Science.gov

Sample records for activities low-energy marine

  1. 78 FR 33811 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-05

    ... exclusion zones for the airgun array to be used in the intermediate and deep water of the Gulf of Mexico... in deep water; however, SIO proposes to use the buffer and exclusion zones predicted by L-DEO's model... Specified Activities; Low- Energy Marine Geophysical Survey in the Tropical Western Pacific Ocean,...

  2. 77 FR 19242 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-30

    ...NMFS has received an application from Lamont-Doherty Earth Observatory (L-DEO), a part of Columbia University, for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to conducting a low-energy marine geophysical survey in the central Pacific Ocean, May through June, 2012. Pursuant to the Marine Mammal Protection Act (MMPA), NMFS is requesting......

  3. 78 FR 11821 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-20

    ... Seismic Survey by the U.S. Geological Survey in the Deepwater Gulf of Mexico, April-May 2013'' (EA). USGS... the U.S. Geological Survey in the Northwestern Gulf of Mexico, April-May 2013,'', prepared by LGL Ltd... conducting a low-energy marine seismic survey within the U.S. Exclusive Economic Zone in the deep water...

  4. 76 FR 68720 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-07

    ... slope, 13,620 to 18,680 EN D 0 physalus). pelagic. \\9\\. 0 0 Blue whale (Balaneoptera Pelagic, shelf, NA... published a notice in the Federal Register (76 FR 45518) making preliminary determinations and proposing to... previous notice for the proposed IHA (76 FR 45518, July 29, 2011). The activities to be conducted have...

  5. Mariner-Jupiter-Saturn Low Energy Charged Particle Experiment

    NASA Technical Reports Server (NTRS)

    Peletier, D. P.; Gary, S. A.; Hogrefe, A. F.

    1977-01-01

    The Low Energy Charged Particle Experiment will be launched on the Mariner-Jupiter-Saturn spacecraft in August 1977. The experiment has been designed to perform particle measurements in the intense radiation belts of the Jovian and Saturnian environments, and to provide detailed spectral analysis of both solar and galactic particles in interplanetary space. A single instrument uses 23 solid-state detectors configured in two distinct detector subsystems: one optimized for interplanetary and interstellar measurements, the other for specific particle species, energies, and intensities expected near the planets.

  6. Researchers evaluate low-energy recirculating system for inland production of marine finfish juveniles

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The low-energy recirculating aquaculture system consists of nine separate modules which utilize the double drain fish culture tank paired to a moving bed biofilter. The nine fiberglass tanks are five feet in diameter and normal water depth is about three feet for a total tank volume of approximately...

  7. Low Energy Physical Activity Recognition System on Smartphones

    PubMed Central

    Morillo, Luis Miguel Soria; Gonzalez-Abril, Luis; Ramirez, Juan Antonio Ortega; de la Concepcion, Miguel Angel Alvarez

    2015-01-01

    An innovative approach to physical activity recognition based on the use of discrete variables obtained from accelerometer sensors is presented. The system first performs a discretization process for each variable, which allows efficient recognition of activities performed by users using as little energy as possible. To this end, an innovative discretization and classification technique is presented based on the χ2 distribution. Furthermore, the entire recognition process is executed on the smartphone, which determines not only the activity performed, but also the frequency at which it is carried out. These techniques and the new classification system presented reduce energy consumption caused by the activity monitoring system. The energy saved increases smartphone usage time to more than 27 h without recharging while maintaining accuracy. PMID:25742171

  8. Low energy, low latitude wave-dominated shallow marine depositional systems: examples from northern Borneo

    NASA Astrophysics Data System (ADS)

    Lambiase, Joseph J.; Suraya Tulot

    2013-12-01

    The depositional environments of the wave-dominant successions in the middle to late Miocene Belait and Sandakan Formations in northwestern and northern Borneo, respectively, were determined based on grain size distributions, sedimentary structures and facies successions, as well as trace and microfossil assemblages. Generally, progradational shoreface successions in the Belait Formation were deposited in very low wave energy environments where longshore currents were too weak to generate trough cross-bedding. Shoreface sands are laterally continuous for several km and follow the basin contours, suggesting attached beaches similar to the modern Brunei coastline. In contrast, trough cross-bedding is common in the coarser Sandakan Formation and back-barrier mangrove swamp deposits cap the progradational succession as on the modern northern Dent Peninsula coastline, indicating barrier development and higher wave energy conditions than in the Belait Formation. The Borneo examples indicate that barrier systems that include significant tidal facies form under higher wave energy conditions than attached beaches with virtually no tidal facies. Also, Borneo's low latitude climate promotes back-barrier mangrove which reduces tidal exchange and reduces tidal influence relative to comparable temperate climate systems. The results of the study indicate that depositional systems on low energy, wave-dominated coasts are highly variable, as are the sand bodies and facies associations they generate.

  9. Marine Science Activities, Grade Six.

    ERIC Educational Resources Information Center

    Kolb, James A.

    This unit, one of a series designed to develop and foster an understanding of the marine environment, presents marine science activities for grade 6 students. The unit is divided into the following sections: (1) Pagoo (story of a hermit crab); (2) introduction to marine environments; (3) salt water environment; (4) sea water investigations; (5)…

  10. Controlling activation site density by low-energy far-field stimulation in cardiac tissue.

    PubMed

    Hörning, Marcel; Takagi, Seiji; Yoshikawa, Kenichi

    2012-06-01

    Tachycardia and fibrillation are potentially fatal arrhythmias associated with the formation of rotating spiral waves in the heart. Presently, the termination of these types of arrhythmia is achieved by use of antitachycardia pacing or cardioversion. However, these techniques have serious drawbacks, in that they either have limited application or produce undesirable side effects. Low-energy far-field stimulation has recently been proposed as a superior therapy. This proposed therapeutic method would exploit the phenomenon in which the application of low-energy far-field shocks induces a large number of activation sites ("virtual electrodes") in tissue. It has been found that the formation of such sites can lead to the termination of undesired states in the heart and the restoration of normal beating. In this study we investigate a particular aspect of this method. Here we seek to determine how the activation site density depends on the applied electric field through in vitro experiments carried out on neonatal rat cardiac tissue cultures. The results indicate that the activation site density increases exponentially as a function of the intracellular conductivity and the level of cell isotropy. Additionally, we report numerical results obtained from bidomain simulations of the Beeler-Reuter model that are quantitatively consistent with our experimental results. Also, we derive an intuitive analytical framework that describes the activation site density and provides useful information for determining the ratio of longitudinal to transverse conductivity in a cardiac tissue culture. The results obtained here should be useful in the development of an actual therapeutic method based on low-energy far-field pacing. In addition, they provide a deeper understanding of the intrinsic properties of cardiac cells.

  11. Controlling activation site density by low-energy far-field stimulation in cardiac tissue

    NASA Astrophysics Data System (ADS)

    Hörning, Marcel; Takagi, Seiji; Yoshikawa, Kenichi

    2012-06-01

    Tachycardia and fibrillation are potentially fatal arrhythmias associated with the formation of rotating spiral waves in the heart. Presently, the termination of these types of arrhythmia is achieved by use of antitachycardia pacing or cardioversion. However, these techniques have serious drawbacks, in that they either have limited application or produce undesirable side effects. Low-energy far-field stimulation has recently been proposed as a superior therapy. This proposed therapeutic method would exploit the phenomenon in which the application of low-energy far-field shocks induces a large number of activation sites (“virtual electrodes”) in tissue. It has been found that the formation of such sites can lead to the termination of undesired states in the heart and the restoration of normal beating. In this study we investigate a particular aspect of this method. Here we seek to determine how the activation site density depends on the applied electric field through in vitro experiments carried out on neonatal rat cardiac tissue cultures. The results indicate that the activation site density increases exponentially as a function of the intracellular conductivity and the level of cell isotropy. Additionally, we report numerical results obtained from bidomain simulations of the Beeler-Reuter model that are quantitatively consistent with our experimental results. Also, we derive an intuitive analytical framework that describes the activation site density and provides useful information for determining the ratio of longitudinal to transverse conductivity in a cardiac tissue culture. The results obtained here should be useful in the development of an actual therapeutic method based on low-energy far-field pacing. In addition, they provide a deeper understanding of the intrinsic properties of cardiac cells.

  12. A Kinematically Beamed, Low Energy Pulsed Neutron Source for Active Interrogation

    SciTech Connect

    Dietrich, D; Hagmann, C; Kerr, P; Nakae, L; Rowland, M; Snyderman, N; Stoeffl, W; Hamm, R

    2004-10-07

    We are developing a new active interrogation system based on a kinematically focused low energy neutron beam. The key idea is that one of the defining characteristics of SNM (Special Nuclear Materials) is the ability for low energy or thermal neutrons to induce fission. Thus by using low energy neutrons for the interrogation source we can accomplish three goals, (1) Energy discrimination allows us to measure the prompt fast fission neutrons produced while the interrogation beam is on; (2) Neutrons with an energy of approximately 60 to 100 keV do not fission 238U and Thorium, but penetrate bulk material nearly as far as high energy neutrons do and (3) below about 100keV neutrons lose their energy by kinematical collisions rather than via the nuclear (n,2n) or (n,n') processes thus further simplifying the prompt neutron induced background. 60 keV neutrons create a low radiation dose and readily thermal capture in normal materials, thus providing a clean spectroscopic signature of the intervening materials. The kinematically beamed source also eliminates the need for heavy backward and sideway neutron shielding. We have designed and built a very compact pulsed neutron source, based on an RFQ proton accelerator and a lithium target. We are developing fast neutron detectors that are nearly insensitive to the ever-present thermal neutron and neutron capture induced gamma ray background. The detection of only a few high energy fission neutrons in time correlation with the linac pulse will be a clear indication of the presence of SNM.

  13. Marine Science Activities, Grade Two.

    ERIC Educational Resources Information Center

    Kolb, James A.

    This unit, one of a series designed to develop and foster an understanding of the marine environment, presents marine science activities for second grade students. The unit, focusing on awareness of living/non-living factors shaping life of the sea, is divided into sections dealing with: physical characteristics of oceans; fish; sea anemone;…

  14. Improve bio-activity of anaerobic sludge by low energy ultrasound.

    PubMed

    Zhu, Yichun; Li, Xin; Du, Maoan; Liu, Zuwen; Luo, Hui; Zhang, Tao

    2015-01-01

    This research focused on ultrasound-enhanced bio-activity of anaerobic sludge. Low energy ultrasound irradiation can increase the bio-activity of anaerobic sludge. Ultrasonic parameter, characteristics of anaerobic sludge and experimental conditions are important parameters which affect the enhancement effect on anaerobic sludge. In order to assess the effects of characteristics of anaerobic sludge and experimental conditions on ultrasonic irradiation of anaerobic sludge, experiments with different characteristics of anaerobic sludge were carried out and analyzed with the content of coenzyme F420 and dehydrogenase activity (DHA). The results showed that anaerobic sludge bio-activity was impacted by the initial temperature, initial chemical oxygen demand (COD), sludge concentration, and stirring during the ultrasonic process. Optimal performance was achieved when sound frequency, power density, and ultrasonic irradiation period was 20 kHz, 0.1 W/mL, and 10 min, respectively, under which the wastewater COD removal efficiency was increased by 12.9 percentage points. The results indicated that low temperature could affect the anaerobic sludge irradiation effect, while intermittent stirring could enhance the bio-activity of anaerobic sludge irradiation effect and low substrate concentration improved anaerobic sludge activity by ultrasound. PMID:26676010

  15. Improve bio-activity of anaerobic sludge by low energy ultrasound.

    PubMed

    Zhu, Yichun; Li, Xin; Du, Maoan; Liu, Zuwen; Luo, Hui; Zhang, Tao

    2015-01-01

    This research focused on ultrasound-enhanced bio-activity of anaerobic sludge. Low energy ultrasound irradiation can increase the bio-activity of anaerobic sludge. Ultrasonic parameter, characteristics of anaerobic sludge and experimental conditions are important parameters which affect the enhancement effect on anaerobic sludge. In order to assess the effects of characteristics of anaerobic sludge and experimental conditions on ultrasonic irradiation of anaerobic sludge, experiments with different characteristics of anaerobic sludge were carried out and analyzed with the content of coenzyme F420 and dehydrogenase activity (DHA). The results showed that anaerobic sludge bio-activity was impacted by the initial temperature, initial chemical oxygen demand (COD), sludge concentration, and stirring during the ultrasonic process. Optimal performance was achieved when sound frequency, power density, and ultrasonic irradiation period was 20 kHz, 0.1 W/mL, and 10 min, respectively, under which the wastewater COD removal efficiency was increased by 12.9 percentage points. The results indicated that low temperature could affect the anaerobic sludge irradiation effect, while intermittent stirring could enhance the bio-activity of anaerobic sludge irradiation effect and low substrate concentration improved anaerobic sludge activity by ultrasound.

  16. Low-energy collisionally activated dissociation of pentose-borate complexes

    NASA Astrophysics Data System (ADS)

    Pepi, Federico; Garzoli, Stefania; Tata, Alessandra; Giacomello, Pierluigi

    2010-01-01

    Pentose-borate 1:1 complexes were generated in the ESI source of a triple quadrupole and ion trap mass spectrometer by electrospray ionization of Na2B4O7 and pentose (arabinose, lyxose, ribose, xylose) 2:1 solution in CH3CN/H2O. The study of their low-energy collisionally activated dissociation (CAD) demonstrated that ribose and lyxose are preferentially complexed at the C2-C3 cis-diol function whereas arabinose and xylose are esterified at the C1-C2 hydroxyl groups. No evidence was found of the stronger affinity for ribose to borate. The ribose probiotic rule can be explained by considering its peculiar capability, among the investigated pentoses, to almost totally complex the borate anion at the C2-C3 hydroxyl group, thus enabling the subsequent stages of nucleotide assembly, such as phosphorylation and linkage to the nucleobases. Finally, the differences observed in the pentose-borate complex CAD spectra can be used for the mass spectrometric discrimination of isomeric pentoses in complex mixtures.

  17. Optimization of low energy sonication treatment for granular activated carbon colonizing biomass assessment.

    PubMed

    Saccani, G; Bernasconi, M; Antonelli, M

    2014-01-01

    This study is aimed at optimizing a low energy sonication (LES) treatment for granular activated carbon (GAC)-colonizing biomass detachment and determination, evaluating detachment efficiency and the effects of ultrasound exposure on bacterial cell viability. GAC samples were collected from two filters fed with groundwater. Conventional heterotrophic plate count (HPC) and fluorescence microscopy with a double staining method were used to evaluate cell viability, comparing two LES procedures, without and with periodical bulk substitution. A 20 min LES treatment, with bulk substitution after cycles of 5 min as maximum treatment time, allowed to recover 87%/100% of attached biomass, protecting detached bacteria from ultrasound damaging effects. Observed viable cell inactivation rate was 6.5/7.9% cell/min, with membrane-compromised cell damage appearing to be even higher (11.5%/13.1% cell/min). Assessing bacterial detachment and damaging ultrasound effects, fluorescence microscopy turned out to be more sensitive compared to conventional HPC. The optimized method revealed a GAC-colonizing biomass of 9.9 x 10(7) cell/gGAC for plant 1 and 8.8 x 10(7) cell/gGAC for plant 2, 2 log lower than reported in literature. The difference between the two GAC-colonizing biomasses is higher in terms of viable cells (46.3% of total cells in plant 1 GAC-colonizing biomass compared to the 33.3% in plant 2). Studying influent water contamination through multivariate statistical analyses, apossible combined toxic and genotoxic effect of chromium VI and trichloroethylene was suggested as a reason for the lower viable cell fraction observed in plant 2 GAC-colonizing population.

  18. Bedrock controls of sedimentation along a marsh-dominated, open-marine, low energy coastline: west-central Florida

    SciTech Connect

    Hine, A.C.; Belknap, D.F.; Osking, E.B.; Hutton, J,G.; Evans, M.R.

    1985-01-01

    The northwestern peninsular Florida coast is microtidal, low wave energy. Topographic complexity is due to karstified Tertiary limestone, actively discharging freshwater springs, and lack of pre-Holocene sediment veneer. This complexity helps control local sedimentary processes. In the southern part of the field area a near continuous, thin Pleistocene quartz sand allows a marsh-fronting berm and simpler shoreline morphology. To the north, freshwater springs have formed shallow estuarine embayments containing linear oyster bioherms, nucleated upon local bedrock highs. These molluscan reefs enclose interbiothermal basins, segmenting the embayments. Between the embayments are archipelagoes of marsh islands and tidal creeks. Their complex topography was formed from selective dissolution along rectilinear fractures, creating linear lows which when drowned by rising sea level formed the largest tidal creeks. Marsh islands formed on intervening low-relief knobs. These commonly support oases of less salt tolerant shrubs and trees, surrounded by Juncus roemerianus marsh. Landward to seaward changes in rock/sedimentation relationships suggest an evolutionary pattern during sea-level rise. Upland forests become hammocks surrounded by marsh. Hammocks become islands surrounded by tidal creeks. Submerged hammocks finally become highs supporting oyster reefs. Preservation potential during the present slow transgression is low, due primarily to the low relief.

  19. Imaging of protein kinase C activation by FRET during proliferation induced by low-energy laser irradiation in living cells

    NASA Astrophysics Data System (ADS)

    Gao, Xuejuan; Chen, Tongsheng; Xing, Da; Wang, Fang

    2005-01-01

    Protein kinase Cs (PKCs) play an important role in cellular proliferation, and low-energy laser irradiation (LELI) can enhance cellular proliferation. The present work contributes to the understanding of the mechanisms of action by studying effects of LELI at the dose of 0.8 J/cm2 on PKCs activities in the single lung adenocarcinoma cell (ASTC-a-1) and in real time by fluorescence resonance energy transfer (FRET) technique. C-kinase activity reporter (CKAR), consisting of a cyan fluorescent protein (CFP), the FHA2 phosphothreonine-binding domain, a PKC substrate sequence, and a yellow fluorescent protein (YFP), was utilized. The living cell imaging showed a decrease in FRET in the cytosol and nucleus after the cells were treated with LELI. These results suggest that PKCs could be activated by LELI throughout the cell, and the proliferation of ASTC-a-1 cells could be modulated by the activated PKCs.

  20. Marine Science Activities for Visually Impaired.

    ERIC Educational Resources Information Center

    Schatz, Dennis; And Others

    These marine education materials are based on the approach that students learn best when given a multisensory experience. The activities are intended to develop such experiences for the visually impaired child. Activities are intended to supplement an upper-elementary science curriculum or be the basis of a unit on marine biology. The guide is…

  1. Fluxes of low-energy particles in quiet periods of solar activity and the MgII index

    NASA Astrophysics Data System (ADS)

    Zeldovich, M. A.; Logachev, Yu. I.; Kecskemety, K.; Surova, G. M.

    2009-10-01

    Low fluxes of protons with energies 0.3-10 MeV were studied during 21-23 solar cycles as a function of the MgII index using the data of the instruments CPME, EIS ( IMP8), and EPHIN ( SOHO). It has been shown that a) during quiet time of solar activity the fluxes of protons (background protons) have a positive correlation with the MgII index value throughout the solar cycle, b) specific features of variations of the MgII index during the solar minima of 1986-1987 and 1996-1997 can be considered, as well as variations of background fluxes of low energy charged particles, to be manifestations of the 22-year magnetic cycle of the Sun, and c) periods of the lowest value of the MgII index are also characterized by the smaller values of the ratio of intensities of protons and helium nuclei than in other quiet periods. A hypothesis is put forward that acceleration in a multitude of weak solar flares is one of the sources of background fluxes of low energy particles in the interplanetary space.

  2. Antibiotic activity of lectins from marine algae against marine vibrios.

    PubMed

    Liao, W-R; Lin, J-Y; Shieh, W-Y; Jeng, W-L; Huang, R

    2003-07-01

    Saline and aqueous ethanol extracts of marine algae and the lectins from two red algal species were assayed for their antibiotic activity against marine vibrios. Experimental studies were also carried out on the influence of environmental factors on such activity, using batch cultures. The results indicated that many of the saline extracts of the algal species were active and that the activity was selective against those vibrios assayed. The algal extracts were active against Vibrio pelagius and the fish pathogen V. vulnificus, but inactive against V. neresis. Algal lectins from Eucheuma serra (ESA) and Galaxaura marginata (GMA) strongly inhibited V. vulnificus but were inactive against the other two vibrios. The antibacterial activity of algal extracts was inhibited by pretreatment with various sugars and glycoprotein. Extracts of the two red algae, E. serra and Pterocladia capillacea, in saline and aqueous ethanol, inhibited markedly the growth rate of V. vulnificus at very low concentrations. Culture results indicated that metabolites active against V. vulnificus were invariably produced in P. capillacea over a wide range of temperature, light intensity, and nutritional conditions. Enhanced antibacterial activity occurred when P. capillacea was grown under higher irradiance, severe nutrient stress and moderate temperature (20 degrees C), reflecting the specific antibiotic characteristics of this alga. The strong antibiotic activity of lectins towards fish pathogenic bacteria reveals one of the important roles played by algal lectins, as well as the potential high economic value of those marine algae assayed for aquaculture and for biomedical purposes. PMID:12884128

  3. Marine Pyridoacridine Alkaloids: Biosynthesis and Biological Activities.

    PubMed

    Ibrahim, Sabrin R M; Mohamed, Gamal A

    2016-01-01

    Pyridoacridines are a class of strictly marine-derived alkaloids that constitute one of the largest chemical families of marine alkaloids. During the last few years, both natural pyridoacridines and their analogues have constituted excellent targets for synthetic works. They have been the subject of intense study due to their significant biological activities; cytotoxic, antibacterial, antifungal, antiviral, insecticidal, anti-HIV, and anti-parasitic activities. In the present review, 95 pyridoacridine alkaloids isolated from marine organisms are discussed in term of their occurrence, biosynthesis, biological activities, and structural assignment.

  4. Antifungal and antibacterial activity of marine microorganisms.

    PubMed

    El Amraoui, B; El Amraoui, M; Cohen, N; Fassouane, A

    2014-03-01

    In order to explore marine microorganisms with pharmaceutical potential, marine bacteria, collected from different coastal areas of the Moroccan Atlantic Ocean, were previously isolated from seawater, sediment, marine invertebrates and seaweeds. The antimicrobial activities of these microorganisms were investigated against the pathogens involved in human pathologies. Whole cultures of 34 marine microorganisms were screened for antimicrobial activities using the method of agar diffusion against three Gram-positive bacteria, two Gram-negative bacteria, and against yeast. The results showed that among the 34 isolates studied, 28 (82%) strains have antimicrobial activity against at least one pathogen studied, 11 (32%) strains have antifungal activity and 24 (76%) strains are active against Gram-positive bacteria, while 21 (62%) strains are active against Gram-negative bacteria. Among isolates having antimicrobial activity, 14 were identified and were assigned to the genera Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Chromobacterium, Enterococcus, Pantoea and Pseudomonas. Due to a competitive role for space and nutrient, the marine microorganisms can produce antibiotic substance; therefore, these marine microorganisms were expected to be potential resources of natural antibiotic products.

  5. Imaging of Ras/Raf activity induced by low energy laser irradiation in living cell using FRET

    NASA Astrophysics Data System (ADS)

    Wang, Fang; Chen, Tong-Sheng; Xing, Da

    2005-01-01

    Ras/Raf signaling pathway is an important signaling pathway that governs cell proliferation, differential and apoptosis. Low-energy laser irradiation (LELI) was found to modulate various processes. Generally, cell proliferation is induced by low doses LELI and apoptosis is induced by high doses LELI. Mechanism of biological effect of LELI has not been clear. Recently, activation of MEK (mitogen-activated protein kinase) and ERK (extracellular-signal-regulated kinase), which are downstream protein kinases of Ras/Raf, are observed during LELI-induced cell proliferation by immunoprecipitation and western blot analysis. RaichuRas reporter consisting of fusions of H-ras, the Ras-binding domain of Raf (RafRBD), a cyan fluorescent protein (CFP) and a yellow fluorescent protein (YFP). Therefore, intramolecular binding of GTP-Ras to RafRBD brings CFP close to YFP and increases FRET between CFP and YFP. Human lung adenocarcinoma cell line (ASTC-a-1) was transfected with the plasmid (pRaichuRas) and then treated with LELI at dose of 60J/cm2. Effect of LELI on Ras/Raf in physiological condition of living cells was observed by fluorescence resonance energy transfer (FRET) technique during lung adenocarcinoma cell apoptosis induced by high dose (60J/cm2) LELI. Experimental results showed that after high dose LELI treatment, the binding of Ras and Raf decreases obviously, Ras/Raf signaling pathway deregulates and cell apoptosis occurs.

  6. 78 FR 33369 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-04

    ... notice in the Federal Register (78 FR 11821) making preliminary determinations and proposing to issue an... outlined the purpose of the program in a previous notice for the proposed IHA (78 FR 11821, February 20... FR 11821, February 20, 2013), the IHA application, EA, and associated documents referenced above...

  7. 78 FR 57354 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-18

    ...), blue (Balaenoptera musculus), and sperm (Physeter macrocephalus) whale, as well as the dugong. Of those endangered species, the humpback, sei, fin, blue, and sperm whale is likely to be encountered in the survey... Continental slope, \\7\\ 13,620 EN D physalus). pelagic. to 18,680 Blue whale (Balaenoptera Pelagic, shelf,...

  8. 75 FR 54095 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Seismic Survey in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-03

    ...: Bryde's whale; blue whale; sperm whale; humpback whale; Cuvier's beaked whale; Blainville's beaked whale...), and blue (Balaenoptera musculus) whales. Nine cetacean species, although present in the wider ETP....53 1.15 (Balaenoptera edeni). coastal. Blue whale, (Balaenoptera Uncommon....... Pelagic and 1415...

  9. 77 FR 14744 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-13

    ... (Balaenoptera Slope, mostly \\9\\ 15,178 EN D \\5\\ 0.01 physalus). pelagic. Blue whale (Balaenoptera Pelagic and...). The density estimates were corrected for (0) and g(0). Given the higher abundance of blue whales south...\\ MMPA \\2\\ 1,000 km \\2\\) \\3\\ Mysticetes Humpback whale (Megaptera Mainly \\6\\ 2,900 EN D \\4\\...

  10. 77 FR 27189 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-09

    ... vessel surveys near the action area for dusky and Chilean dolphins as well as blue whales. Estimated...)... Slope, mostly pelagic.. \\9\\ 15,178 EN D \\5\\ 0.01 Blue whale (Balaenoptera musculus).. Pelagic and... March 13, 2012, NMFS published a notice in the Federal Register (77 FR 14744) making...

  11. 76 FR 45518 - Takes of Marine Mammals Incidental to Specified Activities; Low-Energy Marine Geophysical Survey...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-29

    ... Investigators are Drs. Masako Tominaga, Maurice A. Tivey, Daniel Lizarralde of WHOI, William W. Sager of TAMU...), and a full load draft of 5.8 m (19 ft). It is equipped with twin 360 azimuth stern thrusters...

  12. Studies on marine algae for haemagglutinic activity.

    PubMed

    Alam, M T; Usmanghani, K

    1994-07-01

    Lectins (agglutinins) are important in medical and immunological applications. Phytohaemagglutinins have been found useful in blood banking. Keeping in view of these facts, the marine algae found at Karachi coastal region have been screened for agglutinic activity by using human erythrocytes of A, B, AB and 0 group. Altogether 53 algal samples were collected and subjected to extraction, fractionation serial dilution and titre determinations. The total marine algae screened for haemagglutinic activity were 44 out of these 14, 13 and 17 belonged to Chlorophyta, Phaeophyta, and Rhodophyta respectively. Among these three groups the Rhodophyta showed the highest number of lytic activity. The green marine alga Valoniopsis pachynema showed a titre value between 2(2) and 2(3), which is statistically significant. In case of brown marine algae Colpomenia sinuosa was found to be active (titre 2(3)), while Dictyota dichotoma, D. indica and Iyengaria stellata, furnished week titre value as 2(2). The red marine algae screened were 17, out of these 4 spp. showed significant activity (titre 2(3)), and these are Gelidium usmanghani, Gracilaria foliifera Hypnea pannosa and Hynea valentiae. While Scinaia fascicularis, Scinaia indica and Champia parvula were found to be weak in their onset on human erythrocytes. The results obtained were quite in agreement with those reported in the literature. PMID:16414751

  13. Pharmaceutically active secondary metabolites of marine actinobacteria.

    PubMed

    Manivasagan, Panchanathan; Venkatesan, Jayachandran; Sivakumar, Kannan; Kim, Se-Kwon

    2014-04-01

    Marine actinobacteria are one of the most efficient groups of secondary metabolite producers and are very important from an industrial point of view. Many representatives of the order Actinomycetales are prolific producers of thousands of biologically active secondary metabolites. Actinobacteria from terrestrial sources have been studied and screened since the 1950s, for many important antibiotics, anticancer, antitumor and immunosuppressive agents. However, frequent rediscovery of the same compounds from the terrestrial actinobacteria has made them less attractive for screening programs in the recent years. At the same time, actinobacteria isolated from the marine environment have currently received considerable attention due to the structural diversity and unique biological activities of their secondary metabolites. They are efficient producers of new secondary metabolites that show a range of biological activities including antibacterial, antifungal, anticancer, antitumor, cytotoxic, cytostatic, anti-inflammatory, anti-parasitic, anti-malaria, antiviral, antioxidant, anti-angiogenesis, etc. In this review, an evaluation is made on the current status of research on marine actinobacteria yielding pharmaceutically active secondary metabolites. Bioactive compounds from marine actinobacteria possess distinct chemical structures that may form the basis for synthesis of new drugs that could be used to combat resistant pathogens. With the increasing advancement in science and technology, there would be a greater demand for new bioactive compounds synthesized by actinobacteria from various marine sources in future.

  14. Marine Natural Meroterpenes: Synthesis and Antiproliferative Activity

    PubMed Central

    Simon-Levert, Annabel; Menniti, Christophe; Soulère, Laurent; Genevière, Anne-Marie; Barthomeuf, Chantal; Banaigs, Bernard; Witczak, Anne

    2010-01-01

    Meroterpenes are compounds of mixed biogenesis, isolated from plants, microorganisms and marine invertebrates. We have previously isolated and determined the structure for a series of meroterpenes extracted from the ascidian Aplidium aff. densum. Here, we demonstrate the chemical synthesis of three of them and their derivatives, and evaluate their biological activity on two bacterial strains, on sea urchin eggs, and on cancerous and healthy human cells. PMID:20390109

  15. Application of epithermal neutron activation in multielement analysis of silicate rocks employing both coaxial Ge(Li) and low energy photon detector systems

    USGS Publications Warehouse

    Baedecker, P.A.; Rowe, J.J.; Steinnes, E.

    1977-01-01

    The instrumental activation analysis of silicate rocks using epithermal neutrons has been studied using both high resolution coaxial Ge(Li) detectors and low energy photon detectors, and applied to the determination of 23 elements in eight new U.S.G.S. standard rocks. The analytical use X-ray peaks associated with electron capture or internal conversion processes has been evaluated. Of 28 elements which can be considered to be determinable by instrumental means, the epithermal activation approach is capable of giving improved sensitivity and precision in 16 cases, over the normal INAA procedure. In eleven cases the use of the low energy photon detector is thought to show advantages over convertional coaxial Ge(Li) spectroscopy. ?? 1977 Akade??miai Kiado??.

  16. 78 FR 37209 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XC564 Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to Marine Seismic Survey in the Beaufort Sea,...

  17. Marine Biology Activities. Ocean Related Curriculum Activities.

    ERIC Educational Resources Information Center

    Pauls, John

    The ocean affects all of our lives. Therefore, awareness of and information about the interconnections between humans and oceans are prerequisites to making sound decisions for the future. Project ORCA (Ocean Related Curriculum Activities) has developed interdisciplinary curriculum materials designed to meet the needs of students and teachers…

  18. Active source electromagnetic methods for marine munitions

    NASA Astrophysics Data System (ADS)

    Schultz, Gregory; Shubiditze, Fridon; Miller, Jonathan; Evans, Rob

    2011-06-01

    The detection of munitions targets obscured in coastal and marine settings has motivated the need for advanced geophysical technologies suited for underwater deployment. Building on conventional marine electromagnetic theory and based on the use of existing electric and magnetic field sensing designs, we analyze the electromagnetic fields emitted from excited targets in the frequency range between 1 kHz and 1 MHz. We present evidence that employing electromagnetic modes that are higher in frequency relative to those typically used in ground-based sensing yields greater range and sensitivity for underwater surveys. We develop potential design strategies for implementing both magnetic (B) and electric (E) field sources and sensors in the marine environment, and determine optimal arrangements for a potential combined E- and B-field sensing system. The implementation of both 1D analytical and 3D numerical simulations yields the primary and secondary field distributions in representative underwater settings for various sourcereceiver arrangements. We study the electromagnetic field distributions from both electric (voltage-fed dipole) and magnetic field (encased and submerged induction coil) active sources. Application of these concepts provide unique and useful information about targets from the addition of electric field sensing alone as well as through the combination of electric and magnetic field sensing.

  19. Effects of essential oil supplementation of a low-energy diet on performance, intestinal morphology and microflora, immune properties and antioxidant activities in weaned pigs.

    PubMed

    Zeng, Zhikai; Xu, Xiao; Zhang, Qiang; Li, Ping; Zhao, Panfeng; Li, Qingyun; Liu, Jundi; Piao, XiangShu

    2015-03-01

    A total of 144 weaned piglets were used to evaluate the effects of essential oil (EO) supplementation of a low-energy diet on performance, apparent nutrient digestibility, small intestinal morphology, intestinal microflora, immune properties and antioxidant activities in weaned pigs. Pigs received a low-energy diet (negative control, NC, digestible energy = 3250 kcal/kg), NC plus 0.025% EO or a positive control diet (PC, digestible energy = 3400 kcal/kg) for 28 days. Growth performance was similar between the EO group and PC group. However, EO supplementation increased (P < 0.05) average daily gain and the apparent digestibility of dry matter, crude protein and energy compared with pigs fed the NC diet. Greater (P < 0.05) villus height and lower (P < 0.05) counts of Escherichia coli and total anaerobes in the rectum in the EO group were observed compared with NC or PC groups. Pigs fed EO diet had higher (P < 0.05) concentrations of albumin, immunoglobulin A (IgA), IgG and total antioxidant capacity and lower fecal score than pigs fed the PC and NC diets. Above all, this study indicates that supplementation of EO to a low-energy pig diet has beneficial results and obtains similar performance compared with normal energy (PC) diet.

  20. Low Energy Supersymmetry Phenomenology

    SciTech Connect

    Feng, J.

    2003-06-16

    We summarize the current status and future prospects for low energy (weak scale) supersymmetry. In particular, we evaluate the capabilities of various e{sup +}e{sup -}, p{bar p} and pp colliders to discover evidence for supersymmetric particles. Furthermore, assuming supersymmetry is discovered, we discuss capabilities of future facilities to dis-entangle the anticipated spectrum of super-particles, and, via precision measurements, to test mass and coupling parameters for comparison with various theoretical expectations. We comment upon the complementarity of proposed hadron and e{sup +}e{sup -} machines for a comprehensive study of low energy supersymmetry.

  1. Low energy supersymmetry phenomenology

    SciTech Connect

    Baer, H.; Chen, C.H.; Bartl, A.; Feng, J.; Fujii, K.; Gunion, J.; Kamon, T.; Lopez, J.L.; Kao, C.

    1995-04-01

    The authors summarize the current status and future prospects for low energy (weak scale) supersymmetry. In particular, they evaluate the capabilities of various e{sup +}e{sup {minus}}, p{bar p} and pp colliders to discover evidence for supersymmetric particles. Furthermore, assuming supersymmetry is discovered, they discuss capabilities of future facilities to disentangle the anticipated spectrum of super-particles, and, via precision measurements, to test mass and coupling parameters for comparison with various theoretical expectations. The authors then comment upon the complementarity of proposed hadron and e{sup +}e{sup {minus}} machines for a comprehensive study of low energy supersymmetry.

  2. Low energy supersymmetry phenomenology

    SciTech Connect

    Baer, H.; Chen, C.H.; Bartl, A.

    1995-03-01

    The authors summarize the current status and future prospects for low energy (weak scale) supersymmetry. In particular, they evaluate the capabilities of various e{sup +}e{sup {minus}}, p{anti p} and pp colliders to discover evidence for supersymmetric particles. Furthermore, assuming supersymmetry is discovered, they discuss capabilities of future facilities to disentangle the anticipated spectrum of superparticles, and, via precision measurements, to test mass and coupling parameters for comparison with various theoretical expectations. They comment upon the complementarity of proposed hadron and e{sup +}e{sup {minus}} machines for a comprehensive study of low energy supersymmetry.

  3. Maximising the sensitivity of a γ spectrometer for low-energy, low-activity radionuclides using Monte Carlo simulations.

    PubMed

    Britton, R; Burnett, J L; Davies, A V; Regan, P H

    2014-08-01

    Monte-Carlo simulations have been utilised to determine the optimum material and thickness for a γ spectrometer to be used for the assay of radionuclides that emit radiation in the 50-300 keV energy range. Both HPGe and LaBr3(Ce) materials were initially considered for use, however the additional background radiation and lack of resolution in the latter drove the selection of HPGe for further optimisation. Multiple thicknesses were considered for the HPGe detector, with the aim of improving the sensitivity of the system by maximising the efficiency for low energy emissions, and reducing the probability of interaction with (and therefore the continuum from) higher energy photons. The minimum amount of material needed to achieve this was found to be 15 mm for a source that is dominated by high energy (>2.614 MeV) photons, and 20-30 mm for a typical reference source (with photons of energy 59.54 keV-2.614 MeV).

  4. LOW ENERGY COUNTING CHAMBERS

    DOEpatents

    Hayes, P.M.

    1960-02-16

    A beta particle counter adapted to use an end window made of polyethylene terephthalate was designed. The extreme thinness of the film results in a correspondingly high transmission of incident low-energy beta particles by the window. As a consequence, the counting efficiency of the present counter is over 40% greater than counters using conventional mica end windows.

  5. Antioxidant Activity of Hawaiian Marine Algae

    PubMed Central

    Kelman, Dovi; Posner, Ellen Kromkowski; McDermid, Karla J.; Tabandera, Nicole K.; Wright, Patrick R.; Wright, Anthony D.

    2012-01-01

    Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that could be a potential natural source of such antioxidants. The total antioxidant activity of organic extracts of 37 algal samples, comprising of 30 species of Hawaiian algae from 27 different genera was determined. The activity was determined by employing the FRAP (Ferric Reducing Antioxidant Power) assays. Of the algae tested, the extract of Turbinaria ornata was found to be the most active. Bioassay-guided fractionation of this extract led to the isolation of a variety of different carotenoids as the active principles. The major bioactive antioxidant compound was identified as the carotenoid fucoxanthin. These results show, for the first time, that numerous Hawaiian algae exhibit significant antioxidant activity, a property that could lead to their application in one of many useful healthcare or related products as well as in chemoprevention of a variety of diseases including cancer. PMID:22412808

  6. The Low Energy Neutrino Factory

    SciTech Connect

    Bross, Alan; Geer, Steve; Ellis, Malcolm; Fernandez Martinez, Enrique; Li, Tracey; Pascoli, Silvia; Mena, Olga

    2010-03-30

    We show that a low energy neutrino factory with a baseline of 1300 km and muon energy of 4.5 GeV has an excellent physics reach. The results of our optimisation studies demonstrate that such a setup can have remarkable sensitivity to theta{sub 13} and delta for sin{sup 2}(2theta{sub 13})>10{sup -4}, and to the mass hierarchy for sin{sup 2}(2theta{sub 13})>10{sup -3}. We also illustrate the power of the unique combination of golden and platinum channels accessible to the low energy neutrino factory. We have considered both a 20 kton totally active scintillating detector and a 100 kton liquid argon detector as possible detector technologies, finding that a liquid argon detector with very good background rejection can produce sensitivity to theta{sub 13} and delta with that of the International Design Study neutrino factory.

  7. The low energy signaling network

    PubMed Central

    Tomé, Filipa; Nägele, Thomas; Adamo, Mattia; Garg, Abhroop; Marco-llorca, Carles; Nukarinen, Ella; Pedrotti, Lorenzo; Peviani, Alessia; Simeunovic, Andrea; Tatkiewicz, Anna; Tomar, Monika; Gamm, Magdalena

    2014-01-01

    Stress impacts negatively on plant growth and crop productivity, caicultural production worldwide. Throughout their life, plants are often confronted with multiple types of stress that affect overall cellular energy status and activate energy-saving responses. The resulting low energy syndrome (LES) includes transcriptional, translational, and metabolic reprogramming and is essential for stress adaptation. The conserved kinases sucrose-non-fermenting-1-related protein kinase-1 (SnRK1) and target of rapamycin (TOR) play central roles in the regulation of LES in response to stress conditions, affecting cellular processes and leading to growth arrest and metabolic reprogramming. We review the current understanding of how TOR and SnRK1 are involved in regulating the response of plants to low energy conditions. The central role in the regulation of cellular processes, the reprogramming of metabolism, and the phenotypic consequences of these two kinases will be discussed in light of current knowledge and potential future developments. PMID:25101105

  8. The low energy signaling network.

    PubMed

    Tomé, Filipa; Nägele, Thomas; Adamo, Mattia; Garg, Abhroop; Marco-Llorca, Carles; Nukarinen, Ella; Pedrotti, Lorenzo; Peviani, Alessia; Simeunovic, Andrea; Tatkiewicz, Anna; Tomar, Monika; Gamm, Magdalena

    2014-01-01

    Stress impacts negatively on plant growth and crop productivity, caicultural production worldwide. Throughout their life, plants are often confronted with multiple types of stress that affect overall cellular energy status and activate energy-saving responses. The resulting low energy syndrome (LES) includes transcriptional, translational, and metabolic reprogramming and is essential for stress adaptation. The conserved kinases sucrose-non-fermenting-1-related protein kinase-1 (SnRK1) and target of rapamycin (TOR) play central roles in the regulation of LES in response to stress conditions, affecting cellular processes and leading to growth arrest and metabolic reprogramming. We review the current understanding of how TOR and SnRK1 are involved in regulating the response of plants to low energy conditions. The central role in the regulation of cellular processes, the reprogramming of metabolism, and the phenotypic consequences of these two kinases will be discussed in light of current knowledge and potential future developments.

  9. The Antiviral Activities and Mechanisms of Marine Polysaccharides: An Overview

    PubMed Central

    Wang, Wei; Wang, Shi-Xin; Guan, Hua-Shi

    2012-01-01

    Recently, the studies on the antiviral activities of marine natural products, especially marine polysaccharides, are attracting more and more attention all over the world. Marine-derived polysaccharides and their lower molecular weight oligosaccharide derivatives have been shown to possess a variety of antiviral activities. This paper will review the recent progress in research on the antiviral activities and the mechanisms of these polysaccharides obtained from marine organisms. In particular, it will provide an update on the antiviral actions of the sulfated polysaccharides derived from marine algae including carrageenans, alginates, and fucans, relating to their structure features and the structure–activity relationships. In addition, the recent findings on the different mechanisms of antiviral actions of marine polysaccharides and their potential for therapeutic application will also be summarized in detail. PMID:23235364

  10. Bromophenols from marine algae with potential anti-diabetic activities

    NASA Astrophysics Data System (ADS)

    Lin, Xiukun; Liu, Ming

    2012-12-01

    Marine algae contain various bromophenols with a variety of biological activities, including antimicrobial, anticancer, and anti-diabetic effects. Here, we briefly review the recent progress in researches on the biomaterials from marine algae, emphasizing the relationship between the structure and the potential anti-diabetic applications. Bromophenols from marine algae display their hyperglycemic effects by inhibiting the activities of protein tyrosine phosphatase 1B, α-glucosidase, as well as other mechanisms.

  11. Marine Peptides and Their Anti-Infective Activities

    PubMed Central

    Kang, Hee Kyoung; Seo, Chang Ho; Park, Yoonkyung

    2015-01-01

    Marine bioresources are a valuable source of bioactive compounds with industrial and nutraceutical potential. Numerous clinical trials evaluating novel chemotherapeutic agents derived from marine sources have revealed novel mechanisms of action. Recently, marine-derived bioactive peptides have attracted attention owing to their numerous beneficial effects. Moreover, several studies have reported that marine peptides exhibit various anti-infective activities, such as antimicrobial, antifungal, antimalarial, antiprotozoal, anti-tuberculosis, and antiviral activities. In the last several decades, studies of marine plants, animals, and microbes have revealed tremendous number of structurally diverse and bioactive secondary metabolites. However, the treatments available for many infectious diseases caused by bacteria, fungi, and viruses are limited. Thus, the identification of novel antimicrobial peptides should be continued, and all possible strategies should be explored. In this review, we will present the structures and anti-infective activity of peptides isolated from marine sources (sponges, algae, bacteria, fungi and fish) from 2006 to the present. PMID:25603351

  12. 75 FR 80773 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-23

    ... Incidental to Specified Activities; Taking Marine Mammals Incidental to Space Vehicle and Missile Launch... to launching space launch vehicles, long-range ballistic target missiles, and other smaller missile... small numbers of marine mammals incidental to launching space launch vehicles, long-range...

  13. Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings.

    PubMed

    Andersen, Thomas E; Palarasah, Yaseelan; Skjødt, Mikkel-Ole; Ogaki, Ryosuke; Benter, Maike; Alei, Mojagan; Kolmos, Hans J; Koch, Claus; Kingshott, Peter

    2011-07-01

    In the current study we investigate the activation of blood complement on medical device silicone rubber and present a plasma polymerized vinyl pyrrolidone (ppVP) coating which strongly decreases surface-activation of the blood complement system. We show that uncoated silicone and polystyrene are both potent activators of the complement system, measured both as activated, deposited C3b and quantifying fluid-phase release of the cleavage fragment C3c. The ppVP coated silicone exhibits approximately 90% reduced complement activation compared to untreated silicone. Quartz crystal microbalance with dissipation (QCM-D) measurements show relatively strong adsorption of blood proteins including native C3 to the ppVP surface, indicating that reduction of complement activation on ppVP is neither a result of low protein adsorption nor lower direct C3-binding, and is therefore possibly a consequence of differences in the adsorbed protein layer composition. The alternative and classical complement pathways are barely detectable on ppVP while the lectin pathway through MBL/ficolin-2 deposition remains active on ppVP suggesting this pathway is responsible for the remaining subtle activation on the ppVP coated surface. The ppVP surface is furthermore characterized physically and chemically using scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR), which indicates preservation of chemical functionality by the applied plasma process. Overall, the ppVP coating shows a potential for increasing complement-compatibility of blood-contacting devices.

  14. A new experiment to investigate the origin of optical activity using a low energy positron beam of controlled helicity. [molecular biology

    NASA Technical Reports Server (NTRS)

    Gidley, D. W.; Rich, A.; Van House, J. C.; Zitzewitz, P. W.

    1981-01-01

    Previous experiments undertaken in search of a correlation between the origin of optical activity in biological molecules and the helicity of beta particles emitted in nuclear beta decay have not provided any useful results. A description is presented of an experiment in which a low energy polarized positron beam of controlled helicity interacts with an optically active material to form positronium in vacuum. Advantages of the current study compared to the previous experiments are mainly related to a much greater sensitivity. Initially, it will be possible to detect a helicity-dependent asymmetry in triplet positronium formation of 1 part in 10,000. Improvements to better than 1 part in 100,000 should be attainable.

  15. Effective dopant activation by susceptor-assisted microwave annealing of low energy boron implanted and phosphorus implanted silicon

    SciTech Connect

    Zhao, Zhao; Vemuri, Rajitha N. P.; Alford, T. L.; David Theodore, N.; Lu, Wei; Lau, S. S.; Lanz, A.

    2013-12-28

    Rapid processing and reduced end-of-range diffusion result from susceptor-assisted microwave (MW) annealing, making this technique an efficient processing alternative for electrically activating dopants within ion-implanted semiconductors. Sheet resistance and Hall measurements provide evidence of electrical activation. Susceptor-assisted MW annealing, of ion-implanted Si, enables more effective dopant activation and at lower temperatures than required for rapid thermal annealing (RTA). Raman spectroscopy and ion channeling analyses are used to monitor the extent of ion implantation damage and recrystallization. The presence and behavior of extended defects are monitored by cross-section transmission electron microscopy. Phosphorus implanted Si samples experience effective electrical activation upon MW annealing. On the other hand, when boron implanted Si is MW annealed, the growth of extended defects results in reduced crystalline quality that hinders the electrical activation process. Further comparison of dopant diffusion resulting from MW annealing and rapid thermal annealing is performed using secondary ion mass spectroscopy. MW annealed ion implanted samples show less end-of-range diffusion when compared to RTA samples. In particular, MW annealed P{sup +} implanted samples achieve no visible diffusion and equivalent electrical activation at a lower temperature and with a shorter time-duration of annealing compared to RTA. In this study, the peak temperature attained during annealing does not depend on the dopant species or dose, for susceptor-assisted MW annealing of ion-implanted Si.

  16. Effective dopant activation by susceptor-assisted microwave annealing of low energy boron implanted and phosphorus implanted silicon

    NASA Astrophysics Data System (ADS)

    Zhao, Zhao; David Theodore, N.; Vemuri, Rajitha N. P.; Lu, Wei; Lau, S. S.; Lanz, A.; Alford, T. L.

    2013-12-01

    Rapid processing and reduced end-of-range diffusion result from susceptor-assisted microwave (MW) annealing, making this technique an efficient processing alternative for electrically activating dopants within ion-implanted semiconductors. Sheet resistance and Hall measurements provide evidence of electrical activation. Susceptor-assisted MW annealing, of ion-implanted Si, enables more effective dopant activation and at lower temperatures than required for rapid thermal annealing (RTA). Raman spectroscopy and ion channeling analyses are used to monitor the extent of ion implantation damage and recrystallization. The presence and behavior of extended defects are monitored by cross-section transmission electron microscopy. Phosphorus implanted Si samples experience effective electrical activation upon MW annealing. On the other hand, when boron implanted Si is MW annealed, the growth of extended defects results in reduced crystalline quality that hinders the electrical activation process. Further comparison of dopant diffusion resulting from MW annealing and rapid thermal annealing is performed using secondary ion mass spectroscopy. MW annealed ion implanted samples show less end-of-range diffusion when compared to RTA samples. In particular, MW annealed P+ implanted samples achieve no visible diffusion and equivalent electrical activation at a lower temperature and with a shorter time-duration of annealing compared to RTA. In this study, the peak temperature attained during annealing does not depend on the dopant species or dose, for susceptor-assisted MW annealing of ion-implanted Si.

  17. Low-energy neutrinos

    NASA Astrophysics Data System (ADS)

    Ludhova, Livia

    2016-05-01

    There exist several kinds of sources emitting neutrinos in the MeV energy range. These low-energy neutrinos from different sources can be often detected by the same multipurpose detectors. The status-of-art of the field of solar neutrinos, geoneutrinos, and the search for sterile neutrino with artificial neutrino sources is provided here; other neutrino sources, as for example reactor or high-energy neutrinos, are described elsewhere. For each of these three fields, the present-day motivation and open questions, as well as the latest experimental results and future perspectives are discussed.

  18. 78 FR 77433 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-23

    ... one elephant seal has been observed at Pigeon Point. California Sea Lion California sea lions are not... to PISCO to take marine mammals incidental to these same proposed activities (77 FR 72327, December...

  19. Marine Activity Dynamics (M.A.D.). Unit S.

    ERIC Educational Resources Information Center

    Rhode Island State Dept. of Education, Providence. Education Information Center.

    This curriculum guide describes an activity-oriented marine study program, designed for use with middle school children (grade 5). The content focuses primarily upon the life sciences, with some emphasis on chemistry and geology. Following the development of a rationale for the inclusion of marine sciences in the school curriculum, a middle…

  20. Inferences on active faults at the Southern Alps-Liguria basin junction from accurate analysis of low energy seismicity

    NASA Astrophysics Data System (ADS)

    Turino, Chiara; Scafidi, Davide; Eva, Elena; Solarino, Stefano

    2009-10-01

    Seismotectonic studies concern themselves with understanding the distribution of earthquakes in space, time, size and style. Therefore, the better these parameters are known, the most correct the association of any seismic event with the faulting structure that caused it will result. The use of accurate location methods is especially required when dealing with very complex areas, where several faulting systems or relatively small seismogenic structures exist. In fact, even though routinely determined epicentres are capable of revealing the rough picture of the seismicity, they are not suitable for studies of the fine structure of the causative fault, as their location uncertainties are often larger than the source dimension itself. In this work the probabilistic approach of the "Non Linear Localization" has been used to compute precise locations for earthquakes occurred in the last twenty years nearby the Saorge-Taggia line, a complex fault system situated in Western Liguria, close to the border between Italy and France. Together with the Breil-Sospel-Monaco and the Peille-Laghet faults, this line is responsible for the seismic activity of the area. The seismotectonic study is completed through a local tomographic study and the analysis of the focal mechanisms computed for an enlarged area. The results show that the seismicity associated with this fault system is confined within the first 10 km depth. Many clusters of seismic events are identified along the Saorge-Taggia line. The existence of a not previously mapped branch perpendicular to the Saorge-Taggia line is also recognized. Although its position may suggest it to be the continuation of the Breil-Sospel-Monaco fault system towards NE, our finding would rather suggest no association with the fault. The overall results confirm the complexity of the area; in particular the hypothesis that the Saorge-Taggia system may represent the eastward limit of a subalpine crustal block comprised within the Nice Arc, the

  1. Tumoricidal activity of low-energy 160-KV versus 6-MV X-rays against platinum-sensitized F98 glioma cells.

    PubMed

    Lim, Sara N; Pradhan, Anil K; Barth, Rolf F; Nahar, Sultana N; Nakkula, Robin J; Yang, Weilian; Palmer, Alycia M; Turro, Claudia; Weldon, Michael; Bell, Erica Hlavin; Mo, Xiaokui

    2015-01-01

    The purposes of this study were (i) to investigate the differences in effects between 160-kV low-energy and 6-MV high-energy X-rays, both by computational analysis and in vitro studies; (ii) to determine the effects of each on platinum-sensitized F98 rat glioma and murine B16 melanoma cells; and (iii) to describe the in vitro cytotoxicity and in vivo toxicity of a Pt(II) terpyridine platinum (Typ-Pt) complex. Simulations were performed using the Monte Carlo code Geant4 to determine enhancement in absorption of low- versus high-energy X-rays by Pt and to determine dose enhancement factors (DEFs) for a Pt-sensitized tumor phantom. In vitro studies were carried out using Typ-Pt and again with carboplatin due to the unexpected in vivo toxicity of Typ-Pt. Cell survival was determined using clonogenic assays. In agreement with computations and simulations, in vitro data showed up to one log unit reduction in surviving fractions (SFs) of cells treated with 1-4 µg/ml of Typ-Pt and irradiated with 160-kV versus 6-MV X-rays. DEFs showed radiosensitization in the 50-200 keV range, which fell to approximate unity at higher energies, suggesting marginal interactions at MeV energies. Cells sensitized with 1-5 or 7 µg/ml of carboplatin and then irradiated also showed a significant decrease (P < 0.05) in SFs. However, it was unlikely this was due to increased interactions. Theoretical and in vitro studies presented here demonstrated that the tumoricidal activity of low-energy X-rays was greater than that of high-energy X-rays against Pt-sensitized tumor cells. Determining whether radiosensitization is a function of increased interactions will require additional studies. PMID:25266332

  2. Tumoricidal activity of low-energy 160-KV versus 6-MV X-rays against platinum-sensitized F98 glioma cells.

    PubMed

    Lim, Sara N; Pradhan, Anil K; Barth, Rolf F; Nahar, Sultana N; Nakkula, Robin J; Yang, Weilian; Palmer, Alycia M; Turro, Claudia; Weldon, Michael; Bell, Erica Hlavin; Mo, Xiaokui

    2015-01-01

    The purposes of this study were (i) to investigate the differences in effects between 160-kV low-energy and 6-MV high-energy X-rays, both by computational analysis and in vitro studies; (ii) to determine the effects of each on platinum-sensitized F98 rat glioma and murine B16 melanoma cells; and (iii) to describe the in vitro cytotoxicity and in vivo toxicity of a Pt(II) terpyridine platinum (Typ-Pt) complex. Simulations were performed using the Monte Carlo code Geant4 to determine enhancement in absorption of low- versus high-energy X-rays by Pt and to determine dose enhancement factors (DEFs) for a Pt-sensitized tumor phantom. In vitro studies were carried out using Typ-Pt and again with carboplatin due to the unexpected in vivo toxicity of Typ-Pt. Cell survival was determined using clonogenic assays. In agreement with computations and simulations, in vitro data showed up to one log unit reduction in surviving fractions (SFs) of cells treated with 1-4 µg/ml of Typ-Pt and irradiated with 160-kV versus 6-MV X-rays. DEFs showed radiosensitization in the 50-200 keV range, which fell to approximate unity at higher energies, suggesting marginal interactions at MeV energies. Cells sensitized with 1-5 or 7 µg/ml of carboplatin and then irradiated also showed a significant decrease (P < 0.05) in SFs. However, it was unlikely this was due to increased interactions. Theoretical and in vitro studies presented here demonstrated that the tumoricidal activity of low-energy X-rays was greater than that of high-energy X-rays against Pt-sensitized tumor cells. Determining whether radiosensitization is a function of increased interactions will require additional studies.

  3. Low-energy electron interaction with retusin extracted from Maackia amurensis: towards a molecular mechanism of the biological activity of flavonoids.

    PubMed

    Pshenichnyuk, Stanislav A; Elkin, Yury N; Kulesh, Nadezda I; Lazneva, Eleonora F; Komolov, Alexei S

    2015-07-14

    The antioxidant isoflavone retusin efficiently attaches low-energy electrons in vacuo, generating fragment species via dissociative electron attachment (DEA), as has been shown by DEA spectroscopy. According to in silico results obtained by means of density functional theory, retusin is able to attach solvated electrons and could be decomposed under reductive conditions in vivo, for instance, near the mitochondrial electron transport chain, analogous to gas-phase DEA. The most intense decay channels of retusin temporary negative ions were found to be associated with the elimination of H atoms and H2 molecules. Doubly dehydrogenated fragment anions were predicted to possess a quinone structure. It is thought that molecular hydrogen, known for its selective antioxidant properties, can be efficiently generated via electron attachment to retusin in mitochondria and may be responsible for its antioxidant activity. The second abundant species, i.e., quinone bearing an excess negative charge, can serve as an electron carrier and can return the captured electron back to the respiration cycle. The number of OH substituents and their relative positions are crucial for the present molecular mechanism, which can explain the radical scavenging activity of polyphenolic compounds.

  4. A study of neutron radiation quality with a tissue-equivalent proportional counter for a low-energy accelerator-based in vivo neutron activation facility.

    PubMed

    Aslam; Waker, A J

    2011-02-01

    The accelerator-based in vivo neutron activation facility at McMaster University has been used successfully for the measurement of several minor and trace elements in human hand bones due to their importance to health. Most of these in vivo measurements have been conducted at a proton beam energy (E(p)) of 2.00 MeV to optimise the activation of the selected element of interest with an effective dose of the same order as that received in chest X rays. However, measurement of other elements at the same facility requires beam energies other than 2.00 MeV. The range of energy of neutrons produced at these proton beam energies comes under the region where tissue-equivalent proportional counters (TEPCs) are known to experience difficulty in assessing the quality factor and dose equivalent. In this study, the response of TEPCs was investigated to determine the quality factor of neutron fields generated via the (7)Li(p, n)(7)Be reaction as a function of E(p) in the range 1.884-2.56 MeV at the position of hand irradiation in the facility. An interesting trend has been observed in the quality factor based on ICRP 60, Q(ICRP60), such that the maximum value was observed at E(p)=1.884 MeV (E(n)=33±16 keV) and then continued to decline with increasing E(p) until achieving a minimum value at E(p)=2.0 MeV despite a continuous increase in the mean neutron energy with E(p). This observation is contrary to what has been observed with direct fast neutrons where the quality factor was found to increase continuously with an increase in E(p) (i.e. increasing E(n)). The series of measurements conducted with thermal and fast neutron fields demonstrate that the (14)N(n, p)(14)C produced 580 keV protons in the detector play an important role in the response of the counter under 2.0 MeV proton energy (E(n) ≤ 250 keV). In contrast to the lower response of TEPCs to low-energy neutrons, the quality factor is overestimated in the range 1-2 depending on beam energy <2.0 MeV. This study provides

  5. 76 FR 43267 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental To...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ... USAF's activities are considered military readiness activities. Pursuant to the Marine Mammal... geographical region'' provisions and amended the definition of ``harassment'' as it applies to a ``military... Notice of Proposed IHA and request for 30-day public comment published on January 23, 2006 (71 FR...

  6. Incorporation of low energy activated nitrogen onto HOPG surface: Chemical states and thermal stability studies by in-situ XPS and Raman spectroscopy

    NASA Astrophysics Data System (ADS)

    Chandran, Maneesh; Shasha, Michal; Michaelson, Shaul; Hoffman, Alon

    2016-09-01

    In this paper we report the chemical states analysis of activated nitrogen incorporated highly oriented pyrolytic graphite (HOPG) surface under well-controlled conditions. Nitrogen incorporation is carried out by two different processes: an indirect RF nitrogen plasma and low energy (1 keV) N2+ implantation. Bonding configuration, concentration and thermal stability of the incorporated nitrogen species by aforesaid processes are systematically compared by in-situ X-ray photoelectron spectroscopy (XPS). Relatively large concentration of nitrogen is incorporated onto RF nitride HOPG surface (16.2 at.%), compared to N2+ implanted HOPG surface (7.7 at.%). The evolution of N 1s components (N1, N2, N3) with annealing temperature is comprehensively discussed, which indicates that the formation and reorganization of local chemical bonding states are determined by the process of nitridation and not by the prior chemical conditioning (i.e., amorphization or hydrogenation) of the HOPG surface. A combined XPS and Raman spectroscopy studies revealed that N2+ implantation process resulted in a high level of defects to the HOPG surface, which cannot be annealed-out by heat treatment up to 1000 °C. On the other hand, the RF nitrogen plasma process did not produce a high level of surface defects, while incorporating nearly the same amount of stable nitrogen species.

  7. 77 FR 65059 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-24

    ... FR 49922). That notice described, in detail, ION's proposed activity, the marine mammal species that... Register notice for the proposed IHA (77 FR 49922; August 17, 2012), ION would start its seismic survey... proposed IHA (77 FR 49922; August 17, 2012), ION's in-ice seismic survey would be performed in a...

  8. 78 FR 18965 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... on OCS leases in the Chukchi Sea, Alaska, during the 2014 open-water season (78 FR 12542). NMFS... whether to issue an IHA. NMFS refers the reader to the February 22, 2013, Federal Register notice (78 FR... Specified Activities; Taking Marine Mammals Incidental to an Exploration Drilling Program in the Chukchi...

  9. 78 FR 24731 - Taking Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to an...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-26

    ... Proposed IHA, initiating a 30- day public comment period, on February 22, 2013 (78 FR 12542). We then published a notice extending the comment period by 45 days on March 28, 2013 (78 FR 18965). On April 22... Specified Activities; Taking Marine Mammals Incidental to an Exploration Drilling Program in the Chukchi...

  10. 76 FR 4093 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-24

    ... comment periods in the Federal Register on April 19, 2010 (74 FR 20482) for the Beaufort Sea request and May 7, 2010 (74 FR 25730) for the Chukchi Sea request. On May 27, 2010, following the April 20, 2010... Specified Activities; Taking Marine Mammals Incidental to Exploration Drilling Programs in the Chukchi...

  11. 76 FR 33705 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... Register (76 FR 18167) disclosing the effects on marine mammals, making preliminary determinations and... for the proposed IHA (76 FR 18167, April 1, 2011). The activities to be conducted have not changed... reader should refer to the proposed IHA notice (76 FR 18167, April 1, 2011), the IHA application...

  12. 75 FR 49709 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-13

    ..., 2010 (75 FR 27708). That notice described, in detail, Shell's proposed activity, the marine mammal... application, the notice of proposed IHA (75 FR 27708; May 18, 2010) and this document), NMFS determined that... the proposed IHA (75 FR 27708; May 18, 2010), the EA for the issuance of IHAs to Shell and Statoil...

  13. 77 FR 27720 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... notice in the Federal Register (76 FR 58473) discussing the effects on marine mammals and making... the proposed IHA (76 FR 58473, September 21, 2011). The activities to be conducted have not changed... should refer to the proposed IHA notice (76 FR 58473, September 21, 2011), the IHA application...

  14. 78 FR 47495 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-05

    ... Register on May 14, 2012 (78 FR 28412). That notice described, in detail, Shell's proposed activity, the... serious injury (60 FR 28381, May 31, 1995). A private citizen further states that the marine survey is... limited to only those that may involve non-serious injury (60 FR 28379; May 31, 1995). While the...

  15. 75 FR 49759 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-13

    ... FR 32379). That notice described, in detail, Statoil's proposed activity, the marine mammal species... application, the notice of proposed IHA (75 FR 32379; June 8, 2010) and this document), NMFS determined that... in the proposed IHA (75 FR 32379; June 18, 2010), the EA for the issuance of IHAs to Shell...

  16. 77 FR 40007 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-06

    ... of 3D seismic images requires the deployment of many parallel cables spaced close together over the... on May 1, 2012 (77 FR 25830). That notice described, in detail, BP's proposed activity, the marine... that the language is consistent with that referenced in the main body of the Federal Register...

  17. Intense low energy positron beams

    SciTech Connect

    Lynn, K.G.; Jacobsen, F.M.

    1993-12-31

    Intense positron beams are under development or being considered at several laboratories. Already today a few accelerator based high intensity, low brightness e{sup +} beams exist producing of the order of 10{sup 8} {minus} 10{sup 9} e{sup +}/sec. Several laboratories are aiming at high intensity, high brightness e{sup +} beams with intensities greater than 10{sup 9} e{sup +}/sec and current densities of the order of 10{sup 13} {minus} 10{sup 14} e{sup +} sec{sup {minus}} {sup 1}cm{sup {minus}2}. Intense e{sup +} beams can be realized in two ways (or in a combination thereof) either through a development of more efficient B{sup +} moderators or by increasing the available activity of B{sup +} particles. In this review we shall mainly concentrate on the latter approach. In atomic physics the main trust for these developments is to be able to measure differential and high energy cross-sections in e{sup +} collisions with atoms and molecules. Within solid state physics high intensity, high brightness e{sup +} beams are in demand in areas such as the re-emission e{sup +} microscope, two dimensional angular correlation of annihilation radiation, low energy e{sup +} diffraction and other fields. Intense e{sup +} beams are also important for the development of positronium beams, as well as exotic experiments such as Bose condensation and Ps liquid studies.

  18. Biological Activity of Recently Discovered Halogenated Marine Natural Products

    PubMed Central

    Gribble, Gordon W.

    2015-01-01

    This review presents the biological activity—antibacterial, antifungal, anti-parasitic, antiviral, antitumor, antiinflammatory, antioxidant, and enzymatic activity—of halogenated marine natural products discovered in the past five years. Newly discovered examples that do not report biological activity are not included. PMID:26133553

  19. Sustainable production of biologically active molecules of marine based origin.

    PubMed

    Murray, Patrick M; Moane, Siobhan; Collins, Catherine; Beletskaya, Tanya; Thomas, Olivier P; Duarte, Alysson W F; Nobre, Fernando S; Owoyemi, Ifeloju O; Pagnocca, Fernando C; Sette, L D; McHugh, Edward; Causse, Eric; Pérez-López, Paula; Feijoo, Gumersindo; Moreira, Ma T; Rubiolo, Juan; Leirós, Marta; Botana, Luis M; Pinteus, Susete; Alves, Celso; Horta, André; Pedrosa, Rui; Jeffryes, Clayton; Agathos, Spiros N; Allewaert, Celine; Verween, Annick; Vyverman, Wim; Laptev, Ivan; Sineoky, Sergei; Bisio, Angela; Manconi, Renata; Ledda, Fabio; Marchi, Mario; Pronzato, Roberto; Walsh, Daniel J

    2013-09-25

    The marine environment offers both economic and scientific potential which are relatively untapped from a biotechnological point of view. These environments whilst harsh are ironically fragile and dependent on a harmonious life form balance. Exploitation of natural resources by exhaustive wild harvesting has obvious negative environmental consequences. From a European industry perspective marine organisms are a largely underutilised resource. This is not due to lack of interest but due to a lack of choice the industry faces for cost competitive, sustainable and environmentally conscientious product alternatives. Knowledge of the biotechnological potential of marine organisms together with the development of sustainable systems for their cultivation, processing and utilisation are essential. In 2010, the European Commission recognised this need and funded a collaborative RTD/SME project under the Framework 7-Knowledge Based Bio-Economy (KBBE) Theme 2 Programme 'Sustainable culture of marine microorganisms, algae and/or invertebrates for high value added products'. The scope of that project entitled 'Sustainable Production of Biologically Active Molecules of Marine Based Origin' (BAMMBO) is outlined. Although the Union is a global leader in many technologies, it faces increasing competition from traditional rivals and emerging economies alike and must therefore improve its innovation performance. For this reason innovation is placed at the heart of a European Horizon 2020 Strategy wherein the challenge is to connect economic performance to eco performance. This article provides a synopsis of the research activities of the BAMMBO project as they fit within the wider scope of sustainable environmentally conscientious marine resource exploitation for high-value biomolecules.

  20. Ocean Planet. Interdisciplinary Marine Science Activities.

    ERIC Educational Resources Information Center

    Branca, Barbara

    The Ocean Planet is a traveling exhibition from the Smithsonian Institution designed to share with the public what recent research has revealed about the oceans and to encourage ocean conservation. This booklet of lessons and activities adapts several themes from the exhibition for use in middle and high school classrooms. Lesson plans include:…

  1. A low energy electron magnetometer

    NASA Technical Reports Server (NTRS)

    Singh, J. J.; Wood, G. M., Jr.; Rayborn, G. H.; White, F. A.

    1979-01-01

    The concept of a highly sensitive magnetometer based on the deflection of low energy electron beams in magnetic fields is analyzed. Because of its extremely low mass and consequently high e/m ratio, a low energy electron is easily deflected in a magnetic field, thus providing a basis for very low field measurement. Calculations for a specific instrument design indicate that a low energy electron magnetometer (LEEM) can measure magnetic fields as low as 1000 nT. The anticipated performance of LEEM is compared with that of the existing high resolution magnetometers in selected applications. The fast response time of LEEM makes it especially attractive as a potential instrument for magnetic signature analysis in large engineering systems.

  2. Antioxidant Activity of Marine Algal Polyphenolic Compounds: A Mechanistic Approach.

    PubMed

    Fernando, I P Shanura; Kim, Misook; Son, Kwang-Tae; Jeong, Yoonhwa; Jeon, You-Jin

    2016-07-01

    Polyphenolic compounds isolated from marine algae exhibit a broad spectrum of beneficial biological properties, including antioxidant, anticancer, antimicrobial, anti-inflammatory, and antidiabetic activities, along with several other bioactivities centered on their antioxidant properties. Consequently, polyphenolic compounds are increasingly being investigated for their potential use in food, cosmetic, and pharmaceutical applications. The antioxidant activities of these compounds have been explored widely through experimental studies. Nonetheless, a theoretical understanding of the structural and electronic properties could broaden research perspectives, leading to the identification and synthesis of efficient structural analogs with prophylactic uses. This review briefly summarizes the current state of knowledge regarding antioxidant polyphenolic compounds in marine algae with an attempt to describe the structure-activity relationship. PMID:27332715

  3. Low Energy Schools in Ireland

    ERIC Educational Resources Information Center

    Heffernan, Martin

    2004-01-01

    Out of a commitment to reducing carbon dioxide emissions, Ireland's Department of Education and Science has designed and constructed two low energy schools, in Tullamore, County Offaly, and Raheen, County Laois. With energy use in buildings responsible for approximately 55% of the CO[subscript 2] released into the atmosphere and a major…

  4. Volcanic tremor at Volcán de Colima, México recorded during May 2002 and its interactions with the seismic signals produced by low-energy explosive activity and rockfalls

    NASA Astrophysics Data System (ADS)

    Zobin, Vyacheslav M.; Reyes, Gabriel A.; Bretón, Mauricio

    2016-05-01

    The May 2002 eruption episode at Volcán de Colima, México represented the transition period between two stages of effusive activity which were characterized by the formation of lava flows. The short-period seismic signals, recorded during May 2002 at a distance of 1.6 km from the crater, were represented by volcanic tremor and the signals produced by low-energy explosions and rockfalls. Two types of volcanic tremor were recognized: harmonic with two fundamental spectral frequencies of 1.2-1.4 Hz and 1.6-1.7 Hz and non-harmonic. The existence of two fundamental frequencies of volcanic tremor may indicate a two-vent magmatic conduit. No clear relationship between the variations in the spectral content of tremor and occurrence of explosions was observed. The waveforms of the signals, produced by low-energy explosions and rockfalls and recorded on the background of volcanic tremor, were strongly modulated by the low-frequency harmonic tremor signals, forming, in this manner, pseudo-long period events. Fourier analysis of the seismic signals associated with low-energy explosions and rockfalls but recorded on the background of regular seismic noise indicated their high-frequency sources characterized by dominant frequencies within 2-3 Hz and 3-4.5 Hz, respectively.

  5. Human activities change marine ecosystems by altering predation risk.

    PubMed

    Madin, Elizabeth M P; Dill, Lawrence M; Ridlon, April D; Heithaus, Michael R; Warner, Robert R

    2016-01-01

    In ocean ecosystems, many of the changes in predation risk - both increases and decreases - are human-induced. These changes are occurring at scales ranging from global to local and across variable temporal scales. Indirect, risk-based effects of human activity are known to be important in structuring some terrestrial ecosystems, but these impacts have largely been neglected in oceans. Here, we synthesize existing literature and data to explore multiple lines of evidence that collectively suggest diverse human activities are changing marine ecosystems, including carbon storage capacity, in myriad ways by altering predation risk. We provide novel, compelling evidence that at least one key human activity, overfishing, can lead to distinct, cascading risk effects in natural ecosystems whose magnitude exceeds that of presumed lethal effects and may account for previously unexplained findings. We further discuss the conservation implications of human-caused indirect risk effects. Finally, we provide a predictive framework for when human alterations of risk in oceans should lead to cascading effects and outline a prospectus for future research. Given the speed and extent with which human activities are altering marine risk landscapes, it is crucial that conservation and management policy considers the indirect effects of these activities in order to increase the likelihood of success and avoid unfortunate surprises. PMID:26448058

  6. Human activities change marine ecosystems by altering predation risk.

    PubMed

    Madin, Elizabeth M P; Dill, Lawrence M; Ridlon, April D; Heithaus, Michael R; Warner, Robert R

    2016-01-01

    In ocean ecosystems, many of the changes in predation risk - both increases and decreases - are human-induced. These changes are occurring at scales ranging from global to local and across variable temporal scales. Indirect, risk-based effects of human activity are known to be important in structuring some terrestrial ecosystems, but these impacts have largely been neglected in oceans. Here, we synthesize existing literature and data to explore multiple lines of evidence that collectively suggest diverse human activities are changing marine ecosystems, including carbon storage capacity, in myriad ways by altering predation risk. We provide novel, compelling evidence that at least one key human activity, overfishing, can lead to distinct, cascading risk effects in natural ecosystems whose magnitude exceeds that of presumed lethal effects and may account for previously unexplained findings. We further discuss the conservation implications of human-caused indirect risk effects. Finally, we provide a predictive framework for when human alterations of risk in oceans should lead to cascading effects and outline a prospectus for future research. Given the speed and extent with which human activities are altering marine risk landscapes, it is crucial that conservation and management policy considers the indirect effects of these activities in order to increase the likelihood of success and avoid unfortunate surprises.

  7. Signal Transducers and Activators of Transcription (STAT) Regulatory Networks in Marine Organisms: From Physiological Observations towards Marine Drug Discovery.

    PubMed

    Lee, Jin-Young; Orlikova, Barbora; Diederich, Marc

    2015-08-07

    Part of our ocean's richness comes from its extensive history of supporting life, resulting in a highly diverse ecological system. To date, over 250,000 species of marine organisms have been identified, but it is speculated that the actual number of marine species exceeds one million, including several hundreds of millions of species of marine microorganisms. Past studies suggest that approximately 70% of all deep-sea microorganisms, gorgonians, and sea sponges produce secondary metabolites with anti-cancer activities. Recently, novel FDA-approved drugs derived from marine sponges have been shown to reduce metastatic breast cancer, malignant lymphoma, and Hodgkin's disease. Despite the fact that many marine natural products have been shown to possess a good inhibition potential against most of the cancer-related cell signaling pathways, only a few marine natural products have been shown to target JAK/STAT signaling. In the present paper, we describe the JAK/STAT signaling pathways found in marine organisms, before elaborating on the recent advances in the field of STAT inhibition by marine natural products and the potential application in anti-cancer drug discovery.

  8. Signal Transducers and Activators of Transcription (STAT) Regulatory Networks in Marine Organisms: From Physiological Observations towards Marine Drug Discovery

    PubMed Central

    Lee, Jin-Young; Orlikova, Barbora; Diederich, Marc

    2015-01-01

    Part of our ocean’s richness comes from its extensive history of supporting life, resulting in a highly diverse ecological system. To date, over 250,000 species of marine organisms have been identified, but it is speculated that the actual number of marine species exceeds one million, including several hundreds of millions of species of marine microorganisms. Past studies suggest that approximately 70% of all deep-sea microorganisms, gorgonians, and sea sponges produce secondary metabolites with anti-cancer activities. Recently, novel FDA-approved drugs derived from marine sponges have been shown to reduce metastatic breast cancer, malignant lymphoma, and Hodgkin’s disease. Despite the fact that many marine natural products have been shown to possess a good inhibition potential against most of the cancer-related cell signaling pathways, only a few marine natural products have been shown to target JAK/STAT signaling. In the present paper, we describe the JAK/STAT signaling pathways found in marine organisms, before elaborating on the recent advances in the field of STAT inhibition by marine natural products and the potential application in anti-cancer drug discovery. PMID:26262624

  9. 77 FR 49921 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-17

    ...NMFS received an application from ION Geophysical (ION) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment only, incidental to a proposed marine seismic survey in the Beaufort and Chukchi Seas, Alaska, between October and December 2012. Pursuant to the Marine Mammal Protection Act (MMPA), NMFS is requesting comments on its proposal to issue an IHA to ION to......

  10. Microbial activity in the marine deep biosphere: progress and prospects.

    PubMed

    Orcutt, Beth N; Larowe, Douglas E; Biddle, Jennifer F; Colwell, Frederick S; Glazer, Brian T; Reese, Brandi Kiel; Kirkpatrick, John B; Lapham, Laura L; Mills, Heath J; Sylvan, Jason B; Wankel, Scott D; Wheat, C Geoff

    2013-01-01

    The vast marine deep biosphere consists of microbial habitats within sediment, pore waters, upper basaltic crust and the fluids that circulate throughout it. A wide range of temperature, pressure, pH, and electron donor and acceptor conditions exists-all of which can combine to affect carbon and nutrient cycling and result in gradients on spatial scales ranging from millimeters to kilometers. Diverse and mostly uncharacterized microorganisms live in these habitats, and potentially play a role in mediating global scale biogeochemical processes. Quantifying the rates at which microbial activity in the subsurface occurs is a challenging endeavor, yet developing an understanding of these rates is essential to determine the impact of subsurface life on Earth's global biogeochemical cycles, and for understanding how microorganisms in these "extreme" environments survive (or even thrive). Here, we synthesize recent advances and discoveries pertaining to microbial activity in the marine deep subsurface, and we highlight topics about which there is still little understanding and suggest potential paths forward to address them. This publication is the result of a workshop held in August 2012 by the NSF-funded Center for Dark Energy Biosphere Investigations (C-DEBI) "theme team" on microbial activity (www.darkenergybiosphere.org).

  11. Microbial activity in the marine deep biosphere: progress and prospects

    PubMed Central

    Orcutt, Beth N.; LaRowe, Douglas E.; Biddle, Jennifer F.; Colwell, Frederick S.; Glazer, Brian T.; Reese, Brandi Kiel; Kirkpatrick, John B.; Lapham, Laura L.; Mills, Heath J.; Sylvan, Jason B.; Wankel, Scott D.; Wheat, C. Geoff

    2013-01-01

    The vast marine deep biosphere consists of microbial habitats within sediment, pore waters, upper basaltic crust and the fluids that circulate throughout it. A wide range of temperature, pressure, pH, and electron donor and acceptor conditions exists—all of which can combine to affect carbon and nutrient cycling and result in gradients on spatial scales ranging from millimeters to kilometers. Diverse and mostly uncharacterized microorganisms live in these habitats, and potentially play a role in mediating global scale biogeochemical processes. Quantifying the rates at which microbial activity in the subsurface occurs is a challenging endeavor, yet developing an understanding of these rates is essential to determine the impact of subsurface life on Earth's global biogeochemical cycles, and for understanding how microorganisms in these “extreme” environments survive (or even thrive). Here, we synthesize recent advances and discoveries pertaining to microbial activity in the marine deep subsurface, and we highlight topics about which there is still little understanding and suggest potential paths forward to address them. This publication is the result of a workshop held in August 2012 by the NSF-funded Center for Dark Energy Biosphere Investigations (C-DEBI) “theme team” on microbial activity (www.darkenergybiosphere.org). PMID:23874326

  12. 75 FR 67951 - Takes of Marine Mammals Incidental to Specified Activities; Piling and Structure Removal in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-04

    ... respect to the ecology and life history of potentially affected marine mammals (e.g., will harassment... National Oceanic and Atmospheric Administration Takes of Marine Mammals Incidental to Specified Activities... implementing the Marine Mammal Protection Act (MMPA), as amended, notification is hereby given that...

  13. 75 FR 80471 - Takes of Marine Mammals Incidental to Specified Activities; St. George Reef Light Station...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-22

    ...), for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment incidental to... incidentally harass, by Level B harassment only, four species of marine mammals during the specified activity... intentional, taking by harassment of small numbers of marine mammals of a species or population stock,...

  14. 33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... this part. (b) Only for this part, the boundary between Activities Far East and Activities Europe... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Activities Far East Marine... ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone....

  15. 33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... this part. (b) Only for this part, the boundary between Activities Far East and Activities Europe... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Activities Far East Marine... ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone....

  16. 33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... this part. (b) Only for this part, the boundary between Activities Far East and Activities Europe... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Activities Far East Marine... ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone....

  17. 33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... this part. (b) Only for this part, the boundary between Activities Far East and Activities Europe... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Activities Far East Marine... ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone....

  18. 33 CFR 3.70-20 - Activities Far East Marine Inspection Zone.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... this part. (b) Only for this part, the boundary between Activities Far East and Activities Europe... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Activities Far East Marine... ZONES Fourteenth Coast Guard District § 3.70-20 Activities Far East Marine Inspection Zone....

  19. Marine Omega-3 Phospholipids: Metabolism and Biological Activities

    PubMed Central

    Burri, Lena; Hoem, Nils; Banni, Sebastiano; Berge, Kjetil

    2012-01-01

    The biological activities of omega-3 fatty acids (n-3 FAs) have been under extensive study for several decades. However, not much attention has been paid to differences of dietary forms, such as triglycerides (TGs) versus ethyl esters or phospholipids (PLs). New innovative marine raw materials, like krill and fish by-products, present n-3 FAs mainly in the PL form. With their increasing availability, new evidence has emerged on n-3 PL biological activities and differences to n-3 TGs. In this review, we describe the recently discovered nutritional properties of n-3 PLs on different parameters of metabolic syndrome and highlight their different metabolic bioavailability in comparison to other dietary forms of n-3 FAs. PMID:23203133

  20. Low-Energy Proton Testing Methodology

    NASA Technical Reports Server (NTRS)

    Pellish, Jonathan A.; Marshall, Paul W.; Heidel, David F.; Schwank, James R.; Shaneyfelt, Marty R.; Xapsos, M.A.; Ladbury, Raymond L.; LaBel, Kenneth A.; Berg, Melanie; Kim, Hak S.; Phan, Anthony; Friendlich, M.R.; Rodbell, Kenneth P.; Hakey, Mark C.; Dodd, Paul E.; Reed, Robert A.; Weller, Robert A.; Mendenhall, Marcus H.; Sierawski, B.D.

    2009-01-01

    Use of low-energy protons and high-energy light ions is becoming necessary to investigate current-generation SEU thresholds. Systematic errors can dominate measurements made with low-energy protons. Range and energy straggling contribute to systematic error. Low-energy proton testing is not a step-and-repeat process. Low-energy protons and high-energy light ions can be used to measure SEU cross section of single sensitive features; important for simulation.

  1. Low-Energy Cosmic Rays

    NASA Astrophysics Data System (ADS)

    Wiedenbeck, M. E.; ACE/CRIS Collaboration

    2002-12-01

    Cosmic rays with energies below about 10 GeV/nucleon have been measured with high precision as a result of experiments on the HEAO, Ulysses, and ACE spacecrafts. The observations provide energy spectra, elemental abundances, and isotopic composition for elements up through Z=30. They include both stable and radioactive nuclides that are synthesized in stars or are produced by nuclear fragmentation during diffusion at high energies through interstellar medium. From these data one obtains a rather detailed picture of the origin of low-energy cosmic rays. For refractory species, the cosmic-ray source composition closely resembles that of the Sun, suggesting that cosmic rays are accelerated from a well-mixed sample of interstellar matter. A chemical fractionation process has depleted the abundances of volatile elements relative to refractories. Using various radioactive clock isotopes it has been shown that particle acceleration occurs at least 105 years after supernova nucleosynthesis and that the accelerated particles diffuse in the Galaxy for approximately 15 Myr after acceleration. Energy spectra and secondary-to-primary ratios are reasonably well accounted for by models in which particles gain the bulk of their energy in a single encounter with a strong shock. Among the large number of species that have been measured, 22Ne stands out as the only nuclide with an abundance that is clearly much different than solar. To test models proposed to account for this anomaly, the data are being analyzed for predicted smaller effects on abundances of other nuclides. In addition to providing a detailed understanding of the origin and acceleration of low-energy cosmic rays, these data are providing constraints on the chemical evolution of interstellar matter. This work was supported by NASA at Caltech (under grant NAG5-6912), JPL, NASA/GSFC, and Washington U.

  2. Distribution and significance of heterotrophic marine bacteria with antibacterial activity.

    PubMed Central

    Nair, S; Simidu, U

    1987-01-01

    Bacteria with antibacterial activity were isolated from seawater, sediments, phytoplankton, and zooplankton of Suruga, Sagami, and Tokyo Bays and from soft corals and sponges collected from the Taiwan coast. Of the 726 strains isolated, 37 showed antibacterial activity against either Vibrio parahaemolyticus (ATCC 17802) or Staphylococcus aureus (P209). Sediment harbored the lowest number of these forms of bacteria, and those from Tokyo Bay did not show any activity. Attached isolates showed greater activity compared with free-living forms. Relatively high numbers of strains with antibacterial activity were associated with phytoplankton. Among the zooplankton isolates, cladocerans harbored the maximum number of antibacterial strains. Isolates were more inhibitory to gram-positive test cultures. Autoinhibition was observed only among 8% of the isolates. Marine nonproducers were more susceptible. Pseudomonas/Alteromonas species made up 81.0% of isolates, of which 30% were pigmented strains. The absence or reduction in number of bacteria with antibacterial activity in Tokyo Bay is attributed to its eutrophic nature, which may tend to moderate the production of antibacterial compounds. PMID:3435149

  3. 75 FR 25729 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ...NMFS received an application from Shell Offshore Inc. (Shell) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to offshore exploration drilling on Outer Continental Shelf (OCS) leases in the Chukchi Sea, Alaska. Pursuant to the Marine Mammal Protection Act (MMPA), NMFS is requesting comments on its proposal to issue an IHA to Shell to take, by......

  4. 78 FR 12541 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-22

    ...NMFS received an application from ConocoPhillips Company (COP) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to offshore exploration drilling on Outer Continental Shelf (OCS) leases in the Chukchi Sea, Alaska. Pursuant to the Marine Mammal Protection Act (MMPA), NMFS is requesting comments on its proposal to issue an IHA to COP to take, by......

  5. 77 FR 27321 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-09

    ... of an incidental harassment authorization. SUMMARY: In accordance with the Marine Mammal Protection Act (MMPA) regulations, notification is hereby given that NMFS has issued an Incidental Harassment Authorization (IHA) to Shell Gulf of Mexico Inc. (Shell) to take marine mammals, by harassment, incidental...

  6. 77 FR 58255 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey off the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-19

    ... National Environmental Policy Act, 42 U.S.C. 4321 et seq. Marine Seismic Survey in the Pacific Ocean off Central California, 2012'' (EA). NSF's EA incorporates a draft ``Environmental Assessment of Marine... (Lissodelphis borealis). waters. California/Oregon/ Washington stock. Risso's dolphin (Grampus Deep water,...

  7. AMP as a low-energy charge signal autonomously initiates assembly of AXIN-AMPK-LKB1 complex for AMPK activation.

    PubMed

    Zhang, Ya-Lin; Guo, Huiling; Zhang, Chen-Song; Lin, Shu-Yong; Yin, Zhenyu; Peng, Yongying; Luo, Hui; Shi, Yuzhe; Lian, Guili; Zhang, Cixiong; Li, Mengqi; Ye, Zhiyun; Ye, Jing; Han, Jiahuai; Li, Peng; Wu, Jia-Wei; Lin, Sheng-Cai

    2013-10-01

    The AMP-activated protein kinase (AMPK) is a master regulator of metabolic homeostasis by sensing cellular energy status. AMPK is mainly activated via phosphorylation by LKB1 when cellular AMP/ADP levels are increased. However, how AMP/ADP brings about AMPK phosphorylation remains unclear. Here, we show that it is AMP, but not ADP, that drives AXIN to directly tether LKB1 to phosphorylate AMPK. The complex formation of AXIN-AMPK-LKB1 is greatly enhanced in glucose-starved or AICAR-treated cells and in cell-free systems supplemented with exogenous AMP. Depletion of AXIN abrogated starvation-induced AMPK-LKB1 colocalization. Importantly, adenovirus-based knockdown of AXIN in the mouse liver impaired AMPK activation and caused exacerbated fatty liver after starvation, underscoring an essential role of AXIN in AMPK activation. These findings demonstrate an initiating role of AMP and demonstrate that AXIN directly transmits AMP binding of AMPK to its activation by LKB1, uncovering the mechanistic route for AMP to elicit AMPK activation by LKB1.

  8. Diversity of Secondary Metabolites from Marine Bacillus Species: Chemistry and Biological Activity

    PubMed Central

    Mondol, Muhammad Abdul Mojid; Shin, Hee Jae; Islam, Mohammad Tofazzal

    2013-01-01

    Marine Bacillus species produce versatile secondary metabolites including lipopeptides, polypeptides, macrolactones, fatty acids, polyketides, and isocoumarins. These structurally diverse compounds exhibit a wide range of biological activities, such as antimicrobial, anticancer, and antialgal activities. Some marine Bacillus strains can detoxify heavy metals through reduction processes and have the ability to produce carotenoids. The present article reviews the chemistry and biological activities of secondary metabolites from marine isolates. Side by side, the potential for application of these novel natural products from marine Bacillus strains as drugs, pesticides, carotenoids, and tools for the bioremediation of heavy metal toxicity are also discussed. PMID:23941823

  9. Low energy neutrinos in Super-Kamiokande

    NASA Astrophysics Data System (ADS)

    Sekiya, Hiroyuki

    2016-05-01

    Super-Kamiokande (SK), a 50 kton water Cherenkov detector, observes 8B solar neutrinos via neutrino-electron elastic scattering. The analysis threshold was successfully lowered to 3.5 MeV (recoil electron kinetic energy) in SK-IV. To date SK has observed solar neutrinos for 18 years. An analysis regarding possible correlations between the solar neutrino flux and the 11 year solar activity cycle is shown. With large statistics, SK searches for distortions of the solar neutrino energy spectrum caused by the MSW resonance in the core of the sun. SK also searches for a day/night solar neutrino flux asymmetry induced by the matter in the Earth. The Super-Kamiokande Gd (SK-Gd) project is the upgrade of the SK detector via the addition of water-soluble gadolinium (Gd) salt. This modification will enable it to efficiently identify low energy anti-neutrinos. SK-Gd will pursue low energy physics currently inaccessible to SK due to backgrounds. The most important will be the world’s first observation of the diffuse supernova neutrino background. The main R&D program towards SK-Gd is EG ADS: a 200 ton, fully instrumented tank built in a new cavern in the Kamioka mine.

  10. Navy and Marine Corps active duty mortality patterns for 1995 to 1999.

    PubMed

    Almond, Myron D; Carlton, Jan; Bohnker, Bruce K

    2003-01-01

    The authors analyze all Navy and Marine Corps active duty deaths from January 1, 1995 through December 31, 1999 (Navy, N=1231; Marine Corps, N=701). Data were obtained from official Navy and Marine Corps sources, including the Report of Casualty (DD form 1300) and the Navy Personnel Casualty Report (Control Symbol NMPC 1770-4) or the Marine Corps Personnel Casualty Report (MC-3040-02), as appropriate. Overall fatality rates were 68.2 per 100,000 active duty Navy personnel and 84.2 for active duty Marine Corps personnel. Rates were generally lower than those noted in previous studies and lower than comparable civilian groups. The officer fatality rates were strongly affected by aircraft mishap-related deaths. The only subgroup displaying higher rates than their civilian counterparts was mishap-related deaths for enlisted Marines age 17 to 24 years old. PMID:12546243

  11. Marine natural products with anti-HIV activities in the last decade.

    PubMed

    Zhou, Xuefeng; Liu, Juan; Yang, Bin; Lin, Xiuping; Yang, Xian-Wen; Liu, Yonghong

    2013-01-01

    Marine organisms have been proven to be excellent sources of biologically active compounds against HIV. This review gives an overview of 132 natural products from marine sources obtained during the last decade (2002-2011), which exhibit anti-HIV activity toward different biological targets. Sponges contribute more than half of all anti-HIV natural products from marine organisms, mainly as alkaloids and cyclic depsipeptides. In addition, some macromolecules are considered as potential anti-HIV agents, including lectins from algae and marine invertebrates, as well as sulfated polysaccharides from algae. In the reviewed marine natural products, many active ingredients act as HIV entry inhibitors, one class of new anti-HIV agents, and may be regarded as potential candidates for the development of novel anti-HIV agents. The other features of development in the marine original anti-HIV natural products in this ten years are also discussed.

  12. Resonance Raman scattering of butadiene: Vibronic activity of a bu mode demonstrates the presence of a 1Ag symmetry excited electronic state at low energy

    NASA Astrophysics Data System (ADS)

    Chadwick, Richard R.; Zgierski, Marek Z.; Hudson, Bruce S.

    1991-11-01

    Resonance Raman spectra of buta-1,3-diene-d0 and buta-1,3-diene-1,1,4,4-d4 have been obtained with ultraviolet excitation from 239.5 to 199.9 nm. Activity of the first overtone of mode 24, the bu symmetry CCC chain deformation mode, is observed with excitation energy below the origin of the 1 1Bu state. This vibronic activity of a nontotally symmetric mode is shown to be evidence of resonance with the 2 1Ag state of butadiene. A quantitative analysis of the ratio of intensities of 2ν24 to ν9, the ag symmetry CCC chain deformation mode, demonstrates that enhancement of 2ν24 cannot be due to resonance with the 1 1Bu state. The resonance enhancement behavior of this overtone band also shows that it is of vibronic origin rather than Franck-Condon allowed. The intensity pattern seen for the modes of bu symmetry is fully consistent with the results of a quantitative calculation of vibronic activity for the eight bu symmetry modes. The 2 1Ag electronic state is estimated to be ca. 0.25 eV below the 1 1Bu electronic state. Overtones of out-of-plane C-H bending and CH2 twisting modes are seen with excitation radiation near the peak of the transition to the 2 1Ag state, indicating that the 2 1Ag state of butadiene has appreciably lower resistance to deformation along out-of-plane coordinates than does the ground electronic state. This is consistent with the expectations of semiempirical calculations.

  13. 78 FR 8497 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey Off the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ..., 2012, NMFS published a notice in the Federal Register (77 FR 58256) disclosing the effects on marine...: Notice is hereby given that Lamont-Doherty Earth Observatory of Columbia University (L-DEO),...

  14. Low-Energy Sputtering Research

    NASA Technical Reports Server (NTRS)

    Ray, P. K.; Shutthanandan, V.

    1999-01-01

    An experimental study is described to measure low-energy (less than 600 eV) sputtering yields of molybdenum with xenon ions using Rutherford backscattering spectroscopy (RBS) and secondary neutral mass spectroscopy (SNMS). An ion gun was used to generate the ion beam. The ion current density at the target surface was approximately 30 (micro)A/sq cm. For RBS measurements, the sputtered material was collected on a thin aluminum strip which was mounted on a semi-circular collector plate. The target was bombarded with 200 and 500 eV xenon ions at normal incidence. The differential sputtering yields were measured using the RBS method with 1 MeV helium ions. The differential yields were fitted with a cosine fitting function and integrated with respect to the solid angle to provide the total sputtering yields. The sputtering yields obtained using the RBS method are in reasonable agreement with those measured by other researchers using different techniques. For the SNMS measurements, 150 to 600 eV xenon ions were used at 50deg angle of incidence. The SNMS spectra were converted to sputtering yields for perpendicular incidence by normalizing SNMS spectral data at 500 eV with the yield measured by Rutherford backscattering spectrometry. Sputtering yields as well as the shape of the yield-energy curve obtained in this manner are in reasonable agreement with those measured by other researchers using different techniques. Sputtering yields calculated by using two semi-spherical formulations agree reasonably well with measured data. The isotopic composition of secondary ions were measured by bombarding copper with xenon ions at energies ranging from 100 eV to 1.5 keV. The secondary ion flux was found to be enriched in heavy isotopes at low incident ion energies. The heavy isotope enrichment was observed to decrease with increasing impact energy. Beyond 700 eV, light isotopes were sputtered preferentially with the enrichment remaining nearly constant.

  15. Complete-active-space second-order perturbation theory (CASPT2//CASSCF) study of the dissociative electron attachment in canonical DNA nucleobases caused by low-energy electrons (0-3 eV).

    PubMed

    Francés-Monerris, Antonio; Segarra-Martí, Javier; Merchán, Manuela; Roca-Sanjuán, Daniel

    2015-12-01

    Low-energy (0-3 eV) ballistic electrons originated during the irradiation of biological material can interact with DNA/RNA nucleobases yielding transient-anion species which undergo decompositions. Since the discovery that these reactions can eventually lead to strand breaking of the DNA chains, great efforts have been dedicated to their study. The main fragmentation at the 0-3 eV energy range is the ejection of a hydrogen atom from the specific nitrogen positions. In the present study, the methodological approach introduced in a previous work on uracil [I. González-Ramírez et al., J. Chem. Theory Comput. 8, 2769-2776 (2012)] is employed to study the DNA canonical nucleobases fragmentations of N-H bonds induced by low-energy electrons. The approach is based on minimum energy path and linear interpolation of internal coordinates computations along the N-H dissociation channels carried out at the complete-active-space self-consistent field//complete-active-space second-order perturbation theory level. On the basis of the calculated theoretical quantities, new assignations for the adenine and cytosine anion yield curves are provided. In addition, the π1 (-) and π2 (-) states of the pyrimidine nucleobases are expected to produce the temporary anions at electron energies close to 1 and 2 eV, respectively. Finally, the present theoretical results do not allow to discard neither the dipole-bound nor the valence-bound mechanisms in the range of energies explored, suggesting that both possibilities may coexist in the experiments carried out with the isolated nucleobases.

  16. Complete-active-space second-order perturbation theory (CASPT2//CASSCF) study of the dissociative electron attachment in canonical DNA nucleobases caused by low-energy electrons (0-3 eV)

    NASA Astrophysics Data System (ADS)

    Francés-Monerris, Antonio; Segarra-Martí, Javier; Merchán, Manuela; Roca-Sanjuán, Daniel

    2015-12-01

    Low-energy (0-3 eV) ballistic electrons originated during the irradiation of biological material can interact with DNA/RNA nucleobases yielding transient-anion species which undergo decompositions. Since the discovery that these reactions can eventually lead to strand breaking of the DNA chains, great efforts have been dedicated to their study. The main fragmentation at the 0-3 eV energy range is the ejection of a hydrogen atom from the specific nitrogen positions. In the present study, the methodological approach introduced in a previous work on uracil [I. González-Ramírez et al., J. Chem. Theory Comput. 8, 2769-2776 (2012)] is employed to study the DNA canonical nucleobases fragmentations of N-H bonds induced by low-energy electrons. The approach is based on minimum energy path and linear interpolation of internal coordinates computations along the N-H dissociation channels carried out at the complete-active-space self-consistent field//complete-active-space second-order perturbation theory level. On the basis of the calculated theoretical quantities, new assignations for the adenine and cytosine anion yield curves are provided. In addition, the π1- and π2- states of the pyrimidine nucleobases are expected to produce the temporary anions at electron energies close to 1 and 2 eV, respectively. Finally, the present theoretical results do not allow to discard neither the dipole-bound nor the valence-bound mechanisms in the range of energies explored, suggesting that both possibilities may coexist in the experiments carried out with the isolated nucleobases.

  17. Complete-active-space second-order perturbation theory (CASPT2//CASSCF) study of the dissociative electron attachment in canonical DNA nucleobases caused by low-energy electrons (0-3 eV)

    SciTech Connect

    Francés-Monerris, Antonio; Segarra-Martí, Javier; Merchán, Manuela; Roca-Sanjuán, Daniel

    2015-12-07

    Low-energy (0-3 eV) ballistic electrons originated during the irradiation of biological material can interact with DNA/RNA nucleobases yielding transient-anion species which undergo decompositions. Since the discovery that these reactions can eventually lead to strand breaking of the DNA chains, great efforts have been dedicated to their study. The main fragmentation at the 0-3 eV energy range is the ejection of a hydrogen atom from the specific nitrogen positions. In the present study, the methodological approach introduced in a previous work on uracil [I. González-Ramírez et al., J. Chem. Theory Comput. 8, 2769-2776 (2012)] is employed to study the DNA canonical nucleobases fragmentations of N–H bonds induced by low-energy electrons. The approach is based on minimum energy path and linear interpolation of internal coordinates computations along the N–H dissociation channels carried out at the complete-active-space self-consistent field//complete-active-space second-order perturbation theory level. On the basis of the calculated theoretical quantities, new assignations for the adenine and cytosine anion yield curves are provided. In addition, the π{sub 1}{sup −} and π{sub 2}{sup −} states of the pyrimidine nucleobases are expected to produce the temporary anions at electron energies close to 1 and 2 eV, respectively. Finally, the present theoretical results do not allow to discard neither the dipole-bound nor the valence-bound mechanisms in the range of energies explored, suggesting that both possibilities may coexist in the experiments carried out with the isolated nucleobases.

  18. 76 FR 6406 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-04

    ...; proposed incidental harassment authorization; request for comments. SUMMARY: NMFS has received an application from the U.S. Navy (Navy) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to construction activities as part of a pile replacement project....

  19. 76 FR 79409 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-21

    ...; proposed incidental harassment authorization; request for comments. SUMMARY: NMFS has received an application from the U.S. Navy (Navy) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to construction activities as part of a wharf construction...

  20. 77 FR 25408 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-30

    ...; proposed incidental harassment authorization; request for comments. SUMMARY: NMFS has received an application from the U.S. Navy (Navy) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to construction activities as part of a pile replacement project....

  1. 76 FR 4300 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    ...; proposed incidental harassment authorization; request for comments. SUMMARY: NMFS has received an application from the U.S. Navy (Navy) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to pile driving activities as part of a test pile program. Pursuant to...

  2. Towards Low Energy Atrial Defibrillation.

    PubMed

    Walsh, Philip; Kodoth, Vivek; McEneaney, David; Rodrigues, Paola; Velasquez, Jose; Waterman, Niall; Escalona, Omar

    2015-01-01

    transcutaneous power transfer and sensing of ICI during cardioversion are evidenced as key to the advancement of low-energy atrial defibrillation. PMID:26404298

  3. Towards Low Energy Atrial Defibrillation

    PubMed Central

    Walsh, Philip; Kodoth, Vivek; McEneaney, David; Rodrigues, Paola; Velasquez, Jose; Waterman, Niall; Escalona, Omar

    2015-01-01

    transcutaneous power transfer and sensing of ICI during cardioversion are evidenced as key to the advancement of low-energy atrial defibrillation. PMID:26404298

  4. 78 FR 48180 - Consolidation of Officer in Charge, Marine Inspection For Outer Continental Shelf Activities...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-07

    ... SECURITY Coast Guard Consolidation of Officer in Charge, Marine Inspection For Outer Continental Shelf Activities; Eighth Coast Guard District AGENCY: Coast Guard, DHS. ACTION: Notice and request for comments. SUMMARY: The Coast Guard is considering establishing a single Officer in Charge, Marine Inspection...

  5. 76 FR 330 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-04

    ... ringed seal (75 FR 77476) and a notice of proposed threatened and not warranted status for subspecies and distinct population segments of the bearded seal (75 FR 77496) in the Federal Register. Neither species is... supports a diverse assemblage of marine mammals, including: Bowhead, gray, beluga, killer, minke,...

  6. 76 FR 49737 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-11

    ....94 17.00 physalus). water. \\12\\. Blue whale (Balaneoptera Rare Offshore, shelf, 3,500 \\13\\...... EN D... being requested (i.e., North Pacific right and blue whales) are sighted at any distance from the vessel..., NMFS published a notice in the Federal Register (76 FR 33246) discussing the effects on marine...

  7. 75 FR 64996 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-21

    ... 21 species of marine mammals. These species include: Bryde's whale; blue whale; sperm whale; humpback... blue (Balaenoptera musculus) whales. NMFS has presented a more detailed discussion of the status of... whale, blue whale, Mesoplodon spp., rough-toothed, bottlenose, pantropical spotted, spinner,...

  8. 76 FR 20325 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... notice in the Federal Register (76 FR 6430) disclosing the effects on marine mammals, making preliminary... outlined the purpose of the program in a previous notice for the proposed IHA (76 FR 6430, February 4, 2011... vessel and acoustic source specifications, the reader should refer to the proposed IHA notice (76 FR...

  9. 77 FR 6065 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-07

    ... subducting and bending Pacific plate. The objective is to understand the water cycle within subduction-zone... notice in the Federal Register (75 FR 8652) with preliminary determinations and a proposed IHA. Ship... the Federal Register (76 FR 77782) disclosing the effects on marine mammals, making...

  10. 77 FR 51773 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-27

    ... on monitoring of marine mammal reactions to rocket launches at Vandenberg Air Force Base. In those... Administration (NOAA), Commerce. ACTION: Notice; proposed incidental harassment authorization; request for... non-target species, if such an alternative action is chosen, during a proposed house mouse...

  11. 77 FR 73434 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-10

    ... IHA to Apache for their first season of seismic acquisition in Cook Inlet (77 FR 27720). Except for... densities or deeper waters (76 FR 20180, April 11, 2011). Cetaceans Beluga Whales--Cook Inlet beluga whales... acoustic research in Cook Inlet by the Alaska Department of Fish and Game and the National Marine...

  12. 78 FR 12720 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-25

    ... in Cook Inlet (77 FR 27720). On December 10, 2012, NMFS published a notice in the Federal Register (77 FR 73434) discussing the effects on marine mammals and making preliminary determinations regarding.... In the notice of the proposed IHA (77 FR 73434, December 10, 2012), NMFS described the second area...

  13. 77 FR 27283 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-09

    ...) for the onset of Level B harassment from pulsed sound sources. The Notice of Proposed IHA (76 FR 68974... harassment. Bowheads may engage in avoidance behavior preventing their exposure to these levels of sound, and...; issuance of an incidental harassment authorization. SUMMARY: In accordance with the Marine...

  14. 78 FR 28411 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-14

    ... Mexico Inc. (Shell) for an Incidental Harassment Authorization (IHA) to take marine mammals, by... not limited to, migration, breathing, nursing, breeding, feeding, or sheltering . Summary of Request.... For example, at the Guerreo Negro Lagoon in Baja California, Mexico, which is one of the...

  15. Marine Invertebrate Metabolites with Anticancer Activities: Solutions to the "Supply Problem".

    PubMed

    Gomes, Nelson G M; Dasari, Ramesh; Chandra, Sunena; Kiss, Robert; Kornienko, Alexander

    2016-05-01

    Marine invertebrates provide a rich source of metabolites with anticancer activities and several marine-derived agents have been approved for the treatment of cancer. However, the limited supply of promising anticancer metabolites from their natural sources is a major hurdle to their preclinical and clinical development. Thus, the lack of a sustainable large-scale supply has been an important challenge facing chemists and biologists involved in marine-based drug discovery. In the current review we describe the main strategies aimed to overcome the supply problem. These include: marine invertebrate aquaculture, invertebrate and symbiont cell culture, culture-independent strategies, total chemical synthesis, semi-synthesis, and a number of hybrid strategies. We provide examples illustrating the application of these strategies for the supply of marine invertebrate-derived anticancer agents. Finally, we encourage the scientific community to develop scalable methods to obtain selected metabolites, which in the authors' opinion should be pursued due to their most promising anticancer activities.

  16. Anti-Obesity Activity of the Marine Carotenoid Fucoxanthin

    PubMed Central

    Gammone, Maria Alessandra; D’Orazio, Nicolantonio

    2015-01-01

    Nowadays the global tendency towards physical activity reduction and an augmented dietary intake of fats, sugars and calories is leading to a growing propagation of overweight, obesity and lifestyle-related diseases, such diabetes, hypertension, dyslipidemia and metabolic syndrome. In particular, obesity, characterized as a state of low-level inflammation, is a powerful determinant both in the development of insulin resistance and in the progression to type 2 diabetes. A few molecular targets offer hope for anti-obesity therapeutics. One of the keys to success could be the induction of uncoupling protein 1 (UCP1) in abdominal white adipose tissue (WAT) and the regulation of cytokine secretions from both abdominal adipose cells and macrophage cells infiltrated into adipose tissue. Anti-obesity effects of fucoxanthin, a characteristic carotenoid, exactly belonging to xanthophylls, have been reported. Nutrigenomic studies reveal that fucoxanthin induces UCP1 in abdominal WAT mitochondria, leading to the oxidation of fatty acids and heat production in WAT. Fucoxanthin improves insulin resistance and decreases blood glucose levels through the regulation of cytokine secretions from WAT. The key structure of anti-obesity effect is suggested to be the carotenoid end of the polyene chromophore, which contains an allenic bond and two hydroxyl groups. Fucoxanthin, which can be isolated from edible brown seaweeds, recently displayed its many physiological functions and biological properties. We reviewed recent studies and this article aims to explain essential background of fucoxanthin, focusing on its promising potential anti-obesity effects. In this respect, fucoxanthin can be developed into promising marine drugs and nutritional products, in order to become a helpful functional food. PMID:25871295

  17. Bacterial activities driving arsenic speciation and solubility in marine sediments

    NASA Astrophysics Data System (ADS)

    Battaglia-Brunet, F.; Seby, F.; Crouzet, C.; Joulian, C.; Mamindy-Pajany, Y.; Guezennec, A. G.; Hurel, C.; Marmier, N.; Bataillard, P.

    2012-04-01

    Harbour and marina sediments represent particular environments, with high concentrations in organic carbon and pollutants. Over 50 million m3 of marine sediments are dredged every year in French maritime and commercial ports, to maintain the water depth suitable for navigation, and the most part of them is discharged in deeper sea zones. The present study aimed to elucidate, using a range of complementary approaches, the influence of bacterial activity on arsenic speciation and mobility in marina sediments. Two sites were considered: L'Estaque, impacted by metallurgical activities and by the commercial port of Marseille, and St-Mandrier, less polluted, affected by classical chemical pollutants associated to professional and recreational boating. Arsenic concentration was noticeably higher in l'Estaque sediment (200-350 mg/kg) than in St-Mandrier sediment (15-50 mg/kg). In the solid phases, As(III) was the dominant species in L'Estaque sediment, whereas As(V) was the main form in St Mandrier sediment. At both sites, arsenic was the major trace element detected in interstitial water. Free sulfide and thio-arsenic complexes were detected in the interstitial water of l'Estaque sediment, suggesting a role of sulfate-reduction bacterial activity on arsenic solubility. Anaerobic microcosm experiments confirmed this hypothesis, as stimulation of sulfate-reduction induced a dramatic increase of arsenic concentration in the liquid phase, linked to the formation of soluble thio-arsenic complexes. Nevertheless, microcosms performed in aerobic conditions showed that bacterial activity globally decreased the transfer of arsenic from the sediment toward the overlying water. A red-brown fine layer developed at the sediment-water interface. Altogether, these results suggest that the sediment-water interface zone and the close transition area between aerobic and anaerobic conditions host intense biogeochemical reactions involving As, Fe and S species. These reactions most probably

  18. Low energy availability in the marathon and other endurance sports.

    PubMed

    Loucks, Anne B

    2007-01-01

    Energy availability is the amount of dietary energy remaining after exercise training for all other metabolic processes. Excessively low energy availability impairs reproductive and skeletal health, although genetics and age may alter an individual's initial conditions and sensitivity when low energy availability is imposed. Many marathon runners and other endurance athletes reduce energy availability either (i) intentionally to modify body size and composition for improving performance; (ii) compulsively in a psychopathological pattern of disordered eating; or (iii) inadvertently because there is no strong biological drive to match energy intake to activity-induced energy expenditure. Inadvertent low energy availability is more extreme when consuming a low fat, high carbohydrate diet. Low energy availability, reproductive disorders, low bone mineral density and stress fractures are more common in female than male athletes. Functional menstrual disorders caused by low energy availability should be diagnosed by excluding diseases that also disrupt menstrual cycles. To determine energy availability (in units of kilocalories or kilojoules per kilogram of fat-free mass), athletes can record their diets and use diet analysis software to calculate energy intake, measure energy expenditure during exercise using a heart monitor and measure fat-free mass using a bioelectrical impedance body composition scale. All are commercially available at consumer prices.

  19. Techniques and methods for the low-energy neutrino detection

    NASA Astrophysics Data System (ADS)

    Ranucci, Gioacchino

    2016-04-01

    Low-energy neutrino physics and astrophysics has been one of the most active field of particle physics research over the past two decades, achieving important and sometimes unexpected results, which have paved the way for a bright future of further exciting studies. The methods, the techniques and the technologies employed for the construction of the many experiments which acted as important players in this area of investigation have been crucial elements to reach the many accumulated physics successes. The topic covered in this review is, thus, the description of the main features of the set of methodologies at the basis of the design, construction and operation of low-energy neutrino detectors.

  20. Low energy ghosts and the Jeans' instability

    NASA Astrophysics Data System (ADS)

    Gümrükçüoǧlu, A. Emir; Mukohyama, Shinji; Sotiriou, Thomas P.

    2016-09-01

    We show that a massless canonical scalar field minimally coupled to general relativity can become a tachyonic ghost at low energies around a background in which the scalar's gradient is spacelike. By performing a canonical transformation we demonstrate that this low energy ghost can be recast, at the level of the action, in a form of a fluid that undergoes a Jeans-like instability affecting only modes with large wavelength. This illustrates that low energy tachyonic ghosts do not lead to a catastrophic quantum vacuum instability, unlike the usual high-energy ghost degrees of freedom.

  1. Solar-assisted low energy dwellings

    SciTech Connect

    Esbensen, T V

    1980-02-01

    The Zero Energy House Group was formed as a subproject of the CCMS Solar Energy Pilot Study in 1974 by seven participating countries experimenting with solar-assisted low-energy dwellings for temperate and northern European climatic conditions. A Zero Energy House is one in which solar energy is used to meet the reduced energy needs of buildings incorporating various thermal energy conservation features. This final report of the Zero Energy House Group includes brief descriptions of 13 major low-energy dwellings in the participating CCMS countries. An overall assessment of the state-of-the-art in solar-assisted low-energy dwellings is also included.

  2. IONS (ANURADHA): Ionization states of low energy cosmic rays

    NASA Technical Reports Server (NTRS)

    Biswas, S.; Chakraborti, R.; Cowsik, R.; Durgaprasad, N.; Kajarekar, P. J.; Singh, R. K.; Vahia, M. N.; Yadav, J. S.; Dutt, N.; Goswami, J. N.

    1987-01-01

    IONS (ANURADHA), the experimental payload designed specifically to determine the ionization states, flux, composition, energy spectra and arrival directions of low energy (10 to 100 MeV/amu) anomalous cosmic ray ions of helium to iron in near-Earth space, had a highly successful flight and operation Spacelab-3 mission. The experiment combines the accuracy of a highly sensitive CR-39 nuclear track detector with active components included in the payload to achieve the experimental objectives. Post-flight analysis of detector calibration pieces placed within the payload indicated no measurable changes in detector response due to its exposure in spacelab environment. Nuclear tracks produced by alpha-particles, oxygen group and Fe ions in low energy anomalous cosmic rays were identified. It is calculated that the main detector has recorded high quality events of about 10,000 alpha-particles and similar number of oxygen group and heavier ions of low energy cosmic rays.

  3. Low-energy particle radiation environment at synchronous altitude

    NASA Technical Reports Server (NTRS)

    Shelley, E. G.; Lens, S. K.

    1972-01-01

    The degradation of thermal control coatings of satellites due to the effects of low energy charged particles in the space environment is discussed. Data obtained from ATS-5 satellite measurement of proton and electron fluxes are presented. The variations in electron density, proton density, and magnetic activity are presented to show correlations which exist between these space factors.

  4. HEAO-1 analysis of Low Energy Detectors (LED)

    NASA Technical Reports Server (NTRS)

    Nousek, John A.

    1992-01-01

    The activities at Penn State University are described. During the period Oct. 1990 to Dec. 1991 work on HEAO-1 analysis of the Low Energy Detectors (LED) concentrated on using the improved detector spectral simulation model and fitting diffuse x-ray background spectral data. Spectral fitting results, x-ray point sources, and diffuse x-ray sources are described.

  5. Marine Careers.

    ERIC Educational Resources Information Center

    Gordon, Bernard L.

    The five papers in this publication on marine careers were selected so that science teachers, guidance councilors, and students could benefit from the experience and knowledge of individuals active in marine science. The areas considered are indicated by the titles: Professional Careers in Marine Science with the Federal Government, Marine Science…

  6. Secondary Metabolites from the Marine Algal-Derived Endophytic Fungi: Chemical Diversity and Biological Activity.

    PubMed

    Zhang, Peng; Li, Xin; Wang, Bin-Gui

    2016-06-01

    Marine algal-derived endophytic fungi have attracted considerable attention in the most recent two decades due to their prolific production of structurally diverse secondary metabolites with various biological activities. This review summarizes a total of 182 natural products isolated from marine algal-derived endophytic fungi in the past two decades. The emphasis is on the unique chemical diversity of these metabolic products, together with relevant biological activities.

  7. The Science of Low Energy Nuclear Reactions

    NASA Astrophysics Data System (ADS)

    Storms, Edmund

    2007-03-01

    The large literature describing the anomalous behavior attributed to cold fusion or low energy nuclear reactions has been critically described in a recently published book. Over 950 publications are evaluated allowing the phenomenon to be understood. A new class of nuclear reactions has been discovered that are able to generate practical energy without significant radiation or radioactivity. Edmund K Storms, The Science of Low Energy Nuclear Reactions, in press (2006). Also see: http://www.lenr-canr.org/StudentsGuide.htm .

  8. The Crowded Sea: Incorporating Multiple Marine Activities in Conservation Plans Can Significantly Alter Spatial Priorities

    PubMed Central

    Mazor, Tessa; Possingham, Hugh P.; Edelist, Dori; Brokovich, Eran; Kark, Salit

    2014-01-01

    Successful implementation of marine conservation plans is largely inhibited by inadequate consideration of the broader social and economic context within which conservation operates. Marine waters and their biodiversity are shared by a host of stakeholders, such as commercial fishers, recreational users and offshore developers. Hence, to improve implementation success of conservation plans, we must incorporate other marine activities while explicitly examining trade-offs that may be required. In this study, we test how the inclusion of multiple marine activities can shape conservation plans. We used the entire Mediterranean territorial waters of Israel as a case study to compare four planning scenarios with increasing levels of complexity, where additional zones, threats and activities were added (e.g., commercial fisheries, hydrocarbon exploration interests, aquaculture, and shipping lanes). We applied the marine zoning decision support tool Marxan to each planning scenario and tested a) the ability of each scenario to reach biodiversity targets, b) the change in opportunity cost and c) the alteration of spatial conservation priorities. We found that by including increasing numbers of marine activities and zones in the planning process, greater compromises are required to reach conservation objectives. Complex plans with more activities incurred greater opportunity cost and did not reach biodiversity targets as easily as simplified plans with less marine activities. We discovered that including hydrocarbon data in the planning process significantly alters spatial priorities. For the territorial waters of Israel we found that in order to protect at least 10% of the range of 166 marine biodiversity features there would be a loss of ∼15% of annual commercial fishery revenue and ∼5% of prospective hydrocarbon revenue. This case study follows an illustrated framework for adopting a transparent systematic process to balance biodiversity goals and economic

  9. Low-energy electron scattering from cyanamide

    NASA Astrophysics Data System (ADS)

    Wang, Kedong; Guo, Shuangcheng; Meng, Ju; Huang, Xiaotian; Wang, Yongfeng

    2016-09-01

    The low-energy electron collisions with cyanamide molecule are investigated by using the UK molecular R -matrix codes for electron energies ranging from 0.01 eV to 10 eV. Three models including static-exchange, static-exchange plus polarization, and close-coupling (CC) approximations are employed to reveal the dynamic interaction. Elastic (integrated and differential), momentum-transfer, and excitation cross sections from the ground state to the three low-lying electron excited states have been presented. Two shape resonances, two core-excited resonances, and two Feshbach resonances are detected in the CC approximation. The role of active space in the target and scattering problem including the resonances is discussed. The precise resonance parameters are found to be sensitive to the treatment of polarization effects employed. These resonances may be responsible for the fragments observed in a recent experiment of the dissociative electron attachments to cyanamide. Since the cyanamide molecule has a large permanent dipole moment, a Born closure procedure is used to account for the contribution of partial waves higher than l =4 to obtain converged cross sections.

  10. 77 FR 87 - Taking of Marine Mammals Incidental to Specified Activities; U.S. Marine Corps Training Exercises...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... significant potential to injure a marine mammal or marine mammal stock in the wild [Level A Harassment]; or (ii) Any act that disturbs or is likely to disturb a marine mammal or marine mammal stock in the wild... December 1, 2011 (75 FR 72807; November 26, 2010). Weapon delivery training will occur at two BTs:...

  11. The Structure-Activity Relationship between Marine Algae Polysaccharides and Anti-Complement Activity

    PubMed Central

    Jin, Weihua; Zhang, Wenjing; Liang, Hongze; Zhang, Quanbin

    2015-01-01

    In this study, 33 different polysaccharides were prepared to investigate the structure-activity relationships between the polysaccharides, mainly from marine algae, and anti-complement activity in the classical pathway. Factors considered included extraction methods, fractionations, molecular weight, molar ratio of galactose to fucose, sulfate, uronic acid (UA) content, linkage, branching, and the type of monosaccharide. It was shown that the larger the molecular weights, the better the activities. The molar ratio of galactose (Gal) to fucose (Fuc) was a positive factor at a concentration lower than 10 µg/mL, while it had no effect at a concentration more than 10 µg/mL. In addition, sulfate was necessary; however, the sulfate content, the sulfate pattern, linkage and branching had no effect at a concentration of more than 10 µg/mL. Moreover, the type of monosaccharide had no effect. Laminaran and UA fractions had no activity; however, they could reduce the activity by decreasing the effective concentration of the active composition when they were mixed with the active compositions. The effect of the extraction methods could not be determined. Finally, it was observed that sulfated galactofucan showed good anti-complement activity after separation. PMID:26712768

  12. Larvicidal Activity against Aedes aegypti and Molluscicidal Activity against Biomphalaria glabrata of Brazilian Marine Algae

    PubMed Central

    Guedes, Elíca Amara Cecília; de Carvalho, Cenira M.; Ribeiro Junior, Karlos Antonio Lisboa; Lisboa Ribeiro, Thyago Fernando; de Barros, Lurdiana Dayse; de Lima, Maria Raquel Ferreira; Prado Moura, Flávia de Barros; Goulart Sant'Ana, Antônio Euzebio

    2014-01-01

    This study investigated the biological activities of five benthic marine algae collected from Northeastern Region of Brazil. The tested activities included larvicidal activity against Aedes aegypti, molluscicidal activity against Biomphalaria glabrata, and toxicity against Artemia salina. Extracts of Ulva lactuca (Chlorophyta), Padina gymnospora, Sargassum vulgare (Phaeophyta), Hypnea musciformis, and Digenea simplex (Rhodophyta) were prepared using different solvents of increasing polarity, including dichloromethane, methanol, ethanol, and water. Of the extracts screened, the dichloromethane extracts of H. musciformis and P. gymnospora exhibited the highest activities and were subjected to bioassay-guided fractionation in hexane and chloroform. The chloroform fractions of the P. gymnospora and H. musciformis extracts showed molluscicidal activity at values below 40 μg·mL−1 (11.1460 μg·mL−1 and 25.8689 μg·mL−1, resp.), and the chloroform and hexane fractions of P. gymnospora showed larvicidal activity at values below 40 μg·mL−1 (29.018 μg·mL−1 and 17.230 μg·mL−1, resp.). The crude extracts were not toxic to A. salina, whereas the chloroform and hexane fractions of P. gymnospora (788.277 μg·mL−1 and 706.990 μg·mL−1) showed moderate toxicity, indicating that the toxic compounds present in these algae are nonpolar. PMID:24688787

  13. Larvicidal Activity against Aedes aegypti and Molluscicidal Activity against Biomphalaria glabrata of Brazilian Marine Algae.

    PubMed

    Guedes, Elíca Amara Cecília; de Carvalho, Cenira M; Ribeiro Junior, Karlos Antonio Lisboa; Lisboa Ribeiro, Thyago Fernando; de Barros, Lurdiana Dayse; de Lima, Maria Raquel Ferreira; Prado Moura, Flávia de Barros; Goulart Sant'ana, Antônio Euzebio

    2014-01-01

    This study investigated the biological activities of five benthic marine algae collected from Northeastern Region of Brazil. The tested activities included larvicidal activity against Aedes aegypti, molluscicidal activity against Biomphalaria glabrata, and toxicity against Artemia salina. Extracts of Ulva lactuca (Chlorophyta), Padina gymnospora, Sargassum vulgare (Phaeophyta), Hypnea musciformis, and Digenea simplex (Rhodophyta) were prepared using different solvents of increasing polarity, including dichloromethane, methanol, ethanol, and water. Of the extracts screened, the dichloromethane extracts of H. musciformis and P. gymnospora exhibited the highest activities and were subjected to bioassay-guided fractionation in hexane and chloroform. The chloroform fractions of the P. gymnospora and H. musciformis extracts showed molluscicidal activity at values below 40  μ g·mL(-1) (11.1460  μ g·mL(-1) and 25.8689  μ g·mL(-1), resp.), and the chloroform and hexane fractions of P. gymnospora showed larvicidal activity at values below 40  μ g·mL(-1) (29.018  μ g·mL(-1) and 17.230  μ g·mL(-1), resp.). The crude extracts were not toxic to A. salina, whereas the chloroform and hexane fractions of P. gymnospora (788.277  μ g·mL(-1) and 706.990  μ g·mL(-1)) showed moderate toxicity, indicating that the toxic compounds present in these algae are nonpolar. PMID:24688787

  14. Caffeine Use among Active Duty Navy and Marine Corps Personnel

    PubMed Central

    Knapik, Joseph J.; Trone, Daniel W.; McGraw, Susan; Steelman, Ryan A.; Austin, Krista G.; Lieberman, Harris R.

    2016-01-01

    Data from the National Health and Nutrition Examination Survey (NHANES) indicate 89% of Americans regularly consume caffeine, but these data do not include military personnel. This cross-sectional study examined caffeine use in Navy and Marine Corps personnel, including prevalence, amount of daily consumption, and factors associated with use. A random sample of Navy and Marine Corps personnel was contacted and asked to complete a detailed questionnaire describing their use of caffeine-containing substances, in addition to their demographic, military, and lifestyle characteristics. A total of 1708 service members (SMs) completed the questionnaire. Overall, 87% reported using caffeinated beverages ≥1 time/week, with caffeine users consuming a mean ± standard error of 226 ± 5 mg/day (242 ± 7 mg/day for men, 183 ± 8 mg/day for women). The most commonly consumed caffeinated beverages (% users) were coffee (65%), colas (54%), teas (40%), and energy drinks (28%). Multivariable logistic regression modeling indicated that characteristics independently associated with caffeine use (≥1 time/week) included older age, white race/ethnicity, higher alcohol consumption, and participating in less resistance training. Prevalence of caffeine use in these SMs was similar to that reported in civilian investigations, but daily consumption (mg/day) was higher. PMID:27735834

  15. Analysis of marine sediment and lobster hepatopancreas reference materials by instrumental photon activation

    SciTech Connect

    Landsberger, S.; Davidson, W.F.

    1985-01-01

    By use of instrumental photon activation analysis, twelve trace (As, Ba, Cr, Co, Mn, Ni, Pb, Sb, Sr, U, Zn, and Zr) and eight minor (C, Na, Mg, Co, K, Ca, Tl, and Fe) elements were determined in a certified marine sediment standard reference material as well as eight trace (Mn, Ni, Cu, Zn, As, Sr, Cd, and Pb) and four minor (Na, Mg, Cl, and Ca) elements in a certified marine tissue (lobster hepatopancreas) standard reference material. The precision and accuracy of the present results when compared to the accepted values clearly demonstrate the reliability of this nondestructive technique and its applicability to marine environmental or marine geochemical studies. 24 references, 4 figures, 3 tables.

  16. Marine-derived Penicillium in Korea: diversity, enzyme activity, and antifungal properties.

    PubMed

    Park, Myung Soo; Fong, Jonathan J; Oh, Seung-Yoon; Kwon, Kae Kyoung; Sohn, Jae Hak; Lim, Young Woon

    2014-08-01

    The diversity of marine-derived Penicillium from Korea was investigated using morphological and multigene phylogenetic approaches, analyzing sequences of the internal transcribed spacer region, β-tubulin gene, and RNA polymerase subunit II gene. In addition, the biological activity of all isolated strains was evaluated. We tested for the extracellular enzyme activity of alginase, endoglucanase, and β-glucosidase, and antifungal activity against two plant pathogens (Colletotrichum acutatum and Fusarium oxysporum). A total of 184 strains of 36 Penicillium species were isolated, with 27 species being identified. The most common species were Penicillium polonicum (19.6 %), P. rubens (11.4 %), P. chrysogenum (11.4 %), and P. crustosum (10.9 %). The diversity of Penicillium strains isolated from soil (foreshore soil and sand) and marine macroorganisms was higher than the diversity of strains isolated from seawater. While many of the isolated strains showed alginase and β-glucosidase activity, no endoglucanase activity was found. More than half the strains (50.5 %) showed antifungal activity against at least one of the plant pathogens tested. Compared with other strains in this study, P. citrinum (strain SFC20140101-M662) showed high antifungal activity against both plant pathogens. The results reported here expand our knowledge of marine-derived Penicillium diversity. The relatively high proportion of strains that showed antifungal and enzyme activity demonstrates that marine-derived Penicillium have great potential to be used in the production of natural bioactive products for pharmaceutical and/or industrial use.

  17. 75 FR 78228 - Takes of Marine Mammals Incidental to Specified Activities; Columbia River Crossing Project...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-15

    ... authorization to take marine mammals incidental to bridge construction and demolition activities at the Columbia... (MMPA), NMFS is announcing receipt of CRC's request for the development and implementation of... activities: Replacement of the existing Columbia River bridges with two new structures; Widening of...

  18. 75 FR 5045 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ...) gunnery missions in the Gulf of Mexico (GOM). The USAF's activities are considered military readiness...'' provisions and amended the definition of ``harassment'' as it applies to a ``military readiness activity'' to... IHA and request for 30-day public comment published on January 23, 2006 (71 FR 3474). A 1-year IHA...

  19. Studies in Low-Energy Nuclear Science

    SciTech Connect

    Carl R. Brune; Steven M. Grimes

    2010-01-13

    This report presents a summary of research projects in the area of low energy nuclear reactions and structure, carried out between March 1, 2006 and October 31, 2009 which were supported by U.S. DOE grant number DE-FG52-06NA26187.

  20. Parity violation in low-energy

    SciTech Connect

    Martin Savage

    2001-12-01

    Parity violation in low-energy nuclear observables is included in the pionless effective field theory. The model-independent relation between the parity-violating asymmetry in polarized np -> d gamma and the non-nucleon part of the deuteron anapole moment is discussed. The asymmetry in np -> d gamma computed with KSW power-counting, and recently criticized by Desplanques, is discussed.

  1. 76 FR 41463 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-14

    ...NMFS has received an application from the University of Alaska Geophysics Institute (UAGI) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to conducting a marine geophysical seismic survey in the Arctic Ocean during September- October 2011. Pursuant to the Marine Mammal Protection Act (MMPA), NMFS is requesting comments on its proposal to......

  2. In vitro anti-HMPV activity of meroditerpenoids from marine alga Stypopodium zonale (Dictyotales).

    PubMed

    Mendes, Gabriella; Soares, Angélica Ribeiro; Sigiliano, Lorena; Machado, Fernanda; Kaiser, Carlos; Romeiro, Nelilma; Gestinari, Lísia; Santos, Norma; Romanos, Maria Teresa Villela

    2011-01-01

    In this paper, we evaluated the antiviral activity against HMPV replication of crude extract of the marine algae Stypopodium zonale and of two meroditerpenoids obtained from it, atomaric acid and epitaondiol, and a methyl ester derivative of atomaric acid. Their selectivity indexes were 20.78, >56.81, 49.26 and 12.82, respectively. Compared to ribavirin, the substances showed a relatively low cytotoxicity on LLC-MK2 cells, with a significant antiviral activity, inhibiting at least 90% of viral replication in vitro, which demonstrates the potential of these marine natural products to combat infections caused by HMPV in vitro. PMID:21986522

  3. 76 FR 62378 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-07

    ...) gunnery missions in the Gulf of Mexico (GOM). The USAF's activities are considered military readiness... geographical region'' provisions and amended the definition of ``harassment'' as it applies to a ``military... Notice of Proposed IHA and request for 30-day public comment published on January 23, 2006 (71 FR...

  4. 76 FR 38621 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    .... On May 6, 2011, NMFS published a notice in the Federal Register (76 FR 26255) disclosing the effects... notice for the proposed IHA (76 FR 26255, May 6, 2011). The activities to be conducted have not changed... reader should refer to the proposed IHA notice (76 FR 26255, May 6, 2011), the IHA application, EA,...

  5. 75 FR 44770 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-29

    ... the program in a previous notice for the proposed IHA (75 FR 28568, May 21, ] 2010). The activities to... acoustic source specifications, the reader should refer to the proposed IHA notice (75 FR 28568, May 21... published in the Federal Register on May 21, 2010 (75 FR 28568). During the comment period, NMFS...

  6. 77 FR 12246 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-29

    ... mammals incidental to black abalone (Haliotis cracherodii) research surveys. The first of five IHAs for the specified activities was issued to VanBlaricom on September 23, 2003 (68 FR 57427; October 3, 2003); the most recent of these was issued on January 18, 2008 (73 FR 4841; January 28, 2008),...

  7. 75 FR 41440 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ... blue whales. Response: Section 7 consultation under the Endangered Species Act (ESA) was reinitiated in... sperm and blue whales in addition to the other cetacean species cited in the MMC's comment. The 2007 opinion concluded that those activities were not likely to adversely affect sperm and blue whales....

  8. 75 FR 53672 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-01

    ..., humpback, and fin whales, and is not likely to adversely affect sperm, sei, or blue whales and Kemp's..., and is not likely to adversely affect sperm, sei, or blue whales. National Environmental Policy Act... incidental harassment authorization for this activity pursuant to section 101(a)(5)(D) of the MMPA (74...

  9. 77 FR 25693 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-01

    ... proposed IHA (77 FR 4765, January 31, 2012). The activities to be conducted have not changed between the... refer to the notice of the proposed IHA (77 FR 4765, January 31, 2012), the application, and associated... Federal Register on January 31, 2012 (77 FR 4765). During the 30-day public comment period, NMFS...

  10. 76 FR 9250 - Taking of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ... Fin whale........ Endangered. B. musculus Blue whale....... Endangered. Suborder Odontoceti (toothed... for this activity, which was published in the Federal Register on Tuesday, July 14, 2009 (74 FR 33960... action contained in the proposed rule (74 FR 33960; July 14, 2009; pages 33961-33962) has not...

  11. 77 FR 56613 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ... this notice, the Observatory did not estimate take of endangered humpback, sei, blue, or fin whales or... regional authorization re: 1 Pa \\1\\ population \\2\\ Bryde's whale 1 0.01 \\4\\ 4 Blue whale 0 ... Authorization (77 FR 19242, March 30, 2012). The Observatory's proposed activities have not changed between...

  12. 77 FR 68107 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-15

    ... preliminary determinations and explained the basis for the proposal and preliminary determinations (77 FR... the Notice of Proposed IHA (77 FR 50990, August 23, 2012). Routine shore activity will continue to... additional information on the survey methodology (77 FR 50990, August 23, 2012). That information has...

  13. 78 FR 79403 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-30

    ... proposal and preliminary determinations (78 FR 64918). The notice initiated a 30-day public comment period... incidental to these same proposed activities (77 FR 72327, December 5, 2012). That IHA expired on December 2... ADDRESSES) and the Notice of Proposed IHA (78 FR 64918, October 30, 2013). Community Structure...

  14. 76 FR 46729 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... in the Federal Register notice for the proposed IHA (76 FR 30110; May 24, 2011). That information has... IHA (76 FR 30110; May 24, 2011). That information has not changed and is therefore not repeated here... Register on May 24, 2011 (76 FR 30110). That notice described, in detail, Statoil's proposed activity,...

  15. 75 FR 32379 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ... mammals, readers are encouraged to review NMFS' response to comments on this matter found in 69 FR 74905 (December 14, 2004), 71 FR 43112 (July 31, 2006), 71 FR 50027 (August 24, 2006), and 71 FR 49418 (August 23... of 26 active airguns in 13 clusters. The difference in discharge volume would lead to an...

  16. 78 FR 34069 - Takes of Marine Mammals Incidental to Specified Activities; Marine Geophysical Survey in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... published a notice in the Federal Register (78 FR 17359) making preliminary determinations and proposing to... program in a previous notice for the proposed IHA (78 FR 17359, March 21, 2013). The activities to be... source specifications, the reader should refer to the notice for the proposed IHA (78 FR 17539, March...

  17. 76 FR 30110 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... 410 ft), respectively. Therefore, it is unlikely that such effects would occur during Statoil's... Arctic Ocean or strand as a result of the proposed shallow hazards survey. Potential Effects From Active... have been proposed for Statoil's 2011 open water shallow hazards survey in the Chukchi Sea....

  18. Marine Invertebrate Xenobiotic-Activated Nuclear Receptors: Their Application as Sensor Elements in High-Throughput Bioassays for Marine Bioactive Compounds

    PubMed Central

    Richter, Ingrid; Fidler, Andrew E.

    2014-01-01

    Developing high-throughput assays to screen marine extracts for bioactive compounds presents both conceptual and technical challenges. One major challenge is to develop assays that have well-grounded ecological and evolutionary rationales. In this review we propose that a specific group of ligand-activated transcription factors are particularly well-suited to act as sensors in such bioassays. More specifically, xenobiotic-activated nuclear receptors (XANRs) regulate transcription of genes involved in xenobiotic detoxification. XANR ligand-binding domains (LBDs) may adaptively evolve to bind those bioactive, and potentially toxic, compounds to which organisms are normally exposed to through their specific diets. A brief overview of the function and taxonomic distribution of both vertebrate and invertebrate XANRs is first provided. Proof-of-concept experiments are then described which confirm that a filter-feeding marine invertebrate XANR LBD is activated by marine bioactive compounds. We speculate that increasing access to marine invertebrate genome sequence data, in combination with the expression of functional recombinant marine invertebrate XANR LBDs, will facilitate the generation of high-throughput bioassays/biosensors of widely differing specificities, but all based on activation of XANR LBDs. Such assays may find application in screening marine extracts for bioactive compounds that could act as drug lead compounds. PMID:25421319

  19. Teacher's Activity Guide to Coastal Awareness. Marine Bulletin No. 23.

    ERIC Educational Resources Information Center

    Callaghan, Sara S.

    This teacher's guide was prepared for use with "Down Where the Water Is: A Coastal Awareness Activity Book," as part of the Rhode Island Coastal Resources Management Council's public education program. Contained are instructions on the use of the Activity Book, page-by-page, with glossaries, activity ideas, resources, places to visit, and notes…

  20. Cytotoxicity and antimicrobial activity of marine macro algae (Dictyotaceae and Ulvaceae) from the Persian Gulf.

    PubMed

    Mashjoor, Sakineh; Yousefzadi, Morteza; Esmaeili, Mohamad Ali; Rafiee, Roya

    2016-10-01

    Pharmaceutical industry now accept the worlds ocean which contains a vast array of organisms with unique biological properties, as a major frontier for medical investigation. Bioactive compounds with different modes of action, such as, antiproliferative, antioxidant, antimicrotubule, have been isolated from marine sources, specifically macro and micro algae, and cyanobacteria. The aim of this work was to investigate antimicrobial and cytotoxic activities of the extracts of marine macro algae Ulva flexuosa, Padina antillarum and Padina boergeseni from the northern coasts of the Persian Gulf, Qeshm Island, Iran, against three cell lines including MCF7, HeLa and Vero, as well as their inhibitory effects against a wide array (i.e. n = 11) of pathogenic bacteria and fungi. Antimicrobial activity of the marine macro algal extracts was assessed using a disc diffusion method; an MTT cytotoxicity assay was employed to test the effects of the extracts on each cancer cell line. The algal extracts showed considerable antimicrobial activity against the majority of the tested bacteria and fungi. Both ethyl acetate and methanol extracts at the highest concentration (100 µg/ml) caused cell death, with the IC50 values calculated for each cell type and each algal extracts. Results are exhibited a higher decrease in the viability of the cells treated at the highest concentration of marine macro algal ethyl acetate extracts compared to the methanol extracts (78.9 % death in Vero cells by ethyl acetate extracts from U. flexuosa). Despite, the ethyl acetate extracts with lower dose- response of cells, exhibited better cytotoxic activity than methanol extracts (IC50: 55.26 μg/ml in Vero cells by ethyl acetate extracts from U. flexuosa). Based on the findings, it is concluded that the marine macro algal extracts from the Persian Gulf possess antibacterial and cytotoxic potential, which could be considered for future applications in medicine and identifying novel drugs from the

  1. Marine Invertebrate Metabolites with Anticancer Activities: Solutions to the "Supply Problem".

    PubMed

    Gomes, Nelson G M; Dasari, Ramesh; Chandra, Sunena; Kiss, Robert; Kornienko, Alexander

    2016-05-01

    Marine invertebrates provide a rich source of metabolites with anticancer activities and several marine-derived agents have been approved for the treatment of cancer. However, the limited supply of promising anticancer metabolites from their natural sources is a major hurdle to their preclinical and clinical development. Thus, the lack of a sustainable large-scale supply has been an important challenge facing chemists and biologists involved in marine-based drug discovery. In the current review we describe the main strategies aimed to overcome the supply problem. These include: marine invertebrate aquaculture, invertebrate and symbiont cell culture, culture-independent strategies, total chemical synthesis, semi-synthesis, and a number of hybrid strategies. We provide examples illustrating the application of these strategies for the supply of marine invertebrate-derived anticancer agents. Finally, we encourage the scientific community to develop scalable methods to obtain selected metabolites, which in the authors' opinion should be pursued due to their most promising anticancer activities. PMID:27213412

  2. Marine Invertebrate Metabolites with Anticancer Activities: Solutions to the “Supply Problem”

    PubMed Central

    Gomes, Nelson G. M.; Dasari, Ramesh; Chandra, Sunena; Kiss, Robert; Kornienko, Alexander

    2016-01-01

    Marine invertebrates provide a rich source of metabolites with anticancer activities and several marine-derived agents have been approved for the treatment of cancer. However, the limited supply of promising anticancer metabolites from their natural sources is a major hurdle to their preclinical and clinical development. Thus, the lack of a sustainable large-scale supply has been an important challenge facing chemists and biologists involved in marine-based drug discovery. In the current review we describe the main strategies aimed to overcome the supply problem. These include: marine invertebrate aquaculture, invertebrate and symbiont cell culture, culture-independent strategies, total chemical synthesis, semi-synthesis, and a number of hybrid strategies. We provide examples illustrating the application of these strategies for the supply of marine invertebrate-derived anticancer agents. Finally, we encourage the scientific community to develop scalable methods to obtain selected metabolites, which in the authors’ opinion should be pursued due to their most promising anticancer activities. PMID:27213412

  3. Deep sequencing of subseafloor eukaryotic rRNA reveals active Fungi across marine subsurface provinces.

    PubMed

    Orsi, William; Biddle, Jennifer F; Edgcomb, Virginia

    2013-01-01

    The deep marine subsurface is a vast habitat for microbial life where cells may live on geologic timescales. Because DNA in sediments may be preserved on long timescales, ribosomal RNA (rRNA) is suggested to be a proxy for the active fraction of a microbial community in the subsurface. During an investigation of eukaryotic 18S rRNA by amplicon pyrosequencing, unique profiles of Fungi were found across a range of marine subsurface provinces including ridge flanks, continental margins, and abyssal plains. Subseafloor fungal populations exhibit statistically significant correlations with total organic carbon (TOC), nitrate, sulfide, and dissolved inorganic carbon (DIC). These correlations are supported by terminal restriction length polymorphism (TRFLP) analyses of fungal rRNA. Geochemical correlations with fungal pyrosequencing and TRFLP data from this geographically broad sample set suggests environmental selection of active Fungi in the marine subsurface. Within the same dataset, ancient rRNA signatures were recovered from plants and diatoms in marine sediments ranging from 0.03 to 2.7 million years old, suggesting that rRNA from some eukaryotic taxa may be much more stable than previously considered in the marine subsurface.

  4. Deep Sequencing of Subseafloor Eukaryotic rRNA Reveals Active Fungi across Marine Subsurface Provinces

    PubMed Central

    Orsi, William; Biddle, Jennifer F.; Edgcomb, Virginia

    2013-01-01

    The deep marine subsurface is a vast habitat for microbial life where cells may live on geologic timescales. Because DNA in sediments may be preserved on long timescales, ribosomal RNA (rRNA) is suggested to be a proxy for the active fraction of a microbial community in the subsurface. During an investigation of eukaryotic 18S rRNA by amplicon pyrosequencing, unique profiles of Fungi were found across a range of marine subsurface provinces including ridge flanks, continental margins, and abyssal plains. Subseafloor fungal populations exhibit statistically significant correlations with total organic carbon (TOC), nitrate, sulfide, and dissolved inorganic carbon (DIC). These correlations are supported by terminal restriction length polymorphism (TRFLP) analyses of fungal rRNA. Geochemical correlations with fungal pyrosequencing and TRFLP data from this geographically broad sample set suggests environmental selection of active Fungi in the marine subsurface. Within the same dataset, ancient rRNA signatures were recovered from plants and diatoms in marine sediments ranging from 0.03 to 2.7 million years old, suggesting that rRNA from some eukaryotic taxa may be much more stable than previously considered in the marine subsurface. PMID:23418556

  5. Entanglement creation in low-energy scattering

    SciTech Connect

    Weder, Ricardo

    2011-12-15

    We study the entanglement creation in the low-energy scattering of two particles in three dimensions, for a general class of interaction potentials that are not required to be spherically symmetric. The incoming asymptotic state, before the collision, is a product of two normalized Gaussian states. After the scattering, the particles are entangled. We take as a measure of the entanglement the purity of one of them. We provide a rigorous explicit computation, with error bound, of the leading order of the purity at low energy. The entanglement depends strongly on the difference of the masses. It takes its minimum when the masses are equal, and it increases rapidly with the difference of the masses. It is quite remarkable that the anisotropy of the potential gives no contribution to the leading order of the purity, in spite of the fact that entanglement is a second-order effect.

  6. Low energy ion loss at Mars

    NASA Astrophysics Data System (ADS)

    Curry, S.; Liemohn, M.; Fang, X.; Ma, Y.

    2012-04-01

    Current data observations and modeling efforts have indicated that the low-energy pick-up ions on Mars significantly contribute to the overall escape rate. Due to the lack of a dipole magnetic field, the solar wind directly interacts with the dayside upper atmosphere causing particles to be stripped away. In this study, we use a 3-D Monte Carlo test particle simulation with virtual detectors to observe low energy ions (< 50 eV) in the Mars space environment. We will present velocity space distributions that can capture the asymmetric and non-gyrotropic features of particle motion. The effect of different solar conditions will also be discussed with respect to ion fluxes at various spatial locations as well as overall loss in order to robustly describe the physical processes controlling the distribution of planetary ions and atmospheric escape.

  7. Low energy cyclotron for radiocarbon dating

    SciTech Connect

    Welch, J.J.

    1985-01-01

    The author built and tested a low energy cyclotron for radiocarbon dating similar to a conventional mass spectrometer. These tests clearly show that with the addition of a conventional ion source, the low energy cyclotron can perform the extremely high sensitivity /sup 14/C measurements that are now done at accelerator facilities. The author found that no significant background is present when the cyclotron is tuned to accelerate /sup 14/C negative ions and the transmission efficiency is adequate to perform radiocarbon dating on milligram samples of carbon. The internal ion source used did not produce sufficient current to detect /sup 14/C directly at modern concentrations. The author shows how a conventional carbon negative ion source located outside the cyclotron magnet, would produce sufficient beam and provide for quick sample changing to make radiocarbon dating milligram samples with a modest laboratory instrument feasible.

  8. Low Energy Transfer to the Moon

    NASA Astrophysics Data System (ADS)

    Koon, W. S.; Lo, M. W.; Marsden, J. E.; Ross, S. D.

    In 1991, the Japanese Hiten mission used a low energy transfer with a ballistic capture at the Moon which required less Δ V than a standard Hohmann transfer. In this paper, we apply the dynamical systems techniques developed in our earlier work to reproduce systematically a Hiten-like mission. We approximate the Sun-Earth-Moon-spacecraft 4-body system as two 3-body systems. Using the invariant manifold structures of the Lagrange points of the 3-body systems, we are able to construct low energy transfer trajectories from the Earth which execute ballistic capture at the Moon. The techniques used in the design and construction of this trajectory may be applied in many situations.

  9. Regulatory Assistance, Stakeholder Outreach, and Coastal and Marine Spatial Planning Activities in Support of Marine and Hydrokinetic Energy Deployment

    SciTech Connect

    Geerlofs, Simon H.; Copping, Andrea E.; Van Cleve, Frances B.; Blake, Kara M.; Hanna, Luke A.

    2011-09-30

    This fiscal year 2011 progress report summarizes activities carried out under DOE Water Power Task 2.1.7, Permitting and Planning. Activities under Task 2.1.7 address the concerns of a wide range of stakeholders with an interest in the development of the marine and hydrokinetic (MHK) energy industry, including regulatory and resource management agencies, tribes, nongovernmental organizations, and industry. Objectives for Task 2.1.7 are the following: • to work with stakeholders to streamline the MHK regulatory permitting process • to work with stakeholders to gather information on needs and priorities for environmental assessment of MHK development • to communicate research findings and directions to the MHK industry and stakeholders • to engage in spatial planning processes in order to further the development of the MHK industry. These objectives are met through three subtasks, each of which is described in this report: • 2.1.7.1—Regulatory Assistance • 2.1.7.2—Stakeholder Outreach • 2.1.7.3—Coastal and Marine Spatial Planning. As MHK industry partners work with the regulatory community and stakeholders to plan, site, permit, and license MHK technologies, they have an interest in a predictable, efficient, and transparent process. Stakeholders and regulators have an interest in processes that result in sustainable use of ocean space with minimal effects to existing ocean users. Both stakeholders and regulators have an interest in avoiding legal challenges by meeting the intent of federal, state, and local laws that govern siting and operation of MHK technologies. The intention of work under Task 2.1.7 is to understand and work to address these varied interests, reduce conflict, identify efficiencies, and ultimately reduce the regulatory costs, time, and potential environmental impacts associated with developing, siting, permitting, and deploying MHK systems.

  10. PHYSICS WITH ULTRA-LOW ENERGY ANTIPROTONS

    SciTech Connect

    M. HOLZSCHEITER

    2001-02-01

    In this report the author describes the current status of the antiproton deceleration (AD) facility at CERN, and highlights the physics program with ultra-low energy antiproton at this installation. He also comments on future possibilities provided higher intensity antiproton beams become available at Fermilab, and review possibilities for initial experiments using direct degrading of high energy antiprotons in material has been developed and proven at CERN.

  11. Seesaw scale from low-energy parameters

    SciTech Connect

    He Xiaogang

    2012-07-27

    Seesaw models can provide explanations why neutrino are so much lighter than their charged partners. However, A priori, the seesaw scale is not determined. In this talk, I report work published in Ref.[1] by Law, Volkas and myself which shows that it is possible to construct theoretical models where the seesaw scale is completely determined in terms of low-energy fermion mass, mixing angles and CP-violating phases observable quantities.

  12. Targeting Low-Energy Ballistic Lunar Transfers

    NASA Technical Reports Server (NTRS)

    Parker, Jeffrey S.

    2010-01-01

    Numerous low-energy ballistic transfers exist between the Earth and Moon that require less fuel than conventional transfers, but require three or more months of transfer time. An entirely ballistic lunar transfer departs the Earth from a particular declination at some time in order to arrive at the Moon at a given time along a desirable approach. Maneuvers may be added to the trajectory in order to adjust the Earth departure to meet mission requirements. In this paper, we characterize the (Delta)V cost required to adjust a low-energy ballistic lunar transfer such that a spacecraft may depart the Earth at a desirable declination, e.g., 28.5(white bullet), on a designated date. This study identifies the optimal locations to place one or two maneuvers along a transfer to minimize the (Delta)V cost of the transfer. One practical application of this study is to characterize the launch period for a mission that aims to launch from a particular launch site, such as Cape Canaveral, Florida, and arrive at a particular orbit at the Moon on a given date using a three-month low-energy transfer.

  13. Low-energy electron interactions with biomolecules

    NASA Astrophysics Data System (ADS)

    Winstead, Carl

    2012-06-01

    Low-energy electron interactions with biomolecules have been the focus of sustained attention over the past decade. The demonstration by Sanche and coworkers that even subexcitation and subionization electrons can induce strand breaks in DNA opened a new frontier in understanding radiation damage to living systems. Many studies of DNA subunits and their analogues, both experimental and theoretical, have elucidated likely mechanisms by which slow electrons attach to and disrupt DNA, although the full picture is far from clear and some elements of it remain controversial. Increasing attention is also being given to low-energy electron collisions with amino acids in order to explore possible mechanisms of electron-mediated radiation damage to proteins. In a completely different context, electron-biomolecule collisions are fundamental to spark ignition and cumbustion of biofuels such as methanol and ethanol. Not to be overlooked, either, is the simplest but most ubiquitous biomolecule of all, water, whose low-energy electron cross sections remain surprisingly ill-characterized. This talk will survery recent ab initio computational studies using the Schwinger multichannel method of DNA- and protein-related molecules, alcohols, and water. Much of the work to be presented was carried out in collaboration with experimentalists who undertook complementary measurements, allowing for useful comparisons to be made. Although the primary focus will be on electronically elastic collisions relevant to dissociative attachment and electron transport, electron-impact excitation cross sections for water will be presented and discussed.

  14. 50 CFR 18.27 - Regulations governing small takes of marine mammals incidental to specified activities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... contained in the Act and in 50 CFR 18.3 and unless the context otherwise requires, in this section: Citizens.... (Complete definition of take is contained in 50 CFR 18.3.) Negligible impact is an impact resulting from the... marine mammals incidental to specified activities. 18.27 Section 18.27 Wildlife and Fisheries...

  15. 50 CFR 18.27 - Regulations governing small takes of marine mammals incidental to specified activities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... contained in the Act and in 50 CFR 18.3 and unless the context otherwise requires, in this section: Citizens.... (Complete definition of take is contained in 50 CFR 18.3.) Negligible impact is an impact resulting from the... marine mammals incidental to specified activities. 18.27 Section 18.27 Wildlife and Fisheries...

  16. 50 CFR 18.27 - Regulations governing small takes of marine mammals incidental to specified activities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... contained in the Act and in 50 CFR 18.3 and unless the context otherwise requires, in this section: Citizens.... (Complete definition of take is contained in 50 CFR 18.3.) Negligible impact is an impact resulting from the... marine mammals incidental to specified activities. 18.27 Section 18.27 Wildlife and Fisheries...

  17. Only One Ocean: Marine Science Activities for Grades 5-8. Teacher's Guide.

    ERIC Educational Resources Information Center

    Halversen, Catherine; Strang, Craig

    This guide was designed by the Marine Activities, Resources & Education (MARE) Program through the Great Explorations in Math and Science (GEMS) ongoing curriculum development program for middle school students. This GEMS guide addresses the concepts of the interconnectedness of the ocean basins, respect for organisms, oceanography, physical…

  18. 75 FR 8677 - Takes of Marine Mammals Incidental to Specified Activities; Seabird and Pinniped Research...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... of the activity in the notice of the proposed IHA (74 FR 61109, November 23, 2009). No changes have..., 2009 (74 FR 61109). During the 30-day comment period, NMFS received a letter from the Marine Mammal... coordinating visits to the island) as described in NMFS' November 23, 2009 (74 FR 61109), notice of...

  19. 76 FR 46724 - Takes of Marine Mammals Incidental to Specified Activities; Seabird and Pinniped Research...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... areas of the national park. Description of the Specified Activity PRBO will conduct seabird and pinniped... Seashore The National Park Service in collaboration with PRBO monitors seabird breeding and roosting... National Oceanic and Atmospheric Administration RIN 0648-XA534 Takes of Marine Mammals Incidental...

  20. 76 FR 10564 - Takes of Marine Mammals Incidental to Specified Activities; St. George Reef Light Station...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-25

    ... of a report with a data table, any other significant observations related to marine mammals, and a... notice for the proposed IHA (75 FR 80471 December 22, 2010). The planned activities have not changed... reader should refer to the proposed IHA notice (75 FR 8047, December 22, 2010). Comments and...

  1. 77 FR 39999 - Takes of Marine Mammals Incidental to Specified Activities; Pile Placement for Fishermen's...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-06

    ..., NMFS issued a notice in the Federal Register on March 13, 2012 (77 FR 14736), requesting comments from... specified activity may be found in NMFS' proposed IHA notice in the Federal Register (77 FR 14736, March 13... FR 14736). During the 30-day public comment period, the Marine Mammal Commission...

  2. Total Synthesis and Structure-Activity Relationship of Glycoglycerolipids from Marine Organisms

    PubMed Central

    Zhang, Jun; Li, Chunxia; Yu, Guangli; Guan, Huashi

    2014-01-01

    Glycoglycerolipids occur widely in natural products, especially in the marine species. Glycoglycerolipids have been shown to possess a variety of bioactivities. This paper will review the different methodologies and strategies for the synthesis of biological glycoglycerolipids and their analogs for bioactivity assay. In addition, the bioactivities and structure-activity relationship of the glycoglycerolipids are also briefly outlined. PMID:24945415

  3. 77 FR 24471 - Takes of Marine Mammals Incidental to Specified Activities; Russian River Estuary Management...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-24

    ... IHA, valid for a period of one year, on April 1, 2010 (75 FR 17382), and was subsequently issued a second IHA for incidental take associated with the same activities on April 21, 2011 (76 FR 23306..., 2012 (77 FR 15722). During the 30-day comment period, NMFS received a letter from the Marine...

  4. 78 FR 36527 - Taking of Marine Mammals Incidental to Specified Activities; Construction at Bremerton Ferry...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-18

    ... Ferry Terminal is provided in the Federal Register notice for the proposed IHA (78 FR 11844; February 20... Federal Register notice for the proposed IHA (78 FR 11844; February 20, 2013). Please refer to that... FR 11844). That notice described, in detail, WSDOT's activity, the marine mammal species that may...

  5. Activated chemical defense in marine sponges--a case study on Aplysinella rhax.

    PubMed

    Thoms, Carsten; Schupp, Peter J

    2008-09-01

    Activated chemical defense, i.e., the rapid conversion of precursor molecules to defensive compounds following tissue damage, has been well documented for terrestrial and marine plants; but evidence for its presence in sessile marine invertebrates remains scarce. We observed a wound-activated conversion of psammaplin A sulfate to psammaplin A in tissue of the tropical sponge Aplysinella rhax. The conversion is rapid (requiring only seconds), the turnover rate increases with increasing wounding activity (e.g., approximately 20% after tissue stabbing vs. approximately 85% after tissue grinding), and is likely enzyme-catalyzed (no reaction in the absence of water and inhibition of the conversion by heat). Fish feeding assays with the pufferfish Canthigaster solandri, an omnivorous sponge predator, revealed an increased anti-feeding activity by the conversion product psammaplin A compared to the precursor psammaplin A sulfate. We propose that the wound-activated formation of psammaplin A in A. rhax is an activated defense targeted against predator species that are not efficiently repelled by the sponge's constitutive chemical defense. Recent observations of conversion reactions also in other sponge species indicate that more activated defenses may exist in this phylum. Based on the findings of this study, we address the question whether activated defenses may be more common in sponges--and perhaps also in other sessile marine invertebrates--than hitherto believed.

  6. Current Suicidal Ideation among Treatment-Engaged Active Duty Soldiers and Marines

    PubMed Central

    Zimmerman, Lindsey; Villatte, Jennifer L.; Kerbrat, Amanda H.; Atkins, David C.; Flaster, Aaron; Comtois, Kate A.

    2015-01-01

    We examined suicidal ideation among 399 active duty Soldiers and Marines engaged in mental health treatment. Using a generalized linear model controlling for demographic and military factors, depression, and positive traumatic brain injury screen, we confirmed our hypothesis that self-report measures of current PTSD symptoms uniquely predicted suicidal ideation. The association between PTSD severity and suicidal ideation was moderated by gender with women at higher risk as PTSD severity increased. Female Soldiers and Marines with high levels of PTSD should receive additional monitoring and intervention. Self-report measures may aid with risk assessment and identify symptom-related distress associated with suicide risk. PMID:27170848

  7. A model to predict anti-tuberculosis activity: value proposition for marine microorganisms.

    PubMed

    Liu, Miaomiao; Grkovic, Tanja; Zhang, Lixin; Liu, Xueting; Quinn, Ronald J

    2016-08-01

    The development of new antibiotics effective against all strains of tuberculosis (TB) is needed. To evaluate the potential of marine microbe-derived natural products as anti-TB leads, we analyzed and compared the physico-chemical properties of 39 current TB drugs and candidates against 60 confirmed mycobacteria-active natural products. We showed that anti-TB natural products sourced from marine microbes have a large overlap with TB drug-like space. A model to predict potential anti-TB drugs is proposed. PMID:27406906

  8. RHIC low energy beam loss projections

    SciTech Connect

    Satogata,T.

    2009-08-01

    For RHIC low-energy operations, we plan to collide Au beams with energies of E = 2:5-10 GeV/u in RHIC. Beams are injected into collision optics, and RHIC runs as a storage ring with no acceleration. At these low energies, observed beam lifetimes are minutes, with measured beam lifetimes of 3.5 min (fast) and 50 min (slow) at E=4.6 GeV/u in the March 2008 test run. With these lifetimes we can operate RHIC as a storage ring to produce reasonable integrated luminosity. This note estimates beam losses and collimator/dump energy deposition in normal injection modes of low energy operation. The main question is whether a normal injection run is feasible for an FY10 10-15 week operations run from a radiation safety perspective. A peripheral question is whether continuous injection operations is feasible from a radiation safety perspective. In continuous injection mode, we fill both rings, then continuously extract and reinject the oldest bunches that have suffered the most beam loss to increase the overall integrated luminosity. We expect to gain a factor of 2-3 in integrated luminosity from continuous injection at lowest energies if implemented[1]. Continuous injection is feasible by FY11 from an engineering perspective given enough effort, but the required extra safety controls and hardware dose risk make it unappealing for the projected luminosity improvement. Low-energy electron cooling will reduce beam losses by at least an order of magnitude vs normal low-energy operations, but low energy cooling is only feasible in the FY13 timescale and therefore beyond the scope of this note. For normal injection low energy estimates we assume the following: (1) RHIC beam total energies are E=2.5-10 GeV/u. (Continuous injection mode is probably unnecessary above total energies of E=7-8 GeV/u.); (2) RHIC operates only as a storage ring, with no acceleration; (3) 110 bunches of about 0.5-1.0 x 10{sup 9} initial bunch intensities (50-100% injection efficiency, likely conservative

  9. Neutron activation analysis of major, minor, and trace elements in marine sediments

    SciTech Connect

    Stone, S.F.; Zeisler, R.; Koster, B.J.

    1988-01-01

    Neutron activation analysis (NAA) techniques are well established in the multielement assay of geological materials. Similarly, applications of NAA to the analysis of marine sediments have been described. The different emphasis on elemental composition in studying and monitoring the health of the environment, however, presents a new challenge to the analyst. To investigate as many elements as possible, previous multielement procedures need to be reevaluated and modified. In this work, the authors have utilized the NAA steps of a recently developed sequential analysis procedure that obtained concentrations for 45 biological and pollutant elements in marine bivalves. This procedure, with modification, was applied to samples of marine sediments collected for the National Oceanic and Atmospheric Administration (NOAA) National Status and Trends (NS T) specimen banking program.

  10. Ecological and Pharmacological Activities of Antarctic Marine Natural Products.

    PubMed

    Avila, Conxita

    2016-06-01

    Antarctic benthic communities are regulated by abundant interactions of different types among organisms, such as predation, competition, etc. Predators are usually sea stars, with omnivorous habits, as well as other invertebrates. Against this strong predation pressure, many organisms have developed all sorts of defensive strategies, including chemical defenses. Natural products are thus quite common in Antarctic organisms with an important ecological and pharmacological potential. In this paper, the chemical defenses of the Antarctic organisms studied during the ECOQUIM and ACTIQUIM projects, as well as their pharmacological potential, are reviewed. For the ecological defenses, predation against the sea star Odontaster validus is analyzed and evaluated along depth gradients as well as considering the lifestyle of the organisms. For the pharmacological activity, the anticancer, anti-inflammatory, and antibacterial activities tested are evaluated here. Very often, only crude extracts or fractions have been tested so far, and therefore, the natural products responsible for such activities remain yet to be identified. Even if the sampling efforts are not uniform along depth, most ecologically active organisms are found between 200 and 500 m depth. Also, from the samples studied, about four times more sessile organisms possess chemical defenses against the sea star than the vagile ones; these represent 50 % of sessile organisms and 35 % of the vagile ones, out of the total tested, being active. Pharmacological activity has not been tested uniformly in all groups, but the results show that relevant activity is found in different phyla, especially in Porifera, Cnidaria, Bryozoa, and Tunicata, but also in others. No relationship between depth and pharmacological activity can be established with the samples tested so far. More studies are needed in order to better understand the ecological relationships among Antarctic invertebrates mediated by natural products and

  11. Ecological and Pharmacological Activities of Antarctic Marine Natural Products.

    PubMed

    Avila, Conxita

    2016-06-01

    Antarctic benthic communities are regulated by abundant interactions of different types among organisms, such as predation, competition, etc. Predators are usually sea stars, with omnivorous habits, as well as other invertebrates. Against this strong predation pressure, many organisms have developed all sorts of defensive strategies, including chemical defenses. Natural products are thus quite common in Antarctic organisms with an important ecological and pharmacological potential. In this paper, the chemical defenses of the Antarctic organisms studied during the ECOQUIM and ACTIQUIM projects, as well as their pharmacological potential, are reviewed. For the ecological defenses, predation against the sea star Odontaster validus is analyzed and evaluated along depth gradients as well as considering the lifestyle of the organisms. For the pharmacological activity, the anticancer, anti-inflammatory, and antibacterial activities tested are evaluated here. Very often, only crude extracts or fractions have been tested so far, and therefore, the natural products responsible for such activities remain yet to be identified. Even if the sampling efforts are not uniform along depth, most ecologically active organisms are found between 200 and 500 m depth. Also, from the samples studied, about four times more sessile organisms possess chemical defenses against the sea star than the vagile ones; these represent 50 % of sessile organisms and 35 % of the vagile ones, out of the total tested, being active. Pharmacological activity has not been tested uniformly in all groups, but the results show that relevant activity is found in different phyla, especially in Porifera, Cnidaria, Bryozoa, and Tunicata, but also in others. No relationship between depth and pharmacological activity can be established with the samples tested so far. More studies are needed in order to better understand the ecological relationships among Antarctic invertebrates mediated by natural products and

  12. Investigation of Marine-Derived Fungal Diversity and Their Exploitable Biological Activities

    PubMed Central

    Hong, Joo-Hyun; Jang, Seokyoon; Heo, Young Mok; Min, Mihee; Lee, Hwanhwi; Lee, Young Min; Lee, Hanbyul; Kim, Jae-Jin

    2015-01-01

    Marine fungi are potential producers of bioactive compounds that may have pharmacological and medicinal applications. Fungi were cultured from marine brown algae and identified using multiple target genes to confirm phylogenetic placement. These target genes included the internal transcribed spacer (ITS), the nuclear large subunit (LSU), and the β-tubulin region. Various biological activities of marine-derived fungi were evaluated, including their antifungal, antioxidant and cellulolytic enzyme activities. As a result, a total of 50 fungi was isolated from the brown algae Sargassum sp. Among the 50 isolated fungi, Corollospora angusta was the dominant species in this study. The genus Arthrinium showed a relatively strong antifungal activity to all of the target plant pathogenic fungi. In particular, Arthrinium saccharicola KUC21221 showed high radical scavenging activity and the highest activities in terms of filter paper units (0.39 U/mL), endoglucanase activity (0.38 U/mL), and β-glucosidase activity (1.04 U/mL). PMID:26133554

  13. Investigation of Marine-Derived Fungal Diversity and Their Exploitable Biological Activities.

    PubMed

    Hong, Joo-Hyun; Jang, Seokyoon; Heo, Young Mok; Min, Mihee; Lee, Hwanhwi; Lee, Young Min; Lee, Hanbyul; Kim, Jae-Jin

    2015-07-01

    Marine fungi are potential producers of bioactive compounds that may have pharmacological and medicinal applications. Fungi were cultured from marine brown algae and identified using multiple target genes to confirm phylogenetic placement. These target genes included the internal transcribed spacer (ITS), the nuclear large subunit (LSU), and the β-tubulin region. Various biological activities of marine-derived fungi were evaluated, including their antifungal, antioxidant and cellulolytic enzyme activities. As a result, a total of 50 fungi was isolated from the brown algae Sargassum sp. Among the 50 isolated fungi, Corollospora angusta was the dominant species in this study. The genus Arthrinium showed a relatively strong antifungal activity to all of the target plant pathogenic fungi. In particular, Arthrinium saccharicola KUC21221 showed high radical scavenging activity and the highest activities in terms of filter paper units (0.39 U/mL), endoglucanase activity (0.38 U/mL), and β-glucosidase activity (1.04 U/mL). PMID:26133554

  14. The anti-biofilm activity secreted by a marine Pseudoalteromonas strain.

    PubMed

    Klein, Géraldine L; Soum-Soutéra, Emmanuelle; Guede, Zakoua; Bazire, Alexis; Compère, Chantal; Dufour, Alain

    2011-09-01

    Bacterial biofilms occur on all submerged structures in marine environments. The authors previously reported that the marine bacterium Pseudoalteromonas sp. 3J6 secretes antibiofilm activity. Here, it was discovered that another Pseudoalteromonas sp. strain, D41, inhibited the development of strain 3J6 in mixed biofilms. Confocal laser scanning microscope observations revealed that the culture supernatant of strain D41 impaired biofilm formation of strain 3J6 and another marine bacterium. A microtiter plate assay of the antibiofilm activity was set up and validated with culture supernatants of Pseudoalteromonas sp. 3J6. This assay was used to determine the spectra of action of strains D41 and 3J6. Each culture supernatant impaired the biofilm development of 13 marine bacteria out of 18. However, differences in the spectra of action and the physical behaviours of the antibiofilm molecules suggest that the latter are not identical. They nevertheless share the originality of being devoid of antibacterial activity against planktonic bacteria. PMID:21895460

  15. Antialgal and antilarval activities of bioactive compounds extracted from the marine dinoflagellate Amphidinium carterae

    NASA Astrophysics Data System (ADS)

    Kong, Xianyu; Han, Xiurong; Gao, Min; Su, Rongguo; Wang, Ke; Li, Xuzhao; Lu, Wei

    2016-09-01

    With the global ban on the application of organotin-based marine coatings by the International Maritime Organization, the development of environmentally friendly, low-toxic and nontoxic antifouling compounds for marine industries has become an urgent need. Marine microorganisms have been considered as a potential source of natural antifoulants. In this study, the antifouling potential of marine dinoflagellate Amphidinium carterae, the toxic and red-tide microalgae, was investigated. We performed a series of operations to extract the bioactive substances from Amphidinium carterae and tested their antialgal and antilarval activities. The crude extract of Amphidinium carterae showed significant antialgal activity and the EC50 value against Skeletonema costatum was 55.4 μg mL-1. After purification, the isolated bioactive substances (the organic extract C) exhibited much higher antialgal and antilarval activities with EC50 of 12.9 μg mL-1 against Skeletonema costatum and LC50 of 15.1 μg mL-1 against Amphibalanus Amphitrite larvae. Subsequently, IR, Q-TOFMS, and GC-MS were utilized for the structural elucidation of the bioactive compounds, and a series of unsaturated and saturated 16- to 22-carbon fatty acids were detected. The data suggested the bioactive compounds isolated from Amphidinium carterae exhibited a significant inhibiting effect against the diatom Skeletonema costatum and Amphibalanus Amphitrite larvae, and could be substitutes for persistent, toxic antifouling compounds.

  16. A Case of Superior Mesenteric Artery Syndrome in a Healthy Active Duty Marine.

    PubMed

    Thota, Darshan; Portouw, Steven J; Bruner, David I

    2015-10-01

    Superior mesenteric artery (SMA) syndrome is an uncommon disorder that can lead to small bowel obstructions or perforations. Typical populations include young females with anorexia. However, there have been a few reports of healthy males with acute vomiting reported to have SMA syndrome. Our case report highlights an active duty Marine who developed SMA syndrome and the importance of recognizing this disease given the severity in delay of diagnosis in population of young healthy active duty members.

  17. Marine Biology Field Trip Sites. Ocean Related Curriculum Activities.

    ERIC Educational Resources Information Center

    Pauls, John

    The ocean affects all of our lives. Therefore, awareness of and information about the interconnections between humans and oceans are prerequisites to making sound decisions for the future. Project ORCA (Ocean Related Curriculum Activities) has developed interdisciplinary curriculum materials designed to meet the needs of students and teachers…

  18. 75 FR 72807 - Taking of Marine Mammals Incidental to Specified Activities; U.S. Marine Corps Training Exercises...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-26

    ... mammals by harassment. Section 101(a)(5)(D) establishes a 45-day time limit for NMFS review of an... for this action (75 FR 32398; June 8, 2010). Description of Marine Mammals in the Area of the... action (75 FR 32398; June 8, 2010). Effects on Marine Mammals As mentioned previously, with respect...

  19. Low-energy proton capture reactions

    SciTech Connect

    Lipoglavsek, M.; Cvetinovic, A.; Gajevic, J.; Likar, A.; Vavpetic, P.; Petrovic, T.

    2014-05-09

    An overview of experimental problems in measuring the cross sections for (p,γ) and (p,n) reactions at low energies is given with a specific emphasis on electron screening in metallic targets. Thick target γ-ray and neutron yields are compared for Ni and NiO targets, V and VO{sub 2} targets and Mn and MnO targets. The {sup 1}H({sup 7}Li,α){sup 4}He reaction was studied in inverse kinematics with hydrogen loaded into Pd and PdAg alloy foils from gas phase. Based on these results, a new approach to electron screening in nuclear reactions is suggested.

  20. Low energy fission: dynamics and scission configurations

    NASA Astrophysics Data System (ADS)

    Goutte, H.; Berger, J.-F.; Gogny, D.; Younes, W.

    2005-11-01

    In the first part of this paper we recall a recent study concerning low energy fission dynamics. Propagation is made by use of the Time Dependent Generator Coordinate Method, where the basis states are taken from self-consistent Hartree-Fock-Bogoliubov calculations with the Gogny force. Theoretical fragment mass distributions are presented and compared with the evaluation made by Wahl. In the second part of this paper, new results concerning scission configurations are shown. Deviations of the fission fragment proton numbers from the Unchanged Charge Distribution prescription and fission fragment deformations are discussed.

  1. FLSR - The Frankfurt low energy storage ring

    NASA Astrophysics Data System (ADS)

    Stiebing, K. E.; Alexandrov, V.; Dörner, R.; Enz, S.; Kazarinov, N. Yu.; Kruppi, T.; Schempp, A.; Schmidt Böcking, H.; Völp, M.; Ziel, P.; Dworak, M.; Dilfer, W.

    2010-02-01

    An electrostatic storage ring for low-energy ions with a design energy of 50 keV is presently being set up at the Institut für Kernphysik der Johann Wolfgang Goethe-Universität Frankfurt am Main, Germany (IKF). This new device will provide a basis for new experiments on the dynamics of ionic and molecular collisions, as well as for high precision and time resolved laser spectroscopy. In this article, the design parameters of this instrument are reported.

  2. The low energy booster project status

    SciTech Connect

    Tuttle, G.W.

    1993-05-01

    In order to achieve the required injection momentum, the Superconducting Super Collider (SSC) has an accelerator chain comprised of a Linear Accelerator and three synchrotrons. The Low Energy Booster (LEB) is the first synchrotron in this chain. The LEB project has made significant progress in the development of major subsystems and conventional construction. This paper briefly reviews the performance requirements of the LEB and describes significant achievements in each of the major subsystem areas. Highlighted among these achievements are the LEB foreign collaborations with the Budker Institute of Nuclear Physics (BINP) located in Novosibirsk, Russia.

  3. Low energy signatures of nonlocal field theories

    NASA Astrophysics Data System (ADS)

    Belenchia, Alessio; Benincasa, Dionigi M. T.; Martín-Martínez, Eduardo; Saravani, Mehdi

    2016-09-01

    The response of inertial particle detectors coupled to a scalar field satisfying nonlocal dynamics described by nonanalytic functions of the d'Alembertian operator □ is studied. We show that spontaneous emission processes of a low energy particle detector are very sensitive to high-energy nonlocality scales. This allows us to suggest a nuclear physics experiment (˜MeV energy scales) that outperforms the sensitivity of LHC experiments by many orders of magnitude. This may have implications for the falsifiability of theoretical proposals of quantum gravity.

  4. Anti-inflammatory activity in mice of extracts from Mediterranean marine invertebrates.

    PubMed

    Herencia, F; Ubeda, A; Ferrándiz, M L; Terencio, M C; Alcaraz, M J; García-Carrascosa, M; Capaccioni, R; Payá, M

    1998-01-01

    The effects of dichloromethane and methanol extracts from the marine invertebrates Leptogorgia ceratophyta, Holothuria tubulosa, Coscinasterias tenuispina and Phallusia fumigata on carrageenan-induced paw oedema in mice were investigated. The dichloromethane extract of Coscinasterias tenuispina and the methanol extract of Holothuria tubulosa administered p.o. at 50, 100 and 150 mg/kg, inhibited oedema in a dose-dependent manner 3 h after administration of carrageenan. Both extracts partially decreased elastase activity and PGE2 levels measured in homogenates from inflamed paws, without affecting the levels of this prostanoid present in stomach homogenates. As observed with the selective inhibitor NS398, both extracts can decrease cyclo-oxygenase activity in inflamed tissues but do not modify the constitutive cyclo-oxygenase enzyme. Therefore, these extracts represent new marine resources for the isolation of novel agents active on inflammatory conditions.

  5. Distribution of Cathepsin D Activity between Lysosomes and a Soluble Fraction of Marinating Brine.

    PubMed

    Szymczak, Mariusz

    2016-08-01

    This paper is the first ever to describe the phenomenon of bimodal distribution of cathepsin D in the lysosomal and soluble fractions of brine left after herring marinating. Up to 2 times higher cathepsin D activity was observed in the lysosome fraction. Activity of cathepsin D in brine increased according to the logarithmic function during low frequency-high power ultrasounds treatment or according to the linear function after multiple freezing-thawing of brine. Activity enhancement was achieved only in the brine devoid of lipids and suspension. Study results show also that measurement of lysosomal cathepsin D activity in the marinating brine requires also determining cathepsin E activity. Decreasing pore size of microfilter from 2.7 to 0.3 μm significantly reduced the lysosome content in the brine. The presence of lysosomes and the possibility of their separation as well as the likely release of cathepsins shall be considered during industrial application of the marinating brine, as new cathepsins preparations in fish and meat technology. PMID:27351340

  6. Defence force activities in marine protected areas: environmental management of Shoalwater Bay Training Area, Queensland, Australia

    NASA Astrophysics Data System (ADS)

    Wu, Wen; Wang, Xiaohua; Paull, David; Kesby, Julie

    2010-05-01

    Environmental management of military activities is of growing global concern by defence forces. As one of the largest landholders in Australia, the Australian Defence Force (ADF) is increasingly concerned with sustainable environmental management. This paper focuses on how the ADF is maintaining effective environmental management, especially in environmentally sensitive marine protected areas. It uses Shoalwater Bay Training Area (SWBTA) as a research example to examine environmental management strategies conducted by the ADF. SWBTA is one of the most significant Defence training areas in Australia, with a large number of single, joint and combined military exercises conducted in the area. With its maritime component contained in the Great Barrier Reef Marine Park (GBRMP), the Great Barrier Reef World Heritage Area (GBRWHA), and abutting Queensland’s State Marine Parks, it has high protection values. It is therefore vital for the ADF to adopt environmentally responsible management while they are conducting military activities. As to various tools employed to manage environmental performance, the ISO 14001 Environmental Management System (EMS) is widely used by the ADF. This paper examines military activities and marine environmental management within SWBTA, using the Talisman Saber (TS) exercise series as an example. These are extensive joint exercises conducted by the ADF and the United States defence forces. The paper outlines relevant legislative framework and environmental policies, analyses how the EMS operates in environmental management of military activities, and how military activities comply with these regulations. It discusses the implementation of the ADF EMS, including risk reduction measures, environmental awareness training, consultation and communication with stakeholders. A number of environmental management actions used in the TS exercises are presented to demonstrate the EMS application. Our investigations to this point indicate that the ADF is

  7. The Telescope Array's Low Energy Extension: TALE

    NASA Astrophysics Data System (ADS)

    Matthews, John

    2009-05-01

    A great deal of information about the sources of ultra high energy cosmic rays exists encoded in the energy spectrum. There are three spectral features in the ultra high energy regime (the second knee, the ankle, and the GZK cut-off). An important composition change also occurs in this energy range. The Telescope Array (TA) is a large area ultra high energy cosmic ray observatory built and operated by groups from the US, Japan, Korea, and Russia. The existing part of the Telescope Array already has good efficiency above the ankle (˜10^18.5 eV). These detectors are already in the field collecting data. The TA Low Energy Extension (TALE) refers to the detectors devoted to the ``low energy'' portion of the spectrum - 10^16.5 - 10^19 eV. The aim of TA/TALE is to understand the origin of cosmic rays and to study their composition over a broad energy range. We will introduce the detector components and discuss the opportunities.

  8. Low Energy Ion-Molecule Reactions

    SciTech Connect

    James M. Farrar

    2004-05-01

    This objective of this project is to study the dynamics of the interactions of low energy ions important in combustion with small molecules in the gas phase and with liquid hydrocarbon surfaces. The first of these topics is a long-standing project in our laboratory devoted to probing the key features of potential energy surfaces that control chemical reactivity. The project provides detailed information on the utilization of specific forms of incident energy, the role of preferred reagent geometries, and the disposal of total reaction energy into product degrees of freedom. We employ crossed molecular beam methods under single collision conditions, at collision energies from below one eV to several eV, to probe potential surfaces over a broad range of distances and interaction energies. These studies allow us to test and validate dynamical models describing chemical reactivity. Measurements of energy and angular distributions of the reaction products with vibrational state resolution provide the key data for these studies. We employ the crossed beam low energy mass spectrometry methods that we have developed over the last several years.

  9. Low Energy Nuclear Reactions: 2007 Update

    NASA Astrophysics Data System (ADS)

    Krivit, Steven B.

    2007-03-01

    This paper presents an overview of low energy nuclear reactions, a subset of the field of condensed matter nuclear science. Condensed matter nuclear science studies nuclear effects in and/or on condensed matter, including low energy nuclear reactions, an entirely new branch of science that gained widespread attention and notoriety beginning in 1989 with the announcement of a previously unrecognized source of energy by Martin Fleischmann and Stanley Pons that came to be known as cold fusion. Two branches of LENR are recognized. The first includes a set of reactions like those observed by Fleischmann and Pons that use palladium and deuterium and yield excess heat and helium-4. Numerous mechanisms have been proposed to explain these reactions, however there is no consensus for, or general acceptance of, any of the theories. The claim of fusion is still considered speculative and, as such, is not an ideal term for this work. The other branch is a wide assortment of nuclear reactions that may occur with either hydrogen or deuterium. Anomalous nuclear transmutations are reported that involve light as well as heavy elements. The significant questions that face this field of research are: 1) Are LENRs a genuine nuclear reaction? 2) If so, is there a release of excess energy? 3) If there is, is the energy release cost-effective?

  10. Performance monitoring of low energy house, Macclesfield

    NASA Astrophysics Data System (ADS)

    Stephen, F. R.

    1980-01-01

    The monitoring of the energy balance of a very well insulated low-energy house in Macclesfield, England is discussed. The house is an existing dwelling which had been converted into a low-energy-requiring house by the reduction of heat loss through a high level of thermal insulation and the collection of solar energy by a water cascade solar panel with warm water storage. Measurements of house temperatures, radiation, off-peak electricity consumption and hot water and heating using were performed from January to August, 1978 and reveal that the house used less than 22,000 kWh electricity during that period, compared to 55,000 kWh expected if the house had been constructed to average insulation levels. Solar energy is found to contribute only 2% of house energy requirements, with the use of a heat pump combined with the solar panel leading to greater efficiency and thus utilization. In addition, the large thermal mass and good insulation are found to improve comfort by reducing temperature fluctuations, and the ventilation and low-temperature water return system employed provided satisfactory results.

  11. Low energy cyclotron for radiocarbon dating

    SciTech Connect

    Welch, J.J.

    1984-12-01

    The measurement of naturally occurring radioisotopes whose half lives are less than a few hundred million years but more than a few years provides information about the temporal behavior of geologic and climatic processes, the temporal history of meteoritic bodies as well as the production mechanisms of these radioisotopes. A new extremely sensitive technique for measuring these radioisotopes at tandem Van de Graaff and cyclotron facilities has been very successful though the high cost and limited availability have been discouraging. We have built and tested a low energy cyclotron for radiocarbon dating similar in size to a conventional mass spectrometer. These tests clearly show that with the addition of a conventional ion source, the low energy cyclotron can perform the extremely high sensitivity /sup 14/C measurements that are now done at accelerator facilities. We found that no significant background is present when the cyclotron is tuned to accelerate /sup 14/C negative ions and the transmission efficiency is adequate to perform radiocarbon dating on milligram samples of carbon. The internal ion source used did not produce sufficient current to detect /sup 14/C directly at modern concentrations. We show how a conventional carbon negative ion source, located outside the cyclotron magnet, would produce sufficient beam and provide for quick sampling to make radiocarbon dating milligram samples with a modest laboratory instrument feasible.

  12. 78 FR 58524 - Takes of Marine Mammals Incidental to Specified Activities; U.S. Navy Training and Testing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-24

    ... Study Area AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric... Islands Training and Testing (MITT) Study Area from July 2015 through July 2020. Pursuant to the Marine... incidental to Navy training and testing activities to be conducted in the MITT Study Area over 5 years....

  13. Antifouling activities against colonizer marine bacteria of extracts from marine invertebrates collected in the Colombian Caribbean Sea and on the Brazilian coast (Santa Catarina).

    PubMed

    Mora-Cristancho, Jennyfer A; Arévalo-Ferro, Catalina; Ramos, Freddy A; Tello, Edisson; Duque, Carmenza; Lhullier, Cintia; Falkenberg, Miriam; Schenkel, Eloir Paulo

    2011-01-01

    The growth inhibition of 12 native marine bacteria isolated from Aplysina sponge surfaces, the shell of a bivalve, and Phytagel immersed for 48 h in sea water were used as indicator of the antifouling activity of the extracts of 39 marine organisms (octocorals, sponges, algae, and zoanthid) collected in the Colombian Caribbean Sea and on the Brazilian coast (Santa Catarina). Gram-negative bacteria represented 75% of the isolates; identified strains belonged to Oceanobacillus iheyensis, Ochrobactrum pseudogrignonense, Vibrio campbellii, Vibrio harveyi, and Bacillus megaterium species and seven strains were classified at genus level by the 16S rRNA sequencing method. The extracts of the octocorals Pseudopterogorgia elisabethae, four Eunicea octocorals, and the sponges Topsentia ophiraphidites, Agelas citrina, Neopetrosia carbonaria, Monanchora arbuscula, Cliona tenuis, Iotrochota imminuta, and Ptilocaulis walpersii were the most active, thus suggesting those species as antifoulant producers. This is the first study of natural antifoulants from marine organisms collected on the Colombian and Brazilian coasts.

  14. Comparative Analysis of Glycoside Hydrolases Activities from Phylogenetically Diverse Marine Bacteria of the Genus Arenibacter

    PubMed Central

    Bakunina, Irina; Nedashkovskaya, Olga; Balabanova, Larissa; Zvyagintseva, Tatyana; Rasskasov, Valery; Mikhailov, Valery

    2013-01-01

    A total of 16 marine strains belonging to the genus Arenibacter, recovered from diverse microbial communities associated with various marine habitats and collected from different locations, were evaluated in degradation of natural polysaccharides and chromogenic glycosides. Most strains were affiliated with five recognized species, and some presented three new species within the genus Arenibacter. No strains contained enzymes depolymerizing polysaccharides, but synthesized a wide spectrum of glycosidases. Highly active β-N-acetylglucosaminidases and α-N-acetylgalactosaminidases were the main glycosidases for all Arenibacter. The genes, encoding two new members of glycoside hydrolyses (GH) families, 20 and 109, were isolated and characterized from the genomes of Arenibacter latericius. Molecular genetic analysis using glycosidase-specific primers shows the absence of GH27 and GH36 genes. A sequence comparison with functionally-characterized GH20 and GH109 enzymes shows that both sequences are closest to the enzymes of chitinolytic bacteria Vibrio furnissii and Cellulomonas fimi of marine and terrestrial origin, as well as human pathogen Elisabethkingia meningoseptica and simbionts Akkermansia muciniphila, gut and non-gut Bacteroides, respectively. These results revealed that the genus Arenibacter is a highly taxonomic diverse group of microorganisms, which can participate in degradation of natural polymers in marine environments depending on their niche and habitat adaptations. They are new prospective candidates for biotechnological applications due to their production of unique glycosidases. PMID:23752354

  15. Lesions Arising in a Tattoo of an Active Duty US Marine Corps Woman.

    PubMed

    Winn, Aubrey E; Rivard, Shayna C; Green, Brian

    2016-01-01

    Tattoos are ubiquitous in modern society; however, they do not come without risk of medical complications. When complications arise in the military community, a particularly thorough differential diagnosis should be considered based on the increased exposures service members have during deployment and throughout their military career. We present a case of a 38-year-old active duty US Marine Corps woman with worsening skin lesions arising within a tattoo 6 weeks after acquiring the tattoo on her right chest. Given environmental exposures from a recent deployment to the Middle East, a wide differential was considered. Ultimately, a skin biopsy revealed early hypertrophic scar formation responsive to therapy with intralesional triamcinolone acetonide (Kenalog® [ILK]). However, given the Marine had recently deployed and is part of the active duty population, consideration of alternative, albeit rare, etiologies was imperative. PMID:27450611

  16. Low Energy Antiproton Experiments - A Review

    SciTech Connect

    Jungmann, Klaus P.

    2005-10-19

    Low energy antiprotons offer excellent opportunities to study properties of fundamental forces and symmetries in nature. Experiments with them can contribute substantially to deepen our fundamental knowledge in atomic, nuclear and particle physics. Searches for new interactions can be carried out by studying discrete symmetries. Known interactions can be tested precisely and fundamental constants can be extracted from accurate measurements on free antiprotons (p-bar's) and bound two- and three-body systems such as antihydrogen (H-bar = p-bare-), the antprotonic helium ion (He++p-bar)+ and the antiprotonic atomcule (He++p-bare-) . The trapping of a single p-bar in a Penning trap, the formation and precise studies of antiprotonic helium ions and atoms and recently the production of H-bar have been among the pioneering experiments. They have led already to precise values for p-bar parameters, accurate tests of bound two- and three-body Quantum Electrodynamics (QED), tests of the CPT theorem and a better understanding of atom formation from their constituents. Future experiments promise more precise tests of the standard theory and have a robust potential to discover new physics. Precision experiments with low energy p-bar's share the need for intense particle sources and the need for time to develop novel instrumentation with all other experiments, which aim for high precision in exotic fundamental systems. The experimental programs - carried out in the past mostly at the former LEAR facility and at present at the AD facility at CERN - would benefit from intense future sources of low energy p-bar's. The highest possible p-bar fluxes should be aimed for at new facilities such as the planned FLAIR facility at GSI in order to maximize the potential of delicate precision experiments to influence model building. Examples of key p-bar experiments are discussed here and compared with other experiments in the field. Among the central issues is their potential to obtain

  17. 78 FR 19224 - Taking of Marine Mammals Incidental to Specified Activities; U.S. Marine Corps Training Exercises...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    ... Marine Fisheries Service, National Oceanic and Atmospheric Administration (NOAA), Commerce. ACTION..., ``Environmental Assessment MCAS Cherry Point Range Operations,'' for their federal action of supporting and... information on the scope of our federal action and resultant environmental impacts for purposes of...

  18. An efficient synthesis method targeted to marine alkaloids marinacarbolines A-D and their antitumor activities.

    PubMed

    Li, Jun; Tang, Yang; Jin, Hui-Juan; Cui, Yi-Di; Zhang, Li-Juan; Jiang, Tao

    2015-01-01

    Marinacarbolines A-D are a series of marine β-carboline alkaloids isolated from actinomycete Marinactinospora thermotolerans of the deep South China Sea with antiplasmodial activities. In inhibition assays of in vitro growth of Plasmodium falciparum, marinacarbolines exhibited antiplasmodial activity against drug-sensitive line 3D7 and drug-resistant line Dd2 of P. falciparum. However, approaches for the synthesis of such useful compounds are very limited. In this work, we reported a simple, efficient, and versatile process to synthesize marinacarbolines A-D (1-4). On the basis of that, the antitumor activities of marinacarbolines in a structure-dependent manner were allowed to be unveiled.

  19. Cytotoxic and HIV-1 enzyme inhibitory activities of Red Sea marine organisms

    PubMed Central

    2014-01-01

    Background Cancer and HIV/AIDS are two of the greatest public health and humanitarian challenges facing the world today. Infection with HIV not only weakens the immune system leading to AIDS and increasing the risk of opportunistic infections, but also increases the risk of several types of cancer. The enormous biodiversity of marine habitats is mirrored by the molecular diversity of secondary metabolites found in marine animals, plants and microbes which is why this work was designed to assess the anti-HIV and cytotoxic activities of some marine organisms of the Red Sea. Methods The lipophilic fractions of methanolic extracts of thirteen marine organisms collected from the Red Sea (Egypt) were screened for cytotoxicity against two human cancer cell lines; leukaemia (U937) and cervical cancer (HeLa) cells. African green monkey kidney cells (Vero) were used as normal non-malignant control cells. The extracts were also tested for their inhibitory activity against HIV-1 enzymes, reverse transcriptase (RT) and protease (PR). Results Cytotoxicity results showed strong activity of the Cnidarian Litophyton arboreum against U-937 (IC50; 6.5 μg/ml ±2.3) with a selectivity index (SI) of 6.45, while the Cnidarian Sarcophyton trochliophorum showed strong activity against HeLa cells (IC50; 5.2 μg/ml ±1.2) with an SI of 2.09. Other species showed moderate to weak cytotoxicity against both cell lines. Two extracts showed potent inhibitory activity against HIV-1 protease; these were the Cnidarian jelly fish Cassiopia andromeda (IC50; 0.84 μg/ml ±0.05) and the red algae Galaxura filamentosa (2.6 μg/ml ±1.29). It is interesting to note that the most active extracts against HIV-1 PR, C. andromeda and G. filamentosa showed no cytotoxicity in the three cell lines at the highest concentration tested (100 μg/ml). Conclusion The strong cytotoxicity of the soft corals L. arboreum and S. trochliophorum as well as the anti-PR activity of the jelly fish C. andromeda and the red

  20. Phenomenological implications of low energy supersymmetry breaking

    SciTech Connect

    Dimopoulos, S. |; Dine, M.; Raby, S.; Thomas, S.; Wells, J.D.

    1996-07-01

    The experimental signatures for low energy supersymmetry breaking are presented. The lightest standard model superpartner is unstable and decays to its partner plus a Goldstino, G. For a supersymmetry breaking scale below a few 1,000 TeV this decay can take place within a detector, leading to very distinctive signatures. If a neutralino is the lightest standard model superpartner it decays by {chi}{sub 1}{sup 0} {r_arrow} {gamma} + G, and if kinematically accessible by {chi}{sub 1}{sup 0} {r_arrow} (Z{sup 0}, h{sup 0}, H{sup 0}, A{sup 0}) + G. These decays can give rise to displaced vertices. Alternately, if a slepton is the lightest standard model superpartner it decays by {tilde l} {r_arrow} l + G. This can be seen as a greater than minimum ionizing charged particle track, possibly with a kink to a minimum ionizing track.

  1. Low energy ion-molecule reactions

    SciTech Connect

    Farrar, J.M.

    1993-12-01

    This project is concerned with elucidating the dynamics of elementary ion-molecule reactions at collision energies near and below 1 eV. From measurements of the angular and energy distributions of the reaction products, one can infer intimathe details about the nature of collisions leading to chemical reaction, the geometries and lifetimes of intermediate complexes that govern the reaction dynamics, and the collision energy dependence of these dynamical features. The author employs crossed-beam low energy mass spectrometry technology developed over the last several years, with the focus of current research on proton transfer and hydrogen atom transfer reactions of te O{sup {minus}} ion with species such as HF, H{sub 2}O, and NH{sub 3}.

  2. Low energy high pressure miniature screw valve

    DOEpatents

    Fischer, Gary J.; Spletzer, Barry L.

    2006-12-12

    A low energy high pressure screw valve having a valve body having an upper portion and a lower portion, said lower portion of said valve body defining an inlet flow passage and an outlet flow passage traversing said valve body to a valve seat, said upper portion of said valve body defining a cavity at said valve seat, a diaphragm restricting flow between said upper portion of said valve body and said lower portion, said diaphragm capable of engaging said valve seat to restrict fluid communication between said inlet passage and said outlet passage, a plunger within said cavity supporting said diaphragm, said plunger being capable of engaging said diaphragm with said valve seat at said inlet and outlet fluid passages, said plunger being in point contact with a drive screw having threads engaged with opposing threads within said upper portion of said valve body such engagement allowing motion of said drive screw within said valve body.

  3. Low-energy irradiation effects in cellulose

    SciTech Connect

    Polvi, Jussi; Nordlund, Kai

    2014-01-14

    Using molecular dynamics simulations, we determined the threshold energy for creating defects as a function of the incident angle for all carbon and oxygen atoms in the cellulose monomer. Our analysis shows that the damage threshold energy is strongly dependent on the initial recoil direction and on average slightly higher for oxygen atoms than for carbon atoms in cellulose chain. We also performed cumulative bombardment simulations mimicking low-energy electron irradiation (such as TEM imaging) on cellulose. Analyzing the results, we found that formation of free molecules and broken glucose rings were the most common forms of damage, whereas cross-linking and chain scission were less common. Pre-existing damage was found to increase the probability of cross-linking.

  4. Low-energy neutral-atom spectrometer

    SciTech Connect

    Voss, D.E.; Cohen, S.A.

    1982-04-01

    The design, calibration, and performance of a low energy neutral atom spectrometer are described. Time-of-flight analysis is used to measure the energy spectrum of charge-exchange deuterium atoms emitted from the PLT tokamak plasma in the energy range from 20 to 1000 eV. The neutral outflux is gated on a 1 ..mu..sec time scale by a slotted rotating chopper disc, supported against gravity in vacuum by magnetic levitation, and is detected by secondary electron emission from a Cu-Be plate. The energy dependent detection efficiency has been measured in particle beam experiments and on the tokamak so that the diagnostic is absolutely calibrated, allowing quantitative particle fluxes to be determined with 200 ..mu..sec time resolution. In addition to its present application as a plasma diagnostic, the instrument is capable of making a wide variety of measurements relevant to atomic and surface physics.

  5. RHIC low energy tests and initial operations

    SciTech Connect

    Satogata,T.; Ahrens, L.; Bai, M.; Brennan, J.M.; Bruno, D.; Butler, J.; Drees, A.; Fedotov, A.; Fischer, W.; Harvey, M.; Hayes, T.; Jappe, W.; Lee, R.C.; Mackay, W.W.; Malitsky, N.; Marr, G.; Michnoff, R.; Oerter, B.; Pozdeyev, E.; Roser, T.; Severino, F.; Smith, K.; Tepikian, S.; Tsoupas, N.

    2009-05-04

    Future Relativistic Heavy Ion Collider (RHIC) runs, including a portion of FY10 heavy ion operations, will explore collisions at center of mass energies of 5-50 GeV/n (GeV/nucleon). Operations at these energies is motivated by a search for the QCD phase transition critical point. The lowest end of this energy range is nearly a factor of four below the nominal RHIC injection center of mass energy of {radical} s = 20.8 GeV/n. There are several operational challenges in the RHIC low-energy regime, including harmonic number changes, small longitudinal acceptance, lowered magnet field quality, nonlinear orbit control, and luminosity monitoring. We report on the experience with some of these challenges during beam tests with gold in March 2008, including first RHIC operations at {radical}s = 9.18 GeV/n and first beam experience at {radical}s = 5 GeV/n.

  6. Detection of low energy antimatter with emulsions

    NASA Astrophysics Data System (ADS)

    Aghion, S.; Ariga, A.; Ariga, T.; Bollani, M.; Dei Cas, E.; Ereditato, A.; Evans, C.; Ferragut, R.; Giammarchi, M.; Pistillo, C.; Romé, M.; Sala, S.; Scampoli, P.

    2016-06-01

    Emulsion detectors feature a very high position resolution and consequently represent an ideal device when particle detection is required at the micrometric scale. This is the case of quantum interferometry studies with antimatter, where micrometric fringes have to be measured. In this framework, we designed and realized a new emulsion based detector characterized by a gel enriched in terms of silver bromide crystal contents poured on a glass plate. We tested the sensitivity of such a detector to low energy positrons in the range 10–20 keV . The obtained results prove that nuclear emulsions are highly efficient at detecting positrons at these energies. This achievement paves the way to perform matter-wave interferometry with positrons using this technology.

  7. Low energy consumption spintronics using multiferroic heterostructures.

    PubMed

    Trassin, Morgan

    2016-01-27

    We review the recent progress in the field of multiferroic magnetoelectric heterostructures. The lack of single phase multiferroic candidates exhibiting simultaneously strong and coupled magnetic and ferroelectric orders led to an increased effort into the development of artificial multiferroic heterostructures in which these orders are combined by assembling different materials. The magnetoelectric coupling emerging from the created interface between the ferroelectric and ferromagnetic layers can result in electrically tunable magnetic transition temperature, magnetic anisotropy or magnetization reversal. The full potential of low energy consumption magnetic based devices for spintronics lies in our understanding of the magnetoelectric coupling at the scale of the ferroic domains. Although the thin film synthesis progresses resulted into the complete control of ferroic domain ordering using epitaxial strain, the local observation of magnetoelectric coupling remains challenging. The ability to imprint ferroelectric domains into ferromagnets and to manipulate those solely using electric fields suggests new technological advances for spintronics such as magnetoelectric memories or memristors.

  8. The Superconducting Super Collider Low Energy Booster

    SciTech Connect

    York, R.C.; Funk, W.; Garren, A.; Machida, S.; Mahale, N.K.; Peterson, J.; Pilat, F.; Wu, X. ); Wienands, U. )

    1991-05-01

    In collider fill mode, the Low Energy Booster (LEB) will accelerate 10{sup 12} protons in 114 bunches from an injection momentum of 1.22 GeV/c to a final momentum of 12 Gev/c, cycling at a frequency of 10 Hz. The most significant extension of present fast-cycling synchrotron technology arises from the requirement that the normalized transverse emittance (rms) of the beam be {le}0.6 {pi} {mu}m. In an alternative mode, the LEB will accelerate five times this current with a normalized transverse emittance {le} 4 {pi} {mu}m. A general overview of the design is presented. 7 refs., 2 figs., 4 tabs.

  9. Low-energy pion-nucleon scattering

    SciTech Connect

    Gibbs, W.R.; Ai, L.; Kaufmann, W.B.

    1998-02-01

    An analysis of low-energy charged pion-nucleon data from recent {pi}{sup {plus_minus}}p experiments is presented. From the scattering lengths and the Goldberger-Miyazawa-Oehme (GMO) sum rule we find a value of the pion-nucleon coupling constant of f{sup 2}=0.0756{plus_minus}0.0007. We also find, contrary to most previous analyses, that the scattering volumes for the P{sub 31} and P{sub 13} partial waves are equal, within errors, corresponding to a symmetry found in the Hamiltonian of many theories. For the potential models used, the amplitudes are extrapolated into the subthreshold region to estimate the value of the {Sigma} term. Off-shell amplitudes are also provided. {copyright} {ital 1998} {ital The American Physical Society}

  10. Low-energy pion-nucleon scattering

    NASA Astrophysics Data System (ADS)

    Gibbs, W. R.; Ai, Li; Kaufmann, W. B.

    1998-02-01

    An analysis of low-energy charged pion-nucleon data from recent π+/-p experiments is presented. From the scattering lengths and the Goldberger-Miyazawa-Oehme (GMO) sum rule we find a value of the pion-nucleon coupling constant of f2=0.0756+/-0.0007. We also find, contrary to most previous analyses, that the scattering volumes for the P31 and P13 partial waves are equal, within errors, corresponding to a symmetry found in the Hamiltonian of many theories. For the potential models used, the amplitudes are extrapolated into the subthreshold region to estimate the value of the Σ term. Off-shell amplitudes are also provided.

  11. Survival and activity of Streptococcus faecalis and Escherichia coli in petroleum-contaminated tropical marine waters

    SciTech Connect

    Santo Domingo, J.W.; Fuentes, F.A.; Hazen, T.C.

    1987-12-31

    The in situ survival and activity of Streptococcus faecalis and Escherichia coli were studied using membrane diffusion chambers in tropical marine waters receiving oil refinery effluents. Protein synthesis, DNA synthesis, respiration or fermentation, INT reduced per cell, and ATP per cell were used to measure physiological activity. Cell densities decreased significantly over time at both sites for both S. faecalis and E. coli; however, no significant differences in survival pattern were observed between S. faecalis and E.coli. Differences in protein synthesis between the two were only observed at a study site which was not heavily oiled. Although fecal streptococci have been suggested as a better indicator of fecal contamination than fecal coliforms in marine waters, in this study both E. coli and S. faecalis survived and remained physiologically active for extended periods of time. These results suggest that the fecal streptococci group is not a better indicator of fecal contamination in tropical marine waters than the fecal coliform group, especially when that environment is high in long-chained hydrocarbons.

  12. Low energy AMS of americium and curium

    NASA Astrophysics Data System (ADS)

    Christl, Marcus; Dai, Xiongxin; Lachner, Johannes; Kramer-Tremblay, Sheila; Synal, Hans-Arno

    2014-07-01

    Accelerator mass spectrometry (AMS) has evolved over the past years as one of the most sensitive, selective, and robust techniques for actinide analyses. While analyses of U and Pu isotopes have already become routine at the ETH Zurich 0.5 MV AMS system "Tandy", there is an increasing demand for highly sensitive analyses of the higher actinides such as Am and Cm for bioassay applications and beyond. In order to extend the actinide capabilities of the compact ETH Zurich AMS system and to develop new, more sensitive bioassay routines, a pilot study was carried out. The aim was to investigate and document the performance and the potential background of Am and Cm analyses with low energy AMS. Our results show that 241Am and Cm isotopes can be determined relative to a 243Am tracer if samples and AMS standards are prepared identically with regard to the matrix elements, in which the sample is dispersed. In this first test, detection limits for Cm and Am isotopes are all in the sub-femtogram range and even below 100 ag for Cm isotopes. In a systematic background study in the mass range of the Cm isotopes, two formerly unknown metastable triply charged Th molecules were found on amu(244) and amu(248). The presence of such a background is not a principal problem for AMS if the stripper pressure is increased accordingly. Based on our first results, we conclude that ultra-trace analyses of Am and Cm isotopes for bioassay are very well possible with low energy AMS.

  13. Low energy demonstration accelerator technical area 53

    SciTech Connect

    1996-04-01

    As part of the Department of Energy`s (DOE) need to maintain the capability of producing tritium in support of its historic and near-term stewardship of the nation`s nuclear weapons stockpile, the agency has recently completed a Programmatic Environmental Impact Statement for Tritium Supply and Recycling. The resulting Record of Decision (ROD) determined that over the next three years the DOE would follow a dual-track acquisition strategy that assures tritium production for the nuclear weapon stockpile in a rapid, cost effective, and safe manner. Under this strategy the DOE will further investigate and compare two options for producing tritium: (1) purchase of an existing commercial light-water reactor or irradiation services with an option to purchase the reactor for conversion to a defense facility; and (2) design, build, and test critical components of a system for accelerator production of tritium (APT). The final decision to select the primary production option will be made by the Secretary of Energy in the October 1998 time frame. The alternative not chosen as the primary production method, if feasible, would be developed as a back-up tritium supply source. This Environmental Assessment (EA) analyzes the potential environmental effects that would be expected to occur if the DOE were to design, build, and test critical prototypical components of the accelerator system for tritium production, specifically the front-end low-energy section of the accelerator, at Los Alamos National Laboratory. The Low Energy Demonstration Accelerator (LEDA) would be incrementally developed and tested in five separate stages over the next seven years. The following issues were evaluated for the proposed action: utility demands, air, human health, environmental restoration, waste management, transportation, water, threatened and endangered species, wetlands, cultural resources, and environmental justice.

  14. The Low Energy Effective Area of the Chandra Low Energy Transmission Grating Spectrograph

    NASA Technical Reports Server (NTRS)

    Pease, D.; Drake, J. J.; Johnson, C. O.; Kashya, V.; Ratzlaff, P. W.; Wargelin, B. J.; Brinkman, A. C.; Kaastra, J. S.; vanderMeer, R.; Paerels, F. B.

    2000-01-01

    The Chandra X-ray Observatory was successfully launched on July 23, 1999, and subsequently began an intensive calibration phase. We present the preliminary results from the in-flight calibration of the low energy response of the High Resolution Camera spectroscopic readout (HRC-S) combined with the Low Energy Transmission Grating (LETG) aboard Chandra. These instruments comprise the Low Energy Transmission Grating Spectrograph (LETGS). For this calibration study, we employ a pure hydrogen non-LTE white dwarf emission model (T = 25000 K and log g = 9.0) for comparison with the Chandra observations of Sirius B. The pre-flight calibration of the LETGS effective area only covered wavelengths shortward of 44 A (E less than 277 eV). Our Sirius B analysis shows that the HRC-S quantum efficiency (QE) model assumed for longer wavelengths leads to an overestimate of the effective area by an average factor of about 1.6. We derive a correction to the low energy HRC-S QE model to match the predicted and observed Sirius B spectra over the wavelength range of 44-185 A. We make an independent test of our results by the comparison of a Chandra LETGS observation of HZ 43 with pure hydrogen model atmosphere predictions and find good agreement.

  15. 21 CFR 878.4410 - Low energy ultrasound wound cleaner.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Low energy ultrasound wound cleaner. 878.4410... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4410 Low energy ultrasound wound cleaner. (a) Identification. A low energy ultrasound wound cleaner is a device that...

  16. 21 CFR 878.4410 - Low energy ultrasound wound cleaner.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Low energy ultrasound wound cleaner. 878.4410... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4410 Low energy ultrasound wound cleaner. (a) Identification. A low energy ultrasound wound cleaner is a device that...

  17. 21 CFR 878.4410 - Low energy ultrasound wound cleaner.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Low energy ultrasound wound cleaner. 878.4410... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4410 Low energy ultrasound wound cleaner. (a) Identification. A low energy ultrasound wound cleaner is a device that...

  18. 21 CFR 878.4410 - Low energy ultrasound wound cleaner.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Low energy ultrasound wound cleaner. 878.4410... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4410 Low energy ultrasound wound cleaner. (a) Identification. A low energy ultrasound wound cleaner is a device that...

  19. 21 CFR 878.4410 - Low energy ultrasound wound cleaner.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Low energy ultrasound wound cleaner. 878.4410... (CONTINUED) MEDICAL DEVICES GENERAL AND PLASTIC SURGERY DEVICES Surgical Devices § 878.4410 Low energy ultrasound wound cleaner. (a) Identification. A low energy ultrasound wound cleaner is a device that...

  20. Low energy electrons in the inner Earth's magnetosphere

    NASA Astrophysics Data System (ADS)

    Ganushkina, Natalia; Sillanpaa, Ilkka; Dugyagin, Stepan; Pitchford, David; Rodriguez, Juan; Runov, Andrei

    2016-04-01

    The fluxes of electrons with energies < 100 keV are not usually analyzed and modeled in details when studying the electron radiation belts. These fluxes constitute the low energy part of the seed population, which is critically important for radiation belt dynamics. Moreover, energetic electrons with energies less than about 100 keV are responsible for hazardous space-weather phenomena such as surface charging. The electron flux at these energies varies highly with geomagnetic activity and even during quiet-time periods. Significant variations in the low-energy electrons can be seen during isolated substorms, not related to any storm periods. Moreover, electron flux variations depend on the electron energy. Statistical analysis of AMC 12 CEASE II ESA instrument data (5-50 keV) and GOES MAGED data (40, 75, 150 keV) have revealed that electron fluxes increase by the same order of magnitude during isolated substorms with 200 nT of AE index and storm-time substorms with 1200 nT of AE index. If substorms are represented as electromagnetic pulses which transport and accelerate electrons additionally, how are their amplitudes determined, if not related directly to a substorm's strength? Another factor of crucial importance is the specification of boundary conditions in the electron plasma sheet. We developed a new model for electron number density and temperature in the plasma sheet as dependent on solar wind and IMF conditions based on THEMIS data analysis. We present observational and modeling results on low energy electrons in the inner magnetosphere with newly-developed, time-dependent boundary conditions with a special focus on the role of substorms for electron transport and acceleration.

  1. Membrane-active peptides from marine organisms--antimicrobials, cell-penetrating peptides and peptide toxins: applications and prospects.

    PubMed

    Ponnappan, Nisha; Budagavi, Deepthi Poornima; Yadav, Bhoopesh Kumar; Chugh, Archana

    2015-03-01

    Marine organisms are known to be a rich and unique source of bioactive compounds as they are exposed to extreme conditions in the oceans. The present study is an attempt to briefly describe some of the important membrane-active peptides (MAPs) such as antimicrobial peptides (AMPs), cell-penetrating peptides (CPPs) and peptide toxins from marine organisms. Since both AMPs and CPPs play a role in membrane perturbation and exhibit interchangeable role, they can speculatively fall under the broad umbrella of MAPs. The study focuses on the structural and functional characteristics of different classes of marine MAPs. Further, AMPs are considered as a potential remedy to antibiotic resistance acquired by several pathogens. Peptides from marine organisms show novel post-translational modifications such as cysteine knots, halogenation and histidino-alanine bridge that enable these peptides to withstand harsh marine environmental conditions. These unusual modifications of AMPs from marine organisms are expected to increase their half-life in living systems, contributing to their increased bioavailability and stability when administered as drug in in vivo systems. Apart from AMPs, marine toxins with membrane-perturbing properties could be essentially investigated for their cytotoxic effect on various pathogens and their cell-penetrating activity across various mammalian cells. The current review will help in identifying the MAPs from marine organisms with crucial post-translational modifications that can be used as template for designing novel therapeutic agents and drug-delivery vehicles for treatment of human diseases.

  2. The marine sponge-derived polyketide endoperoxide plakortide F acid mediates its antifungal activity by interfering with calcium homeostasis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plakortide F acid (PFA) is a marine-derived polyketide endoperoxide exhibiting strong inhibitory activity against several clinically important fungal pathogens. In the present study, transcriptional profiling coupled with mutant and biochemical analyses were conducted using the model organism Sacch...

  3. Effects of Pharmaceuticals Used for Breast Cancer Treatment on Reproduction and Aromatase Activity in a Marine Fish

    EPA Science Inventory

    Laboratory experiments were conducted with the marine fish cunner (Tautogolabrus adspersus) to evaluate whether four pharmaceuticals used in breast cancer treatment have an impact on reproduction or aromatase activity. Tamoxifen binds to estrogen receptors, while anastrozole, let...

  4. [Low-energy laser therapy in medial tibial stress syndrome].

    PubMed

    Nissen, L R; Astvad, K; Madsen, L

    1994-12-01

    The effect of low-energy laser therapy on shin splints was examined in a randomized study with an unblinded design. Constripts from the Jutland Dragoon regiment with shin splints were given either active laser treatment (40 mW in 60 sec per cm tender tibia edge) or placebo laser. All patients were exempted from normal duty concerning activities like running and march. Forty-nine patients participated in the study, 23 in the laser group and 26 in the control group. From the start the study was designed to be double-blind, but by accident the code was broken towards the end of the study. We found no significant differences between the groups regarding pain visual analog score and readiness to return to active duty after 14 days.

  5. Status report on the Low Energy Neutron Source for 2015

    NASA Astrophysics Data System (ADS)

    Baxter, D. V.; Rinckel, T.

    2016-11-01

    The Low Energy Neutron Source at Indiana University first produced cold neutrons in April of 2005. Ten years after first reaching this milestone, the facility has three instruments in operation on its cold target station, and a second target station is devoted to thermal and fast neutron physics offers capabilities in radiation effects research (single-event effects in electronics) and radiography. Key elements in our success over these last ten years have been the diversity of activities we have been able maintain (which often involves using each of our instruments for multiple different activities), the close relationship we have developed with a number of major sources, and the focus we have had on innovation in neutron instrumentation. In this presentation, we will introduce some of the highlights from our most recent activities, provide an update on some of our technical challenges, and describe some of our ideas for the future.

  6. In vitro evaluation of marine-microorganism extracts for anti-viral activity

    PubMed Central

    2010-01-01

    Viral-induced infectious diseases represent a major health threat and their control remains an unachieved goal, due in part to the limited availability of effective anti-viral drugs and measures. The use of natural products in drug manufacturing is an ancient and well-established practice. Marine organisms are known producers of pharmacological and anti-viral agents. In this study, a total of 20 extracts from marine microorganisms were evaluated for their antiviral activity. These extracts were tested against two mammalian viruses, herpes simplex virus (HSV-1) and vesicular stomatitis virus (VSV), using Vero cells as the cell culture system, and two marine virus counterparts, channel catfish virus (CCV) and snakehead rhabdovirus (SHRV), in their respective cell cultures (CCO and EPC). Evaluation of these extracts demonstrated that some possess antiviral potential. In sum, extracts 162M(4), 258M(1), 298M(4), 313(2), 331M(2), 367M(1) and 397(1) appear to be effective broad-spectrum antivirals with potential uses as prophylactic agents to prevent infection, as evident by their highly inhibitive effects against both virus types. Extract 313(2) shows the most potential in that it showed significantly high inhibition across all tested viruses. The samples tested in this study were crude extracts; therefore the development of antiviral application of the few potential extracts is dependent on future studies focused on the isolation of the active elements contained in these extracts. PMID:20691099

  7. The antimicrobial activity of heterotrophic bacteria isolated from the marine sponge Erylus deficiens (Astrophorida, Geodiidae)

    PubMed Central

    Graça, Ana Patrícia; Viana, Flávia; Bondoso, Joana; Correia, Maria Inês; Gomes, Luis; Humanes, Madalena; Reis, Alberto; Xavier, Joana R.; Gaspar, Helena; Lage, Olga M.

    2015-01-01

    Interest in the study of marine sponges and their associated microbiome has increased both for ecological reasons and for their great biotechnological potential. In this work, heterotrophic bacteria associated with three specimens of the marine sponge Erylus deficiens, were isolated in pure culture, phylogenetically identified and screened for antimicrobial activity. The isolation of bacteria after an enrichment treatment in heterotrophic medium revealed diversity in bacterial composition with only Pseudoalteromonas being shared by two specimens. Of the 83 selected isolates, 58% belong to Proteobacteria, 23% to Actinobacteria and 19% to Firmicutes. Diffusion agar assays for bioactivity screening against four bacterial strains and one yeast, revealed that a high number of the isolated bacteria (68.7%) were active, particularly against Candida albicans and Vibrio anguillarum. Pseudoalteromonas, Microbacterium, and Proteus were the most bioactive genera. After this preliminary screening, the bioactive strains were further evaluated in liquid assays against C. albicans, Bacillus subtilis and Escherichia coli. Filtered culture medium and acetone extracts from three and 5 days-old cultures were assayed. High antifungal activity against C. albicans in both aqueous and acetone extracts as well as absence of activity against B. subtilis were confirmed. Higher levels of activity were obtained with the aqueous extracts when compared to the acetone extracts and differences were also observed between the 3 and 5 day-old extracts. Furthermore, a low number of active strains was observed against E. coli. Potential presence of type-I polyketide synthases (PKS-I) and non-ribosomal peptide synthetases (NRPSs) genes were detected in 17 and 30 isolates, respectively. The high levels of bioactivity and the likely presence of associated genes suggest that Erylus deficiens bacteria are potential sources of novel marine bioactive compounds. PMID:25999928

  8. The antimicrobial activity of heterotrophic bacteria isolated from the marine sponge Erylus deficiens (Astrophorida, Geodiidae).

    PubMed

    Graça, Ana Patrícia; Viana, Flávia; Bondoso, Joana; Correia, Maria Inês; Gomes, Luis; Humanes, Madalena; Reis, Alberto; Xavier, Joana R; Gaspar, Helena; Lage, Olga M

    2015-01-01

    Interest in the study of marine sponges and their associated microbiome has increased both for ecological reasons and for their great biotechnological potential. In this work, heterotrophic bacteria associated with three specimens of the marine sponge Erylus deficiens, were isolated in pure culture, phylogenetically identified and screened for antimicrobial activity. The isolation of bacteria after an enrichment treatment in heterotrophic medium revealed diversity in bacterial composition with only Pseudoalteromonas being shared by two specimens. Of the 83 selected isolates, 58% belong to Proteobacteria, 23% to Actinobacteria and 19% to Firmicutes. Diffusion agar assays for bioactivity screening against four bacterial strains and one yeast, revealed that a high number of the isolated bacteria (68.7%) were active, particularly against Candida albicans and Vibrio anguillarum. Pseudoalteromonas, Microbacterium, and Proteus were the most bioactive genera. After this preliminary screening, the bioactive strains were further evaluated in liquid assays against C. albicans, Bacillus subtilis and Escherichia coli. Filtered culture medium and acetone extracts from three and 5 days-old cultures were assayed. High antifungal activity against C. albicans in both aqueous and acetone extracts as well as absence of activity against B. subtilis were confirmed. Higher levels of activity were obtained with the aqueous extracts when compared to the acetone extracts and differences were also observed between the 3 and 5 day-old extracts. Furthermore, a low number of active strains was observed against E. coli. Potential presence of type-I polyketide synthases (PKS-I) and non-ribosomal peptide synthetases (NRPSs) genes were detected in 17 and 30 isolates, respectively. The high levels of bioactivity and the likely presence of associated genes suggest that Erylus deficiens bacteria are potential sources of novel marine bioactive compounds.

  9. Marine pharmacology in 2007-8: Marine compounds with antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous system, and other miscellaneous mechanisms of action.

    PubMed

    Mayer, Alejandro M S; Rodríguez, Abimael D; Berlinck, Roberto G S; Fusetani, Nobuhiro

    2011-03-01

    The peer-reviewed marine pharmacology literature in 2007-8 is covered in this review, which follows a similar format to the previous 1998-2006 reviews of this series. The preclinical pharmacology of structurally characterized marine compounds isolated from marine animals, algae, fungi and bacteria is discussed in a comprehensive manner. Antibacterial, anticoagulant, antifungal, antimalarial, antiprotozoal, antituberculosis and antiviral activities were reported for 74 marine natural products. Additionally, 59 marine compounds were reported to affect the cardiovascular, immune and nervous systems as well as to possess anti-inflammatory effects. Finally, 65 marine metabolites were shown to bind to a variety of receptors and miscellaneous molecular targets, and thus upon further completion of mechanism of action studies, will contribute to several pharmacological classes. Marine pharmacology research during 2007-8 remained a global enterprise, with researchers from 26 countries, and the United States, contributing to the preclinical pharmacology of 197 marine compounds which are part of the preclinical marine pharmaceuticals pipeline. Sustained preclinical research with marine natural products demonstrating novel pharmacological activities, will probably result in the expansion of the current marine pharmaceutical clinical pipeline, which currently consists of 13 marine natural products, analogs or derivatives targeting a limited number of disease categories.

  10. Viewing Marine Bacteria, Their Activity and Response to Environmental Drivers from Orbit

    PubMed Central

    Grimes, D. Jay; Ford, Tim E.; Colwell, Rita R.; Baker-Austin, Craig; Martinez-Urtaza, Jaime; Subramaniam, Ajit; Capone, Douglas G.

    2014-01-01

    Satellite-based remote sensing of marine microorganisms has become a useful tool in predicting human health risks associated with these microscopic targets. Early applications were focused on harmful algal blooms, but more recently methods have been developed to interrogate the ocean for bacteria. As satellite-based sensors have become more sophisticated and our ability to interpret information derived from these sensors has advanced, we have progressed from merely making fascinating pictures from space to developing process models with predictive capability. Our understanding of the role of marine microorganisms in primary production and global elemental cycles has been vastly improved as has our ability to use the combination of remote sensing data and models to provide early warning systems for disease outbreaks. This manuscript will discuss current approaches to monitoring cyanobacteria and vibrios, their activity and response to environmental drivers, and will also suggest future directions. PMID:24477922

  11. The Sound of Silence: Activating Silent Biosynthetic Gene Clusters in Marine Microorganisms

    PubMed Central

    Reen, F. Jerry; Romano, Stefano; Dobson, Alan D.W.; O’Gara, Fergal

    2015-01-01

    Unlocking the rich harvest of marine microbial ecosystems has the potential to both safeguard the existence of our species for the future, while also presenting significant lifestyle benefits for commercial gain. However, while significant advances have been made in the field of marine biodiscovery, leading to the introduction of new classes of therapeutics for clinical medicine, cosmetics and industrial products, much of what this natural ecosystem has to offer is locked in, and essentially hidden from our screening methods. Releasing this silent potential represents a significant technological challenge, the key to which is a comprehensive understanding of what controls these systems. Heterologous expression systems have been successful in awakening a number of these cryptic marine biosynthetic gene clusters (BGCs). However, this approach is limited by the typically large size of the encoding sequences. More recently, focus has shifted to the regulatory proteins associated with each BGC, many of which are signal responsive raising the possibility of exogenous activation. Abundant among these are the LysR-type family of transcriptional regulators, which are known to control production of microbial aromatic systems. Although the environmental signals that activate these regulatory systems remain unknown, it offers the exciting possibility of evoking mimic molecules and synthetic expression systems to drive production of potentially novel natural products in microorganisms. Success in this field has the potential to provide a quantum leap forward in medical and industrial bio-product development. To achieve these new endpoints, it is clear that the integrated efforts of bioinformaticians and natural product chemists will be required as we strive to uncover new and potentially unique structures from silent or cryptic marine gene clusters. PMID:26264003

  12. Equal Opportunities for Women in Marine Sciences in Kiel: Activities and Measures

    NASA Astrophysics Data System (ADS)

    Kamm, Ruth

    2016-04-01

    Women are still largely underrepresented in geosciences in general. Particularly at the level of professorships and permanent research staff positions this also applies to marine science institutions in Kiel, i.e. the research focus Kiel Marine Sciences at Kiel University and the GEOMAR Helmholtz Centre for Ocean Research Kiel. Both institutions are closely collaborating, for instance in the frame of two major third-party funded collaborative projects: The Cluster of Excellence 'The Future Ocean', funded within the German Excellence Initiative, and the Collaborative Research Centre 'Climate - Biogeochemistry Interactions in the Tropical Ocean' (SFB 754) financed through the German Research Foundation (DFG). Both funding schemes request for measures to increase the participation of female scientists in leading positions. As an innovative approach, The Future Ocean and SFB 754 jointly finance the position of a coordinator for gender measures who is based at the university's Central Office for Gender Equality, Diversity & Family since 2012. This allows for the coordinated development and implementation of programmes to support female marine scientists, with a focus on the postdoctoral phase, and to offer a broader spectrum of activities to raise awareness of gender imbalance in the research community. The aim of this presentation is to give insight into activities and achievements, among them the mentoring programme via:mento_ocean for female postdocs in marine sciences. The programme via:mento_ocean has been acknowledged as a best practice instrument to support women scientists in a close disciplinary but international setting and was incorporated into the DFG's online toolbox of gender equality measures.

  13. Cell cycle arrest and activation of development in marine invertebrate deuterostomes.

    PubMed

    Costache, Vlad; McDougall, Alex; Dumollard, Rémi

    2014-08-01

    Like most metazoans, eggs of echinoderms and tunicates (marine deuterostomes, there is no data for the cephalochordates) arrest awaiting fertilization due to the activity of the Mos/MEK/MAPK cascade and are released from this cell cycle arrest by sperm-triggered Ca2+ signals. Invertebrate deuterostome eggs display mainly three distinct types of cell cycle arrest before fertilization mediated by potentially different cytostatic factors (CSF): one CSF causes arrest during meiotic metaphase I (MI-CSF in tunicates and some starfishes), another CSF likely causes arrest during meiotic metaphase II (amphioxus), and yet another form of CSF causes arrest to occur after meiotic exit during G1 of the first mitotic cycle (G1-CSF). In tunicates and echinoderms these different CSF activities have been shown to rely on the Mos//MAPK pathway for establishment and on Ca2+ signals for their inactivation. Despite these molecular similarities, release of MI-CSF arrest is caused by APC/C activation (to destroy cyclin B) whereas release from G1-CSF is caused by stimulating S phase and the synthesis of cyclins. Further research is needed to understand how both the Mos//MAPK cascade and Ca2+ achieve these tasks in different marine invertebrate deuterostomes. Another conserved feature of eggs is that protein synthesis of specific mRNAs is necessary to proceed through oocyte maturation and to maintain CSF-induced cell cycle arrest. Then activation of development at fertilization is accompanied by an increase in the rate of protein synthesis but the mechanisms involved are still largely unknown in most of the marine deuterostomes. How the sperm-triggered Ca2+ signals cause an increase in protein synthesis has been studied mainly in sea urchin eggs. Here we review these conserved features of eggs (arrest, activation and protein synthesis) focusing on the non-vertebrate deuterostomes. PMID:24721426

  14. Cell cycle arrest and activation of development in marine invertebrate deuterostomes.

    PubMed

    Costache, Vlad; McDougall, Alex; Dumollard, Rémi

    2014-08-01

    Like most metazoans, eggs of echinoderms and tunicates (marine deuterostomes, there is no data for the cephalochordates) arrest awaiting fertilization due to the activity of the Mos/MEK/MAPK cascade and are released from this cell cycle arrest by sperm-triggered Ca2+ signals. Invertebrate deuterostome eggs display mainly three distinct types of cell cycle arrest before fertilization mediated by potentially different cytostatic factors (CSF): one CSF causes arrest during meiotic metaphase I (MI-CSF in tunicates and some starfishes), another CSF likely causes arrest during meiotic metaphase II (amphioxus), and yet another form of CSF causes arrest to occur after meiotic exit during G1 of the first mitotic cycle (G1-CSF). In tunicates and echinoderms these different CSF activities have been shown to rely on the Mos//MAPK pathway for establishment and on Ca2+ signals for their inactivation. Despite these molecular similarities, release of MI-CSF arrest is caused by APC/C activation (to destroy cyclin B) whereas release from G1-CSF is caused by stimulating S phase and the synthesis of cyclins. Further research is needed to understand how both the Mos//MAPK cascade and Ca2+ achieve these tasks in different marine invertebrate deuterostomes. Another conserved feature of eggs is that protein synthesis of specific mRNAs is necessary to proceed through oocyte maturation and to maintain CSF-induced cell cycle arrest. Then activation of development at fertilization is accompanied by an increase in the rate of protein synthesis but the mechanisms involved are still largely unknown in most of the marine deuterostomes. How the sperm-triggered Ca2+ signals cause an increase in protein synthesis has been studied mainly in sea urchin eggs. Here we review these conserved features of eggs (arrest, activation and protein synthesis) focusing on the non-vertebrate deuterostomes.

  15. Studies in Low-Energy Nuclear Science

    SciTech Connect

    Brune, Carl R; Grimes, Steven M

    2006-03-30

    This report presents a summary of research projects in the area of low energy nuclear reactions and structure, carried out between 1 January 2003 and 31 December 2005 and supported by U.S. DOE grant number DE-FG03-03NA00074. Cross sections measured with high resolution have been subjected to an Ericson theory analysis to infer information about the nuclear level density. Other measurements were made of the spectral shape of particles produced in evaporation processes; these also yield level density information. A major project was the development of a new Hauser-Feshbach code for analyzing such spectra. Other measurements produced information on the spectra of gamma rays emitted in reactions on heavy nuclei and gave a means of refining our understanding of gamma-ray strength functions. Finally,reactions on light nuclei were studied and subjected to an R-matrix analysis. Cross sections fora network of nuclear reactions proceedingthrough a given compound nucleus shouldgreatly constrain the family of allowed parameters. Modifications to the formalism andcomputer code are also discussed.

  16. Low energy scattering with a nontrivial pion

    SciTech Connect

    Fariborz, Amir H.

    2007-12-01

    An earlier calculation in a generalized linear sigma model showed that the well-known current algebra formula for low energy pion-pion scattering held even though the massless Nambu Goldstone pion contained a small admixture of a two-quark two-antiquark field. Here we turn on the pion mass and note that the current algebra formula no longer holds exactly. We discuss this small deviation and also study the effects of a SU(3) symmetric quark mass type term on the masses and mixings of the eight SU(3) multiplets in the model. We calculate the s-wave scattering lengths, including the beyond current algebra theorem corrections due to the scalar mesons, and observe that the effect of the scalar mesons is to improve the agreement with experiment. In the process, we uncover the way in which linear sigma models give controlled corrections (due to the presence of scalar mesons) to the current algebra scattering formula. Such a feature is commonly thought to exist only in the nonlinear sigma model approach.

  17. Low energy stable plasma calibration facility.

    PubMed

    Frederick-Frost, K M; Lynch, K A

    2007-07-01

    We have designed and fabricated a low energy plasma calibration facility for testing and calibration of rocket-borne charged-particle detectors and for the investigation of plasma sheath formation in an environment with ionospheric plasma energies, densities, and Debye lengths. We describe the vacuum system and associated plasma source, which was modified from a Naval Research Laboratory design [Bowles et al. Rev. Sci. Instrum. 67, 455 (1996)]. Mechanical and electrical modifications to this cylindrical microwave resonant source are outlined together with a different method of operating the magnetron that achieves a stable discharge. This facility produces unmagnetized plasmas with densities from 1x10(3)/cm(3) to 6x10(5)/cm(3), electron temperatures from 0.1 to 1.7 eV, and plasma potentials from 0.5 to 8 V depending on varying input microwave power and neutral gas flow. For the range of input microwave power explored (350-600 W), the energy density of the plasma remains constant because of an inverse relationship between density and temperature. This relationship allows a wide range of Debye lengths (0.3-8.4 cm) to be investigated, which is ideal for simulating the ionospheric plasma sheaths we explore.

  18. Low energy CMOS for space applications

    NASA Technical Reports Server (NTRS)

    Panwar, Ramesh; Alkalaj, Leon

    1992-01-01

    The current focus of NASA's space flight programs reflects a new thrust towards smaller, less costly, and more frequent space missions, when compared to missions such as Galileo, Magellan, or Cassini. Recently, the concept of a microspacecraft was proposed. In this concept, a small, compact spacecraft that weighs tens of kilograms performs focused scientific objectives such as imaging. Similarly, a Mars Lander micro-rover project is under study that will allow miniature robots weighing less than seven kilograms to explore the Martian surface. To bring the microspacecraft and microrover ideas to fruition, one will have to leverage compact 3D multi-chip module-based multiprocessors (MCM) technologies. Low energy CMOS will become increasingly important because of the thermodynamic considerations in cooling compact 3D MCM implementations and also from considerations of the power budget for space applications. In this paper, we show how the operating voltage is related to the threshold voltage of the CMOS transistors for accomplishing a task in VLSI with minimal energy. We also derive expressions for the noise margins at the optimal operating point. We then look at a low voltage CMOS (LVCMOS) technology developed at Stanford University which improves the power consumption over conventional CMOS by a couple of orders of magnitude and consider the suitability of the technology for space applications by characterizing its SEU immunity.

  19. Oscillations of very low energy atmospheric neutrinos

    SciTech Connect

    Peres, Orlando L. G.; Smirnov, A. Yu.

    2009-06-01

    There are several new features in the production, oscillations, and detection of the atmospheric neutrinos of low energies E < or approx. 100 MeV. The flavor ratio r of muon to electron neutrino fluxes is substantially smaller than 2 and decreases with energy, a significant part of events is due to the decay of invisible muons at rest, etc. Oscillations in a two-layer medium (atmosphere-Earth) should be taken into account. We derive analytical and semianalytical expressions for the oscillation probabilities of these 'sub-sub-GeV' neutrinos. The energy spectra of the e-like events in water Cherenkov detectors are computed, and the dependence of the spectra on the 2-3 mixing angle {theta}{sub 23}, the 1-3 mixing, and the CP-violation phase are studied. We find that variations of {theta}{sub 23} in the presently allowed region change the number of e-like events by about 15%-20% as well as lead to distortion of the energy spectrum. The 1-3 mixing and CP violation can lead to {approx}10% effects. Detailed study of the sub-sub-GeV neutrinos will be possible in future megaton-scale detectors.

  20. Low energy beam transport system developments

    NASA Astrophysics Data System (ADS)

    Dudnikov, V.; Han, B.; Stockli, M.; Welton, R.; Dudnikova, G.

    2015-04-01

    For high brightness beam production it is important to preserve the brightness in the low energy beam transport system (LEBT) used to transport and match the ion beams to the next stage of acceleration, usually an RFQ. While electrostatic focusing can be problematic for high current beam transport, reliable electrostatic LEBT operation has been demonstrated with H- beams up to 60 mA. Now, however, it is commonly accepted that an optimal LEBT for high current accelerator applications consists of focusing solenoids with space charge compensation. Two-solenoid LEBTs are successfully used for high current (>100 mA) proton beam transport. Preservation of low emittances (~0.15 π mm-mrad) requires the addition of a heavy gas (Xe, Kr), which causes ~5% of proton loss in a 1 m long LEBT. Similar Xe densities would be required to preserve low emittances of H- beams, but such gas densities cause unacceptably high H- beam losses. A short LEBT with only one short solenoid, movable for RFQ matching, can be used for reduced negative ion stripping. A strong electrostatic-focusing LEBT has been successfully adopted for transport of high current H- beams in the SNS Front End. Some modifications of such electrostatic LEBTs are expected to improve the reliable transport of intense positive and negative ion beams without greatly degrading their low emittances. We concentrate on processes that determine the beam brightness degradation and on their prevention. Proposed improvements to the SNS electrostatic LEBT are discussed.

  1. Low Energy Electron Scattering from Fuels

    NASA Astrophysics Data System (ADS)

    Lopes, M. Cristina A.

    2012-06-01

    We report an investigation of processes that occur during the ignition of the plasma and its consequences in post-discharge time for an internal combustion engine, in order to find the appropriate parameters to be used in cars that operate with lean mixtures air-fuel. The relevance of this theme has attracted much attention, and has been one of the subjects of collaboration between experimental and theoretical groups in the USA and Brazil. We have produced some basic information necessary to modeling spark ignition in alcohol- fuelled engines. Total cross sections of electron scattering by methanol and ethanol molecules were obtained, using the linear transmission method based on the Beer-Lambert law to first approximation. Measurements and calculations of differential cross sections for low-energy (rotationally unresolved) electron scattering were also obtained, for scattering angles of 5 --130 . The measurements were taken using the relative flow method with an aperture source, and calculations using two different implementations of the Schwinger multichannel method, one that takes all electrons into account and is adapted for parallel computers, and another that uses pseudopotentials and considers only the valence electrons. Additionally to these, computer simulation studies of electronic discharge in mixtures of ethanol were performed, using a Zero-Dimensional Plasma Kinetic solver. Previous reported models for combustion of ethanol and cross sections data for momentum transfer of electron collisions with ethanol were used. The time evolutions of the main species densities are reported and the ignition time delay discussed.

  2. Low-energy electron-atom bremsstrahlung

    NASA Technical Reports Server (NTRS)

    Gould, R. J.

    1986-01-01

    This paper extends recent work providing an elementary calculation of bremsstrahlung and opacity associated with the scattering of low-energy electrons by neutral atoms and molecules. The method applies when the scattering potential is 'hard' or when the collision time is short, applying the classical soft-photon emission probability formula for arbitrary bremsstrahlung photon energy. However, now, in addition to correcting the probability factor for finite bremsstrahlung photon energy, another factor corrects for the reduced phase space available to the outgoing electron. The bremsstrahlung cross section and opacity are then computed directly from the elastic scattering cross section, determined experimentally or calculated; a small (approximately 10 percent) correction is computed from the anisotropic term in the elastic scattering cross section. The opacity is evaluated for electron scattering by H, He, and H2 using experimentally determined values for the elastic scattering cross section, and is compared with more elaborate calculations. The agreement is good (within 10 percent), indicating an accuracy for the general method comparable to variations among the results of different elaborate theoretical computations. The agreement seems to validate the basic approximation of short collision time even at large bremsstrahlung photon energy for electron energies and temperatures up to a few eV.

  3. Optimal Low Energy Earth-Moon Transfers

    NASA Technical Reports Server (NTRS)

    Griesemer, Paul Ricord; Ocampo, Cesar; Cooley, D. S.

    2010-01-01

    The optimality of a low-energy Earth-Moon transfer is examined for the first time using primer vector theory. An optimal control problem is formed with the following free variables: the location, time, and magnitude of the transfer insertion burn, and the transfer time. A constraint is placed on the initial state of the spacecraft to bind it to a given initial orbit around a first body, and on the final state of the spacecraft to limit its Keplerian energy with respect to a second body. Optimal transfers in the system are shown to meet certain conditions placed on the primer vector and its time derivative. A two point boundary value problem containing these necessary conditions is created for use in targeting optimal transfers. The two point boundary value problem is then applied to the ballistic lunar capture problem, and an optimal trajectory is shown. Additionally, the ballistic lunar capture trajectory is examined to determine whether one or more additional impulses may improve on the cost of the transfer.

  4. RHIC low-energy challenges and plans

    SciTech Connect

    Satogata,T.; Ahrens, L.; Bai, M.; Brennan, J.M.; Bruno, D.; Butler, J.; Drees, A.; Fedotov, A.; Fischer, W.; Harvey, M.; Hayes, T.; Jappe, W.; Lee, R.C.; MacKay, W.W.; Malitsky, N.; Marr, G.; Michnoff, R.; Oerter, B.; Pozdeyev, E.; Roser, T.; Schoefer, V.; Severino, F.; Smith, K.; Tepikian, S.; Tsoupas, N.

    2009-06-08

    Future Relativistic Heavy Ion Collider (RHIC) runs, including a portion of FY10 heavy ion operations, will explore collisions at center of mass energies of 5-50 GeV/n (GeV/nucleon). Operations at these energies is motivated by the search for a possible QCD phase transition critical point. The lowest end of this energy range is nearly a factor of four below the nominal RHIC injection center of mass energy {radical}s = 19.6 GeV/n. There are several operational challenges in the RHIC low-energy regime, including harmonic number changes, small longitudinal acceptance, lowered magnet field quality, nonlinear orbit control, and luminosity monitoring. We report on the experience with these challenges during beam tests with gold beams in March 2008. This includes first operations at {radical}s = 9.18 GeV/n, first beam experience at {radical}s = 5 GeV/n, and luminosity projections for near-term operations.

  5. Low Energy Transfer to the Moon

    NASA Astrophysics Data System (ADS)

    Koon, W. S.; Lo, M. W.; Marsden, J. E.; Ross, S. D.

    2001-11-01

    New space missions are increasingly more complex; demand for exotic orbits to solve engineering problems has grown beyond the existing astrodynamic infrastructure based on two-body interactions. The delicate heteroclinic dynamics used by the Genesis Mission dramatically illustrate the need for a new paradigm: dynamical system study of three-body problem. Furthermore, this dynamics has much to say about the morphology and transport of materials within the Solar System. The cross-fertilization of ideas between the natural dynamics of the Solar System and applications to engineering has produced new techniques for constructing spacecraft trajectories with interesting characteristics. Specifically, these techniques are used here to produce a lunar capture mission which uses less fuel than a Hohmann transfer. We approximate the Sun-Earth-Moon-Spacecraft four-body problem as two three-body problems. Using the invariant manifold structures of the Lagrange points of the three-body systems, we are able to construct low energy transfer trajectories from the Earth which exhibit ballistic capture at the Moon. The techniques used in the design and construction of this trajectory may be applied in many situations. This is joint work with Martin W. Lo, Jerrold E. Marsden and Shane D. Ross and was partially supported by the National Science Foundation Grant No. KFI/ATM-9873133 under a contract with the Jet Propulsion Laboratory, NASA.

  6. Low Energy Electron Scattering from Fuels

    NASA Astrophysics Data System (ADS)

    Lopes, M. C. A.; Silva, D. G. M.; Bettega, M. H. F.; da Costa, R. F.; Lima, M. A. P.; Khakoo, M. A.; Winstead, C.; McKoy, V.

    2012-11-01

    In order to understand and optimize processes occurring during the ignition of plasma and its consequences in post-discharge for an internal combustion engine, especially considering the spark plug, we have produced in this work some basic information necessary to modeling spark ignition in alcohol- fuelled engines. Total cross sections of electron scattering by methanol and ethanol molecules in the energy range from 60 to 500 eV are reported, using the linear transmission method based on the Beer-Lambert law to first approximation. Aditionally to that, measurements and calculations of differential cross sections for elastic low-energy (rotationally unresolved) electron scattering were also discussed, for impact energies of 1, 2, 5, 10, 15, 20, 30, 50, and 100 eV and for scattering angles of 5°-130°. The measurements were obtained using the relative flow method with an aperture source, and calculations using two different implementations of the Schwinger multichannel method, one that takes all electrons into account and is adapted for parallel computers, and another that uses pseudopotentials and considers only the valence electrons.

  7. Low-energy control of electrical turbulence in the heart

    NASA Astrophysics Data System (ADS)

    Luther, Stefan; Fenton, Flavio H.; Kornreich, Bruce G.; Squires, Amgad; Bittihn, Philip; Hornung, Daniel; Zabel, Markus; Flanders, James; Gladuli, Andrea; Campoy, Luis; Cherry, Elizabeth M.; Luther, Gisa; Hasenfuss, Gerd; Krinsky, Valentin I.; Pumir, Alain; Gilmour, Robert F.; Bodenschatz, Eberhard

    2011-07-01

    Controlling the complex spatio-temporal dynamics underlying life-threatening cardiac arrhythmias such as fibrillation is extremely difficult, because of the nonlinear interaction of excitation waves in a heterogeneous anatomical substrate. In the absence of a better strategy, strong, globally resetting electrical shocks remain the only reliable treatment for cardiac fibrillation. Here we establish the relationship between the response of the tissue to an electric field and the spatial distribution of heterogeneities in the scale-free coronary vascular structure. We show that in response to a pulsed electric field, E, these heterogeneities serve as nucleation sites for the generation of intramural electrical waves with a source density ρ(E) and a characteristic time, τ, for tissue depolarization that obeys the power law τ~Eα. These intramural wave sources permit targeting of electrical turbulence near the cores of the vortices of electrical activity that drive complex fibrillatory dynamics. We show in vitro that simultaneous and direct access to multiple vortex cores results in rapid synchronization of cardiac tissue and therefore, efficient termination of fibrillation. Using this control strategy, we demonstrate low-energy termination of fibrillation in vivo. Our results give new insights into the mechanisms and dynamics underlying the control of spatio-temporal chaos in heterogeneous excitable media and provide new research perspectives towards alternative, life-saving low-energy defibrillation techniques.

  8. Surface Coordination of Adatoms by Scanned Low Energy Photoelectron Diffraction

    NASA Astrophysics Data System (ADS)

    Asensio, M. C.

    In this article, a brief overview of the current activity in the field of low energy photoelectron diffraction is presented. Although alternatively angle and energy-scanned photoelectron diffraction can be used to obtain the surface-structural information, we limit our discussion to the low energy and energy-scanned modes and their use in connection with a new developed direct method. By the use of this most recent approach, adatom-substrate distances and adsorption sites are directly revealed from a discrete mapping of the Fourier transform of scanned energy photoelectron diffraction spectra, measured at a representative set of geometries, which depend on the symmetry of the particular studied system. In addition, a short discussion on the determination of the detailed structure of adsorbed overlayers by the traditional trial-and-error method is included, using model multiple scattering calculations. These latest developments are illustrated with a specific example of an atomic adsorbate, and comments about the capabilities and limitations of photoelectron diffraction as a structural technique in new fields.

  9. New Cooperative Mechanisms of Low-Energy Nuclear Reactions Using Super Low-Energy External Field

    NASA Astrophysics Data System (ADS)

    Gareev, F. A.; Zhidkova, I. E.

    We propose a new mechanism of LENR: cooperative processes in the whole system, nuclei + atoms + condensed matter, can occur at a smaller threshold energies than the corresponding ones on free constituents. The cooperative processes can be induced and enhanced by low-energy external fields. The excess heat is the emission of internal energy and transmutations at LENR are the result of redistribution of inner energy of the whole system.

  10. Quantifying Low Energy Proton Damage in Multijunction Solar Cells

    NASA Technical Reports Server (NTRS)

    Messenger, Scott R.; Burke, Edward A.; Walters, Robert J.; Warner, Jeffrey H.; Summers, Geoffrey P.; Lorentzen, Justin R.; Morton, Thomas L.; Taylor, Steven J.

    2007-01-01

    An analysis of the effects of low energy proton irradiation on the electrical performance of triple junction (3J) InGaP2/GaAs/Ge solar cells is presented. The Monte Carlo ion transport code (SRIM) is used to simulate the damage profile induced in a 3J solar cell under the conditions of typical ground testing and that of the space environment. The results are used to present a quantitative analysis of the defect, and hence damage, distribution induced in the cell active region by the different radiation conditions. The modelling results show that, in the space environment, the solar cell will experience a uniform damage distribution through the active region of the cell. Through an application of the displacement damage dose analysis methodology, the implications of this result on mission performance predictions are investigated.

  11. 75 FR 32398 - Taking of Marine Mammals Incidental to Specified Activities; U.S. Marine Corps Training Exercises...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ... Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), Commerce. ACTION... arms, large arms, bombs, rockets, missiles, and pyrotechnics. All munitions used at BT-11 are inert... designated areas of rocket firing, target practice, or other hazardous operations (33 CFR 334.420)....

  12. 76 FR 71535 - Taking of Marine Mammals Incidental to Specified Activities; U.S. Marine Corps Training Exercises...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ... Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), Commerce. ACTION..., beginning December 1, 2011 (75 FR 72807; November 26, 2010). Weapon delivery training would occur at two BTs..., rockets, missiles, and pyrotechnics. All munitions used at BT-11 are inert, practice rounds. No...

  13. 78 FR 71566 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-29

    ... authorization (78 FR 52148; August 22, 2013; hereafter, the FR notice); please see that document or the Navy's... provided in the FR notice (78 FR 52148; August 22, 2013). Significant sound- producing in-water... in disturbance to marine mammals in the project area. Please see the FR notice (78 FR 52148;...

  14. 78 FR 30273 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... potential area of effect for this project (London, 2006; see Figure 4-1 of the Navy's application.... waters and just south of the U.S.-Mexico border, and animals belonging to this population may be found... application from the U.S. Navy (Navy) for an Incidental Harassment Authorization (IHA) to take marine...

  15. 78 FR 29705 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-21

    ... application from the U.S. Navy (Navy) for an Incidental Harassment Authorization (IHA) to take marine mammals....noaa.gov/pr/permits/incidental.htm . Supplemental documents provided by the U.S. Navy may be found at..., (301) 427-8401. SUPPLEMENTARY INFORMATION: Background Sections 101(a)(5)(A) and (D) of the MMPA (16...

  16. 78 FR 56659 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-13

    ... U.S. waters and just south of the U.S.-Mexico border, and animals belonging to this population may... from the U.S. Navy (Navy) for authorization to take marine mammals incidental to construction..., NMFS, (301) 427-8401. SUPPLEMENTARY INFORMATION: Availability A copy of the Navy's application and...

  17. Update Heat injuries, active component, U.S. Army, Navy, Air Force, and Marine Corps, 2015.

    PubMed

    2016-03-01

    The incidence rate of heat stroke among active component members of the U.S. Army, Navy, Air Force, and Marine Corps in 2015 was higher than rates in the previous 4 years. Incidence rates of heat stroke were higher among males, those younger than 20 years of age, Asian/Pacific Islanders, Marine Corps and Army members, and service members in combat-specific occupations, compared to their respective counterparts. More service members were treated for "other heat injuries" in 2015 (n=1,933) than in either of the previous 2 years. The incidence rate of "other heat injuries" was higher among females than males and rates were highest among service members younger than 20 years of age, among Army and Marine Corps members, among recruit trainees, and among service members in combat-specific occupations. During 2011-2015, 720 diagnoses of heat injuries were documented among service members serving in Iraq/Afghanistan; 6.9% (n=50) of those diagnoses were for heat stroke. PMID:27030928

  18. Antimicrobial activities of novel cultivable bacteria isolated from marine sponge Tedania anhelans

    NASA Astrophysics Data System (ADS)

    Zeng, Zhen; Zhao, Jing; Ke, Caihuan; Wang, Dexiang

    2013-05-01

    Marine sponge Tedania anhelans distributes throughout the intertidal zone of Fujian, southeastern China, and is a potential source of natural bioactive products. The sponge harbors a large number of bacterial groups that have been identified using various techniques, including fluorescent in situ hybridization (FISH). Fractionation of dissociated sponge allowed isolation of 25 bacterial species. Based on 16S rRNA gene sequencing, phylogenetic analysis attributed most of these eubacteria to α- Proteobacteria, γ- Proteobacteria, Cytophaga / Flavobacterium / Bacteroidetes (CFB group), and the family Bacillaceae of Gram-positive bacteria. In sequence similarity, five putatively novel species were identified with less than 98% similarity to other strains in the NCBI database. Tests for antimicrobial activities were performed against Gram-positive bacteria, Gram-negative bacteria, fungi, antitumor indicators Escherichia coli 343/591 (with DNA repair deficiency), regular E. coli 343/636 (with different DNA repair capacity), and 10 bacterial isolates exhibited inhibitory bioactivities. Among these strains, three isolates were detected involving function gene NRPS-A domains, which were most closely related to the amino acid sequences of linear gramicidin synthetase and pyoverdine synthetase. These results contribute to our knowledge of the microbes associated with marine sponges and further reveal novel bacterial resources for the screening of bioactive marine natural products.

  19. Synthesis and structure-activity relationship of cytotoxic marine cyclodepsipeptide IB-01212 analogues.

    PubMed

    Cruz, Luis J; Francesch, Andres; Cuevas, Carmen; Albericio, Fernando

    2007-07-01

    Several recently discovered marine products have remarkable in vitro and in vivo anticancer profiles against a wide range of tumor cell lines. Some of these compounds are currently in clinical trials. These compounds show complex structures and mechanisms of action of interest. Herein, we describe the preparation of a series of totally synthetic molecules that are structurally related to the natural marine product IB-01212 and evaluated them as antitumor agents. For this, total solid-phase syntheses of the products were performed in parallel by two distinct routes: linear synthesis and convergent synthesis. Structural modifications were introduced in several residue positions to afford 21 IB-01212 analogues for structure-relationship studies. An increase in the number of methyl groups in the macrocycle enhanced cytotoxic activity. Also, the replacement of an ester bond by an amide bond favored antitumor activity against several human cell lines. In addition, the L configuration analogues were more active against all the tumor cell lines than those containing the D configuration. A significant increase in the size and asymmetry of the macrocycle diminished biological activity with respect to that of IB-01212. These results are of great value for the discovery of new and more effective anticancer agents.

  20. Antibacterial Activity of Marine and Black Band Disease Cyanobacteria against Coral-Associated Bacteria

    PubMed Central

    Gantar, Miroslav; Kaczmarsky, Longin T.; Stanić, Dina; Miller, Aaron W.; Richardson, Laurie L.

    2011-01-01

    Black band disease (BBD) of corals is a cyanobacteria-dominated polymicrobial disease that contains diverse populations of heterotrophic bacteria. It is one of the most destructive of coral diseases and is found globally on tropical and sub-tropical reefs. We assessed ten strains of BBD cyanobacteria, and ten strains of cyanobacteria isolated from other marine sources, for their antibacterial effect on growth of heterotrophic bacteria isolated from BBD, from the surface mucopolysaccharide layer (SML) of healthy corals, and three known bacterial coral pathogens. Assays were conducted using two methods: co-cultivation of cyanobacterial and bacterial isolates, and exposure of test bacteria to (hydrophilic and lipophilic) cyanobacterial cell extracts. During co-cultivation, 15 of the 20 cyanobacterial strains tested had antibacterial activity against at least one of the test bacterial strains. Inhibition was significantly higher for BBD cyanobacteria when compared to other marine cyanobacteria. Lipophilic extracts were more active than co-cultivation (extracts of 18 of the 20 strains were active) while hydrophilic extracts had very limited activity. In some cases co-cultivation resulted in stimulation of BBD and SML bacterial growth. Our results suggest that BBD cyanobacteria are involved in structuring the complex polymicrobial BBD microbial community by production of antimicrobial compounds. PMID:22073011

  1. Molluscicidal activity of some marine substances against the snail Biomphalaria glabrata (Mollusca, Planorbidae).

    PubMed

    Miyasato, P A; Kawano, T; Freitas, J C; Berlinck, R G S; Nakano, E; Tallarico, L F

    2012-05-01

    Freshwater snails of the genus Biomphalaria play a major role as intermediate hosts of Schistosoma mansoni, the etiologic agent of schistosomiasis. While Biomphalaria spp. control by molluscicides is one of the main strategies to reduce the snail population in infected areas, there are few effective molluscicides commercially available. Natural products may be considered as potentially useful and safe molluscicides. We have evaluated the molluscicidal activity of 12 extracts from ten marine organisms on adult and embryonic stages of Biomphalaria glabrata. Only extracts of the red algae Liagora farinosa and of the sponge Amphimedon viridis presented molluscicidal activity. Lethal concentration (LC)(50) values obtained were 120 μg/mL for L. farinosa CH(2)Cl(2) extract (apolar fraction) and 20 μg/mL for A. viridis extract and halitoxin. The polar alga fraction and halitoxin had no effect on B. glabrata embryos. The algae apolar fraction was active on B. glabrata in all embryonic development stages, with LC(50) values for blastulae at 42 μg/mL, gastrulae at 124 μg/mL, trochophore at 180 μg/mL, and veliger at 222 μg/mL. This is the first report of extracts from marine organisms which presented molluscicidal activity. PMID:22205347

  2. Recent Articles, Activities, and Other Documents in the Marine Education Field.

    ERIC Educational Resources Information Center

    Schlenker, Richard M.

    This annotated bibliography of marine education contains 35 documents published in 1976-1977 and an unpublished masters thesis (1969). The majority of the works are laboratory projects in marine science for students at the elementary and secondary school level. Other entries include marine science units for science teachers, marine education grant…

  3. Active Marine Subsurface Bacterial Population Composition in Low Organic Carbon Environments from IODP Expedition 320

    NASA Astrophysics Data System (ADS)

    Shepard, A.; Reese, B. K.; Mills, H. J.; IODP Expedition 320 Shipboard Science Party

    2011-12-01

    The marine subsurface environment contains abundant and active microorganisms. These microbial populations are considered integral players in the marine subsurface biogeochemical system with significance in global geochemical cycles and reservoirs. However, variations in microbial community structure, activity and function associated with the wide-ranging sedimentary and geochemical environments found globally have not been fully resolved. Integrated Ocean Drilling Program Expedition 320 recovered sediments from site U1332. Two sampling depths were selected for analysis that spanned differing lithological units in the sediment core. Sediments were composed of mostly clay with zeolite minerals at 8 meters below sea floor (mbsf). At 27 mbsf, sediments were composed of alternating clayey radiolarian ooze and nannofossil ooze. The concentration of SO42- had little variability throughout the core and the concentration of Fe2+ remained close to, or below, detection limits (0.4 μM). Total organic carbon content ranged from a low of 0.03 wt% to a high of 0.07 wt% between 6 and 30 mbsf providing an opportunity to evaluate marine subsurface microbial communities under extreme electron donor limiting conditions. The metabolically active fraction of the bacterial population was isolated by the extraction and amplification of 16S ribosomal RNA. Pyrosequencing of 16S rRNA transcripts and subsequent bioinformatic analyses provided a robust data set (15,931 total classified sequences) to characterize the community at a high resolution. As observed in other subsurface environments, the overall diversity of active bacterial populations decreased with depth. The population shifted from a diverse but evenly distributed community at approximately 8 mbsf to a Firmicutes dominated population at 27 mbsf (80% of sequences). A total of 95% of the sequences at 27 mbsf were grouped into three genera: Lactobacillus (phylum Firmicutes) at 80% of the total sequences, Marinobacter (phylum

  4. Low energy beam transport system developments

    SciTech Connect

    Dudnikov, V.; Han, B.; Stockli, M.; Welton, R.; Dudnikova, G.

    2015-04-08

    For high brightness beam production it is important to preserve the brightness in the low energy beam transport system (LEBT) used to transport and match the ion beams to the next stage of acceleration, usually an RFQ. While electrostatic focusing can be problematic for high current beam transport, reliable electrostatic LEBT operation has been demonstrated with H{sup −} beams up to 60 mA. Now, however, it is commonly accepted that an optimal LEBT for high current accelerator applications consists of focusing solenoids with space charge compensation. Two-solenoid LEBTs are successfully used for high current (>100 mA) proton beam transport. Preservation of low emittances (~0.15 π mm-mrad) requires the addition of a heavy gas (Xe, Kr), which causes ~5% of proton loss in a 1 m long LEBT. Similar Xe densities would be required to preserve low emittances of H{sup −} beams, but such gas densities cause unacceptably high H{sup −} beam losses. A short LEBT with only one short solenoid, movable for RFQ matching, can be used for reduced negative ion stripping. A strong electrostatic-focusing LEBT has been successfully adopted for transport of high current H{sup −} beams in the SNS Front End. Some modifications of such electrostatic LEBTs are expected to improve the reliable transport of intense positive and negative ion beams without greatly degrading their low emittances. We concentrate on processes that determine the beam brightness degradation and on their prevention. Proposed improvements to the SNS electrostatic LEBT are discussed.

  5. Ectoenzyme activity in coastal marine waters: response to temperature and metal ion availability

    NASA Astrophysics Data System (ADS)

    Wiedenbeck, J. K.; Neino, V.; Allison, S. D.; Martiny, A.

    2009-12-01

    Ectoenzymes in the ocean are vital for the breakdown of complex organic substrates and for the uptake of nutrients by marine organisms. The activity levels of these enzymes affect the turnover rate of nutrient pools within the ocean, and thus have a significant impact on global biogeochemical nutrient cycles. This study measured the activity of extracellular enzymes from seawater samples under different environmental conditions. Samples were collected daily from coastal waters in the subtropical North Pacific (Lat.: 33°). Ambient seawater temperatures were between 18° and 20° C for the duration of the study. The activity response of four enzymes (alkaline phosphatase, β-glucosidase, β-N-acetyl glucosaminidase, and leucine aminopeptidase) was measured over a range of temperatures (4° to 40° C). The optimal temperatures of all four enzymes were above the ambient seawater temperature of the samples: optimal temperatures of β-glucosidase, β-N-acetyl glucosaminidase, and leucine aminopeptidase in the seawater samples were between 28° and 34° C, while alkaline phosphatase activity increased with the temperature over the range tested. Enzymatic activity of alkaline phosphatase was further investigated under several metal ion conditions. Activity was highest in the presence of Co2+ ions, while the availability of other ions (Ca2+ and Mg2+/Zn2+) had a lesser effect. The influence of Co2+ on alkaline phosphatase activity indicates the presence of a Co2+-dependent alkaline phosphatase in coastal marine waters. These results suggest that variations in environmental conditions (such as temperature and ion concentration) have discernable effects on enzyme activity, and thus affect turnover rates of nutrient pools in the ocean.

  6. Tracking small mountainous river derived terrestrial organic carbon across the active margin marine environment

    NASA Astrophysics Data System (ADS)

    Childress, L. B.; Blair, N. E.; Orpin, A. R.

    2015-12-01

    Active margins are particularly efficient in the burial of organic carbon due to the close proximity of highland sources to marine sediment sinks and high sediment transport rates. Compared with passive margins, active margins are dominated by small mountainous river systems, and play a unique role in marine and global carbon cycles. Small mountainous rivers drain only approximately 20% of land, but deliver approximately 40% of the fluvial sediment to the global ocean. Unlike large passive margin systems where riverine organic carbon is efficiently incinerated on continental shelves, small mountainous river dominated systems are highly effective in the burial and preservation of organic carbon due to the rapid and episodic delivery of organic carbon sourced from vegetation, soil, and rock. To investigate the erosion, transport, and burial of organic carbon in active margin small mountainous river systems we use the Waipaoa River, New Zealand. The Waipaoa River, and adjacent marine depositional environment, is a system of interest due to a large sediment yield (6800 tons km-2 yr-1) and extensive characterization. Previous studies have considered the biogeochemistry of the watershed and tracked the transport of terrestrially derived sediment and organics to the continental shelf and slope by biogeochemical proxies including stable carbon isotopes, lignin phenols, n-alkanes, and n-fatty acids. In this work we expand the spatial extent of investigation to include deep sea sediments of the Hikurangi Trough. Located in approximately 3000 m water depth 120 km from the mouth of the Waipaoa River, the Hikurangi Trough is the southern extension of the Tonga-Kermadec-Hikurangi subduction system. Piston core sediments collected by the National Institute of Water and Atmospheric Research (NIWA, NZ) in the Hikurangi Trough indicate the presence of terrestrially derived material (lignin phenols), and suggest a continuum of deposition, resuspension, and transport across the margin

  7. Macrolactone Nuiapolide, Isolated from a Hawaiian Marine Cyanobacterium, Exhibits Anti-Chemotactic Activity

    PubMed Central

    Mori, Shogo; Williams, Howard; Cagle, Davey; Karanovich, Kristopher; Horgen, F. David; Smith, Roger; Watanabe, Coran M. H.

    2015-01-01

    A new bioactive macrolactone, nuiapolide (1) was identified from a marine cyanobacterium collected off the coast of Niihau, near Lehua Rock. The natural product exhibits anti-chemotactic activity at concentrations as low as 1.3 μM against Jurkat cells, cancerous T lymphocytes, and induces a G2/M phase cell cycle shift. Structural characterization of the natural product revealed the compound to be a 40-membered macrolactone with nine hydroxyl functional groups and a rare tert-butyl carbinol residue. PMID:26473885

  8. Synthesis and Anti-Tuberculosis Activity of the Marine Natural Product Caulerpin and Its Analogues

    PubMed Central

    Canché Chay, Cristina I.; Gómez Cansino, Rocío; Espitia Pinzón, Clara I.; Torres-Ochoa, Rubén O.; Martínez, Roberto

    2014-01-01

    Caulerpin (1a), a bis-indole alkaloid from the marine algal Caulerpa sp., was synthesized in three reaction steps with an overall yield of 11%. The caulerpin analogues (1b–1g) were prepared using the same synthetic pathway with overall yields between 3% and 8%. The key reaction involved a radical oxidative aromatic substitution involving xanthate (3) and 3-formylindole compounds (4a–4g). All bis-indole compounds synthesized were evaluated against the Mycobacterium tuberculosis strain H37Rv, and 1a was found to display excellent activity (IC50 0.24 µM). PMID:24681629

  9. Macrolactone Nuiapolide, Isolated from a Hawaiian Marine Cyanobacterium, Exhibits Anti-Chemotactic Activity.

    PubMed

    Mori, Shogo; Williams, Howard; Cagle, Davey; Karanovich, Kristopher; Horgen, F David; Iii, Roger Smith; Watanabe, Coran M H

    2015-10-01

    A new bioactive macrolactone, nuiapolide (1) was identified from a marine cyanobacterium collected off the coast of Niihau, near Lehua Rock. The natural product exhibits anti-chemotactic activity at concentrations as low as 1.3 μM against Jurkat cells, cancerous T lymphocytes, and induces a G2/M phase cell cycle shift. Structural characterization of the natural product revealed the compound to be a 40-membered macrolactone with nine hydroxyl functional groups and a rare tert-butyl carbinol residue. PMID:26473885

  10. Spontaneous Electrical Activity and Spikes in the Tail of Marine Cercariae

    PubMed Central

    Tolstenkov, O. O.; Zhukovskaya, M. I.; Prokofiev, V. V.; Gustafsson, M. K. S.

    2013-01-01

    Spontaneous electrical activity is recorded in two species of marine cercariae, Cryptocotyle lingua and Himasthla elongata, with different types of swimming—by glass microelectrode recordings. Slow local field potentials (sLFPs) of low amplitude and fast high amplitude action potentials (APs) are found. The shape of the sLFPs is different in the species and correlates with the type of swimming. Fast high amplitude APs are recorded for the first time in cercariae. The limited number of APs included in the swimming pattern of larva suggests a key role for the spiking neurons in initiating the motility pattern in the cercaria and needs further research. PMID:27335850

  11. Macrolactone Nuiapolide, Isolated from a Hawaiian Marine Cyanobacterium, Exhibits Anti-Chemotactic Activity.

    PubMed

    Mori, Shogo; Williams, Howard; Cagle, Davey; Karanovich, Kristopher; Horgen, F David; Iii, Roger Smith; Watanabe, Coran M H

    2015-10-01

    A new bioactive macrolactone, nuiapolide (1) was identified from a marine cyanobacterium collected off the coast of Niihau, near Lehua Rock. The natural product exhibits anti-chemotactic activity at concentrations as low as 1.3 μM against Jurkat cells, cancerous T lymphocytes, and induces a G2/M phase cell cycle shift. Structural characterization of the natural product revealed the compound to be a 40-membered macrolactone with nine hydroxyl functional groups and a rare tert-butyl carbinol residue.

  12. 78 FR 6977 - Takes of Marine Mammals Incidental to Specified Activities; U.S. Navy Training and Testing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-31

    ... Part 218 Takes of Marine Mammals Incidental to Specified Activities; U.S. Navy Training and Testing... Incidental to Specified Activities; U.S. Navy Training and Testing Activities in the Hawaii-Southern... information. SUMMARY: NMFS has received a request from the U.S. Navy (Navy) for authorization to take...

  13. In-vitro antimicrobial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus

    PubMed Central

    Sathish, Kumar SR; Kokati, Venkata Bhaskara Rao

    2012-01-01

    Objective To investigate the antibacterial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus (MDRSA). Methods Fifty one actinobacterial strains were isolated from salt pans soil, costal area in Kothapattanam, Ongole, Andhra Pradesh. Primary screening was done using cross-streak method against MDRSA. The bioactive compounds are extracted from efficient actinobacteria using solvent extraction. The antimicrobial activity of crude and solvent extracts was performed using Kirby-Bauer method. MIC for ethyl acetate extract was determined by modified agar well diffusion method. The potent actinobacteria are identified using Nonomura key, Shirling and Gottlieb 1966 with Bergey's manual of determinative bacteriology. Results Among the fifty one isolates screened for antibacterial activity, SRB25 were found efficient against MDRSA. The ethyl acetate extracts showed high inhibition against test organism. MIC test was performed with the ethyl acetate extract against MDRSA and found to be 1 000 µg/mL. The isolated actinobacteria are identified as Streptomyces sp with the help of Nonomura key. Conclusions The current investigation reveals that the marine actinobacteria from salt pan environment can be able to produce new drug molecules against drug resistant microorganisms. PMID:23569848

  14. Post-coital contraceptive activity of crude extracts of Sri Lankan marine red algae.

    PubMed

    Ratnasooriya, W D; Premakumara, G A; Tillekeratne, L M

    1994-09-01

    This study investigates the potential of marine red algae as a source for post-coital contraceptive agents using three varieties: Gracilaria corticata, Gelidiella acerosa and Jania sp. Methanol: methylene chloride (1:1) extracts of these red algae were made and were orally administered (500 or 1000 mg/kg/day) to female rats from day 1 to day 7 of pregnancy. The higher dose of Gracilaria corticata and both doses of Gelidiella acerosa extracts produced significant post-coital contraceptive activities without any marked side effects. Furthermore, the post-coital contraceptive activity of the latter extract was dose-dependent. On the other hand, extract made from Jania sp. had no significant post-coital contraceptive action. The post-coital contraceptive activity of Gracilaria corticata was due to enhanced pre-implantation loss and of Gelidiella acerosa was due to elevated post-implantation loss. These findings indicate that marine red algae is a useful source to be harvested for potential post-coital contraceptive drugs. PMID:7805379

  15. Structure and activity of DmmA, a marine haloalkane dehalogenase

    SciTech Connect

    Gehret, Jennifer J.; Gu, Liangcai; Geders, Todd W.; Brown, William Clay; Gerwick, Lena; Gerwick, William H.; Sherman, David H.; Smith, Janet L.

    2012-08-01

    DmmA is a haloalkane dehalogenase (HLD) identified and characterized from the metagenomic DNA of a marine microbial consortium. Dehalogenase activity was detected with 1,3-dibromopropane as substrate, with steady-state kinetic parameters typical of HLDs (K{sub m} = 0.24 {+-} 0.05 mM, k{sub cat} = 2.4 {+-} 0.1 s{sup -1}). The 2.2-{angstrom} crystal structure of DmmA revealed a fold and active site similar to other HLDs, but with a substantially larger active site binding pocket, suggestive of an ability to act on bulky substrates. This enhanced cavity was shown to accept a range of linear and cyclic substrates, suggesting that DmmA will contribute to the expanding applications of HLDs.

  16. Development of a Low-energy Trigger for VERITAS

    SciTech Connect

    Kildea, J.

    2008-12-24

    During the 2007/2008 observing season a low-energy trigger configuration was developed and tested for VERITAS. The configuration makes uses of the small ({approx}35 m) baseline between two of the VERITAS telescopes and employs a much lower discriminator threshold and tighter coincidence window compared to the standard VERITAS trigger. Five hours of Crab Nebula ON/OFF observations were obtained in low-energy mode and were used to test new low-energy analysis algorithms. We present some details of the VERITAS low-energy trigger and the associated data analysis.

  17. Interaction between Low Energy Ions and the Complicated Organism

    NASA Astrophysics Data System (ADS)

    Yu, Zeng-liang

    1999-12-01

    Low energy ions exist widely in natural world, but people pay a little attention on the interaction between low energy ions and matter, it is even more out of the question of studying on the relation of low energy ions and the complicated organism. The discovery of bioeffect induced by ion implantation has, however, opened a new branch in the field of ion beam application in life sciences. This paper reports recent advances in research on the role of low energy ions in chemical synthesis of the biomolecules and application in genetic modification.

  18. Low-Energy Ballistic Transfers to Lunar Halo Orbits

    NASA Technical Reports Server (NTRS)

    Parker, Jeffrey S.

    2009-01-01

    Recent lunar missions have begun to take advantage of the benefits of low-energy ballistic transfers between the Earth and the Moon rather than implementing conventional Hohmann-like lunar transfers. Both Artemis and GRAIL plan to implement low-energy lunar transfers in the next few years. This paper explores the characteristics and potential applications of many different families of low-energy ballistic lunar transfers. The transfers presented here begin from a wide variety of different orbits at the Earth and follow several different distinct pathways to the Moon. This paper characterizes these pathways to identify desirable low-energy lunar transfers for future lunar missions.

  19. Tierra concrete homes: Low-energy residential building design

    SciTech Connect

    Hayter, S.J.; Torcellini, P.A.; Neimeyer, J.

    1997-12-31

    Using a whole building design concept, Tierra Concrete Homes, a home builder in Pueblo, Colorado, created low-energy, passive solar home designs. Passive solar features incorporated into the designs include house orientation, high-mass walls for thermal storage, exterior insulation, appropriate glazing type combined with overhangs to prevent summer overheating, open interior spaces to maximize daylighting potential, and high efficiency lighting. These ranch-style homes require no cooling and minimum heating equipment to maintain comfortable indoor conditions. They are economically competitive to build, consume little fossil fuel, and produce virtually no construction waste. This paper discusses how the design of one of these homes was optimized to further minimize energy consumption while maintaining an attractive livable environment. It also describes monitoring activities that are currently underway to verify predicted energy consumption.

  20. Low-energy electron collisions with thiophene

    NASA Astrophysics Data System (ADS)

    da Costa, R. F.; Varella, M. T. do N.; Lima, M. A. P.; Bettega, M. H. F.

    2013-05-01

    We report on elastic integral, momentum transfer, and differential cross sections for collisions of low-energy electrons with thiophene molecules. The scattering calculations presented here used the Schwinger multichannel method and were carried out in the static-exchange and static-exchange plus polarization approximations for energies ranging from 0.5 eV to 6 eV. We found shape resonances related to the formation of two long-lived π* anion states. These resonant structures are centered at the energies of 1.00 eV (2.85 eV) and 2.82 eV (5.00 eV) in the static-exchange plus polarization (static-exchange) approximation and belong to the B1 and A2 symmetries of the C2v point group, respectively. Our results also suggest the existence of a σ* shape resonance in the B2 symmetry with a strong d-wave character, located at around 2.78 eV (5.50 eV) as obtained in the static-exchange plus polarization (static-exchange) calculation. It is worth to mention that the results obtained at the static-exchange plus polarization level of approximation for the two π* resonances are in good agreement with the electron transmission spectroscopy results of 1.15 eV and 2.63 eV measured by Modelli and Burrow [J. Phys. Chem. A 108, 5721 (2004), 10.1021/jp048759a]. The existence of the σ* shape resonance is in agreement with the observations of Dezarnaud-Dandiney et al. [J. Phys. B 31, L497 (1998), 10.1088/0953-4075/31/11/004] based on the electron transmission spectra of dimethyl(poly)sulphides. A comparison among the resonances of thiophene with those of pyrrole and furan is also performed and, altogether, the resonance spectra obtained for these molecules point out that electron attachment to π* molecular orbitals is a general feature displayed by these five-membered heterocyclic compounds.

  1. Inhibitory Activity of Marine Sponge-Derived Natural Products against Parasitic Protozoa

    PubMed Central

    Orhan, Ilkay; Şener, Bilge; Kaiser, Marcel; Brun, Reto; Tasdemir, Deniz

    2010-01-01

    In this study, thirteen sponge-derived terpenoids, including five linear furanoterpenes: furospinulosin-1 (1), furospinulosin-2 (2), furospongin-1 (3), furospongin-4 (4), and demethylfurospongin-4 (5); four linear meroterpenes: 2-(hexaprenylmethyl)-2-methylchromenol (6), 4-hydroxy-3-octaprenylbenzoic acid (7), 4-hydroxy-3-tetraprenyl-phenylacetic acid (8), and heptaprenyl-p-quinol (9); a linear triterpene, squalene (10); two spongian-type diterpenes dorisenone D (11) and 11β-acetoxyspongi-12-en-16-one (12); a scalarane-type sesterterpene; 12-epi-deoxoscalarin (13), as well as an indole alkaloid, tryptophol (14) were screened for their in vitro activity against four parasitic protozoa; Trypanosoma brucei rhodesiense, Trypanosoma cruzi, Leishmania donovani and Plasmodium falciparum. Cytotoxic potential of the compounds on mammalian cells was also assessed. All compounds were active against T. brucei rhodesiense, with compound 8 being the most potent (IC50 0.60 μg/mL), whereas 9 and 12 were the most active compounds against T. cruzi, with IC50 values around 4 μg/mL. Compound 12 showed the strongest leishmanicidal activity (IC50 0.75 μg/mL), which was comparable to that of miltefosine (IC50 0.20 μg/mL). The best antiplasmodial effect was exerted by compound 11 (IC50 0.43 μg/mL), followed by compounds 7, 10, and 12 with IC50 values around 1 μg/mL. Compounds 9, 11 and 12 exhibited, besides their antiprotozoal activity, also some cytotoxicity, whereas all other compounds had low or no cytotoxicity towards the mammalian cell line. This is the first report of antiprotozoal activity of marine metabolites 1–14, and points out the potential of marine sponges in discovery of new antiprotozoal lead compounds. PMID:20161970

  2. An Aqueous Extract of Marine Microalgae Exhibits Antimetastatic Activity through Preferential Killing of Suspended Cancer Cells and Anticolony Forming Activity

    PubMed Central

    Somasekharan, Syam Prakash; El-Naggar, Amal; Sorensen, Poul H.

    2016-01-01

    Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma. In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells. In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted.

  3. An Aqueous Extract of Marine Microalgae Exhibits Antimetastatic Activity through Preferential Killing of Suspended Cancer Cells and Anticolony Forming Activity

    PubMed Central

    Somasekharan, Syam Prakash; El-Naggar, Amal; Sorensen, Poul H.

    2016-01-01

    Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma. In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells. In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted. PMID:27656243

  4. An Aqueous Extract of Marine Microalgae Exhibits Antimetastatic Activity through Preferential Killing of Suspended Cancer Cells and Anticolony Forming Activity.

    PubMed

    Somasekharan, Syam Prakash; El-Naggar, Amal; Sorensen, Poul H; Wang, Yuzhuo; Cheng, Hongwei

    2016-01-01

    Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma. In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells. In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted. PMID:27656243

  5. Low energy theorems in pion production

    SciTech Connect

    Holstein, B.R. |

    1992-09-01

    Considerable activity-both theoretical and experimental-has recently taken place involving the threshold and near threshold of pion photo- and electroproduction. This activity is herein summarized and a program for future work is outlined.

  6. Low energy theorems in pion production

    SciTech Connect

    Holstein, B.R. . Dept. of Physics and Astronomy Washington Univ., Seattle, WA . Inst. for Nuclear Theory)

    1992-01-01

    Considerable activity-both theoretical and experimental-has recently taken place involving the threshold and near threshold of pion photo- and electroproduction. This activity is herein summarized and a program for future work is outlined.

  7. Anthraquinones and Derivatives from Marine-Derived Fungi: Structural Diversity and Selected Biological Activities

    PubMed Central

    Fouillaud, Mireille; Venkatachalam, Mekala; Girard-Valenciennes, Emmanuelle; Caro, Yanis; Dufossé, Laurent

    2016-01-01

    Anthraquinones and their derivatives constitute a large group of quinoid compounds with about 700 molecules described. They are widespread in fungi and their chemical diversity and biological activities recently attracted attention of industries in such fields as pharmaceuticals, clothes dyeing, and food colorants. Their positive and/or negative effect(s) due to the 9,10-anthracenedione structure and its substituents are still not clearly understood and their potential roles or effects on human health are today strongly discussed among scientists. As marine microorganisms recently appeared as producers of an astonishing variety of structurally unique secondary metabolites, they may represent a promising resource for identifying new candidates for therapeutic drugs or daily additives. Within this review, we investigate the present knowledge about the anthraquinones and derivatives listed to date from marine-derived filamentous fungi′s productions. This overview highlights the molecules which have been identified in microorganisms for the first time. The structures and colors of the anthraquinoid compounds come along with the known roles of some molecules in the life of the organisms. Some specific biological activities are also described. This may help to open doors towards innovative natural substances. PMID:27023571

  8. Antiangiogenic activity of low-temperature lysozyme from a marine bacterium in vivo and in vitro

    NASA Astrophysics Data System (ADS)

    Wang, Zhenhua; Liu, Jincheng; Su, Ai; Sun, Mi; Wang, Chunbo

    2009-11-01

    We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 µg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca2+ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca2+ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.

  9. Anthraquinones and Derivatives from Marine-Derived Fungi: Structural Diversity and Selected Biological Activities.

    PubMed

    Fouillaud, Mireille; Venkatachalam, Mekala; Girard-Valenciennes, Emmanuelle; Caro, Yanis; Dufossé, Laurent

    2016-04-01

    Anthraquinones and their derivatives constitute a large group of quinoid compounds with about 700 molecules described. They are widespread in fungi and their chemical diversity and biological activities recently attracted attention of industries in such fields as pharmaceuticals, clothes dyeing, and food colorants. Their positive and/or negative effect(s) due to the 9,10-anthracenedione structure and its substituents are still not clearly understood and their potential roles or effects on human health are today strongly discussed among scientists. As marine microorganisms recently appeared as producers of an astonishing variety of structurally unique secondary metabolites, they may represent a promising resource for identifying new candidates for therapeutic drugs or daily additives. Within this review, we investigate the present knowledge about the anthraquinones and derivatives listed to date from marine-derived filamentous fungi's productions. This overview highlights the molecules which have been identified in microorganisms for the first time. The structures and colors of the anthraquinoid compounds come along with the known roles of some molecules in the life of the organisms. Some specific biological activities are also described. This may help to open doors towards innovative natural substances. PMID:27023571

  10. Total synthesis and biological activity of marine alkaloid Eudistomins Y1-Y7 and their analogues.

    PubMed

    Jin, Huijuan; Zhang, Puyong; Bijian, Krikor; Ren, Sumei; Wan, Shengbiao; Alaoui-Jamali, Moulay A; Jiang, Tao

    2013-05-01

    Eudistomin Y class compounds are a series of β-carbolines which was originally isolated from a marine turnicate or ascidian near the South Korea Sea. These compounds contain bromo-substituted groups, which is one of the typical characters of marine natural products. We report herein the chemical synthesis and biological evaluation of seven new β-carboline-based metabolites, Eudistomins Y1-Y7, and their hydroxyl-methylated phenyl derivatives. Using bromo-substituted tryptamines and bromo-substituted phenylglyoxals as the key intermediates, Eudistomins Y1-Y7 and their derivatives were synthesized via the acid-catalyzed Pictet-Spengler reaction and fully characterized by 1H- and 13C-NMR and mass spectroscopy. Biological studies revealed that all of the compounds showed moderate growth inhibitory activity against breast carcinoma cell line MDA-231 with IC50 of 15-63 μM and the inhibitory activities of hydroxyl-methylated phenyl products were higher than that of the corresponding natural products Eudistomins Y1-Y7.

  11. In vitro antioxidant and antimicrobial activities of two Hawaiian marine Limu: Ulva fasciata (Chlorophyta) and Gracilaria salicornia (Rhodophyta).

    PubMed

    Vijayavel, Kannappan; Martinez, Jonathan A

    2010-12-01

    The antioxidant and antimicrobial properties of two Hawaiian marine algae (Ulva fasciata and Gracilaria salicornia) were evaluated. Ethanolic extracts of these two algae exhibited intracellular reactive 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic radical, nitric oxide, superoxide radical, and hydroxyl radical scavenging activities. In addition to the antioxidant activity these algae possessed appreciable antimicrobial activity and total phenolic contents. The overall results have established that these two marine algae could be used to derive bioactive compounds for a possible role as nutraceutical agents.

  12. A Summary Comparison of Active Acoustic Detections and Visual Observations of Marine Mammals in the Canadian Beaufort Sea.

    PubMed

    Pyć, Cynthia D; Geoffroy, Maxime; Knudsen, Frank R

    2016-01-01

    Fisheries sonar was used to determine the applicability of active acoustic monitoring (AAM) for marine mammal detection in the Canadian Beaufort Sea. During 170 h of simultaneous observation by marine mammal observers and active acoustic observation, 119 Balaena mysticetus (bowheads) and 4 Delphinapterus leucas (belugas) were visually sighted, while 59 acoustic signals of bowheads were detected by AAM operators. Observations and detection of seals were also recorded. Comparative results indicate that commercially available active acoustic systems can detect seals at distances up to 500 m and large baleen whales at distances up to 2 km.

  13. A Summary Comparison of Active Acoustic Detections and Visual Observations of Marine Mammals in the Canadian Beaufort Sea.

    PubMed

    Pyć, Cynthia D; Geoffroy, Maxime; Knudsen, Frank R

    2016-01-01

    Fisheries sonar was used to determine the applicability of active acoustic monitoring (AAM) for marine mammal detection in the Canadian Beaufort Sea. During 170 h of simultaneous observation by marine mammal observers and active acoustic observation, 119 Balaena mysticetus (bowheads) and 4 Delphinapterus leucas (belugas) were visually sighted, while 59 acoustic signals of bowheads were detected by AAM operators. Observations and detection of seals were also recorded. Comparative results indicate that commercially available active acoustic systems can detect seals at distances up to 500 m and large baleen whales at distances up to 2 km. PMID:26611045

  14. Effects of dispersant and oil on survival and swimming activity in a marine copepod.

    PubMed

    Cohen, Jonathan H; McCormick, Lillian R; Burkhardt, Stephanie M

    2014-04-01

    Knowledge of lethal and sublethal effects of crude oil and dispersants on mesozooplankton are important to understanding ecosystem impacts of oil spills in marine environments. Here we (1) establish median lethal concentrations for water accommodated fractions of Corexit EC9500A dispersant, MC-252 crude oil (WAF), and dispersed crude oil (CEWAF) for the coastal copepod Labidocera aestiva, and (2) assess acute effects on L. aestiva swimming activity. Mortality assays with L. aestiva support that copepods are more sensitive than other zooplankton taxa to dispersant toxicity, while WAF and CEWAF are generally similar in their toxicity to this copepod species and other zooplankton. Acute effects on L. aestiva activity included impaired swimming upon WAF and CEWAF exposure. These results highlight that copepods are particularly sensitive to dispersant exposure, with acute effects on survival most evident with dispersant alone, and on swimming behavior when dispersant is mixed with crude oil. PMID:24402000

  15. α-Glucosidase inhibitory activity of marine sponges collected in Mauritius waters.

    PubMed

    Ramanjooloo, Avin; Cresteil, Thierry; Lebrasse, Cindy; Beedessee, Girish; Oogarah, Preeti; van Soest, Rob W M; Marie, Daniel E P

    2015-01-01

    This report describes the use of α-glucosidase to evaluate the anti-diabetic potential of extracts from marine sponges collected in the Mauritius waters. Initial screening at 1.0 mg/mL of 141 extracts obtained from 47 sponge species revealed 10 extracts with inhibitory activity greater than 85%. Seven of the 10 extracts were further tested at 0.1 and 0.01 mg/mL and only the methanol extract of two sponges namely Acanthostylotella sp. (ASSM) and Echinodictyum pykei (EPM) showed inhibition activity greater than 60% at 0.1 mg/mL with an IC50 value of 0.16 ± 0.02 and 0.04 ± 0.01 mg/mL, respectively, while being inactive at 0.01 mg/mL.

  16. Association of orogenic activity with the Ordovician radiation of marine life

    NASA Technical Reports Server (NTRS)

    Miller, A. I.; Mao, S.

    1995-01-01

    The Ordovician radiation of marine life was among the most substantial pulses of diversification in Earth history and coincided in time with a major increase in the global level of orogenic activity. To investigate a possible causal link between these two patterns, the geographic distributions of 6576 individual appearances of Ordovician vician genera around the world were evaluated with respect to their proximity to probable centers of orogeny (foreland basins). Results indicate that these genera, which belonged to an array of higher taxa that diversified in the Middle and Late Ordovician (trilobites, brachiopods, bivalves, gastropods, monoplacophorans), were far more diverse in, and adjacent to, foreland basins than they were in areas farther removed from orogenic activity (carbonate platforms). This suggests an association of orogeny with diversification at that time.

  17. The isolation and antioxidant activity of polysaccharides from the marine microalgae Isochrysis galbana.

    PubMed

    Sun, Yingying; Wang, Hui; Guo, Ganlin; Pu, Yinfang; Yan, Binlun

    2014-11-26

    Three polysaccharides, IPSI-A, IPSI-B and IPSII, were successfully isolated from the marine microalgae Isochrysis galbana through a combination of anion-exchange column chromatography and repeated gel chromatography. These three polysaccharides were demonstrated to have moderate scavenging activities against superoxide and hydroxyl radicals and moderate reductive power in a concentration-dependent manner. The IPSII demonstrated more effective antioxidant activities than IPSI-A and IPSI-B. IPSII had a molecular weight of 15.934 kDa belonging to a β-type heteropolysaccharide with a pyran group and primarily contained mannose with variable amounts of glucose, galactose and rhamnose based on an analysis of infrared spectroscopy (IR), electrospray ionization-mass spectrometry (ESI-MS) and (1)H nuclear magnetic resonance ((1)H NMR).

  18. Learning about Marine Biology. Superific Science Book VI. A Good Apple Science Activity Book for Grades 5-8+.

    ERIC Educational Resources Information Center

    Conway, Lorraine

    Based on the assumption that most students have a natural curiosity about the plant and animal life residing in the oceans, this document provides students in grades five through eight with activities in marine biology. The book provides illustrated information and learning activities dealing with: (1) diatoms; (2) the life cycle of the jellyfish;…

  19. Antibiofilm Activity of the Marine Bacterium Pseudoalteromonas sp. Strain 3J6▿

    PubMed Central

    Dheilly, Alexandra; Soum-Soutéra, Emmanuelle; Klein, Géraldine L.; Bazire, Alexis; Compère, Chantal; Haras, Dominique; Dufour, Alain

    2010-01-01

    Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and reduced bacterial attachment about 2-fold, which might partly explain the biofilm formation defect but not the loss of cell viability. SN3J6 had a wide spectrum of activity since it affected all Gram-negative marine strains tested except other Pseudoalteromonas strains. Biofilm biovolumes of the sensitive strains were reduced 3- to 530-fold, and the percentages of nonviable cells were increased 3- to 225-fold. Interestingly, SN3J6 also impaired biofilm formation by three strains belonging to the human-pathogenic species Pseudomonas aeruginosa, Salmonella enterica, and Escherichia coli. Such an antibiofilm activity is original and opens up a variety of applications for Pseudoalteromonas sp. 3J6 and/or its active exoproducts in biofilm prevention strategies. PMID:20363799

  20. Toward a harmonized approach for environmental assessment of human activities in the marine environment.

    PubMed

    Tamis, Jacqueline E; de Vries, Pepijn; Jongbloed, Ruud H; Lagerveld, Sander; Jak, Robbert G; Karman, Chris C; Van der Wal, Jan Tjalling; Slijkerman, Diana Me; Klok, Chris

    2016-10-01

    With a foreseen increase in maritime activities, and driven by new policies and conventions aiming at sustainable management of the marine ecosystem, spatial management at sea is of growing importance. Spatial management should ensure that the collective pressures caused by anthropogenic activities on the marine ecosystem are kept within acceptable levels. A multitude of approaches to environmental assessment are available to provide insight for sustainable management, and there is a need for a harmonized and integrated environmental assessment approach that can be used for different purposes and variable levels of detail. This article first provides an overview of the main types of environmental assessments: "environmental impact assessment" (EIA), "strategic environmental assessment" (SEA), "cumulative effect assessment" (CEA), and "environmental (or ecological) risk assessment" (ERA). Addressing the need for a conceptual "umbrella" for the fragmented approaches, a generic framework for environmental assessment is proposed: cumulative effects of offshore activities (CUMULEO). CUMULEO builds on the principle that activities cause pressures that may lead to adverse effects on the ecosystem. Basic elements and variables are defined that can be used consistently throughout sequential decision-making levels and diverse methodological implementations. This enables environmental assessment to start at a high strategic level (i.e., plan and/or program level), resulting in early environmental awareness and subsequently more informed, efficient, and focused project-level assessments, which has clear benefits for both industry and government. Its main strengths are simplicity, transparency, flexibility (allowing the use of both qualitative and quantitative data), and visualization, making it a powerful framework to support discussions with experts, stakeholders, and policymakers. Integr Environ Assess Manag 2016;12:632-642. © 2015 SETAC.

  1. What is a low-energy house and who cares?

    SciTech Connect

    Litt, B.R.

    1994-12-01

    Most energy analysts view low-energy houses as good things, yet differ in their expectations of what exactly a low energy house is. There are two intertwining threads to this report. The first is an evaluation of 50 buildings that have been claimed to be low-energy residences, for which monitored energy performance data have been collected. These data represent the preliminary effort in the ongoing update of the Buildings Energy-Use Compilation and Analysis (BECA) data base for new residences. The second thread concerns the definition of a low-energy house. After the elements of a definition are presented, their implications for actors involved in providing housing are identified. Several more tractable definitions are applied to the houses in this compilation. The outcomes illustrate ways in which different interests are served by various definitions. Different definitions can yield very different energy rankings. No single definition of a low-energy house is universally applicable.

  2. Antimicrobial Activity of Marine Bacterial Symbionts Retrieved from Shallow Water Hydrothermal Vents.

    PubMed

    Eythorsdottir, Arnheidur; Omarsdottir, Sesselja; Einarsson, Hjorleifur

    2016-06-01

    Marine sponges and other sessile macro-organisms were collected at a shallow water hydrothermal site in Eyjafjörður, Iceland. Bacteria were isolated from the organisms using selective media for actinomycetes, and the isolates were screened for antimicrobial activity. A total of 111 isolates revealed antimicrobial activity displaying different antimicrobial patterns which indicates production of various compounds. Known test strains were grown in the presence of ethyl acetate extracts from one selected isolate, and a clear growth inhibition of Staphylococcus aureus was observed down to 0.1 % extract concentration in the medium. Identification of isolates shows different species of Actinobacteria with Streptomyces sp. playing the largest role, but also members of Bacilli, Alphaproteobacteria and Gammaproteobacteria. Sponges have an excellent record regarding production of bioactive compounds, often involving microbial symbionts. At the hydrothermal vents, however, the majority of active isolates originated from other invertebrates such as sea anemones or algae. The results indicate that antimicrobial assays involving isolates in full growth can detect activity not visible by other methods. The macro-organisms inhabiting the Eyjafjörður hydrothermal vent area host diverse microbial species in the phylum Actinobacteria with antimicrobial activity, and the compounds responsible for the activity will be subject to further research. PMID:27147438

  3. The Marine-Derived Kinase Inhibitor Fascaplysin Exerts Anti-Thrombotic Activity

    PubMed Central

    Ampofo, Emmanuel; Später, Thomas; Müller, Isabelle; Eichler, Hermann; Menger, Michael D.; Laschke, Matthias W.

    2015-01-01

    Background: The marine-derived kinase inhibitor fascaplysin down-regulates the PI3K pathway in cancer cells. Since this pathway also plays an essential role in platelet signaling, we herein investigated the effect of fascaplysin on thrombosis. Methods: Fascaplysin effects on platelet activation, platelet aggregation and platelet-leukocyte aggregates (PLA) formation were analyzed by flow cytometry. Mouse dorsal skinfold chambers were used to determine in vivo the effect of fascaplysin on photochemically induced thrombus formation and tail-vein bleeding time. Results: Pre-treatment of platelets with fascaplysin reduced the activation of glycoprotein (GP)IIb/IIIa after protease-activated receptor-1-activating peptide (PAR-1-AP), adenosine diphosphate (ADP) and phorbol-12-myristate-13-acetate (PMA) stimulation, but did not markedly affect the expression of P-selectin. This was associated with a decreased platelet aggregation. Fascaplysin also decreased PLA formation after PMA but not PAR-1-AP and ADP stimulation. This may be explained by an increased expression of CD11b on leukocytes in PAR-1-AP- and ADP-treated whole blood. In the dorsal skinfold chamber model of photochemically induced thrombus formation, fascaplysin-treated mice revealed a significantly extended complete vessel occlusion time when compared to controls. Furthermore, fascaplysin increased the tail-vein bleeding time. Conclusion: Fascaplysin exerts anti-thrombotic activity, which represents a novel mode of action in the pleiotropic activity spectrum of this compound. PMID:26569265

  4. Sulfated Polysaccharides in Marine Sponges: Extraction Methods and Anti-HIV Activity

    PubMed Central

    Esteves, Ana I. S.; Nicolai, Marisa; Humanes, Madalena; Goncalves, Joao

    2011-01-01

    The extraction, fractionation and HIV-1 inhibition potential of polysaccharides extracted from three species of marine sponges, Erylus discophorus, Cliona celata and Stelletta sp., collected in the Northeastern Atlantic, is presented in this work. The anti-HIV activity of 23 polysaccharide pellets and three crude extracts was tested. Crude extracts prepared from Erylus discophorus specimens were all highly active against HIV-1 (90 to 95% inhibition). Cliona celata pellets showed low polysaccharide content (bellow 38.5%) and almost no anti-HIV activity (<10% inhibition). Stelletta sp. pellets, although quite rich in polysaccharide (up to 97.3%), showed only modest bioactivity (<36% HIV-1 inhibition). Erylus discophorus pellets were among the richest in terms of polysaccharide content (up to 98%) and the most active against HIV-1 (up to 95% inhibition). Chromatographic fractionation of the polysaccharide pellet obtained from a specimen of Erylus discophorus (B161) yielded only modestly active fractions. However, we could infer that the active molecule is most probably a high molecular weight sulfated polysaccharide (>2000 kDa), whose mechanism is possibly preventing viral attachment and entry (fusion inhibitor). PMID:21339952

  5. Antimicrobial Activity of Marine Bacterial Symbionts Retrieved from Shallow Water Hydrothermal Vents.

    PubMed

    Eythorsdottir, Arnheidur; Omarsdottir, Sesselja; Einarsson, Hjorleifur

    2016-06-01

    Marine sponges and other sessile macro-organisms were collected at a shallow water hydrothermal site in Eyjafjörður, Iceland. Bacteria were isolated from the organisms using selective media for actinomycetes, and the isolates were screened for antimicrobial activity. A total of 111 isolates revealed antimicrobial activity displaying different antimicrobial patterns which indicates production of various compounds. Known test strains were grown in the presence of ethyl acetate extracts from one selected isolate, and a clear growth inhibition of Staphylococcus aureus was observed down to 0.1 % extract concentration in the medium. Identification of isolates shows different species of Actinobacteria with Streptomyces sp. playing the largest role, but also members of Bacilli, Alphaproteobacteria and Gammaproteobacteria. Sponges have an excellent record regarding production of bioactive compounds, often involving microbial symbionts. At the hydrothermal vents, however, the majority of active isolates originated from other invertebrates such as sea anemones or algae. The results indicate that antimicrobial assays involving isolates in full growth can detect activity not visible by other methods. The macro-organisms inhabiting the Eyjafjörður hydrothermal vent area host diverse microbial species in the phylum Actinobacteria with antimicrobial activity, and the compounds responsible for the activity will be subject to further research.

  6. Marine pharmacology in 2009-2011: marine compounds with antibacterial, antidiabetic, antifungal, anti-inflammatory, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous systems, and other miscellaneous mechanisms of action.

    PubMed

    Mayer, Alejandro M S; Rodríguez, Abimael D; Taglialatela-Scafati, Orazio; Fusetani, Nobuhiro

    2013-07-16

    The peer-reviewed marine pharmacology literature from 2009 to 2011 is presented in this review, following the format used in the 1998-2008 reviews of this series. The pharmacology of structurally-characterized compounds isolated from marine animals, algae, fungi and bacteria is discussed in a comprehensive manner. Antibacterial, antifungal, antiprotozoal, antituberculosis, and antiviral pharmacological activities were reported for 102 marine natural products. Additionally, 60 marine compounds were observed to affect the immune and nervous system as well as possess antidiabetic and anti-inflammatory effects. Finally, 68 marine metabolites were shown to interact with a variety of receptors and molecular targets, and thus will probably contribute to multiple pharmacological classes upon further mechanism of action studies. Marine pharmacology during 2009-2011 remained a global enterprise, with researchers from 35 countries, and the United States, contributing to the preclinical pharmacology of 262 marine compounds which are part of the preclinical pharmaceutical pipeline. Continued pharmacological research with marine natural products will contribute to enhance the marine pharmaceutical clinical pipeline, which in 2013 consisted of 17 marine natural products, analogs or derivatives targeting a limited number of disease categories.

  7. Marine Pharmacology in 2009–2011: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis, and Antiviral Activities; Affecting the Immune and Nervous Systems, and other Miscellaneous Mechanisms of Action †

    PubMed Central

    Mayer, Alejandro M. S.; Rodríguez, Abimael D.; Taglialatela-Scafati, Orazio; Fusetani, Nobuhiro

    2013-01-01

    The peer-reviewed marine pharmacology literature from 2009 to 2011 is presented in this review, following the format used in the 1998–2008 reviews of this series. The pharmacology of structurally-characterized compounds isolated from marine animals, algae, fungi and bacteria is discussed in a comprehensive manner. Antibacterial, antifungal, antiprotozoal, antituberculosis, and antiviral pharmacological activities were reported for 102 marine natural products. Additionally, 60 marine compounds were observed to affect the immune and nervous system as well as possess antidiabetic and anti-inflammatory effects. Finally, 68 marine metabolites were shown to interact with a variety of receptors and molecular targets, and thus will probably contribute to multiple pharmacological classes upon further mechanism of action studies. Marine pharmacology during 2009–2011 remained a global enterprise, with researchers from 35 countries, and the United States, contributing to the preclinical pharmacology of 262 marine compounds which are part of the preclinical pharmaceutical pipeline. Continued pharmacological research with marine natural products will contribute to enhance the marine pharmaceutical clinical pipeline, which in 2013 consisted of 17 marine natural products, analogs or derivatives targeting a limited number of disease categories. PMID:23880931

  8. Habitat segregation and biochemical activities of marine members of the family vibrionaceae.

    PubMed

    Simidu, U; Tsukamoto, K

    1985-10-01

    A comparative study of marine members of the family Vibrionaceae with the technique of numerical taxonomy revealed habitat segregation as well as a cosmopolitan nature of species distribution among the vibrios in different marine environments. The bacterial strains analyzed were isolated from seawater, sediments, phyto- and zooplankton, and fish in the Indian Ocean, the South and East China Sea, and West Pacific Ocean, and coastal areas of Japan. A total of 155 morphological, physiological, and biochemical tests were carried out for each of 405 strains examined. The results showed that most of the large taxonomical clusters which emerged from the computation corresponded to ecological groups which have particular niches. For instance, each group of seawater vibrios inhabited a particular water layer of limited depth range, in spite of the fact that strains of the group were isolated from sampling locations spread over a wide area from the Indian Ocean to Japanese coast. Various vibrio groups showed remarkable differences in their physiological and biochemical activities, and the activities of each group seemed to correspond with its ecological niche. The strains which inhabited surface-water layers grew fast and actively utilized many high-molecular-weight organic compounds and carbohydrates that are derived from fresh, easily degradable organic matter present in the surface waters, whereas the middle- and deep-water vibrios did not decompose most of the high-molecular-weight organic compounds except chitin but, rather, utilized some carbohydrates and organic acids which seemed to be derived from refractory particulate organic matter present in the deeper waters.

  9. Marine pharmacology in 2005–6: Marine Compounds with Anthelmintic, Antibacterial, Anticoagulant, Antifungal, Anti-inflammatory, Antimalarial, Antiprotozoal, Antituberculosis, and Antiviral Activities; affecting the Cardiovascular, Immune and Nervous Systems, and other Miscellaneous Mechanisms of Action

    PubMed Central

    Mayer, Alejandro M. S.; Rodriguez, Abimael D.; Berlinck, Roberto G. S.; Hamann, Mark T.

    2009-01-01

    BACKGROUND The review presents the 2005–2006 peer-reviewed marine pharmacology literature, and follows a similar format to the authors’ 1998–2004 reviews. The preclinical pharmacology of chemically characterized marine compounds isolated from marine animals, algae, fungi and bacteria is systematically presented. RESULTS Anthelminthic, antibacterial, anticoagulant, antifungal, antimalarial, antiprotozoal, antituberculosis and antiviral activities were reported for 78 marine chemicals. Additionally 47 marine compounds were reported to affect the cardiovascular, immune and nervous system as well as possess anti-inflammatory effects. Finally, 58 marine compounds were shown to bind to a variety of molecular targets, and thus could potentially contribute to several pharmacological classes. CONCLUSIONS Marine pharmacology research during 2005–2006 was truly global in nature, involving investigators from 32 countries, and the United States, and contributed 183 marine chemical leads to the research pipeline aimed at the discovery of novel therapeutic agents. SIGNIFICANCE Continued preclinical and clinical research with marine natural products demonstrating a broad spectrum of pharmacological activity and will probably result in novel therapeutic agents for the treatment of multiple disease categories. PMID:19303911

  10. Formation of marine snow and enhanced enzymatic activities in oil-contaminated seawater

    NASA Astrophysics Data System (ADS)

    Ziervogel, K.; McKay, L.; Yang, T.; Rhodes, B.; Nigro, L.; Gutierrez, T.; Teske, A.; Arnosti, C.

    2010-12-01

    The fate of oil spilled into the ocean depends on its composition, as well as on biological, chemical, and physical characteristics of the spill site. We investigated the effects of oil addition from the Deepwater Horizon (DH) spill on otherwise uncontaminated water collected close to the spill site. Incubation on a roller table mimicked the physical dynamics of natural seawater, leading to the formation of marine snow-oil aggregates. We measured the enzymatic activities of heterotrophic microbes associated with the aggregates and in the surrounding water, and assessed microbial population and community composition as oil-marine snow aggregates formed and aged in the water. Surface seawater taken near the spill site in May 2010 that had no visible crude oil was incubated in 1-l glass bottles with (oil-bottles) and without (no-oil bottles) a seawater-oil mixture collected from the same site. In the oil-bottles formation of brownish, densely packed marine snow (2-3 cm diameter) was observed within the first hour of the roller table incubation. In contrast no-oil bottles showed aggregate formation only after 3 days, and aggregates were almost transparent, less abundant, and smaller in size (< 1cm diameter). Subsamples of the water surrounding the aggregates were taken throughout 21 days of the roller table incubation, and analyzed for bacterial abundance and community structure as well as the activities of hydrolytic enzymes that are used by heterotrophic bacteria to degrade organic matter. We monitored oil-degrading activities with MUF-stearate and -butyrate, and also measured b-glucosidase, alkaline phosphatase, aminopeptidase, and six different polysaccharide hydrolase activities. Enzymatic activities were up to one order of magnitude higher in the oil-bottles compared with the no-oil bottles throughout the entire incubation time. Butyrate hydrolysis was elevated throughout the time course of the incubation, and stearate hydrolysis was particularly high over the

  11. Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years

    PubMed Central

    Wang, Lingchong; Wang, Xiangyu; Wu, Hao; Liu, Rui

    2014-01-01

    Among the three main divisions of marine macroalgae (Chlorophyta, Phaeophyta and Rhodophyta), marine green algae are valuable sources of structurally diverse bioactive compounds and remain largely unexploited in nutraceutical and pharmaceutical areas. Recently, a great deal of interest has been developed to isolate novel sulfated polysaccharides (SPs) from marine green algae because of their numerous health beneficial effects. Green seaweeds are known to synthesize large quantities of SPs and are well established sources of these particularly interesting molecules such as ulvans from Ulva and Enteromorpha, sulfated rhamnans from Monostroma, sulfated arabinogalactans from Codium, sulfated galacotans from Caulerpa, and some special sulfated mannans from different species. These SPs exhibit many beneficial biological activities such as anticoagulant, antiviral, antioxidative, antitumor, immunomodulating, antihyperlipidemic and antihepatotoxic activities. Therefore, marine algae derived SPs have great potential for further development as healthy food and medical products. The present review focuses on SPs derived from marine green algae and presents an overview of the recent progress of determinations of their structural types and biological activities, especially their potential health benefits. PMID:25257786

  12. Triclosan causes toxic effects to algae in marine biofilms, but does not inhibit the metabolic activity of marine biofilm bacteria.

    PubMed

    Johansson, C Henrik; Janmar, Lisa; Backhaus, Thomas

    2014-07-15

    Effects of the antimicrobial agent triclosan to natural periphyton communities (biofilms, comprising primarily microalgae and bacteria) were assessed in two independent experiments during spring and summer. For that purpose a semi-static test system was used in which periphyton was exposed to a concentration range of 5-9054 nmol/L triclosan. Effects on algae were analyzed as content and composition of photosynthetic pigments. The corresponding EC50 values were 39.25 and 302.45 nmol/L for the spring and summer experiment, respectively. Effects on periphytic bacteria were assessed as effects on carbon utilization patterns, using Biolog Ecoplates. No inhibition of either total carbon utilization or functional diversity was observed, indicating a pronounced triclosan tolerance of the marine bacteria. In contrast, a small stimulation of the total carbon utilization was observed at triclosan concentrations exceeding 100 nmol/L.

  13. Microbial activity in deep marine sediments: does pressure make the difference?

    NASA Astrophysics Data System (ADS)

    Picard, Aude; Ferdelman, Timothy G.

    2012-07-01

    We attempted to evaluate the effects of high hydrostatic pressure on microbial heterotrophic activity in deep marine sediments from the Atlantic Ocean. We investigated the potential respiration rates (acetate/glucose oxidation to CO2) in oxic sediments recovered from up to ~4500 m water depth. Incubations were performed at ambient pressure and at near in situ pressure (~40-45 MPa) with sediments stored at ambient pressure and at in situ pressure. Potential respiration rates in sediments stored at ambient pressure were lower when measured at in situ pressure than when measured at ambient pressure, independently of the substrate used. It appears that the pressure of storage is critical since potential respiration rates of sediments stored at in situ pressure were higher than in the counterpart sediments stored at ambient pressure.

  14. Antipodal crambescin A2 homologues from the marine sponge Pseudaxinella reticulata. Antifungal structure-activity relationships.

    PubMed

    Jamison, Matthew T; Molinski, Tadeusz F

    2015-03-27

    Investigation of antifungal natural products from the marine sponge Pseudaxinella reticulata from the Bahamas led to the discovery of new crambescin homologues (1, 2) and enantiomers (3, 4) of known natural products. The cyclic-guanidine structures were solved through analysis of 2D NMR, MS-MS, and CD data. The absolute configurations of 1-4 were established as 13R-opposite of known homologues reported from Crambe crambe obtained from the Mediterranean Sea-by comparison of their CD spectra with predicted Cotton effects obtained from DFT calculations. Antifungal activities of 1-4 against the pathogenic strains Candida albicans and Cryptococcus sp. were observed to correlate potency (MIC50 and MIC90) with the length of the alkyl side chain. PMID:25738226

  15. Sodium Copper Chlorophyllin Immobilization onto Hippospongia communis Marine Demosponge Skeleton and Its Antibacterial Activity

    PubMed Central

    Norman, Małgorzata; Bartczak, Przemysław; Zdarta, Jakub; Tomala, Wiktor; Żurańska, Barbara; Dobrowolska, Anna; Piasecki, Adam; Czaczyk, Katarzyna; Ehrlich, Hermann; Jesionowski, Teofil

    2016-01-01

    In this study, Hippospongia communis marine demosponge skeleton was used as an adsorbent for sodium copper chlorophyllin (SCC). Obtained results indicate the high sorption capacity of this biomaterial with respect to SCC. Batch experiments were performed under different conditions and kinetic and isotherms properties were investigated. Acidic pH and the addition of sodium chloride increased SCC adsorption. The experimental data were well described by a pseudo-second order kinetic model. Equilibrium adsorption isotherms were determined and the experimental data were analyzed using both Langmuir and Freundlich isotherms. The effectiveness of the process was confirmed by 13C Cross Polarization Magic Angle Spinning Nuclear Magnetic Resonance (13C CP/MAS NMR), Fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDS) and thermogravimetric analysis (TG). This novel SCC-sponge-based functional hybrid material was found to exhibit antimicrobial activity against the gram-positive bacterium Staphylococcus aureus. PMID:27690001

  16. Diel activity and variability in habitat use of white sea bream in a temperate marine protected area.

    PubMed

    Di Lorenzo, Manfredi; Fernández, Tomás Vega; Badalamenti, Fabio; Guidetti, Paolo; Starr, Richard M; Giacalone, Vincenzo Maximiliano; Di Franco, Antonio; D'Anna, Giovanni

    2016-05-01

    Fish populations are often comprised of individuals that use habitats and associated resources in different ways. We placed sonic transmitters in, and tracked movements of, white sea bream (Diplodus sargus sargus) in the no-take zone of a Mediterranean marine protected area: the Torre Guaceto marine protected area, (Adriatic Sea, Italy). Tagged fish displayed three types of diel activity patterns in three different habitats: sand, rocky reefs and "matte" of the seagrass Posidonia oceanica. Individuals were more active during the day than at night. Overall, white sea bream displayed a remarkable behavioural plasticity in habitat use. Our results indicate that the observed behavioural plasticity in the marine protected area could be the result of multiple ecological and environmental drivers such as size, sex and increased intra-specific competition. Our findings support the view that habitat diversity helps support high densities of fishes. PMID:26922044

  17. Three peroxisome proliferator-activated receptor isotypes from each of two species of marine fish.

    PubMed

    Leaver, Michael J; Boukouvala, Evridiki; Antonopoulou, Efthimia; Diez, Amalia; Favre-Krey, Laurence; Ezaz, M Tariq; Bautista, José M; Tocher, Douglas R; Krey, Grigorios

    2005-07-01

    The cloning and characterization of cDNAs and genes encoding three peroxisome proliferator-activated receptor (PPAR) isotypes from two species of marine fish, the plaice (Pleuronectes platessa) and the gilthead sea bream (Sparus aurata), are reported for the first time. Although differences in the genomic organization of the fish PPAR genes compared with their mammalian counterparts are evident, sequence alignments and phylogenetic comparisons show the fish genes to be homologs of mammalian PPARalpha, PPARbeta/delta, and PPARgamma. Like their mammalian homologs, fish PPARs bind to a variety of natural PPAR response elements (PPREs) present in the promoters of mammalian or piscine genes. In contrast, the mRNA expression pattern of PPARs in the two fish species differs from that observed in other vertebrates. Thus, PPARgamma is expressed more widely in fish tissues than in mammals, whereas PPARalpha and beta are expressed similarly in profile to mammals. Furthermore, nutritional status strongly influences the expression of all three PPAR isotypes in liver, whereas it has no effect on PPAR expression in intestinal and adipose tissues. Fish PPARalpha and beta exhibit an activation profile similar to that of the mammalian PPAR in response to a variety of activators/ligands, whereas PPARgamma is not activated by mammalian PPARgamma-specific ligands. Amino acid residues shown to be critical for ligand binding in mammalian PPARs are not conserved in fish PPARgamma and therefore, together with the distinct tissue expression profile of this receptor, suggest potential differences in the function of PPARgamma in fish compared with mammals.

  18. Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine Diatoms

    PubMed Central

    Nguyen-Deroche, Thi Le Nhung; Caruso, Aurore; Le, Thi Trung; Bui, Trang Viet; Schoefs, Benoît; Tremblin, Gérard; Morant-Manceau, Annick

    2012-01-01

    Zinc-supplementation (20 μM) effects on growth, photosynthesis, antioxidant enzyme activities (superoxide dismutase, ascorbate peroxidase, catalase), and the expression of phytochelatin synthase gene were investigated in four marine diatoms (Amphora acutiuscula, Nitzschia palea, Amphora coffeaeformis and Entomoneis paludosa). Zn-supplementation reduced the maximum cell density. A linear relationship was found between the evolution of gross photosynthesis and total chlorophyll content. The Zn treatment decreased the electron transport rate except in A. coffeaeformis and in E. paludosa at high irradiance. A linear relationship was found between the efficiency of light to evolve oxygen and the size of the light-harvesting antenna. The external carbonic anhydrase activity was stimulated in Zn-supplemented E. paludosa but was not correlated with an increase of photosynthesis. The total activity of the antioxidant enzymes did not display any clear increase except in ascorbate peroxidase activity in N. palea. The phytochelatin synthase gene was identified in the four diatoms, but its expression was only revealed in N. palea, without a clear difference between control and Zn-supplemented cells. Among the four species, A. paludosa was the most sensitive and A. coffeaeformis, the most tolerant. A. acutiuscula seemed to be under metal starvation, whereas, to survive, only N. palea developed several stress responses. PMID:22645501

  19. Penicillium chrysogenum DSOA associated with marine sponge (Tedania anhelans) exhibit antimycobacterial activity.

    PubMed

    Visamsetti, Amarendra; Ramachandran, Santhosh Sarojini; Kandasamy, Dhevendaran

    2016-04-01

    A strain of Penicillium chrysogenum was isolated from Tedania anhelans (marine sponge) collected from Indian Ocean (8°22'30″N latitude and 76°59'16″ longitude) and deposited in culture collection centers. The strain subjected to different culture conditions for production of extrolites were extracted using ethyl acetate and chloroform. When both extracts were subjected for antibacterial activity, latter had high activity. Minimum inhibitory concentration of chloroform extract ranged from 31.25-1000 μg/mL in tested microbes such as, Mycobacterium tuberculosis H37Ra, Mycobacterium avium, Mycobacterium fortuitum, Mycobacterium smegmatis, Mycobacterium vaccae, Staphylococcus aureus, Aeromonas hydrophila, Pseudomonas aeruginosa and Vibrio cholerae. No cytotoxicity was observed in Vero cell line up to 399.10 μg/mL. Antibacterial activity previously reported by Parameswaran et al. in 1997 from ethyl acetate extract of T. anhelans might be due to the diketopiperazines, Cyclo-(L-Pro-L-Phe) and Cyclo-(L-Leu-L-Pro) produced by the associated fungi-P. chrysogenum DSOA. It is producing a metabolites having antimycobacterial activity, a first report. PMID:26717859

  20. Interactions between Carotenoids from Marine Bacteria and Other Micronutrients: Impact on Stability and Antioxidant Activity.

    PubMed

    Sy, Charlotte; Dangles, Olivier; Borel, Patrick; Caris-Veyrat, Catherine

    2015-11-19

    Recently isolated spore-forming pigmented marine bacteria Bacillus indicus HU36 are sources of oxygenated carotenoids with original structures (about fifteen distinct yellow and orange pigments with acylated d-glucosyl groups). In this study, we evaluated the stability (sensitivity to iron-induced autoxidation) and antioxidant activity (inhibition of iron-induced lipid peroxidation) of combinations of bacterial HU36 carotenoids with the bacterial vitamin menaquinone MQ-7 and with phenolic antioxidants (vitamin E, chlorogenic acid, rutin). Unexpectedly, MQ-7 strongly improves the ability of HU36 carotenoids to inhibit Fe(II)-induced lipid peroxidation, although MQ-7 was not consumed in the medium. We propose that their interaction modifies the carotenoid antioxidant mechanism(s), possibly by allowing carotenoids to scavenge the initiating radicals. For comparison, β-carotene and lycopene in combination were shown to exhibit a slightly higher stability toward iron-induced autoxidation, as well as an additive antioxidant activity as compared to the carotenoids, individually. HU36 carotenoids and phenolic antioxidants displayed synergistic activities in the inhibition of linoleic acid peroxidation induced by heme iron, but not by free iron. Synergism could arise from antioxidants interacting via electron transfer through the porphyrin nucleus of heme iron. Overall, combining antioxidants acting via complementary mechanisms could be the key for optimizing the activity of this bacterial carotenoid cocktail.

  1. Amy63, a novel type of marine bacterial multifunctional enzyme possessing amylase, agarase and carrageenase activities

    PubMed Central

    Liu, Ge; Wu, Shimei; Jin, Weihua; Sun, Chaomin

    2016-01-01

    A multifunctional enzyme is one that performs multiple physiological functions, thus benefiting the organism. Characterization of multifunctional enzymes is important for researchers to understand how organisms adapt to different environmental challenges. In the present study, we report the discovery of a novel multifunctional enzyme Amy63 produced by marine bacterium Vibrio alginolyticus 63. Remarkably, Amy63 possesses amylase, agarase and carrageenase activities. Amy63 is a substrate promiscuous α-amylase, with the substrate priority order of starch, carrageenan and agar. Amy63 maintains considerable amylase, carrageenase and agarase activities and stabilities at wide temperature and pH ranges, and optimum activities are detected at temperature of 60 °C and pH of 6.0, respectively. Moreover, the heteroexpression of Amy63 dramatically enhances the ability of E. coli to degrade starch, carrageenan and agar. Motif searching shows three continuous glycosyl hydrolase 70 (GH70) family homologs existed in Amy63 encoding sequence. Combining serial deletions and phylogenetic analysis of Amy63, the GH70 homologs are proposed as the determinants of enzyme promiscuity. Notably, such enzymes exist in all kingdoms of life, thus providing an expanded perspective on studies of multifunctional enzymes. To our knowledge, this is the first report of an amylase having additional agarase and carrageenase activities. PMID:26725302

  2. Antimicrobial Activity of Euplotin C, the Sesquiterpene Taxonomic Marker from the Marine Ciliate Euplotes crassus

    PubMed Central

    Savoia, Dianella; Avanzini, Claudio; Allice, Tiziano; Callone, Emanuela; Guella, Graziano; Dini, Fernando

    2004-01-01

    Strains of the marine ciliate protist Euplotes crassus produce exclusive terpenoids called euplotins that play an ecological role. Among these derivatives, euplotin C is the main of four secondary metabolites isolated from cultures of this protozoon and represents the sesquiterpene taxonomic marker from E. crassus. Because different terpenoid metabolites of plant origin showed a certain antimicrobial activity, we assessed the compound euplotin C, purified by high-pressure liquid chromatography and solubilized in two solubility enhancers, against the protozoa Leishmania major and Leishmani infantum, the fungus Candida albicans, and nine strains of gram-positive and gram-negative microorganisms. An activity of euplotin C against Leishmania promastigotes was demonstrated (50% lethal doses were 4.6 or 8.1 μg/ml depending on the agent used to solubilize the compound), while the effect was less evident on Candida and nearly absent on bacteria. A nonsignificant cytotoxicity (50% lethal dose, >200 μg/ml) against the J774 cell line was observed. A leishmanicidal activity was also shown by the living, euplotin-producing cells of E. crassus cultured together with promastigotes; this activity increased with time from 10 min to 6 h of incubation. This study provides an initial rationale for the evaluation of euplotin C and other similar natural products as alternative or possibly synergistic compounds for current antiprotozoon chemotherapeutics. PMID:15388442

  3. Interactions between Carotenoids from Marine Bacteria and Other Micronutrients: Impact on Stability and Antioxidant Activity

    PubMed Central

    Sy, Charlotte; Dangles, Olivier; Borel, Patrick; Caris-Veyrat, Catherine

    2015-01-01

    Recently isolated spore-forming pigmented marine bacteria Bacillus indicus HU36 are sources of oxygenated carotenoids with original structures (about fifteen distinct yellow and orange pigments with acylated d-glucosyl groups). In this study, we evaluated the stability (sensitivity to iron-induced autoxidation) and antioxidant activity (inhibition of iron-induced lipid peroxidation) of combinations of bacterial HU36 carotenoids with the bacterial vitamin menaquinone MQ-7 and with phenolic antioxidants (vitamin E, chlorogenic acid, rutin). Unexpectedly, MQ-7 strongly improves the ability of HU36 carotenoids to inhibit FeII-induced lipid peroxidation, although MQ-7 was not consumed in the medium. We propose that their interaction modifies the carotenoid antioxidant mechanism(s), possibly by allowing carotenoids to scavenge the initiating radicals. For comparison, β-carotene and lycopene in combination were shown to exhibit a slightly higher stability toward iron-induced autoxidation, as well as an additive antioxidant activity as compared to the carotenoids, individually. HU36 carotenoids and phenolic antioxidants displayed synergistic activities in the inhibition of linoleic acid peroxidation induced by heme iron, but not by free iron. Synergism could arise from antioxidants interacting via electron transfer through the porphyrin nucleus of heme iron. Overall, combining antioxidants acting via complementary mechanisms could be the key for optimizing the activity of this bacterial carotenoid cocktail. PMID:26610529

  4. 76 FR 33721 - Takes of Marine Mammals Incidental to Specified Activities; Harbor Activities Related to the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... 3, 2010 (74 FR 46742, September 11, 2009). The Delta IV/EELV launch vehicle is comprised of a common... activities). NMFS outlined the purpose of the program in the Notice of Proposed IHA (76 FR 21862, April 19, 2011). The activities to be conducted have not changed between the Notice of Proposed IHA (76 FR...

  5. 76 FR 21862 - Takes of Marine Mammals Incidental to Specified Activities; Harbor Activities Related to the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-19

    ... not limited to, migration, breathing, nursing, breeding, feeding, or sheltering . Summary of Request... support Delta IV/EELV launch activities from the Space Launch Complex at VAFB Harbor and would occur in... the same activities from 2002 to 2010, with the last IHA expiring on September 3, 2010 (74 FR...

  6. Parity violation in low-energy neutron-deuteron scattering

    SciTech Connect

    Song, Young-Ho; Gudkov, Vladimir; Lazauskas, Rimantas

    2011-01-15

    Parity-violating effects for low-energy elastic neutron deuteron scattering are calculated for Desplanques, Donoghue, and Holstein (DDH) and effective field theory types of weak potentials in a distorted-wave Born approximation, using realistic hadronic strong interaction wave functions, obtained by solving three-body Faddeev equations in configuration space. The resulting relation between physical observables and low-energy constants can be used to fix low-energy constants from experiments. Potential model dependencies of parity-violating effects are discussed.

  7. Use of low energy hydrogen ion implants in high efficiency crystalline silicon solar cells

    NASA Technical Reports Server (NTRS)

    Fonash, S. J.; Singh, R.

    1985-01-01

    This program is a study of the use of low energy hydrogen ion implantation for high efficiency crystalline silicon solar cells. The first quarterly report focuses on two tasks of this program: (1) an examination of the effects of low energy hydrogen implants on surface recombination speed; and (2) an examination of the effects of hydrogen on silicon regrowth and diffusion in silicon. The first part of the project focussed on the measurement of surface properties of hydrogen implanted silicon. Low energy hydrogen ions when bombarded on the silicon surface will create structural damage at the surface, deactivate dopants and introduce recombination centers. At the same time the electrically active centers such as dangling bonds will be passivated by these hydrogen ions. Thus hydrogen is expected to alter properties such as the surface recombination velocity, dopant profiles on the emitter, etc. In this report the surface recombination velocity of a hydrogen emplanted emitter was measured.

  8. Synthesis and structure-activity relationships of the halovirs, antiviral natural products from a marine-derived fungus.

    PubMed

    Rowley, David C; Kelly, Sara; Jensen, Paul; Fenical, William

    2004-09-15

    The halovirs are linear, lipophilic peptides produced by a marine-derived fungus of the genus Scytalidium. We recently reported that these molecules possess potent in vitro activity against the herpes simplex viruses 1 and 2. Here we present structure-activity relationships defining key structural elements for optimal viral inhibition. Results demonstrate that an N(alpha)-acyl chain of at least 14 carbons and an Aib-Pro dipeptide are critical for maintaining the antiviral activity.

  9. Sleep inducing effect of low energy emission therapy.

    PubMed

    Reite, M; Higgs, L; Lebet, J P; Barbault, A; Rossel, C; Kuster, N; Dafni, U; Amato, D; Pasche, B

    1994-01-01

    The sleep inducing effect of a 15 min treatment with either an active or an inactive Low Energy Emission Therapy (LEET) device emitting amplitude-modulated electromagnetic (EM) fields was investigated in a double-blind cross-over study performed on 52 healthy subjects. All subjects were exposed to both active and inactive LEET treatment sessions, with an interval of at least 1 week between the two sessions. LEET consists of 27.12 MHz amplitude-modulated (sine wave) EM fields emitted intrabuccally by means of an electrically conducting mouthpiece in direct contact with the oral mucosa. The estimated local peak SAR is less than 10 W/kg in the oral mucosa and 0.1 to 100 mW/kg in brain tissue. No appreciable sensation is experienced during treatment, and subjects are therefore unable to tell whether they are receiving an active or an inactive treatment. In this study the active treatment consisted of EM fields intermittently amplitude-modulated (sine wave) at 42.7 Hz for 3 s followed by a pause of 1 s during which no EM fields were emitted. During the inactive treatment no EM fields were emitted. Baseline EEGs were obtained and 15 min post-treatment EEGs were recorded and analyzed according to the Loomis classification. A significant decrease (paired t test) in sleep latency to stage B2 (-1.78 +/- 5.57 min, P = 0.013), and an increase in the total duration of stage B2 (1.15 +/- 2.47 min, P = 0.0008) were observed on active treatment as compared with inactive treatment.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:8155071

  10. Low Energy Nuclear Reaction Products at Surfaces

    NASA Astrophysics Data System (ADS)

    Nagel, David J.

    2008-03-01

    This paper examines the evidence for LENR occurring on or very near to the surface of materials. Several types of experimental indications for LENR surface reactions have been reported and will be reviewed. LENR result in two types of products, energy and the appearance of new elements. The level of instantaneous power production can be written as the product of four factors: (1) the total area of the surface on which the reactions can occur, (2) the fraction of the area that is active at any time, (3) the reaction rate, that is, the number of reactions per unit active area per second, and (4) the energy produced per reaction. Each of these factors, and their limits, are reviewed. A graphical means of relating these four factors over their wide variations has been devised. The instantaneous generation of atoms of new elements can also be written as the product of the first three factors and the new elemental mass produced per reaction. Again, a graphical means of presenting the factors and their results over many orders of magnitude has been developed.

  11. Absolute calorimetric calibration of low energy brachytherapy sources

    NASA Astrophysics Data System (ADS)

    Stump, Kurt E.

    In the past decade there has been a dramatic increase in the use of permanent radioactive source implants in the treatment of prostate cancer. A small radioactive source encapsulated in a titanium shell is used in this type of treatment. The radioisotopes used are generally 125I or 103Pd. Both of these isotopes have relatively short half-lives, 59.4 days and 16.99 days, respectively, and have low-energy emissions and a low dose rate. These factors make these sources well suited for this application, but the calibration of these sources poses significant metrological challenges. The current standard calibration technique involves the measurement of ionization in air to determine the source air-kerma strength. While this has proved to be an improvement over previous techniques, the method has been shown to be metrologically impure and may not be the ideal means of calbrating these sources. Calorimetric methods have long been viewed to be the most fundamental means of determining source strength for a radiation source. This is because calorimetry provides a direct measurement of source energy. However, due to the low energy and low power of the sources described above, current calorimetric methods are inadequate. This thesis presents work oriented toward developing novel methods to provide direct and absolute measurements of source power for low-energy low dose rate brachytherapy sources. The method is the first use of an actively temperature-controlled radiation absorber using the electrical substitution method to determine total contained source power of these sources. The instrument described operates at cryogenic temperatures. The method employed provides a direct measurement of source power. The work presented here is focused upon building a metrological foundation upon which to establish power-based calibrations of clinical-strength sources. To that end instrument performance has been assessed for these source strengths. The intent is to establish the limits of

  12. Past, present and future low energy antiproton facilities at CERN

    NASA Astrophysics Data System (ADS)

    Bartmann, W.; Belochitskii, P.; Breuker, H.; Butin, F.; Carli, C.; Eriksson, T.; Maury, S.; Oelert, W.; Pasinelli, S.; Tranquille, G.

    2014-05-01

    Low energy antiprotons are available for physics experiments at CERN since the 1980s and have been used by a large variety of experiments. The Low Energy Antiproton Ring LEAR has been constructed as a complementary use of antiprotons available at that time for high energy physics and delivered beam to experiments mainly using slow extraction. After completion of LEAR exploitation, the Antiproton Decelerator (AD) was constructed (adaptation of the existing Antiproton Collector, AC) to allow for a simpler low energy antiproton scheme (only one accelerator operated with Antiprotons) with fast extraction well suited for trap experiments. The Extra Low ENergy Antiproton ring ELENA is a small synchrotron presently constructed to further decelerate antiprotons from the AD in a controlled manner, and to reduce emittances with the help of an electron cooler to improve the capture efficiencies of existing experiments and allow for additional ones.

  13. Radial Flux Distribution of Low-Energy Neutrons.

    ERIC Educational Resources Information Center

    Higinbotham, J.

    1979-01-01

    Describes an experiment designed to illustrate the basic principle involved in the process of moderation of fast neutrons by water, and the monitoring of the low-energy neutron flux using indium as a probe. (GA)

  14. Biological assessments for the low energy demonstration accelerator, 1996

    SciTech Connect

    Cross, S.

    1997-03-01

    This report discusses the biological impact to the area around the Los Alamos National Laboratory of the Low Energy Demonstration Accelerator. In particular the impact to the soils, water quality, vegetation, and wildlife are discussed.

  15. Beam lifetime and limitations during low-energy RHIC operation

    SciTech Connect

    Fedotov, A.V.; Bai, M.; Blaskiewicz, M.; Fischer, W.; Kayran, D.; Montag, C.; Satogata, T.; Tepikian, S.; Wang, G.

    2011-03-28

    The low-energy physics program at the Relativistic Heavy Ion Collider (RHIC), motivated by a search for the QCD phase transition critical point, requires operation at low energies. At these energies, large nonlinear magnetic field errors and large beam sizes produce low beam lifetimes. A variety of beam dynamics effects such as Intrabeam Scattering (IBS), space charge and beam-beam forces also contribute. All these effects are important to understand beam lifetime limitations in RHIC at low energies. During the low-energy RHIC physics run in May-June 2010 at beam {gamma} = 6.1 and {gamma} = 4.1, gold beam lifetimes were measured for various values of space-charge tune shifts, transverse acceptance limitation by collimators, synchrotron tunes and RF voltage. This paper summarizes our observations and initial findings.

  16. Screening for antibacterial and antifungal activities in some marine algae from the Fujian coast of China with three different solvents

    NASA Astrophysics Data System (ADS)

    Zheng, Yi; Chen, Yin-Shan; Lu, Hai-Sheng

    2001-12-01

    Three different solvents viz ethanol, acetone and methanol-toluene (3:1) were used to extract antibiotics from 23 species of marine algae belonging to the Chlorophyta, Phaeophyta and Rhodophyta. Their crude extracts were tested for antibacterial and antifungal activities. Among them, the ethanol extract showed the strongest activity against the bacteria and fungi tested. Four species of the Rhodophyta ( Laurencia okamurai, Dasya scoparia, Grateloupia filicina and plocamium telfairiae) showed a wide spectrum of antibacterial activity. Every solvent extract from the four species was active against all the bacteria tested. The test bacterium Pseudomonas solancearum and the fungus Penicilium citrinum were most sensitive to the extracts of marine algae. In general, the extracts of seaweeds inhibited bacteria more strongly than fungi and species of the Rhodophyta showed the greatest activity against the bacteria and fungi tested.

  17. Anti-biofilm activity of the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125.

    PubMed

    Papa, Rosanna; Parrilli, Ermenegilda; Sannino, Filomena; Barbato, Gaetano; Tutino, Maria Luisa; Artini, Marco; Selan, Laura

    2013-06-01

    Considering the increasing impact of bacterial biofilms on human health, industrial and food-processing activities, the interest in the development of new approaches for the prevention and treatment of adhesion and biofilm formation capabilities has increased. A viable approach should target adhesive properties without affecting bacterial vitality in order to avoid the rapid appearance of escape mutants. It is known that marine bacteria belonging to the genus Pseudoalteromonas produce compounds of biotechnological interest, including anti-biofilm molecules. Pseudoalteromonas haloplanktis TAC125 is the first Antarctic Gram-negative strain whose genome was sequenced. In this work the anti-biofilm activity of P. haloplanktis supernatant was examined on different staphylococci. Results obtained demonstrated that supernatant of P. haloplanktis, grown in static condition, inhibits biofilm of Staphylococcus epidermidis. In order to define the chemical nature of the biofilm-inhibiting compound, the supernatant was subject to various treatments. Data reported demonstrated that the biologically active component is sensible to treatment with sodium periodate suggesting its saccharidic nature. PMID:23411371

  18. Treatment of saline wastewaters from marine-products processing factories by activated sludge reactor.

    PubMed

    Khannous, L; Souissi, N; Ghorbel, B; Jarboui, R; Kallel, M; Nasri, M; Gharsallah, N

    2003-10-01

    An activated sludge reactor, operated at room temperature (20-30 degrees C) was used to treat saline wastewaters generated by marine-products industries. The system was operated continuously and the influence of the organic loading rates (OLRs), varying from 250 to 1000 mg COD l(-1) day(-1), on chemical oxygen demand (COD) removal was investigated. The system, inoculated with NaCl-acclimated culture, removed up to 98% and 88% of the influent COD concentrations at OLRs of 250 and 1000 mg COD L(-1) day(-1), respectively. Since the organic pollution is essentially composed of proteins, microorganisms, which produced proteolytic enzymes, were isolated from the activated sludge culture. One bacterium with the highest protease activity, identified as Bacillus cereus, was chosen for protease production in fishery wastewaters of different concentrations containing combined heads and viscera powder. Protease synthesis was strongly enhanced when cells were cultivated in two times diluted fishery wastewaters. The enhancement of protease synthesis could have been due to the presence in effluent of organic matters or salts, which stimulated the growth of the strain and protease production.

  19. (129)I record of nuclear activities in marine sediment core from Jiaozhou Bay in China.

    PubMed

    Fan, Yukun; Hou, Xiaolin; Zhou, Weijian; Liu, Guangshan

    2016-04-01

    Iodine-129 has been used as a powerful tool for environmental tracing of human nuclear activities. In this work, a sediment core collected from Jiaozhou Bay, the east coast of China, in 2002 was analyzed for (129)I to investigate the influence of human nuclear activities in this region. Significantly enhanced (129)I level was observed in upper 70 cm of the sediment core, with peak values in the layer corresponding to 1957, 1964, 1974, 1986, and after 1990. The sources of (129)I and corresponding transport processes in this region are discussed, including nuclear weapons testing at the Pacific Proving Grounds, global fallout from a large numbers of nuclear weapon tests in 1963, the climax of Chinese nuclear weapons testing in the early 1970s, the Chernobyl accident in 1986, and long-distance dispersion of European reprocessing derived (129)I. The very well (129)I records of different human nuclear activities in the sediment core illustrate the potential application of (129)I in constraining ages and sedimentation rates of the recent sediment. The releases of (129)I from the European nuclear fuel reprocessing plants at La Hague (France) and Sellafield (UK) were found to dominate the inventory of (129)I in the Chinese sediments after 1990, not only the directly atmospheric releases of these reprocessing plants, but also re-emission of marine discharged (129)I of these reprocessing plants in the highly contaminated European seas. PMID:26821329

  20. A marine algicidal Thalassospira and its active substance against the harmful algal bloom species Karenia mikimotoi.

    PubMed

    Lu, Xiuhua; Zhou, Bin; Xu, Lili; Liu, Lin; Wang, Gangyuan; Liu, Xiaodong; Tang, Xuexi

    2016-06-01

    The aim of the present study was to obtain a marine bacterium active against Karenia mikimotoi from the East China Sea and to characterize its extracellular algicidal substances. Using preparative high-performance liquid chromatography (prep-HPLC) and electrospray ionization/quadrupole-time of flight mass spectrometer coupled with a high-performance liquid chromatography (LC/MS-Q-TOF) system, we purified the alga-lysing substance produced by strain ZR-2 and determined its molecular structure. Based on morphology and l6S ribosomal DNA (rDNA) sequence analysis, the ZR-2 strain was highly homologous to Thalassospira species. Algicidal activity against K. mikimotoi was detected in the cell-free filtrate but not in bacterial cells. The alga-lysing substance produced by ZR-2 was ethanol-soluble and thermostable, with a retention time of 6.3 min and a measured elemental composition of C7H5O2 ([M-H](-) ion at m/z 121.0295). The alga-lysing substance produced by ZR-2 was determined to be benzoic acid. Compared with the negative control, both purified ZR-2 bacteria-free filtrate and standard benzoic acid promoted K. mikimotoi cell disruption and induced K. mikimotoi cell content leakage. Our study is the first to report benzoic acid activity against K. mikimotoi as well as production of benzoic acid by a Thalassospira species. PMID:26846742

  1. Antitumor and immune regulation activities of the extracts of some Chinese marine invertebrates

    NASA Astrophysics Data System (ADS)

    Zhang, Lixin; Fan, Xiao; Han, Lijun

    2005-03-01

    Extracts of 21 marine invertebrates belonging to Coelenterata, Mollusca, Annelida, Bryozoa, Echiura, Arthropoda, Echinodermata and Urochordata were screened for the studies on their antitumor and immune regulation activities. Antitumor activity was determined by MTT method and immune regulation activity was studied using T- and B-lymphocytes in mice spleen in vitro. It was found that the n-butanol part of Asterina pectinifera, the acetic ether part of Tubuaria marina, 95% ethanol extract of Acanthochiton rubrolineatus have a high inhibition rate of 96.7%, 63.9% and 50.5% respectively on tumor cell line HL-60 at the concentration of 0.063 mg/ml. The inhibition rate of the acetic ether part of Tubuaria marina on the tumor cell line A-549 is 65.4% at concentration of 0.063 mg/mL. The 95% ethanol extract of Meretrix meretrix has so outstanding promoting effect on T-lymphocytes that their multiplication increases 25% when the sample concentration is only 1 μg/ml. On B-lymphocytes, the 95% extract of Rapana venosa, at concentration of 100 μg/ml, has a promotion percentage of 60%. On the other hand, under the condition of no cytotoxic effect, the 95% ethanol extracts of Acanthochiton rubrolineatus and Cellana toreum can reach 92% inhibition rate on T lymphocyte at concentration of 100 μg/ml, while the inhibition rate on B lymphocyte of the 95% extract of Acanthochiton rubrolineatus reaches 92% at the same concentration.

  2. Treatment of saline wastewaters from marine-products processing factories by activated sludge reactor.

    PubMed

    Khannous, L; Souissi, N; Ghorbel, B; Jarboui, R; Kallel, M; Nasri, M; Gharsallah, N

    2003-10-01

    An activated sludge reactor, operated at room temperature (20-30 degrees C) was used to treat saline wastewaters generated by marine-products industries. The system was operated continuously and the influence of the organic loading rates (OLRs), varying from 250 to 1000 mg COD l(-1) day(-1), on chemical oxygen demand (COD) removal was investigated. The system, inoculated with NaCl-acclimated culture, removed up to 98% and 88% of the influent COD concentrations at OLRs of 250 and 1000 mg COD L(-1) day(-1), respectively. Since the organic pollution is essentially composed of proteins, microorganisms, which produced proteolytic enzymes, were isolated from the activated sludge culture. One bacterium with the highest protease activity, identified as Bacillus cereus, was chosen for protease production in fishery wastewaters of different concentrations containing combined heads and viscera powder. Protease synthesis was strongly enhanced when cells were cultivated in two times diluted fishery wastewaters. The enhancement of protease synthesis could have been due to the presence in effluent of organic matters or salts, which stimulated the growth of the strain and protease production. PMID:14669806

  3. A marine algicidal Thalassospira and its active substance against the harmful algal bloom species Karenia mikimotoi.

    PubMed

    Lu, Xiuhua; Zhou, Bin; Xu, Lili; Liu, Lin; Wang, Gangyuan; Liu, Xiaodong; Tang, Xuexi

    2016-06-01

    The aim of the present study was to obtain a marine bacterium active against Karenia mikimotoi from the East China Sea and to characterize its extracellular algicidal substances. Using preparative high-performance liquid chromatography (prep-HPLC) and electrospray ionization/quadrupole-time of flight mass spectrometer coupled with a high-performance liquid chromatography (LC/MS-Q-TOF) system, we purified the alga-lysing substance produced by strain ZR-2 and determined its molecular structure. Based on morphology and l6S ribosomal DNA (rDNA) sequence analysis, the ZR-2 strain was highly homologous to Thalassospira species. Algicidal activity against K. mikimotoi was detected in the cell-free filtrate but not in bacterial cells. The alga-lysing substance produced by ZR-2 was ethanol-soluble and thermostable, with a retention time of 6.3 min and a measured elemental composition of C7H5O2 ([M-H](-) ion at m/z 121.0295). The alga-lysing substance produced by ZR-2 was determined to be benzoic acid. Compared with the negative control, both purified ZR-2 bacteria-free filtrate and standard benzoic acid promoted K. mikimotoi cell disruption and induced K. mikimotoi cell content leakage. Our study is the first to report benzoic acid activity against K. mikimotoi as well as production of benzoic acid by a Thalassospira species.

  4. (129)I record of nuclear activities in marine sediment core from Jiaozhou Bay in China.

    PubMed

    Fan, Yukun; Hou, Xiaolin; Zhou, Weijian; Liu, Guangshan

    2016-04-01

    Iodine-129 has been used as a powerful tool for environmental tracing of human nuclear activities. In this work, a sediment core collected from Jiaozhou Bay, the east coast of China, in 2002 was analyzed for (129)I to investigate the influence of human nuclear activities in this region. Significantly enhanced (129)I level was observed in upper 70 cm of the sediment core, with peak values in the layer corresponding to 1957, 1964, 1974, 1986, and after 1990. The sources of (129)I and corresponding transport processes in this region are discussed, including nuclear weapons testing at the Pacific Proving Grounds, global fallout from a large numbers of nuclear weapon tests in 1963, the climax of Chinese nuclear weapons testing in the early 1970s, the Chernobyl accident in 1986, and long-distance dispersion of European reprocessing derived (129)I. The very well (129)I records of different human nuclear activities in the sediment core illustrate the potential application of (129)I in constraining ages and sedimentation rates of the recent sediment. The releases of (129)I from the European nuclear fuel reprocessing plants at La Hague (France) and Sellafield (UK) were found to dominate the inventory of (129)I in the Chinese sediments after 1990, not only the directly atmospheric releases of these reprocessing plants, but also re-emission of marine discharged (129)I of these reprocessing plants in the highly contaminated European seas.

  5. Pre-Trip Expectations and Post-Trip Satisfaction with Marine Tour Interpretation in Hawaii: Applying the Norm Activation Model

    ERIC Educational Resources Information Center

    Littlejohn, Kerrie; Needham, Mark D.; Szuster, Brian W.; Jordan, Evan J.

    2016-01-01

    This article examines environmental education by focusing on recreationist expectations for interpretation on marine tours, satisfaction with this interpretation and whether expectations were met, and how these perceptions correlate with components of the norm activation model. Recreationists surveyed before and after tours to Molokini, Hawaii (n…

  6. Exploring Marine Ecosystems with Elementary School Portuguese Children: Inquiry-Based Project Activities Focused on "Real-Life" Contexts

    ERIC Educational Resources Information Center

    Guilherme, Elsa; Faria, Cláudia; Boaventura, Diana

    2016-01-01

    The purpose of the study was to investigate how young students engage in an inquiry-based project driven by real-life contexts. Elementary school children were engaged in a small inquiry project centred on marine biodiversity and species adaptations. All activities included the exploration of an out-of-school setting as a learning context. A total…

  7. 30 CFR 585.801 - How must I conduct my approved activities to protect marine mammals, threatened and endangered...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... protect marine mammals, threatened and endangered species, and designated critical habitat? 585.801... endangered species, and designated critical habitat? (a) You must not conduct any activity under your lease or grant that may affect threatened or endangered species or that may affect designated...

  8. 30 CFR 285.801 - How must I conduct my approved activities to protect marine mammals, threatened and endangered...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... protect marine mammals, threatened and endangered species, and designated critical habitat? 285.801... endangered species, and designated critical habitat? (a) You must not conduct any activity under your lease or grant that may affect threatened or endangered species or that may affect designated...

  9. 30 CFR 585.801 - How must I conduct my approved activities to protect marine mammals, threatened and endangered...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... protect marine mammals, threatened and endangered species, and designated critical habitat? 585.801... endangered species, and designated critical habitat? (a) You must not conduct any activity under your lease or grant that may affect threatened or endangered species or that may affect designated...

  10. 30 CFR 585.801 - How must I conduct my approved activities to protect marine mammals, threatened and endangered...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... protect marine mammals, threatened and endangered species, and designated critical habitat? 585.801... endangered species, and designated critical habitat? (a) You must not conduct any activity under your lease or grant that may affect threatened or endangered species or that may affect designated...

  11. 30 CFR 285.801 - How must I conduct my approved activities to protect marine mammals, threatened and endangered...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... protect marine mammals, threatened and endangered species, and designated critical habitat? 285.801... mammals, threatened and endangered species, and designated critical habitat? (a) You must not conduct any activity under your lease or grant that may affect threatened or endangered species or that may...

  12. 78 FR 49729 - Takes of Marine Mammals Incidental to Specified Activities; U.S. Air Force Launches, Aircraft and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-15

    ... Related to Launch Vehicles From Vandenberg Air Force Base (VAFB), California AGENCY: National Marine... incidental to launching space launch vehicles, intercontinental ballistic and small missiles, aircraft and helicopter operations, and harbor activities related to the Delta IV/Evolved Expendable Launch Vehicle...

  13. S.E.A. Lab. Science Experiments and Activities. Marine Science for High School Students in Chemistry, Biology and Physics.

    ERIC Educational Resources Information Center

    Hart, Kathy, Ed.

    A series of science experiments and activities designed for secondary school students taking biology, chemistry, physics, physical science or marine science courses are outlined. Each of the three major sections--chemistry, biology, and physics--addresses concepts that are generally covered in those courses but incorporates aspects of marine…

  14. 75 FR 5055 - Taking and Importing Marine Mammals; U.S. Navy's Atlantic Fleet Active Sonar Training (AFAST)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... to AFAST training, maintenance, and RDT&E became effective on January 22, 2009 (74 FR 4843, January... conducted within the AFAST Study Area under regulations issued on January 22, 2009 (74 FR 4843, January 27.... Navy's Atlantic Fleet Active Sonar Training (AFAST) AGENCY: National Marine Fisheries Service...

  15. 78 FR 7049 - Takes of Marine Mammals Incidental to Specified Activities; U.S. Navy Training and Testing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-31

    ... Gulf of Mexico (GOMEX) EIS/OEIS (U.S. Department of the Navy, 2011). These documents, among others, and... effects analysis presented in the Navy's LOA application differ from the quantified results presented in... Part 218 Takes of Marine Mammals Incidental to Specified Activities; U.S. Navy Training and...

  16. 77 FR 50473 - Taking of Marine Mammals Incidental to Specified Activities; Construction of the East Span of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... February 6, 2012 (76 FR 7156, February 9, 2011). No in-water construction activity was conducted during the... provided in the January 26, 2006 (71 FR 4352), Federal Register notice. Potential Effects on Marine Mammals... SPL) in the world's ocean from pre-industrial periods, and most of these increases are from...

  17. Surface Passivation and Junction Formation Using Low Energy Hydrogen Implants

    NASA Technical Reports Server (NTRS)

    Fonash, S. J.

    1985-01-01

    New applications for high current, low energy hydrogen ion implants on single crystal and polycrystal silicon grain boundaries are discussed. The effects of low energy hydrogen ion beams on crystalline Si surfaces are considered. The effect of these beams on bulk defects in crystalline Si is addressed. Specific applications of H+ implants to crystalline Si processing are discussed. In all of the situations reported on, the hydrogen beams were produced using a high current Kaufman ion source.

  18. Strong flux of low-energy neutrons produced by thunderstorms.

    PubMed

    Gurevich, A V; Antonova, V P; Chubenko, A P; Karashtin, A N; Mitko, G G; Ptitsyn, M O; Ryabov, V A; Shepetov, A L; Shlyugaev, Yu V; Vildanova, L I; Zybin, K P

    2012-03-23

    We report here for the first time about the registration of an extraordinary high flux of low-energy neutrons generated during thunderstorms. The measured neutron count rate enhancements are directly connected with thunderstorm discharges. The low-energy neutron flux value obtained in our work is a challenge for the photonuclear channel of neutron generation in thunderstorm: the estimated value of the needed high-energy γ-ray flux is about 3 orders of magnitude higher than that one observed.

  19. Monitoring Spawning Activity in a Southern California Marine Protected Area Using Molecular Identification of Fish Eggs

    PubMed Central

    Harada, Alice E.; Lindgren, Elise A.; Hermsmeier, Maiko C.; Rogowski, Peter A.; Terrill, Eric; Burton, Ronald S.

    2015-01-01

    In order to protect the diverse ecosystems of coastal California, a series of marine protected areas (MPAs) have been established. The ability of these MPAs to preserve and potentially enhance marine resources can only be assessed if these habitats are monitored through time. This study establishes a baseline for monitoring the spawning activity of fish in the MPAs adjacent to Scripps Institution of Oceanography (La Jolla, CA, USA) by sampling fish eggs from the plankton. Using vertical plankton net tows, 266 collections were made from the Scripps Pier between 23 August 2012 and 28 August 2014; a total of 21,269 eggs were obtained. Eggs were identified using DNA barcoding: the COI or 16S rRNA gene was amplified from individual eggs and sequenced. All eggs that were successfully sequenced could be identified from a database of molecular barcodes of California fish species, resulting in species-level identification of 13,249 eggs. Additionally, a surface transport model of coastal circulation driven by current maps from high frequency radar was used to construct probability maps that estimate spawning locations that gave rise to the collected eggs. These maps indicated that currents usually come from the north but water parcels tend to be retained within the MPA; eggs sampled at the Scripps Pier have a high probability of having been spawned within the MPA. The surface transport model also suggests that although larvae have a high probability of being retained within the MPA, there is also significant spillover into nearby areas outside the MPA. This study provides an important baseline for addressing the extent to which spawning patterns of coastal California species may be affected by future changes in the ocean environment. PMID:26308928

  20. Monitoring Spawning Activity in a Southern California Marine Protected Area Using Molecular Identification of Fish Eggs.

    PubMed

    Harada, Alice E; Lindgren, Elise A; Hermsmeier, Maiko C; Rogowski, Peter A; Terrill, Eric; Burton, Ronald S

    2015-01-01

    In order to protect the diverse ecosystems of coastal California, a series of marine protected areas (MPAs) have been established. The ability of these MPAs to preserve and potentially enhance marine resources can only be assessed if these habitats are monitored through time. This study establishes a baseline for monitoring the spawning activity of fish in the MPAs adjacent to Scripps Institution of Oceanography (La Jolla, CA, USA) by sampling fish eggs from the plankton. Using vertical plankton net tows, 266 collections were made from the Scripps Pier between 23 August 2012 and 28 August 2014; a total of 21,269 eggs were obtained. Eggs were identified using DNA barcoding: the COI or 16S rRNA gene was amplified from individual eggs and sequenced. All eggs that were successfully sequenced could be identified from a database of molecular barcodes of California fish species, resulting in species-level identification of 13,249 eggs. Additionally, a surface transport model of coastal circulation driven by current maps from high frequency radar was used to construct probability maps that estimate spawning locations that gave rise to the collected eggs. These maps indicated that currents usually come from the north but water parcels tend to be retained within the MPA; eggs sampled at the Scripps Pier have a high probability of having been spawned within the MPA. The surface transport model also suggests that although larvae have a high probability of being retained within the MPA, there is also significant spillover into nearby areas outside the MPA. This study provides an important baseline for addressing the extent to which spawning patterns of coastal California species may be affected by future changes in the ocean environment. PMID:26308928

  1. Kalkipyrone B, a marine cyanobacterial γ-pyrone possessing cytotoxic and anti-fungal activities.

    PubMed

    Bertin, Matthew J; Demirkiran, Ozlem; Navarro, Gabriel; Moss, Nathan A; Lee, John; Goldgof, Gregory M; Vigil, Edgar; Winzeler, Elizabeth A; Valeriote, Fred A; Gerwick, William H

    2016-02-01

    Bioassay-guided fractionation of two marine cyanobacterial extracts using the H-460 human lung cancer cell line and the OVC-5 human ovarian cancer cell line led to the isolation of three related α-methoxy-β, β'-dimethyl-γ-pyrones each containing a modified alkyl chain, one of which was identified as the previously reported kalkipyrone and designated kalkipyrone A. The second compound was an analog designated kalkipyrone B. The third was identified as the recently reported yoshinone A, also isolated from a marine cyanobacterium. Kalkipyrone A and B were obtained from a field-collection of the cyanobacterium Leptolyngbya sp. from Fagasa Bay, American Samoa, while yoshinone A was isolated from a field-collection of cyanobacteria (cf. Schizothrix sp.) from Panama. One-dimensional and two-dimensional NMR experiments were used to determine the overall structures and relative configurations of the kalkipyrones, and the absolute configuration of kalkipyrone B was determined by (1)H NMR analysis of diastereomeric Mosher's esters. Kalkipyrone A showed good cytotoxicity to H-460 human lung cancer cells (EC50=0.9μM), while kalkipyrone B and yoshinone A were less active (EC50=9.0μM and >10μM, respectively). Both kalkipyrone A and B showed moderate toxicity to Saccharomyces cerevisiae ABC16-Monster strain (IC50=14.6 and 13.4μM, respectively), whereas yoshinone A was of low toxicity to this yeast strain (IC50=63.8μM). PMID:26632528

  2. Anti-Biofilm Activities from Marine Cold Adapted Bacteria Against Staphylococci and Pseudomonas aeruginosa

    PubMed Central

    Papa, Rosanna; Selan, Laura; Parrilli, Ermenegilda; Tilotta, Marco; Sannino, Filomena; Feller, Georges; Tutino, Maria L.; Artini, Marco

    2015-01-01

    Microbial biofilms have great negative impacts on the world’s economy and pose serious problems to industry, public health and medicine. The interest in the development of new approaches for the prevention and treatment of bacterial adhesion and biofilm formation has increased. Since, bacterial pathogens living in biofilm induce persistent chronic infections due to the resistance to antibiotics and host immune system. A viable approach should target adhesive properties without affecting bacterial vitality in order to avoid the appearance of resistant mutants. Many bacteria secrete anti-biofilm molecules that function in regulating biofilm architecture or mediating the release of cells from it during the dispersal stage of biofilm life cycle. Cold-adapted marine bacteria represent an untapped reservoir of biodiversity able to synthesize a broad range of bioactive compounds, including anti-biofilm molecules. The anti-biofilm activity of cell-free supernatants derived from sessile and planktonic cultures of cold-adapted bacteria belonging to Pseudoalteromonas, Psychrobacter, and Psychromonas species were tested against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa strains. Reported results demonstrate that we have selected supernatants, from cold-adapted marine bacteria, containing non-biocidal agents able to destabilize biofilm matrix of all tested pathogens without killing cells. A preliminary physico-chemical characterization of supernatants was also performed, and these analyses highlighted the presence of molecules of different nature that act by inhibiting biofilm formation. Some of them are also able to impair the initial attachment of the bacterial cells to the surface, thus likely containing molecules acting as anti-biofilm surfactant molecules. The described ability of cold-adapted bacteria to produce effective anti-biofilm molecules paves the way to further characterization of the most promising molecules and to test their

  3. Anti-Biofilm Activities from Marine Cold Adapted Bacteria Against Staphylococci and Pseudomonas aeruginosa.

    PubMed

    Papa, Rosanna; Selan, Laura; Parrilli, Ermenegilda; Tilotta, Marco; Sannino, Filomena; Feller, Georges; Tutino, Maria L; Artini, Marco

    2015-01-01

    Microbial biofilms have great negative impacts on the world's economy and pose serious problems to industry, public health and medicine. The interest in the development of new approaches for the prevention and treatment of bacterial adhesion and biofilm formation has increased. Since, bacterial pathogens living in biofilm induce persistent chronic infections due to the resistance to antibiotics and host immune system. A viable approach should target adhesive properties without affecting bacterial vitality in order to avoid the appearance of resistant mutants. Many bacteria secrete anti-biofilm molecules that function in regulating biofilm architecture or mediating the release of cells from it during the dispersal stage of biofilm life cycle. Cold-adapted marine bacteria represent an untapped reservoir of biodiversity able to synthesize a broad range of bioactive compounds, including anti-biofilm molecules. The anti-biofilm activity of cell-free supernatants derived from sessile and planktonic cultures of cold-adapted bacteria belonging to Pseudoalteromonas, Psychrobacter, and Psychromonas species were tested against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa strains. Reported results demonstrate that we have selected supernatants, from cold-adapted marine bacteria, containing non-biocidal agents able to destabilize biofilm matrix of all tested pathogens without killing cells. A preliminary physico-chemical characterization of supernatants was also performed, and these analyses highlighted the presence of molecules of different nature that act by inhibiting biofilm formation. Some of them are also able to impair the initial attachment of the bacterial cells to the surface, thus likely containing molecules acting as anti-biofilm surfactant molecules. The described ability of cold-adapted bacteria to produce effective anti-biofilm molecules paves the way to further characterization of the most promising molecules and to test their

  4. 77 FR 38587 - Takes of Marine Mammals Incidental to Specified Activities; Harbor Activities Related to the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-28

    ... 2002 to 2011, with the last Authorization expiring on June 6, 2012 (76 FR 33721, June 9, 2011). United... associated with these activities. Pinnipeds sometimes show startle reactions when exposed to sudden brief... core off-loading procedures, may elicit such a reaction. In addition, the movements of cranes...

  5. Quantification of extracellular carbonic anhydrase activity in two marine diatoms and investigation of its role.

    PubMed

    Hopkinson, Brian M; Meile, Christof; Shen, Chen

    2013-06-01

    Many microalgae induce an extracellular carbonic anhydrase (eCA), associated with the cell surface, at low carbon dioxide (CO2) concentrations. This enzyme is thought to aid inorganic carbon uptake by generating CO2 at the cell surface, but alternative roles have been proposed. We developed a new approach to quantify eCA activity in which a reaction-diffusion model is fit to data on (18)O removal from inorganic carbon. In contrast to previous methods, eCA activity is treated as a surface process, allowing the effects of eCA on cell boundary-layer chemistry to be assessed. Using this approach, we measured eCA activity in two marine diatoms (Thalassiosira pseudonana and Thalassiosira weissflogii), characterized the kinetics of this enzyme, and studied its regulation as a function of culture pH and CO2 concentration. In support of a role for eCA in CO2 supply, eCA activity specifically responded to low CO2 rather than to changes in pH or HCO3(-), and the rates of eCA activity are nearly optimal for maintaining cell surface CO2 concentrations near those in the bulk solution. Although the CO2 gradients abolished by eCA are small (less than 0.5 μm concentration difference between bulk and cell surface), CO2 uptake in these diatoms is a passive process driven by small concentration gradients. Analysis of the effects of short-term and long-term eCA inhibition on photosynthesis and growth indicates that eCA provides a small energetic benefit by reducing the surface-to-bulk CO2 gradient. Alternative roles for eCA in CO2 recovery as HCO3(-) and surface pH regulation were investigated, but eCA was found to have minimal effects on these processes.

  6. 15 CFR 922.73 - Additional prohibited or otherwise regulated activities-marine reserves and marine conservation...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... prohibited by 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and... 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and thus... (ii) Commercial and recreational fishing for lobster. (2) Possessing fishing gear on board a...

  7. 15 CFR 922.73 - Additional prohibited or otherwise regulated activities-marine reserves and marine conservation...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... prohibited by 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and... 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and thus... (ii) Commercial and recreational fishing for lobster. (2) Possessing fishing gear on board a...

  8. 15 CFR 922.73 - Additional prohibited or otherwise regulated activities-marine reserves and marine conservation...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... prohibited by 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and... 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and thus... (ii) Commercial and recreational fishing for lobster. (2) Possessing fishing gear on board a...

  9. 15 CFR 922.73 - Additional prohibited or otherwise regulated activities-marine reserves and marine conservation...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... prohibited by 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and... 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and thus... (ii) Commercial and recreational fishing for lobster. (2) Possessing fishing gear on board a...

  10. 15 CFR 922.73 - Additional prohibited or otherwise regulated activities-marine reserves and marine conservation...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... prohibited by 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and... 50 CFR part 660 (Fisheries off West Coast States), the following activities are prohibited and thus... (ii) Commercial and recreational fishing for lobster. (2) Possessing fishing gear on board a...

  11. Phosphate oxygen isotope ratio proxy for specific microbial activity in marine sediments (Peru Margin)

    NASA Astrophysics Data System (ADS)

    Liang, Y.; Blake, R. E.

    2005-12-01

    Oxygen (O) isotope ratios of biogenic apatites have been widely used as paleotemperature and environmental geochemical proxies. With improved knowledge of the phosphate O isotope effects of different P cycling pathways, the δ18O value of inorganic phosphate (δ18OP) has been proposed as a useful proxy and tracer of biological reactions and P cycling in natural environments[1,2,3,4]. Being the only way of removing P from oceanic water, sedimentary P burial is one of the most important processes during biogeochemical cycling of P. The high concentrations of organic matter and pronounced microbial activity at ODP Site 1230 along the Peru Margin result in unusually high interstitial water phosphate concentrations, which provides a unique opportunity to use δ18OP to investigate inorganic phosphate (Pi) regeneration and P cycling pathways in marine sediments. The isotopic measurements of both dissolved inorganic phosphate (DIP) and bulk sediment Pi show that DIP δ18OP values are affected by three different processes, which are all induced by specific microbial activities present in the sediments. In sediments at ~ 65 to 120 mbsf, porewater DIP is derived from dissolved organophosphorus compounds (DOP) through enzymatic degradation pathways, evidenced by both DIP δ18OP values and interstitial water chemistry. Measured porewater DIP δ18OP values also suggest that 4 to 8% of interstitial water DIP reflects regeneration of Pi from Porg by microbially-synthesized enzymes. Throughout the sediment column and especially at ~ 120 to 150 mbsf, DIP is released from the sediments by microbially-induced reductive dissolution of Fe-oxides, which contributes to the overall high DIP concentrations at Site 1230. The third and dominant process controlling measured DIP δ18OP values is microbial turnover of regenerated Pi. The presence of high microbial activities in organic-rich Site 1230 sediments promotes the remobilization of P and affects marine P cycling by potentially enhancing

  12. Seeking to Improve Low Energy Neutral Atom Detection in Space

    NASA Technical Reports Server (NTRS)

    Shappirio, M.; Coplan, M.; Chornay, D.; Collier, M.; Herrero, F.; Ogilvie, K.; Williams, E.

    2007-01-01

    The detection of energetic neutral atoms allows for the remote examination of the interactions between plasmas and neutral populations in space. Before these neutral atoms can be measured, they must first be converted to ions. For the low energy end of this spectrum, interaction with a conversion surface is often the most efficient method to convert neutrals into ions. It is generally thought that the most efficient surfaces are low work functions materials. However, by their very nature, these surfaces are highly reactive and unstable, and therefore are not suitable for space missions where conditions cannot be controlled as they are in a laboratory. We therefore are looking to optimize a stable surface for conversion efficiency. Conversion efficiency can be increased either by changing the incident angle of the neutral particles to be grazing incidence and using stable surfaces with high conversion efficiencies. We have examined how to increase the angle of incidence from -80 degrees to -89 degrees, while maintaining or improving the total active conversion surface area without increasing the overall volume of the instrument. We are developing a method to micro-machine silicon, which will reduce the volume to surface area ratio by a factor of 60. We have also examined the material properties that affect the conversion efficiency of the surface for stable surfaces. Some of the parameters we have examined are work function, smoothness, and bond structure. We find that for stable surfaces, the most important property is the smoothness of the surface.

  13. Biophysics behavior of acupuncture points irradiated with low energy lasers.

    PubMed

    Moldovan, C

    2007-01-01

    This work describes the Low Energy Laser (LEL) coherent light interaction with the skin cover on acupuncture loci for the purpose of detecting and measuring the spatial and temporal alteration of the thermal, electric and optical properties of the LI4 (HEGU) acupoint, irradiated with a 685 nm, 30 mW, III.B Laser. Novel electrostatic imaging technique, an original Acupuncture 3-D Thermal and Electric Mapping Technique and an original Method for Laser-Skin Reflectance, were used in the study. The results indicate that the visible laser light, with low frequency and low power, specifically modify the 3-D pattern of the temperature, electric potential and electric impedance outline of an acupuncture point, meanwhile with a significant decrease of the laser reflectance index, all measured on a 27 apparently healthy subject lot (48 years mean age, 54% male), when comparing with a non-active, non-acupunctural skin area, placed on the volar side of the same hand. The biophysical method presented, combines in a complex way and reproducible the electro stasis exploration (bioelectric homeostasis), with cutaneous thermodynamic exploration and photo-optical exploration of the derma and provides information that can be appreciated in dynamics and compared depending on the exploration target.

  14. Anti-fish nodaviral activity of furan-2-yl acetate extracted from marine Streptomyces spp.

    PubMed

    Suthindhiran, K; Sarath Babu, V; Kannabiran, K; Ishaq Ahmed, V P; Sahul Hameed, A S

    2011-04-01

    The antiviral activity of furan-2-yl acetate (C₆H₆O₃) extracted from Streptomyces VITSDK1 spp. was studied in cultured Sahul Indian Grouper Eye (SIGE) cells infected with fish nodavirus (FNV). The nodavirus infection in the SIGE cells was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and the antiviral activity of furan-2-yl acetate was assessed by cytopathic effect, as well as reduction in nodaviral titre (TCID₅₀ mL⁻¹, where TCID₅₀) is the 50% tissue culture infective dose) in the cultured SIGE cells under in vitro conditions. Furan-2-yl acetate (20 µg mL⁻¹) effectively inhibited the replication of the FNV-infected SIGE cell lines and the viral titre was reduced from 4.3 to 2.45 log TCID₅₀ mL⁻¹ on treatments. Furan-2-yl acetate (20 µg mL⁻¹)- treated SIGE cell survival was found to be 90%, as determined by methyl thiazol tetrazolium assay. The results of an immunofluorescent assay revealed a strong association between the viral capsid protein inhibition and a decline in viral replication. The results suggest that furan-2-yl acetate suppressed FNV replication in cultured fish cells, providing a potential approach for the control of nodaviral diseases in marine fishes. PMID:21462077

  15. Effect of copper on growth and enzyme activities of marine diatom, Odontella mobiliensis.

    PubMed

    Manimaran, K; Karthikeyan, P; Ashokkumar, S; Ashok Prabu, V; Sampathkumar, P

    2012-01-01

    The 72-h IC(50), 7-d no observable effect concentration (NOEC), low observable effect concentration (LOEC), Chronic values were derived for copper on the growth of marine diatom, Odontella mobiliensis. The effect of copper was also studied on cell morphology, size, nitrate reductase and antioxidant enzymes (Catalase, Superoxide dismutase and peroxidase). The 72-h IC(50) of 298.4 ± 28.3, NOEC of 15.6, LOEC of 29.6 and chronic value of 21.5 μg Cu L(-1) were found in the present study. The chlorophyll a was significantly decreased with increasing concentrations of copper. The length of the cell (apical axis) was extended from 30.14 ± 5.98 μm at control to 71.4 ± 6.29 μm at 574 μg Cu L(-1), the spines were absent at 574 μg L(-1) and the cell structure was entirely damaged at 926 μg Cu L(-1). The antioxidant enzymes viz. Catalase, Peroxidase activities and Melondialdehyde were increased whereas the Nitrate reductase and activity was reduced at 21.5 μg Cu L(-1) during 7 days exposure. PMID:22016104

  16. The influence of marine microbial activities on aerosol production: A laboratory mesocosm study

    NASA Astrophysics Data System (ADS)

    Alpert, Peter A.; Kilthau, Wendy P.; Bothe, Dylan W.; Radway, JoAnn C.; Aller, Josephine Y.; Knopf, Daniel A.

    2015-09-01

    The oceans cover most of the Earth's surface, contain nearly half the total global primary biomass productivity, and are a major source of atmospheric aerosol particles. Here we experimentally investigate links between biological activity in seawater and sea spray aerosol (SSA) flux, a relationship of potential significance for organic aerosol loading and cloud formation over the oceans and thus for climate globally. Bubbles were generated in laboratory mesocosm experiments either by recirculating impinging water jets or glass frits. Experiments were conducted with Atlantic Ocean seawater collected off the eastern end of Long Island, NY, and with artificial seawater containing cultures of bacteria and phytoplankton Thalassiosira pseudonana, Emiliania huxleyi, and Nannochloris atomus. Changes in SSA size distributions occurred during all phases of bacterial and phytoplankton growth, as characterized by cell concentrations, dissolved organic carbon, total particulate carbon, and transparent exopolymer particles (gel-forming polysaccharides representing a major component of biogenic exudate material). Over a 2 week growth period, SSA particle concentrations increased by a factor of less than 2 when only bacteria were present and by a factor of about 3 when bacteria and phytoplankton were present. Production of jet-generated SSA particles of diameter less than 200 nm increased with time, while production of all particle diameters increased with time when frits were used. The implications of a marine biological activity dependent SSA flux are discussed.

  17. 76 FR 71940 - Takes of Marine Mammals Incidental to Specified Activities; Physical Oceanographic Studies in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-21

    ...NMFS has received an application from the United States Navy (Navy) for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment, incidental to conducting physical oceanographic studies in the southwest Indian Ocean, January through February, 2012. Pursuant to the Marine Mammal Protection Act (MMPA), NMFS is requesting comments on its proposal to issue an IHA to the......

  18. 77 FR 38832 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Marine...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-29

    ... notice published in the Federal Register on April 26, 2012 (77 FR 24990). Interested parties are...; Marine Terminals and Longshoring Standards ACTION: Notice. SUMMARY: On June 29, 2012, the Department of... collection request (ICR) titled, ``Marine Terminals and Longshoring Standards'' to the Office of...

  19. 76 FR 79157 - Takes of Marine Mammals Incidental to Specified Activities; St. George Reef Light Station...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-21

    ...), for an Incidental Harassment Authorization (IHA) to take marine mammals, by harassment incidental to... SGRLPS to incidentally harass, by Level B harassment only, four species of marine mammals during the... Commerce to authorize, upon request, the incidental, but not intentional, taking by harassment of...

  20. 75 FR 17382 - Small Takes of Marine Mammals Incidental to Specified Activities; Russian River Estuary Water...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-06

    ... Federal Register (74 FR 58248) for the take of marine mammals incidental to Estuary water level management... notice (74 FR 58248). In summary, harbor seals are the most abundant marine mammal found at the mouth of... published on November 12, 2009 (74 FR 58248). During the 30-day public comment period, six members of...

  1. 75 FR 42698 - Takes of Marine Mammals Incidental to Specified Activities; Installation of Meteorological Data...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... Mexico stock(s). Because these species are difficult to differentiate at sea, seasonal abundance...). Potential Effects on Marine Mammals NMFS has preliminarily determined that open-water impact pile driving of... throughout the world's oceans. Marine mammals produce sounds in various contexts and use sound for...

  2. Design of low energy bunch compressors with space charge effects

    NASA Astrophysics Data System (ADS)

    He, A.; Willeke, F.; Yu, L. H.; Yang, L.; Shaftan, T.; Wang, G.; Li, Y.; Hidaka, Y.; Qiang, J.

    2015-01-01

    In this paper, we explore a method to manipulate low energy electron bunches in a space charge dominated regime, and we use this method to design low energy linac bunch compressors to compress electron bunches in a space charge dominated regime. In the method, we use the space charge effects instead of avoiding them; i.e., we use the space charge forces to generate the required energy chirp instead of the ordinary method which uses the rf accelerating system to generate the chirp. We redefine the concepts of the dispersion function and beta functions in a space charge dominated regime to guide the optimization. Using this method, we study the low energy (5-22 MeV) linac bunch compressor design to produce short (˜150 fs ) and small size (˜30 μ m ) bunches for the electron beam slicing project. The low energy linac bunch compressors work in a space charge dominated regime, and the bunches at the downstream of the gun have a negative energy chirp due to the space charge effects. To provide compression for the negative energy chirped bunch, we design a positive R56 dispersive section using a four-dipole chicane with several quadrupole magnets. We have designed low energy linac bunch compressors with different photocathode rf guns. For example, one linac bunch compressor with the BNL photocathode electron rf gun has achieved a low energy bunch with the 166 fs rms bunch length, 28 and 31 μ m rms beam size in the vertical and horizontal directions, respectively, at 5 MeV with 50 pC charge. Another example with LBNL's very-high frequency gun has achieved a low energy bunch with the 128 fs rms bunch length, 42 and 25 μ m rms beam size in the vertical and horizontal directions, respectively, at 22 MeV with 200 pC charge.

  3. Recombinant production and characterization of a highly active alkaline phosphatase from marine bacterium Cobetia marina.

    PubMed

    Golotin, Vasily; Balabanova, Larissa; Likhatskaya, Galina; Rasskazov, Valery

    2015-04-01

    The psychrophilic marine bacterium, Cobetia marina, recovered from the mantle tissue of the marine mussel, Crenomytilus grayanus, which contained a gene encoding alkaline phosphatase (AP) with apparent biotechnology advantages. The enzyme was found to be more efficient than its counterparts and showed k cat value 10- to 100-fold higher than those of all known commercial APs. The enzyme did not require the presence of exogenous divalent cations and dimeric state of its molecule for activity. The recombinant enzyme (CmAP) production and purification were optimized with a final recovery of 2 mg of the homogenous protein from 1 L of the transgenic Escherichia coli Rosetta(DE3)/Pho40 cells culture. CmAP displayed a half-life of 16 min at 45 °C and 27 min at 40 °C in the presence of 2 mM EDTA, thus suggesting its relative thermostability in comparison with the known cold-adapted analogues. A high concentration of EDTA in the incubation mixture did not appreciably inhibit CmAP. The enzyme was stable in a wide range of pH (6.0-11.0). CmAP exhibited its highest activity at the reaction temperature of 40-50 °C and pH 9.5-10.3. The structural features of CmAP could be the reason for the increase in its stability and catalytic turnover. We have modeled the CmAP 3D structure on the base of the high-quality experimental structure of the close homologue Vibrio sp. AP (VAP) and mutated essential residues predicted to break Mg(2+) bonds in CmAP. It seems probable that the intrinsically tight binding of catalytic and structural metal ions together with the flexibility of intermolecular and intramolecular links in CmAP could be attributed to the adapted mutualistic lifestyle in oceanic waters. PMID:25260971

  4. Nuclear Astrophysics Programs with Low-Energy RI Beams at CRIB

    SciTech Connect

    Kubono, S.; Binh, Dam N.; Hayakawa, S.; Hashimoto, T.; Kahl, D. M.; Yamaguchi, H.; Wakabayashi, Y.; Teranishi, T.; Iwasa, N.; Komatsubara, T.; Kato, S.; Khiem, Le H.

    2010-04-30

    Nuclear astrophysics activities with CNS RI beam separator (CRIB) are reported together with the present status of the CRIB facility which is supported by the AVF upgrade project for the total development of the low-energy RIB facility. The activities include direct and indirect measurements of stellar reactions especially relevant to explosive burning processes such as nova and supernovae. Some recent results are discussed together with a scope of the facility.

  5. Nuclear Astrophysics Programs with Low-Energy RI Beams at CRIB

    NASA Astrophysics Data System (ADS)

    Kubono, S.; Binh, Dam N.; Hayakawa, S.; Hashimoto, T.; Kahl, D. M.; Yamaguchi, H.; Wakabayashi, Y.; Teranishi, T.; Iwasa, N.; Komatsubara, T.; Kato, S.; Khiem, Le H.

    2010-04-01

    Nuclear astrophysics activities with CNS RI beam separator (CRIB) are reported together with the present status of the CRIB facility which is supported by the AVF upgrade project for the total development of the low-energy RIB facility. The activities include direct and indirect measurements of stellar reactions especially relevant to explosive burning processes such as nova and supernovae. Some recent results are discussed together with a scope of the facility.

  6. Marine pharmacology in 2003-4: Marine Compounds with Anthelminthic, Antibacterial, Anticoagulant, Antifungal, Anti-inflammatory, Antimalarial, Antiplatelet, Antiprotozoal, Antituberculosis, and Antiviral Activities; affecting the Cardiovascular, Immune and Nervous Systems, and other Miscellaneous Mechanisms of Action

    PubMed Central

    Mayer, Alejandro M.S.; Rodriguez, Abimael D.; Berlinck, Roberto G.S.; Hamann, Mark T.

    2007-01-01

    The current marine pharmacology review that covers the peer-reviewed literature during 2003 and 2004 is a sequel to the authors' 1998-2002 reviews, and highlights the preclinical pharmacology of 166 marine chemicals derived from a diverse group of marine animals, algae, fungi and bacteria. Anthelminthic, antibacterial, anticoagulant, antifungal, antimalarial, antiplatelet, antiprotozoal, antituberculosis or antiviral activities were reported for 67 marine chemicals. Additionally 45 marine compounds were shown to have significant effects on the cardiovascular, immune and nervous system as well as possessing anti-inflammatory effects. Finally, 54 marine compounds were reported to act on a variety of molecular targets and thus may potentially contribute to several pharmacological classes. Thus, during 2003-2004, research on the pharmacology of marine natural products which involved investigators from Argentina, Australia, Brazil, Belgium, Canada, China, France, Germany, India, Indonesia, Israel, Italy, Japan, Mexico, Morocco, the Netherlands, New Zealand, Norway, Panama, the Philippines, Portugal, Russia, Slovenia, South Korea, Spain, Thailand, Turkey, United Kingdom, and the United States, contributed numerous chemical leads for the continued global search for novel therapeutic agents with broad spectrum activity. PMID:17392033

  7. 78 FR 44539 - Takes of Marine Mammals Incidental to Specified Activities; Taking Marine Mammals Incidental to a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-24

    ... ] notice of proposed authorization (78 FR 30873; May 23, 2013; hereafter, the FR notice); please see that... activities described herein and in the FR notice. Pier demolition and construction and relocation of the MMP... of sound sources in general was provided in the FR notice (78 FR 30873; May 23, 2013)....

  8. 78 FR 42042 - Taking of Marine Mammals Incidental to Specified Activities; U.S. Marine Corps Training Exercises...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-15

    ... Corps for the conduct of the same activities from 2010 to 2012 (75 FR 72807, November 26, 2010; 77 FR... the purpose of the program in a previous notice for the proposed Authorization (78 FR 19224, Friday, March 29, 2013). Refer to the notice of the proposed Authorization (78 FR 19224, Friday, March 29,...

  9. Exploring the chemodiversity and biological activities of the secondary metabolites from the marine fungus Neosartorya pseudofischeri.

    PubMed

    Liang, Wan-Ling; Le, Xiu; Li, Hou-Jin; Yang, Xiang-Ling; Chen, Jun-Xiong; Xu, Jun; Liu, Huan-Liang; Wang, Lai-You; Wang, Kun-Teng; Hu, Kun-Chao; Yang, De-Po; Lan, Wen-Jian

    2014-11-01

    The production of fungal metabolites can be remarkably influenced by various cultivation parameters. To explore the biosynthetic potentials of the marine fungus, Neosartorya pseudofischeri, which was isolated from the inner tissue of starfish Acanthaster planci, glycerol-peptone-yeast extract (GlyPY) and glucose-peptone-yeast extract (GluPY) media were used to culture this fungus. When cultured in GlyPY medium, this fungus produced two novel diketopiperazines, neosartins A and B (1 and 2), together with six biogenetically-related known diketopiperazines,1,2,3,4-tetrahydro-2, 3-dimethyl-1,4-dioxopyrazino[1,2-a]indole (3), 1,2,3,4-tetrahydro-2-methyl-3-methylen e-1,4-dioxopyrazino[1,2-a]indole (4), 1,2,3,4-tetrahydro-2-methyl-1,3,4-trioxopyrazino[1,2-a] indole (5), 6-acetylbis(methylthio)gliotoxin (10), bisdethiobis(methylthio)gliotoxin (11), didehydrobisdethiobis(methylthio)gliotoxin (12) and N-methyl-1H-indole-2-carboxamide (6). However, a novel tetracyclic-fused alkaloid, neosartin C (14), a meroterpenoid, pyripyropene A (15), gliotoxin (7) and five known gliotoxin analogues, acetylgliotoxin (8), reduced gliotoxin (9), 6-acetylbis(methylthio)gliotoxin (10), bisdethiobis(methylthio) gliotoxin (11) and bis-N-norgliovictin (13), were obtained when grown in glucose-containing medium (GluPY medium). This is the first report of compounds 3, 4, 6, 9, 10 and 12 as naturally occurring. Their structures were determined mainly by MS, 1D and 2D NMR data. The possible biosynthetic pathways of gliotoxin-related analogues and neosartin C were proposed. The antibacterial activity of compounds 2-14 and the cytotoxic activity of compounds 4, 5 and 7-13 were evaluated. Their structure-activity relationships are also preliminarily discussed. PMID:25421322

  10. Exploring the Chemodiversity and Biological Activities of the Secondary Metabolites from the Marine Fungus Neosartorya pseudofischeri

    PubMed Central

    Liang, Wan-Ling; Le, Xiu; Li, Hou-Jin; Yang, Xiang-Ling; Chen, Jun-Xiong; Xu, Jun; Liu, Huan-Liang; Wang, Lai-You; Wang, Kun-Teng; Hu, Kun-Chao; Yang, De-Po; Lan, Wen-Jian

    2014-01-01

    The production of fungal metabolites can be remarkably influenced by various cultivation parameters. To explore the biosynthetic potentials of the marine fungus, Neosartorya pseudofischeri, which was isolated from the inner tissue of starfish Acanthaster planci, glycerol-peptone-yeast extract (GlyPY) and glucose-peptone-yeast extract (GluPY) media were used to culture this fungus. When cultured in GlyPY medium, this fungus produced two novel diketopiperazines, neosartins A and B (1 and 2), together with six biogenetically-related known diketopiperazines,1,2,3,4-tetrahydro-2,3-dimethyl-1,4-dioxopyrazino[1,2-a]indole (3), 1,2,3,4-tetrahydro-2-methyl-3-methylene-1,4-dioxopyrazino[1,2-a]indole (4), 1,2,3,4-tetrahydro-2-methyl-1,3,4-trioxopyrazino[1,2-a] indole (5), 6-acetylbis(methylthio)gliotoxin (10), bisdethiobis(methylthio)gliotoxin (11), didehydrobisdethiobis(methylthio)gliotoxin (12) and N-methyl-1H-indole-2-carboxamide (6). However, a novel tetracyclic-fused alkaloid, neosartin C (14), a meroterpenoid, pyripyropene A (15), gliotoxin (7) and five known gliotoxin analogues, acetylgliotoxin (8), reduced gliotoxin (9), 6-acetylbis(methylthio)gliotoxin (10), bisdethiobis(methylthio) gliotoxin (11) and bis-N-norgliovictin (13), were obtained when grown in glucose-containing medium (GluPY medium). This is the first report of compounds 3, 4, 6, 9, 10 and 12 as naturally occurring. Their structures were determined mainly by MS, 1D and 2D NMR data. The possible biosynthetic pathways of gliotoxin-related analogues and neosartin C were proposed. The antibacterial activity of compounds 2–14 and the cytotoxic activity of compounds 4, 5 and 7–13 were evaluated. Their structure-activity relationships are also preliminarily discussed. PMID:25421322

  11. Purine nucleoside phosphorylase and xanthine oxidase activities in erythrocytes and plasma from marine, semiaquatic and terrestrial mammals.

    PubMed

    López-Cruz, Roberto I; Pérez-Milicua, Myrna Barjau; Crocker, Daniel E; Gaxiola-Robles, Ramón; Bernal-Vertiz, Jaime A; de la Rosa, Alejandro; Vázquez-Medina, José P; Zenteno-Savín, Tania

    2014-05-01

    Purine nucleoside phosphorylase (PNP) and xanthine oxidase (XO) are key enzymes involved in the purine salvage pathway. PNP metabolizes purine bases to synthetize purine nucleotides whereas XO catalyzes the oxidation of purines to uric acid. In humans, PNP activity is reported to be high in erythrocytes and XO activity to be low in plasma; however, XO activity increases after ischemic events. XO activity in plasma of northern elephant seals has been reported during prolonged fasting and rest and voluntary associated apneas. The objective of this study was to analyze circulating PNP and XO activities in marine mammals adapted to tolerate repeated cycles of ischemia/reperfusion associated with diving (bottlenose dolphin, northern elephant seal) in comparison with semiaquatic (river otter) and terrestrial mammals (human, pig). PNP activities in plasma and erythrocytes, as well as XO activity in plasma, from all species were quantified by spectrophotometry. No clear relationship in circulating PNP or XO activity could be established between marine, semiaquatic and terrestrial mammals. Erythrocytes from bottlenose dolphins and humans are highly permeable to nucleosides and glucose, intraerythrocyte PNP activity may be related to a release of purine nucleotides from the liver. High-energy costs will probably mean a higher ATP degradation rate in river otters, as compared to northern elephant seals or dolphins. Lower erythrocyte PNP activity and elevated plasma XO activity in northern elephant seal could be associated with fasting and/or sleep- and dive-associated apneas.

  12. Purine nucleoside phosphorylase and xanthine oxidase activities in erythrocytes and plasma from marine, semiaquatic and terrestrial mammals.

    PubMed

    López-Cruz, Roberto I; Pérez-Milicua, Myrna Barjau; Crocker, Daniel E; Gaxiola-Robles, Ramón; Bernal-Vertiz, Jaime A; de la Rosa, Alejandro; Vázquez-Medina, José P; Zenteno-Savín, Tania

    2014-05-01

    Purine nucleoside phosphorylase (PNP) and xanthine oxidase (XO) are key enzymes involved in the purine salvage pathway. PNP metabolizes purine bases to synthetize purine nucleotides whereas XO catalyzes the oxidation of purines to uric acid. In humans, PNP activity is reported to be high in erythrocytes and XO activity to be low in plasma; however, XO activity increases after ischemic events. XO activity in plasma of northern elephant seals has been reported during prolonged fasting and rest and voluntary associated apneas. The objective of this study was to analyze circulating PNP and XO activities in marine mammals adapted to tolerate repeated cycles of ischemia/reperfusion associated with diving (bottlenose dolphin, northern elephant seal) in comparison with semiaquatic (river otter) and terrestrial mammals (human, pig). PNP activities in plasma and erythrocytes, as well as XO activity in plasma, from all species were quantified by spectrophotometry. No clear relationship in circulating PNP or XO activity could be established between marine, semiaquatic and terrestrial mammals. Erythrocytes from bottlenose dolphins and humans are highly permeable to nucleosides and glucose, intraerythrocyte PNP activity may be related to a release of purine nucleotides from the liver. High-energy costs will probably mean a higher ATP degradation rate in river otters, as compared to northern elephant seals or dolphins. Lower erythrocyte PNP activity and elevated plasma XO activity in northern elephant seal could be associated with fasting and/or sleep- and dive-associated apneas. PMID:24530799

  13. Bacterial exopolysaccharides from extreme marine habitats: production, characterization and biological activities.

    PubMed

    Poli, Annarita; Anzelmo, Gianluca; Nicolaus, Barbara

    2010-01-01

    Many marine bacteria produce exopolysaccharides (EPS) as a strategy for growth, adhering to solid surfaces, and to survive adverse conditions. There is growing interest in isolating new EPS producing bacteria from marine environments, particularly from extreme marine environments such as deep-sea hydrothermal vents characterized by high pressure and temperature and heavy metal presence. Marine EPS-producing microorganisms have been also isolated from several extreme niches such as the cold marine environments typically of Arctic and Antarctic sea ice, characterized by low temperature and low nutrient concentration, and the hypersaline marine environment found in a wide variety of aquatic and terrestrial ecosystems such as salt lakes and salterns. Most of their EPSs are heteropolysaccharides containing three or four different monosaccharides arranged in groups of 10 or less to form the repeating units. These polymers are often linear with an average molecular weight ranging from 1 x 10(5) to 3 x 10(5) Da. Some EPS are neutral macromolecules, but the majority of them are polyanionic for the presence of uronic acids or ketal-linked pyruvate or inorganic residues such as phosphate or sulfate. EPSs, forming a layer surrounding the cell, provide an effective protection against high or low temperature and salinity, or against possible predators. By examining their structure and chemical-physical characteristics it is possible to gain insight into their commercial application, and they are employed in several industries. Indeed EPSs produced by microorganisms from extreme habitats show biotechnological promise ranging from pharmaceutical industries, for their immunomodulatory and antiviral effects, bone regeneration and cicatrizing capacity, to food-processing industries for their peculiar gelling and thickening properties. Moreover, some EPSs are employed as biosurfactants and in detoxification mechanisms of petrochemical oil-polluted areas. The aim of this paper is to

  14. Bacterial Exopolysaccharides from Extreme Marine Habitats: Production, Characterization and Biological Activities

    PubMed Central

    Poli, Annarita; Anzelmo, Gianluca; Nicolaus, Barbara

    2010-01-01

    Many marine bacteria produce exopolysaccharides (EPS) as a strategy for growth, adhering to solid surfaces, and to survive adverse conditions. There is growing interest in isolating new EPS producing bacteria from marine environments, particularly from extreme marine environments such as deep-sea hydrothermal vents characterized by high pressure and temperature and heavy metal presence. Marine EPS-producing microorganisms have been also isolated from several extreme niches such as the cold marine environments typically of Arctic and Antarctic sea ice, characterized by low temperature and low nutrient concentration, and the hypersaline marine environment found in a wide variety of aquatic and terrestrial ecosystems such as salt lakes and salterns. Most of their EPSs are heteropolysaccharides containing three or four different monosaccharides arranged in groups of 10 or less to form the repeating units. These polymers are often linear with an average molecular weight ranging from 1 × 105 to 3 × 105 Da. Some EPS are neutral macromolecules, but the majority of them are polyanionic for the presence of uronic acids or ketal-linked pyruvate or inorganic residues such as phosphate or sulfate. EPSs, forming a layer surrounding the cell, provide an effective protection against high or low temperature and salinity, or against possible predators. By examining their structure and chemical-physical characteristics it is possible to gain insight into their commercial application, and they are employed in several industries. Indeed EPSs produced by microorganisms from extreme habitats show biotechnological promise ranging from pharmaceutical industries, for their immunomodulatory and antiviral effects, bone regeneration and cicatrizing capacity, to food-processing industries for their peculiar gelling and thickening properties. Moreover, some EPSs are employed as biosurfactants and in detoxification mechanisms of petrochemical oil-polluted areas. The aim of this paper is to give

  15. Neutron activation analysis of stoney spherules from a marine sediment sample

    NASA Technical Reports Server (NTRS)

    Millar, H. T., Jr.; Englert, P.

    1984-01-01

    The identification of extraterrestrial material in samples collected at the surface of the Earth is discussed. Criteria were established for black magnetic spherules which involve the presence of: Fe, Ni, and Co in iron meteoritic ratios, wustite, and Fe-Ni metal while reliable criteria for stoney spherules are not well established. Neutron activation analysis was performed on eight stony spherules separated from the same marine sediment used by Millard and Finkelman. The 22 elements were determined by Compton suppression and triple coincidence gamma counting. It is found that Fe, Mg, Al, Ni, Cr, Co, Ir, and Sc are the best discriminators between chondritic and terrestrial compositions. Three of the spherules have compositions very close to chondrites and of these, two contain 0.5 and 0.25 ppm Ir. The other five spherules contain much less than chondritic concentrations of Ni but this element may be segregated and lost during ablation of the parent meteorite. One of these five low Ni spherules contains 2.9 ppm Ir while the other four contain less than 0.05 ppm Ir.

  16. Rare earth elements in core marine sediments of coastal East Malaysia by instrumental neutron activation analysis.

    PubMed

    Ashraf, Ahmadreza; Saion, Elias; Gharibshahi, Elham; Kamari, Halimah Mohamed; Kong, Yap Chee; Hamzah, Mohd Suhaimi; Elias, Md Suhaimi

    2016-01-01

    A study was carried out on the concentration of REEs (Dy, Sm, Eu,Yb, Lu, La and Ce) that are present in the core marine sediments of East Malaysia from three locations at South China Sea and one location each at Sulu Sea and Sulawesi Sea. The sediment samples were collected at a depth of between 49 and 109 m, dried, and crushed to powdery form. The entire core sediments prepared for Instrumental Neutron Activation Analysis (INAA) were weighted approximately 0.0500 g to 0.1000 g for short irradiation and 0.1500 g to 0.2000 g for long irradiation. The samples were irradiated with a thermal neutron flux of 4.0×10(12) cm(-2) s(-1) in a TRIGA Mark II research reactor operated at 750 kW. Blank samples and standard reference materials SL-1 were also irradiated for calibration and quality control purposes. It was found that the concentration of REEs varies in the range from 0.11 to 36.84 mg/kg. The chondrite-normalized REEs for different stations suggest that all the REEs are from similar origins. There was no significant REEs contamination as the enrichment factors normalized for Fe fall in the range of 0.42-2.82. PMID:26405840

  17. Mode of action of thiocoraline, a natural marine compound with anti-tumour activity

    PubMed Central

    Erba, E; Bergamaschi, D; Ronzoni, S; Faretta, M; Taverna, S; Bonfanti, M; Catapano, C V; Faircloth, G; Jimeno, J; D'Incalci, M

    1999-01-01

    Thiocoraline, a new anticancer agent derived from the marine actinomycete Micromonospora marina, was found to induce profound perturbations of the cell cycle. On both LoVo and SW620 human colon cancer cell lines, thiocoraline caused an arrest in G1 phase of the cell cycle and a decrease in the rate of S phase progression towards G2/M phases, as assessed by using bromodeoxyuridine/DNA biparametric flow cytometric analysis. Thiocoraline does not inhibit DNA-topoisomerase II enzymes in vitro, nor does it induce DNA breakage in cells exposed to effective drug concentrations. The cell cycle effects observed after exposure to thiocoraline appear related to the inhibition of DNA replication. By using a primer extension assay it was found that thiocoraline inhibited DNA elongation by DNA polymerase α at concentrations that inhibited cell cycle progression and clonogenicity. These studies indicate that the new anticancer drug thiocoraline probably acts by inhibiting DNA polymerase α activity. © 1999 Cancer Research Campaign PMID:10362104

  18. Antimicrobial Activity of Heterotrophic Bacterial Communities from the Marine Sponge Erylus discophorus (Astrophorida, Geodiidae)

    PubMed Central

    Graça, Ana Patrícia; Bondoso, Joana; Gaspar, Helena; Xavier, Joana R.; Monteiro, Maria Cândida; de la Cruz, Mercedes; Oves-Costales, Daniel; Vicente, Francisca; Lage, Olga Maria

    2013-01-01

    Heterotrophic bacteria associated with two specimens of the marine sponge Erylus discophorus were screened for their capacity to produce bioactive compounds against a panel of human pathogens (Staphylococcus aureus wild type and methicillin-resistant S. aureus (MRSA), Bacillus subtilis, Pseudomonas aeruginosa, Acinetobacter baumanii, Candida albicans and Aspergillus fumigatus), fish pathogen (Aliivibrio fischeri) and environmentally relevant bacteria (Vibrio harveyi). The sponges were collected in Berlengas Islands, Portugal. Of the 212 isolated heterotrophic bacteria belonging to Alpha- and Gammaproteobacteria, Actinobacteria and Firmicutes, 31% produced antimicrobial metabolites. Bioactivity was found against both Gram positive and Gram negative and clinically and environmentally relevant target microorganisms. Bioactivity was found mainly against B. subtilis and some bioactivity against S. aureus MRSA, V. harveyi and A. fisheri. No antifungal activity was detected. The three most bioactive genera were Pseudovibrio (47.0%), Vibrio (22.7%) and Bacillus (7.6%). Other less bioactive genera were Labrenzia, Acinetobacter, Microbulbifer, Pseudomonas, Gordonia, Microbacterium, Micrococcus and Mycobacterium, Paenibacillus and Staphylococcus. The search of polyketide I synthases (PKS-I) and nonribosomal peptide synthetases (NRPSs) genes in 59 of the bioactive bacteria suggested the presence of PKS-I in 12 strains, NRPS in 3 strains and both genes in 3 strains. Our results show the potential of the bacterial community associated with Erylus discophorus sponges as producers of bioactive compounds. PMID:24236081

  19. Reverse transcriptase genes are highly abundant and transcriptionally active in marine plankton assemblages.

    PubMed

    Lescot, Magali; Hingamp, Pascal; Kojima, Kenji K; Villar, Emilie; Romac, Sarah; Veluchamy, Alaguraj; Boccara, Martine; Jaillon, Olivier; Iudicone, Daniele; Bowler, Chris; Wincker, Patrick; Claverie, Jean-Michel; Ogata, Hiroyuki

    2016-05-01

    Genes encoding reverse transcriptases (RTs) are found in most eukaryotes, often as a component of retrotransposons, as well as in retroviruses and in prokaryotic retroelements. We investigated the abundance, classification and transcriptional status of RTs based on Tara Oceans marine metagenomes and metatranscriptomes encompassing a wide organism size range. Our analyses revealed that RTs predominate large-size fraction metagenomes (>5 μm), where they reached a maximum of 13.5% of the total gene abundance. Metagenomic RTs were widely distributed across the phylogeny of known RTs, but many belonged to previously uncharacterized clades. Metatranscriptomic RTs showed distinct abundance patterns across samples compared with metagenomic RTs. The relative abundances of viral and bacterial RTs among identified RT sequences were higher in metatranscriptomes than in metagenomes and these sequences were detected in all metatranscriptome size fractions. Overall, these observations suggest an active proliferation of various RT-assisted elements, which could be involved in genome evolution or adaptive processes of plankton assemblage.

  20. Rare earth elements in core marine sediments of coastal East Malaysia by instrumental neutron activation analysis.

    PubMed

    Ashraf, Ahmadreza; Saion, Elias; Gharibshahi, Elham; Kamari, Halimah Mohamed; Kong, Yap Chee; Hamzah, Mohd Suhaimi; Elias, Md Suhaimi

    2016-01-01

    A study was carried out on the concentration of REEs (Dy, Sm, Eu,Yb, Lu, La and Ce) that are present in the core marine sediments of East Malaysia from three locations at South China Sea and one location each at Sulu Sea and Sulawesi Sea. The sediment samples were collected at a depth of between 49 and 109 m, dried, and crushed to powdery form. The entire core sediments prepared for Instrumental Neutron Activation Analysis (INAA) were weighted approximately 0.0500 g to 0.1000 g for short irradiation and 0.1500 g to 0.2000 g for long irradiation. The samples were irradiated with a thermal neutron flux of 4.0×10(12) cm(-2) s(-1) in a TRIGA Mark II research reactor operated at 750 kW. Blank samples and standard reference materials SL-1 were also irradiated for calibration and quality control purposes. It was found that the concentration of REEs varies in the range from 0.11 to 36.84 mg/kg. The chondrite-normalized REEs for different stations suggest that all the REEs are from similar origins. There was no significant REEs contamination as the enrichment factors normalized for Fe fall in the range of 0.42-2.82.

  1. Comparison of marine gas hydrates in sediments of an active and passive continental margin

    USGS Publications Warehouse

    Kvenvolden, K.A.

    1985-01-01

    Two sites of the Deep Sea Drilling Project in contrasting geologic settings provide a basis for comparison of the geochemical conditions associated with marine gas hydrates in continental margin sediments. Site 533 is located at 3191 m water depth on a spit-like extension of the continental rise on a passive margin in the Atlantic Ocean. Site 568, at 2031 m water depth, is in upper slope sediment of an active accretionary margin in the Pacific Ocean. Both sites are characterized by high rates of sedimentation, and the organic carbon contents of these sediments generally exceed 0.5%. Anomalous seismic reflections that transgress sedimentary structures and parallel the seafloor, suggested the presence of gas hydrates at both sites, and, during coring, small samples of gas hydrate were recovered at subbottom depths of 238m (Site 533) and 404 m (Site 568). The principal gaseous components of the gas hydrates wer methane, ethane, and CO2. Residual methane in sediments at both sites usually exceeded 10 mll-1 of wet sediment. Carbon isotopic compositions of methane, CO2, and ??CO2 followed parallel trends with depth, suggesting that methane formed mainly as a result of biological reduction of oxidized carbon. Salinity of pore waters decreased with depth, a likely result of gas hydrate formation. These geochemical characteristics define some of the conditions associated with the occurrence of gas hydrates formed by in situ processes in continental margin sediments. ?? 1984.

  2. Reverse transcriptase genes are highly abundant and transcriptionally active in marine plankton assemblages

    PubMed Central

    Lescot, Magali; Hingamp, Pascal; Kojima, Kenji K; Villar, Emilie; Romac, Sarah; Veluchamy, Alaguraj; Boccara, Martine; Jaillon, Olivier; Iudicone, Daniele; Bowler, Chris; Wincker, Patrick; Claverie, Jean-Michel; Ogata, Hiroyuki

    2016-01-01

    Genes encoding reverse transcriptases (RTs) are found in most eukaryotes, often as a component of retrotransposons, as well as in retroviruses and in prokaryotic retroelements. We investigated the abundance, classification and transcriptional status of RTs based on Tara Oceans marine metagenomes and metatranscriptomes encompassing a wide organism size range. Our analyses revealed that RTs predominate large-size fraction metagenomes (>5 μm), where they reached a maximum of 13.5% of the total gene abundance. Metagenomic RTs were widely distributed across the phylogeny of known RTs, but many belonged to previously uncharacterized clades. Metatranscriptomic RTs showed distinct abundance patterns across samples compared with metagenomic RTs. The relative abundances of viral and bacterial RTs among identified RT sequences were higher in metatranscriptomes than in metagenomes and these sequences were detected in all metatranscriptome size fractions. Overall, these observations suggest an active proliferation of various RT-assisted elements, which could be involved in genome evolution or adaptive processes of plankton assemblage. PMID:26613339

  3. Global distribution and surface activity of macromolecules in offline simulations of marine organic chemistry

    DOE PAGES

    Ogunro, Oluwaseun O.; Burrows, Susannah M.; Elliott, Scott; Frossard, Amanda A.; Hoffman, Forrest M.; Letscher, Robert T.; Moore, J. Keith; Russell, Lynn M.; Wang, Shanlin; Wingenter, Oliver W.

    2015-10-13

    Here, organic macromolecules constitute high percentage components of remote sea spray. They enter the atmosphere through adsorption onto bubbles followed by bursting at the ocean surface, and go on to influence the chemistry of the fine mode aerosol. We present a global estimate of mixed-layer organic macromolecular distributions, driven by offline marine systems model output. The approach permits estimation of oceanic concentrations and bubble film surface coverages for several classes of organic compound. Mixed layer levels are computed from the output of a global ocean biogeochemistry model by relating the macromolecules to standard biogeochemical tracers. Steady state is assumed formore » labile forms, and for longer-lived components we rely on ratios to existing transported variables. Adsorption is then represented through conventional Langmuir isotherms, with equilibria deduced from laboratory analogs. Open water concentrations locally exceed one micromolar carbon for the total of protein, polysaccharide and refractory heteropolycondensate. The shorter-lived lipids remain confined to regions of strong biological activity. Results are evaluated against available measurements for all compound types, and agreement is generally quite reasonable. Global distributions are further estimated for both fractional coverage of bubble films at the air-water interface and the two-dimensional concentration excess. Overall, we show that macromolecular mapping provides a novel tool for the comprehension of oceanic surfactant distributions. Results may prove useful in planning field experiments and assessing the potential response of surface chemical behaviors to global change.« less

  4. Global distribution and surface activity of macromolecules in offline simulations of marine organic chemistry

    SciTech Connect

    Ogunro, Oluwaseun O.; Burrows, Susannah M.; Elliott, Scott; Frossard, Amanda A.; Hoffman, Forrest M.; Letscher, Robert T.; Moore, J. Keith; Russell, Lynn M.; Wang, Shanlin; Wingenter, Oliver W.

    2015-10-13

    Here, organic macromolecules constitute high percentage components of remote sea spray. They enter the atmosphere through adsorption onto bubbles followed by bursting at the ocean surface, and go on to influence the chemistry of the fine mode aerosol. We present a global estimate of mixed-layer organic macromolecular distributions, driven by offline marine systems model output. The approach permits estimation of oceanic concentrations and bubble film surface coverages for several classes of organic compound. Mixed layer levels are computed from the output of a global ocean biogeochemistry model by relating the macromolecules to standard biogeochemical tracers. Steady state is assumed for labile forms, and for longer-lived components we rely on ratios to existing transported variables. Adsorption is then represented through conventional Langmuir isotherms, with equilibria deduced from laboratory analogs. Open water concentrations locally exceed one micromolar carbon for the total of protein, polysaccharide and refractory heteropolycondensate. The shorter-lived lipids remain confined to regions of strong biological activity. Results are evaluated against available measurements for all compound types, and agreement is generally quite reasonable. Global distributions are further estimated for both fractional coverage of bubble films at the air-water interface and the two-dimensional concentration excess. Overall, we show that macromolecular mapping provides a novel tool for the comprehension of oceanic surfactant distributions. Results may prove useful in planning field experiments and assessing the potential response of surface chemical behaviors to global change.

  5. EVOLUTION OF THE CRAB NEBULA IN A LOW ENERGY SUPERNOVA

    SciTech Connect

    Yang, Haifeng; Chevalier, Roger A. E-mail: rac5x@virginia.edu

    2015-06-20

    The nature of the supernova leading to the Crab Nebula has long been controversial because of the low energy that is present in the observed nebula. One possibility is that there is significant energy in extended fast material around the Crab but searches for such material have not led to detections. An electron capture supernova model can plausibly account for the low energy and the observed abundances in the Crab. Here, we examine the evolution of the Crab pulsar wind nebula inside a freely expanding supernova and find that the observed properties are most consistent with a low energy event. Both the velocity and radius of the shell material, and the amount of gas swept up by the pulsar wind point to a low explosion energy (∼10{sup 50} erg). We do not favor a model in which circumstellar interaction powers the supernova luminosity near maximum light because the required mass would limit the freely expanding ejecta.

  6. Coulomb effects in low-energy nuclear fragmentation

    NASA Technical Reports Server (NTRS)

    Wilson, John W.; Chun, Sang Y.; Badavi, Francis F.; John, Sarah

    1993-01-01

    Early versions of the Langley nuclear fragmentation code NUCFRAG (and a publicly released version called HZEFRG1) assumed straight-line trajectories throughout the interaction. As a consequence, NUCFRAG and HZEFRG1 give unrealistic cross sections for large mass removal from the projectile and target at low energies. A correction for the distortion of the trajectory by the nuclear Coulomb fields is used to derive fragmentation cross sections. A simple energy-loss term is applied to estimate the energy downshifts that greatly alter the Coulomb trajectory at low energy. The results, which are far more realistic than prior versions of the code, should provide the data base for future transport calculations. The systematic behavior of charge-removal cross sections compares favorably with results from low-energy experiments.

  7. Feasibility of Electron Cooling for Low-Energy RHIC Operation

    SciTech Connect

    Fedotov,A.; Ben-Zvi, I.; Chang, X.; Kayran, D.; Litvinenko, V.; Pozdeyev, E.; Satogata, T.

    2008-04-01

    A concrete interest in running RHIC at low energies in a range of 2.5-25 GeV/nucleon total energy of a single beam has recently emerged. Providing collisions in this energy range, which in the RHIC case is termed 'low-energy' operation, will help to answer one of the key questions in the field of QCD about existence and location of a critical point on the QCD phase diagram. However, luminosity projections are relatively low for the lowest energy points of interest. Luminosity improvement can be provided with electron cooling applied directly in RHIC at low energies. This report summarizes the expected luminosity improvement with electron cooling, possible technical approaches and various limitations.

  8. The problem of low energy particle measurements in the magnetosphere

    NASA Technical Reports Server (NTRS)

    Whipple, E. C., Jr.

    1978-01-01

    The accurate measurement of low energy (less than 100 eV) particle properties in the magnetosphere has been difficult, partly because of the low density of such particles, but more particularly because of spacecraft interference effects. Some early examples of how these phenomena have affected particle measurements on an OGO spacecraft are presented. Data obtained with the UCSD particle detectors on ATS-6 are then presented showing how some of these difficulties have been partially overcome. Future measurements of low energy particles in the magnetosphere can be improved by: (1) improving the low energy resolution of detectors; (2) building electrostatically clean spacecraft; (3) controlling spacecraft potential; and (4) using auxiliary measurements, particularly wave data.

  9. Evolution of the Crab Nebula in a Low Energy Supernova

    NASA Astrophysics Data System (ADS)

    Yang, Haifeng; Chevalier, Roger A.

    2015-06-01

    The nature of the supernova leading to the Crab Nebula has long been controversial because of the low energy that is present in the observed nebula. One possibility is that there is significant energy in extended fast material around the Crab but searches for such material have not led to detections. An electron capture supernova model can plausibly account for the low energy and the observed abundances in the Crab. Here, we examine the evolution of the Crab pulsar wind nebula inside a freely expanding supernova and find that the observed properties are most consistent with a low energy event. Both the velocity and radius of the shell material, and the amount of gas swept up by the pulsar wind point to a low explosion energy (∼1050 erg). We do not favor a model in which circumstellar interaction powers the supernova luminosity near maximum light because the required mass would limit the freely expanding ejecta.

  10. Correlation among low-energy four-nucleon observables

    NASA Astrophysics Data System (ADS)

    Adhikari, Sadhan K.

    1981-07-01

    We study the correlation among s-wave low-energy four-nucleon observables by using a simplified dispersion theoretic (ND) approach. We find that the triton binding energy, its asymptotic normalization parameter, the deuteron exchange left-hand cut, and the nucleon-trinucleon (N-t) scattering length control the low-energy N-t system. The isospin one channel is insensitive to the asymptotic normalization parameter of the triton. The effective range function k δ for the isospin zero spin singlet N-t system has a pole whose position and residue are strongly correlated with the N-t scattering length. In this case the four-nucleon system (in our model) has an excited state whose binding energy and asymptotic normalization parameter are also correlated with the N-t scattering length. [NUCLEAR REACTIONS s-wave nucleon-trinucleon systems, spin-isospin channels, ND approach, low-energy correlations.

  11. Light element production by low energy nuclei from massive stars

    NASA Technical Reports Server (NTRS)

    Vangioni-Flam, E.; Casse, M.; Ramaty, R.

    1997-01-01

    The Orion complex is a source of gamma rays attributed to the de-excitation of fast carbon and oxygen nuclei excited through interactions with ambient hydrogen and helium. This has consequences for the production and evolution of light isotopes in the Galaxy, as massive stars appear as prolific sources of C-O rich low energy nuclei. The different stages of massive star evolution are considered in relation to the acceleration of nuclei to moderate energies. It is concluded that the low energy nuclear component originating from massive stars plays a larger role than the usual Galactic cosmic rays in shaping the evolution of Li-6, Be-9, B-10 and B-11, especially in the early Galactic evolution. The enhancement of the B-11/B-10 ratio observed in meteorites and in the interstellar medium is attributed to the interaction of low energy carbon nuclei with ambient H and to a lesser degree, to neutrino spallation.

  12. Low-energy physics of high-temperature superconductors

    SciTech Connect

    Emery, V.J. . Physics Dept.); Kivelson, S.A. . Dept. of Physics)

    1992-01-01

    It is argued that the low-energy properties of high temperature superconductors are dominated by the interaction between the mobile holes and a particular class of collective modes, corresponding to local large-amplitude low-energy fluctuations in the hole density. The latter are a consequence of the competition between the effects of long-range Coulomb interactions and the tendency of a low concentration of holes in an antiferromagnet to phase separate. The low-energy behavior of the system is governed by the same fixed point as the two-channel Kondo problem, which accounts for the universality'' of the properties of the cuprate superconductors. Predictions of the optical properties and the spin dynamics are compared with experiment. The pairing resonance of the two Kondo problem gives a mechanism of high temperature superconductivity with an unconventional symmetry of the order parameter.

  13. Low-energy physics of high-temperature superconductors

    SciTech Connect

    Emery, V.J.; Kivelson, S.A.

    1992-09-01

    It is argued that the low-energy properties of high temperature superconductors are dominated by the interaction between the mobile holes and a particular class of collective modes, corresponding to local large-amplitude low-energy fluctuations in the hole density. The latter are a consequence of the competition between the effects of long-range Coulomb interactions and the tendency of a low concentration of holes in an antiferromagnet to phase separate. The low-energy behavior of the system is governed by the same fixed point as the two-channel Kondo problem, which accounts for the ``universality`` of the properties of the cuprate superconductors. Predictions of the optical properties and the spin dynamics are compared with experiment. The pairing resonance of the two Kondo problem gives a mechanism of high temperature superconductivity with an unconventional symmetry of the order parameter.

  14. Enhancement of low-energy electron emission in 2D radioactive films.

    PubMed

    Pronschinske, Alex; Pedevilla, Philipp; Murphy, Colin J; Lewis, Emily A; Lucci, Felicia R; Brown, Garth; Pappas, George; Michaelides, Angelos; Sykes, E Charles H

    2015-09-01

    High-energy radiation has been used for decades; however, the role of low-energy electrons created during irradiation has only recently begun to be appreciated. Low-energy electrons are the most important component of radiation damage in biological environments because they have subcellular ranges, interact destructively with chemical bonds, and are the most abundant product of ionizing particles in tissue. However, methods for generating them locally without external stimulation do not exist. Here, we synthesize one-atom-thick films of the radioactive isotope (125)I on gold that are stable under ambient conditions. Scanning tunnelling microscopy, supported by electronic structure simulations, allows us to directly observe nuclear transmutation of individual (125)I atoms into (125)Te, and explain the surprising stability of the 2D film as it underwent radioactive decay. The metal interface geometry induces a 600% amplification of low-energy electron emission (<10 eV; ref. ) compared with atomic (125)I. This enhancement of biologically active low-energy electrons might offer a new direction for highly targeted nanoparticle therapies.

  15. Effects of Man's Activities on the Marine Environment; Committee on Commerce, United States Senate, Ninety-Fourth Congress, First Session. Committee Print.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Committee on Commerce.

    This report, prepared for the Committee on Commerce and the National Ocean Policy Study Committee, describes many aspects of water pollution, both national and international. Specific sections are devoted to: (1) assessing man's influence on the oceans, (2) activities that generate marine pollution, (3) specific marine pollutants, (4) policy for…

  16. Investigating Passively Floating and Weakly Swimming Organisms: An Activity for Beginning Marine Education Students.

    ERIC Educational Resources Information Center

    Schlenker, Richard M.

    This document presents the outline of a marine biology science unit designed to introduce students to the study and collection of plankton. Extensive details on plankton collections and a seven-item quiz are included. (SL)

  17. Tracking system analytic calibration activities for the Mariner Mars 1971 mission

    NASA Technical Reports Server (NTRS)

    Madrid, G. A.; Chao, C. C.; Fliegel, H. F.; Leavitt, R. K.; Mottinger, N. A.; Winn, F. B.; Wimberly, R. N.; Yip, K. B.; Zielenbach, J. W.

    1974-01-01

    Data covering various planning aspects of Mariner Mars 1971 mission are summarized. Data cover calibrating procedures for tracking stations, radio signal propagation in the troposphere, effects of charged particles on radio transmission, orbit calculation, and data smoothing.

  18. First hydroacoustic evidence of marine, active fluid vents in the Naples Bay continental shelf (Southern Italy)

    NASA Astrophysics Data System (ADS)

    Passaro, Salvatore; Genovese, Simona; Sacchi, Marco; Barra, Marco; Rumolo, Paola; Tamburrino, Stella; Mazzola, Salvatore; Basilone, Gualtiero; Placenti, Francesco; Aronica, Salvatore; Bonanno, Angelo

    2014-09-01

    We present the first results of a multidisciplinary research aimed at the detection and mapping of Active Fluid Vents (AFVs) at the seafloor of the Naples Bay, Italy. This segment of the Campania continental margin is characterised by severe Quaternary extension and intense volcanism at Ischia and Procida islands, the Campi Flegrei and Somma-Vesuvius volcanic complexes. High resolution hydroacoustic profilers were used to identify and localize fluid emission from the seafloor. ROV direct observation showed that each emission centre is generally composed by the coalescence of several emitting points. CTD probes showed that there are no significant gradients in temperature profiles. The results of this study include the detection and mapping of 54 fluid emission points all located in the - 71/- 158 m depth range, and spatially distributed into four main clusters. Three of the described clusters are located along the margin of a complex, toe-shaped seafloor morphology southwest of the Somma-Vesuvius, representing the shallow expression of partly buried, coalesced depositional features (namely, two flank collapses and one pyroclastic flow) associated with the Late Pleistocene activity of the volcano. The fourth AFV cluster was detected at the morphological - high, located about 8 km south of Naples (Banco della Montagna), represented by a field of volcaniclastic diapirs composed of massive pumiceous deposits originated from the Campi Flegrei intruding rising through the latest Quaternary-Holocene marine deposits. Our study suggests that the occurrence of AFV in this area could be genetically linked to the interaction between volcanic related seafloor morphologies and the main, NE striking faults present in the area, i.e. the Magnaghi-Sebeto line and the Vesuvian fault.

  19. Comparative Pharmacokinetic Properties and Antitumor Activity of the Marine HDACi Largazole and Largazole Peptide Isostere

    PubMed Central

    Pilon, John L.; Clausen, Dane J.; Hansen, Ryan J.; Lunghofer, Paul J.; Charles, Brad; Rose, Barbara J.; Thamm, Douglas H.; Gustafson, Daniel L.; Bradner, James E.; Williams, Robert M.

    2015-01-01

    Purpose Largazole is a potent class I selective HDACi natural product isolated from the marine cyanobacteria Symploca sp. The purpose of this study was to test synthetic analogs of Largazole to identify potential scaffold structural modifications that would improve the drug-like properties of this clinically relevant natural product. Methods The impact of Largazole scaffold replacements on in vitro growth inhibition, cell cycle arrest, induction of apoptosis, pharmacokinetic properties, and in vivo activity using a xenograft model were investigated. Results In vitro studies in colon, lung, and pancreatic cancer cell lines showed that pyridyl substituted Largazole analogs had low nanomolar/high-picomolar activity on cell proliferation, and induced apoptosis and cell cycle arrest at concentrations equivalent to or lower than the parent compound Largazole. Using IV bolus delivery at 5mg/kg, two compartmental pharmacokinetic modeling on the peptide isostere analog of Largazole indicated improved pharmacokinetics including AUC, CL, and Vss. In the A549 non-small cell lung carcinoma xenograft model using a dosage of 5 mg/kg administered intraperitoneally every other day, Largazole, Largazole thiol, and Largazole peptide isostere demonstrated tumor growth inhibition (TGI%) of 32, 44, and 66 percent respectively. Moreover, the decreased tumor growth rate for Largazole peptide isostere was statistically significant compared to control (p=0.002) and superior to Largazole (p=0.006). Surprisingly tumor growth inhibition in this system and treatment regimen was not observed with the potent pyridyl-based analogs. Conclusions Our results establish that replacing the depsipepitde linkage in Largazole with an amide may impart pharmacokinetic advantage and that alternative prodrug forms of largazole are feasible. PMID:25616967

  20. Scattering of low-energy neutrinos on atomic shells

    SciTech Connect

    Babič, Andrej; Šimkovic, Fedor

    2015-10-28

    We present a derivation of the total cross section for inelastic scattering of low-energy solar neutrinos and reactor antineutrinos on bound electrons, resulting in a transition of the electron to an excited state. The atomic-shell structure of various chemical elements is treated in terms of a nonrelativistic approximation. We estimate the interaction rates for modern neutrino detectors, in particular the Borexino and GEMMA experiments. We establish that in these experiments the effect can be safely neglected, but it could be accessible to future large-volume neutrino detectors with low energy threshold.

  1. Cluster structure of a low-energy resonance in tetraneutron

    SciTech Connect

    Lashko, Yu. A. Filippov, G. F.

    2008-02-15

    We theoretically investigate the possibility for a tetraneutron to exist as a low-energy resonance state. We explore a microscopic model based on the assumption that the tetraneutron can be treated as a compound system, where {sup 3}n + n and {sup 2}n + {sup 2}n coupled cluster configurations coexist. The influence of the Pauli principle on the kinetic energy of the relative motion of the neutron clusters is shown to result in their attraction. The strength of such attraction is high enough to ensure the existence of a low-energy resonance in the tetraneutron, provided that the oscillator length is large enough.

  2. Diffuse Galactic low energy gamma ray continuum emission

    NASA Technical Reports Server (NTRS)

    Skibo, J. G.; Ramaty, R.

    1993-01-01

    We investigate the origin of diffuse low-energy Galactic gamma-ray continuum down to about 30 keV. We calculate gamma-ray emission via bremsstrahlung and inverse Compton scattering by propagating an unbroken electron power law injection spectrum and employing a Galactic emmissivity model derived from COSB observations. To maintain the low energy electron population capable of producing the observed continuum via bremsstrahlung, a total power input of 4 x 10 exp 41 erg/s is required. This exceeds the total power supplied to the nuclear cosmic rays by about an order of magnitude.

  3. Strong flux of low-energy neutrons produced by thunderstorms.

    PubMed

    Gurevich, A V; Antonova, V P; Chubenko, A P; Karashtin, A N; Mitko, G G; Ptitsyn, M O; Ryabov, V A; Shepetov, A L; Shlyugaev, Yu V; Vildanova, L I; Zybin, K P

    2012-03-23

    We report here for the first time about the registration of an extraordinary high flux of low-energy neutrons generated during thunderstorms. The measured neutron count rate enhancements are directly connected with thunderstorm discharges. The low-energy neutron flux value obtained in our work is a challenge for the photonuclear channel of neutron generation in thunderstorm: the estimated value of the needed high-energy γ-ray flux is about 3 orders of magnitude higher than that one observed. PMID:22540588

  4. Electron polarimetry at low energies in Hall C at JLab

    NASA Astrophysics Data System (ADS)

    Gaskell, D.

    2013-11-01

    Although the majority of Jefferson Lab experiments require multi-GeV electron beams, there have been a few opportunities to make electron beam polarization measurements at rather low energies. This proceedings will discuss some of the practical difficulties encountered in performing electron polarimetry via Mo/ller scattering at energies on the order of a few hundred MeV. Prospects for Compton polarimetry at very low energies will also be discussed. While Mo/ller scattering is likely the preferred method for electron polarimetry at energies below 500 MeV, there are certain aspects of the polarimeter and experiment design that must be carefully considered.

  5. Towards adaptive management of the natural capital: Disentangling trade-offs among marine activities and seagrass meadows.

    PubMed

    Bas Ventín, Leticia; de Souza Troncoso, Jesús; Villasante, Sebastián

    2015-12-15

    This paper investigates the ecological, social and institutional dimensions of the synergies and trade-offs between seagrasses and human activities operating in the Natura 2000 protected site of San Simón Bay (Galicia, NW Spain). By means of a multidisciplinary approach that brings together the development of a biological inventory combined with participatory mapping processes we get key spatial and contextual understanding regarding how, where and why marine users interact with seagrasses and how seagrasses are considered in policy making. The results highlight the fisheries' reliance on seagrass meadows and the controversial links with shellfisheries. The study also reveals unresolved conflicts among those management plans that promote the protection of natural values and those responsible for the exploitation of marine resources. We conclude that the adoption of pre-planning bottom-up participatory processes is crucial for the design of realistic strategies where both seagrasses and human activities were considered as a couple system.

  6. Towards adaptive management of the natural capital: Disentangling trade-offs among marine activities and seagrass meadows.

    PubMed

    Bas Ventín, Leticia; de Souza Troncoso, Jesús; Villasante, Sebastián

    2015-12-15

    This paper investigates the ecological, social and institutional dimensions of the synergies and trade-offs between seagrasses and human activities operating in the Natura 2000 protected site of San Simón Bay (Galicia, NW Spain). By means of a multidisciplinary approach that brings together the development of a biological inventory combined with participatory mapping processes we get key spatial and contextual understanding regarding how, where and why marine users interact with seagrasses and how seagrasses are considered in policy making. The results highlight the fisheries' reliance on seagrass meadows and the controversial links with shellfisheries. The study also reveals unresolved conflicts among those management plans that promote the protection of natural values and those responsible for the exploitation of marine resources. We conclude that the adoption of pre-planning bottom-up participatory processes is crucial for the design of realistic strategies where both seagrasses and human activities were considered as a couple system. PMID:26589639

  7. Marine Pharmacology in 2000: Marine Compounds with Antibacterial, Anticoagulant, Antifungal, Anti-inflammatory, Antimalarial, Antiplatelet, Antituberculosis, and Antiviral Activities; Affecting the Cardiovascular, Immune, and Nervous Systems and Other Miscellaneous Mechanisms of Action

    PubMed Central

    Mayer, Alejandro M. S.; Hamann, Mark T.

    2016-01-01

    During 2000 research on the pharmacology of marine chemicals involved investigators from Australia, Brazil, Canada, Egypt, France, Germany, India, Indonesia, Israel, Italy, Japan, the Netherlands, New Zealand, Phillipines, Singapore, Slovenia, South Korea, Spain, Sweden, Switzerland, United Kingdom, and the United States. This current review, a sequel to the authors’ 1998 and 1999 reviews, classifies 68 peer-reviewed articles on the basis of the reported preclinical pharmacologic properties of marine chemicals derived from a diverse group of marine animals, algae, fungi, and bacteria. Antibacterial, anticoagulant, antifungal, antimalarial, antiplatelet, antituberculosis, or antiviral activity was reported for 35 marine chemicals. An additional 20 marine compounds were shown to have significant effects on the cardiovascular and nervous system, and to possess anti-inflammatory or immunosuppressant properties. Finally, 23 marine compounds were reported to act on a variety of molecular targets and thus could potentially contribute to several pharmacologic classes. Thus, as in 1998 and 1999, during 2000 pharmacologic research with marine chemicals continued to contribute potentially novel chemical leads to the ongoing global search for therapeutic agents in the treatment of multiple disease categories. PMID:14583811

  8. Alotaketals A and B, sesterterpenoids from the marine sponge Hamigera species that activate the cAMP cell signaling pathway.

    PubMed

    Forestieri, Roberto; Merchant, Catherine E; de Voogd, Nicole J; Matainaho, Teatulohi; Kieffer, Timothy J; Andersen, Raymond J

    2009-11-19

    The new sesterterpenoids alotaketals A (1) and B (2) have been isolated from extracts of the marine sponge Hamigera sp. collected in Papua New Guinea. Their chemical structures were elucidated by analysis of spectroscopic data. Alotaketals A and B have the unprecedented alotane carbon skeleton, and they activate the cAMP cell signaling pathway with EC(50)'s of 18 and 240 nM, respectively. PMID:19873990

  9. [Effect of biologically active substances from marine organisms on lipid peroxidation-antioxidant defence system in gastroduodenitis, associated with hyperlipidemia].

    PubMed

    Knyshova, V V; Ivanova, I L; Kozlovskaia, E P; Loenko, Iu N

    2002-01-01

    An experimental model of gastroduodenitis combined with hyperlipidemia was used to study the effects of the product Zolotoi rog (Golden Horn) which is a composition of biologically active substances of marine organisms and honey. It was found that a course administration of Zolotoi rog in a dose 2.5 mg/kg b.w. improves histomorphology of gastric mucosa, acts hypolipidemically, raises reserves of the antioxidant system of the body and suppresses intensity of lipid peroxidation.

  10. Culturable diversity and antimicrobial activity of Actinobacteria from marine sediments in Valparaíso bay, Chile

    PubMed Central

    Claverías, Fernanda P.; Undabarrena, Agustina; González, Myriam; Seeger, Michael; Cámara, Beatriz

    2015-01-01

    Marine-derived Actinobacteria are a source of a broad variety of secondary metabolites with diverse biological activities, such as antibiotics and antitumorals; many of which have been developed for clinical use. Rare Actinobacteria represent an untapped source of new bioactive compounds that have been scarcely recognized. In this study, rare Actinobacteria from marine sediments were isolated from the Valparaíso bay, Chile, and their potential to produce antibacterial compounds was evaluated. Different culture conditions and selective media that select the growth of Actinobacteria were used leading to the isolation of 68 bacterial strains. Comparative analysis of the 16S rRNA gene sequences led to identifying isolates that belong to the phylum Actinobacteria with genetic affiliations to 17 genera: Aeromicrobium, Agrococcus, Arthrobacter, Brachybacterium, Corynebacterium, Dietzia, Flaviflexus, Gordonia, Isoptericola, Janibacter, Microbacterium, Mycobacterium, Ornithinimicrobium, Pseudonocardia, Rhodococcus, Streptomyces, and Tessaracoccus. Also, one isolate could not be consistently classified and formed a novel phylogenetic branch related to the Nocardiopsaceae family. The antimicrobial activity of these isolates was evaluated, demonstrating the capability of specific novel isolates to inhibit the growth of Gram-positive and Gram-negative bacteria. In conclusion, this study shows a rich biodiversity of culturable Actinobacteria, associated to marine sediments from Valparaíso bay, highlighting novel rare Actinobacteria, and their potential for the production of biologically active compounds. PMID:26284034

  11. Update: Exertional hyponatremia, active component, U.S. Army, Navy, Air Force, and Marine Corps, 2000-2015.

    PubMed

    2016-03-01

    From 2000 through 2015, there were 1,542 incident diagnoses of exertional hyponatremia among active component members of the U.S. Army, Navy, Air Force, and Marine Corps. Annual incidence rates rose sharply from 2008 through 2010 but then decreased by more than 50% from 2010 through 2013. In 2015, the number of cases (n=116) increased by approximately 20% from the previous year. The recent increase in rates overall reflects increased rates in the Army and the Marine Corps. Relative to their respective counterparts, crude incidence rates of exertional hyponatremia for the entire 16-year surveillance period were higher among females, those in the youngest age group, Marines, and recruit trainees. Service members (particularly recruit trainees) and their supervisors must be vigilant for early signs of heat-related illnesses and must be knowledgeable of the dangers of excessive water consumption and the prescribed limits for water intake during prolonged physical activity (e.g., field training exercises, personal fitness training, recreational activities) in hot, humid weather. PMID:27030930

  12. Culturable diversity and antimicrobial activity of Actinobacteria from marine sediments in Valparaíso bay, Chile.

    PubMed

    Claverías, Fernanda P; Undabarrena, Agustina; González, Myriam; Seeger, Michael; Cámara, Beatriz

    2015-01-01

    Marine-derived Actinobacteria are a source of a broad variety of secondary metabolites with diverse biological activities, such as antibiotics and antitumorals; many of which have been developed for clinical use. Rare Actinobacteria represent an untapped source of new bioactive compounds that have been scarcely recognized. In this study, rare Actinobacteria from marine sediments were isolated from the Valparaíso bay, Chile, and their potential to produce antibacterial compounds was evaluated. Different culture conditions and selective media that select the growth of Actinobacteria were used leading to the isolation of 68 bacterial strains. Comparative analysis of the 16S rRNA gene sequences led to identifying isolates that belong to the phylum Actinobacteria with genetic affiliations to 17 genera: Aeromicrobium, Agrococcus, Arthrobacter, Brachybacterium, Corynebacterium, Dietzia, Flaviflexus, Gordonia, Isoptericola, Janibacter, Microbacterium, Mycobacterium, Ornithinimicrobium, Pseudonocardia, Rhodococcus, Streptomyces, and Tessaracoccus. Also, one isolate could not be consistently classified and formed a novel phylogenetic branch related to the Nocardiopsaceae family. The antimicrobial activity of these isolates was evaluated, demonstrating the capability of specific novel isolates to inhibit the growth of Gram-positive and Gram-negative bacteria. In conclusion, this study shows a rich biodiversity of culturable Actinobacteria, associated to marine sediments from Valparaíso bay, highlighting novel rare Actinobacteria, and their potential for the production of biologically active compounds.

  13. Low-energy Charged Particle Measurements in the Heliosheath

    NASA Astrophysics Data System (ADS)

    Decker, R. B.

    2011-12-01

    This talk will focus on recent measurements from the LECP instruments on Voyager 1 and Voyager 2. Voyager 1 (118 AU, N34 deg.) crossed the termination shock at 94.0 AU in Dec. 2004 and is roughly 24 AU into the inner heliosheath. Voyager 2 (96 AU, S30 deg) crossed the shock at 83.5 AU in Sep. 2007 and is roughly 13 AU into the heliosheath. Large variations in the intensities, energy spectra, and angular distributions of low-energy heliosheath ions and electrons have occurred during the past year, evidently in response to an increase in the number of solar active regions in January 2010. For example, starting around 2010.8, ion intensities at Voyager 1 began a two-step exponential decrease. The intensities of ions below 0.5 MeV fell by 40%, reaching minima around 2011.5 that were the lowest measured since mid-2005. During the intensity drop at Voyager 1 the ion energy spectrum evolved to a single power-law with an index -1.5. Similar intensity drops began somewhat earlier at Voyager 2, and the intensity minima of the low-energy ions occurred on 2011.2, one-third of a year earlier than at Voyager 1. In addition, after the ion intensities had recovered at Voyager 2, they were deficient at lower energies compared to the pre-drop levels, causing the energy spectrum to flatten with decreasing energy. During the intensity drop at Voyager 2, ion angular distributions evolved from convective-like, consistent with the measured plasma flow velocity, to azimuthal, more consistent with unidirectional streaming along the mean magnetic field. We will also summarize the heliosheath plasma flow velocity in the R-T (instrument scan) plane that is estimated using angular data from the three lowest energy Voyager 1 LECP ion channels, which cover 40-139 keV. The estimated radial component of flow decreased from about 60 km/s to about 0 km/s at a rate of -19 km/s/yr during 2008.7-2010.3, was consistent with 0 km/s from 2010.3-2011.0, and then went negative, averaging about -13 km

  14. Enhanced production of low energy electrons by alpha particle impact.

    PubMed

    Kim, Hong-Keun; Titze, Jasmin; Schöffler, Markus; Trinter, Florian; Waitz, Markus; Voigtsberger, Jörg; Sann, Hendrik; Meckel, Moritz; Stuck, Christian; Lenz, Ute; Odenweller, Matthias; Neumann, Nadine; Schössler, Sven; Ullmann-Pfleger, Klaus; Ulrich, Birte; Fraga, Rui Costa; Petridis, Nikos; Metz, Daniel; Jung, Annika; Grisenti, Robert; Czasch, Achim; Jagutzki, Ottmar; Schmidt, Lothar; Jahnke, Till; Schmidt-Böcking, Horst; Dörner, Reinhard

    2011-07-19

    Radiation damage to living tissue stems not only from primary ionizing particles but to a substantial fraction from the dissociative attachment of secondary electrons with energies below the ionization threshold. We show that the emission yield of those low energy electrons increases dramatically in ion-atom collisions depending on whether or not the target atoms are isolated or embedded in an environment. Only when the atom that has been ionized and excited by the primary particle impact is in immediate proximity of another atom is a fragmentation route known as interatomic Coulombic decay (ICD) enabled. This leads to the emission of a low energy electron. Over the past decade ICD was explored in several experiments following photoionization. Most recent results show its observation even in water clusters. Here we show the quantitative role of ICD for the production of low energy electrons by ion impact, thus approaching a scenario closer to that of radiation damage by alpha particles: We choose ion energies on the maximum of the Bragg peak where energy is most efficiently deposited in tissue. We compare the electron production after colliding He(+) ions on isolated Ne atoms and on Ne dimers (Ne(2)). In the latter case the Ne atom impacted is surrounded by a most simple environment already opening ICD as a deexcitation channel. As a consequence, we find a dramatically enhanced low energy electron yield. The results suggest that ICD may have a significant influence on cell survival after exposure to ionizing radiation.

  15. Effective low-energy theory for superconducting topological insulators.

    PubMed

    Hao, Lei; Lee, Ting-Kuo

    2015-03-18

    Candidate pairings of superconducting topological insulators support interesting surface Andreev bound states (SABSs) known as Majorana fermions. As these materials are described by a two-orbital Bernevig-Hughes-Zhang type model, a general understanding of the low-energy physics such as the possible kinds of SABSs are difficult. By virtue of an analogy between a topological insulator and a time reversal invariant topological superconductor, we propose a simple and intuitive method of constructing the low-energy effective models for superconducting topological insulators like CuxBi2Se3. Depending on the value of the chemical potential and for experimentally relevant model parameters, the low-energy properties of these superconductors are shown to be determined by one copy or two copies of single-orbital effective models. If the effective pairing potential shows sign reversal upon reflection by the surface, one Kramers' pair or two Kramers' pairs of SABSs are expected to appear. Explicit analytical calculations in terms of the effective low energy model reproduce the dispersions of the numerically confirmed two pairs of SABSs for a commonly studied pairing.

  16. MEIC Proton Beam Formation with a Low Energy Linac

    SciTech Connect

    Zhang, Yuhong

    2015-09-01

    The MEIC proton and ion beams are generated, accumulated, accelerated and cooled in a new green-field ion injector complex designed specifically to support its high luminosity goal. This injector consists of sources, a linac and a small booster ring. In this paper we explore feasibility of a short ion linac that injects low-energy protons and ions into the booster ring.

  17. Nuclear phenomena in low-energy nuclear reaction research.

    PubMed

    Krivit, Steven B

    2013-09-01

    This is a comment on Storms E (2010) Status of Cold Fusion, Naturwissenschaften 97:861-881. This comment provides the following remarks to other nuclear phenomena observed in low-energy nuclear reactions aside from helium-4 make significant contributions to the overall energy balance; and normal hydrogen, not just heavy hydrogen, produces excess heat.

  18. Physics overview of the Fermilab Low Energy Antiproton Facility Workshop

    SciTech Connect

    Chanowitz, M.S.

    1986-05-01

    A physics overview is presented of the Fermilab workshop to consider a possible high flux, low energy antiproton facility that would use cooled antiprotons from the accumulator ring of the Tevatron collider. Two examples illustrate the power of each a facility to produce narrow states at high rates. Physics topics to which such a facility may be applied are reviewed.

  19. Mirrored low-energy channel for the MiniXRD

    NASA Astrophysics Data System (ADS)

    Dutra, E. C.; MacNeil, L. P.; Compton, S. M.; Jacoby, B. A.; Raphaelian, M. L.

    2015-08-01

    X-ray Diodes (XRDs) are currently used for spectroscopic measurements, measuring X-ray flux, and estimating spectral shape of the VUV to soft X-ray spectrum. A niche exists for an inexpensive, robust X-ray diode that can be used for experiments in hostile environments on multiple platforms, including explosively driven experiments that have the potential for destroying the diode during the experiment. A multiple channel stacked filtered array was developed with a small field of view where a wider parallel array could not be used, but filtered channels for energies lower than 1000 eV were too fragile to deploy under normal conditions. To achieve both the robustness and the required low-energy detection ability, we designed a small low-energy mirrored channel with a spectral sensitivity from 30 to 1000 eV. The stacked MiniXRD X-ray diode system design incorporates the mirrored low-energy channel on the front of the stacked filtered channels to allow the system to work within a small field of view. We will present results that demonstrate this is a promising solution for low-energy spectrum measurements.

  20. Sphingomonas molluscorum sp. nov., a novel marine isolate with antimicrobial activity.

    PubMed

    Romanenko, Lyudmila A; Uchino, Masataka; Frolova, Galina M; Tanaka, Naoto; Kalinovskaya, Natalia I; Latyshev, Nicolai; Mikhailov, Valery V

    2007-02-01

    An aerobic, Gram-negative, yellow-pigmented, non-motile bacterium, designated strain KMM 3882T, was isolated from a marine bivalve (Anadara broughtoni) collected from Peter the Great Bay, Sea of Japan, and was subjected to phenotypic and phylogenetic analyses. Strain KMM 3882T was found to exert a remarkable inhibitory activity against a number of Gram-positive micro-organisms. Phylogenetic analysis based on 16S rRNA gene sequences placed strain KMM 3882T within the genus Sphingomonas, as an independent lineage adjacent to Sphingomonas dokdonensis DS-4T and Sphingomonas panni DSM 15761T. Strain KMM 3882T showed the highest 16S rRNA gene sequence similarity to Sphingomonas dokdonensis DS-4T (97.3 %); similarities of 96.5-96.7 % were obtained with Sphingomonas pituitosa DSM 13101T, Sphingomonas azotifigens NBRC 15497T, Sphingomonas asaccharolytica NBRC 15499T, Sphingomonas trueperi DSM 7225T and Sphingomonas panni DSM 15761T. Chemotaxonomically, strain KMM 3882T contained sphingoglycolipid, C(16 : 0) and C(18 : 1) as predominant fatty acids and 2-OH C(14 : 0) as a major 2-hydroxy fatty acid, confirming the affiliation of strain KMM 3882T with the genus Sphingomonas. On the basis of phylogenetic analysis, DNA-DNA hybridization and physiological and biochemical characterization, strain KMM 3882T should be classified as representing a novel species of the genus Sphingomonas, for which the name Sphingomonas molluscorum sp. nov. is proposed. The type strain is KMM 3882T (=An 18T=NRIC 0685T=JCM 14122T=CIP 109223T).

  1. The variability in DMSP content and DMSP lyase activity in marine dinoflagellates

    NASA Astrophysics Data System (ADS)

    Caruana, Amandine M. N.; Malin, Gill

    2014-01-01

    More than 20 years ago Maureen Keller and co-workers published a study that identified dinoflagellates as an important marine phytoplankton group with respect to the production of dimethylsulphoniopropionate (DMSP). Here, we present a synthesis and analysis of all the DMSP and DMSP lyase activity (DLA) measurements currently available for dinoflagellates. The data cover 110 species and strains and reveal over 6 orders of magnitude variability in intracellular DMSP concentrations and substantial variations in DLA in 23 strains. Inter-specific variability was explored with reference to a range of biological characteristics. The presence of a theca did not appear to be related to DMSP concentration but there was a potential relationship with toxicity (P = 0.06) and bioluminescent species produced significantly lower concentrations (P < 0.01) than non-bioluminescent ones. DMSP concentrations were related to plastid types (P < 0.05); dinoflagellates with haptophyte-like plastids contained lower amounts of DMSP than those with peridinin plastids (P < 0.01), whereas those containing cryptomonad-like plastids tended to have higher DMSP concentrations. Heterotrophic dinoflagellates were also considered given their importance in the natural environment. They are the only heterotrophs known to synthesise DMSP and this ability may support the theory that they are of photosynthetic origin. However, the heterotrophic species investigated so far suggest wide variability in DMSP content and the species Oxyrrhis marina had no detectable DMSP. The oceanic province of origin significantly affected the DMSP concentrations (P < 0.05) with higher DMSP content observed in dinoflagellates from the Mediterranean province, the Kuroshio Current province and the East Coastal Australian province. Overall this study supports the concept that DMSP-containing dinoflagellates are an important potential source of DMS to the global atmosphere and highlights current gaps in knowledge.

  2. Extraction and PTP1B inhibitory activity of bromophenols from the marine red alga Symphyocladia latiuscula

    NASA Astrophysics Data System (ADS)

    Liu, Xu; Li, Xiaoming; Gao, Lixin; Cui, Chuanming; Li, Chunshun; Li, Jia; Wang, Bingui

    2011-05-01

    Previously, we had characterized several structurally interesting brominated phenols from the marine red alga Symphyocladia latiuscula collected from various sites. However, Phytochemical investigations on this species collected from the Weihai coastline of Shandong Province remains blank. Therefore, we characterized the chemical constituents of individuals of this species collected from the region. Eight bromophenols were isolated and identified. Using detailed spectroscopic techniques and comparisons with published data, these compounds were identified as 2,3-dibromo-4,5-dihydroxybenzyl methyl ether ( 1), 3,5-dibromo-4-hydroxybenzoic acid ( 2), 2,3,6-tribromo-4,5-dihydroxymethylbenzene ( 3), 2,3,6-tribromo-4,5-dihydroxybenzaldehyde ( 4), 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether ( 5), bis(2,3,6-tribromo-4,5-dihydroxyphenyl)methane ( 6), 1,2-bis(2,3,6-tribromo-4,5-dihydroxyphenyl)-ethane ( 7), and 1-(2,3,6-tribromo-4,5-dihydroxybenzyl)-pyrrolidin-2-one ( 8). Among these compounds, 1 and 2 were isolated for the first time from S. latiuscula. Each compound was evaluated on the ability to inhibit protein tyrosine phosphatase 1B (PTP1B), which is a potential therapeutic target in the treatment of type 2 diabetes. Bromophenols 5, 6, and 7 showed strong activities with IC50 values of 3.9, 4.3, and 3.5 μmol/L, respectively. This study provides further evidence that bromophenols are predominant among the chemical constituents of Symphyocladia, and that some of these compounds may be candidates for the development of anti-diabetes drugs.

  3. Chemoautotrophic carbon fixation rates and active bacterial communities in intertidal marine sediments.

    PubMed

    Boschker, Henricus T S; Vasquez-Cardenas, Diana; Bolhuis, Henk; Moerdijk-Poortvliet, Tanja W C; Moodley, Leon

    2014-01-01

    Chemoautotrophy has been little studied in typical coastal marine sediments, but may be an important component of carbon recycling as intense anaerobic mineralization processes in these sediments lead to accumulation of high amounts of reduced compounds, such as sulfides and ammonium. We studied chemoautotrophy by measuring dark-fixation of 13C-bicarbonate into phospholipid derived fatty acid (PLFA) biomarkers at two coastal sediment sites with contrasting sulfur chemistry in the Eastern Scheldt estuary, The Netherlands. At one site where free sulfide accumulated in the pore water right to the top of the sediment, PLFA labeling was restricted to compounds typically found in sulfur and ammonium oxidizing bacteria. At the other site, with no detectable free sulfide in the pore water, a very different PLFA labeling pattern was found with high amounts of label in branched i- and a-PLFA besides the typical compounds for sulfur and ammonium oxidizing bacteria. This suggests that other types of chemoautotrophic bacteria were also active, most likely Deltaproteobacteria related to sulfate reducers. Maximum rates of chemoautotrophy were detected in first 1 to 2 centimeters of both sediments and chemosynthetic biomass production was high ranging from 3 to 36 mmol C m(-2) d(-1). Average dark carbon fixation to sediment oxygen uptake ratios were 0.22±0.07 mol C (mol O2)(-1), which is in the range of the maximum growth yields reported for sulfur oxidizing bacteria indicating highly efficient growth. Chemoautotrophic biomass production was similar to carbon mineralization rates in the top of the free sulfide site, suggesting that chemoautotrophic bacteria could play a crucial role in the microbial food web and labeling in eukaryotic poly-unsaturated PLFA was indeed detectable. Our study shows that dark carbon fixation by chemoautotrophic bacteria is a major process in the carbon cycle of coastal sediments, and should therefore receive more attention in future studies on

  4. Chemoautotrophic carbon fixation rates and active bacterial communities in intertidal marine sediments.

    PubMed

    Boschker, Henricus T S; Vasquez-Cardenas, Diana; Bolhuis, Henk; Moerdijk-Poortvliet, Tanja W C; Moodley, Leon

    2014-01-01

    Chemoautotrophy has been little studied in typical coastal marine sediments, but may be an important component of carbon recycling as intense anaerobic mineralization processes in these sediments lead to accumulation of high amounts of reduced compounds, such as sulfides and ammonium. We studied chemoautotrophy by measuring dark-fixation of 13C-bicarbonate into phospholipid derived fatty acid (PLFA) biomarkers at two coastal sediment sites with contrasting sulfur chemistry in the Eastern Scheldt estuary, The Netherlands. At one site where free sulfide accumulated in the pore water right to the top of the sediment, PLFA labeling was restricted to compounds typically found in sulfur and ammonium oxidizing bacteria. At the other site, with no detectable free sulfide in the pore water, a very different PLFA labeling pattern was found with high amounts of label in branched i- and a-PLFA besides the typical compounds for sulfur and ammonium oxidizing bacteria. This suggests that other types of chemoautotrophic bacteria were also active, most likely Deltaproteobacteria related to sulfate reducers. Maximum rates of chemoautotrophy were detected in first 1 to 2 centimeters of both sediments and chemosynthetic biomass production was high ranging from 3 to 36 mmol C m(-2) d(-1). Average dark carbon fixation to sediment oxygen uptake ratios were 0.22±0.07 mol C (mol O2)(-1), which is in the range of the maximum growth yields reported for sulfur oxidizing bacteria indicating highly efficient growth. Chemoautotrophic biomass production was similar to carbon mineralization rates in the top of the free sulfide site, suggesting that chemoautotrophic bacteria could play a crucial role in the microbial food web and labeling in eukaryotic poly-unsaturated PLFA was indeed detectable. Our study shows that dark carbon fixation by chemoautotrophic bacteria is a major process in the carbon cycle of coastal sediments, and should therefore receive more attention in future studies on

  5. FINAL TECHNICAL REPORT: Underwater Active Acoustic Monitoring Network For Marine And Hydrokinetic Energy Projects

    SciTech Connect

    Stein, Peter J.; Edson, Patrick L.

    2013-12-20

    This project saw the completion of the design and development of a second generation, high frequency (90-120 kHz) Subsurface-Threat Detection Sonar Network (SDSN). The system was deployed, operated, and tested in Cobscook Bay, Maine near the site the Ocean Renewable Power Company TidGen™ power unit. This effort resulted in a very successful demonstration of the SDSN detection, tracking, localization, and classification capabilities in a high current, MHK environment as measured by results from the detection and tracking trials in Cobscook Bay. The new high frequency node, designed to operate outside the hearing range of a subset of marine mammals, was shown to detect and track objects of marine mammal-like target strength to ranges of approximately 500 meters. This performance range results in the SDSN system tracking objects for a significant duration - on the order of minutes - even in a tidal flow of 5-7 knots, potentially allowing time for MHK system or operator decision-making if marine mammals are present. Having demonstrated detection and tracking of synthetic targets with target strengths similar to some marine mammals, the primary hurdle to eventual automated monitoring is a dataset of actual marine mammal kinematic behavior and modifying the tracking algorithms and parameters which are currently tuned to human diver kinematics and classification.

  6. The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes.

    PubMed

    Tchigvintsev, Anatoli; Tran, Hai; Popovic, Ana; Kovacic, Filip; Brown, Greg; Flick, Robert; Hajighasemi, Mahbod; Egorova, Olga; Somody, Joseph C; Tchigvintsev, Dmitri; Khusnutdinova, Anna; Chernikova, Tatyana N; Golyshina, Olga V; Yakimov, Michail M; Savchenko, Alexei; Golyshin, Peter N; Jaeger, Karl-Erich; Yakunin, Alexander F

    2015-03-01

    Most of the Earth's biosphere is cold and is populated by cold-adapted microorganisms. To explore the natural enzyme diversity of these environments and identify new carboxylesterases, we have screened three marine metagenome gene libraries for esterase activity. The screens identified 23 unique active clones, from which five highly active esterases were selected for biochemical characterization. The purified metagenomic esterases exhibited high activity against α-naphthyl and p-nitrophenyl esters with different chain lengths. All five esterases retained high activity at 5 °C indicating that they are cold-adapted enzymes. The activity of MGS0010 increased more than two times in the presence of up to 3.5 M NaCl or KCl, whereas the other four metagenomic esterases were inhibited to various degrees by these salts. The purified enzymes showed different sensitivities to inhibition by solvents and detergents, and the activities of MGS0010, MGS0105 and MGS0109 were stimulated three to five times by the addition of glycerol. Screening of purified esterases against 89 monoester substrates revealed broad substrate profiles with a preference for different esters. The metagenomic esterases also hydrolyzed several polyester substrates including polylactic acid suggesting that they can be used for polyester depolymerization. Thus, esterases from marine metagenomes are cold-adapted enzymes exhibiting broad biochemical diversity reflecting the environmental conditions where they evolved.

  7. Isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities.

    PubMed

    Romanenko, Lyudmila A; Uchino, Masataka; Kalinovskaya, Natalia I; Mikhailov, Valery V

    2008-01-01

    This study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell Anadara broughtoni inhabiting in the Sea of Japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. A total of 149 strains were isolated and identified phenotypically. A total of 27 strains were selected to be investigated phylogenetically by 165 rRNA gene sequence analysis. The most bacteria were affiliated with members of the Gammaproteobacteria and Alphaproteobacteria, and Less with Firmicutes, Actinobacteria, and Cytophaga-Flavobacterium-Bacteroides (CFB) group. The isolates capable of hemolysis were numerically abundant in the genera Pseudoalteromonas, Aeromonas and Bacillus. The six Gram-positive isolates belonging to the genera Bacillus, Paenibacillus and Saccharothrix and two Gram-negative strains related to Pseudomonas and Sphingomonas, possessed antimicrobial activity against indicator strains and to each other. Antimicrobial, hemolytic and surface activities were revealed in butanot extracts of cells or cell-free supernatant of six active strains. This points to availability of active low-molecular-weight metabolites. Substances with hemolytic and surface activities were isolated from strain Bacillus pumilus An 112 and characterized as cyclic depsipeptides with molecular masses 1021, 1035, 1049, 1063 and 1077 Da. The recovery of strains producing antimicrobial and surface-active substances suggests that microorganisms associated with the marine bivalve are potential source of bioactive metabolites.

  8. Modification of polymer (PET) surface reactivity by low energy ion bombardment

    NASA Astrophysics Data System (ADS)

    Bertrand, P.; De Puydt, Y.; Beuken, J.-M.; Lutgen, P.; Feyder, G.

    The surface of polyethylene terephtalate (PET) was studied by low energy He + ion scattering (ISS). Modifications of the surface composition induced by the He + and Ar + bombardments are observed. The ion bombardment causes surface damages with bond breaking and it results a highly activated surface for the chemisorption of nitrogen from the residual gas phase. Similar effects were observed on highly oriented pyrolytic graphite when analyzed in the same beam conditions.

  9. The relative effect of behaviour in larval dispersal in a low energy embayment

    NASA Astrophysics Data System (ADS)

    Daigle, Rémi M.; Chassé, Joël; Metaxas, Anna

    2016-05-01

    This study examined the relative importance of tidal phase, larval behaviour, release site, depth layer, and vertical swimming velocity on mean in-sea dispersal distance, retention, distance from shore, and population connectivity. Using a biophysical model, we simulated larval dispersal of marine benthic invertebrates for 6 taxonomic groups representing different combinations of swimming speed, and depth preference in St. George's Bay, NS, Canada, a shallow bay with low energy (e.g. lack of estuarine circulation). The biophysical model was run over a period of 3 months, from Jul to Sep, representing the period when larvae of the targeted species were present, and at each of 3 years. Overall, release site had the strongest effect of all factors on the dispersal metrics. Although less important than release site in our system, vertical distribution and swim speed had a significant effect which would likely be more pronounced in high (i.e. with features such as estuarine circulation or internal waves) than low energy environments. Retention and distance from shore were more responsive to our manipulations than dispersal distance, both in terms of the number of ecologically significant effects and the magnitudes of their effect size. These findings allow for the prioritization of biophysical model parameters and improved simulations of larval dispersal.

  10. Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica

    PubMed Central

    Lemak, Sofia; Tchigvintsev, Anatoli; Petit, Pierre; Flick, Robert; Singer, Alexander U.; Brown, Greg; Evdokimova, Elena; Egorova, Olga; Gonzalez, Claudio F.; Chernikova, Tatyana N.; Yakimov, Michail M.; Kube, Michael; Reinhardt, Richard; Golyshin, Peter N.; Savchenko, Alexei; Yakunin, Alexander F.

    2014-01-01

    The uncharacterized α/β-hydrolase protein OLEI01171 from the psychrophilic marine bacterium Oleispira antarctica belongs to the PF00756 family of putative esterases, which also includes human esterase D. In the present paper we show that purified recombinant OLEI01171 exhibits high esterase activity against the model esterase substrate α-naphthyl acetate at 5 – 30°C with maximal activity at 15–20°C. The esterase activity of OLEI01171 was stimulated 3–8-fold by the addition of chloride or several other anions (0.1–1.0 M). Compared with mesophilic PF00756 esterases, OLEI01171 exhibited a lower overall protein thermostability. Two crystal structures ofOLEI01171 were solved at 1.75 and 2.1 Å resolution and revealed a classical serine hydrolase catalytic triad and the presence of a chloride or bromide ion bound in the active site close to the catalytic Ser148.Both anions were found to co-ordinate a potential catalytic water molecule located in the vicinity of the catalytic triad His257. The results of the present study suggest that the bound anion perhaps contributes to the polarization of the catalytic water molecule and increases the rate of the hydrolysis of an acyl-enzyme intermediate. Alanine replacement mutagenesis of OLEI01171 identified ten amino acid residues important for esterase activity. The replacement of Asn225 by lysine had no significant effect on the activity or thermostability of OLEI01171, but resulted in a detectable increase of activity at 35–45°C. The present study has provided insight into the molecular mechanisms of activity of a cold-active and anion-activated carboxyl esterase. PMID:22519667

  11. Monodisperse Ag@SiO2 core-shell nanoparticles as active inhibitors for marine anticorrosion applications.

    PubMed

    Zhang, Xin-Sheng; Wang, Jie-Xin; Xu, Ke; Le, Yuan; Chen, Jian-Feng

    2011-04-01

    Monodisperse Ag@SiO2 core-shell structured nanoparticles were firstly utilized as a novel corrosion inhibitor for marine anticorrosion applications. The related marine anticorrosion properties were evaluated with an electrochemical noise (ECN) analysis during 2 weeks of accelerated immersion tests in natural seawater with the addition of various inorganic salts and nutriments. The experimental results indicate that the corrosion activity is markedly reduced by nearly 1-3 orders of magnitude owing to the introduction of Ag@SiO2 core-shell nanoparticles into coating. The inhibition efficiency of corrosion can reach as high as about 99%. More importantly, such a coating exhibits an excellent long-term sustained marine anticorrosion effect. So it could be reasonably inferred that silver cores as active inhibitors effectively prevent the corrosion damage from microorganisms, while silica shells act as a good protection for silver nanoparticles, delay the release of silver ions, and also function as the corrosion inhibiting action for inorganic salts. Therefore, this would make monodisperse Ag@SiO2 core-shell nanoparticles a potential and promising corrosion inhibitor for developing future advanced multifunctional coatings.

  12. On the relation of structure, perception and activity in marine planktonic copepods

    NASA Astrophysics Data System (ADS)

    Paffenhöfer, G.-A.

    1998-06-01

    The goal of this paper is to illustrate how in juvenile and adult subtropical marine planktonic copepods various structures or morphological features function in concert to detect prey and predators. Without motion by either food (e.g. flagellate, ciliate) or feeder (e.g. feeding current) or both (e.g. Acartia spp. and ciliate) few feeding activities will occur. Through motion a food particle is either perceived mechanically or chemically to be followed by appendage activities. A combination of mechano- and chemosensors on their cephalic appendages (and probably on other extremities) serve juvenile and adult copepods to perceive signals. Perception is followed by alternation of motion and sensing by these appendages, or by no motion at all (e.g. behavior by Eucalanus pileatus when perceiving a weak hydrodynamic signal). Non-moving and extended sensors (setae) are best suited for mechanical/hydrodynamic perceptions in those copepods which lack a feeding current and hardly move. Numerous mechanosensors arranged in three dimensions on the first antennae (A1) are required to perceive the precise location of moving prey at a distance (e.g. Oithona feeding on ciliates but also sinking particles). Those copepods which create a weak or intermittent feeding current can supplement nutrition with carnivory, which requires perception by the A1 (e.g. Centropages velificatus adults). These two groups require, in addition to perception of prey motion/location, rapid motion by their appendages (A1, second maxillae M2, etc.) to capture the prey. Nauplii, which satiate at far lower food levels than adults, have one of several means of food acquisition: encounter through forward motion, perception through feeding current, or perception of a moving food particle. The nearly continuous motion of most calanoid nauplii makes them vulnerable to predation because all three pairs of appendages are usually moving. Opposite are nauplii of cyclopoid and a few calanoid species which move only

  13. Overview and early highlights of the TAIGER project marine, active-source seismic program (Invited)

    NASA Astrophysics Data System (ADS)

    McIntosh, K. D.; van Avendonk, H. J.; Liu, C.; Hsu, S.; Lee, C.; Wang, T. K.; Wu, F. T.

    2009-12-01

    The marine active-source portion of the TAIGER (TAIwan GEodynamic Research) project took place during April-July 2009 using the R/V Marcus Langseth with support from a variety of Taiwanese ships used to deploy and recover ocean bottom seismographs (OBSs). Due to Taiwanese shiptime (Langseth) contribution, the active-source program was doubled from our original proposal. Over the course of three, ~month-long cruises, the Langseth produced seismic source points along >13,000 km of track line. This includes > 11,000 km of deep-penetration multichannel seismic reflection data (MCS), shots to ~269 OBS stations (Taiwanese and U.S.), and shots to ~280 temporary land seismic stations across Taiwan. During this comprehensive project the Langseth circled Taiwan and ventured far to the south and east. TAIGER data cover the passive margin SW of Taiwan to provide a “pre-collision” structural configuration of the subducting plate, while TAIGER MCS and OBS data acquired on transects south of Taiwan will provide an idea of the “pre-collision” structural configuration of the Manila trench subduction zone. We will compare these areas to the evolving crustal structure of the Taiwan collision, which will be analyzed with onshore/offshore seismic data recorded during TAIGER legs 1 and 2. These TAIGER crustal transects will elucidate crucial components and stages of the southwestward advancing collision. We were able to process all the MCS data during the acquisition cruises to preliminary stack and FK migration. In much of the area SW of Taiwan we observe deep reflections, likely marking Moho. Surprisingly, even at distances > 250 km south of the shelf edge, apparent basement crustal thickness is frequently 3+ s (two-way travel time) or about 9-11 km. We also obtained exciting results across both the Manila/Luzon and Ryukyu arc-trench systems. These subduction systems are primarily characterized by ample sediment supply and relatively fast convergence leading to young, rapidly

  14. Isolation, screening, and characterization of surface-active agent-producing, oil-degrading marine bacteria of Mumbai Harbor.

    PubMed

    Mohanram, Rajamani; Jagtap, Chandrakant; Kumar, Pradeep

    2016-04-15

    Diverse marine bacterial species predominantly found in oil-polluted seawater produce diverse surface-active agents. Surface-active agents produced by bacteria are classified into two groups based on their molecular weights, namely biosurfactants and bioemulsifiers. In this study, surface-active agent-producing, oil-degrading marine bacteria were isolated using a modified Bushnell-Haas medium with high-speed diesel as a carbon source from three oil-polluted sites of Mumbai Harbor. Surface-active agent-producing bacterial strains were screened using nine widely used methods. The nineteen bacterial strains showed positive results for more than four surface-active agent screening methods; further, these strains were characterized using biochemical and nucleic acid sequencing methods. Based on the results, the organisms belonged to the genera Acinetobacter, Alcanivorax, Bacillus, Comamonas, Chryseomicrobium, Halomonas, Marinobacter, Nesterenkonia, Pseudomonas, and Serratia. The present study confirmed the prevalence of surface-active agent-producing bacteria in the oil-polluted waters of Mumbai Harbor.

  15. Viewing marine bacteria, their activity and response to environmental drivers from orbit: satellite remote sensing of bacteria.

    PubMed

    Grimes, D Jay; Ford, Tim E; Colwell, Rita R; Baker-Austin, Craig; Martinez-Urtaza, Jaime; Subramaniam, Ajit; Capone, Douglas G

    2014-04-01

    Satellite-based remote sensing of marine microorganisms has become a useful tool in predicting human health risks associated with these microscopic targets. Early applications were focused on harmful algal blooms, but more recently methods have been developed to interrogate the ocean for bacteria. As satellite-based sensors have become more sophisticated and our ability to interpret information derived from these sensors has advanced, we have progressed from merely making fascinating pictures from space to developing process models with predictive capability. Our understanding of the role of marine microorganisms in primary production and global elemental cycles has been vastly improved as has our ability to use the combination of remote sensing data and models to provide early warning systems for disease outbreaks. This manuscript will discuss current approaches to monitoring cyanobacteria and vibrios, their activity and response to environmental drivers, and will also suggest future directions.

  16. Isolation and characterization of multifunctional Streptomyces species with antimicrobial, nematicidal and phytohormone activities from marine environments in Egypt.

    PubMed

    Rashad, Ferial M; Fathy, Hayam M; El-Zayat, Ayatollah S; Elghonaimy, Ahlam M

    2015-06-01

    Different strategies have been employed for selective isolation of Streptomycetes from 20 marine samples varied in their biological nature. The recovery of Streptomycetes isolates (112) was influenced preferentially by different strategies; sediment samples were the best source of potential candidate Streptomycetes. All isolates exhibited antimicrobial activities with variable spectrum; the most promising isolates (31) were phenotypically characterized and identified as Streptomyces sp.; these isolates exhibited variable capacity for secretion of numerous hydrolytic enzymes such as catalase, protease, amylase, lipase, lecithinase, asparaginase, chitinase and pectinase. All the strains resisted both penicillin and streptomycin, 29 were sensitive to neomycin; the majority of strains (25) showed multiple antibiotic resistance index greater than 0.2; 23, 22 and 13 degraded the shrimp shell, chicken feather and corn cob, respectively, producing bioactive substance(s) which indicates their diversity and their ecological role in the marine ecosystem. At least 28 strains exhibited nematicidal activity in vitro and in vivo against root-knot nematode and supported plant growth. In vitro, the assessed Streptomyces species exhibited the ability to produce gibberellic acid, indole acetic acid, abscisic acid, kinetin and benzyladenine. Except for indole acetic acid, this is the first report concerning the ability of marine Streptomyces to produce such phytohormones and the use of shrimp shell waste as a mono component medium for production of phytohormones. The study is efficacious in selecting effective biodiverse strains of marine Streptomyces that may work under diverse agro-ecological conditions as a useful element in plant nutrition and as biocontrol agents involved in integrated management programs. PMID:25805507

  17. Fibrinogenolytic and anticoagulant activities in the tissue covering the stingers of marine stingrays Dasyatis sephen and Aetobatis narinari.

    PubMed

    Kumar, Kalainesan Rajesh; Vennila, Rathinam; Kanchana, Shankar; Arumugam, Muthuvel; Balasubramaniam, Thangavel

    2011-05-01

    Stingray envenomation is one of the major problems in the marine and freshwater ecosystem. Accidents in human cause immediate, local and intense pain, erythema, edema, hemorrhage, tissue necrosis and secondary bacterial infection are also common. To determine the effect of two marine stingray species Dasyatis sephen and Aetobatis narinari venom extract on coagulation, fibrin(ogen)olytic, proteolytic activities. Plasma coagulation, Thrombin catalyzed fibrinocoagulation, Fibrin plate assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), substrate SDS-PAGE and thrombin like activity by using chromogenic substrate were used to determine the effect of venom on plasma coagulation, its fibrin(ogen)olytic and proteolytic activity. The results show the presence of fibrin(ogen)olytic, anticoagulant and gelatinolytic activity in both stingray venom extracts. D. sephen venom delays coagulation of citrated plasma more significantly than A. narinari upon using increasing concentration of the venom. The same results were obtained in the fibrinocoagulation assays. SDS-PAGE analysis of fibrinogen and fibrin after incubation with D. sephen and A. narinari venom show fibrin(ogen)olytic activity. Through SDS-PAGE analysis it is confirmed that the delaying in coagulation process by stingray venom is due to its fibrin(ogen)olytic activity and fibrinolytic activity also confirmed through fibrin plate assay. Zymogram analysis shows the presence of array of gelatinolytic and fibrinogenolytic enzymes above 43-276 kDa in the D. sephen and A. narinari venom respectively. Protease inhibitor studies show the serine and metallo proteases are responsible for these activities. From the results, fibrinogenolytic, proteolytic activity of the stingray venom is confirmed, but it has no thrombin like activity and these activities may aid in hemorrhages, tissue necrosis and secondary bacterial infections at the site of envenomation. PMID:21165674

  18. Measurement of gas/water uptake coefficients for trace gases active in the marine environment

    SciTech Connect

    Davidovits, P. . Dept. of Chemistry); Worsnop, D.W.; Zahniser, M.S.; Kolb, C.E. . Center for Chemical and Environmental Physics)

    1992-02-01

    Ocean produced reduced sulfur compounds including dimethylsulfide (DMS), hydrogen sulfide (H{sub 2}S), carbon disulfide (CS{sub 2}), methyl mercaptan (CH{sub 3}CH) and carbonyl sulfide (OCS) deliver a sulfur burden to the atmosphere which is roughly equal to sulfur oxides produced by fossil fuel combustion. These species and their oxidation products dimethyl sulfoxide (DMSO), dimethyl sulfone (DMSO{sub 2}) and methane sulfonic acid (MSA) dominate aerosol and CCN production in clean marine air. Furthermore, oxidation of reduced sulfur species will be strongly influenced by NO{sub x}/O{sub 3} chemistry in marine atmospheres. The multiphase chemical processes for these species must be understood in order to study the evolving role of combustion produced sulfur oxides over the oceans. We have measured the chemical and physical parameters affecting the uptake of reduced sulfur compounds, their oxidation products, ozone, and nitrogen oxides by the ocean's surface, and marine clouds, fogs, and aerosols. These parameters include: gas/surface mass accommodation coefficients; physical and chemically modified (effective) Henry's law constants; and surface and liquid phase reaction constants. These parameters are critical to understanding both the interaction of gaseous trace species with cloud and fog droplets and the deposition of trace gaseous species to dew covered, fresh water and marine surfaces.

  19. 77 FR 4014 - Takes of Marine Mammals Incidental to Specified Activities; Physical Oceanographic Studies in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-26

    ...-listed marine mammals that may potentially occur in the survey area, blue and southern right whale... well known in the southern hemisphere. However, no take of blue whales was requested because of the low... the blue, fin, humpback, sei, southern right, and sperm whales. Under section 7 of the ESA, the...

  20. 78 FR 40698 - Takes of Marine Mammals Incidental to Specified Activities; Office of Naval Research Acoustic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-08

    ... depleted under the MMPA: blue whale (Balaenoptera musculus), fin whale (Balaenoptera physalus), gray whale... Their Status Common name Scientific name ESA and MMPA status Mysticetes Blue Whale Balaenoptera musculus... Marine mammal species harassment harassment Level B harassment Mysticetes Blue Whale 0.0000 0.0156...