Sample records for activities ferc form

  1. 78 FR 26766 - Commission Information Collection Activities (FERC Form 580); Comment Request; Revision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-08

    ... Information Collection Activities (FERC Form 580); Comment Request; Revision AGENCY: Federal Energy Regulatory... collection, FERC Form No. 580 (Interrogatory on Fuel and Energy Purchase Practices). DATES: Comments on the... (FERC Form No. 580). OMB Control No.: 1902-0131. Type of Request: Three-year approval of the FERC Form...

  2. 75 FR 21623 - Commission Information Collection Activities (FERC Form Nos. 6 and 6-Q); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-26

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket Nos. IC10-6-001 and IC10-6Q-001] Commission Information Collection Activities (FERC Form Nos. 6 and 6-Q); Comment Request; Submitted for OMB... (75FR5061, 2/1/ 2010) requesting public comments. FERC received one comment on the FERC Form No. 6 and FERC...

  3. 75 FR 35776 - Commission Information Collection Activities (FERC Form No. 80); Comment Request; Submitted for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... Information Collection Activities (FERC Form No. 80); Comment Request; Submitted for OMB Review June 16, 2010... interested person may file comments directly with OMB and should address a copy of those comments to the.../2010) requesting public comments. FERC received no comments on the FERC Form No. 80 and has made this...

  4. 78 FR 45919 - Commission Information Collection Activities (FERC Form 80); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ... Information Collection Activities (FERC Form 80); Comment Request; Extension AGENCY: Federal Energy Regulatory... Office of Information and Regulatory Affairs: [email protected] . Attention: Federal Energy... receiving automatic notification of activity in this docket or in viewing/downloading comments and issuances...

  5. 78 FR 24402 - Commission Information Collection Activities (FERC Form 80); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-25

    ... Information Collection Activities (FERC Form 80); Comment Request; Extension AGENCY: Federal Energy Regulatory... automatic notification of activity in this docket or in viewing/downloading comments and issuances in this... jurisdiction to recognize the resources that are affected by their activities and to play a role in protecting...

  6. 78 FR 28820 - Commission Information Collection Activities (FERC Form 80); Comment Request; Revision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ... Information Collection Activities (FERC Form 80); Comment Request; Revision AGENCY: Federal Energy Regulatory... notification of activity in this docket or in viewing/downloading comments and issuances in this docket may do... the resources that are affected by their activities and to play a role in protecting such resources...

  7. 75 FR 38798 - Commission Information Collection Activities (FERC Form No. 580) Request; Submitted for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ..., Office of the Deputy Chief Information Officer, Federal Energy Regulatory Commission, 888 First Street... Information Collection Activities (FERC Form No. 580) Request; Submitted for OMB Review; Correction June 29... submission of information collection to the Office of Management and Budget for review of information...

  8. 78 FR 43871 - Commission Information Collection Activities (FERC-580); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-22

    ... submitting the information collection, FERC Form No. 580 (Interrogatory on Fuel and Energy Purchase Practices.... SUPPLEMENTARY INFORMATION: Title: Interrogatory on Fuel and Energy Purchase Practices (FERC Form No. 580), OMB...: Three-year approval of the FERC Form No. 580. Abstract: FERC Form No. 580 is collected in even numbered...

  9. 76 FR 46781 - Commission Information Collection Activities (FERC Form 1 and FERC Form 1F); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... Commission's chart of accounts and related accounting instructions permits comparability and financial... industry, would be difficult to achieve if each company maintained its own accounting records using dissimilar accounting methods and classifications to record similar transactions and events. The FERC Annual...

  10. 75 FR 5061 - Commission Information Collection Activities (FERC Form Nos. 6 and 6-Q); Comment Request; Extensions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket Nos. IC10-6-000 and IC10-6Q-000] Commission Information Collection Activities (FERC Form Nos. 6 and 6-Q); Comment Request; Extensions January... to Docket Nos. IC10-6-000 and IC10-6Q-000. For comments that only pertain to one of the collections...

  11. 78 FR 9679 - Commission Information Collection Activities (FERC-60, FERC-61, & FERC-555A); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-11

    ...: Three-year extension of the FERC-60, FERC-61, & FERC-555A information collection requirements with no... company's functions during the prior calendar year (FERC-61). In complying, a holding company may make a... level of transparency that: (1) Helps protect ratepayers from pass-through of improper service company...

  12. 77 FR 8245 - Commission Information Collection Activities (FERC-65, FERC-65A, and FERC-65B); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-14

    ... collections, FERC-65, FERC-65A, and FERC-65B (Notification of Holding Company Status, Exemption Notification of Holding Company Status, and Waiver Notification of Holding Company Status). DATES: Comments on the... (202) 273-0873. SUPPLEMENTARY INFORMATION: Title: Notification of Holding Company Status (FERC-65...

  13. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... FERC Form No. 715, Annual Transmission Planning and Evaluation Report. (a) Who must file. Any... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 715, Annual Transmission Planning and Evaluation Report. 141.300 Section 141.300 Conservation of Power and...

  14. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 6-Q... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the reporting...

  15. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false FERC Form No. 6-Q... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the reporting...

  16. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false FERC Form No. 6-Q... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the reporting...

  17. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false FERC Form No. 6-Q... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the reporting...

  18. 18 CFR 357.4 - FERC Form No. 6-Q, Quarterly report of oil pipeline companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 6-Q... No. 6-Q, Quarterly report of oil pipeline companies. (a) Prescription. The quarterly financial report form of oil pipeline companies, designated as FERC Form No. 6-Q, is prescribed for the reporting...

  19. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false FERC Form No. 3-Q... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q... as FERC Form No. 3-Q, is prescribed for the reporting quarter ending March 31, 2004, and each quarter...

  20. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false FERC Form No. 3-Q... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q... as FERC Form No. 3-Q, is prescribed for the reporting quarter ending March 31, 2004, and each quarter...

  1. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false FERC Form No. 3-Q... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q... as FERC Form No. 3-Q, is prescribed for the reporting quarter ending March 31, 2004, and each quarter...

  2. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 3-Q... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q... as FERC Form No. 3-Q, is prescribed for the reporting quarter ending March 31, 2004, and each quarter...

  3. 77 FR 25156 - Commission Information Collection Activities (FERC-65, FERC-65A, and FERC-65B); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-27

    ... companies. Filings may be made in hardcopy or electronically through the Commission's Web site. Type of...) is submitting the information collections Notification of Holding Company Status (FERC-65), Exemption Notification of Holding Company Status (FERC-65A), and Waiver Notification of Holding Company Status (FERC-65B...

  4. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false FERC Form No. 3-Q... AND REPORTS (SCHEDULES) § 141.400 FERC Form No. 3-Q, Quarterly financial report of electric utilities..., licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the reporting...

  5. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false FERC Form No. 3-Q... AND REPORTS (SCHEDULES) § 141.400 FERC Form No. 3-Q, Quarterly financial report of electric utilities..., licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the reporting...

  6. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false FERC Form No. 3-Q... AND REPORTS (SCHEDULES) § 141.400 FERC Form No. 3-Q, Quarterly financial report of electric utilities..., licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the reporting...

  7. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC Form No. 3-Q... AND REPORTS (SCHEDULES) § 141.400 FERC Form No. 3-Q, Quarterly financial report of electric utilities..., licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the reporting...

  8. 77 FR 73631 - Commission Information Collection Activities (FERC-500 and FERC-505); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ..., identified by the OMB Control Nos. 1902-0058 (FERC-500) and/or 1902-0114 (FERC-505), should be sent via email.../Relicense for Water Projects with 5 Megawatt or Less Capacity. OMB Control Nos.: FERC-500 (1902-0058); FERC... licenses for non-federal hydroelectric plants. 16 U.S.C. 797(e) also requires the Commission to give equal...

  9. 76 FR 35870 - Commission Information Collection Activities (FERC-523); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ..., such as administrative costs and the cost for information technology. Indirect or overhead costs are... other technological collection techniques or other forms of information technology e.g. permitting... Information Collection Activities (FERC-523); Comment Request; Extension AGENCY: Federal Energy Regulatory...

  10. 75 FR 7466 - Commission Information Collection Activities (FERC-912 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-19

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IC09-912-001] Commission Information Collection Activities (FERC-912 \\1\\), Supplemental Notice February 3, 2010. AGENCY: Federal Energy Regulatory Commission. \\1\\ FERC-912 (``Cogeneration and Small Power Production, PURPA Section 210(m...

  11. 18 CFR 131.80 - FERC Form No. 556, Certification of qualifying facility status for an existing or a proposed...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC Form No. 556... COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978 FORMS § 131.80 FERC Form No. 556, Certification of qualifying facility status for an existing...

  12. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978 FORMS § 131.31 FERC Form No. 561, Annual report of interlocking... the Federal Energy Regulatory Commission's staff for the review and oversight of interlocking...

  13. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978 FORMS § 131.31 FERC Form No. 561, Annual report of interlocking... the Federal Energy Regulatory Commission's staff for the review and oversight of interlocking...

  14. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978 FORMS § 131.31 FERC Form No. 561, Annual report of interlocking... the Federal Energy Regulatory Commission's staff for the review and oversight of interlocking...

  15. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, FEDERAL POWER ACT AND PUBLIC UTILITY REGULATORY POLICIES ACT OF 1978 FORMS § 131.31 FERC Form No. 561, Annual report of interlocking... the Federal Energy Regulatory Commission's staff for the review and oversight of interlocking...

  16. 76 FR 34216 - Commission Information Collection Activities (Ferc-521), Proposed Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-13

    ... Information Collection Activities (Ferc-521), Proposed Collection; Comment Request; Submitted for OMB Review... information collection described below. DATES: Comments in consideration of the collection of information are... comments and FERC issuances may be viewed, printed or downloaded remotely through FERC's eLibrary at http...

  17. 77 FR 58115 - Commission Information Collection Activities (FERC-546); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-19

    ... information collection FERC-546 (Certificated Filings: Gas Pipeline Rates) to the Office of Management and... fax at (202) 273-0873. SUPPLEMENTARY INFORMATION: Title: FERC-546, Certificated Filings: Gas Pipeline... gas storage, and unbundled sales activities of interstate natural gas pipelines and Hinshaw \\1...

  18. 78 FR 28825 - Commission Information Collection Activities (FERC-577); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IC13-12-000] Commission Information Collection Activities (FERC-577); Comment Request AGENCY: Federal Energy Regulatory Commission... Act of 1995, 44 USC 3507(a)(1)(D), the Federal Energy Regulatory Commission (Commission or FERC) is...

  19. 75 FR 2124 - Commission Information Collection Activities (FERC-538, FERC-539, FERC-577, FERC-606, and FERC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-14

    ... Act of 1995 (Pub. L. No. 104-13), the Federal Energy Regulatory Commission (Commission or FERC) is... the service which the applicant has requested the Commission to direct the natural gas pipeline company to render (such as a request to direct a natural gas company to extend or improve its...

  20. 76 FR 43996 - Commission Information Collection Activities (FERC-510); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-22

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IC11-510-000] Commission Information Collection Activities (FERC-510); Comment Request; Extension AGENCY: Federal Energy Regulatory...) 273-0873. SUPPLEMENTARY INFORMATION: The information collected under the requirements of FERC-510...

  1. 77 FR 40348 - Commission Information Collection Activities (FERC-546); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-09

    ... information collection, FERC-546, Certificated Filings: Gas Pipeline Rates. DATES: Comments on the collection... (202) 273-0873. SUPPLEMENTARY INFORMATION: Title: FERC-546, Certificated Filings: Gas Pipeline Rates... transportation, natural gas storage, and unbundled sales activities of interstate natural gas pipelines and...

  2. 75 FR 64301 - Commission Information Collection Activities (FERC-537); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-19

    ... Information Collection Activities (FERC-537); Comment Request; Extension October 8, 2010. AGENCY: Federal Energy Regulatory Commission. ACTION: Notice of proposed information collection and request for comments... (Commission or FERC) is soliciting public comment on the proposed information collection described below...

  3. 77 FR 56636 - Commission Information Collection Activities (FERC-500 & FERC-505); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ...) to provide the Commission with the responsibility of issuing licenses for non-federal hydroelectric... States public. Types of Respondents: Non-federal hydroelectric plants greater than 5 megawatt capacity (FERC-500); non-federal hydroelectric plants less than 5 megawatts capacity (FERC-505). Estimate of...

  4. 76 FR 34689 - Commission Information Collection Activities [FERC-583], Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-14

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IC11-583-000] Commission Information Collection Activities [FERC-583], Comment Request; Extension AGENCY: Federal Energy Regulatory.... 3506(c)(2)(A) (2006), (Pub. L. 104-13), the Federal Energy Regulatory Commission (Commission or FERC...

  5. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions. (a...

  6. 18 CFR 260.401 - FERC Form No. 552, Annual Report of Natural Gas Transactions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual Report of Natural Gas Transactions. 260.401 Section 260.401 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.401 FERC Form No. 552, Annual Report of Natural Gas Transactions. (a...

  7. 78 FR 24188 - Commission Information Collection Activities (FERC-500 and FERC-505); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... information are due by May 9, 2013. ADDRESSES: Comments filed with OMB, identified by the OMB Control Nos... Projects with 5 Megawatt or Less Capacity. OMB Control Nos.: FERC-500 (1902-0058); FERC-505 (1902-0114... Power Act to provide the Commission with the responsibility of issuing licenses for non-federal...

  8. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies. (a...

  9. 18 CFR 260.1 - FERC Form No. 2, Annual report for Major natural gas companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... report for Major natural gas companies. 260.1 Section 260.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.1 FERC Form No. 2, Annual report for Major natural gas companies. (a...

  10. 77 FR 77067 - Commission Information Collection Activities (FERC Form No. 556); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ... Regulatory Policies Act of 1978 (PURPA).\\1\\ FERC is authorized to encourage cogeneration and small power... power production facilities which use other wastes and renewable resources. PURPA, encourages the... order to obtain qualifying status; The PURPA benefits which are available to QFs to encourage small...

  11. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural gas...

  12. 18 CFR 260.2 - FERC Form No. 2-A, Annual report for Nonmajor natural gas companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual report for Nonmajor natural gas companies. 260.2 Section 260.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.2 FERC Form No. 2-A, Annual report for Nonmajor natural gas...

  13. 75 FR 5063 - Commission Information Collection Activities (FERC-729); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Information Collection Activities (FERC-729); Comment Request; Submitted for OMB Review January 22, 2010... Regulatory Commission (Commission or FERC) has submitted the information collection described below to the Office of Management and Budget (OMB) for review of the information collection requirements. Any...

  14. 76 FR 4888 - Commission Information Collection Activities (FERC-537); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-27

    ... Information Collection Activities (FERC-537); Comment Request; Submitted for OMB Review January 20, 2011... Regulatory Commission (Commission or FERC) has submitted the information collection described below to the Office of Management and Budget (OMB) for review of the information collection requirements. Any...

  15. 76 FR 64932 - Commission Information Collection Activities (FERC-510); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-19

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IC11-510-001] Commission Information Collection Activities (FERC-510); Comment Request; Submitted for OMB Review AGENCY: Federal Energy... received no comments on the FERC-510 and has made this notation in its submission to OMB. DATES: Comments...

  16. 78 FR 29359 - Commission Information Collection Activities (Ferc-604); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-20

    ... Regulatory Commission (Commission or FERC) is soliciting public comment on FERC- 604, Cash Management... fax at (202) 273-0873. SUPPLEMENTARY INFORMATION: Title: FERC-604, Cash Management Agreements. OMB... requirements. Abstract: Cash management or ``money pool'' programs typically concentrate affiliates' cash...

  17. New FERC chairman says plenty of activity yet to come

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Share, J.

    Utility executives may have slowed their merger and acquisition activity to catch their breath, but it`s far from over, says Jim Hoecker, the new chairman of the Federal Energy Regulatory Commission. He doesn`t think we`ve seen the last of this phenomenon. As the industry begins to understand the shape of market developments to come in the future, increasingly creative M and A activities will be seen. But there`s also many other contractual arrangements among utilities and between utilities, pipelines, and gas distribution companies that reflect the more dynamic market of today. In the interview, he referred to a survey ofmore » utility executives in which as many as 45% indicated that their companies were involved in merger or acquisition activity. That survey found about 70% of these executives felt there is going to be more consolidation within the utility industry and an even larger proportion concluded there would be increased mergers between the electric and natural gas industries. In addition, Hoecker discusses gas versus electric, gas versus coal, and FERC`s future.« less

  18. 77 FR 6550 - Commission Information Collection Activities (FERC-725A); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-08

    ... submitting the information collection FERC-725A, Mandatory Reliability Standards for the Bulk- Power System... Reliability Standard, in an order published in the Federal Register (76 FR 72197, 11/22/2011). FERC received...) 273-0873. SUPPLEMENTARY INFORMATION: Title: FERC-725A, Mandatory Reliability Standards for the Bulk...

  19. 18 CFR 260.300 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 260.300 Section 260... ENERGY APPROVED FORMS, NATURAL GAS ACT STATEMENTS AND REPORTS (SCHEDULES) § 260.300 FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas companies. (a) Prescription...

  20. 78 FR 13657 - Commission Information Collection Activities (FERC-577); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IC13-12-000] Commission Information Collection Activities (FERC-577); Comment Request; Extension AGENCY: Federal Energy Regulatory...: In compliance with the requirements of the Paperwork Reduction Act of 1995, the Federal Energy...

  1. 77 FR 31342 - Commission Information Collection Activities (FERC-914); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-25

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IC12-14-000] Commission Information Collection Activities (FERC-914); Comment Request; Extension AGENCY: Federal Energy Regulatory Commission, Department of Energy. ACTION: Notice of information collection and request for comments. SUMMARY...

  2. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric utilities...

  3. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric utilities...

  4. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric utilities...

  5. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric utilities...

  6. 18 CFR 141.51 - FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Annual Electric Balancing Authority Area and Planning Area Report. 141.51 Section 141.51 Conservation of...) § 141.51 FERC Form No. 714, Annual Electric Balancing Authority Area and Planning Area Report. (a) Who... Policies Act, 16 U.S.C. 2602, operating a balancing authority area, and any group of electric utilities...

  7. 77 FR 70746 - Commission Information Collection Activities (FERC-592); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-27

    ... information collection, Standards of Conduct for Transmission Provider; and Marketing Affiliates of Interstate... transportation information, which allows them to compete with marketing affiliates on a more equal basis. 18 CFR... Information Collection Activities (FERC-592); Comment Request; Extension AGENCY: Federal Energy Regulatory...

  8. 18 CFR 141.400 - FERC Form No. 3-Q, Quarterly financial report of electric utilities, licensees, and natural gas...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Quarterly financial report of electric utilities, licensees, and natural gas companies. 141.400 Section 141..., licensees, and natural gas companies. (a) Prescription. The quarterly report of electric utilities, licensees, and natural gas companies, designated as FERC Form No. 3-Q, is prescribed for the reporting...

  9. 77 FR 58365 - Commission Information Collection Activities (FERC-912); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-20

    ... collection, FERC-912, Cogeneration and Small Power Production, PURPA Section 210(m) Regulations for...-0873. SUPPLEMENTARY INFORMATION: Title: FERC-912, Cogeneration and Small Power Production, PURPA... the Public Utility Regulatory Policies Act of 1978 (PURPA) by adding subsection ``(m)'' that provides...

  10. 77 FR 2963 - Commission Information Collection Activities (FERC-725F); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... submitting the information collection FERC-725F, ``Mandatory Reliability Standards for Nuclear Plant...: Title: FERC-725F Mandatory Reliability Standards for Nuclear Plant Interface Coordination OMB Control No... added a new section 215 to the FPA, which required a Commission-certified Electric Reliability...

  11. 77 FR 97 - Commission Information Collection Activities, Proposed Collection (FERC-598); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... requirements; (4) training personnel to respond to a collection of information; (5) searching data sources; (6... Information Collection Activities, Proposed Collection (FERC-598); Comment Request; Extension AGENCY: Federal Energy Regulatory Commission. ACTION: Notice of Proposed Information Collection and Request for Comments...

  12. 76 FR 28760 - Commission Information Collection Activities (FERC-73); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-18

    ... collection of data through the FERC Form No. 73. Their support stems from reliance on this data collection...) Reviewing instructions; (2) developing, acquiring, installing, and utilizing technology and systems for the... to providing this information, such as administrative costs and the cost for information technology...

  13. 75 FR 49914 - Commission Information Collection Activities (FERC-511 and FERC-515); Comment Request; Extensions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-16

    ... information. \\5\\ Estimated number of hours an employee works each year = 2080, estimated average annual cost..., along, from, or in any of the streams or other bodies of water over which Congress has jurisdiction... water or water power from any Government dam. FERC-515: The information collected under the requirements...

  14. 76 FR 54749 - Commission Information Collection Activities (FERC-523); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-02

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. IC11-523-001] Commission Information Collection Activities (FERC-523); Comment Request; Submitted for OMB Review AGENCY: Federal Energy Regulatory Commission, Energy. ACTION: Notice. SUMMARY: In compliance with the requirements of section 3507...

  15. Toward an SEC-FERC memorandum of understanding. [SEC-FERC (Securities and Exchange Commission-Federal Energy Regulatory Commission)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Moeller, J.W.

    1994-01-01

    The Securities and Exchange Commission (SEC) is under fire. For the past year, Congress has criticized its administration of the Public Utility Holding Company Act of 1935 (PUHCA). The criticism might persuade Congress to transfer the administration of PUHCA from the SEC to the Federal Energy Regulatory Commission (FERC). Section I of this article briefly describes the events of the past year and the implications of transfer of PUHCA from the SEC to the FERC. Section II of this article discusses the background relationship of PUHCA, under which the SEC regulates the securities transactions of public utilities, to the Federalmore » Power Act, under which the FERC regulates the rates of public utilities. Section III discusses the case involving the Ohio Power Company, which resulted in a November 1990 decision of the Supreme Court, that recently has highlighted the conflict in jurisdiction between the SEC and the FERC relative to the regulation of public utilities. Section IV discusses the immediate responses to that conflict--a proposed amendment to a FERC regulation and S. 544--and the reasons that neither response is an ideal solution. Section V proposes an alternative to those immediate responses--the Memorandum of Understanding. An Appendix is provided which proposes a draft Memorandum of Understanding between the SEC and the FERC.« less

  16. 78 FR 53742 - Commission Information Collection Activities (Ferc-582); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-30

    ... commerce. Respondents (public utilities, power marketers) subject to these annual charges must submit FERC.... Type of Respondents: Public utilities and power marketers. Estimate of Annual Burden: \\8\\ The...

  17. 75 FR 61455 - Commission Information Collection Activities (FERC-582); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... electronically, access the Commission's website and click on Documents & Filing, E-Filing ( http://www.ferc.gov... and power marketers then must pay the charges within 45 days of the Commission's issuance of the bill... a waiver or exemption of the obligation to pay a fee or an annual charge. Public Comment and FERC...

  18. 76 FR 49460 - Commission Information Collection Activities (FERC 516A); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-10

    ... Information Collection Activities (FERC 516A); Comment Request; Submitted for OMB Review AGENCY: Federal... Office of Management and Budget, Office of Information and Regulatory Affairs, Attention: Federal Energy... estimated total cost to respondents is $84,739 (rounded) [1,238 hours divided by 2,080 hours per year, times...

  19. 77 FR 39691 - Commission Information Collection Activities (FERC-725); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... information collection FERC-725, Certification of Electric Reliability Organization; Procedures for Electric Reliability Standards, to the Office of Management and Budget (OMB) for review of the information collection..., Certification of Electric Reliability Organization; Procedures for Electric Reliability Standards. OMB Control...

  20. 78 FR 58296 - Commission Information Collection Activities (FERC-604); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-23

    ... collection requirements with no changes to the reporting requirements. Abstract: Cash management or ``money... unregulated) pools of money in cash management programs could detrimentally affect regulated rates. To protect... submitting the information collection FERC-604 (Cash Management Agreements) to the Office of Management and...

  1. 78 FR 16487 - Commission Information Collection Activities (FERC-556); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... Policies Act of 1978 (PURPA).\\1\\ FERC is authorized to encourage cogeneration and small power production... power production facilities which use other wastes and renewable resources. PURPA, encourages the... order to obtain qualifying status; the PURPA benefits which are available to QFs to encourage small...

  2. 77 FR 16214 - Commission Information Collection Activities (FERC-598); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ... Generator or Foreign Utility Company Status, to the Office of Management and Budget (OMB) for review of the...., Washington, DC 20426. Instructions: All submissions must be formatted and filed in accordance with submission...: Title: FERC-598, Self Certification for Entities Seeking Exempt Wholesale Generator or Foreign Utility...

  3. 78 FR 16485 - Commission Information Collection Activities (FERC-607); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... submitting the information collection, FERC-607 (Report on Decision or Action on Request for Federal... and is making this notation in its submittal to OMB. DATES: Comments on the collection of information....gov . Attention: Federal Energy Regulatory Commission Desk Officer. The Desk Officer may also be...

  4. 77 FR 71186 - Commission Information Collection Activities (FERC-912); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-29

    ... submitting the information collection FERC-912, Cogeneration and Small Power Production, PURPA Section 210(m... Production, PURPA Section 210(m) Regulations for Termination or Reinstatement of Obligation to Purchase or... Section 210 of the Public Utility Regulatory Policies Act of 1978 (PURPA) by adding subsection ``(m...

  5. 77 FR 64495 - Commission Information Collection Activities (FERC-922); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-22

    ... submitting the new information collection FERC-922, ``Performance Metrics in Regions Outside ISOs and RTOs... RTOs. OMB Control No.: To be determined. Type of Request: Approval of a new information collection. Abstract: The collection of information requires those public utilities outside of ISOs and RTOs that...

  6. 78 FR 63172 - Commission Information Collection Activities (FERC-733); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-23

    ... Electric Reliability Corporation (NERC) and EIA, to fielding and collecting data using a FERC designed... Commission in time to complete the 2014 report to Congress. NERC, as the Electric Reliability Organization... dispatchable and non-dispatchable resources that it needs to conduct its reliability work. Reporting demand...

  7. 78 FR 11167 - Commission Information Collection Activities (FERC-592); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-15

    ... Marketing Affiliates of Interstate Pipelines), to the Office of Management and Budget (OMB) for review of... user assistance contact FERC Online Support by email at [email protected] , or by phone at...; and Marketing Affiliates of Interstate Pipelines). OMB Control No.: 1902-0157. Type of Request: Three...

  8. 78 FR 54880 - Commission Information Collection Activities (FERC-551); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-06

    ... making this notation in its submittal to OMB. DATES: Comments on the collection of information are due by... . Attention: Federal Energy Regulatory Commission Desk Officer. The Desk Officer may also be reached via...: Three-year extension of the FERC-551 information collection requirements with no changes to the...

  9. 77 FR 28861 - Commission Information Collection Activities (FERC-585); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ... Energy shortages and Contingency Plans under PURPA, to the Office of Management and Budget (OMB) for... Contingency Plans under PURPA. OMB Control No.: 1902-0138. Type of Request: Three-year extension of the FERC... Contingency Plans under PURPA'', is used by the Commission to implement the statutory provisions of section...

  10. 76 FR 18743 - Commission Information Collection Activities (FERC-603); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-05

    ... FERC-603 ``Critical Energy Infrastructure Information'' (OMB No. 1902-0197) is used by the Commission to implement procedures for gaining access to critical energy infrastructure information (CEII) that... information about ``existing or proposed critical infrastructure that (i) relates to the production...

  11. 77 FR 16215 - Commission Information Collection Activities (FERC-716); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ...: Title: FERC-716, Good Faith Request for Transmission Service and Response by Transmitting Utility Under... valid good faith request for transmission has been made under section 211 of the FPA. By developing the.... As a result, 18 CFR 2.20 identifies 12 components of a good faith estimate and 5 components of a...

  12. 77 FR 53877 - Commission Information Collection Activities (FERC-715); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    ...; A detailed description of the transmission planning reliability criteria used to evaluate system... reliability criteria are applied and the steps taken in performing transmission planning studies); and A... reliability criteria using its stated assessment practices. The FERC-715 enables the Commission to use the...

  13. 78 FR 49490 - Commission Information Collection Activities (Ferc-511); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-14

    ... information collection, FERC-511 (Transfer of Electric License). DATES: Comments on the collection of...-0873. SUPPLEMENTARY INFORMATION: Title: Transfer of Electric License. OMB Control No.: 1902-0069... utilization of power.\\2\\ Section 8 of the FPA provides that the voluntary transfer of any license is made only...

  14. 78 FR 58295 - Commission Information Collection Activities (FERC-725A); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-23

    ... submitting the information collection FERC-725A, Mandatory Reliability Standards for the Bulk Power System... collection analysis associated with its approval of Reliability Standard EOP-004-2, in an order published in... solicitation and is making this notation in its submission to OMB. \\1\\ North American Electric Reliability Corp...

  15. 77 FR 28863 - Commission Information Collection Activities (FERC-915); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ... under section 205 of the Federal Power Act (FPA) to ensure that wholesale sales of electricity are just... maintained by the respondents under FERC-915 to monitor the entities' sales, ensure that the prices are just and reasonable, maintain the integrity of the wholesale jurisdictional sales markets, and ensure that...

  16. FERC examines transmission access pricing policies

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Burkhart, L.A.

    1993-02-15

    The Federal Energy Regulatory Commission (FERC) has approved two orders dealing with transmission pricing issues that evolved from its March 1992 Pennsylvania Electric Co. (Penelec) decision dealing with cost recovery of transmission plant expansion. Commissioner Betsy Moler said the cases represented the first time the FERC has used incremental rates for wheeling transactions. In the first new order, Public Service Electric Gas Co. proposed charging for third-party transmission services to recover the sum of its standard embedded cost rate and the net incremental cost of making upgrades to its integrated transmission system. Public Service sought to provide service to EEAmore » I Limited, which proposed building and operating a cogeneration facility at a United States Gypsum Co. plant in New Jersey. Consolidated Edison Company of New York Inc. had agreed to purchase the project's output. In rejecting Public Service's proposal, the FERC ruled the utility must charge a transmission rate that is the higher of either its embedded cost rate or its incremental cost rate of accelerated expansion. (In this instance, the incremental rate was higher). In the second new order, Public Service Co. of Colorado (PSCC) filed a proposed open access transmission service tariff related to its acquisition of facilities from Colorado-Ute Electric Association Inc. The FERC rejected PSCC's tariff request that would have required a new transmission customer (in the event PSCC modified its integrated transmission grid) to pay the sum of PSCC's standard transmission rate (reflecting the average cost of the grid facilities) plus the expansion cost (reflecting the incremental facility cost).« less

  17. 18 CFR 366.4 - FERC-65, notification of holding company status, FERC-65A, exemption notification, and FERC-65B...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false FERC-65, notification... Section 366.4 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT... NATURAL GAS ACT BOOKS AND RECORDS Definitions and Provisions Under PUHCA 2005, the Federal Power Act and...

  18. 18 CFR 366.4 - FERC-65, notification of holding company status, FERC-65A, exemption notification, and FERC-65B...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false FERC-65, notification... Section 366.4 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT... NATURAL GAS ACT BOOKS AND RECORDS Definitions and Provisions Under PUHCA 2005, the Federal Power Act and...

  19. FERC ruling highlights differences between notification and certification procedures for qualifying facilities

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1984-06-01

    On June 15, the FERC dismissed a motion by the Puerto Rico Electric Power Authority (PREPA) to intervene to challenge the filing of Merck, Sharp and Dohme Quimica De Puerto Rico, Inc. (Merck), for qualifying status under PURPA. (Docket QF 84-188). The FERC's reason for dismissing the motion for intervention was that the Merck submission was not a true application for certification, but rather, merely a notice that the proposed facility would in fact be a qualifying facility. Under FERC rules, a qualifying cogenerator or small power producer may follow either of two procedures: (1) an application procedure, under whichmore » the FERC will rule on whether a particular facility is a qualifying facility, or (2) a notification procedure, under which FERC does not rule. The application is a voluntary, optional procedure, provided by FERC for situations in which a potential qualifying facility requires affirmative certification, typically for financing purposes. Otherwise, merely filing a notification is sufficient for a qualifying facility to be able to take advantage of the benefits of PURPA.« less

  20. 76 FR 54748 - Commission Information Collection Activities (FERC-519); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-02

    ... . All comments may be viewed, printed or downloaded remotely via the Internet through FERC's homepage using the ``eLibrary'' link. For user assistance, contact [email protected] or toll-free at...

  1. 77 FR 98 - Commission Information Collection Activities, Proposed Collection (FERC-716); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... requirements of FERC-716 (``Good Faith Request for Transmission Service and Response by Transmitting Utility..., provide standards by which the Commission determines if and when a valid good faith request for... 12 components of a good faith estimate and 5 components of a reply to a good faith request. Action...

  2. Implications of the Bangor Hydro decision on FERC relicensings

    USGS Publications Warehouse

    Pizzi, L.

    1997-01-01

    In the next 15 years, over 400 existing nonfederal dams will require new licenses by the Federal Energy Regulatory Commission (FERC) in order to continue operations. In many cases, state or federal agencies have a unique opportunity to condition the hydropower licenses to protect environmental values. In 1996, the Court of Appeals for the District of Columbia Circuit issued an opinion striking down a mandatory license condition imposed by the U.S. Department of the Interior in a FERC relicensing. The case, Bangor Hydro-Electric Company v.Federal Energy Regulatory Commission, is instructive for agencies regarding the procedures to be followed in imposing mandatory FERC licensing conditions in the future. After Bangor Hydro, agencies should: (1) support their decisions by substantial evidence in the FERC record, (2) request applicant studies to support agency decisions and allow public comment on the decisions as a means to test the facts and analysis, and (3) consider intervening in a case to defend the condition on appeal. Bangor Hydro also raises, but does not decide, the issue of whether the agency imposing the mandatory condition should engage in balancing the economics of the hydropower project with the public resources affected by the project.

  3. 30 CFR 285.638 - What must I do upon completion of my commercial operations as approved in my COP or FERC license?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... operations as approved in my COP or FERC license? 285.638 Section 285.638 Mineral Resources BUREAU OF OCEAN... Activities Under An Approved Cop § 285.638 What must I do upon completion of my commercial operations as approved in my COP or FERC license? (a) Upon completion of your approved activities under your COP, you...

  4. 76 FR 35872 - Commission Information Collection Activities (Ferc-603); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    .... Critical infrastructure means existing and proposed systems and assets, whether physical or virtual, the....ferc.gov/help/submission-guide.asp . To file the document electronically, access the Commission's Web... using the ``eLibrary'' link. For user assistance, contact [email protected] or toll-free at...

  5. 30 CFR 285.638 - What must I do upon completion of my commercial operations as approved in my COP or FERC license?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... operations as approved in my COP or FERC license? 285.638 Section 285.638 Mineral Resources MINERALS... FACILITIES ON THE OUTER CONTINENTAL SHELF Plans and Information Requirements Activities Under An Approved Cop § 285.638 What must I do upon completion of my commercial operations as approved in my COP or FERC...

  6. 77 FR 20383 - Notice of Revisions to FERC Online, eSubscription Service

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-04

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Revisions to FERC Online, eSubscription Service Take notice that on March 30, 2012, the Federal Energy Regulatory Commission (Commission...Library in a subscribed-to docket. eSubscription is one of the Commission's ``FERC Online'' services...

  7. 30 CFR 585.638 - What must I do upon completion of my commercial operations as approved in my COP or FERC license?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... operations as approved in my COP or FERC license? 585.638 Section 585.638 Mineral Resources BUREAU OF OCEAN... FACILITIES ON THE OUTER CONTINENTAL SHELF Plans and Information Requirements Activities Under An Approved Cop § 585.638 What must I do upon completion of my commercial operations as approved in my COP or FERC...

  8. 30 CFR 585.638 - What must I do upon completion of my commercial operations as approved in my COP or FERC license?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... operations as approved in my COP or FERC license? 585.638 Section 585.638 Mineral Resources BUREAU OF OCEAN... FACILITIES ON THE OUTER CONTINENTAL SHELF Plans and Information Requirements Activities Under An Approved Cop § 585.638 What must I do upon completion of my commercial operations as approved in my COP or FERC...

  9. 30 CFR 585.638 - What must I do upon completion of my commercial operations as approved in my COP or FERC license?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... operations as approved in my COP or FERC license? 585.638 Section 585.638 Mineral Resources BUREAU OF OCEAN... FACILITIES ON THE OUTER CONTINENTAL SHELF Plans and Information Requirements Activities Under An Approved Cop § 585.638 What must I do upon completion of my commercial operations as approved in my COP or FERC...

  10. 75 FR 74696 - Commission Information Collection Activities (FERC-555); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-01

    ... Commission as explained below. The Commission issued a Notice in the Federal Register (75 FR 57744, 09/22... applicable records for the regulated public utilities, natural gas and oil pipeline companies subject to the... record retention requirements under FERC-555 were underestimated in the previous renewal of this...

  11. 76 FR 18209 - Commission Information Collection Activities (FERC-725D); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... by the FERC-725D, ``Facilities Design, Connections and Maintenance Reliability Standards'' (OMB... 2005 added a new section 215 to the FPA, which required a Commission-certified Electric Reliability Organization (ERO) to develop mandatory and enforceable reliability standards, which are subject to Commission...

  12. 76 FR 52646 - Commision Information Collection Activities [FERC-588]; Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... 1978 (NGPA), 15 USC 3301- 3432. Under the NGA, a natural gas company must obtain Commission approval to engage in the transportation, sale or exchange of natural gas in interstate commerce. However, Section 7... Commission issued a Notice in the Federal Register (76 FR 30928, 05/27/2011) requesting public comments. FERC...

  13. 76 FR 58803 - Commission Information Collection Activities (FERC-725A); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-22

    ... in Docket No. RD11-4-000 under the requirements of FERC-725A, ``Mandatory Reliability Standards for the Bulk-Power System'' (OMB No. 1902-0244), is required by Commission-approved Reliability Standard EOP-008-1 (Loss of Control Center Functionality) that requires reliability coordinators, transmission...

  14. 76 FR 4516 - Revisions to Forms, Statements, and Reporting Requirements for Natural Gas Pipelines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ...; Order No. 710-B] Revisions to Forms, Statements, and Reporting Requirements for Natural Gas Pipelines... for natural gas companies, contained in FERC Form Nos. 2, 2-A, and 3-Q, to include functionalized fuel..., and reports for natural gas companies, contained in FERC Form Nos. 2, 2-A, and 3-Q, to include...

  15. 77 FR 26537 - Notice of Commissioners and Staff Attendance at FERC Leadership Development Program Graduation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-04

    ... Attendance at FERC Leadership Development Program Graduation/Induction Ceremony The Federal Energy Regulatory... may attend the following event: FERC Leadership Development Program Graduation/Induction Ceremony: 888... and welcome 17 employees selected for the 2012 Leadership Development Program and graduate 15...

  16. 76 FR 5799 - Notice of Commissioners and Staff Attendance at FERC Leadership Development Program Induction...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-02

    ... Attendance at FERC Leadership Development Program Induction Ceremony January 26, 2011. The Federal Energy... Commission staff may attend the following event: FERC Leadership Development Program Induction Ceremony: 888... welcome 16 employees selected for the 2011 Leadership Development Program. Kimberly D. Bose, Secretary...

  17. 76 FR 31320 - Commission Information Collection Activities (FERC-725B); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ... respondent response \\7\\ hours (1) (2) (3) (1) x (2) x (3) FERC-725B: Category 1--Estimate of U.S. 345 1 1,920... 138,720 Entities that have not identified Critical Cyber Assets. Category 3--New U.S. Entities 6 1 3... longer required Category 1: -2...... 1 Category 1 (2 -3,840 to comply with CIP Standards respondents...

  18. 77 FR 69450 - Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-19

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance Docket... gives notice that on November 16, 2012, members of its staff will attend a meeting conducted by... is open to all stakeholders and Commission staff's attendance is part of the Commission's ongoing...

  19. Supreme court agrees: FERC must regulate wholesale markets

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wolak, Frank A.

    The author believes that wholesale markets in the United States would have a greater likelihood of ultimately benefiting consumers if the Federal Energy Regulatory Commission did not have the mandate under the Federal Power Act (FPA) to ensure that wholesale prices are ''just and reasonable.'' However, he continues to believe that the FERC cannot avoid having an ex post criteria for asssessing whether market prices are just and reasonable. Moreover, changes in the design and regulatory oversight of U.S. wholesale electricity markets in recent years, including the recent Supreme Court decision, have caused him to believe even more strongly inmore » the guardrails-for-market-outcomes approach. Finally, several questions are addressed which relate to the pricing of fixed-price, long-term contracts and the impact of these obligations on the behavior of suppliers in short-term wholesale markets that are directly relevant to answering the two major questions that the Supreme Court remanded to FERC in its recent decision.« less

  20. Regional Transmission Planning: A review of practices following FERC Order Nos. 890 and 1000

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Eto, Joseph H.; Gallo, Guilia

    Federal Energy Regulatory Commission (FERC) Order Nos. 890 and 10001 established requirements that transmission planning regions must follow in regional transmission planning and allocating the costs of new transmission facilities. Order No. 890, issued in 2007, outlined general requirements for local as well as regional transmission planning practices and procedures. Order No. 1000, issued in 2011, laid out specific requirements for: (1) regional transmission planning; (2) consideration of transmission needs driven by public policy requirements; (3) non-incumbent transmission development; (4) interregional transmission coordination; and (5) cost allocation for transmission facilities that have been selected in a regional transmission plan formore » purposes of cost allocation. This report reviews how these FERC orders are being implemented by the 12 transmission planning regions recognized by FERC.« less

  1. 75 FR 71123 - Notice of Commissioners and Staff Attendance at FERC/NARUC Collaborative on Smart Response Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of Commissioners and Staff Attendance at FERC/NARUC Collaborative on Smart Response Meeting November 12, 2010. The Federal Energy... Commission staff may attend the following meeting: FERC/NARUC Collaborative on Smart Response: Omni Hotel at...

  2. 75 FR 42747 - Smart Grid Update; Notice of Commissioner and Staff Attendance at FERC/NARUC Collaborative on...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AD10-15-000] Smart Grid Update; Notice of Commissioner and Staff Attendance at FERC/NARUC Collaborative on Smart Response Meeting... Commission and/or Commission staff may attend the following meeting: FERC/NARUC Collaborative on Smart...

  3. Stranded investment, prices and privacy factor in FERC rulings

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    O`Driscoll, M.

    The Federal Energy Regulatory Commission upheld its rejection of United Illuminating Co.`s bid to recover stranded investment costs. Since UI has no wholesale customers, it is a matter best left to state regulators, FERC said. UI`s stranded investment recovery plan was part of the company`s transmission access tariff, which provides for open access transmission service at cost-based rates. FERC ordered UI to delete the stranded investment provisions, saying UI was trying to recover in its wholesale transmission rates the costs of generation facility investments that were incurred to provide service to retail customers that leave its system, reasoning that UImore » was seeking protection from what may be legitimate retail franchise competition, which is a state matter. UI, however, said deleting the stranded investment provision would preclude it from arguing in an individual rate filling under the transmission tariff that stranded investment costs should be borne by the wheeling customer.« less

  4. Conducting a FERC environmental assessment: a case study and recommendations from the Terror Lake Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Olive, S.W.; Lamb, B.L.

    This paper is an account of the process that evolved during acquisition of the license to operate the Terror Lake hydroelectric power project under the auspices of the Federal Energy Regulatory Commission (FERC). The Commission is responsible for granting these licenses under the Federal Power Act (16 U.S.C. 792 et seq.). This act provides, in part, that FERC may condition a license to protect the public interest. The public interest in these cases has come to include both instream and terrestrial values. The Terror River is located on Kodiak Island in Alaska. The river is within the Kodiak National Wildlifemore » Refuge; it supports excellent runs of several species of Pacific Salmon which are both commercially important and a prime source of nutrition for the Kodiak brown bear. The river is also a prime resource for generating electric power. One major concern in the negotiations was the impact of land disturbance and management practices on brown bear habitat - i.e., protection of the brown bear. Maintenance of the bears' habitat is the main purpose of the Kodiak National Wildlife Refuge. But, like many other projects, resolving the instream flow issue was of major importance in the issuance of the FERC license. This paper discusses the fish and wildlife questions, but concentrates on instream uses and how protection of these uses was decided. With this as a focus, the paper explains the FERC process, gives a history of the Terror Lake Project, and, ultimately, makes recommendations for improved management of controversies within the context of the FERC licensing procedures. 65 references.« less

  5. Conducting a FERC environmental assessment: a case study and recommendations from the Terror Lake Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Olive, S.W.; Lamb, B.L.

    This paper is an account of the process that evolved during acquisition of the license to operate the Terror Lake hydro-electric power project under the auspices of the Federal Energy Regulatory Commission (FERC). The Terror River is located on Kodiak Island in Alaska. The river is within the Kodiak National Wildlife Refuge; it supports excellent runs of several species of Pacific Salmon which are both commercially important and a prime source of nutrition for the Kodiak brown bear. This paper discusses both the fish and wildlife questions, but concentrates on instream uses and how protection of these uses was decided.more » In this focus the paper explains the FERC process, gives a history of the Terror Lake Project, and, ultimately, makes recommendations for improved management of controversies within the context of FERC licensing procedures. 64 references.« less

  6. 76 FR 46668 - Revision to Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ...] Revision to Form No. 6 AGENCY: Federal Energy Regulatory Commission. ACTION: Notice of proposed rulemaking... 700 of FERC Form No. 6 (Form 6) to ensure that pipelines report interstate-only barrel and barrel-mile... 700 of their 2010 Form 6 to file a revised page 700 containing only interstate data for the years 2009...

  7. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false System flow diagrams...) § 260.8 System flow diagrams: Format No. FERC 567. (a) Each Major natural gas pipeline company, having a... file with the Commission by June 1 of each year five (5) copies of a diagram or diagrams reflecting...

  8. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false System flow diagrams...) § 260.8 System flow diagrams: Format No. FERC 567. (a) Each Major natural gas pipeline company, having a... file with the Commission by June 1 of each year five (5) copies of a diagram or diagrams reflecting...

  9. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false System flow diagrams...) § 260.8 System flow diagrams: Format No. FERC 567. (a) Each Major natural gas pipeline company, having a... file with the Commission by June 1 of each year five (5) copies of a diagram or diagrams reflecting...

  10. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false System flow diagrams...) § 260.8 System flow diagrams: Format No. FERC 567. (a) Each Major natural gas pipeline company, having a... file with the Commission by June 1 of each year five (5) copies of a diagram or diagrams reflecting...

  11. 18 CFR 260.8 - System flow diagrams: Format No. FERC 567.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false System flow diagrams...) § 260.8 System flow diagrams: Format No. FERC 567. (a) Each Major natural gas pipeline company, having a... file with the Commission by June 1 of each year five (5) copies of a diagram or diagrams reflecting...

  12. 78 FR 6321 - PacifiCorp, et al; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ...Corp, et al; Notice of FERC Staff Attendance PacifiCorp Docket No. ER13-64-000 Deseret Generation... Regulatory Commission (Commission) hereby gives notice that on January 30, 2013, members of its staff will... and Commission staff's attendance is part of the Commission's ongoing outreach efforts. The meeting...

  13. Two-Phased Approach to Synchronize the FERC-USACE Processes for Authorizing Non-Federal Hydropower Projects

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    2016-07-01

    Publication summarizing how the United States Army Corps of Engineers (USACE) and Federal Regulatory Energy Commission (FERC) have developed a two-phased, coordinated approach to regulating non-federal hydropower projects.

  14. 77 FR 41402 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-13

    ...] California Independent System Operator Corporation; Notice of FERC Staff Attendance The Federal Energy Regulatory Commission (Commission) hereby gives notice that on the following date members of its staff will... by the CAISO, the teleconferences and meetings are open to all market participants and staff's...

  15. 76 FR 65191 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-20

    ...; Notice of FERC Staff Attendance The Federal Energy Regulatory Commission (Commission) hereby gives notice that on the following dates members of its staff will participate in teleconferences and meetings to be... staff's attendance is part of the Commission's ongoing outreach efforts. The teleconferences and...

  16. 78 FR 34093 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... Independent System Operator Corporation; Notice of FERC Staff Attendance The Federal Energy Regulatory Commission (Commission) hereby gives notice that on the following date members of its staff will participate... teleconferences and meetings are open to all market participants and staff's attendance is part of the Commission...

  17. 76 FR 76157 - California Independent System, Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-06

    ..., Operator Corporation; Notice of FERC Staff Attendance The Federal Energy Regulatory Commission (Commission) hereby gives notice that on the following dates members of its staff will participate in teleconferences... teleconferences and meetings are open to all market participants, and staff's attendance is part of the Commission...

  18. 76 FR 55380 - California Independent System Operator Corporation; Notice of FERC Staff Attendance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ...; Notice of FERC Staff Attendance The Federal Energy Regulatory Commission (Commission) hereby gives notice that on the following dates members of its staff will participate in teleconferences and meetings to be.... Sponsored by the CAISO, the teleconferences and meetings are open to all market participants, and staff's...

  19. 75 FR 61365 - Revisions to Forms, Statements, and Reporting Requirements for Natural Gas Pipelines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... proposing to revise certain financial reporting forms required to be filed by natural gas companies (FERC... the above-referenced proceeding \\1\\ proposing to revise certain financial reporting forms required by...] Revisions to Forms, Statements, and Reporting Requirements for Natural Gas Pipelines September 24, 2010...

  20. 76 FR 28024 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meetings noted below. Their attendance is part of the Commission's...

  1. 77 FR 50683 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its staff may attend the meeting noted below. Their attendance is part of...

  2. 78 FR 11868 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-20

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Meeting The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its staff may attend the meeting noted below. Their attendance is part of...

  3. 77 FR 58829 - Notice of FERC Staff Attendance at Entergy/Cleco Teleconference on Order No. 1000

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at Entergy/Cleco Teleconference on Order No. 1000 The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its staff may participate in the teleconference noted below. Their...

  4. 75 FR 47302 - Notice of FERC Attendance at the Entergy Regional State Committee Meeting, Entergy ICT...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-05

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Attendance at the Entergy Regional State Committee Meeting, Entergy ICT Tranmission Planning Summit and Entergy SPP RTO Planning... Orleans, 500 Canal Street, New Orleans, LA 70130, 504-525-2500. Entergy ICT Transmission Planning Summit...

  5. 77 FR 29634 - Notice of FERC Staff Attendance at the Louisiana Public Service Commission's Business and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Louisiana Public Service Commission's Business and Executive Session Meeting The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meeting noted below. Their attendance...

  6. 76 FR 62804 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and Stakeholder Meeting The Federal Energy Regulatory... is part of the Commission's ongoing outreach efforts. Entergy Regional State Committee Work Group and...

  7. 77 FR 21979 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Working Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-12

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Working Group and Stakeholder Meeting The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its staff may attend the meeting noted below...

  8. 77 FR 47630 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Working Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-09

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Working Group and Stakeholder Meeting The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its staff may attend the meeting noted below...

  9. 76 FR 67727 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-02

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and Stakeholder Meeting The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meeting noted below. Their attendance...

  10. 77 FR 28872 - Notice of FERC Staff Attendance at the SPP-ITO Louisville Gas & Electric/Kentucky Utilities...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-16

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the SPP-ITO Louisville Gas & Electric/Kentucky Utilities Stakeholder Meeting The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meeting noted below. Their attendance...

  11. 76 FR 49764 - Notice of FERC Staff Attendance at the Entergy ICT Transmission Planning Summit and Entegry...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-11

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy ICT Transmission Planning Summit and Entegry Regional State Committee Meeting The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meetings noted below...

  12. 76 FR 54766 - Notice of FERC Staff Attendance at a Hearing Before the Arkansas Public Service Commission

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-02

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at a Hearing Before the Arkansas Public Service Commission The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the hearing noted below. Their attendance is part of...

  13. 77 FR 3765 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and Stakeholder Meeting The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meeting noted below. Their attendance...

  14. 77 FR 15097 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Working Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-14

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Working Group and Stakeholders Meeting The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meeting noted below. Their attendance...

  15. A guide to FERC regulation and ratemaking of electric utilites and other power suppliers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Small, M.E.

    1994-12-31

    This book is very useful for its thorough survey of issues and cases pertinent to electric utility rate filings at the Federal Energy Regulatory Commission (FERC). As the author states, the book provides a step-by-step analysis of the statutory provisions, regulations and case law that are pertinent to the establishment of electric utility sales and transmission rates. The book takes a nuts and bolts approach to the examination of the ratemaking process for electric utilities. Three chapters cover in detail cost of service, rate of return, functionalization, classification and allocation, and rate design, issues which are at the heart ofmore » traditional cost of service ratemaking. Other chapters explain Title II of the Federal POwer Act and general rate filing procedures. Chapters 7 and 8 address the fuel adjustment clause and price squeeze, respectively. Chapters 9, 10, and 11 largely cover new developments in FERC policies since the previous edition of the book was published in 1989, including market based rates, QF certification issues and transmission issues. This volume is useful to the neophyte to electric utility regulation because of its comprehensiveness in addressing ratemaking issues. Also, it is useful to the seasoned practitioner because of its identification of pertinent case law.« less

  16. 76 FR 13613 - Notice of FERC Staff Attendance at the ICT Stakeholders Policy Committee and Entergy Regional...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the ICT Stakeholders Policy Committee and Entergy Regional State Committee Meetings The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its staff may attend the meetings noted below. Their attendance is part of the...

  17. 75 FR 23262 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-03

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting and the Entergy Regional State Committee Meeting April 26, 2010. The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meetings noted below....

  18. 75 FR 63168 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting and the Entergy Regional State Committee Meeting October 6, 2010. The Federal Energy Regulatory Commission (Commission) hereby gives notice that members of its staff may attend the meeting...

  19. 75 FR 41858 - Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Southwest Power Pool ICT Stakeholder Policy Committee Meeting July 12, 2010. The Federal Energy Regulatory Commission hereby gives notice that members of its staff may attend the meeting noted below. Their attendance is part of the Commission's...

  20. 43 CFR 45.11 - What are the form and content requirements for documents under this subpart?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false What are the form and content requirements for documents under this subpart? 45.11 Section 45.11 Public Lands: Interior Office of the Secretary of the Interior CONDITIONS AND PRESCRIPTIONS IN FERC HYDROPOWER LICENSES Hearing Process Document...

  1. 75 FR 5066 - Commission Information Collection Activities (FERC Form 60,1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... corresponding dockets and collection numbers.) Comments may be filed either electronically or in paper format. Those persons filing electronically do not need to make a paper filing. Documents filed electronically... acknowledgement to the sender's e- mail address upon receipt of comments. For paper filings, the comments should...

  2. Are state renewable feed-in tariff initiatives truly throttled by Federal statutes after the FERC California decision?

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Yaffe, David P.

    For the last few years, several local and state governments have adopted ''feed-in tariffs'' to promote development of dispersed, small-scale renewable generation through incentive pricing. Most FITs are intended to stimulate development of small solar or renewable energy facilities. In July, FERC issued a decision restating that the Federal Power Act and PURPA 210, not state (or local) legislation, govern the price that local utilities may pay under FITs. (author)

  3. 75 FR 15424 - Commission Information Collection Activities (FERC-538, FERC-539, FERC-577, FERC-606, and FERC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... Natural Gas Act (NGA) (Pub. L. 75-688) (15 U.S.C. 717-717w), upon application by a local distribution... the Commission to direct the natural gas pipeline company to render (such as a request to direct a...-539. Section 3 of the Natural Gas Act (NGA) (Pub. L. 75-688) (15 U.S.C. 717-717w) provides, in part...

  4. Assessing the "need to negotiate" in FERC licensing consultations: a study of two hydropower projects

    USGS Publications Warehouse

    Coughlan, Beth A. K.; Burkardt, Nina; Fulton, David

    1993-01-01

    We investigated the “need to negotiate” in a comparative case study of multi-agency negotiations in the FERC licensing process. Researchers interviewed participants in two cases involving environmental consultations and asked about parties' level of need to negotiate throughout the process. Participants identified a need to negotiate, and when this need was strongly felt, there was an increased opportunity for an agreement to be reached. An intense need to negotiate by all parties is not a prerequisite to successful agreements. When key participants have a strong need to negotiate, they can instigate negotiations and encourage the involvement of other parties.

  5. 7 CFR 1.611 - What are the form and content requirements for documents under §§ 1.610 through 1.660?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false What are the form and content requirements for documents under §§ 1.610 through 1.660? 1.611 Section 1.611 Agriculture Office of the Secretary of Agriculture ADMINISTRATIVE REGULATIONS Conditions in FERC Hydropower Licenses Document Filing and Service § 1...

  6. 78 FR 34373 - Flexible and Local Resources Needed for Reliability in the California Wholesale Electric Market...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-07

    ... form at: https://www.ferc.gov/whats-new/registration/elec-markets-07-31-13-speaker-form.asp . Due to....ferc.gov/whats-new/registration/elec-markets-07-31-13-form.asp . The technical conference will not be...

  7. 76 FR 34217 - Commission Information Collection Activities, Proposed Collections; Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-13

    ... of Information and Regulatory Affairs, Attention: Federal Energy Regulatory Commission Desk Officer... and omissions of the Act's provisions occur. The FERC-520 is divided into two types of applications... and shares its interest in identifying and implementing burden reductions to the benefit of form...

  8. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... form. (d) Critical Energy Infrastructure Information. (1) If the instructions in Form No. 715 require a utility to reveal Critical Energy Infrastructure Information (CEII), as defined in § 388.113(c) of this... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to...

  9. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... form. (d) Critical Energy Infrastructure Information. (1) If the instructions in Form No. 715 require a utility to reveal Critical Energy Infrastructure Information (CEII), as defined in § 388.113(c) of this... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to...

  10. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... form. (d) Critical Energy Infrastructure Information. (1) If the instructions in Form No. 715 require a utility to reveal Critical Energy Infrastructure Information (CEII), as defined in § 388.113(c) of this... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to...

  11. 18 CFR 141.300 - FERC Form No. 715, Annual Transmission Planning and Evaluation Report.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... form. (d) Critical Energy Infrastructure Information. (1) If the instructions in Form No. 715 require a utility to reveal Critical Energy Infrastructure Information (CEII), as defined in § 388.113(c) of this... Critical Energy Infrastructure Information (CEII) may be found at 18 CFR 388.113. Requests for access to...

  12. 75 FR 19630 - Commission Information Collection Activities (FERC Form 80); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ..., instructions, and glossary remain unchanged and are not attached. Action: The Commission is requesting a three... of terms, please refer to the Glossary. c. Report actual data for each item. If actual data are..., instructions, and glossary) are unchanged and are not included here.] [FR Doc. 2010-8584 Filed 4-14-10; 8:45 am...

  13. 78 FR 24187 - Commission Information Collection Activities (FERC Form 6-Q); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... necessary or appropriate. Financial accounting and reporting provides necessary information concerning a... entities within the energy industry. It also provides timely disclosures of the impacts that new accounting... jurisdictional entities assists the Commission in its analysis of profitability, efficiency, risk, and in its...

  14. 76 FR 17413 - Contract Reporting Requirements of Intrastate Natural Gas Companies; Notice of Technical Workshop...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-29

    ... the PDF or XML version of Form No. 549D as a method to eFile quarterly data and whether they intend on... fillable Form No. 549D PDF and XML to file data \\2\\ pursuant to Order Nos. 735 and 735-A.\\3\\ [[Page 17414... PDF ( http://www.ferc.gov/docs-filing/forms/form-549d/form-549d.pdf ) or XML ( http://www.ferc.gov...

  15. 30 CFR 585.618 - What must I do upon completion of approved site assessment activities?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... assessment term, you timely submit a COP meeting the requirements of this subpart, or a complete FERC license... facilities in place on your lease during the time that BOEM reviews your COP for approval or FERC reviews... for facilities that are authorized to remain in place under your approved COP or approved FERC license...

  16. 30 CFR 585.618 - What must I do upon completion of approved site assessment activities?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... assessment term, you timely submit a COP meeting the requirements of this subpart, or a complete FERC license... facilities in place on your lease during the time that BOEM reviews your COP for approval or FERC reviews... for facilities that are authorized to remain in place under your approved COP or approved FERC license...

  17. 30 CFR 585.618 - What must I do upon completion of approved site assessment activities?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... assessment term, you timely submit a COP meeting the requirements of this subpart, or a complete FERC license... facilities in place on your lease during the time that BOEM reviews your COP for approval or FERC reviews... for facilities that are authorized to remain in place under your approved COP or approved FERC license...

  18. 78 FR 8500 - Commission Information Collection Activities (FERC Form 6-Q); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... necessary or appropriate. Financial accounting and reporting provides necessary information concerning a... entities within the energy industry. It also provides timely disclosures of the impacts that new accounting... jurisdictional entities assists the Commission in its analysis of profitability, efficiency, risk and in its...

  19. 75 FR 4550 - Black Marlin Pipeline Company; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-28

    ... public accountant (CPA) certification statement for the FERC Form No. 2-A for 2009. Any person desiring...://www.ferc.gov , using the ``eLibrary'' link and is available for review in the Commission's Public...

  20. 78 FR 16843 - Commission Information Collection Activities; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-19

    ... Section 219 \\1\\ of the Federal Power Act, the Commission issued FERC Order No. 679 \\2\\, Promoting Transmission Investment Through Pricing Reform. In Order No. 679 FERC amended its regulations in 18 CFR 35.35... consumers by ensuring reliability and to reduce the cost of delivered power by reducing transmission...

  1. Pore-forming activity of clostridial binary toxins.

    PubMed

    Knapp, O; Benz, R; Popoff, M R

    2016-03-01

    Clostridial binary toxins (Clostridium perfringens Iota toxin, Clostridium difficile transferase, Clostridium spiroforme toxin, Clostridium botulinum C2 toxin) as Bacillus binary toxins, including Bacillus anthracis toxins consist of two independent proteins, one being the binding component which mediates the internalization into cell of the intracellularly active component. Clostridial binary toxins induce actin cytoskeleton disorganization through mono-ADP-ribosylation of globular actin and are responsible for enteric diseases. Clostridial and Bacillus binary toxins share structurally and functionally related binding components which recognize specific cell receptors, oligomerize, form pores in endocytic vesicle membrane, and mediate the transport of the enzymatic component into the cytosol. Binding components retain the global structure of pore-forming toxins (PFTs) from the cholesterol-dependent cytotoxin family such as perfringolysin. However, their pore-forming activity notably that of clostridial binding components is more related to that of heptameric PFT family including aerolysin and C. perfringens epsilon toxin. This review focuses upon pore-forming activity of clostridial binary toxins compared to other related PFTs. This article is part of a Special Issue entitled: Pore-Forming Toxins edited by Mauro Dalla Serra and Franco Gambale. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Retail wheeling - users, utilities and power producers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kubacki, J. Jr.

    1996-12-31

    Information is outlined on the retail wheeling of electric power. Topics discussed include: SEL mission; average cost per kWh; retail pilot programs; retail wheeling activity; key tasks for industrials; power marketer quote; retail wheeling strategic planning; metered customer load profile; proposed ISO regions; conjunctive billing; interconnection areas; FERC order 888; open access same time information systems; transmission inferconnections; suppliers of energy and capacity; self-generation; FERC Form 714; rebundling unbundled services; key variables: load factor; energy and capacity; metering today; competitive industry configuration; power cost reduction: strategic planning; real-time pricing; prime sources of leverage; likeliness of switching utilities; and Strategic Energymore » Ltd.« less

  3. 78 FR 25431 - Revised Information Collection Activities (FERC-545, FERC-549, and FERC-550); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... registration account with a superior method of authentication. Under the revised procedures, each regulated... pipeline has authorized to submit a particular type of filing. Implementation of these changes will provide...

  4. 75 FR 35003 - Commission Information Collection Activities (FERC Form No. 580); Request; Submitted for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ...'', OMB Control No. 1902-0137. The Public Utility Regulatory Policies Act (PURPA), enacted November 8... adjustment clauses ``to insure efficient use of resources under such clauses.'' \\1\\ In response to the PURPA... review requirement is set forth in two paragraphs of Section 208 of PURPA, 49 Stat. 851; 16 U.S.C. 824d...

  5. 78 FR 55251 - Hydropower Regulatory Efficiency Act of 2013; Notice of Workshop

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-10

    ... Regulatory Efficiency Act of 2013; Notice of Workshop The Federal Energy Regulatory Commission (FERC or... 6 of the Hydropower Regulatory Efficiency Act of 2013. Participants should be prepared to discuss... at https://www.ferc.gov/whats-new/registration/efficiency-act-10-02-13-form.asp by September 25, 2013...

  6. Form-Focused Discovery Activities in English Classes

    ERIC Educational Resources Information Center

    Ogeyik, Muhlise Cosgun

    2011-01-01

    Form-focused discovery activities allow language learners to grasp various aspects of a target language by contributing implicit knowledge by using discovered explicit knowledge. Moreover, such activities can assist learners to perceive and discover the features of their language input. In foreign language teaching environments, they can be used…

  7. 75 FR 44781 - Commission Information Collection Activities (FERC-582); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-29

    ... ``Report/Form/ Annual Charges Report.'' Public utilities and power marketers subject to these annual... Commission issues bills for annual charges, and public utilities and power marketers then must pay the...

  8. Active superconducting devices formed of thin films

    DOEpatents

    Martens, Jon S.; Beyer, James B.; Nordman, James E.; Hohenwarter, Gert K. G.

    1991-05-28

    Active superconducting devices are formed of thin films of superconductor which include a main conduction channel which has an active weak link region. The weak link region is composed of an array of links of thin film superconductor spaced from one another by voids and selected in size and thickness such that magnetic flux can propagate across the weak link region when it is superconducting. Magnetic flux applied to the weak link region will propagate across the array of links causing localized loss of superconductivity in the links and changing the effective resistance across the links. The magnetic flux can be applied from a control line formed of a superconducting film deposited coplanar with the main conduction channel and weak link region on a substrate. The devices can be formed of any type to superconductor but are particularly well suited to the high temperature superconductors since the devices can be entirely formed from coplanar films with no overlying regions. The devices can be utilized for a variety of electrical components, including switching circuits, amplifiers, oscillators and modulators, and are well suited to microwave frequency applications.

  9. 30 CFR 285.618 - What must I do upon completion of approved site assessment activities?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... expiration of your site assessment term, you timely submit a COP meeting the requirements of this subpart, or... your COP for approval or FERC reviews your license application for approval. (b) You are not required... your approved COP or approved FERC license. (c) If, following the technical and environmental review of...

  10. Activated carbon fibers and engineered forms from renewable resources

    DOEpatents

    Baker, Frederick S

    2013-02-19

    A method of producing activated carbon fibers (ACFs) includes the steps of providing a natural carbonaceous precursor fiber material, blending the carbonaceous precursor material with a chemical activation agent to form chemical agent-impregnated precursor fibers, spinning the chemical agent-impregnated precursor material into fibers, and thermally treating the chemical agent-impregnated precursor fibers. The carbonaceous precursor material is both carbonized and activated to form ACFs in a single step. The method produces ACFs exclusive of a step to isolate an intermediate carbon fiber.

  11. Activated carbon fibers and engineered forms from renewable resources

    DOEpatents

    Baker, Frederick S.

    2010-06-01

    A method of producing activated carbon fibers (ACFs) includes the steps of providing a natural carbonaceous precursor fiber material, blending the carbonaceous precursor material with a chemical activation agent to form chemical agent-impregnated precursor fibers, spinning the chemical agent-impregnated precursor material into fibers, and thermally treating the chemical agent-impregnated precursor fibers. The carbonaceous precursor material is both carbonized and activated to form ACFs in a single step. The method produces ACFs exclusive of a step to isolate an intermediate carbon fiber.

  12. Smoking Discriminately Changes the Serum Active and Non-Active Forms of Vitamin B12.

    PubMed

    Shekoohi, Niloofar; Javanbakht, Mohammad Hassan; Sohrabi, Marjan; Zarei, Mahnaz; Mohammadi, Hamed; Djalali, Mahmoud

    2017-06-01

    Smoking may modify the appetite, and consequently affect nutrient intake and serum micronutrients. The effect of smoking on vitamin B12 status has been considered in several studies. The research proposed that organic nitrites, nitro oxide, cyanides, and isocyanides of cigarette smoke interfere with vitamin B12 metabolism, and convert it to inactive forms. This research was carried out to determine the serum level of active and inactive forms of vitamin B12 in male smokers in comparison with male nonsmokers. This is a case-control study, in which the participants were 85 male smokers and 85 male nonsmokers. The serum levels of total and active form of vitamin B12 were measured. Dietary intake was recorded by a quantitative food frequency questionnaire and one-day 24-hour dietary recall method. Independent two sample T test was used to compare quantitative variables between the case and control groups. The serum level of total vitamin B12 was not significantly different between two groups, but serum level of active form of vitamin B12 in the smoking group was significantly lower than non-smoking group (P<0.001). This is one of the first studies that evaluated the serum level of active form of vitamin B12 in smokers in the Iranian community. The results of this study identified that serum level of total vitamin B12 might be not different between smoking and non-smoking people, but the function of this vitamin is disturbed in the body of smokers through the reduction of serum level of active form of vitamin B12.

  13. 76 FR 30738 - Agency Information Collection Activities: Form G-845 and Form G-845 Supplement, Revision of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-26

    ... Collection Activities: Form G-845 and Form G- 845 Supplement, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection under Review: Form G- 845 and Form G-845 Supplement, Document Verification Request and Document Verification Request Supplement; OMB...

  14. 76 FR 46783 - Commission Information Collection Activities (FERC-549); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... 1992. There has been a sharp increase in demand for natural gas outside of the traditional winter... activities and unbundled sales activities of interstate natural gas pipelines and blanket marketing... and to monitor and evaluate transactions and operations of interstate pipelines and blanket marketing...

  15. The International Physical Activity Questionnaire-long form overestimates self-reported physical activity of Brazilian adults.

    PubMed

    Sebastião, E; Gobbi, S; Chodzko-Zajko, W; Schwingel, A; Papini, C B; Nakamura, P M; Netto, A V; Kokubun, E

    2012-11-01

    To explore issues associated with measuring physical activity using the International Physical Activity Questionnaire (IPAQ)-long form in adults living in a mid-sized Brazilian city. A stratified random sampling procedure was used to select a representative sample of adults living in Rio Claro. This yielded 1572 participants who were interviewed using the IPAQ-long form. The data were analysed using standard statistical procedures. Overall, 83% of men and 89% of women reported at least 150 min of combined moderate and/or vigorous physical activity per week. Reliable values of leisure and transportation-related physical activity were observed for both males and females. With regard to the household and work-related physical activity domains, both males and females reported unusually high levels of participation. The IPAQ-long form appears to overestimate levels of physical activity for both males and females, suggesting that the instrument has problems in measuring levels of physical activity in Brazilian adults. Accordingly, caution is warranted before using IPAQ data to support public policy decisions related to physical activity. Copyright © 2012 The Royal Society for Public Health. Published by Elsevier Ltd. All rights reserved.

  16. 76 FR 27077 - Agency Information Collection Activities: Form AR-11 and Form AR-11SR, Extension of an Existing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-10

    ... Collection Activities: Form AR-11 and Form AR- 11SR, Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection under Review: Form AR- 11 and Form AR-11SR, Alien... evaluating whether to revise the Form AR-11 and Form AR-11SR (Forms AR-11). Should USCIS decide to revise...

  17. 77 FR 46086 - Commission Information Collection Activities (FERC-576); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-02

    ... result of a hurricane, earthquake, or other natural disaster, or terrorist activity, (2) serious interruptions to service, and (3) damage to jurisdictional natural gas facilities due to natural disaster or... collection requirements with no changes to the reporting requirements. Abstract: A natural gas company must...

  18. 75 FR 51027 - Commission Information Collection Activities (FERC-917 and FERC-918) 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... public utilities, working through the North American Electric Reliability Corporation (NERC), to develop... required a NERC/NAESB \\3\\ team to draft and review Order No. 890 reliability standards and business... revise reliability standards to require the exchange of data and coordination among transmission...

  19. 75 FR 16492 - Agency Information Collection Activities: Form G-28, and Form G-28I, Revision of an Existing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ... Collection Activities: Form G-28, and Form G- 28I, Revision of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection under Review: Form G- 28, Notice of Entry of Appearance as Attorney or Accredited Representative, and Form G-28I, Notice of Entry of Appearance of Foreign...

  20. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... functions. 366.23 Section 366.23 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE PUBLIC UTILITY HOLDING COMPANY ACT OF 2005, FEDERAL... or narrative description. (b) Transition period. Service companies in holding company systems...

  1. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... functions. 366.23 Section 366.23 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE PUBLIC UTILITY HOLDING COMPANY ACT OF 2005, FEDERAL... or narrative description. (b) Transition period. Service companies in holding company systems...

  2. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... functions. 366.23 Section 366.23 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE PUBLIC UTILITY HOLDING COMPANY ACT OF 2005, FEDERAL... or narrative description. (b) Transition period. Service companies in holding company systems...

  3. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... functions. 366.23 Section 366.23 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE PUBLIC UTILITY HOLDING COMPANY ACT OF 2005, FEDERAL... or narrative description. (b) Transition period. Service companies in holding company systems...

  4. 18 CFR 366.23 - FERC Form No. 60, Annual reports of centralized service companies, and FERC-61, Narrative...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... functions. 366.23 Section 366.23 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE PUBLIC UTILITY HOLDING COMPANY ACT OF 2005, FEDERAL... or narrative description. (b) Transition period. Service companies in holding company systems...

  5. An ELISA method detecting the active form of suPAR.

    PubMed

    Zhou, Xiaolei; Xu, Mingming; Huang, Hailong; Mazar, Andrew; Iqbal, Zafar; Yuan, Cai; Huang, Mingdong

    2016-11-01

    Urokinase plasminogen activator receptor (uPAR) exists in a number of formats in human plasma, including soluble uPAR (suPAR) and uPAR fragments. We developed an ELISA method to detect specifically the active form suPAR, which binds to its natural ligand uPA. The intra CV and inter CV of this ELISA assay is 8.5% and 9.6% respectively, and the assay can recover 99.74% of added recombinant suPAR from 10% plasma. This assay is quite sensitive, capable of detecting down to 15pg/ml of suPAR, and can measure suPAR concentrations in the range of 0.031-8ng/ml with high linear relationship. Plasma samples from pregnant women were also measured for the active form of suPAR with this assay, giving an averaged level of 1.39ng/ml, slightly higher than the level of pooled plasma from healthy donors (0.96ng/ml). This study demonstrates the feasibility to measure the active form of suPAR, which will likely have value in clinical applications. Copyright © 2016. Published by Elsevier B.V.

  6. 75 FR 47804 - Commission Information Collection Activities (FERC-574); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... Commission; and (d) that such State Commission is exercising that jurisdiction. The data required to be filed... in support of its mission. These costs apply to activities which benefit the whole organization...

  7. 75 FR 35001 - Commission Information Collection Activities (FERC-FERC-725E); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... and request for comments. SUMMARY: In compliance with the requirements of section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995, 44 U.S.C. 3506(c)(2)(A) (2006), (Pub. L. No. 104-13), the Federal Energy... electronically must send an original and two (2) paper copies of their comments to: Federal Energy Regulatory...

  8. 78 FR 69843 - Commission Information Collection Activities (Ferc-Ferc-549d); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-21

    ... materials in the Federal Register. Type of Respondents: Intrastate natural gas and Hinshaw pipelines...: Title: Quarterly Transportation and Storage Report for Intrastate Natural Gas and Hinshaw Pipelines. OMB... Report for Intrastate Natural Gas and Hinshaw Pipelines Number of Average burden Estimated Format of...

  9. Hydropower resources at risk: The status of hydropower regulation and development - 1997

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hunt, R.T.; Hunt, J.A.

    This report documents today`s hydropower licensing and development status based on published data as follows: (a) Federal Energy Regulatory Commission (FERC) databases, maintained by FERC`s Office of Hydropower Licensing, of: (1) operating FERC-regulated projects, federal projects, and known unlicensed projects; (2) surrendered licenses; and, (3) recent licensing and relicensing actions; (b) Energy Information Administration (EIA) data on installed capacity and generation from 1949 through 1995 for the various resources used to produce electricity in the U.S.; and, (c) FERC licensing orders, and environmental assessments or environmental impact statements for each individual project relicensed since 1980. The analysis conducted to preparemore » this paper includes the effects of all FERC hydropower licensing actions since 1980, and applies those findings to estimate the costs of hydropower licensing and development activity for the next 15 years. It also quantifies the national cost of hydropower regulation. The future estimates are quite conservative. The are presented in 1996 dollars without speculating on the effects of future inflation, license surrenders, conditions imposed through open-ended license articles, license terms greater than 30 years, or low water years. Instead, they show the most directly predictable influences on licensing outcomes using actual experiences since ECPA (after 1986).« less

  10. 75 FR 35700 - Revisions to Forms, Statements, and Reporting Requirements for Natural Gas Pipelines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... filed in native applications or print-to-PDF format and not in a scanned format. Mail/Hand Delivery... also propose to revise page 520 accordingly. \\1\\ American Gas Association v. FERC, 593 F.3d 14 (D.C....\\14\\ \\14\\ 593 F.3d at 21. 8. Following the court's remand, AGA filed a motion requesting that the...

  11. 76 FR 52253 - Revisions to Forms, Statements, and Reporting Requirements for Natural Gas Pipelines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-22

    ... FERC ] 61,033, at P 1, 7, 37. The Final Rule has a more complete discussion of the procedural history of this case. We will not reiterate that complete history here. 5. In response to the Final Rule... in this fashion, future contracts could be rewritten to achieve this end and we do not wish to open...

  12. 77 FR 28369 - Commission Information Collection Activities (FERC-576); Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-14

    ... the requirements of the Paperwork Reduction Act of 1995, 44 U.S.C. 3506(c)(2)(A), the Federal Energy... hurricane, earthquake, or other natural disaster, or terrorist activity, (2) serious interruptions to... pipeline grid. \\1\\ Public Law 75 688; 15 U.S.C. 717 & 717w. Filings (in accordance with the provisions of...

  13. 18 CFR 35.13 - Filing of changes in rate schedules, tariffs or service agreements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... be reconciled with the utility's most recent FERC Form 1. If the utility has not yet submitted Form 1 for the Test Period, the utility shall submit the relevant Form 1 pages in draft form. (D) The utility.... Balance sheets shall be constructed in accordance with the annual report form for electric utilities...

  14. 18 CFR 35.13 - Filing of changes in rate schedules, tariffs or service agreements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... be reconciled with the utility's most recent FERC Form 1. If the utility has not yet submitted Form 1 for the Test Period, the utility shall submit the relevant Form 1 pages in draft form. (D) The utility.... Balance sheets shall be constructed in accordance with the annual report form for electric utilities...

  15. 18 CFR 35.13 - Filing of changes in rate schedules, tariffs or service agreements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... be reconciled with the utility's most recent FERC Form 1. If the utility has not yet submitted Form 1 for the Test Period, the utility shall submit the relevant Form 1 pages in draft form. (D) The utility.... Balance sheets shall be constructed in accordance with the annual report form for electric utilities...

  16. 18 CFR 35.13 - Filing of changes in rate schedules, tariffs or service agreements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... be reconciled with the utility's most recent FERC Form 1. If the utility has not yet submitted Form 1 for the Test Period, the utility shall submit the relevant Form 1 pages in draft form. (D) The utility.... Balance sheets shall be constructed in accordance with the annual report form for electric utilities...

  17. 18 CFR 35.13 - Filing of changes in rate schedules, tariffs or service agreements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... be reconciled with the utility's most recent FERC Form 1. If the utility has not yet submitted Form 1 for the Test Period, the utility shall submit the relevant Form 1 pages in draft form. (D) The utility.... Balance sheets shall be constructed in accordance with the annual report form for electric utilities...

  18. Differential cellulolytic activity of native-form and C-terminal tagged-form cellulase derived from coptotermes formosanus and expressed in E. coli

    USDA-ARS?s Scientific Manuscript database

    The endogenous cellulase gene (CfEG3a) of Coptotermes formosanus, an economically important pest termite, was cloned and overexpressed in both native form (nCfEG) and C-terminal His-tagged form (tCfEG) in E.coli. Both forms of recombinant cellulases showed hydrolytic activity on cellulosic substrate...

  19. CASPASE-9 CARD:CORE DOMAIN INTERACTIONS REQUIRE A PROPERLY-FORMED ACTIVE SITE

    PubMed Central

    Huber, Kristen L.; Serrano, Banyuhay P.; Hardy, Jeanne A.

    2018-01-01

    Caspase-9 is a critical factor in the initiation of apoptosis, and as a result is tightly regulated by a number of mechanisms. Caspase-9 contains a Caspase Activation and Recruitment Domain (CARD), which enables caspase-9 to form a tight interaction with the apoptosome, a heptameric activating platform. The caspase-9 CARD has been thought to be principally involved in recruitment to the apoptosome, but its roles outside this interaction have yet to be uncovered. In this work we show that the CARD is involved in physical interactions with the catalytic core of caspase-9 in the absence of the apoptosome; this interaction requires a properly formed caspase-9 active site. The active sites of caspases are composed of four extremely mobile loops. When the active-site loops are not properly ordered, the CARD and core domains of caspase-9 do not interact and behave independently, like loosely tethered beads. When the active-site loop bundle is properly ordered, the CARD domain interacts with the catalytic core, forming a single folding unit. Together these findings provide mechanistic insight into a new level of caspase-9 regulation, prompting speculation that the CARD may also play a role in the recruitment or recognition of substrate. PMID:29500231

  20. New Forms of Student Activism: Lobbying, Trusteeing, and Collective Bargaining.

    ERIC Educational Resources Information Center

    Beeler, Kent D.

    1979-01-01

    This article focuses on three new forms of student activism: lobbying, trusteeing, and collective bargaining. Related aspects of student involvement in the political, legal, and consumer areas are discussed briefly. (Author)

  1. NUCLEAR ACTIVITY IS MORE PREVALENT IN STAR-FORMING GALAXIES

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rosario, D. J.; Lutz, D.; Berta, S.

    2013-07-01

    We explore the question of whether low and moderate luminosity active galactic nuclei (AGNs) are preferentially found in galaxies that are undergoing a transition from active star formation (SF) to quiescence. This notion has been suggested by studies of the UV-optical colors of AGN hosts, which find them to be common among galaxies in the so-called Green Valley, a region of galaxy color space believed to be composed mostly of galaxies undergoing SF quenching. Combining the deepest current X-ray and Herschel/PACS far-infrared (FIR) observations of the two Chandra Deep Fields with redshifts, stellar masses, and rest-frame photometry derived from themore » extensive and uniform multi-wavelength data in these fields, we compare the rest-frame U - V color distributions and star formation rate distributions of AGNs and carefully constructed samples of inactive control galaxies. The UV-to-optical colors of AGNs are consistent with equally massive inactive galaxies at redshifts out to z {approx} 2, but we show that such colors are poor tracers of SF. While the FIR distributions of both star-forming AGNs and star-forming inactive galaxies are statistically similar, we show that AGNs are preferentially found in star-forming host galaxies, or, in other words, AGNs are less likely to be found in weakly star-forming or quenched galaxies. We postulate that, among X-ray-selected AGNs of low and moderate accretion luminosities, the supply of cold gas primarily determines the accretion rate distribution of the nuclear black holes.« less

  2. 18 CFR 34.4 - Required exhibits.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Cash Flows and Computation of Interest Coverage on an actual basis and a pro forma basis for the most... Cash Flows must be in the form prescribed for the “Statement of Cash Flows” of the FERC Form No. 1...

  3. 18 CFR 34.4 - Required exhibits.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Cash Flows and Computation of Interest Coverage on an actual basis and a pro forma basis for the most... Cash Flows must be in the form prescribed for the “Statement of Cash Flows” of the FERC Form No. 1...

  4. 18 CFR 34.4 - Required exhibits.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Cash Flows and Computation of Interest Coverage on an actual basis and a pro forma basis for the most... Cash Flows must be in the form prescribed for the “Statement of Cash Flows” of the FERC Form No. 1...

  5. 18 CFR 34.4 - Required exhibits.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Cash Flows and Computation of Interest Coverage on an actual basis and a pro forma basis for the most... Cash Flows must be in the form prescribed for the “Statement of Cash Flows” of the FERC Form No. 1...

  6. 18 CFR 34.4 - Required exhibits.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Cash Flows and Computation of Interest Coverage on an actual basis and a pro forma basis for the most... Cash Flows must be in the form prescribed for the “Statement of Cash Flows” of the FERC Form No. 1...

  7. PROCESS FOR THE PRODUCTION OF AN ACTIVATED FORM OF UO$sub 2$

    DOEpatents

    Polissar, M.J.

    1957-09-24

    A process for producing a highly active form of UO/sub 2/ characterized both by rapid oxidation in air and by rapid chlorination with CCl/sub 4/ vapor at an elevated temperature is reported. In accordance with the process, commercial UO/sub 2/, is subjected to a series of oxidation-reduction operations to produce a form of UC/sub 2/ of enhanced reactivity. By treatimg commercial UO/sub 2/ at a temperature between 335 and 485 deg C with methane, then briefly with an oxygen containing gas and followimg this by a second treatment with a methane containing gas, the original relatively stable charge of UO/sub 2/ will be transformed into an active form of UO/sub 2/.

  8. Process for forming a homogeneous oxide solid phase of catalytically active material

    DOEpatents

    Perry, Dale L.; Russo, Richard E.; Mao, Xianglei

    1995-01-01

    A process is disclosed for forming a homogeneous oxide solid phase reaction product of catalytically active material comprising one or more alkali metals, one or more alkaline earth metals, and one or more Group VIII transition metals. The process comprises reacting together one or more alkali metal oxides and/or salts, one or more alkaline earth metal oxides and/or salts, one or more Group VIII transition metal oxides and/or salts, capable of forming a catalytically active reaction product, in the optional presence of an additional source of oxygen, using a laser beam to ablate from a target such metal compound reactants in the form of a vapor in a deposition chamber, resulting in the deposition, on a heated substrate in the chamber, of the desired oxide phase reaction product. The resulting product may be formed in variable, but reproducible, stoichiometric ratios. The homogeneous oxide solid phase product is useful as a catalyst, and can be produced in many physical forms, including thin films, particulate forms, coatings on catalyst support structures, and coatings on structures used in reaction apparatus in which the reaction product of the invention will serve as a catalyst.

  9. 78 FR 23553 - Trunkline Gas Company, LLC; Notice of Availability of the Environmental Assessment for the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-19

    ... available for public viewing on the FERC's Web site ( www.ferc.gov ) using the eLibrary link. A limited... Affairs, at (866) 208-FERC, or on the FERC Web site ( www.ferc.gov ) using the eLibrary link. Click on the... systems and portions of compressor stations (CS) located in numerous counties in Illinois, Kentucky...

  10. [L forms of Staphylococcus aureus. Behavior of coagulase, hemolytic and desoxyribonuclease activities and antibiotic sensitivity].

    PubMed

    Loschiavo, F; Giarrizzo, S

    1977-01-01

    L Forms derived from strains of coagulase positive Staphylococcus aureus, have, on the whole, preserved their DNAsic, haemolitic and coagulastic activities. L. forms showed high resistence to antibiotics acting on the bacterial cell-wall. The sensibility to other antibiotics was, roughly, analogous for the L forms as well as for the bacterial strains ones, with the exception of the clortetraciclin and the diidrostreptomicin, ehich proved to be comparatively more active on the L forms.

  11. Phosphorylated Nuclear Receptor CAR Forms a Homodimer To Repress Its Constitutive Activity for Ligand Activation

    PubMed Central

    Shizu, Ryota; Osabe, Makoto; Perera, Lalith; Moore, Rick; Sueyoshi, Tatsuya

    2017-01-01

    ABSTRACT The nuclear receptor CAR (NR1I3) regulates hepatic drug and energy metabolism as well as cell fate. Its activation can be a critical factor in drug-induced toxicity and the development of diseases, including diabetes and tumors. CAR inactivates its constitutive activity by phosphorylation at threonine 38. Utilizing receptor for protein kinase 1 (RACK1) as the regulatory subunit, protein phosphatase 2A (PP2A) dephosphorylates threonine 38 to activate CAR. Here we demonstrate that CAR undergoes homodimer-monomer conversion to regulate this dephosphorylation. By coexpression of two differently tagged CAR proteins in Huh-7 cells, mouse primary hepatocytes, and mouse livers, coimmunoprecipitation and two-dimensional gel electrophoresis revealed that CAR can form a homodimer in a configuration in which the PP2A/RACK1 binding site is buried within its dimer interface. Epidermal growth factor (EGF) was found to stimulate CAR homodimerization, thus constraining CAR in its inactive form. The agonistic ligand CITCO binds directly to the CAR homodimer and dissociates phosphorylated CAR into its monomers, exposing the PP2A/RACK1 binding site for dephosphorylation. Phenobarbital, which is not a CAR ligand, binds the EGF receptor, reversing the EGF signal to monomerize CAR for its indirect activation. Thus, the homodimer-monomer conversion is the underlying molecular mechanism that regulates CAR activation, by placing phosphorylated threonine 38 as the common target for both direct and indirect activation of CAR. PMID:28265001

  12. 76 FR 61725 - Agency Information Collection Activities: Case Submission Form, Case Assistance Form; (Form DHS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ..., Case Assistance Form; (Form DHS-7001), Online Ombudsman Form DHS-7001 AGENCY: Office of the Citizenship... System'' to ``Online Ombudsman Form DHS-7001''. The instructions have been updated to reflect the... Services Ombudsman--001 Virtual Ombudsman System (March 2010) to reflect the name change to Online...

  13. 75 FR 70219 - Commission Information Collection Activities (FERC-574); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... exercising that jurisdiction. The data required to be filed by pipeline companies for an exemption are... activities which benefit the whole organization rather than any one particular function or activity. Comments...

  14. Effect of chemical form of selenium on tissue glutathione peroxidase activity in developing rats

    NASA Technical Reports Server (NTRS)

    Lane, Helen W.; Strength, Ralph; Johnson, Janet; White, Marguerite T.

    1991-01-01

    The hypothesis that the stage of development of rats may affect the availability of various forms of selenium for the activity of glutathione peroxidase (GSHPx) in the rat was experimentally investigated. One experiment evaluated the availability of selenium as selenite or selenomethionine for GSPHx activity during three developmental states in rats: fetus and 7-day old and 14-day old nursing pups. In all tissues studied, GSHPx activity was highest in the 14-day-old pups whose dams were in the selenomethionine group. Rat pups given intraperitoneal selenite had higher liver and kidney GSHPx activity than pups given the same amount of selenium as intraperitoneal selenomethionine. In a second experiment, all dams were fed the same basal diet and pups were weaned to diets containing one of two levels of selenium and one of three forms of selenium (selenite, selenomethionine, or selenocystine). The results also supported the hypothesis these dietary forms of selenium are differentially available for GSHPx activity.

  15. 78 FR 35639 - Agency Information Collection Activities: Application To Extend/Change Nonimmigrant Status, Form...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-13

    ... DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services [OMB Control Number 1615-0003] Agency Information Collection Activities: Application To Extend/ Change Nonimmigrant Status, Form...) Title of the Form/Collection: Application to Extend/Change Nonimmigrant Status. (3) Agency form number...

  16. 76 FR 64379 - Agency Information Collection Activities: Proposed Collection; Comments Requested; eForm 6 Access...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ...] Agency Information Collection Activities: Proposed Collection; Comments Requested; eForm 6 Access Request... Form/Collection: eForm 6 Access Request. (3) Agency form number, if any, and the applicable component...: Respondents must complete the eForm 6 Access Request form in order to receive a user ID and password to obtain...

  17. Piezo proteins are pore-forming subunits of mechanically activated channels.

    PubMed

    Coste, Bertrand; Xiao, Bailong; Santos, Jose S; Syeda, Ruhma; Grandl, Jörg; Spencer, Kathryn S; Kim, Sung Eun; Schmidt, Manuela; Mathur, Jayanti; Dubin, Adrienne E; Montal, Mauricio; Patapoutian, Ardem

    2012-02-19

    Mechanotransduction has an important role in physiology. Biological processes including sensing touch and sound waves require as-yet-unidentified cation channels that detect pressure. Mouse Piezo1 (MmPiezo1) and MmPiezo2 (also called Fam38a and Fam38b, respectively) induce mechanically activated cationic currents in cells; however, it is unknown whether Piezo proteins are pore-forming ion channels or modulate ion channels. Here we show that Drosophila melanogaster Piezo (DmPiezo, also called CG8486) also induces mechanically activated currents in cells, but through channels with remarkably distinct pore properties including sensitivity to the pore blocker ruthenium red and single channel conductances. MmPiezo1 assembles as a ∼1.2-million-dalton homo-oligomer, with no evidence of other proteins in this complex. Purified MmPiezo1 reconstituted into asymmetric lipid bilayers and liposomes forms ruthenium-red-sensitive ion channels. These data demonstrate that Piezo proteins are an evolutionarily conserved ion channel family involved in mechanotransduction.

  18. 76 FR 66920 - Commission Information Collection Activities (FERC-549); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-28

    ... sharp increase in demand for natural gas outside of the traditional winter months. Withdrawals and... activities and unbundled sales activities of interstate natural gas pipelines and blanket marketing... and to monitor and evaluate transactions and operations of interstate pipelines and blanket marketing...

  19. A pore-forming protein implements VLR-activated complement cytotoxicity in lamprey.

    PubMed

    Wu, Fenfang; Feng, Bo; Ren, Yong; Wu, Di; Chen, Yue; Huang, Shengfeng; Chen, Shangwu; Xu, Anlong

    2017-01-01

    Lamprey is a basal vertebrate with a unique adaptive immune system, which uses variable lymphocyte receptors (VLRs) for antigen recognition. Our previous study has shown that lamprey possessed a distinctive complement pathway activated by VLR. In this study, we identified a natterin family member-lamprey pore-forming protein (LPFP) with a jacalin-like lectin domain and an aerolysin-like pore-forming domain. LPFP had a high affinity with mannan and could form oligomer in the presence of mannan. LPFP could deposit on the surface of target cells, form pore-like complex resembling a wheel with hub and spokes, and mediate powerful cytotoxicity on target cells. These pore-forming proteins along with VLRs and complement molecules were essential for the specific cytotoxicity against exogenous pathogens and tumor cells. This unique cytotoxicity implemented by LPFP might emerge before or in parallel with the IgG-based classical complement lytic pathway completed by polyC9.

  20. Education Technologies in Addressing the Problem of Forming the Socially Active Individual

    ERIC Educational Resources Information Center

    Popova, Irina N.

    2016-01-01

    The article is devoted to the analysis of technological support of the educational process in solving the problem of forming the socially active individual. The authors studied the value of the category "social activity" and analyzed educational technologies that have an impact on its formation. The obtained results gave the possibility…

  1. 76 FR 74781 - Sawgrass Storage LLC; Notice of Availability of the Environmental Assessment for the Proposed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-01

    ... Affairs, at (866) 208-FERC, or on the FERC Web site ( http://www.ferc.gov ) using the eLibrary link. Click... Midcontinent Express Pipeline's interstate pipeline system; and appurtenant facilities. The FERC staff mailed... interested individuals and groups; newspapers and libraries in the project area; and parties to this...

  2. 75 FR 51094 - Agency Information Collection Activities: Form N-600; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ...-0057] Agency Information Collection Activities: Form N-600; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection under Review; Form N- 600... be evaluating whether to revise the Form N-600. Should USCIS decide to revise Form N-600 we will...

  3. 76 FR 24908 - Agency Information Collection Activities: Form G-639; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-03

    ... Collection Activities: Form G-639; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form G- 639, Freedom of Information/Privacy Act... Form G-639. Should USCIS decide to revise Form G-639 we will advise the [[Page 24909

  4. 78 FR 4858 - Agency Information Collection Activities: Immigrant Petition for Alien Workers, Form I-140...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-23

    ...-0015] Agency Information Collection Activities: Immigrant Petition for Alien Workers, Form I-140... Approved Collection. (2) Title of the Form/Collection: Immigrant Petition for Alien Worker. (3) Agency form... other for-profit. The information furnished on Form I-140 will be used by USCIS to classify aliens under...

  5. 77 FR 65706 - Agency Information Collection Activities: Immigrant Petition for Alien Worker, Form I-140...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ...-0015] Agency Information Collection Activities: Immigrant Petition for Alien Worker, Form I-140... Form/Collection: Immigrant Petition for Alien Worker. (3) Agency form number, if any, and the... information furnished on Form I-140 will be used by USCIS to classify aliens under sections 203(b)(1), 203(b...

  6. 75 FR 51096 - Agency Information Collection Activities: Form N-400; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... Collection Activities: Form N-400; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form N- 400, Application for Naturalization... the Form N-400. Should USCIS decide to revise Form N-400 we will advise the public when we publish the...

  7. 75 FR 51096 - Agency Information Collection Activities: Form N-470; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... Collection Activities: Form N-470; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form N- 470, Application To Preserve Residence... evaluating whether to revise the Form N-470. Should USCIS decide to revise Form N-470 we will advise the...

  8. 77 FR 47049 - Commission Information Collection Activities; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-07

    ... collection FERC-914 (Cogeneration and Small Power Production--Tariff Filings) to the Office of Management and... statement]; (2) ensuring that these facilities are using their thermal output in a productive and beneficial... Annual Burden\\3\\: The Commission estimates the total Public Reporting Burden for this information...

  9. Comparative analysis of activator-Eσ54 complexes formed with nucleotide-metal fluoride analogues

    PubMed Central

    Burrows, Patricia C.; Joly, Nicolas; Nixon, B. Tracy; Buck, Martin

    2009-01-01

    Bacterial RNA polymerase (RNAP) containing the major variant σ54 factor forms open promoter complexes in a reaction in which specialized activator proteins hydrolyse ATP. Here we probe binding interactions between σ54-RNAP (Eσ54) and the ATPases associated with various cellular activities (AAA+) domain of the Escherichia coli activator protein, PspF, using nucleotide-metal fluoride (BeF and AlF) analogues representing ground and transition states of ATP, which allow complexes (that are otherwise too transient with ATP) to be captured. We show that the organization and functionality of the ADP–BeF- and ADP–AlF-dependent complexes greatly overlap. Our data support an activation pathway in which the initial ATP-dependent binding of the activator to the Eσ54 closed complex results in the re-organization of Eσ54 with respect to the transcription start-site. However, the nucleotide-dependent binding interactions between the activator and the Eσ54 closed complex are in themselves insufficient for forming open promoter complexes when linear double-stranded DNA is present in the initial closed complex. PMID:19553192

  10. 76 FR 27078 - Agency Information Collection Activities: Form N-426, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-10

    ... Collection Activities: Form N-426, Extension of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form N- 426, Request for Certification of... whether to revise the Form N-426. Should USCIS decide to revise Form N-426 we will advise the public when...

  11. 77 FR 76015 - Prior Notice of Activity Under Blanket Certificate; Dominion Transmission, Inc.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-26

    ... Dominion's authorization in Docket No. CP88-712-000, 52 FERC ]61,112 (1990) for authority to replace TL-465... pipeline to meet Class 3 design requirements, as more fully detailed in the Application. Questions...

  12. 77 FR 65702 - Agency Information Collection Activities: Refugee/Asylee Relative Petition, Form Number I-730...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ...-0037] Agency Information Collection Activities: Refugee/Asylee Relative Petition, Form Number I-730... request. (2) Title of the Form/Collection: Refugee/Asylee Relative Petition. (3) Agency form number, if... households. Form I- 730 will be used by an asylee or refugee to file on behalf of his or her spouse and/or...

  13. 77 FR 11519 - Commission Information Collection Activities; Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-27

    ... collection, FERC-585, Reporting of Electric Energy shortages and Contingency Plans under PURPA. DATES... shortages and Contingency Plans under PURPA. OMB Control No.: 1902-0138. Type of Request: Three-year... Energy Shortages and Contingency Plans under PURPA'', is used by the Commission to implement the...

  14. The development and characterization of an ELISA specifically detecting the active form of cathepsin K.

    PubMed

    Sun, S; Karsdal, M A; Bay-Jensen, A C; Sørensen, M G; Zheng, Q; Dziegiel, M H; Maksymowych, W P; Henriksen, K

    2013-10-01

    Cathepsin K plays essential roles in bone resorption and is intensely investigated as a therapeutic target for the treatment of osteoporosis. Hence an assessment of the active form of cathepsin K may provide important biological information in metabolic bone diseases, such as osteoporosis or ankylosing spondylitis. Presently there are no robust assays for the assessment of active cathepsin K in serum, and therefore an ELISA specifically detecting the N-terminal of the active form of cathepsin K was developed. The assay was technically robust, with a lowest limit of detection (LOD) of 0.085 ng/mL. The average intra- and inter-assay CV% were 6.60% and 8.56% respectively. The dilution recovery and spike recovery tests in human serum were within 100±20% within the range of the assay. A comparison of latent and active cathepsin K confirmed specificity towards the active form. Quantification of the levels of active cathepsin K in supernatants of purified human osteoclasts compared to corresponding macrophages showed a 30-fold induction (p<0.001). In contrast, in serum samples from osteoporotic women on estrogen or bisphosphonate therapy and from ankylosing spondylitis patients no clinically relevant differences were observed. In summary, we have developed a robust and sensitive assay specifically detecting the active form of cathepsin K; however, while it monitors osteoclasts with high specificity in vitro, it appears that circulating levels of active cathepsin K do not reflect bone changes under these circumstances. Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  15. 75 FR 11192 - Agency Information Collection Activities: Form I-730, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-10

    ...-0037] Agency Information Collection Activities: Form I-730, Revision of an Existing Information Collection Request; Comment Request ACTION: 60-Day Notice of Information Collection under Review: Form I- 730..., mechanical, or other technological collection techniques, or other forms of information technology, e.g...

  16. 75 FR 43535 - Agency Information Collection Activities: Form N-644, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-26

    ... Collection Activities: Form N-644, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day notice of information collection under review: Form N- 644, Application for Posthumous...-day comment period USCIS would be evaluating whether to revise the Form [[Page 43536

  17. 75 FR 47822 - Agency Information Collection Activities: Form G-639, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... Collection Activities: Form G-639, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form G- 639, Freedom of Information/Privacy... technological collection techniques or other forms of information technology, e.g., permitting electronic...

  18. 77 FR 33760 - Agency Information Collection Activities: Form G-646, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... Collection Activities: Form G-646, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form G- 646, Sworn Statement of Refugee... technological collection techniques or other forms of information technology, e.g., permitting electronic...

  19. 76 FR 11807 - Agency Information Collection Activities: Form G-646, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... Collection Activities: Form G-646, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form G- 646, Sworn Statement of Refugee... technological collection techniques or other forms of information technology, e.g., permitting electronic...

  20. 76 FR 81928 - Kansas City, KS, Board of Public Utilities; Notice of Petition for Waiver

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    .... \\1\\ Open Access Same-Time Information System and Standards of Conduct, Order No. 889, FERC Stats. & Regs. ] 31,035 (1996), order on reh'g, Order No. 889-A, FERC Stats. & Regs. ] 31,049 (1997), reh'g... Providers, Order No. 2004, FERC Stats. & Regs. ] 31,155 (2003), order on reh'g, Order No. 2004-A, FERC Stats...

  1. 78 FR 25434 - Minnesota Municipal Power Agency; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ..., 61 FR 21,737, FERC Stats. & Regs. ] 31,035 (1996), order on reh'g, Order No. 889-A, 62 FR 12,484, FERC Stats. & Regs. ] 31,049 (1997) (Order No. 889-A), reh'g denied, Order No. 889-B, 81 FERC ] 61,253.... Cir. 2000). \\2\\ Standards of Conduct for Transmission Providers, Order No. 2004, FERC Stats. & Regs...

  2. 78 FR 46177 - Third-Party Provision of Ancillary Services; Accounting and Financial Reporting for New Electric...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ...The Federal Energy Regulatory Commission (Commission) is revising its regulations to foster competition and transparency in ancillary services markets. The Commission is revising certain aspects of its current market-based rate regulations, ancillary services requirements under the pro forma open-access transmission tariff (OATT), and accounting and reporting requirements. Specifically, the Commission is revising its regulations to reflect reforms to its Avista policy governing the sale of ancillary services at market-based rates to public utility transmission providers. The Commission is also requiring each public utility transmission provider to add to its OATT Schedule 3 a statement that it will take into account the speed and accuracy of regulation resources in its determination of reserve requirements for Regulation and Frequency Response service, including as it reviews whether a self-supplying customer has made ``alternative comparable arrangements'' as required by the Schedule. The final rule also requires each public utility transmission provider to post certain Area Control Error data as described in the final rule. Finally, the Commission is revising the accounting and reporting requirements under its Uniform System of Accounts for public utilities and licensees and its forms, statements, and reports, contained in FERC Form No. 1, Annual Report of Major Electric Utilities, Licensees and Others, FERC Form No. 1-F, Annual Report for Nonmajor Public Utilities and Licensees, and FERC Form No. 3-Q, Quarterly Financial Report of Electric Utilities, Licensees, and Natural Gas Companies, to better account for and report transactions associated with the use of energy storage devices in public utility operations.

  3. 77 FR 3758 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ...: Attachment H Schedule 7 Compliance Filing to be effective 6/1/2011. Filed Date: 1/13/12. Accession Number... filings: Docket Numbers: QF12-159-000. Applicants: City of Kinston, NC. Description: FERC Form 556 of City...

  4. 77 FR 65898 - Agency Information Collection Activities: InfoPass System, No Form Number; Extension, Without...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-31

    ...-0113] Agency Information Collection Activities: InfoPass System, No Form Number; Extension, Without...) Title of the Form/Collection: InfoPass System. (3) Agency form number, if any, and the applicable... InfoPass system allows an applicant or petitioner to schedule an interview appointment with USCIS...

  5. 75 FR 70278 - Agency Information Collection Activities: Form N-600, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... Collection Activities: Form N-600, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 600, Application for Certificate of... Homeland Security sponsoring the collection: Form N-600; U.S. Citizenship and Immigration Services (USCIS...

  6. 76 FR 45844 - Agency Information Collection Activities: Form N-426, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... Collection Activities: Form N-426, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 426, Request for Certification of... Department of Homeland Security sponsoring the collection: Form N-426. U.S. Citizenship and Immigration...

  7. 76 FR 39415 - Agency Information Collection Activities: Form N-644, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... Collection Activities: Form N-644, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 644, Application for Posthumous... Homeland Security sponsoring the collection: Form N-644; U.S. Citizenship and Immigration Services (USCIS...

  8. 76 FR 59710 - Agency Information Collection Activities: Form N-600; Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-27

    ... Collection Activities: Form N-600; Revision of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form N- 600, Application for Certificate of... the applicable component of the Department of Homeland Security sponsoring the collection: Form N-600...

  9. 75 FR 30050 - Agency Information Collection Activities: Form N-648, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-28

    ... Collection Activities: Form N-648, Revision of an Existing Information Collection Request; Comment Request ACTION: 30[dash]Day Notice of Information Collection Under Review: Form N-648, Medical Certification for..., and the applicable component of the Department of Homeland Security sponsoring the collection: Form N...

  10. 75 FR 5099 - Agency Information Collection Activities: Form N-648, Revision of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Collection Activities: Form N-648, Revision of an Existing Information Collection Request; Comment Request ACTION: 60-day notice of information collection under review: Form N- 648, Medical Certification for..., and the applicable component of the Department of Homeland Security sponsoring the collection: Form N...

  11. 77 FR 128 - Agency Information Collection Activities: Form N-600, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... Collection Activities: Form N-600, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 600, Application for Certificate of..., and the applicable component of the Department of Homeland Security sponsoring the collection: Form N...

  12. 76 FR 63320 - Agency Information Collection Activities: Form N-470, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... Collection Activities: Form N-470, Revision of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form N- 470, Application to Preserve... public comment period. Subsequently, USCIS decided to conduct a comprehensive revision of Form N-470...

  13. 75 FR 70277 - Agency Information Collection Activities: Form N-400, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... Collection Activities: Form N-400, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 400, Application for Naturalization... Security sponsoring the collection: Form N-400; U.S. Citizenship and Immigration Services (USCIS). (4...

  14. 76 FR 69275 - Agency Information Collection Activities: Form N-400, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-08

    ... Collection Activities: Form N-400, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 400, Application for Naturalization... sponsoring the collection: Form N-400. U.S. Citizenship and Immigration Services. (4) Affected public who...

  15. 76 FR 78674 - Agency Information Collection Activities: Form N-470, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-19

    ... Collection Activities: Form N-470, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 470, Application To Preserve... of Homeland Security sponsoring the collection: Form N-470. U.S. Citizenship and Immigration Services...

  16. 76 FR 63322 - Agency Information Collection Activities: Form G-28, Revision of an Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... Collection Activities: Form G-28, Revision of an Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form G- 28, Notice of Entry of Appearance as..., mechanical, or other technological collection techniques, or other forms of information technology, e.g...

  17. 76 FR 53929 - Agency Information Collection Activities: Form G-639, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ... Collection Activities: Form G-639, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form G- 639, Freedom of Information/Privacy... other forms of information technology, e.g., permitting electronic submission of responses. Overview of...

  18. Structure of the active form of human origin recognition complex and its ATPase motor module

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tocilj, Ante; On, Kin Fan; Yuan, Zuanning

    Binding of the Origin Recognition Complex (ORC) to origins of replication marks the first step in the initiation of replication of the genome in all eukaryotic cells. Here, we report the structure of the active form of human ORC determined by X-ray crystallography and cryo-electron microscopy. The complex is composed of an ORC1/4/5 motor module lobe in an organization reminiscent of the DNA polymerase clamp loader complexes. A second lobe contains the ORC2/3 subunits. The complex is organized as a double-layered shallow corkscrew, with the AAA+ and AAA+-like domains forming one layer, and the winged-helix domains (WHDs) forming a topmore » layer. CDC6 fits easily between ORC1 and ORC2, completing the ring and the DNA-binding channel, forming an additional ATP hydrolysis site. Analysis of the ATPase activity of the complex provides a basis for understanding ORC activity as well as molecular defects observed in Meier-Gorlin Syndrome mutations.« less

  19. 77 FR 65706 - Agency Information Collection Activities: Request for the Return of Original Documents, Form...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ...-0100] Agency Information Collection Activities: Request for the Return of Original Documents, Form... Collection. (2) Title of the Form/Collection: Request for the Return of Original Documents. (3) Agency form... obtain original document(s) contained in an alien file. (5) An estimate of the total number of...

  20. 77 FR 47426 - Agency Information Collection Activities: Request for the Return of Original Documents, Form...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ...-0100] Agency Information Collection Activities: Request for the Return of Original Documents, Form.... (2) Title of the Form/Collection: Request for the Return of Original Documents. (3) Agency form... obtain original document(s) contained in an alien file. (5) An estimate of the total number of...

  1. 77 FR 71432 - Agency Information Collection Activities: Immigrant Petition by Alien Entrepreneur, Form I-526...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-30

    ...-0026] Agency Information Collection Activities: Immigrant Petition by Alien Entrepreneur, Form I-526.../Collection: Immigrant Petition by Alien Entrepreneur. (3) Agency form number, if any, and the applicable... abstract: Primary: Individuals or Households. This form is used by the USCIS to determine if an alien can...

  2. 75 FR 71451 - Agency Information Collection Activities: Form N-470, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-23

    ... Collection Activities: Form N-470, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 470, Application to Preserve... collection: Form N-470; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be...

  3. 75 FR 5098 - Agency Information Collection Activities: Form N-565, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Collection Activities: Form N-565, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-day notice of information collection under review: Form N- 565, Application for Replacement... collection: Form N-565; U.S. Citizenship and Immigration Services (USCIS). (4) Affected public who will be...

  4. 76 FR 53144 - Agency Information Collection Activities: Form N-336; Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-25

    ... Collection Activities: Form N-336; Revision of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form N- 336, Request for Hearing on a... Homeland Security sponsoring the collection: Form N-336; U.S. Citizenship and Immigration Services (USCIS...

  5. 75 FR 70277 - Agency Information Collection Activities: Form N-336, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... Collection Activities: Form N-336, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 336, Request for Hearing on a... Security sponsoring the collection: Form N-336; U.S. Citizenship and Immigration Services (USCIS). (4...

  6. 75 FR 51095 - Agency Information Collection Activities: Form N-336; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ...-0050] Agency Information Collection Activities: Form N-336; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection under Review; Form N- 336..., 2010. During this 60 day period, USCIS will be evaluating whether to revise the Form N-336. Should...

  7. 75 FR 21013 - Agency Information Collection Activities: Form N-644; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-22

    ... Collection Activities: Form N-644; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form N- 644, Application for Posthumous... until June 21, 2010. During this 60 day period, USCIS will be evaluating whether to revise the Form N...

  8. 78 FR 44424 - Revisions to Page 700 of FERC Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-24

    ... original cost (TOC) rate base information provided on line 5 of page 700 by requiring the reporting of the three subparts of the TOC rate base: (1) Rate Base--Original Cost (proposed line 5a); (2) Rate Base... oil pipeline's TOC Rate Base. Consequently, the NOPR proposed to move the TOC rate base from line 5 to...

  9. 77 FR 59348 - Revisions to Page 700 of FERC Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-27

    .... The components of an oil pipeline's rate base are governed by the Trended Original Cost Methodology... ratemaking methodology to the Trended Original Cost methodology as adopted in Opinion 154-B. The SRB was to... trended original cost methodology divides the nominal return on equity component of the cost of service...

  10. 75 FR 41216 - Agency Information Collection Activities: Form N-644, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... Collection Activities: Form N-644, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 644, Application for Posthumous...

  11. 76 FR 43336 - Agency Information Collection Activities: Form AR-11, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... Collection Activities: Form AR-11, Extension of an Existing Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection under Review: Form AR- 11, Alien's Change of Address Card... collection: Form AR-11. U.S. Citizenship and Immigration Services. (4) Affected public who will be asked or...

  12. 76 FR 21913 - Agency Information Collection Activities: Form N-644; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-19

    ... Collection Activities: Form N-644; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form N- 644, Application for Posthumous... 60 days until June 20, 2011 this 60-day period, USCIS will be evaluating whether to revise the Form N...

  13. 75 FR 78264 - Agency Information Collection Activities: Form N-336, Revision to an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-15

    ...-0050] Agency Information Collection Activities: Form N-336, Revision to an Existing Information Collection; Comment Request ACTION: 30-Day notice of information collection under review: Form N- 336... announcing the extension of the Form N-336. The 60-day notice announced that during the 60-day comment period...

  14. 76 FR 4150 - Reports, Forms and Recordkeeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-24

    ... automated collection techniques or other forms of information technology. A Comment to OMB is most effective.... NHTSA-2010-0023] Reports, Forms and Recordkeeping Requirements; Agency Information Collection Activity... announces that the Information Collection Request (ICR) abstracted below has been forwarded to the Office of...

  15. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Parsons, B.; Porter, K.

    In 1996, the Federal Energy Regulatory Commission (FERC) issued Order 888, which required transmission-owning utilities under FERC jurisdiction to provide open access transmission service to eligible wholesale power customers. Among other things, the elements of electric service are unbundled, meaning that wind project developers must not only find a taker for the energy but also potentially make interconnection, ancillary service, and transmission arrangements for their wind projects. In 1999, the FERC issued Order 2000, which required transmission-owning utilities to file an intent with FERC on whether they have joined or plan to join a regional transmission organization (RTO). Order 2000more » also required RTOs to meet certain criteria and be approved by FERC in order to begin operations as an RTO. More recently, FERC said it would issue a Notice of Proposed Rulemaking in 2002 on certain requirements and services, often termed''standard market design'' (SMD), that must be included in all transmission tariffs filed at FERC. This paper discusses the chronology of open access transmission issues, from Order 888 onward, and reviews some of the important issues raised by FERC's SMD initiative.« less

  16. 76 FR 69276 - Agency Information Collection Activities: Form N-336, Revision of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-08

    ... Activities: Form N-336, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form N- 336, Request for Hearing on a Decision in... collection: Form N-336. U.S. Citizenship and Immigration Services. (4) Affected public who will be asked or...

  17. 75 FR 23785 - Agency Information Collection Activities: Form G-639; Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-04

    ... Collection Activities: Form G-639; Extension of an Existing Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review; Form G- 639, Freedom of Information/Privacy Act... until July 6, 2010. During this 60 day period, USCIS will be evaluating whether to revise the Form G-639...

  18. 77 FR 70996 - Commission Information Collection Activities; Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-28

    ... Companies and Service Companies Subject to PUHCA). OMB Control No.: 1902-0215. Type of Request: Three-year... company's functions during the prior calendar year (FERC-61). In complying, a holding company may make a... level of transparency that: (1) Helps protect ratepayers from pass-through of improper service company...

  19. 78 FR 24429 - Agency Information Collection Activities: Inter-Agency Alien Witness and Informant Record, Form I...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-25

    ...-0046] Agency Information Collection Activities: Inter-Agency Alien Witness and Informant Record, Form I... the Form/Collection: Inter-Agency Alien Witness and Informant Record. (3) Agency form number, if any... Government. Form I-854 is used by law enforcement agencies to bring alien witnesses and informants to the...

  20. 75 FR 61508 - Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... Activities: Canadian Border Boat Landing Permit (CBP Form I-68) AGENCY: U.S. Customs and Border Protection... information collection requirement concerning the Canadian Border Boat Landing Permit (Form I- [[Page 61509... Boat Landing Permit. OMB Number: 1651-0108. Form Number: CBP Form I-68. Abstract: The Canadian Border...

  1. 76 FR 66707 - Commission Information Collection Activities (FERC-1 and FERC-1F); Comment Request; Submitted for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-27

    ... provided by the Commission's chart of accounts and related accounting instructions permits comparability... accounting records using dissimilar accounting methods and classifications to record similar transactions and... information. The estimate of cost for respondents is based upon salaries for professional and clerical support...

  2. Structure of the active form of human origin recognition complex and its ATPase motor module

    PubMed Central

    Tocilj, Ante; On, Kin Fan; Yuan, Zuanning; Sun, Jingchuan; Elkayam, Elad; Li, Huilin; Stillman, Bruce; Joshua-Tor, Leemor

    2017-01-01

    Binding of the Origin Recognition Complex (ORC) to origins of replication marks the first step in the initiation of replication of the genome in all eukaryotic cells. Here, we report the structure of the active form of human ORC determined by X-ray crystallography and cryo-electron microscopy. The complex is composed of an ORC1/4/5 motor module lobe in an organization reminiscent of the DNA polymerase clamp loader complexes. A second lobe contains the ORC2/3 subunits. The complex is organized as a double-layered shallow corkscrew, with the AAA+ and AAA+-like domains forming one layer, and the winged-helix domains (WHDs) forming a top layer. CDC6 fits easily between ORC1 and ORC2, completing the ring and the DNA-binding channel, forming an additional ATP hydrolysis site. Analysis of the ATPase activity of the complex provides a basis for understanding ORC activity as well as molecular defects observed in Meier-Gorlin Syndrome mutations. DOI: http://dx.doi.org/10.7554/eLife.20818.001 PMID:28112645

  3. Reversible Activation of Halophilic β-lactamase from Methanol-Induced Inactive Form: Contrast to Irreversible Inactivation of Non-Halophilic Counterpart.

    PubMed

    Tokunaga, Hiroko; Maeda, Junpei; Arakawa, Tsutomu; Tokunaga, Masao

    2017-06-01

    Effects of a water-miscible organic solvent, methanol, on the structure and activity of halophilic β-lactamase derived from Chromohalobacter sp.560 (HaBla), were investigated by means of circular dichroism (CD) measurement and enzymatic activity determination. Beta-lactamase activity was enhanced about 1.2-fold in the presence of 10-20% methanol. CD measurement of HaBla revealed different structures depending on the methanol concentration: native-like active form (Form I) in 10-20% methanol and methanol-induced inactive form at higher concentration (Form II in 40-60% and Form III in 75-80% methanol). Incubation of HaBla with 40% methanol led to the complete loss of activity within ~80 min accompanied by the formation of Form II, whose activity was recovered promptly up to ~80% of full activity upon dilution of the methanol concentration to 10%. In addition, when the protein concentration was sufficiently high (e.g., 0.7 mg/ml), HaBla activity of Form III in 75% methanol could be recovered in the same way (with slightly slower recovery rate), upon dilution of the methanol concentration. In contrast, non-halophilic β-lactamase from Escherichia coli K12 strain MG1655 (EcBla) was irreversibly denatured in the presence of 40% methanol. HaBla showed remarkable ability to renature from the methanol-induced inactive states.

  4. Large-scale patterns formed by solar active regions during the ascending phase of cycle 21

    NASA Astrophysics Data System (ADS)

    Gaizauskas, V.; Harvey, K. L.; Harvey, J. W.; Zwaan, C.

    1983-02-01

    Synoptic maps of photospheric magnetic fields prepared at the Kitt Peak National Observatory are used in investigating large-scale patterns in the spatial and temporal distribution of solar active regions for 27 solar rotations between 1977 and 1979. The active regions are found to be distributed in 'complexes of activity' (Bumba and Howard, 1965). With the working definition of a complex of activity based on continuity and proximity of the constituent active regions, the phenomenology of complexes is explored. It is found that complexes of activity form within one month and that they are typically maintained for 3 to 6 solar rotations by fresh injections of magnetic flux. During the active lifetime of a complex of activity, the total magnetic flux in the complex remains steady to within a factor of 2. The magnetic polarities are closely balanced, and each complex rotates about the sun at its own special, constant rate. In certain cases, the complexes form two diverging branches.

  5. The Envelope Glycoprotein of Friend Spleen Focus-Forming Virus Covalently Interacts with and Constitutively Activates a Truncated Form of the Receptor Tyrosine Kinase Stk

    PubMed Central

    Nishigaki, Kazuo; Thompson, Delores; Hanson, Charlotte; Yugawa, Takashi; Ruscetti, Sandra

    2001-01-01

    The Friend spleen focus-forming virus (SFFV) encodes a unique envelope glycoprotein, gp55, which allows erythroid cells to proliferate and differentiate in the absence of erythropoietin (Epo). SFFV gp55 has been shown to interact with the Epo receptor complex, causing constitutive activation of various signal-transducing molecules. When injected into adult mice, SFFV induces a rapid erythroleukemia, with susceptibility being determined by the host gene Fv-2, which was recently shown to be identical to the gene encoding the receptor tyrosine kinase Stk/Ron. Susceptible, but not resistant, mice encode not only full-length Stk but also a truncated form of the kinase, sf-Stk, which may mediate the biological effects of SFFV infection. To determine whether expression of SFFV gp55 leads to the activation of sf-Stk, we expressed sf-Stk, with or without SFFV gp55, in hematopoietic cells expressing the Epo receptor. Our data indicate that sf-Stk interacts with SFFV gp55 as well as gp55P, the biologically active form of the viral glycoprotein, forming disulfide-linked complexes. This covalent interaction, as well as noncovalent interactions with SFFV gp55, results in constitutive tyrosine phosphorylation of sf-Stk and its association with multiple tyrosine-phosphorylated signal-transducing molecules. In contrast, neither Epo stimulation in the absence of SFFV gp55 expression nor expression of a mutant of SFFV that cannot interact with sf-Stk was able to induce tyrosine phosphorylation of sf-Stk or its association with any signal-transducing molecules. Covalent interaction of sf-Stk with SFFV gp55 and constitutive tyrosine phosphorylation of sf-Stk can also be detected in an erythroleukemia cell line derived from an SFFV-infected mouse. Our results suggest that SFFV gp55 may mediate its biological effects in vivo by interacting with and activating a truncated form of the receptor tyrosine kinase Stk. PMID:11483734

  6. 75 FR 59707 - Electric Quarterly Reports; BM2 LLC; DJGW, LLC; Order on Intent To Revoke Market-Based Rate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-28

    .... 2001, FERC Stats. & Regs. ] 31,127, reh'g denied, Order No. 2001-A, 100 FERC ] 61,074, reconsideration...-based rates.\\[2]\\ \\2\\ Order No. 2001, FERC Stats & Regs. ] 31,127 at P 222. 4. The Commission further...

  7. 77 FR 5508 - Order on Intent To Revoke Market-Based Rate Authority

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    .... 2001, FERC Stats. & Regs. ] 31,127, reh'g denied, Order No. 2001-A, 100 FERC ] 61,074, reconsideration... market-based rates.\\2\\ \\2\\ Order No. 2001, FERC Stats & Regs. ] 31,127 at P 222. 4. The Commission...

  8. 75 FR 40817 - City of Pella, Iowa, v. Midwest Independent Transmission System Operator, Inc., MidAmerican...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-14

    ... Public Utilities and Transmitting Utilities, Order No. 888, FERC Stats. & Regs. ] 31,036 (1996), order on reh'g, Order No. 888-A, FERC Stats. & Regs. ] 31,048, order on reh'g, Order No. 888-B, 81 FERC ] 61...

  9. 77 FR 59607 - El Paso Natural Gas Company; Notice of Public Scoping Meetings for the Planned SASABE Lateral...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ... Schoolhouse Drive, Sasabe, AZ 85633. \\1\\ A Spanish-English translator will be provided at this meeting. All... External Affairs, at (866) 208-FERC, or on the FERC Web site at www.ferc.gov using the ``eLibrary'' link...

  10. Cytochrome P450-mediated activation of the fragrance compound geraniol forms potent contact allergens

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hagvall, Lina; Baron, Jens Malte; Boerje, Anna

    2008-12-01

    Contact sensitization is caused by low molecular weight compounds which penetrate the skin and bind to protein. In many cases, these compounds are activated to reactive species, either by autoxidation on exposure to air or by metabolic activation in the skin. Geraniol, a widely used fragrance chemical, is considered to be a weak allergen, although its chemical structure does not indicate it to be a contact sensitizer. We have shown that geraniol autoxidizes and forms allergenic oxidation products. In the literature, it is suggested but not shown that geraniol could be metabolically activated to geranial. Previously, a skin-like CYP cocktailmore » consisting of cutaneous CYP isoenzymes, was developed as a model system to study cutaneous metabolism. In the present study, we used this system to investigate CYP-mediated activation of geraniol. In incubations with the skin-like CYP cocktail, geranial, neral, 2,3-epoxygeraniol, 6,7-epoxygeraniol and 6,7-epoxygeranial were identified. Geranial was the main metabolite formed followed by 6,7-epoxygeraniol. The allergenic activities of the identified metabolites were determined in the murine local lymph node assay (LLNA). Geranial, neral and 6,7-epoxygeraniol were shown to be moderate sensitizers, and 6,7-epoxygeranial a strong sensitizer. Of the isoenzymes studied, CYP2B6, CYP1A1 and CYP3A5 showed high activities. It is likely that CYP1A1 and CYP3A5 are mainly responsible for the metabolic activation of geraniol in the skin, as they are expressed constitutively at significantly higher levels than CYP2B6. Thus, geraniol is activated through both autoxidation and metabolism. The allergens geranial and neral are formed via both oxidation mechanisms, thereby playing a large role in the sensitization to geraniol.« less

  11. Cytochrome P450-mediated activation of the fragrance compound geraniol forms potent contact allergens.

    PubMed

    Hagvall, Lina; Baron, Jens Malte; Börje, Anna; Weidolf, Lars; Merk, Hans; Karlberg, Ann-Therese

    2008-12-01

    Contact sensitization is caused by low molecular weight compounds which penetrate the skin and bind to protein. In many cases, these compounds are activated to reactive species, either by autoxidation on exposure to air or by metabolic activation in the skin. Geraniol, a widely used fragrance chemical, is considered to be a weak allergen, although its chemical structure does not indicate it to be a contact sensitizer. We have shown that geraniol autoxidizes and forms allergenic oxidation products. In the literature, it is suggested but not shown that geraniol could be metabolically activated to geranial. Previously, a skin-like CYP cocktail consisting of cutaneous CYP isoenzymes, was developed as a model system to study cutaneous metabolism. In the present study, we used this system to investigate CYP-mediated activation of geraniol. In incubations with the skin-like CYP cocktail, geranial, neral, 2,3-epoxygeraniol, 6,7-epoxygeraniol and 6,7-epoxygeranial were identified. Geranial was the main metabolite formed followed by 6,7-epoxygeraniol. The allergenic activities of the identified metabolites were determined in the murine local lymph node assay (LLNA). Geranial, neral and 6,7-epoxygeraniol were shown to be moderate sensitizers, and 6,7-epoxygeranial a strong sensitizer. Of the isoenzymes studied, CYP2B6, CYP1A1 and CYP3A5 showed high activities. It is likely that CYP1A1 and CYP3A5 are mainly responsible for the metabolic activation of geraniol in the skin, as they are expressed constitutively at significantly higher levels than CYP2B6. Thus, geraniol is activated through both autoxidation and metabolism. The allergens geranial and neral are formed via both oxidation mechanisms, thereby playing a large role in the sensitization to geraniol.

  12. One-step purification of a functional, constitutively activated form of visual arrestin.

    PubMed

    Huang, Li; Mao, Xiang; Abdulaev, Najmoutin G; Ngo, Tony; Liu, Wei; Ridge, Kevin D

    2012-03-01

    Desensitization of agonist-activated G protein-coupled receptors (GPCRs) requires phosphorylation followed by the binding of arrestin, a ~48 kDa soluble protein. While crystal structures for the inactive, 'basal' state of various arrestins are available, the conformation of 'activated' arrestin adopted upon interaction with activated GPCRs remains unknown. As a first step towards applying high-resolution structural methods to study arrestin conformation and dynamics, we have utilized the subtilisin prodomain/Profinity eXact™ fusion-tag system for the high-level bacterial expression and one-step purification of wild-type visual arrestin (arrestin 1) as well as a mutant form (R175E) of the protein that binds to non-phosphorylated, light-activated rhodopsin (Rho∗). The results show that both prodomain/Profinity eXact™ fusion-tagged wild-type and R175E arrestins can be expressed to levels approaching 2-3 mg/l in Luria-Bertani media, and that the processed, tag-free mature forms can be purified to near homogeneity using a Bio-Scale™ Mini Profinity eXact™ cartridge on the Profinia™ purification system. Functional analysis of R175E arrestin generated using this approach shows that it binds to non-phosphorylated rhodopsin in a light-dependent manner. These findings should facilitate the structure determination of this 'constitutively activated' state of arrestin 1 as well as the monitoring of conformational changes upon interaction with Rho∗. Copyright © 2011 Elsevier Inc. All rights reserved.

  13. 76 FR 9805 - Agency Information Collection Activities: Form G-845 and Supplement; Revision of a Currently...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-22

    ... Collection Activities: Form G-845 and Supplement; Revision of a Currently Approved Information Collection; Comment Request ACTION: 60-Day Notice of Information Collection Under Review: Form G- 845 and Supplement... other forms of information technology, e.g., permitting electronic submission of responses. Overview of...

  14. 77 FR 19279 - Northern Indiana Public Service Company; Notice of Petition for Declaratory Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ... Transmission Investment Through Pricing Reform, Order No. 679, FERC Stats. & Regs. ] 31,222, order on reh'g, Order No. 679-A, FERC Stats. & Regs. ] 3l 31,236 (2006), order on reh'g, 119FERC ] 61,062 (2007). Any...

  15. 78 FR 62644 - Agency Information Collection Activities: Application for Temporary Protected Status, Form I-821...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ... DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services [OMB Control Number 1615-0043] Agency Information Collection Activities: Application for Temporary Protected Status, Form I-821... Currently Approved Collection. (2) Title of the Form/Collection: Application for Temporary Protected Status...

  16. Pore-forming activity and structural autoinhibition of the gasdermin family.

    PubMed

    Ding, Jingjin; Wang, Kun; Liu, Wang; She, Yang; Sun, Qi; Shi, Jianjin; Sun, Hanzi; Wang, Da-Cheng; Shao, Feng

    2016-07-07

    Inflammatory caspases cleave the gasdermin D (GSDMD) protein to trigger pyroptosis, a lytic form of cell death that is crucial for immune defences and diseases. GSDMD contains a functionally important gasdermin-N domain that is shared in the gasdermin family. The functional mechanism of action of gasdermin proteins is unknown. Here we show that the gasdermin-N domains of the gasdermin proteins GSDMD, GSDMA3 and GSDMA can bind membrane lipids, phosphoinositides and cardiolipin, and exhibit membrane-disrupting cytotoxicity in mammalian cells and artificially transformed bacteria. Gasdermin-N moved to the plasma membrane during pyroptosis. Purified gasdermin-N efficiently lysed phosphoinositide/cardiolipin-containing liposomes and formed pores on membranes made of artificial or natural phospholipid mixtures. Most gasdermin pores had an inner diameter of 10–14 nm and contained 16 symmetric protomers. The crystal structure of GSDMA3 showed an autoinhibited two-domain architecture that is conserved in the gasdermin family. Structure-guided mutagenesis demonstrated that the liposome-leakage and pore-forming activities of the gasdermin-N domain are required for pyroptosis. These findings reveal the mechanism for pyroptosis and provide insights into the roles of the gasdermin family in necrosis, immunity and diseases.

  17. 76 FR 30342 - Gulf South Pipeline Company, LP; Notice of Request for Waiver

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... Conduct for Transmission Providers, FERC Stats. & Regs. 31,280 (2008) (Order No 717), order on reh'g, FERC Stats. & Regs. ] 31,297 (2009) (Order No. 717-A, order on reh'g, 129 FERC ]61,123 (2009) (Order No. 717B...

  18. 76 FR 9810 - Agency Information Collection Activities: Comment Request for the Ferrous Metals Surveys (17 Forms)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-22

    ... Activities: Comment Request for the Ferrous Metals Surveys (17 Forms) AGENCY: U.S. Geological Survey (USGS... with domestic consumption data of 13 ores, concentrates, metals, and ferroalloys, some of which are...-0068. Form Number: Various (17 forms). Title: Ferrous Metals Surveys. Type of Request: Extension of a...

  19. 75 FR 5097 - Agency Information Collection Activities: Form G-646, Extension of a Currently Approved...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Collection Activities: Form G-646, Extension of a Currently Approved Information Collection; Comment Request ACTION: 30-day Notice of Information Collection Under Review: Form G- 646, Sworn Statement of Refugee... information technology, e.g., permitting electronic submission of responses. Overview of This information...

  20. A Natural Chimeric Pseudomonas Bacteriocin with Novel Pore-Forming Activity Parasitizes the Ferrichrome Transporter.

    PubMed

    Ghequire, Maarten G K; Kemland, Lieselore; Anoz-Carbonell, Ernesto; Buchanan, Susan K; De Mot, René

    2017-02-21

    Modular bacteriocins represent a major group of secreted protein toxins with a narrow spectrum of activity, involved in interference competition between Gram-negative bacteria. These antibacterial proteins include a domain for binding to the target cell and a toxin module at the carboxy terminus. Self-inhibition of producers is provided by coexpression of linked immunity genes that transiently inhibit the toxin's activity through formation of bacteriocin-immunity complexes or by insertion in the inner membrane, depending on the type of toxin module. We demonstrate strain-specific inhibitory activity for PmnH, a Pseudomonas bacteriocin with an unprecedented dual-toxin architecture, hosting both a colicin M domain, potentially interfering with peptidoglycan synthesis, and a novel colicin N-type domain, a pore-forming module distinct from the colicin Ia-type domain in Pseudomonas aeruginosa pyocin S5. A downstream-linked gene product confers PmnH immunity upon susceptible strains. This protein, ImnH, has a transmembrane topology similar to that of Pseudomonas colicin M-like and pore-forming immunity proteins, although homology with either of these is essentially absent. The enhanced killing activity of PmnH under iron-limited growth conditions reflects parasitism of the ferrichrome-type transporter for entry into target cells, a strategy shown here to be used as well by monodomain colicin M-like bacteriocins from pseudomonads. The integration of a second type of toxin module in a bacteriocin gene could offer a competitive advantage against bacteria displaying immunity against only one of both toxic activities. IMPORTANCE In their continuous struggle for ecological space, bacteria face a huge load of contenders, including phylogenetically related strains that compete for the same niche. One important group of secreted antibacterial proteins assisting in eliminating these rivals are modular bacteriocins of Gram-negative bacteria, comprising a domain for docking onto the

  1. Integration of active pharmaceutical ingredient solid form selection and particle engineering into drug product design.

    PubMed

    Ticehurst, Martyn David; Marziano, Ivan

    2015-06-01

    This review seeks to offer a broad perspective that encompasses an understanding of the drug product attributes affected by active pharmaceutical ingredient (API) physical properties, their link to solid form selection and the role of particle engineering. While the crucial role of active pharmaceutical ingredient (API) solid form selection is universally acknowledged in the pharmaceutical industry, the value of increasing effort to understanding the link between solid form, API physical properties and drug product formulation and manufacture is now also being recognised. A truly holistic strategy for drug product development should focus on connecting solid form selection, particle engineering and formulation design to both exploit opportunities to access simpler manufacturing operations and prevent failures. Modelling and predictive tools that assist in establishing these links early in product development are discussed. In addition, the potential for differences between the ingoing API physical properties and those in the final product caused by drug product processing is considered. The focus of this review is on oral solid dosage forms and dry powder inhaler products for lung delivery. © 2015 Royal Pharmaceutical Society.

  2. 75 FR 5099 - Agency Information Collection Activities: Visa Waiver Program Carrier Agreement (Form I-775)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Activities: Visa Waiver Program Carrier Agreement (Form I-775) AGENCY: U.S. Customs and Border Protection... and Budget (OMB) for review and approval in accordance with the Paperwork Reduction Act: Visa Waiver... other technological techniques or other forms of information. Title: Visa Waiver Program Carrier...

  3. 76 FR 48874 - Agency Information Collection Activities: Form G-884, Extension of an Existing Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... Activities: Form G-884, Extension of an Existing Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form G- 884, Request for the Return of Original Document(s... information technology, e.g., permitting electronic submission of responses. Overview of This Information...

  4. Special Form Testing of Sealed Source Encapsulation for High-Alpha-Activity Actinide Materials

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Martinez, Oscar A

    In the United States all transportation of radioactive material is regulated by the U.S. Department of Transportation (DOT). Beginning in 2008 a new type of sealed-source encapsulation package was developed and tested by Oak Ridge National Laboratory (ORNL). These packages contain high-alpha-activity actinides and are regulated and transported in accordance with the requirements for DOT Class 7 hazardous material. The DOT provides specific regulations pertaining to special form encapsulation designs. The special form designation indicates that the encapsulated radioactive contents have a very low probability of dispersion even when subjected to significant structural events. The special form designs have beenmore » shown to simplify the delivery, transport, acceptance, and receipt processes. It is intended for these sealed-source encapsulations to be shipped to various facilities making it very advantageous for them to be certified as special form. To this end, DOT Certificates of Competent Authority (CoCAs) have been sought for the design suitable for containing high-alpha-activity actinide materials. This design consists of the high-alpha-activity material encapsulated within a triangular zirconia canister, referred to as a ZipCan, tile that is then enclosed by a spherical shell. The spherical shell design, with ZipCan tile inside, was tested for compliance with the special form regulations found in 49 CFR 173.469. The spherical enclosure was subjected to 9-m impact, 1 m percussion, and 10-minute thermal tests at the Packaging Evaluation Facility located at the National Transportation Research Center in Knoxville, TN USA and operated by ORNL. Before and after each test, the test units were subjected to a helium leak check and a bubble test. The ZipCan tiles and core were also subjected to the tests required for ISO 2919:2012(E), including a Class IV impact test and heat test and subsequently subjected to helium leakage rate tests [49 CFR 173.469(a)(4)(i)]. The

  5. 77 FR 43345 - Agency Information Collection Activities: Sponsor's Notice of Change of Address, Form I-865...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-24

    ...-0076] Agency Information Collection Activities: Sponsor's Notice of Change of Address, Form I-865...: Sponsor's Notice of Change of Address. (3) Agency form number, if any, and the applicable component of the.... During this 60-day period, USCIS will be evaluating whether to revise the Form I-865. Should USCIS decide...

  6. Expression, purification and characterization of inactive and active forms of ERK2 from insect expression system.

    PubMed

    Yan, Kelly; Merritt, Hanne; Crawford, Kenneth; Pardee, Gwynn; Cheng, Jan Marie; Widger, Stephania; Hekmat-Nejad, Mohammad; Zaror, Isabel; Sim, Janet

    2015-06-01

    Extracellular signal-regulated kinase 2 (ERK2) is a serine/threonine protein kinase involved in many cellular programs, such as cell proliferation, differentiation, motility and programed cell-death. It is therefore considered an important target in the treatment of cancer. In an effort to support biochemical screening and small molecule drug discovery, we established a robust system to generate both inactive and active forms of ERK2 using insect expression system. We report here, for the first time, that inactive ERK2 can be expressed and purified with 100% homogeneity in the unphosphorylated form using insect system. This resulted in a significant 20-fold yield improvement compared to that previously reported using bacterial expression system. We also report a newly developed system to generate active ERK2 in insect cells through in vivo co-expression with a constitutively active MEK1 (S218D S222D). Isolated active ERK2 was confirmed to be doubly phosphorylated at the correct sites, T185 and Y187, in the activation loop of ERK2. Both ERK2 forms, inactive and active, were well characterized by biochemical activity assay for their kinase function. Inactive and active ERK2 were the two key reagents that enabled successful high through-put biochemical assay screen and structural drug discovery studies. Copyright © 2015 Elsevier Inc. All rights reserved.

  7. 75 FR 22125 - Trans-Union Interstate Pipeline, L.P.; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-27

    ... Interstate Pipeline, L.P.; Notice of Filing April 20, 2010. Take notice that on April 9, 2010 Trans-Union Interstate Pipeline, L.P. submitted a request for waiver of the requirement to submit the 2009 FERC Form No... am] BILLING CODE 6717-01-P ...

  8. Neuroserpin Differentiates Between Forms of Tissue Type Plasminogen Activator via pH Dependent Deacylation

    PubMed Central

    Carlson, Karen-Sue B.; Nguyen, Lan; Schwartz, Kat; Lawrence, Daniel A.; Schwartz, Bradford S.

    2016-01-01

    Tissue-type plasminogen activator (t-PA), initially characterized for its critical role in fibrinolysis, also has key functions in both physiologic and pathologic processes in the CNS. Neuroserpin (NSP) is a t-PA specific serine protease inhibitor (serpin) found almost exclusively in the CNS that regulates t-PA’s proteolytic activity and protects against t-PA mediated seizure propagation and blood–brain barrier disruption. This report demonstrates that NSP inhibition of t-PA varies profoundly as a function of pH within the biologically relevant pH range for the CNS, and reflects the stability, rather than the formation of NSP: t-PA acyl-enzyme complexes. Moreover, NSP differentiates between the zymogen-like single chain form (single chain t-PA, sct-PA) and the mature protease form (two chain t-PA, tct-PA) of t-PA, demonstrating different pH profiles for protease inhibition, different pH ranges over which catalytic deacylation occurs, and different pH dependent profiles of deacylation rates for each form of t-PA. NSP’s pH dependent inhibition of t-PA is not accounted for by differential acylation, and is specific for the NSP-t-PA serpin-protease pair. These results demonstrate a novel mechanism for the differential regulation of the two forms of t-PA in the CNS, and suggest a potential specific regulatory role for CNS pH in controlling t-PA proteolytic activity. PMID:27378851

  9. 78 FR 21929 - Transmission Relay Loadability Reliability Standard; Notice of Compliance Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-12

    ... Relay Loadability Reliability Standard; Notice of Compliance Filing Take notice that on February 19... Relay Loadability Reliability Standard, Order No. 733, 130 FERC ] 61,221 (2010) (Order No. 733); order..., 136 FERC ] 61,185 (2011). \\2\\ Transmission Relay Loadability Reliability Standard, 138 FERC ] 61,197...

  10. 78 FR 5477 - Agency Information Collection Activities: InfoPass System, No Form Number; Extension, Without...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-25

    ...-0113] Agency Information Collection Activities: InfoPass System, No Form Number; Extension, Without... Change, of a Currently Approved Collection. (2) Title of the Form/Collection: InfoPass System. (3) Agency...: Primary: Individuals or households. The InfoPass system allows an applicant or petitioner to schedule an...

  11. Membrane Treatment of Aqueous Film Forming Foam (AFFF) Wastes for Recovery of Its Active Ingredients

    DTIC Science & Technology

    1980-10-01

    T ME1MBRANE TREATMENT OF AQUEOUS FILM FORMING FOAM~ (AFFF) WASTES FOR RECOVERY OFI Fts ACTIVE INGREDIENTS FINAL REPORT October 1980 by Edward S. K...OF THIS PAGEOPMn Date AVntr* d)__ ---- Ultrafiltration (UF) and Reverse Osmosis (RO) treatment of Aqueous Film Forming Foam (AFFF) solutions was...of Aqueous Film Forming Foam (AFFF) solutions was investigated to determine the feasibility of employing membrane processes to separate and recover

  12. Structure of the cleavage-activated prefusion form of the parainfluenza virus 5 fusion protein.

    PubMed

    Welch, Brett D; Liu, Yuanyuan; Kors, Christopher A; Leser, George P; Jardetzky, Theodore S; Lamb, Robert A

    2012-10-09

    The paramyxovirus parainfluenza virus 5 (PIV5) enters cells by fusion of the viral envelope with the plasma membrane through the concerted action of the fusion (F) protein and the receptor binding protein hemagglutinin-neuraminidase. The F protein folds initially to form a trimeric metastable prefusion form that is triggered to undergo large-scale irreversible conformational changes to form the trimeric postfusion conformation. It is thought that F refolding couples the energy released with membrane fusion. The F protein is synthesized as a precursor (F0) that must be cleaved by a host protease to form a biologically active molecule, F1,F2. Cleavage of F protein is a prerequisite for fusion and virus infectivity. Cleavage creates a new N terminus on F1 that contains a hydrophobic region, known as the FP, which intercalates target membranes during F protein refolding. The crystal structure of the soluble ectodomain of the uncleaved form of PIV5 F is known; here we report the crystal structure of the cleavage-activated prefusion form of PIV5 F. The structure shows minimal movement of the residues adjacent to the protease cleavage site. Most of the hydrophobic FP residues are buried in the uncleaved F protein, and only F103 at the newly created N terminus becomes more solvent-accessible after cleavage. The conformational freedom of the charged arginine residues that compose the protease recognition site increases on cleavage of F protein.

  13. Structure of the cleavage-activated prefusion form of the parainfluenza virus 5 fusion protein

    PubMed Central

    Welch, Brett D.; Liu, Yuanyuan; Kors, Christopher A.; Leser, George P.; Jardetzky, Theodore S.; Lamb, Robert A.

    2012-01-01

    The paramyxovirus parainfluenza virus 5 (PIV5) enters cells by fusion of the viral envelope with the plasma membrane through the concerted action of the fusion (F) protein and the receptor binding protein hemagglutinin-neuraminidase. The F protein folds initially to form a trimeric metastable prefusion form that is triggered to undergo large-scale irreversible conformational changes to form the trimeric postfusion conformation. It is thought that F refolding couples the energy released with membrane fusion. The F protein is synthesized as a precursor (F0) that must be cleaved by a host protease to form a biologically active molecule, F1,F2. Cleavage of F protein is a prerequisite for fusion and virus infectivity. Cleavage creates a new N terminus on F1 that contains a hydrophobic region, known as the FP, which intercalates target membranes during F protein refolding. The crystal structure of the soluble ectodomain of the uncleaved form of PIV5 F is known; here we report the crystal structure of the cleavage-activated prefusion form of PIV5 F. The structure shows minimal movement of the residues adjacent to the protease cleavage site. Most of the hydrophobic FP residues are buried in the uncleaved F protein, and only F103 at the newly created N terminus becomes more solvent-accessible after cleavage. The conformational freedom of the charged arginine residues that compose the protease recognition site increases on cleavage of F protein. PMID:23012473

  14. [Morphological signs of inflammatory activity in different clinical forms of drug-resistant pulmonary tuberculosis].

    PubMed

    Elipashev, A A; Nikolsky, V O; Shprykov, A S

    to determine whether the activity of tuberculous inflammation is associated with different clinical forms of drug-resistant pulmonary tuberculosis. The material taken from 310 patients operated on in 2010-2015 were retrospectively examined. The patients underwent economical lung resections of limited extent (typical and atypical ones of up to 3 segments) for circumscribed forms of tuberculosis with bacterial excretion. A study group consisted of 161 (51.9%) patients with drug-resistant variants of pulmonary tuberculosis. A control group included 149 (48.1%) patients with preserved susceptibility of Mycobacterium tuberculosis to anti-TB drugs. The activity of specific changes in tuberculosis was morphologically evaluated in accordance with the classification proposed by B.M. Ariel in 1998. The highest activity of fourth-to-fifth degree specific inflammation, including that outside the primary involvement focus, was obtained in the drug-resistant pulmonary tuberculosis group due to the predominance of patients with cavernous and fibrous-cavernous tuberculosis versus those in whom the susceptibility to chemotherapeutic agents was preserved. A macroscopic study showed that the primary lesion focus had a median size in one-half of the all the examinees; but large tuberculomas, caverns, and fibrous caverns over 4 cm in diameter were multiple and detected in the drug-resistant pulmonary tuberculosis group. Multidrug resistance was observed in more than 60% of the patients with fibrous-cavernous pulmonary tuberculosis, extensive drug resistance was seen in those with cavernous tuberculosis, which is an aggravating factor. The data obtained from the morphological study of the intraoperative material can specify the clinical form of tuberculosis and evaluate the efficiency of preoperative specific therapy. The highest activity of specific inflammation was observed in patients with multiple drug-resistant pulmonary tuberculosis, the prevalence of third-to-fourth degree

  15. Effects of Three Forms of Reading-Based Output Activity on L2 Vocabulary Learning

    ERIC Educational Resources Information Center

    Rassaei, Ehsan

    2017-01-01

    The current study investigated the effects of three forms of output activity on EFL learners' recognition and recall of second language (L2) vocabulary. To this end, three groups of learners of English as a foreign language (EFL) were instructed to employ the following three output activities after reading two narrative texts: (1) summarizing the…

  16. An Aqueous Extract of Marine Microalgae Exhibits Antimetastatic Activity through Preferential Killing of Suspended Cancer Cells and Anticolony Forming Activity

    PubMed Central

    Somasekharan, Syam Prakash; El-Naggar, Amal; Sorensen, Poul H.

    2016-01-01

    Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma. In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells. In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted. PMID:27656243

  17. 76 FR 12094 - Whitman River Dam, Inc.

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    [email protected]net . i. FERC Contact: Jeff Browning, (202) 502-8677 or [email protected] . j. Cooperating....ferc.gov/docs-filing/ferconline.asp ). Commenters can submit brief comments up to 6,000 characters, without prior registration, using the eComment system at http://www.ferc.gov/docs-filing/ecomment.asp...

  18. 75 FR 5781 - David Noland; Notice of Declaration of Intention and Soliciting Comments, Protests, and/or...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-04

    ...: //[email protected]net . i. FERC Contact: Any questions on this notice should be addressed to... filings, please go to the Commission's Web site located at http://www.ferc.gov .filing-comments.asp... access the document. You may also register online at http://www.ferc.gov/docs-filing/esubscription.asp to...

  19. 18 CFR 131.31 - FERC Form No. 561, Annual report of interlocking positions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... supplies electric equipment (ELEQ) named in Column (3) enter the aggregate amount of revenues from... utility ELEQ Entity which produces/supplies electric equipment for the use of any public utility FUEL Entity which produces/supplies coal, natural gas, nuclear fuel, or other fuel for the use of any public...

  20. 78 FR 73875 - Agency Information Collection Activities: Canadian Border Boat Landing Permit (CBP Form I-68)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-09

    ... Activities: Canadian Border Boat Landing Permit (CBP Form I-68) AGENCY: U.S. Customs and Border Protection... information collection requirement concerning the Canadian Border Boat Landing Permit (Form I-68). This... soliciting comments concerning the following information collection: Title: Canadian Border Boat Landing...

  1. [Active form of vitamin D in overweight and obese pediatric patients in northwest Mexico].

    PubMed

    Valle-Leal, Jaime; Limón-Armenta, Jasmin; Serrano-Osuna, Ricardo; López-Morales, Cruz Mónica; Alvárez-Bastidas, Lucia

    Low levels of vitamin D have been associated with a range of clinical conditions such as obesity, insulin resistance, and diabetes mellitus, among others. There are few studies that measure the active form of vitamin D (1,25 (OH) 2 vitamin D) in obese children. However, published data are inconclusive. The aim of this study was to determine the active levels of vitamin D in obese and overweight children and to find an association between low levels of vitamin D, obesity and impaired glucose metabolism. A cross-sectional, analytical study was conducted in 6 to 12-year-old children with excess adiposity determined by waist-stature index and body mass index. Levels of glucose, insulin, complete lipid profile, homeostatic model assessment and the active form of vitamin D were measured in each patient. Levels < 30 pg/ml were considered as low levels of vitamin D. The prevalence of low levels of active vitamin D was 36%. A significant association between low levels of active vitamin D and high levels of insulin was found. No significant association was found between vitamin levels and adiposity measures. Low levels of active vitamin D were found in 36% of the population studied. A significant association with insulin resistance and hyperinsulinemia was demonstrated. Copyright © 2017 Hospital Infantil de México Federico Gómez. Publicado por Masson Doyma México S.A. All rights reserved.

  2. Development of active CFRP/metal laminates and their demonstrations in complicated forms

    NASA Astrophysics Data System (ADS)

    Asanuma, H.; Nakata, T.; Tanaka, T.; Imori, M.; Haga, O.

    2006-03-01

    This paper describes development of high performance CFRP/metal active laminates and demonstrations of them in complicated forms. Various types of the laminates were made by hot-pressing of an aluminum, aluminum alloys, a stainless steel and a titanium for the metal layer as a high CTE material, a unidirectional CFRP prepreg as a low CTE/electric resistance heating material, a unidirectional KFRP prepreg as a low CTE/insulating material. The aluminum and its alloy type laminates have almost the same and the highest room temperature curvatures and they linearly change with increasing temperature up to their fabrication temperature. The curvature of the stainless steel type jumps from one to another around its fabrication temperature, whereas the titanium type causes a double curvature and its change becomes complicated. The output force of the stainless steel type attains the highest of the three under the same thickness. The aluminum type successfully increased its output force by increasing its thickness and using its alloys. The electric resistance of the CFRP layer can be used to monitor the temperature, that is, the curvature of the active laminate because the curvature is a function of temperature. The aluminum type active laminate was made into complicated forms, that is, a hatch, a stack, a coil and a lift types, and their actuation performances were successfully demonstrated.

  3. 77 FR 27767 - Milford Hydro, LLC; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... Contact: Bryan Roden-Reynolds at (202) 502-6618, or via email at bryan.roden-reynolds@ferc.gov . Deadline... site at http://www.ferc.gov/docs-filing/elibrary.asp . Enter the docket number (P-14218) in the docket number field to access the document. For assistance, contact FERC Online Support. Dated: May 4, 2012...

  4. 78 FR 60271 - Hollow Dam Power Company; Ampersand Hollow Dam Hydro, LLC; Notice of Application for Transfer of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-01

    ...Library link of Commission's Web site at http://www.ferc.gov/docs-filing/elibrary.asp . Enter the docket...) and the instructions on the Commission's Web site under http://www.ferc.gov/docs-filing/efiling.asp... system at http://www.ferc.gov/docs-filing/ecomment.asp . You must include your name and contact...

  5. 78 FR 38027 - Wilkesboro Hydroelectric Company, LLC; Wilkesboro Hydropower, LLC; Notice of Application for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-25

    ... viewed or printed on the eLibrary link of Commission's Web site at http://www.ferc.gov/docs-filing... Commission's Web site under http://www.ferc.gov/docs-filing/efiling.asp . Commenters can submit brief comments up to 6,000 characters, without prior registration, using the eComment system at http://www.ferc...

  6. 78 FR 25432 - Sabine Pass LNG, L.P., Sabine Pass Liquefaction, LLC; Notice of Availability of the Environmental...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... Affairs, at (866) 208-FERC, or on the FERC Web site ( www.ferc.gov ) using the eLibrary link. Click on the... heavies removal column, a heavies removal column reboiler, a debutanizer system, and a condensate stabilizer system to be located inside the battery limits within each of the four liquefaction trains; two...

  7. 78 FR 934 - 37 Wilton Road, Milford LLC, and 282 Route 101 LLC, PVM Commercial Center, LLC; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-07

    ... printed on the eLibrary link of Commission's Web site at http://www.ferc.gov/docs-filing/elibrary.asp... Commission's Web site under http://www.ferc.gov/docs-filing/efiling.asp . Commenters can submit brief comments up to 6,000 characters, without prior registration, using the eComment system at http://www.ferc...

  8. 75 FR 9199 - Combined Notice of Filings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-01

    ...Mobil Gas & Power Marketing Company and amendments to the Service Agreements. Filed Date: 02/19/2010... of protests and interventions in lieu of paper, using the FERC Online links at http://www.ferc.gov... notification when a document is added to a subscribed docket(s). For assistance with any FERC Online service...

  9. 77 FR 74487 - Agency Information Collection Activities: Petition for Alien Fiance(e), Form Number I-129F...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-14

    ...-0001] Agency Information Collection Activities: Petition for Alien Fiance(e), Form Number I-129F... information collection. (2) Title of the Form/Collection: Petition for Alien Fiance(e). (3) Agency form number... petition for an alien fiance(e), spouse, or his/her children. (5) An estimate of the total number of...

  10. 78 FR 19726 - Agency Information Collection Activities: Visa Waiver Program Carrier Agreement (CBP Form I-775)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-02

    ... Activities: Visa Waiver Program Carrier Agreement (CBP Form I-775) AGENCY: U.S. Customs and Border Protection... information collection requirement concerning the Visa Waiver Program Carrier Agreement (CBP Form I-775). This... concerning the following information collection: Title: Visa Waiver Program Carrier Agreement. OMB Number...

  11. 77 FR 12071 - Agency Information Collection Activities: Form G-28, Revision of a Currently Approved Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... Collection Activities: Form G-28, Revision of a Currently Approved Information Collection; Comment Request ACTION: 30-Day Notice of Information Collection Under Review: Form G- 28, Notice of Entry of Appearance... technology, e.g., permitting electronic submission of responses. Overview of This information collection: (1...

  12. 76 FR 77223 - Enbridge Offshore Pipelines (UTOS); Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-12

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC12-20-000] Enbridge Offshore Pipelines (UTOS); Notice of Filing Take notice that on November 30, 2011, Enbridge Offshore Pipelines (UTOS) submitted a request for a waiver of the reporting requirement to file the FERC Form 2 CPA...

  13. 12 CFR Appendix to Part 1253 - Prior Approval for Enterprise Products-Instructions and Notice of New Activity Form

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Approval for Enterprise Products—Instructions and Notice of New Activity Form ER02JY09.000 ER02JY09.001... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Prior Approval for Enterprise Products-Instructions and Notice of New Activity Form Appendix to Part 1253 Banks and Banking FEDERAL HOUSING FINANCE...

  14. 75 FR 10241 - Town of Dexter; Notice of Declaration of Intention and Soliciting Comments, Protests, and/or...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-05

    ..., ME 04930; telephone: (207) 924-0180; e-mail: http: //[email protected]net . i. FERC Contact... site at http://www.ferc.gov .filing- comments.asp. Please include the docket number (DI10-7-000) on any... document. You may also register online at http://www.ferc.gov/docs-filing/esubscription.asp to be notified...

  15. 75 FR 67959 - Wagon Wheel Associates; Notice of Application Tendered for Filing With the Commission, Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-04

    ....5 Goose Creek Road, South Fork, CO 81154; (970) 925-6071; [email protected]net . j. FERC Contact...). Register online at http://www.ferc.gov/docs-filing/esubscription.asp to be notified via e-mail of new... on the Commission's Web site ( http://www.ferc.gov/docs-filing/ferconline.asp ) under the ``e- Filing...

  16. 75 FR 43965 - Texas Eastern Transmission, LP; Algonquin Gas Transmission, LLC; Notice of Intent To Prepare an...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-27

    ... prepared by the FERC entitled ``An Interstate Natural Gas Facility on My Land? What Do I Need To Know?'' is available for viewing on the FERC Web site ( http://www.ferc.gov ). This fact sheet addresses a number of... metering and regulating (M&R) station in the Borough of Staten Island, New York; Construction of...

  17. 78 FR 14528 - Mayo Hydropower, LLC, Avalon Hydropower, LLC; Notice of Application for Transfer of License, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-06

    ... this project can be viewed or printed on the eLibrary link of Commission's Web site at http://www.ferc...) and the instructions on the Commission's Web site under http://www.ferc.gov/docs-filing/efiling.asp... system at http://www.ferc.gov/docs-filing/ecomment.asp . You must include your name and contact...

  18. 76 FR 12958 - Amnor Hydro West Inc.; Notice of Preliminary Permit Application Accepted for Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-09

    ... http:[sol][sol]www.ferc.gov/docs-filing/efiling.asp. Commenters can submit brief comments up to 6,000 characters, without prior registration, using the eComment system at http:[sol][sol]www.ferc.gov/docs-filing...Library'' link of Commission's Web site at http:[sol][sol]www.ferc.gov/docs-filing/ elibrary.asp. Enter...

  19. 78 FR 5477 - Agency Information Collection Activities: Inter-Agency Alien Witness and Informant Record, Form I...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-25

    ...-0046] Agency Information Collection Activities: Inter-Agency Alien Witness and Informant Record, Form I... collection. (2) Title of the Form/Collection: Inter-Agency Alien Witness and Informant Record. (3) Agency...- 854 is used by law enforcement agencies to bring alien witnesses and informants to the United States...

  20. 78 FR 9049 - Agency Information Collection Activities: Proposed Collection Renewal; Comment Request Re Forms...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-07

    ... FEDERAL DEPOSIT INSURANCE CORPORATION Agency Information Collection Activities: Proposed Collection Renewal; Comment Request Re Forms Relating to Processing Deposit Insurance Claims AGENCY: Federal Deposit Insurance Corporation (FDIC). ACTION: Notice of proposed information collection renewal and...

  1. 75 FR 81152 - Interpretation of Protection System Reliability Standard

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-27

    ... created electronically using word processing software should be filed in native applications or print-to... reh'g & compliance, 117 FERC ] 61,126 (2006), aff'd sub nom. Alcoa, Inc. v. FERC, 564 F.3d 1342 (DC... print-to-PDF format and not in a scanned format, at http://www.ferc.gov/docs-filing/efiling.asp . Mail...

  2. 77 FR 7526 - Interpretation of Protection System Reliability Standard

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-13

    ... reh'g & compliance, 117 FERC ] 61,126 (2006), aff'd sub nom. Alcoa, Inc. v. FERC, 564 F.3d 1342 (D.C... opportunity to view and/or print the contents of this document via the Internet through FERC's Home Page... available on eLibrary in PDF and Microsoft Word format for viewing, printing, and/or downloading. To access...

  3. 76 FR 58101 - Electric Reliability Organization Interpretation of Transmission Operations Reliability Standard

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-20

    ... on reh'g & compliance, 117 FERC ] 61,126 (2006), aff'd sub nom. Alcoa, Inc. v. FERC, 564 F.3d 1342 (D... persons an opportunity to view and/or print the contents of this document via the Internet through FERC's... document is available on eLibrary in PDF and Microsoft Word format for viewing, printing, and/or...

  4. 78 FR 78349 - Andrew Peklo III; Notice of Availability of Draft Environmental Assessment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-26

    ... draft EA may also be viewed on the Commission's Web site at http://www.ferc.gov using the ``eLibrary... CFR 385.2001(a)(1)(iii) and the instructions on the Commission's Web site http://www.ferc.gov/docs... registration, using the eComment system at http://www.ferc.gov/docs-filing/ecomment.asp . You must include your...

  5. 77 FR 277 - Sabine Pass Liquefaction, LLC and Sabine Pass LNG, L.P; Notice of Availability of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-04

    ... Web site ( www.ferc.gov ) using the eLibrary link. A limited number of copies of the EA are available..., or on the FERC Web site ( www.ferc.gov ) using the eLibrary link. Click on the eLibrary link, click... systems, induced draft air coolers, fire and gas detection and safety systems, control systems, and...

  6. 77 FR 21761 - Alice Falls Corporation, Alice Falls Hydro, LLC; Notice of Application for Transfer of License...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... under http:[sol][sol]www.ferc.gov/docs-filing/efiling.asp. Commenters can submit brief comments up to 6,000 characters, without prior registration, using the eComment system at http:[sol][sol]www.ferc.gov... printed on the eLibrary link of Commission's Web site at http:[sol][sol]www.ferc.gov/docs-filing/elibrary...

  7. 76 FR 72699 - KC Hydro LLC of New Hampshire; Notice of Preliminary Permit Application Accepted for Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-25

    ....2001(a)(1)(iii) and the instructions on the Commission's Web site http:[sol][sol]www.ferc.gov/docs... registration, using the eComment system at http:[sol][sol]www.ferc.gov/docs-filing/ecomment.asp. You must... http:[sol][sol]www.ferc.gov/docs-filing/ elibrary.asp. Enter the docket number (P-14248-000) in the...

  8. The proteolytic system of pineapple stems revisited: Purification and characterization of multiple catalytically active forms.

    PubMed

    Matagne, André; Bolle, Laetitia; El Mahyaoui, Rachida; Baeyens-Volant, Danielle; Azarkan, Mohamed

    2017-06-01

    Crude pineapple proteases extract (aka stem bromelain; EC 3.4.22.4) is an important proteolytic mixture that contains enzymes belonging to the cysteine proteases of the papain family. Numerous studies have been reported aiming at the fractionation and characterization of the many molecular species present in the extract, but more efforts are still required to obtain sufficient quantities of the various purified protease forms for detailed physicochemical, enzymatic and structural characterization. In this work, we describe an efficient strategy towards the purification of at least eight enzymatic forms. Thus, following rapid fractionation on a SP-Sepharose FF column, two sub-populations with proteolytic activity were obtained: the unbound (termed acidic) and bound (termed basic) bromelain fractions. Following reversible modification with monomethoxypolyethylene glycol (mPEG), both fractions were further separated on Q-Sepharose FF and SP-Sepharose FF, respectively. This procedure yielded highly purified molecular species, all titrating ca. 1 mol of thiol group per mole of enzyme, with distinct biochemical properties. N-terminal sequencing allowed identifying at least eight forms with proteolytic activity. The basic fraction contained previously identified species, i.e. basic bromelain forms 1 and 2, ananain forms 1 and 2, and comosain (MEROPS identifier: C01.027). Furthermore, a new proteolytic species, showing similarities with basic bomelain forms 1 and 2, was discovered and termed bromelain form 3. The two remaining species were found in the acidic bromelain fraction and were arbitrarily named acidic bromelain forms 1 and 2. Both, acidic bromelain forms 1, 2 and basic bromelain forms 1, 2 and 3 are glycosylated, while ananain forms 1 and 2, and comosain are not. The eight protease forms display different amidase activities against the various substrates tested, namely small synthetic chromogenic compounds (DL-BAPNA and Boc-Ala-Ala-Gly-pNA), fluorogenic compounds

  9. 78 FR 6316 - Empire Pipeline, Inc. (Empire); Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC13-22-000] Empire Pipeline, Inc. (Empire); Notice of Filing Take notice that on November 29, 2012 Empire Pipeline Company (Empire) submitted a request for a waiver of the reporting requirement to file the FERC Form 2 CPA Certification for...

  10. 78 FR 2982 - Steuben Gas Storage Company (Steuben); Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC13-14-000] Steuben Gas Storage Company (Steuben); Notice of Filing Take notice that on October 19, 2012, Steuben Gas Storage Company (Steuben) submitted a request for a waiver of the reporting requirement to file the FERC Form 2-A...

  11. 75 FR 17406 - Hawthorn Oil Transportation; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-06

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC10-62-000] Hawthorn Oil Transportation; Notice of Filing March 31, 2010. Take notice that on February 12, 2010, Hawthorn Oil Transportation submitted a request for the waiver of the requirement to file the 2009 FERC Form No. 6 Annual...

  12. 78 FR 72673 - Zero Rate Reactive Power Rate Schedules; Notice of Staff Workshop

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-03

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AD14-1-000] Zero Rate Reactive Power Rate Schedules; Notice of Staff Workshop This notice establishes the location and date for... located at: https://www.ferc.gov/whats-new/registration/zero-rate-12-11-13-form.asp . The workshop will...

  13. Complete secretion of activable bovine prochymosin by genetically engineered L forms of Proteus mirabilis.

    PubMed Central

    Klessen, C; Schmidt, K H; Gumpert, J; Grosse, H H; Malke, H

    1989-01-01

    To circumvent problems encountered in the synthesis of active chymosin in a number of bacteria and fungi, a recombinant DNA L-form expression system that directed the complete secretion of fully activable prochymosin into the extracellular culture medium was developed. The expression plasmid constructions involved the in-frame fusion of prochymosin cDNA minus codons 1 to 4 to streptococcal pyrogenic exotoxin type A gene (speA') sequences, including the speA promoter, ribosomal binding site, and signal sequence and five codons of mature SpeA. Secretion of fusion prochymosin enzymatically and immunologically indistinguishable from bovine prochymosin was achieved after transformation of two stable protoplast type L-form strains derived from Proteus mirabilis. The secreted proenzyme was converted by autocatalytic processing to chymosin showing milk-clotting activity. In controlled laboratory fermentation processes, a maximum specific rate of activable prochymosin synthesis of 0.57 x 10(-3)/h was determined from the time courses of biomass dry weight and product formation. Yields as high as 40 +/- 10 micrograms/ml were obtained in the cell-free culture fluid of strain L99 carrying a naturally altered expression plasmid of increased segregational stability. The expression-secretion system described may be generally useful for production of recombinant mammalian proteins synthesized intracellularly as aberrantly folded insoluble aggregates. Images PMID:2499253

  14. Federal Energy Regulatory Commission financial statements, September 30, 1995 and 1994

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-02-12

    The attached report presents the results of the independent certified public accountant`s audit of the Federal Energy Regulatory Commission`s (FERC) financial statements as of September 30, 1995 and 1994. The auditors have expressed an unqualified opinion on the 1995 statements. Their reports on FERC`s internal control structure and on compliance with laws and regulations, and management letter are also provided.

  15. 76 FR 24561 - Reports, Forms and Recordkeeping Requirements; Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-02

    ... new information and environmental technologies that have substantially affected marine transportation..., including the use of automated collection techniques or other forms of information technology. A comment to...; Agency Information Collection Activity Under OMB Review AGENCY: Maritime Administration, DOT. ACTION...

  16. Inclusion bodies and purification of proteins in biologically active forms.

    PubMed

    Mukhopadhyay, A

    1997-01-01

    Even though recombinant DNA technology has made possible the production of valuable therapeutic proteins, its accumulation in the host cell as inclusion body poses serious problems in the recovery of functionally active proteins. In the last twenty years, alternative techniques have been evolved to purify biologically active proteins from inclusion bodies. Most of these remain only as inventions and very few are commercially exploited. This review summarizes the developments in isolation, refolding and purification of proteins from inclusion bodies that could be used for vaccine and non-vaccine applications. The second section involves a discussion on inclusion bodies, how they are formed, and their physicochemical properties. In vivo protein folding in Escherichia coli and kinetics of in vitro protein folding are the subjects of the third and fourth sections respectively. The next section covers the recovery of bioactive protein from inclusion bodies: it includes isolation of inclusion body from host cell debris, purification in denatured state alternate refolding techniques, and final purification of active molecules. Since purity and safety are two important issues in therapeutic grade proteins, the following three sections are devoted to immunological and biological characterization of biomolecules, nature, and type of impurities normally encountered, and their detection. Lastly, two case studies are discussed to demonstrate the sequence of process steps involved.

  17. Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.

    PubMed

    Liu, Xing; Zhang, Zhibin; Ruan, Jianbin; Pan, Youdong; Magupalli, Venkat Giri; Wu, Hao; Lieberman, Judy

    2016-07-07

    Inflammatory caspases (caspases 1, 4, 5 and 11) are activated in response to microbial infection and danger signals. When activated, they cleave mouse and human gasdermin D (GSDMD) after Asp276 and Asp275, respectively, to generate an N-terminal cleavage product (GSDMD-NT) that triggers inflammatory death (pyroptosis) and release of inflammatory cytokines such as interleukin-1β. Cleavage removes the C-terminal fragment (GSDMD-CT), which is thought to fold back on GSDMD-NT to inhibit its activation. However, how GSDMD-NT causes cell death is unknown. Here we show that GSDMD-NT oligomerizes in membranes to form pores that are visible by electron microscopy. GSDMD-NT binds to phosphatidylinositol phosphates and phosphatidylserine (restricted to the cell membrane inner leaflet) and cardiolipin (present in the inner and outer leaflets of bacterial membranes). Mutation of four evolutionarily conserved basic residues blocks GSDMD-NT oligomerization, membrane binding, pore formation and pyroptosis. Because of its lipid-binding preferences, GSDMD-NT kills from within the cell, but does not harm neighbouring mammalian cells when it is released during pyroptosis. GSDMD-NT also kills cell-free bacteria in vitro and may have a direct bactericidal effect within the cytosol of host cells, but the importance of direct bacterial killing in controlling in vivo infection remains to be determined.

  18. 77 FR 74647 - Agency Information Collection Activities: Proposed Collection, Comment Request: Form TO, Annual...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-17

    ...). Note that the swap definition excludes options on futures (which must be traded on a designated... COMMODITY FUTURES TRADING COMMISSION Agency Information Collection Activities: Proposed Collection, Comment Request: Form TO, Annual Notice Filing for Counterparties to Unreported Trade Options AGENCY...

  19. 78 FR 32261 - Agency Information Collection Activities: Immigrant Petition by Alien Entrepreneur, Form Number I...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-29

    ... DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services [OMB Control Number 1615-0026] Agency Information Collection Activities: Immigrant Petition by Alien Entrepreneur, Form Number I... collection. [[Page 32262

  20. 76 FR 48841 - Liberty Gas Storage, LLC; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-09

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC11-121-000] Liberty Gas Storage, LLC; Notice of Filing Take notice that on July 25, 2011, Liberty Gas Storage, LLC (Liberty) submitted a request for confirmation that it is not required to file FERC Form No. 2-A and will not be...

  1. 78 FR 8509 - PPL Colstrip I, LLC, PPL Colstrip II, LLC, PPL Montana, LLC; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... accounting and reporting requirements, except for sections 141.14 and 141.15. PPL Colstrip I requests such..., FERC Form No. 3-Q and meet other regulatory and accounting requirements imposed by these Parts from the... encourages electronic submission of protests and interventions in lieu of paper using the ``eFiling'' link at...

  2. 75 FR 9592 - National Grid USA; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-03

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC10-50-000; RM08-5-000] National Grid USA; Notice of Filing February 24, 2010. Take notice that on February 10, 2010, National Grid USA submitted a request for waiver of certain FERC Form No. 1 reporting requirements under Part 141 of...

  3. 76 FR 4103 - ETC Tiger Pipeline, LLC; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-24

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC11-25-000] ETC Tiger Pipeline, LLC; Notice of Filing January 13, 2011. Take notice that on December 30, 2010, ETC Tiger Pipeline, LLC submitted a request for a waiver of the reporting requirement to file the FERC Form 2 for 2010...

  4. Ceragenins are active against drug-resistant Candida auris clinical isolates in planktonic and biofilm forms.

    PubMed

    Hashemi, Marjan M; Rovig, John; Holden, Brett S; Taylor, Maddison F; Weber, Scott; Wilson, John; Hilton, Brian; Zaugg, Aaron L; Ellis, Samuel W; Yost, Connor D; Finnegan, Patrick M; Kistler, Charles K; Berkow, Elizabeth L; Deng, Shenglou; Lockhart, Shawn R; Peterson, Marnie; Savage, Paul B

    2018-06-01

    Candida auris has emerged as a serious threat to human health. Of particular concern are the resistance profiles of many clinical isolates, with some being resistant to multiple classes of antifungals. Measure susceptibilities of C. auris isolates, in planktonic and biofilm forms, to ceragenins (CSAs). Determine the effectiveness of selected ceragenins in gel and cream formulations in eradicating fungal infections in tissue explants. A collection of 100 C. auris isolates available at CDC was screened for susceptibility to a lead ceragenin. A smaller collection was used to characterize antifungal activities of other ceragenins against organisms in planktonic and biofilm forms. Effects of ceragenins on fungal cells and biofilms were observed via microscopy. An ex vivo model of mucosal fungal infection was used to evaluate formulated forms of lead ceragenins. Lead ceragenins displayed activities comparable to those of known antifungal agents against C. auris isolates with MICs of 0.5-8 mg/L and minimum fungicidal concentrations (MFCs) of 2-64 mg/L. No cross-resistance with other antifungals was observed. Fungal cell morphology was altered in response to ceragenin treatment. Ceragenins exhibited activity against sessile organisms in biofilms. Gel and cream formulations including 2% CSA-44 or CSA-131 resulted in reductions of over 4 logs against established fungal infections in ex vivo mucosal tissues. Ceragenins demonstrated activity against C. auris, suggesting that these compounds warrant further study to determine whether they can be used for topical applications to skin and mucosal tissues for treatment of infections with C. auris and other fungi.

  5. 75 FR 14438 - PPL Montana, LLC; Notice of Petition for Declaratory Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-25

    ..., 68 FR 49,846 (Aug. 19, 2003), FERC Stats. & Regs ] 31,146 (2003) (Order No. 2003); order on reh'g, Order No. 2003-A, 69 FR 15,932 (Mar. 26, 2004), FERC Stats. & Regs. 31,160, at P 531 (2004) (Order No. 2003-A); order on reh'g, Order No. 2003-B, 70 FR 265 (Jan. 4, 2005) (Order No. 2003-B) FERC Stats...

  6. 75 FR 24828 - Promoting a Competitive Market for Capacity Reassignments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-06

    ... software should be filed in native applications or print-to-PDF format and not in a scanned format. Mail... Group v. FERC, 225 F.3d 667 (DC Cir. 2000), aff'd sub nom. New York v. FERC, 535 U.S. 1 (2002). 3. With... (1993), aff'd in part and remanded in part sub nom. United Distribution Cos. v. FERC, 88 F.3d 1105 (DC...

  7. Validity and Reliability of International Physical Activity Questionnaire-Short Form in Chinese Youth

    ERIC Educational Resources Information Center

    Wang, Chao; Chen, Peijie; Zhuang, Jie

    2013-01-01

    Purpose: The psychometric profiles of the widely used International Physical Activity Questionnaire-Short Form (IPAQ-SF) in Chinese youth have not been reported. The purpose of this study was to examine the validity and reliability of the IPAQ-SF using a sample of Chinese youth. Method: One thousand and twenty-one youth (M[subscript age] = 14.26 ±…

  8. Cell Activation Mediated by Glycosylphosphatidylinositol-Anchored or Transmembrane Forms of CD14†

    PubMed Central

    Pugin, J.; Kravchenko, V. V.; Lee, J.-D.; Kline, L.; Ulevitch, R. J.; Tobias, P. S.

    1998-01-01

    CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein which functions as a receptor on myeloid cells for ligands derived from microbial pathogens such as lipopolysaccharide (LPS). We have studied the importance of the GPI tail of CD14 in signalling with the promonocytic cell line THP-1 expressing recombinant CD14 in a GPI-anchored form (THP1-wtCD14 cells) or in a transmembrane form (THP1-tmCD14). We found that, like other GPI-anchored molecules, GPI-anchored CD14 was recovered mainly from a Triton X-100-insoluble fraction, whereas transmembrane CD14 was fully soluble in Triton X-100. LPS induced cell activation of THP1-wtCD14 and of THP1-tmCD14 (protein tyrosine kinase phosphorylation, NF-κB activation, and cytokine production) in a very similar manner. However, anti-CD14 antibody-induced cross-linking caused a rapid calcium mobilization signal only in GPI-anchored CD14 cells. Studies with pharmacologic inhibitors of intracellular signalling events implicate phospholipase C and protein tyrosine kinases in the genesis of this antibody-induced calcium signal. Our results suggest that GPI anchoring and CD14 targeting to glycolipid-rich membrane microdomains are not required for LPS-mediated myeloid cell activation. GPI anchoring may however be important for other signalling functions, such as those events reflected by antibody cross-linking. PMID:9488411

  9. 76 FR 61709 - Agency Information Collection Activities; Proposed Collection; Comment Request; FDA Form 3728...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2011-N-0708] Agency Information Collection Activities; Proposed Collection; Comment Request; FDA Form 3728, Animal...: The Food and Drug Administration (FDA) is announcing an opportunity for public comment on the proposed...

  10. Pixantrone can be activated by formaldehyde to generate a potent DNA adduct forming agent

    PubMed Central

    Evison, Ben J.; Mansour, Oula C.; Menta, Ernesto; Phillips, Don R.; Cutts, Suzanne M.

    2007-01-01

    Mitoxantrone is an anti-cancer agent used in the treatment of breast and prostate cancers. It is classified as a topoisomerase II poison, however can also be activated by formaldehyde to generate drug–DNA adducts. Despite identification of this novel form of mitoxantrone–DNA interaction, excessively high, biologically irrelevant drug concentrations are necessary to generate adducts. A search for mitoxantrone analogues that could potentially undergo this reaction with DNA more efficiently identified Pixantrone as an ideal candidate. An in vitro crosslinking assay demonstrated that Pixantrone is efficiently activated by formaldehyde to generate covalent drug–DNA adducts capable of stabilizing double-stranded DNA in denaturing conditions. Pixantrone–DNA adduct formation is both concentration and time dependent and the reaction exhibits an absolute requirement for formaldehyde. In a direct comparison with mitoxantrone–DNA adduct formation, Pixantrone exhibited a 10- to 100-fold greater propensity to generate adducts at equimolar formaldehyde and drug concentrations. Pixantrone–DNA adducts are thermally and temporally labile, yet they exhibit a greater thermal midpoint temperature and an extended half-life at 37°C when compared to mitoxantrone–DNA adducts. Unlike mitoxantrone, this enhanced stability, coupled with a greater propensity to form covalent drug–DNA adducts, may endow formaldehyde-activated Pixantrone with the attributes required for Pixantrone–DNA adducts to be biologically active. PMID:17483512

  11. Lysosomal enzyme cathepsin B enhances the aggregate forming activity of exogenous α-synuclein fibrils.

    PubMed

    Tsujimura, Atsushi; Taguchi, Katsutoshi; Watanabe, Yoshihisa; Tatebe, Harutsugu; Tokuda, Takahiko; Mizuno, Toshiki; Tanaka, Masaki

    2015-01-01

    The formation of intracellular aggregates containing α-synuclein (α-Syn) is one of the key steps in the progression of Parkinson's disease and dementia with Lewy bodies. Recently, it was reported that pathological α-Syn fibrils can undergo cell-to-cell transmission and form Lewy body-like aggregates. However, little is known about how they form α-Syn aggregates from fibril seeds. Here, we developed an assay to study the process of aggregate formation using fluorescent protein-tagged α-Syn-expressing cells and examined the aggregate forming activity of exogenous α-Syn fibrils. α-Syn fibril-induced formation of intracellular aggregates was suppressed by a cathepsin B specific inhibitor, but not by a cathepsin D inhibitor. α-Syn fibrils pretreated with cathepsin B in vitro enhanced seeding activity in cells. Knockdown of cathepsin B also reduced fibril-induced aggregate formation. Moreover, using LAMP-1 immunocytochemistry and live-cell imaging, we observed that these aggregates initially occurred in the lysosome. They then rapidly grew larger and moved outside the boundary of the lysosome within one day. These results suggest that the lysosomal protease cathepsin B is involved in triggering intracellular aggregate formation by α-Syn fibrils. Copyright © 2015. Published by Elsevier Inc.

  12. Comparing Two Forms of Concept Map Critique Activities to Facilitate Knowledge Integration Processes in Evolution Education

    ERIC Educational Resources Information Center

    Schwendimann, Beat A.; Linn, Marcia C.

    2016-01-01

    Concept map activities often lack a subsequent revision step that facilitates knowledge integration. This study compares two collaborative critique activities using a Knowledge Integration Map (KIM), a form of concept map. Four classes of high school biology students (n?=?81) using an online inquiry-based learning unit on evolution were assigned…

  13. 76 FR 1149 - T.W. Phillips Pipeline Corp.; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-07

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC11-19-000] T.W. Phillips Pipeline Corp.; Notice of Filing December 30, 2010. Take notice that on December 17, 2010, T.W. Phillips Pipeline Corp. submitted a request for a waiver of the reporting requirement to file the FERC Form 2-A for...

  14. Inflammasome - activated gasdermin D causes pyroptosis by forming membrane pores

    PubMed Central

    Liu, Xing; Zhang, Zhibin; Ruan, Jianbin; Pan, Youdong; Magupalli, Venkat Giri; Wu, Hao; Lieberman, Judy

    2017-01-01

    Inflammatory caspases (caspases 1, 4, 5 and 11) are activated in response to microbial infection and danger signals. When activated, they cleave mouse and human gasdermin D (GSDMD) after Asp276 and Asp275, respectively, to generate an N-terminal cleavage product (GSDMD-NT) that triggers inflammatory death (pyroptosis) and release of inflammatory cytokines such as interleukin-1β1,2. Cleavage removes the C-terminal fragment (GSDMD-CT), which is thought to fold back on GSDMD-NT to inhibit its activation. However, how GSDMD-NT causes cell death is unknown. Here we show that GSDMD-NT oligomerizes in membranes to form pores that are visible by electron microscopy. GSDMD-NT binds to phosphatidylinositol phosphates and phosphatidylserine (restricted to the cell membrane inner leaflet) and cardiolipin (present in the inner and outer leaflets of bacterial membranes). Mutation of four evolutionarily conserved basic residues blocks GSDMD-NT oligomerization, membrane binding, pore formation and pyroptosis. Because of its lipid-binding preferences, GSDMD-NT kills from within the cell, but does not harm neighbouring mammalian cells when it is released during pyroptosis. GSDMD-NT also kills cell-free bacteria in vitro and may have a direct bactericidal effect within the cytosol of host cells, but the importance of direct bacterial killing in controlling in vivo infection remains to be determined. PMID:27383986

  15. [Active forms of oxygen and nitrogen in blood cells of patients with rheumatoid arthritis: effect of laser therapy].

    PubMed

    Ostrakhovich, E A; Ilich-Stoianovich, O; Afanas'ev, I B

    2001-01-01

    Infrared pulse laser therapy was studied for its impact on the production of active forms of oxygen and nitrogen by neutrophils from patients with rheumatoid arthritis (RA). The authors determined the non-activated and PMA-activated production of superoxide anion-radical, peroxynitrite, peripheral neurophilic NAD.PH-oxidase and superoxide dismutase activities, and the red blood cell concentrations of reduced glutathione. Before therapy, non-activation RA neurophilic production of superoxide was much higher than in donors. Laser therapy made this parameter normal. Similarly, neutrophilic peroxynitrite production (defined by dihydrorhodamine oxidation) in RA patients was 1.7 times higher than the normal values. IF-laser therapy decreased peroxynitrite production to the values observed in donors. It is important that the therapy caused increased SOD activity (that was lower in RA patients prior to therapy) up to apparently control values. Thus, IF-laser therapy has a certain antioxidative effect by increasing SOD activity in RA patients' blood cells and reducing the production of highly reactive oxygen and nitrogen forms.

  16. Role of C-terminal heptapeptide in pore-forming activity of antimicrobial agent, gaegurin 4.

    PubMed

    Kim, H J; Kim, S S; Lee, M H; Lee, B J; Ryu, P D

    2004-10-01

    Gaegurin 4 (GGN4) is an antimicrobial peptide of 37 amino acids isolated from the skin of a frog, Rana rugosa. GGN4 has a disulfide bond between the residues 31 and 37, which is highly conserved among the antimicrobial peptides isolated from skin of the genus, Rana. However, the role of this C-terminal heptapeptide motif is not well understood. In this work, we compared the membrane effects of the full-length GGN4 (C37) and GGN4 1-30 (C30), which is devoid of the C-terminal seven amino acids to elucidate the function of the C-terminal motif. C37 induced significantly larger membrane conductance (>10x) in the model lipid bilayers formed with acidic and neutral phospholipids and larger K+ efflux from gram-positive (>30x) and gram-negative bacteria. However, the pores induced by C37 and C30 were not different in their permeability to K+ over Cl- (permeability ratio of K+ to Cl- = 4.8-7.1). In addition, the pore-forming effect of C37 or C30 in acidic membranes was not different from that in neutral membranes. Furthermore, C37-induced K+ efflux was not significantly decreased by the reducing agent, dithiothreitol. The results indicate that C-terminal heptapeptide sequence plays an important role in maintaining the high pore-forming activity of GGN4, but does not participate in forming GGN4-induced pore structure. The disulfide bond in this region does not appear critical for such high ionophoric activity of GGN4.

  17. An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth

    PubMed Central

    Saha, Nayanendu; Eissman, Moritz F.; Xu, Kai; Llerena, Carmen; Kusebauch, Ulrike; Ding, Bi-Sen; Cao, Zhongwei; Rafii, Shahin; Ernst, Matthias; Scott, Andrew M.; Nikolov, Dimitar B.; Lackmann, Martin

    2016-01-01

    The transmembrane metalloprotease ADAM10 sheds a range of cell surface proteins, including ligands and receptors of the Notch, Eph, and erbB families, thereby activating signaling pathways critical for tumor initiation and maintenance. ADAM10 is thus a promising therapeutic target. Although widely expressed, its activity is normally tightly regulated. We now report prevalence of an active form of ADAM10 in tumors compared with normal tissues, in mouse models and humans, identified by our conformation-specific antibody mAb 8C7. Structure/function experiments indicate mAb 8C7 binds an active conformation dependent on disulfide isomerization and oxidative conditions, common in tumors. Moreover, this active ADAM10 form marks cancer stem-like cells with active Notch signaling, known to mediate chemoresistance. Importantly, specific targeting of active ADAM10 with 8C7 inhibits Notch activity and tumor growth in mouse models, particularly regrowth after chemotherapy. Our results indicate targeted inhibition of active ADAM10 as a potential therapy for ADAM10-dependent tumor development and drug resistance. PMID:27503072

  18. An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth.

    PubMed

    Atapattu, Lakmali; Saha, Nayanendu; Chheang, Chanly; Eissman, Moritz F; Xu, Kai; Vail, Mary E; Hii, Linda; Llerena, Carmen; Liu, Zhanqi; Horvay, Katja; Abud, Helen E; Kusebauch, Ulrike; Moritz, Robert L; Ding, Bi-Sen; Cao, Zhongwei; Rafii, Shahin; Ernst, Matthias; Scott, Andrew M; Nikolov, Dimitar B; Lackmann, Martin; Janes, Peter W

    2016-08-22

    The transmembrane metalloprotease ADAM10 sheds a range of cell surface proteins, including ligands and receptors of the Notch, Eph, and erbB families, thereby activating signaling pathways critical for tumor initiation and maintenance. ADAM10 is thus a promising therapeutic target. Although widely expressed, its activity is normally tightly regulated. We now report prevalence of an active form of ADAM10 in tumors compared with normal tissues, in mouse models and humans, identified by our conformation-specific antibody mAb 8C7. Structure/function experiments indicate mAb 8C7 binds an active conformation dependent on disulfide isomerization and oxidative conditions, common in tumors. Moreover, this active ADAM10 form marks cancer stem-like cells with active Notch signaling, known to mediate chemoresistance. Importantly, specific targeting of active ADAM10 with 8C7 inhibits Notch activity and tumor growth in mouse models, particularly regrowth after chemotherapy. Our results indicate targeted inhibition of active ADAM10 as a potential therapy for ADAM10-dependent tumor development and drug resistance. © 2016 Atapattu et al.

  19. A Natural Chimeric Pseudomonas Bacteriocin with Novel Pore-Forming Activity Parasitizes the Ferrichrome Transporter

    PubMed Central

    Kemland, Lieselore; Anoz-Carbonell, Ernesto; Buchanan, Susan K.; De Mot, René

    2017-01-01

    ABSTRACT Modular bacteriocins represent a major group of secreted protein toxins with a narrow spectrum of activity, involved in interference competition between Gram-negative bacteria. These antibacterial proteins include a domain for binding to the target cell and a toxin module at the carboxy terminus. Self-inhibition of producers is provided by coexpression of linked immunity genes that transiently inhibit the toxin’s activity through formation of bacteriocin-immunity complexes or by insertion in the inner membrane, depending on the type of toxin module. We demonstrate strain-specific inhibitory activity for PmnH, a Pseudomonas bacteriocin with an unprecedented dual-toxin architecture, hosting both a colicin M domain, potentially interfering with peptidoglycan synthesis, and a novel colicin N-type domain, a pore-forming module distinct from the colicin Ia-type domain in Pseudomonas aeruginosa pyocin S5. A downstream-linked gene product confers PmnH immunity upon susceptible strains. This protein, ImnH, has a transmembrane topology similar to that of Pseudomonas colicin M-like and pore-forming immunity proteins, although homology with either of these is essentially absent. The enhanced killing activity of PmnH under iron-limited growth conditions reflects parasitism of the ferrichrome-type transporter for entry into target cells, a strategy shown here to be used as well by monodomain colicin M-like bacteriocins from pseudomonads. The integration of a second type of toxin module in a bacteriocin gene could offer a competitive advantage against bacteria displaying immunity against only one of both toxic activities. PMID:28223456

  20. 78 FR 27965 - Agency Information Collection Activities: Submission for OMB Review; Comment Request Re Forms...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-13

    ... FEDERAL DEPOSIT INSURANCE CORPORATION Agency Information Collection Activities: Submission for OMB Review; Comment Request Re Forms Relating To Processing Deposit Insurance Claims AGENCY: Federal Deposit Insurance Corporation (FDIC). ACTION: Notice of proposed information collection renewal and comment request...

  1. Poliovirus 2C protein forms homo-oligomeric structures required for ATPase activity.

    PubMed

    Adams, Peter; Kandiah, Eaazhisai; Effantin, Grégory; Steven, Alasdair C; Ehrenfeld, Ellie

    2009-08-14

    The poliovirus protein 2C plays an essential role in viral RNA replication, although its precise biochemical activities or structural requirements have not been elucidated. The protein has several distinctive properties, including ATPase activity and membrane and RNA binding, that are conserved among orthologs of many positive-strand RNA viruses. Sequence alignments have placed these proteins in the SF3 helicase family, a subset of the AAA+ ATPase superfamily. A feature common to AAA+ proteins is the formation of oligomeric rings that are essential for their catalytic functions. Here we show that a recombinant protein, MBP-2C, in which maltose-binding protein was fused to 2C, formed soluble oligomers and that ATPase activity was restricted to oligomer-containing fractions from gel-filtration chromatography. The active fraction was visualized by negative-staining electron microscopy as ring-like particles composed of 5-8 protomers. This conclusion was confirmed by mass measurements obtained by scanning transmission electron microscopy. Mutation of amino acid residues in the 2C nucleotide-binding domain demonstrated that loss of the ability to bind or hydrolyze ATP did not affect oligomerization. Co-expression of active MBP-2C and inactive mutant proteins generated mixed oligomers that exhibited little ATPase activity, suggesting that incorporation of inactive subunits eliminates the function of the entire particle. Finally, deletion of the N-terminal 38 amino acids blocked oligomerization of the fusion protein and eliminated ATPase activity, despite retention of an unaltered nucleotide-binding domain.

  2. Poliovirus 2C Protein Forms Homo-oligomeric Structures Required for ATPase Activity*

    PubMed Central

    Adams, Peter; Kandiah, Eaazhisai; Effantin, Grégory; Steven, Alasdair C.; Ehrenfeld, Ellie

    2009-01-01

    The poliovirus protein 2C plays an essential role in viral RNA replication, although its precise biochemical activities or structural requirements have not been elucidated. The protein has several distinctive properties, including ATPase activity and membrane and RNA binding, that are conserved among orthologs of many positive-strand RNA viruses. Sequence alignments have placed these proteins in the SF3 helicase family, a subset of the AAA+ ATPase superfamily. A feature common to AAA+ proteins is the formation of oligomeric rings that are essential for their catalytic functions. Here we show that a recombinant protein, MBP-2C, in which maltose-binding protein was fused to 2C, formed soluble oligomers and that ATPase activity was restricted to oligomer-containing fractions from gel-filtration chromatography. The active fraction was visualized by negative-staining electron microscopy as ring-like particles composed of 5–8 protomers. This conclusion was confirmed by mass measurements obtained by scanning transmission electron microscopy. Mutation of amino acid residues in the 2C nucleotide-binding domain demonstrated that loss of the ability to bind or hydrolyze ATP did not affect oligomerization. Co-expression of active MBP-2C and inactive mutant proteins generated mixed oligomers that exhibited little ATPase activity, suggesting that incorporation of inactive subunits eliminates the function of the entire particle. Finally, deletion of the N-terminal 38 amino acids blocked oligomerization of the fusion protein and eliminated ATPase activity, despite retention of an unaltered nucleotide-binding domain. PMID:19520852

  3. CTEPP DATA COLLECTION FORM 09 (PERIODS 1-4 AND FOOD, FRUIT & VEG): CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data collection form is divided into two parts: Child Activity Diary and Food Survey. The Child Activity Diary collects information on the child's activities at home over the 48-hr monitoring period. The diary is divided into four time periods over the 48-hr monitoring inter...

  4. CTEPP DATA COLLECTION FORM 08 (PERIODS 1-5 AND FOOD, FRUIT & VEG): CHILD ACTIVITY DIARY AND FOOD SURVEY

    EPA Science Inventory

    This data collection form is divided into two parts: Child Activity Diary and Food Survey. The Child Activity Diary collects information on the child's activities at home over the 48-hr monitoring period. The diary is divided into five time periods over the 48-hr monitoring inter...

  5. Butyrylcholinesterase: K variant, plasma activity, molecular forms and rivastigmine treatment in Alzheimer's disease in a Southern Brazilian population.

    PubMed

    Bono, G F; Simão-Silva, D P; Batistela, M S; Josviak, N D; Dias, P F R; Nascimento, G A; Souza, R L R; Piovezan, M R; Souza, R K M; Furtado-Alle, L

    2015-02-01

    Alzheimer's disease (AD) is a neurodegenerative disorder in which there is a decline of cholinergic function. The symptomatic AD treatment involves the use of ChEIs (cholinesterase inhibitors) as rivastigimine, a dual inhibitor. The human butyrylcholinesterase (BChE) is an enzyme that has specific roles in cholinergic neurotransmission and it has been associated with AD. In the serum, BChE is found in four main molecular forms: G1 (monomer); G1-ALB (monomer linked to albumin); G2 (dimer); and G4 (tetramer). The interaction between the products of BCHE gene and CHE2 locus results in CHE2 C5+ and CHE2 C5- phenotypes. CHE2 C5+ phenotype and BChE-K are factors that influence on BChE activity. This work aimed to verify the proportions of BChE molecular forms, total and relative activity in 139 AD patients and 139 elderly controls, taking into account K variant, CHE2 locus, rivastigmine treatment and clinical dementia rating (CDR) of AD patients. Phenotypic frequencies of CHE2 C5+ and frequency of the carriers of the K allele were similar between groups. Total BChE activity in plasma was significantly lower in AD patients than in elderly controls. Furthermore, we found that reduction on plasma BChE activity is associated directly with AD progression in AD patients and that rivastigmine treatment has a stronger effect on BChE activity within the CDR2 group. The reduction in BChE activity did not occur proportionally in all molecular forms. Multiple regression analysis results confirmed that AD acts as the main factor in plasma BChE activity reduction and that severe stages are related with an even greater reduction. These findings suggest that the reduction of total plasma BChE and relative BChE molecular forms activity in AD patients is probably associated with a feedback mechanism and provides a future perspective of using this enzyme as a possible plasmatic secondary marker for AD. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. 18 CFR 357.3 - FERC Form No. 73, Oil Pipeline Data for Depreciation Analysis.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Pipeline Data for Depreciation Analysis. 357.3 Section 357.3 Conservation of Power and Water Resources... No. 73, Oil Pipeline Data for Depreciation Analysis. (a) Who must file. Any oil pipeline company.... 73, Oil Pipeline Data for Depreciation Analysis, available for review at the Commission's Public...

  7. Permission Forms

    ERIC Educational Resources Information Center

    Zirkel, Perry A.

    2005-01-01

    The prevailing practice in public schools is to routinely require permission or release forms for field trips and other activities that pose potential for liability. The legal status of such forms varies, but they are generally considered to be neither rock-solid protection nor legally valueless in terms of immunity. The following case and the…

  8. Galaxy Evolution in the Radio Band: The Role of Star-forming Galaxies and Active Galactic Nuclei

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mancuso, C.; Prandoni, I.; Lapi, A.

    We investigate the astrophysics of radio-emitting star-forming galaxies and active galactic nuclei (AGNs) and elucidate their statistical properties in the radio band, including luminosity functions, redshift distributions, and number counts at sub-mJy flux levels, which will be crucially probed by next-generation radio continuum surveys. Specifically, we exploit the model-independent approach by Mancuso et al. to compute the star formation rate functions, the AGN duty cycles, and the conditional probability of a star-forming galaxy to host an AGN with given bolometric luminosity. Coupling these ingredients with the radio emission properties associated with star formation and nuclear activity, we compute relevant statisticsmore » at different radio frequencies and disentangle the relative contribution of star-forming galaxies and AGNs in different radio luminosity, radio flux, and redshift ranges. Finally, we highlight that radio-emitting star-forming galaxies and AGNs are expected to host supermassive black holes accreting with different Eddington ratio distributions and to occupy different loci in the galaxy main-sequence diagrams. These specific predictions are consistent with current data sets but need to be tested with larger statistics via future radio data with multiband coverage on wide areas, as will become routinely achievable with the advent of the Square Kilometre Array and its precursors.« less

  9. 75 FR 16448 - Jordan Limited Partnership; Notice of Application Ready for Environmental Analysis and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-01

    ....O. Box 903, Gatlinburg, Tennessee 37738 (865) 436-0402. i. FERC Contact: Jeffrey Browning, (202) 502-8677 or jeffrey.browning@ferc.gov . j. Deadline for filing comments, recommendations, terms and...

  10. Forming a negative impression of another person correlates with activation in medial prefrontal cortex and amygdala.

    PubMed

    Iidaka, Tetsuya; Harada, Tokiko; Sadato, Norihiro

    2011-09-01

    Neural correlates involved in the formation of negative impression from face were investigated using event-related functional magnetic resonance imaging and a partial conditioning paradigm. Eighteen normal volunteers underwent imaging while they viewed the faces of two unfamiliar individuals: one individual's face was partially accompanied by negative emotion but the other's was not. After the volunteers learned the relationship between the faces and the emotion, they formed a more negative impression of the person's face when the emotion was presented. Subtraction analysis of the individuals' neutral faces revealed activation in the dorsal anterior cingulate cortex and superior temporal sulcus, but this activity did not correlate with the change of impression from face. On the other hand, the response in the left amygdala negatively correlated with the change of impression from face in the first run. Time modulation analysis revealed that activity in the dorsomedial prefrontal cortex associated with negative emotion was the largest in the initial part of the acquisition. These results suggest that a negative impression from face may be formed by orchestrated activity in the dorsomedial prefrontal cortex, dorsal anterior cingulate cortex and amygdala, and that the activity has a prominent role in the initial acquisition of negative emotion.

  11. 78 FR 52824 - Proposed Information Collection (Bowel and Bladder Care Billing Form) Activity: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-26

    ... and Bladder Care Billing Form) Activity: Comment Request AGENCY: Veterans Health Administration.... This notice solicits comments on the information needed to evaluate the Bowel and Bladder Care Billing... specifically to bowel and bladder care. DATES: Written comments and recommendations on the proposed collection...

  12. Hepatitis C virus NS3 helicase forms oligomeric structures that exhibit optimal DNA unwinding activity in vitro.

    PubMed

    Sikora, Bartek; Chen, Yingfeng; Lichti, Cheryl F; Harrison, Melody K; Jennings, Thomas A; Tang, Yong; Tackett, Alan J; Jordan, John B; Sakon, Joshua; Cameron, Craig E; Raney, Kevin D

    2008-04-25

    HCV NS3 helicase exhibits activity toward DNA and RNA substrates. The DNA helicase activity of NS3 has been proposed to be optimal when multiple NS3 molecules are bound to the same substrate molecule. NS3 catalyzes little or no measurable DNA unwinding under single cycle conditions in which the concentration of substrate exceeds the concentration of enzyme by 5-fold. However, when NS3 (100 nm) is equimolar with the substrate, a small burst amplitude of approximately 8 nm is observed. The burst amplitude increases as the enzyme concentration increases, consistent with the idea that multiple molecules are needed for optimal unwinding. Protein-protein interactions may facilitate optimal activity, so the oligomeric properties of the enzyme were investigated. Chemical cross-linking indicates that full-length NS3 forms higher order oligomers much more readily than the NS3 helicase domain. Dynamic light scattering indicates that full-length NS3 exists as an oligomer, whereas NS3 helicase domain exists in a monomeric form in solution. Size exclusion chromatography also indicates that full-length NS3 behaves as an oligomer in solution, whereas the NS3 helicase domain behaves as a monomer. When NS3 was passed through a small pore filter capable of removing protein aggregates, greater than 95% of the protein and the DNA unwinding activity was removed from solution. In contrast, only approximately 10% of NS3 helicase domain and approximately 20% of the associated DNA unwinding activity was removed from solution after passage through the small pore filter. The results indicate that the optimally active form of full-length NS3 is part of an oligomeric species in vitro.

  13. Functional Specificity of the Visual Word Form Area: General Activation for Words and Symbols but Specific Network Activation for Words

    ERIC Educational Resources Information Center

    Reinke, Karen; Fernandes, Myra; Schwindt, Graeme; O'Craven, Kathleen; Grady, Cheryl L.

    2008-01-01

    The functional specificity of the brain region known as the Visual Word Form Area (VWFA) was examined using fMRI. We explored whether this area serves a general role in processing symbolic stimuli, rather than being selective for the processing of words. Brain activity was measured during a visual 1-back task to English words, meaningful symbols…

  14. 50 CFR 223.301 - Special rules-marine and anadromous fishes.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... their behalf for the purpose of monitoring and evaluating the Opal Springs Hydroelectric Project (FERC... maintenance of the Opal Springs Hydroelectric Project (FERC Project No. 5891) as administered under a license...

  15. The pro-Forms of Insulin-Like Growth Factor I (IGF-I) Are Predominant in Skeletal Muscle and Alter IGF-I Receptor Activation

    PubMed Central

    Durzyńska, Julia; Philippou, Anastassios; Brisson, Becky K.; Nguyen-McCarty, Michelle

    2013-01-01

    IGF-I is a key regulator of muscle development and growth. The pre-pro-peptide produced by the Igf1gene undergoes several posttranslational processing steps to result in a secreted mature protein, which is thought to be the obligate ligand for the IGF-I receptor (IGF-IR). The goals of this study were to determine what forms of IGF-I exist in skeletal muscle, and whether the mature IGF-I protein was the only form able to activate the IGF-IR. We measured the proportion of IGF-I species in murine skeletal muscle and found that the predominant forms were nonglycosylated pro-IGF-I and glycosylated pro-IGF-I, which retained the C-terminal E peptide extension, instead of mature IGF-I. These forms were validated using samples subjected to viral expression of IGF-I combined with furin and glycosidase digestion. To determine whether the larger molecular weight IGF-I forms were also ligands for the IGF-IR, we generated each specific form through transient transfection of 3T3 cells and used the enriched media to perform kinase receptor activation assays. Compared with mature IGF-I, nonglycosylated pro-IGF-I had similar ability to activate the IGF-IR, whereas glycosylation of pro-IGF-I significantly reduced receptor activation. Thus, it is important to understand not only the quantity, but also the proportion of IGF-I forms produced, to evaluate the true biological activity of this growth factor. PMID:23407451

  16. Structure of the unliganded form of the proprotein convertase furin suggests activation by a substrate-induced mechanism

    PubMed Central

    Dahms, Sven O.; Arciniega, Marcelino; Steinmetzer, Torsten; Huber, Robert; Than, Manuel E.

    2016-01-01

    Proprotein convertases (PCs) are highly specific proteases required for the proteolytic modification of many secreted proteins. An unbalanced activity of these enzymes is connected to pathologies like cancer, atherosclerosis, hypercholesterolaemia, and infectious diseases. Novel protein crystallographic structures of the prototypical PC family member furin in different functional states were determined to 1.8–2.0 Å. These, together with biochemical data and modeling by molecular dynamics calculations, suggest essential elements underlying its unusually high substrate specificity. Furin shows a complex activation mechanism and exists in at least four defined states: (i) the “off state,” incompatible with substrate binding as seen in the unliganded enzyme; (ii) the active “on state” seen in inhibitor-bound furin; and the respective (iii) calcium-free and (iv) calcium-bound forms. The transition from the off to the on state is triggered by ligand binding at subsites S1 to S4 and appears to underlie the preferential recognition of the four-residue sequence motif of furin. The molecular dynamics simulations of the four structural states reflect the experimental observations in general and provide approximations of the respective stabilities. Ligation by calcium at the PC-specific binding site II influences the active-site geometry and determines the rotamer state of the oxyanion hole-forming Asn295, and thus adds a second level of the activity modulation of furin. The described crystal forms and the observations of different defined functional states may foster the development of new tools and strategies for pharmacological intervention targeting furin. PMID:27647913

  17. 77 FR 71432 - Agency Information Collection Activities: Application for Travel Document, Form Number I-131...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-30

    ...-0013] Agency Information Collection Activities: Application for Travel Document, Form Number I-131... information collection as DACA recipients that can establish a need to travel outside of the United States based on humanitarian, employment or education reasons will be able to request advance parole documents...

  18. Antimicrobial activity of root canal irrigants against biofilm forming pathogens- An in vitro study

    PubMed Central

    Ghivari, Sheetal Basavraj; Bhattacharya, Haimanti; Bhat, Kishore G.; Pujar, Madhu A.

    2017-01-01

    Aims: The aim of the study was to check the antimicrobial activity of the 5% Sodium hypochlorite, 2% Chlorhexidine, 0.10% Octenidine (OCT), and 2% Silver Zeolite (SZ) at different time intervals against a single species biofilm of Enterococcus faecalis, Staphylococcus aureus, and Candida albicans model prepared on a nitrocellulose membrane. Settings and Design: In vitro nitrocellulose biofilm model was used to check antibacterial efficacy of root canal irrigants. Materials and Methods: The in vitro nitrocellulose biofilm model was used to check the antibacterial activity of root canal irrigants. Single species biofilms were suspended into 96-well microtiter plate and treated with root canal irrigants for 1, 5, 10, 15, 30, and 60 s, respectively. The remaining microbial load in the form of colony-forming unit/ml after antimicrobial treatment was tabulated and data were statistically analyzed. Statistical Analysis: SPSS version 17, Kruskal–Wallis ANOVA, Mann–Whitney U-test, and Wilcoxon matched pair test (P < 0.05) were used. Results: All tested microorganisms were eliminated within 30 s by all the antimicrobial substances tested except normal saline. 2% chlorhexidine and 0.10% OCT were equally effective against C. albicans at 30 s. Conclusion: The newly tested irrigants have shown considerable antibacterial activity against selected single species biofilm. OCT (0.10%) can be used as an alternative endodontic irrigant. PMID:29279615

  19. 75 FR 38517 - Environmental Impact Statements; Notice of Availability

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-02

    ..., FERC, CA, Kilarc-Cow Creek Hydroelectric Project (FERC Project No. 606) Proposes to Surrender the License for Operation Project, Old Crow Creek and South Cow Creek, Shasta County, CA, Comment Period Ends...

  20. 78 FR 73237 - Reports, Forms and Record Keeping Requirements Agency Information Collection Activity Under OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-05

    ... of information technology. A comment to OMB is most effective if OMB receives it within 30 days of...-0088] Reports, Forms and Record Keeping Requirements Agency Information Collection Activity Under OMB... Information Collection Request (ICR) abstracted below has been forwarded to the Office of Management and...

  1. 78 FR 26648 - Agency Information Collection Activities: Passenger List/Crew List (CBP Form I-418)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-07

    ... DEPARTMENT OF HOMELAND SECURITY U.S. Customs and Border Protection Agency Information Collection Activities: Passenger List/Crew List (CBP Form I-418) AGENCY: U.S. Customs and Border Protection (CBP... prescribed by the Department of Homeland Security, Customs and Border Protection (CBP), for use by masters...

  2. 75 FR 4057 - Jordan Limited Partnership; Notice of Application Accepted for Filing and Soliciting Motions To...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-26

    ....O. Box 903, Gatlinburg, Tennessee 37738 (865) 436-0402. i. FERC Contact: Jeffrey Browning, (202) 502-8677 or jeffrey.browning@ferc.gov . j. Deadline for filing motions to intervene and protests: 60 days...

  3. 78 FR 69658 - Domtar Wisconsin Dam Corporation; Notice of Application To Amend License and Accepted for Filing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-20

    ... Point Basse Avenue, Nekoosa, Wisconsin 54457; telephone (715) 886-7711. i. FERC Contact: Linda Stewart at (202) 502-6680; or email at linda.stewart@ferc.gov . j. Deadline for filing comments, motions to...

  4. Two forms of alpha-amylase in mantle tissue of Mytilus galloprovincialis: purification and molecular properties of form II.

    PubMed

    Lombraña, M; Suárez, P; San Juan, F

    2005-09-01

    alpha-Amylase activity has been shown for the first time in a non-digestive tissue from Mytilus galloprovincialis. alpha-amylase from mussel mantle tissue has been purified by affinity chromatography on insoluble starch, followed by gel-filtration chromatography on Superdex-200. The chromatographic and electrophoretic behaviour of M. galloprovincialis alpha-amylase and stability characteristics suggest two forms of this enzyme: one form forming stable aggregates (form I) and a monomeric form (form II) that is more abundant, active and unstable. Both forms show an inverse quantitative variation. Purified form II was highly unstable and the molecular mass was estimated to be 66 kDa by sodium dodecyl sulphate (SDS)-gel electrophoresis. Maximum activity was noted at pH 6.5 and 35 degrees C.

  5. Disulfide bonding arrangements in active forms of the somatomedin B domain of human vitronectin.

    PubMed

    Kamikubo, Yuichi; De Guzman, Roberto; Kroon, Gerard; Curriden, Scott; Neels, Jaap G; Churchill, Michael J; Dawson, Philip; Ołdziej, Stanisław; Jagielska, Anna; Scheraga, Harold A; Loskutoff, David J; Dyson, H Jane

    2004-06-01

    The N-terminal cysteine-rich somatomedin B (SMB) domain (residues 1-44) of the human glycoprotein vitronectin contains the high-affinity binding sites for plasminogen activator inhibitor-1 (PAI-1) and the urokinase receptor (uPAR). We previously showed that the eight cysteine residues of recombinant SMB (rSMB) are organized into four disulfide bonds in a linear uncrossed pattern (Cys(5)-Cys(9), Cys(19)-Cys(21), Cys(25)-Cys(31), and Cys(32)-Cys(39)). In the present study, we use an alternative method to show that this disulfide bond arrangement remains a major preferred one in solution, and we determine the solution structure of the domain using NMR analysis. The solution structure shows that the four disulfide bonds are tightly packed in the center of the domain, replacing the traditional hydrophobic core expected for a globular protein. The few noncysteine hydrophobic side chains form a cluster on the outside of the domain, providing a distinctive binding surface for the physiological partners PAI-1 and uPAR. The hydrophobic surface consists mainly of side chains from the loop formed by the Cys(25)-Cys(31) disulfide bond, and is surrounded by conserved acidic and basic side chains, which are likely to contribute to the specificity of the intermolecular interactions of this domain. Interestingly, the overall fold of the molecule is compatible with several arrangements of the disulfide bonds. A number of different disulfide bond arrangements were able to satisfy the NMR restraints, and an extensive series of conformational energy calculations performed in explicit solvent confirmed that several disulfide bond arrangements have comparable stabilization energies. An experimental demonstration of the presence of alternative disulfide conformations in active rSMB is provided by the behavior of a mutant in which Asn(14) is replaced by Met. This mutant has the same PAI-1 binding activity as rVN1-51, but its fragmentation pattern following cyanogen bromide treatment is

  6. Five of 12 forms of vaccinia virus-expressed human hepatic cytochrome P450 metabolically activate aflatoxin B sub 1

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Aoyama, Toshifumi; Yamano, Shigeru; Gelboin, H.V.

    Twelve forms of human hepatic cytochrome P450 were expressed in hepatoma cells by means of recombinant vaccinia viruses. The expressed P450s were analyzed for their abilities to activate the potent hepatocarcinogen aflatoxin B{sub 1} to metabolites having mutagenic or DNA-binding properties. Five forms, P450s IA2, IIA3, IIB7, IIIA3, and IIIA4, activated aflatoxin B{sub 1} to mutagenic metabolites as assessed by the production of His revertants of Salmonella typhimurium in the Ames test. The same P450s catalyzed conversion of aflatoxin B{sub 1} to DNA-bound derivatives as judged by an in situ assay in which the radiolabeled carcinogen was incubated with cellsmore » expressing the individual P450 forms. Seven other human P450s, IIC8, IIC9, IID6, IIE1, IIF1, and IIIA5, and IVB1, did not significantly activate aflatoxin B{sub 1} as measured by both the Ames test and the DNA-binding assay. Moreover, polyclonal anti-rat liver P450 antibodies that crossreact with individual human P450s IA2, IIA3, IIIA3, and IIIA4 each inhibited aflatoxin B{sub 1} activation catalyzed by human liver S-9 extracts. Inhibition ranged from as low as 10% with antibody against IIA3 to as high as 65% with antibody against IIIA3 and IIIA4. These results establish that metabolic activation of aflatoxin B{sub 1} in human liver involves the contribution of multiple forms of P450.« less

  7. 75 FR 27771 - Mississippi 13 Hydro, LLC; Notice of Preliminary Permit Application Accepted for Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ..., Idaho 83442; phone: (208) 745-0834. FERC Contact: Bryan Roden-Reynolds at (202) 502-6618, or via e-mail at bryan.roden-reynolds@ferc.gov . The deadline for filing comments, motions to intervene, competing...

  8. 75 FR 27770 - Mississippi 12 Hydro, LLC; Notice of Preliminary Permit Application Accepted for Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ..., Rigby, Idaho 83442; phone: (208) 745-0834. FERC Contact: Bryan Roden-Reynolds at (202) 502-6618, or via e-mail at bryan.roden-reynolds@ferc.gov . The deadline for filing comments, motions to intervene...

  9. 75 FR 27770 - Mississippi 17 Hydro, LLC; Notice of Preliminary Permit Application Accepted for Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ..., Idaho 83442; phone: (208) 745-0834. FERC Contact: Bryan Roden-Reynolds at (202) 502-6618, or via e-mail at bryan.roden-reynolds@ferc.gov . The deadline for filing comments, motions to intervene, competing...

  10. 75 FR 27769 - Mississippi 16 Hydro, LLC; Notice of Preliminary Permit Application Accepted for Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-18

    ..., Idaho 83442; phone: (208) 745-0834. FERC Contact: Bryan Roden-Reynolds at (202) 502-6618, or via e-mail at bryan.roden-reynolds@ferc.gov . The deadline for filing comments, motions to intervene, competing...

  11. 76 FR 57724 - Notice of Application Tendered for Filing With the Commission, Accepted for Filing With the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-16

    ..., Westminster, MA 01473, (978) 874-1010, [email protected] . i. FERC Contact: Jeff Browning, (202) 502-8677, or e-mail at Jeffrey.Browning@ferc.gov . j. Deadline for Filing Motions to Intervene And Protests...

  12. 76 FR 60016 - Bellwood Hydro, LLC; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-28

    ...-high rock or earth fill main dam and a new 2,500-foot-long, 60-foot-high rock or earth fill saddle dam...-long, 185-foot-high rock or earth fill dam forming a lower reservoir having a surface area of 120 acres... registration, using the eComment system at http://www.ferc.gov/docs-filing/ecomment.asp . You must include your...

  13. Effects of pharmaceutical processing on pepsin activity during the formulation of solid dosage forms.

    PubMed

    Kristó, Katalin; Pintye-Hódi, Klára

    2013-02-01

    The main aim of this study was to investigate the effects of pharmaceutical technological methods on pepsin activity during the formulation of solid dosage forms. The circumstances of direct compression and wet granulation were modeled. During direct compression, the heat and the compression force must be taken into consideration. The effects of these parameters were investigated in three materials (pure pepsin, and 1:1 (w/w) pepsin-tartaric acid and 1:1 (w/w) pepsin-citric acid powder mixtures). It was concluded that direct compression is appropriate for the formulation of solid dosage forms containing pepsin through application without acids or with acids at low compression force. The effects of wet granulation were investigated with a factorial design for the same three materials. The factors were time, temperature and moisture content. There was no significant effect of the factors when acids were not applied. Temperature was a significant factor when acids were applied. The negative effect was significantly higher for citric acid than for tartaric acid. It was found that wet granulation can be utilized for the processing of pepsin into solid dosage forms under well-controlled circumstances. The application of citric acid is not recommended during the formulation of solid dosage forms through wet granulation. A mathematically based optimization may be necessary for preformulation studies of the preparation of dosage forms containing sensitive enzymes.

  14. 77 FR 65703 - Agency Information Collection Activities: Application for Family Unity Benefits, Form I-817...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... DEPARTMENT OF HOMELAND SECURITY U.S. Citizenship and Immigration Services [OMB Control Number 1615-0005] Agency Information Collection Activities: Application for Family Unity Benefits, Form I-817, Revision of a Currently Approved Collection ACTION: 60-Day Notice. The Department of Homeland Security (DHS), U.S. Citizenship and Immigration...

  15. 78 FR 13886 - Agency Information Collection Activities: Comment Request for the Production Estimate (2 Forms)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ... DEPARTMENT OF THE INTERIOR U.S. Geological Survey [USGS-GX13LR000F60100] Agency Information Collection Activities: Comment Request for the Production Estimate (2 Forms) AGENCY: U.S. Geological Survey... Officer, U.S. Geological Survey, 12201 Sunrise Valley Drive, Mail Stop 807, Reston, VA 20192 (mail); 703...

  16. Disruption of the Crithidia fasciculata RNH1 gene results in the loss of two active forms of ribonuclease H.

    PubMed Central

    Ray, D S; Hines, J C

    1995-01-01

    Both prokaryotic and eukaryotic cells contain multiple forms of ribonuclease H, a ribonuclease that specifically degrades the RNA strand of RNA-DNA hybrids and which has been implicated in the processing of initiator RNAs and in the removal of RNA primers from Okazaki fragments. The Crithidia fasciculata RNH1 gene encodes an RNase H and was shown to be a single-copy gene in this diploid trypanosomatid. The RNH1 gene has been disrupted by targeted gene disruption using hygromycin or G418 drug-resistance cassettes. Major active forms of RNase H (38 and 45 kDa) were observed on activity gels of extracts of wild-type cells or cells in which one allele of RNH1 was disrupted. Both the 38 and 45 kDa activities were absent in extracts of cells in which both alleles of RNH1 were disrupted indicating that both forms of the C.fasciculata RNase H are encoded by the RNH1 gene. Images PMID:7630731

  17. The mineralogy of newly formed dust in active galactic nuclei

    NASA Astrophysics Data System (ADS)

    Srinivasan, Sundar; Kemper, F.; Zhou, Yeyan; Hao, Lei; Gallagher, Sarah C.; Shangguan, Jinyi; Ho, Luis C.; Xie, Yanxia; Scicluna, Peter; Foucaud, Sebastien; Peng, Rita H. T.

    2017-12-01

    The tori around active galactic nuclei (AGN) are potential formation sites for large amounts of dust, and they may help resolve the so-called dust budget crisis at high redshift. We investigate the dust composition in 53 of the 87 Palomar Green (PG) quasars showing the 9.7 μm silicate feature in emission. By simultaneously fitting the mid-infrared spectroscopic features and the underlying continuum, we estimate the mass fraction in various amorphous and crystalline dust species. We find that the dust consists predominantly of alumina and amorphous silicates, with a small fraction in crystalline form. The mean crystallinity is 8 ±6%, with more than half of the crystallinities greater than 5%, well above the upper limit determined for the Galaxy. Higher values of crystallinity are found for higher oxide fractions and for more luminous sources.

  18. Fall prevention with supplemental and active forms of vitamin D: a meta-analysis of randomised controlled trials.

    PubMed

    Bischoff-Ferrari, H A; Dawson-Hughes, B; Staehelin, H B; Orav, J E; Stuck, A E; Theiler, R; Wong, J B; Egli, A; Kiel, D P; Henschkowski, J

    2009-10-01

    To test the efficacy of supplemental vitamin D and active forms of vitamin D with or without calcium in preventing falls among older individuals. We searched Medline, the Cochrane central register of controlled trials, BIOSIS, and Embase up to August 2008 for relevant articles. Further studies were identified by consulting clinical experts, bibliographies, and abstracts. We contacted authors for additional data when necessary. Review methods Only double blind randomised controlled trials of older individuals (mean age 65 years or older) receiving a defined oral dose of supplemental vitamin D (vitamin D(3) (cholecalciferol) or vitamin D(2) (ergocalciferol)) or an active form of vitamin D (1alpha-hydroxyvitamin D(3) (1alpha-hydroxycalciferol) or 1,25-dihydroxyvitamin D(3) (1,25-dihydroxycholecalciferol)) and with sufficiently specified fall assessment were considered for inclusion. Eight randomised controlled trials (n=2426) of supplemental vitamin D met our inclusion criteria. Heterogeneity among trials was observed for dose of vitamin D (700-1000 IU/day v 200-600 IU/day; P=0.02) and achieved 25-hydroxyvitamin D(3) concentration (25(OH)D concentration: <60 nmol/l v >or=60 nmol/l; P=0.005). High dose supplemental vitamin D reduced fall risk by 19% (pooled relative risk (RR) 0.81, 95% CI 0.71 to 0.92; n=1921 from seven trials), whereas achieved serum 25(OH)D concentrations of 60 nmol/l or more resulted in a 23% fall reduction (pooled RR 0.77, 95% CI 0.65 to 0.90). Falls were not notably reduced by low dose supplemental vitamin D (pooled RR 1.10, 95% CI 0.89 to 1.35; n=505 from two trials) or by achieved serum 25-hydroxyvitamin D concentrations of less than 60 nmol/l (pooled RR 1.35, 95% CI 0.98 to 1.84). Two randomised controlled trials (n=624) of active forms of vitamin D met our inclusion criteria. Active forms of vitamin D reduced fall risk by 22% (pooled RR 0.78, 95% CI 0.64 to 0.94). Supplemental vitamin D in a dose of 700-1000 IU a day reduced the risk of

  19. DFT study of quercetin activated forms involved in antiradical, antioxidant, and prooxidant biological processes.

    PubMed

    Fiorucci, Sébastien; Golebiowski, Jérôme; Cabrol-Bass, Daniel; Antonczak, Serge

    2007-02-07

    Quercetin, one of the most representative flavonoid compounds, is involved in antiradical, antioxidant, and prooxidant biological processes. Despite a constant increase of knowledge on both positive and negative activities of quercetin, it is unclear which activated form (quinone, semiquinone, or deprotonated) actually plays a role in each of these processes. Structural, electronic, and energetic characteristics of quercetin, as well as the influence of a copper ion on all of these parameters, are studied by means of quantum chemical electronic structure calculations. Introduction of thermodynamic cycles together with the role of coreactive compounds, such as reactive oxygen species, gives a glimpse of the most probable reaction schemes. Such a theoretical approach provides another hint to clarify which reaction is likely to occur within the broad range of quercetin biological activities.

  20. 75 FR 48965 - Ameren Services Company; Notice of Petition for Declaratory Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-12

    ..., Promoting Transmission Investment Through Pricing Reform, Order No. 679, 2006-2007 FERC Stats. & Regs., Regs. Preambles ] 31,222, order on reh'g, Order No. 679-A, 2006-2007 FERC Stats. & Regs., Regs. Preambles ] 31,236...

  1. 76 FR 1153 - Atlantic Grid Operations A LLC, Atlantic Grid Operations B LLC, Atlantic Grid Operations C LLC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-07

    ...\\ Promoting Transmission Investment through Pricing Reform, Order No. 679, 2006-2007 FERC Stats. & Regs., Regs. Preambles ] 31,222, order on reh'g, Order No. 679-A, 2006-2007 FERC Stats. & Regs., Regs. Preambles ] 31,236...

  2. Ex Vivo and In Vivo Biological Effects of a Truncated Form of the Receptor Tyrosine Kinase Stk When Activated by Interaction with the Friend Spleen Focus-Forming Virus Envelope Glycoprotein or by Point Mutation

    PubMed Central

    Rulli, Karen; Yugawa, Takashi; Hanson, Charlotte; Thompson, Delores; Ruscetti, Sandra; Nishigaki, Kazuo

    2004-01-01

    The erythroleukemia-inducing Friend spleen focus-forming virus (SFFV) encodes a unique envelope protein, gp55, which interacts with the erythropoietin (Epo) receptor complex, causing proliferation and differentiation of erythroid cells in the absence of Epo. Susceptibility to SFFV-induced erythroleukemia is conferred by the Fv-2 gene, which encodes a short form of the receptor tyrosine kinase Stk/Ron (sf-Stk) only in susceptible strains of mice. We recently demonstrated that sf-Stk becomes activated by forming a strong interaction with SFFV gp55. To examine the biological consequences of activated sf-Stk on erythroid cell growth, we prepared retroviral vectors which express sf-Stk, either in conjunction with gp55 or alone in a constitutively activated mutant form, and tested them for their ability to induce Epo-independent erythroid colonies ex vivo and disease in mice. Our data indicate that both gp55-activated sf-Stk and the constitutively activated mutant of sf-Stk induce erythroid cells from Fv-2-susceptible and Fv-2-resistant (sf-Stk null) mice to form Epo-independent colonies. Mutational analysis of sf-Stk indicated that a functional kinase domain and 8 of its 12 tyrosine residues are required for the induction of Epo-independent colonies. Further studies demonstrated that coexpression of SFFV gp55 with sf-Stk significantly extends the half-life of the kinase. When injected into Fv-2-resistant mice, neither the gp55-activated sf-Stk nor the constitutively activated mutant caused erythroleukemia. Surprisingly, both Fv-2-susceptible and -resistant mice injected with the gp55-sf-Stk vector developed clinical signs not previously associated with SFFV-induced disease. We conclude that sf-Stk, activated by either point mutation or interaction with SFFV gp55, is sufficient to induce Epo-independent erythroid colonies from both Fv-2-susceptible and -resistant mice but is unable to cause erythroleukemia in Fv-2-resistant mice. PMID:15078939

  3. Structure and Chemical Synthesis of a Biologically Active Form of Renilla (Sea Pansy) Luciferin*

    PubMed Central

    Hori, Kazuo; Cormier, Milton J.

    1973-01-01

    The structure of a biologically active form of Renilla (sea pansy) luciferin has been elucidated; this structure, confirmed by total chemical synthesis, is 3,7-dihydro-2-methyl-6-(p-hydroxyphenyl)-8-benzylimidazo [1,2-a] pyrazin-3-one. In the natural compound the methyl group at the 2 position is replaced by an unknown, more complex group. For this reason the synthetic compound is 10% as active as the natural compound in producing light with Renilla luciferase. However, the spectral properties of the two compounds are identical. In addition the rates of the luminescent reaction with both compounds are similar, and the color of the light produced is identical in each case. A compound isolated from the calcium-triggered photoprotein aequorin has been identified by Shimomura and Johnson [(1972) Biochemistry 11, 1602] to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine. This compound forms an integral part of the structure of Renilla luciferin. This, and other evidence, suggests that the structure elucidated for Renilla luciferin is a more general one associated with the luciferins of most, if not all, bioluminescent coelenterates. PMID:16592045

  4. Channel-forming activity in the venom of the cockroach-hunting wasp, Ampulex compressa.

    PubMed

    Gincel, Dan; Haspel, Gal; Libersat, Frederic

    2004-05-01

    The parasitoid solitary wasp Ampulex compressa uses the cockroach Periplaneta americana as a food supply for its larvae. To subdue its prey, the wasp injects a venom cocktail into the brain of the cockroach. We investigated channel activity of A. compressa venom by collecting venom and incorporating it into a planar lipid bilayer. The venom, reconstituted into the bilayer, showed ion channel activity, forming a fast-fluctuating channel with a small conductance of 20+/-0.1pS, with no voltage sensitivity. These channels were not observed when the venom was digested with proteases before application to the bilayer, but were not affected by exposure to protease after their incorporation into the bilayer, indicating that the active venom component is a peptide. The channels were found to be cation selective with similar selectivity for the monovalent cations K(+), Li(+) and Na(+), but showed high selectivity against anions (Cl(-)) and divalent cations (Ca(2+) and Mg(2+)). This study is the first demonstration and biophysical characterization of channel activity in the venom of A. compressa. The possible functional significance of this channel activity is discussed in light of the unusual nature of the effects of this wasp venom on the behavior of its prey.

  5. Peroxidase-mediated dealkylation of tamoxifen, detected by electrospray ionization-mass spectrometry, and activation to form DNA adducts.

    PubMed

    Gaikwad, Nilesh W; Bodell, William J

    2012-01-15

    Tamoxifen (TAM) is extensively used for the treatment and prevention of breast cancer. Associated with TAM treatment is a two- to eightfold increase in risk of endometrial cancer. To understand the mechanisms associated with this increased risk several pathways for TAM metabolism and DNA adduct formation have been studied. The purpose of this study was to investigate the role of peroxidase enzymes in the metabolism of TAM and its activation to form DNA adducts. Using advanced tandem mass spectrometry we have investigated the peroxidase-mediated metabolism of TAM. Incubation of TAM with horseradish peroxidase (HRP) and H(2)O(2) produced multiple metabolites. Electrospray ionization-MS/MS analysis of the metabolites demonstrated a peak at 301.3m/z with daughter ions at 183.0, 166.9, 128.9, and 120.9m/z, which identified the metabolite as metabolite E (ME). The levels of ME were significantly inhibited by the addition of ascorbic acid to the incubation mixture. Co-incubation of either TAM or ME and DNA with HRP and H(2)O(2) produced three DNA adducts with a RAL of 1.97±0.01×10(-7) and 8.45±2.7×10(-7). Oxidation of ME with MnO(2) produced metabolite E quinone methide (MEQM). Furthermore, incubation of either TAM or ME with HRP and H(2)O(2) resulted in formation of MEQM. Reaction of calf thymus DNA with MEQM produced three DNA adducts with a RAL of 9.8±1.0×10(-7). Rechromatography analyses indicated that DNA adducts 1, 2, and 3 formed in the HRP activation of either TAM or ME were the same as those formed by the chemical reaction of DNA with MEQM. The results of these studies demonstrate that peroxidase enzymes can both metabolize TAM to form the primary metabolite ME and activate ME to a quinone methide intermediate, which reacts with DNA to form adducts. It is possible that peroxidase enzymes or peroxidase-like activity in endometrium could contribute to the formation of DNA damage and genotoxic effects in endometrium after TAM administration. Published by

  6. 76 FR 44902 - FFP Project 90, LLC; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-27

    ... Contact: Bryan Roden-Reynolds at (202) 502-6618, or via e-mail at bryan.roden-reynolds@ferc.gov . Deadline... http://www.ferc.gov/docs-filing/elibrary.asp . Enter the docket number (P-14218) in the docket number...

  7. 78 FR 75559 - Kaukauna Utilities; Notice of Intent To File License Application, Filing of Pre-Application...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-12

    ... Operations, Kaukauna Utilities, 777 Island Street, Kaukauna, WI 54130; phone: (920) 462-0220; email: wi.org ">[email protected] wi.org . i. FERC Contact: Isis Johnson at (202) 502-6346; or email at isis.johnson@ferc.gov...

  8. 78 FR 79427 - Greenwood County; Notice of Application Accepted for Filing and Soliciting Comments, Motions To...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-30

    ... Commission's Web site at http://www.ferc.gov/docs-filing/efiling.asp . Commenters can submit brief comments... calling (202) 502-8371. This filing may also be viewed on the Commission's Web site at http://www.ferc.gov...

  9. 77 FR 28595 - Vlast LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for Blanket...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-15

    ... the FERC Online links at http://www.ferc.gov . To facilitate electronic service, persons with Internet access who will eFile a document and/or be listed as a contact for an intervenor must create and validate...

  10. Single-exponential activation behavior behind the super-Arrhenius relaxations in glass-forming liquids.

    PubMed

    Wang, Lianwen; Li, Jiangong; Fecht, Hans-Jörg

    2010-11-17

    The reported relaxation time for several typical glass-forming liquids was analyzed by using a kinetic model for liquids which invoked a new kind of atomic cooperativity--thermodynamic cooperativity. The broadly studied 'cooperative length' was recognized as the kinetic cooperativity. Both cooperativities were conveniently quantified from the measured relaxation data. A single-exponential activation behavior was uncovered behind the super-Arrhenius relaxations for the liquids investigated. Hence the mesostructure of these liquids and the atomic mechanism of the glass transition became clearer.

  11. 30 CFR 285.637 - When may I commence commercial operations on my commercial lease?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... commercial lease? 285.637 Section 285.637 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, REGULATION, AND ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE RENEWABLE ENERGY ALTERNATE USES OF EXISTING... activities on your lease that: (a) Do not require a FERC license (i.e., wind), then you may commence...

  12. Nitric oxide (NO), the only nitrogen monoxide redox form capable of activating soluble guanylyl cyclase.

    PubMed

    Dierks, E A; Burstyn, J N

    1996-06-28

    In the present study, we determined that of the redox forms of nitrogen monoxide, NO-, NO and NO+, only NO significantly activates soluble guanylyl cyclase (GTP pyrophosphate-lyase cyclizing, EC 4.6.1.2). Neither of the NO-donors tested, Angeli's salt (Na2N2O3) or Piloty's acid (C6H5SO2NHOH), caused a change in the guanylyl cyclase activity relative to the basal activity level. Interference by other reaction products was eliminated as a possible explanation for the lack of activation. To the extent that NO+ could be stabilized in aqueous solution, by dissolution of the nitrosonium salt NOPF6 in dry organic solvent prior to addition to the enzyme in buffer, NO+ had no effect on the activity of soluble guanylyl cyclase. The counter-ion, PF6-, had a minimal effect on the enzyme activity and, therefore was, not responsible for the lack of activation by NO+. These observations suggest that NO- is the natural activator of soluble guanylyl cyclase and is reasonably identical with endothelium-derived relaxing factor, the physiological regulator of soluble guanylyl cyclase activity.

  13. The Active Form of the Saccharomyces cerevisiae Ribonucleotide Reductase Small Subunit Is a Heterodimer in Vitro and in Vivo†

    PubMed Central

    Perlstein, Deborah L.; Ge, Jie; Ortigosa, Allison D.; Robblee, John H.; Zhang, Zhen; Huang, Mingxia; Stubbe, JoAnne

    2015-01-01

    The class I ribonucleotide reductases (RNRs) are composed of two homodimeric subunits: R1 and R2. R2 houses a diferric-tyrosyl radical (Y•) cofactor. Saccharomyces cerevisiae has two R2s: Y2 (β2) and Y4 (β′2). Y4 is an unusual R2 because three residues required for iron binding have been mutated. While the heterodimer (ββ′) is thought to be the active form, several rnr4Δ strains are viable. To resolve this paradox, N-terminally epitope-tagged β and β′ were expressed in E. coli or integrated into the yeast genome. In vitro exchange studies reveal that when apo-(His6)-β2 (Hisβ2) is mixed with β′2, apo-Hisββ′ forms quantitatively within 2 min. In contrast, holo-ββ′ fails to exchange with apo-Hisβ2 to form holo-Hisββ and β′2. Isolation of genomically encoded tagged β or β′ from yeast extracts gave a 1:1 complex of β and β′, suggesting that ββ′ is the active form. The catalytic activity, protein concentrations, and Y• content of the rnr4Δ and wild type (wt) strains were compared to clarify the role of β′ in vivo. The Y• content of rnr4Δ is 15-fold less than that of wt, consistent with the observed low activity of rnr4Δ extracts (<0.01 nmol min−1 mg−1) versus wt (0.06 ± 0.01 nmol min−1 mg−1). FLAGβ2 isolated from the rnr4Δ strain has a specific activity of 2 nmol min−1 mg−1, similar to that of reconstituted apo-Hisβ2 (10 nmol min−1 mg−1), but significantly less than holo-Hisββ′ (~2000 nmol min−1 mg−1). These studies together demonstrate that β′ plays a crucial role in cluster assembly in vitro and in vivo and that the active form of the yeast R2 is ββ′. PMID:16285741

  14. Antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi.

    PubMed

    Felczykowska, Agnieszka; Pastuszak-Skrzypczak, Alicja; Pawlik, Anna; Bogucka, Krystyna; Herman-Antosiewicz, Anna; Guzow-Krzemińska, Beata

    2017-06-07

    Lichens that were used in traditional medicine for ages produce numerous secondary metabolites, however our knowledge about biological activities of substances secreted by separated bionts is scarce. The main objectives of this study were to isolate and find optimal conditions for the growth of mycelia from three common lichen-forming fungi, i.e. Caloplaca pusilla, Protoparmeliopsis muralis and Xanthoria parietina and to evaluate antibacterial and antiproliferative activities of their acetone extracts. Agar disc diffusion and broth microdilution methods were used to test antimicrobial activity against six species of bacteria. MTT method, flow cytometry assay and DAPI staining were applied to test antiproliferative activity of selected extracts against MCF-7 (human breast adenocarcinoma), PC-3 (human prostate cancer) and HeLa (human cervix adenocarcinoma) cancer cells. P. muralis strongly inhibited the growth of Gram-positive bacteria, i.e. Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis (MICs from 6.67 to 100.00 μg mL -1 ). X. parietina grown on PDA and G-LBM media decreased HeLa or MCF-7 cancer cells viability with IC 50 values of about 8 μg mL -1 , while C. pusilla grown on G-LBM medium showed the highest potency in decreasing MCF-7 (7.29 μg mL -1 ), PC-3 (7.96 μg mL -1 ) and HeLa (6.57 μg mL -1 ) cancer cells viability. We also showed induction of apoptosis in HeLa, PC-3 and MCF-7 cell lines treated with increasing concentrations of C. pusilla extract. We showed that selected acetone extracts demonstrated a strong antimicrobial and anticancer effects that suggests that aposymbiotically cultured lichen-forming fungi can be a source of antibacterial and antiproliferative compounds.

  15. 75 FR 66079 - National Grid USA; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-27

    ... 11013 (Feb. 29, 2008), FERC Stats. & Regs. ] 31,264, at P 4-5, order on rehearing, Order No. 707-A, 73 FR 43072 (Jul. 24, 2008), FERC Stats. & Regs. ] 31,272 (2008). Any person desiring to intervene or to...

  16. 75 FR 34108 - National Grid USA; Notice of Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    .... 707, 73 FR 11013 (Feb. 29, 2008), FERC Stats. & Regs. ] 31,264, at P 4-5, order on rehearing, Order No. 707-A, 73 FR 43072 (Jul. 24, 2008), FERC Stats. & Regs. ] 31,272 (2008). Any person desiring to...

  17. 78 FR 34091 - Sierrita Gas Pipeline LLC; Notice of Public Meeting for the Proposed Sierrita Pipeline Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... Sierrita Pipeline Project right-of-way. The meeting is open to the public; however, the intent of the..., or on the FERC Web site at www.ferc.gov using the ``eLibrary'' link. Click on the eLibrary link...

  18. 77 FR 61776 - Agency Information Collection Activities: Petition for Alien Fiancé(e), Form I-129F, Revision of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-11

    ...-0001] Agency Information Collection Activities: Petition for Alien Fianc[eacute](e), Form I-129F.../Collection: Petition for Alien Fianc[eacute](e). (3) Agency form number, if any, and the applicable component... United States in order to petition for an alien fianc[eacute](e), spouse, or his/her children. (5) An...

  19. Fall prevention with supplemental and active forms of vitamin D: a meta-analysis of randomised controlled trials

    PubMed Central

    Dawson-Hughes, B; Staehelin, H B; Orav, J E; Stuck, A E; Theiler, R; Wong, J B; Egli, A; Kiel, D P; Henschkowski, J

    2009-01-01

    Objective To test the efficacy of supplemental vitamin D and active forms of vitamin D with or without calcium in preventing falls among older individuals. Data sources We searched Medline, the Cochrane central register of controlled trials, BIOSIS, and Embase up to August 2008 for relevant articles. Further studies were identified by consulting clinical experts, bibliographies, and abstracts. We contacted authors for additional data when necessary. Review methods Only double blind randomised controlled trials of older individuals (mean age 65 years or older) receiving a defined oral dose of supplemental vitamin D (vitamin D3 (cholecalciferol) or vitamin D2 (ergocalciferol)) or an active form of vitamin D (1α-hydroxyvitamin D3 (1α-hydroxycalciferol) or 1,25-dihydroxyvitamin D3 (1,25-dihydroxycholecalciferol)) and with sufficiently specified fall assessment were considered for inclusion. Results Eight randomised controlled trials (n=2426) of supplemental vitamin D met our inclusion criteria. Heterogeneity among trials was observed for dose of vitamin D (700-1000 IU/day v 200-600 IU/day; P=0.02) and achieved 25-hydroxyvitamin D3 concentration (25(OH)D concentration: <60 nmol/l v ≥60 nmol/l; P=0.005). High dose supplemental vitamin D reduced fall risk by 19% (pooled relative risk (RR) 0.81, 95% CI 0.71 to 0.92; n=1921 from seven trials), whereas achieved serum 25(OH)D concentrations of 60 nmol/l or more resulted in a 23% fall reduction (pooled RR 0.77, 95% CI 0.65 to 0.90). Falls were not notably reduced by low dose supplemental vitamin D (pooled RR 1.10, 95% CI 0.89 to 1.35; n=505 from two trials) or by achieved serum 25-hydroxyvitamin D concentrations of less than 60 nmol/l (pooled RR 1.35, 95% CI 0.98 to 1.84). Two randomised controlled trials (n=624) of active forms of vitamin D met our inclusion criteria. Active forms of vitamin D reduced fall risk by 22% (pooled RR 0.78, 95% CI 0.64 to 0.94). Conclusions Supplemental vitamin D in a dose of 700-1000 IU a day

  20. Effect of human serum albumin upon the permeabilizing activity of sticholysin II, a pore forming toxin from Stichodactyla heliantus.

    PubMed

    Celedón, Gloria; González, Gustavo; Gulppi, Felipe; Pazos, Fabiola; Lanio, María E; Alvarez, Carlos; Calderón, Cristian; Montecinos, Rodrigo; Lissi, Eduardo

    2013-12-01

    Sticholysin II (St II) is a haemolytic toxin isolated from the sea anemone Stichodactyla helianthus. The high haemolytic activity of this toxin is strongly dependent on the red cell status and the macromolecule conformation. In the present communication we evaluate the effect of human serum albumin on St II haemolytic activity and its capacity to form pores in the bilayer of synthetic liposomes. St II retains its pore forming capacity in the presence of large concentrations (up to 500 μM) of human serum albumin. This effect is observed both in its capacity to produce red blood cells haemolysis and to generate functional pores in liposomes. In particular, the capacity of the toxin to lyse red blood cells increases in the presence of human serum albumin (HSA). Regarding the rate of the pore forming process, it is moderately decreased in liposomes and in red blood cells, in spite of an almost total coverage of the interface by albumin. All the data obtained in red cells and model membranes show that St II remains lytically active even in the presence of high HSA concentrations. This stubbornness can explain why the toxin is able to exert its haemolytic activity on membranes immersed in complex plasma matrixes such as those present in living organisms.

  1. 75 FR 18203 - Desert Southwest Power, LLC; Notice of Petition for Declaratory Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-09

    ... Reform, Order No. 679, 71 FR 43294 (July 31, 2006), FERC Stats. & Regs. ] 31,222 (2006), order on reh'g, Order No. 679-A, 72 FR 1152 (Jan. 10, 2007), FERC Stats. & Regs. ] 31,236 (2006), order on reh'g, 119...

  2. ACHP | News

    Science.gov Websites

    Chairman of FERC in reaching his final decision on the project. The Chairman of FERC must take into account these comments and respond to them prior to reaching a decision on the project. Read the ACHP's comments

  3. Biofilm-forming activity of bacteria isolated from toilet bowl biofilms and the bactericidal activity of disinfectants against the isolates.

    PubMed

    Mori, Miho; Gomi, Mitsuhiro; Matsumune, Norihiko; Niizeki, Kazuma; Sakagami, Yoshikazu

    2013-01-01

    To evaluate the sanitary conditions of toilets, the bacterial counts of the toilet bowl biofilms in 5 Kansai area and 11 Kansai and Kanto area homes in Japan were measured in winter and summer seasons, respectively. Isolates (128 strains) were identified by analyzing 16S ribosomal RNA sequences. The number of colonies and bacterial species from biofilms sampled in winter tended to be higher and lower, respectively, than those in summer. Moreover, the composition of bacterial communities in summer and winter samples differed considerably. In summer samples, biofilms in Kansai and Kanto areas were dominated by Blastomonas sp. and Mycobacterium sp., respectively. Methylobacterium sp. was detected in all toilet bowl biofilms except for one sample. Methylobacterium sp. constituted the major presence in biofilms along with Brevundimonas sp., Sphingomonas sp., and/or Pseudomonas sp. The composition ratio of the sum of their genera was 88.0 from 42.9% of the total bacterial flora. The biofilm formation abilities of 128 isolates were investigated, and results suggested that Methylobacterium sp. and Sphingomonas sp. were involved in biofilm formation in toilet bowls. The biofilm formation of a mixed bacteria system that included bacteria with the highest biofilm-forming ability in a winter sample was greater than mixture without such bacteria. This result suggests that isolates possessing a high biofilm-forming activity are involved in the biofilm formation in the actual toilet bowl. A bactericidal test against 25 strains indicated that the bactericidal activities of didecyldimethylammonium chloride (DDAC) tended to be higher than those of polyhexamethylene biguanide (PHMB) and N-benzyl-N,N-dimethyldodecylammonium chloride (ADBAC). In particular, DDAC showed high bactericidal activity against approximately 90% of tested strains under the 5 h treatment.

  4. 75 FR 45626 - PJM Interconnection, L.L.C.; Notice of Petition for Declaratory Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... review in the Commission's Public Reference Room in Washington, DC. There is an ``eSubscription'' link on... participation in PJM's markets are miniscule in relation to their overall business activities. Any person... subscribed docket(s). For assistance with any FERC Online service, please e-mail [email protected

  5. The Essay: Theory and Pedagogy for an Active Form.

    ERIC Educational Resources Information Center

    Heilker, Paul

    Calling for a radical reexamination of the traditional foundation of composition instruction--the thesis/support form, this book argues that the essay, with its informality, conversational tone, meditative mood, and integration of form and content, is better suited to developmental, epistemological, ideological, and feminist rhetorical…

  6. 77 FR 12832 - Non-RTO/ISO Performance Metrics; Commission Staff Request Comments on Performance Metrics for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-02

    ...- peak hours; and (4) additional information on equipment types affected and kV of lines affected. Items... Regulatory Commission (Commission or FERC), among other actions, work with regional transmission... Average burden FERC-922 requirements respondents responses per hours per Total annual annually respondent...

  7. Environmental bacteriophages active on biofilms and planktonic forms of toxigenic Vibrio cholerae: Potential relevance in cholera epidemiology.

    PubMed

    Naser, Iftekhar Bin; Hoque, M Mozammel; Abdullah, Ahmed; Bari, S M Nayeemul; Ghosh, Amar N; Faruque, Shah M

    2017-01-01

    Phages isolated from environmental waters in Bangladesh were tested for their host specificity towards V. cholerae O1 and O139, and the ability to disperse V. cholerae biofilms formed in the laboratory. Representative phages were further characterized by electron microscopy and whole genome sequencing. Selected phages were then introduced in various combinations to biofilms of toxigenic V. cholerae added to samples of river water, and the dispersion of biofilms as well as the growth kinetics of V. cholerae and the phages were monitored. A phage cocktail composed of three different phages isolated from surface waters in Bangladesh and designated as JSF7, JSF4, and JSF3 could significantly influence the distribution and concentration of the active planktonic form and biofilm associated form of toxigenic V. cholerae in water. While JSF7 showed a biofilm degrading activity and dispersed cells from both V. cholerae O1 and O139 derived biofilms thus increasing the concentration of planktonic V. cholerae in water, JSF4 and JSF3 showed strong bactericidal activity against V. cholerae O1 and O139 respectively. A mixture of all three phages could effectively reduce both biofilm-associated and planktonic V. cholerae in river water microcosms. Besides potential applicability in phage-mediated control of cholera, our results have relevance in appreciating possible intricate role of diverse environmental phages in the epidemiology of the disease, since both biofilms and phages influence the prevalence and infectivity of V. cholerae in a variety of ways.

  8. Analysis of PURPA and solar energy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rice, M.

    The Public Utility Regulatory Policies Act of 1978 (PURPA) is designed to promote energy conservation, the efficient use of utility resources, and equitable rates. PURPA specifically directs the Federal Energy Regulatory Commission (FERC) to encourage small power production from renewable resources (and also cogeneration of electric energy as well as heat) by setting standards under which facilities qualify for interconnection, and guidelines for sales between utilities and independent facilities. The way FERC carries out this mandate may critically affect the development of solar alternatives to electric power production from fossil and nuclear resources. This report comments on proposed FERC regulationsmore » and suggests ways to encourage small power production within the PURPA mandate. In addition, some internal strains within PURPA are analyzed that seem to limit the effectiveness with which FERC can encourage independent facilities, and possible modifications to PURPA are suggested. 255 references.« less

  9. Intracellular serine protease 1 of Bacillus subtilis is formed in vivo as an unprocessed, active protease in stationary cells.

    PubMed Central

    Sheehan, S M; Switzer, R L

    1990-01-01

    Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the protease was found. Images FIG. 1 PMID:2104610

  10. 76 FR 41279 - Agency Information Collection Activities; Form I-864, Form I-864A, Form I-864EZ, and Form I-864W...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-13

    ... Immigrant's Affidavit of Support Exemption. The Department of Homeland Security, U.S. Citizenship and.... These forms are used by family-based and certain employment-based immigrants to have the petitioning...

  11. Assessing the relationship between quality of life and behavioral activation using the Japanese Behavioral Activation for Depression Scale-Short Form.

    PubMed

    Shudo, Yusuke; Yamamoto, Tatsuya

    2017-01-01

    Quality of life (QOL) is an important health-related concept. Identifying factors that affect QOL can help develop and improve health-promotion interventions. Previous studies suggest that behavioral activation fosters subjective QOL, including well-being. However, the mechanism by which behavioral activation improves QOL is not clear. Considering that QOL improves when depressive symptoms improve post-treatment and that behavioral activation is an effective treatment for depression, it is possible that behavioral activation affects QOL indirectly rather than directly. To clarify the mechanism of the influence of behavioral activation on QOL, it is necessary to examine the relationships between factors related to behavioral activation, depressive symptoms, and QOL. Therefore, we attempted to examine the relationship between these factors. Participants comprised 221 Japanese undergraduate students who completed questionnaires on behavioral activation, QOL, and depressive symptoms: the Japanese versions of the Behavioral Activation for Depression Scale-Short Form (BADS-SF), WHO Quality of Life-BREF (WHOQOL-26), and Center for Epidemiologic Studies Depression Scale (CES-D). The BADS-SF comprises two subscales, Activation and Avoidance, and the WHOQOL-26 measures overall QOL and four domains, Physical Health, Psychological Health, Social Relationships, and Environment. Mediation analyses were conducted with BADS-SF activation and avoidance as independent variables, CES-D as a mediator variable, and each WHO-QOL as an outcome variable. Results indicated that depression completely mediated the relationship between Avoidance and QOL, and partially mediated the relationship between Activation and QOL. In addition, analyses of each domain of QOL showed that Activation positively affected all aspects of QOL directly and indirectly, but Avoidance had a negative influence on only part of QOL mainly through depression. The present study provides behavioral activation strategies

  12. Adhesive Properties and Acid-Forming Activity of Lactobacilli and Streptococci Under Inhibitory Substances, Such as Nitrates.

    PubMed

    Hakobyan, L; Harutyunyan, K; Harutyunyan, N; Melik-Andreasyan, G; Trchounian, A

    2016-06-01

    One of the main requirements for probiotics is their ability to survive during passage through gastrointestinal tract and to maintain their activity at different adverse conditions. The aim of the study was to look for the strains of lactobacilli and streptococci with high adhesive properties even affected by inhibitory substances, such as nitrates (NO3 (-)). To study the adhesion properties hemagglutination reaction of bacterial cells with red blood cells of different animals and humans was used. The acid formation ability of bacteria was determined by the method of titration after 7 days of incubation in the sterile milk. These properties were investigated at different concentrations of NO3 (-). The high concentration (mostly ≥2.0 %) NO3 (-) inhibited the growth of both lactobacilli and streptococci, but compared with streptococcal cultures lactobacilli, especially Lactobacillus acidophilus Ep 317/402, have shown more stability and higher adhesive properties. In addition, the concentrations of NO3 (-) of 0.5-2.0 % decreased the acid-forming activity of the strains, but even under these conditions they coagulated milk and, in comparison to control, formed low acidity in milk. Thus, the L. acidophilus Ep 317/402 with high adhesive properties has demonstrated a higher activity of NO3 (-) transformation.

  13. Isolation of a latent polyphenol oxidase from loquat fruit (Eriobotrya japonica Lindl.): kinetic characterization and comparison with the active form.

    PubMed

    Sellés-Marchart, Susana; Casado-Vela, Juan; Bru-Martínez, Roque

    2006-02-15

    Polyphenol oxidase (PPO) has been extracted from both soluble and particulate fractions of loquat fruit (Eriobotrya japonica Lindl. cv. Algerie). The soluble PPO (20% of total activity) was partially purified 3.3-fold after ammonium sulfate fractionation being in its active state. The particulate PPO fraction (80% of total activity) was purified to homogeneity in a latent form being activable by sodium dodecyl sulfate (SDS). The enzyme was purified 40.0-fold with a total yield of 15.3% after extraction by phase partitioning in Triton X-114 followed by three chromatographic steps. The molecular weight was estimated to be about 59.2 and 61.2 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography, respectively, indicating that latent PPO is a monomer. Latent PPO catalyzed the oxidation of chlorogenic acid (CA) at a rate 50-fold faster than that of 4-tert-butylcatechol (TBC) but the soluble active counterpart only twice. Both PPOs exhibited similar Km values for TBC but Km for CA was 5-fold higher for the latent than for the active soluble PPO. Other kinetic characteristics, including sensitivity to inhibitors, substrate specificity, thermal stability, temperature, and pH profiles, were quite different between both PPOs. These results provide strong evidences that the soluble active and the particulate latent are different forms of PPO in loquat fruit flesh. The results suggest that the major PPO form for the oxidation of CA, leading to enzymatic browning under physiological conditions, is the latent one.

  14. [Hemorrhagic and edema-forming activity and histologic changes in the mouse footpad induced by venoms from Argentinian Bothrops and Crotalus genuses].

    PubMed

    Acosta de Pérez, O C; Koscinczuk, P; Teibler, P; Sánchez Negrette, M; Ruiz, R; Maruñak, S; Bogarín, G

    1998-08-01

    Hemorrhagic, oedema-forming activities and histopathological alterations in the mouse footpad induced by Bothrops and Crotalus snake venoms from Argentina. Hemorrhagic and oedema-forming activities of various Bothrops and Crotalus snake venoms from Argentina were studied, together with histological alterations in the mouse footpad. The highest oedema-forming activity was found in the venom of B. jararaca, followed by B. jararacussu, B. neuwiedii diporus, B. alternatus, and Crotalus durissus terrificus. Regarding hemorrhage, the highest activity was found in the venom of B. neuwiedii diporus, followed by B. jararacussu, B. alternatus, and B. jararaca. No hemorrhage was observed after injection of Crotalus durissus terrificus venom, in agreement with histological observations of injected footpads. Histological analysis revealed a conspicuous inflammatory reaction in the injected footpad, characterized by oedema and an inflammatory infiltrate rich in polymorphonuclear leucocytes. Necrotic blood vessels and dilated lymphatic vessels were observed after injection of B. jararaca and B. jararacussu venoms, and myonecrosis was evident in tissue of mice injected with B. alternatus and B. neuwiedii diporus.

  15. 77 FR 13121 - Solar Energy Industries Association: Notice of Petition for Rulemaking

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-05

    ... its small generator interconnection rules and procedures \\1\\ for solar electric generation. \\1\\ Standardization of Small Generator Interconnection Agreements and Procedures, Order No. 2006, FERC Stats. & Regs... First Street NE., Washington, DC 20426. This filing is accessible on-line at http://www.ferc.gov , using...

  16. 78 FR 55249 - Transmission Relay Loadability Reliability Standard; Notice of Compliance Filing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-10

    ...; RM11-16-000] Transmission Relay Loadability Reliability Standard; Notice of Compliance Filing Take.... \\1\\ Transmission Relay Loadability Reliability Standard, Order No. 733, 130 FERC ] 61, 221 (2010..., Order No. 733-B, 136 FERC ] 61,185 (2011). \\2\\ Transmission Relay Loadability Reliability Standard, 138...

  17. La forme en -l en macedonien (The Form Suffixed -1 in Macedonian).

    ERIC Educational Resources Information Center

    Hristova, Doreana

    The Macedonian verb form corresponding to the form ending in "-l" in French is examined, focusing on the active past participle, which represents the past indeterminate or non-testimonial tense. Special attention is paid to aspectual, modal, temporal, and prosodic values, and all examples are drawn from the two languages. (MSE)

  18. A not-so-funny thing happened on the way to relicensing the Edwards Dam

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ayer, F.J.; Isaacson, M.

    1995-12-31

    What started out as a seemingly straightforward and simple exercise, obtaining a new FERC license for the Edwards Dam in Augusta, Maine, turned out to be anything but straightforward and far from simple. This article tells the story of one of the more interesting and possibly precedent setting cases in the {open_quotes}class of 93{close_quotes} and is presented in three sections: (1) the history of the Edwards Dam and the FERC regulatory process through the spring of 1995; (2) Edwards` response to the dam removal campaign; and (3) recommendations for FERC licensees threatened by dam removal during relicensing.

  19. 78 FR 66350 - Commission Information Collection Activities (FERC-574); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... State Commission is exercising that jurisdiction. 18 CFR Part 152 specifies the data required to be... Cost per FTE, including salary + benefits. Comments: Comments are invited on: (1) Whether the...

  20. 77 FR 73630 - Commission Information Collection Activities (FERC-732); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ... Transmission Rights in Organized Electricity Markets) to the Office of Management and Budget (OMB) for review... Firm Transmission Rights in Organized Electricity Markets. OMB Control No.: 1902-0245. Type of Request... or more organized electricity markets to make available long-term firm transmission rights that...

  1. 78 FR 16486 - Commission Information Collection Activities (FERC-606); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ...-time employment). \\4\\ Average salary plus benefits per full-time equivalent employee. Comments... Requests for Further Information), to the Office of Management and Budget (OMB) for review of the... intended to allow agencies to assist the Commission to make better informed decisions in establishing due...

  2. 77 FR 31607 - Commission Information Collection Activities (FERC-587); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... improvement of navigation and for the development, transmission, and utilization of power across, along, from... applicable U.S. land. \\1\\ 16 U.S.C. 797d (2010). \\2\\ Public Law 99-495, 100 Stat. 1243 (1996). The... of information technology. Dated: May 21, 2012. Kimberly D. Bose, Secretary. [FR Doc. 2012-12869...

  3. 77 FR 40347 - Commission Information Collection Activities (FERC-552); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-09

    ... Transactions Number of Average burden Number of responses per Total number of hours per Estimated total... facilitate price transparency in markets for the sale or transportation of physical natural gas in interstate... market participants. Estimate of Annual Burden \\3\\: The Commission estimates the total Public Reporting...

  4. 78 FR 77111 - Commission Information Collection Activities (FERC-555); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-20

    ... figures due to current cost information and differences in the total number of respondents. \\4\\ The.... The Commission issued a Notice in the Federal Register (78 FR 61983, 10/9/2013) requesting public..., natural gas and oil pipeline companies subject to the Commission's jurisdiction. Regulated entities use...

  5. 78 FR 63974 - Commission Information Collection Activities (FERC-547); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-25

    ... gas companies 11 1 11 75 825 The total estimated annual cost burden to respondents is $57,750. [825... comments to the Commission as explained below. The Commission issued a Notice in the Federal Register (78... Commission to order a refund (with interest) for any portion of a natural gas company's increased rate or...

  6. 78 FR 47310 - Commission Information Collection Activities (FERC-582); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-05

    ... energy in interstate commerce. Respondents (public utilities, power marketers) subject to these annual... charge. \\6\\ 18 CFR 381 and 382. Type of Respondents: Public utilities and power marketers. Estimate of...

  7. 77 FR 69615 - Commission Information Collection Activities (FERC-715); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-20

    ... transmission planning; A detailed description of the transmission planning reliability criteria used to..., but not limited to, how reliability criteria are applied and the steps taken in [[Page 69616... performance as measured against its stated reliability criteria using its stated assessment practices. The...

  8. 77 FR 69614 - Commission Information Collection Activities (FERC-714); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-20

    ... to correlate rates and charges, assess reliability and other operating attributes in regulatory..., including the validity of the methodology and assumptions used; (3) ways to enhance the quality, utility and...

  9. 77 FR 35667 - Commission Information Collection Activities (FERC-567); Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... Act of 1995, 44 United States Code (U.S.C.) 3507(a)(1)(D), the Federal Energy Regulatory Commission... Reports of System Flow Diagrams and System Capacity to the Office of Management and Budget (OMB) for... System Flow Diagrams and System Capacity. OMB Control No.: 1902-0005. Type of Request: Three-year...

  10. Negotiating Terms and Conditions: An Overview of the Federal Energy Regulatory Commission Hydropower Settlement Agreement Process

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Levine, Aaron L; Curtis, Taylor L; Shields, Laura

    The process for obtaining, transferring, or surrendering a Federal Energy Regulatory Commission (FERC) hydropower license or exemption from licensing can be a series of complex negotiations. In many instances, the process may include stakeholders with different and/or competing perspectives or objectives related to the original license, new license (i.e., relicense), transfer of a license, or surrender of a license. Divergent perspectives and objectives can lead to disputes among project participants, which may impact the development of FERC license terms and conditions. One mechanism to resolve disputes between project participants involving a FERC authorization is informal and formal settlement agreement conferences,more » which often result in settlement agreements addressing the disputed issues. This report provides hydropower stakeholders with a better understanding of the FERC settlement agreement process, including information on: How to initiate a settlement agreement. The procedural steps associated with the settlement agreement process. The legal standards the Commission must adhere to when determining whether settlement agreement provisions can become lawful and enforceable terms or conditions of a FERC authorization. Examples of settlement agreement provisions included as license terms and conditions or in off-license agreements from a collection of settlement agreements and Commission orders.« less

  11. Forms of selenium affect its transport, uptake and glutathione peroxidase activity in the Caco-2 cell model.

    PubMed

    Wang, Yanbo; Fu, Linglin

    2012-10-01

    The experiment was designed to investigate the effect of selenium (Se) chemical forms (sodium selenite, selenium nanoparticle [nano-Se] and selenomethionine) on the transport, uptake and glutathione peroxidase (GSH-Px) activity in the Caco-2 cell model. The transport and uptake of different forms of Se (0.1 μmol l(-1)) across the Caco-2 cell monolayer were carried out in two directions (apical [AP] to basolateral [BL] and BL to AP) for 2 h, respectively, and the apparent permeability coefficient (P(app)), transport efficiency and uptake efficiency were all calculated. In the present study, the transport and uptake of three forms of Se were time-dependent both in AP to BL and BL to AP directions. By the end of 2 h, the transport efficiencies of selenomethionine and nano-Se were higher than that of sodium selenite (P<0.05). The highest uptake efficiency (P<0.05) was observed in cells treated with nano-Se and significant difference (P<0.05) was also observed between the cells incubated with sodium selenite and selenomethionine. As for the P(app), sodium selenite (P<0.05) had the lowest values compared with that of selenomethionine and nano-Se, in both AP-BL and BL-AP. However, no significant differences were observed in GSH-Px activities. These results indicated that the efficiency of Se in the Caco-2 cells varied with its chemical forms, which might be associated with the differences in Se transport and uptake.

  12. Nicolaus Taurellus on forms and elements.

    PubMed

    Blank, Andreas

    2014-12-01

    This article examines the conception of elements in the natural philosophy of Nicolaus Taurellus (1547-1606) and explores the theological motivation that stands behind this conception. By some of his early modern readers, Taurellus may have been understood as a proponent of material atoms. By contrast, I argue that considerations concerning the substantiality of the ultimate constituents of composites led Taurellus to an immaterialist ontology, according to which elements are immaterial forms that possess active and passive potencies as well as motion and extension. In Taurellus's view, immaterialism about elements provides support for the theological doctrine of creation ex nihilo. As he argues, the ontology of immaterial forms helps to explicate a sense in which creatures are substances, not accidents of the divine substance. In particular, he maintains that immaterial forms stand in suitable relations of ontological dependence to God: creation dependence (since forms would not exist without the divine act of creation), but neither subsistence dependence (since forms continue to exist without continued divine agency) nor activity dependence (since forms are active without requiring divine concurrence).

  13. 76 FR 22691 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-22

    ... Power V, LLC, First Wind Energy Marketing, LLC, Milford Wind Corridor Phase I, LLC, Milford Wind...-certification [or self-recertification] listed above, do not institute a proceeding regarding qualifying... lieu of paper, using the FERC Online links at http://www.ferc.gov . To facilitate electronic service...

  14. 76 FR 46791 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... Trading and Marketing, LLC, Duke Energy Vermillion II, LLC, Duke Energy Washington II, LLC, Happy Jack...] listed above, do not institute a proceeding regarding qualifying facility status. A notice of self... the FERC Online links at http://www.ferc.gov . To facilitate electronic service, persons with Internet...

  15. 75 FR 61742 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ...- 1255-004. Applicants: ArcLight Energy Marketing, LLC, Oak Creek Wind Power, LLC, Coso Geothermal Power...] listed above, do not institute a proceeding regarding qualifying facility status. A notice of self... the FERC Online links at http://www.ferc.gov . To facilitate electronic service, persons with Internet...

  16. 76 FR 17409 - Combined Notice of Filings #

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-29

    ... Energy Marketing Company Description: Ameren Energy Marketing Company submits tariff filing per 35.13(a... of self-certification [or self-recertification] listed above, do not institute a proceeding regarding... interventions in lieu of paper, using the FERC Online links at http://www.ferc.gov . To facilitate electronic...

  17. 76 FR 19347 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-07

    ...: Notice of Cancellation of Interchange Agreement with LG&E Energy Marketing by Florida Power Corporation...-recertification] listed above, do not institute a proceeding regarding qualifying facility status. A notice of... the FERC Online links at http://www.ferc.gov . To facilitate electronic service, persons with Internet...

  18. 75 FR 8691 - Combined Notice of Filings # 3

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ..., 2010. Take notice that the Commission received the following electric rate filings: Docket Numbers.... Applicants: Xcel Energy Services Inc. Description: Northern States Power Company et al submits Original Sheet 1 et al to FERC Electric Tariff, Original Volume 3--FERC Electric Rate Schedule 280-NSP. Filed Date...

  19. 77 FR 56835 - Constitution Pipeline Company, LLC; Notice of Intent To Prepare an Environmental Impact Statement...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ...? What Do I Need To Know?'' is available for viewing on the FERC Web site ( www.ferc.gov ). This fact...; Installation of four new meter and regulation (M&R) stations including: [ssquf] Central M&R Receipt Station--a new M&R receipt station, including [[Page 56836

  20. 77 FR 53197 - Raven Power Marketing LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-31

    ... Marketing LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for Blanket... Marketing LLC's application for market-based rate authority, with an accompanying rate schedule, noting that... interventions in lieu of paper, using the FERC Online links at http://www.ferc.gov . To facilitate electronic...

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