditions of dense gas in a group of Seyfert galaxies using radiative transfer models (Chapter 2). I then study the galaxy NGC 1068, and try to distinguish signatures of the contributions from the AGN and the starburst ring by incorporating observations of high-J transitions of dense gas tracers (Chapter 3). Later, I venture into the mid-infrared spectral region to study different aspects of the AGN and starburst components in the galaxy NGC 4945 (Chapter 4). In Chapter 5 I delve into theoretical aspects of the dynamical evolution of gas in an AGN torus. I use a 3D hydrodynamic simulation with chemical abundances driven by X-rays. The aim is to understand the effects of X-ray irradiation by the AGN on the temperature, formation and destruction of the molecular gas. I finally explore a Galactic star-forming region, the Omega Nebula, with high resolution single dish observations, to study the properties of the warm gas and to constrain chemical models (Chapters 6 and 7).

  14. 75 FR 16492 - Agency Information Collection Activities: Form G-28, and Form G-28I, Revision of an Existing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-28, and Form G- 28I, Revision of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection under Review: Form G- 28, Notice of Entry of Appearance as Attorney or...

  15. Research report: Summary of key state issues of FERC orders 888 and 889

    SciTech Connect

    Rose, K.; Burns, R.E.; Graniere, R.J.

    1997-01-01

    The Federal Energy Regulatory Commission`s (FERC) Order 888 is perhaps the most important and far reaching FERC electricity order in decades. The consequences on the structure of the industry and how the industry is regulated are significant departures from past methods and regulatory philosophy. This will undoubtedly have a dramatic impact on the manner in which state public utility commissions, which are also undergoing or considering dramatic change, regulate their jurisdictional electric utilities. This report summarizes and discusses the actions that the FERC is taking and their profound repercussions on the industry and state commissions. The report is not a comprehensive summary of the entire order. Rather, it is intended to highlight the order`s more important features and discuss what this could mean for the states. The report is organized into eight sections; the first seven address Order 888 and the last section (section 8) addresses Order 889. Section 1 through 5 summarize and discuss the main features of Order 888. Section 6 (on jurisdiction) and Section 7 (on property rights) interpret the likely consequences of the order. Section 8, summarizes the FERC`s Open Access Same Time System (OASIS) and discusses some concerns about its real-world application.

  16. Conducting a FERC environmental assessment: a case study and recommendations from the Terror Lake Project

    SciTech Connect

    Olive, S.W.; Lamb, B.L.

    1983-09-01

    This paper is an account of the process that evolved during acquisition of the license to operate the Terror Lake hydro-electric power project under the auspices of the Federal Energy Regulatory Commission (FERC). The Terror River is located on Kodiak Island in Alaska. The river is within the Kodiak National Wildlife Refuge; it supports excellent runs of several species of Pacific Salmon which are both commercially important and a prime source of nutrition for the Kodiak brown bear. This paper discusses both the fish and wildlife questions, but concentrates on instream uses and how protection of these uses was decided. In this focus the paper explains the FERC process, gives a history of the Terror Lake Project, and, ultimately, makes recommendations for improved management of controversies within the context of FERC licensing procedures. 64 references.

  17. 75 FR 71123 - Notice of Commissioners and Staff Attendance at FERC/NARUC Collaborative on Smart Response Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Commissioners and Staff Attendance at FERC/NARUC Collaborative on... meeting: FERC/NARUC Collaborative on Smart Response: Omni Hotel at CNN Center, 100 CNN Center, Atlanta,...

  18. 76 FR 30738 - Agency Information Collection Activities: Form G-845 and Form G-845 Supplement, Revision of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-26

    ... the Federal Register on February 22, 2011, at 76 FR 9805, allowing for a 60-day public comment period... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-845 and Form G- 845 Supplement, Revision of a Currently Approved Information Collection; Comment Request...

  19. The Essay: Theory and Pedagogy for an Active Form.

    ERIC Educational Resources Information Center

    Heilker, Paul

    Calling for a radical reexamination of the traditional foundation of composition instruction--the thesis/support form, this book argues that the essay, with its informality, conversational tone, meditative mood, and integration of form and content, is better suited to developmental, epistemological, ideological, and feminist rhetorical…

  20. 77 FR 26537 - Notice of Commissioners and Staff Attendance at FERC Leadership Development Program Graduation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-04

    ... Energy Regulatory Commission Notice of Commissioners and Staff Attendance at FERC Leadership Development... Leadership Development Program Graduation/Induction Ceremony: 888 First Street NE., Washington, DC 20426. May... 2012 Leadership Development Program and graduate 15 employees from the 2011 program. Dated: April...

  1. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY... pipeline (to nearest 0.1 mile) between points of intake, delivery, river crossings, storage...

  2. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY... pipeline (to nearest 0.1 mile) between points of intake, delivery, river crossings, storage...

  3. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY... pipeline (to nearest 0.1 mile) between points of intake, delivery, river crossings, storage...

  4. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false System flow diagrams: Format No. FERC 567. 260.8 Section 260.8 Conservation of Power and Water Resources FEDERAL ENERGY... pipeline (to nearest 0.1 mile) between points of intake, delivery, river crossings, storage...

  5. 77 FR 50683 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ..., contact Peter Nagler, Office of Energy Market Regulation, Federal Energy Regulatory Commission at (202... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State...

  6. 77 FR 29634 - Notice of FERC Staff Attendance at the Louisiana Public Service Commission's Business and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... meeting is open to the public. For more information, contact Patrick Clarey, Office of Energy Market... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Louisiana Public Service...

  7. 76 FR 28024 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    ..., contact Patrick Clarey, Office of Energy Market Regulation, Federal Energy Regulatory Commission at (317... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State...

  8. 77 FR 33212 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-05

    ... Clarey, Office of Energy Market Regulation, Federal Energy Regulatory Commission at (317) 249- 5937 or... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State...

  9. 77 FR 63811 - Notice of Commissioners and Staff Attendance at FERC Author Speaker Series Event

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ... Energy Regulatory Commission Notice of Commissioners and Staff Attendance at FERC Author Speaker Series... the Commission and/or Commission staff may attend the following event: Author Speaker Series featuring... event will feature Pulitzer Prize winning author, Daniel Yergin, presenting on his most recent book,...

  10. 77 FR 20383 - Notice of Revisions to FERC Online, eSubscription Service

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-04

    ... Energy Regulatory Commission Notice of Revisions to FERC Online, eSubscription Service Take notice that on March 30, 2012, the Federal Energy Regulatory Commission (Commission) will update its eSubscription service that provides email notification when documents are added to eLibrary in a...

  11. 76 FR 76157 - California Independent System, Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-06

    ...; ER11-4580-000; ER12-50-000] California Independent System, Operator Corporation; Notice of FERC Staff... Quarterly Performance Review. December 1, 2011 Transmission Planning and Generator Interconnection. Working... Compliance. December 7, 2011 Market Performance and Planning Forum. Settlements and Market Clearing...

  12. 78 FR 34093 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission California Independent System Operator Corporation; Notice of FERC Staff Attendance The Federal Energy Regulatory Commission (Commission) hereby gives notice that on the following date members of its staff...

  13. 30 CFR 285.903 - What are the requirements for decommissioning FERC-licensed hydrokinetic facilities?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... FERC-licensed hydrokinetic facilities? 285.903 Section 285.903 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, REGULATION, AND ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE RENEWABLE ENERGY ALTERNATE USES... bond or other financial assurance; (b) You remain liable for removal or disposal costs and...

  14. Natural Gas Deliverability Task Force report: A joint FERC/DOE project. [Contains glossary

    SciTech Connect

    Not Available

    1992-09-01

    The purpose of the FERC/DOE Natural Gas Deliverability Task Force Report was threefold: (1) to review current deliverability data for utility, accuracy, and timeliness; (2) to identify mechanisms for closing significant gaps in information resulting from changing market structures; and (3) to ensure that technologies are available to meet the needs of the emerging, competitive natural gas industry.

  15. 77 FR 66609 - Notice of FERC Staff Attendance; PacifiCorp, et al.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance; PacifiCorp, et al. Docket No. Deseret Generation & Transmission ER13-65-000 Cooperative, Inc. Northwestern Corporation (Montana)..... ER13-67-000 Portland General Electric...

  16. Extensive sugar modification improves triple helix forming oligonucleotide activity in vitro but reduces activity in vivo.

    PubMed

    Alam, Md Rowshon; Majumdar, Alokes; Thazhathveetil, Arun Kalliat; Liu, Su-Ting; Liu, Ji-Lan; Puri, Nitin; Cuenoud, Bernard; Sasaki, Shigeki; Miller, Paul S; Seidman, Michael M

    2007-09-01

    We are developing triple helix forming oligonucleotides (TFOs) for gene targeting. Previously, we synthesized bioactive TFOs containing 2'-O-methylribose (2'-OMe) and 2'-O-aminoethylribose (2'-AE) residues. Active TFOs contained four contiguous 2'-AE residues and formed triplexes with high thermal stability and rapid association kinetics. In an effort to further improve bioactivity, we synthesized three series of TFOs containing the 2'-AE patch and additional ribose modifications distributed throughout the remainder of the oligonucleotide. These were either additional 2'-AE residues, the conformationally locked BNA/LNA ribose with a 2'-O,4'-C-methylene bridge, or the 2'-O,4'-C-ethylene analogue (ENA). The additionally modified TFOs formed triplexes with greater thermal stability than the reference TFO, and some had improved association kinetics. However, the most active TFOs in the biochemical and biophysical assays were the least active in the bioassay. We measured the thermal stability of triplexes formed by the TFOs in each series on duplex targets containing a change in sequence at a single position. The Tm value of the variant sequence triplexes increased as the number of all additional modifications increased. A simple explanation for the failure of the improved TFOs in the bioassay was that the increased affinity for nonspecific targets lowered the effective nuclear concentration. Enhancement of TFO bioactivity will require chemical modifications that improve interaction with the specific targets while retaining selectivity against mismatched sequences. PMID:17691818

  17. Conducting a FERC environmental assessment: a case study and recommendations from the Terror Lake Project

    SciTech Connect

    Olive, S.W.; Lamb, B.L.

    1984-04-01

    This paper is an account of the process that evolved during acquisition of the license to operate the Terror Lake hydroelectric power project under the auspices of the Federal Energy Regulatory Commission (FERC). The Commission is responsible for granting these licenses under the Federal Power Act (16 U.S.C. 792 et seq.). This act provides, in part, that FERC may condition a license to protect the public interest. The public interest in these cases has come to include both instream and terrestrial values. The Terror River is located on Kodiak Island in Alaska. The river is within the Kodiak National Wildlife Refuge; it supports excellent runs of several species of Pacific Salmon which are both commercially important and a prime source of nutrition for the Kodiak brown bear. The river is also a prime resource for generating electric power. One major concern in the negotiations was the impact of land disturbance and management practices on brown bear habitat - i.e., protection of the brown bear. Maintenance of the bears' habitat is the main purpose of the Kodiak National Wildlife Refuge. But, like many other projects, resolving the instream flow issue was of major importance in the issuance of the FERC license. This paper discusses the fish and wildlife questions, but concentrates on instream uses and how protection of these uses was decided. With this as a focus, the paper explains the FERC process, gives a history of the Terror Lake Project, and, ultimately, makes recommendations for improved management of controversies within the context of the FERC licensing procedures. 65 references.

  18. STYPu fuel form activities, March 1-September 30, 1985

    SciTech Connect

    Not Available

    1986-01-01

    The SRP portion of this report summarizes production STYPuO2 fuel forms for use in radioisotopic thermoelectric generators (RTG's) in the Plutonium Fuel Form (PuFF) Facility at the Savannah River Plant. The PuFF Facility began producing iridium-encapsulated, 62.5-watt STYPuO2 right circular cylinders for GPHS (General Purpose Heat Source) RTG's in June 1980; this program was completed in December 1983. The PuFF Facility has been placed in a production readiness mode of operation pending funding of additional heat source programs.

  19. Mechanically driven activation of polyaniline into its conductive form.

    PubMed

    Baytekin, Bilge; Baytekin, H Tarik; Grzybowski, Bartosz A

    2014-07-01

    Mechanical treatment of polymers produces surface cations and anions which, as demonstrated here for the first time, can drive chemical reactions. In particular, it is shown that such a mechanical treatment transforms nonconductive polyaniline into its conductive form. These results provide a mechanical means of patterning conductive polymers and also coating small polymer objects with conductive polyaniline films preventing accumulation of static electricity. PMID:24824971

  20. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable. SPECIFIC... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from...

  1. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Pipeline Data for Depreciation Analysis. 357.3 Section 357.3 Conservation of Power and Water Resources... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... 73, Oil Pipeline Data for Depreciation Analysis, available for review at the Commission's...

  2. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from a... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable....

  3. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from a... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable....

  4. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable. SPECIFIC... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from...

  5. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to... access to, CEII, shall apply in the event of a challenge to a CEII designation or a request for access to... until approved by the Office of Management and Budget....

  6. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... report is mandatory and is prescribed by Section 305(c)(1) of the Federal Power Act and 18 CFR 46.4... requirements prescribed in 18 CFR 46.4. 2. Leave blank any columns that are not applicable. SPECIFIC... utility 20CL Entity specified in 18 CFR 46.3 (one of the 20 largest purchasers of electric energy from...

  7. Thrombomodulin Binding Selects the Catalytically Active Form of Thrombin.

    PubMed

    Handley, Lindsey D; Treuheit, Nicholas A; Venkatesh, Varun J; Komives, Elizabeth A

    2015-11-01

    Human α-thrombin is a serine protease with dual functions. Thrombin acts as a procoagulant, cleaving fibrinogen to make the fibrin clot, but when bound to thrombomodulin (TM), it acts as an anticoagulant, cleaving protein C. A minimal TM fragment consisting of the fourth, fifth, and most of the sixth EGF-like domain (TM456m) that has been prepared has much improved solubility, thrombin binding capacity, and anticoagulant activity versus those of previous TM456 constructs. In this work, we compare backbone amide exchange of human α-thrombin in three states: apo, D-Phe-Pro-Arg-chloromethylketone (PPACK)-bound, and TM456m-bound. Beyond causing a decreased level of amide exchange at their binding sites, TM and PPACK both cause a decreased level of amide exchange in other regions including the γ-loop and the adjacent N-terminus of the heavy chain. The decreased level of amide exchange in the N-terminus of the heavy chain is consistent with the historic model of activation of serine proteases, which involves insertion of this region into the β-barrel promoting the correct conformation of the catalytic residues. Contrary to crystal structures of thrombin, hydrogen-deuterium exchange mass spectrometry results suggest that the conformation of apo-thrombin does not yet have the N-terminus of the heavy chain properly inserted for optimal catalytic activity, and that binding of TM allosterically promotes the catalytically active conformation. PMID:26468766

  8. Forming a Learning Culture to Promote Fracture Prevention Activities

    ERIC Educational Resources Information Center

    Hjalmarson, Helene V.; Strandmark, Margaretha

    2012-01-01

    Purpose: The purpose of this paper is to explore interprofessional experiences of incorporating fracture prevention activities in clinical practice inspired by an empowerment approach. Design/methodology/approach: Data collection consisted primarily of focus groups interviews, systematized and analyzed by the grounded theory method. The study took…

  9. How Lipid Membranes Affect Pore Forming Toxin Activity.

    PubMed

    Rojko, Nejc; Anderluh, Gregor

    2015-12-15

    Pore forming toxins (PFTs) evolved to permeate the plasma membrane of target cells. This is achieved in a multistep mechanism that usually involves binding of soluble protein monomer to the lipid membrane, oligomerization at the plane of the membrane, and insertion of part of the polypeptide chain across the lipid membrane to form a conductive channel. Introduced pores allow uncontrolled transport of solutes across the membrane, inflicting damage to the target cell. PFTs are usually studied from the perspective of structure-function relationships, often neglecting the important role of the bulk membrane properties on the PFT mechanism of action. In this Account, we discuss how membrane lateral heterogeneity, thickness, and fluidity influence the pore forming process of PFTs. In general, lipid molecules are more accessible for binding in fluid membranes due to steric reasons. When PFT specifically binds ordered domains, it usually recognizes a specific lipid distribution pattern, like sphingomyelin (SM) clusters or SM/cholesterol complexes, and not individual lipid species. Lipid domains were also suggested to act as an additional concentration platform facilitating PFT oligomerization, but this is yet to be shown. The last stage in PFT action is the insertion of the transmembrane segment across the membranes to build the transmembrane pore walls. Conformational changes are a spontaneous process, and sufficient free energy has to be available for efficient membrane penetration. Therefore, fluid bilayers are permeabilized more readily in comparison to highly ordered and thicker liquid ordered lipid phase (Lo). Energetically more costly insertion into the Lo phase can be driven by the hydrophobic mismatch between the thinner liquid disordered phase (Ld) and large protein complexes, which are unable to tilt like single transmembrane segments. In the case of proteolipid pores, membrane properties can directly modulate pore size, stability, and even selectivity. Finally

  10. Active Curved Polymers Form Vortex Patterns on Membranes

    NASA Astrophysics Data System (ADS)

    Denk, Jonas; Huber, Lorenz; Reithmann, Emanuel; Frey, Erwin

    2016-04-01

    Recent in vitro experiments with FtsZ polymers show self-organization into different dynamic patterns, including structures reminiscent of the bacterial Z ring. We model FtsZ polymers as active particles moving along chiral, circular paths by Brownian dynamics simulations and a Boltzmann approach. Our two conceptually different methods point to a generic phase behavior. At intermediate particle densities, we find self-organization into vortex structures including closed rings. Moreover, we show that the dynamics at the onset of pattern formation is described by a generalized complex Ginzburg-Landau equation.

  11. Active Curved Polymers Form Vortex Patterns on Membranes.

    PubMed

    Denk, Jonas; Huber, Lorenz; Reithmann, Emanuel; Frey, Erwin

    2016-04-29

    Recent in vitro experiments with FtsZ polymers show self-organization into different dynamic patterns, including structures reminiscent of the bacterial Z ring. We model FtsZ polymers as active particles moving along chiral, circular paths by Brownian dynamics simulations and a Boltzmann approach. Our two conceptually different methods point to a generic phase behavior. At intermediate particle densities, we find self-organization into vortex structures including closed rings. Moreover, we show that the dynamics at the onset of pattern formation is described by a generalized complex Ginzburg-Landau equation. PMID:27176542

  12. 75 FR 21013 - Agency Information Collection Activities: Form N-644; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-22

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-644... Collection Under Review; Form N- 644, Application for Posthumous Citizenship; OMB Control No. 1615-0059. The..., USCIS will be evaluating whether to revise the Form N-644. Should USCIS decide to revise Form N-644...

  13. 76 FR 21913 - Agency Information Collection Activities: Form N-644; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-19

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-644... Collection Under Review: Form N- 644, Application for Posthumous Citizenship; OMB Control No. 1615-0059. The..., USCIS will be evaluating whether to revise the Form N-644. Should USCIS decide to revise Form N-644...

  14. 75 FR 51095 - Agency Information Collection Activities: Form N-336; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-336... Collection under Review; Form N- 336, Request for Hearing on a Decision in Naturalization Proceedings Under... the Form N-336. Should USCIS decide to revise Form N-336 we will advise the public when we publish...

  15. Heat of Hydration of Low Activity Cementitious Waste Forms

    SciTech Connect

    Nasol, D.

    2015-07-23

    During the curing of secondary waste grout, the hydraulic materials in the dry mix react exothermally with the water in the secondary low-activity waste (LAW). The heat released, called the heat of hydration, can be measured using a TAM Air Isothermal Calorimeter. By holding temperature constant in the instrument, the heat of hydration during the curing process can be determined. This will provide information that can be used in the design of a waste solidification facility. At the Savannah River National Laboratory (SRNL), the heat of hydration and other physical properties are being collected on grout prepared using three simulants of liquid secondary waste generated at the Hanford Site. From this study it was found that both the simulant and dry mix each had an effect on the heat of hydration. It was also concluded that the higher the cement content in the dry materials mix, the greater the heat of hydration during the curing of grout.

  16. Identification of biotransformation products of citalopram formed in activated sludge.

    PubMed

    Beretsou, Vasiliki G; Psoma, Aikaterini K; Gago-Ferrero, Pablo; Aalizadeh, Reza; Fenner, Kathrin; Thomaidis, Nikolaos S

    2016-10-15

    Citalopram (CTR) is a worldwide highly consumed antidepressant which has demonstrated incomplete removal by conventional wastewater treatment. Despite its global ubiquitous presence in different environmental compartments, little is known about its behaviour and transformation processes during wastewater treatment. The present study aims to expand the knowledge on fate and transformation of CTR during the biological treatment process. For this purpose, batch reactors were set up to assess biotic, abiotic and sorption losses of this compound. One of the main objectives of the study was the identification of the formed transformation products (TPs) by applying suspect and non-target strategies based on liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS). The complementary use of reversed phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) for the identification of polar TPs, and the application of in-house developed quantitative structure-retention relationship (QSRR) prediction models, in addition to the comprehensive evaluation of the obtained MS/MS spectra, provided valuable information to support identification. In total, fourteen TPs were detected and thirteen of them were tentatively identified. Four compounds were confirmed (N-desmethylCTR, CTR amide, CTR carboxylic acid and 3-oxo-CTR) through the purchase of the corresponding reference standard. Probable structures based on diagnostic evidence were proposed for the additional nine TPs. Eleven TPs are reported for the first time. A transformation pathway for the biotransformation of CTR was proposed. The presence of the identified TPs was assessed in real wastewater samples through retrospective analysis, resulting in the detection of five compounds. Finally, the potential ecotoxicological risk posed by CTR and its TPs to different trophic levels of aquatic organisms was evaluated by means of risk quotients. PMID:27459150

  17. A conflict in the circuits: The FERC's jurisdication over gathering rates

    SciTech Connect

    Not Available

    1992-01-01

    There is a ripe debate about the extent of the Federal Energy Regulatory Commission's (FERC or Commission) jurisdiction over certain gas gathering rates. This issue will be examined by considering two recent cases and their differing analyses of section 1(b) of the Natural Gas Act (NGA), which defines the scope of authority granted by Congress to the Federal Power Commission. Section 1(b) of the NGA states: The provisions of this chapter shall apply to the transportation of natural gas in interstate commerce, to the sale in interstate commerce of natural gas for resale for ultimate public consumption for domestic, commercial, industrial, or any other use, and to natural-gas companies engaged in such transportation or sale, but shall not apply to any other transportation or sale of natural gas or to the local distribution of natural gas or to the facilities used for such distribution 2 or to the production or gathering of natural gas (emphasis added). Gathering is the act of transporting gas as it is collected from the wells, whether or not the movement is interstate. Gathering systems consist of pipeline and pumping units used to bring oil or gas from production leases by separate lines to a central point. Gathering can be performed by a producer, by a pipeline, or by an independent gathering company. The cases of Northwest Pipeline Corp. vs. FERC' and Northern Natural Gas Co. vs. FERC' were found to have the same common thread in the beginning of each suit, a challenge that a gathering charge was excessive. Each Circuit went in different directions in assessing the FERC's jurisdiction.

  18. Wound healing activity of topical application forms based on ayurveda.

    PubMed

    Datta, Hema Sharma; Mitra, Shankar Kumar; Patwardhan, Bhushan

    2011-01-01

    The traditional Indian medicine-Ayurveda, describes various herbs, fats, oils and minerals with anti-aging as well as wound healing properties. With aging, numerous changes occur in skin, including decrease in tissue cell regeneration, decrease in collagen content, loss of skin elasticity and mechanical strength. We prepared five topical anti-aging formulations using cow ghee, flax seed oil, Phyllanthus emblica fruits, Shorea robusta resin, Yashada bhasma as study materials. For preliminary efficacy evaluation of the anti-aging activity we chose excision and incision wound healing animal models and studied the parameters including wound contraction, collagen content and skin breaking strength which in turn is indicative of the tissue cell regeneration capacity, collagenation capacity and mechanical strength of skin. The group treated with the formulations containing Yashada bhasma along with Shorea robusta resin and flax seed oil showed significantly better wound contraction (P < .01), higher collagen content (P < .05) and better skin breaking strength (P < .01) as compared to control group; thus proposing them to be effective prospective anti-aging formulations. PMID:19252191

  19. Wound Healing Activity of Topical Application Forms Based on Ayurveda

    PubMed Central

    Datta, Hema Sharma; Mitra, Shankar Kumar; Patwardhan, Bhushan

    2011-01-01

    The traditional Indian medicine—Ayurveda, describes various herbs, fats, oils and minerals with anti-aging as well as wound healing properties. With aging, numerous changes occur in skin, including decrease in tissue cell regeneration, decrease in collagen content, loss of skin elasticity and mechanical strength. We prepared five topical anti-aging formulations using cow ghee, flax seed oil, Phyllanthus emblica fruits, Shorea robusta resin, Yashada bhasma as study materials. For preliminary efficacy evaluation of the anti-aging activity we chose excision and incision wound healing animal models and studied the parameters including wound contraction, collagen content and skin breaking strength which in turn is indicative of the tissue cell regeneration capacity, collagenation capacity and mechanical strength of skin. The group treated with the formulations containing Yashada bhasma along with Shorea robusta resin and flax seed oil showed significantly better wound contraction (P < .01), higher collagen content (P < .05) and better skin breaking strength (P < .01) as compared to control group; thus proposing them to be effective prospective anti-aging formulations. PMID:19252191

  20. [L forms of Staphylococcus aureus. Behavior of coagulase, hemolytic and desoxyribonuclease activities and antibiotic sensitivity].

    PubMed

    Loschiavo, F; Giarrizzo, S

    1977-01-01

    L Forms derived from strains of coagulase positive Staphylococcus aureus, have, on the whole, preserved their DNAsic, haemolitic and coagulastic activities. L. forms showed high resistence to antibiotics acting on the bacterial cell-wall. The sensibility to other antibiotics was, roughly, analogous for the L forms as well as for the bacterial strains ones, with the exception of the clortetraciclin and the diidrostreptomicin, ehich proved to be comparatively more active on the L forms. PMID:614141

  1. Composition and topology of activity cliff clusters formed by bioactive compounds.

    PubMed

    Stumpfe, Dagmar; Dimova, Dilyana; Bajorath, Jürgen

    2014-02-24

    The assessment of activity cliffs has thus far mostly focused on compound pairs, although the majority of activity cliffs are not formed in isolation but in a coordinated manner involving multiple active compounds and cliffs. However, the composition of coordinated activity cliff configurations and their topologies are unknown. Therefore, we have identified all activity cliff configurations formed by currently available bioactive compounds and analyzed them in network representations where activity cliff configurations occur as clusters. The composition, topology, frequency of occurrence, and target distribution of activity cliff clusters have been determined. A limited number of large cliff clusters with unique topologies were identified that were centers of activity cliff formation. These clusters originated from a small number of target sets. However, most clusters were of small to moderate size. Three basic topologies were sufficient to describe recurrent activity cliff cluster motifs/topologies. For example, frequently occurring clusters with star topology determined the scale-free character of the global activity cliff network and represented a characteristic activity cliff configuration. Large clusters with complex topology were often found to contain different combinations of basic topologies. Our study provides a first view of activity cliff configurations formed by currently available bioactive compounds and of the recurrent topologies of activity cliff clusters. Activity cliff clusters of defined topology can be selected, and from compounds forming the clusters, SAR information can be obtained. The SAR information of activity cliff clusters sharing a/one specific activity and topology can be compared. PMID:24437577

  2. 76 FR 69276 - Agency Information Collection Activities: Form N-336, Revision of a Currently Approved...

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    ... SECURITY Citizenship and Immigration Services Agency Information Collection Activities: Form N-336... Information Collection Under Review: Form N- 336, Request for Hearing on a Decision in Naturalization...: Form N-336. U.S. Citizenship and Immigration Services. (4) Affected public who will be asked...

  3. 76 FR 53144 - Agency Information Collection Activities: Form N-336; Revision of a Currently Approved...

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    2011-08-25

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-336... Information Collection Under Review: Form N- 336, Request for Hearing on a Decision in Naturalization...: Form N-336; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  4. 75 FR 78264 - Agency Information Collection Activities: Form N-336, Revision to an Existing Information...

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    2010-12-15

    ... Register at 75 FR 51095 announcing the extension of the Form N-336. The 60-day notice announced that during... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-336... collection under review: Form N- 336, Request for Hearing on a Decision in Naturalization Proceedings...

  5. 76 FR 78674 - Agency Information Collection Activities: Form N-470, Revision of a Currently Approved...

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    2011-12-19

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  6. 75 FR 51096 - Agency Information Collection Activities: Form N-400; Extension of an Existing Information...

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    2010-08-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-400... Collection Under Review; Form N- 400, Application for Naturalization; OMB Control No. 1615-0052. The... 60 day period, USCIS will be evaluating whether to revise the Form N-400. Should USCIS decide...

  7. 75 FR 13776 - Agency Information Collection Activities: Form N-300; Extension of an Existing Information...

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    2010-03-23

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  9. 75 FR 70278 - Agency Information Collection Activities: Form N-600, Extension of a Currently Approved...

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  10. 76 FR 69275 - Agency Information Collection Activities: Form N-400, Extension of a Currently Approved...

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    2011-11-08

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  11. 75 FR 70277 - Agency Information Collection Activities: Form N-400, Extension of a Currently Approved...

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    2010-11-17

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  12. 76 FR 59710 - Agency Information Collection Activities: Form N-600; Revision of an Existing Information...

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  13. 76 FR 27078 - Agency Information Collection Activities: Form N-426, Extension of a Currently Approved...

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    2011-05-10

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  14. 77 FR 24507 - Agency Information Collection Activities: Form N-25, Extension of an Existing Information...

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  15. 75 FR 43535 - Agency Information Collection Activities: Form N-644, Revision of a Currently Approved...

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    2010-07-26

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  16. 75 FR 5098 - Agency Information Collection Activities: Form N-565, Extension of a Currently Approved...

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    2010-02-01

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-565... information collection under review: Form N- 565, Application for Replacement Naturalization/Citizenship... Homeland Security sponsoring the collection: Form N-565; U.S. Citizenship and Immigration Services...

  17. 75 FR 80835 - Agency Information Collection Activities: Form N-565; Extension of an Existing Information...

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    2010-12-23

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-565... Collection Under Review; Form N- 565, Application for Replacement Naturalization/Citizenship Document; OMB... N-565. Should USCIS decide to revise Form N-565 we will advise the public when we publish the...

  18. 76 FR 39415 - Agency Information Collection Activities: Form N-644, Extension of a Currently Approved...

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    2011-07-06

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  19. 75 FR 30050 - Agency Information Collection Activities: Form N-648, Revision of an Existing Information...

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  20. 77 FR 18255 - Agency Information Collection Activities: Form N-565; Extension of an Existing Information...

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    2012-03-27

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-565... collection under review; Form N- 565, Application for Replacement Naturalization/Citizenship Document; OMB... until May 29, 2012. During this 60 day period, USCIS will be evaluating whether to revise the Form...

  1. 75 FR 70277 - Agency Information Collection Activities: Form N-336, Extension of a Currently Approved...

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    2010-11-17

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-336... Information Collection Under Review: Form N- 336, Request for Hearing on a Decision in Naturalization... collection: Form N-336; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will...

  2. 75 FR 51096 - Agency Information Collection Activities: Form N-470; Extension of an Existing Information...

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    2010-08-18

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-470... Collection Under Review; Form N- 470, Application To Preserve Residence for Naturalization; OMB Control No... until October 18, 2010. During this 60-day period, USCIS will be evaluating whether to revise the Form...

  3. 77 FR 128 - Agency Information Collection Activities: Form N-600, Revision of a Currently Approved...

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  4. 76 FR 28444 - Agency Information Collection Activities: Form G-884, Extension of a Currently Approved...

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    2011-10-12

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  7. 76 FR 24908 - Agency Information Collection Activities: Form G-639; Extension of an Existing Information...

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    2011-05-03

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-639... Collection Under Review; Form G- 639, Freedom of Information/Privacy Act Request; OMB Control No. 1615- 0102.... During this 60 day period, USCIS will be evaluating whether to revise the Form G-639. Should USCIS...

  8. 78 FR 67397 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Short-Form...

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  9. 78 FR 27965 - Agency Information Collection Activities: Submission for OMB Review; Comment Request Re Forms...

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    2013-05-13

    ... by the PRA. On February 7, 2013 (78 FR 9049), the FDIC solicited public comment for a 60-day period.../04 0.50 30 15 Declaration for Revocable Trust, Form 7200/05 0.50 150 75 Declaration of Independent Activity, Form 7200/06 0.50 5 2.5 Declaration of Independent Activity for Unincorporated 0.50 5...

  10. Dance as a Fitness Activity: The Impact of Teaching Style and Dance Form.

    ERIC Educational Resources Information Center

    Fromel, Karel; Vasendova, Jana; Stratton, Gareth; Pangrazi, Robert P.

    2002-01-01

    Analyzed the amount of activity, intensity of activity, and attitudes of participants in Czech high school physical education classes taught using different teaching styles and dance forms. Measurements of heart rate and dance intensity and student surveys indicated that teaching style and dance form significantly impacted the intensity and volume…

  11. In vitro activity of Rutaceae species against the trypomastigote form of Trypanosoma cruzi.

    PubMed

    Mafezoli, J; Vieira, P C; Fernandes, J B; da Silva, M F; de Albuquerque, S

    2000-11-01

    The activity of crude plant extracts of nine species of Rutaceae against the trypomastigote form of Trypanosoma cruzi was evaluated at 4 mg/ml. Thirty-two crude extracts were tested and eight of them showed significant activity (>80%). The most active extract was obtained from the stems of Pilocarpus spicatus (97.3%). Fractionation of the active crude extracts provided 25 fractions which were tested against the trypomastigote form of T. cruzi at 2 mg/ml. Of these six showed significant activity (>80%). The most active fractions (100%) were obtained from the leaves of Almeidea coerulea (butanol fraction) and Conchocarpus inopinatus (dichloromethane fraction). PMID:11025175

  12. Assessing the "need to negotiate" in FERC licensing consultations: a study of two hydropower projects

    USGS Publications Warehouse

    Coughlan, Beth A. K.; Burkardt, Nina; Fulton, David

    1993-01-01

    We investigated the “need to negotiate” in a comparative case study of multi-agency negotiations in the FERC licensing process. Researchers interviewed participants in two cases involving environmental consultations and asked about parties' level of need to negotiate throughout the process. Participants identified a need to negotiate, and when this need was strongly felt, there was an increased opportunity for an agreement to be reached. An intense need to negotiate by all parties is not a prerequisite to successful agreements. When key participants have a strong need to negotiate, they can instigate negotiations and encourage the involvement of other parties.

  13. Fifth circuit expands not substantially developed exception to FERC jurisdiction under the Natural Gas Act of 1938

    SciTech Connect

    Eppler, D.

    1985-07-01

    The El Paso Natural Gas Co. v. Sun Oil Co. suit found that the Federal Energy Regulatory Commission (FERC) does not have jurisdiction over the sale of leases covering proven reserves of natural gas that lack the imminent ability to produce in commercial quantities. A review of the case and an analysis of the Court's decision concludes that it seems more logical to define the limits of the production and gathering exception in terms of commerce clause and federalism rather than in terms of the number of wells drilled at the time of sale. FERC's jurisdiction under the Natural Gas Act ought to be defined to reach wherever the state's regulatory jurisdiction cannot. Such an interpretation of FERC jurisdiction would further the purpose of the Natural Gas Act, which is to protect consumers from exploitation by natural gas suppliers by regulating the price at which large quantities of gas are transported and sold in interstate commerce.

  14. From gas to electric at FERC: Will it be deja vu all over again. [Federal Energy Regulatory Commission

    SciTech Connect

    Bobbish, D.J. )

    1992-06-01

    Recent legislative proposals to increase FERC's authority to require electric utilities to provide transmission service to third parties ( wheeling') and to encourage the development of independent power producers (IPPs) suggest that FERC may soon have the necessary legislative mandate to 436' the electric utility industry - to pursue at a faster pace the same sort of restructuring it has been following since at least 1988. In view of the possibility that the electric industry may be reformed in a way that parallels the natural gas industry in some fashion, this paper examines how the Commission has gone about restructuring the natural gas industry over the past seven years, and what lessons the Commission should have learned from that experience and whether recent developments at FERC on the electric side indicate that it has learned those lessons.

  15. 75 FR 30098 - Reports, Forms and RecordKeeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-28

    ... to help States enhance motorcyclist safety training and motorcyclist awareness programs. To qualify... National Highway Traffic Safety Administration Reports, Forms and RecordKeeping Requirements; Agency Information Collection Activity Under OMB Review AGENCY: National Highway Traffic Safety Administration,...

  16. 76 FR 52014 - Agency Information Collection Activities: Applicant Information Form (1-783)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF JUSTICE Federal Bureau of Investigation Agency Information Collection Activities: Applicant Information Form (1-783) ACTION: 30-Day Notice of Information Collection. The Department of Justice (DOJ), Federal...

  17. 76 FR 70747 - Agency Information Collection Activities: Form I-90; Revision of a Currently Approved Information...

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    2011-11-15

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-90... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the...: Form I-90; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  18. 76 FR 9805 - Agency Information Collection Activities: Form G-845 and Supplement; Revision of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-22

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-845 and.... Citizenship and Immigration Services (USCIS) will be submitting the following information collection request... collection: Form G-845 and Supplement. U.S. Citizenship and Immigration Services. (4) Affected public...

  19. 77 FR 16047 - Agency Information Collection Activities: Form I-589; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-19

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-589.... 1615-0067. The Department Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be...: Form I-589; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  20. 76 FR 52961 - Agency Information Collection Activities: Form N-300; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-24

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-300.... The Department Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting... collection: Form N-300; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will...

  1. 77 FR 27473 - Agency Information Collection Activities: Form I-924; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-924... Department of Homeland Security (DHS), U.S. Citizenship and Immigration Services (USCIS) will be submitted... sponsoring the collection: Form I-924; U.S. Citizenship and Immigration Services. (4) Affected public...

  2. 77 FR 21104 - Agency Information Collection Activities: Form I-694, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-09

    ... a brief abstract: Primary: Individuals and households. USCIS uses the information provided on Form I... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-694, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice...

  3. 76 FR 20361 - Agency Information Collection Activities: Form I-694, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-694, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 694, Notice of Appeal of Decision Under Section 210 or 245A;...

  4. 75 FR 74071 - Agency Information Collection Activities: Form I-601, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-30

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-601, Revision of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 601, Application for Waiver of Grounds of Inadmissibility;...

  5. 76 FR 20362 - Agency Information Collection Activities: Form I-905, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-905, Extension of a Currently Approved Information Collection; Comment Request Action: 60-Day Notice of Information Collection Under Review: Form I- 905, Application for Authorization To Issue Certification...

  6. 76 FR 20361 - Agency Information Collection Activities: Form I-907, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-907, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-day notice of information collection under review: Form I- 907, Request for Premium Processing Service; OMB Control No....

  7. 75 FR 52539 - Agency Information Collection Activities: Form I-777, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-26

    ... information collection was previously published in the Federal Register on June 9, 2010, at 75 FR 32799... asked or required to respond, as well as a brief abstract: Primary: Individuals or Households. Form I... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form...

  8. 75 FR 11898 - Agency Information Collection Activities: Form I-612, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-12

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-612, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 612, Application for Waiver of the Foreign Residence...

  9. 76 FR 66944 - Agency Information Collection Activities: Form I-129F; Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-28

    ... SECURITY Citizenship and Immigration Services Agency Information Collection Activities: Form I-129F.... The Department of Homeland Security, U.S. Citizenship and Immigration Services will be submitting the... Department of Homeland Security sponsoring the collection: Form I-129F, U.S. Citizenship and...

  10. 76 FR 81517 - Agency Information Collection Activities: Form I-131, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-131, Revision of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form I- 131,...

  11. 75 FR 18871 - Agency Information Collection Activities: Form N-600K, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-600K, Revision of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection under Review: Form N-...

  12. 75 FR 41216 - Agency Information Collection Activities: Form N-644, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-644... Information Collection Under Review: Form N- 644, Application for Posthumous Citizenship; OMB Control No. 1615... N-644; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  13. 75 FR 14179 - Agency Information Collection Activities: Form I-9 CNMI; Revision to an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-24

    ... previously published in the Federal Register on December 31, 2009, at 74 FR 69354, allowing for a 60-day... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-9 CNMI... Collection under Review: Form I-9 CNMI, CNMI Employment Eligibility Verification; OMB Control No. 1615-...

  14. 75 FR 32801 - Agency Information Collection Activities: Form I-865; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-09

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-865... Collection Under Review: Form I- 865, Sponsor's Notice of Change of Address; OMB Control Number 1615- 0076. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) has...

  15. 76 FR 53929 - Agency Information Collection Activities: Form G-639, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ... published in the Federal Register on May 3, 2011, at 76 FR 24908, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-639... Information Collection Under Review: Form G- 639, Freedom of Information/Privacy Act Request. * * * * *...

  16. 77 FR 21105 - Agency Information Collection Activities: Form G-1145, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-09

    ..., at 77 FR 3278, allowing for a 60-day public comment period. USCIS did not receive any comments for... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-1145... information collection under review: Form G- 1145, E-Notification of Application/Petition Acceptance;...

  17. 76 FR 11807 - Agency Information Collection Activities: Form G-646, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... December 15, 2010, at 75 FR 78263, allowing for a 60-day public comment period. USCIS did not receive any... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-646... Information Collection Under Review: Form G- 646, Sworn Statement of Refugee Applying for Admission to...

  18. 77 FR 12071 - Agency Information Collection Activities: Form G-28, Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... notice was previously published in the Federal Register on October 12, 2011, at 76 FR 63322, allowing for... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-28... Information Collection Under Review: Form G- 28, Notice of Entry of Appearance as Attorney or...

  19. 76 FR 11806 - Agency Information Collection Activities: Form G-1145, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... information collection was previously published in the Federal Register on December 14, 2010, at 75 FR 77890... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-1145... Information Collection Under Review: Form G- 1145, E-Notification of Application/Petition Acceptance;...

  20. 76 FR 48874 - Agency Information Collection Activities: Form G-884, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... the Federal Register on March 17, 2011, at 76 FR 28444, allowing for a 60- day public comment period... SECURITY Citizenship and Immigration Services Agency Information Collection Activities: Form G-884... Collection Under Review: Form G- 884, Request for the Return of Original Document(s). The Department...

  1. 75 FR 5097 - Agency Information Collection Activities: Form G-646, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... 74 FR 58037, allowing for a 60-day public comment period. USCIS did not receive any comments for this... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-646... Information Collection Under Review: Form G- 646, Sworn Statement of Refugee Applying for Admission to...

  2. 77 FR 33760 - Agency Information Collection Activities: Form G-646, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... collection was previously published in the Federal Register on March 9, 2012, at 77 FR 14407, allowing for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-646... Information Collection Under Review: Form G- 646, Sworn Statement of Refugee Applying for Admission to...

  3. 75 FR 47822 - Agency Information Collection Activities: Form G-639, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... collection was previously published in the Federal Register on May 4, 2010, at 75 FR 23785, allowing for a 60... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form G-639... Information Collection Under Review: Form G- 639, Freedom of Information/Privacy Act Request; OMB Control...

  4. 75 FR 57049 - Agency Information Collection Activities: Form I-777, Application for Replacement of Northern...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-777, Application for Replacement of Northern Mariana Card ACTION: Correction to 30-day notice of Information Collection Under Review: Form...

  5. 75 FR 10298 - Agency Information Collection Activities: Form I-929; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-05

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-929; Extension of an Existing Information Collection; Comment Request ACTION: 30 Day Notice of Information Collection Under Review; Form I-929,...

  6. 76 FR 10387 - Agency Information Collection Activities: Form I-929; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-929; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form I- 929,...

  7. 78 FR 52824 - Proposed Information Collection (Bowel and Bladder Care Billing Form) Activity: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-26

    ... AFFAIRS Proposed Information Collection (Bowel and Bladder Care Billing Form) Activity: Comment Request... evaluate the Bowel and Bladder Care Billing Form used by caregivers of eligible Veterans to document time spent providing services related specifically to bowel and bladder care. DATES: Written comments...

  8. 76 FR 41282 - Agency Information Collection Activities: Form I-363, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Citizenship and Immigration Services Agency Information Collection Activities: Form I-363, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form I-...

  9. 76 FR 21912 - Agency Information Collection Activities: Form I-363; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-19

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-363... Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the following information... the Department of Homeland Security sponsoring the collection: Form I-363, U.S. Citizenship...

  10. 75 FR 35822 - Agency Information Collection Activities: Form I-363; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-363... Control No. 1615-0022. The Department of Homeland Security, U.S. Citizenship and Immigration Services... Homeland Security sponsoring the collection: Form I-363; U.S. Citizenship and Immigration Services...

  11. 78 FR 73875 - Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-09

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68) AGENCY: U.S. Customs and Border Protection (CBP), Department of Homeland... requirement concerning the Canadian Border Boat Landing Permit (Form I-68). This request for comment is...

  12. 75 FR 61508 - Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68) AGENCY: U.S. Customs and Border Protection (CBP), Department of Homeland... concerning the Canadian Border Boat Landing Permit (Form I- ] 68). This request for comment is being...

  13. Effect of chemical form of selenium on tissue glutathione peroxidase activity in developing rats

    NASA Technical Reports Server (NTRS)

    Lane, Helen W.; Strength, Ralph; Johnson, Janet; White, Marguerite T.

    1991-01-01

    The hypothesis that the stage of development of rats may affect the availability of various forms of selenium for the activity of glutathione peroxidase (GSHPx) in the rat was experimentally investigated. One experiment evaluated the availability of selenium as selenite or selenomethionine for GSPHx activity during three developmental states in rats: fetus and 7-day old and 14-day old nursing pups. In all tissues studied, GSHPx activity was highest in the 14-day-old pups whose dams were in the selenomethionine group. Rat pups given intraperitoneal selenite had higher liver and kidney GSHPx activity than pups given the same amount of selenium as intraperitoneal selenomethionine. In a second experiment, all dams were fed the same basal diet and pups were weaned to diets containing one of two levels of selenium and one of three forms of selenium (selenite, selenomethionine, or selenocystine). The results also supported the hypothesis these dietary forms of selenium are differentially available for GSHPx activity.

  14. Differential Expression of Extracellular Lipase and Protease Activities of Mycelial and Yeast Forms in Malassezia furfur.

    PubMed

    Juntachai, Weerapong; Kajiwara, Susumu

    2015-10-01

    Malassezia furfur is a dimorphic yeast that is part of the human skin microflora. This fungus is a pathogen of a certain skin diseases, such as pityriasis versicolor, and in rare cases causes systemic infection in neonates. However, the role of dimorphism in the pathogenicity remains unclear. A modified induction medium (IM) was successfully able to induce mycelial growth of M. furfur under both solid and liquid condition. Filamentous elements with branching hyphae were observed when cultured in the IM. Furthermore, addition of bovine fetus serum into the liquid IM did not promote hyphal formation; on the contrary, it retrograded hyphae to the yeast form. Plate-washing assay showed that M. furfur hyphae did not possess the ability of invasive growth. Secretory proteins from both yeast and hyphal forms were isolated, and lipase and protease activities were analyzed. Intriguingly, the hyphal form showed higher activities than those of the yeast form, particularly the protease activity. PMID:26173769

  15. Comparing Two Forms of Concept Map Critique Activities to Facilitate Knowledge Integration Processes in Evolution Education

    ERIC Educational Resources Information Center

    Schwendimann, Beat A.; Linn, Marcia C.

    2016-01-01

    Concept map activities often lack a subsequent revision step that facilitates knowledge integration. This study compares two collaborative critique activities using a Knowledge Integration Map (KIM), a form of concept map. Four classes of high school biology students (n?=?81) using an online inquiry-based learning unit on evolution were assigned…

  16. Differential cellulolytic activity of native-form and C-terminal tagged-form cellulase derived from coptotermes formosanus and expressed in E. coli

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The endogenous cellulase gene (CfEG3a) of Coptotermes formosanus, an economically important pest termite, was cloned and overexpressed in both native form (nCfEG) and C-terminal His-tagged form (tCfEG) in E.coli. Both forms of recombinant cellulases showed hydrolytic activity on cellulosic substrate...

  17. 77 FR 59739 - Revision to Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-01

    ..., (202) 502-6527, Andrew.Knudsen@ferc.gov . Michael Lacy (Technical Information), Office of Energy Market... throughput that combines interstate and intrastate data.\\11\\ \\8\\ Revision to Form No. 6, 76 FR 46668 (Aug. 3... and the National Propane Gas Association (ATA/ NPGA); Magellan Pipeline, L.P. (Magellan);...

  18. A transcriptionally active form of GAL4 is phosphorylated and associated with GAL80.

    PubMed Central

    Parthun, M R; Jaehning, J A

    1992-01-01

    The GAL4 activator and GAL80 repressor proteins regulate the expression of yeast genes in response to galactose. A complex of the two proteins isolated from glucose-grown cells is inactive in an in vitro transcription reaction but binds DNA and blocks activation by the GAL4-VP16 chimeric activator. The complex purified from galactose-grown cells contains a mixture of phosphorylated and unphosphorylated forms of GAL4. The galactose-induced form of GAL4 activates in vitro transcription to levels similar to those seen with GAL4-VP16. The induced GAL4 complex is indistinguishable in size and apparent shape from the uninduced complex, consistent with a continued association with GAL80. These results confirm in vivo analyses that correlate GAL4 phosphorylation with galactose induction and support a model of transcriptional activation that does not require GAL80 dissociation. Images PMID:1406674

  19. 76 FR 49764 - Notice of FERC Staff Attendance at the Entergy ICT Transmission Planning Summit and Entegry...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy ICT Transmission Planning... Commission's ongoing outreach efforts. Entergy ICT Transmission Planning Summit August 23, 2011 (8 a.m.-5...

  20. 77 FR 58829 - Notice of FERC Staff Attendance at Entergy/Cleco Teleconference on Order No. 1000

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ..., Office of Energy Market Regulation, Federal Energy Regulatory Commission at (202) 502-6083 or peter... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at Entergy/Cleco Teleconference on Order...

  1. 75 FR 41858 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting July 12, 2010. The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend...

  2. 76 FR 13613 - Notice of FERC Staff Attendance at the ICT Stakeholders Policy Committee and Entergy Regional...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the ICT Stakeholders Policy Committee and Entergy Regional State Committee Meetings The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its...

  3. 75 FR 63168 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting and the Entergy Regional State Committee Meeting October 6, 2010. The Federal Energy Regulatory Commission...

  4. 75 FR 23262 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting and the Entergy Regional State Committee Meeting April 26, 2010. The Federal Energy Regulatory Commission hereby...

  5. 76 FR 67727 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and Stakeholder Meeting The Federal Energy Regulatory Commission hereby gives notice that...

  6. 76 FR 62804 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work... outreach efforts. Entergy Regional State Committee Work Group and Stakeholder Meeting October 19, 2011 (9...

  7. 75 FR 42747 - Smart Grid Update; Notice of Commissioner and Staff Attendance at FERC/NARUC Collaborative on...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... Energy Regulatory Commission Smart Grid Update; Notice of Commissioner and Staff Attendance at FERC/NARUC Collaborative on Smart Response Meeting July 15, 2010. The Federal Energy Regulatory Commission hereby gives.../NARUC Collaborative on Smart Response: Sacramento Convention Center, 1400 J Street, Sacramento, CA...

  8. In situ pore-forming alginate hydrogel beads loaded with in situ formed nano-silver and their catalytic activity.

    PubMed

    Wang, Qin; Liu, Shanshan; Wang, Hong; Yang, Yajiang

    2016-05-14

    An aqueous mixture of sodium carbonate (Na2CO3) and sodium alginate (Na-ALG) was added dropwise into an aqueous solution of Ca(NO3)2, leading to the formation of calcium alginate (Ca-ALG) hydrogel beads. Meanwhile Na2CO3 as a pore-forming precursor was transformed in situ into CaCO3 nanoparticles (CaCO3 NPs). SEM images show that CaCO3 NPs aggregates with a size of ∼10 μm were uniformly distributed in the Ca-ALG hydrogel beads. After subsequent erosion using acetic acid, Ca-ALG hydrogel beads with a uniform microporous structure were obtained. The porosity and specific surface area of such in situ pore-formed hydrogel beads are 16 and 14 times higher than those of the beads prepared in the absence of Na2CO3. Additionally, their porous structure can be modulated by varying the amount of Na2CO3. The obtained porous Ca-ALG hydrogel beads were further immersed into an aqueous solution of AgNO3. Under UV irradiation, the Ag(+) ions adsorbed in the Ca-ALG were in situ reduced to Ag nanoparticles (Ag NPs). SEM and TEM images show that Ag NPs with a size of ∼10 nm were uniformly distributed in the matrix of the hydrogel beads. The loading amount of Ag in the beads can also be modulated by varying the amount of Na2CO3. Furthermore, the resultant Ca-ALG beads loaded with Ag NPs (Ag/Ca-ALG) were used as catalysts. Their catalytic activity was evaluated by using the reduction reaction of 4-nitrophenol as a model reaction. The rate constant of the reaction in the presence of dry porous Ag/Ca-ALG beads was found to be 36 times higher than that in the presence of beads prepared in the absence of Na2CO3. Such a high catalytic efficiency can be attributed to their porous structure and consequent high Ag-loading capacity. PMID:27093592

  9. Serine substitution for cysteine residues in levansucrase selectively abolishes levan forming activity.

    PubMed

    Senthilkumar, Velusamy; Busby, Stephen J W; Gunasekaran, Paramasamy; Senthikumar, Velusamy; Bushby, Stephen J W

    2003-10-01

    Levansucrase is responsible for levan formation during sucrose fermentation of Zymomonas mobilis, and this decreases the efficiency of ethanol production. As thiol modifying agents decrease levan formation, a role for cysteine residues in levansucrase activity has been examined using derivatives of Z. mobilis levansucrase that carry serine substitutions of cysteine at positions 121, 151 or 244. These substitutions abolished the levan forming activity of levansucrase whilst only halving its activity in sucrose hydrolysis. Thus, polymerase and hydrolase activities of Z. mobilis levansucrase are separate and have different requirements for the enzyme's cysteine residues. PMID:14584923

  10. [Value of different serological tests in the diagnosis of various forms of active ocular toxoplasmosis].

    PubMed

    Prost, M; Umiński, J

    1992-01-01

    The analysis of results of indirect immunofluorescence and direct agglutination reaction in patients with various forms of ocular toxoplasmosis showed that these reactions have a limited value in diagnostics of this condition. One can define a sure diagnosis of active ocular toxoplasmosis when the titre of these reactions are higher than 512. The ELISA IgM reaction is specific for the active form of this disease. The sensitivity of this reaction amounts 43-50%. In cases of iridocyclitis the results of the serological tests were similar to those of the control group; this confirms the hypothesis that the inflammation is evoked by an allergic reaction. PMID:1635369

  11. Key U. S. gas policy issues await action by FERC during 1991

    SciTech Connect

    Crow, P.

    1991-04-08

    The Federal Energy Regulatory Commission expects to decide several key U.S. natural gas policy issues this year, absent much of the controversy over its actions in recent years designed to inject competition into gas markets. Chairman Martin Alday the his goal is to allow competitive forces to work through the marketplace to maintain the appropriate balance between supply and demand in the pipeline and producer program. He the that this will be accomplished through continued implementation of policies related to construction certificate requirements, rate design, and restructuring pipeline service obligations, and phased deregulation of wellhead prices. FERC is grapping with the issues of pipeline rate design, comparability of service, and pipeline permitting changes while continuing to administer rules governing marketing affiliates and gas inventory charges.

  12. Functional Specificity of the Visual Word Form Area: General Activation for Words and Symbols but Specific Network Activation for Words

    ERIC Educational Resources Information Center

    Reinke, Karen; Fernandes, Myra; Schwindt, Graeme; O'Craven, Kathleen; Grady, Cheryl L.

    2008-01-01

    The functional specificity of the brain region known as the Visual Word Form Area (VWFA) was examined using fMRI. We explored whether this area serves a general role in processing symbolic stimuli, rather than being selective for the processing of words. Brain activity was measured during a visual 1-back task to English words, meaningful symbols…

  13. Process for forming a homogeneous oxide solid phase of catalytically active material

    DOEpatents

    Perry, Dale L.; Russo, Richard E.; Mao, Xianglei

    1995-01-01

    A process is disclosed for forming a homogeneous oxide solid phase reaction product of catalytically active material comprising one or more alkali metals, one or more alkaline earth metals, and one or more Group VIII transition metals. The process comprises reacting together one or more alkali metal oxides and/or salts, one or more alkaline earth metal oxides and/or salts, one or more Group VIII transition metal oxides and/or salts, capable of forming a catalytically active reaction product, in the optional presence of an additional source of oxygen, using a laser beam to ablate from a target such metal compound reactants in the form of a vapor in a deposition chamber, resulting in the deposition, on a heated substrate in the chamber, of the desired oxide phase reaction product. The resulting product may be formed in variable, but reproducible, stoichiometric ratios. The homogeneous oxide solid phase product is useful as a catalyst, and can be produced in many physical forms, including thin films, particulate forms, coatings on catalyst support structures, and coatings on structures used in reaction apparatus in which the reaction product of the invention will serve as a catalyst.

  14. 77 FR 31033 - Agency Information Collection Activities: Form I-589, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-24

    ... published in the Federal Register on March 19, 2012, at 77 FR 16047, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-589... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  15. 77 FR 27474 - Agency Information Collection Activities: Form N-648, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-648... Department of Homeland Security (DHS), U.S. Citizenship and Immigration Services (USCIS) will be submitting.... Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked or required to respond,...

  16. 77 FR 34052 - Agency Information Collection Activities: Form I-102; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-08

    ... was previously published in the Federal Register on February 28, 2012 at 77 FR 12070, allowing for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-102... Document. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will...

  17. 75 FR 65022 - Agency Information Collection Activities: Form I-698, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-21

    .... The information collection was previously published in the Federal Register on June 23, 2010, at 75 FR... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-698... Resident; OMB Control No. 1615-0035. The Department of Homeland Security, U.S. Citizenship and...

  18. 77 FR 34398 - Agency Information Collection Activities: Form N-565, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ..., 2012, at 77 FR 18255, allowing for a 60-day public comment period. USCIS did not receive any comments... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-565... Immigration Services (USCIS) will be submitting the following information collection request to the Office...

  19. 77 FR 3485 - Agency Information Collection Activities: Form I-129F, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-24

    ... Federal Register on October 28, 2011, at 76 FR 66944, allowing for a 60-day public comment period. USCIS... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-129F... Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the following...

  20. 77 FR 27474 - Agency Information Collection Activities: Form I-687; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-10

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-687...-687. The Department Homeland Security (DHS), U.S. Citizenship and Immigration Services (USCIS) has...-687; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be asked...

  1. 77 FR 2560 - Agency Information Collection Activities: Form I-90, Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-18

    ... previously published in the Federal Register on November 15, 2011, at 76 FR 70747, allowing for a 60-day... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-90... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  2. 77 FR 3484 - Agency Information Collection Activities: Form I-914, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-24

    ... Federal Register on October 28, 2011, at 76 FR 66944, allowing for a 60-day public comment period. USCIS... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-914... Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the following...

  3. 76 FR 31971 - Agency Information Collection Activities: Form I-212; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    ... on March 16, 2011, at 76 FR 14419, allowing for a 60-day public comment period. USCIS received no... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-212.... Citizenship and Immigration Services (USCIS) will be submitting the following information collection...

  4. 75 FR 65022 - Agency Information Collection Activities: Form I-751, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-21

    ... collection was previously published in the Federal Register on June 30, 2010, at 75 FR 37821, allowing for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-751.... 1615- 0038. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS)...

  5. 77 FR 34053 - Agency Information Collection Activities: Form I-590, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-08

    ... published in the Federal Register on March 12, 2012, at 77 FR 14535, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-590... of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  6. 76 FR 72209 - Agency Information Collection Activities: Form N-300; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-22

    ... the Federal Register on August 24, 2011 at 76 FR 52961, allowing for a 60-day public comment period... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-300... Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting the...

  7. 77 FR 3486 - Agency Information Collection Activities: Form I-539, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-24

    ... previously published in the Federal Register on October 28, 2011, at 76 FR 66946, allowing for a 60-day... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-539... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  8. 76 FR 28128 - Reports, Forms, and Record Keeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    ... comment period was published on September 13, 2010 (75 FR 55627-55628). DATES: Comments must be submitted... National Highway Traffic Safety Administration Reports, Forms, and Record Keeping Requirements; Agency Information Collection Activity Under OMB Review AGENCY: National Highway Traffic Safety Administration...

  9. 75 FR 52541 - Agency Information Collection Activities: Form I-243, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-26

    ... published in the Federal Register on June 9, 2010, at 75 FR 32799, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-243, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  10. 76 FR 69275 - Agency Information Collection Activities: Form I-192, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-08

    ... the Federal Register on August 12, 2011, at 76 FR 50239, allowing for a 60- day public comment period... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-192, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  11. 77 FR 23734 - Agency Information Collection Activities: Form I-361, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-20

    ..., 2012, at 77 FR 9259, allowing for a 60-day public comment period. USCIS did not receive any comments... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-361, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  12. 75 FR 52541 - Agency Information Collection Activities: Form I-865, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-26

    ... was previously published in the Federal Register on June 9, 2010, at 75 FR 32801, allowing for a 60... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-865, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  13. 76 FR 43335 - Agency Information Collection Activities: Form I-765, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... collection was previously published in the Federal Register on April 19, 2011, at 76 FR 21912 allowing for a... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-765, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice...

  14. 40 CFR 761.205 - Notification of PCB waste activity (EPA Form 7710-53).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Notification of PCB waste activity (EPA Form 7710-53). 761.205 Section 761.205 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) TOXIC SUBSTANCES CONTROL ACT POLYCHLORINATED BIPHENYLS (PCBs) MANUFACTURING, PROCESSING, DISTRIBUTION IN COMMERCE, AND USE PROHIBITIONS...

  15. 75 FR 57480 - Agency Information Collection Activities: Customs Declaration (Form 6059B)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-21

    ... SECURITY U.S. Customs And Border Protection Agency Information Collection Activities: Customs Declaration (Form 6059B) AGENCY: U.S. Customs and Border Protection (CBP), Department of Homeland Security. ACTION... the Customs Declaration. This request for comment is being made pursuant to the Paperwork...

  16. 75 FR 71452 - Agency Information Collection Activities: Customs Declaration (Form 6059B)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-23

    ... published in the Federal Register (75 FR 57480) on September 21, 2010, allowing for a 60-day comment period... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Customs Declaration (Form 6059B) AGENCY: U.S. Customs and Border Protection, Department of Homeland Security. ACTION:...

  17. 76 FR 11805 - Agency Information Collection Activities: Form I-134, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... published in the Federal Register on December 14, 2010, at 75 FR 77891, allowing for a 60-day public comment... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-134... Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS) will be submitting...

  18. 75 FR 71451 - Agency Information Collection Activities: Form N-470, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-23

    ... information collection was previously published in the Federal Register on August 18, 2010, at 75 FR 51096... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-470... Control No. 1615-0056. The Department of Homeland Security, U.S. Citizenship and Immigration...

  19. 76 FR 75892 - Agency Information Collection Activities: Form I-690; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-05

    ... collection notice was previously published in the Federal Register on August 19, 2011, at 76 FR 51996... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-690... 1615-0032. The Department of Homeland Security, U.S. Citizenship and Immigration Services (USCIS)...

  20. 75 FR 12249 - Agency Information Collection Activities: Form I-134, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... the Federal Register on November 12, 2009, at 74 FR 58302 allowing for a 60-day public comment period... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-134... Homeland Security, U.S. Citizenship and Immigration Services (USCIS) has submitted the...

  1. 75 FR 65500 - Agency Information Collection Activities: Form I-360, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-25

    ... information collection was previously published in the Federal Register on June 30, 2010, at 75 FR 37820... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-360... Control No. 1615-0020. The Department of Homeland Security, U.S. Citizenship and Immigration...

  2. 76 FR 37059 - Agency Information Collection Activities: Proposed Collection; Comment Request-Form FNS-339...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    ... Food and Nutrition Service Agency Information Collection Activities: Proposed Collection; Comment Request--Form FNS-339, Federal-State Supplemental Nutrition Program(s) Agreement AGENCY: Food and Nutrition Service, USDA. ACTION: Notice. SUMMARY: In accordance with the Paperwork Reduction Act of...

  3. Chemical forms of selenium affect glutatione peroxidase activity in human Caco-2 cell model

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The bioavailability of selenium (Se) is complicated because there are multiple naturally occurring chemical forms of this element in nature. Assessing the ability of a Se source to restore GPX1 activity in laboratory animals and humans is the most commonly used method. To search for an alternative (...

  4. 75 FR 5099 - Agency Information Collection Activities: Form N-648, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-648... No. 1615-0060. ] The Department of Homeland Security, U.S. Citizenship and Immigration Services... practical utility; (2) Evaluate the accuracy of the agency's estimate of the burden of the collection...

  5. 77 FR 41796 - Agency Information Collection Activities: Monthly Report on Naturalization Papers, Form N-4...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Monthly Report on Naturalization Papers, Form N-4; Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of...

  6. 77 FR 13136 - Agency Information Collection Activities: Form I-134; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-134; Extension of an Existing Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Affidavit of...

  7. Relationship between phosphorus forms and phosphatase activity in soils amended with poultry manure

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Up to 80% of the phosphorus (P) in poultry manure (PM) can be present in organic forms that require mineralization via phosphatase enzymes prior to plant utilization. To determine the correlation between soil P distribution and phosphatase activity we sequentially extracted two Maine soils amended...

  8. 78 FR 46597 - Agency Information Collection Activities: Comment Request for the Production Estimate (2 Forms)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-01

    ....S. Geological Survey Agency Information Collection Activities: Comment Request for the Production...). SUPPLEMENTARY INFORMATION: I. Abstract This collection is needed to provide data on mineral production for... OMB Control Number: 1028-0065. Form Numbers: 9-4042-A and 9-4124-A. Title: Production Estimate....

  9. 76 FR 11807 - Agency Information Collection Activities: Form N-565, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... 75 FR 80835, allowing for a 60-day public comment period. USCIS did not receive any comments for this... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form N-565... Document; OMB Control No. 1615-0091. * * * * * The Department of Homeland Security, U.S. Citizenship...

  10. 75 FR 51094 - Agency Information Collection Activities: Form I-363, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    .... The information collection was previously published in the Federal Register on June 23, 2010, at 75 FR... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-363... Amerasian; OMB Control No. 1615-0022. The Department of Homeland Security, U.S. Citizenship and...

  11. 75 FR 3245 - Agency Information Collection Activities: Aircraft/Vessel Report (Form I-92)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-20

    ... published in the Federal Register (74 FR 54839) on October 23, 2009, allowing for a 60-day comment period... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Aircraft/Vessel... approval in accordance with the Paperwork Reduction Act: Aircraft/ Vessel Report (Form I-92). This is...

  12. 76 FR 2917 - Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-18

    ... proposed information collection was previously published in the Federal Register (75 FR 61508) on October 5... SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68) AGENCY: U.S. Customs and Border Protection, Department of Homeland...

  13. PROCESS FOR THE PRODUCTION OF AN ACTIVATED FORM OF UO$sub 2$

    DOEpatents

    Polissar, M.J.

    1957-09-24

    A process for producing a highly active form of UO/sub 2/ characterized both by rapid oxidation in air and by rapid chlorination with CCl/sub 4/ vapor at an elevated temperature is reported. In accordance with the process, commercial UO/sub 2/, is subjected to a series of oxidation-reduction operations to produce a form of UC/sub 2/ of enhanced reactivity. By treatimg commercial UO/sub 2/ at a temperature between 335 and 485 deg C with methane, then briefly with an oxygen containing gas and followimg this by a second treatment with a methane containing gas, the original relatively stable charge of UO/sub 2/ will be transformed into an active form of UO/sub 2/.

  14. Formation scheme and antioxidant activity of a novel Maillard pigment, pyrrolothiazolate, formed from cysteine and glucose.

    PubMed

    Noda, Kyoko; Terasawa, Naoko; Murata, Masatsune

    2016-06-15

    We recently identified 6-hydroxy-3[R],7a[S]-dimethyl-7-oxo-2,3-dihydropyrrolo[2,1-b]thiazole-3-calboxylic acid, a novel pyrrolothiazole derivative carrying a carboxy group and named pyrrolothiazolate, as a Mallard pigment formed from l-cysteine and d-glucose. Here we described the formation of its enantiomer, the plausible formation scheme of pyrrolothiazolate, and its antioxidant activity. When d-cysteine was used instead of l-cysteine in the reaction mixture, the enantiomer of pyrrolothiazolate was obtained. The carbon at position 1 of glucose was incorporated into two methyl groups of pyrrolothiazolate. The pigment was considered to be formed through 1-deoxyglucosone (1-DG). The dehydrated isomer of 1-DG would be condensed with the thiol and amino groups of cysteine. This condensate was dehydrated and cyclized to form pyrrolothiazolate. This compound was an antioxidant showing radical scavenging activity. PMID:26987433

  15. Activated RSC-nucleosome complex and persistently altered form of the nucleosome.

    PubMed

    Lorch, Y; Cairns, B R; Zhang, M; Kornberg, R D

    1998-07-10

    RSC, an abundant, essential chromatin-remodeling complex, related to SWI/SNF complex, binds nucleosomes and naked DNA with comparable affinities, as shown by gel shift analysis. The RSC-nucleosome complex is converted in the presence of ATP to a slower migrating form. This activated complex exhibits greatly increased susceptibility to endo- and exonucleases but retains a full complement of histones. Activation persists in the absence of ATP, and on removal of RSC, the nucleosome is released in an altered form, with a diminished electrophoretic mobility, greater sedimentation rate, and marked instability at elevated ionic strength. The reaction is reversible in the presence of RSC and ATP, with conversion of the altered form back to the nucleosome. PMID:9674424

  16. Nascent Integrin Adhesions Form on All Matrix Rigidities after Integrin Activation.

    PubMed

    Changede, Rishita; Xu, Xiaochun; Margadant, Felix; Sheetz, Michael P

    2015-12-01

    Integrin adhesions assemble and mature in response to ligand binding and mechanical factors, but the molecular-level organization is not known. We report that ∼100-nm clusters of ∼50 β3-activated integrins form very early adhesions under a wide variety of conditions on RGD surfaces. These adhesions form similarly on fluid and rigid substrates, but most adhesions are transient on rigid substrates. Without talin or actin polymerization, few early adhesions form, but expression of either the talin head or rod domain in talin-depleted cells restores early adhesion formation. Mutation of the integrin binding site in the talin rod decreases cluster size. We suggest that the integrin clusters constitute universal early adhesions and that they are the modular units of cell matrix adhesions. They require the association of activated integrins with cytoplasmic proteins, in particular talin and actin, and cytoskeletal contraction on them causes adhesion maturation for cell motility and growth. PMID:26625956

  17. 76 FR 31972 - Agency Information Collection Activities: Form I-508 and Form I-508F, Extension of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    ..., Exemptions and Immunities; OMB Control No. 1615-0025. The Department of Homeland Security, U.S. Citizenship..., Privileges, Exemptions and Immunities. (3) Agency form number, if any, and the applicable component of...

  18. Binary Toxin Subunits of Lysinibacillus sphaericus Are Monomeric and Form Heterodimers after In Vitro Activation

    PubMed Central

    Choong, Yeu Khai; Torres, Jaume; Boonserm, Panadda

    2016-01-01

    The binary toxin from Lysinibacillus sphaericus has been successfully used for controlling mosquito-transmitted diseases. An activation step shortens both subunits BinA and BinB before their interaction with membranes and internalization in midgut cells, but the precise role of this activation step is unknown. Herein, we show conclusively using three orthogonal biophysical techniques that protoxin subunits form only monomers in aqueous solution. However, in vitro activated toxins readily form heterodimers. This oligomeric state did not change after incubation of these heterodimers with detergent. These results are consistent with the evidence that maximal toxicity in mosquito larvae is achieved when the two subunits, BinA and BinB, are in a 1:1 molar ratio, and directly link proteolytic activation to heterodimerization. Formation of a heterodimer must thus be necessary for subsequent steps, e.g., interaction with membranes, or with a suitable receptor in susceptible mosquito species. Lastly, despite existing similarities between BinB C-terminal domain with domains 3 and 4 of pore-forming aerolysin, no aerolysin-like SDS-resistant heptameric oligomers were observed when the activated Bin subunits were incubated in the presence of detergents or lipidic membranes. PMID:27341696

  19. Cytochrome P450-mediated activation of the fragrance compound geraniol forms potent contact allergens

    SciTech Connect

    Hagvall, Lina; Baron, Jens Malte; Boerje, Anna; Weidolf, Lars; Merk, Hans; Karlberg, Ann-Therese

    2008-12-01

    Contact sensitization is caused by low molecular weight compounds which penetrate the skin and bind to protein. In many cases, these compounds are activated to reactive species, either by autoxidation on exposure to air or by metabolic activation in the skin. Geraniol, a widely used fragrance chemical, is considered to be a weak allergen, although its chemical structure does not indicate it to be a contact sensitizer. We have shown that geraniol autoxidizes and forms allergenic oxidation products. In the literature, it is suggested but not shown that geraniol could be metabolically activated to geranial. Previously, a skin-like CYP cocktail consisting of cutaneous CYP isoenzymes, was developed as a model system to study cutaneous metabolism. In the present study, we used this system to investigate CYP-mediated activation of geraniol. In incubations with the skin-like CYP cocktail, geranial, neral, 2,3-epoxygeraniol, 6,7-epoxygeraniol and 6,7-epoxygeranial were identified. Geranial was the main metabolite formed followed by 6,7-epoxygeraniol. The allergenic activities of the identified metabolites were determined in the murine local lymph node assay (LLNA). Geranial, neral and 6,7-epoxygeraniol were shown to be moderate sensitizers, and 6,7-epoxygeranial a strong sensitizer. Of the isoenzymes studied, CYP2B6, CYP1A1 and CYP3A5 showed high activities. It is likely that CYP1A1 and CYP3A5 are mainly responsible for the metabolic activation of geraniol in the skin, as they are expressed constitutively at significantly higher levels than CYP2B6. Thus, geraniol is activated through both autoxidation and metabolism. The allergens geranial and neral are formed via both oxidation mechanisms, thereby playing a large role in the sensitization to geraniol.

  20. [Biophysical aspects of biological activity structure--strain calcium carbonat in micellar form].

    PubMed

    Ivanov, V I; Stekhin, A A; Iakovleva, G V; Savostikova, O N; Alekseeva, A V; P'ianzina, I P

    2013-01-01

    Results of the study of electrochemical and structural state of phase of associated water in the solutions of structurally stressed calcium carbonate in the micellar form are reported. On the base on the comparison of structural--physical changes of activated water with the data on the activity of bioluminiscentic "Ecolyum" microorganisms in their noncontact activation the electronic mechanism of the effect of activated water on cellular metabolism is substantiated The use of "Micellate of calcium" possessing non-contact electron-donor action on cellular structures was shown to permit to compensate the deficit of electrons and thereby to restore the activities of reductases and iron-containing peptides required for the production of regulatory ROS and alteration in redox state of the intracellular environment. PMID:24624817

  1. Integration of active pharmaceutical ingredient solid form selection and particle engineering into drug product design.

    PubMed

    Ticehurst, Martyn David; Marziano, Ivan

    2015-06-01

    This review seeks to offer a broad perspective that encompasses an understanding of the drug product attributes affected by active pharmaceutical ingredient (API) physical properties, their link to solid form selection and the role of particle engineering. While the crucial role of active pharmaceutical ingredient (API) solid form selection is universally acknowledged in the pharmaceutical industry, the value of increasing effort to understanding the link between solid form, API physical properties and drug product formulation and manufacture is now also being recognised. A truly holistic strategy for drug product development should focus on connecting solid form selection, particle engineering and formulation design to both exploit opportunities to access simpler manufacturing operations and prevent failures. Modelling and predictive tools that assist in establishing these links early in product development are discussed. In addition, the potential for differences between the ingoing API physical properties and those in the final product caused by drug product processing is considered. The focus of this review is on oral solid dosage forms and dry powder inhaler products for lung delivery. PMID:25677227

  2. [Effect of different nitrogen forms and ratio on growth and active ingredient content of Platycodon grandiflorum].

    PubMed

    Duan, Yun-jing; Wang, Kang-cai; Niu, Ling-hui; Li, Ke; Su, Yun-yun

    2015-10-01

    To providing evidence about nitrogen adequate application of Platycodon grandiflorum, the pot culture experiment was conducted to study the effect of nitrogen on the growth, physiological metabolism and the quality of P. grandiflorum. The activity of NR, GS and SOD, POD and CAT were determined. And the nitrate and ammonium nitrogen content, photosynthetic characteristics, active components of P. grandiflorum were determined. The results showed that the nitrate nitrogen content and P. biomass reached its maximum value, when NH4(+)-N/NO3(-) -N was 0: 100, the activity of NR. The activity of GS was the highest at the NH4(+) -N/NO3(-) -N ratio of 25:75 and ammonium nitrogen content was the highest at 75:25. The activity of SOD decreased and then increased with the increasing of NO3(-) -N. At the NH4(+) -N/NO3(-) -N ratio of 25: 75, the activity of CAT had its maximum value and the content of MDA had the minimum value. At the same time, the content of platycodon D was the highest at this treatment. The studies had shown that different nitrogen forms and ratio had a significant effect on the characteristics of photosynthetic physiology, nitrogen metabolism and resistance adjustment, growth and the quality of P. grandiflorum. The NH4(+) -N/NO3(-) -N ratio of 25: 75 was a suitable ratio of nitrogen forms for the growth of P. Grandiflorum and accumulating the content of platycodon D. PMID:26975097

  3. An ELISA method detecting the active form of suPAR.

    PubMed

    Zhou, Xiaolei; Xu, Mingming; Huang, Hailong; Mazar, Andrew; Iqbal, Zafar; Yuan, Cai; Huang, Mingdong

    2016-11-01

    Urokinase plasminogen activator receptor (uPAR) exists in a number of formats in human plasma, including soluble uPAR (suPAR) and uPAR fragments. We developed an ELISA method to detect specifically the active form suPAR, which binds to its natural ligand uPA. The intra CV and inter CV of this ELISA assay is 8.5% and 9.6% respectively, and the assay can recover 99.74% of added recombinant suPAR from 10% plasma. This assay is quite sensitive, capable of detecting down to 15pg/ml of suPAR, and can measure suPAR concentrations in the range of 0.031-8ng/ml with high linear relationship. Plasma samples from pregnant women were also measured for the active form of suPAR with this assay, giving an averaged level of 1.39ng/ml, slightly higher than the level of pooled plasma from healthy donors (0.96ng/ml). This study demonstrates the feasibility to measure the active form of suPAR, which will likely have value in clinical applications. PMID:27591605

  4. Crystallization and preliminary analysis of active nitroalkane oxidase in three crystal forms.

    PubMed

    Nagpal, Akanksha; Valley, Michael P; Fitzpatrick, Paul F; Orville, Allen M

    2004-08-01

    Nitroalkane oxidase (NAO), a flavoprotein cloned and purified from Fusarium oxysporum, catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones, with the production of H2O2 and nitrite. In this paper, the crystallization and preliminary X-ray data analysis of three crystal forms of active nitroalkane oxidase are described. The first crystal form belongs to a trigonal space group (either P3(1)21 or P3(2)21, with unit-cell parameters a = b = 103.8, c = 487.0 A) and diffracts to at least 1.6 A resolution. Several data sets were collected using 2theta and kappa geometry in order to obtain a complete data set to 2.07 A resolution. Solvent-content and Matthews coefficient analysis suggests that crystal form 1 contains two homotetramers per asymmetric unit. Crystal form 2 (P2(1)2(1)2(1); a = 147.3, b = 153.5, c = 169.5 A) and crystal form 3 (P3(1) or P3(2); a = b = 108.9, c = 342.5 A) are obtained from slightly different conditions and also contain two homotetramers per asymmetric unit, but have different solvent contents. A three-wavelength MAD data set was collected from selenomethionine-enriched NAO (SeMet-NAO) in crystal form 3 and will be used for phasing. PMID:15272176

  5. 76 FR 53144 - Agency Information Collection Activities: Form I-508 and Form I-508F, Extension of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-25

    ... information collection was previously published in the Federal Register on June 2, 2011, at 76 FR 31972..., Exemptions and Immunities. The Department of Homeland Security, U.S. Citizenship and Immigration Services..., Privileges, Exemptions and Immunities. (3) Agency form number, if any, and the applicable component of...

  6. Cyclotron production of ``very high specific activity'' platinum radiotracers in No Carrier Added form

    NASA Astrophysics Data System (ADS)

    Birattari, C.; Bonardi, M.; Groppi, F.; Gini, L.; Gallorini, M.; Sabbioni, E.; Stroosnijder, M. F.

    2001-12-01

    At the "Radiochemistry Laboratory" of Accelerators and Applied Superconductivity Laboratory, LASA, several production and quality assurance methods for short-lived and high specific activity radionuclides, have been developed. Presently, the irradiations are carried out at the Scanditronix MC40 cyclotron (K=38; p, d, He-4 and He-3) of JRC-Ispra, Italy, of the European Community, while both chemical purity and specific activity determination are carried out at the TRIGA MARK II research reactor of University of Pavia and at LASA itself. In order to optimize the irradiation conditions for platinum radiotracer production, both thin- and thick-target excitation function of natOs(α,xn) nuclear reactions were measured. A very selective radiochemical separation to obtain Pt radiotracers in No Carrier Added form, has been developed. Both real specific activity and chemical purity of radiotracer, have been determined by neutron activation analysis and atomic absorption spectrometry. An Isotopic Dilution Factor (IDF) of the order of 50 is achieved.

  7. Linear Closed-form Solution and Finite-element Analysis of an Active Tensegrity Unit

    NASA Astrophysics Data System (ADS)

    Kmeť, Stanislav; Platko, Peter

    2012-11-01

    Results of the linear closed form solution of an active or adaptive tensegrity unit, as well as its numerical analysis using finite element method are presented in the paper. The shape of the unit is an octahedral cell with a square base and it is formed by thirteen members (four bottom and four top cables, four edge struts and one central strut). The central strut is designed as an actuator that allows for an adjustment of the shape of the unit which leads to changes of tensile forces in the cables. Due to the diagonal symmetry of the 3D tensegrity unit the closed-form analysis is based on the 2D solution of the equivalent planar biconvex cable system with one central strut under a vertical point load.

  8. Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase.

    PubMed

    Johnston, Esther M; Dell'Acqua, Simone; Ramos, Susana; Pauleta, Sofia R; Moura, Isabel; Solomon, Edward I

    2014-01-15

    N2OR has been found to have two structural forms of its tetranuclear copper active site, the 4CuS Cu(Z)* form and the 4Cu2S Cu(Z) form. EPR, resonance Raman, and MCD spectroscopies have been used to determine the redox states of these sites under different reductant conditions, showing that the Cu(Z)* site accesses the 1-hole and fully reduced redox states, while the Cu(Z) site accesses the 2-hole and 1-hole redox states. Single-turnover reactions of N2OR for Cu(Z) and Cu(Z)* poised in these redox states and steady-state turnover assays with different proportions of Cu(Z) and Cu(Z)* show that only fully reduced Cu(Z)* is catalytically competent in rapid turnover with N2O. PMID:24364717

  9. Active heterodimers are formed from human DNA topoisomerase II alpha and II beta isoforms.

    PubMed Central

    Biersack, H; Jensen, S; Gromova, I; Nielsen, I S; Westergaard, O; Andersen, A H

    1996-01-01

    DNA topoisomerase II is a nuclear enzyme essential for chromosome dynamics and DNA metabolism. In mammalian cells, two genetically and biochemically distinct topoisomerase II forms exist, which are designated topoisomerase II alpha and topoisomerase II beta. In our studies of human topoisomerase II, we have found that a substantial fraction of the enzyme exists as alpha/beta heterodimers in HeLa cells. The ability to form heterodimers was verified when human topoisomerases II alpha and II beta were coexpressed in yeast and investigated in a dimerization assay. Analysis of purified heterodimers shows that these enzymes maintain topoisomerase II specific catalytic activities. The natural existence of an active heterodimeric subclass of topoisomerase II merits attention whenever topoisomerases II alpha and II beta function, localization, and cell cycle regulation are investigated. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:8710863

  10. Role of drop distortion in enhancing the lightning activity in clouds formed over cities

    NASA Astrophysics Data System (ADS)

    Bhalwankar, Rohini; Kamra, A. K.

    2013-03-01

    Atmospheric pollutants can modify the electrification and lightning activity in thunderclouds. Laboratory simulation experiments show that distortion of water drops is more when drops are formed from water polluted with Sulfate/Nitrate salts than that from distilled water and the difference in distortions is more in a higher electric field. Further, the polluted water drops falling in a horizontal electric field can trigger a discharge on their surface and the discharge can propagate as a streamer in lower electric fields as compared to that from distilled water drops. The difference in electrical conductivities of polluted and unpolluted water drops is most likely the key factor for manifestation of these differences. It is proposed that the enhanced distortion of polluted drops coupled with the change in their characteristics to trigger and propagate a discharge in lower electric fields may significantly contribute to the enhancement of lightning activity observed in clouds formed over big cities.

  11. Protease-Activated Pore-Forming Peptides for the Treatment and Imaging of Prostate Cancer

    PubMed Central

    LeBeau, Aaron M.; Denmeade, Samuel R.

    2015-01-01

    A common hallmark of cancers with highly aggressive phenotypes is increased proteolysis in the tumor and the surrounding microenvironment. Prostate cancer has a number of proteases uniquely associated with it that may play various important roles in disease progression. In this report, we utilize the peritumoral proteolytic activity of prostate cancer to activate engineered peptide constructs for the treatment and noninvasive imaging of prostate cancer. Using a modular "propeptide" approach, a cationic diastereomeric pore-forming peptide domain was linked to an inactivating acidic peptide domain. The inactivating acidic peptide domain was engineered to be a cleavable substrate for the secreted serine protease prostate-specific antigen (PSA) or the transmembrane metalloprotease prostate-specific membrane antigen (PSMA). The propeptides were then evaluated in a direct comparison study. Both the PSA and PSMA activated propeptides were found to be cytotoxic to prostate cancer cells in vitro. In vivo, however, treatment of LNCaP and CWR22Rv1 xenografts with the PSMA propeptide resulted in a pronounced cytostatic effect when compared with xenografts treated with the PSA propeptide or the cationic diastereomeric peptide alone. The PSMA activated propeptide also proved to be an effective optical imaging probe in vivo when labeled with a near-infrared fluorophore. These data suggest that protease-activated pore-forming peptides could potentially be used for both imaging and treating prostate cancer. PMID:25537662

  12. Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase.

    PubMed

    Zimoch-Korzycka, Anna; Bobak, Łukasz; Jarmoluk, Andrzej

    2016-01-01

    The aim of this study was to evaluate the impact of cellulase (C) on the biological activity of chitosan/hydroxypropyl methylcellulose (CH/HPMC) film-forming hydrosols. The hydrolytic activity of cellulase in two concentrations (0.05% and 0.1%) was verified by determination of the progress of polysaccharide hydrolysis, based on viscosity measurement and reducing sugar-ends assay. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging effect, the ferric reducing antioxidant power (FRAP), and microbial reduction of Pseudomonas fluorescens, Yersinia enterocolitica, Bacillus cereus, and Staphylococcus aureus were studied. During the first 3 h of reaction, relative reducing sugar concentration increased progressively, and viscosity decreased rapidly. With increasing amount of enzyme from 0.05% to 0.1%, the reducing sugar concentration increased, and the viscosity decreased significantly. The scavenging effect of film-forming solutions was improved from 7.6% at time 0 and without enzyme to 52.1% for 0.1% cellulase after 20 h of reaction. A significant effect of cellulase addition and reaction time on antioxidant power of the tested film-forming solutions was also reported. Film-forming hydrosols with cellulase exhibited a bacteriostatic effect on all tested bacteria, causing a total reduction. PMID:27608008

  13. The chemokine system in diverse forms of macrophage activation and polarization.

    PubMed

    Mantovani, Alberto; Sica, Antonio; Sozzani, Silvano; Allavena, Paola; Vecchi, Annunciata; Locati, Massimo

    2004-12-01

    Plasticity and functional polarization are hallmarks of the mononuclear phagocyte system. Here we review emerging key properties of different forms of macrophage activation and polarization (M1, M2a, M2b, M2c), which represent extremes of a continuum. In particular, recent evidence suggests that differential modulation of the chemokine system integrates polarized macrophages in pathways of resistance to, or promotion of, microbial pathogens and tumors, or immunoregulation, tissue repair and remodeling. PMID:15530839

  14. Validity and Reliability of International Physical Activity Questionnaire-Short Form in Chinese Youth

    ERIC Educational Resources Information Center

    Wang, Chao; Chen, Peijie; Zhuang, Jie

    2013-01-01

    Purpose: The psychometric profiles of the widely used International Physical Activity Questionnaire-Short Form (IPAQ-SF) in Chinese youth have not been reported. The purpose of this study was to examine the validity and reliability of the IPAQ-SF using a sample of Chinese youth. Method: One thousand and twenty-one youth (M[subscript age] = 14.26 ±…

  15. The Prodomain-bound Form of Bone Morphogenetic Protein 10 Is Biologically Active on Endothelial Cells*

    PubMed Central

    Jiang, He; Salmon, Richard M.; Upton, Paul D.; Wei, Zhenquan; Lawera, Aleksandra; Davenport, Anthony P.; Morrell, Nicholas W.; Li, Wei

    2016-01-01

    BMP10 is highly expressed in the developing heart and plays essential roles in cardiogenesis. BMP10 deletion in mice results in embryonic lethality because of impaired cardiac development. In adults, BMP10 expression is restricted to the right atrium, though ventricular hypertrophy is accompanied by increased BMP10 expression in a rat hypertension model. However, reports of BMP10 activity in the circulation are inconclusive. In particular, it is not known whether in vivo secreted BMP10 is active or whether additional factors are required to achieve its bioactivity. It has been shown that high-affinity binding of the BMP10 prodomain to the mature ligand inhibits BMP10 signaling activity in C2C12 cells, and it was proposed that prodomain-bound BMP10 (pBMP10) complex is latent. In this study, we demonstrated that the BMP10 prodomain did not inhibit BMP10 signaling activity in multiple endothelial cells, and that recombinant human pBMP10 complex, expressed in mammalian cells and purified under native conditions, was fully active. In addition, both BMP10 in human plasma and BMP10 secreted from the mouse right atrium were fully active. Finally, we confirmed that active BMP10 secreted from mouse right atrium was in the prodomain-bound form. Our data suggest that circulating BMP10 in adults is fully active and that the reported vascular quiescence function of BMP10 in vivo is due to the direct activity of pBMP10 and does not require an additional activation step. Moreover, being an active ligand, recombinant pBMP10 may have therapeutic potential as an endothelial-selective BMP ligand, in conditions characterized by loss of BMP9/10 signaling. PMID:26631724

  16. Mechanistic insights into the first Lygus-active β-pore forming protein.

    PubMed

    Jerga, Agoston; Chen, Danqi; Zhang, Chunfen; Fu, Jinping; Kouadio, Jean-Louis K; Wang, Yanfei; Duff, Stephen M G; Howard, Jennifer E; Rydel, Timothy J; Evdokimov, Artem G; Ramaseshadri, Parthasarathy; Evans, Adam; Bolognesi, Renata; Park, Yoonseong; Haas, Jeffrey A

    2016-06-15

    The cotton pests Lygus hesperus and Lygus lineolaris can be controlled by expressing Cry51Aa2.834_16 in cotton. Insecticidal activity of pore-forming proteins is generally associated with damage to the midgut epithelium due to pores, and their biological specificity results from a set of key determinants including proteolytic activation and receptor binding. We conducted mechanistic studies to gain insight into how the first Lygus-active β-pore forming protein variant functions. Biophysical characterization revealed that the full-length Cry51Aa2.834_16 was a stable dimer in solution, and when exposed to Lygus saliva or to trypsin, the protein underwent proteolytic cleavage at the C-terminus of each of the subunits, resulting in dissociation of the dimer to two separate monomers. The monomer showed tight binding to a specific protein in Lygus brush border membranes, and also formed a membrane-associated oligomeric complex both in vitro and in vivo. Chemically cross-linking the β-hairpin to the Cry51Aa2.834_16 body rendered the protein inactive, but still competent to compete for binding sites with the native protein in vivo. Our study suggests that disassociation of the Cry51Aa2.834_16 dimer into monomeric units with unoccupied head-region and sterically unhindered β-hairpin is required for brush border membrane binding, oligomerization, and the subsequent steps leading to insect mortality. PMID:27001423

  17. 77 FR 9259 - Agency Information Collection Activities: Forms G-1041 and G-1041A, Extension of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-16

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Forms G-1041... Notice of Information Collection Under Review: Forms G- 1041 and G-1041A, Genealogy Index Search Request... evaluating whether to revise forms G-1041 and G-1041A. Should USCIS decide to revise forms G- 1041 and...

  18. MAVS Forms Functional Prion-Like Aggregates To Activate and Propagate Antiviral Innate Immune Response

    PubMed Central

    Hou, Fajian; Sun, Lijun; Zheng, Hui; Skaug, Brian; Jiang, Qiu-Xing; Chen, Zhijian J.

    2011-01-01

    SUMMARY In response to viral infection, RIG-I–like RNA helicases bind to viral RNA and activate the mitochondrial protein MAVS, which in turn activates the transcription factors IRF3 and NF-κB to induce type-I interferons. We have previously shown that RIG-I binds to unanchored lysine-63 (K63) polyubiquitin chains and that this binding is important for MAVS activation; however, the mechanism underlying MAVS activation is not understood. Here we show that viral infection induces the formation of very large MAVS aggregates, which potently activate IRF3 in the cytosol. We find that a fraction of recombinant MAVS protein forms fibrils capable of activating IRF3. Remarkably, the MAVS fibrils behave like prions and effectively convert endogenous MAVS into functional aggregates. We also show that, in the presence of K63 ubiquitin chains, RIG-I catalyzes the conversion of MAVS on the mitochondrial membrane to prion-like aggregates. These results suggest that a prion-like conformational switch of MAVS activates and propagates the antiviral signaling cascade. PMID:21782231

  19. Brain activation patterns resulting from learning letter forms through active self-production and passive observation in young children

    PubMed Central

    Kersey, Alyssa J.; James, Karin H.

    2013-01-01

    Although previous literature suggests that writing practice facilitates neural specialization for letters, it is unclear if this facilitation is driven by the perceptual feedback from the act of writing or the actual execution of the motor act. The present study addresses this issue by measuring the change in BOLD signal in response to hand-printed letters, unlearned cursive letters, and cursive letters that 7-year-old children learned actively, by writing, and passively, by observing an experimenter write. Brain activation was assessed using fMRI while perceiving letters—in both cursive and manuscript forms. Results showed that active training led to increased recruitment of the sensori-motor network associated with letter perception as well as the insula and claustrum, but passive observation did not. This suggests that perceptual networks for newly learned cursive letters are driven by motor execution rather than by perceptual feedback. PMID:24069007

  20. Theoretical studies of chemical reactivity of metabolically activated forms of aromatic amines toward DNA.

    PubMed

    Shamovsky, Igor; Ripa, Lena; Blomberg, Niklas; Eriksson, Leif A; Hansen, Peter; Mee, Christine; Tyrchan, Christian; O'Donovan, Mike; Sjö, Peter

    2012-10-15

    The metabolism of aromatic and heteroaromatic amines (ArNH₂) results in nitrenium ions (ArNH⁺) that modify nucleobases of DNA, primarily deoxyguanosine (dG), by forming dG-C8 adducts. The activated amine nitrogen in ArNH⁺ reacts with the C8 of dG, which gives rise to mutations in DNA. For the most mutagenic ArNH₂, including the majority of known genotoxic carcinogens, the stability of ArNH⁺ is of intermediate magnitude. To understand the origin of this observation as well as the specificity of reactions of ArNH⁺ with guanines in DNA, we investigated the chemical reactivity of the metabolically activated forms of ArNH₂, that is, ArNHOH and ArNHOAc, toward 9-methylguanine by DFT calculations. The chemical reactivity of these forms is determined by the rate constants of two consecutive reactions leading to cationic guanine intermediates. The formation of ArNH⁺ accelerates with resonance stabilization of ArNH⁺, whereas the formed ArNH⁺ reacts with guanine derivatives with the constant diffusion-limited rate until the reaction slows down when ArNH⁺ is about 20 kcal/mol more stable than PhNH⁺. At this point, ArNHOH and ArNHOAc show maximum reactivity. The lowest activation energy of the reaction of ArNH⁺ with 9-methylguanine corresponds to the charge-transfer π-stacked transition state (π-TS) that leads to the direct formation of the C8 intermediate. The predicted activation barriers of this reaction match the observed absolute rate constants for a number of ArNH⁺. We demonstrate that the mutagenic potency of ArNH₂ correlates with the rate of formation and the chemical reactivity of the metabolically activated forms toward the C8 atom of dG. On the basis of geometric consideration of the π-TS complex made of genotoxic compounds with long aromatic systems, we propose that precovalent intercalation in DNA is not an essential step in the genotoxicity pathway of ArNH₂. The mechanism-based reasoning suggests rational design strategies to

  1. 76 FR 36909 - Commission Information Collection Activities (FERC-549B); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-23

    ... regulations regarding scheduling, segmentation and flexible point rights, penalties, and reporting... needed to make informed decisions in a competitive market as well as to improve shipper's and the Commission's ability to monitor the market for potential abuses. Peak Day Annual Capacity Report Under...

  2. 77 FR 58116 - Commission Information Collection Activities (FERC-732); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-19

    ... Rights in Organized Electricity Markets. DATES: Comments on the collection of information are due... Rights in Organized Electricity Markets. OMB Control No.:1902-0245. Type of Request: 18 CFR part 42... transmission organization that is a public utility with one or more organized electricity markets to...

  3. 77 FR 73630 - Commission Information Collection Activities (FERC-732); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ... explained below. The Commission issued a Notice in the Federal Register (77 FR 58116, 9/19/2012) requesting... and Tariffs: Long-Term Firm Transmission Rights in Organized Electricity Markets) to the Office of... Tariffs: Long-Term Firm Transmission Rights in Organized Electricity Markets. OMB Control No.:...

  4. 76 FR 46783 - Commission Information Collection Activities (FERC-549); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    .... Improved storage technology and the increased use of natural gas in industry and electric generation have... orders to which the price data may be relevant. \\2\\ 18 CFR 1c.1 and 1c.2, 71 FR 4,244 (2006). Failure to... relatively constant throughout the year, while many uses of natural gas, residential space heating...

  5. 75 FR 65618 - Commission Information Collection Activities (FERC-725B); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-26

    ... requirements to safeguard critical cyber assets.\\4\\ These standards help protect the nation's Bulk-Power System against potential disruptions from cyber attacks.\\5\\ \\3\\ CIP-002-1, CIP-003-1, CIP-004-1, CIP-005-1, CIP... Cyber Asset Identification. Security Management Controls. Personnel and Training. Electronic...

  6. 76 FR 52646 - Commision Information Collection Activities [FERC-588]; Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ..., such as administrative costs and the cost for information technology. Indirect or overhead costs are... to the Commission as explained below. The Commission issued a Notice in the Federal Register (76 FR...) annually (1) respondent (2) response (3) x (2) x (3) 8 1 8 64 The estimated total cost to respondents is...

  7. 76 FR 34216 - Commission Information Collection Activities (Ferc-521), Proposed Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-13

    ..., such as administrative costs and the cost for information technology. Indirect or overhead costs are... various downstream beneficiaries. A headwater benefits charge and the cost incurred by the Commission to... U.S. Treasury. The regulations provide for apportionment of these costs between the...

  8. 76 FR 43996 - Commission Information Collection Activities (FERC-510); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-22

    ... attributable to providing this information, such as administrative costs and the cost for information...) (2) (3) (1)x(2)x(3) 16 1 10 160 ] Estimated cost burden to respondents is $10,952 (160 hours/2080... cost per respondent is $685 (rounded). The reporting burden includes the total time, effort,...

  9. 75 FR 57744 - Commission Information Collection Activities (FERC-555); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-22

    ... to providing this information, such as administrative costs and the cost for information technology...). Further, the previous estimate reported the average annual cost per respondent and total annual cost as... more than 10,000 hours per year to comply. The estimated total annual cost burden to...

  10. 76 FR 34689 - Commission Information Collection Activities [FERC-583], Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-14

    ... government administrative costs and for the use of government dams. The Commission implements these filing... attributable to providing this information, such as ] administrative costs and the cost for information... hydropower licensees for, among other things, the cost of administering part I of the FPA and for the use...

  11. 77 FR 2963 - Commission Information Collection Activities (FERC-725F); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... ERO and approved by the Commission apply to users, owners and operators of the Bulk-Power System as... generator operators (generally nuclear power plant owners and operators, including licensees) and... owners and generator operators. Reliability Standard NUC-001-2 requires a nuclear power plant...

  12. 76 FR 12090 - Commission Information Collection Activities (FERC-73); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    ... Energy Organization Act, 42 U.S.C. 7155 and 7172, and Executive Order No. 12009, 42 FR 46277 (September.... The Commission implements these filings in 18 CFR parts 347 and 357. Action: The Commission...

  13. 76 FR 18743 - Commission Information Collection Activities (FERC-603); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    ... Information Act (5 USC 552). On February, 21, 2003, the Commission issued Order No. 630 (66 FR 52917) to... requester's need for the information against the sensitivity of the information. The compliance with...

  14. 78 FR 30912 - Commission Information Collection Activities (FERC-582); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-23

    ...: Electric Fees, Annual Charges, Waivers, and Exemptions. OMB Control No.: 1902-0132. Type of Request: Three... addition, the Omnibus Budget Reconciliation Act of 1986 (OBRA) \\4\\ authorizes the Commission to assess...

  15. 76 FR 28014 - Commission Information Collection Activities (FERC-600); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    .... The data in complaints filed by interested/affected parties regarding oil and natural gas pipeline... concerns. In order to make a better determination, it is important to know the specifics of any oil, gas..., such as administrative costs and the cost for information technology. Indirect or overhead costs...

  16. 78 FR 29359 - Commission Information Collection Activities (Ferc-604); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-20

    ... Respondents: Public utilities, natural gas companies, and oil pipeline companies. Estimate of Annual Burden \\1...) = (C) (D) (C) x (D) Public utilities and licensees, natural gas companies, and oil \\2\\ 25 1 25 1.5 37.5... of information technology. Dated: May 13, 2013. Kimberly D. Bose, Secretary. BILLING CODE 6717-01-P...

  17. 78 FR 61983 - Commission Information Collection Activities (FERC-555); Comment Request; Extension

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    2013-10-09

    ... apply to the regulated public utilities, natural gas and oil pipeline companies subject to the... regulations for public utilities and licensees as well as natural gas and oil pipeline companies. These..., Natural Gas and Oil Pipeline Companies Number of Average burden Number of respondents responses per...

  18. 76 FR 67158 - Commission Information Collection Activities (FERC-725F); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... Modernization Act of 2005, which is Title XII, Subtitle A, of the Energy Policy Act of 2005 (EPAct 2005), was enacted into law.\\1\\ EPAct 2005 added a new section 215 to the FPA, which required a...

  19. 75 FR 7465 - Commission Information Collection Activities (FERC-730); Comment Request; Submitted for OMB Review

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    2010-02-19

    ... in response to the Federal Register notice (74 FR 59146, 11/17/2009) and has noted that in its.... Congress enacted section 1241 of the Energy Policy Act of 2005 (EPAct 2005), adding a new section 219 to... cost of delivered power through reducing transmission congestion. In response to EPAct 2005, in...

  20. 78 FR 63172 - Commission Information Collection Activities (FERC-733); Comment Request

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    2013-10-23

    ..., including those available from all consumer classes. Specifically, EPAct 2005 Section 1252(e)(3) requires...) (EPAct 2005). (A) Saturation and penetration rate of advanced meters and communications technologies... programs. Because DADS does not currently collect and report data which is specifically required by...

  1. 76 FR 55370 - Commission Information Collection Activities (FERC-549B); Comment Request; Submitted for OMB Review

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    2011-09-07

    ... Federal Register notice of June 23, 2011 (76 FR 36909) and has made this notation in its submission to OMB... capacity. Pipelines posted available firm and interruptible capacity information on their electronic... rate schedule under which service is rendered, the contract effective date, the contract...

  2. 76 FR 45237 - Commission Information Collection Activities (FERC-600); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... Federal Register notice of May 13, 2011 (76 FR 28014) and has made this notation in its submission to OMB.... * * * In Order No. 602, 64 FR 17087 (April 8, 1999), the Commission revised its regulations governing... oil pipelines, Part 1 of the Interstate Commerce Act (ICA), sections 1, 6 and 15 (re-codified by...

  3. 76 FR 76702 - Commission Information Collection Activities, Proposed Collection (FERC-550); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-08

    ... Executive Order No. 12009, 42 FR 46267 (September 17, 1977). 18 CFR Parts 341-348 specifies the filing...-0089), to implement the statutory provisions of Parts 1, 6, and 15 of the Interstate Commerce Act...

  4. A comparison of the efficiency of G protein activation by ligand-free and light-activated forms of rhodopsin.

    PubMed Central

    Melia, T J; Cowan, C W; Angleson, J K; Wensel, T G

    1997-01-01

    Activation of the photoreceptor G protein transducin (Gt) by opsin, the ligand-free form of rhodopsin, was measured using rod outer segment membranes with densities of opsin and Gt similar to those found in rod cells. When GTPgammaS was used as the activating nucleotide, opsin catalyzed transducin activation with an exponential time course with a rate constant k(act) on the order of 2 x 10(-3)s(-1). Comparison under these conditions to activation by flash-generated metarhodopsin II (MII) revealed that opsin- and R*-catalyzed activation showed similar kinetics when MII was present at a surface density approximately 10(-6) lower than that of opsin. Thus, in contrast to some previous reports, we find that the catalytic potency of opsin is only approximately 10(-6) that of MII. In the presence of residual retinaldehyde-derived species present in membranes treated with hydroxylamine after bleaching, the apparent k(act) observed was much higher than that for opsin, suggesting a possible explanation for previous reports of more efficient activation by opsin. These results are important for considering the possible role of opsin in the diverse phenomena in which it has been suggested to play a key role, such as bleaching desensitization and retinal degeneration induced by continuous light or vitamin A deprivation. PMID:9414230

  5. Implications for the active form of human insulin based on the structural convergence of highly active hormone analogues

    PubMed Central

    Jiráček, Jiří; Žáková, Lenka; Antolíková, Emília; Watson, Christopher J.; Turkenburg, Johan P.; Dodson, Guy G.; Brzozowski, Andrzej M.

    2010-01-01

    Insulin is a key protein hormone that regulates blood glucose levels and, thus, has widespread impact on lipid and protein metabolism. Insulin action is manifested through binding of its monomeric form to the Insulin Receptor (IR). At present, however, our knowledge about the structural behavior of insulin is based upon inactive, multimeric, and storage-like states. The active monomeric structure, when in complex with the receptor, must be different as the residues crucial for the interactions are buried within the multimeric forms. Although the exact nature of the insulin’s induced-fit is unknown, there is strong evidence that the C-terminal part of the B-chain is a dynamic element in insulin activation and receptor binding. Here, we present the design and analysis of highly active (200–500%) insulin analogues that are truncated at residue 26 of the B-chain (B26). They show a structural convergence in the form of a new β-turn at B24-B26. We propose that the key element in insulin’s transition, from an inactive to an active state, may be the formation of the β-turn at B24-B26 associated with a trans to cis isomerisation at the B25-B26 peptide bond. Here, this turn is achieved with N-methylated L-amino acids adjacent to the trans to cis switch at the B25-B26 peptide bond or by the insertion of certain D-amino acids at B26. The resultant conformational changes unmask previously buried amino acids that are implicated in IR binding and provide structural details for new approaches in rational design of ligands effective in combating diabetes. PMID:20133841

  6. Neuroserpin Differentiates Between Forms of Tissue Type Plasminogen Activator via pH Dependent Deacylation.

    PubMed

    Carlson, Karen-Sue B; Nguyen, Lan; Schwartz, Kat; Lawrence, Daniel A; Schwartz, Bradford S

    2016-01-01

    Tissue-type plasminogen activator (t-PA), initially characterized for its critical role in fibrinolysis, also has key functions in both physiologic and pathologic processes in the CNS. Neuroserpin (NSP) is a t-PA specific serine protease inhibitor (serpin) found almost exclusively in the CNS that regulates t-PA's proteolytic activity and protects against t-PA mediated seizure propagation and blood-brain barrier disruption. This report demonstrates that NSP inhibition of t-PA varies profoundly as a function of pH within the biologically relevant pH range for the CNS, and reflects the stability, rather than the formation of NSP: t-PA acyl-enzyme complexes. Moreover, NSP differentiates between the zymogen-like single chain form (single chain t-PA, sct-PA) and the mature protease form (two chain t-PA, tct-PA) of t-PA, demonstrating different pH profiles for protease inhibition, different pH ranges over which catalytic deacylation occurs, and different pH dependent profiles of deacylation rates for each form of t-PA. NSP's pH dependent inhibition of t-PA is not accounted for by differential acylation, and is specific for the NSP-t-PA serpin-protease pair. These results demonstrate a novel mechanism for the differential regulation of the two forms of t-PA in the CNS, and suggest a potential specific regulatory role for CNS pH in controlling t-PA proteolytic activity. PMID:27378851

  7. Neuroserpin Differentiates Between Forms of Tissue Type Plasminogen Activator via pH Dependent Deacylation

    PubMed Central

    Carlson, Karen-Sue B.; Nguyen, Lan; Schwartz, Kat; Lawrence, Daniel A.; Schwartz, Bradford S.

    2016-01-01

    Tissue-type plasminogen activator (t-PA), initially characterized for its critical role in fibrinolysis, also has key functions in both physiologic and pathologic processes in the CNS. Neuroserpin (NSP) is a t-PA specific serine protease inhibitor (serpin) found almost exclusively in the CNS that regulates t-PA’s proteolytic activity and protects against t-PA mediated seizure propagation and blood–brain barrier disruption. This report demonstrates that NSP inhibition of t-PA varies profoundly as a function of pH within the biologically relevant pH range for the CNS, and reflects the stability, rather than the formation of NSP: t-PA acyl-enzyme complexes. Moreover, NSP differentiates between the zymogen-like single chain form (single chain t-PA, sct-PA) and the mature protease form (two chain t-PA, tct-PA) of t-PA, demonstrating different pH profiles for protease inhibition, different pH ranges over which catalytic deacylation occurs, and different pH dependent profiles of deacylation rates for each form of t-PA. NSP’s pH dependent inhibition of t-PA is not accounted for by differential acylation, and is specific for the NSP-t-PA serpin-protease pair. These results demonstrate a novel mechanism for the differential regulation of the two forms of t-PA in the CNS, and suggest a potential specific regulatory role for CNS pH in controlling t-PA proteolytic activity. PMID:27378851

  8. Multifunctional porous titanium oxide coating with apatite forming ability and photocatalytic activity on a titanium substrate formed by plasma electrolytic oxidation.

    PubMed

    Akatsu, T; Yamada, Y; Hoshikawa, Y; Onoki, T; Shinoda, Y; Wakai, F

    2013-12-01

    Plasma electrolytic oxidation (PEO) was used to make a multifunctional porous titanium oxide (TiO2) coating on a titanium substrate. The key finding of this study is that a highly crystalline TiO2 coating can be made by performing the PEO in an ammonium acetate (CH3COONH4) solution; the PEO coating was formed by alternating between rapid heating by spark discharges and quenching in the solution. The high crystallinity of the TiO2 led to the surface having multiple functions, including apatite forming ability and photocatalytic activity. Hydroxyapatite formed on the PEO coating when it was soaked in simulated body fluid. The good apatite forming ability can be attributed to the high density of hydroxyl groups on the anatase and rutile phases in the coating. The degradation of methylene blue under ultraviolet radiation indicated that the coating had high photocatalytic activity. PMID:24094199

  9. Special Form Testing of Sealed Source Encapsulation for High-Alpha-Activity Actinide Materials

    SciTech Connect

    Martinez, Oscar A

    2016-01-01

    In the United States all transportation of radioactive material is regulated by the U.S. Department of Transportation (DOT). Beginning in 2008 a new type of sealed-source encapsulation package was developed and tested by Oak Ridge National Laboratory (ORNL). These packages contain high-alpha-activity actinides and are regulated and transported in accordance with the requirements for DOT Class 7 hazardous material. The DOT provides specific regulations pertaining to special form encapsulation designs. The special form designation indicates that the encapsulated radioactive contents have a very low probability of dispersion even when subjected to significant structural events. The special form designs have been shown to simplify the delivery, transport, acceptance, and receipt processes. It is intended for these sealed-source encapsulations to be shipped to various facilities making it very advantageous for them to be certified as special form. To this end, DOT Certificates of Competent Authority (CoCAs) have been sought for the design suitable for containing high-alpha-activity actinide materials. This design consists of the high-alpha-activity material encapsulated within a triangular zirconia canister, referred to as a ZipCan, tile that is then enclosed by a spherical shell. The spherical shell design, with ZipCan tile inside, was tested for compliance with the special form regulations found in 49 CFR 173.469. The spherical enclosure was subjected to 9-m impact, 1 m percussion, and 10-minute thermal tests at the Packaging Evaluation Facility located at the National Transportation Research Center in Knoxville, TN USA and operated by ORNL. Before and after each test, the test units were subjected to a helium leak check and a bubble test. The ZipCan tiles and core were also subjected to the tests required for ISO 2919:2012(E), including a Class IV impact test and heat test and subsequently subjected to helium leakage rate tests [49 CFR 173.469(a)(4)(i)]. The impact

  10. Staphylococcus aureus forms spreading dendrites that have characteristics of active motility.

    PubMed

    Pollitt, Eric J G; Crusz, Shanika A; Diggle, Stephen P

    2015-01-01

    Staphylococcus aureus is historically regarded as a non-motile organism. More recently it has been shown that S. aureus can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites: branching structures that emerge from the central colony. We discovered that S. aureus can spread across the surface of media in structures that we term 'comets', which advance outwards and precede the formation of dendrites. We observed comets in a diverse selection of S. aureus isolates and they exhibit the following behaviours: (1) They consist of phenotypically distinct cores of cells that move forward and seed other S. aureus cells behind them forming a comet 'tail'; (2) they move when other cells in the comet tail have stopped moving; (3) the comet core is held together by a matrix of slime; and (4) the comets etch trails in the agar as they move forwards. Comets are not consistent with spreading motility or other forms of passive motility. Comet behaviour does share many similarities with a form of active motility known as gliding. Our observations therefore suggest that S. aureus is actively motile under certain conditions. PMID:26680153

  11. Staphylococcus aureus forms spreading dendrites that have characteristics of active motility

    PubMed Central

    Pollitt, Eric J. G.; Crusz, Shanika A.; Diggle, Stephen P.

    2015-01-01

    Staphylococcus aureus is historically regarded as a non-motile organism. More recently it has been shown that S. aureus can passively move across agar surfaces in a process called spreading. We re-analysed spreading motility using a modified assay and focused on observing the formation of dendrites: branching structures that emerge from the central colony. We discovered that S. aureus can spread across the surface of media in structures that we term ‘comets’, which advance outwards and precede the formation of dendrites. We observed comets in a diverse selection of S. aureus isolates and they exhibit the following behaviours: (1) They consist of phenotypically distinct cores of cells that move forward and seed other S. aureus cells behind them forming a comet ‘tail’; (2) they move when other cells in the comet tail have stopped moving; (3) the comet core is held together by a matrix of slime; and (4) the comets etch trails in the agar as they move forwards. Comets are not consistent with spreading motility or other forms of passive motility. Comet behaviour does share many similarities with a form of active motility known as gliding. Our observations therefore suggest that S. aureus is actively motile under certain conditions. PMID:26680153

  12. [CELLS FORM AND THEIR SENSITIVITY TO LYTIC ACTIVITY OF NATURAL KILLER CELLS UNDER THE ANTIOXIDANT ACTION].

    PubMed

    Kirpichnikova, K M; Petrov, Yu P; Filatova, N A; Gamaley, I A

    2015-01-01

    The present paper is an attempt to estimate the influence of cell surface morphology changes to functional activity under the effect of antioxidant, N-acetylcysteine (NAC), and alpha-lipoic asid (ALA). Two experimental parameters were used to characterize transformed fibroblasts 3T3-SV40 status. The functional one was the cell sensitivity to lysis by natural killer (NK) mouse splenocytes, and morphology index (cell form index) was a cell area. We showed that addition of NAC or ALA to the cell medium caused fast decrease of cell area and changes of cell form. On the other hand, their sensitivity to lysis NK cells gradually and significantly decreased. Then we compared NAC or ALA effect with the effects of other substances, which were non-antioxidants but caused cell responses which concurred with of antioxidants, at least partly. They were: latrunculin B, desorganizing actin filaments (as both antioxidants), OTZ reducing ROS level in the cell (as NAC), BSO (inhibitor of glutathione synthesis), increasing ROS level in the cell (as ALA), antibodies to gelatinases, MMP-2 and MMP-9 inactivating their activities (as both antioxidants). The results obtained showed a correlation between changes of morphology index and functional activity, sensitivity to lysis by NK cells. We suppose that geometry of cell surface might be a functional indicator of cell reaction to the antioxidant. PMID:26591569

  13. Antifungal activity and pore-forming mechanism of astacidin 1 against Candida albicans.

    PubMed

    Choi, Hyemin; Lee, Dong Gun

    2014-10-01

    In a previous report, a novel antibacterial peptide astacidin 1 (FKVQNQHGQVVKIFHH) was isolated from hemocyanin of the freshwater crayfish Pacifastacus leniusculus. In this study, the antifungal activity and mechanism of astacidin 1 were evaluated. Astacidin 1 exhibited antifungal activity against Candida albicans, Trichosporon beigelii, Malassezia furfur, and Trichophyton rubrum. Also, astacidin 1 had fungal cell selectivity in human erythrocytes without causing hemolysis. To understand the antifungal mechanism, membrane studies were done against C. albicans and T. beigelii. Flow cytometric analysis and K(+) measurement showed membrane damage, resulting in membrane permeabilization and K(+) release-induced membrane depolarization. Furthermore, the calcein leakage from liposomes mimicking C. albicans membrane demonstrated that the membrane-active action was driven by pore-forming mechanism. Live cell imaging using fluorescein isothiocyanate-labeled dextrans of various sizes suggested that the radii of pores formed in the C. albicans membrane were 1.4-2.3 nm. Therefore, the present study suggests that astacidin 1 exerts its antifungal effect by damaging the fungal membrane via pore formation. PMID:24955933

  14. Isolation of a biologically active soluble form of the hemagglutinin-neuraminidase protein of Sendai virus.

    PubMed Central

    Thompson, S D; Laver, W G; Murti, K G; Portner, A

    1988-01-01

    As a first step in establishing the three-dimensional structure of the Sendai virus hemagglutinin-neuraminidase (HN), we have isolated and characterized a potentially crystallizable form of the molecule. The sequence of HN, a surface glycoprotein, predicts a protein with an uncharged hydrophobic region near the amino terminus which is responsible for anchorage in the viral envelope. To avoid rosette formation (aggregation), which would preclude crystallization, this hydrophobic tail was removed from a membrane-free form of HN by proteolytic digestion. This digestion resulted in a single product with a molecular weight of about 10,000 less than native HN. N-terminal amino acid sequence analysis of cleaved HN (C-HN) indicated a single cleavage site at amino acid residue 131, resulting in a product consisting of the carboxyl-terminal 444 amino acids of HN. Functional analyses revealed that C-HN retained full neuraminidase activity and was able to bind erythrocytes, indicating that the N-terminal 131 residues were not necessary for these biological activities. Furthermore, this cleavage product retained the antigenic structure of intact HN, since monoclonal antibodies still bound to C-HN in enzyme-linked immunosorbent assay and Western (immuno-) blot analysis. Viewed by electron microscopy, the dimeric and tetrameric forms of intact HN form rosettes while C-HN maintains the oligomeric structure but no longer aggregates. Furthermore, the electron micrographs revealed a C-HN tetramer strikingly similar to the influenza virus neuraminidase in both size and gross structural features. Images PMID:2846877

  15. Glutathione peroxidase activity and chemical forms of selenium in tissues of rats given selenite or selenomethionine

    SciTech Connect

    Beilstein, M.A.; Whanger, P.D.

    1988-05-01

    Glutathione peroxidase (GPx) activity and deposition of selenium (Se) were examined in tissues of rats given dietary Se for 7 wk as either selenite or selenomethionine (SeMet) with 75Se radiotracer of the same chemical form. On the basis of Se:75Se ratio, all tissues of the rats fed selenite were equilibrated with the dietary source, but tissues of the SeMet fed animals maintained a ratio of Se:75Se greater than the dietary ratio. Deposition of dietary Se and 75Se was higher in most tissues of rats fed SeMet. Muscle 75Se was the largest single tissue pool of 75Se in both groups accounting for one-third of recovered 75Se in the rats fed selenite, and one-half of recovered 75Se in the rats fed SeMet. Tissue GPx activities were not different between the two dietary groups. The proportion of Se as GPx in tissues was highest in erythrocytes of the rats fed selenite (.81) and lowest in testes and epididymides of the rats fed SeMet (.009). The proportion of Se present in cytosolic GPx was consistently higher in tissues of rats fed selenite. Erythrocytes of the rats fed SeMet had more 75Se associated with hemoglobin, and muscle cytosols of the rats fed selenite had more 75Se associated with the G-protein. The proportion of 75Se as SeMet determined by ion exchange chromatography of tissue hydrolysates was higher in tissues of rats fed SeMet (highest in muscle and hemoglobin, 70%, and lowest in testes, 16%). In contrast, selenocysteine was the predominant form of Se present in tissues of rats given selenite. These results indicate that the form of Se administered will influence the form in the tissues, the percentage of Se with GPx and the body burden of Se.

  16. 18 CFR 141.1 - FERC Form No. 1, Annual report of Major electric utilities, licensees and others.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., throughout the United States and its possessions, having sales or transmission service equal to Major as... agency, authority or instrumentality of the United States, nor is it prescribed for municipalities as defined in section 3 of the Federal Power Act; (i.e., a city, county, irrigation district,...

  17. 18 CFR 131.80 - FERC Form No. 556, Certification of qualifying facility status for an existing or a proposed...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Certification of qualifying facility status for an existing or a proposed small power production or cogeneration... or a proposed small power production or cogeneration facility. Link to an amendment published at 75... ____ Certification of Qualifying Facility Status for an Existing or a Proposed Small Power Production or...

  18. Activation of the hole-forming toxin aerolysin by extracellular processing.

    PubMed Central

    Howard, S P; Buckley, J T

    1985-01-01

    A precursor-product relationship between aerolysin and a protein with a higher molecular weight was observed in culture supernatants of Aeromonas hydrophila. The larger protein was isolated by ammonium sulfate precipitation and ion-exchange and hydroxyapatite chromatography and compared with purified aerolysin. It was at least 250 times less hemolytic than aerolysin. Both proteins had the same amino acid sequence at the amino terminus. Cyanogen bromide fragments obtained from the two were identical except that each protein contained one unique fragment, and the fragment from the larger protein was 2,500 daltons larger than the fragment obtained from aerolysin. Treatment with trypsin or with an extracellular Aeromonas protease resulted in rapid conversion of the larger protein to a form corresponding in molecular weight and activity to aerolysin. The results indicate that aerolysin is exported to the culture supernatant as a protoxin which is later activated by proteolytic removal of a peptide from the C terminus. Images PMID:3891735

  19. [Study of the Sporothrix schenkii (yeast forms) extract. Electrophoretic and immunoelectrophoretic analyses: characterization of enzymatic activities].

    PubMed

    Walbaum, S; Duriez, T; Dujardin, L; Biguet, J

    1978-07-28

    An extract from living yeast forms of S. schenckii was prepared. The yeasts originated from a shake culture in B.H.I. broth (Difco) incubated for 3 days at 35 degrees C in darkness; they were harvested, washed and disrupted with glass beads in a model MSK Braun mechanical cell homogenizer; a freezing-thawing was added to improve the extract. After electrophoretic separation in agarose gel, the extract's components were characterized by their enzymic activity; with this technique, 30 bands were revealed. These enzymic activities were also investigated on the antigenic fractions of the extract revealed by a rabbit hyperimmunserum: 16 among 22 immunoprecipitates are identified by their catalytic properties. Study of the earliest precipitating antibodies (appearing-order and enzymic caracterization) in rabbits just immunized completes this work. How to ameliorate the quality of the extract by culture and extraction conditions is also specified. PMID:692628

  20. Role of activation in alveolar macrophage-mediated suppression of the plaque-forming cell response.

    PubMed Central

    Mbawuike, I N; Herscowitz, H B

    1988-01-01

    Alveolar macrophages (AM) are highly suppressive of the in vitro plaque-forming cell (PFC) response of spleen cells obtained from mice primed with sheep erythrocytes. Comparison of macrophage populations obtained from disparate anatomical sites revealed that although in both cases there was a cell-concentration-dependent suppression of the PFC response, resident AM or AM activated as a result of intravenous injection of Mycobacterium bovis BCG were equally suppressive at the doses examined. Although there was a similar dose-dependent suppression with peritoneal macrophages, BCG-activated cells were more suppressive of the PFC response than were resident cells. In contrast, splenic macrophages at comparable concentrations were not at all suppressive. Resident AM exhibited significantly lower levels of 5'-nucleotidase activity than did resident peritoneal macrophages. Macrophage-mediated suppression of the in vitro PFC response could not be attributed to the release of toxic oxygen metabolites (H2O2, O2- ,and .OH) or prostaglandins, since the addition of catalase, superoxide dismutase, 2-mercaptoethanol, or indomethacin did not completely reverse suppression. These results suggest that the lung microenvironment may maintain AM in an activated state which contributes to their potential immunoregulatory functions. PMID:2830191

  1. Theoretical study of the phototoxicity of naproxen and the active form of nabumetone.

    PubMed

    Musa, Klefah A K; Eriksson, Leif A

    2008-10-30

    Density functional theory using the hybrid functional B3LYP has been employed in order to study the mechanisms of photoinduced decomposition of the closely related nonsteroidal anti-inflammatory drugs naproxen (NP) and 6-methoxy-2-naphthylacetic acid (MNAA; the active form of nabumetone). The photochemical properties and computed energies of various species obtained in this study show that both drugs dominate in their deprotonated forms at physiological pH. The deprotonated acids are unable to decarboxylate from their excited singlets; instead, they decarboxylate from their first excited triplet states with high efficiency, overcoming energy barriers less than 3 and 1 kcal/mol for MNAA and NP, respectively. The ultraviolet and visible spectra of the neutral, deprotonated, and decarboxylated moieties of MNAA and NP are more-or-less similar but with higher probabilites (oscillator strength) for the latter. This fact, as well as the higher reactivity of NP, is explained in terms of the electron-donating effect of the additional methyl group present in NP. Singlet oxygen, superoxide radical anion, and corresponding peroxyl radical species are expected to be formed in different steps throughout the proposed photodegradation pathways of both drugs, which give rise to their effects on biomolecules, for example, lipid peroxidation. PMID:18834087

  2. Pore-forming activity and structural autoinhibition of the gasdermin family.

    PubMed

    Ding, Jingjin; Wang, Kun; Liu, Wang; She, Yang; Sun, Qi; Shi, Jianjin; Sun, Hanzi; Wang, Da-Cheng; Shao, Feng

    2016-07-01

    Inflammatory caspases cleave the gasdermin D (GSDMD) protein to trigger pyroptosis, a lytic form of cell death that is crucial for immune defences and diseases. GSDMD contains a functionally important gasdermin-N domain that is shared in the gasdermin family. The functional mechanism of action of gasdermin proteins is unknown. Here we show that the gasdermin-N domains of the gasdermin proteins GSDMD, GSDMA3 and GSDMA can bind membrane lipids, phosphoinositides and cardiolipin, and exhibit membrane-disrupting cytotoxicity in mammalian cells and artificially transformed bacteria. Gasdermin-N moved to the plasma membrane during pyroptosis. Purified gasdermin-N efficiently lysed phosphoinositide/cardiolipin-containing liposomes and formed pores on membranes made of artificial or natural phospholipid mixtures. Most gasdermin pores had an inner diameter of 10–14 nm and contained 16 symmetric protomers. The crystal structure of GSDMA3 showed an autoinhibited two-domain architecture that is conserved in the gasdermin family. Structure-guided mutagenesis demonstrated that the liposome-leakage and pore-forming activities of the gasdermin-N domain are required for pyroptosis. These findings reveal the mechanism for pyroptosis and provide insights into the roles of the gasdermin family in necrosis, immunity and diseases. PMID:27281216

  3. Trypanocidal activity of the essential oils in their conventional and nanoemulsion forms: in vitro tests.

    PubMed

    Baldissera, Matheus D; Da Silva, Aleksandro S; Oliveira, Camila B; Zimmermann, Carine E P; Vaucher, Rodrigo A; Santos, Roberto C V; Rech, Virginia C; Tonin, Alexandre A; Giongo, Janice L; Mattos, Cristiane B; Koester, Letícia; Santurio, Janio M; Monteiro, Silvia G

    2013-07-01

    The aim of this study was to investigate the susceptibility in vitro of Trypanosoma evansi to the essential oils of andiroba (Carapa guaianensis) and aroeira (Schinus molle), in their conventional and nanostructured forms. For that, pure oils at concentrations of 0.5%, 1.0% and 2.0% were used. A negative control (untreated) and a positive control (diminazene aceturate 0.5%) were used as comparative parameters. Later, the same tests were performed, using nanoemulsions oils at concentrations of 0.5% and 1.0%. The tests were carried out in triplicates and the numbers of parasites were quantified on 1, 3 and 6 h from onset of the study. A dose-dependent reduction in the number of parasites to the forms of two oils tested was observed after 1 h. The concentration of parasites was significantly reduced at low concentrations after 3 h, as well as at 6 h no alive parasites were observed for the essential oils tested. Ours findings indicate, for the first time, that oils of andiroba and aroeira (in their conventional and nanoemulsion forms) have high activity against T. evansi in vitro, leading to the suggestion that these oils may be applied as an alternative treatment for this disease. PMID:23562884

  4. The TCP1γ subunit of Leishmania donovani forms a biologically active homo-oligomeric complex.

    PubMed

    Bhaskar; Mitra, Kalyan; Kuldeep, Jitendra; Siddiqi, Mohammad Imran; Goyal, Neena

    2015-12-01

    Chaperonins are a class of molecular chaperons that encapsulate nascent or stress-denatured proteins and assist their intracellular assembly and folding in an ATP-dependent manner. The ubiquitous eukaryotic chaperonin, TCP1 ring complex is a hetero-oligomeric complex comprising two rings, each formed of eight subunits that may have distinct substrate recognition and ATP hydrolysis properties. In Leishmania, only the TCP1γ subunit has been cloned and characterized. It exhibited differential expression at various growth stages of promastigotes. In the present study, we expressed the TCP1γ subunit in Escherichia coli to investigate whether it forms chaperonin-like complexes and plays a role in protein folding. LdTCP1γ formed high-molecular-weight complexes within E. coli cells as well as in Leishmania cell lysates. The recombinant protein is arranged into two back-to-back rings of seven subunits each, as predicted by homology modelling and observed by negative staining electron microscopy. This morphology is consistent with that of the oligomeric double-ring group I chaperonins found in mitochondria. The LdTCP1γ homo-oligomeric complex hydrolysed ATP, and was active as assayed by luciferase refolding. Thus, the homo-oligomer performs chaperonin reactions without partner subunit(s). Further, co-immunoprecipitation studies revealed that LdTCP1γ interacts with actin and tubulin proteins, suggesting that the complex may have a role in maintaining the structural dynamics of the cytoskeleton of parasites. PMID:26395202

  5. Dynamin Forms a Src Kinase–sensitive Complex with Cbl and Regulates Podosomes and Osteoclast Activity

    PubMed Central

    Bruzzaniti, Angela; Neff, Lynn; Sanjay, Archana; Horne, William C.; De Camilli, Pietro; Baron, Roland

    2005-01-01

    Podosomes are highly dynamic actin-containing adhesion structures found in osteoclasts, macrophages, and Rous sarcoma virus (RSV)-transformed fibroblasts. After integrin engagement, Pyk2 recruits Src and the adaptor protein Cbl, forming a molecular signaling complex that is critical for cell migration, and deletion of any molecule in this complex disrupts podosome ring formation and/or decreases osteoclast migration. Dynamin, a GTPase essential for endocytosis, is also involved in actin cytoskeleton remodeling and is localized to podosomes where it has a role in actin turnover. We found that dynamin colocalizes with Cbl in the actin-rich podosome belt of osteoclasts and that dynamin forms a complex with Cbl in osteoclasts and when overexpressed in 293VnR or SYF cells. The association of dynamin with Cbl in osteoclasts was decreased by Src tyrosine kinase activity and we found that destabilization of the dynamin-Cbl complex involves the recruitment of Src through the proline-rich domain of Cbl. Overexpression of dynamin increased osteoclast bone resorbing activity and migration, whereas overexpression of dynK44A decreased osteoclast resorption and migration. These studies suggest that dynamin, Cbl, and Src coordinately participate in signaling complexes that are important in the assembly and remodeling of the actin cytoskeleton, leading to changes in osteoclast adhesion, migration, and resorption. PMID:15872089

  6. Dynamin forms a Src kinase-sensitive complex with Cbl and regulates podosomes and osteoclast activity.

    PubMed

    Bruzzaniti, Angela; Neff, Lynn; Sanjay, Archana; Horne, William C; De Camilli, Pietro; Baron, Roland

    2005-07-01

    Podosomes are highly dynamic actin-containing adhesion structures found in osteoclasts, macrophages, and Rous sarcoma virus (RSV)-transformed fibroblasts. After integrin engagement, Pyk2 recruits Src and the adaptor protein Cbl, forming a molecular signaling complex that is critical for cell migration, and deletion of any molecule in this complex disrupts podosome ring formation and/or decreases osteoclast migration. Dynamin, a GTPase essential for endocytosis, is also involved in actin cytoskeleton remodeling and is localized to podosomes where it has a role in actin turnover. We found that dynamin colocalizes with Cbl in the actin-rich podosome belt of osteoclasts and that dynamin forms a complex with Cbl in osteoclasts and when overexpressed in 293VnR or SYF cells. The association of dynamin with Cbl in osteoclasts was decreased by Src tyrosine kinase activity and we found that destabilization of the dynamin-Cbl complex involves the recruitment of Src through the proline-rich domain of Cbl. Overexpression of dynamin increased osteoclast bone resorbing activity and migration, whereas overexpression of dynK44A decreased osteoclast resorption and migration. These studies suggest that dynamin, Cbl, and Src coordinately participate in signaling complexes that are important in the assembly and remodeling of the actin cytoskeleton, leading to changes in osteoclast adhesion, migration, and resorption. PMID:15872089

  7. A transcriptionally active form of TFIIIC is modified in poliovirus-infected HeLa cells.

    PubMed Central

    Clark, M E; Dasgupta, A

    1990-01-01

    In HeLa cells, RNA polymerase III (pol III)-mediated transcription is severely inhibited by poliovirus infection. This inhibition is due primarily to the reduction in transcriptional activity of the pol III transcription factor TFIIIC in poliovirus-infected cells. However, the specific binding of TFIIIC to the VAI gene B-box sequence, as assayed by DNase I footprinting, is not altered by poliovirus infection. We have used gel retardation analysis to analyze TFIIIC-DNA complexes formed in nuclear extracts prepared from mock- and poliovirus-infected cells. In mock-infected cell extracts, two closely migrating TFIIIC-containing complexes, complexes I and II, were detected in the gel retardation assay. The slower migrating complex, complex I, was absent in poliovirus-infected cell extracts, and an increase occurred in the intensity of the faster-migrating complex (complex II). Also, in poliovirus-infected cell extracts, a new, rapidly migrating complex, complex III, was formed. Complex III may have been the result of limited proteolysis of complex I or II. These changes in TFIIIC-containing complexes in poliovirus-infected cell extracts correlated kinetically with the decrease in TFIIIC transcriptional activity. Complexes I, II, and III were chromatographically separated; only complex I was transcriptionally active and specifically restored pol III transcription when added to poliovirus-infected cell extracts. Acid phosphatase treatment partially converted complex I to complex II but did not affect the binding of complex II or III. Dephosphorylation and limited proteolysis of TFIIIC are discussed as possible mechanisms for the inhibition of pol III-mediated transcription by poliovirus. Images PMID:2204807

  8. Extensive Post-translational Modification of Active and Inactivated Forms of Endogenous p53*

    PubMed Central

    DeHart, Caroline J.; Chahal, Jasdave S.; Flint, S. J.; Perlman, David H.

    2014-01-01

    The p53 tumor suppressor protein accumulates to very high concentrations in normal human fibroblasts infected by adenovirus type 5 mutants that cannot direct assembly of the viral E1B 55-kDa protein-containing E3 ubiquitin ligase that targets p53 for degradation. Despite high concentrations of nuclear p53, the p53 transcriptional program is not induced in these infected cells. We exploited this system to examine select post-translational modifications (PTMs) present on a transcriptionally inert population of endogenous human p53, as well as on p53 activated in response to etoposide treatment of normal human fibroblasts. These forms of p53 were purified from whole cell lysates by means of immunoaffinity chromatography and SDS-PAGE, and peptides derived from them were subjected to nano-ultra-high-performance LC-MS and MS/MS analyses on a high-resolution accurate-mass MS platform (data available via ProteomeXchange, PXD000464). We identified an unexpectedly large number of PTMs, comprising phosphorylation of Ser and Thr residues, methylation of Arg residues, and acetylation, ubiquitinylation, and methylation of Lys residues—for example, some 150 previously undescribed modifications of p53 isolated from infected cells. These modifications were distributed across all functional domains of both forms of the endogenous human p53 protein, as well as those of an orthologous population of p53 isolated from COS-1 cells. Despite the differences in activity, including greater in vitro sequence-specific DNA binding activity exhibited by p53 isolated from etoposide-treated cells, few differences were observed in the location, nature, or relative frequencies of PTMs on the two populations of human p53. Indeed, the wealth of PTMs that we have identified is consistent with a far greater degree of complex, combinatorial regulation of p53 by PTM than previously anticipated. PMID:24056736

  9. FUNCTION FOLLOWS FORM: ACTIVATION OF SHAPE & FUNCTION FEATURES DURING OBJECT IDENTIFICATION

    PubMed Central

    Yee, Eiling; Huffstetler, Stacy; Thompson-Schill, Sharon L.

    2011-01-01

    Most theories of semantic memory characterize knowledge of a given object as comprising a set of semantic features. But how does conceptual activation of these features proceed during object identification? We present the results of a pair of experiments that demonstrate that object recognition is a dynamically unfolding process in which function follows form. We used eye movements to explore whether activating one object’s concept leads to the activation of others that share perceptual (shape) or abstract (function) features. Participants viewed four-picture displays and clicked on the picture corresponding to a heard word. In critical trials, the conceptual representation of one of the objects in the display was similar in shape or function (i.e., its purpose) to the heard word. Importantly, this similarity was not apparent in the visual depictions (e.g., for the target “frisbee,” the shape-related object was a triangular slice of pizza – a shape that a frisbee cannot take); preferential fixations on the related object were therefore attributable to overlap of the conceptual representations on the relevant features. We observed relatedness effects for both shape and function, but shape effects occurred earlier than function effects. We discuss the implications of these findings for current accounts of the representation of semantic memory. PMID:21417543

  10. Bactericidal Activity of N-Chlorotaurine against Biofilm-Forming Bacteria Grown on Metal Disks

    PubMed Central

    Ammann, Christoph G.; Fille, Manfred; Hausdorfer, Johann; Nogler, Michael

    2014-01-01

    Many orthopedic surgeons consider surgical irrigation and debridement with prosthesis retention as a treatment option for postoperative infections. Usually, saline solution with no added antimicrobial agent is used for irrigation. We investigated the activity of N-chlorotaurine (NCT) against various biofilm-forming bacteria in vitro and thereby gained significant information on its usability as a soluble and well-tolerated active chlorine compound in orthopedic surgery. Biofilms of Staphylococcus aureus were grown on metal alloy disks and in polystyrene dishes for 48 h. Subsequently, they were incubated for 15 min to 7 h in buffered solutions containing therapeutically applicable concentrations of NCT (1%, 0.5%, and 0.1%; 5.5 to 55 mM) at 37°C. NCT inactivated the biofilm in a time- and dose-dependent manner. Scanning electron microscopy revealed disturbance of the biofilm architecture by rupture of the extracellular matrix. Assays with reduction of carboxanilide (XTT) showed inhibition of the metabolism of the bacteria in biofilms. Quantitative cultures confirmed killing of S. aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa biofilms on metal alloy disks by NCT. Clinical isolates were slightly more resistant than ATCC type strains, but counts of CFU were reduced at least 10-fold by 1% NCT within 15 min in all cases. NCT showed microbicidal activity against various bacterial strains in biofilms. Whether this can be transferred to the clinical situation should be the aim of future studies. PMID:24492358

  11. Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.

    PubMed

    Liu, Xing; Zhang, Zhibin; Ruan, Jianbin; Pan, Youdong; Magupalli, Venkat Giri; Wu, Hao; Lieberman, Judy

    2016-07-01

    Inflammatory caspases (caspases 1, 4, 5 and 11) are activated in response to microbial infection and danger signals. When activated, they cleave mouse and human gasdermin D (GSDMD) after Asp276 and Asp275, respectively, to generate an N-terminal cleavage product (GSDMD-NT) that triggers inflammatory death (pyroptosis) and release of inflammatory cytokines such as interleukin-1β. Cleavage removes the C-terminal fragment (GSDMD-CT), which is thought to fold back on GSDMD-NT to inhibit its activation. However, how GSDMD-NT causes cell death is unknown. Here we show that GSDMD-NT oligomerizes in membranes to form pores that are visible by electron microscopy. GSDMD-NT binds to phosphatidylinositol phosphates and phosphatidylserine (restricted to the cell membrane inner leaflet) and cardiolipin (present in the inner and outer leaflets of bacterial membranes). Mutation of four evolutionarily conserved basic residues blocks GSDMD-NT oligomerization, membrane binding, pore formation and pyroptosis. Because of its lipid-binding preferences, GSDMD-NT kills from within the cell, but does not harm neighbouring mammalian cells when it is released during pyroptosis. GSDMD-NT also kills cell-free bacteria in vitro and may have a direct bactericidal effect within the cytosol of host cells, but the importance of direct bacterial killing in controlling in vivo infection remains to be determined. PMID:27383986

  12. In Vitro Antimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm Forming Staphylococcus epidermidis Strains

    PubMed Central

    Wojtyczka, Robert D.; Kępa, Małgorzata; Idzik, Danuta; Kabała-Dzik, Agata; Wąsik, Tomasz J.

    2013-01-01

    The aim of the presented study was to examine the antimicrobial activity of ethanol extract of Polish propolis (EEPP) against biofilm-forming CoNS strains in vitro. Our results revealed that EEPP displayed varying degrees of activity against CoNS with MIC values ranging from 1.56 to 0.78 mg/mL. The average MIC was 1.13 ± 0.39 mg/mL while calculated MIC50 and MIC90 values were 0.78 mg/mL and 1.56 mg/mL, respectively. The biofilm formation ability by all tested S. epidermidis strains was inhibited at EEPP concentrations ranging from 0.39 to 1.56 mg/mL. The degree of reduction of AlamarBlue was directly associated with the proliferation of S. epidermidis strains. The increased proliferation of S. epidermidis strains was observed after 12 and 24 hours of incubation in the presence of EEPP concentrations ranging from 0.025 to 0.39 mg/mL. These results suggest that antimicrobial activities of EEPP against S. epidermidis expressed as the reduction of bacterial growth, reduction of biofilm formation ability, and the intensity of proliferation were significantly affected by incubation time and EEPP concentration used as well as the interactions between these factors. PMID:23662143

  13. [Relationship between the oxidation-reduction system of astrocytes with production of active forms of oxygen].

    PubMed

    Semenkova, G N; Kvacheva, Z B; Obydennikova, S V; Cherenkevich, S N; Titov, L P

    1998-01-01

    Cells of neuroglia--the astrocytes are of interest from the point of view of their participation in phagocytosis. Phagocyte ability to generate active oxygen forms (AOF) as used as the basic criterion of the estimation of their functional activity. For the purpose to clear up molecular and cellular mechanisms of phagocytosis a study of astrocyte redox-systems, participating in production of AOF, was undertaken. Registration of AOF in astrocytes was carried out using a method of luminol-dependent chemiluminescence. Primary culture of guinea pig astrocytes was used. Spontaneous chemiluminescence of low intensity was found for the astrocytes at the presence of luminol. The destruction of the cells was accompanied by a significant growth of the intensity of spontaneous chemiluminescence. Suspension of endocutosis inductors, particle of latex and phytohemagglutinin, added to astrocytes did not result in formation of AOF, characteristic for other cells, possessing phagocytosis. It was established, that addition of hydrogen peroxide destroys astrocytes at the presence of luminol and gives rise to the emission. Chemiluminescence was not observed in similar experiments with intact cells. A conclusion was made that inside astrocytes there are structures, which show peroxidase-like activity. PMID:9848214

  14. Suppression of human immunodeficiency virus type 1 activity in vitro by oligonucleotides which form intramolecular tetrads.

    PubMed

    Rando, R F; Ojwang, J; Elbaggari, A; Reyes, G R; Tinder, R; McGrath, M S; Hogan, M E

    1995-01-27

    An oligonucleotide (I100-15) composed of only deoxyguanosine and thymidine was able to inhibit human immunodeficiency virus type-1 (HIV-1) in culture assay systems. I100-15 did not block virus entry into cells but did reduce viral-specific transcripts. As assessed by NMR and polyacrylamide gel methods, I100-15 appears to form a structure in which two stacked guanosine tetrads are connected by three two-base long loops. Structure/activity experiments indicated that formation of intramolecular guanosine tetrads was necessary to achieve maximum antiviral activity. The single deoxyguanosine nucleotide present in each loop was found to be extremely important for the overall antiviral activity. The toxicity of I100-15 was determined to be well above the 50% effective dose (ED50) in culture which yielded a high therapeutic index (> 100). The addition of a cholesterol moiety to the 3' terminus of I100-15 (I100-23) reduced the ED50 value to less than 50 nM (from 0.12 microM for I100-15) and increased the duration of viral suppression to greater than 21 days (versus 7-10 days for I100-15) after removal of the drug from infected cell cultures. The favorable therapeutic index of such molecules coupled with the prolonged suppression of HIV-1, suggest that such compounds further warrant investigation as potential therapeutic agents. PMID:7829511

  15. 77 FR 9842 - Revisions to Form, Procedures and Criteria for Certification of Qualifying Facility Status for a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-21

    ... Register of Tuesday, March 30, 2010 (75 FR 15950). The final rule document adopted revisions to FERC Form... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission 18 CFR Part 292 Revisions to Form, Procedures and Criteria for...

  16. Correlation of primate red nucleus discharge with muscle activity during free-form arm movements.

    PubMed

    Miller, L E; van Kan, P L; Sinkjaer, T; Andersen, T; Harris, G D; Houk, J C

    1993-09-01

    1. We recorded from 239 neurons located in the magnocellular division of the red nucleus of four alert macaque monkeys. At the same time, we recorded electromyographic (EMG) signals from as many as twenty electrodes chronically implanted on muscles of the shoulder, arm, forearm and hand. We recorded EMG signals for periods ranging from several months to a year. 2. The monkeys were trained to perform three free-form food retrieval tasks, each of which activated all of the recorded muscles and most of the neurons. The 'prehension' task required simply that the monkey grasp a piece of food from a fixed point in space. The 'barrier' task required the monkey to reach around a small barrier to obtain the food, and the 'Kluver' task required that food be removed from small holes. During the prehension task, we found approximately equal numbers of neurons that were strongly active while the hand was being moved toward the target (70% of units), and while the food was being grasped (60%). Relatively few units were active as the hand was returned to the mouth (15%). 3. Data files of 1-2 min duration were collected while the monkey performed a single behavioural task. Whenever possible, we recorded files for all three tasks from each neuron. For each file we calculated long time-span analog cross-correlations (+/- 1.28 s) between instantaneous neuronal firing rate and each of the full-wave rectified, low-pass filtered EMG signals. We used the peak correlation and the time of the peak as two summary measures of the functional relation between modulation of neuronal activity and EMG. 4. The magnitude of the strongest correlations was between 0.4 and 0.5 (normalized to a perfect correlation of +/- 1.0). Distal muscles were the most frequently correlated, and extensors were more frequently correlated than flexors. For all monkeys, the lags for well correlated muscles were distributed broadly about a uni-modal value near 0 ms. Eighty five per cent of the correlations larger than

  17. ESR dating of barite in sulphide deposits formed by the sea-floor hydrothermal activities.

    PubMed

    Toyoda, Shin; Fujiwara, Taisei; Uchida, Ai; Ishibashi, Jun-ichiro; Nakai, Shun'ichi; Takamasa, Asako

    2014-06-01

    Barite is a mineral newly found to be practically useful for electron spin resonance (ESR) dating of sulphide deposits formed by the sea-floor hydrothermal activities. The recent studies for the properties of the ESR dating signal in barite are summarised in the present paper as well as the formulas for corrections for accurate dose-rate estimation are developed including the dose-rate conversion factors, shape correction for gamma-ray dose and decay of (226)Ra. Although development of the techniques for ESR dating of barite has been completed, further comparative studies with other dating techniques such as U-Th and (226)Ra-(210)Pb dating are necessary for the technique to be widely used. PMID:24795384

  18. Identification of Triclosan-O-Sulfate and other transformation products of Triclosan formed by activated sludge.

    PubMed

    Chen, Xijuan; Casas, Mònica Escolà; Nielsen, Jeppe Lund; Wimmer, Reinhard; Bester, Kai

    2015-02-01

    Aerobic degradation experiments of Triclosan were performed in activated sludge to identify possible transformation products for this compound. During 7 days, the formation of biotransformation products such as 2,4-Dichlorophenol, 4-Chlorocatechol, 5-Hydroxy-Triclosan and other Monohydroxy-Triclosan derivatives as well as Dihydroxy-Triclosan-derivatives were observed. The structure of 5-Hydroxy-Triclosan was elucidated by NMR data for the first time in sludge degradation experiments. Additionally the production of a hitherto unknown transformation product in sludge, i.e., Triclosan-O-Sulfate was detected. During the incubations, the concentrations of this transformation product changed from zero to 330 μg L(-1). Based on the analysis of the biodegradation products, three types of reactions were identified: 1) chemical scission of ether bond to form phenols and catechols, 2) addition of OH moieties to the aromatic ring, and 3) adding of methyl or sulfate groups to the original hydroxyl group. PMID:25306094

  19. Hydrolytic and autolytic behavior of two forms of calcium-activated neutral protease (CANP).

    PubMed

    Inomata, M; Hayashi, M; Nakamura, M; Imahori, K; Kawashima, S

    1985-08-01

    Some endogenous substrates were incubated with two forms of calcium-activated neutral protease (CANP) with high (muCANP) and low (mCANP) sensitivities to calcium ions. In addition to analyses of the processes of their degradation, changes in the molecular properties of these CANPs were also examined. Among the tested substrate proteins, the myosin heavy chain of rabbit skeletal muscle myofibrils and spectrin or band 3 protein of human erythrocyte membranes were degraded relatively rapidly. So far as these proteins were concerned, a higher degradation velocity was observed for muCANP than for mCANP. Vimentin from ascites tumor cells was degraded most rapidly and no difference was observed in degradation velocity between muCANP and mCANP. In all cases, muCANP and mCANP produced different proteolytic peptide fragments, suggesting the different substrate-specificities of these CANPs. The degradation of substrates always accompanied the autodigestion of CANPs, and the small subunits of both CANPs were degraded in the early stage of the autodigestion. The large subunit of muCANP (79K) was converted to a 76K polypeptide via a 77K polypeptide as an intermediate. The autodigested muCANP with 76K polypeptide retained sufficient protease activity and, moreover, its calcium-sensitivity was higher than that of intact muCANP. The possibility is thus proposed that restricted autodigestion is a necessary activation step for the appearance of activity of muCANP. No such transition was observed for mCANP. PMID:2999095

  20. Patterns of regional brain activation associated with different forms of motor learning.

    PubMed

    Ghilardi, M; Ghez, C; Dhawan, V; Moeller, J; Mentis, M; Nakamura, T; Antonini, A; Eidelberg, D

    2000-07-14

    To examine the variations in regional cerebral blood flow during execution and learning of reaching movements, we employed a family of kinematically and dynamically controlled motor tasks in which cognitive, mnemonic and executive features of performance were differentiated and characterized quantitatively. During 15O-labeled water positron emission tomography (PET) scans, twelve right-handed subjects moved their dominant hand on a digitizing tablet from a central location to equidistant targets displayed with a cursor on a computer screen in synchrony with a tone. In the preceding week, all subjects practiced three motor tasks: 1) movements to a predictable sequence of targets; 2) learning of new visuomotor transformations in which screen cursor motion was rotated by 30 degrees -60 degrees; 3) learning new target sequences by trial and error, by using previously acquired routines in a task placing heavy load on spatial working memory. The control condition was observing screen and audio displays. Subtraction images were analyzed with Statistical Parametric Mapping to identify significant brain activation foci. Execution of predictable sequences was characterized by a modest decrease in movement time and spatial error. The underlying pattern of activation involved primary motor and sensory areas, cerebellum, basal ganglia. Adaptation to a rotated reference frame, a form of procedural learning, was associated with decrease in the imposed directional bias. This task was associated with activation in the right posterior parietal cortex. New sequences were learned explicitly. Significant activation was found in dorsolateral prefrontal and anterior cingulate cortices. In this study, we have introduced a series of flexible motor tasks with similar kinematic characteristics and different spatial attributes. These tasks can be used to assess specific aspects of motor learning with imaging in health and disease. PMID:10882792

  1. The Role of Biofilms in the Sedimentology of Actively Forming Gypsum Deposits at Guerrero Negro, Mexico

    NASA Astrophysics Data System (ADS)

    Vogel, Marilyn B.; Des Marais, David J.; Turk, Kendra A.; Parenteau, Mary N.; Jahnke, Linda L.; Kubo, Michael D. Y.

    2009-11-01

    Actively forming gypsum deposits at the Guerrero Negro sabkha and saltern system provided habitats for stratified, pigmented microbial communities that exhibited significant morphological and phylogenetic diversity. These deposits ranged from meter-thick gypsum crusts forming in saltern seawater concentration ponds to columnar microbial mats with internally crystallized gypsum granules developing in natural anchialine pools. Gypsum-depositing environments were categorized as forming precipitation surfaces, biofilm-supported surfaces, and clastic surfaces. Each surface type was described in terms of depositional environment, microbial diversity, mineralogy, and sedimentary fabrics. Precipitation surfaces developed in high-salinity subaqueous environments where rates of precipitation outpaced the accumulation of clastic, organic, and/or biofilm layers. These surfaces hosted endolithic biofilms comprised predominantly of oxygenic and anoxygenic phototrophs, sulfate-reducing bacteria, and bacteria from the phylum Bacteroidetes. Biofilm-supported deposits developed in lower-salinity subaqueous environments where light and low water-column turbulence supported dense benthic microbial communities comprised mainly of oxygenic phototrophs. In these settings, gypsum granules precipitated in the extracellular polymeric substance (EPS) matrix as individual granules exhibiting distinctive morphologies. Clastic surfaces developed in sabkha mudflats that included gypsum, carbonate, and siliclastic particles with thin gypsum/biofilm components. Clastic surfaces were influenced by subsurface brine sheets and capillary evaporation and precipitated subsedimentary gypsum discs in deeper regions. Biofilms appeared to influence both chemical and physical sedimentary processes in the various subaqueous and subaerially exposed environments studied. Biofilm interaction with chemical sedimentary processes included dissolution and granularization of precipitation surfaces, formation of

  2. The role of biofilms in the sedimentology of actively forming gypsum deposits at Guerrero Negro, Mexico.

    PubMed

    Vogel, Marilyn B; Des Marais, David J; Turk, Kendra A; Parenteau, Mary N; Jahnke, Linda L; Kubo, Michael D Y

    2009-11-01

    Actively forming gypsum deposits at the Guerrero Negro sabkha and saltern system provided habitats for stratified, pigmented microbial communities that exhibited significant morphological and phylogenetic diversity. These deposits ranged from meter-thick gypsum crusts forming in saltern seawater concentration ponds to columnar microbial mats with internally crystallized gypsum granules developing in natural anchialine pools. Gypsum-depositing environments were categorized as forming precipitation surfaces, biofilm-supported surfaces, and clastic surfaces. Each surface type was described in terms of depositional environment, microbial diversity, mineralogy, and sedimentary fabrics. Precipitation surfaces developed in high-salinity subaqueous environments where rates of precipitation outpaced the accumulation of clastic, organic, and/or biofilm layers. These surfaces hosted endolithic biofilms comprised predominantly of oxygenic and anoxygenic phototrophs, sulfate-reducing bacteria, and bacteria from the phylum Bacteroidetes. Biofilm-supported deposits developed in lower-salinity subaqueous environments where light and low water-column turbulence supported dense benthic microbial communities comprised mainly of oxygenic phototrophs. In these settings, gypsum granules precipitated in the extracellular polymeric substance (EPS) matrix as individual granules exhibiting distinctive morphologies. Clastic surfaces developed in sabkha mudflats that included gypsum, carbonate, and siliclastic particles with thin gypsum/biofilm components. Clastic surfaces were influenced by subsurface brine sheets and capillary evaporation and precipitated subsedimentary gypsum discs in deeper regions. Biofilms appeared to influence both chemical and physical sedimentary processes in the various subaqueous and subaerially exposed environments studied. Biofilm interaction with chemical sedimentary processes included dissolution and granularization of precipitation surfaces, formation of

  3. Electrically active centers formed in silicon during the high-temperature diffusion of boron and aluminum

    SciTech Connect

    Sobolev, N. A.; Loshachenko, A. S.; Poloskin, D. S.; Shek, E. I.

    2013-02-15

    The parameters of electrically active centers formed during the high-temperature diffusion of boron and aluminum into silicon in various media are studied by the Hall method and capacitance spectroscopy. It is found that the variation in the resistivity of the n base of the structures with p-n junctions fabricated in the study is controlled by the formation of three donor levels Q1, E4, and Q3 with the energies E{sub c} - 0.31, E{sub c} - 0.27, and E{sub c} - 0.16 eV. Diffusion in a chlorine-containing atmosphere introduces only a single level E4, but its concentration is 2.5 times lower, compared with diffusion in air. The values of the ionization energy of the Q3 level, measured under equilibrium (Hall effect) and nonequilibrium (capacitance spectroscopy) conditions, almost coincide. The deepest level E1 with an energy of E{sub c} - 0.54 eV, formed upon diffusion in both media, has no effect on the resistivity in the n base of the structures.

  4. Cooperation of Doxycycline with Phytochemicals and Micronutrients Against Active and Persistent Forms of Borrelia sp

    PubMed Central

    Goc, Anna; Niedzwiecki, Alexandra; Rath, Matthias

    2016-01-01

    Phytochemicals and micronutrients represent a growing theme in antimicrobial defense; however, little is known about their anti-borreliae effects of reciprocal cooperation with antibiotics. A better understanding of this aspect could advance our knowledge and help improve the efficacy of current approaches towards Borrelia sp. In this study, phytochemicals and micronutrients such as baicalein, luteolin, 10-HAD, iodine, rosmarinic acid, and monolaurin, as well as, vitamins D3 and C were tested in a combinations with doxycycline for their in vitro effectiveness against vegetative (spirochetes) and latent (rounded bodies, biofilm) forms of Borrelia burgdorferi and Borrelia garinii. Anti-borreliae effects were evaluated according to checkerboard assays and supported by statistical analysis. The results showed that combination of doxycycline with flavones such as baicalein and luteolin exhibited additive effects against all morphological forms of studied Borrelia sp. Doxycycline combined with iodine demonstrated additive effects against spirochetes and biofilm, whereas with fatty acids such as monolaurin and 10-HAD it produced FICIs of indifference. Additive anti-spirochetal effects were also observed when doxycycline was used with rosmarinic acid and both vitamins D3 and C. Antagonism was not observed in any of the cases. This data revealed the intrinsic anti-borreliae activity of doxycycline with tested phytochemicals and micronutrients indicating that their addition may enhance efficacy of this antibiotic in combating Borrelia sp. Especially the addition of flavones balcalein and luteolin to a doxycycline regimen could be explored further in defining more effective treatments against these bacteria. PMID:27570483

  5. Cooperation of Doxycycline with Phytochemicals and Micronutrients Against Active and Persistent Forms of Borrelia sp.

    PubMed

    Goc, Anna; Niedzwiecki, Alexandra; Rath, Matthias

    2016-01-01

    Phytochemicals and micronutrients represent a growing theme in antimicrobial defense; however, little is known about their anti-borreliae effects of reciprocal cooperation with antibiotics. A better understanding of this aspect could advance our knowledge and help improve the efficacy of current approaches towards Borrelia sp. In this study, phytochemicals and micronutrients such as baicalein, luteolin, 10-HAD, iodine, rosmarinic acid, and monolaurin, as well as, vitamins D3 and C were tested in a combinations with doxycycline for their in vitro effectiveness against vegetative (spirochetes) and latent (rounded bodies, biofilm) forms of Borrelia burgdorferi and Borrelia garinii. Anti-borreliae effects were evaluated according to checkerboard assays and supported by statistical analysis. The results showed that combination of doxycycline with flavones such as baicalein and luteolin exhibited additive effects against all morphological forms of studied Borrelia sp. Doxycycline combined with iodine demonstrated additive effects against spirochetes and biofilm, whereas with fatty acids such as monolaurin and 10-HAD it produced FICIs of indifference. Additive anti-spirochetal effects were also observed when doxycycline was used with rosmarinic acid and both vitamins D3 and C. Antagonism was not observed in any of the cases. This data revealed the intrinsic anti-borreliae activity of doxycycline with tested phytochemicals and micronutrients indicating that their addition may enhance efficacy of this antibiotic in combating Borrelia sp. Especially the addition of flavones balcalein and luteolin to a doxycycline regimen could be explored further in defining more effective treatments against these bacteria. PMID:27570483

  6. Premature lethality, hyperactivity, and aberrant phosphorylation in transgenic mice expressing a constitutively active form of Fyn

    PubMed Central

    Xia, Di; Götz, Jürgen

    2014-01-01

    The kinase Fyn, the microtubule-associated protein tau and the peptide amyloid-β (Aβ) constitute a toxic triad in Alzheimer's disease (AD). Tau's subcellular localization is mainly regulated by phosphorylation whereas Fyn's localization is dictated by palmitoylation targeting it to the plasma membrane in a reversible manner. We have previously shown that tau is required for Fyn to be targeted to the dendritic spine. We had also shown that a truncated form of tau (Δtau) that accumulates in the cell soma is capable of trapping Fyn and preventing it from entering the spine. Here we determined that palmitoylation is required for Fyn's membrane and spine localization. We further evaluated the functional consequences of neuronal over-expression of the constitutively active Y531F mutant form of Fyn (FynCA) in transgenic mice. We found that the FynCA transgenic mice displayed a reduced weight, a massively reduced lifespan and a high level of hyperactivity. The lifespan of the FynCA mice was only slightly extended by crossing them with Δtau transgenic mice, possibly reflecting differences in expression patterns of the transgenes and high levels of transgenic FynCA compared to endogenous Fyn. Analysis of synaptosomes revealed that FynCA accumulated at high levels in the spine, resulting in increased levels of the NMDA receptor subunit NR2b phosphorylated at residue Y1472. Tau was strongly phosphorylated at the AT8 epitope S202/T205 as shown by Western blot and immunohistochemistry indicating that an increased tyrosine kinase activity of Fyn has down-stream consequences for serine/threonine-directed phosphorylation. PMID:24860422

  7. Activation of human natural killer cells by the soluble form of cellular prion protein

    SciTech Connect

    Seong, Yeon-Jae; Sung, Pil Soo; Jang, Young-Soon; Choi, Young Joon; Park, Bum-Chan; Park, Su-Hyung; Park, Young Woo; Shin, Eui-Cheol

    2015-08-21

    Cellular prion protein (PrP{sup C}) is widely expressed in various cell types, including cells of the immune system. However, the specific roles of PrP{sup C} in the immune system have not been clearly elucidated. In the present study, we investigated the effects of a soluble form of recombinant PrP{sup C} protein on human natural killer (NK) cells. Recombinant soluble PrP{sup C} protein was generated by fusion of human PrP{sup C} with the Fc portion of human IgG{sub 1} (PrP{sup C}-Fc). PrP{sup C}-Fc binds to the surface of human NK cells, particularly to CD56{sup dim} NK cells. PrP{sup C}-Fc induced the production of cytokines and chemokines and the degranulation of granzyme B from NK cells. In addition, PrP{sup C}-Fc facilitated the IL-15-induced proliferation of NK cells. PrP{sup C}-Fc induced phosphorylation of ERK-1/2 and JNK in NK cells, and inhibitors of the ERK or the JNK pathways abrogated PrP{sup C}-Fc-induced cytokine production in NK cells. In conclusion, the soluble form of recombinant PrP{sup C}-Fc protein activates human NK cells via the ERK and JNK signaling pathways. - Highlights: • Recombinant soluble PrP{sup C} (PrP{sup C}-Fc) was generated by fusion of human PrP{sup C} with IgG1 Fc portion. • PrP{sup C}-Fc protein induces the production of cytokines and degranulation from human NK cells. • PrP{sup C}-Fc protein enhances the IL-15-induced proliferation of human NK cells. • PrP{sup C}-Fc protein activates human NK cells via the ERK and JNK signaling pathways.

  8. CTEPP DATA COLLECTION FORM 09 (PERIODS 1-4 AND FOOD, FRUIT & VEG): CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data collection form is divided into two parts: Child Activity Diary and Food Survey. The Child Activity Diary collects information on the child's activities at home over the 48-hr monitoring period. The diary is divided into four time periods over the 48-hr monitoring inter...

  9. CTEPP DATA COLLECTION FORM 08 (PERIODS 1-5 AND FOOD, FRUIT & VEG): CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data collection form is divided into two parts: Child Activity Diary and Food Survey. The Child Activity Diary collects information on the child's activities at home over the 48-hr monitoring period. The diary is divided into five time periods over the 48-hr monitoring inter...

  10. Mid- to far-infrared properties of star-forming galaxies and active galactic nuclei

    NASA Astrophysics Data System (ADS)

    Magdis, G. E.; Rigopoulou, D.; Helou, G.; Farrah, D.; Hurley, P.; Alonso-Herrero, A.; Bock, J.; Burgarella, D.; Chapman, S.; Charmandaris, V.; Cooray, A.; Dai, Y. Sophia; Dale, D.; Elbaz, D.; Feltre, A.; Hatziminaoglou, E.; Huang, J.-S.; Morrison, G.; Oliver, S.; Page, M.; Scott, D.; Shi, Y.

    2013-10-01

    We study the mid- to far-IR properties of a 24 μm-selected flux-limited sample (S24> 5 mJy) of 154 intermediate redshift (⟨ z ⟩ ~ 0.15), infrared luminous galaxies, drawn from the 5 Milli-Jansky Unbiased Spitzer Extragalactic Survey. By combining existing mid-IR spectroscopy and new Herschel SPIRE submm photometry from the Herschel Multi-tiered Extragalactic Survey, we derived robust total infrared luminosity (LIR) and dust mass (Mdust) estimates and infered the relative contribution of the AGN to the infrared energy budget of the sources. We found that the total (8-1000 μm) infrared emission of galaxies with weak 6.2 μm PAH emission (EW6.2 ≤ 0.2 μm) is dominated by AGN activity, while for galaxies with EW6.2> 0.2 μm more than 50% of the LIR arises from star formation. We also found that for galaxies detected in the 250-500 μm Herschel bands an AGN has a statistically insignificant effect on the temperature of the cold dust and the far-IR colours of the host galaxy, which are primarily shaped by star formation activity. For star-forming galaxies we reveal an anti-correlation between the LIR-to-rest-frame 8 μm luminosity ratio, IR8 ≡ LIR/L8 and the strength of PAH features. We found that this anti-correlation is primarily driven by variations in the PAHsemission, and not by variations in the 5-15 μm mid-IR continuum emission. Using the [Ne iii]/[Ne ii] line ratio as a tracer of the hardness of the radiation field, we confirm that galaxies with harder radiation fields tend to exhibit weaker PAH features, and found that they have higher IR8 values and higher dust-mass-weighted luminosities (LIR/Mdust), the latter being a proxy for the dust temperature (Td). We argue that these trends originate either from variations in the environment of the star-forming regions or are caused by variations in the age of the starburst. Finally, we provide scaling relations that will allow estimating LIR, based on single-band observations with the mid-infrared instrument

  11. Characterization of a soluble, catalytically active form of Escherichia coli leader peptidase: requirement of detergent or phospholipid for optimal activity.

    PubMed

    Tschantz, W R; Paetzel, M; Cao, G; Suciu, D; Inouye, M; Dalbey, R E

    1995-03-28

    Leader peptidase is a novel serine protease in Escherichia coli, which functions to cleave leader sequences from exported proteins. Its catalytic domain extends into the periplasmic space and is anchored to the membrane by two transmembrane segments located at the N-terminal end of the protein. At present, there is no information on the structure of the catalytic domain. Here, we report on the properties of a soluble form of leader peptidase (delta 2-75), and we compare its properties to those of the wild-type enzyme. We find that the truncated leader peptidase has a kcat of 3.0 S-1 and a Km of 32 microM with a pro-OmpA nuclease A substrate. In contrast to the wild-type enzyme (pI of 6.8), delta 2-75 is water-soluble and has an acidic isoelectric point of 5.6. We also show with delta 2-75 that the replacement of serine 90 and lysine 145 with alanine residues results in a 500-fold reduction in activity, providing further evidence that leader peptidase employs a catalytic serine/lysine dyad. Finally, we find that the catalysis of delta 2-75 is accelerated by the presence of the detergent Triton X-100, regardless if the substrate is pro-OmpA nuclease A or a peptide substrate. Triton X-100 is required for optimal activity of delta 2-75 at a level far below the critical micelle concentration. Moreover, we find that E. coli phospholipids stimulate the activity of delta 2-75, suggesting that phospholipids may play an important physiological role in the catalytic mechanism of leader peptidase. PMID:7696258

  12. Optogenetic activation of presynaptic inputs in lateral amygdala forms associative fear memory

    PubMed Central

    Kwon, Jeong-Tae; Nakajima, Ryuichi; Kim, Hyung-Su; Jeong, Yire; Augustine, George J.

    2014-01-01

    In Pavlovian fear conditioning, the lateral amygdala (LA) has been highlighted as a key brain site for association between sensory cues and aversive stimuli. However, learning-related changes are also found in upstream sensory regions such as thalamus and cortex. To isolate the essential neural circuit components for fear memory association, we tested whether direct activation of presynaptic sensory inputs in LA, without the participation of upstream activity, is sufficient to form fear memory in mice. Photostimulation of axonal projections from the two main auditory brain regions, the medial geniculate nucleus of the thalamus and the secondary auditory cortex, was paired with aversive footshock. Twenty-four hours later the same photostimulation induced robust conditioned freezing and this fear memory formation was disrupted when glutamatergic synaptic transmission was locally blocked in the LA. Therefore, our results prove for the first time that synapses between sensory input areas and the LA, previously implicated as a crucial brain site for fear memory formation, actually are sufficient to serve as a conditioned stimulus. Our results strongly support the idea that the LA may be sufficient to encode and store associations between neutral cue and aversive stimuli during natural fear conditioning as a critical part of a broad fear memory engram. PMID:25322798

  13. T-lymphocyte activation and the cellular form of the prion protein.

    PubMed Central

    Mabbott, N A; Brown, K L; Manson, J; Bruce, M E

    1997-01-01

    The transmissible spongiform encephalopathies are neurodegenerative disorders which include Creutzfeldt-Jakob disease in humans, and scrapie and bovine spongiform encephalopathy in animals. A major component of the infectious agent responsible for these diseases is considered to be a post-translationally modified form of a host-encoded glycoprotein PrPc, termed PrPSc. While PrPc is abundantly expressed in tissues of the central nervous system (CNS), little is known about its normal function. The expression of PrPc is not restricted to the CNS, as this protein can also be detected in the lymphoid tissues of mice and sheep. In this report we demonstrate that resting murine splenic lymphocytes express PrPc protein on their cell membranes. Furthermore, expression of PrPc was significantly enhanced following in vitro stimulation with the non-specific T-cell mitogen concanavalin A (Con A). Genetically engineered mice with an inactive PrPc gene (PrP-/- mice), were utilized to investigate the involvement of PrPc in lymphocyte activation. Experiments revealed that the Con A-induced proliferation of lymphocytes from PrP-/- mice was significantly reduced to approximately 50-80% that of wild-type (PrP+/+) mice 48 hr post-stimulation. These findings demonstrate an important role for PrPc in extra-neuronal tissues and suggest that PrPc is a lymphocyte surface molecule that participates in T-cell activation. PMID:9415021

  14. WIDESPREAD AND HIDDEN ACTIVE GALACTIC NUCLEI IN STAR-FORMING GALAXIES AT REDSHIFT >0.3

    SciTech Connect

    Juneau, Stephanie; Bournaud, Frederic; Daddi, Emanuele; Elbaz, David; Alexander, David M.; Mullaney, James R.; Magnelli, Benjamin; Hwang, Ho Seong; Willner, S. P.; Coil, Alison L.; Rosario, David J.; Trump, Jonathan R.; Faber, S. M.; Kocevski, Dale D.; Cooper, Michael C.; Frayer, David T.; and others

    2013-02-20

    We characterize the incidence of active galactic nuclei (AGNs) in 0.3 < z < 1 star-forming galaxies by applying multi-wavelength AGN diagnostics (X-ray, optical, mid-infrared, radio) to a sample of galaxies selected at 70 {mu}m from the Far-Infrared Deep Extragalactic Legacy survey (FIDEL). Given the depth of FIDEL, we detect 'normal' galaxies on the specific star formation rate (sSFR) sequence as well as starbursting systems with elevated sSFR. We find an overall high occurrence of AGN of 37% {+-} 3%, more than twice as high as in previous studies of galaxies with comparable infrared luminosities and redshifts but in good agreement with the AGN fraction of nearby (0.05 < z < 0.1) galaxies of similar infrared luminosities. The more complete census of AGNs comes from using the recently developed Mass-Excitation (MEx) diagnostic diagram. This optical diagnostic is also sensitive to X-ray weak AGNs and X-ray absorbed AGNs, and reveals that absorbed active nuclei reside almost exclusively in infrared-luminous hosts. The fraction of galaxies hosting an AGN appears to be independent of sSFR and remains elevated both on the sSFR sequence and above. In contrast, the fraction of AGNs that are X-ray absorbed increases substantially with increasing sSFR, possibly due to an increased gas fraction and/or gas density in the host galaxies.

  15. Alarin but not its alternative-splicing form, GALP (Galanin-like peptide) has antimicrobial activity

    SciTech Connect

    Wada, Akihiro; Wong, Pooi-Fong; Hojo, Hironobu; Hasegawa, Makoto; Ichinose, Akitoyo; Llanes, Rafael; Kubo, Yoshinao; Senba, Masachika; Ichinose, Yoshio

    2013-05-03

    Highlights: • Alarin inhibits the growth of E. coli but not S. aureus. • Alarin’s potency is comparable to LL-37 in inhibiting the growth of E. coli. • Alarin can cause bacterial membrane blebbing. • Alalin does not induce hemolysis on erythrocytes. -- Abstract: Alarin is an alternative-splicing form of GALP (galanin-like peptide). It shares only 5 conserved amino acids at the N-terminal region with GALP which is involved in a diverse range of normal brain functions. This study seeks to investigate whether alarin has additional functions due to its differences from GALP. Here, we have shown using a radial diffusion assay that alarin but not GALP inhibited the growth of Escherichia coli (strain ML-35). The conserved N-terminal region, however, remained essential for the antimicrobial activity of alarin as truncated peptides showed reduced killing effect. Moreover, alarin inhibited the growth of E. coli in a similar potency as human cathelicidin LL-37, a well-studied antimicrobial peptide. Electron microscopy further showed that alarin induced bacterial membrane blebbing but unlike LL-37, it did not cause hemolysis of erythrocytes. In addition, alarin is only active against the gram-negative bacteria, E. coli but not the gram-positive bacteria, Staphylococcus aureus. Thus, these data suggest that alarin has potentials as an antimicrobial and should be considered for the development in human therapeutics.

  16. Photoluminescence Mechanism and Photocatalytic Activity of Organic-Inorganic Hybrid Materials Formed by Sequential Vapor Infiltration.

    PubMed

    Akyildiz, Halil I; Stano, Kelly L; Roberts, Adam T; Everitt, Henry O; Jur, Jesse S

    2016-05-01

    Organic-inorganic hybrid materials formed by sequential vapor infiltration (SVI) of trimethylaluminum into polyester fibers are demonstrated, and the photoluminescence of the fibers is evaluated using a combined UV-vis and photoluminescence excitation (PLE) spectroscopy approach. The optical activity of the modified fibers depends on infiltration thermal processing conditions and is attributed to the reaction mechanisms taking place at different temperatures. At low temperatures a single excitation band and dual emission bands are observed, while, at high temperatures, two distinct absorption bands and one emission band are observed, suggesting that the physical and chemical structure of the resulting hybrid material depends on the SVI temperature. Along with enhancing the photoluminescence intensity of the PET fibers, the internal quantum efficiency also increased to 5-fold from ∼4-5% to ∼24%. SVI processing also improved the photocatalytic activity of the fibers, as demonstrated by photodeposition of Ag and Au metal particles out of an aqueous metal salt solution onto fiber surfaces via UVA light exposure. Toward applications in flexible electronics, well-defined patterning of the metallic materials is achieved by using light masking and focused laser rastering approaches. PMID:27063955

  17. Does the proposed DSE motif form the active center in the Hermes transposase?

    PubMed

    Michel, K; O'Brochta, D A; Atkinson, P W

    2002-10-01

    Donor cleavage and strand transfer are two functions performed by transposases during transposition of class II transposable elements. Within transposable elements, the only active center described, to date, facilitating both functions, is the so-called DDE motif. A second motif, R-K-H/K-R-H/W-Y, is found in the site-specific recombinases of the tyrosine recombinase family. While present in many bacterial insertion sequences as well as in the eukaryotic family of mariner/Tc1 elements, the DDE motif was considered absent in other classes of eukaryotic class II elements such as P, and hAT and piggyBac. Based on sequence alignments of a hobo-like element from the nematode Caenorhabditis elegans, to a variety of other hAT transposases and several members of the mariner/Tc1 group, Bigot et al. [Gene 174 (1996) 265] proposed the presence of a DSE motif in hAT transposases. In the present study we tested if each of these three residues is required for transposition of the Hermes element, a member of the hAT family commonly used for insect transformation. While D402N and E572Q mutations lead to knock-out of Hermes function, mutations S535A and S535D did not affect transposition frequency or the choice of integration sites. These data give the first experimental support that D402 and E572 are indeed required for transposition of Hermes. Furthermore, this study indicates that the active center of the Hermes transposase differs from the proposed DSE motif. It remains to be shown if other residues also form the active site of this transposase. PMID:12426102

  18. Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes.

    PubMed

    Crofts, Naoko; Abe, Natsuko; Oitome, Naoko F; Matsushima, Ryo; Hayashi, Mari; Tetlow, Ian J; Emes, Michael J; Nakamura, Yasunori; Fujita, Naoko

    2015-08-01

    Amylopectin is a highly branched, organized cluster of glucose polymers, and the major component of rice starch. Synthesis of amylopectin requires fine co-ordination between elongation of glucose polymers by soluble starch synthases (SSs), generation of branches by branching enzymes (BEs), and removal of misplaced branches by debranching enzymes (DBEs). Among the various isozymes having a role in amylopectin biosynthesis, limited numbers of SS and BE isozymes have been demonstrated to interact via protein-protein interactions in maize and wheat amyloplasts. This study investigated whether protein-protein interactions are also found in rice endosperm, as well as exploring differences between species. Gel permeation chromatography of developing rice endosperm extracts revealed that all 10 starch biosynthetic enzymes analysed were present at larger molecular weights than their respective monomeric sizes. SSIIa, SSIIIa, SSIVb, BEI, BEIIb, and PUL co-eluted at mass sizes >700kDa, and SSI, SSIIa, BEIIb, ISA1, PUL, and Pho1 co-eluted at 200-400kDa. Zymogram analyses showed that SSI, SSIIIa, BEI, BEIIa, BEIIb, ISA1, PUL, and Pho1 eluted in high molecular weight fractions were active. Comprehensive co-immunoprecipitation analyses revealed associations of SSs-BEs, and, among BE isozymes, BEIIa-Pho1, and pullulanase-type DBE-BEI interactions. Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes. These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes. Detailed analyses of these complexes will shed light on the mechanisms controlling the unique branch and cluster structure of amylopectin, and the physicochemical properties of starch. PMID:25979995

  19. Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes

    PubMed Central

    Crofts, Naoko; Abe, Natsuko; Oitome, Naoko F.; Matsushima, Ryo; Hayashi, Mari; Tetlow, Ian J.; Emes, Michael J.; Nakamura, Yasunori; Fujita, Naoko

    2015-01-01

    Amylopectin is a highly branched, organized cluster of glucose polymers, and the major component of rice starch. Synthesis of amylopectin requires fine co-ordination between elongation of glucose polymers by soluble starch synthases (SSs), generation of branches by branching enzymes (BEs), and removal of misplaced branches by debranching enzymes (DBEs). Among the various isozymes having a role in amylopectin biosynthesis, limited numbers of SS and BE isozymes have been demonstrated to interact via protein–protein interactions in maize and wheat amyloplasts. This study investigated whether protein–protein interactions are also found in rice endosperm, as well as exploring differences between species. Gel permeation chromatography of developing rice endosperm extracts revealed that all 10 starch biosynthetic enzymes analysed were present at larger molecular weights than their respective monomeric sizes. SSIIa, SSIIIa, SSIVb, BEI, BEIIb, and PUL co-eluted at mass sizes >700kDa, and SSI, SSIIa, BEIIb, ISA1, PUL, and Pho1 co-eluted at 200–400kDa. Zymogram analyses showed that SSI, SSIIIa, BEI, BEIIa, BEIIb, ISA1, PUL, and Pho1 eluted in high molecular weight fractions were active. Comprehensive co-immunoprecipitation analyses revealed associations of SSs–BEs, and, among BE isozymes, BEIIa–Pho1, and pullulanase-type DBE–BEI interactions. Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes. These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes. Detailed analyses of these complexes will shed light on the mechanisms controlling the unique branch and cluster structure of amylopectin, and the physicochemical properties of starch. PMID:25979995

  20. 77 FR 65706 - Agency Information Collection Activities: Immigrant Petition for Alien Worker, Form I-140...

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    2012-10-30

    ... Petition for Alien Worker, Form I-140; Revision of a Currently Approved Collection ACTION: 60-Day Notice... Form/Collection: Immigrant Petition for Alien Worker. (3) Agency form number, if any, and the... information furnished on Form I-140 will be used by USCIS to classify aliens under sections 203(b)(1),...

  1. An oligomeric form of E. coli UvrD is required for optimal helicase activity.

    PubMed

    Ali, J A; Maluf, N K; Lohman, T M

    1999-11-01

    Pre-steady-state chemical quenched-flow techniques were used to study DNA unwinding catalyzed by Escherichia coli UvrD helicase (helicase II), a member of the SF1 helicase superfamily. Single turnover experiments, with respect to unwinding of a DNA oligonucleotide, were used to examine the DNA substrate and UvrD concentration requirements for rapid DNA unwinding by pre-bound UvrD helicase. In excess UvrD at low DNA concentrations (1 nM), the bulk of the DNA is unwound rapidly by pre-bound UvrD complexes upon addition of ATP, but with time-courses that display a distinct lag phase for formation of fully unwound DNA, indicating that unwinding occurs in discrete steps, with a "step size" of four to five base-pairs as previously reported. Optimum unwinding by pre-bound UvrD-DNA complexes requires a 3' single-stranded (ss) DNA tail of 36-40 nt, whereas productive complexes do not form readily on DNA with 3'-tail lengths form of the UvrD helicase with optimal helicase activity is oligomeric with at least two sites for binding the DNA substrate, where one site contacts regions of the 3'-ssDNA tail that are distal from the single-stranded/double-stranded DNA junction. PMID:10543970

  2. Light-activated, in situ forming gel for sustained suprachoroidal delivery of bevacizumab.

    PubMed

    Tyagi, Puneet; Barros, Matthew; Stansbury, Jeffrey W; Kompella, Uday B

    2013-08-01

    A light-activated polycaprolactone dimethacrylate (PCM) and hydroxyethyl methacrylate (HEMA) based gel network was developed to sustain the release of stable, active bevacizumab (an anti-VEGF antibody used to treat choroidal neovascularization) and used to assess sustained ex vivo delivery in rabbit eyes and in vivo delivery in rat eyes following in situ gel formation in the suprachoroidal space. PCM was synthesized from polycaprolactone diol (PCD) and evaluated using NMR spectroscopy. PCM was used to cross-link HEMA in the presence of 365 nm UV light and 2,2-dimethoxy-2-phenylacetophenone (DMPA) as a photoinitiator. Bevacizumab was entrapped in the gel using three different cross-linking durations of 3, 7, and 10 min. In vitro release of bevacizumab in PBS pH 7.4 at 37 °C during a 4 month study was quantified using a VEGF-binding based ELISA. The stability of released bevacizumab was monitored by size exclusion chromatography (SEC) and circular dichroism. Alexa Fluor 488 dye conjugated bevacizumab mixed with polymers was injected suprachoroidally in rabbit eyes to study the effect of different cross-linking durations on the spread of the dye conjugated bevacizumab. In vivo delivery was assessed in Sprague-Dawley (SD) rats by injecting Alexa Fluor 488 dye conjugated bevacizumab mixed with polymers followed by cross-linking for 10 min. Spread in the rabbit eyes and in vivo delivery in rat eyes was monitored noninvasively using a fundus camera and Fluorotron Master. The formation of PCM was confirmed by the disappearance of hydroxyl peak in NMR spectra. A cross-linking duration of 10 min resulted in a burst release of 21% of bevacizumab. Other cross-linking durations had ≥62% burst release. Bevacizumab release from 10 min cross-linked gel was sustained for ∼4 months. Release samples contained ≥96.1% of bevacizumab in the monomeric form as observed in SEC chromatograms. Circular dichroism confirmed that secondary β-sheet structure of bevacizumab was maintained

  3. 77 FR 23735 - Agency Information Collection Activities: Forms G-1041 and G-1041A, Extension of a Currently...

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    2012-04-20

    ..., 2012, at 77 FR 9259, allowing for a 60-day public comment period. USCIS did not receive any comments... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Forms G-1041... Notice of Information Collection Under Review: Forms G- 1041 and G-1041A, Genealogy Index Search...

  4. 77 FR 64388 - Agency Information Collection (Former POW Medical History), VA Form 10-0048 Activities Under OMB...

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    2012-10-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF VETERANS AFFAIRS Agency Information Collection (Former POW Medical History), VA Form 10-0048 Activities Under OMB....'' SUPPLEMENTARY INFORMATION: Title: Former POW Medical History, VA Form 10-0048. OMB Control Number:...

  5. 77 FR 27241 - Agency Information Collection Activities: Form I-918, and Supplement A and B, Extension of a...

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    2012-05-09

    ... SECURITY U.S. Citizenship and Immigration Services Agency Information Collection Activities: Form I-918... Status; and Supplement A and B. The Department of Homeland Security, U.S. Citizenship and Immigration... sponsoring the collection: Form I-918 and Supplements A and B; U.S. Citizenship and Immigration Services....

  6. A Review of Recent RTO Benefit-Cost Studies: Toward MoreComprehensive Assessments of FERC Electricity RestructuringPolicies

    SciTech Connect

    Eto, Joseph H.; Lesieutre, Bernard C.

    2005-12-01

    During the past three years, government and private organizations have issued more than a dozen studies of the benefits and costs of Regional Transmission Organizations (RTOs). Most of these studies have focused on benefits that can be readily estimated using traditional production-cost simulation techniques, which compare the cost of centralized dispatch under an RTO to dispatch in the absence of an RTO, and on costs associated with RTO start-up and operation. Taken as a whole, it is difficult to draw definitive conclusions from these studies because they have not examined potentially much larger benefits (and costs) resulting from the impacts of RTOs on reliability management, generation and transmission investment and operation, and wholesale electricity market operation. This report: (1) Describes the history of benefit-cost analysis of FERC electricity restructuring policies; (2)Reviews current practice by analyzing 11 RTO benefit-cost studies that were published between 2002 and 2004 and makes recommendations to improve the documentation of data and methods and the presentation of findings in future studies that focus primarily on estimating short-run economic impacts; and (3) Reviews important impacts of FERC policies that have been overlooked or incompletely treated by recent RTO benefit-cost studies and the challenges to crafting more comprehensive assessments of these impacts based on actual performance, including impacts on reliability management, generation and transmission investment and operation, and wholesale electricity market operation.

  7. Dissociation of membrane binding and lytic activities of the lymphocyte pore-forming protein (perforin).

    PubMed

    Young, J D; Damiano, A; DiNome, M A; Leong, L G; Cohn, Z A

    1987-05-01

    Granules isolated from CTL and NK cells contain a cytolytic pore-forming protein (PFP/perforin). At low temperatures (on ice), PFP binds to erythrocyte membranes without producing hemolysis. Hemolysis occurs when the PFP-bound erythrocytes are warmed up to 37 degrees C, which defines a temperature-dependent, lytic (pore-formation) step distinct from the membrane-binding event. Ca2+ and neutral pH are required for both membrane binding and pore formation by PFP. Serum, LDL, HDL, and heparin inhibit the hemolytic activity of PFP by blocking its binding to lipid membranes. Lysis by PFP that has bound to erythrocyte membranes is no longer susceptible to the effect of these inhibitors. The hemolytic activities associated with intact granules and solubilized PFP show different requirements for Ca2+ and pH, indicating that cytolysis produced by isolated granules may involve an additional step, possibly fusion of granules with membranes. It is suggested that three distinct Ca2+- and pH-dependent events may be involved during cell killing by CTL and NK cells: fusion of cytoplasmic granules of effector cells with their plasma membrane, releasing PFP from cells; binding of the released PFP to target membranes; and insertion of monomers and the subsequent formation of lytic pores in the target membrane. The serum-mediated inhibition of membrane binding by PFP could prevent the accidental injury of bystander cells by cell-released PFP, but would allow cytolysis to proceed to completion once PFP has bound to the target membrane. PMID:3494808

  8. 77 FR 71432 - Agency Information Collection Activities: Immigrant Petition by Alien Entrepreneur, Form I-526...

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  9. 77 FR 36285 - Agency Information Collection Activities: Form I-693, Revision of a Currently Approved...

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    ... Information Collection Under Review: Form I- 693, Report of Medical Examination and Vaccination Record, OMB... Medical Examination and Vaccination Record. (3) Agency form number, if any, and the applicable...

  10. 76 FR 24908 - Agency Information Collection Activities: Form I-693, Revision of a Currently Approved...

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    2011-05-03

    ... Information Collection Under Review: Form I- 693, Report of Medical Examination and Vaccination Record, OMB... Vaccination Record. (3) Agency form number, if any, and the applicable component of the Department of...

  11. 76 FR 51997 - Agency Information Collection Activities: Form I-602; Extension of an Existing Information...

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    2011-08-19

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  12. Scube/You activity mediates release of dually lipid-modified Hedgehog signal in soluble form

    PubMed Central

    Creanga, Adrian; Glenn, Thomas D.; Mann, Randall K.; Saunders, Adam M.; Talbot, William S.; Beachy, Philip A.

    2012-01-01

    Owing to their covalent modification by cholesterol and palmitate, Hedgehog (Hh) signaling proteins are localized predominantly to the plasma membrane of expressing cells. Yet Hh proteins are also capable of mobilizing to and eliciting direct responses from distant cells. The zebrafish you gene, identified genetically >15 years ago, was more recently shown to encode a secreted glycoprotein that acts cell-nonautonomously in the Hh signaling pathway by an unknown mechanism. We investigated the function of the protein encoded by murine Scube2, an ortholog of you, and found that it mediates release in soluble form of the mature, cholesterol- and palmitate-modified Sonic hedgehog protein signal (ShhNp) when added to cultured cells or purified detergent-resistant membrane microdomains containing ShhNp. The efficiency of Scube2-mediated release of ShhNp is enhanced by the palmitate adduct of ShhNp and by coexpression in ShhNp-producing cells of mDispatchedA (mDispA), a transporter-like protein with a previously defined role in the release of lipid-modified Hh signals. The structural determinants of Scube2 required for its activity in cultured cell assays match those required for rescue of you mutant zebrafish embryos, and we thus conclude that the role of Scube/You proteins in Hh signaling in vivo is to facilitate the release and mobilization of Hh proteins for distant action. PMID:22677548

  13. [Anti-adenovirus activity of a substance and medical form of ribamydil in cell culture].

    PubMed

    Nosach, L N; Diachenko, N S; Zhovnovataia, V L

    2009-01-01

    The inhibiting effect of ribamydil on adenovirus reproduction was studied under the determination of the number of cells with virus- induced DNA-containing intranucleus inclusion bodies and hexone antigen, the synthesis of adenovirus proteins and the infection virus by t he investigation. EC50 of ribamydil substance is 4-8 microg/ml, but complete suppression of adenovirus genome expression was found when adding ribamydil after the virus adsorption, in concentrations of 125-500 microg/ml. The original effect of ribamydil on the expression of adenovirus genome was found under its effect in concentration of 31 microg/ml. Intranucleus virus-induced inclusion bodies of the early type only were found under these conditions. Synthesis of the structural virus polypeptides, including hexone polypeptide (II) and non-structural polypeptide 100K, taking part in hexone trimerization, proceed intensively but without formation of immunologically active hexone. The inhibiting effect of officinal form of ribamydil was less expressed as compared with the substance (EC50: 62 microg/ml). The work results prove that the therapeutic effect of ribamydil (ribavirin) under treatment of adenovirus infections may be achieved in case when it is used in a dose excluding the expression of the adenovirus genome. PMID:20458939

  14. 77 FR 33758 - Agency Information Collection Activities: Form I-829, Extension of a Currently Approved...

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  16. 75 FR 71451 - Agency Information Collection Activities: Form I-130, Extension of a Currently Approved...

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    2010-11-23

    ... published in the Federal Register on August 26, 2010, at 75 FR 52540, allowing for a 60-day public comment... Information Collection Under Review: Form I- 130, Petition for Alien Relative; OMB Control No. 1615-0012. The...) Title of the Form/Collection: Petition for Alien Relative. (3) Agency form number, if any, and...

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    2011-03-28

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  18. 75 FR 11192 - Agency Information Collection Activities: Form I-730, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-10

    ... Information Collection under Review: Form I- 730, Refugee/Asylee Relative Petition. The Department of Homeland... collection. (2) Title of the Form/Collection: Refugee/Asylee Relative Petition. (3) Agency form number, if... used by an asylee or refugee to file on behalf of his or her spouse and/or children provided that...

  19. 75 FR 5101 - Agency Information Collection Activities: Form I-590, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... was previously published in the Federal Register onOctober 16, 2009, at 74 FR 53285, allowing for a 60... information collection under review: Form I- 590, Registratiod for Classification as Refugee; OMB Control No...) Title of the Form/Collection: Registration for Classification as Refugee. (3) Agency form number, if...

  20. 75 FR 31460 - Agency Information Collection Activities: Form I-730, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... previously published in the Federal Register on March 10, 2010 at 75 FR 11192, allowing for a 60- day public... Information Collection Under Review: Form I- 730, Refugee/Asylee Relative Petition. OMB Control No. 1615-0037... the Form/Collection: Refugee/Asylee Relative Petition. (3) Agency form number, if any, and...